LOCUS       DBERDK010000001        80189 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.001, whole genome shotgun sequence.
ACCESSION   DBERDK010000001 DBERDK010000000
VERSION     DBERDK010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 80189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80189
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.001"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..1115
                     /locus_tag="ACL73L_000001"
     CDS             <1..1115
                     /locus_tag="ACL73L_000001"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HGS0771062.1"
                     /translation="YVFNDDIASEWFLMMNKRLKTIQSEIVNVKIVDHVLKKFHLPLK
                     NLSYATFFRYFIPNFVKESRALYLDSDIIVTGSLDYLFDIELDGYALAAVEDSFGDVP
                     STNFNSGMLLVNVDTWRDEDACSKLLELTNQYHETAYGDQGILNMLFHDRWKRLDRNF
                     NFMVGMDSVAHIEGNHKWYEISELKNGDLPSVIHYTGVKPWEIISNNRFREVWWFYNL
                     LEWSDILLRKDIISRSFEELVYSPKAHTAIFTASCEMEHVEYLIENLPEVHFSILAHT
                     YFASSVVALLRYSNVTIYPCFSPFDYRKILDNLDFYLDINHYKEVDNIVSVVQQLSKP
                     IFTFENTSHDIGNQTNIFSSTEPNKMVEAIRQFIGE"
     gene            1120..1392
                     /locus_tag="ACL73L_000002"
     CDS             1120..1392
                     /locus_tag="ACL73L_000002"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGS0771063.1"
                     /translation="MADELISIVVPIYNVENYLRMCLDSIQNQTYQNFECLLINDGSP
                     DHSSKICEEFVEKDSRFKYFEKANGGLSSARNLGIECSGGGRTLLL"
     gene            1407..1997
                     /locus_tag="ACL73L_000003"
     CDS             1407..1997
                     /locus_tag="ACL73L_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HGS0771064.1"
                     /translation="MEHDALDRLYGALKKENADISIGRYNSYDETRYVYMTYVTDPDD
                     SLEVIEGKAIMDREGVEEVRNGNWTVAVLKLFKRELLQDLPFPIGKIAEDTYWTWKVL
                     LRASRIVYLNRCVYWYRVGLSDTLSNTWSEKRMYDEIGAREEKIAILASSDYDLTNHI
                     LIYKNRLQRVIAKLEEQNMQFTEIYRRMMEKLSLLP"
     gene            2026..3036
                     /locus_tag="ACL73L_000004"
     CDS             2026..3036
                     /locus_tag="ACL73L_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HGS0771065.1"
                     /translation="MKLHLTNLYGMAGDSTVILAQNAVQKIASQLGFREVGIYFYNIA
                     SDSPSEMNKRLDGIMASISIGDILVFQSPTWNGFEFDRLLFDKLKDMQVKIICFIHDV
                     VPLMFDSNYYLMKDYMYMYNLSDVLIVPSERMKTRLMEEGLTTKKILVQGMWDHPHDL
                     SLYTPAFKKELFFAGSLERFPDLQNWSQDTPLRVFSNKGEASSSARNLSIEGWKKDEE
                     LLLELSKGGFGLVWGTHQNEGESNQYYTLNISHKVSTYLTAGIPVIVPSSLSTAEFIV
                     DQGLGFVANSLEEVHEIVDKMNLQEYQEMTNRIKTFSYLLKEGYFTKKLFVDAIYHLG
                     VE"
     gene            3046..5490
                     /locus_tag="ACL73L_000005"
     CDS             3046..5490
                     /locus_tag="ACL73L_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_1767 family glycosyltransferase"
                     /protein_id="HGS0771066.1"
                     /translation="MIKTIVLAGDRNYIRQLETTIKSILYHNRDVKIYILNQDIMPDW
                     FRKPRKIARMLGSEIIDVKLPEQTVFQDWEKQDHISSITYARYFIADYIQEDKVLYLD
                     SDLIVNTSLEKLFSICLEEKSLAAVKDTDGITFNAGVLLINNKKWRQEKLKERLIEQS
                     IVTMKEVEEGRFEHFNGDQTIFNQVLQDDWLELGRAYNLQVGHDVVALYNNWQEHLAF
                     NDKPAVIHFTTYRKPWTTLIANRYRDLWWEFHDLEWNQILQHHIGEFEMTSPLDKEFS
                     CLTLTNSQDLEGIEELVTALPEVVFHIAAWTDMGDKLKKLAVYNNVRLHPQIVPPVLD
                     KLKKSTNLYLDINHGSADENFLKSLQEQEKTLLAFQSTQHGELGQIVFENGKVSFMID
                     TIKDFKKNGHLTCFRQLPSLTCLTFTASQDIEQLDYLAGQLPNVVFQVAAWTAMGPKL
                     YDLSNRYPNIQLYPAISRDKLDELKEKMDAYLDINLLTSTSDIVAEMAHLSKPILAFY
                     KSQNGNNGQRLYSSEHPERMLADLQKLITKDMLEKPLDIIQVKGIDETLDYIIEHNSS
                     LVRFGDGEVNLMWGLPIPYQNHDLELANQLKHIVGFESDEKLVVCLPDAFDDRFVFTW
                     WATPFWKEHMNVYMDFYKELCKGSWYGSTFISRPYIDYEDKSKAKGQFEKLKSIWENR
                     DILIVEGITSRSGVGNDLFDKVKSVKRIICPSHNAYSVVDNIQEEIMKHAEGRLILCM
                     LGPTAKVLSYNLCQMGYQVLDVGHVDSEYEWMKMGAKTKVKFSHKHTAEHNFDQDIEF
                     IDDETYNSQIVARILN"
     gene            5521..6741
                     /locus_tag="ACL73L_000006"
     CDS             5521..6741
                     /locus_tag="ACL73L_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGS0771067.1"
                     /translation="MRNTKRAVVFAGDYAYIRQIETAMKSLCRHNSHLKIYLLNQDIP
                     QEWFSQIRIYLQEMGGDLIDCKLIGSQFQMNWSNKLPHINHMTFARYFIPDFVTEDKV
                     LYLDSDLIVTGDLTDLFELDLGENYLAAARSCFGAGVGFNAGVLLINNKKWGSETIRQ
                     KLIDLTEKEHENVEEGDQSILNMLFKDQYSSLEDQYNFQIGYDYGAAAFKHQFIFDIP
                     LEPLPLILHYISQDKPWNQFSVGRLREVWWEYSLMDWSVILNEWFSKSVKYPSKSQIF
                     KLQCVNLTNSWCVEKIDYLAEQLPEVHFHIVAYTNMANELLALTRFPNVTVYPNSLPM
                     LLEQIVIASDLYLDLNHDRKLEDAYEFVLKYKKPMIAFDNTCSENLSEISYEGIYPSS
                     IPKKMVAAIRSYMR"
     gene            6750..7946
                     /locus_tag="ACL73L_000007"
     CDS             6750..7946
                     /locus_tag="ACL73L_000007"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HGS0771068.1"
                     /translation="MRKSIVLAADNAYLIPLETTIKSVLYHNRDVDFYILNSDIAPEW
                     FKLLGRKTEVVNSTIRSVHIDKELFESYKTGPHINYASYFRFFVTEVVESDRVLYLDS
                     DIIVTGELATLFEIDLKGYSIGAVDDVYAYEGRKSGFNAGMLLMDVVKWKEHSIVNSL
                     LELAAEQNQVVHLGDQSILNIYFEDNWLALDKTYNYMVGVDIYHLAQECERLDDNLPT
                     IVHYASHDKPWNTYSISRLRELWWVYRDLDWSEIAFQRSDLNYFERSNQSKKQVMFVT
                     WSADIKHLEYLVQRLPDWHFHLAAPCDCSEELISLSQYTNVTVYQNVLHSRIDWLLDD
                     SIVYLDINTGGEVFNVVTRAQESGKKIFAFDITRKSMDDGLYDGIFSVERPDDLVDRM
                     KNIEIE"
     gene            7946..8848
                     /locus_tag="ACL73L_000008"
     CDS             7946..8848
                     /locus_tag="ACL73L_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGS0771069.1"
                     /translation="MSELISVVVPIYNTGKYLVGCVEHILKQTYQNIEIILVDDGSTD
                     NSGEICDAFMMQDNRVRVLHQENKGAAQAKNMGISVAKGEYITIVDSDDIVKENMIET
                     LYQQVQEKDADVVIGNYYNYDESDGNFYFYVTGQDFCVEELAIQEIMNRQAGDWKFNS
                     SAFILPTFKLIKKELFNEVHFSNGRRFDDEATMHRFYLLASKIVFINDNLYLYRRRSG
                     SIMRTEFDLSWARDIVEVFSKKISDCVLAGLDVSVLRIRFVNLLKDYKQTLEYHQLTD
                     TEEYKDICFRLKLFFDAEQRNGKS"
     gene            8905..10122
                     /gene="secY2"
                     /locus_tag="ACL73L_000009"
     CDS             8905..10122
                     /gene="secY2"
                     /locus_tag="ACL73L_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HGS0771070.1"
                     /translation="MTKIYSSIAVKKGLFTLFLLFIYVLGSRITLPFVDLNTKDFLGG
                     STAYLAFSAALTGGNLRSLSIFSVGLSPWMSAMILWQMFSFSKRLGLTSTSIEIQDRR
                     KMYLTLMIAVIQSLAVSLRLPVQSSYSAILVVLMNTILLIAGTFFLVWLSDLNASMGI
                     GGSIVILLSSMVLNIPQDVLETFQTVHIPTGIIVLLALLTLVFSYLLALMYRARYLVP
                     VNKIGLHNRFKRYSYLEIMLNPAGGMPYMYVMSFLSVPAYLFILLGFIFPNHSGLAAL
                     SKEFMIGKPLWVYVYISVLFLFSIIFAFVTMNGEEIADRMKKSGEYIYGIYPGADTSR
                     FINRLVLRFSVIGGLFNVVMAGGPMLFVLFDEKLLRLAMIPGLFMMFGGMIFTIRDEV
                     KALRLNETYRPLI"
     gene            10132..11712
                     /gene="asp1"
                     /locus_tag="ACL73L_000010"
     CDS             10132..11712
                     /gene="asp1"
                     /locus_tag="ACL73L_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HGS0771071.1"
                     /translation="MYYFIPAWYGSERTWHADITPWYFSHFRLEFDDTFHQIRLFQEQ
                     DIDSRLLVLAYQPHLRYFLYRHGVLETDTYSVFDVMQDFHNLHTQVLSIRDIEWDDDC
                     EFIYSPFTIIVQKNGKKFAKVEHGVEGFISDIQYFGPNGQIHMHYIMDDRGFISSIIF
                     FEDGQAAYQEYLNPKGVWQFRERLKEGGQVEVNPILGYRFKMLTYQNMGDLVAEFFEN
                     YLQTYVKDQDIFMLPSHSHHDQLVLDRLPSTNPKLLSLFIGRNPQDTFRDLDVTFEKS
                     DLILVDREDSLRLLQELYPERMHQCYHLSSFDTRLRLGRSQTKKESIIYFQLDFEQGI
                     DNQALLQVLSFVAENKDTEVIFGAFAASQEQMNEVEGIVESFIQENIQSENLGKAIDY
                     GDAENPLEENQHQDLRLQFVNLNDELDLIKTLEFVRLIVDLNRHPHLYTQIAGISAGI
                     PQINLVETVYVEHLKNGYLLADVTEFSKAAHYYTDRLKEWNESLIYSIDKIKEHTGQQ
                     FLGKLEKWIEEVKNVKGT"
     gene            11696..13231
                     /gene="asp2"
                     /locus_tag="ACL73L_000011"
     CDS             11696..13231
                     /gene="asp2"
                     /locus_tag="ACL73L_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HGS0771072.1"
                     /translation="MSKELNILQIGLANWENHYDIPENMSWYYFYPNSSKALREIIEK
                     EDINRFHAVLIEDGQYSRDLFSYVKYFEPYTLFYNQNLQINDREVVDFLKKRCAQAID
                     FLSPQQLINDLSKSLFGGGYGDKLFPPTIQVNPNFTGAISYQGLDYVSLEGEFGQDFA
                     QLAYWAYNIMVQKTLPIELWLEYEKEGNCDFRLVIRKMWSGSVDDFFEEVIVSEKDLE
                     QALFMDSRDGDYFLSISVEARGRGTIKLGNLHQRWSRKQFGKFVLGGNILHDSKRDEI
                     NYFFHPGDFKPPLTVYFAGYRPAEGFEGYFMMKTLGCPFILFSDPRLEGGAFYLGTDE
                     LEGKVKDTITHYLDYLGFDHKDLILSGLSMGTFPALYYGASFEPHAIIVGKPLANLGT
                     IASRGRLDAPGVSNLAFDCLIHHTGGTSSQDMTELDQRFWKIFKQANFSKTTFGLSYM
                     KDEEMDPQAYEQLVSYLCNTGAKILSKGTAGRHNDDTDTNISWFLHFYRMVLETGFGR
                     EKR"
     gene            13228..13668
                     /gene="asp3"
                     /locus_tag="ACL73L_000012"
     CDS             13228..13668
                     /gene="asp3"
                     /locus_tag="ACL73L_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HGS0771073.1"
                     /translation="MIITQRQSIYWGEVGGTYMYGTTVSYYPDKSVRLYNPLLPSGEI
                     LKTWFSSVNYQAARTQPQLPLLKRKQEYQLSLVFDCQPENGVYTKITFFDRYGDILEK
                     KVEKAKDFIFTYPEDSYTYQVSLLSAGFESLTFYHFSIKEIRSV"
     gene            13661..16033
                     /gene="secA2"
                     /locus_tag="ACL73L_000013"
     CDS             13661..16033
                     /gene="secA2"
                     /locus_tag="ACL73L_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HGS0771074.1"
                     /translation="MFRRLGQDFQLRKVKKILKQINALKGKMSSLSDQELVAKTVEFR
                     QRLSEGESLDDILVEAFAVVREADKRILGMFPYDVQVMGAIVMHYGNVAEMNTGEGKT
                     LTATMPVYLNAFSGEGVMVVTPNEYLSKRDAEEMGQVYRFLGLTIGVPFTEDPKKEMK
                     AEEKKLIYASDIIYTTNSNLGFDYLNDNLASNEEGKFLRPFNYVIIDEIDDILLDSAQ
                     TPLIIAGSPRVQSNYYAIIDTLVTTLVEGEDYIFKEEKEEVWLTTKGAKSAENFLGID
                     NLYKEEHASFARHLVYAIRAHKLFTKDKDYIIRGNEMVLVDKGTGRLMEMTKLQGGLH
                     QAIEAKEHVKLSPETRAMASITYQSLFKMFNKISGMTGTGKVAEKEFIETYNMSVVRI
                     PTNRPRQRIDYPDNLYITLPEKVYASLEYIKQYHAKGNPLLVFVGSVEMSQLYSSLLF
                     REGIAHNVLNANNAAREAQIISESGQMGAVTVATSMAGRGTDIKLGKGVAELGGLIVI
                     GTERMESQRIDLQIRGRSGRQGDPGMSKFFVSLEDDVIKKFGPSWVHKKYKDYQVQDM
                     TQPEVLKGRKYRKLVEKAQHASDSAGRSARRQTLEYAESMNIQRDIVYKERNRLIDGS
                     RDLEDVVVDIIERYTEEVAADHYASRELLFHFIVTNISFHVKEVPDYIDVTDKTAVRS
                     FMKQVIDKELSEKKELLNQHDLYEQFLRLSLLKAIDDNWVEQVDYLQQLSMAIGGQSA
                     SQKNPIVEYYQEAYAGFEAMKEQIRADMVRNLLMGLVEVTPKGEIVTHFP"
     gene            16045..17556
                     /gene="gtfA"
                     /locus_tag="ACL73L_000014"
     CDS             16045..17556
                     /gene="gtfA"
                     /locus_tag="ACL73L_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HGS0771075.1"
                     /translation="MTIYNINLGIGWASSGVEYAQAYRAGVFRKLNLSSKFIFTDMIL
                     ADNIQHLTANIGFDDNQVIWLYNHFTDIKIAPTSVTVDDVLAYFGGEESHREKNGKVL
                     RVFFFDQDKFVTCYLVDENKDLVQHAEYVFKGNLIRKDYFSYTRYCSEYFAPKDNVAV
                     LYQRTFYNEDGTPVYDILMNQGKEEVYHFKDKIFYGKQAFVRAFMKSLNLNKSDLVIL
                     DRETGIGQVVFEEAQTAHLAVVVHAEHYSENATNEDYILWNNYYDYQFTNADKVDFFI
                     VSTDRQNEVLQEQFAKYTQHQPKIVTIPVGSIDSLTDSSQGRKPFSLITASRLAKEKH
                     IDWLVKAVIEAHKELPELTFDIYGSGGEDSLLRDIIANHQAEDYIQLKGHAELSQIYS
                     QYEVYLTASTSEGFGLTLMEAIGSGLPLIGFDVPYGNQTFIEDGQNGYLIPSSSDHVE
                     DQIKQAYAAKICQLYQENRLEAMRAYSYQIAEGFLTKEILEKWKKTVEEVLHD"
     gene            17549..18886
                     /gene="gtfB"
                     /locus_tag="ACL73L_000015"
     CDS             17549..18886
                     /gene="gtfB"
                     /locus_tag="ACL73L_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031647 - regulation of protein stability
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HGS0771076.1"
                     /translation="MIELYDRYSHESRDLHESLVATGLSQLGVVIDADGFLPDGLLSP
                     FTYYLGYEDGKPLYFNQVPVSDFWEILGDNQSACIEDVTQERAVIHYADGMQARLVKQ
                     VDWKDLEGRVRQVDHYNRFGTCFAKTTYSADSEPIMTVYQDVNGQQVLLENHVTGDIL
                     LTLPGQSMRYFANKVEFITFFLQDLEIDTSQLIFNTLATPFLVSFHYPDKSGSDVLVW
                     QEPLYDAIPGNMQLILESDNVRTKKIIIPNKTTYERALELTDEKYHDQFVHLGYHYQF
                     KRDNFLRRDALILTNSDQIEQVEAIVEALPDVTFRIAAVTEMSSKLLDMLCYPNVALY
                     QNASPQKIQELYQLSDIYLDINHSNELLQAVRQAFEHNLLILGFNQTVHNRLYVAPDH
                     LFESSEVAALVETIKLALSDVDQMRQALGKQGQHANYVDLVRYQETMQTVLGG"
     gene            18888..19067
                     /gene="asp4"
                     /locus_tag="ACL73L_000016"
     CDS             18888..19067
                     /gene="asp4"
                     /locus_tag="ACL73L_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory secretory system protein Asp4"
                     /protein_id="HGS0771077.1"
                     /translation="MSEEDLFYKDVEGRMEELKQKPIKKEKETRGEKISKTFSLLLGL
                     MILIGLLFTLLGILR"
     gene            19072..19296
                     /locus_tag="ACL73L_000017"
     CDS             19072..19296
                     /locus_tag="ACL73L_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory secretory protein Asp5"
                     /protein_id="HGS0771078.1"
                     /translation="MIDILIVLAIILSLALIVLVTIQPRQNQLFSMDATSNIGKPSYW
                     QSNTLVKVLTLLVSLALFVLLLTFMVITYK"
     gene            19357..19509
                     /locus_tag="ACL73L_000018"
     CDS             19357..19509
                     /locus_tag="ACL73L_000018"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771079.1"
                     /translation="MKIKEQTRKLAAGCSKHGFEVVDRTDEVSSKHGFEVVDRTDEVS
                     NIYTAR"
     gene            complement(19532..20485)
                     /locus_tag="ACL73L_000019"
     CDS             complement(19532..20485)
                     /locus_tag="ACL73L_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HGS0771080.1"
                     /translation="MSISQRTTKLILATCLACLLAYFLNLSSAVSAGIIALLSLSDTR
                     RSTLKLARNRLFSMLLALAIGVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFLLFAIGTGFALLVNLYMPSREEEIQHYHTLVEEKLK
                     DILQRFKYYLSRGDGRNRAQLVAELDTLLKEALRLVYLDHSDHLFHQTDYHIHYFEMR
                     QRQSRILRNMAQQINTCHLAASESLILAQLFSKIAGQLSQTNPASDLLDEIERYLEVF
                     RNRSLPKTREEFETRATLLQLLREAKTFIQVKVDFYQKYRQ"
     gene            complement(20553..21762)
                     /gene="sstT"
                     /locus_tag="ACL73L_000020"
                     /pseudo
     CDS             complement(20553..21762)
                     /gene="sstT"
                     /locus_tag="ACL73L_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            21901..22461
                     /locus_tag="ACL73L_000021"
     CDS             21901..22461
                     /locus_tag="ACL73L_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HGS0771081.1"
                     /translation="MQKFIQAYIEKLDVTTIIENILTKVISLLLLLIVFYIAKKMLHT
                     MVQRIVKPSLKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGA
                     GIAGVAIGMGAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGIRTTQ
                     LRSEEQALHFVPNRNITVVSNFSRTD"
     gene            22518..23426
                     /locus_tag="ACL73L_000022"
     CDS             22518..23426
                     /locus_tag="ACL73L_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HGS0771082.1"
                     /translation="MVLEKQLGNGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESGTVTFIYNVLDVKKDKAYYETFPMTFIVEHRRLITISNTKNAYVIE
                     QMTRYLENHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNLFA
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTDEPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            23441..23968
                     /locus_tag="ACL73L_000023"
     CDS             23441..23968
                     /locus_tag="ACL73L_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HGS0771083.1"
                     /translation="MAIENYIPDFAVEAVYDLTVPSLQAQGIKAVLVDLDNTLIAWNN
                     PDGTPEMKQWLHDLRDAGIGIIVVSNNTKKRVQRAVEKFGIDYVYWALKPFTFGIDRA
                     MKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKPLVQHDSIKTQINRTRERRVMR
                     KITEKYGPITYKKGI"
     gene            23971..25077
                     /gene="yqeH"
                     /locus_tag="ACL73L_000024"
     CDS             23971..25077
                     /gene="yqeH"
                     /locus_tag="ACL73L_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HGS0771084.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTNDDFLKLLHEVGDSDALVVNVIDIFDFNGSVIPGLPRFVSGNDV
                     LLVGNKKDILPKSVKSGKISQWLMKRAHEEGLRPVDVVLTSAQNKHAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFIAGEKQ
                     GFTAFFDNELKLHRSKLEGASAFYDKHLGTLLTPPNSKEKEDFPRLVQHVFTIKDKTD
                     LVISGLGWIRVTGIAKVAVWAPEGVAVVTRKAII"
     gene            25292..25603
                     /gene="yhbY"
                     /locus_tag="ACL73L_000025"
     CDS             25292..25603
                     /gene="yhbY"
                     /locus_tag="ACL73L_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HGS0771085.1"
                     /translation="MSLTSKQRAFLNSQAHTLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            25655..26284
                     /locus_tag="ACL73L_000026"
     CDS             25655..26284
                     /locus_tag="ACL73L_000026"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HGS0771086.1"
                     /translation="MTIELLTPFTKVELEPEIKEKKRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLMPEYQPPHVDKKETIPEHHRLKMLELAIEGIDGLVIETIELERK
                     GISYTYDTMKILTEKNPDTDYYFIIGADMVDYLPKWYRIDELVDMVQFVGVQRPRYKA
                     GTSYPVIWVDVPLMDISSSMVRAFLAQGRKPNFLLPQLVLDYIEKEGLY"
     gene            26284..26877
                     /gene="yqeK"
                     /locus_tag="ACL73L_000027"
     CDS             26284..26877
                     /gene="yqeK"
                     /locus_tag="ACL73L_000027"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HGS0771087.1"
                     /translation="MSYQDYINCSREALLEKMAELLPEKRLTHCLGVERAAMELAQRF
                     GVDVEKASLAGLLHDYAKKLSDQEFLDLIDRYQLDPDLKNWGNNVWHGMVGIYKIQED
                     LDLHDSEILRAIEVHTVGAGQMTDLDKVVYVADYIEHNRSFPGVDVAREIASLSLNKA
                     VAYETARTVEYLAHQGFPIYPQTLETYNAFVHYLKED"
     gene            26878..27378
                     /locus_tag="ACL73L_000028"
     CDS             26878..27378
                     /locus_tag="ACL73L_000028"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HGS0771088.1"
                     /translation="MKTAFIIIDVQNVLVETGFQTKSLLEKISYLQNQARSKNIEIIY
                     VQHIENSEAQTSEGWQLSALLNRKPAEKVFQKKYNSIFKETGLKEYLDKQGIEKLVLC
                     GMQTEYCVDTSVKVAFEYGYQLIVPEGAVTTFDGDDIPAETINEFYEDIWEERFADVL
                     DYKHIF"
     gene            27391..27744
                     /gene="rsfS"
                     /locus_tag="ACL73L_000029"
     CDS             27391..27744
                     /gene="rsfS"
                     /locus_tag="ACL73L_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HGS0771089.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALDVQDLTSVTDYFVITSSMN
                     SRQLDAIAANIREKVAQAGFKGSHVEGDAAGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEANSVDISEALA"
     gene            27853..28593
                     /locus_tag="ACL73L_000030"
     CDS             27853..28593
                     /locus_tag="ACL73L_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGS0771090.1"
                     /translation="MATYETFAAVYDAVMDDSLYDKWTNFSLRHLPKTKERKKLLELA
                     CGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKIAFIEGNMLNLSKAGKYD
                     FVTCYSDSICYMQDEVEVGDVFKDVYNVLNEEGVFIFDVHSTYQTDEVFPGYSYHENA
                     EDFAMLWDTYEDEVPHSIVHELTFFIKEADGSFSRHDEVHEERTYEILTYDILLEQAG
                     FKSFKLYADFEDKEPTETSTRWFFVAQK"
     gene            28603..29700
                     /locus_tag="ACL73L_000031"
     CDS             28603..29700
                     /locus_tag="ACL73L_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HGS0771091.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLDQAEGLKIVAMSGNFMQRGEPAI
                     VDKWTRAQMALENGADLVVELPFLVSVQAADFFGQGAVDILDRLGIDSLVFGTEEVRD
                     YQKIADLYTEKGTEMEKFVENLPDSLSYPQKTQAMWKEFAGLDFSGNTPNHVLALAYA
                     KAVAGRNIKLHPIQRQGAGYHSVNKDVDFASATALRQHQKDQDFLERFMPSVALFEQA
                     SKVIWEDYFPLLRYQILSNPDLTTIYQVNQEMAVRIKEAIKTAQSVEELVELVTTKRY
                     TKARVRRLLTYILMQARESDLPEAIHVLGFTEKGRQHLKSLKGQVSLVSRIGKEPWDA
                     MTQKADQIYQLGKPSIAQQNFGRVPIRIETN"
     gene            29938..30192
                     /locus_tag="ACL73L_000032"
     CDS             29938..30192
                     /locus_tag="ACL73L_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HGS0771092.1"
                     /translation="MEAVLYSTFRNHLKDYMKKVNDEFEPLTVVNKNPDEDIVVLSKS
                     EWDSIQETLRIAQNKELSDKVLRGMAQVRAGSTQVHVIEE"
     gene            30196..30450
                     /locus_tag="ACL73L_000033"
     CDS             30196..30450
                     /locus_tag="ACL73L_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HGS0771093.1"
                     /translation="MLLKFTEDAWADYCYWQNQDKKTLKRINKLIKDIQRDPFTGIGK
                     PEPLKYDYQGAWSRRIDAENRLIYMMDGDSVAFLSFKDHY"
     gene            30641..32254
                     /locus_tag="ACL73L_000034"
     CDS             30641..32254
                     /locus_tag="ACL73L_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HGS0771094.1"
                     /translation="MEIMSLAIAVFAVIIGLVIGYVSISAKMKSSQEAAELMLLNAEQ
                     EATNLRGQAEREADLLVNEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERQKEAE
                     LERIGALSQAEARDIILAQTEENLTREIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTMEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNTIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
     gene            32384..33010
                     /gene="gmk"
                     /locus_tag="ACL73L_000035"
     CDS             32384..33010
                     /gene="gmk"
                     /locus_tag="ACL73L_000035"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HGS0771095.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALM
                     REYDYAIVNDQVPLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            33035..33349
                     /gene="rpoZ"
                     /locus_tag="ACL73L_000036"
     CDS             33035..33349
                     /gene="rpoZ"
                     /locus_tag="ACL73L_000036"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HGS0771096.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHPDPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            33415..35811
                     /locus_tag="ACL73L_000037"
     CDS             33415..35811
                     /locus_tag="ACL73L_000037"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HGS0771097.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVFGLESQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKIS
                     ILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLGKQAEMMRLTRK
                     GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLAS
                     LLESYSREQVNFFVDQGAVTIVQKEVRRSAAYFEGIEASRPLELNPEQRQARDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEAAYK
                     QDSNPRYHAREVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQVVLMLNRRGYSSFVMCRECG
                     TVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSHSIRYYGTGTQKAYDEL
                     AELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQLLTQVAGRAGRAEKAGQVLIQSYNSQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKRAYEVMNILRSGLSETSPILG
                     PTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            35824..36759
                     /gene="fmt"
                     /locus_tag="ACL73L_000038"
     CDS             35824..36759
                     /gene="fmt"
                     /locus_tag="ACL73L_000038"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HGS0771098.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDNRYEILAVATQPDRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEDLMKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
                     NVGTLFEKLALVGRDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPEEEKLDWNKTN
                     RQLFNQIRGMNPWPVAHTFLKGDRFKIYEALPVEGQGNPGEILSICKKELIVATAEGA
                     LSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD"
     gene            36752..38065
                     /gene="rsmB"
                     /locus_tag="ACL73L_000039"
     CDS             36752..38065
                     /gene="rsmB"
                     /locus_tag="ACL73L_000039"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HGS0771099.1"
                     /translation="MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLSAADKG
                     LVTELVYGTVARKLTLEWYLSHFIEDRDQLDSWLYVLLLMSAYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNAVLRRILREGWPDIASIKRKNKRDSIAYSLPVWLVAKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANDSLLATTGLVKEQGHFAG
                     HNLFADGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGIEDRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            38080..38820
                     /locus_tag="ACL73L_000040"
     CDS             38080..38820
                     /locus_tag="ACL73L_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HGS0771100.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIIDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAHPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTE
                     TLVRFANNAGGLDNITVALVSMNEEDAE"
     gene            38817..40796
                     /gene="stkP"
                     /locus_tag="ACL73L_000041"
     CDS             38817..40796
                     /gene="stkP"
                     /locus_tag="ACL73L_000041"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase StkP"
                     /protein_id="HGS0771101.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENPSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIKNPSQAVTEETYQPQ
                     APKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKAT
                     TIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVS
                     KPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSAE
                     GMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP"
     gene            40911..41669
                     /locus_tag="ACL73L_000042"
     CDS             40911..41669
                     /locus_tag="ACL73L_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HGS0771102.1"
                     /translation="MEESKELNAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAQPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLMN"
     gene            41671..42120
                     /locus_tag="ACL73L_000043"
     CDS             41671..42120
                     /locus_tag="ACL73L_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HGS0771103.1"
                     /translation="MTLTTFLLQAVASFLAIITFLIVLNVQRSMLLPGGILGMTVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGMASGTVILRLYHYIKTHRVS"
     gene            42182..42808
                     /locus_tag="ACL73L_000044"
                     /pseudo
     CDS             42182..42808
                     /locus_tag="ACL73L_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            42876..43124
                     /locus_tag="ACL73L_000045"
     CDS             42876..43124
                     /locus_tag="ACL73L_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771104.1"
                     /translation="MVSSSGSEFQSGWQEHQLIAEKVSKTLDKTFDKDVREIPTSQFY
                     QKFVDEMGRTYSGNLILQELITVNGAYKATYIGELSSN"
     gene            43255..44427
                     /locus_tag="ACL73L_000046"
     CDS             43255..44427
                     /locus_tag="ACL73L_000046"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HGS0771105.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     APLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGN
                     IYTGSLFLGLLSLLENTDSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLDKDRLN
                     KLNQRTALSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK"
     gene            44427..45701
                     /locus_tag="ACL73L_000047"
     CDS             44427..45701
                     /locus_tag="ACL73L_000047"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HGS0771106.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEKDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNDQEYTVPYVTEEPSVVAAASYAIKIIKRTGGFT
                     AQVHQRQMIGQVALYQVANPKLAQEKIASKKAELLELANQAYPSIVKRGGGARDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLMGILSNYATDSL
                     VTASCRIAFRYLSRQKDQGREIAEKIALASQFAQADPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLDLEREELVGEMTLPMPVATKGGSIGLNPR
                     VALSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQAKSLALLAGA
                     SESEVAPLVERLISDKTFNLETAQRYLENLRS"
     gene            complement(45736..46701)
                     /locus_tag="ACL73L_000048"
     CDS             complement(45736..46701)
                     /locus_tag="ACL73L_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGS0771107.1"
                     /translation="MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     GYKPNNLARSLQGKSAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQSIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTREKPDAIFASDDLTAILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKSI"
     gene            complement(46682..48136)
                     /locus_tag="ACL73L_000049"
     CDS             complement(46682..48136)
                     /locus_tag="ACL73L_000049"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HGS0771108.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWAQLESDDLVHFKETGIKVLPDTPLDS
                     HGAYSGSAMQFGDNLFLFYTGNVRDENWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFKGQYYAVVGGQDLEKKGFVRLYKAVNNDYTNWQAVGDLDFANDRT
                     AYMMECPNLVFVEEHPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRALAVSWLGLPDVSYPSDRFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRASEEAFSNRSQTKNTYELELNLEANSQSEIVLLADKEGKGLSIN
                     FDLVNGQVTVDRSQAGEQYAQEFGTTRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGTYYELDYGRKTN"
     gene            48513..50396
                     /locus_tag="ACL73L_000050"
     CDS             48513..50396
                     /locus_tag="ACL73L_000050"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HGS0771109.1"
                     /translation="MNNQEIAKKVIDALGGRENVNSVAHCATRLRVMVKDEEKINKEV
                     IENLEKVQGAFFNSGQYQIIFGTGTVNKMYDEVVVLGLPTSSKDDMKAEVAKQGNWFQ
                     RAIRTFGDVFVPIIPVIVATGLFMGVRGLFNALEMPLPGDFATYTQILTDTAFIILPG
                     LVVWSTFRVFGGNPAVGIVLGMMLVSGSLPNAWAVAQGGEVTAMNFFGFIPVVGLQGS
                     VLPAFIIGVVGAKFEKAVRKVVPDVIDLLVTPFVTLLVMSILGLFVIGPVFHVVENYI
                     LIATKAILSIPFGLGGFLIGGVHQLIVVSGVHHIFNLLEVQLLAADHANPFNAIITAA
                     MTAQGAATVAVGVKTKNPKLKTLAFPAALSAFLGITEPAIFGVNLRFRKPFFLSLIAG
                     AIGGGLASILGLAGTGNGITIIPGTMLYVGNGQLPQYLLMVAVSFALGFALTYMFGYE
                     DEVDATAAAKQAEVAEEKEEVAPAALQNETLVTPIVGDVVALADVNDPVFSSGAMGQG
                     IAVKPSQGVVYAPADAEVSIAFPTGHAFGLKTRNGAEVLIHVGIDTVSMNGDGFEAKV
                     AQGNKVKAGDVLGTFDSNKIAAAGLDDTTMVIVTNTADYASVAPVATGSVAKGDAVIE
                     VKI"
     gene            50543..51430
                     /gene="scrK"
                     /locus_tag="ACL73L_000051"
     CDS             50543..51430
                     /gene="scrK"
                     /locus_tag="ACL73L_000051"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HGS0771110.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPM
                     YFTTDVNSSAYGEVVARNNAGGRIENLVYYTIGTGIGAGVIQRGEFIGGVGHPEMGHY
                     YVARHPMDIEKEFKGVCPFHKGCLEGYAAGPSLEARTGVRGETIELNNPVWDVQAYYI
                     AQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYIVTPAV
                     AGNGSATLGNFVLAKEVSK"
     gene            complement(51750..52367)
                     /locus_tag="ACL73L_000052"
     CDS             complement(51750..52367)
                     /locus_tag="ACL73L_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HGS0771111.1"
                     /translation="MSQDKQMKAVSPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQAGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIGWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQML"
     gene            complement(52782..52995)
                     /locus_tag="ACL73L_000053"
                     /pseudo
     CDS             complement(52782..52995)
                     /locus_tag="ACL73L_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(53363..53554)
                     /locus_tag="ACL73L_000054"
     CDS             complement(53363..53554)
                     /locus_tag="ACL73L_000054"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771112.1"
                     /translation="MKIKEQTRKLAVDCSKYSFEVADKTDEVSSKHCFEVVDRTDEVS
                     NYIYGKAKLTWFEEIFEEY"
     gene            53677..54570
                     /locus_tag="ACL73L_000055"
     CDS             53677..54570
                     /locus_tag="ACL73L_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0771113.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEIGLDGQDIRQETYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLEVKGKLADKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILEDESAGRELFPILTQGQYIA
                     TFDQQAPTIDEIFKLESGVEV"
     gene            54567..55766
                     /locus_tag="ACL73L_000056"
     CDS             54567..55766
                     /locus_tag="ACL73L_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS0771114.1"
                     /translation="MRNMWVVIKETYLRHVESWSFFFMVISPFLFLGISVGIGHLQGS
                     SMAKNNKVAVVTTVPSVAEGLKNVNGVNFDYKDEASAKEAIKEEKLKGYLTIDQEDSV
                     LKAVYHGETSLENGIKFEVTGTLNELQNQLNRSTASLSQEQEKRLAQTIQFTEKIDEA
                     KENKKFIQTIAAGALGFFLYMILITYAGVTAQEVASEKGTKIMEVVFSSIRASHYFYA
                     RMMALFLVILTHIGIYVVGGLAAVLLFKDLPFLAQSGILVHLGDAISLNTLLFILISL
                     FMYVVLAAFLGSMVSRPEDSGKALSPLMILIMGGFFGVTALGAAGDNLLLKIGSYIPF
                     ISTFFMPFRTINGYAEGAEAWISLAITVIFAVVATGFIGRMYASLVLQTDDLGIWKTF
                     KRALSYK"
     gene            complement(56119..56886)
                     /locus_tag="ACL73L_000057"
     CDS             complement(56119..56886)
                     /locus_tag="ACL73L_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771115.1"
                     /translation="MFWNLVHYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLAISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAAYIGIQIVIGFIELFFNLSSNFYVNSLVGL
                     NDHFYMGAGIAIVKELIFIAIFYLGTYYILRNKVNLL"
     gene            complement(56903..57598)
                     /locus_tag="ACL73L_000058"
     CDS             complement(56903..57598)
                     /locus_tag="ACL73L_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0771116.1"
                     /translation="MSLLAFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDMDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYVLDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            complement(57603..57968)
                     /locus_tag="ACL73L_000059"
     CDS             complement(57603..57968)
                     /locus_tag="ACL73L_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGS0771117.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     GVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMTH
                     FGYEKEELPGVVSDYIKGV"
     gene            58108..58581
                     /gene="nrdR"
                     /locus_tag="ACL73L_000060"
     CDS             58108..58581
                     /gene="nrdR"
                     /locus_tag="ACL73L_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HGS0771118.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            58582..59751
                     /locus_tag="ACL73L_000061"
     CDS             58582..59751
                     /locus_tag="ACL73L_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HGS0771119.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLVLHAPLSSQD
                     FLEHPVYRRLLEKKIGDVAVEDLKVESADGEEIPVSLNQVFPELAELGSQEDLGLKKK
                     VANDFDLDHFRQLMARDGLRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTAVSQVI
                     STKRMREKIAQKPVSSDFSLKETTIIKEAKSKTALQFLAEIKQTRKGTITQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEKAVLRIRER
                     GQKAKIQKQNQTAPVKTNVPKWSNPEYKNETSEETRLELERKKQELLARLEKGGD"
     gene            59752..60648
                     /gene="dnaI"
                     /locus_tag="ACL73L_000062"
     CDS             59752..60648
                     /gene="dnaI"
                     /locus_tag="ACL73L_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HGS0771120.1"
                     /translation="MESVGDVLKRQPSRFYYQDLVQKIMKDPDVAAFIQQESLTPEEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVKNHGYADVSYEETPELIAAEKEAA
                     IKNRLKLINLPASLKKASLAQVDLDDLGRLPVFEKLLAFVEQYPAIRKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAISDGNVKTLVDEIKLSEVLILD
                     DIGAEQSTVWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKVKHGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            60645..61358
                     /locus_tag="ACL73L_000063"
     CDS             60645..61358
                     /locus_tag="ACL73L_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HGS0771121.1"
                     /translation="MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     GLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYPVFGMAL
                     GVPNQTHDVKPRLPLDNVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQ
                     FGQAEPSSTRKNLEQKKLL"
     gene            61372..62682
                     /gene="der"
                     /locus_tag="ACL73L_000064"
     CDS             61372..62682
                     /gene="der"
                     /locus_tag="ACL73L_000064"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HGS0771122.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQ
                     RLHKLPEMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            62771..63106
                     /locus_tag="ACL73L_000065"
     CDS             62771..63106
                     /locus_tag="ACL73L_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771123.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWFYIPFY
                     SLFSLAWTVVSVLFFNTVLRNVTDIKLWGIWFLFILLAIGMNWLTYSCFKEMFRLRQE
                     LGKSKGGRH"
     gene            63246..63581
                     /locus_tag="ACL73L_000066"
     CDS             63246..63581
                     /locus_tag="ACL73L_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771124.1"
                     /translation="MEHLFKMIILLPCFYFFSWIDKDNRESKFFPIFYYFYWIYITLY
                     ALFSLAWTVFSVLFFNIVLRNLTDIKLWGIWLLLLLIAFASDWLDYICFKKMLDLRRE
                     LGKSKGGRH"
     gene            63766..64557
                     /locus_tag="ACL73L_000067"
     CDS             63766..64557
                     /locus_tag="ACL73L_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771125.1"
                     /translation="MKIPLLTLARHKFVYVLLTLLFLALVYRDVLMTYFFFDIHAPDL
                     AKFDGQAIKNDLLKSALDFRILQFNLGFYQSFIIPIIIVLLGFQYIELKNKVLRLSIG
                     REVSYQGLKRKLTLQVASIPCLIYLVTVLIIAILTHFFGTFSPLGWNSLFSDGSSLQR
                     LLDGEITSYLFFTCVLLIGIFINAVYFLQIVDYLGNVTRSAITYLMFLWLGSMLLYSA
                     LPYYMVPMTSLMQASYGDVSLMKLFTPYILYIVSYMVLEKYEDNV"
     gene            64550..65191
                     /locus_tag="ACL73L_000068"
     CDS             64550..65191
                     /locus_tag="ACL73L_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771126.1"
                     /translation="MFKNFNNILLKRKIVLLLRIVLMMILINHLLSTAVQKQDAVIFF
                     KRELISIFSYNDYSEVNLEIPKLLLNLSLFMVGWLSVILLESDLADHYHHLIRYQSSS
                     FFDYTRKRLVVISKFFTQDLFVWFLGLLPLGIHFKTVALFFLLAQLMMLYLLLSYLIA
                     LISAGAGFSFFLYFLAFVGQEWMMDHIVTVYLLLLSLLVMLIVSRLEEKFKKG"
     gene            65195..65827
                     /locus_tag="ACL73L_000069"
     CDS             65195..65827
                     /locus_tag="ACL73L_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGS0771127.1"
                     /translation="MRLEIINGQKIYRKRPILNQLNLVFQSGKIYGLKGDNGSGKTVL
                     LKILAGYIKLDKGKVLQDGKVYGVKNHYIQDAGILIEKVEFLSHLSLRENLELLRYFS
                     SKVTEKRIAYWIQYYDLQEFEDIEYRHLSLGTKQKMALIQAFISSPSILFLDEPMNAL
                     DEKSVRLTKQVILSYLKKENGLVILTSHISEDISDLCTDVLVVENGHIQM"
     gene            65855..66142
                     /locus_tag="ACL73L_000070"
     CDS             65855..66142
                     /locus_tag="ACL73L_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771128.1"
                     /translation="MAHLKSFITRYSKVYIGLVLLIWLSFFFIPWDKPLLGIRIDIFI
                     IQKILLAFGILSILMALLSKKVSLFVFGLICCLSLWINLFITLAILPIFGN"
     gene            66288..68801
                     /gene="secA"
                     /locus_tag="ACL73L_000071"
     CDS             66288..68801
                     /gene="secA"
                     /locus_tag="ACL73L_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HGS0771129.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMTALTDDQLKAK
                     TVEFKERYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERLKGIFERLNMSE
                     EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREIIYAQRYDVITADRDL
                     APEIQSMIKRTIERVVDGHARAKQDEKLEAILNFAKYNLLPEDSITMEDLSGLSDKAI
                     KEELFQRALKVYDSQVSKLRDEEAVKEFQKVLILRVVDNKWTDHIDALDQLRNAVGLR
                     GYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIHEQERPQAERHISTTATRN
                     IAAHQASMPEDLDLSQIGRNELCPCGSGKKFKNCHGKRQ"
     gene            68882..69913
                     /locus_tag="ACL73L_000072"
     CDS             68882..69913
                     /locus_tag="ACL73L_000072"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HGS0771130.1"
                     /translation="MVFTAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYAKRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLIDTIAQYEKMGLENPFIIIDTNHDNSGKQYIEQI
                     RIVRQTLINRAWNEKIKQFVRGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALV
                     REIYKTLGE"
     gene            69915..70946
                     /locus_tag="ACL73L_000073"
     CDS             69915..70946
                     /locus_tag="ACL73L_000073"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HGS0771131.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEIFGCSITDPCLGWENTEALV
                     EEIYVTLTK"
     gene            70991..71353
                     /gene="acpS"
                     /locus_tag="ACL73L_000074"
     CDS             70991..71353
                     /gene="acpS"
                     /locus_tag="ACL73L_000074"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HGS0771132.1"
                     /translation="MIVGHGIDIEELASIESAVTRHEGFAKRVLTAQEMERFTSLKGR
                     RQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLSIS
                     HTDQFVTASVILEENHES"
     gene            71343..72446
                     /gene="alr"
                     /locus_tag="ACL73L_000075"
     CDS             71343..72446
                     /gene="alr"
                     /locus_tag="ACL73L_000075"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HGS0771133.1"
                     /translation="MKASPHRPTKALIHLGAIRQNIQQMGAHIPQGTLKLAVVKANAY
                     GHGAVAVAKAIQDDVDGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFREASEVEQAQDLLQQHGVRVE
                     GIFTHFATADEESDDYFNAQLERFKTILASMKEVPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLDLPYDLIPALTLESALVHVKTVPSGACMGYGATYQADSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKPYPLGTKVTLIGSNG
                     DKEITATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            72465..74480
                     /gene="recG"
                     /locus_tag="ACL73L_000076"
     CDS             72465..74480
                     /gene="recG"
                     /locus_tag="ACL73L_000076"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HGS0771134.1"
                     /translation="MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     TKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEVVFAVNFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLTGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKDLAEYKQALRRIKFEELFYFQMQ
                     LQTLKSENRIQGSGLVLDWSQEKVTAVKASLPFALTQAQEKSLQEILTDMKSDHHMNR
                     LLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFESLQNLFPNLKLALLT
                     GSLKAAEKREVLETIAKGEADLIIGTHALIQDGVEYARLGLIIIDEQHRFGVGQRRIL
                     REKGDNPDVLMMTATPIPRTLAITAFGDMDVSIIDQMPAGRKPIVTRWIKHEQLPQVL
                     TWLEGEIQKGSQAYVISPLIEESEALDLKNAIALSEELTAHFAGKAEVALLHGRMKSD
                     EKDQIMQDFKERKTDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLRGRVGRG
                     DKQSYAVLVANPKTDSGKDRMRIMTETTNGFVLAEEDLKMRGSGEIFGTRQSGLPEFQ
                     VADIIEDFPILEEARKVASYISSIEAWQEDPEWRMIALHLEKKEHLD"
     gene            74796..75776
                     /locus_tag="ACL73L_000077"
     CDS             74796..75776
                     /locus_tag="ACL73L_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HGS0771135.1"
                     /translation="MKNPALLEEIKTYRGRDEVPEDFDAFWDGEVKNVSTLPSYHLEE
                     RDFHIPQVKCYELTFEGSKEGKVYARIVLPKSEEKVPLIFHFHGYMGRGWDWADMLGF
                     TVAGYGVVSMDVRGQSGYSQDGLRSPLGNTVKGHIIRGAMEGRDHLFYKDVYLDIYQL
                     VEIVASLSQVDEKRLSSYGASQGGALALVAAALNPRIQKTVAIYPFLSDFRRVIEIGN
                     TSEAYDELFRYFKFHDPFHETEEEIMATLAYIDVKNLAHRIQGEVKMITGLDDDVCYP
                     ITQFAIYNRLTCDKTYRIMPEYAHEAMNVFVNDQVYNWLCGSEIPFKYLK"
     gene            76626..76853
                     /locus_tag="ACL73L_000078"
     CDS             76626..76853
                     /locus_tag="ACL73L_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771136.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTVFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
     gene            77141..80038
                     /gene="nanA"
                     /locus_tag="ACL73L_000079"
     CDS             77141..80038
                     /gene="nanA"
                     /locus_tag="ACL73L_000079"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored neuraminidase NanA"
                     /protein_id="HGS0771137.1"
                     /translation="MNRSVQERKCRYSIRKLSVGAVSMIVGAVVFGTSPVLAQEGASE
                     QPLANETQLSGESSTLTDTEKSQPSSETELSGNNQEQERKDKQEEKIPRDYYARDLEN
                     VETVIEKEDVETNASNGQRVDLSSELDKLKKLENATVHMEFKPDAKAPAFYNLFSVSS
                     ATKKDEYFTMAVYNNTATLEGRGSDGKQFYNNYNDAPLKVKPGQWNSVTFTVEKPTAE
                     LPNGRVRLYVNGVLSRTSLKSGNFIKDMPDVTHVQIGATKRANNTVWGSNLQIRNLTV
                     YNRALTPEEVQKRSQLFKRSDLEKKLPEGAALTEKTDIFESGRNGKPNKDGIKSYRIP
                     ALLKTDKGTLIAGADERRLHSSDWGDIGMVIRRSEDNGKTWGDRVTITNLRDNPKASD
                     PSIGSPVNIDMVLVQDPETKRIFSIYDMFPEGKGIFGMSSQKEEAYKKIDGKTYQILY
                     REGEKGAYTIRENGTVYTPDGKATDYRVVVDPVKPAYSDKGDLYKGNQLLGNIYFTTN
                     KTSPFRIAKDSYLWMSYSDDDGKTWSAPQDITPMVKADWMKFLGVGPGTGIVLRNGPH
                     KGRILIPVYTTNNVSHLNGSQSSRVIYSDDHGKTWHAGEAVNDNRQVDGQKIHSSTMN
                     NKRAQNTESTVVQLNNGDVKLFMRGLTGDLQVATSKDGGVTWEKDIKRYPQVKDVYVQ
                     MSAIHTMHNGKEYIILSNAGGPNRENGMVHLARVEENGELTWLKHNPIQKGEFAYNSL
                     QELGNGEYGILYEHTEKGQNAYTLSFRKFNWEFLSKDLISPTEAKVKRTREMGKGEMG
                     KGVIGLEFDSEVLVNKAPTLQLANGKTATFLTQYDSKTLLFAVDKEDIGQEIIGIAKG
                     SIESMHNLPVNLAGARVPGGVNGSKAAVHEVPEFTGGVNGTEPAVHEIAEYKGSDSLV
                     TLTTKEDYTYKAPLAQQALPETGNKESDLLASLGLTAFFLGLFTLGKKREQ"
BASE COUNT        24208 a        14505 c        17989 g        23487 t
ORIGIN      
        1 tttatgtatt taatgatgat attgcgtcag agtggttttt gatgatgaat aagcgattga
       61 agactataca atctgaaatc gttaatgtaa agattgtaga tcatgttctt aaaaagtttc
      121 atttaccgtt aaagaattta agttatgcca ctttctttcg ttattttata cctaattttg
      181 tcaaagaaag tcgtgcttta tacctagatt ctgacatcat tgttacagga agtttagact
      241 atttatttga tatagaacta gatggttatg ccttggcagc agtagaagat tcttttggtg
      301 atgttccttc taccaatttt aactccggaa tgttattagt taatgtagat acttggagag
      361 atgaagatgc ttgttcgaaa ctgttagaac tgaccaatca atatcatgaa acagcatatg
      421 gagatcaagg aattttaaat atgttattcc atgatagatg gaaaagatta gaccgaaatt
      481 ttaattttat ggtggggatg gatagcgtcg cacacataga aggaaatcat aaatggtatg
      541 agatttctga gttgaaaaat ggagatttac ctagtgttat acattatact ggggtaaaac
      601 cttgggaaat aatttccaat aatcgcttta gagaagtttg gtggttttat aatctgttag
      661 aatggtctga tattttattg agaaaagaca ttattagtcg tagtttcgaa gaacttgtat
      721 acagtcctaa agctcataca gcaattttta cagctagttg tgagatggag catgtagaat
      781 atttgataga aaatttacca gaggtacatt tttctatact agcacataca tattttgcgt
      841 ctagtgtcgt tgctttatta agatatagca atgttacgat ttatccttgt ttttctccat
      901 ttgattatcg aaaaattttg gataatttag atttttattt agatattaat cattataaag
      961 aagtggataa tattgtatcc gttgttcaac aactatctaa accaattttt acctttgaaa
     1021 atactagtca tgatataggc aatcaaacta atatattttc ttcaaccgaa ccaaacaaaa
     1081 tggtagaggc tattagacaa tttataggag aataagttta tggcagacga actaattagt
     1141 attgtagttc caatctacaa tgttgagaat tatttgcgaa tgtgtttgga tagcattcag
     1201 aatcagacgt atcaaaattt tgagtgttta ttaatcaatg atggctctcc agatcattca
     1261 tccaaaatat gtgaagaatt tgtagagaaa gattctcgtt tcaaatattt tgagaaagca
     1321 aacggcggtc tttcatcagc tcgtaaccta ggtattgaat gttcgggggg ggggcgtaca
     1381 ttacttttgt agactctgat gattggttgg aacatgatgc tttagaccga ttatatggtg
     1441 ctttgaaaaa ggaaaacgca gatattagta tcgggcgtta taattcttat gatgaaacac
     1501 gctatgtgta tatgacttat gttacggatc cagatgattc tctggaagtg atagaaggta
     1561 aagcaatcat ggatagggaa ggtgtcgaag aagtcagaaa tgggaactgg actgtagctg
     1621 tcttgaagtt attcaagaga gagttactac aagatttacc atttcctata ggaaaaattg
     1681 cagaggatac ttactggaca tggaaggtac ttctaagagc ttcgaggata gtctatttga
     1741 atcgttgtgt ttactggtac cgtgttggtt tatctgatac tttatcgaat acatggagtg
     1801 aaaagcgtat gtatgatgaa attggggcta gggaagaaaa gatagctatt ttagcaagtt
     1861 cagactatga cttgaccaat catattttga tttataaaaa tagattacaa agagtgatag
     1921 caaaattaga agaacaaaat atgcagttca cagagattta tagaagaatg atggaaaaat
     1981 tgtctttact tccgtagata gtaataaaaa atgagatagc gtaatatgaa attacattta
     2041 acaaatttat acggcatggc tggtgatagt acggttatct tagctcaaaa tgctgttcaa
     2101 aagatagcta gtcaactggg atttagagag gttggtattt atttttacaa cattgcttca
     2161 gatagtcctt ctgaaatgaa taagcgtctg gatggtatta tggccagtat ctctattggg
     2221 gatattttag tctttcagtc tccaacctgg aatggctttg aatttgatcg tctcttgttt
     2281 gacaagctaa aggatatgca ggtgaaaatt atttgtttta tccatgatgt tgttcccctc
     2341 atgtttgata gtaactatta tctcatgaaa gactatatgt acatgtataa tctatcagat
     2401 gttttgatag tgccatcaga gagaatgaaa acacgcctga tggaagaagg attgacgact
     2461 aagaagattc ttgttcaagg gatgtgggat catcctcatg atttatcctt atacacccct
     2521 gcttttaaaa aagaactttt ttttgctgga agtttagagc gttttccaga cttacaaaat
     2581 tggtctcaag atacgccttt gagagtattt tcaaataaag gggaagctag ttctagtgct
     2641 agaaatctca gcatcgaagg atggaaaaaa gatgaggaat tgttgctaga attatcaaag
     2701 ggtggatttg gccttgtctg gggaacccat caaaatgagg gagaaagtaa ccaatactat
     2761 accttgaata tatctcataa ggtgagtacc tatctgacag cgggcatccc agtcattgta
     2821 ccaagtagct tgtcaactgc tgaatttata gtagatcaag gcttaggctt tgtggcgaat
     2881 agtctagagg aagttcatga gatagttgat aaaatgaatc tacaagaata tcaagaaatg
     2941 acgaatcgga taaaaacctt tagttatttg ctaaaagaag gttattttac taaaaaatta
     3001 tttgtagatg caatttacca tttgggagtt gaataagaga acaagatgat taaaacaatt
     3061 gtattagctg gtgatcgtaa ttacatcagg cagttagaaa caaccataaa atctatttta
     3121 taccacaatc gagatgttaa gatttatatt ttaaatcaag atattatgcc agattggttt
     3181 cgcaaaccac gaaaaatagc tcgcatgtta ggtagtgaga ttatcgatgt taaactacct
     3241 gaacaaactg tgtttcaaga ttgggaaaag caagatcaca ttagtagcat tacctatgct
     3301 agatatttta ttgcagatta tatccaagaa gataaggttt tatatttaga cagtgatttg
     3361 attgtaaata cttctttaga gaaattattt agtatttgtt tagaagaaaa atcactcgca
     3421 gcagttaaag atacagatgg aattacattt aatgcaggtg ttttattaat caacaataaa
     3481 aaatggcgtc aagagaaatt aaaagaacga ctaattgaac agagcattgt tacaatgaag
     3541 gaagttgaag aaggccgttt cgagcatttt aatggtgatc aaacgatttt taatcaggtc
     3601 ttgcaagatg attggttaga actaggtaga gcttataatt tacaagtagg acatgatgtg
     3661 gttgctttgt ataataattg gcaggaacat ctggctttta acgataaacc agcggtgatt
     3721 cattttacga cctacagaaa gccatggact accttgatag ccaatcgtta ccgtgattta
     3781 tggtgggaat ttcatgattt ggagtggaat caaattttac aacaccatat aggagagttt
     3841 gaaatgacat cgcctctaga taaggaattt tcttgtttaa ctttgacaaa ttcccaagat
     3901 ttagaaggaa tagaagagct agttacagct ctacctgagg tggtatttca tatcgcagct
     3961 tggacggata tgggagataa attaaaaaaa ttagctgtat ataataatgt gagattgcat
     4021 ccacaaattg ttcctcctgt attagataag ctgaaaaagt caacaaatct atatttggat
     4081 atcaatcatg gtagtgcaga tgagaacttt ttaaaatctt tgcaagaaca agaaaaaacg
     4141 ctactagctt ttcaatcgac tcagcacgga gagttaggac aaatcgtttt cgaaaatggg
     4201 aaagtttcct ttatgattga tacgattaaa gattttaaga aaaacggaca tcttacctgt
     4261 tttcgacaac ttccaagttt aacttgttta acgtttacgg cttctcagga tatcgaacaa
     4321 ttggattact tggctggaca gttgccaaat gttgtttttc aagttgctgc ttggacagct
     4381 atggggccaa aattatatga tttgtctaat cgttatccta atattcagct ctatccggca
     4441 atttctagag ataagctaga cgagttgaag gagaagatgg atgcttattt agatatcaac
     4501 ctactgactt caacatccga tatcgttgca gaaatggctc atctatctaa acctatacta
     4561 gccttttata aatctcaaaa tgggaataat ggccaaaggt tgtattcaag tgaacatcct
     4621 gaacgaatgt tggctgattt gcaaaaattg ataactaagg atatgctaga aaaaccgctt
     4681 gatataatcc aggtgaaagg gatagatgaa accttggatt atattattga acacaactct
     4741 tctttagttc gttttggaga cggagaagtc aatttgatgt ggggtttgcc tataccttat
     4801 caaaatcatg atctagagtt agctaaccaa ttaaaacata ttgtgggttt tgaaagtgac
     4861 gagaaattag tggtttgttt acctgatgct tttgacgatc gttttgtctt tacttggtgg
     4921 gctacaccat tctggaaaga acatatgaat gtttatatgg atttttataa agagttgtgt
     4981 aagggttcat ggtatggctc cacctttatc tcgcgtccat acattgatta tgaagataag
     5041 agtaaggcaa aaggacaatt tgaaaagcta aagagtattt gggagaatag ggatattctg
     5101 attgtagagg ggattacatc tcgctctggg gttggaaatg atttatttga taaggtcaaa
     5161 tctgtgaaaa ggattatttg tccatctcat aatgcttatt ccgttgtcga taacattcaa
     5221 gaggaaatta tgaaacatgc tgaaggaaga ctgattttat gtatgctggg tcctacagca
     5281 aaagttctga gttataatct atgccagatg ggctatcaag ttttggatgt aggccatgtt
     5341 gactcagagt atgaatggat gaaaatggga gctaaaacta aggttaaatt ttctcataaa
     5401 catactgcag aacataattt cgaccaagat attgaattta ttgatgatga aacctataac
     5461 agtcagattg ttgcacgaat attaaactag actatttaaa ataaatgata aggatttaaa
     5521 atgagaaata ccaaacgcgc tgtagtattt gcaggtgatt acgcttatat tcgacaaatc
     5581 gaaacggcga tgaagtcact ctgtagacac aatagtcatt tgaaaattta tctgctaaat
     5641 caggacattc ctcaggaatg gtttagtcaa ataagaatat atttacaaga gatggggggc
     5701 gacttgattg actgcaagtt aattggctca cagtttcaaa tgaattggtc taataaatta
     5761 cctcatatca atcatatgac atttgcacgc tattttattc cagattttgt aacagaagat
     5821 aaagttctct atctagatag tgatttgatt gtgactggtg atttgaccga tttgtttgaa
     5881 ttagacttag gtgaaaatta tttggcagca gctcgttctt gctttggagc aggagtcggc
     5941 ttcaatgctg gtgttctctt gattaacaac aaaaaatggg gatctgaaac tattcgacaa
     6001 aaattgattg acttaacaga aaaagaacat gagaatgtgg aagaaggaga ccagtcaatt
     6061 ttgaatatgt tgtttaaaga tcaatatagt tcccttgaag atcaatataa ttttcaaata
     6121 ggatatgatt atggggcggc agcctttaaa catcaattca tttttgatat tccgctcgaa
     6181 ccactgccac taattttaca ctatatttct caggataagc cttggaatca attttctgtt
     6241 ggacgtctaa gagaagtttg gtgggaatac tctttgatgg attggtctgt tattttaaat
     6301 gaatggtttt caaagagtgt gaagtaccct agtaaatcac aaatatttaa gttgcaatgt
     6361 gttaatttaa cgaattcttg gtgtgtcgag aaaatcgatt atttggcgga gcaattgcca
     6421 gaagttcatt ttcatattgt tgcttataca aatatggcaa atgaactact agctttaacg
     6481 cgttttccta atgttaccgt atatccaaat tccttaccaa tgttattgga acaaatagta
     6541 atagcttcag atttgtattt ggatttgaat catgatcgaa aattagaaga tgcatatgag
     6601 tttgtgctta agtacaaaaa accaatgata gctttcgaca atacttgctc tgaaaatctt
     6661 tctgagattt catatgaagg tatctatcca agctccattc cgaaaaaaat ggttgcagca
     6721 atcagatctt acatgaggta gagaacagta tgagaaaatc aatagtatta gcggcagata
     6781 atgcctatct tattccttta gagacgacta taaagtctgt attgtatcac aatagagatg
     6841 ttgattttta tattctcaac agtgatatag ctcctgaatg gtttaaatta ttggggagaa
     6901 aaacggaagt tgtgaattct acaattcgca gtgtacacat tgataaagaa ctttttgaaa
     6961 gctataaaac aggacctcat ataaattatg cttcttactt tagatttttt gtgacagaag
     7021 tggttgaatc tgatagggta ttgtatctgg attccgatat cattgtaact ggggaactag
     7081 ctactttgtt tgagatagat ctcaaaggat attcaattgg tgctgttgat gatgtctatg
     7141 cctatgaagg acgaaaatct ggatttaatg ctggtatgtt actaatggat gttgtaaaat
     7201 ggaaagaaca ttctattgtc aatagtttat tggaattagc ggccgagcag aatcaagttg
     7261 ttcatcttgg ggatcagagt attttaaata tttattttga ggataattgg ctagcgttag
     7321 ataaaacata taattatatg gtgggtgttg atatttatca ccttgctcaa gaatgtgaac
     7381 gtctagatga caatctacct acaattgttc actatgctag tcatgataaa ccttggaata
     7441 catatagtat atctagacta cgtgaattat ggtgggttta tagagatttg gattggtcag
     7501 agattgcttt tcaacgttcc gatttaaatt attttgaaag aagcaatcag tctaaaaaac
     7561 aagtgatgtt tgtgacatgg agtgcagata taaaacattt agagtattta gtacaacggt
     7621 tacctgattg gcattttcat ttggctgcac cgtgtgattg ttctgaggag ctgatctctc
     7681 tatcacagta tacgaatgta acagtatatc aaaatgtatt acatagtaga attgattggc
     7741 tattggacga ttctatagtt tatttagata ttaatacagg tggagaggtt tttaatgtag
     7801 ttacaagggc acaagaaagt ggcaagaaaa tcttcgcttt tgatatcaca cgtaaaagta
     7861 tggatgatgg actctatgac ggtatttttt ctgtggagag accagatgat ttagtggata
     7921 gaatgaagaa tatagagata gagtaatgag tgaattaatt agtgttgtgg taccgatata
     7981 caatacggga aaatatttag tggggtgtgt cgagcatatt ctgaagcaaa cctatcaaaa
     8041 tatagaaatt attttagttg atgacggttc tacggataat tctggggaaa tttgtgatgc
     8101 ttttatgatg caagataatc gtgtgcgagt attgcatcaa gaaaataagg gggcagcaca
     8161 agctaaaaat atggggatta gtgtagctaa gggagagtac atcacgattg ttgattcaga
     8221 tgatatcgta aaagaaaata tgattgaaac tctttatcag caagtccaag aaaaggatgc
     8281 agatgttgtt atagggaatt actataatta tgacgaaagt gacgggaatt tttattttta
     8341 tgtaacaggg caagattttt gcgtcgaaga attagctata caagaaatta tgaaccgtca
     8401 agcaggagat tggaaattca atagctcggc ctttatattg ccgacattta agttgattaa
     8461 aaaagaatta ttcaatgaag ttcacttttc aaatggtcgc cgctttgatg atgaagcaac
     8521 tatgcatcgc ttttatcttt tagcctctaa aatcgtcttt ataaacgata atctctatct
     8581 gtatagaaga cgttcaggaa gcatcatgag aacggaattt gatctttcct gggcaagaga
     8641 tattgttgaa gtgttttcta agaaaatatc ggattgtgtc ttggctggtt tggatgtctc
     8701 cgttctgcgt attcgatttg tcaatctttt aaaagattat aagcaaactt tagaatacca
     8761 tcaattaaca gatactgagg aatataaaga tatttgtttc agattaaagt tgttttttga
     8821 tgcagaacaa agaaatggta aaagttgaaa taaaagaatt gttatttacc atatcacaaa
     8881 caatgaaggt gaggggagtg ttttatgact aagatttatt cgtcaatagc agtaaaaaaa
     8941 ggactattta ccttatttct actgtttatc tatgtattgg gaagtcgtat tactctccct
     9001 tttgttgacc taaatactaa agatttttta ggaggttcaa cagcctatct agccttctca
     9061 gccgccctaa caggtggaaa tctaagaagt ttgtcaattt tttctgttgg attgtcccct
     9121 tggatgtcag ctatgatttt atggcagatg ttttcttttt ctaaacggtt gggtttaaca
     9181 tctacgtcta tagaaataca agatcgccgt aaaatgtacc tgaccttgat gatagctgtc
     9241 attcaatcat tggcagttag cttgagactg ccagtacaat cctcctattc tgcaatattg
     9301 gttgttctaa tgaatacaat attgctgata gcaggaacat tttttcttgt ttggttgtca
     9361 gatttaaatg cgagtatggg gattggaggt tctatagtca ttctcctatc cagtatggtt
     9421 ttaaatattc ctcaggatgt tttggaaaca tttcagacag tacacattcc aacagggatt
     9481 attgtgttac ttgctttatt aacccttgtc ttttcttatt tacttgccct tatgtatcga
     9541 gctcgctatt tggttcctgt taataaaatt ggcttacaca atcgatttaa acgctattct
     9601 tatctcgaaa tcatgttgaa tcctgcaggt gggatgcctt atatgtatgt gatgagtttt
     9661 cttagtgtac cagcttattt gttcatcttg ttgggattta ttttccctaa tcattcaggg
     9721 ttagcggctt tatcaaagga atttatgatt ggaaagcctt tgtgggtcta tgtttatatt
     9781 tcggtcttat ttttatttag tatcattttt gcttttgtta cgatgaatgg agaagagatt
     9841 gcagaccgta tgaaaaaatc tggagaatac atttatggta tttatccagg tgcggatact
     9901 agtcgattta ttaatcgatt ggtccttcgt ttctcagtca taggtggtct ctttaatgtg
     9961 gttatggcag gtggtcccat gctttttgtt ttgtttgatg aaaagttatt acgattggca
    10021 atgattccag gtttgtttat gatgtttgga ggcatgattt ttacgattag agacgaggtc
    10081 aaggctttaa ggctaaatga gacctataga cctttgattt aggagacttt tatgtattat
    10141 tttattccag cttggtatgg gtcagaaaga acatggcatg cagatatcac tccatggtat
    10201 ttttctcatt ttcgtctaga gtttgatgat acctttcacc agattcggct ctttcaagag
    10261 caagatatag attctcgtct attagtatta gcttaccagc ctcatctacg ttatttttta
    10321 tatagacatg gtgtgttaga aacggatact tattccgttt ttgatgttat gcaagatttt
    10381 cataatctcc atacccaagt tttaagcatt agagatattg agtgggatga tgactgtgaa
    10441 tttatttata gtccctttac gattatcgtt caaaaaaatg ggaagaaatt tgctaaggtt
    10501 gaacatggag ttgaaggctt catcagtgat atacagtatt ttggaccgaa tggtcaaata
    10561 catatgcact atatcatgga tgatcgaggg tttatatcga gtattatttt ttttgaagat
    10621 gggcaagcag cctatcaaga atatttgaat cctaagggag tttggcaatt tagagagcgt
    10681 ttaaaagaag gaggacaggt agaagtcaat ccaattttgg gttatcgctt taaaatgctt
    10741 acctatcaaa atatgggaga tctggtggca gaattttttg agaattatct gcaaacgtat
    10801 gtgaaggatc aggatatttt tatgcttcct tctcattctc atcatgacca gttggtacta
    10861 gatcgtttac ctagtactaa tcctaaactg ttgagtctgt tcattggacg taatcctcaa
    10921 gataccttta gggatttaga tgtaactttt gaaaaatcgg atttgatttt ggtggataga
    10981 gaggatagtt tacgattgtt gcaggagttg tatcctgaac gaatgcatca atgttatcat
    11041 ttatcatctt ttgacacccg attacgattg ggacgaagcc aaactaagaa agaatccatc
    11101 atttattttc aactggattt tgagcagggg attgataatc aagctctgct tcaagtcttg
    11161 tcctttgtcg ctgaaaataa ggatactgag gtgatttttg gagcctttgc tgctagtcag
    11221 gagcaaatga atgaggttga agggattgtt gagtctttca tccaagaaaa cattcaatcc
    11281 gaaaatctgg gaaaggcgat tgattatggt gatgcagaaa atcctctgga agaaaatcaa
    11341 caccaggact tacgcttaca gtttgttaac ttgaatgatg agttagattt gataaaaaca
    11401 ctagaatttg tccgtttgat tgtggattta aatagacatc ctcatctcta cacacagatt
    11461 gctgggatta gtgcaggaat tcctcaaatc aacctagttg aaaccgtcta tgttgaacat
    11521 ttaaaaaatg gttatttgtt agcagatgtt acagaatttt ctaaggctgc acattattac
    11581 acagataggt tgaaggagtg gaatgagtcc ttgatatatt caattgataa gattaaggag
    11641 cacacaggac aacaatttct tggaaaatta gagaaatgga tagaggaggt taaaaatgtc
    11701 aaaggaactt aatattttac agataggact tgccaattgg gaaaatcact atgacatacc
    11761 tgaaaatatg agttggtatt atttttaccc aaactcatca aaagcccttc gtgaaataat
    11821 tgaaaaagag gatattaacc gttttcatgc agttttaata gaagatggtc agtattccag
    11881 agacttattt tcctatgtaa aatattttga accttatact ttgttctata accagaatct
    11941 acaaataaat gatagagagg ttgtggattt tctaaaaaaa cgatgtgcac aagcaattga
    12001 ttttttaagt ccccaacaac taatcaatga tttaagtaaa tctctttttg gcggtgggta
    12061 tggtgataaa ctctttcctc cgacgataca agtcaatcca aattttacag gagctatttc
    12121 ttatcaagga ttggattatg tcagtttgga aggtgagttt gggcaagatt ttgcccagct
    12181 tgcctattgg gcttataata ttatggtgca aaaaacactc cctattgagt tgtggcttga
    12241 atatgagaag gaaggcaatt gtgactttcg tttggttatc cgtaaaatgt ggagtgggtc
    12301 tgttgatgat ttctttgaag aagtaatagt atctgaaaaa gacttggagc aagcactttt
    12361 tatggatagt cgagacggag actactttct ctcgatatct gttgaagcaa gaggtcgtgg
    12421 aactatcaaa ctaggtaatc ttcatcaacg atggagtcga aaacaatttg gtaagtttgt
    12481 acttggtgga aatatcctac atgattccaa gcgtgatgaa ataaactatt tcttccatcc
    12541 aggtgatttt aaaccgcctt tgactgtcta ttttgcaggt tatcgacctg cagaaggatt
    12601 cgagggttac tttatgatga aaactcttgg atgtcccttc attttatttt ctgatccacg
    12661 tttagagggg ggagcttttt atctcggaac ggatgagcta gagggaaaag taaaggatac
    12721 gatcactcac tatcttgatt atttaggctt tgatcataag gatttgattt tatcaggtct
    12781 ttctatggga acgtttccgg ctctctatta tggtgcttct tttgaacccc atgccatcat
    12841 agttggtaag cccttggcta atttaggaac tatagctagt cgtggacgtt tggacgcacc
    12901 gggtgtctct aacttagctt ttgattgttt aattcatcat acaggtggga caagttctca
    12961 agatatgacg gagttggatc agcgtttttg gaaaattttt aaacaagcaa atttttcaaa
    13021 gacaaccttt ggtttatcct atatgaaaga tgaagaaatg gatccacaag cctatgaaca
    13081 attagtgtct tatctgtgta atacaggtgc gaagatttta tctaaaggaa ctgctggacg
    13141 acacaatgat gatacagata ccaatatttc ttggtttttg cacttttata gaatggtctt
    13201 agagactggt tttggaaggg agaaaagatg attattacac agagacagtc tatttattgg
    13261 ggagaagttg gtgggactta tatgtatgga acaactgtat cttattaccc tgacaaaagt
    13321 gttcgtctgt ataatcctct attgccatct ggtgagattc taaagacttg gttttctagt
    13381 gtcaattacc aggctgcacg aacccaacct cagcttccct tattaaaaag aaagcaggag
    13441 tatcaactat cactggtttt tgattgccag cctgaaaatg gagtttatac caagataact
    13501 ttttttgacc gctatggtga tattttagaa aaaaaggtag aaaaagcgaa agatttcata
    13561 tttacttatc cagaagatag ttatacttat caagtttctc ttttaagtgc tggatttgag
    13621 tccttaactt tttatcattt ttctatcaag gagatcagaa gtgtttagac gtttaggtca
    13681 agatttccag cttaggaaag tgaaaaagat tttaaagcag attaatgccc tgaaaggcaa
    13741 gatgtcctct ctttcggatc aagaattagt agctaaaaca gtagagtttc gtcagcgtct
    13801 ttccgaggga gaaagtctag acgatatttt ggttgaagct tttgctgtgg tacgtgaagc
    13861 agataagcgg attttaggga tgttccccta tgatgttcaa gtcatgggag ctattgtcat
    13921 gcactatgga aatgttgctg agatgaatac gggggaaggt aagaccttga cagctaccat
    13981 gcctgtctat ttgaacgctt tttcaggaga aggagtgatg gttgtgactc ctaatgagta
    14041 tttatcaaag cgtgatgccg aggaaatggg ccaagtttat cgttttctag gattgaccat
    14101 tggtgtacca tttacggaag atccaaagaa ggaaatgaaa gctgaagaaa agaagcttat
    14161 ctatgcttcg gatatcatct acacaaccaa tagtaattta ggttttgatt atctaaatga
    14221 taacctagcc tcgaatgaag aaggtaagtt tttacgaccg tttaactatg tgattattga
    14281 tgaaattgat gatatcttgc ttgatagtgc acaaactcct ctgattattg cgggttctcc
    14341 tcgtgttcag tctaattact atgcgatcat tgatacactt gtaacaacct tggtcgaagg
    14401 agaggattat atctttaaag aggagaaaga ggaggtttgg ctcactacta agggggccaa
    14461 gtctgctgag aatttcctag ggattgataa tttatacaag gaagagcatg cgtcttttgc
    14521 tcgtcatttg gtttatgcga ttcgagctca taagctcttt actaaagata aggactatat
    14581 cattcgtgga aatgagatgg tactggttga taagggaaca gggcgtctaa tggaaatgac
    14641 taaacttcaa ggaggtctcc atcaggctat tgaagccaag gaacatgtca aattatctcc
    14701 tgagacgcgg gctatggcct cgatcaccta tcagagtctt tttaagatgt ttaataagat
    14761 atctggtatg acagggacag gtaaggtcgc ggaaaaagag tttattgaaa cttacaatat
    14821 gtctgtagta cgcattccaa ccaatcgtcc gagacaacgg attgactatc cagataatct
    14881 atatatcact ttacctgaaa aagtgtatgc atccttggag tacatcaagc aataccatgc
    14941 taagggaaat cccttactcg tttttgtagg ctcagttgaa atgtctcaac tctattcgtc
    15001 tctcttgttt cgtgaaggga ttgcccataa tgtcctaaat gctaataatg cggcgcgtga
    15061 ggctcagatt atctccgagt caggtcagat gggggctgtg acagtggcta cctctatggc
    15121 aggacgtggt acggatatca agcttggtaa aggagtcgca gagcttgggg gcttgattgt
    15181 tattgggact gagcggatgg aaagtcagcg gatcgaccta caaattcgtg gccgttctgg
    15241 tcgtcaggga gatcctggta tgagtaaatt ttttgtatcc ttagaggatg atgttatcaa
    15301 gaaatttggt ccatcttggg tgcataaaaa gtacaaagac tatcaggttc aagatatgac
    15361 tcaaccggaa gtattgaaag gtcgtaaata ccggaaacta gtcgaaaagg ctcagcatgc
    15421 cagtgatagt gctggacgtt cagcacgtcg tcagactctg gagtatgctg aaagtatgaa
    15481 tatacaacgg gatatagtct ataaagagag aaatcgtcta atagatggtt ctcgtgactt
    15541 agaggatgtt gttgtggata tcattgagag atatacagaa gaggtagcgg ctgatcacta
    15601 tgctagtcgt gaattattgt ttcactttat tgtgaccaat attagttttc atgttaaaga
    15661 ggttccagat tatatagatg taactgacaa aactgcagtt cgtagcttta tgaagcaggt
    15721 gattgataaa gaactttctg aaaagaaaga attacttaat caacatgact tatatgaaca
    15781 gtttttacga ctttcactgc ttaaagccat tgatgacaac tgggtagagc aggtagacta
    15841 tctacaacag ctatccatgg ctatcggtgg tcaatctgct agtcagaaaa atccaatcgt
    15901 agagtactat caagaagcct acgcgggctt tgaagctatg aaagaacaaa tccgtgcgga
    15961 tatggtgcgt aatctcctga tggggctggt tgaggtcact ccaaaaggtg aaatcgtgac
    16021 tcattttcca taagaggaga aaatatgaca atttacaata taaatttagg aattggttgg
    16081 gctagtagcg gtgttgaata cgctcaagcc tatcgtgctg gtgtttttcg gaaattaaat
    16141 ctgtcctcta agtttatctt tacagatatg attttagccg ataatattca gcacttaaca
    16201 gccaatattg gttttgatga taatcaggtt atctggcttt ataatcattt cacagatatc
    16261 aaaattgcac ctactagcgt gacagtggat gatgtcttgg cttactttgg tggtgaagaa
    16321 agtcacagag aaaaaaatgg caaggtttta cgtgtattct tttttgacca agataagttt
    16381 gtaacctgtt atttggttga tgagaacaag gacttggttc aacatgccga gtatgttttt
    16441 aagggaaacc tgattcggaa ggattacttt tcttatacgc gttattgtag cgagtatttt
    16501 gctcccaagg acaatgttgc agtcttatac caacgaactt tttataatga agacgggact
    16561 ccagtctatg atatcttgat gaatcaaggg aaggaagaag tttatcattt caaggataag
    16621 attttctatg gaaagcaagc ttttgtgcgt gcctttatga aatctttgaa tttgaataag
    16681 tctgatttgg tcattctcga tagggagaca ggcattggtc aggttgtgtt tgaggaagca
    16741 cagacagcac atttagcggt agttgttcat gcggagcatt atagtgaaaa tgctacaaat
    16801 gaggactaca tcctttggaa taactattat gactatcagt ttaccaatgc agataaggtt
    16861 gacttcttta tcgtgtctac tgatagacaa aatgaagttc tacaagagca atttgccaaa
    16921 tatactcagc atcagccaaa gattgttacc attcctgtag gcagtattga ttccttgaca
    16981 gattcaagtc aagggcgcaa accattttca ttgattacgg cttcacgtct tgccaaagaa
    17041 aagcacattg attggcttgt gaaagctgtg attgaagctc ataaggagtt accggaacta
    17101 acctttgata tctatggtag tggtggagaa gattctctgc ttagagacat tattgcaaat
    17161 catcaggcag aggactatat ccaactcaag gggcatgcgg aactttcgca gatttatagc
    17221 cagtatgagg tctacttaac ggcttctacc agcgaaggat ttggtctgac cttgatggaa
    17281 gctattggtt caggtctacc tctaattggt tttgatgtgc cttatggtaa tcagaccttt
    17341 atagaggatg ggcaaaatgg ttatttgatt ccaagttcat ctgaccatgt agaagaccaa
    17401 atcaagcaag cttatgccgc taagatttgt caattgtatc aagaaaatcg tttggaagct
    17461 atgcgtgcct attcttacca aattgcagaa ggcttcttga ccaaagaaat tttagaaaag
    17521 tggaagaaaa cagtagagga ggtgctccat gattgaactt tatgatcgtt atagtcatga
    17581 aagtcgagat ttacatgaaa gtctagtcgc tactggtctt tctcaacttg gagtggtcat
    17641 cgatgcagat ggttttctgc ctgatggtct gctttctcct tttacctatt atctaggtta
    17701 cgaggatgga aaacctctct attttaatca agttcccgtt tcagattttt gggaaatttt
    17761 aggagataat cagtctgctt gtattgaaga tgtgacgcag gagagggctg tcattcatta
    17821 tgctgatgga atgcaggctc gcttggttaa acaggtagac tggaaagacc tagaaggtcg
    17881 agtacgtcag gttgaccact ataatcgttt cggaacttgt tttgctaaaa ccacttatag
    17941 cgcagatagc gagccgatta tgacagttta ccaagatgtc aatggtcaac aagttttact
    18001 ggaaaaccat gtgacgggtg atatcttatt gactttgcct ggtcagtcca tgcgttactt
    18061 tgcaaataaa gttgaattta tcaccttctt tttgcaagat ttggaaatag acaccagtca
    18121 gcttatcttt aatactctag cgactccttt cttggtttcc tttcattatc cagataaatc
    18181 tggctcggat gtcttggtat ggcaggaacc tctttatgat gccattccag gtaatatgca
    18241 gttgattttg gaaagtgata atgtgcgtac taagaagatc atcattccaa ataagacgac
    18301 ttatgagcgc gctttagagt taactgacga gaaataccat gatcagtttg tgcacttggg
    18361 ttatcattac cagttcaaac gtgataattt cctaagacga gacgccttaa tcttgaccaa
    18421 ttcagatcag attgagcaag tagaagcaat cgtagaagcc ttgcctgatg ttaccttccg
    18481 tatcgcagca gtgacagaga tgtcttctaa gctcttagac atgctttgct atcctaatgt
    18541 ggccctttac cagaatgcta gtccacagaa gattcaggag ctgtatcaac tgtcggatat
    18601 ttacttggat ataaaccaca gtaatgagtt gctacaggca gtgcgtcagg cctttgagca
    18661 caatctcttg attcttggct ttaatcagac ggtgcacaat agactttatg tcgctccaga
    18721 ccatctattt gaaagtagtg aagttgctgc tttggttgag accattaaat tggccctttc
    18781 agatgttgat caaatgcgtc aggcacttgg caaacaaggc caacatgcaa attatgttga
    18841 cttggtgaga tatcaggaaa ccatgcaaac tgttttagga ggctaacatg tcagaggaag
    18901 atttatttta caaagacgtt gaaggccgca tggaagagtt gaaacaaaaa cccatcaaga
    18961 aggaaaaaga aacccgaggg gaaaagatta gtaagacttt ttcactttta ctgggtttga
    19021 tgattctgat tggtttgctc tttactttgc tgggaatttt gaggtagatc tatgattgac
    19081 atactaattg ttttagctat tatcctatct cttgctttga ttgtattggt aactatacaa
    19141 ccccgtcaaa atcaactatt ttccatggat gccactagta atattggtaa accaagctac
    19201 tggcagagca acaccttggt caaggtgctc actttattgg tgagtttggc tttatttgtt
    19261 ctactattaa cctttatggt gattacttat aaataaaaga aaacttcaga tattcacctt
    19321 ttgtggattg gtctgaagtt ttctttttta tactcaatga aaatcaaaga gcaaactagg
    19381 aagctagccg caggctgctc aaaacacggt tttgaggttg tggatagaac tgacgaagtc
    19441 agctcaaaac acggttttga ggttgtggat agaactgacg aagtcagtaa catatatacg
    19501 gcaaggtgaa gctgacgtag tttgaagagt attactgtct atatttttgg taaaaatcaa
    19561 cttttacttg gatgaaggtt ttggcttcac gtaggagttg aagaagggtg gcgcgggttt
    19621 caaattcttc tcttgtcttg ggcagactgc ggttccggaa gacttccaga taacgttcaa
    19681 tttcatctag caaatcagaa gcaggattgg tctggctcag ttgacctgca atttttgaaa
    19741 agagttgcgc taagatcagg ctttcactgg cggcaaggtg acaagtgtta atctgttggg
    19801 ccatgtttct caggatacga ctttgtcgct gtctcatctc aaagtagtgg atatggtagt
    19861 cggtctggtg aaagaggtgg tcagagtgat ccaaatagac cagtctgagg gcttctttca
    19921 aaagcgtgtc taattctgct accagctgtg ctcggttgcg tccgtctcct ctggataaat
    19981 agtatttgaa gcgctggagg atatctttta acttttcttc caccagcgtg tggtagtgct
    20041 ggatttcctc ttctcgtgaa ggcatataga gattaacaag caaggcaaat cctgtaccaa
    20101 tagcaaagag aaggaattcg ttgactagga ggtctggaga ggttgactct tgaaccaaga
    20161 gatggctaac caaaaccgtg cttggtgtga tgccaatttc ccagcccatc ttgtaggcta
    20221 aaggaacgta gaaggccaga tagaggccga gactccagat atgaaatccg ctcaagtgaa
    20281 aagctagaac accgatagcc agagctagaa gcatagaaaa aagacgattg cgagccagtt
    20341 ttaaagtact tctacgcgta tcagataggc tcaagagagc gataattcca gccgaaactg
    20401 ctgacgaaag attgagaaaa taagcaagca ggcaggcaag acaggtagct aagatgagct
    20461 tggtcgtacg ttggctaata gacataagaa tttcctaata agttagaata aaagcgtaaa
    20521 agacaagaca tgagcaggct tgccttgatg agttattttt tacgggttgc tgcgtattcg
    20581 gcaacggcgg taaagaggac atctgtagaa gagttaaggg ctgtttcaca tgagtcttgg
    20641 atgacaccaa tcacaaaacc aaccccaaca atttgtatgg caatatcgtt agaaataccg
    20701 aaaaggctac aagcaactgg gataagaagg agggaacctc cggcaatacc tgaagcacca
    20761 caggctgaga tagctgctac cacactgagg acaaaggctg tggcaaagtc aacaggaatt
    20821 ccaagagtgt taactgtaac aagggtcaaa aggttaatgg taatcgctac tccagccata
    20881 ttgatagtag aaccgagtgg gatagaaaca gaataggtat ctgggttgag tccaaggtca
    20941 tggcagagtt tcatattgac aggaatgtta gccgcagaac tacgagtgaa aaaggctgtc
    21001 acaccgctga cacggaggca gttccaaact agagggtaag gattgcgtct cataaagaag
    21061 aaggcaatca aagggttgac cacaggggca acaaaaagca tagtcgttac taatagaacc
    21121 aataaaatac cgtagttggc aaggcttccg actcccttgt cagaaatggt tttaaaaaca
    21181 agaccaagga ttccaaatgg agtcagattg atgatccatt cgacaagttt agaagtcacg
    21241 tcagcgatag tttttagcaa ttctttacta tttttactgg cttctctcat agcgattcca
    21301 aaaatgactg cccaagataa gattccaata tagttagcag taagcagggc gttgactggg
    21361 ctgtcaacca gtttgagcaa gaggttgctg aggacctgcc caatcccatc tggtggtgca
    21421 atttcagtat tggcactatt tagggtaatt tcaataggga cgatgaaact tgctagtaca
    21481 gctacaagag cagcggcgaa agtccctatc ataggatata caagaaaaca acagttttca
    21541 tattgctatc ttgtcccttt tgatgttggg aaagggcatt ggcaacgagg gcaaagacta
    21601 ggataggagc aacagctttt agacctccaa cgaataaatc cccgagtagc ccaatccctg
    21661 agagattagg aagggtcagt cctaggattc cccataaagc ataccaatca agatacgctt
    21721 gacaaggctt gccttattcc aagcatgaat gattcttttc ataataatct cctttttgtg
    21781 tagtgattat gattatagta taaatgatag acaaaatcaa gaattttctg tctatttttt
    21841 gaatatgtat ggagaatgag actgatgaaa atatggtata atgaaataaa ggagttttat
    21901 atgcaaaaat ttattcaggc ttatattgaa aagctagatg tgacaaccat tatcgagaat
    21961 attctaacca aggtcatttc tcttttactg cttttgattg tattttatat tgctaaaaaa
    22021 atgcttcata ccatggtgca gagaattgtc aaaccttctc taaaaatgtc tcgtcatgat
    22081 gtcggacgcc aaaaaaccat ctcacgttta ctagaaaatg tgtttaatta tacgctatat
    22141 ttctttttac tctactgcat tttgtcgatt ttaggtttgc cagtttctag tttgctggct
    22201 ggagctggta ttgctggggt agcgattggt atgggagccc aaggctttct atctgatgtc
    22261 atcaatggct ttttcatcct ctttgaacgt caactggatg tgggagatga ggtcgttctg
    22321 acaaatggac cgattactgt atcgggtaag gttgtcagtg tgggaattcg tacgacacag
    22381 cttcgtagcg aggagcaagc ccttcacttt gtccctaacc gaaatatcac agttgttagc
    22441 aatttctcac gcacagacta gacctgttat tttaagtaat ttgtggtaca atagagggag
    22501 tttaataagg agaaaagatg gttttagaaa agcagttggg caatggttgt acctggatag
    22561 acctagacct aggaaagttg aataaactag aagacctttc tgaaatttac ggtttggaca
    22621 aggaaaccat tgaatacgca ctggatagaa acgagcgcgc ccacatggac taccaccgtg
    22681 aaagtgggac ggttaccttt atctataatg tcctagacgt aaaaaaggac aaggcctact
    22741 atgagacttt tcccatgacc tttatcgtcg agcatcgtcg cctgattacc attagtaata
    22801 ccaagaacgc ctatgtcatt gaacagatga ctcgttatct ggagaaccat gacacgcttt
    22861 cgatttataa gtttctcttt gccagtctgg aaatcatcag caatgcctac tatcctgtca
    22921 ttgagcagat ggacaagagt agggatgagg tcaatgacct cttgcgccag cgaactacca
    22981 agaaaaacct ctttgccctg tctgatttgg agactggtat ggtttatctg acggcagctg
    23041 ccaaacaaaa tcggattttg ttagagcata ttcaaggtca tgccttgtat cgtagttttg
    23101 atgagattga gagagaacag tttgatgatg ccatgattga ggctcatcag ctggtatcca
    23161 tgacagacct aatctctcag attttacagc agctttcagc ctcttacaac aatattctaa
    23221 acaataatct gaatgacaat ttgacaacct tgactattat ttcagtcttg ctagctgttt
    23281 tggcagtcgt gacaggcttt ttcggaatga atgttccctt acctttaaca gatgagcccc
    23341 atgcttggct ctatattagt ttggctagtg caggtttgtg gattgtttta tccttgttac
    23401 taaggaaaat tgcgaaaaaa agttaagaaa aggagccaga atggcgattg aaaattatat
    23461 accagatttt gctgtggaag cagtctatga tctgacagtc ccaagcctgc aggcgcaggg
    23521 aatcaaggct gttttggtcg atttggataa taccctcatt gcttggaaca accctgatgg
    23581 aacgccagag atgaagcaat ggctacatga ccttcgggac gcgggtattg gcattatcgt
    23641 agtgtcaaat aacaccaaaa aacgcgttca acgagcagtt gagaaatttg ggattgatta
    23701 cgtttactgg gccttgaagc ccttcacatt tggtattgac cgtgctatga aggaattcca
    23761 ctatgacaaa aaggaagtgg tcatggttgg tgaccagctc atgacagata tacgagcagc
    23821 ccaccgtgca gggattcggt caattttagt caaacccttg gtccaacatg actcaatcaa
    23881 aacgcagatt aaccgaactc gtgagcgtcg tgttatgaga aaaatcactg aaaagtacgg
    23941 accgattaca tataaaaaag gaatttaact atggaagaaa ttctctgtat tggttgtgga
    24001 gcaaccattc agacgacaga taaggctggt cttggtttta ccccccagtc ggcacttgaa
    24061 aaaggtttgg agactggcga agtctattgc caacgctgtt tccgtctccg ccactacaat
    24121 gaaatcacag atgtccagtt gacgaacgat gatttcctca agctcttgca cgaggtggga
    24181 gacagtgatg ccttagtggt caatgtcatt gatatctttg attttaatgg ctctgtcatc
    24241 ccaggtttac cacgtttcgt ctcgggcaat gatgtcctct tggtaggaaa taaaaaagat
    24301 atcctcccta agtcagttaa gtctggtaag attagccagt ggctcatgaa acgtgcccat
    24361 gaagaaggtc ttcgtccagt cgatgtggtc ctaacttcag cacaaaataa acatgccatt
    24421 aaggaagtca ttgacaagat tgaacactac cgtaagggcc gcgatgtcta tgtggtcggt
    24481 gtgaccaacg ttggaaaatc aactctaatc aatgctatta tccaagaaat cacgggtgat
    24541 cagaatgtca tcactacttc acgcttccca gggacaacct tggacaaaat agagattccg
    24601 cttgacgacg gatcttatat ttacgatacg ccgggaatta tccaccgcca ccagatggct
    24661 cactacttga cggccaaaaa cctcaagtat gtcagtccta aaaaggaaat caagcctaag
    24721 acctatcagc ttaatcctga gcaaacccta tttttaggtg gtttgggacg ctttgacttt
    24781 atagcaggag aaaagcaagg atttactgct ttctttgata atgaactcaa actccatcgt
    24841 agcaagcttg aaggagctag tgctttctac gataagcacc tgggaactct tctgacacca
    24901 ccaaatagca aggaaaaaga agatttccca aggctagtcc agcatgtctt taccattaaa
    24961 gataagacag acctagtcat ctcaggccta ggctggattc gtgtaacagg catagcaaaa
    25021 gtcgccgtct gggcaccaga aggcgtcgcc gtcgtcacac gaaaagcaat tatttaagca
    25081 cagaaaggaa agggttgtct gaatttgggc gagcaaggcg agccccatag agaatacttt
    25141 tcgctgtggt gtaagttggt acaagtgatt gtaccaactg cggaaaattt gagaccttag
    25201 gctcaaattt tagtcatgaa agtccgaagg actttgctga cgtccgtcac cacttcagaa
    25261 aagtataaaa agaaactctt ttaaagaaat tatgtcatta acatcaaaac aacgtgcctt
    25321 cctcaacagc caggcacaca ccctcaaacc tatcatccaa atcgggaaaa atggactcaa
    25381 cgaccaaatc aaaaccagcg tccgtcaagc tcttgatgcg cgtgaattaa tcaaggttac
    25441 tctcttacaa aacacagatg aaaacatcca cgaagtagct gaaattttgg aagaagaaat
    25501 cggtgtggat acagtccaaa aaataggacg catcttgatt ttgtttaaac aatctagcaa
    25561 gaaagaaaat cgcaagattt ctaagaaagt caaagaaatc taagaccgaa actccaaata
    25621 actgttttta tagagaaata aaggggacta gcctatgaca atcgaactat tgactccctt
    25681 taccaaggta gagttggagc cagaaatcaa ggagaaaaaa cgcaaacaag ttgggatttt
    25741 aggggggaat tttaaccctg ttcacaatgc ccatctcatt gttgcggatc aagtacggca
    25801 acagttggga ctggatcaag ttctgctcat gcctgaatac caacctcctc acgttgataa
    25861 aaaggaaacc atccctgaac accatcgtct caagatgctt gagttggcaa ttgagggaat
    25921 tgacggccta gtcattgaaa ccattgagtt ggagcgcaag ggtatttcct acacctacga
    25981 caccatgaag attttgacag agaagaatcc agatacggat tattacttta tcatcggtgc
    26041 cgacatggtt gactatctgc ctaagtggta ccgaattgat gaactggttg acatggttca
    26101 gtttgtgggg gttcagcgtc cacgctacaa ggcagggact tcctatccag ttatctgggt
    26161 ggacgtaccg ctcatggata tctcgtccag catggtgcgt gccttccttg cccaaggtcg
    26221 gaaacccaac tttctcctac ctcagcttgt gctagactat atcgagaagg aggggctcta
    26281 ctgatgtcct atcaagacta tatcaactgc tctcgtgagg ccttgttgga aaaaatggca
    26341 gagcttctac ctgagaaacg tctaacccat tgtctaggtg tggagcgtgc cgctatggaa
    26401 ttagctcagc gctttggtgt agatgttgag aaagcaagtc tagcaggtct tcttcatgac
    26461 tatgctaaaa agttgtcaga tcaggaattt ctagacttga ttgaccgtta ccagctagac
    26521 cctgacctca aaaactgggg caataatgtc tggcatggta tggtcggaat ttacaaaatt
    26581 caggaagatt tggatttgca tgattcagaa atcctgcgag ccattgaagt ccatacagtc
    26641 ggtgctggtc agatgacaga cctagataag gttgtctatg tagcagacta tatcgagcac
    26701 aatcgatcct ttcctggagt agatgtggcg cgtgagattg caagtctatc gctcaataag
    26761 gcggtggcct acgaaacagc tcgtaccgtg gagtatctag ctcatcaggg attccccatc
    26821 tatccccaaa cccttgaaac ctataacgcc tttgtgcact atttgaaaga ggactaaatg
    26881 aagacggctt ttataatcat tgatgttcag aatgttttag tagaaactgg gttccagaca
    26941 aaaagtctat tggaaaaaat ttcttattta caaaaccagg ctagaagcaa gaatattgaa
    27001 attatctatg ttcaacatat tgagaactct gaagctcaaa catcagaagg ttggcagtta
    27061 tctgcgcttt taaatcgaaa acctgctgaa aaggtctttc agaagaagta taacagtatt
    27121 ttcaaagaaa ctggcttaaa agaatacttg gataaacagg ggattgaaaa attagtttta
    27181 tgtggtatgc agacagaata ttgtgtggat acctctgtca aggttgcctt tgaatatggc
    27241 tatcagctta ttgttccaga aggtgctgtc acaacctttg atggggatga cattccagca
    27301 gaaacgatta atgaatttta tgaggacatt tgggaggagc gctttgcaga tgtcctagat
    27361 tacaaacata ttttctaaaa agaggattaa atgaacgaaa aagaattact agaactagtc
    27421 gtgaaagcgg ctgatgagaa acgtgcggag gatatcctcg cacttgatgt acaagatttg
    27481 actagtgtga cggactactt tgtcatcact agctcaatga atagccgtca gttggacgct
    27541 atcgcagcta atatccgtga aaaagtagct caagcaggct ttaaaggtag ccatgtcgaa
    27601 ggcgatgcag ctggaggctg ggtcttactg gacctcggtg ctgttgtcgt gcatatcttt
    27661 tcagaagaaa tgcgtgccca ttataatcta gagaagctat ggcatgaggc gaattcagta
    27721 gatatttcag aagctcttgc ttagaagatg aactcagttg tagtcaactg ggtttctttg
    27781 cttattttag aaaaattgat agaaaggtaa agggctagtg gtgtttacca tggagctctt
    27841 ggtatcatga ttatggcgac ttatgaaacc tttgcggcgg tctatgatgc ggtcatggac
    27901 gatagtttat acgacaaatg gacgaatttt tctctgcgtc atttgcctaa gaccaaggag
    27961 agaaagaaac tcttggaatt ggcttgtggg acaggaattc aatcagtgcg cttctctcag
    28021 gctggttttg atgtgactgg acttgacttg agtgcggata tgttgaagat tgcggagaag
    28081 agagcaactt cagccaagca aaagattgct tttatagaag gtaatatgct gaatttgtcc
    28141 aaggcaggga aatacgattt tgtcacgtgt tattcggact ctatctgcta tatgcaggat
    28201 gaggtggaag taggggacgt ctttaaggac gtgtacaatg tgctgaatga agagggagtt
    28261 ttcatctttg acgtgcactc gacctaccag acagatgaag tattccctgg ctattcctac
    28321 catgaaaatg cggaagattt tgccatgctt tgggatacat atgaggacga agttcctcac
    28381 tccatcgtgc atgagctgac tttctttatc aaggaggctg acggttcctt tagtcgccac
    28441 gatgaagtac atgaggagag gacctatgag atcttgacct atgatatctt gctggaacag
    28501 gctggattta agtccttcaa actctatgca gactttgagg ataaggaacc aacagaaact
    28561 agcacccgtt ggttttttgt ggcgcagaag taggagaaca tcatgaccat cacaggtatt
    28621 atcgcagagt tcaatccttt tcataatggc cataaatacc tgctggatca ggcagaggga
    28681 ctgaaaattg ttgccatgtc agggaatttc atgcagcgtg gagagcctgc tattgtggat
    28741 aagtggacaa gggcccagat ggcgctggaa aatggcgcag acttggtagt ggaattgccc
    28801 tttttagtca gtgttcaggc agctgatttc tttggtcaag gagctgtgga tatcttggat
    28861 cgcttgggca ttgatagtct agtttttggg acagaggaag ttcgagatta tcagaaaatc
    28921 gctgacttat acacagagaa aggtactgag atggagaaat ttgtggaaaa tctgcctgat
    28981 tccctttcct atccccagaa aacccaagct atgtggaagg aatttgctgg tcttgatttt
    29041 tcaggtaata cccccaatca tgttcttgct ctggcctatg caaaggcggt tgcaggacga
    29101 aatatcaagc tgcatccgat tcagcgtcag ggggcaggtt accattctgt gaataaagat
    29161 gtggactttg cctcggcgac agccctccgt caacatcaga aggaccaaga tttcttagaa
    29221 cgctttatgc cttctgttgc cctatttgag caggccagta aggtgatctg ggaagactat
    29281 tttcccttgc tccgctatca aatcttgtca aatcccgacc taaccaccat ctatcaggtc
    29341 aatcaagaaa tggctgtgcg cattaaagaa gctattaaaa cagcccagtc tgtggaagaa
    29401 ttggtcgagt tagtgactac caaacgttac accaaggcgc gtgtcagacg cctattgacc
    29461 tatattttga tgcaggctag agaaagtgac ttgccagaag ccattcatgt ccttggattt
    29521 actgaaaaag gtaggcaaca tctcaagtct ttgaaaggtc aggtcagtct agtcagccga
    29581 attggcaaag aaccttggga tgctatgact caaaaggctg accagattta ccaactagga
    29641 aagccaagta tagcacaaca gaattttgga agagtgccga ttagaataga aacaaactaa
    29701 gtctaccgaa aggtggactt tttgagattt tttacgaata atactcttcg aaaatcaaat
    29761 tcaaatcgcg tcaacgtcgc cttgccgtac tcaagtacag cctgcggcta gtttcctagt
    29821 ttgctctttg attttcattg agtataaata gatagaaaag aaaatcttgt acaagttcct
    29881 gacaatttct ttcttttcgt gtataatagt ggaaaagagc taaaacgagg tatggttatg
    29941 gaagcagtcc tttactcaac attccgaaat catttaaagg actacatgaa gaaggtaaat
    30001 gatgaatttg agcctttgac ggtggtcaat aaaaatccag atgaggacat tgtagtcctt
    30061 tcaaagagtg agtgggatag tatccaagaa accctgagaa ttgctcaaaa taaggaactt
    30121 tctgataagg ttttgcgagg aatggctcaa gttcgtgctg gaagtactca ggtccatgtt
    30181 attgaggagt aagaaatgct gctcaagttt acagaagatg cctgggcaga ttattgctac
    30241 tggcaaaacc aagataaaaa aacactaaaa agaatcaata aattaatcaa ggatattcaa
    30301 cgcgatccat ttacaggaat aggaaaacca gagccactca aatatgatta ccaaggagcc
    30361 tggtcacggc gtattgatgc agaaaatcgc ttgatttata tgatggatgg agatagcgtg
    30421 gctttcttgt cctttaaaga tcattactaa gtctactgca aagtaggctt tttctcaagt
    30481 ctctactaaa ctactatttc taaacaacaa gtttattttg gtaaatgtgt tccaaattcc
    30541 ccaaaacata ttctatttta ggtttgtgaa aatcactttt ttatggtaga atgtaaaaga
    30601 atgtatgtca ttcggaataa caataaaatg aggtaaaaac atggaaatca tgtcgcttgc
    30661 gattgctgtt tttgccgtca tcattggttt agtcattgga tatgtcagca tctcagctaa
    30721 gatgaaatca tctcaggaag ctgcagagtt gatgctttta aatgctgaac aagaagcaac
    30781 taatttacgt ggacaagctg agcgtgaagc ggatttactt gttaatgaag ccaaacgtga
    30841 aagcaagtct cttaaaaaag aagcactatt ggaggccaaa gaagaagcca gaaaataccg
    30901 tgaagaagtg gacgctgaat tcaaatcaga acgtcaagaa ctcaaacaaa tcgaaagtcg
    30961 tttgacagag agagctacta gccttgaccg taaggacgac aatttgacga gtaaagaaca
    31021 aacacttgaa caaaaagaac aaagtatttc tgatagagcg aaaaaccttg atgcgcgtga
    31081 agagcaatta gaggaagtcg aaagacaaaa agaagcagaa ctagagcgta ttggtgctct
    31141 gtctcaggca gaagcacgag atattatctt ggctcagaca gaggaaaact tgaccaggga
    31201 gattgccagt cgcattcgcg aagctgagca agaggtcaag gaacgttctg acaaaatggc
    31261 caaggacatc ttggttcaag ctatgcaacg tatcgctggt gaatatgtag cggagtcaac
    31321 aaactcaacg gttcatctgc cagacgatac tatgaaggga cgcattattg gtcgtgaagg
    31381 tcgtaacatt cgtacctttg aaagtttgac aggggtcgat gtgattatcg acgatacacc
    31441 agaagtggtg accttgtcag gatttgatcc gattcgtcgt gagattgccc gtatgactat
    31501 ggaaatgttg ctcaaagatg gtcgtataca tccagctcgt atcgaagagt tggttgagaa
    31561 aaaccgtcaa gagattgaca ataagattcg tgaatacggt gaggctgctg cctatgaaat
    31621 tggtgcgcca aaccttcatc cagacttgat gaagattatg ggacgtttgc agttccgtac
    31681 ttcatatgga caaaatgttt tgcgccattc gattgaggtt gctaagttgg ctggtatcat
    31741 ggcgagcgaa cttggtgaaa atgcggctct tgcccgtcgt gctggattcc ttcacgatat
    31801 cgggaaagcc attgaccatg aggttgaagg tagccacgtt gaaatcggta tggaattggc
    31861 ccgtaagtac aaggaacccc cagttgtggt gaatacgatt gctagtcacc acggagatgt
    31921 tgaagctgag agcgtgatag cagttatcgt cgctgcagca gatgccttga gcgcagcccg
    31981 tccaggtgct cgtagtgagt ctcttgaaag ctacatcaag cgtctccatg atttggaaga
    32041 aattgctaac ggctttgaag gagtgcaaac tagctttgcc cttcaagcag gacgtgaaat
    32101 tcgtatcatg gtcaatccag gaaaaatcaa ggacgacaaa gtcacaatct tggctcacaa
    32161 agttcgtaag aaaattgaaa acaatctcga ttatccagga aatatcaagg taaccgtgat
    32221 tcgcgagctt cgtgcagtag attatgctaa ataaataaga aagagcagtt gaaaaattac
    32281 tgcttttttg ttacactaga tagaaagact gtaaaggata atctggcttt ttgccattat
    32341 tctagagaaa tgttatttca cagattgact aaaaggagac acaatggcag accgaggctt
    32401 actaatcgtt ttttctggtc cttcaggggt tggaaaagga acggttagaa gagagatttt
    32461 tgagagttct gaaaaccaat ttcaatattc tgtatcgatg acgacacgcg cacaacgtcc
    32521 tggagaagtg gacggtgttg actacttctt ccgtactcgt gaagaatttg aagagctgat
    32581 tcgtcaagga cagatgttgg aatacgcaga atatgtcggc aactactatg gaactcctct
    32641 gacctatgtc aatgaaacct tggacaaggg aatcgatgtt ttccttgaaa ttgaagttca
    32701 gggtgctctt caggtcaaga aaaaggttcc agatgctgtc tttatcttcc tgacaccacc
    32761 agatttggat gaattgcaag atcgcttggt aggtcgtgga acagatagtg cagaagtgat
    32821 tgcccaacga atcgaaaagg ccaaggaaga aattgccctc atgcgtgagt atgattatgc
    32881 gattgtcaac gatcaggtgc ccctagctgc tgaacgtgtc aaatgtgtga ttgaagcaga
    32941 acacttctgt gtggatcgtg tcattggtca ctatcaggag atgttaccaa aatctccaac
    33001 tacccgataa aatttagaaa ataggtacaa gcaaatgatg ttaaaaccct ctattgatac
    33061 cttgctcgac aaggttcctt caaaatattc actcgtaatc ttggaagcaa aacgtgccca
    33121 cgaattggaa gcaggtgccc cagcaactca aggtttcaag tctgaaaaat caactcttcg
    33181 cgctttagaa gaaatcgaat caggaaacgt tacaattcac ccagatccag aaggaaaacg
    33241 tgaagcagtg cgtcgccgta tcgaagaaga aaaacgccgc aaagaagaag aagaaaagaa
    33301 aatcaaagag caaattgcta aagaaaaaga agatggtgaa aaaatttaag gttgggggga
    33361 ctcaatctta ttttttctat tgcaagaatg tactgacaag gaggaggtga gaagatggcc
    33421 ctagctaaga ttatcgtaga tgtgcccttg atgcagacgg accagcccta tagttacagg
    33481 attccggagg aatttgaggg aatgctggaa gttgggatgc gggttcatgt gccttttggt
    33541 aagggtaatc gcctgattca agggattgtt tttggtttgg agtcccaatc agatggagaa
    33601 gagatggagc aagatttaaa agatattgca gaggtgctgg atttttctcc tgttctcacg
    33661 ccagaacaac tctggctggc tgaggagtta cgcaaatctg tcttctccta caaaatctct
    33721 atcctcaagg ccatgcttcc gggatttctg aattctagct atgacaagat tctctatcct
    33781 ctggaaggct tgagtcagga agaacgagtg cgcctgtttg gttcagaaga ttcgctagcc
    33841 ttttcttctc tggatttagg taagcaagct gaaatgatgc gtttgactag aaaaggcctg
    33901 cttggtctgg aatatcaggc agtcgatcaa aagaaggtca agacccagtc ttggtatgag
    33961 gttgaccatg ctcaattaga aggtgttgag atttctacac gtgccaagaa aaagttggaa
    34021 ctgagagatt atctgctgtc tcatccagag agcgcttcct tggctagctt gttagagtcc
    34081 tactcgcgag agcaagtcaa cttctttgtg gatcaaggtg ctgttaccat agtccaaaag
    34141 gaagttcgac gctcggctgc ttattttgaa ggaattgaag caagtagacc tttggagtta
    34201 aatccagaac aaagacaggc gcgtgatgcg gttgtgagtt ctattggcag ttctcaacct
    34261 ccctttctac ttcaagggat tacaggaagt gggaagaccg aggtttactt gcagattatc
    34321 caaggtgccc tggataaggg caagacagct attttgctgg tacctgagat ttccttgact
    34381 cctcagatga ccgagcgttt tatagcacgt tttggggaca aggtagccat tcttcactca
    34441 ggattatcca atggcgaaaa gtatgatgaa tggcgcaagg tggagcgtgg cgatgcccaa
    34501 gttgttgttg gtgccagatc tgctatcttt gctcctctga aaaatctggg tgtcatgatt
    34561 attgatgaag agcatgaagc ggcttataag caggacagca atccccgtta ccatgccaga
    34621 gaggtagcta ttttacgggc tcagtataat caagcaactc tggtacttgg atctgcaacg
    34681 ccgagcttag agagccgggc acgtgctggc aaaggcgtct atcaacactt acgtctaacc
    34741 caacgtgcca atcctttggc tacaatccct gaggttcaag tgattgactt tcgagactat
    34801 atcggacaaa atgagacgtc aaactttacg cctcctttgc tagaggctat ccaagaccgt
    34861 ctggttaaaa aagagcaggt ggttctcatg ctcaatcgcc gtggttattc tagctttgtc
    34921 atgtgtcggg agtgtgggac ggtggatact tgtcccaact gcgatatttc tctgaccttg
    34981 catatggata ccaagactat gaactgccat tattgtggtt tctcgaagga tattcctcag
    35041 gtctgtccta actgtaagag ccacagtatt cgttactatg ggacgggaac tcagaaggct
    35101 tatgatgagc tagcagaact ctttccccaa gctcgcattt tgaggatgga tgtggatacg
    35161 actcgaaaga aaggcagtca ccaagccttg cttgaccagt ttgggcgagg ggaagcggat
    35221 attttacttg gtactcagat gattgcaaag ggtttggatt ttcccaatgt taccctagtc
    35281 ggagttctaa atgcggatac ggccttgaat ctgcctgatt tccgttcttc tgagagaacc
    35341 ttccagctct tgactcaggt ggcaggtcga gcaggtcggg ctgaaaaagc aggtcaagtc
    35401 ttgatacagt cctacaattc gcagcactat gctattcgat ttgccaagga tcaagattac
    35461 gaaggctttt atgcctatga aatgggaatc agacgacaac tcggctatcc accttactat
    35521 ttcacgattg gtattaccct ttctcacaag aaagaagaag aggttttcaa acgtgcctat
    35581 gaagtcatga acattttgcg gtcaggcttg tcagagacca gtcccattct ggggccaacg
    35641 ccaaaaccta ttgcccgtac ccacaacctc tatcattacc agattttaat taaataccgt
    35701 ttagaggatg agctggggcc gaccctcaac caggttttag ccttgactca agaacgggaa
    35761 aatagtgagc tccgtctcag cattgaccat gagccgcaac aatttttata agaaggagaa
    35821 gatatgacaa aactaatctt tatggggacc cccgactttt cagcaacagt cttaaaagga
    35881 cttttgacag ataaccgtta cgaaattcta gccgttgcga cccagccaga ccgtgctgtt
    35941 ggtcgtaaaa aagttatcca agaaacccca gtcaagcagg ctgccaagga agcaggacta
    36001 tctatctacc aacctgaaaa attatctgga agtccagaga tggaagatct tatgaagcta
    36061 ggagcagatg gaattgtgac tgctgctttt gggcagtttc tcccaagcaa actccttgat
    36121 agcatggact ttgctgtcaa cgttcatgcc tccctccttc ctagacaccg tggtggtgcg
    36181 cctatccatt atgccttgat tcaaggggat gaggaagctg gtgtgaccat catggaaatg
    36241 gttaaggaaa tggatgcagg agatatgatt tctcgtcgca gcattccgat cacagatgag
    36301 gacaatgttg gcaccttgtt tgaaaaattg gcgctagttg gtcgtgattt gcttttggac
    36361 actctgcctg cctatattgc tggtgatatc aaacctgaac cgcaggatac gagtcaggtt
    36421 accttctctc caaatataaa gccagaggaa gaaaaactgg actggaacaa aaccaatcgt
    36481 caactcttta accaaattcg tggaatgaac ccctggcctg ttgcccatac tttccttaag
    36541 ggcgaccgct ttaagattta tgaagcccta ccagtagaag gtcagggaaa tccaggtgag
    36601 attctctcta tctgcaagaa agaattgatt gtcgcaacgg ctgaaggggc tctatccctc
    36661 aaacaagtgc agccagctgg taagcctaag atggacattg cttccttcct caacggagtt
    36721 ggacgtacat tgactgtagg agaacgattt ggtgactaaa gtagaaacgg ctagaagttt
    36781 agctctagca gtgctagagg atgtttttgt gaatcaagca tattcaaata ttgccttaaa
    36841 taaacacctc aaggggagtc agctctctgc agcagacaag ggcttagtga ccgagctagt
    36901 ctatggaacg gtagcccgta aactgactct ggaatggtac ctatcccact ttatcgaaga
    36961 cagagaccag ttagacagct ggctctatgt ccttcttctc atgagtgcct accaactccg
    37021 ctatttggac aagattccag atcatgctgt ggtcaatgaa gcagtggaat tagccaaact
    37081 ccgtaaaaaa ggcagtgaaa aattggtcaa cgctgtcctt cgccgtatct tgcgtgaagg
    37141 ctggccagat attgctagca tcaagcgaaa aaacaagcgt gactccattg cctattctct
    37201 cccagtttgg ctagttgcca aactcaagga agaatacgga gaggagagag caaaagccat
    37261 ctttgaaagc ctgttagtgc gaaacaaagc cagtattcgt gtaacagacc taagtcgaaa
    37321 agaggaaatc caagccttgt tggaggcgaa tgattccctt ttagcaacca ctggtctggt
    37381 taaggagcaa gggcattttg cagggcataa tttgtttgcg gatggagcca ttaccatcca
    37441 agacgagtcc agtcagctgg ttgctccgac gcttgattta caaggtgatg agcgagtgct
    37501 tgatgcctgt gcggctccag gtgggaaaac agcccatata gcctcttatc ttacgacagg
    37561 tcaggttact gctctggact tgtacgacca caagttggat ttaattcaag aaaatgccca
    37621 acgtctggga attgaagatc gggttcaaac tcaaaaatta gatgccagaa aggtgcatga
    37681 gttttttgac caggattcct ttgataagat tttggtggat gctccttgtt caggaatcgg
    37741 tcttttgcgc cgaaaaccag atatcaaata caataaagaa acggcagatt tcgcgtcctt
    37801 gcaggaaatt cagttagaaa tattaggtag tgtttgtcaa atactaggca aaggtggtat
    37861 aataacttat agcacctgca ctatcgtctc agaggagaat tttcaagtcg ttaaggcctt
    37921 tttagagagt catcccgagt tcgagcaggt aaaactagaa catgaatgta aggatatcat
    37981 gaaagacggc tgtatcctca ttacacctga attgtatgga agtgatggat tcttcatcag
    38041 tcaatttcgc aagatatcgg attaggaagg aactgacaca tggaaatttc attattaaca
    38101 gatgttggtc agaaacgaac aaataaccaa gactatgtca accactatgt caatagagct
    38161 ggacgtacca tgattatttt agctgatggg atgggaggtc atcgcgcagg gaatatcgct
    38221 agtgaaatgg cggtcacaga cctgggtgta gcttgggttg atacccagat cgatacagtc
    38281 aatgaagtgc gtgaatggtt cgcccattac ctagaaattg aaaatcaaaa gattcaccag
    38341 cttggtcagg atgaagctta cagaggcatg ggaactactt tggaagtcct tgctattatt
    38401 gataatcagg ctatctatgc tcatattggt gattcgcgta tcggcttgat tcgtggagaa
    38461 gaataccatc agttgacgag cgatcattcc ttggttaatg aattgctcaa ggctggtcaa
    38521 ttgacaccag aagaggcaga agctcatccg caaaaaaata ttatcaccca gtctattggg
    38581 caaaaagatg aaattcagcc tgattttggg acagttatcc ttgagtcagg tgactatctc
    38641 ttgctcaata gtgacggctt gaccaacatg atttcaggca gtgagattcg tgatattgta
    38701 accagtgata ttcctttagc agataaaacg gagacacttg ttcgttttgc taacaatgca
    38761 ggaggtttag acaacattac ggttgccctt gtttctatga acgaggagga tgcagaatga
    38821 tccaaatcgg caagattttt gccggacgct atcggattgt caaacagatt ggtcgaggag
    38881 gtatggcgga tgtctaccta gccaaagact taatcttaga tggggaagaa gtggcagtga
    38941 aggttctgag gaccaactac cagacggacc cgatagctgt agctcgtttt cagcgtgaag
    39001 cgagagctat ggcagatcta gaccatcctc atatcgttcg gataacagat attggtgagg
    39061 aagacggtca acagtatctt gcaatggagt atgttgctgg actagacctc aaacgctata
    39121 tcaaggaaca ttatcctctt tctaatgaag aagcagtccg tatcatggga caaattctct
    39181 tggctatgcg cttggcccat actcgaggaa ttgttcacag ggacttgaaa cctcaaaata
    39241 tccttttgac accagatggg actgccaagg tcacagactt tgggattgct gtagcctttg
    39301 cagagacaag tctgacccag actaactcga tgttgggctc agttcattac ttgtcaccag
    39361 agcaggcgcg tggttcgaag gcgactgtgc agagtgatat ctatgccatg gggattattt
    39421 tctatgagat gttgacaggc catatccctt atgacgggga tagcgcggtg accattgccc
    39481 tccagcattt ccagaaaccc ctgccgtccg ttattgcaga aaatccatct gtacctcagg
    39541 ctttagaaaa tgttattatc aaggcaactg ctaaaaagtt gaccaatcgc taccgctcgg
    39601 tttcagagat gtatgtggac ttgtctagta gcttgtccta caatcgtaga aatgaaagta
    39661 agttaatctt tgatgaaacg agcaaggcag ataccaagac cttgccgaag gtttctcaga
    39721 gtaccttgac atctattcct aaggttcaag cgcagacaga acacaaatca atcaaaaacc
    39781 caagccaggc tgtgacagag gaaacttacc aaccacaagc accgaaaaaa catagattta
    39841 agatgcgtta cctgattttg ttggccagcc ttgtattggt ggcagcttct cttatttgga
    39901 tactatccag aactcctgca accattgcca ttccagatgt ggcaggtcag acagttgcag
    39961 aggccaaggc aacgctcaaa aaagccaatt ttgagattgg tgaggagaag acagaggcta
    40021 gtgaaaaggt ggaagaaggg cggattatcc gtacagatcc tggcgctgga actggtcgaa
    40081 aagaaggaac gaaaatcaat ttggttgtct catcaggcaa gcaatctttc caaattagta
    40141 attatgtcgg tcggaaatcc tctgatgtca ttgcggaatt aaaagagaaa aaagttccag
    40201 ataatttgat taaaattgag gaagaagagt cgaatgagag tgaggctgga acggtcctga
    40261 agcaaagtct accagaaggt acgacctatg acttgagcaa ggcaactcaa attgttttga
    40321 cagtagctaa aaaagctacg acgattcaat tagggaacta tattggacgg aactctacag
    40381 aagtaatctc agaactcaag cagaagaagg ttcctgagaa tttgattaag atagaggaag
    40441 aagagtccag cgaaagcgaa ccaggaacga ttatgaaaca aagtccaggt gccggaacga
    40501 cttatgatgt gagtaaacct actcaaattg tcttgacagt agctaaaaaa gttacaagtg
    40561 ttgccatgcc gagttacatt ggttccagct tggagtttac taagaacaat ttgattcaaa
    40621 ttgttgggat taaggaagct aatatagaag ttgtagaagt gacgacagcg cctgcaggta
    40681 gtgcagaagg catggttgtt gaacaaagtc ctagagcagg tgaaaaggta gacctcaata
    40741 agactagagt caagatttca atctacaaac ctaaaacaac ttcagctact ccttaaaagc
    40801 agatggataa tcaaaatgag taatcaatct ttgtcatgat ttcatggcaa agattttttt
    40861 tgagtccaga tttgtgatag aatagaggga gttgataaaa ggaggaaagc atggaagaat
    40921 caaaagaatt aaatgccgtc attgatgtga ttatgctagc ggggactatt ctccttaaaa
    40981 gtggctcaga aatccatcgt gtagaagata ccatgattcg aatcgcgcat tcgcagggga
    41041 ttgtggattg caatgtcctt gccatgcctg ccgctatctt tttctctatt gaaaatacca
    41101 atatttcgcg catgaagcgc gtgacctcct cttcttataa catcgaaaaa gtctgcgatg
    41161 tgaaccagat ttctcgtcag ctagttgggg ggcagattga cttagaaaca gcctttaagc
    41221 aattgacggc cttgcaagct caaccccttc cctatactaa gttgcaggta actctggctg
    41281 cgacctttag tgctcctttc ttttcagtta tgtttagcgg aaatatctac gacgcacttg
    41341 gggcaggagt ggcgacctta tttggttttg ccttttccct ctatgtggaa aaatttatcc
    41401 gaattccctt tgtgacagcc tttgctggag cctttgtctt tgggataatt gcccagtttt
    41461 gggctcgcta cacaggtttt ccttcaacgg cagatttgat tatagctggt gcggtcatgc
    41521 cgtttgtacc aggtattgcc ttgaccaatg cggtccgtga tattatgacc aaccacataa
    41581 actctggtat gagtaagatg tttgaatccc tgctcattac cctcgcttta ggggcaggaa
    41641 cttctgtcgc cttggtattg atgaactaat atgacactaa caaccttttt attacaagca
    41701 gtagcaagtt ttcttgccat tatcactttt ttaatcgtac tcaatgtgca acggtctatg
    41761 ctcttacctg gagggatttt gggcatgact gtctggctaa tctatctctt gctcaaggaa
    41821 ccgaccaatg tcattgtagc taccttcatt gcagccatta ttggttcttg tgtcagccag
    41881 attttaagta ttctttataa gacacctgct gtggtcttta tcttggccat tttggcaccg
    41941 ctggttccag gttatctctc ctaccgaaca actgcctttt ttgtgacagg ggactataat
    42001 aaagcactgg caagtgcgac cttggttgtc atgttggctt tggtaatctc tattggaatg
    42061 gctagcggaa cagtgattct cagactgtat cattatataa aaacacatcg agtatcgtag
    42121 actttacaga aataaaagaa ttttctgaaa aatgagataa ataaattgac aacgctttct
    42181 atatgtgcga gaataccgca cttatgaaga aattgcggct gattttggta tccacgaaag
    42241 caacttaatc cgtcggagcc aatgggttga agtaactctt gttcaaagtg gtgttacgat
    42301 ttcaaaaact catcttagtg ctgagaatac ggtgattgtg gatgcaacag aggtaaaaat
    42361 aaatcgtcct aaaaaaatca actagccaat tattctggta aaatagaaat gtcattctat
    42421 ggaacctcaa gcgattgtca caagtcaagg gagaattgtt tctttggata tcgctgtgaa
    42481 ctattgtcat gatatgaagt tgttcaaaat gagtcgcaga aatatcggac aagctggaaa
    42541 aatcttggct gacagtggtt atcaaggacc catgaagata tatcctcaag cacaaactcc
    42601 acgtaaatcc agcaaactca agccgctaat agctgaagat aaagcttata accatgcgct
    42661 atccaaggat agaagcaagg ttgagaacat ctttgccaaa gtaaaaacgt ttaaaatgtt
    42721 ttcaacaacc tatcgaaatc atcgtaaacg cttcggatta cgaatgaatt tgattgctgg
    42781 cattatcaat tatgaactag gattctagtt ttgcaggaag tctactattt tccttattgt
    42841 ctgtaagtct actgaccttg ttgtttatcc cagtcatggt ttctagttcg ggctcagagt
    42901 ttcaaagtgg atggcaagag catcaattga ttgctgagaa ggttagtaaa acacttgaca
    42961 agacatttga taaggatgtc agagaaattc cgaccagtca gttttatcaa aaatttgtag
    43021 atgagatggg aaggacttac tcaggaaatt tgatcctcca ggagctgata actgtgaatg
    43081 gagcttataa agctacttat atcggtgaac tctctagcaa ctaatcattc tttaaaatga
    43141 tgaccaataa gaggcttgat ttggtgaatc aagtcttttt tctctctttt actgccattt
    43201 gtgataaaat agtatagaac gattttttac atgaatgata aaacagaggt aaatatgaca
    43261 atcggtattg ataagattgg ttttgcgacc agtcaatatg tcttgaaatt acaagactta
    43321 gcagaagcga ggggaattga ccctgaaaaa ttaagtaaag gactcttact caaggaattg
    43381 agtattgcgc ccctaactga ggatatcgtg accttggcgg ccagtgctag tgactctatt
    43441 ttaactgagc aagaaagaca agaagttgac atggtcattg tggcgaccga gtcaggaatt
    43501 gaccagagta aggctgcggc cgtctttgtg catggcttgc tgggcatcca gccctttgct
    43561 cgtagtttcg agattaaaga agcctgttat ggggcgacag ctgccctcca ttatgccaaa
    43621 ttgcatgtgg aaaattctcc ggagtccaag gtattggtca ttgccagtga tattgccaaa
    43681 tatggtattg aaactccggg agaaccaact caaggtgctg gaagtgtagc tatgttgatt
    43741 acacaaaatc cacgcatgat ggcctttaat aatgacaatg tagctcagac ccgtgacatc
    43801 atggatttct ggcgaccaaa ttactcgaca actccttatg taaatggtgt ttattctacc
    43861 caacaatact tggatagttt gaaaacgact tggcttgaat atcaaaaacg ctaccagctt
    43921 actttggatg attttgctgc tgtttgtttc cacttgcctt atcctaaatt agcgctaaaa
    43981 ggcttgaaaa aaatcatgga taagagcctg cctcaagaga aaaaagacct cttacaaaag
    44041 cattttgacc agtctattct ctacagtcaa aaggtgggga acatctacac aggttcactt
    44101 ttccttggac ttttgtctct cttggaaaat acagatagct tgaaagctgg ggataaaatc
    44161 gccctttata gttacggaag tggagctgtg gctgagttct tcagtggtga attggttgaa
    44221 ggatatgaag cttatttgga taaagaccgt ttgaacaagc tcaaccaacg aactgcccta
    44281 tccgttgcag actatgaaaa ggtctttttt gaggaagtaa acttggatga aacaaactct
    44341 gcccagtttg ctggctatga aaatcaagat tttgccttgg ttgaaattct tgaccaccaa
    44401 cgccgttata gcaaggttga aaaataatga agataagttg gaatggattt tctaaaaaat
    44461 cataccaaga gcgcctcgag ctgttaaaag ctcaggcgct ccttagtcct gagagacaag
    44521 ctagtctgga gaaggatgaa cagatgagtg tgactgtggc agaccagctg agtgagaatg
    44581 tggtgggaac tttttctctg ccttattcgc tggttccgga ggtacttgtc aacgatcagg
    44641 aatacaccgt tccctatgtg acagaagaac cctctgtggt tgcggcggcc agctatgcca
    44701 tcaaaatcat caagcgtaca ggtggtttta ctgcacaagt ccatcagcga cagatgattg
    44761 ggcaggtagc cctttatcaa gttgctaatc ctaaactagc gcaagagaag attgccagca
    44821 agaaagcgga gctcttggag cttgccaatc aagcctatcc ttctatcgtt aaacgtggag
    44881 gtggggcgcg tgatctgcat gtcgagcaga taaaaggcga accagacttt ctcgttgttt
    44941 atattcatgt cgatacccag gaagccatgg gtgccaatat gctcaacacc atgctggaag
    45001 ccttgaaacc agtcttagaa gaactcagtc agggacagag tctcatggga atcctgtcca
    45061 actacgcgac tgattctctg gtgactgcaa gctgtcgcat cgcctttcgc tacttgagcc
    45121 gccaaaagga tcaaggacga gagattgcgg agaaaattgc gttggctagt cagtttgcgc
    45181 aggctgatcc ttaccgagct gctactcata ataaaggaat ttttaatggt attgatgcga
    45241 ttttgattgc cactggtaat gactggcgtg ccatcgaagc tggggcccat gcctttgcca
    45301 gtcgagatgg acgctatcaa ggtcttagct gctggacgct ggaccttgaa agagaagaat
    45361 tggtcggtga gatgaccctg cccatgcctg tagcgactaa gggtggctct atcggcctca
    45421 acccacgtgt agctctcagt catgatctac taggaaatcc ttctgccaga gaattagccc
    45481 agattatcgt gtccatcggt cttgctcaaa attttgcagc cctcaaagcc ttggtaagta
    45541 cgggcatcca gcaaggccac atgaaactac aggccaaatc tctagctctc ctagctgggg
    45601 ctagtgaatc tgaagttgct cccctagtag agcgcctcat ctcagataaa acctttaacc
    45661 tagagacagc ccagcgctat ctcgaaaatt taagatcata aaaactcaga cgaatcggtc
    45721 tgagttttct tgtgtttaaa tactttttcc tggtaatagc gtaactggta agaagtaacc
    45781 agttgtggcg acttccttgc cttcaatctt ttgcaagaga agatcaatag tgagacaagc
    45841 aatctcttcc aaaggttgct tgatagtagc caattgaggg tagtaatttt cgataaagta
    45901 ggtcccatca tagccgatga ccttgagctc ttttgggaca gaaatgccca attcttgagc
    45961 gattttaatg accagaatag ctgtcaaatc atccgaagca aaaatggcat ctggtttttc
    46021 ccgggtcaag atattcttga tttccatttc ttttctgacg ggagaaaagt cactggaaac
    46081 attgataata ggagcttttg ggagtacgga tgcaaaacca gcgtggcgca gtccggttgg
    46141 cgaattagaa ttgtcattcc ctgtaatcat gatgatagac tgggcacctg tcttgaccaa
    46201 ggtttgggca gcaagaaccc caccagcata gttgtcagag gagacgacag ggatgtctgg
    46261 cgataggttt cggtcaaagg aaataatcgg cgctgtcaca cgattgtagt cttcgattcc
    46321 taggttgtga ctaccagaaa tgatgccgtc cacctgattg gcttccaaca tttcgatgta
    46381 ttcgcgttcc ttctcagaat catgttcact gttgcagatg atggtcttgt aaccattttt
    46441 gaagagttgg tgttccaatt tatcaatcaa ttctgcatag aaaacattgg aaatattggg
    46501 gaaaatcaag ccgattaact tagctgattt tccttgcaga ctacgagcca ggttgttggg
    46561 tttatagccc aattctctca tggcttcatt gactttttgg atggttttct cagatagata
    46621 ccctttttta ttgataaccc gagaaacggt agtaggactg acgcctgcaa gtttggcgac
    46681 atcagttagt tttgcgacca taatctaatt catagtaagt tccagttggg tttccagatt
    46741 tgatcaggat accattttgg tccgcatgtg ggaagacacg accagaaaat actttttctc
    46801 ctttattgat gaaaatttca aagacagagt tatcgatgaa gattgtagca gtagtagcct
    46861 gattctcgat agggcaagaa cgagttgtcc caaattcttg ggcatactgc tctccagcct
    46921 ggctacgatc cactgtcact tgaccgttta caaggtcaaa gttgattgaa agtcccttac
    46981 cttctttatc agcaagtaag acaatctcgc tctggctatt agcttccaag ttgagttcaa
    47041 gttcgtaagt gttcttggtt tgggaacggt ttgagaaggc ttcttcagaa gcacgaaggt
    47101 ccttaatagc agcgactgga tactggtaga gcttgtcgtc tttgatagtg agttccttga
    47161 ccaaagagaa ggttccttgg tggtcaaaac ggtcagatgg gtaagaaaca tctggcaaac
    47221 caagccagct aactgctaga gcacgcccat caggagcgtt gaaggcttga gttgcatagg
    47281 cttcgaaacc gtaatccatg ttttgaagtt gagacacatc taccattttg gcatttttag
    47341 ggtcaaagga agccccaatc ttatacatat ttggaaagat attatcgtag tctagaactt
    47401 tcttatccaa tccttgtgga cagtagagaa ggacaggatg ttcctctaca aagaccagat
    47461 taggacattc catcatgtag gcagtacggt cgttagcaaa gtcaaggtcg ccaactgctt
    47521 gccagtttgt gtagtcgtta ttgacagcct tgtagagacg gacgaagcct tttttctcca
    47581 agtcctgtcc accgacgaca gcatagtatt gacctttaaa gttaaaaatt tgtggatcgc
    47641 ggaagtggtc agtagagtct gctggctggt caatcaagat cttgtcaatc tttgtaatct
    47701 taccctcctt gtccatcaaa gcaccgatct ggtatgggtg acggatccag ttttcatcgc
    47761 gaacatttcc tgtataaaat aggaataagt tatcgccaaa ttgcatggca gaaccagagt
    47821 aggcaccgtg gctatctaat ggagtatctg gtaaaacttt gattccagtt tctttaaagt
    47881 gaaccaaatc atcactttct agctgtgccc aagatttcaa accgtgggct gcaccaaaag
    47941 gaaaattctg gtaaaagagg atccacttgc catcaaagta agaaaagcca tttgggtcgt
    48001 tgagaagtcc tgtttttggc tcaacatggt aatgagtatg ccatggagat tgtgccatat
    48061 tttcctttat atgttgaatt tcttcttgcg tccaatcttg ataaagtctg taacgacgct
    48121 cagttgtcca ttccattcat tcctccaaaa atcttatcac ttttaatagt aactgttttc
    48181 gataaaaaat gcaagccttt tatattaaaa aagagaaaaa actgtcaaac gattaccata
    48241 aaataaatac aggaaatcaa tcaaattttt acgaaaatgt gaaagataat gaaataaaag
    48301 actttttaac caaggtttca aacttatttt tcaagaaatt cctgatgaaa cagtcaaaaa
    48361 caatcaatct aaagcttgcc cactaagtag atagaatatt ttttctgcaa aacgcttgac
    48421 atatttttaa aacatgataa aataatagtc gtaagggcga ataaaatcgc tttcaaacaa
    48481 gaacaaaatg ttatataagg agatttttgc aaatgaacaa tcaggaaatt gcaaaaaaag
    48541 tcatcgatgc cttgggcgga cgtgaaaatg tcaatagtgt tgcccactgt gcgactcgtc
    48601 tacgtgtcat ggtcaaagat gaagagaaaa tcaataaaga agtgattgag aacttggaaa
    48661 aagttcaagg tgctttcttt aactcagggc aataccaaat tatctttggt acaggtacag
    48721 ttaacaaaat gtacgatgaa gttgttgtac ttggcttacc aacatcatct aaggatgaca
    48781 tgaaagcaga agttgctaaa caagggaact ggttccaacg tgctatccgt actttcggtg
    48841 atgttttcgt tccaatcatc ccagttatcg tagcgacagg tctcttcatg ggtgtgcgtg
    48901 gtcttttcaa cgctcttgaa atgccacttc caggtgactt tgcaacttac acacaaatct
    48961 tgacagatac agccttcatc atcttgccag gtttggttgt gtggtcaacc ttccgtgtat
    49021 ttggtggaaa tcctgccgtt ggtatcgttc ttggtatgat gcttgtctct ggctcacttc
    49081 caaacgcttg ggcagttgct caaggtggtg aagtaacagc gatgaacttc tttggtttca
    49141 tccctgttgt tggtttgcaa ggttccgttc ttccagcctt catcatcggg gttgtcggag
    49201 ctaaatttga aaaagctgtc cgcaaggttg ttccagatgt cattgacctc ttggtaacac
    49261 cattcgtgac acttttggtc atgtctatcc ttggactctt tgtcattgga ccagttttcc
    49321 acgttgttga aaactacatc cttattgcta caaaagcgat tcttagcatt ccatttggtc
    49381 ttggtggttt cttgattggt ggggttcacc aattgatcgt cgtgtcaggt gtgcaccaca
    49441 tcttcaactt gcttgaagtg caattacttg ctgctgacca tgctaaccca ttcaacgcta
    49501 tcatcacagc tgctatgaca gctcaaggtg ctgctactgt tgcggttggt gttaaaacaa
    49561 aaaatccaaa actgaaaaca cttgctttcc cggctgctct ttctgccttc ctaggtatta
    49621 cagagcctgc tatcttcggg gtgaacttgc gcttccgtaa accattcttc ctttcattga
    49681 ttgctggtgc aatcggtggt ggattggctt ctatccttgg acttgctggt actggtaatg
    49741 gtatcaccat catccctggt acaatgcttt atgttggtaa cggacaactt ccacaatacc
    49801 ttcttatggt agctgtatca tttgcccttg gttttgctct tacttacatg tttggttacg
    49861 aagatgaagt agacgcaact gcagctgcaa aacaagctga agtggctgaa gaaaaagaag
    49921 aagttgcgcc agcagctctt caaaatgaaa cacttgtaac tcctatcgtt ggtgatgttg
    49981 tcgctcttgc tgatgtcaat gacccagtct tctcaagtgg agctatggga caaggtatcg
    50041 ctgtgaaacc aagccaaggc gtggtctatg caccagctga tgctgaagtt tcaattgcct
    50101 ttccaacagg gcacgctttt ggtttgaaaa caagaaatgg tgctgaagtt ttgattcacg
    50161 ttggtattga cactgtatct atgaacggtg acggttttga agcaaaagtt gctcaaggta
    50221 ataaggtgaa agctggcgat gttcttggaa catttgactc aaacaaaatc gctgcagctg
    50281 gacttgatga tacaacaatg gttatcgtta caaatacagc tgactacgct tcagtagctc
    50341 cagtcgcaac aggttcagtt gcgaaggggg atgctgtgat cgaagtgaaa atctaatcaa
    50401 tcctctctaa taaaaacgaa caaatgacat gtttgttcgt ttttattaga tagtaagatt
    50461 tacagaggaa aatctaaaaa atagagacat ttagactttc gaagtatgct ataataaaga
    50521 aaataaaaac aagaggttta tcatgacaaa attatatgga agcttggaag cgggcggtac
    50581 aaagtttgtc tgtgctgtcg gtgatgaaaa ctttaacgtt gtagaaaaaa cacaatttcc
    50641 aacaacaact ccaatcgaaa caatcgataa aaccattgag ttcttctcaa aattcgataa
    50701 ccttgctggt cttgcagttg gttcatttgg gccgattgat attgacaaaa actcaaaaac
    50761 ttatggcttt atcacgacga ctccaaaacc aaactgggca aatgtggact tgcttggtgc
    50821 ccttcgtcgc gccctaaacg tgccaatgta cttcacaaca gacgtaaaca gctctgctta
    50881 cggtgaagtg gttgcccgta acaatgctgg tggtcgtatc gaaaacttgg tttactacac
    50941 aatcggtaca ggtatcggtg cgggcgtcat ccaacgtggt gagtttatcg gtggtgtggg
    51001 tcaccctgaa atgggtcatt attatgttgc tagacaccca atggatattg aaaaagagtt
    51061 taagggtgtt tgtcctttcc ataagggatg tctagaaggt tatgcagctg gtccaagttt
    51121 ggaagctcgt acaggtgtac gtggggaaac tattgaactc aacaaccctg tttgggatgt
    51181 tcaagcctac tatatcgctc aagctgcggt taatgcgaca gtgactttcc gcccagacgt
    51241 gattgtcttt ggtggagggg tcatggctca acaacatatg ctggaccgtg tccgtgagaa
    51301 atttacatct cttcttaatg gttacctacc agtaccagat gtgcgtgact atatcgtgac
    51361 gccagcagtc gcaggaaatg gttctgccac acttgggaac tttgttcttg caaaagaagt
    51421 ttcaaaataa agcacaaaat ccgcttggga aaccaaacgg attttgtggt tgaaaacaat
    51481 atttttgagc ctttccttaa gtgattacgg acggtagcga ctttcttcga aattccatac
    51541 ctaaactttg agacactagg ctcaaagttt ccgaatacct gaaaccatta gatttcaggt
    51601 gtttctatca cggcggaaag gctaggtaag tcctcgcctc actatcgaaa aatatgcatg
    51661 atttccatac caaagtatat ttagtatcaa gctatagtta atctacgctt taaaaatcca
    51721 cttgggaaac caaacggatt ttttacgtgt caaagcattt gccagaaaaa gtcaataata
    51781 taggtcaaac cgtaggtata aaccacgagg gtaaagggtt tggtcagaat gatgatggtc
    51841 atgtagcgct tgaaactcat cttggtcagg gcagccagca tacagagaaa gtcagctggg
    51901 ctaatgggcc aaatcatcat aaaaataaag aagcggtcaa aacgattgcc cttatctagc
    51961 cagccgatgt acttgtcgta ggtgcgcttg ctgacgacag actggacaaa ggcagctccg
    52021 tataggcgca ctagataaaa gataatggca cagccaatca cgatgccgat atagttgtag
    52081 atagtcccga tgatgtgccc gtagataaag accccagcca ccgaggtcaa ggcccctgga
    52141 atgataggga cgacagtctg taaaatctgt aaaaagataa agagaggtgg accccagatg
    52201 cctgcctgct ggataaaggc agagagggtt tccttggatt gtaaaatccc agcctgataa
    52261 gcccaaatac agaaaatcaa actcccaacc ccaccgacaa tcgatgagat attgataact
    52321 cgctgcagaa ggggagaaac agctttcatt tgtttatcct gtgacatgta aactcctcat
    52381 agatagtata tttctactat accatatttt ggaggagaaa gggggaatga gtcttttttg
    52441 ataaggaata gaaagtaaat agcccttcga gacttagaat aggtctgtca actaaaggtt
    52501 atatgcattc taggtttcga ataatcgcaa ttccatgaat ttaaaagcat tctaagtttc
    52561 gaatagctta tctgagataa ttttgaggca ttcgcgcttt cgaatagtca ttttccctaa
    52621 agtttagatg tattctgaag ctagaaagac taaaatctaa aagtagtaag agtctctctc
    52681 tgatggacgt aatttatggc tagtatgaat cctttgaatt gttagttaat tttaatatta
    52741 atcagttctg aaataattac taaatatagt tagatggtga attaattatt gctaaatttc
    52801 aagttcaagt tagtcatcca gaagtcttct ctgggggact tgtagttcaa gcatttttta
    52861 ggatagttgt taatccactt ttcgatgaat gcgacttcct ttgtagtcgt tttcttagtt
    52921 ccctgaggta accatctatg aatgaacttg ttgtgatttt tattagatcc tatagtggtg
    52981 tgcataatag atataataga ttgaaactag aatagtacgc atctacttct aaaacattgt
    53041 tagaaatcga tttgactgtc ccgatctatt tgtcctgttt ttatttcatt ttgctatata
    53101 atccttagaa aacatatggg acaagcggtt gaattccgtt ccgtccatta gtagataaag
    53161 gattactcct tcttgaaatg acagatgaac ttgtttctta taaaaatgtt gtctgaaact
    53221 attcgctttt ttccagaagt tttagaggaa gaaaacttta tgaggaaaaa ggagttactc
    53281 aaataacaat tcaggattaa aaatagacag ttgaggagcg gaaggtataa attaagtttg
    53341 ctgctgtata atggatttat cactaatact cttcgaaaat ctcttcaaac cacgtcagct
    53401 tcgccttgcc gtagatatag ttactgactt cgtcagttct atccacaacc tcaaaacagt
    53461 gttttgagct gacttcgtca gttttatctg caacctcaaa gctgtacttt gagcagtcta
    53521 cggctagctt cctagtttgc tctttgattt tcattgagta taaaagtgct ccaggccata
    53581 ttttacacag caatagtctt ctaaaaatga acaaaaaaca ttgtaaaggc tatcaaaaag
    53641 gagtataatg agtgcaagaa atttcggagg tgaaagatgc tagaagtaag aagtctagag
    53701 aaaagttttg gatccaagca agttttgttt ggtattgact ttcaagcgcg accaggtcgt
    53761 attttgggac tagtcggaaa aaatggtgct ggaaaaacaa cgattttcca tagtatttta
    53821 aaattcctag aatatcaggg agaaatcggt ctggatggtc aggatattcg tcaggagacc
    53881 tatgctcgga ttggttatct gcctgaagaa cgcagtctca tgcctaaatt gacagttctt
    53941 gaacaagttc gctacttggc gactctaaaa ggcatggatg ccaaagaagt caaagaaaaa
    54001 ctccctcaat ggatgaagag gttggaagtg aaaggaaagc tggcagataa aatcaagagt
    54061 ttgtcaaaag gaaatcagca gaagattcag ctcattatta ctctgattca tgaaccagac
    54121 ctgattatct tggatgagcc ttttagtgga ttggacccag ttaatacaga attgctcaaa
    54181 caagtcattt ttcaggaaaa agagcgcgga gcaaccatta tcttttctga ccatgtcatg
    54241 accaatgttg aggaactttg tgacgatatt ctgatgatcc gagatggccg tgtggtcttg
    54301 cacggaccag ttcaggatgt ccgcaatcaa tacgggaaaa cacgtctctt tgtttcaagt
    54361 gaacgaagca aggaagaatt ggaaaatctt cctcatgtca aacaggtgag cttgaccaaa
    54421 caaggcagtt ggaaattgat tttggaggat gagagcgctg gaagggaact cttcccaatc
    54481 ttgactcaag gtcaatatat cgcaacattt gaccaacaag cgccaactat cgatgaaatc
    54541 tttaaactag aatcaggagt ggaagtatga gaaatatgtg ggttgtaatc aaggaaacct
    54601 atcttcgaca tgtcgagtca tggagtttct tctttatggt gatttcgccg ttcctctttt
    54661 taggaatctc tgtaggaatt gggcatctcc aaggttcttc tatggctaaa aataataaag
    54721 tggcagtagt gacaacagtg ccatctgtag cagaaggact gaagaatgta aatggtgtta
    54781 acttcgacta taaagatgaa gcaagtgcca aagaagcaat taaagaagaa aaattaaaag
    54841 gttatttgac cattgatcaa gaagatagtg ttctaaaggc agtttatcat ggcgaaacat
    54901 cgcttgaaaa tggaattaaa tttgaggtta caggtacact caatgaactg caaaatcagc
    54961 ttaatcgttc aactgcttcc ttgtctcaag agcaggaaaa acgcttagcg cagacaattc
    55021 aattcacaga aaagattgat gaagccaagg aaaataaaaa gtttattcaa acaattgcag
    55081 caggtgcctt aggattcttt ctttatatga ttctgattac ctatgcgggt gtaacagctc
    55141 aggaagttgc cagtgaaaaa ggcaccaaaa ttatggaagt cgttttttct agcataaggg
    55201 caagtcacta tttctatgcg cggatgatgg ctctgtttct agtgatttta acgcatattg
    55261 ggatctatgt tgtaggtggt ctggctgccg ttttgctctt taaagatttg ccattcttgg
    55321 ctcagtctgg tattttggtt cacttgggag atgctatctc actgaatacc ctgctcttta
    55381 ttttgatcag tcttttcatg tacgtagtct tggcagcctt cctaggatct atggtttctc
    55441 gtcctgagga ctcagggaaa gccttgtcgc ctttgatgat tttgattatg ggtggttttt
    55501 ttggagtgac agctctaggt gcagctggtg acaatctcct cttgaagatt ggttcttata
    55561 ttccctttat ttcgaccttc tttatgccgt ttcgaacgat taatggctat gcggagggag
    55621 cggaagcatg gatttcactt gctattacag tgatttttgc ggtggtagca acaggattta
    55681 tcggacgcat gtatgctagt ctcgttcttc aaacggatga tttagggatt tggaaaacct
    55741 ttaaacgtgc cttatcttat aaatagaaga gccttgcgaa agcgaggttt ttaaaggtga
    55801 aaagggtgga atcacgaacc taattatgta cacattatgt aaacaggttg cttgaaaact
    55861 caattgtata gtgaactgaa tctggaatag gataatatta cttctaaaac attcttataa
    55921 attgatttga attccaaaat cccatcgttc cgttcttatt tcattttgcg atatagcgaa
    55981 aggaaataag tcagctcaca aaaaacgcat aatatcaagg atttttaaca cttgatacta
    56041 tgcgttttct aattttaaag actttttgcc tggtctcttt tgttctatga gtatgttaca
    56101 tatctaggta aaaattattt aaagcaaatt aaccttattt ctcaagatgt agtaggttcc
    56161 gagataaaag atagctatga atatgagttc tttaacaatg gctatacctg ctcccatata
    56221 gaaatggtca ttgagtccta ccagtgaatt gacatagaaa ttggaactaa gattgaagaa
    56281 aagttcaata aatccaatga cgatttggat accaatgtag gctgcaacag cgagtgctgt
    56341 acggtattca ttgaaaagct gtccaatgga aatagccagg tagatgcaga ggattcctga
    56401 aatggtattt agtaggaagg atatacctgt aagaaagatc tgagggagat gtgtttctac
    56461 aaatgtaatc acataagaaa gaggaatcca ttctggagct gttaaagcca gaataataac
    56521 agcacttaga gccaatacag cggtgctaat caatgaccag ataaaggcac cgattagttt
    56581 ggctgtgatg atatggtgtt cagaaactgg caaggtcaaa gtcagatagc cttgtcggtc
    56641 gtagacacta cctttgaagc gtttaataat caagaaaatg gttgaaatcg caagtgtaag
    56701 catcaagcca ccaaagactg tagctagaaa aagtagcata gtagcctgac tttcttggta
    56761 aggtagattt ttaaagcctt gtgtctgtat tccgatgagg gcagaaagga ctagcacggc
    56821 tgcgtagagg gctaaatacc acttgttaac atttttaaat tcgtagtgaa ctaaattcca
    56881 aaacataaat aatctccttt gcttaggcct taaattcctg acggaagagt tggtcaatgg
    56941 attcacctga ctcgtagcga atatcatcta catttccttg acggacgact tttccgtctt
    57001 ttaggaagac aatttcatcc aagattggct cgatatcaga aatcaagtgg gtagaaatca
    57061 aaacggtaga agttggtgag tagttgttga taatggtatt gaggatataa gcacgggctg
    57121 ctggatccac cccaccaatg ggttcatcca aaacatagag acgagcatca cggctcataa
    57181 ccaaaatcag ttgaaccttt tctttgtttc cttttgatag tttcttgaga cgactatttt
    57241 catcaatgcc caggtctgca agtagatgat gagcgcgttc aagattgaaa tctttataga
    57301 aggtcttgaa gtaggttagg gcttctttga ccttcatttg ctcattgaga taggtcgtat
    57361 caggcaaata agctacaacg gccttggttg ctgggcttgg gtccatgtcg ttgatgagga
    57421 cacgtccttg atctggttgt aagaggccat taattagttt aatcagggtt gtttttcctg
    57481 agccgtttgg cccaagaagg ccgacaattt ttccagctgg aatgtcaaga gaaacatttt
    57541 caagggctgg tgttgctcca tatgatttgg atacattttc aaatgctagt aatgacatag
    57601 gcttaaactc ctttaatata atcactgact acgcctggta gttcttcttt ttcatagcca
    57661 aaatgggtca tggaggaaac gaagtgttcc aattcttctt ctgataattg tttgcgtgac
    57721 tgggcgatta gctccttatc cttagtcaca aatcgtccag ttgttcgctt gctgtagaca
    57781 aatccttctc gttcaaggtc tgataaggct ctttggatgg tattgggatt gacaccagcc
    57841 tcgctagcta gctccctcac ggttggaagt tgttgattgg gttccagtgt atgggaaaca
    57901 atctgaagct tgattttctc cataatctgt aaatagatgg gttttttgtt gtcaaatgtc
    57961 caggacatct tagtctcctt tcttttaccc ttttgtctta attaaataat acaacaaaac
    58021 aaaaaacttg tcaagcaaaa agaagaattg ttttgggaat cgcttaaaaa atggtataat
    58081 gaaaagaaaa cgataaggag ggaaaggatg cgttgtccaa aatgtggggc taccaagtca
    58141 agtgttatcg atagtcgcca agcagaagaa gggaacacca ttcgtagaag acgtgagtgc
    58201 gacgaatgcc aacaccgttt tacaacctac gaacgagtag aagaaagaac cttagtggtt
    58261 gttaaaaaag atggcacacg ggaacaattc tccagagata aaatctttaa tgggattatc
    58321 cgctcagccc agaaacgtcc tgtgtcaagt gatgaaatca acatggtagt caatcgtatc
    58381 gaacagaaac tccgtggtcg aaatgaaaat gaaattcaaa gtgaggacat tggttcactc
    58441 gtcatggagg agttggctga attggacgag attacctatg tacgttttgc tagtgtctat
    58501 cgtagtttta aggatgtcag tgagttagag agcttgctcc aacaaatcac ccagtcctct
    58561 aaaaagaaaa aggaaagata aatgaagcca attgaccgtt tttcttatct aaagaataat
    58621 cgggtgtcgc aagatacctc atctctggta cagtgctacc tcccgattat cggtcaggag
    58681 gcactgagcc tttatcttta tacaatcagt ttttgggata atggtagaaa ggaatatctt
    58741 ttttcaagca ttctcaacca tcttaacttt gggatggata gactgataaa atcattgaaa
    58801 atcttatctg cttttaatct cttgaccctc tatcaaaagg gggatgttta tcagctagtc
    58861 ctccatgctc ctctatctag tcaagacttc ttggagcatc ctgtttatcg cagactctta
    58921 gagaaaaaga ttggcgatgt agctgtggag gatttgaaag ttgaaagtgc tgatggtgaa
    58981 gaaatacctg tctcactcaa tcaagtcttt ccagaattgg cagaactagg cagtcaagaa
    59041 gaccttggtc tcaagaagaa agtggccaac gattttgact tggaccattt tcgccagctg
    59101 atggctcgag atggacttcg ttttgcggat gagcagtccg atgtcttaaa cctctttgct
    59161 attgccgagg agaagaaatg gacttggttt gaaacctatc aattggccaa gtcaacagct
    59221 gtttctcagg ttatttcaac caaacgcatg cgggaaaaaa ttgctcaaaa accagtttcc
    59281 tctgacttta gtcttaagga aacaaccatt atcaaagaag ccaaaagtaa aactgccctg
    59341 cagttcttgg cagaaatcaa gcaaacacgc aagggaacca ttacccaaac agaaagagaa
    59401 ctcttgcaac agatggctgg cttgggcttg ctggacgaag tcatcaatat cattctttta
    59461 ttgaccttta ataaggtcga ttcggcaaat atcaatgaga aatatgccat gaaggtagcc
    59521 aatgactatg cctatcaaaa gattcattcg gcagaaaagg cagtcttgcg catccgtgag
    59581 cgaggacaga aagcaaaaat acaaaaacag aatcagactg ccccagtaaa aaccaatgtt
    59641 cctaaatgga gtaatcctga atataagaat gaaaccagcg aggaaactcg tctggaacta
    59701 gaacgtaaga agcaagaact attagctcga ttagaaaaag gaggagatta gatggaaagt
    59761 gtcggagacg tactcaaacg tcaacccagc cgtttttatt atcaagattt ggtccagaaa
    59821 atcatgaagg accctgatgt tgcggctttt atccagcaag aatccttgac tccagaggaa
    59881 ttaaatcgca gtatctccaa gtttaatcag tacatcaccg agcgtgacaa gtttctccgt
    59941 ggggatacgg attatattgc caaaggctac aagccgattt tggttaagaa tcatggctat
    60001 gcagatgttt catatgaaga aactcctgag ctaatcgcgg ctgaaaaaga agcggctatt
    60061 aagaaccgtc tcaagttaat caatctacca gccagtctca agaaagctag tttggctcaa
    60121 gttgacttgg atgatttggg gcgcttacca gtttttgaaa agctactagc cttcgtggag
    60181 caatatccag ctattcgaaa aggtctttac ttatatggag actttggtgt gggtaaaagt
    60241 ttcatggtgg cggccttagc tcatgattta tcagaaaaac gtggtgtttc atcaactctc
    60301 ctccactatc ctagctttgt cattgatgtc aaaaatgcta tcagtgatgg caatgttaag
    60361 accttggtgg atgagattaa gctgtctgag gtcctgattt tagatgatat tggcgccgag
    60421 caatcaaccg tttgggtgcg tgacgaaatc ctgcaggtca ttctccaata tcggatgcag
    60481 gaaaatttac cgaccttttt cacatccaac ttcaactttg aagatttgga gaagcatttt
    60541 gcgaaagtga aacatggaaa tgacgaaacc tgggaagcca gacgggtcat ggaacgcatc
    60601 cgttatttgg ctgaggagac tcgtttagaa ggagtaaacc gtcgatgaca gaaaccatta
    60661 aattgatgaa ggctcatact tcagtgcgca ggtttaaaga gcaagaaatt ccccaagtag
    60721 acttaaatga gattttgaca gcagcccaga tggcatcatc ttggaagaat ttccaatcct
    60781 actctgtgat tgtggtacga agtcaagaga agaaagatgc cttgtatgaa ttggtacctc
    60841 aagaagccat tcgccagtct gctgttttcc ttctctttgt cggagatttg aaccgagcag
    60901 aaaagggagc ccgacttcat accgacacct tccaacccca aggtgtggaa ggtctcttga
    60961 ttagttcggt ggatgcggct ttggctggac aaaatgccct gctggcagct gaaagtttgg
    61021 gttatggtgg tgtgattatt ggcttggtac ggtacaagtc agaagaagtg gcagaactct
    61081 ttaacctgcc tgactacacc tatcctgtct ttgggatggc actaggtgtt ccaaatcaaa
    61141 ctcatgatgt gaaaccgaga ctgccactag ataatgtagt ctttgaggaa gaataccaag
    61201 aacagtcaac tgaggcaatc caagcttatg accgtgtgca ggctgactat gctggagcgc
    61261 gtgcgaccac aagctggagt cagcgtctag cagaacagtt tggccaagca gaaccaagct
    61321 caactagaaa aaatcttgaa cagaagaaat tattgtagaa agtgagaaat tatggcccta
    61381 ccaactattg ccattgtagg acgtcccaat gttgggaaat caaccctatt taatcggatc
    61441 gctggtgagc gaatctccat tgtagaagat gtcgaaggag tgacacgtga ccgtatctat
    61501 gcaacgggtg agtggctcaa tcgctctttt agtatgattg atacaggagg aattgatgat
    61561 gtagatgctc ctttcatgga acaaatcaag caccaggcag aaattgccat ggaagaagca
    61621 gatgttatcg tttttgtcgt gtctggtaag gaaggaatta ctgatgcaga cgaatacgta
    61681 gctcgtaagc tttataagac ccacaaacca gttatcctcg cagtcaacaa ggtggacaac
    61741 cctgaaatga gaaatgatat ctatgatttc tatgctctcg gtttgggtga accattgcct
    61801 atctcatctg tccatggaat cggtacaggg gatgtgctag atgcgatcgt agaaaatctt
    61861 ccaaatgaat atgaggaaga aaatccagat gtcattaagt ttagcttgat tggtcgtcct
    61921 aacgttggaa aatcaagctt gatcaatgct atcttgggag aagaccgtgt tattgctagt
    61981 cctgttgctg gaacaactcg tgatgccatt gatacccact ttacagatac agatggtcaa
    62041 gagtttacca tgattgatac ggctggtatg cgtaagtctg gtaaggttta tgaaaatact
    62101 gagaaatact ctgttatgcg tgccatgcgt gctattgacc gttcagatgt ggtcttgatg
    62161 gtcatcaatg cggaagaagg cattcgtgag tacgacaagc gtatcgcagg atttgcccat
    62221 gaagctggta aagggatgat tatcgtggtc aacaagtggg atacgcttga aaaagataac
    62281 cacactatga aaaactggga agaagatatc cgtgagcagt tccaatacct gccttacgca
    62341 ccgattatct ttgtatcagc tttaaccaag caacgtctcc acaaacttcc tgagatgatt
    62401 aagcaaatca gcgaaagtca aaatacacgt attccatcag ctgtcttgaa cgatgtcatc
    62461 atggatgcca ttgccatcaa cccaacaccg acagacaaag gaaaacgtct caagattttc
    62521 tatgcgaccc aagtggcaac caaaccacca acctttgtca tctttgtcaa tgaagaagaa
    62581 ctcatgcact tttcttacct gcgtttcttg gaaaatcaaa tccgcaaggc ctttgttttt
    62641 gagggaacac cgattcatct catcgcaaga aaacgcaaat aaaaaagtag aatctggaat
    62701 gacagttccg gatttttttg atataataaa ataatagaaa acgctatcaa aagaaggggg
    62761 gatgtcggtg atggaacatt tgtttaaatt cttactttta gcaccgtact tttattttga
    62821 taactggatt gaaaaggcca acagaaatag taagtttttt ccaatttttt attattttta
    62881 ctggttttac atccccttct attctctttt tagccttgct tggacagttg tttcagttct
    62941 gtttttcaat accgtcttga gaaatgtgac agatatcaag ttatggggca tttggtttct
    63001 ttttattctg ctagctattg gtatgaattg gttaacttat tcctgtttca aagaaatgtt
    63061 tcgcttgaga caggaactag ggaagtctaa gggtggaagg cattgattta tatcggtttc
    63121 tatttgtagg aggtggctta tggtacctct aaaatcattt attacacgct attccaaggt
    63181 ttatattggt aataacattt aatagaacga aactaaagaa aataatttaa aagaggaggt
    63241 ttgtgatgga acacttgttt aaaatgataa tcttattacc ctgtttttac tttttcagct
    63301 ggattgacaa ggataataga gaaagtaaat ttttcccaat tttttactat ttttactgga
    63361 tttacatcac cctctatgct ctttttagcc ttgcttggac agttttttca gttctgtttt
    63421 tcaatatcgt cttgagaaat ttgacagata tcaagttatg gggtatttgg ctattattac
    63481 tacttatcgc ttttgctagt gactggttag actatatctg tttcaaaaaa atgcttgact
    63541 tgagacggga actagggaag tctaaaggcg gaaggcattg atttatactc ttcgaaaatc
    63601 tcttcaaacc acgtcaacgt cgccttggat tatatatgta actgacttcg tcagtcttat
    63661 ctacaacctc aaagcagtgc tttgagcaac ttgcggctag tttcctagtt tgctctttga
    63721 ttttcattga gtattatatt actttctatt tgtaggaggt ggcttatgaa gattcctctc
    63781 ttaactttgg caaggcataa atttgtttat gtcttgctta ctttgctttt tcttgctttg
    63841 gtttatcgtg atgttttgat gacttatttc ttttttgata ttcatgcgcc cgatctagct
    63901 aaattcgatg gacaagcaat taaaaatgac ttattaaaat cagcattaga ttttcgtatt
    63961 ctccagttca atctaggttt ttatcaatca tttattattc caatcatcat tgttttgcta
    64021 ggttttcaat atattgagct gaaaaataaa gttttacgat tgagtattgg aagagaagtg
    64081 agttatcaag ggttaaaaag aaagttgact ttgcaagttg caagtatccc ttgtttgata
    64141 tatttagtga ctgtgctgat aattgcaatt ttaactcatt tctttgggac tttttctcct
    64201 cttggatgga attctctatt ttctgatggg agtagtttac aaaggctcct agatggagag
    64261 ataacaagct atttgttctt tacttgtgtc ctactaatcg gtattttcat caatgcagtc
    64321 tattttttac aaatagttga ttatttagga aatgtgactc gttcggcaat cacctatttg
    64381 atgtttcttt ggcttggttc tatgctgctc tatagtgcct tgccttacta tatggttcct
    64441 atgacgagtt tgatgcaagc tagctatggg gatgtaagtt tgatgaaact ctttactcct
    64501 tatatccttt atattgtctc ttacatggtg cttgaaaaat atgaagataa tgtttaagaa
    64561 ttttaacaat attttgctaa agagaaagat tgttttacta cttcgtatag ttctgatgat
    64621 gattttgata aaccatctat tgtcaacagc ggttcaaaag caggatgctg ttatcttttt
    64681 caagagagaa ttgatttcaa ttttttccta taatgactat tctgaagtga atttagaaat
    64741 ccccaaactc ttgttaaacc tttcgctttt catggtagga tggctctctg tcattttact
    64801 tgaaagtgat ttggcagacc attaccatca cttgattcgc tatcaatcaa gctccttttt
    64861 cgattataca aggaaacgat tggttgtcat ttctaaattt tttactcaag atttgtttgt
    64921 ctggtttctt ggtttacttc ctctaggaat tcatttcaaa acagtcgcac ttttcttttt
    64981 acttgctcag ttaatgatgt tgtacttact actgtcttat ctgatagcac tgattagtgc
    65041 gggcgctggt ttttcctttt ttctctattt tttagcattt gtgggacaag aatggatgat
    65101 ggatcatatt gtaacagtgt atttactact cttaagttta ttagttatgt tgattgttag
    65161 tcgcttggaa gagaaattta agaaaggata aacgatgaga cttgaaatta taaatggaca
    65221 gaaaatttat aggaaaagac ctattttaaa tcagttgaat ttggtgtttc aatcaggaaa
    65281 aatttatgga cttaaaggtg ataatggatc tggcaagacg gttcttttaa agatacttgc
    65341 tggttatatt aagcttgaca aaggaaaagt tcttcaagat ggtaaagttt acggggtaaa
    65401 aaatcattat attcaggatg caggaatttt aattgaaaaa gtcgagtttt tatctcattt
    65461 atccctgaga gaaaatttgg aactgttaag gtatttttca tctaaagtta cggaaaaaag
    65521 aattgcctat tggattcaat actatgattt acaggaattt gaagacattg aataccgtca
    65581 tttatcctta ggaacaaagc aaaaaatggc cttgattcaa gcctttattt cctctccttc
    65641 tatactcttt ctcgatgaac ctatgaatgc tttggatgag aagagtgtga ggttaaccaa
    65701 acaggtcatt ttatcttacc tgaaaaaaga aaatggtctg gttatcctga cgtcgcacat
    65761 atcggaagat atttcagacc tttgtacaga tgtattagtt gtcgaaaatg gacatataca
    65821 aatgtaaagg atatacaatc ctaggagatg gcttatggca catctaaaat catttattac
    65881 acgctattcc aaggtttata ttggtttagt tctgctgatc tggctgtctt tcttctttat
    65941 cccttgggat aaaccacttc tggggataag gattgacatc ttcatcatac agaaaatctt
    66001 gctagctttt ggaattctgt ccattctcat ggccttgctg tccaagaaag tcagtctctt
    66061 tgtttttgga ctgatttgct gtctttctct ttggattaac ttatttatca cacttgccat
    66121 tttgccgatt tttggcaatt aaacagtcat aaaagtcgga gaggttagct tgaaaactaa
    66181 cctctttttc cttttcaaaa tggggattct tccttgaaaa taatcagtaa ttgtgctaaa
    66241 attaaaggaa cattctaaaa tattcggaat ttaaagtaag gaaaaacatg gctaatattt
    66301 taaaaacaat tatcgaaaat gataaaggag aaatccgtcg tctggaaaag atggctgaca
    66361 aggttttcaa atacgaagac caaatgactg ctttgactga cgaccaacta aaagcaaaaa
    66421 cagttgaatt taaggaacgt tatcaaaatg gagaatcact ggattcattg ctttacgaag
    66481 catttgcggt tgtccgtgaa ggtgccaaac gtgtcctagg tctcttccct tataaggttc
    66541 aggtcatggg ggggattgtt cttcaccatg gtgacgtgcc agagatgcgt acaggggaag
    66601 ggaaaacctt gactgcgacc atgccggtat acctcaatgc cctttcaggt aaaggggttc
    66661 acgtagttac ggttaatgaa tacctgtcag aacgtgacgc gactgagatg ggtgaattgt
    66721 actcttggct tggtttgtca gtagggatta acttggctac caaatctcca atggagaaaa
    66781 aagaagccta tgagtgtgat attacttact caactaactc agaaatcgga tttgactacc
    66841 ttcgtgacaa catggtcgtt cgcgctgaaa acatggtaca acgtccgctt aactatgcct
    66901 tggtcgatga ggttgactct atcttgattg acgaggctcg tacacctttg attgtatcag
    66961 gtgccaatgc ggttgaaacc agtcagttgt atcacatggc agaccactat gtaaaatctt
    67021 tgaacaaaga tgactacatc atcgatgtgc agtctaagac tattggtttg tctgattcag
    67081 ggattgacag ggctgaaagc tacttcaagc ttgaaaacct ctatgacatc gaaaacgtgg
    67141 ctttgaccca ctttatcgat aacgcccttc gtgccaacta catcatgctt ctcgatattg
    67201 actatgtggt gagcgaagag caagaaatct tgattgtcga ccaatttaca ggtcgtacca
    67261 tggaaggtcg tcgttattct gatggattgc accaagctat tgaagccaaa gaaggtgtgc
    67321 caatccagga tgaaaccaag acatctgcct caatcacgta ccaaaacctt ttccgtatgt
    67381 acaagaaatt gtctggtatg acgggtacag gtaagactga ggaagaagaa ttccgtgaaa
    67441 tctacaacat tcgtgttatt ccaatcccaa caaaccgtcc tgttcaacgt attgaccact
    67501 cagaccttct ttatgcaagt atagaatcta agtttaaagc ggttgtcgaa gacgttaagg
    67561 ctcgttacca aaagggtcaa cctgtcttgg ttggtacagt agcggttgaa actagtgact
    67621 acatttctaa gaaattggtt gcagctggtg ttcctcacga agtcttgaat gccaaaaacc
    67681 actatagaga agcccaaatc atcatgaatg ctggtcaacg tggtgccgtt accatcgcaa
    67741 ccaacatggc gggtcgtggt accgacatca agcttggtga aggtgttcgt gaacttggag
    67801 gactttgtgt tattggtaca gaacgtcatg aaagtcgtcg tatcgataac cagcttcgtg
    67861 gacgttcagg tcgtcaagga gatccaggtg agtcacaatt ctacctatct cttgaagatg
    67921 atttgatgaa acgttttggt tctgaacgct tgaagggaat ctttgaacgc ttgaacatgt
    67981 ctgaagaggc gattgagtct cgcatgttga cgcgtcaggt tgaagcagct cagaaacgtg
    68041 tcgaaggaaa taactacgat acccgtaaac aagtccttca atacgatgat gtcatgcgtg
    68101 aacaacgtga gattatctat gctcaacgtt acgatgtcat cactgcagat cgtgacttgg
    68161 cacctgaaat tcagtctatg atcaaacgca cgattgaacg tgtcgttgat ggtcatgcgc
    68221 gtgccaaaca agatgaaaaa ctagaggcaa ttttgaactt tgctaagtac aacttgcttc
    68281 ctgaagattc tattacgatg gaagacttgt caggcttgtc tgataaggcc atcaaggaag
    68341 agcttttcca acgtgccttg aaggtttacg atagtcaggt ttcaaaacta cgcgatgaag
    68401 aagcagttaa agaattccaa aaagttttga ttctacgagt agtggataac aagtggacag
    68461 atcatatcga tgcccttgat caattgcgta acgcggttgg acttcgtggc tatgctcaga
    68521 acaaccctgt tgttgagtat caggcagaag gtttccgtat gtttaatgat atgattggtt
    68581 cgattgagtt tgatgtgaca cgcttgatga tgaaagcaca aattcatgaa caagaaagac
    68641 cacaggcaga acgtcatatc agtacaacag cgactcgcaa tatcgctgct caccaagcaa
    68701 gtatgccaga agatttggat ttgagccaga ttggacgcaa tgaactttgc ccatgtggtt
    68761 ctggtaagaa atttaaaaac tgtcacggta aaagacaata aaatgagata gtttagaggc
    68821 ggatatcttg tgaaaagtaa atttttactg ggtatccgtt tgctttataa ggagatgagt
    68881 tatggtattt acagcaaaaa gctctaaaat aaatatagaa gaagttcgtg ccttgtcaaa
    68941 attagaaggt caggctttgg agaggaaatc acagcgagat caagagctag aagccattat
    69001 acgtggagaa gaccagcgaa ttctcttggt aatcgggcca tgctcatctg acaacgaaga
    69061 agctgtcctt gaatacgcta agcgtttggc agtcctacaa gaagaagtgg cagatcgtat
    69121 ctttatggtt atgcgtgttt atactgccaa accccgtacc aacggagatg gctataaggg
    69181 cttgattcac cagcctaacg cgacagaagc gcctagtctt atcaatggaa tcaaagccgt
    69241 tcgccatctt cactatcgtg tcatcacaga aacagggatg acaactgctg atgaaatgct
    69301 ttatcctgaa aaccttccgc ttgtagatga tttgatttct tacatggcag ttggtgcccg
    69361 ttcagttgaa gaccagcaac accgctttgt ggcaagtggg gcaggatttt ctactggttt
    69421 taaaaatcca acctctggaa atctcaatgt catgtttaat gggatttatg ctgctcaaaa
    69481 caaacaaagt ttccttttct taggaaaaga agtagaaaca actgggaacc cgctttcaca
    69541 cgctattctt cgtggtgctc ttaatgagta tggaaaaaat attcccaact actattatga
    69601 caatttaatt gataccattg cccagtatga gaaaatgggc ttggaaaatc cttttatcat
    69661 cattgatacc aatcatgaca attctggtaa gcagtatatt gaacagatcc gaattgtccg
    69721 ccagaccttg attaaccgtg cttggaatga aaaaattaag cagttcgttc gtggttttat
    69781 gattgagtct tatctggaag atggtcgaca aaatgagcca gaagtatttg gtaagtctat
    69841 cacagaccct tgcctgggtt gggataacac agaagctctt gtcagagaaa tttacaaaac
    69901 gttaggagaa taagatggca tttattgaaa aaggtcaaga aatcgatatg gaagtcatca
    69961 aggctgaaac ccaattgtct gcggaagcct tgagactcaa ggaaagccgt gacagggaat
    70021 tggcagatat tatttcaggg gaagatgacc gtattctctt ggtgattggt ccttgctctt
    70081 ctgataatga agaggcggtc ttggaatatg ctcgccgttt atccgccttg caaaagaagg
    70141 tagcggacaa gattttcatg gtcatgcgcg tgtatactgc taagcctcgt accaatggag
    70201 acggctataa aggattagtt caccagccag atacttctaa ggctccaagc ctgattaatg
    70261 gcttgcaggc tgtgcgccag ttgcactacc gcgtgattac agagactggt ttgacaacgg
    70321 cagatgagat gctttatccg tcaaatctga tcttggtgga tgacttggtc agctaccatg
    70381 ccgttggagc tcgttctgtg gaagaccaag agcaccgctt tgtggcttct gggattgatg
    70441 caccagtagg gatgaaaaat ccaacctcag gaaatttggg tgttatgttt aacgccatct
    70501 atgctgctca aaacaagcaa accttccttt atcatgggca ggaagttgag acatcaggta
    70561 atcctttggc ccatgttatc ctccgtggag cagtcaacga gtatggcaat tatatgccga
    70621 attactacta tgaaaaccta ctccaagcca ttgaacgcta tgaaaccatg ggacttgaaa
    70681 atccttttat cctcattgac accaaccatg ataactcagg caagcaatat atggagcaga
    70741 ttcgaattgt tcgccagacc ttgcagaatc gtgattggaa tgagaaaatt aaaaagacgg
    70801 ttcgaggatt tatgattgaa tcttacctag cagatggtcg tcaaaaccaa ccagagatct
    70861 ttggttgctc tattactgac ccttgcctag gttgggaaaa tacagaggcc ttggtagaag
    70921 agatttatgt taccttgaca aaataagtga aaaggatgga gttggggaat ctcaactcct
    70981 tttgatgaga atgatagttg gacacggaat tgacatcgaa gaattggctt cgatagaaag
    71041 tgcagttaca cgacatgaag gatttgctaa gcgtgtactg accgctcagg aaatggagcg
    71101 cttcaccagt ctcaaaggac gcaggcaaat agaatattta gctggtcgct ggtcggctaa
    71161 ggaggccttt tccaaggcta tgggaacggg cattagcaag ctcggttttc aggatttgga
    71221 agtcttgaac aatgaacgtg gggcgcctta ttttagtcag gcaccatttt caggaaagat
    71281 ttggctgtct atcagccaca ccgatcagtt tgtgacagcc agtgtcattt tggaggaaaa
    71341 tcatgaaagc tagtccacat agaccaacca aggctctgat tcatctggga gctattcgac
    71401 aaaatattca gcaaatgggg gctcatatcc ctcaaggaac gctcaagttg gctgtggtta
    71461 aggccaatgc ttatggtcat ggagctgttg ccgttgccaa ggcaattcaa gatgatgttg
    71521 atggcttttg cgtttccaat atcgatgaag ccattgaact cagacaagct ggactcagca
    71581 agccaattct cattttagga gtttctgaaa tcgaagctgt tgctctagct aaagaatatg
    71641 acttcacctt gacagtggct ggactggagt ggattcaagc actcttagat aaggaagtgg
    71701 acctaactgg attgacagtc cacctcaaga ttgattcagg gatgggacgg attggtttta
    71761 gagaggccag tgaggttgag caggctcaag atttgctcca acaacacggt gttcgtgttg
    71821 aaggaatctt tacccacttt gctactgctg atgaggaatc agatgactat tttaatgccc
    71881 agttagaacg gtttaaaact attttagcta gtatgaagga agttccagag ctggttcatg
    71941 ctagcaattc tgcaacgact ctttggcatg tagagactat tttcaatgcg gttcgtatgg
    72001 gagatgccat gtatggcctc aatccaagtg gagcggtctt ggatttgcct tatgatttga
    72061 taccggcctt gaccttggag tctgctctgg ttcatgtcaa gacagttcca tctggagctt
    72121 gcatgggata tggagcaact tatcaagcgg atagcgagca agtcatcgcg accgtgccaa
    72181 tcgggtatgc agatggatgg acaagagaca tgcaaaattt ctctgtcttg gtagatggcc
    72241 aagcttgccc aattgtcggc agggtttcga tggaccaaat cactattcga ttgcctaagc
    72301 cttacccact cggaaccaag gtaaccttga ttggctccaa tggggataag gaaatcactg
    72361 caactcaggt agcgacctac cgcgtaacca ttaactatga ggtggtttgc ctcctcagcg
    72421 accgtattcc gagagaatat tattagaaaa gaaaggagtg gagcatgaat ctacatcaac
    72481 ccttgcatgt cttgcctggt gtgggaccaa agtcagcaga aaaatacgcc aaactaggaa
    72541 ttgaaaactt gcaagatctc ttgctctact ttcctttccg ttatgaagac ttcaaaacca
    72601 agcaggtgct ggagctggaa gacggtgaga aggcagttct ttctggtcag gtagtgactc
    72661 ctgctagtgt ccagtattat ggtttcaagc gcaatcgcct gcgttttagt ctcaagcagg
    72721 gagaggtcgt ttttgcggtg aatttcttta accagcccta tctggctgat aaaatagagt
    72781 tgggagcaac ccttgctgtc tttggaaaat gggaccgcgc taaggctagt ctgactggga
    72841 tgaaggttct ggctcaggta gaagatgacc tccagcctgt ttatcgtctg gctcagggaa
    72901 tcagtcaggc cagtctggtt aaggtcatca agacggcttt tgatcaggga ctggacctct
    72961 tgatagaaga aaatctgccc cagtctttac tagacaaata caaactcatg tcccgttgtc
    73021 aggcagtccg agctatgcat tttccaaagg atttggcaga atacaagcag gctcttcgcc
    73081 gtataaagtt tgaggaactc ttttatttcc aaatgcagct gcagacgctc aagtctgaaa
    73141 atagaattca gggaagtggt ctggttctgg attggtctca ggaaaaagtg acagcagtta
    73201 aagcaagtct tccttttgcc ctgacccaag ctcaggaaaa gagtttgcag gaaattttaa
    73261 ctgatatgaa gtccgaccac cacatgaatc gtctcctaca aggggatgtg gggagtggaa
    73321 aaacggtagt cgctggcttg gccatgtttg cggcagtgac agcaggttat caggctgccc
    73381 taatggtacc aacagaaatc ctcgcagagc aacactttga gagtttacag aacctttttc
    73441 ccaatttgaa actggctctc ttgacaggtt ccttgaaagc tgcagaaaag agagaagtct
    73501 tggagaccat tgccaagggt gaggctgatt tgattatagg aactcacgct ctgatacaag
    73561 atggggtgga gtatgctcgt cttggtttga ttattatcga tgagcagcac cgttttggtg
    73621 tagggcaaag gcgtatttta cgggaaaaag gtgacaatcc agatgtcctc atgatgacgg
    73681 cgactcccat tccacggacg cttgccatca cagcctttgg agatatggat gtttccatta
    73741 tcgaccagat gccagcaggt cggaagccta ttgtgacgcg ctggatcaaa catgagcaac
    73801 tacctcaggt cttgacttgg ttagaggggg aaattcaaaa aggttcccaa gcctatgtca
    73861 tctctccttt gattgaagaa tcagaagctc tagatttgaa aaatgccatt gccttatcag
    73921 aagagctgac ggctcatttt gcaggcaagg cagaggtggc tcttctacat ggtaggatga
    73981 agagtgacga aaaagaccag atcatgcagg atttcaagga gagaaagacg gatattctgg
    74041 tttcgacgac ggttattgag gttggggtca acgttcccaa tgcgactgtc atgattatca
    74101 tggatgccga tcgcttcggg ctcagccagc ttcaccagct tagaggtcgt gtcggtcggg
    74161 gggacaagca gtcctacgct gttctcgttg ccaatcccaa gacggattct gggaaagacc
    74221 gcatgcgcat catgacagaa acgaccaatg gatttgtcct tgcggaggaa gatttgaaaa
    74281 tgcgtggttc tggtgagatt tttggaacca gacagtcagg acttccagag ttccaagtgg
    74341 ctgatattat cgaagatttt ccgattttag aagaagcaag aaaggttgct agctacatta
    74401 gttctataga agcttggcaa gaagatccag agtggcgcat gattgccctt catctggaaa
    74461 agaaagaaca tctggattaa gctttctcta aggaaaactt aagctagttt ttaggtttgg
    74521 ctcttatact aaagtcatca aaaagaaacg aggactctca tatgacagta acgattaaag
    74581 taaattacca aaccactttc caaaagaagg aagcaaaaaa ctagtataaa cagaagagag
    74641 agcgaaatgc tctttttcgt ttctaaaact actttcagcc catcatccta aaagtaaaga
    74701 atctaaattc actttctatt tatccttctt tcttgcattg attacataga tatgctacag
    74761 ttgtggtaac gattacaaaa taaaaggagc atgctatgaa aaatccagct ttgctagaag
    74821 aaattaagac ctatagagga agggatgagg ttccggaaga ctttgatgct ttctgggatg
    74881 gggaagtgaa aaatgtttcc acgcttccat cctaccactt ggaggaaaga gatttccaca
    74941 ttcctcaagt caagtgctat gagttaacat ttgaaggaag caaggaagga aaggtctatg
    75001 cacgcattgt tcttccaaag agtgaggaga aggttccact aatcttccat tttcatggtt
    75061 atatgggacg tggctgggac tgggccgaca tgctgggctt caccgtagct ggttacggtg
    75121 ttgtttccat ggatgtgcgg ggccagtcag gttactcaca agacggcttg cgttctcctt
    75181 taggaaatac cgtgaagggg catattatcc gtggtgctat ggaaggtcgg gaccacctct
    75241 tttataagga tgtttatctg gatatttacc agttggtcga aattgttgct agtctgtctc
    75301 aggttgatga gaagcgtctt tctagctatg gtgcctcaca aggaggggct ctagctctag
    75361 ttgcagcagc gctcaatcct cgaattcaga aaacagttgc catttatccc ttcttgtcag
    75421 acttcagacg ggtgattgag attggtaata ctagcgaggc ttacgacgaa cttttccgct
    75481 atttcaagtt tcacgacccc ttccatgaaa cagaggagga aatcatggcg acccttgcct
    75541 atatcgatgt caaaaatctt gcccatcgta tccaaggtga ggttaagatg attacgggct
    75601 tggacgacga tgtttgctat cccattaccc agtttgcgat ttataatcgt ctgacctgcg
    75661 ataaaaccta tcgcatcatg cctgagtatg ctcacgaagc catgaatgta tttgtcaatg
    75721 accaagtcta caactggctc tgtggaagtg agattccttt taaatatcta aaataaggag
    75781 tcgactctaa gcacaaaatc ttaaaaatta caaacacgca tagtatcagg ggattaaaaa
    75841 aacttgatac tatgcgtttt atcatggaaa tatagtaaaa tgaaacaaga ataggacaaa
    75901 acgatcagga cagtcaaatc gatttctaac aatgttttag aagcaaatgt gtactattct
    75961 agtttcaatc tattatattt atagaatttt ttgttgctag atttgtcaaa ttgcttaaaa
    76021 taattttttt cagaaagcaa aagccgatac ctatcgagta gggtagttct tgctatcgtc
    76081 aggcttgtct gtaggtgtta atacttttca aaaatctctt taaaccacgt cagcttcgcc
    76141 ttgccgtata tatgttactg acttcgtcag ttctatctgc cacctcaaaa cggtgttttg
    76201 agctgacttc gtcagttcta tctgccacct caaaacggtg ttttgagctg actttgtcag
    76261 tcttatctac aacctcaaaa cagtgttttg agcatcatgc ggctagcttc ttagtttgct
    76321 ctttgatttt cattgagtat aaaaacagat gagtttctgt tttcttttta tggactataa
    76381 atgttcagct gaaactactt tcaaggacat tattatataa aagaattttt tgaaactaaa
    76441 atctactata ttacactata ttgaaagcgt tttaaaaatg aggtataata aatttactaa
    76501 cgcttataaa aagtgataga atctattttt atgtatatgt aaagatagat tgctgtaaaa
    76561 atagtagtag ctatgcgaaa taacagatag agagaaggga ttgaagctta gaaaagggga
    76621 ataatatgat atttaaggca ttcaagacaa aaaagcagag aaaaagacaa gttgaactac
    76681 ttttgacagt ttttttcgac agttttctga ttgatttatt tcttcactta tttgggattg
    76741 tcccctttaa gctggataag attctgattg tgagcttgat tatatttccc attatttcta
    76801 caagtattta tgcttatgaa aagctatttg aaaaagtgtt cgataaggat tgagcaggaa
    76861 gtatggtgta aatagcatag gctgatgtcc atcatttgct tataaagaga tattttagtt
    76921 taattgcagc ggtgtcctgg tagataaact agattggcag gagtctgatt ggagaaagga
    76981 gaggggaaaa ttggcaccaa tttgagatag tttgtttagt tcatttttgt catttaaatg
    77041 aactgtagta aaagaaagtt aataaaagac aaactaagtg cattttctgg agtaaatgtc
    77101 ttatttcaga aatcgggata tagatataga gaggatcagt atgaatcgga gtgttcaaga
    77161 acgtaagtgt cgttatagca ttaggaaact atcggtagga gcggtttcta tgattgtagg
    77221 agcagtggta tttggaacgt ctcctgtttt agctcaagaa ggggcaagtg agcaacctct
    77281 ggcaaatgaa actcaacttt cgggggagag ctcaacccta actgatacag aaaagagcca
    77341 gccttcttca gagactgaac tttctggcaa taatcaagaa caagaaagga aagataagca
    77401 agaagaaaaa attccaagag attactatgc acgagatttg gaaaatgtcg aaacagtgat
    77461 agaaaaagaa gatgttgaaa ccaatgcttc aaatggtcag agagttgatt tatcaagtga
    77521 actagataaa ctaaagaaac ttgaaaacgc aacagttcac atggagttta agccagatgc
    77581 caaggctcca gcattctata atctcttttc tgtgtcaagt gctactaaaa aagatgagta
    77641 cttcactatg gcagtttaca ataatactgc tactctagag gggcgtggtt cggatgggaa
    77701 acagttttac aataattaca acgatgcacc cttaaaagtt aaaccaggtc agtggaattc
    77761 tgtgactttc acagttgaaa aaccgacagc agaattgcca aatggtcgag ttcgcctgta
    77821 tgttaatggt gttttatctc gaacaagtct gaaatctggc aatttcatta aagatatgcc
    77881 agatgtaacg catgtgcaaa tcggagcaac caagcgtgcc aacaatacgg tttgggggtc
    77941 aaatctacag attcggaatc tcactgtgta taatcgtgct ttaacaccag aagaggtaca
    78001 aaaacgtagt caacttttta aacgctcaga tttagaaaaa aaactacctg aaggagcggc
    78061 tttaacagag aaaacggaca tattcgaaag cgggcgtaac ggtaaaccaa ataaagatgg
    78121 aatcaagagt tatcgtattc cagcacttct caagacagat aaaggaactt tgatcgcagg
    78181 tgcagatgaa cgccgtctcc attcgagtga ctggggtgat atcggtatgg tcatcagacg
    78241 tagtgaagat aatggtaaaa cttggggtga ccgagtaacc attaccaact tacgtgacaa
    78301 tccaaaagct tctgacccat cgatcggttc accagtgaat atcgatatgg tgttggttca
    78361 agatcctgaa accaaacgaa tcttttctat ctatgacatg ttcccagaag ggaagggaat
    78421 ctttggaatg tcttcacaaa aagaagaagc ctacaaaaaa atcgatggaa aaacctatca
    78481 aatcctctat cgtgaaggag aaaagggagc ttataccatt cgagaaaatg gtactgtcta
    78541 tacaccagat ggtaaggcga cagactatcg cgttgttgta gatcctgtta aaccagccta
    78601 tagcgacaag ggtgatctat acaagggtaa ccaattacta ggcaatatct acttcacaac
    78661 aaacaaaact tctccattta gaattgccaa ggatagctat ctatggatgt cctacagtga
    78721 tgacgacggg aagacatggt cagctcctca agatattact ccgatggtca aagccgattg
    78781 gatgaaattc ttgggtgtag gtcctggaac aggaattgta cttcggaatg ggcctcacaa
    78841 gggacggatt ttgataccgg tttatacgac taataatgta tctcacttaa atggctcgca
    78901 atcttctcgt gtcatctatt cagatgatca tggaaaaact tggcatgctg gagaagcggt
    78961 caacgataac cgtcaggtag acggtcaaaa gatccactct tctacgatga acaataaacg
    79021 tgcgcaaaat acagaatcaa cggtggtaca actaaacaat ggagatgtta aactctttat
    79081 gcgtggtttg actggagatc ttcaggttgc tacaagtaaa gacggaggag tgacttggga
    79141 gaaggatatc aaacgttatc cacaggttaa agatgtctat gttcaaatgt ctgctatcca
    79201 tacgatgcat aatggaaaag aatacattat cctcagcaat gcaggtggac cgaatcgtga
    79261 aaatgggatg gtccacttgg cacgtgtcga agaaaatggt gagttgactt ggctcaaaca
    79321 caatccaatt caaaaaggag agtttgccta taattcgctc caagaattag gaaatgggga
    79381 gtatggcatc ttgtatgaac atactgaaaa aggacaaaat gcctataccc tatcatttag
    79441 aaaatttaat tgggaatttt tgagcaaaga tctgatttct cctaccgaag cgaaagtgaa
    79501 gcgaactaga gagatgggca aaggagagat gggcaaagga gttattggct tggagtttga
    79561 ctcagaagta ttggtcaaca aggctccaac ccttcaattg gcaaatggta aaacagcgac
    79621 tttcctaacc cagtatgata gcaaaacctt gttgtttgca gtagataagg aagatatcgg
    79681 acaggaaatt attggtatag ctaaaggaag catcgaaagt atgcataatc ttcctgtaaa
    79741 tctagcaggt gccagagttc ctggcggagt aaatggtagc aaagcagcgg tgcatgaagt
    79801 tccagaattt acagggggag ttaatggtac agagccagct gttcatgaaa tcgcagagta
    79861 taagggatct gattcgcttg taactcttac tacaaaagaa gattatactt acaaagctcc
    79921 tcttgctcag caggcacttc ctgaaacagg aaacaaggag agtgacctcc tagcttcact
    79981 aggactaaca gctttcttcc ttggtctgtt tacgctaggg aaaaagagag aacaataaga
    80041 gaagaattct aaacatttga ttttgtaaaa atagaaggag atagcaggtt ttcaagcctg
    80101 ctatcttttt ttgatgacat tcaagctgat acgaaatcat aagaggtctg aaactacttt
    80161 cagagtagtc tgttctataa aatatagta
//
LOCUS       DBERDK010000002        64279 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.002, whole genome shotgun sequence.
ACCESSION   DBERDK010000002 DBERDK010000000
VERSION     DBERDK010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 64279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64279
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.002"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..206
                     /locus_tag="ACL73L_000080"
     CDS             <1..206
                     /locus_tag="ACL73L_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGS0771138.1"
                     /translation="SIYPKEKISYFYGISQTSYFIGDTLGGFIGGWLFNQDMKYSIYL
                     FSVLLLFNAVLFNRFYLRNNKNS"
     gene            complement(340..1397)
                     /locus_tag="ACL73L_000081"
                     /pseudo
     CDS             complement(340..1397)
                     /locus_tag="ACL73L_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681261.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1968..3917
                     /gene="mutL"
                     /locus_tag="ACL73L_000082"
     CDS             1968..3917
                     /gene="mutL"
                     /locus_tag="ACL73L_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HGS0771139.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKVQITDNGYGISHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLVSA
                     KKMIEIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGYGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMTLVSEAIANSLKEQTLIPD
                     ALENLAKSTVRNREKVEQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAKETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            complement(4242..4709)
                     /gene="ribE"
                     /locus_tag="ACL73L_000083"
     CDS             complement(4242..4709)
                     /gene="ribE"
                     /locus_tag="ACL73L_000083"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HGS0771140.1"
                     /translation="MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVN
                     EKDIEVAWVPGAFEIPLIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQ
                     ISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(4710..5915)
                     /locus_tag="ACL73L_000084"
     CDS             complement(4710..5915)
                     /locus_tag="ACL73L_000084"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HGS0771141.1"
                     /translation="MEYRKIQEALEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERNGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRTDDLIQFSKKHNIPLITIKELQEYRKVYDQLVERVSTVNMPTRYG
                     NFKAISYIDKLNGEHHLALIMGNIEDEANVLCRVHSECLTGDVLGSLRCDCGQQFDKA
                     MKMIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAQMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYDSFYLETKKNRM
                     GHILNMEEK"
     gene            complement(5935..6570)
                     /gene="ribE"
                     /locus_tag="ACL73L_000085"
     CDS             complement(5935..6570)
                     /gene="ribE"
                     /locus_tag="ACL73L_000085"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HGS0771142.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSVNGRLGGHVVTGHI
                     DGTGKISSIKKDDNAVWYQINTQKEILDLIVEKGSITIDGISLTVAKVSKVNFSVSVI
                     PHTLEQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKELLYQNGF"
     gene            complement(6555..7655)
                     /gene="ribD"
                     /locus_tag="ACL73L_000086"
     CDS             complement(6555..7655)
                     /gene="ribD"
                     /locus_tag="ACL73L_000086"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HGS0771143.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAVIVKDNHIIGQGYHE
                     FFGGPHAERNALKNCRESPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGSLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMSMDGK
                     IATKTNQSKWITEEEARKHVHQLRHYVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCEILSIKKKGNHIDLSS
                     LMQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKIFGGSAKFPVGGEGISL
                     PNDAIRLKPYAFSQIGNDYLIESEVIYPCSQE"
     regulatory      complement(7790..7904)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            8061..8654
                     /gene="ruvA"
                     /locus_tag="ACL73L_000087"
     CDS             8061..8654
                     /gene="ruvA"
                     /locus_tag="ACL73L_000087"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HGS0771144.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQEA
                     QIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDGLPAKVAVQASAENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     gene            8664..9227
                     /locus_tag="ACL73L_000088"
     CDS             8664..9227
                     /locus_tag="ACL73L_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HGS0771145.1"
                     /translation="MTKRCSWVKMTNPLYIAYHDEEWGQPLHDDQALFELLCMETYQA
                     GLSWETVLNKRQAFREAFHSYQIHSVAEMTETELEAMLENPAIIRNRAKLFATRANAQ
                     AFLQLQAEYGSFDAYLWSFVEGKTVVNDVPDYRQAPAKTPLSEKLAKDLKKRGFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWRGLK"
     gene            9224..9901
                     /locus_tag="ACL73L_000089"
     CDS             9224..9901
                     /locus_tag="ACL73L_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HGS0771146.1"
                     /translation="MMSNKNKGILIFAILYTVLFMFDGVKLLASLMPSAIANYLVYVV
                     LALYGSFLFKDRLIQQWKEIRKTKRKFFFGVLTGWLFLILMTVVFEFVSEMLKQFVGL
                     DGQGLNQSNIQSTFQEQPLLIAVFACVIGPLVEELFFRQVLLHYLQERLPGLLSIILV
                     GLVFALTHMHSLALSEWIGAVGYLGGGLAFSIIYVKEKENIYYPLLVHMLSNSLSLII
                     LAISIVK"
     gene            complement(10056..11087)
                     /locus_tag="ACL73L_000090"
     CDS             complement(10056..11087)
                     /locus_tag="ACL73L_000090"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HGS0771147.1"
                     /translation="MVSTIGIVSLSSGIIGEDFVKHEVDLGIQRLKDLGLNPIFLPHS
                     LKGLDFIKDHPEARAEDLIHAFSDDSIDMILCAIGGDDTYRLLPYLFENDQLQKVIKQ
                     KIFLGFSDTTMNHLMLHKLGIKTFYGQSFLADICELDKEMLAYSLHYFKELIETGRIS
                     EIRPSDVWYEERTDFSPTALGTPRVSHTNTGFDLLQGRAQFEGKILGGCLESLYDIFD
                     NSRYADSTELCQKYKLFPDLSDWEGKILLLETSEEKPKPEDFKKMLLTLKDTGIFAVI
                     NGLLVGKPMDETFHDDYKEALLDIIDSNIPIVYNLNVGHATPRAIVPFGVHAHVDAQE
                     QVIRFDYNK"
     gene            complement(11342..12025)
                     /locus_tag="ACL73L_000091"
     CDS             complement(11342..12025)
                     /locus_tag="ACL73L_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HGS0771148.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPQ
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMDSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGPDMDRSQRPPFWK
                     SVLVILASMFLWLLVFFATSFLPASLNPVLDPLPLAIIGAALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
     gene            complement(12037..12981)
                     /locus_tag="ACL73L_000092"
     CDS             complement(12037..12981)
                     /locus_tag="ACL73L_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HGS0771149.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRTYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            13113..15944
                     /gene="uvrA"
                     /locus_tag="ACL73L_000093"
     CDS             13113..15944
                     /gene="uvrA"
                     /locus_tag="ACL73L_000093"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HGS0771150.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKPDVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAINASSVEQIVDKVLELPERQRLQILAPVIRKKK
                     GQHKSVIEKVQKDGYVRVRVDGEVYDVTEVPELSKSKQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALRIAEGYVIIDTMDDSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPDASKTLHEGALAPWNPISSNYYPNMLEQAMKVFGVAMDKP
                     FEDLSEEDKNLILYGSDGKEFHFHYENEFGGVRDIDIPFEGVINNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCQGYRLNDQALSVRVGGQQGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVTGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINSILKKAIAQKLNRNSDKPGKFKTITGIEHV
                     DRLIDINQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDAGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHE"
     gene            15937..16998
                     /locus_tag="ACL73L_000094"
     CDS             15937..16998
                     /locus_tag="ACL73L_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HGS0771151.1"
                     /translation="MNKRVQAFLAKMQEKELDGIIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIAGIVKDMNLTRIGFEDEISVSYY
                     HRMQAAFAGLNLLPQTQFVEGLRMIKDEAEIAAIRKACSISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASGLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKVGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMALTDEPGIYIEGKYGVRIEDDIL
                     ITETGCELLTLAPKELIVI"
     gene            17130..17528
                     /gene="spx"
                     /locus_tag="ACL73L_000095"
     CDS             17130..17528
                     /gene="spx"
                     /locus_tag="ACL73L_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HGS0771152.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
     gene            17594..18163
                     /locus_tag="ACL73L_000096"
     CDS             17594..18163
                     /locus_tag="ACL73L_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP0191 family lipoprotein"
                     /protein_id="HGS0771153.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGPATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPKSDDGSQQTQTITYKDKTFLSLTIQQKRPVSDELKTYIDQHGVE
                     ETQKALLEAEEKDKSIIEARKLAGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGLENLLKNEPSKYISDRLANGATEQ"
     gene            18250..18516
                     /locus_tag="ACL73L_000097"
     CDS             18250..18516
                     /locus_tag="ACL73L_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HGS0771154.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            18520..18939
                     /gene="ruvX"
                     /locus_tag="ACL73L_000098"
     CDS             18520..18939
                     /gene="ruvX"
                     /locus_tag="ACL73L_000098"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HGS0771155.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINEEQGKFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPVDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            18955..19260
                     /locus_tag="ACL73L_000099"
     CDS             18955..19260
                     /locus_tag="ACL73L_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HGS0771156.1"
                     /translation="MSHDHNHDHEERELITLVDEQGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDSEDEWNMIEEVFNSFMEE"
     gene            19618..20868
                     /locus_tag="ACL73L_000100"
     CDS             19618..20868
                     /locus_tag="ACL73L_000100"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HGS0771157.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRMQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLADQVKE
                     MEKTLLQTPDQLSFFELLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLICQKKAESLAVNLYQA
                     GQDFLMLNGDFSSSLLNLSQLDIGLEGVYQQENAALALQTFLLFMGEGKEAVDEQAVR
                     KALEQTHWAGRLERIRPQIYLDGAHNLPALTRLVEFIKEKEQEGYRPQILFGSLKRKD
                     YQGMLGYLTENLPQVELKMTGFDYQGSLDETDVTGYHVIPSYREFISSFEERADAQDL
                     LFVTGSLYFISEVRGYLLDREQIN"
     gene            20952..21383
                     /locus_tag="ACL73L_000101"
     CDS             20952..21383
                     /locus_tag="ACL73L_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0198 family lipoprotein"
                     /protein_id="HGS0771158.1"
                     /translation="MKLKRFTLSLASLASFSLLVACSQRAQQVQQPAQQNTNTANAGG
                     NQNQAAPVQNQPVAQPTDIDGTYTGQDDGDRITLVVTGTTGTWTELESDGDQKVKQVT
                     FDSANQRMIIGDDVKIYTVNGNQIVVDDMDRDPSDQIVLTK"
     gene            21529..23061
                     /gene="cls"
                     /locus_tag="ACL73L_000102"
     CDS             21529..23061
                     /gene="cls"
                     /locus_tag="ACL73L_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HGS0771159.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPYIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTPSIREIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
     gene            23139..23282
                     /locus_tag="ACL73L_000103"
     CDS             23139..23282
                     /locus_tag="ACL73L_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771160.1"
                     /translation="MLTKEEVNDMIEFKQTHLHRFREIETFVKLCKKSLKQPSQADNP
                     RTF"
     gene            23319..24857
                     /locus_tag="ACL73L_000104"
     CDS             23319..24857
                     /locus_tag="ACL73L_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HGS0771161.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHHRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWALHQTGKIYSDYYGSQGLLYYLLTYVSQGG
                     FFFAIFEWLALVAGGFFLFRSADTLTEQGDQAGHLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSHDKGFVRIGLALAGGFFFAPLSSLLFIAVVSLGLLVFNLG
                     HRRFVHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLSLGLGFVVCIFLGLFQSKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMVVFPVFLLLLVTNIKSQQRGRSARRSRRETPVSLWSRFFKGNLYLLVFGFVYLL
                     SVPFLMKFVLYPVPYQERNRLADLVKEETNTEDAIYAWDDTATLYRKSERLSPSAILS
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVVVWSEVETLLKENYQQVKTDYSEFKV
                     YKIK"
     gene            24977..27184
                     /gene="nrdD"
                     /locus_tag="ACL73L_000105"
     CDS             24977..27184
                     /gene="nrdD"
                     /locus_tag="ACL73L_000105"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HGS0771162.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALHKAADKVMD
                     VTPLVEKRLNDLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLVDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKREDYAWK
                     KTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKDENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHRLGKEESVDQLFKNRRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSDQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKTVDVVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQVTN"
     gene            27196..27336
                     /locus_tag="ACL73L_000106"
     CDS             27196..27336
                     /locus_tag="ACL73L_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771163.1"
                     /translation="MGKYQLDDKGRAQVTRYHEKYSKGGAGKKERLLSFREQFLNKNK
                     KK"
     gene            27380..27886
                     /locus_tag="ACL73L_000107"
     CDS             27380..27886
                     /locus_tag="ACL73L_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS0771164.1"
                     /translation="MELRRPRLADKKAVLDMMTEFEKSQSAHDGGFWDTENFVYEEWL
                     ESNQEQEMGINLPEGWVPAIQLVAFSEKGQAVGFLNLRLRLSNFLLEEGGHIGYSIRP
                     SERGKGYAKETLRQGLQVAKEKNIKKALVTCSVNNPASRAVILANGGIFEDAHNGVER
                     YWIEVANE"
     gene            27879..28469
                     /gene="nrdG"
                     /locus_tag="ACL73L_000108"
     CDS             27879..28469
                     /gene="nrdG"
                     /locus_tag="ACL73L_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HGS0771165.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVSGCMF
                     HCEGCYNVATWSFNAGIPYTAELEEQIMADLAQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELSDKDIWSWTGYTWEEMMLETPDKLELLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKESYEQVKRE"
     gene            28466..29083
                     /locus_tag="ACL73L_000109"
     CDS             28466..29083
                     /locus_tag="ACL73L_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HGS0771166.1"
                     /translation="MKKKDLVDQLVSEIETGKVRTLGIYGHGASGKSTSAQELYQALD
                     STTVNLLETDPYITSGRHLVVPKDAPNQKVTAGLPVAHELESLQRDILALQASMDVLT
                     IEEPWKASEVLSGAKPILIVEGMSVGFLPKELFEKTICFYTDEETELKRRLARDTTVR
                     NRDASFILASHQMRREQYLRYYKETESKADILVDQSEDKFDVKRT"
     gene            29349..29657
                     /gene="rpsJ"
                     /locus_tag="ACL73L_000110"
     CDS             29349..29657
                     /gene="rpsJ"
                     /locus_tag="ACL73L_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HGS0771167.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            29874..30500
                     /gene="rplC"
                     /locus_tag="ACL73L_000111"
     CDS             29874..30500
                     /gene="rplC"
                     /locus_tag="ACL73L_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HGS0771168.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            30525..31148
                     /gene="rplD"
                     /locus_tag="ACL73L_000112"
     CDS             30525..31148
                     /gene="rplD"
                     /locus_tag="ACL73L_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HGS0771169.1"
                     /translation="MANVTLFDQTGKEAGQVVLNDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQAAISKIEEVLA"
     gene            31148..31444
                     /locus_tag="ACL73L_000113"
     CDS             31148..31444
                     /locus_tag="ACL73L_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HGS0771170.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            31462..32295
                     /gene="rplB"
                     /locus_tag="ACL73L_000114"
     CDS             31462..32295
                     /gene="rplB"
                     /locus_tag="ACL73L_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HGS0771171.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            32399..32680
                     /gene="rpsS"
                     /locus_tag="ACL73L_000115"
     CDS             32399..32680
                     /gene="rpsS"
                     /locus_tag="ACL73L_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HGS0771172.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            32692..33036
                     /gene="rplV"
                     /locus_tag="ACL73L_000116"
     CDS             32692..33036
                     /gene="rplV"
                     /locus_tag="ACL73L_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HGS0771173.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIAILTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            33049..33702
                     /gene="rpsC"
                     /locus_tag="ACL73L_000117"
     CDS             33049..33702
                     /gene="rpsC"
                     /locus_tag="ACL73L_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HGS0771174.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDALRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            33706..34119
                     /gene="rplP"
                     /locus_tag="ACL73L_000118"
     CDS             33706..34119
                     /gene="rplP"
                     /locus_tag="ACL73L_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HGS0771175.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            34129..34335
                     /gene="rpmC"
                     /locus_tag="ACL73L_000119"
     CDS             34129..34335
                     /gene="rpmC"
                     /locus_tag="ACL73L_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HGS0771176.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTARLKEVKKQIARIKTVQSEAK"
     gene            34360..34620
                     /gene="rpsQ"
                     /locus_tag="ACL73L_000120"
     CDS             34360..34620
                     /gene="rpsQ"
                     /locus_tag="ACL73L_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HGS0771177.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            34646..35014
                     /gene="rplN"
                     /locus_tag="ACL73L_000121"
     CDS             34646..35014
                     /gene="rplN"
                     /locus_tag="ACL73L_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HGS0771178.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            35092..35397
                     /gene="rplX"
                     /locus_tag="ACL73L_000122"
     CDS             35092..35397
                     /gene="rplX"
                     /locus_tag="ACL73L_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HGS0771179.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIIEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            35421..35963
                     /gene="rplE"
                     /locus_tag="ACL73L_000123"
     CDS             35421..35963
                     /gene="rplE"
                     /locus_tag="ACL73L_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HGS0771180.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNAKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            35981..36250
                     /gene="rpsN"
                     /locus_tag="ACL73L_000124"
     CDS             35981..36250
                     /gene="rpsN"
                     /locus_tag="ACL73L_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HGS0771181.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            36535..36933
                     /gene="rpsH"
                     /locus_tag="ACL73L_000125"
     CDS             36535..36933
                     /gene="rpsH"
                     /locus_tag="ACL73L_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HGS0771182.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGPNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            37125..37661
                     /gene="rplF"
                     /locus_tag="ACL73L_000126"
     CDS             37125..37661
                     /gene="rplF"
                     /locus_tag="ACL73L_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HGS0771183.1"
                     /translation="MSRIGNKVIVLPAGVELANNDNVVTVKGPKGELTREFSKDIEIR
                     VEGTEVTLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            37745..38101
                     /gene="rplR"
                     /locus_tag="ACL73L_000127"
     CDS             37745..38101
                     /gene="rplR"
                     /locus_tag="ACL73L_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HGS0771184.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            38119..38613
                     /gene="rpsE"
                     /locus_tag="ACL73L_000128"
     CDS             38119..38613
                     /gene="rpsE"
                     /locus_tag="ACL73L_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HGS0771185.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            38627..38809
                     /gene="rpmD"
                     /locus_tag="ACL73L_000129"
     CDS             38627..38809
                     /gene="rpmD"
                     /locus_tag="ACL73L_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HGS0771186.1"
                     /translation="MAQIKITLTKSPIGRIPSQRKTVVALGLGKLNSSVIKEDNAAIR
                     GMITAVSHLVTVEEVN"
     gene            38954..39394
                     /gene="rplO"
                     /locus_tag="ACL73L_000130"
     CDS             38954..39394
                     /gene="rplO"
                     /locus_tag="ACL73L_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HGS0771187.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            39407..40717
                     /gene="secY"
                     /locus_tag="ACL73L_000131"
     CDS             39407..40717
                     /gene="secY"
                     /locus_tag="ACL73L_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HGS0771188.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDRTE"
     gene            40868..41506
                     /locus_tag="ACL73L_000132"
     CDS             40868..41506
                     /locus_tag="ACL73L_000132"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HGS0771189.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAHALDKTLA
                     ELGIELEGVINIEVNPDSLLERLSGRIIHRVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQNINDVFSDIEKVLTNLK"
     gene            41623..41841
                     /gene="infA"
                     /locus_tag="ACL73L_000133"
     CDS             41623..41841
                     /gene="infA"
                     /locus_tag="ACL73L_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HGS0771190.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
     gene            41866..41982
                     /gene="rpmJ"
                     /locus_tag="ACL73L_000134"
     CDS             41866..41982
                     /gene="rpmJ"
                     /locus_tag="ACL73L_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HGS0771191.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            42000..42365
                     /gene="rpsM"
                     /locus_tag="ACL73L_000135"
     CDS             42000..42365
                     /gene="rpsM"
                     /locus_tag="ACL73L_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HGS0771192.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            42383..42766
                     /gene="rpsK"
                     /locus_tag="ACL73L_000136"
     CDS             42383..42766
                     /gene="rpsK"
                     /locus_tag="ACL73L_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HGS0771193.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            42809..43744
                     /locus_tag="ACL73L_000137"
     CDS             42809..43744
                     /locus_tag="ACL73L_000137"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HGS0771194.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            43756..44142
                     /gene="rplQ"
                     /locus_tag="ACL73L_000138"
     CDS             43756..44142
                     /gene="rplQ"
                     /locus_tag="ACL73L_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HGS0771195.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            44407..44673
                     /locus_tag="ACL73L_000139"
     CDS             44407..44673
                     /locus_tag="ACL73L_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HGS0771196.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAVVSSDEKQDFTYLRNEFEAFGQTLNVKINIQSAAIFEAMYNI"
     gene            44683..46020
                     /locus_tag="ACL73L_000140"
     CDS             44683..46020
                     /locus_tag="ACL73L_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HGS0771197.1"
                     /translation="MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPDINRAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDATDYVVLAKALDKA
                     AKEIGVDFIGGFSALVQKGYQKGDEILINSIPRALAETDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGVAKLVVFANAVEDNPFMEGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALALLNDQVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGKEGDMIEFGGLLGTAPVMKVNGASSVDFISRGGQIPAPIHSFKN"
     gene            46264..46956
                     /locus_tag="ACL73L_000141"
     CDS             46264..46956
                     /locus_tag="ACL73L_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HGS0771198.1"
                     /translation="MVKVRLYLVRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGKIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEIIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLINGMHPHGLDNGSVTILEYEDGQFRVEVVGDRSYR
                     ELGREKMEEGSI"
     gene            complement(47059..48711)
                     /locus_tag="ACL73L_000142"
                     /pseudo
     CDS             complement(47059..48711)
                     /locus_tag="ACL73L_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(48701..49791)
                     /locus_tag="ACL73L_000143"
                     /pseudo
     CDS             complement(48701..49791)
                     /locus_tag="ACL73L_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289361.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(49804..50925)
                     /locus_tag="ACL73L_000144"
                     /pseudo
     CDS             complement(49804..50925)
                     /locus_tag="ACL73L_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            complement(51512..52288)
                     /locus_tag="ACL73L_000145"
     CDS             complement(51512..52288)
                     /locus_tag="ACL73L_000145"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HGS0771199.1"
                     /translation="MEISKGIIFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCSNPESQ
                     RMKPEKMKDAQREKFTLVGEEKTVEEIITEVLKDKEFYEESGGGLTLSGGEIFAQFEF
                     AKAILKSAKEHHIHTAIETTAFVDHEKFIDLIQYVDFIYTDLKHYNSIKHKKVTGVFN
                     QMIIKNIHYAFSQNKTIVLRIPVIPNFNNSLEDAEKFATLFNSLNIDQVQLLPFHQFG
                     ENKYRLLNRKYEMDGINALHPEDLIDYQKVFLNHHINCYF"
     gene            52410..53156
                     /locus_tag="ACL73L_000146"
     CDS             52410..53156
                     /locus_tag="ACL73L_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGS0771200.1"
                     /translation="MKRLEQIIKLVSEYEKIDVNTLSEKLNVSKVTIRKDLDKLESKG
                     LLHREHGYAVLNSGDDLNVRLSINYEIKRKIVQEAVKLVSDNETIMIESGSTCALLAE
                     EICKQKRNVTIVTNSFFIANFVRAYDSCRIIVLGGEFQKDSQVTVGPLLKEMIQTFHV
                     HQAFVGTDGYDKEMGFTGKDLMRSEVVQYISAASDKVIVLTDSSKFDKRGTVRRFALS
                     QVYEVITDEKLSKQNIATLENAGIMVKVVS"
     gene            53169..54149
                     /locus_tag="ACL73L_000147"
     CDS             53169..54149
                     /locus_tag="ACL73L_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HGS0771201.1"
                     /translation="MNQDRNKLLSKIAYLYYIENLNQSQIAAKLGIYRTSISRMLTEA
                     RNAGIVKIEIENFDTNMFKLENYVKEKYGLESLEIIPNEFDDNPTILSERISQVAAGV
                     LRNLIDDNMKIGFSWGKSLSNLVDLIHSKSVRNVHFYPLAGGPSHIHAKYHVNTLIYE
                     MSRKFHGECTFMNATIVQENKLLADGILQSRYFENLKNSWKDLDIAVVGIGDFSNKGK
                     HQWLDMLTEDDFKELTKVKTVGEICCRFFDSKGKEVYENLQERTIAISLEDLKNIPQS
                     LAVAYGDTKVSSILSVLRANLVNHLITDKNTILKVLEEDGDLTFREILGE"
     gene            54344..54664
                     /locus_tag="ACL73L_000148"
     CDS             54344..54664
                     /locus_tag="ACL73L_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HGS0771202.1"
                     /translation="MEIIVPDQIIMGLILYAGDAKQHIYKALDYIKNGTCERCEEEIQ
                     LADAALLEAHNLQTKFLAQEASGTKTEITALFVHSQDHLMTSMTEINLIKEIISLRKE
                     LHKK"
     gene            54710..55018
                     /locus_tag="ACL73L_000149"
     CDS             54710..55018
                     /locus_tag="ACL73L_000149"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGS0771203.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQSSGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
     gene            55035..56333
                     /locus_tag="ACL73L_000150"
     CDS             55035..56333
                     /locus_tag="ACL73L_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HGS0771204.1"
                     /translation="MSKMDVQKIIAPMMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFEAMIPAFVIFLSSMIVYILAKSLTNGGTFIEMIYSAIQV
                     PLQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASGNLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFAAKSKQYQALGKVAAFPAIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAVIVYGAIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVVTQLLVLVISTVVYFPFFKIQDRLAYQNEIKQS"
     gene            56484..58922
                     /locus_tag="ACL73L_000151"
     CDS             56484..58922
                     /locus_tag="ACL73L_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HGS0771205.1"
                     /translation="MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVL
                     ATRAYERYKEQPNVLKRAYMLKEILENMSIYIEEESMIAGNQASSNKDAPIFPEYTLE
                     FVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKVALDLTDPASIDKYHFYDSI
                     FIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPATTFAEAIQSV
                     WFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITIN
                     KVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRY
                     HAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAV
                     PGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGHFKDMKNFSELENAWDK
                     TLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQV
                     GIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDD
                     DYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDG
                     RNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTLAKEEDK
                     LKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSK
                     ATQDDIIGRTEHTL"
     gene            58941..59609
                     /locus_tag="ACL73L_000152"
     CDS             58941..59609
                     /locus_tag="ACL73L_000152"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HGS0771206.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAHEIRRQAGDDIFIKVPVTPAGLRAIKVLK
                     KEGYHITATAIYTVIQGLLAIEVGADYLAPYYNRMENLNIDSNSVIRQLALAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGADVFESAFAMPSIQKAVDDFSDDWFVIQN
                     SRSI"
     gene            59627..60715
                     /locus_tag="ACL73L_000153"
     CDS             59627..60715
                     /locus_tag="ACL73L_000153"
                     /EC_number="1.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008888 - glycerol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HGS0771207.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNCPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
     gene            61170..63671
                     /gene="leuS"
                     /locus_tag="ACL73L_000154"
     CDS             61170..63671
                     /gene="leuS"
                     /locus_tag="ACL73L_000154"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HGS0771208.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKD
                     MQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFTVLAPEHELVDAITSSEQ
                     AEAVADYKHQASLKSDLARTDLAKEKTGVWTGAYAINPVNGKEMPIWIADYVLASYGT
                     GAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGNVEEAAYTEDGLHVNSDFLDGLNKEDA
                     IAKIVAWLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPETELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLDVVPTKEPFQKLFN
                     QGMILGTSYRDHRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITSKEILAENNGALD
                     KVYNETVKAVTEQIESLKFNTAIAQLMVFVNAANKEDKLYVDYAKGFIQLIAPFAPHL
                     AEELWQTVAETGESISYVAWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDLSREEL
                     QEIALADEKVKAEIDGKEIVKVIAVPNKLVNIVVK"
     gene            63810..>64279
                     /locus_tag="ACL73L_000155"
     CDS             63810..>64279
                     /locus_tag="ACL73L_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771209.1"
                     /translation="MNRLVNDSRKCEILLFKISIIVVSVIVLVMSIVGAYYFKNCLWD
                     KESLNLTLTLDTLFAVFITFFSFSNDKSSNIFGISPNEIIRTQSEVYQFLFTKSFATL
                     LIVTNLLSIIHYFGAKNLIVSFNETYPRIQSSVGIMWLPLFGILIIYFIWLLIEG"
BASE COUNT        19689 a        11632 c        13795 g        19163 t
ORIGIN      
        1 tatcaattta tccaaaggaa aaaatttcat atttctatgg catttctcaa acttcctatt
       61 ttataggtga tactctggga ggatttattg gaggttggct atttaaccaa gatatgaaat
      121 acagtattta tctgttttct gtgttattat tattcaacgc tgtgttattt aacagatttt
      181 acttacggaa taataaaaat tcttaaggga cctctaaaaa aaacagaaga ttaaaataca
      241 gtaaaaaaat caactaaaat agtcccaaag taaagaaata cttgaaccta ttttagctgg
      301 ttttcttttt tttcacctct aattgaggat tattctattt tagggtatac tcccccatgt
      361 tttcaaccct aatcgttgga tttgttcgaa acgacagata ttatacaaca aatttgtcaa
      421 aatcacattc gtttttgtac gcgctaatcc aattcctcta aaatgacttc ctttcatcgt
      481 atttttaata acaccaaaaa catgttccac acgacagcgg atacgagaaa tagactgatt
      541 gatacgctta tccaaatcag tcaaggtttt atttcgaaaa gcactatcat caaagatcgg
      601 ctccttactc tcacacaact cttccaagac gcgagaatcg tgaatattag cagaagtaat
      661 ctcaaatttc ttgattagtt ttgatttttg gtcgatacta atactcaatg aaaatcaaag
      721 agcaaactag gaagctagac gcaagttgct caaaacactg ttttgaggtt gcagatagag
      781 ctgacgtggt tttaatttga ttttcgaaga gtataatatg atttgtataa ccaaagtaag
      841 ttggttctcc ctagtccatc ctgtatccct atctttttga gaaggcttcg ctgtattcca
      901 atctgtcgga atctttcctt ttttgatttg ttgattctct tctcgtgtat tgcgttgttt
      961 aggggattct acaaatgggg catctactat ctgtccctga tgagctaata agcctttttc
     1021 tgctagtgtt agataaaatt actcagataa ttcttgttct ttctctgatt tggtcaagcg
     1081 ttctctatac aaccagatgg tttttgcatc atgtacttta cattcatcac aacctgtgga
     1141 atgacgaaat gacagacgat gaaagagttg atattccatc gcatcatctg ataaattatg
     1201 gagatgttgg atcaagagca ccttaaatat catgaaaaaa tcatgcggag ggagtccgcc
     1261 ttttgatggt ttctaaaaat ctgctcaaga ataggtctaa tttgctccca atccattgcc
     1321 ttatctaagc cttccaatgg attgcccatt gaactcaatt ttgatagtgt ttgttcatca
     1381 tctataaaaa attccatcgc ctaacctagc ttacctctat ctctagtatc tcatgtttta
     1441 cgattttttt gggtttttag aggtgccctt aaattcgttc attaatgtta cgagactggc
     1501 cgaaaattag tctctaaata aaaccacaca gtgatttcag tcttgaatcc aatcaaactg
     1561 aaagaagcac tatgtggttt ttgaatagtg ggctagattc ttgatcaatt tggtgagatt
     1621 catggtaatc gcccaataac gaggtatatt gaaaaatcta cagactagag aactcactga
     1681 tagttcctaa tctggagatt tcttatttgc acttttcttg tacaacttta gtccatggta
     1741 aataagcctc taaaacatct ttatttacga gagtctcctc gcctagcttt ctagtttgtt
     1801 ctttaatttt tattgagtat accactattt tactccctct ggcaagggac tttgtctatg
     1861 tggagggatt gggctcctat gtggtggagc ttttctgttc tttctgaaat atggtataat
     1921 agcactaatc aatttctagg aaaatagata cagaaagggg ctgaaagatg tctcatatta
     1981 ttgaattgcc agagatgctg gcaaaccaaa tcgcggctgg agaggtcatt gaacgtcctg
     2041 ccagtgtggt caaagagttg gtagaaaatg ccattgacgc gggctctagt cagattatca
     2101 ttgagattga ggaagctggt ctcaagaagg ttcaaatcac agataacggt tatggaattt
     2161 cccacgatga ggtggagttg gccctgcgtc gccatgcgac cagtaagata aaaaatcaag
     2221 cagatctctt tcggattcgg acgcttggtt ttcgtggtga agccttgcct tctattgcgt
     2281 ctgttagtgt cttgactctg ttaacggcgg tggatggtgc tagtcatgga accaagttag
     2341 tcgcgcgtgg gggtgaagtt gaggaagtca tcccagcgac tagtcctgtg ggaaccaagg
     2401 tttgtgtgga ggatctcttt ttcaacacgc ctgcccgtct caagtatatg aagagccagc
     2461 aagcggagtt gtctcatatc attgatattg tcaaccgtct gggcttggcc catcctgaga
     2521 tttctttcag cttgattagt gatggtaagg aaatgacgcg gacagctggg actggtcaac
     2581 tgcgccaagc tattgcaggg atttacggtt tggtcagtgc caagaagatg attgaaattg
     2641 aaaattctga cctagatttc gaaatttcag gttttgtgtc cttgcctgag ttgactcggg
     2701 ctaaccgcaa ttatatcagc cttttcatca atggtcgtta tatcaagaat ttcctactca
     2761 atcgtgctat tttagatggt tacggaagta agctcatggt aggccgtttt ccactggcag
     2821 tcattcacat ccatatcgac ccctatctag cggatgtcaa tgtgcatcca accaagcaag
     2881 aggtgcgaat ttctaaggaa aaagaactga tgacgctggt ctcagaagct attgccaaca
     2941 gtctcaagga gcaaaccttg ataccagatg ccttggaaaa tcttgccaaa tcgaccgtgc
     3001 gcaatcgtga gaaggtggag cagaccattc tcccactcaa agaaaatacg ctctactatg
     3061 agaaaactga gccgtcaaga cctagtcaaa ctgaagtagc tgattatcag gtagaattga
     3121 ctgatgaagg gcaggatttg accctgtttg ccaaggaaac cttggaccga ttgaccaagc
     3181 cagcaaaact gcattttgca gagagaaagc ctgctaacta cgaccagcta gaccatccag
     3241 agttagatct tgctagcatc gataaggctt atgacaaact ggagcgagaa gaagcatcca
     3301 gcttcccaga gttggagttt ttcggacaaa tgcacgggac ttatctcttt gcccaagggc
     3361 gagatggact ttacatcata gatcagcacg ctgctcagga acgggtcaag tacgaggagt
     3421 accgtgaaag cattggcaat gttgaccaaa gccagcagca actcctagtg ccctatatct
     3481 ttgaatttcc tgcggatgat gccctgcgtc tcaaggaaag aatgcctctc ttagaggaag
     3541 tgggcgtctt tctagcagag tacggagaaa atcaatttat tctacgtgaa catcctattt
     3601 ggatggcaga agaagagatt gaatcaggca tctatgagat gtgcgacatg ctccttttga
     3661 ccaaggaagt ttctatcaag aaataccgag cagagctggc tatcatgatg tcttgcaagc
     3721 gatctatcaa ggccaatcat cgtattgatg atcattcagc tagacaactc ctctatcagc
     3781 tttctcaatg tgacaatccc tataactgtc ctcacggacg tcctgttttg gtgcatttta
     3841 ccaagtcgga tatggaaaag atgttccgac gtattcagga aaatcacacc agtctccgtg
     3901 agttggggaa atattaaaag tataaaaaag tctgggaaaa attttcaaaa tcaaaaaaac
     3961 gcataaaatc aggtgttcaa aaaccttgat tttatgcgtt ttatcatgga aatagttact
     4021 tcattttttc ctaattcttt tcgaaactct ttttaaacga cgtcagtttt atcagtaatc
     4081 tcaaaacagt gttttgagct aattttgcca gttttgtctg taacatcgaa gttgtgtttg
     4141 accactctgc gactggtttc ctagtttgct ctatgatttt cacagagtat taaattgcga
     4201 ttttgccaag tttctttatt cgtctaaaag tagagtctgt tctatgcgtc taatgtacga
     4261 attaggttga ccatttcaat agctccttgt gcacactcag aacccttatt tcctgcttta
     4321 gtaccagctc gttctatggc ttgttcaatt gtatctgtcg ttagcacacc aaacataaca
     4381 ggaatttcgc tatttaaact gatttgggcg attcccttag atacctcgct acatacataa
     4441 tcataatgac ttgtatttcc tctaatgaca gctcccaagc agataattgc atcatatttt
     4501 ttactttttg ccatttttga tgcaatcagt ggtatttcaa aagctcctgg aacccaggct
     4561 acctcgatat ctttctcgtt tacattctct cttttgagat tatctagtgc tccagataat
     4621 aattttgaag ttataaattc attaaatctc gctacaacaa tacctatttt aatattgttt
     4681 gctactaaat taccttcata agtgttcatt tatttttcct ccatatttaa aatgtgaccc
     4741 attcgatttt tctttgtttc taaataaaaa ctatcgtaag gattggcttc tatttcgatt
     4801 gatattctac tggaaatggt aattccatat ttttctaact gttcaacctt gtcaggatta
     4861 tttgtcagta aatgaagtga ctgaagtccc agatctttaa gcatttgtgc tccaatatga
     4921 tattctctta aatcaccttc aaagcctaat gcaagattgg catcaagcgt atccatgcct
     4981 tgatcttgta aatgataggc ttttaattta ttgataagtc caattcctcg tccctcctgt
     5041 cgcaagtaaa gtaagacacc cgaaccattc tcaacaatca ttttcatagc tttatcgaat
     5101 tgctgtccac aatcgcaacg taaagagcct aaaacatctc ctgttaaaca ttcggagtgg
     5161 acccgacata atacattggc ttcatcctct atgtttccca taataagagc aagatgatgt
     5221 tccccattta gtttatctat atagctaatt gctttgaaat taccgtatct agtaggcata
     5281 ttgacagttg aaactcgttc taccagctga tcatatactt ttctatattc ttgtaattct
     5341 ttgatggtaa ttagtggaat gttgtgtttc ttcgaaaact gaattaaatc atctgttctc
     5401 atcattttgc catcatgatt cattatttca caacataggc cacactcttt tagtccagct
     5461 aattttaata aatcaacagt tgcttctgtg tgtccatttc tttctagaac accacctttt
     5521 tttgcaatta aaggaaacat gtgtcctggc ctgcgaaaat cagagggtgt tatatcttca
     5581 gctacacaca tacgtgcggt cagtcctctt tcctcggcag aaatacctgt ggtcgtttct
     5641 ttataatcaa ttgaaactgt aaaagcagtc ttatgattat ctgtattgtt ttcaaccata
     5701 ggtgaaagca ttaattgatt agctaaactt tcgctcatag gcatacaaat taatcctttg
     5761 gcataagtag ccataaaatt aacattttct gttgtagctg cttgtgcaga acaaattaag
     5821 tctccttcat tttctctatc cttgtcgtct ataacaagaa caagtcgtcc cttctgcaat
     5881 gcttctaatg cttcttgtat ttttcgatat tccattgact gattatcctt tctgctaaaa
     5941 tccattttga tataatagtt ccttagatat ttctgatttt ggagagttat ccatcagttt
     6001 ttgcacatat ttacctaaga tatcattttc aagatttact gtactcccga cttgtttact
     6061 cttaagaatg gtttgttcca aggtatgagg gataacagat actgaaaagt ttactttgga
     6121 gactttagcg acagtcagac taatgccgtc aattgtaata gatccttttt caactattaa
     6181 atctaaaatt tctttttgtg tgttgatttg ataccataca gcattatcat ctttttttat
     6241 tgacgagatt tttcctgtac catcaatgtg tcctgtaacg acgtgacccc caagtcgacc
     6301 gttgacagat aaggctcttt ctagattcac ctcacttcca tgttttaata gagtaagagc
     6361 tgttcgactc catgtttcat tcattacatc aactgtaaag gattgatggt tgaaatgagt
     6421 aactgtaaga cagataccat ttactgctat actatcgcct aaatggatat ccgttaatat
     6481 ttttgaggct ttaattgata gtttacaatt acgagagtct ttctgtattc tttcaacttt
     6541 tccgatttct tcaattattc ctgtgaacat ggataaatca cttcactttc tatgagatag
     6601 tcatttccta tttgagaaaa agcataaggt ttcaatctaa tagcgtcatt tggcaaagaa
     6661 atgccttcac ctccgacagg aaacttggca ctgcctccaa aaatttttgg tgcaatatat
     6721 attttcagct catcaacaat ttgttgttcc aaagcactcc aattcattag actgccccct
     6781 tctagaacta ggctatcaat ctgcatgttt cctagatgtt gcattaaact cgataagtct
     6841 atatgattgc cttttttctt tatggaaagt atttcacagc catgattttg atatagcttc
     6901 attttatttt tgtcttcaga ggaagtggca atgtaagttt taatatcatt tgctgttttt
     6961 acgattttag aggtaagagg agttcgtaaa tgtgtatcgc atatgatacg gataggattt
     7021 ttcccttcct ccaatctaca tgtcagcaaa ggatcgtctt gaataacagt attgactccc
     7081 accataattg cactaacata gtgtcgtaac tgatgcacat gctttcttgc ttcttcttca
     7141 gtaatccatt tggattgatt tgttttagtg gctatttttc catccattga cattgcatat
     7201 ttcataaaaa catagggtac atgctgggta atatactttc taaaactttt tattaagtta
     7261 agacactcat tttctaaaat tccaacagta acttgaagat tattttcctc aagtatcttt
     7321 actccttttc cagatacaat aggattacag tctaggcttc caatgactac tcttgtaata
     7381 ccactatcga ttatagcatc tatacaggga ggtgttttcc cgaagtgaca acagggttca
     7441 agtgttacat aaagcgtcgc tccgacaggg gattctctac agtttttaag agcatttctc
     7501 tcagcatgtg ggccaccaaa aaactcatga taaccttgtc cgatgatgtg attatctttt
     7561 acaataactg cgccgaccat aggattggga ttgacgtaac cagccccttt ttgtgccagt
     7621 tttattgcta atttcatata ttttgaatcg ctcatctcac tacctccaaa aaaaatatac
     7681 cttgaatagg ggactactca aggcatacaa aagaaaactt atgcgattaa caaaaatact
     7741 ctgaaatgac aagtaatcat ttcagagcac gcaaaaagca caaatatact tttatcttct
     7801 ttcatccaga ctatactgtc ggctttggaa tttcaccaaa tcatgccttt cggctcgtgg
     7861 gctataccac cggtagggaa ttacaccctg ccctgaagat agttattcaa ttacagatga
     7921 ttatagtact taattttgaa tatgtcaaca gataaatacc gattgttttt gatatactgt
     7981 atttgtgata atcgattctc gctcctcgga taaagaaaat atgatatact agataaacga
     8041 aataagagag aaggaatact atgtacgcat atttaaaagg aatcattacc aaaattactg
     8101 ccaaatacat tgttcttgaa accaatggta ttggttatat cctgcatgtg gccaatcctt
     8161 atgcctattc aggtcaggtt aatcaggagg ctcagattta tgtgcatcag gttgtgcgtg
     8221 aggacgccca tttgctttat ggatttcgct cagaggatga gaaaaagctc tttcttagtc
     8281 tgatttcggt ctctgggatt ggtcctgtat cagctcttgc tattatcgct gctgatgaca
     8341 atgctggctt ggttcaagcc attgaaacca agaacatcac ctacttgacc aagttcccta
     8401 aaattggcaa gaaaacagcc cagcagatgg tgctggactt ggaaggcaag gtagtagttg
     8461 caggagatgg ccttcctgcc aaggtcgcag tgcaagcaag tgctgaaaac caagaattgg
     8521 aagaagctat ggaagccatg ttggctctgg gctacaaggc aacagagctc aagaaaatca
     8581 agaaattctt tgaaggaacg acagatacag ctgagaacta tatcaagtcg gcccttaaaa
     8641 tgttggtcaa ataggagcag agaatgacaa aacgttgttc gtgggtcaag atgaccaacc
     8701 cgctctacat cgcctatcat gatgaggagt ggggtcagcc cctccatgat gaccaagcat
     8761 tgtttgagtt gttgtgtatg gaaacctatc aggcaggcct gtcttgggaa acggtactca
     8821 acaaacgcca agctttccga gaagcctttc atagctatca aattcactca gtcgcagaga
     8881 tgactgagac tgaattggaa gccatgctgg agaatccagc tatcattcga aatagagcca
     8941 agctttttgc tacacgcgct aacgcccaag cctttctaca gttacaggca gagtacggct
     9001 cttttgatgc ctatctttgg tcttttgttg aggggaaaac tgtcgttaac gatgttcctg
     9061 attatcgcca agccccagct aaaacaccct tatctgagaa attagccaaa gatctcaaaa
     9121 aacgaggctt caagttcaca ggcccagtcg ccgtattgtc ttttctacag gctgcagggc
     9181 tagttgatga ccacgagaat gattgtgagt ggagaggtct taaatgatgt ctaacaaaaa
     9241 taagggaatt ctgatttttg cgattctcta tacagtcctc tttatgtttg atggcgttaa
     9301 attgctggct tctttaatgc catctgccat tgcaaattat cttgtttatg tagttttagc
     9361 tctatatggc tccttcttgt tcaaggatag attgatccaa caatggaagg agattagaaa
     9421 gactaaaaga aaattcttct ttggcgtctt aacaggatgg ctctttctca ttctgatgac
     9481 tgttgtcttt gaatttgtat cagagatgtt gaagcagttt gtgggactag atggacaagg
     9541 tctaaatcag tctaatattc aaagtacctt tcaagaacaa ccactactga tagctgtttt
     9601 tgcttgtgtc attggacctc tggtagaaga attatttttc cgtcaggtct tattgcatta
     9661 cttgcaggaa cggttgccag gtttactaag cattattctg gtaggacttg tttttgcttt
     9721 gactcatatg cacagtttgg ctctatcaga gtggattggt gcagttggtt acttaggtgg
     9781 aggccttgcc ttttctatta tttatgtgaa agaaaaagag aatatctact atcccctact
     9841 tgttcacatg ttaagcaaca gcctctcctt aatcatttta gctatcagta tagtaaaatg
     9901 aaataagaac aggacaaatc gatcaggaca gtcaaatcga tttctaacaa tgttttagaa
     9961 gcagaggtgt actattctag tttcaattta ctgtaatatg tgatgaaaat gccagtaatg
    10021 ataccgagaa aaaagctgag aaacttttcc cagctttatt tgttatagtc aaagcgaatg
    10081 acttgttcct gtgcatctac atgagcatgg accccaaagg gtacaattgc tcttggagtt
    10141 gcgtggccga cattcagatt atagacaatc gggatattgc tgtcaatgat atccaatagt
    10201 gcctctttat agtcgtcatg gaaagtttca tccataggtt ttccgaccaa gagtccattg
    10261 atgaccgcga atatgccagt gtcctttaaa gttagcaaca tctttttgaa gtcttctggc
    10321 ttaggctttt cttcgcttgt ttcgagcaag aggattttcc cttcccagtc tgacaagtca
    10381 gggaaaagtt tgtatttttg gcagagctcc gtgctatctg cgtatcgaga gttgtcaaag
    10441 atatcgtaga gggattcgag gcaaccaccg aggattttcc cctcgaattg ggcccttcct
    10501 tgcaacaagt caaaacctgt atttgtatga ctgacacgag gtgttcccag ggccgtggga
    10561 ctaaaatcag ttcgttcctc ataccaaacg tcactagggc ggatttctga aattcttccc
    10621 gtctcaatca attctttaaa gtagtgaagg ctataggcta gcatttcttt gtctaattca
    10681 caaatgtctg ctaaaaagga ttgaccataa aaagtcttga ttcctaattt atgcaacatg
    10741 aggtggttca tggttgtatc cgagaagcca agaaaaattt tttgtttgat aaccttttgg
    10801 agttggtcat tttcaaaaag ataaggtagc aagcgatagg tatcgtctcc accgatggca
    10861 cataggatca tgtcgatgct atcatcagaa aaggcatgaa tcaaatcctc tgcacgagct
    10921 tcaggatggt ccttgataaa gtctaatcct tttaacgaat ggggcaaaaa gatgggattg
    10981 agtcccagat ccttgagacg ttggataccc aagtccactt cgtgtttgac aaaatcctct
    11041 ccgataatgc cactagataa actaacaata ccaatagtag aaaccatatc tcatcctcct
    11101 agaaatagat tgagcctatt ttatcacaaa atataagaga aagctatgtg ggatttcaga
    11161 agaatacttt taatactctt cgaaaatctc ttcaaatcac gtcagtttta tttgcaacct
    11221 caaagctgtg ctttgagcaa cctgcagcta gcttcctagt ttgctctttg attttcattg
    11281 agtataactc aaaaaagtaa gtgaaaaagc aaccgcacct gcagttgctt ttatcgtttt
    11341 cttattcttg atagcgtgtt ggtcctgcac ttgcactacg gatattcaag cgtttcttga
    11401 gatagaagcg aagggctagg agggctgctc caataatagc tagtggcaat ggatccagta
    11461 ctgggttaag gctagctggt aggaagcttg ttgcaaagaa gacaagcaac caaaggaaca
    11521 tagaagctag gataactagt acagatttcc agaaaggtgg acgttgactg cgatccatat
    11581 ctggtccata gtattggtaa acaaagtagt acatcaagta gaaggcaaat ccaccaacca
    11641 gtccaactaa tagaagagta atcaatccat agccgaaagc ttgatctgcc gcaaagaagg
    11701 ttgtgagggc gctgacaagg gcaaagaggc tagtgatgaa aagagctgag tccataatca
    11761 tcagttttgg gtcatcattt tcttttggat gctctttttc gtactgctct ttgacagtga
    11821 agctatgagc ccaatgagtt ggtgcgccgt atagggaacg ggcagttgta cctttagatt
    11881 gctcctcaag gatttgggga atgacttcct caaaaatagt ctggatttca gcgtctgttt
    11941 tcccatcttt gatgaattgt tgggtagcaa tgtggacaaa ctcttggttt ttcttagtta
    12001 atttttgtag atcaatttga gacataggaa ctcctcttag aaccattttt tatggatgag
    12061 atagagagtg agcgagacac tcatagcaaa ggcgataaag acgattaacc agaaggcatt
    12121 tggctctccg tttaggggaa tttcattatc cttaaagttc atcccgtagg cagaaaagac
    12181 catggttggg atggacatga cgatggtcac aagggccaag gttttcatga tgttgttctg
    12241 gttgttagaa atgatagagg caaaggtctc tgtcatagaa tgcaagacgt ttccataaat
    12301 atctgccatc tcgatggcct gttgggtttc aatcagggtg tcttcaagca ggtcttcgtc
    12361 ctcaaggtat ttcttgatat tgctggttga actggtcaat ttcttaatca cgcgctcatt
    12421 tgttttgagg gaggccttga aatagacgat agttttttcc aattccatga gctcaatcaa
    12481 ttcttcatta cgagttgatt gatgcagttg actttcgatt tgttcactct tgcggtcgat
    12541 tgaacgaagg gctgttaggt aaagctctgc attgcgataa agaatttgaa agataaaacg
    12601 tgaacgcatg aaggtataga aattacgcaa tcgacggttg ataaagacat caaggacagg
    12661 tagtggttcc aaacacgtag tgataatggt ttcctcagtg atgataatac caagcgggat
    12721 ggttacgtag taggtgcggt tatttctttc ctccgtgacc ggcacgtcta cgataatcag
    12781 ggtatactcg tcttcaatgg taatacgaga catttcttcc gcatcgagcg gtgctcgaag
    12841 gtcggcaata tcaatatcga aggtgttggc gatttcgagt gattcatttt gagtcggatt
    12901 gacgagattg atccaagtac ccggttcaag cgtatcgatc tctttaaatt cagttgttgt
    12961 agagagaaaa acttgtttca tatcccttat cctttctcat ttttcagatt ttttcacact
    13021 gtactattat actacaaaat cggccttttg ggtaatagaa tctcactttt tttggtataa
    13081 tggtaagcaa taatggacta gaaagaacaa agatgcaaga taaaattgtc attcatgggg
    13141 cgcgtgccca taatttaaaa aatattgatg tggagattcc gcgagacaag ttggttgtcg
    13201 tgactggctt gtcaggttca gggaagtcca gtctggcttt tgatactctc tatgcggagg
    13261 gacaacgtcg ctatgtagag agtttgtctg cctacgctcg tcagttcttg ggaaatatgg
    13321 aaaaaccaga tgtagatgcc attgatggtc tcagcccagc tatttccatc gaccagaaaa
    13381 cgactagtaa aaatcctcgc tcgacggtgg gaacgacgac tgaaatcaat gactatctgc
    13441 gtctcctcta cgcacgtgtg gggacgcctt actgtatcaa cggacatgga gctatcaatg
    13501 cttcttctgt ggagcaaatc gttgataagg ttttggagtt acctgaacgc cagcgcttgc
    13561 agatcttggc tcctgtcatc cgcaagaaaa aaggccaaca taagagtgtt atcgagaagg
    13621 ttcagaaaga cgggtatgtt cgtgttcgtg tggatgggga agtctatgat gtgaccgaag
    13681 tgccagagtt gtctaagagc aagcaacaca atatcgatgt cgtggttgac cgtattgtta
    13741 tcaaggaggg cattcgtagc cgtctctttg attccattga agctgccctt cgtatcgcag
    13801 aaggttatgt cattatcgac accatggacg actcggagtt gctcttctct gagcattatg
    13861 cctgtccagt ttgtggattt actgtcccag agttagaacc tcgtctcttc tctttcaatg
    13921 ctccctttgg ctcttgtagt gagtgtgacg gcttggggat taagctggag gtggatactg
    13981 atttggtagt gccagatgcc agcaaaacct tacatgaggg agcgctggca ccttggaatc
    14041 ctatctcatc caactactat ccaaacatgt tagagcaggc catgaaagtc tttggagtgg
    14101 ctatggataa gccttttgag gacctgtcag aagaagataa gaacttgatt ctctacggct
    14161 cagatggtaa ggaattccat ttccattatg aaaatgaatt tggtggtgtg cgcgatatcg
    14221 acattccgtt tgagggagtt atcaataata tcaaacgtcg ttaccatgaa accaatagcg
    14281 attacactcg cactcagatg cgtctctaca tgaatgagct gacctgcgga acctgtcaag
    14341 gctatcgtct caatgaccag gccttgtctg tccgtgtggg cggccagcaa gggccacata
    14401 ttggagaaat ctcagacctg tctatcgctg accacttgga cttggtgagc cagttaactt
    14461 tgtctgaaaa tgaagccatc attgctcgtc ccattctcaa ggaaatcaag gatcgtttga
    14521 ccttccttaa taatgtgggt cttaactatc taaccctgtc acgttcagca ggaacccttt
    14581 caggtgggga aagtcagcgc attcgtttgg caacccagat tggttccaac ctatcaggtg
    14641 tcctctatat cctagacgag ccgtcaatcg gtcttcacca gagggacaat gaccgcctga
    14701 ttgccagtct gaaaaagatg cgtgacttgg gcaatactct tatcgtggtg gaacacgacg
    14761 aagataccat gcgtgaagct gattatctga ttgacgttgg tcctggtgcc ggtgtttttg
    14821 gtggtgagat tgttgcagca ggtacaccta agcaagtggc tcgcaacagc aagtctatca
    14881 caggtcagta cttgtcaggc aaacgtgtca tcccagtacc agaagagcgc cgtgtcggaa
    14941 atggtcgttt tattgaagtg acaggagcgc gtgagaacaa cttgcaaaat gtcacggctc
    15001 gctttccact aggaaaattt atcgcagtga caggtgtgtc gggttcaggg aaatcgactc
    15061 taatcaacag cattctcaaa aaagccattg cccagaagct caaccgtaat tcagacaaac
    15121 ctggtaagtt taagactatt acagggattg agcatgtcga ccgtttgatt gatattaacc
    15181 agagccctat cggacggacg ccgaggtcta acccagctac ctatacagga gtttttgacg
    15241 atatacgtga cctttttgct cagacaaatg aagccaagat tcgaggctac aagaaggggc
    15301 gcttcagttt caacgtcaag ggaggtcgct gtgaagcttg ctcaggtgac gggattatca
    15361 agattgagat gcacttcttg ccagatgttt atgtggcttg tgaagtttgc cacggaaccc
    15421 gctataacag tgaaacccta gaagttcact acaaggaaaa gaatatttcg caggtcttgg
    15481 atatgacggt caacgatgcg gtagaatttt tccaacacat tccgaaaatt caacgcaaac
    15541 tccagaccat caaggatgta ggtctaggct atgtgacctt gggacagcca gctaccaccc
    15601 tttctggggg agaagcccaa cgtatgaagt tagctagcga actccacaaa cgctcgacag
    15661 gaaaatcttt ctacattctg gatgagccga cgacagggct tcatacagag gacattgctc
    15721 gcctgctcaa ggttttagct cgctttgtag acgccggcaa tacagtcctc gtcatcgagc
    15781 acaatctgga tgttatcaag acggcagacc atatcattga cttgggacct gagggcggtg
    15841 tcggtggtgg aaccatcatc gtaacaggaa ctccagaaga agtagctgcc aacgaagcca
    15901 gctatacagg acactatttg aaaggaaagt tacatcatga ataaacgcgt acaagcattt
    15961 ctagctaaaa tgcaagaaaa agaactagat ggtatcatca tcaacaatct taaaaacgtc
    16021 tattatttga ctggtttttg gggctcaaac ggaacagtct ttatcagccg tgaccgtcag
    16081 gtcttagtga cagactctcg ctatatcatc gcagctaagc aagaaaccag tggttttgag
    16141 attgtggctg atcgtgatga attggctgtc attgcaggaa ttgttaagga catgaacttg
    16201 actcgtatcg gttttgaaga tgagatttca gtgtcttatt accaccgtat gcaggcagct
    16261 tttgcaggtt tgaacttgct tccacaaact cagtttgtgg aaggtcttcg tatgattaag
    16321 gatgaggcag agattgcagc gattcgcaag gcttgttcta tctcagacca agctttccgc
    16381 gatgcgcttg actttatcaa accaggaaaa actgaaattg agattgccaa cttccttgat
    16441 ttccgcatgc gtgagttggg agcatctggc ttatcttttg atacgatcct agctagcggt
    16501 atcaattctt ctaaacccca tgcccatcca atgcacaaac cagtggagtt gggagaagcc
    16561 atcaccatgg acttcggctg tctctatgac cactatgtca gtgatatgac ccggactatc
    16621 tatctagggc atgttagcga tgagcaggca gagatttaca atacggttct aaaagctaac
    16681 caagccttga ttgaccaagc taaggtaggc ttaggtttcc gtgactttga caaaatccct
    16741 cgtgatatta tcattgaggc aggttatggt gactacttta ctcacggcat tggccacggt
    16801 attggtctgg atatccatga ggaaccctac tttagtcaga cttctacaga aactattaag
    16861 acaggtatgg ccttgaccga tgaaccaggt atctatatcg aaggcaaata tggcgttcgt
    16921 atcgaggatg atatcctgat tacagagaca ggttgtgaat tattgaccct agctccaaaa
    16981 gagttgatag tcatttagtt gtttgtcaag aaaaaagtac gaaaatgacg aaaaaagtca
    17041 aaaaaaatta aaaataggtc gcaagtcgga tgttttttat ggtataatag actaaactga
    17101 tagtaacatg tagcgaaagg ggtaggtaca tgattaaaat ttatacagtc tcaagttgta
    17161 ctagctgtaa aaaagcaaaa acctggctca atgcccacca gttaagttat aaagaacaaa
    17221 accttggtaa agaaggaatt acgagagaag aattactgga tattctaacc aaaacagata
    17281 acggaatagc cagcatcgtt tcgtctaaaa atcgctatgc caaagccctt ggagtggata
    17341 ttgaagattt gagtgtcaat gaagttctca atctgattat ggaaacaccg agaattttaa
    17401 agagcccaat ccttgtagat gaaaaacgcc tgcaagttgg ctacaaggaa gacgatattc
    17461 gtgccttcct accacgctct gtccgtaatg tagaaaatgc agaagcacgt ttgcgtgcag
    17521 ctctataaac atcaaggctg ggagcacttc ccagtcttat tctattttaa tttcaaaaag
    17581 aaagaagaaa gaaatgaaaa aaatagttct tgttagtcta gctttccttt ttgtcctggt
    17641 tggttgcgga cagaaaaaag aaactggacc agctacaaaa acagaaaaag atacgcttca
    17701 gtcggcattg ccagttattg aaaatgctga gaagaataca gttgtaacta agactttggt
    17761 cttgcccaag tcagatgatg gtagccagca gacacaaaca attacttaca aagacaagac
    17821 ttttttgagt ctaactatcc aacaaaaacg tccagtctct gatgagttga agacttatat
    17881 tgaccaacat ggagtggagg aaactcaaaa agctcttctt gaagcggagg agaaggataa
    17941 gtctatcatt gaagctcgta aactggcagg tttcaaactt gaaacaaaac tattgagcgc
    18001 aacggaactt caaacaacga ctagttttga ttttcaagtt ctggatgtca agaaggcttc
    18061 ccagttggaa catctgaaga atattggttt ggaaaatctt ttgaaaaatg aaccaagcaa
    18121 atatatttca gatagattgg caaatggcgc gacagaacaa tagaaaatcc aaataaatag
    18181 actgcctgaa ttgtagaagg taaggaatta tgctataatg gtactattct aaggaaagag
    18241 ggtgttagaa tgggatttac tgaagaaaca gtacgtttta aattggacga ttccaataaa
    18301 aaagaaatta gcgaaacttt gacagatgtt tatgcttcgt tgaacgataa gggttacaac
    18361 ccaattaacc aaatcgtagg ttacgtattg agtggagacc ctgcctacgt tcctcgttat
    18421 aataatgcac gaaatcaaat ccgtaagtat gagcgtgatg aaatcgttga ggaattggtt
    18481 cgctactacc tcaaagggca aggagtcgat ctataaccta tgagaattat gggattggac
    18541 gtcggttcaa aaacggtagg ggtggcgatt agcgatccgc ttggttttac agctcaaggg
    18601 cttgaaatca tccagataaa tgaagaacaa ggcaaatttg gttttgaccg cgttaaggaa
    18661 ttggttgata cttacaaggt ggaacgattt gtagtgggct tgcctaaaaa catgaacaat
    18721 acaagtggac cgcgcgtaga agctagtcaa gcctacggag caaagctaga agagtttttt
    18781 ggtttaccag tagactatca ggatgaacgc ttgacaacag tggctgctga gcgcatgttg
    18841 attgaacaag cagatatcag tcgcaataag cgcaagaaag tcattgataa gttagcagct
    18901 cagctgattt tacaaaatta tttagataga aaattttaat ataaaggaga ggctatgtca
    18961 cacgatcata accacgacca cgaagaacgt gaactaatca cactagtaga tgagcaagga
    19021 aatgaaacct tgtttgaaat ccttttgaca attgatggaa aagaagaatt tggtaaaaac
    19081 tatgttcttc tagtaccagt taacgcagaa gaagacgaag acggacaagt tgaaatccaa
    19141 gcttactcat tcattgaaaa tgaagatgga acagaaggcg aattgcaacc aatcccagaa
    19201 gactcagaag acgaatggaa catgattgaa gaagtcttca acagctttat ggaggagtga
    19261 tacagtctgg gagactgtat cagcccaact cccagaaatg gggaagagtt ggaacgtcca
    19321 gtggatgttt ttagcccagt tcctttaaat aagagagaac tggtaggttc ggggggacgt
    19381 ttttagccct gcgctagaaa ttagaaacgc aggtagtctg agaaactgta tcagcccaac
    19441 tcccagaaat ggggaagggt tggaacgtcc agtggatgtt ttactccacg ttaatattca
    19501 tagttgctag tgattagcta accaggtaag aggtcgggac gaaaatcctg gccttgtttt
    19561 tattagtaac catactttaa tgcgataatt gattggactt ttgtcaagga gatgtgtatg
    19621 tttgaagtag aagaatggct ccatagtcgg attggtttaa attttcgatc aggtttgggc
    19681 cgaatgcagc aagcggtgga tttgttagga aatcctgagc agtcttaccc tattatccac
    19741 gtaacaggga ctaatgggaa aggatctacc attgctttta tgagggaatt atttatgggg
    19801 catggcaaaa aagttgcgac ctttacctcc cctcatatcg tctctatcaa tgaccgaatc
    19861 tgcattaatg ggcaacctat agcagacgca gactttatcc gtttggctga tcaggtcaag
    19921 gagatggaga aaacgcttct gcaaactcct gaccagttgt ccttttttga attgctgacc
    19981 ttggttgctt ttctttattt tagggagcaa gaggtggatt tggttttatt agaagtggga
    20041 attggtggct tacttgacac gaccaatgtg gtaactggag agcttgctgt catcacctcc
    20101 attgggcttg atcatcaaga aaccttgggt gatagtctag aagcaattgc agagcagaaa
    20161 gctggtattt tcaaggctgg taagaaggca gtgattgcga aattgcctcc agaagctagg
    20221 cttatctgtc agaaaaaagc cgaatcttta gctgttaacc tttatcaggc aggtcaagat
    20281 tttttaatgc tgaatggtga tttttcaagc tctttactaa atctttcaca gctggacata
    20341 ggcttagaag gagtctatca gcaggagaat gcagccttgg cgttgcaaac ttttcttctt
    20401 tttatgggag aaggaaagga agctgttgat gaacaggctg taagaaaggc cttggaacag
    20461 acccattggg ctggtcgctt ggagcgtatt cgcccacaga tttacttgga tggtgctcat
    20521 aacctccctg ccttgactcg cttggttgag tttatcaaag aaaaagagca ggaaggttat
    20581 cgacctcaaa tcctctttgg atccttgaaa cgtaaggatt atcaagggat gttgggttat
    20641 ctgactgaaa atttacctca agtggaactc aagatgactg gctttgacta tcaagggtct
    20701 ttggacgaga cagatgtaac aggttaccat gtaattccct cttaccgaga atttatcagc
    20761 agtttcgaag aaagggcaga cgctcaagac ttgctgttcg taacagggtc tctctatttt
    20821 atctcagaag tacggggcta cctgctggac cgtgagcaga taaattgacc cccttttttg
    20881 aacttgttat actaggggaa ttgctggtta gagaaaattt ctctaaattg gtagcagaaa
    20941 ggaaatccat catgaaatta aaaagattca cactttctct tgcttctcta gcaagtttta
    21001 gtctcttagt agcttgttca caaagagctc aacaggttca acagcctgct caacagaata
    21061 ccaatactgc aaatgcaggg ggtaaccaaa atcaagcggc tccagtacaa aaccaacctg
    21121 ttgctcagcc gaccgatatt gatgggactt atactggtca ggatgacgga gaccgtatca
    21181 ctttagtggt aactggaacg actggtacat ggactgagct cgaatctgac ggggatcaga
    21241 aagtcaaaca ggttacattt gattcagcaa atcaacgcat gattattggc gatgatgtca
    21301 aaatttacac tgtaaacggt aatcaaatcg tcgtagatga tatggataga gacccatcgg
    21361 accaaatcgt tttaactaaa taagactatg caagtagaaa gggctggatt tttcagcctt
    21421 tttactttta cctagaaaat aatcataaat acttgccttc taccgaatac ctgcgttata
    21481 ctagtatcaa ttacttgttt ttagcattca aactatcaag gaggggatat gaaatatagg
    21541 aaatttcaat tattgatgtc caagtatggc tttagtcttt cgattatgtt acttgaactt
    21601 tgtcttgttt ttggtctctt tctttattta ggacgcatgg ctcccatttt atggattact
    21661 gtcctcattc tactgagtat catcacaatc atttcgatag tcaaccgtaa tacgactcct
    21721 gagaataagg taacctggtt gttagtagcc tttgtgccag tatttggtcc cttgctctat
    21781 ctgatgtttg gtgaaaggcg attgtccaaa aaagaaatca aacaactgaa gaagctaggc
    21841 tctatgcatt tccaagaagc aaatagccag ctactaaaag agaaattaaa agaaagtgac
    21901 aaggcagctt atggagtcat caagtcctta ttgagtatgg ataccaatgc tgacatctat
    21961 gatcaaactg cctctacatt ttttcctaac ggagaagcta tgtggaaaaa gatggtagaa
    22021 gatcttaaaa aggctgagaa atttattttc ttggaatatt acattataga agaaggtttg
    22081 atgtggaatc gcatactaga tatactagag caaaaggtag ctcagggtgt agaggttaag
    22141 atgctctatg atgatatcgg ctgtatggct actttaacag gagattatgc acatcgactt
    22201 cgtcagctgg gcatcgaggc ccataaattc aataaagtta ttcctcgttt gacagtggct
    22261 tataataaca gagatcatag aaaaatattg attgttgatg gtcagatagc ctatactggt
    22321 ggggtcaatc tggcagatga gtacattaac cacgtcgaga gatttggtta ttggaaggat
    22381 agtggaattc gcttagacgg actagcagta aaagctctga cacgcttatt tttgaccact
    22441 tggtacatta atcgaggaga aattagtgat tttgatcaat atcatttaga aaatcattct
    22501 atcccgagtg acggtttaac cattccatac ggaagtggac ccaagccaat ttttcgagcg
    22561 caggtaggga aaaaagttta tcagagttta atcaatcaag caacagaatc ggtctatatt
    22621 acgacacctt atttgattat agattatgat ttaacagaga caatcaaaaa tgcagctatg
    22681 agaggggtcg atgttcgaat tatcacccct tacataccag ataagaagtt cattcagtta
    22741 gtcacgagag gagcttatcc cgaccttctt tctgctggtg ttcggattta tgagtatagt
    22801 ccaggtttta ttcatagtaa gcagatgttg gtagacgaag attttgcggt ggtggggaca
    22861 atcaatctcg actaccggag cttggtacac cattatgaaa atgcagtctt actctataaa
    22921 actccttcta taagggaaat cgcccgagat tttcgaaata tatttgcaga ttctcaggaa
    22981 gtctatcctc attctatcaa aacgagctgg tatcaaaagc ttgtaaaaga aatcgcccag
    23041 ctattcgccc ctatcttata agaaatctag aacttaggat acaaaccaga cctatttttc
    23101 ttgtctttta cgaatagaaa gaaagaggag gggaattcat gctaactaag gaagaagtga
    23161 atgatatgat tgagttcaaa cagacacatt tacaccgctt tcgggaaatt gaaacctttg
    23221 tgaaactatg caaaaaatca ctcaaacagc catcgcaagc tgacaatcct aggacttttt
    23281 gatatactaa gacagataaa ttgaacagga gaaacgatat gagtgtgtat ggtagagtag
    23341 aagaagttca taaggagaat cgtgaacctc tagaatacca aatcgaacaa gaatcgcatc
    23401 atcgtgaatc aagtcgtctt cctttggtga aaattttact atggagtacg cttgtaacgg
    23461 ggataactct aggagtgccg ctattactcg acttaatgag tgcacaagaa gtgcaggatt
    23521 tttatgcagg ttgggcgctt catcagacag ggaagattta cagcgactat tatggaagtc
    23581 aaggtttgct ttattatttg ctgacttacg tgagtcaggg cggatttttc tttgccatct
    23641 ttgagtggtt agccttggta gcaggaggat ttttcctttt tagatcagcg gacaccttga
    23701 cagagcaagg agaccaagct ggacatctgg tgactatttt ttacatgcta gttacaggtc
    23761 ttgcttttgg tggaggctat gcgactcttt tagcgcttcc tttcttattc gcagccttta
    23821 gtttagttgc ggcttaccta agcaatccaa gccatgataa gggatttgta cggattgggc
    23881 tagctttggc aggcggattt ttctttgctc ccttatcatc gctcctgttt attgctgtag
    23941 tgagtttagg cttgttggtc tttaaccttg ggcatagacg ttttgtgcat gggttttatc
    24001 agtttcttgc agtggcttta ggtttttcac ttgtctttta tccaactgcc tactatagtg
    24061 ctgcaacagg aagttttggg gatgcgatta gtggtattcg ttatcctatt gacagtattc
    24121 gctttgattt tacttctaaa attttagaga atatgttttt ttatggcttg ttgtcccttg
    24181 gtttgggatt tgtggtttgt atctttttag gtctctttca atccaaaccc tttaaactat
    24241 acgtcatttc ggttcctgca agtcttgtgg taattttagg tttgatttta cttttctttt
    24301 cacaagagcc tctgcacgct tcttatttga tggtcgtctt ccctgttttc ctacttttat
    24361 tggtaaccaa tattaagagt caacagaggg ggcgtagtgc tagaagaagc cgaagagaaa
    24421 cgccagttag cctatggagt cgtttcttca aaggaaatct atatctgcta gtttttgggt
    24481 ttgtctatct tttgtctgtt ccttttttga tgaagtttgt cctttatcca gtaccttatc
    24541 aagaacgtaa tcgtcttgct gatttggtaa aagaggagac aaatacggaa gatgctatct
    24601 atgcatggga tgatactgcg actctttatc gtaagagtga gcgcttgtcg ccatcggcga
    24661 ttttgtcccc gttgcactat acagcaactg aggaaaatcg taataagtta cttaatgact
    24721 tgaaagaaaa acaacctaag gtgattgtgg taaatgacaa ggtggtagtc tggtctgaag
    24781 tggaaacact cttaaaagaa aattaccaac aagtaaagac tgattactca gagtttaaag
    24841 tctataaaat taaataacca aatcaatatc ttgtgtattt ttaaaaattt taggattttt
    24901 aacacaagat attgattttt ctttttagag tggtataata ctttttagaa agaacatttt
    24961 agaaaagagc atgcgtatga ttgcactaga agaaaaaatt acaattttgc caactctctt
    25021 cgtcgagaaa cgagatggga gacgtgttgt atttgatgtg gacaagattg acaaggctct
    25081 ccacaaggcg gctgacaagg ttatggatgt gacacccctg gttgaaaaac gcctcaatga
    25141 tctgactgag cgaattatta cagaaattca tagtcgcttt ccacagggaa ttaagattta
    25201 cgaaattcaa aatatcgtag aacatgaact ccttgaagcc aaagaatatg cgctggctga
    25261 ggagtatatt acttatcgga cacagaggga ttttgagcgc tcaaaagcga cggatatcaa
    25321 ctttagtatt cataaacttc tcaacaaaga ccagacagtt gtcaatgaaa atgctaataa
    25381 agacagtgat gtctttaaca ctcagcgtga tttgacagca gggattgttg ggaaatcaat
    25441 cggactgcaa atgcttccta agcacgtagc caatgcccac caaaaggggg atatccacta
    25501 tcacgatttg gactacagtc cctatacccc tatgaccaac tgctgtttgg ttgattttaa
    25561 gggtatgttg gaaaatggtt ttaagattgg aaatgcagag gtagagagtc ccaagtctat
    25621 ccagactgcg acagcacaga tttctcaaat cattgccaac gttgcttcta gccagtacgg
    25681 tggctgttca gctgaccgta tcgatgaaat tttggcgcct tatgcagaga agaactatca
    25741 aaaacatctc aaagatgcag aagagtgggt attgcctgaa aaacgggaag attacgcttg
    25801 gaagaaaacg caaaaggaca tctacgatgc catgcaatct cttgagtatg aaatcaatac
    25861 tctcttcact tcaaatggac aaacaccttt tacttcgtta ggttttggtc tgggaaccag
    25921 tcgttttgaa cgagaaattc aaaaagctat tttaaacatt cgaatcaagg gtcttggttc
    25981 agaacaccgt acggctatct ttcctaaact tattttcact ctgaaaagag gccttaattt
    26041 agaggaagga actcccaact acgacatcaa gcagttggct cttgagtgtg caaccaaacg
    26101 gatgtatcca gatgttttat cttacgataa gattgttgac ttgacaggat ccttcaaggt
    26161 gcctatgggt tgccgttctt tcctccaagg atggaaggat gaaaatggtg tagaagtcaa
    26221 ttcaggtcgc atgaatctgg gtgttgtgac ggttaatctg cctcgtattg ctcttgagtc
    26281 tgaaggtgat atgaataagt tctgggaaat cttcaacgag cgaatgaata tcgcagaaga
    26341 tgctcttgtt taccgtgtcg aacgcactaa agaggcgaca ccagcaaatg ctcctattct
    26401 ttatcagtac ggtgcttttg gccatcgtct aggtaaagaa gaaagtgttg accagctctt
    26461 taagaatcgt cgtgcgaccg tttcgctggg ctatatcggc ttgtatgaag tagcgacagt
    26521 tttctttggt aacagctggg aaagtaatcc agatgctaag gaattcacgc tagacatcat
    26581 tcacgatatg aaacgccgtg tagaagagtg gtcagaccaa tatggctacc atttctctat
    26641 ctactcaaca ccatccgaaa gtctgacaga ccgtttctgc cgactagata tagacaagtt
    26701 tggctctatt cctgatatca cagacaagga atactacacc aactctttcc actacgatgt
    26761 tcgtaaaaat ccaacaccgt ttgaaaaatt ggactttgag aaagtctatc cagaagcagg
    26821 tgcgtcaggt ggtttcatcc attattgtga gtatccagtc cttcagcaaa atccaaaggc
    26881 cttggaagct gtctgggatt atgcttatga ccgtgtaggc tatctaggca ccaatactcc
    26941 gattgaccgt tgctacaagt gtgactttga aggggatttt gaaccaactg agagagggtt
    27001 tgcttgtcca aactgtggca atagcgatcc taaaacagta gacgttgtta aacggacttg
    27061 tggctatctt ggcaatcctc aagcaagacc gatggtcaac gggcgtcaca aggaaatcgc
    27121 tgcgcgtgtc aaacatatga atggttcaac gattaaaata gctgggcatc aagtaacaaa
    27181 ttagaaagaa atgaaatggg gaaatatcaa ctagacgata aggggcgcgc acaagtgacc
    27241 cgttatcacg agaaatactc taaaggtgga gctggtaaga aagaacgctt gcttagcttc
    27301 agagaacaat ttttaaacaa gaacaagaaa aaataaaagt gagagccagc tctcgctttt
    27361 ctcatagtgg gaggtaagga tggaattacg cagaccaaga ttagcggata agaaagctgt
    27421 tttagatatg atgacagagt ttgaaaagag ccaatcagcc catgatggag gattttggga
    27481 tacagagaac tttgtgtatg aagagtggtt ggaaagcaat caggaacagg aaatggggat
    27541 taatctgcct gaaggatggg ttcctgcaat tcagttagtg gctttttctg agaaaggtca
    27601 agcagttgga tttcttaatc tccggttgcg cctcagtaac tttctactag aagaaggtgg
    27661 gcacattggc tactccatcc gtccatctga aagaggcaag ggttatgcaa aagagactct
    27721 ccgtcagggc ttgcaagttg ctaaggaaaa gaacatcaag aaagctctgg tgacctgtag
    27781 cgtgaataat cctgctagca gagcagtcat tctagcaaat ggtggaatat ttgaggatgc
    27841 tcacaatgga gtcgagcgtt attggataga ggtagcgaat gaataatcca aaaccacaag
    27901 aatggaaaag cgaggaactt agtcaaggtc gtatcattga ctacaaggcc tttaactttg
    27961 tggacggcga aggcgtgcgc aactctctct atgtatcagg ctgtatgttt cactgcgagg
    28021 gatgttataa tgttgcgact tggtctttta atgctggcat tccctataca gcagaattag
    28081 aagagcagat catggcagac cttgcccaac cctatgttca aggcttgact ttgctgggag
    28141 gggaaccttt tctcaatact ggcattctct tgcctctagt taaacgcatc cgaaaggaat
    28201 tgtcagacaa ggacatttgg tcctggacgg gctacacttg ggaagaaatg atgttggaaa
    28261 ctccagataa actggaactc ttatcattga ttgacattct tgtcgatgga cgatacgatc
    28321 gaactaagag aaatctcatg ctccagtttc gaggttcgtc caaccaacga attatcgatg
    28381 tgcaaaaatc gctcaaaagt gggcaagtag tgatttggga caagctcaat gacggaaaag
    28441 aaagctatga acaggtgaag agagaatgaa gaaaaaagac ttagtagatc aactagtctc
    28501 agagatcgag acggggaaag tcaggacact gggaatatac ggtcatggag cttcaggtaa
    28561 atcaacctct gcacaggaat tgtaccaagc tttagattct actacagtaa atttgctaga
    28621 aacagatccc tatatcacct caggacgcca tctggtagta cccaaggacg cgccgaatca
    28681 aaaggtgaca gccggtctgc cagtggcgca tgaactggag agtttgcaga gagatatcct
    28741 tgccttgcag gcgagtatgg atgtcttgac aattgaagaa ccttggaagg ctagtgaggt
    28801 cttgtctgga gccaaaccaa ttttgattgt cgaagggatg tctgttggct ttctacccaa
    28861 ggaactcttt gaaaaaacca tctgtttcta cacggatgag gagaccgaat taaagcgacg
    28921 ccttgctaga gatacgactg tgagaaatcg tgatgcatcc tttatattgg ctagccatca
    28981 gatgagacgg gagcagtatc tgcgatacta taaagaaact gagtctaagg cggatatctt
    29041 agtggatcaa tcagaagata aatttgatgt caagaggact taacttatgt atagaagaaa
    29101 tatagaagaa aatccctaat tttagaaaga aaaagtagga aaacagtttc atcccaaaaa
    29161 aacgaaaaaa cctaacaaat cccttgcaat cgcaggggat ttgtgttatt ctattatggt
    29221 gctgtaaatt acagccttag ttttgatgca agaggttgcg acacgctcgg ttgcattgcc
    29281 acgcaacacc gtgtcggttt tcttgtggag ctagcctatt atcttaaata gacgaaaagg
    29341 agaaaaagat ggcaaacaaa aaaatccgta tccgtttgaa agcttacgaa caccgtacgc
    29401 ttgacacagc ggctgcaaaa atcgtagaat cagctactcg tacaggtgca caagttgcgg
    29461 gtccaatccc acttccaact gaacgtagcc tctacacaat cattcgtgcg actcacaaat
    29521 acaaagactc tcgcgaacaa tttgaaatgc gtacacacaa acgtttgatc gatatcgtta
    29581 acccaactca aaaaacagtt gatgccttga tgaaattgga tcttccaagt ggtgtaaacg
    29641 tagaaatcaa actttaatct aaaatataag agagcagagg ctggtgtttc aatctaattg
    29701 aacacgggct aaacttggtg tgaaaaagat aaacttcctc gcgtctgcta gacactgtgt
    29761 cagtttccta ttttcacgtt gagtttgacg ccctttgtat cttagacttg agcataaaaa
    29821 acgctcgtta aaaacttttt gaataaaaaa tatagaaaag gaactatttt ctcatgacaa
    29881 aaggaatctt agggaaaaaa gtgggaatga ctcaaatctt cactgaagct ggcgaattga
    29941 tccctgtaac agttattgaa gcaactccaa acgttgttct tcaagttaaa actgttgaaa
    30001 cagacggata caacgctatc caagttggtt tcgatgacaa acgcgaagta ttgagcaaca
    30061 aacctgctaa aggacatgta gcgaaagcta acacggctcc taagcgcttc attcgtgaat
    30121 tcaaaaacgt tgaaggcttg gaagttggtg ctgaaattac agttgaaaca ttcgcagctg
    30181 gagacgttgt tgacgtaact ggtacttcta aaggtaaagg tttccaaggt gttatcaaac
    30241 gccacggaca atcacgtgga ccaatggctc acggttctcg ttaccaccgt cgtccaggtt
    30301 ctatgggacc tgttgcacct aaccgcgtat tcaaaggtaa aaaccttgca gggcgtatgg
    30361 gtggcgaccg cgtaacaatt caaaaccttg aagttgtaca agttgttcca gaaaagaacg
    30421 ttatccttat caaaggtaac gtaccaggtg ctaagaaatc tcttatcact atcaaatcag
    30481 cagttaaagc tggtaaataa taaagaaagg ggaaatcagt cacaatggca aacgtaacat
    30541 tatttgacca aactggtaaa gaagctggcc aagttgttct taacgatgca gtatttggta
    30601 tcgaaccaaa tgaatcagtt gtgtttgatg taatcatcag ccaacgcgca agccttcgtc
    30661 aaggaacaca cgctgttaaa aaccgctctg cagtatcagg tggtggacgc aaaccgtggc
    30721 gtcaaaaagg aactggacgt gctcgtcaag gttctatccg ctcaccacaa tggcgtggtg
    30781 gtggtgttgt cttcggacca actccacgtt catacggcta caaacttcca caaaaagttc
    30841 gtcgcctagc tcttaaatca gtttactctg aaaaagttgc tgaaaacaaa ttcgtagctg
    30901 tagacgctct ttcatttaca gctccaaaaa ctgctgaatt tgcaaaagtt cttgcagcat
    30961 tgagcatcga ttctaaagtt cttgttatcc ttgaagaagg aaatgaattc gcagctcttt
    31021 cagctcgtaa ccttccaaac gtgaaagttg caactgctac aactgcaagt gttcttgaca
    31081 tcgcaaatag cgacaaactt cttgtcacac aagcagctat ctctaaaatc gaggaggttc
    31141 ttgcataatg aatttgtatg atgttatcaa aaaacctgtc atcactgaaa gctcaatggc
    31201 tcaacttgaa gcaggaaaat atgtatttga agttgacact cgtgcacaca aacttttgat
    31261 caagcaagct gttgaagctg ctttcgaagg tgttaaagtt gccaatgtta acacaatcaa
    31321 cgtaaaacca aaagctaaac gtgttggacg ttacactggt tttactaaca aaactaaaaa
    31381 agctatcatc acacttacag ctgattctaa agcaatcgag ttgtttgctg ctgaagctga
    31441 ataatctaag gaggaaatat cgtgggaatt cgtgtttata aaccaacaac aaacggtcgc
    31501 cgtaatatga cttctttgga tttcgctgaa atcacaacaa gcactcctga aaaatcattg
    31561 cttgttgcat tgaagagcaa ggctggtcgt aacaacaacg gtcgtatcac agttcgtcac
    31621 caaggtggtg gacacaaacg tttctaccgt ttggttgact tcaaacgtaa taaagacaac
    31681 gttgaagcag ttgttaaaac aatcgagtac gatccaaacc gttctgcaaa catcgctctt
    31741 gtacactaca ctgacggtgt gaaagcatac atcatcgctc caaaaggtct tgaagtaggt
    31801 caacgtatcg tttcaggtcc agaagcagat atcaaagtcg gaaacgctct tccacttgct
    31861 aacatcccag ttggtacttt gattcacaac atcgagttga aaccaggtcg tggtggtgaa
    31921 ttggtacgtg ctgctggtgc atctgctcaa gtattgggtt ctgaaggtaa atacgttctt
    31981 gttcgtcttc aatcaggtga agttcgtatg attcttggaa cttgccgtgc tacagttggt
    32041 gttgtcggaa acgaacaaca tggacttgta aaccttggta aagcaggacg tagccgttgg
    32101 aaaggtatcc gcccaacagt tcgtggttct gtaatgaacc ctaacgatca cccacacggt
    32161 ggtggtgaag gtaaagcacc agttggtcgt aaagcaccat ctactccatg gggcaaacct
    32221 gctcttggtc ttaaaactcg taacaagaaa gcgaaatctg acaaacttat cgttcgtcgt
    32281 cgcaacgaga aataatatta aactagtcgc ttaagcaact agtaaatccg ccagctcggt
    32341 agcgctccat aggagtgcaa gccgctgtgg tacaacattt aaaggagaaa atataaaaat
    32401 gggacgcagt cttaaaaaag gacctttcgt cgatgagcat ttgatgaaaa aagttgaagc
    32461 tcaagctaac gacgaaaaga aaaaagttat taaaacttgg tcacgtcgtt caacgatctt
    32521 cccaagtttc attggttaca ctattgcagt ttatgacgga cgtaaacacg tacctgttta
    32581 catccaagaa gacatggtag gccacaaact tggtgaattt gcaccaactc gtacttacaa
    32641 aggtcacgct gcagacgaca agaaaacacg tagaaaataa ggagaacata aatggcagaa
    32701 attacttcag ctaaagcaat ggctcgtaca gtacgtgttt cacctcgtaa atcacgtctt
    32761 gttcttgata acatccgtgg taaaagcgta gccgatgcaa tcgcaatctt gacattcact
    32821 ccaaacaaag ctgctgaaat catcttgaaa gttttgaact cagctgtagc taacgctgaa
    32881 aacaactttg gtttggataa agctaacttg gtagtatctg aagcattcgc aaacgaagga
    32941 ccaactatga aacgtttccg tccacgtgcg aaaggttcag cttcaccaat caacaaacgt
    33001 acagctcaca tcactgtagc tgttgcagaa aaataaggag gtaaaatcgt gggtcaaaaa
    33061 gtacatccaa ttggtatgcg tgtcggcatc atccgtgatt gggatgccaa atggtatgct
    33121 gaaaaagaat acgcggatta ccttcatgaa gatcttgcaa tccgtaaatt cgttcaaaaa
    33181 gaacttgctg acgcagcagt ttcaactatt gaaatcgaac gcgcagtaaa caaagttaac
    33241 gtttcacttc acactgctaa accaggtatg gttatcggta aaggtggtgc taacgttgat
    33301 gcactccgtg caaaacttaa caaattgact ggaaaacaag tacacatcaa catcatcgaa
    33361 atcaaacaac ctgatttgga tgctcacctt gtaggtgaag gaattgctcg tcaattggag
    33421 caacgtgttg ctttccgtcg tgcacaaaaa caagcaatcc aacgtgcaat gcgtgctgga
    33481 gctaaaggaa tcaaaactca agtatcaggt cgtttgaacg gtgcagatat cgcccgtgct
    33541 gaaggatact ctgaaggaac tgttccgctt cacacacttc gtgcagatat cgattacgct
    33601 tgggaagaag cagatactac atacggtaaa cttggtgtta aagtatggat ctaccgtggt
    33661 gaagttcttc cagctcgtaa aaacactaaa ggaggtaaat aaccaatgtt agtacctaaa
    33721 cgtgttaaac accgtcgtga attccgtgga aaaatgcgcg gtgaagcaaa aggtggaaaa
    33781 gaagtagcat tcggtgaata cggtcttcaa gctacaacta gccactggat cactaaccgc
    33841 caaatcgaag ctgctcgtat cgccatgact cgttacatga aacgtggtgg taaagtttgg
    33901 attaaaatct tcccacacaa atcatacact gctaaagcta tcggtgtgcg tatgggatct
    33961 ggtaaagggg cacctgaagg ttgggtagca ccagttaaac gtggtaaagt gatgttcgaa
    34021 atcgctggtg tatctgaaga gattgcacgt gaagcgcttc gacttgctag ccacaaattg
    34081 ccagttaaat gtaaattcgt aaaacgtgaa gcagaataag gagaaggcat gaaacttaat
    34141 gaagtaaaag aatttgttaa agaacttcgt ggtctttctc aagaagaact cgcgaagcgc
    34201 gaaaacgaat tgaaaaaaga attgtttgaa cttcgtttcc aagctgctac tggtcaattg
    34261 gaacaaacag ctcgcttgaa agaagttaaa aaacaaatcg ctcgtatcaa aacagttcaa
    34321 tctgaagcga aataatagac tagggaagga gaaatttcaa tggaacgcaa taatcgtaaa
    34381 gttcttgttg gacgtgttgt atctgacaaa atggacaaga caatcacagt tgtagttgaa
    34441 acaaaacgta accacccagt ctatggtaaa cgtattaact actctaaaaa atacaaagct
    34501 catgatgaaa acaatgttgc caaagaaggc gatatcgtac gtatcatgga aactcgcccg
    34561 ctttcagcta caaaacgttt ccgtcttgta gaagttgttg aagaagcggt catcatctaa
    34621 tcaaacctga aaggagaaaa ctgaaatgat tcaaacagaa actcgtttga aagtcgcaga
    34681 caacagcggt gctcgcgaaa tcttgactat caaagttctt ggtggttcag gacgtaaatt
    34741 tgcaaacatc ggtgatgtta tcgtggcatc tgtaaaacaa gctactcctg gtggtgcggt
    34801 taaaaaaggt gacgttgtta aagcagttat cgttcgtact aaatcaggtg ctcgtcgtgc
    34861 tgatggttca tacatcaaat ttgacgaaaa cgcagcagtt atcatccgtg aagacaaaac
    34921 tcctcgcgga acacgtatct ttggcccagt tgcacgtgaa ttgcgtgaag gtggcttcat
    34981 gaagatcgtg tcacttgctc cagaagtact ttaattttta ggaacaaact agtcccctag
    35041 cttcaagcta gggtgccctt atgggcgtaa gaaaaatcaa ggagaaacct aatgtttgta
    35101 aaaaaaggcg acaaagttcg cgtaatcgct ggtaaagata agggaacaga agctgttgtc
    35161 cttactgccc ttccaaaagt aaacaaagtt atcgttgaag gtgttaacat tgttaagaaa
    35221 caccaacgtc caactaacga gcttcctcaa ggtggtatca tcgagaaaga agcagctatc
    35281 cacgtatcaa acgttcaagt tttggacaaa aatggtgtag ctggtcgtgt tggttacaaa
    35341 tttgtagacg gtaaaaaagt tcgctacaac aaaaaatcag gcgaagtgct tgattaatca
    35401 cgaaggaaag gagaagtata atggcaaatc gtttaaaaga aaaatatctt aatgaagtag
    35461 ttcctgcttt gacagaacaa ttcaactact catcagtgat ggctgtgcct aaagtagata
    35521 agattgtttt gaacatgggt gttggtgaag ctgtatcaaa cgctaaaagc cttgaaaaag
    35581 ctgctgaaga attggcactt atctcaggtc aaaaaccact tatcactaaa gctaaaaaat
    35641 caatcgccgg cttccgtctt cgtgaaggtg ttgcgatcgg tgcaaaagtt acccttcgtg
    35701 gtgaacgtat gtacgaattc ttggataaat tggtatcagt ttcacttcca cgtgtacgtg
    35761 acttccacgg tgtcccaaca aaatcatttg atggacgcgg gaactacaca cttggtgtga
    35821 aagaacaatt aatcttccca gaaatcaact tcgatgacgt tgacaaaact cgtggtcttg
    35881 acatcgttat cgtaacaact gctaacactg acgaagagtc acgtgcattg cttacaggcc
    35941 ttggaatgcc ttttgcaaaa taatatagga ggtaaatcta atggctaaaa aatcaatggt
    36001 agctagagag gctaaacgcc aaaaaattgt tgaccgttat gctgaaaaac gtgctgcatt
    36061 aaaggcggca ggggactacg aaggtttatc taaattacct cgcaacgcct caccgactcg
    36121 tttacataat cgttgtaggg ttacggggcg cccacattca gtttaccgca aatttggtct
    36181 gagtcgtatc gcttttcgcg aacttgcgca taaaggtcaa attcctggtg taacaaaggc
    36241 atcttggtaa tttaagatat caagagcgtc aaaactccaa gtaaaaatag gaaacttgac
    36301 gaagaaacta aagtttctag gaaagtttat ctttttcaca cagagtttag cccgggttca
    36361 attgggcttg ccaatttgaa cacgagctac agctttggca aaaaagacca atttgctttg
    36421 gagcattgct tctgcattaa attgtctatt tttgctcgtg ctgttacgct ctttgtatca
    36481 tgtattaact agcaagtgca acttgcaaac tactagtaag aggagaaaaa caaaatggtt
    36541 atgactgacc caatcgcaga cttcctaact cgtattcgta atgctaacca agctaaacac
    36601 gaagtacttg aagtacctgc atcaaacatc aaaaaaggga ttgctgaaat ccttaaacgc
    36661 gaaggttttg taaaaaacgt tgaaatcatt gaagatgaca aacaaggcgt catccgtgta
    36721 tttcttaaat acggaccaaa tggtgagaaa gttatcacta acttgaaacg tgtttctaaa
    36781 ccaggacttc gtgtctacaa aaaacgtgaa gaccttccaa aagttcttaa cggacttgga
    36841 attgccatcc tttcaacttc tgaaggtttg cttactgata aagaagcacg ccaaaagaat
    36901 gttggtggtg aggttatcgc ttacgtttgg taaaatcaag atacaaagct cgtaaagaac
    36961 aaagcaaaat taggaagttg gagaagtttg tttacaaaca agccaactta tctattttgc
    37021 acagttctta gagcgtgttc agttcagctc ttgaactaaa taagtatctg aaccccgtga
    37081 aaactggccg ttctggcctg acaatttaac aggagaaaat aaacatgtca cgtattggta
    37141 ataaagttat cgtgttgcct gctggtgttg aactcgctaa caatgacaac gttgtaactg
    37201 taaaaggacc taaaggagaa cttactcgtg agttctcaaa agatattgaa atccgtgtgg
    37261 aaggtactga agtaactctt caccgtccaa acgattcaaa agaaatgaaa actatccacg
    37321 gaactactcg tgcccttttg aacaacatgg ttgttggtgt atcagaagga ttcaagaaag
    37381 aacttgaaat gcgtggggtt ggttaccgtg cacagcttca aggatctaaa cttgttttgg
    37441 ctgttggtaa atctcatcca gacgaagttg aagctccaga aggaattact tttgaacttc
    37501 caaacccaac aacaatcgtt gttagcggaa tttcaaaaga agtagttggt caaacagctg
    37561 cttacgtacg tagccttcgt tcaccagaac catataaagg taaaggtatc cgttacgttg
    37621 gtgaattcgt tcgccgtaaa gaaggtaaaa caggtaaata atgttgagtg gttgatcatc
    37681 aaccaccaac ctattttcca actttgtgca tagcacacga tttaaaacta aagaggtgaa
    37741 aactgtgatt tcaaaaccag ataaaaacaa actccgccaa aaacgccacc gtcgcgttcg
    37801 cggaaaactc tctggaactg ctgatcgccc acgtttgaac gtattccgtt ctaatacagg
    37861 catctacgct caagtgattg atgacgtagc gggtgtaacg ctcgcaagtg cttcaactct
    37921 tgataaagaa gtttcaaaag gaactaaaac tgaacaagcc gttgctgtcg gtaaactcgt
    37981 tgcagaacgt gcaaacgcta aaggtatttc agaagtggtg ttcgaccgcg gtggatatct
    38041 atatcacgga cgtgtgaaag ctttggctga tgcagctcgt gaaaacggat tgaaattcta
    38101 ataggaggac actagaaaat ggcatttaaa gacaatgcag ttgaattaga agaacgcgta
    38161 gttgctgtca accgtgttac aaaagttgtt aaaggtggac gtcgtcttcg tttcgcagct
    38221 cttgttgttg ttggtgacca caatggtcgc gtaggatttg gtactggtaa agctcaagaa
    38281 gttccagaag caatccgtaa agcagtagat gatgctaaga aaaacttgat cgaagttcct
    38341 atggttggaa caacaatccc acacgaagtt ctttcagaat tcggtggagc taaagtattg
    38401 ttgaaacctg ctgtagaagg ttctggagtt gccgctggtg gtgcagttcg tgccgttgtg
    38461 gaattggcag gtgtggcaga tattacatct aaatcacttg gttctaacac tccaatcaac
    38521 attgttcgtg caactgttga aggtttgaaa caattgaaac gcgctgaaga aattgctgcc
    38581 cttcgtggta tttcagtttc tgatttggca taagaaaggg gataaaatgg ctcaaattaa
    38641 aattactttg actaagtctc caatcggacg cattccatca caacgtaaaa ctgttgtagc
    38701 acttggactt ggcaaattga acagctctgt tattaaagaa gataacgctg ctatccgtgg
    38761 tatgattaca gcagtatctc acttagtaac agttgaagaa gtaaactaat gaagttttag
    38821 gggatgtgca ctgtaccatc ccctaaaact agatatagtc atctatgatg acatcgtata
    38881 ggcgagttga tgggggagac aaccttttct cccttatcgg cgctagcatt ttacaaaaga
    38941 ggagaaaata aaaatgaaac ttcatgaatt gaaacctgca gaaggttctc gtaaagtacg
    39001 taaccgcgtt ggtcgtggta cttcatcagg taacggtaaa acatctggtc gtggtcaaaa
    39061 aggtcaaaaa gctcgtagcg gtggcggagt tcgccttggt tttgaaggtg gacaaactcc
    39121 attgttccgt cgtcttccaa aacgtggatt cactaacatc aacgctaaag aatacgcaat
    39181 tgtgaacctt gaccaattga acgtctttga agatggtgct gaagtaactc cagttgttct
    39241 tatcgaagca ggaattgtta aagctgaaaa gtcaggtatt aaaattcttg gtaacggtga
    39301 gttgactaag aaattgactg tgaaagcagc taaattctct aaatcagctg aagaagctat
    39361 cactgctaaa ggtggttcag tagaagtcat ctaagagagg tgacctatgt tttttaaatt
    39421 attaagagaa gctcttaaag tcaagcaggt tcgatcaaaa attttattta caatttttat
    39481 cgttttggtc tttcgtatcg gaactagcat tacagttcct ggtgtgaatg ccaatagctt
    39541 gaatgcttta agtggattat ccttcttaaa catgttgagc ttggtgtcgg ggaatgccct
    39601 aaaaaacttt tcgatttttg ccctaggagt tagtccctat atcaccgctt ctattgttgt
    39661 ccaactcttg caaatggata ttttacccaa gtttgtagag tggggtaaac aaggggaagt
    39721 aggtcgaaga aaattgaatc aagctactcg ttatattgct ctagttctcg cttttgtgca
    39781 atctatcggg attacagctg gttttaatac cttggctgga gctcaattga ttaaaactgc
    39841 tttaactcca caagtttttc tgacgattgg tatcatctta acagctggta gtatgattgt
    39901 cacttggttg ggtgagcaaa ttacagataa gggatacgga aacggtgttt ccatgattat
    39961 ctttgccggg attgtttctt caattccaga gatgattcag ggcatctatg tggactactt
    40021 tgtgaacgtc ccaagtagcc gtatcacttc atctatcatt ttcgtaatca ttttgattat
    40081 tactgtattg ttgattattt actttacaac ttatgttcaa caagcagaat acaaaattcc
    40141 aatccaatat actaaggttg cacaaggtgc tccatctagc tcttaccttc cgttaaaggt
    40201 aaaccctgct ggagttatcc ctgttatctt tgccagttcg attactgcag cgcctgcggc
    40261 tattcttcag tttttgagtg ccacaggtca tgattgggct tgggtaaggg tagcacaaga
    40321 gatgttggca actacttctc caactggtat tgccatgtat gctttgttga ttattctctt
    40381 tacattcttc tatacgtttg tacagattaa tcctgaaaaa gcagcagaga gcctacaaaa
    40441 gagtggtgcc tatatccatg gagttcgtcc tggtaaaggt acagaagaat atatgtctaa
    40501 acttcttcgt cgtcttgcaa ctgttggttc cctcttcctt ggtgtgattt ccattttacc
    40561 gattgcagct aaagatgtat ttggtctttc tgatgttgtt gcctttggtg gaacaagtct
    40621 cttgatcatt atctctacag gtatcgaagg aatcaagcaa ttggaaggtt acctattgaa
    40681 acgtaagtat gttggtttta tggacagaac agaataaaag tatttactga atcagtaaat
    40741 gctgagagag tggaggttta aactctgaca tttgtaagag ttggatctcc cctcttctat
    40801 tttgttttta aataggggtg aaaagacttt ttgcttctat ttaaaaataa aataaggaga
    40861 tcaaatcatg aatcttttga ttatgggctt acctggtgca ggtaagggaa ctcaagcagc
    40921 aaaaatcgta gaacaattcc atgttgcaca tatctcaaca ggtgatatgt tccgcgctgc
    40981 aatggcaaat caaactgaaa tgggtgttct tgctaagtca tatattgaca agggtgaatt
    41041 ggttcctgac gaagttacaa atggaatcgt aaaagaacgt ctttcacaag atgatattaa
    41101 agaaacagga ttcttattgg atggttaccc acgtacaatt gaacaagctc atgccttgga
    41161 caaaacattg gctgaacttg gcattgaact agaaggtgtt atcaatattg aagtgaaccc
    41221 tgacagcctt ttggaacgtt tgagtgggcg tatcatccac cgcgtaactg gagaaacttt
    41281 ccacaaggtc tttaacccac cagttgacta taaagaagaa gattactacc aacgtgaaga
    41341 tgataagcct gagacagtaa aacgtcgttt ggatgttaat attgctcaag gagaaccaat
    41401 cattgctcac taccgtgcca aaggtttggt tcatgacatc gaaggtaatc aaaatatcaa
    41461 tgatgtcttc tcagatattg aaaaagtatt gacaaatttg aaataaagcg tttttcacac
    41521 ttgcaaaaat ccgctacaaa tgttatactg agatagtctg acttataatt gttgtctctg
    41581 tgtctagagg catcgaatcg aaatttatgg aggtgctttt gcgtggcaaa agacgatgtg
    41641 attgaagttg aaggcaaagt agttgataca atgccgaatg caatgtttac ggttgaactt
    41701 gaaaatggac atcagatttt agcaacagtt tctggtaaaa ttcgtaaaaa ctatattcgt
    41761 attttagcgg gagatcgtgt tactgtcgaa atgagtccat atgacttgac acgtggacgt
    41821 atcacttacc gctttaaata atcgaaaaac ttggagggat aagaaatgaa agtaagacca
    41881 tcggtcaaac caatttgcga atactgtaaa gttattcgtc gtaatggtcg tgttatggta
    41941 atttgcccag caaatccaaa acacaaacaa cgtcaaggat aagatagaaa ggagaaaaca
    42001 tggctcgtat tgctggagtt gatattccaa atgacaaacg cgtagtaatc tcattgactt
    42061 atgtttatgg tatcggactt gcaacatcta agaaaatttt ggctgctgct ggaatctcag
    42121 aagatgttcg tgtacgtgat cttacatcag atcaagaaga tgctatccgt cgtgaagtgg
    42181 atgcaatcaa agttgaaggt gaccttcgtc gtgaagtaaa cttgaacatc aaacgtttga
    42241 tggaaatcgg ttcataccgt ggtatccgtc accgtcgtgg acttcctgtc cgtggacaaa
    42301 acactaaaaa caacgctcgc actcgtaaag gtaaagctgt tgcgattgct ggtaagaaaa
    42361 aataatatag gaggtaaaag tcttggctaa accaacacgt aaacgtcgtg tgaaaaagaa
    42421 tatcgaatct ggtattgctc atattcacgc tacatttaat aacactattg ttatgattac
    42481 tgatgtgcat ggtaatgcaa ttgcttggtc atcagctggt gctcttggtt tcaaaggttc
    42541 tcgtaaatct acaccattcg ctgctcaaat ggcttctgaa gctgctgcta aatctgcaca
    42601 agaacacggt cttaaatcag ttgaagttac tgtaaaaggt ccaggttctg gtcgtgagtc
    42661 agctattcgt gcgcttgctg ccgctggtct tgaagtaaca gcaattcgtg atgtgactcc
    42721 agtgccacac aatggtgctc gtcctccaaa acgtcgccgt gtataatcat cgcattacac
    42781 tgcttttcgt ttaagaggga gtaactaaat gatcgagttt gaaaaaccaa atataacaaa
    42841 aattgatgaa aataaagatt atggcaagtt tgtaatcgaa ccacttgaac gtggctacgg
    42901 tacaactctt ggtaactctc ttcgtcgtgt acttctagct tctctaccag gagcagctgt
    42961 gacatctatc aacattgatg gtgtgttaca tgagtttgac acagttccag gtgttcgtga
    43021 agacgtgatg caaatcattc tgaacattaa aggaattgca gtgaaatcgt acgttgaaga
    43081 cgaaaaaatc atcgaactgg atgttgaagg tcctgctgaa gtaacagctg gtgacatttt
    43141 gacagatagc gatattgaaa ttgtaaatcc agatcattat ctctttacaa tcggtgaagg
    43201 ttcttctcta aaagcgacta tgactgttaa cagtggtcgt ggatatgtac ctgctgatga
    43261 aaataaaaag gataatgcac cagttggaac acttgctgta gattctattt atacaccagt
    43321 tacaaaagtc aactatcaag tggaacctgc tcgtgtaggt agcaatgatg gtttcgacaa
    43381 attaaccctt gaaatcttga caaatggaac aattattcca gaagatgctt tagggctttc
    43441 agcacgtatt ttgacagaac atcttgattt gtttacaaat cttactgaga ttgctaagtc
    43501 aactgaagtg atgaaagaag ctgatactga atctgacgac cgtattttag atcgtacgat
    43561 tgaggaactg gacttgtctg tgcgttcata caactgttta aaacgtgccg gtatcaatac
    43621 tgtgcatgat ttgacagaaa aatctgaagc agagatgatg aaagtacgaa atcttggacg
    43681 caagagtttg gaagaagtga aactcaaact cattgatttg ggtcttggat taaaagataa
    43741 ataaaggagg aatacatggc ttaccgtaaa ctaggacgca ctagctcaca acgtaaagca
    43801 atgcttcgcg atttgacaac tgaccttttg atcaacgaat caatcgtgac aactgaagct
    43861 cgtgctaaag agatccgtaa aactgttgaa aaaatgatta ctctaggtaa acgtggtgat
    43921 ttgcatgcac gtcgtcaagc agctgctttc gtacgtaatg aaatcgcatc tgaaaactat
    43981 gatgaagcaa ctgataagta cacttctact acagcacttc aaaaattgtt ctcagaaatc
    44041 gcacctcgtt atgctgaacg taacggtgga tacactcgta tccttaaaac tgaaccacgt
    44101 cgtggtgatg cagcgccaat ggcgatcatc gaattagtat aaaatcatca attttgttga
    44161 gtgttatgat gatggagtct tgtgctctta gtctagctct ggtctaccgc taggatttcg
    44221 gtcctagcgg gaacactcat cataagttgg gatagtagac gcttgtttac gaaattgttt
    44281 ttttcttaag aacaacttcg taagcaggcg tttttgagta ttttcgttag aattatgcta
    44341 tactatttga aaagaatcct gtttaatgtt aaggtttctt attttaagaa gaattggagt
    44401 ttacttatga aagccattat aactgttgtt ggtaaagata aatctggaat tgttgcaggt
    44461 gtttctggta aaattgcaga attaggattg aatattgacg atatctctca aactgtcttg
    44521 gatgaatatt ttacgatgat ggctgttgta tctagtgatg aaaagcaaga ttttacctat
    44581 cttcgtaatg aatttgaagc ttttgggcaa actttgaatg taaaaatcaa tattcagagt
    44641 gcagcgattt tcgaagctat gtataatatc taggaggtca tcatggatat tagacaagtt
    44701 actgaaacca tcgccatgat tgaggagcaa aacttcgata ttagaaccat taccatgggg
    44761 atttctcttt tggactgtat cgatccagat atcaatcgtg ctgcggagaa aatctatcaa
    44821 aaaattacga caaaggcggc taatttagta gctgttggtg atgaaattgc ggctgagttg
    44881 ggaattccta tcgttaataa gcgtgtatcg gtgacaccta tttctctgat tggggcagcg
    44941 acagatgcga cggactacgt ggttctggca aaagcgcttg ataaggctgc gaaagagatt
    45001 ggtgtggact ttattggtgg tttttctgcc ttagtacaaa aaggttatca aaagggagat
    45061 gagattctca tcaattccat tcctcgcgct ttggctgaga cggataaggt ctgctcgtca
    45121 gtcaatatcg gctcaaccaa gtctggtatt aatatgacgg ctgtggcaga tatgggacga
    45181 attatcaagg aaacagcaaa tctttcagat atgggagtgg ccaagttggt tgtattcgct
    45241 aatgctgttg aggacaatcc atttatggag ggtgcctttc atggtgttgg ggaagcagat
    45301 gttatcatca atgtcggagt ttctggtcct ggtgttgtga aacgtgcttt ggaaaaagtt
    45361 cgtggacaga gctttgatgt agtagccgaa acagttaaga aaactgcctt taaaatcact
    45421 cgtatcggtc aattggttgg tcaaatggcc agtgagagac tgggtgtgga gtttggtatt
    45481 gtggacttga gtttggcacc aacccctgcg gttggagact ctgtggcacg tgtccttgag
    45541 gaaatggggc tagaaacagt tggcacgcat ggaacgacgg ctgccttggc cctcttgaac
    45601 gaccaagtta aaaagggtgg agtgatggcc tgcaaccaag tcggtggttt atctggtgcc
    45661 tttatccctg tttctgagga tgaaggaatg attgctgcag tgcaaaatgg ctctcttaat
    45721 ttagaaaaac tagaagctat gacggctatc tgttctgttg gattggatat gattgccatc
    45781 ccagaagata cgcctgctga aaccattgcg gctatgattg cggatgaagc agcaatcggt
    45841 gttatcaaca tgaaaacaac agctgttcgt atcattccca aaggaaaaga aggcgatatg
    45901 attgagtttg gtggtctatt aggaactgca cccgttatga aggttaatgg ggcttcgtct
    45961 gtcgacttca tctctcgcgg tggacaaatc ccagcaccaa ttcatagttt taaaaattaa
    46021 gaaaatagga gaaattttaa gttctattta agattagatg tgtatactat aatcattaaa
    46081 taaagacctc ctaatattat ttgaaacaga taacactgaa ttagtttgaa tttgattttc
    46141 atctaatatc tttatttaat gaactcctaa actttttcat aataatctcc ttcaaaagtc
    46201 gcctgtatgg gtggctttta ttttatcatt catgatataa tagaagcaaa cggaggacgg
    46261 aaaatggtaa aagtacgatt gtatttggta cgtcatggca agaccatgtt taacacgatt
    46321 ggtcgcgcgc aaggttggag cgatactccc ttaactgctg aaggtgaacg agggattcaa
    46381 gagttaggaa tcggtttgcg agaatctgat ctacagtttg agcgcgctta ttcgagtgat
    46441 tctggtcgta ccattcagac catgggaatt atacttgaag aacttggctt gcaggggaaa
    46501 atcccttatc gcatggacaa gcgtatcaga gaatggtgtt tcggtagttt tgatggagcc
    46561 tatgatggcg atcttttcat gggcattatt cctcgtatct ttaatgtgga ccacgttcac
    46621 caattgtctt atgctgaact ggctgagggc ttggtagagg tcgatacagc tggttgggct
    46681 gaaggttggg aaaaactcag tggccgaatc aaggaaggct ttgaaataat tgcaaaagaa
    46741 atggaagatc aaggtggagg taacgccctt gttgtcagcc atggaatgac tattggaacc
    46801 attgtttatc tgattaatgg catgcatccg catggtctag ataatggtag cgtgacgatt
    46861 cttgaatatg aggacggtca gtttagggtt gaagttgtcg gtgaccgtag ttaccgagag
    46921 ctaggacgtg agaagatgga agaaggctct atttaatcag tctagacttg cttgccatga
    46981 gctagggatt tgataagaat atcaagataa gaaaaaacag ccgagggtac tcctttcggc
    47041 tgtttttgat gtggaaaact aaagtgtaat gctattgctt ttagagattt tcataaacaa
    47101 gagcaaggaa cctactgtta gaacagtcag gatagttgac aaggctgcgg ctacaccgta
    47161 atttcctctg agaacctctg tataaatagc tacagtcatt gttcttgttt tgacattgta
    47221 gaggaggata gaggatagaa gtagagagtt ctgaaatcag tgtgacccaa gataggatgg
    47281 ctccagaaat aatacccgat agcatcattg gagttgtaat cttagcaaag gtattgagac
    47341 gactacttcc taagctttca gcagcttctt caatacttgg tgctatttgt tgtaagctag
    47401 caacagatga gcgaatagta tacggtaatc ttctgacaga tagagacata atcaagatga
    47461 aagcagtccc tgtaatcata agaaatccac ttccaaatag accagtattg aaggaagaaa
    47521 tgaaggcaat ccctagaacg gttcctggta caatataagg taccatactg aggctgtcaa
    47581 ttaagtttgt aaacaaattc cgttttctaa cggctaggta ggagataaat gtcgcaaata
    47641 gaacaactag aactaaggca atcaaaggga tacgaatggt attgaaaata gcagatccca
    47701 tacgattgaa agctagcttg taactgtttg gagaataacc tttaaaaaat accatacctg
    47761 atgtttttag gaaagaggta taaattaagt agatttgagg taaaacagag ataaagataa
    47821 ttccgtagac tgttgcataa atggcagcca tttttccttt tgtagttttt ttaggctcaa
    47881 ttggatggag cagattcatg ctgaaactgt agcggtttgc aatgtgtttt tggataagga
    47941 aaattgccaa ggcaatgata atcgccataa ttgcaaaagc agaatttcct ccaacctcgc
    48001 taataaattg ggtataaatc aggacaggga aagtccgata tccttcgcca atcaacatag
    48061 gcgttccaaa gtctgagaat gctctcataa atacaagcaa ggagctgcta gtaaggttgg
    48121 aactagaaga ggtaaaacaa ccgttacgat aggtttaaat ccgaaggacc ccatgctttc
    48181 agctgcttca agtagagaat tgtcaatact gttcattgtt ccagcaacat atagaaatac
    48241 cagtgggaat agttgcagtg taaagacaag tacaattcct ttgaatccat aaatatcgat
    48301 agctggaaga tgaagggcat ttgtcaaaaa tttagtgatg accccatttc gtcccagcaa
    48361 gagaacccag gagtaggctc ctacgaaagg agctgacatg gaagcaatga taatcaatat
    48421 ttgtagaaat ttcttcccct tgaagtcata catagagaag agataagcta atagggttcc
    48481 tacaactaag gaagtgacag tagcggtaat ggaaaccctg aaactgttga ctagtgtctc
    48541 agagtagtag gctttactaa agaaagtgac aaaattagct agtgagaatt gtccttcatg
    48601 tataagtgct tgcttgagca cggtaatgat aggataaacg agaaagacaa gataggtaag
    48661 aaagaggaag aaagaggagg ctgtccaaat atttagtttt ttacgttcca tggttgactc
    48721 cttttatcag gttttgggaa ccatctgcag aaaagatgtt taatttttgc gtattgattc
    48781 gtagacgaat acgattgcct ttttgtagtt cttcttcaaa agttgattct tcactaactt
    48841 gaatttttga ggcaaaacct gtctcaatga aatactccgt atttagtcca agatagacgc
    48901 tatcgctaat agttccttca atatctccag attcatcttt gataaactct tcgggacgaa
    48961 tgcttacatg aatagcttgc tcctcaacct gatcaagagc tggcattcga agggcatagc
    49021 catctgaaaa gacgatataa gcgccgtcgc tccgtttttc aagattggca gggataatat
    49081 ttgtgcgtcc gataaaggtt gccacaaact cattagctgg tttatgatag agttcttttg
    49141 gtcggccgat ttgttggatc accccatctt tcataacagc aatttgatct gaaatagcca
    49201 tggcttcttc ttggtcgtgg gttacataaa cagttgtaat tcccacttcg tgttggattt
    49261 ctcggatggc ttgacgcata tccaagcgaa gtttggcctc cagattacta agtggctcgt
    49321 ccatgaggag aacacttgga ttaaccgcta aggcgcatgc caaggtgaca cgttgttgtt
    49381 gtccaccact gagtttatcg ggctttcgat ccgcatattg agcaatttgc atgagttcaa
    49441 gatacttgtt ggtctgttga atcaattctt cttttggaac cttcttttgc ttaagaccaa
    49501 aagcaacgtt gtctcggaca gtcaaatgtg ggaaaatagc gtagttttgg aaaaccatcc
    49561 cgatattgcg tttgctgggt tccatattat tgatttttgt atcatcgaag taaaattctc
    49621 catctttgat actgttgaaa cctgcaatca tacgaagaag ggtcgttttc ccacatcctg
    49681 aaggtccaag aagggtaaag agacttcctt ttggaattgt aatgttcaaa ttctcaataa
    49741 cagggacatc gtggtagatt ttttggcgtt aataattttg atctcactca tagtgaacct
    49801 tttttactgt ttagattgga tatctgtaaa gacttcgttg tatttcttaa cgatatctga
    49861 tttattcttg atgacataat cataatcttc agtgagtgtt ttgattttgt caattggttt
    49921 catgttttcg cttgttttag catttttacg aacaggacgg ttagtagtgg ttgtaccaag
    49981 tgtatcttgt acttcttgag agataataaa atcgataaat ttcttgtcat tttccatatt
    50041 tttagctttt ttaacgatag cagcactagc aggtaggaag acggttcctt cttttggata
    50101 gactacttta atgttagctc cgtcatttaa gagtttaact gctggatcct cataagagag
    50161 accaacagcc atttctccat caacgactgc tttatagaca ccagatgaac ttgaaccgat
    50221 tttatagtgt attgaaacta gaatagtaca cctctacttc taaaacattg ttagaaatcg
    50281 atttgactgt cctgattaat ttgtcctgtt cttatttcat ttcactatgc catcaataag
    50341 tgtgaaaaga tcttttacat aagaccaagc cttatcatct ttgtaaccac cttgagcttg
    50401 tagcatattt gttaattgag caaaggcgct agaagagttt gctgggtcag cagttgcgat
    50461 tttttctttt agttcaggtt tgagaagatc gttatatcct tcgatgttca tgcctttagt
    50521 taaatcaggg tggacgatta aaacactacc atctagtgta taaggagtag agaagccagt
    50581 tgtgttttga tattctttga taacattatc attttctttt gaagtatagt tttcaaagag
    50641 ttctccgtgg gtagtatatt gtgtataaga accaccaaag ataacatcag ctacaggaac
    50701 ttctttttct gactctagtt ttttgaaaag ttctccagta ccagcttgaa tcagttctac
    50761 tttgatacca tatttttctt caaaggcagg aatagttgct ccaattaagc cctctgagtt
    50821 tggtgaataa acgactagcg aaccgccgtc tcctttatca gatgaactgt catcggcaga
    50881 ttcattagaa gaacaagcag cataatacat ccatttcttt ttcatgatgg atacctccgt
    50941 tgtgttattt aagtttattt taaaacaatg taagcgtttt taaaacatac aattctattc
    51001 tatagtgtat tgaatctata acagtacact ttgactgcta aaatatttct ataaattaat
    51061 ttgactttcc tgatagagat gttcacatct tatttcaatt cactatatta gagtaaaatt
    51121 ctctacaaaa agaagaatag cctattttac tattcttctg agtgatttca attcctttgg
    51181 ggaaatatgg agatactttt taaatcctga caaatggttg tttctttttc taaatcggtg
    51241 atactgtatc ggagaatgcg cgtgaggtca caaaggctgc gatagagctt ctatggagaa
    51301 tttctttttg gagagatttt ttaaaggaat gagacatccg ctacctcctt ggaaggtttt
    51361 tttgtgataa gtatagcagt ttaaaaattc tagagcaagg taaaatagaa aaggtttggc
    51421 taaaattttt tagctaaacc ttttgaaaag ttaagtatgt attatagatg cttgttatct
    51481 gcaaatagct agagcatttc aaggaaataa actagaaata acaattaata tggtggttca
    51541 gaaatacctt ttgataatca ataagatctt caggatgaag tgcgttgatt ccatccattt
    51601 catatttccg atttaataaa cgatatttgt tttcaccaaa ttgatgaaaa gggagtagtt
    51661 gaacttggtc gatatttaat gagttaaata gagtagcgaa tttttctgca tcctctaaac
    51721 tattgttaaa attaggaata actgggattc ttaaaacgat agttttattt tgtgaaaaag
    51781 cataatgaat gtttttaata atcatttgat taaaaacccc agtcactttt ttatgtttta
    51841 tagaattata atgttttagg tctgtgtaga taaaatccac atattgaatt aaatcaataa
    51901 atttttcatg atcaacaaag gcagtagttt caatggcagt gtgtatgtga tgttctttag
    51961 ctgattttaa gatggcttta gcaaattcaa actgagcaaa tatttcacct cctgataaag
    52021 ttaaacctcc accggattct tcgtaaaatt ctttgtcttt taatacctct gtaataattt
    52081 cttctacagt cttttcttca ccgactaagg tgaatttctc tcgttgagca tctttcattt
    52141 tttcaggttt cattctttga gattcaggat tagaacacca tggacagcgc agaggacatc
    52201 cttttaaaaa aacagttgta cgaatacccg ggccgtcatg aattgaaaag tgttgaatat
    52261 taaaaataat tcctttagat atttccataa gagttcctcc tcttcataag tctatcctat
    52321 cacaaaaacg aaagaaaaac aattacgaac gaaaggttaa tttattgttt tttgcttttt
    52381 attttggtag aataaaacgg aggtgtaaaa tgaaaagatt ggaacaaatt attaaattag
    52441 tatcagaata tgaaaagatc gacgttaata cattatcgga aaaattaaat gtatcgaaag
    52501 taacgattag aaaagattta gataaattag agtcaaaagg tttattacac agagagcatg
    52561 gatatgctgt attaaatagt ggagatgact taaatgtacg tttgtcaatt aattatgaaa
    52621 ttaagagaaa aattgttcag gaagcagtaa aattggtgtc agataatgaa acaataatga
    52681 tagaatctgg atcgacctgt gctttacttg ctgaggaaat ttgcaagcaa aaaagaaatg
    52741 ttacgattgt aacaaattca ttttttatag caaattttgt gagagcttat gattcatgtc
    52801 gtattattgt tcttggtgga gagtttcaga aggattcaca ggttactgtg ggacctttat
    52861 taaaagaaat gatacagact tttcatgtgc atcaagcttt tgttgggaca gatggttacg
    52921 ataaagagat gggctttaca ggaaaagatt taatgcgcag tgaggtagtt caatatattt
    52981 cagcagcgtc ggataaagtc attgtactaa ctgactcaag taaatttgat aaaaggggta
    53041 cagtaagaag atttgcttta agtcaagtct atgaagtaat aacagacgaa aaactttcta
    53101 aacaaaatat agctacatta gaaaatgctg ggataatggt taaggtagtt tcgtaagagg
    53161 ttaagtgtat gaatcaagat aggaataaac tgctctctaa aattgcttat ctgtattata
    53221 ttgaaaactt aaatcagtca caaatagcag caaaattagg aatttataga acctctatta
    53281 gtagaatgtt aacagaagca aggaatgcag gaattgttaa aattgaaata gagaattttg
    53341 ataccaatat gtttaagttg gaaaattatg taaaagaaaa atatggtctg gaaagtttag
    53401 aaattattcc aaatgaattt gatgataatc caacaatttt atctgaaaga atttctcaag
    53461 ttgcagcagg tgtccttagg aatctaattg atgataatat gaaaattggc ttttcttggg
    53521 ggaaaagttt aagtaattta gtagatttaa ttcacagtaa aagtgtccga aatgttcact
    53581 tctatcctct agcaggtggt cctagtcaca tacacgctaa ataccatgtg aatacactga
    53641 tttatgaaat gtctagaaaa tttcatggag agtgtacatt tatgaatgca acgattgtgc
    53701 aagaaaataa attgttagca gatggtattt tgcaatcaag atattttgaa aatttgaaaa
    53761 atagttggaa agatttagat atagctgtag tcggaattgg tgattttagc aataaaggaa
    53821 agcatcaatg gttagacatg cttacagagg atgactttaa agaattgacc aaagttaaaa
    53881 ctgtaggaga aatttgttgt cgattttttg attcaaaagg taaagaagtc tatgaaaact
    53941 tacaggaaag aacgatagca atctctttgg aagatttaaa aaatattcct caaagtttag
    54001 ctgttgctta cggtgatacg aaagtatctt cgattctttc tgtcttgcgt gctaatttag
    54061 taaatcattt gattacagac aaaaatacaa ttttaaaagt tttggaagaa gatggggatt
    54121 tgacttttag agagattcta ggtgagtgaa aatgatagac tgattcagtt tatcattttt
    54181 ctttttagtt gattgcacat ttgtgcttat ataaacaaaa atagtttatc tgttgttttt
    54241 ggattgacaa ctttattatg tagttgtatt ctatagttac aaaagaaaat tttaaaattt
    54301 caaatgaaaa aagcttttta catagtgaaa tgaggaggaa tttatggaaa ttattgttcc
    54361 agatcaaatt atcatgggtt taattttata tgctggcgat gcgaaacaac atatttataa
    54421 agcattagat tacataaaga acggtacatg tgaacgatgt gaagaagaaa tccagttagc
    54481 tgatgcagcc ttattagaag ctcataatct acaaacaaaa ttcttggcac aggaagcgtc
    54541 tggtacaaag acagaaatta cagctctctt tgttcattca caagatcatc tcatgactag
    54601 tatgacggag attaatttaa tcaaagaaat tattagtttg agaaaagaac ttcataaaaa
    54661 ataatactag agtattatca ttgttattaa catagaggag gaaaacataa tggtgaagat
    54721 tggtttgttt tgtgcagcag gtttttctac tggtatgctt gtaaataata tgaaaattgc
    54781 agcgcaatct agtggagttg aggcagaaat agaggcgttt tctcagtcta aattagcgga
    54841 ttatgcgcca aatatagatg ttgcactatt gggtccacaa gttgcttata cattagataa
    54901 atcaaaagaa atttgtgata agtgtgatgt tccgatagct gttattccga tgatggacta
    54961 tggtatgtta gatgggaaaa aagtattaga tttggcccta tctttgatta gtgggtaaga
    55021 aaaggagatt tattatgtca aagatggatg ttcagaaaat cattgcaccg atgatgaagt
    55081 ttgtgaatat gcgtggcatt atagctctaa aagatgggat gttagcaatt ttgccattga
    55141 cagtagttgg tagtttgttc ttgattatgg gacaattgcc gttcgaagga ttaaataaga
    55201 gcattgctag tgtttttgga gctaattgga cagagccgtt tatgcaagta tattcaggaa
    55261 cttttgctat tatgggtcta atttcttgtt tttcaattgc ctattcttat gctaagaata
    55321 gcggagtaga ggctttacca gctggagttc tatctgtatc tgcattcttt attttgctaa
    55381 gatcatctta tatccctaaa caaggtgagg cgattgggga cgctattagt aaagtttggt
    55441 ttggaggcca aggaattatc ggtgctatca ttataggttt ggtagtagga agtatttata
    55501 ccttctttat aaagagaaaa attgttatta agatgccaga acaagttcca caagctattg
    55561 cgaaacagtt tgaagcaatg attccagcat ttgtaatttt cttatcttct atgattgtat
    55621 atattttagc gaagtcattg actaatggcg gaacattcat agaaatgatt tattctgcta
    55681 ttcaagttcc gttgcaaggt ttaactggat ctttgtatgg tgctattgga attgcattct
    55741 ttatatcatt tttgtggtgg tttggtgttc atgggcaatc ggtagtaaat ggagtagtga
    55801 cagctctgct tttatctaat cttgatgcta ataaagctat gttagcctct ggtaatctat
    55861 cattagaaaa tggtgcacat attgttactc aacaattttt agattcattt ttaattctat
    55921 caggttcagg gattacgttt ggtcttgtag ttgccatgct ttttgcagca aaatcaaaac
    55981 aataccaagc cttaggaaaa gttgcagctt ttccagcaat atttaacgta aatgagccag
    56041 ttgtatttgg atttccgatt gtcatgaatc cagttatgtt tgtacctttc attcttgttc
    56101 ctgtacttgc agctgtgata gtatatggag ctattgcaac aggtttcatg cagccattct
    56161 caggggtaac attgccttgg agtacaccag ctattctatc aggatttttg gtgggtggat
    56221 ggcaaggtgt tgtgactcag ttattagtat tggtgatatc tacagtggtt tactttccat
    56281 tctttaagat acaagatcgt ttagcttacc aaaatgaaat caaacaatct tagaggtatt
    56341 tgtgtgttac tgttaaactc acacatttgt gctaaaaatt agagagttaa aatttttcta
    56401 gttaaaagct tgaaaatttc tataaaaatc ggtattatat tttcgaaaga aataaaaata
    56461 ttttcgaaag aaaggtgctt acgatggtaa atacagaagt agcaagaaca acaatcaaga
    56521 cagaatattt tggcagcctt actgaaagga tgaacaaata tcgagaagat gttttaaata
    56581 aaaaacctta tattgatgct gagagagcag ttctagcaac acgcgcctat gaacgataca
    56641 aggaacaacc taatgtccta aaacgtgcat atatgctgaa agaaattttg gaaaatatgt
    56701 ctatctatat tgaagaagaa tctatgattg cgggaaatca agcttcttcc aataaagatg
    56761 ctcctatttt tccggaatat acgctagaat ttgttctcaa tgagttggat ctttttgaaa
    56821 agcgtgatgg agatgttttc tatattacag aagaaacaaa agaacaactt agaagtattg
    56881 ctccgttttg ggaaaataat aatttacgtg ctagagctgg tgccttatta cctgaagaag
    56941 tgtctgttta tatggaaaca ggattcttcg gtatggaagg taagatgaat tctggagatg
    57001 ctcacttagc agttaactat cagaaacttt tgcaatttgg tttaagaggt tttgaagagc
    57061 gggctcgtaa agcaaaagta gctctagatt taacagatcc agcaagtatt gataaatatc
    57121 atttttacga ctctatattt atcgtaatcg atgctattaa agtatatgca aagcgctttg
    57181 ttgctcttgc taaaagttta gccgaaaatg caaatcctaa acgtaagaaa gaattacttg
    57241 agattgcaga tatttgctct agagtcccat atgaaccggc aactactttt gcagaagcta
    57301 ttcaatcagt ttggtttatt caatgtattt tacaaattga atctaatggc cactctcttt
    57361 catatggccg ttttgatcaa tatatgtatc catatatgaa ggctgattta gaaagtggta
    57421 aagaaacaga agatagcatt gttgaacgtc tgacaaatct ttggattaag acaattacaa
    57481 ttaataaggt tcgcagtcaa tcacatacat tttcttcagc aggaagtcct ttatatcaaa
    57541 atgttacaat tggtggacag actcgagata agaaggatgc tgttaaccca ttatcttatt
    57601 tggtattaaa atcagttgca caaacccatc taccgcaacc taatctaact gtacgttacc
    57661 atgcaggttt agatgctcgt ttcatgaatg agtgtattga agtgatgaaa cttggttttg
    57721 gtatgcctgc atttaataat gatgagatta ttattccttc ttttattgca aaaggagtat
    57781 tggaagatga tgcttatgat tacagtgcca ttggatgtgt tgaaacggca gttccaggga
    57841 aatggggcta tcgttgcaca ggtatgagtt atatgaactt ccctaaggtt ctacttatca
    57901 cgatgaatga tggaattgat ccggcttcgg gtaaacggtt tgcaccaagc tttggtcatt
    57961 ttaaggatat gaagaacttt tctgaattag aaaatgcttg ggataaaaca ctaagatatt
    58021 tgacacgaat gagtgttatt gttgaaaatt ctattgattt atcattggaa cgagaagttc
    58081 ctgatattct atgttcagca ttgactgatg attgtattgg tcgtggaaaa caccttaaag
    58141 aaggtggagc agtatatgat tatatatcag gattgcaagt tggaattgca aatttgtcgg
    58201 attcgttagc tgcaattaaa aaattggtgt ttgaggaaga acgtataagc ccaagtcaac
    58261 tttggcatgc actggaaaca gattatgccg gagaagaagg taaggtcatt caagaaatgt
    58321 tgattcatga tgcacctaag tatggtaatg atgatgatta tgctgacaaa ttggttactg
    58381 ctgcttatga catttatgtt gatgaaattg ctaaatatcc taatacacgt tatggaagag
    58441 ggcctattgg aggaattcgt tattcaggaa catcttctat atcagccaac gtagggcagg
    58501 gacgtggaac attagcaact ccagatggac gcaacgcggg tacaccgtta gcagagggtt
    58561 gttcaccatc acataatatg gatcaacacg gccctacatc tgttttaaaa tctgtttcaa
    58621 aattaccaac agatgaaatc gtaggtgggg ttctcttaaa tcagaaagta aatcctcaaa
    58681 cgttagccaa agaagaagat aaattaaaac taattgcttt gttacgaaca ttctttaatc
    58741 gtttacatgg gtaccatatt caatacaatg ttgtttccag agagacgttg attgacgctc
    58801 agaaacatcc tgaaaaacac agagacttaa ttgttcgtgt tgcaggatac tctgcattct
    58861 tcaatgttct ttctaaggca acccaagatg acattatagg acgtactgag catactttgt
    58921 aaaataaaga ggttcttttt atggaattta tgcttgacac attaaattta gatgagatta
    58981 aaaagtggtc tgaaattttg ccgctagctg gggtaacttc aaatcccact attgcaaaaa
    59041 gagagggttc tattaatttt tttgaacgaa tcaaagatgt aagagaattg attggctcta
    59101 caccctctat tcatgttcag gtgatttctc aagattttga aggcatctta aaggatgctc
    59161 atgaaattcg aagacaagca ggagatgata tatttatcaa agtacctgtt actccagctg
    59221 gattacgtgc aataaaggtg ctaaaaaaag agggctacca tatcactgca acagctattt
    59281 atacagttat tcagggatta ttagctatcg aagtaggagc ggattaccta gctccatatt
    59341 ataatagaat ggaaaatctg aacattgatt caaattctgt cattcgtcaa ttagctcttg
    59401 ctattgatag acagaactct cctagtaaga ttttagctgc atcctttaaa aatgtagcac
    59461 aagtaaataa tgctttagct gcaggtgcgc atgctgttac agcaggagcg gatgtttttg
    59521 aatcagcttt cgccatgcca tctatccaaa aggcggttga tgatttttct gacgattggt
    59581 ttgttattca aaatagtcgt tccatttaga tagagaggaa atacatatga gaatttttgc
    59641 tagtccttct agatatattc agggggaaaa tgccttgttt gaaaatgcca aatcaatttt
    59701 ggatttggga aattgcccta ttctattatg cgatcagttg gtttatgata ttgttggaaa
    59761 acgatttgaa gattacctac ataggtatgg tttccatatt gttctggcgc tatttaatgg
    59821 tgaagcttct gacaatgaaa tcaatcgagt tgttgccttg gctgagaaag aaaattgtga
    59881 tagtattatc ggtcttggtg ggggaaagac gattgatagc gcaaaagcta ttgcagattt
    59941 gattgaaaag cctgttatta ttgctccaac aattgcatcg accgacgcac ctgtatctgc
    60001 tttatctgtt atttatacag atgaaggtgc atttgatcat tatctatttt attctaaaaa
    60061 tccagattta gttttggttg atacaaaagt tatttcacaa gcccctaagc gtttattagc
    60121 gtctggtatt gcagatggtt tagcaacttg ggttgaggcg cgtgcggtta tgcaggcaaa
    60181 tggaaaaact atgttgggac aacagcaaac attggctgga gttgcaattg cgaagaaatg
    60241 tgaagaaacg ctgtttgcag atggtttaca ggctatggca gcttgtgaag ctaaagtggt
    60301 gacaccagca ttagaaaata ttgttgaagc taatacttta ttgagtggtc taggttttga
    60361 aagtggagga ttagctgcgg cgcatgcaat tcataatggt tttactgcat tgacaggtga
    60421 cattcatcat ttaacacatg gtgaaaaagt agcttatgga actttagtac aactattatt
    60481 ggaaaataga cctaaagaag aacttgataa gtatattgag ttttacaaaa aaattggtat
    60541 gccaacaact ctaaaagaaa tgcatttgga tcaagttgga tatgatgatt taataaaagt
    60601 tggtaaacaa gcaactatgg agggtgagac aattcatcag atgccgttta agatttcgcc
    60661 ttcagatgtt gctcaagcta ttatcgctgt agatgcctat gtaaattcaa aataaacaat
    60721 aaggactact gttttccaaa tggtagtctt ttattgatcc ctgtattgaa ttctatagaa
    60781 gattgaaata ggatgagaac aaatcgattg ggaaagtaaa attaatttct ataaatgttt
    60841 tagcaattgt ttcgtactat ttcagattca gtctactata tgttcttcat aaatcaaaaa
    60901 gcgacatagg ttgtcggcta tttattgtga atacattaat tagcattcca gttttatctt
    60961 cggtctaaaa taagtatttt gtgctatacg agataagctt cttgacttac tccttgattt
    61021 actgcataac aatgggataa aaagtgggag atagagcaat tcatagtcat caaaattaat
    61081 gagatacagt atacagtttt tcctttaaac acatttcaaa ttccctcaaa aatggtataa
    61141 tagtaacatc acaaaattgg agagagacca tgagttttta caatcataaa gaaattgagc
    61201 ctaagtggca gggctactgg gcagaacatc atacatttaa gacaggaaca gatgcatcaa
    61261 aacctaagtt ttatgcgctt gatatgttcc cttatccgtc tggagctggt ctgcacgtag
    61321 gacacccaga aggttatact gcaaccgata tcctcagtcg ttacaaacgt gcgcaaggct
    61381 acaatgtcct tcacccaatg ggttgggatg cttttggttt gcctgcagag caatacgcta
    61441 tggatactgg taatgaccca gcagaattta cagcggaaaa cattgccaac ttcaaacgtc
    61501 aaattaatgc gcttggattt tcttatgact gggatcgtga agtcaacaca acagatccaa
    61561 actactacaa gtggactcaa tggattttca ccaagcttta cgaaaaaggc ttggcctatg
    61621 aagctgaagt gccagtaaac tgggttgagg aattgggaac tgccattgcc aatgaagaag
    61681 tgcttcctga cggaacttct gagcgtggag gctatccagt tgtccgcaaa ccaatgcgcc
    61741 aatggatgct caaaatcacg gcttacgcag agcgcttgct caatgactta gatgaactag
    61801 attggtcaga gtctatcaag gatatgcaac gcaactggat tggtaaatca actggtgcca
    61861 atgtaacttt caaagtaaaa ggaacagaca aggaatttac agtctttact actcgtccgg
    61921 acacactttt cggtgcgact ttcactgtct tggctcctga acatgaatta gtagacgcta
    61981 tcacaagttc agagcaagca gaagctgtag cagactataa acaccaagcc agccttaagt
    62041 ctgacttggc tcgtacagac cttgctaaag aaaaaacagg ggtttggact ggtgcttatg
    62101 ccatcaaccc tgtcaatggt aaggaaatgc caatctggat tgcagactat gtccttgcta
    62161 gttatggaac aggtgcggtt atggctgtgc ctgcccacga ccaacgtgac tgggaatttg
    62221 ccaaacaatt tgaccttcca atcgtcgaag tacttgaagg tggaaatgtc gaagaagctg
    62281 cctacacaga ggatggcctg catgtcaatt cagacttcct agatggactc aacaaagaag
    62341 acgctattgc caagattgtg gcttggttgg aagaaaaagg ctgtggtcag gagaaggtta
    62401 cctaccgtct ccgcgactgg ctctttagcc gtcaacgtta ctggggtgag ccaattccaa
    62461 tcattcattg ggaagatgga acttcaacag ctgttcctga aactgaattg ccgcttgtct
    62521 tgcctgtaac caaggatatc cgtccttcag gtactggtga aagtccacta gctaacttga
    62581 cagattggct tgaagtgact cgtgaagatg gtgtcaaagg tcgtcgtgaa accaacacta
    62641 tgccacaatg ggctggttca agctggtact acctccgcta tattgatcca cacaatactg
    62701 agaagttggc tgatgaggac ctcctcaaac aatggttgcc agtagatatc tacgtgggtg
    62761 gtgcagagca tgctgttctt cacttgcttt acgctcgttt ctggcacaaa ttcctctatg
    62821 acctcgatgt tgttccgact aaggaaccat tccaaaaact ctttaaccaa gggatgattt
    62881 tgggaacaag ctaccgtgac caccgtggtg ctcttgtggc aaccgacaag gttgaaaaac
    62941 gtgatggttc cttcttccat gtagaaacag gggaagagtt ggagcaagcg ccagccaaga
    63001 tgtctaaatc gctcaagaac gttgttaacc cagacgatgt ggtggaacaa tacggtgccg
    63061 atacccttcg tgtttatgaa atgtttatgg gaccactcga tgcttcgatt gcttggtcag
    63121 aagaaggttt ggaaggaagc cgtaagttcc ttgaccgagt ttaccgtttg attacaagta
    63181 aagaaatcct tgcggaaaac aatggtgctc ttgacaaggt ttacaacgaa acagtcaaag
    63241 ctgttactga gcaaattgag tctctcaaat tcaacacagc tattgcccaa cttatggtct
    63301 ttgtcaatgc tgctaacaag gaagataagc tttatgttga ctatgccaaa ggctttattc
    63361 aattgattgc accatttgca cctcacttgg cagaagaact ctggcaaaca gtcgcagaaa
    63421 caggtgagtc aatctcttat gtagcttggc caacttggga cgaaagcaaa ttggttgaag
    63481 atgaaattga aattgtcgtc caaatcaaag gaaaagttcg tgccaaactc atggttgcta
    63541 aagatctatc acgtgaagaa ttacaagaaa tcgctttagc tgatgaaaaa gtcaaagcag
    63601 aaattgacgg taaggaaatc gtgaaagtaa ttgcggtacc gaataaactc gttaatatcg
    63661 tcgttaaata acgagtttat tagctctatc tgccaccttc aatagtccac tggactattg
    63721 aagccaacta aattagttaa cattgttgtg aaataaaaag aagtaactct agagtatgaa
    63781 ctttagagtt acttttagaa taggtcagaa tgaatagatt agtgaacgat agcaggaagt
    63841 gtgaaatttt attatttaag atttctatta ttgtagtgtc agttatagta ttagtaatgt
    63901 caatagtggg tgcatattat tttaaaaatt gtctttggga caaggaaagt ttaaatttga
    63961 cactcacttt agacactctc tttgcagttt ttattacttt ctttagtttt tcaaatgata
    64021 aatcaagcaa tatttttgga atatctccaa atgaaattat aaggacacag tctgaagtgt
    64081 atcaattttt atttacaaaa tcttttgcaa ctctattaat agtgacaaat ttgttatcca
    64141 ttattcatta ttttggagcc aaaaatttaa tagtttcttt taatgaaact tatcctcgta
    64201 ttcaatccag tgttggtatc atgtggctac cactgtttgg tattctgata atctatttta
    64261 tttggctctt aatagaggg
//
LOCUS       DBERDK010000003        54776 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.003, whole genome shotgun sequence.
ACCESSION   DBERDK010000003 DBERDK010000000
VERSION     DBERDK010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 54776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54776
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.003"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(11..562)
                     /locus_tag="ACL73L_000156"
     CDS             complement(11..562)
                     /locus_tag="ACL73L_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771210.1"
                     /translation="MPANTKVIFQEMFADFQNYYVLIGGTATSIVLDSQGFKSRTTKD
                     YDMVIIDEVKNKEFYTTLNHFLELGEYQGSQKDEKAQLFRFTTTNPEFHSMIELFSIL
                     PEYPLKKDGREIPLYFDQDASLSALLLDEDYYNILVHEKETIQGYSVLSNCGLYSSKI
                     SSNHVSFHLQPQNSVLSSLQLAS"
     gene            complement(552..749)
                     /locus_tag="ACL73L_000157"
     CDS             complement(552..749)
                     /locus_tag="ACL73L_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771211.1"
                     /translation="MLEIWKYRPFVSEFWNDFKNNHDKQFVDPISLYLTLKDDDDPRI
                     EEESEALENMILQYLGEDDAS"
     gene            complement(753..1547)
                     /locus_tag="ACL73L_000158"
     CDS             complement(753..1547)
                     /locus_tag="ACL73L_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771212.1"
                     /translation="MNVKKIMSIFQSFYVDVSIEELTLTLPISFVKRFEYTQMTFHKE
                     SFLLIKEKRRGSLSSFVTQARTMGEKANMDVVLVFSKLSDSEKKQLLQARVPFVDFKG
                     NLFFPPLGLVLNANDTEIPKELTPSEQLTWIAFLLTKGQKVVNVDLLSQVTGLPNSTI
                     YRCLRTFKALYWLNKQNKLYTYTVSKKELFLKSVSCLFNPIKKRILLPDGDIKQIKSV
                     SNLLYGGAYALSHSTFLAETDENTSYVIWQRKFNQLSLPLSQHVLK"
     gene            complement(1955..2380)
                     /locus_tag="ACL73L_000159"
     CDS             complement(1955..2380)
                     /locus_tag="ACL73L_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771213.1"
                     /translation="MTSRVFIDADCISVFLWVGTEHLLEKLYLGKIVIPQEVYDEINI
                     PTIPHLKSRIDQLVTKGSAEIVSIDIGTEEYALYRDLTRNHDSNKIIGKGEATSIFLA
                     KKHNGILGSNNLRDVKPYVEEFSLEHMTTGDILIEAFKA"
     gene            complement(2509..2868)
                     /locus_tag="ACL73L_000160"
     CDS             complement(2509..2868)
                     /locus_tag="ACL73L_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HGS0771214.1"
                     /translation="MKKSSVSLILIGEGDETERKADQFASYFLIFPSSLYRMVEEIRE
                     NANRTHLEVEDIIKLGQFYGISHKAMLYRLRNDGYLDAEEIKNMDISVIETASRLGYD
                     TSLYRPLSESKKEMALG"
     gene            complement(2914..3120)
                     /locus_tag="ACL73L_000161"
     CDS             complement(2914..3120)
                     /locus_tag="ACL73L_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771215.1"
                     /translation="MDLSNKASNLRKKLGADGESPIDIFKLVQKIENLTLVFYGLGKN
                     ISGVCYKGTQFSLIAVNSDMPLGR"
     gene            complement(3107..3268)
                     /locus_tag="ACL73L_000162"
     CDS             complement(3107..3268)
                     /locus_tag="ACL73L_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGS0771216.1"
                     /translation="MYVINVCDVGKRIKELRISSNLTQDKIAEYLSLNQSMIAKMEKG
                     ERNITNGFK"
     gene            complement(3640..4596)
                     /gene="lytA"
                     /locus_tag="ACL73L_000163"
     CDS             complement(3640..4596)
                     /gene="lytA"
                     /locus_tag="ACL73L_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HGS0771217.1"
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKHDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIADKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPEFTVEPDGLITVK"
     gene            complement(4972..6342)
                     /locus_tag="ACL73L_000164"
     CDS             complement(4972..6342)
                     /locus_tag="ACL73L_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HGS0771218.1"
                     /translation="MNKKRTVDLIHGPILPSLLSFTFPILLSNIFQQLYNTADVLIVG
                     RFLGQESLAAVGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVAATWIL
                     GALLSILVMLLGFLGLYPLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLR
                     SIGDSLAALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIISQGLSAVLCFYYIRK
                     SVPELLPQFKHFKWDKSLYADLLEQGLAMGLMSSIVSIGSVILQFSVNTFGAVIISAQ
                     TAARRIMTFALLPMTAISASMTTFASQNLGAKRPDRIVQGLRIGSRLSISWAVFVCIF
                     LFLASPALVSFLASSTDGYLIENGSLYLQISSAFYPILSLLLIYRNCLQGLGQKILPL
                     VSSFIELIGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAIL
                     ATKVQS"
     gene            complement(6649..7815)
                     /gene="recA"
                     /locus_tag="ACL73L_000165"
     CDS             complement(6649..7815)
                     /gene="recA"
                     /locus_tag="ACL73L_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HGS0771219.1"
                     /translation="MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAV
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVTLDLGDELEIEIEE"
     gene            complement(7870..9126)
                     /locus_tag="ACL73L_000166"
     CDS             complement(7870..9126)
                     /locus_tag="ACL73L_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HGS0771220.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FALRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNQLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQIDPTLAPYAKTGEVTLR
                     LSTKASSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVANFSGVSSIFKGGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQDQGTEVIKVNIG
                     GRSRADVRHIAVMHAFNLVRKALLSD"
     gene            complement(9211..10227)
                     /locus_tag="ACL73L_000167"
     CDS             complement(9211..10227)
                     /locus_tag="ACL73L_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HGS0771221.1"
                     /translation="MVKKIIGMVLALLSVTVVGVGVFAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNAERTETWVGRSDSMILMTVNPKTKKTTMMSLERDIL
                     TRIESGNGQAHEAKLNSAYADGGAELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVG
                     GITVNNILGFPISISDQEEFNTISIGVGEQHIGGEEALVYARMRYQDPEGDYGRQKRQ
                     REVIQKVMEKALSLNSVGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQ
                     QLQGEGEILQGVSYQIVSRAHMLEMQNILRRSLGQEEVTQLETNAVLFEDLFGRAPVG
                     DEDN"
     gene            complement(10235..10753)
                     /locus_tag="ACL73L_000168"
     CDS             complement(10235..10753)
                     /locus_tag="ACL73L_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HGS0771222.1"
                     /translation="MEYELLIREAEPKDAAELVAFLNRVSLETDFTSLDGDGILLTSE
                     EMEIFLNKQASSDNQITLLAFLNGKIAGIVNITADQRKRVRHIGDLFIVIGKRYWNNG
                     LGSLLLEEAIEWAQASGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGK
                     FIDVYLMGKLIG"
     gene            complement(10743..11186)
                     /gene="tsaE"
                     /locus_tag="ACL73L_000169"
     CDS             complement(10743..11186)
                     /gene="tsaE"
                     /locus_tag="ACL73L_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HGS0771223.1"
                     /translation="MYTKNEEELQALGERLGHLLAKNDVLILTGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWG
                     NLLGDALPDAYLELEILKEADGRRLNFQAKGLRAEKLLEELQYGV"
     gene            complement(11272..11892)
                     /locus_tag="ACL73L_000170"
     CDS             complement(11272..11892)
                     /locus_tag="ACL73L_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771224.1"
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTAFFIGSMVFV
                     SGICAGVNYLYTRKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYIGLGLFFAAYLVPNSVKKEVSFYGHIFRDLVLVIVTLIFF"
     gene            complement(12275..13138)
                     /locus_tag="ACL73L_000171"
     CDS             complement(12275..13138)
                     /locus_tag="ACL73L_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGS0771225.1"
                     /translation="MNTLAEKFRLKRKELRLSQQTLAEGICEQSQISKIERGHFIPSA
                     DLLFKLSQRLEVPLDYFFNEQIEIKSNLSNFKQLSARLLDDRNYDDLEYIYRIEIERS
                     TFLTLEDRTYLEWIKAIIDFYQYDSKCEAISSLENILLKVSSNTLIYLKALNTLSNFY
                     SLVGREQEYEANYSHLIELYQTKNLDHQEFLFGYIRVRYNYAHYLVSKEKYNEAIQEA
                     LETIELCKQRQTSYQLAPLLILVGNAGAKFLDREQVKNYYIEARELCKIYNNPLMLMK
                     IENYLKELDTV"
     gene            13327..13497
                     /locus_tag="ACL73L_000172"
     CDS             13327..13497
                     /locus_tag="ACL73L_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhrA family quorum-sensing system peptide"
                     /protein_id="HGS0771226.1"
                     /translation="MKKNRGIQKLAILVLLGVFMFSNTIPYQQFIQKNRQLEIRVQSQ
                     KKSNGLDVGKAD"
     gene            13812..14108
                     /locus_tag="ACL73L_000173"
     CDS             13812..14108
                     /locus_tag="ACL73L_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000911560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HGS0771227.1"
                     /translation="MLKFRKVVASSLAVLAFSLALPVLAVSHRGGEWTYGGHHDPNNW
                     GAFSNYYHGSQYHWAYVGSNGRNNQKTVYAGARSAAYAFVNTNFGEQVTFDAGW"
     gene            14211..16226
                     /locus_tag="ACL73L_000174"
     CDS             14211..16226
                     /locus_tag="ACL73L_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HGS0771228.1"
                     /translation="MKKLFILISNLLASLFFVWVFTIWTDTYVSHYYPNVVVRDSSPE
                     TTFQHVATRLEKLAEETDSFIAIQHQDPNSEGTTVFSYTTFGDGKLPDGLQEKNLEDA
                     QSSSVETNYFVFDGHLDIHLLREELSQLGLTNMHLTIPSKLSTLMAIFSNGFQLISLL
                     IFILTFVALTLISQISQLRSSGIRLISGEKRWSIFLRPVGEDLKGIAVGFSLAGVLAI
                     LMQKILSLPTQSLMTIGAGLLSYNLILLSISLFFAQLFAVGIKKIHLMQIIKGQVPVR
                     GIISLILIGQLLAIIIVTLGIGSSLKYSQAWQQHRIGQEVWSQERQLITLSISREGTS
                     PGFDEQAQRKLRTWYQLMDLAVSEQKAFLSRHQLIDRTLQNGMASSKNLTTSTEWHDY
                     SPNGNVLIVTPHYLERQNIPVDTTIKQKMNHLNVGEFVLLLPEHLRSEEEHYKSVFED
                     DLTSRMSSQDERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQ
                     SILFWIDAVQNYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLLDNIQRERWVM
                     LAGAVLGIATSILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLG
                     FVASVFLQVEIGVAFLVLLLFTGLSLLQLHVQMQKENKMSILVLKGG"
     gene            16228..16860
                     /locus_tag="ACL73L_000175"
     CDS             16228..16860
                     /locus_tag="ACL73L_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0771229.1"
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLM
                     NMIGKLEPYDGTIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLIG
                     QKLSRSEQRLRQKQALEQVGLAYLDLDKRIFELSGGESQRVALAKIILKNPPFILADE
                     PTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWEMADEVFTMDHLK"
     gene            17001..17279
                     /locus_tag="ACL73L_000176"
     CDS             17001..17279
                     /locus_tag="ACL73L_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComA"
                     /protein_id="HGS0771230.1"
                     /translation="MKKFDNYIIEKPCDSNSDKLQKILIIENLVDDILQFPLRINNSV
                     GEIFLLQPFQKKTIFIPCYFEEDIVKVKDDDKVEWNLLEFQKFRAFLA"
     gene            complement(17788..18201)
                     /gene="ndk"
                     /locus_tag="ACL73L_000177"
     CDS             complement(17788..18201)
                     /gene="ndk"
                     /locus_tag="ACL73L_000177"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HGS0771231.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIDQHYQDLVGQSFYLPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEESLPGTI
                     RGDFAKAAGENEVIQNVVHGSDSEESAKREIALWF"
     gene            complement(18312..21989)
                     /gene="rpoC"
                     /locus_tag="ACL73L_000178"
     CDS             complement(18312..21989)
                     /gene="rpoC"
                     /locus_tag="ACL73L_000178"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HGS0771232.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYRGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMSYRNGYVHLHSRVGIATDSLNKPWTEEQRHKVLLTTVGKIL
                     FNDIMPEGLPYLQEPNNANLTEGVPAKYFLPLGGDIKEAISNLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYHHSTLAGLTVGIADIPVVDDKAEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIANQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGQVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPHAVNEEEYLNPPVEEEG
                     NEETTEVVVDTAVETVEETVE"
     gene            complement(22022..25633)
                     /gene="rpoB"
                     /locus_tag="ACL73L_000179"
     CDS             complement(22022..25633)
                     /gene="rpoB"
                     /locus_tag="ACL73L_000179"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HGS0771233.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHG
                     LKEVFEDVLPISNFIDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVVAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSRLNEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEEAEKATKAEATEEAAEQE"
     gene            26049..26119
                     /locus_tag="ACL73L_000180"
     tRNA            26049..26119
                     /locus_tag="ACL73L_000180"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26081..26083,aa:Cys,seq:gca)
     gene            complement(26317..27648)
                     /locus_tag="ACL73L_000181"
     CDS             complement(26317..27648)
                     /locus_tag="ACL73L_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HGS0771234.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTNTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRTAPVIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVHQIGEDTYIVQGTMTLNDFNNYFNVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELVSQNKQFILTNDKVKNGRVTKVKIQITEVEIEEETE"
     gene            complement(27939..28763)
                     /gene="endA"
                     /locus_tag="ACL73L_000182"
     CDS             complement(27939..28763)
                     /gene="endA"
                     /locus_tag="ACL73L_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HGS0771235.1"
                     /translation="MNKKTRQTLIGLLVLLLLSAGSYYIKQNELGNQAAKTKMSQKKQ
                     ASEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            complement(28802..28990)
                     /locus_tag="ACL73L_000183"
     CDS             complement(28802..28990)
                     /locus_tag="ACL73L_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HGS0771236.1"
                     /translation="MNKKSGYVIKRLLLVILVLILGLLALGIGLMVGYGILGKGQDPW
                     AILSPAKWQELIHKFTGN"
     gene            complement(28983..30266)
                     /gene="murA"
                     /locus_tag="ACL73L_000184"
     CDS             complement(28983..30266)
                     /gene="murA"
                     /locus_tag="ACL73L_000184"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HGS0771237.1"
                     /translation="MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVL
                     GPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHI
                     YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETIT
                     ITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVE
                     VIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFEN
                     RFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETV
                     VGKLVHLDRGYYGFHEKLAQLGAKIQRIEASDEDE"
     gene            complement(30290..30520)
                     /locus_tag="ACL73L_000185"
     CDS             complement(30290..30520)
                     /locus_tag="ACL73L_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HGS0771238.1"
                     /translation="MVQLLFTLSSHMFFIYVSFYLLKDLVRWEKVLKVTDDNTRKVRL
                     LVAFFSIVIGYILSSFFISLYHLWQEALRGLL"
     gene            complement(30578..31615)
                     /locus_tag="ACL73L_000186"
     CDS             complement(30578..31615)
                     /locus_tag="ACL73L_000186"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HGS0771239.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     VNDTEDKEAGAYQFVTVGVQHATLAHMIYAWLTPFTDIRSAQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKDIELKYFGVYVLNVTDNSTFKGILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIGLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHPDLRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGAAIFFAPDNPVSEEEQKAHPDAKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            complement(31599..32087)
                     /gene="coaD"
                     /locus_tag="ACL73L_000187"
     CDS             complement(31599..32087)
                     /gene="coaD"
                     /locus_tag="ACL73L_000187"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HGS0771240.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDIIERASRLFDKLCVGIFFNPHKQ
                     GFLPLENRKRGLEKALGHLENVEVVASHDELVVDVAKRLGATCLVRGLRNASDLQYEA
                     SFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGQDIACYVPESILEEIRNEK
                     KD"
     gene            complement(32077..32616)
                     /gene="rsmD"
                     /locus_tag="ACL73L_000188"
     CDS             complement(32077..32616)
                     /gene="rsmD"
                     /locus_tag="ACL73L_000188"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HGS0771241.1"
                     /translation="MKIVSGIYGGRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEGGR
                     VLDLYAGSGGLSIEAVSRGMSSAVLVERDRKAQTIVAENIQMTKEVGKFQLLKMDAER
                     ALEQVSGEFDLVFLDPPYAKEQIVADIEKMAERELFSEDVMVVCETDKAVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            complement(32681..33673)
                     /gene="asnA"
                     /locus_tag="ACL73L_000189"
     CDS             complement(32681..33673)
                     /gene="asnA"
                     /locus_tag="ACL73L_000189"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HGS0771242.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTPKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            complement(33784..34467)
                     /locus_tag="ACL73L_000190"
     CDS             complement(33784..34467)
                     /locus_tag="ACL73L_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HGS0771243.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYFLMQGRLWLTIATFAELALVFVASSMASRNSPAALPVFLLYSVLNGFTLSFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFLASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
     gene            complement(34523..35263)
                     /locus_tag="ACL73L_000191"
     CDS             complement(34523..35263)
                     /locus_tag="ACL73L_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HGS0771244.1"
                     /translation="MTIITSKANSVVKNTKKLHQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFTLENYRDQLATFSQTVWVSEDILLDLADSQTPQGIVAVVQREEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSQALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVAAGILIFHLS"
     gene            35297..35575
                     /locus_tag="ACL73L_000192"
     CDS             35297..35575
                     /locus_tag="ACL73L_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HGS0771245.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYSDFKIAN"
     gene            35653..36579
                     /gene="yidC"
                     /locus_tag="ACL73L_000193"
     CDS             35653..36579
                     /gene="yidC"
                     /locus_tag="ACL73L_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HGS0771246.1"
                     /translation="MKSIKRFAFSAMGVAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPILLQMPFFSAIYFAAQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPKLRKKVREELAKNPPKASAFSK
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(36654..37448)
                     /gene="pflA"
                     /locus_tag="ACL73L_000194"
     CDS             complement(36654..37448)
                     /gene="pflA"
                     /locus_tag="ACL73L_000194"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HGS0771247.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTRQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKKLMDTESYQDYMKRVHG"
     gene            complement(37574..38824)
                     /locus_tag="ACL73L_000195"
     CDS             complement(37574..38824)
                     /locus_tag="ACL73L_000195"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HGS0771248.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYARELGATINLDAFEDIEHLERVAGIPEIISCRYNPGGVFELGTDIMDNPGEAK
                     FGMTKDQLFEAFAILKEKGAKTFGIHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     IWLDFINLSGGIGVNYRPDQEPNDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFM
                     LAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHISNVTHPDGPAEAVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGK
                     ARQIRRAERPEDYFATLYGFDFEE"
     gene            complement(38891..39718)
                     /gene="purR"
                     /locus_tag="ACL73L_000196"
     CDS             complement(38891..39718)
                     /gene="purR"
                     /locus_tag="ACL73L_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HGS0771249.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLREFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK"
     gene            complement(39985..40926)
                     /locus_tag="ACL73L_000197"
     CDS             complement(39985..40926)
                     /locus_tag="ACL73L_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HGS0771250.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVAHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMTNKIFAMDNRSFYKPDLD"
     gene            complement(40916..42172)
                     /gene="rmuC"
                     /locus_tag="ACL73L_000198"
     CDS             complement(40916..42172)
                     /gene="rmuC"
                     /locus_tag="ACL73L_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HGS0771251.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVVVSDRLQEVRIELHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEAAYETGDKDEIERCRKSLLASVKRFARDIRNKYI
                     APPRTTNFGVLFVPTEGLYSEIVRNPVFFDDLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSADHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLHFQENEEEYED"
     gene            complement(42174..42836)
                     /locus_tag="ACL73L_000199"
     CDS             complement(42174..42836)
                     /locus_tag="ACL73L_000199"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HGS0771252.1"
                     /translation="MSEYKLSENNWTRVAVFAGGNRGHYRTDFDAFVGVDRGSLWVLE
                     EDLPLALAVGDFDSVTEEERQVIQKGAQYFVQARPEKDDTDLELALLTIFEQNPQAQV
                     TIFGALGGRIDHMLANVFLPSNPKLAPYMHQIEIEDGQNLITYCPEGISQLEPRSDYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKD
                     RR"
     gene            complement(42799..43455)
                     /gene="rpe"
                     /locus_tag="ACL73L_000200"
     CDS             complement(42799..43455)
                     /gene="rpe"
                     /locus_tag="ACL73L_000200"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HGS0771253.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDSHFVP
                     QISFGAGVVESLRPHSKMVFDCHLMVSNPEHHLEDFARAGADIISIHVEATPHIHGAL
                     QKIRSLGVKPSVVINPGTSVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALREEKGLNFEIEVDGGIDDQTIAQAKEAGATVFVAGSYVFKGEVNERVQTLRKQLD
                     "
     gene            complement(43466..44344)
                     /gene="rsgA"
                     /locus_tag="ACL73L_000201"
     CDS             complement(43466..44344)
                     /gene="rsgA"
                     /locus_tag="ACL73L_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HGS0771254.1"
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIVYISKMDLLEDRGELDFYQQTYGDIGYDFVTSKEELLSLLTGKVTVFMGQTGV
                     GKSTLLNKIAPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVS
                     RAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGVIATFRFDNYLQFLSEI
                     ENRRETYKKVSKKIPK"
     gene            complement(44346..45218)
                     /gene="rsmA"
                     /locus_tag="ACL73L_000202"
     CDS             complement(44346..45218)
                     /gene="rsmA"
                     /locus_tag="ACL73L_000202"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HGS0771255.1"
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DDQVNVIEIGPGIGALTEFLAERAAQVMAFEIDHRLVPILADTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPDLPIKVVANLPYYITTPILMHLIESGIPFCEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVED
                     ENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLA
                     EFAGLADALKGQGL"
     gene            complement(45349..45657)
                     /locus_tag="ACL73L_000203"
     CDS             complement(45349..45657)
                     /locus_tag="ACL73L_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771256.1"
                     /translation="MCEKIRIRRVSDYPSARGGLEDILIMENMTNHLLLVQIRVHGYL
                     LDFASIEGQRQKHYRLKNLPQMVELTVDDVEEDVDLTLPENRSYQEADFFERMFRENC
                     "
     gene            complement(45650..46291)
                     /locus_tag="ACL73L_000204"
     CDS             complement(45650..46291)
                     /locus_tag="ACL73L_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0771257.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLL
                     NILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFV
                     GQKISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILAD
                     EPTAALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            complement(46293..48323)
                     /locus_tag="ACL73L_000205"
     CDS             complement(46293..48323)
                     /locus_tag="ACL73L_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HGS0771258.1"
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMIN
                     QPVASREEVTEALSHLAVEHNSLIARRIVEPNEAGETRFTYATYGEGKLPEGLTISSK
                     ESAETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLTATPMVLL
                     SLAIFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQLICSVLVSSL
                     LGLGILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVYLLGLQENSLVDLLKGK
                     LPLKRIMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQSSDRYRLSFGW
                     SSAFADEEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYTP
                     SGNVIYVSPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSVYYQGLFTDYL
                     QNFSSESVEVTSQKHYLPQVRLAFTETGQERFLYNDGYKTTRQYLKDPIIVVLTPQAT
                     GTRPVAGMLWGTTANSALKLDRYGDSITALKEKGLYHKVSYLVKSQLFFAKVLNDKRV
                     EFYSLLIGTILTLSTAILLFDSMNLLYFEQFRRELMIKRLAGMTIYELHGKYLLAQGG
                     VLLLGLVLSSILTRDGLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            complement(48400..48696)
                     /locus_tag="ACL73L_000206"
     CDS             complement(48400..48696)
                     /locus_tag="ACL73L_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HGS0771259.1"
                     /translation="MKQTVKKLALVASIAATLGGGVSVASAAVQYPEGGVWTYGSGNG
                     GAYSNYYHPSKYHSSTVVSRKTGSSDKGYAGAGGTSRAWIRTSWGEKVAFYYNV"
     gene            complement(49016..49915)
                     /locus_tag="ACL73L_000207"
     CDS             complement(49016..49915)
                     /locus_tag="ACL73L_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGS0771260.1"
                     /translation="MLIGQKIKEIRIEKGISRPDFCGDEQELTVRQLSRIESGASQPS
                     LPKLAYIARRLGVPVYSLMPDFSALPSAYLELKYQILREPIYGKEEEYDKKEACLEEI
                     YKTYFDNLPKEEQLACEVLQACLDTSRTRRPEYAELILEEHMPQIIEKEAYSINDMLL
                     IRLFFYQMLIRKDLAKFINQIEKLMLFLLEQKKVTQIENYFIIRDTLISGMCCLEKVG
                     VTDCFNDYLSCLQEIMDKTQDYQKKPLVFMFLWKQALRVERDFSLAESFYQSSKTFAK
                     LIGDGFLVKKLTEEWQEDVKKYL"
     gene            complement(49987..50547)
                     /gene="rnmV"
                     /locus_tag="ACL73L_000208"
     CDS             complement(49987..50547)
                     /gene="rnmV"
                     /locus_tag="ACL73L_000208"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HGS0771261.1"
                     /translation="MKEKISQVIVVEGRDDTVNLKRYFDVETYETRGSAINDQDIERI
                     QRLHQRHGVIVFTDPDFNGERIRRMIMTAIPTVQHAFLKRDEAVPKSKTKGRSLGIEH
                     ASYEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            complement(50547..51320)
                     /locus_tag="ACL73L_000209"
     CDS             complement(50547..51320)
                     /locus_tag="ACL73L_000209"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HGS0771262.1"
                     /translation="MIFDTHTHLNVEEFAGREAEEIALAAEMGVIQMNIVGFDKPTIE
                     RALELVDEYEQLYATIGWHPTEAGTYTEEVEAYLLDKLKHPKVVALGEIGLDYHWMTA
                     PKEVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMTISFSGVVTFKKATDLQEAAKELPLDKILVETDAPYLAPVPKRGRENK
                     TAYTRYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            complement(51625..52518)
                     /locus_tag="ACL73L_000210"
     CDS             complement(51625..52518)
                     /locus_tag="ACL73L_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall surface anchor protein"
                     /protein_id="HGS0771263.1"
                     /translation="MVAQDPKAQDSTKPTAEKSTVKAPAQRVDVKDITHLTDEEKVKV
                     AILQANGSALDGATINVAGDGTATITFPDGSVVTILGKDTVQQSAKGESVTQEATPEY
                     KLENTPGGDKGGNTGSSDANANAGGGSQAGGSAHTGSQNSAQSQASKQLATEKESAKN
                     AIEKAAKDKQDEIKGAPLSDKEKAELLARVEAEKQAALKEIENAKTMEDVKEAETIGV
                     QAIAMVTVPKRLVAPNAAPKTTSAPQATAGTMQDVTYQSPAGKQLPNTGSASSAALAS
                     LGLVVATSGFALLGRKTRRRK"
     gene            complement(52802..52936)
                     /gene="rpmH"
                     /locus_tag="ACL73L_000211"
     CDS             complement(52802..52936)
                     /gene="rpmH"
                     /locus_tag="ACL73L_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HGS0771264.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA
                     "
     gene            complement(53081..54295)
                     /locus_tag="ACL73L_000212"
     CDS             complement(53081..54295)
                     /locus_tag="ACL73L_000212"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HGS0771265.1"
                     /translation="MKEYNKSSKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSDSKGIFSARKAIMQYCQLKKFPNVDIDDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKELLLEIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            complement(54451..54744)
                     /locus_tag="ACL73L_000213"
     CDS             complement(54451..54744)
                     /locus_tag="ACL73L_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771266.1"
                     /translation="MMKNSFQKSNFLYYGIILVSVLVEVIMIWQLESLVPLLYPSFIG
                     FLVFHVLYHLILFLVAKRSGRWDYLMIWGLFLMFNLLYDSFLGLLFLGLSFGM"
BASE COUNT        15919 a        12290 c        10130 g        16437 t
ORIGIN      
        1 aaagagcaaa ctaggaagct agctgcaggc tgctcaaaac actgttttga ggttgtagat
       61 ggaagctgac gtggtttgaa gagattttcg aagagtatag accacaatta ctcaataccg
      121 aatacccctg aatggtttct ttttcatgca ccaatatatt ataataatct tcatccaata
      181 ataaggctga taaactagca tcttggtcaa aatataaggg aatttctcga ccgtccttct
      241 ttaatggata ttctggtaaa atactaaata gttcaatcat agaatgaaac tcaggattag
      301 ttgttgtaaa tcgaaaaagc tgcgctttct catctttctg acttccttga tactctccca
      361 attctaaaaa gtgattcaag gtagtataaa attccttatt ttttacttca tcaatgatga
      421 ccatatcata atcttttgtt gtgcgacttt taaatccttg cgaatccaat acgatagagg
      481 tagcagttcc cccaatcaga acataatagt tctgaaaatc cgcaaacatt tcttgaaaaa
      541 taactttcgt attagctggc atcatcttct cccagatact gtaatatcat attttctagt
      601 gcttcactct cttcctctat acgtgggtca tcatcatctt ttaaggtcaa ataaagagaa
      661 atcggatcta caaattgttt atcatgatta tttttaaaat cattccaaaa ctcagataca
      721 aaaggacgat atttccatat ctctagcatc tttcatttta aaacatgctg agaaagtggc
      781 aaggataact gattgaattt tctctgccat atgacatagc tagtattttc atccgtttca
      841 gctaaaaaag ttgaatgcga caaagcataa gcaccaccat atagaaggtt agaaacagat
      901 tttatctgct ttatatcgcc atctggcaat aaaatccgtt ttttgatggg attaaataaa
      961 catgacacgg attttaagaa taattctttc tttgacaccg tatatgtgta aagcttattt
     1021 tgcttgttta accaatataa agctttaaaa gtcctcaaac acctataaat tgttgagttt
     1081 ggaagtccag tgacttgtga aagcaaatca acatttacta ctttttgacc ttttgtcaat
     1141 aaaaaggcaa tccacgttaa ttgttcgcta ggtgttaatt ccttagggat ttcagtatca
     1201 ttcgcattga gtactagtcc caatggaggg aagaagaggt ttcccttaaa gtctacaaac
     1261 ggaactctag cttgaagtaa ttgctttttt tcactgtctg ataacttcga aaacaccaaa
     1321 acaacatcca tattggcttt ttcacccata gtgcgagcct gagtaacaaa tgaactcaaa
     1381 ctcccccttc tcttttcttt aatcaataaa aatgattcct tatgaaaagt catttgagta
     1441 tactcaaacc tttttacaaa actgattggt aaagtcaaag tcagttcctc aatactgaca
     1501 tcaacataaa aggattgaaa aattgacatt atctttttga cattcatgtt ttttccttat
     1561 tctatcattt ttattatatt ttatcattcg gtataataaa atataagttt tttgataaaa
     1621 taaccaaaaa ctactcgcta tcccctctca cattacccta catatcgttt gacatgcgac
     1681 tgatagttca ggaaaacttc aaggagaact tttctctcat ccactatgca ggacttacta
     1741 tttcacttct actcctatag gctcaatttg acacttttct ttttcgaatt gctcatatgc
     1801 ctcctgaata gtgagttcga tttatccaaa tttatttttg tctattttga tgaatacttc
     1861 cacgaagata gtctgaaaat gaatttgcac caattttcct tctcttttta agcatattat
     1921 tccagatatg attgccctct tattcagtaa taaattacgc tttaaacgct tcaatcagta
     1981 tatctcctgt tgtcatatgt tctaaagaaa attcttctac atatggttta acatctctta
     2041 ggttattact tcctaatatc ccattatgct ttttcgctaa gaaaatagaa gtcgcttctc
     2101 ccttaccaat aatcttgtta ctatcatgat ttcttgttaa atctctatat aatgcgtatt
     2161 cttcagttcc aatgtctatg ctcacaatct cagctgaacc cttagttacc aactggtcta
     2221 tcctagattt taaatgggga attgtaggta tattgatttc atcatacacc tcttgtggaa
     2281 taacaatttt acccaaatag agcttttcta aaagatgttc agtgccaacc cataaaaata
     2341 ctgaaataca atctgcatca ataaatacac gactagtcaa acgacaactc ccccctcttc
     2401 atctagccca tatacaatat catatctgaa agcatctagt aacagttcct catacttccc
     2461 ttgcgaaatt ctgttatttt ctaaaagttg ttcagttgaa ttaatatatt atcctaatgc
     2521 catttctttt ttactttctg acaaaggacg atataaactt gtatcatagc ctaatcttga
     2581 agctgtctct ataacactaa tatccatatt tttaatttct tctgcatcaa ggtatccatc
     2641 attcctcaat ctatataaca tagctttatg actgatacca taaaactgac ccaattttat
     2701 aatatcttct acttcaagat gagttctatt ggcattttct ctgatttcct caaccatcct
     2761 atacagtgaa gatgggaaaa ttaaaaaata agaagcaaac tgatccgctt ttctttcagt
     2821 ttcatctcct tcaccaatca agataagact gactgaactc ttcttcacct catcataata
     2881 aagatgatac agttcatgtg ctaaagaaaa tctttacctt cctaatggca tgtctgaatt
     2941 gactgcaatg agactgaact gagttccttt ataacagact ccgctaatat tctttccgag
     3001 tccataaaat accagcgtca aattttctat cttttgtacc aatttaaaaa tatctatcgg
     3061 cgattcacca tcagctccca acttttttct aagatttgaa gctttattac ttaaatccat
     3121 tcgtgatatt cctttcacct ttttccattt tggcaatcat gctttgattc aaagacaaat
     3181 actcagcaat cttatcttga gtaagatttg aagatattct aagttctttt attctttttc
     3241 caacatcaca tacatttatc acatacattt atcacataca tttatcatat tcatacacct
     3301 ctcataaaaa gaatagcaca ctcttgtcat aattttttaa ataaaaaaat tatgacaaaa
     3361 caaggaagca atttattgat gctgcttaaa aatctaaaat tgatgagttc ctatcaaaaa
     3421 tcgtatcttc aacctcaaaa cagtacttaa agctatccga ctcggtttac attgtcaaat
     3481 ttagatttta tttgagcata actttctagt ttgctttctg atttttgttt aatatagtag
     3541 caaacattga aggaaatatc tccacaaaaa aacgcatact attaagcttt ttcaagacct
     3601 aataatatgc gctgttctga tttgaaagac attccattat tattttactg taatcaagcc
     3661 atctggctct actgtgaatt ctggcttgtc tgccagtgtt ccgtctggtt tgaggtagta
     3721 ccagcctgtt ccgtccgctg actggataaa ggcatttgat accatggcgc cttctttagc
     3781 gtctaagtag taccaagtgt ccttgtactt gacccagcct gtcttcatgg caccttcttc
     3841 gttgaaatag taccacttat cagcgatttt cttccagcct gtagccattt cgcctgagtt
     3901 gtcgaaccag taccagttgc cgtctgtgtg cttcctccag cggtctgcaa gcatatagcc
     3961 tgaactgtca aagtagtacc aagtgccatt gattttctca aacttgtctt ttggataaga
     4021 gccgtctgag tgtacgtacc agtagccagt gtcattcttc tgccagcctg tttcaatcgt
     4081 caagccgttc tcaatatcat gcttaaactg ctcacggcta atgccccatt ttgccaagta
     4141 agggtatgga tccacatggt ctgagtggtt gtttggttgg ttattcgtgc aatactcgtg
     4201 cgttttaatt ccagctaaac tccctgtatc aagcgttttc ggcaaacctg cttcatctgc
     4261 tagattgcgt aagagttcga tataaaggcg gtagtccgtc atgaactctt ctttagttga
     4321 atggctttca atcagttcaa ccgctgcata ggtctcagca ttccaaccgc ccccaacgtc
     4381 ccaggcacca ttatcaacag gtcctacctg catgatgcaa ccgttcccaa caatgtgcga
     4441 gaaaaaacct aattctgggt ctttccgcca gtgataatcc gcttcattct gtacggttga
     4501 atgcggattc ccagttgagt gtgcgtgtac ttgcctatat ggttgcacgc cgacttgagg
     4561 caaatctgtt cttaatttac tcacattaat ttccatattc tactccttat caattaaaac
     4621 aactcatttt ttacaatcca gaaccagaaa ctcctttatt tctaccttac aaagaagaca
     4681 attttagtca cgattaggct tgtagataga acttcaaaac gcactatttt gacactgtaa
     4741 ataggactga caaggtctgc attctatcta caataacacc ccaaactaaa aagcttttca
     4801 aaagtatatt tttacagtct ctatatgtcc ttttcataaa tactatactt tattatatca
     4861 tataaaagaa gtcaaaagtc tgttaaacta ttttcaacac caaactaaag aagagaacac
     4921 aagttttttc gattttcact agaggaaatg gattttattc agtaaatcca actaggattg
     4981 cactttggtt gccaaaattg cctttccttc ttttatcaag ggatgacgga atagtgagaa
     5041 gtacagttga actgtcatgg caacccagat aagaggttca caaaggataa cacccttata
     5101 tcctgcccaa ggaataatca aaaccacaaa aacgattttt ccgattagtt caataaagct
     5161 agaaactaga ggaaggatct tttgccccaa gccctgcaag caattgcgat aaatcaacaa
     5221 gaggctcaaa atgggataaa aggctgaact gatttgcaga tagagacttc cattttctat
     5281 caagtaacca tctgtcgaac tagccaagaa ggaaaccaaa gctggactgg ccaaaaagag
     5341 gaaaatacaa acaaaaactg cccaggatat acttaaacga ctgccgattc gaagaccttg
     5401 aacaatacgg tcaggtcgct tagctcctag attctgagaa gcaaaggtcg tcattgatgc
     5461 agaaatagcg gtcataggaa gaagggcaaa ggtcataatg cgtcgagctg ccgtctgggc
     5521 actaataatc actgcaccaa atgtattaac agaaaactgt aaaatcacac tgccgataga
     5581 tacaattgaa ctcatcaagc ccatagccaa accttgctcc aagagatccg cgtacaagct
     5641 tttgtcccat ttgaaatgtt taaactgtgg caagagttct ggcacacttt tacgaatata
     5701 ataaaagcag agaaccgctg ataaaccttg cgaaataatg gtagcaagtc ctgcggattg
     5761 aactcccaga tgcaattgcg taataaaata gagatccaga accacattaa ccaaggcaga
     5821 gaaaatcaga aatcccaggg ctgctagact gtcaccaata gaccgcaaca agcctgcaaa
     5881 aagattataa gcaaagctga cacctacaca ggtcacaatc atagaaatat attgataaga
     5941 ttgaggaaga atttctgcag gagtatctaa gtattgcaag agaggataca agccaagaaa
     6001 gcccagcaac ataactagaa tgctcaaaag agcacctaaa atccaagtgg ctgctactgc
     6061 ttccttgatt ttagtgaaat tccgagctcc ataataacga gcaatgacaa tccccatgcc
     6121 attgccaaca ccaagtgtaa aacctacaat caggtcaaaa atcgctgtcg tcgctcctac
     6181 tgcagccaag gattcttgac caagaaatcg tccaacaatc aagacatcag cagtgttata
     6241 gagctgttga aaaatatttg atagcaagat tggaaaggtg aagcttaaga gcgagggaag
     6301 aatcggacca tgtatcaggt ccactgttcg ttttttattc ataaagctat tatatcagct
     6361 tcctagagga gcgacaagaa acagcaaact tatatatagc ggaatgaaat aaggacagtc
     6421 aaatcgattt ttaacaatgt tttagaagta gatgtgtact attctagttt cgatctatta
     6481 tagttggttg agtttggaat agtacgctgt agctgctaaa acatttctag aaattaattt
     6541 gactttccta ataaagttgt tcatatctta tttcaagcta ctatatataa tctaaaaact
     6601 tgaatcaaaa atcgaaaaag tagcggattt ctccactgct ttaacagctt attcttcaat
     6661 ttcgatttca agttcatcgc ctaagtcaag cgtaacttct tcattcactg cttcttcttt
     6721 ctttggctca tcttttttgt tttcagtatc ttgttctgaa acttcttctc catcaatcaa
     6781 gccaaattta gaacggactt gcttatcaat ttcatcaaag atttctgggt gctctgccaa
     6841 gtatttctta gcattctcag aaccttgccc aattttttca tctttgtaag aataccaagc
     6901 ccctgctttt ttgataatat ccaaatcgct tgcaatcttc aaaagctcac cagtcttaga
     6961 aattccttct ccgtacataa tttcaactac ggcttcctta aacggtggag ctaccttatt
     7021 ttttacaacc ttaatcttag tttctttacc gacattggtt tctttttggt caccagttcc
     7081 cttaatttgt gtattaccac gaacatccaa gcggactgaa gcatagaatt tcaaagcacg
     7141 tccgcccggt gttgtttctg gatttccaaa catcactcca actttttcac gcaattggtt
     7201 gataaaaatg gcaattgttt tggttttatt gatagaggcg ccaagtttac gcatggcctg
     7261 gctcatcata cgagcctgca aaccaacatg gctatctccg atatctccat caatttccgc
     7321 acgaggaaca agggcagcaa ctgagtcgac tacgacaaga tcaactgcac ctgagtcaat
     7381 caattttccc gcaatctcaa gaccttgctc tcctgagtct ggttgagaca agagcaattc
     7441 gtcaatattg acaccaaggg ccgcagcata agctggatca agggcatgtt ccgcatcgat
     7501 aaaggcagca atcccacctt ctttttgcgc ttgtgcaact gcatgaaggg caaccgttgt
     7561 cttaccagat gactctgggc catagatttc gatgatacgt cccttaggat aaccacctga
     7621 gccaagggca atgtcaagag ctaaagaacc tgagctcatc acttgcacct tttgctccgc
     7681 acgttcaccc aaacgcatga ttgatccttt accaaagtct ttctcaatca atttaagagc
     7741 gtcattcaag gccttttcac gttctgcccc aaattttttt gaaatttctt ctaatttttt
     7801 tggttttttc gccattctat tctcctacat tctaatgatc ctcagaccta tctactatta
     7861 tatcaaaagt tagtcactta ataaagcctt gcgaactagg ttaaaggcat gcataaccgc
     7921 aatgtgacgt acatctgctc ggcttctgcc tccaatatta accttgataa cctcagttcc
     7981 ttgatcttgc gccaagccta tgaagactgt cccaactggg tgtccttcta ggctatctgg
     8041 tcctgccact ccagtcaaac taattccaaa atcagactgg gtcttgcttc gtgcctgctc
     8101 agccatcttc tgagctgtaa attcagacac cacaccatgt tcttccaaat tcttggcagg
     8161 aatatccaac atccttgatt tttcctccaa gctataggtc acaaaaccac ccttaaatat
     8221 acttgaaact ccagaaaaat tcgccacggt agcttggaaa agacctgccg tcaaactctc
     8281 tgcagccgcg atggttttcc cttgcctttt cagttcttct accacaatgc tggctaaact
     8341 agtttcttcc ccataaccat agcaaaagtc tcgtaaagaa attccttcga aagtctggca
     8401 gtccaagatt tgattttcca agatatccag cgcttgattc gcctcttctt gactgctagc
     8461 ctttgttgac agacgtagag tgacttctcc tgtcttggca taaggggcca aggtaggatc
     8521 gatctgatta tcaattaaat cagccaaaat cgtaaccaac tggctctcgc caatcccaaa
     8581 gaaacgaaga actcgggaat acagcttgct ccctgtcatc aacttgggta gaagttggtt
     8641 taagaccatg ggtttcaatt cacttggcgg acctggaagg acgacatagg tcactccgtc
     8701 tacttctaat tttcctccaa cagccagtcc tgtttcgttt ggcagtggaa tcgctccttc
     8761 tacaatttga gcttgtcttt cgttattcgg tgttcgggca tagtctggtc gcagggcaaa
     8821 aaagatatcc aacttctcct gagcctgagg atcaaagact aatgctttcc ctaaaaattt
     8881 agctagggtt tgtttggtta ggtcgtcctc agttggcccc aaaccgccag tcaaaatcac
     8941 cagactgcta cgttgactgg caatctcaag caaagacaag agacgaactt cattgtctcc
     9001 tacagccgtc tgaaaatata catctacccc aatctcagct agtttttccg acaaaaactg
     9061 ggcattggtg ttgacaatct gtcctgtcaa aatctctgtt ccaacagcaa tgatttctgc
     9121 tttcatgttt cctcctacct atctattcgt atttttttga aaaaatcgca ggaattttcc
     9181 tacgattgat ttttttattt gtatcaaaag ttaattatct tcatcaccaa caggtgctct
     9241 gccaaataaa tcttcaaata aaaccgcatt ggtttcaagc tgagtaactt cttcttgtcc
     9301 caaagaacgt cggagtatat tttgcatttc caacatatgt gctctcgaaa caatctggta
     9361 agaaacacct tgaagtatct ctccttcacc ctgcaactgc tgagtttcaa tggttttaaa
     9421 tgaatcttta tagcctagca agttagggat actttttgca gacaaatcaa tattggtctg
     9481 catattgtca ctcaaagctt ttagaatctc ttgataatga ccaacgctat ttaaactgag
     9541 agctttttcc atgacttttt gaataacttc acgttgacgt ttttgacgac cataatcccc
     9601 ctcaggatct tggtaacgca ttcgtgcata gactagggct tcttctcccc caatatgttg
     9661 ctccccaaca ccgatagaaa tagtattaaa ttcttcttgg tcactgatag aaattgggaa
     9721 acctaggata ttattgactg taatacctcc tactgcatcc actagttttt gcaaccctct
     9781 catattgacc atcacatagc gatcaatatg gatattcatc attttttgaa tggtttctat
     9841 agcaagctct gctccaccat ctgcatatgc tgagttcagt ttcgcttcat gagcctgacc
     9901 attccctgat tcaatgcgcg tcagaatatc ccgctctaaa ctcatcattg ttgttttttt
     9961 cgttttagga ttcactgtca tcaagatcat gctatcactt ctaccgaccc aagtttcagt
    10021 tcgttcagca tttccggtgt ccactcccat taacagaatg gttagaggtt cagtcgcttc
    10081 aataaccttg gtttcttcgc cgattttttt ataggtttta gctaaggttt ctgtcccttg
    10141 ttgataaata gtataagcaa aaacacctac tcctactaca gttacagaaa gtaaagctag
    10201 caccattcca ataatttttt taaccatatt tctactaacc tatcagttta cccatcaagt
    10261 aaacatcgat aaatttccct tcttctatat atgccccacg ctcttggcta ccttcaatga
    10321 caaagccatg cttttgataa agatggactg ctgcttgatt acgagtttgg acagtcagtt
    10381 ggagacgacg cagaatgcca cttgcttgtg cccactctat cgcttcttct agcaacaaac
    10441 ttcccaagcc attattccaa tatctttttc caatcacaat gaagagatct ccaatatgac
    10501 ggactctctt acgctgatca gctgtaatat ttacaatacc agcaattttg ccatttaaga
    10561 atgcaagtaa ggttatctga ttgtccgaac tagcttgctt gttgaggaat atttccatct
    10621 cctcactagt caagagaata ccatctccgt ctaggctggt aaagtctgtc tccaaactca
    10681 cacgatttaa aaaggccact aattcagctg catctttggg ctctgcttcc ctaatgagca
    10741 attcatactc catattgaag ctcctctaac aatttctcag cacgcaaacc ctttgcctga
    10801 aaatttaaac ggcgtccatc tgcttctttt agaatttcca attctaaata agcatctggc
    10861 aaggcatctc ctaagagatt tccccactca ataacagtca cgccgccacc aaagataaac
    10921 tcatccaagt cgatagaatc agcatctcct tcaatacgat aaacatctag gtgataaagt
    10981 ggaagtcgac cctcatactc tctcacgata gtataggtgg gacttttaat catttgagaa
    11041 atctgtaatc cttttgcaag tcctttagta aaggtcgttt tacctgcacc cagttctcca
    11101 gttaagatta aaacatcatt ctttgctaat agatggccca aacgctcccc taaggcttgc
    11161 aactcttctt cattttttgt gtacatactc ttattatacc aaaaactttt cttttgtgtc
    11221 tattttccta ctaaacttat catcataaca tccataaaaa acaggctttc tctaaaagaa
    11281 aatgagcgta acaatgacca atacaagatc tcggaaaata tgaccataaa aggaaacttc
    11341 cttcttaacc gaatttggga caagataggc tgcaaaaaac aagcccagtc caatataaat
    11401 cagaagtgag acaatggtca ttggatttct taagaaaaga agtgttgcta aaatagtcac
    11461 caacactgtc ttttttctgt ccagcatagc aagaaaatcg cgcacgtatt ttttcaaggg
    11521 taaaaaaatc agcaaatcta gcccaaatag gaaaaagaag gatggcaata aaaagtcaac
    11581 taattcttgc tgcagcgtat ttttgatgaa caagttatct gacaaaacaa gaacagctcc
    11641 taacaaatta attaagagta acatactgta aaaaagcttc accgacttct tactggctag
    11701 gacactatgg acttcttgct tacgagtata aagataattt actccagcac agattcctga
    11761 aacgaaaacc atgcttccga tgaaaaaagc tgtactttgt ttaaaggaca agatgcattc
    11821 cttccatagg aaacagctac tcaaactgat ttgaattaaa gctaacaaaa ataagattct
    11881 cattgatttc atcttctctc tcccttccta ccaatcatta tactaggaga aaagagagaa
    11941 ctgtttctaa tcttctcaaa tgtctcttta agacgctaaa caaacactag agactaatac
    12001 tcaatgaaaa tcaaagagca aactaggtag ctagccacag gttgctcaaa acactgtttt
    12061 gagattgcag atagagctga cgtggtttga agagatttac gaagaatata aatttgaaat
    12121 catgaaaatc cgtcaaacgg gtggttgttt tgtctcgcac ctcacggagc gagacggact
    12181 cagagtcaca taattataag gctgatagta ttaatctaac tatcagcctt acaggttatt
    12241 taacgtttca gaaaaactat aatgtcaaga ttaactaaac agtatctagt tccttcaaat
    12301 aattttctat cttcatcaac attaaaggat tgttataaat cttacataac tctcttgctt
    12361 ctatataata atttttgact tgttctctgt ctagaaattt ggctccagca tttcctacaa
    12421 gaataagtag aggagccaat tggtagcttg tctgtctttg tttacagagt tcaatcgttt
    12481 caagagcttc ttggatggct tcattatatt tttcctttga tactaggtag tgagcgtagt
    12541 tgtaacgaac tctgatgtag ccaaataaaa actcttgatg gtccaaattt tttgtctgat
    12601 acaactctat taaatgagag tagtttgcct catattcttg ttcacgaccc actaaggaat
    12661 agaaattaga tagagtattc aacgccttta aataaatcag agtatttgaa gagactttta
    12721 ataatatatt ttccaatgaa gaaattgcct cacacttact gtcatattga tagaagtcaa
    12781 taatagcttt aatccattca aggtaagttc ggtcttctag tgttagaaaa gtacttcgtt
    12841 caatctctat tctataaata tattccaaat cgtcataatt tctatcgtct aatagtcgag
    12901 cagacaattg cttgaaatta gagaggttag atttaatttc aatttgttca ttaaaaaaat
    12961 aatctaatgg tacttcaagt cgttgagaga gtttgaacaa aaggtctgcg gagggaatga
    13021 aatgccctct ctcaatttta ctaatctgac tttgttcaca aattccttct gcaagagttt
    13081 gttgggagag tctcaactct tttcttttca atctgaattt ctctgcgagt gtattcatta
    13141 aagataataa accttcctat ttgcttaatt gcattataaa atttttagtt caaaatagca
    13201 agttaaagga ataaataatt ctttatggta ataaactaaa aaaagtcaaa aattacataa
    13261 aaatgcttgg ctaaataaaa tatatctgtt aaaataagga taatgaaaaa gaaaagggtt
    13321 tcacgaatga agaaaaatcg tggaattcaa aaattagcta tattagtatt actaggtgtt
    13381 tttatgttta gtaatacaat tccttaccaa cagtttattc agaagaatag acaattggag
    13441 attcgagtgc aatcgcaaaa gaagtccaat ggtcttgatg ttgggaaggc tgattgattt
    13501 cgtattactt taaaattcaa ctatttagag aaaggtattc tgatgagagc ttgtaacaat
    13561 accagataga tataagaatg gagatgtttg ataaaatgct ttcatagcta accaactgtt
    13621 gaaaattatg agttacaaaa tctaaagtat ttaagattgg tttataaaat taggtctctg
    13681 cctgtagaaa ggaacgtgca gttagaaagg aggaaaaaat aggatactat aattcagact
    13741 gagatttcta attaaaatat atagttgcat ttcgctcatt ttcataatat ttcattcaga
    13801 ggagtaataa tatgttaaaa tttagaaaag tagttgcatc atcccttgcc gttttggcat
    13861 ttagtttggc tcttcctgtt ttggcagttt ctcaccgtgg tggcgagtgg acctatggag
    13921 gccatcatga tccaaataat tggggagcat tttcgaatta ttatcatggt tcacagtatc
    13981 actgggccta tgttggaagt aatggacgta acaatcaaaa aacagtttat gcaggagcac
    14041 gtagtgcggc ctatgcgttt gttaatacaa attttgggga gcaagttaca tttgatgcgg
    14101 gatggtaaag aataattgaa gaagattctt ttatcaatag ttcagtaagc ttgttagttt
    14161 atagagggca ggagtatttc tgccctctgt tttagaataa ggaatgcact atgaaaaaat
    14221 tatttatact catatccaac ctgcttgcta gtttgttctt tgtttgggta tttaccattt
    14281 ggactgatac atatgtctcc cattattacc ccaacgttgt tgtacgtgat tcttcccctg
    14341 aaacaacttt tcagcatgtt gctacacgct tggaaaaact agcagaagaa acagatagct
    14401 ttattgcgat tcaacaccaa gatcctaact cagaaggtac tacagttttt tcttatacga
    14461 cctttgggga cggaaagttg cctgatgggc ttcaggaaaa aaatctagaa gatgctcaaa
    14521 gtagtagtgt tgaaacaaac tattttgtat tcgatggaca cctagatatt cacttgctaa
    14581 gggaagagct aagtcaactt ggcttgacta acatgcacct gacaattcca tctaaactat
    14641 ctacgttaat ggctatcttt agtaacggat ttcagttaat cagtctattg attttcatct
    14701 taacctttgt agcactgact ttaattagtc aaattagcca attacggtca tcgggcattc
    14761 gcctcatttc aggagagaaa cggtggtcca ttttccttag accagttggt gaggatttga
    14821 aagggatagc tgttggtttc agtttagctg gcgtgctcgc cattcttatg caaaaaattc
    14881 tgtcgcttcc aacgcaatcc ctaatgacca taggagcagg cttgctcagt tataatctca
    14941 tcttgttgtc gatctccctg tttttcgctc aactcttcgc agttgggata aaaaagatac
    15001 acctgatgca gattataaaa gggcaagtgc ctgtcagagg aatcattagt ttgattttaa
    15061 tcggtcaact gttagcgatt attattgtaa cgctgggaat agggagtagc ttaaagtatt
    15121 cccaagcctg gcaacagcat cggattggac aagaggtttg gagtcaggaa agacaactga
    15181 ttaccctatc aatcagccgt gaaggaacga gtcctggttt tgatgaacaa gctcaaagga
    15241 aactcagaac ttggtatcaa ttgatggatc tggctgtttc agaacaaaag gctttcttat
    15301 ctagacacca gttaattgac cgtactttgc aaaatggcat ggcttcctcc aaaaacttga
    15361 caacctctac agaatggcac gattacagcc cgaatggcaa tgtccttatc gtcacaccgc
    15421 attacttgga gcgtcaaaac attcctgtag atacaactat taagcaaaag atgaatcacc
    15481 ttaatgtagg ggagtttgtc ttattgctgc ctgaacacct ccgttcagag gaagaacatt
    15541 ataaatctgt ttttgaagac gacttaacca gtcgcatgtc tagtcaagat gaacgacagc
    15601 aaatgactgc tacggtaggt tatttagaat caggtcagga tcgttttgtg tataatacga
    15661 cccctatttc ttaccagcag tttttgaaag atccaatcat cattgttata acaccccaat
    15721 caactggtcc acagtccatt ttgttttgga tagacgcagt acagaactac gttctcttta
    15781 atcaattgtc tgatgcccag gagcttatcc agagacaagg cattgaaaat tgggtctcag
    15841 aaatgcaaac aggttaccac aactacatca cattattgga taatatccag agggaacgtt
    15901 gggtaatgct agcaggagct gtgcttggga ttgcaacttc aatcttgttg tttaacacta
    15961 tgaataggct ctactttgaa gaatttagac gtgccatttt tatcaaacgc attgcaggtc
    16021 tcaggttctt agaaatccat cgcacttatc tctttgctca actgggtgtg tttttactgg
    16081 gatttgttgc gagtgtattt cttcaggtag agataggagt tgctttctta gtcttgttac
    16141 tctttactgg tctatctctt ttacagttac atgtccaaat gcagaaagaa aacaagatgt
    16201 ccattcttgt tttgaaggga ggttaatatg attgaactta aacaggtgag taaatctttt
    16261 ggagaacgag agttattttc gaatctttca atgacatttg aggctggaaa agtctatgcc
    16321 ttaattggtt caagtggtag cggaaaaaca accttgatga acatgattgg gaaattagaa
    16381 ccttatgatg ggacgatttt ttaccgaggt aaagacttgg ccaattataa atcgagtgat
    16441 tttttccgtc acgaattggg ctacctcttc cagaactttg gcttaattga aaaccaaagt
    16501 attgaagaaa accttaagct aggtctcatt ggtcaaaagt tgagtcggtc ggaacagcgg
    16561 ttgaggcaga agcaggcttt agaacaggtc ggcctggctt atcttgacct agataagcgc
    16621 atctttgagt tatcgggcgg agaatcgcaa cgagttgcct tggcaaaaat tatcttaaag
    16681 aatccaccct ttattctggc agatgagcca acagcttcaa tagacccagc aacctctcag
    16741 ttgattatgg agattttgct atctcttcga gatgataata ggctaatcat tatcgcaaca
    16801 cataatccgg caatttggga gatggctgat gaagtgttca cgatggatca tctgaaataa
    16861 aaatccttgt ttttaattgc acgatgagtt actgaaatat tatcatgaat caataattgg
    16921 agttaattta gaattgtact ttattaatat tgaggtaact ttttcttgat aaaggaggaa
    16981 atagtggaga ggaagttaga atgaaaaaat tcgacaatta tattattgag aagccttgcg
    17041 attctaattc agataaactg caaaaaatct taataattga aaatttggta gatgatattt
    17101 tgcaatttcc tctcagaatc aataatagtg taggagagat tttcctccta caaccgtttc
    17161 aaaagaaaac tatctttatt ccatgttatt ttgaggaaga tattgtgaaa gtcaaagatg
    17221 atgataaagt tgagtggaat ttgttagaat ttcaaaaatt tagagcattt ttggcttagt
    17281 aatctgtgtt gaaggctcaa aacctatggt aaaaaagtcg ctttgaaaac gtattgcctc
    17341 caaagattta gttaaataat gatttaacac aaaaagaaat tattgaagtt ctggaaagat
    17401 gttgtttcag tattgagaaa aggtgggaaa aacttgcgat tttcacagag aaaggaagaa
    17461 aaagtataga aatatagtca attgaaacaa gaacaggaca aaagaacctt ttgtgccata
    17521 tttttctcct ttcgctttac aattggcttg aacaccttta ttgtatcgcg tttggagttt
    17581 ttttggtata accttcgacg cgcacccgca tagcgggtgt ttttttgtct cgcacctaac
    17641 ggagcgagac gaactaatag tcacttaatc aaaaaacgca ccatatcaaa aactaaaaag
    17701 tttgatatca tgcgtcatgt cttaaactaa ttgactatac tttctattca aatgagcttt
    17761 taaccaattg attgagccaa tccactctta aaaccaaaga gcaatttctc gcttagctga
    17821 ctcttctgaa tctgaaccat gtacaacatt ttggataacc tcattttctc cagcagcttt
    17881 tgcaaagtca cctcgaatag tgcctggtaa agattcttct ggacgagttg cacccatcat
    17941 ggtccgccaa gtttcgatta ctttgggacc agaaatgaca cccacaagaa ctggacctga
    18001 agtcatgaat tcacgaatcg gtaggtaaaa actctgacca accaagtcct gatagtgctg
    18061 gtcaatcaac tcttctgaaa cctgtgaacg aaactccaat ttttcgattg taaatccacg
    18121 ttgttcgatg cgctttaaca cttcacccac tagccctctt tttacaccat ctggtttgat
    18181 gataaagaat gtttgttcca tacccgtctc ctttgtcagc ttctttcttt tattttacca
    18241 catttcgtgg aaaaatggag aaagttttca gaagagagaa tgagagaacc cgagggttct
    18301 ctcattctct cttattctac tgtttcttcc acagtttcaa cggcagtatc cacaactact
    18361 tctgttgttt cttcatttcc ttcttcctct actggaggat taaggtattc ttcttcgttg
    18421 acagcatgtg gttcaaggtt acggtaacgg gccataccag taccagctgg gatgatctta
    18481 ccgatgataa cattttcttt aagtccaagg agatggtctt tcttaccacg gatagctgcg
    18541 tcagtaagga cacgagttgt ttcttggaag gaagctgctg acaagaaact gtttgtttca
    18601 agtgaggctt tggtaattcc cataaggact gggcgacctg tcgctggaac tccacctgcg
    18661 ataaggacat ctttgttggc atctgtaaag tcattgatat ccatgagggt acccatgaga
    18721 agatctgtat cacctggatc catgacacgg actttacgga tcatttgacg aaccattacc
    18781 tcgatgtgtt tgtcaccgat ttctacccct tggctacggt aaactttttg tacttcaccg
    18841 agaaggtacg tttcaactga caagacatca cgaactgcaa ggagacgttt tggttggata
    18901 gaaccttctg tcagagcagc accacgcgct acttggcccc caacttcgac acgcatacga
    18961 gctgtaaatg gaacgacata ttcaccttcg ccagtttcac ccttaacaaa gactttcttg
    19021 gtacgagttg atgcatcttc ttcgatagca gtaacttgtc ctttaacctc tgtgataacc
    19081 gcttcccctt taggattacg ggcttcaaag atttcttgga cacgaggaag accctgagtg
    19141 atatcggtat ttgaggcaac cccacctgtg tggaaggtac gcattgtaag ctgtgtacca
    19201 ggttccccga tagattgggc agcaattgta ccaactgctt caccaacttc aaccgcatca
    19261 ccagtcgcca agttgatacc gtaacagtga cggcagacac cgtgacgagt gttacatgta
    19321 aatacagaac ggatagtcac ttcttccaca ccagcattga caatttcacg cgccttgtct
    19381 tctgtaatca attcatttgg accaataatc actgcaccag tttctggatg tttaacagtt
    19441 ttcttagtgt aacgaccgtt gagacgctct tcgagagact cgatcatctc ttttccttct
    19501 gcgatagaac ggatcaagag accacggtca gttccacagt cgtcctcacg gatgataacg
    19561 tcttgggcaa cgtcgaccaa acgacgagtc aagtaacctg agtcggctgt cttaagggcc
    19621 gtatcggtca tacctttacg agcaccgtga gttgagaaga acatttccaa taccgacaaa
    19681 ccttcgcgga agtttgaaag gattggcaat tccatgatac gtccattcgg agcagccatc
    19741 agaccacgca taccggcaag ctgtgagaag tttgagatgt taccacgggc tccagagtcc
    19801 atcatcataa cgattgggtt cttaggatct tgattagcaa tcaagcgttt ctcaagtttt
    19861 tcacgggcag cacgccattc agctgtaaca gcattgtaac gctcgtcgtc tgtgatcata
    19921 ccacgacgga attgtttggt gatttgttcg acacgtttgt gtgattcttc aatgatttca
    19981 gccttgtcat caacgactgg gatatcggca atacccactg tcaatcctgc aagagttgag
    20041 tggtggtaac cgaggttctt catgcggtca agtagggcag aagtttctgt cgtacggaaa
    20101 cgtttgaaga tttcagcgat gatatttccg aggtttttct tcttgaatgg agggttgagc
    20161 tcaagattgc tgatagcttc cttgatatct ccaccaagtg gcaagaagta tttagctgga
    20221 acaccttctg tcaagttggc attgtttggt tcttgcaagt atggtagccc ctctggcatg
    20281 atatcgttga agagaatttt accaactgtt gtaagcaaga ccttatgtct ttgctcttct
    20341 gtccaaggct tgttgaggct gtctgttgcg ataccaacac gtgagtggag gtgaacataa
    20401 ccattgcggt aagacataac cgcttcgtca cggtctttga agaccattcc ttcaccttcg
    20461 cgaccagctt cttccatggt caagtagtag ttacccaaaa ccatgtcctg agatggagta
    20521 actaccggtt tcccatcttt cgggttcaag atgtgctcag cagctagcat gaggatacga
    20581 gcttctgctt gtgcttcttc tgaaagtggt acgtggatgg ccatttggtc cccgtcaaag
    20641 tcagcattgt aggcttcaca gacaagtggg tgcaagcgaa gagccttacc atcaattaag
    20701 actggctcga aggcttggat acccaaacgg tgaagggtcg gtgcgcggtt caaaagcact
    20761 gggtgttctt taatcacttc ttcaaggata tcccagatac gctcatctcc gcgttccacc
    20821 aagcgtttag ctgctttgac gttttgcacg atatcacggg caacgatttc acgcatgaca
    20881 aatggtttaa agagttcaat cgccatttca cgtggcacac cacattggta catcttaaga
    20941 gttggaccaa cggcgataac tgaacgtcct gagaagtcaa cacgtttacc gagcaagttt
    21001 tgacggaagc gtccttgttt acctttaagc atgtggctca atgatttcaa tggacggcta
    21061 cctggtcctg tgattggacg accacgacga ccattgtcaa tcaaagcgtc aactgcttct
    21121 tgaagcatac gcttctcatt ttgaacgatg atacctggtg catttaactc aagcaaacga
    21181 gccaaacggt tgttacggtt gataacacgg cggtaaaggt cattcaagtc agatgaggca
    21241 aaacggccac catccaactg caacattgga cgaagatctg gtgggataac cggaaggatg
    21301 ttaagaatca tccattcagg tttgtttcca gacttgtaaa aggcatccaa aacatccaaa
    21361 cgacggatgg ctttgacacg tttttgtcca gtagctgttt tcaattcttc tttgagttca
    21421 gcaatttctt tttcaagatc tacttgcttc aaaaggtctt ggatggcttc cgcacccatc
    21481 ttggcaacaa atgaaccata accatattca cgcaagcgct ctcggtattc gcgctctgtc
    21541 atgatagact tgtgctcaag tggtgtatcc ttaggatcaa tcaccacata agccgcaaag
    21601 tagataactt cctcgagggc acgagggctc atatcaaggg tcaagcccat acggcttgga
    21661 atccccttga agtaccagat gtgagataca ggagctttca attcgatatg tcccatacgc
    21721 tcacgacgaa ctttcgtacg cgttacttca accccacagc ggtcacaaac aattcctctg
    21781 taacgaatgc gtttgtactt accacaagca cattcccagt cttttgtagg accaaagatc
    21841 acttcatcaa agagtccttc acgttctggt ttcaaggtac gataattgat tgtttcaggt
    21901 tttttgactt ctccataaga ccatgaacgg actttacttg gagaagctag ggtgatttgc
    21961 atacttttaa aacgatttac atcaaccact atttcttccc tttctattct aagtgaactg
    22021 cttattcttg ttcagcagct tcttctgttg cttccgcttt tgttgctttc tcagcttctt
    22081 cagcttcaaa ggctgcttta gcctcttggg ctgctttttc gcgggctttt tcaaggtcat
    22141 ctacgtggat gacatcttcg tccattcctt catccaagtc gcgaagttcc acttcttggt
    22201 catcttcgtc taggacacgc atgtcaagac caagagattg caattctttg acaagaactc
    22261 ggaaggattc tggaacacct ggttttggaa ttggtttgcc ttttgtaata gcttcatagg
    22321 ctttcaaacg tccgttgata tcgtccgact tgtaagtcaa gatttcttga aggacatttg
    22381 acgcaccgta ggcttcaaga gcccaaacct ccatctcacc gaaacgttgt ccaccaaact
    22441 gagctttacc tccgagtggt tgttgggtaa cagttgagta aggtccgact gaacgcgcat
    22501 gcaatttatc gtcaaccatg tggtggagtt tgatcatgta catgactcca acagaaacac
    22561 ggttatcaaa tggttcacct gtacgtccat cgtaaaggat tgtcttggca tcgctatcca
    22621 tacctgcttc tttaacagtt gaccaaagat cttcagaact tgctccatca aagactggtg
    22681 tcgcaatgtg aataccaaga gtacgagctg ccataccaag gtgaagctcc ataacctgac
    22741 cgatattcat acgtgatggc accccaagtg ggttcaacat gatgtcgact ggagttccgt
    22801 ctggaaggta aggcatgtct tctacaggaa cgatacgaga gacaacccct ttgtttccgt
    22861 gacgtccggc cattttatct ccgaccttaa tcttacgttt ttgagcgatg taaacacgaa
    22921 ccaacatgtt aacacctgat tgcaactcat ctccatttac acgtgtaaag atcttaacat
    22981 cacgaacgac accatcggca ccgtgtggta cacgaagaga agtatcacgc acttcacgag
    23041 acttgtctcc aaagatagcg tgcaagagac gttcttcagc tgaaagatct ttctcaccct
    23101 taggtgttac tttacctaca agaatatcac cttctttaac ctcagcacca atacggataa
    23161 tccccatttc gtcaaggtct ttgagggcat cttcaccaac gtttggaatt tcgcgagtga
    23221 tttcttcagg cccaagcttt gtatcgcgcg tttctgattc gtattcttca aggtgaacag
    23281 atgtgtagac atcgtccttc accaagcgtt cgctcatgat aacggcatcc tcgaagttgt
    23341 aaccttccca agtcatgtag gcaacgattg ggttttgtcc aagcgccatt tctccatttt
    23401 ccatagaagg tccgtcagcg atgaaatcgc ctttttcaac gacatcacca acttttacga
    23461 gagtgcgttg gttgtaagca gtacctgagt ttgaacgacg gaatttttgg atgtggtaaa
    23521 catccaatga accatcttca cgacgaactt ctaccttatc agcatctgcg taagtaactt
    23581 taccatcata ctgagcaatc acagccgcac cagaatcgtg ggctgcttgg tattccatac
    23641 cagtaccaac gtaaggtgcc tgaggattaa tcaatggcac agcctgacgt tgcatattgg
    23701 ctcccatgag ggcacggttg gagtcatcgt tttccaagaa aggaatacat gctgtcgcaa
    23761 cggcaactac ctgttttggt gaaacgtcca tgtagtcaac aatattagct ggatactctt
    23821 ggttgacccc ttggtgacgt cccatgacaa tcttctcagc aaaggttcca tcttcattca
    23881 gacgagagtt agcctgagct acagtatatt catcttcttc atcagctgtc aaccaaacaa
    23941 tttcgttcgt gacaacacct gtttcacggt caaccttacg gtatggtgtt tgaacaaaac
    24001 catatttgtt caagtgtccg taagatgaca agttattgat caaaccgatg ttaggtcctt
    24061 caggtgtctc gattggacac atacgaccat agtgagtgta gtgcacgtca cgcacttcat
    24121 atccggcacg gtcacgagtc aaaccaccag gtcctaaggc tgacaaacgg cgtttgtgag
    24181 acaactcaga aagcgggttg tgttggtcca tgaactgtga caactgtgat gaaccaaaga
    24241 attctttaac tgcagctgtt acaggacgaa tattgataat ttgttgtggt gtcaagactt
    24301 cattgtcctg aacagacata cgttcacgga cattacgttc catacgagaa agtcccaaac
    24361 gtacttggtt ggcaagcaat tcaccaaccg cacggatacg acgatttcca aggtggtcga
    24421 tatcatctac acggccaagt ccttcagcca agttgaggaa gtagctcatc tcagcaagga
    24481 tatctgcagg agtcaccgta cgaaccttgt catctgggtt agcattacca atgatcgtta
    24541 cgacgcgatc tggatcagtt ggagcaacaa ccttgaattt ttgaagaaca acaggctcag
    24601 tcacaacggc tgcatcgttt gggatgtaga caatcttgtt caagtcgcca tccaaatggc
    24661 tttcaatgct ttcaatcacg ctacgagtca taatcgtacc agcttctacc aagatttctc
    24721 cagtttcagg gtctaccaat ggctctgcaa tggtttggtt gagcaaacgt gttttaacat
    24781 tgagtttttt attgattttg taacgaccaa ctgctgccaa gtcatagcga cgtgggtcaa
    24841 agaagcgagc cacaagcaag ctacgtgagc tttcagccgt cttaggctca cctggacgaa
    24901 ggcgttcgta aatttctttc aaggcttcgt ctgtacgaga gtccattgga ttcttgtgga
    24961 tatctttttc aacagtgttg cgaaccaatt cgctgtcacc aaaaatatca aagatttcat
    25021 catcacctga gaaaccaaga gcacgaacca aggttgtaaa tggaatctta cgagtacggt
    25081 cgatacgagt gtaggtgata tcttttgagt cgctttcaag ttccaaccaa gctccacggt
    25141 tagggataac agttgaacca tagcccacct taccattttt gtctactttg tcgttaaagt
    25201 aaacacctgg tgagcggacc aactgagaaa cgataatacg ttcaccacca ttgatgatga
    25261 aagtacccat ttctgtcatg attgggaaat caccaaagaa aacttcttgg gtcttgattt
    25321 cgcctgtttc tttattgatc aagcggaagg ttacaaaaat tggtgctgag tagctagcat
    25381 cgtgaatacg agcttcttct agcgtgtatt ttggttcctt gatttcatat ccaacaaatt
    25441 ccaactccat tgtgtctatg aagtttgaaa ttggcaatac atcttcaaac acttccttaa
    25501 gaccgtggtc taggaaagct ttgaatgagt cagtttgaat ttcaatcaaa tttggtaagt
    25561 caagaacttc tttgattctt gaaaaactac gacgggtacg atgtttcccg tattgaacgt
    25621 catgtcctgc caagatgatt ctcctttgta aataagttcc aagccttgtc aatcaggctt
    25681 ttctaatcgt catatggttg taaacccctt atcaccgtgt cctcttgacg atttttcaga
    25741 atctttaagc ctctgttaca aatgctcaaa atcttgaaaa aaagcacaaa aagagcagct
    25801 aaatctgact ttttcagaag atttaactgc tgtgagcctt gtctggacaa tatttcagac
    25861 aaaacctacg acaaatgatt acccatatta taccctattt agctagattt ttcaaggggt
    25921 ttcagtaggt ttttggtaaa ttttttccca tagaaaactt ggcatcacat tcgaatcacg
    25981 ctatggtaca aaaaactgaa aaaactattg actgaaaatc attttcaagg tataataata
    26041 aacgttaagg cggtatagcc aagtggtaag gcacggctct gcaaaagctt gatcgtcggt
    26101 tcaaatccgt ctaccgcctt ctataacttg atttatcagg tttcaaatga acagaaagcc
    26161 caatttgaag ggcttttctt attttccctc gaataaatac gtataacttt aaaaactttt
    26221 ggagcgagtt tgtggcagag ttctttccat ggcataattc ccttttgaaa tcaattgaaa
    26281 aaagcttcgt cacgcctggt gacaaaagcc tctagtttat tctgtttctt cttctatttc
    26341 gacttctgtg atttggattt ttaccttggt aacacgtcca ttcttcactt tatcattggt
    26401 taagataaac tgtttgtttt ggctgaccaa ttcatagctg agtttctcag tcgttggaat
    26461 ggttcccact cctgtcaaat aataaccagc gatagtatca acgtcatcac tttccagttc
    26521 aacattaaag taattattga aatcattgag ggtcatagtt ccttgaacaa tgtaggtatc
    26581 ctcgccaatc tgatgaacat ctattgctgc tttgtccgtt tcatcatcaa tttcaccaac
    26641 aatttcctct aagaggtctt ccaaagtcac caaaccagcc ataccaccgt attcatccag
    26701 caagattgcc atttgtcttt gggtatttcg cagttctttt agcaagtcat ccacaaaaat
    26761 agtttcaggt acaaaaagtg gatcttgtaa aattctcttc caaacaatat tgtcaaaacc
    26821 gtccacaaag cctgccttaa ggagactctt ggtgtgaatg attccaatta cattgtcctt
    26881 atccccatca taaaccggga tacgagaata attttgtttt aaaatacttt ggataatggc
    26941 ttgactatca tcctgaatat ccaccataaa ggcatccgtt cgaggaacca taacctctcg
    27001 tgccatcagt tcatcgagcg aaaagacacc ttgtagcatc tcaatctcat cagcatccaa
    27061 tgtttcttca ctatttgtca gcatataggc aatttcatca cgggtcatct tttcatccgc
    27121 atcatcgaat gacataggag tcaaatggct caagaaattg gtcgaagcag ctaaaagcca
    27181 aacaaaagga ctgactagtt ttccgatccc aatgataacc ggcgctgtac gaattgccaa
    27241 ggcatccttt agattaagag cgattctctt aggatataat tccccaaaaa cgatggaaat
    27301 ataggtcaaa aatgccaagg atagaaaagt tgccacggct tgtgctgttt cgccattccc
    27361 aagccaagag gcaatcacac gtcctagagt attagttaaa ctcgcccctg ataagattgt
    27421 aatcagggtg attcctacct gaatggttga taaaaagtgg ttaggatttt ctagtacctt
    27481 cagcaggcgg atgtagcgtc tgtctccttc ttccgccttt tgttcaactc gggcacgatt
    27541 aagagaaacc atggccattt ctgtggctga gaaaaaggca tttaataccg tcaagataaa
    27601 caataaaaca aattgtagca acaaattctg actacttggg tcttccatag ttcttctcct
    27661 aaaatagtat cttatccttt attatatcac aaaatttaat ccaatacata ttattttttt
    27721 cttagcgtaa cttccatctc tctcccaaaa ctggaagtta gtctaagacg gatttccact
    27781 gatttcacgc gtttttctca tactaaaagc taggttgtgg gaaactggaa gttagtttta
    27841 gaagttacta ttttttctta gtacctgttc tagccctgct taaactagta aaaagaactc
    27901 ctacatccat aagtgacata ggagtttagg cttatctttt actgagttac agttacttct
    27961 ccagttcggt aatccagttg aagtcccttt tgaacattgg gaactagaac attgaattcc
    28021 aattctccat ccgaagactt ggcttcaatc tgtgaagctg agggaactaa atcctcgttt
    28081 gaagcgtagt aaagggttac acggtaacgg acacgcttgt tttggtccaa ggctttacgc
    28141 accttgcttt catagtagtt ttgaccagtc gaatactcgg cttgtgcctg atttgcccag
    28201 gctgtctgaa cagcaatgtt tttaggattg cttgttgagg catcaaaacc atccaaacca
    28261 ccgattaagg catagcctaa caaatgacct ctatcgactg catgggtata agagcccttt
    28321 agattcttga cctgatgcca acctggagga gtccaagaag ttgaaccatt cccagtttct
    28381 ttacgattct tgtactgacg agtggcctta gacaagagag cattagctac ggttggaaca
    28441 gtttccttgc ccactgtctt tgttttattg tcagcgtagg gcttacttga aaccttggca
    28501 tctagatttg ttttattacc attgacgata aaagcacctg agccattcca ctccagactc
    28561 ccctttattt gactcttgac tgcgtctgtt aagacactct ctgccaatgc ttgactagga
    28621 gcttcagacg cttgtttttt ctgactcatt tttgtcttag cagcctgatt tcctagttca
    28681 ttctgcttaa tataatagct ccctgcagac aaaagcaata acactagcag tccaatcagt
    28741 gtctgtcttg tttttttgtt catatttctc cttatcttta taaaaaggct ggtctcccag
    28801 cctaatttcc tgtaaattta tgaatcaatt cctgccattt tgccggagac aggatagccc
    28861 acggatcttg acccttgccc aagattccat agcctaccat taaaccgatt cctaaggcta
    28921 gaaggcctaa aatcagtacc agaatgacta aaagtaaacg cttaattacg taacctgatt
    28981 tcttattcat cttcatcact tgcctcaatc cgctgaatct tagcacctag ctgcgccaac
    29041 ttctcatgga aaccgtagta acctctatcc aagtgaacca atttaccgac cacagtttct
    29101 ccctgtgcta ccaaacctgt caaaatcaag gccgcactgg cacgaaggtc agttgaaaga
    29161 acttctgctc cctgcaaagg ctgtccacca acaatacgag ctgtatcacg gataatctca
    29221 gaatgcaagc ccatgcggcg catctcttct aggtgttgga aacgattttc aaaaactgtc
    29281 tccaccatgg ttgattcgcc ttttgcaact gtcatcaagg ctgtaaattg agcctgcata
    29341 tctgttggaa atcctgggtg gggcaaggtt ttcacatgaa cagcttttag attttctagt
    29401 tgagaacgaa cacgaattcc ttcgtcttct tcaattactt caacacccat ttcaagtaac
    29461 ttggcaatca agggacggtt gtgctcccag acagcgtctc gaatcaagac atcaccacca
    29521 gtcatggcag cagctaccat aaaggttcct gcttcgatac ggtcttggac tacattgtga
    29581 gtcgtgccat gaagtttctc aacaccagta atggttatag tctctgtacc agcacctttg
    29641 accttggctc ccatttcatt aaggagaatg gctaagtcaa caatctcagg ctcacgcgca
    29701 gcattctcaa tcactgtcac cccatcagcc agagtcgctg ccatcatcaa gttctgcgtt
    29761 gcaccaacac ttggaaagtc catatagata tgagcgccat gcaagcgttc tgccttggct
    29821 tcgatgtaac cagctgtctg actaatctta acccccatag cttccagacc tttcaaatga
    29881 agatcaatag gacggctacc aatcgtacaa ccacctggca tggatacctt ggcatgaccc
    29941 acacgggcaa ggattggccc taatacaacg atggaggcgc gcatcttgct gacatacttg
    30001 taaggggctt cctcagtgat gtcgccagta gcatccacct tgacaagatg agcttcctca
    30061 tcaaagtcaa ccttggcatt caaaccacca actacctgat tcataataaa gacatccgac
    30121 aaaatcggaa cattctgcaa gacggtcttt ccttcacttg ctagaatagt cgctgccaac
    30181 aagggtaaga ctgcattttt tgctccctcg atcgtcacgc ttcctaccag acgattatcg
    30241 ccaccttgaa ccacaatttt atccatactc gtttccttta ctcttgattt cataataatc
    30301 ctctaagcgc ttcttgccac aaatgataca ggctaataaa gaaagaactc aggatgtagc
    30361 ctatgacaat gctaaaaaag gctactaata aacgaacttt tcttgtatta tcatctgtca
    30421 cttttaaaac cttctcccat ctaacaagat cctttaaaag gtaaaaactc acataaataa
    30481 agaacatatg actacttaga gtgaataata attgaaccat ttgactatta taccatcttc
    30541 tctgtttgtg cgctagtttg gaaacttcac tcaaaaatta gggattgttt ttcaagtaat
    30601 caatcgcatc ttgtagggtt ttaacgggca cgattttcat atccgtcttg attgttttag
    30661 ccgcttctag ggctgtttgg tagttgtttt tcgcgtccgg atgcgccttt tgttcttctt
    30721 cgctaacagg gttatcagga gcaaagaaaa tagcagcacc tgccctagcc gaagctacaa
    30781 ccttcttatc aatacctcca atgtctccca cattaccatc gcggtcaatg gtacctgtac
    30841 cggcaacaat acgaccatta cgaagatctg ggtgagctat ttgagtatag atagctagac
    30901 taaacatgag accagcactt ggaccgccaa taccagctgt tgaaaagcta attgggacat
    30961 tgctgattac ctctgtacgg tcaatcaagc cgattccaat tccatttttg ccattttcca
    31021 aggtgatgat ttttccttct gcagacttgg tttgcccatc ctcttcatag gtgaccttga
    31081 cggaatcccc taatttttga gaactgacgt aatcaatcaa gtctttggaa ctatcaaagg
    31141 tctgatcatt gactgctgtg accgtatcag agatattgag aatcccttta aaggttgaat
    31201 tatccgtcac attcaaaaca taaactccaa agtacttgag ttcgatatcc ttaccagctg
    31261 tttttagtcc ttgatacttg gccatatttt gcgatgtttg catgtagaat tgattgattc
    31321 gcataaattc aacatcggaa gaaccacctg tagtctcctg agcactacga atatctgtaa
    31381 aaggtgtcaa ccaagcataa atcatatgag ctaaagtggc atgttgaaca ccaaccgtaa
    31441 cgaattgata ggcaccagct tccttatctt ctgtgtcatt tactttaagg acttggcgaa
    31501 tatcttccga accacctgga acctctatat aataaggcaa gggcactaca aatgccaaga
    31561 aagtcacaat caaggccgca atgacgtata agggccatct aatctttttt ttcatttctt
    31621 atttcctcca aaatactctc gggaacatag caggcaatat cctgaccaaa cttcaaaagc
    31681 tctctaacgc ctgatgaact gatatagaga tgttcaggtc gactatgtaa ataaatagtc
    31741 tctatatcag aagacagctg atgattgtag taatcaaaac tggcttcata ttgcaaatcc
    31801 gacgcattcc tcaaaccacg cactagacaa gtagcaccca atctttttgc aacatcgacc
    31861 accaattcat catgagaagc cacgacttca acattttcca aatgtcccaa agccttttct
    31921 agcccccgtt tacgattttc aagagggaga aatccttgtt tgtggggatt aaaaaaaata
    31981 cccacacaaa gcttatcaaa aagtctgctc gcccgttcaa tgatatccag atgcccattt
    32041 gtcatcggat caaatgagcc tgtgaataag ccaatcttat ctgacataga ctgtcacctt
    32101 actaattcca taaatctttt ccttccagat acccagacag gcaatttctt ctggaagttc
    32161 aacggcctta tccgtctcgc acacaaccat aacatcttca gaaaaaagct ctctctcagc
    32221 cattttttca atatctgcta cgatttgttc cttggcatag ggagggtcta agaaaacgag
    32281 gtcaaattcc ccagatacct gttccaatgc cctctctgca tccatcttga ggagttgaaa
    32341 ttttccaact tccttggtca tctggatatt ttcagccacg atggtctgag ccttacggtc
    32401 tcgctccacc aaaacagcac tggacatgcc acgcgatact gcttcgatag ataaaccacc
    32461 actacctgca taaaggtcca agactcgtcc cccttcaaag tagggaccaa tcatgttaaa
    32521 aatggctccc ctaaccttat ccgaagtagg tcttgttgtc ttgccttcta gtgtcttgag
    32581 gggacgtccc ccatagattc ctgatacgat tttcatactg tttattatac caaattatag
    32641 acaaaaagag aaagaaaacc gaaccttgcg gttcgattct ctacaaaata ttttcgtaag
    32701 tatcgcggac ttcttgaggc caaacacttg tttgcacttc tccgatgtgt ctcttgcgaa
    32761 gtaggaacat ggccatacga gattgtccaa ttcctccacc gattgtcaat gggaatagac
    32821 cattcaacaa agacttgtgc cattccaatt ccaagcggtc ttcatcacct gtaatttcca
    32881 cctgacgtct aagagtttct tcatctacac gaattcccat agaagacaac tcaaaggctc
    32941 cacctaaaga ctcattccag acaagaatat caccatttag acccttgtag ccattctcag
    33001 actcgcttgt ccagtcatca tagtctggtg cacgtccatc gtgcggttta ccatctggca
    33061 actcgccacc gataccaatc aaaaagacgg ctccaaattc tttacaaatc gcattttcac
    33121 gttctttcgg tgtcaagtct gggtagcgtt ctaccaattc ttctgtatgg ataaaggtga
    33181 tttgttttgg caagatagac tcgatgtcat agcgggcttc aacagctagc tcagtcaggc
    33241 gaatagcctt ataaatcttc tcaactgttt cttttagata aacgatgtta cgcttaccat
    33301 ttgggataac cttctcccag tcccactggt caacataaac agagtgggtt gcatccaagg
    33361 aatcctcatc tggacgaagg gctttcatgt ggacaaagag accctctcct tcaccaaagc
    33421 caaaacgagc caaggtgtgg cgtttccatt tagcaagtga gtgcaccact tcataagtag
    33481 catcagggat ttggagaacc ttgaccgata ctggattttc cacaccagac aggttgtcct
    33541 gcattccgtc accgacctta ctcaagatag gaccttgaac ttcgacaact tctagcttat
    33601 ctttcaaata ctgggtaaaa gtgtttttga caaaggaaat ttcttcttgt tgatggataa
    33661 aacttttctt catagacaac tcctcaaaaa tttaataaac gtattataca cctatttcca
    33721 agaaaagaaa agagaaaatt taaaaaaatc agtgtcactc aaaacactga tttcatagac
    33781 cttttagtca ttgcgaccaa agatacgtag aatacttagg aaaagattga taaaatcaag
    33841 atagatgctg agagccattg acaccaccca gcctgtcgct acacgacctt gtgattgttc
    33901 ataggcgagg cgaatctttt ggttatccca agcaatcaac cctgaaaaaa ccaaaaccat
    33961 ggctacgcta atcatataat caaagaagcc actagccaag aaaatattga ctaccatagc
    34021 aatcagtaaa ccaatgagag ctgccatcat tgctcgacca atcccactca agtctttctt
    34081 ggtaaagata ccaactgccg ccatgacaaa gaagagaagg gcgctcgata caaaggcaga
    34141 taaaactgta cccggagtat agaaagccac cacaaaacta agggtaaagc cgtttaaaac
    34201 ggagtaaagt aaaaatactg gaagagcagc tggactattt cttgaagcca tgctactggc
    34261 aacgaaaacc aaagccaact ccgcaaaagt agcaatggtc aaccagagac gcccctgcat
    34321 caaaaagtaa accaactgag actgaaagac cgtcaacata aggcctgata ccaaagcaga
    34381 tagtccgatt cccagaccaa caaaggcata aaccttagcg taaaattgat tgagacctgc
    34441 acggtcatgt ataatagtgt gattcatcca tttctccttt tctgtgaaca tctttccttg
    34501 attataacaa aaaaagttaa aattagctta aatggaagat taaaataccg gctgcaaccg
    34561 caacattcaa actctcggcc tgccccttca tgctaatatg gaccaactgg tctgcacttt
    34621 cagccataag gggactaatt ccttgaccct catttcccat aactagtaca aaattttcta
    34681 tagaaggcag ttctctgtaa tcaacagaat ctttagatag ggttgttgct agcactggga
    34741 tagctacctt tttagtttcg tcaagaagcg cttgactagt catccggtaa atgggcagat
    34801 ggaaatgact accttgcatg gaacgtaggg ttttgagact gtagatgtct gccgacttat
    34861 ctgaaacaat cactcctgta aaacctgctg catccgcagt tcgaatgata gttcctacat
    34921 taccaggatc ttgcacatct tccaaaaaca agaacttgcc ctgactcaaa tcagcttgtc
    34981 ctacttcttc tctttgaacc acggcaacaa ttccctgtgg agtctgagaa tccgccaaat
    35041 ctagcaaaat atcctctgac acccagacag tttgcgaaaa agtagctaac tgatctcgat
    35101 aattttctag ggtaaagatt ttctcaatcg tcactccagc ttgaacagct tcttcaaaca
    35161 agtgccagcc ttcaatcaaa taggctgact tgcggtattt tttttggtgt aatttcttgg
    35221 tattttttac cacagaattg gcttttgagg ttataatagt catagaaata ttataacaca
    35281 atcaaagggg tttggtatgc aaaaggttag aatgattgcc caaggtaggg tacagggagt
    35341 cggctttcgt tggggtgttt acagcttggc acttgaaatt ggtggcatca caggtcgagt
    35401 atggaataac gacgatggca cagtggaaat cttagcccaa gcagactcat ctgctatcat
    35461 ggcaaaattt atccaagaaa tccgaaaagg accgacacct ttttcaaaag taagctactt
    35521 agatgtcaaa ctaagcaact ttcctcccta ctctgacttt aaaatcgcaa attaggtctc
    35581 cagaactatt gtatattttg taaaaaaaca gtagaataga aaggtataat ttttaaagaa
    35641 ggaataaaaa cagtgaaatc tattaaacgt tttgcattct cggctatggg agtggctatg
    35701 ttgcttgtct tgactggctg tgtcaatgtc gataaaacca caggtcagcc aacaggattt
    35761 atttggaata cgatcggagc gcctatggct gaagccatca agtacttcgc tactgataaa
    35821 ggtctaggct ttggtgtcgc tatcattatc gtaaccatta tcgtacgctt gattatctta
    35881 ccacttggta tctaccaatc atggaaggca acgcttcact ctgaaaagat gaacgctctc
    35941 aagcatgtcc ttgagccaca ccaaacacgt ctcaaagaag caactactca agaagaaaaa
    36001 ctcgaagccc aacaagccct ctttgccgct caaaaagagc acggcatcag catgtttggt
    36061 ggtgtaggat gtttccctat cctccttcaa atgcctttct tctctgctat ctactttgct
    36121 gcccaacata ctgaaggggt tgctcaagca agctacctag gcattcctct aggttctcca
    36181 agtatgattt tggttgcctg tgctggtgtc ctttactatc ttcaatcgct cctttcactt
    36241 cacggagtag aagatgaaat gcaaagagaa caaatcaaga aaatgattta catgagccca
    36301 ctcatgatcg tcgtcttctc cctcttctca ccagctagtg tcacacttta ctgggttgtc
    36361 ggtggtttca tgatgattct ccaacagttt atcgtcaact atatcgttcg tccaaaactt
    36421 cgcaaaaaag tccgtgaaga actagccaag aacccaccaa aagcaagtgc tttctctaaa
    36481 ccaagtggac gaaaagacgt tacccctgaa caaccaactg ctatcacaag caagaaaaaa
    36541 cacaaaaatc gcaacgctgg aaaacaacgt tcgagataag aagaaaaagg cttagctgat
    36601 ttgctaagtc tttttgcaaa tcaaaagccc gatgtttcca tcaggcttct tttctatcca
    36661 tgtacacgtt tcatataatc ttgataactt tcggtatcca tgagtttttt agcgttcttg
    36721 acgcgatctg ctgttggtgg tttgactcct tcgagggaat atggaattcc aagttcacgc
    36781 cacttgaact cacccatggt gtgataaggt agaatttcaa acttatcaac atttttgagg
    36841 gtcttgacga acttaccaag ttcaatcaag tcatcatctc tgtctgtcaa tcctggaact
    36901 agcacgtggc gaatccagac aggttttcca atatctgata gatactgggc acaagccaag
    36961 atatttttat tagtttgtct agtgacaatc ttgtgctgtt cttcgttgat ttccttgata
    37021 tccaaaagaa ccaagtcagt gacagccatg agtttgtcaa acttctcaag gtaacgtggt
    37081 ttattacgga aaggaagagc acaggtatcc aaggtacagt ggattccttg ttccttagcc
    37141 ttggtgaaga gagcaatcag gaaatcaatc tgcaagagag cttctcctcc actgactgta
    37201 atcccaccct tatttcccca gaaaccacgg tagcgcaagg cctctgtcaa gacatcatct
    37261 accgtccgtt cacgtgactt attggactcc atagcccaag tgtctgggtt gtggcaatac
    37321 tggcaacgca tgtgacagcc ctgcaaaaag acaataaagc gaataccagg cccatctact
    37381 gacccaaagc tttctgtcga atgcaccatt cctgtcactt gtccataatc aattgtttct
    37441 tcagacatat cgttaccttc cttgaaaacg ttttatagtt ttattatatc acgacttgaa
    37501 ggaaaaacaa gatttttgcc tattttttag aaagcaatct gtttttcaat ttcattttca
    37561 atctattatc agattattct tcaaaatcga agccatataa ggttgcaaaa tagtcctcag
    37621 ggcgctctgc acggcggatt tgacgggctt taccttcttc ggtatagagg atttccgcag
    37681 aacgtaattt ggcattatac tggtagccca ttgaaaatcc gtgggcacct gtatcatgaa
    37741 ttaccagcaa atcaccgatt tctgtatgag gcagttcgcg attgactgca aatttatcat
    37801 tgttttcaca gagtgaacca accacatcta ccgcttcagc tggtccatct ggatgggtca
    37861 cgttgctaat atgatggtaa gctccgtaca tagctggacg catgaggttg actgctgagg
    37921 catccacacc tagataggta cggtaggttt ccttcttatg agtcactctt gtgactagag
    37981 caccgtgagg tgccagcata aaacgaccca attcggtgaa aatcttgacc tgaccaagac
    38041 ctgctgacgt aagaacttct tcatacacct tacgaactcc ctcaccaatc aaggcgatat
    38101 cgttcggctc ctggtctgga cgataattaa caccaatacc gccagaaaga ttgataaagt
    38161 ctagccaaat gcccaacttt tccttgattt caacagccag ttcaaagagc tgacgagcca
    38221 actctggata atagagatgg gtcacggtat tggacgctag gaaggagtga atcccaaaag
    38281 tcttggctcc tttttccttc aagatagcaa aggcttcaaa gagctggtcc ttggtcatgc
    38341 caaacttagc ctccccagga ttgtccataa tgtctgtccc cagttcaaaa acgcctccag
    38401 gattataacg acaagagatg atttctggaa tgcctgctac tctctccaga tgttcaatat
    38461 cttcaaaggc atccaagtta atggtcgcac ccaattcacg tgcataggcg tattccttgt
    38521 ctggcgtgtt gttggaagag aacataatct cagaacccag aaagtccagt ttatggctca
    38581 tcaaaagctc tacataacta gagcagtcca caccacaacc ttcttcttgg agaattttca
    38641 aaatagctgg agttggagta gccttaactg caaaatattc cttaaagccc ttgttccacg
    38701 aaaaagcttg gttgacggct cttgccttct cacgaatccc cttctcatca tacaagtgaa
    38761 agggagtcgg gaactcggca acaatcgctt ctaactcttc tctattgata aatggtgttt
    38821 tcataagctt ccttctattc tgtttgcaaa gaaaggctgg gacaatcgtt ccaaccttta
    38881 gttctatctc ttatttatct tcgtcaaaga tattgccaac ctcaacatcg atggcttggt
    38941 tcttgacatc aatattggtt accttcaaga gtgacttgta gtcaaactgc ttttcacgtt
    39001 cttcttgggc attgtccgca aagaccgcta cacctgccag ttctgagtcg aactcgcgca
    39061 agagactaat cataccattg accgttccgc cacctttcaa gaagtcatcc acaatcaaga
    39121 cacggctgcc tgccttaaga ctacgttttg aaaggaacat tttctcgata cggtcaccac
    39181 ttgaacctga aacatagttg acgctaacag ttgaaccttc ggtaattttc aggtcacggc
    39241 gcacaatgac aaaagagaca ttgaggacat tggcaactgc atttgcaagt ggcacaccct
    39301 tagttgctac ggtcataacc gcgtcaattt tttggtccat aaagcttttg gcaataatac
    39361 gaccaatatt tttcaagatg gctggtgtgc taagcaaatc agacagatag atataaccac
    39421 ctggcaagat acggtcactt tctgacaact tggtacgcaa gtcttcaacc atttccttag
    39481 catcctgact cgaaatagac ggtgtgaaga tgacacctcc gccagcccca gtcactgtct
    39541 ggatatgacc gatttcaatt tcctcaaagg cgcgtttgat aatgacgata tcttctgaga
    39601 tggatgattt agcagactca tacttttcag caaaagtatt gagactagtt agtttataag
    39661 gattattaat caaatagttg gaaatgacaa ccatccgatc acttcttctt aatttcattt
    39721 ctatttccta ttccattttt attcaaaaaa tcaaaaagca aactagaaag ctggtcgctg
    39781 gtggttcaaa acactgtttt gagattgtca atagaactga caaaccctgt aatatacctg
    39841 catatataca tacgacaagg cgatactacc ctagtttgaa gagattttcg aagagtattc
    39901 atttttgtct tttacttatt ataccatatt cacataaaaa aacgaacatt cttatcctaa
    39961 aaaatgctca tttttcttaa attatcaatc taaatctggt ttatagaagg aacgattatc
    40021 catagcgaag attttattgg tcatctctcc tttatccacc aaagccagag ctgttgacat
    40081 catcatcatg cttgcatcca gattgtcaat catatggata atctctgcct ccataatacg
    40141 tggacggact ggacttccat attcaagcaa gccgtggtga ctgaggatga catgacgaag
    40201 caaaacgact tcttccttgg tatcatcgat gccgagttcc ataactgtct tggtaatttc
    40261 gctatcaatg agagcgatat gtccaagaag attacctcgc actgtgtact ctgtctggtc
    40321 tggccccgtc aactcgatga ccttagccaa gtcatgcagc ataatccccg catagagcag
    40381 gctcttattg agctgaggat aaacttcgct aatagcgtct gccaaacgca ccatggtcgc
    40441 cgtatgatag gccaagcccg tttcaaaggc atggtggttg gtcttggcag ctggatagga
    40501 gtagaattcc ttatcatact tggtgtagag atttcggaca atccgttgcc agacaggatt
    40561 ttcaattttg aaaatcattt gcgacatgta gtcacgaatt tccttgacat caactggtga
    40621 cttgaccttg aaatcagctg ggtcattggg ttcaccagct tgaggcaggc ggagagtaat
    40681 ttgattgact tgaggggtat tgttataaac ttctcggcgt cctttcatgt gggcaacctt
    40741 acctgcggta aaggcctcaa tgttatgagg ttgggcatcc cagagcttcc catcaatctc
    40801 gccactatca tcttggaagg taaaggctag gtagtttttc ccagctcgag tttgcctcag
    40861 gtcagctgat ttgattaggt aaaagccttc aaataactca tcttttttca tgtgactaat
    40921 cttcatattc ttcctcattt tcttgaaaat ggagtagatc aagcgcaggc tcaccttctg
    40981 acaactcaat gtgacggagc gtccgctcga tagctatggt acgacggttt aataattcat
    41041 caatattgcc agaggcatgt tggagatgtt tttgtgcctt gaccagaata ccaccaaact
    41101 tgccaaactc ggtcttgaca ctggcaagag tcttgctgat atggtcggca ctcttttgga
    41161 tattaagggt cttgaaacca actgataggg agttaagaag ggctgatagg gtacttggtc
    41221 ctgcaacaat aatctgttct tcccgtctca aatcatcaaa gaagaccgga ttgcggacga
    41281 tttctgagta gagaccttct gtcggaacaa acaaaactcc aaaattggtc gtccgaggtg
    41341 gtgctatgta cttgttccta atatccctag caaagcgctt gacgcttgct aggagtgact
    41401 tacgacagcg ttcaatctca tccttgtcac ctgtctcata ggctgcttcc aagcggtaat
    41461 aatctgccag tggaaactta gagtcaattg gcagatagac gtattcttgg tcgccttgtc
    41521 cgggtaactt gatggcatac tccactcgtt cactagagtt ttcaaccgtt gcgtattctc
    41581 gttcgtactg ggcaggtgtc atgatgtctt caataatttg ccccagttgc aattctccca
    41641 gaatccctcg cgtcttggtt ccagagagaa ccttgttaag agctccgaca tcacgggcaa
    41701 ctgtctgcat ttctccaagg ccacgattga cagactccag ttgtttagaa actgtctcaa
    41761 aggaagcctg taagcgtgtc tgcaaggtct tttctagttt ttcctcgacc gtctggcgca
    41821 tttgttccaa acgttgctca tttgattcct gcaaggcttg gagacgttgg tctgtcttgt
    41881 ctctagtttg gaggagatta tctgtcattt cttgacggac ttgggtcaga ccttggtgca
    41941 attcaatccg cacttcttgc aaacggtcgc tgacaaccac ttccaaatct ttttggtcta
    42001 actggctggc ttgtctggct tggtcaaagc ggtaatccaa ctggtctgac aaatgatctg
    42061 cctgatcctc caagctctta cttaagtgtt tctcctgcct atcctgcctt tgccaaatca
    42121 gaaagagacc agctagattg gcaattaata atagaataag taaactttcc atcctacctc
    42181 ctgtccttgc tatgcagtac gaccacataa ccatctgggc aagttaccga cacttcccta
    42241 tctatatatt cgttagaagc gtacactttt ttaaagaaaa aattttcctc tgtcaactca
    42301 tacttggctc caagaatagt cagctggcta tcccgaactg gcataaaggc tagatagtcg
    42361 tagtctgaac gaggttctag ctgactgatt ccttctggac aataagtaat caagttttgc
    42421 ccatcctcaa tttctatttg atgcatatag ggtgccaact taggattgct aggcagaaag
    42481 acattggcca acatatggtc aatacggcca cccaaggcac cgaaaatagt gacctgagcc
    42541 tgaggatttt gttcaaagat ggttaagaga gccaattcca gatctgtatc atccttttct
    42601 ggtcgtgctt ggacaaaata ctgggcacct ttttgaatca cctgtcgctc ttcttccgtc
    42661 acagaatcaa aatctccgac tgctagagca agaggtaagt cttcttccaa gacccagagc
    42721 gagcctcgat ccaccccaac aaaagcatca aaatctgtcc gataatgacc gcggtttccg
    42781 cctgcaaaaa ctgcaaccct agtccagttg ttttctgaga gtttgtactc gctcattgac
    42841 ttctccctta aagacatagg aacctgctac aaaaacagtc gcaccggctt ctttggcttg
    42901 agcaatagtt tggtcatcaa tcccaccatc cacttcgatt tcaaagttca aacctttttc
    42961 ctcacgaaga gcaaccaact cacggacctt atccatggtt tctggcagaa aggcttgccc
    43021 accaaaacct ggattaaccg tcatgactaa aacttggtca actagatgaa ggacgtgctt
    43081 gatggcttca actgatgtgc caggattgat aacgactgaa ggcttaactc cgagtgaacg
    43141 aattttttgg agggcgccat gaatatgagg cgttgcttct acatggatac tgatgatgtc
    43201 tgcacctgca cgcgcaaaat cttccagatg atgctcaggg tttgacacca tcaagtggca
    43261 atcgaaaacc atcttactat gaggacgaag gctctcgacc acacctgcac caaaactgat
    43321 ttgcggtaca aaatgactgt ccatgatatc gatatgggca tattctgccc cagttgcttc
    43381 tagacgtttg atttcacgtt caaagttggc ataatctgct gccagaattg acggagcaat
    43441 cttgtattga gacataggtt tctccttatt ttggaatttt tttgctgact tttttatagg
    43501 tttctctacg attttcaatt tcactaagga attgcaggta attgtcaaaa cggaaggttg
    43561 caataacacc ctcttcaaca gctggtttga cggcacaaga cggctcatgg gtatgggtac
    43621 aagtacggaa cttacaatct cggctaacag tagcaatctc tgggaaagcc tgattgaggt
    43681 cttcagccct tgatacttca tagtccaagg atgaaaatcc tggtgtatct gcgattttac
    43741 ccccgttgag attgtaaaaa ctaacagctc gagtggtatg gcgaccgcga cctagactgt
    43801 ctgaaatttc tcccgtttca agattgaggt ctggtgcgat tttattgaga agagttgact
    43861 tcccaacacc tgtctgcccc ataaagaccg taaccttgcc tgttaacaaa gacaggagtt
    43921 cctctttact ggtcacaaag tcatagccga tgtcaccata ggtctgctgg taaaaatcca
    43981 gttctcccct atcttccaac aaatccattt tggaaatata gacaatggga tggatgccct
    44041 tgtgctccaa aagaaccaag aaacgatcca gcaaattgct gttaaaatca ggttccttga
    44101 cggacatgat tactacagct tgatcgatat tgacaatagg cggacgaacc agactgtttt
    44161 tccgttcgtg aattttgagg atatagcctt ctgaattttc ctcggcagag aaatctaccc
    44221 agtccccaac ataaggggta tggccttttt tacggaaatt cccacgcgcg cgtgtttgat
    44281 aaacctggcc atcactctcc acatagtaga aacctgccaa ggctttaatg atttgtccct
    44341 gcatcttaga gtccttgccc tttaagtgcg tctgctagac cggcaaattc tgccaagctg
    44401 agagcttccc cacgcacact tggtgacaag cctgcctggt ccaaagcctt ggtcagcttg
    44461 tccttgacct cttcagtctt accaaagtaa cctgtcaagt tattccacaa ggtcttgcgg
    44521 cgatgggtaa aactagcctt ggaaacctta aagaaaaagt tctcatcttc tactgctacg
    44581 gctggctctg gacgacgcac cattttcaag atggctgaat ccacatttgg cgctggcaca
    44641 aagaccgtac gaggcacgat aaaggcaacc ttggctgtca tgtaatactg cacggcgata
    44701 gacaagctac cgtaagcctt ggtgttaggc tgggctgaaa tgcggtccgc tacttctttc
    44761 tgcatcatga ccacaaactc acaaaaagga atgccactct caatcaagtg catgagaata
    44821 ggcgtcgtga tgtagtaagg caaattagcc actaccttga ttggcaggtc aggattttta
    44881 aaattctgga tatgttgcgc caaatcaacc ttgagaatat cttcgttaac tacggtcaca
    44941 ttatcaaaat cacgcagggt atctgccaaa attggcacca aacggtggtc aatctcaaaa
    45001 gccatgactt gggctgcacg ctcagccaaa aattctgtca aggcaccaat acctggcccg
    45061 atttcgatga cattgacctg atcatcaatt tcagccgtat ccacaatttt ttgaaggata
    45121 ttggtatccg tcaaaaaatt ttgcccaaag gactttttaa aggtaaaacc gtgacgctcc
    45181 agcactgcct tggtcacgct ataatctgca attctcatct attctctttt ctattatctg
    45241 ttactactat tgtaacacat tccccttgta ttttgggaat gaaaatcata ccaaaaaact
    45301 atctttcctc atgaagaaag atagtcttgg aaatctttaa aagtggcctt agcagttctc
    45361 tcgaaacatg cgttcaaaaa aatcagcttc ttgataactt cgattttcag gtagggtcaa
    45421 atccacatcc tcctccacat catccactgt cagttcaacc atctgaggta aatttttcaa
    45481 acgataatgc ttttgccttt gcccttcaat actagcaaaa tcaagcaaat agccatgcac
    45541 tcggatttga accaaaagga gatgattggt catattttcc atgatgagga tatcttctaa
    45601 accacctctg gcactaggat aatcagatac cctgcgaata cggatttttt cacacatgag
    45661 caagtttcct catatcaatg atttcatcag ccttattcca gactagggga ttatgggtcg
    45721 caatgatgat aattcgattt tcatctttca aatccaccaa gagattcata acctcttctg
    45781 aattttcagg atcaagagct gctgttggtt catctgccaa aatcaagggt ggatttttca
    45841 aaatagtctt agcaagggca actcgttggg cctctccccc agataaagta tagatttttt
    45901 gttctaaatc caaataccct agattaactt tttctaaagc ccccacttgc ctttccaaac
    45961 gttctacttt tgagattttc tgaccaacaa aacccaaatc caaattttct ttgattgatt
    46021 ggttttctaa gaggccgaaa ttttgaaaga gatagcccat ctggtctcga aaatactcac
    46081 gagtgggaat ggtttttaaa tctttcccct gatagagaac ccttccacca tctatctttt
    46141 ctagctttcc caagatattc agcagcgtcg tctttccgct tccactcttt ccgattaagg
    46201 cataaacctt gcccttctcc agcttgagat tcaaaccaga gaaaatcgcg cggtcattaa
    46261 atttcttttc caatccttga atatcaatca tcttattttc ctttcaatac tgccatgcta
    46321 ccagcttctt cttttttgtc ctgccttact aaaatcaaga gggcgttaag cgtaaacaaa
    46381 gctacaacta gagcgctaat caaaccatct cttgtcaaaa tactagatag gactaggcca
    46441 agcaagagaa ctcctccttg cgccagtaaa tacttgccat gaagctcata gattgtcata
    46501 ccagcaagac gtttaatcat aagttcccgt ctgaactgct caaaatagag aagattcatg
    46561 gaatcaaata acaagatagc cgtagacagg gtcaaaatcg tcccaataag gagagagtaa
    46621 aactccaccc gtttgtcatt tagtaccttg gcaaaaaata gctggctttt taccaagtaa
    46681 gaaaccttgt gatacagacc tttctctttt agagctgtga tgctgtctcc atatcgatct
    46741 agtttcaagg cactattagc cgtagttccc cacaacatcc ctgcaacagg tcttgttcca
    46801 gtcgcttgcg gcgttagaac tacaataatc ggatctttta ggtactggcg tgttgtcttg
    46861 tacccatcat tatagaggaa acgttcctgt cctgtttctg taaaagctag ccttacctgt
    46921 gggaggtagt gtttctgact cgtcacttct acactttcag atgaaaagtt ttgcaggtaa
    46981 tctgtaaaca atccttggta gtagacagac tgctctcgca agctctcagg caagatcagc
    47041 ccaaactctc cctcagacaa gttttgcatc ttgtccataa actctgaaga aacggtaatc
    47101 ttttcttcta tcagatagcg tggtgagaca tagataacat tccctgacgg tgtgtagtca
    47161 ctgagacgat tgccatctag gtccacttct gctccatctg agaaattgtc aacattgctc
    47221 ataatataaa aagagtctgt attggctaac cgttcttcag taaatgtctg ccactcacga
    47281 ttatccttac gcgttccttc ttcatcggca aatgcactag accaaccaaa ggatagacgg
    47341 taacggtctg aggactggct ccatttattg ctagctctct ccatttcctg catttcacgg
    47401 tagtggggta ggagagctgt cgcactcgat ccgaccacca atacagctaa gagttgcccc
    47461 accatcatca atgtcattat acgtttgaga gggagtttcc ctttcaatag atccaccaga
    47521 ctattttcct gtaaaccaag tagatagacg acacttagta aggtagaaat ccctgccagg
    47581 gtcaatccat aaagtagaag agcaatgatg accagttgca ccgttgccat aaacaaggca
    47641 ccttgatacc agagaatccc caatcccaaa agactggata ccagcactga gcagataagc
    47701 tgtctcacat cttctaacac tggtctgaga gcaactccaa acaagctctc accagctatt
    47761 aagcgaatcc ctgcctgacg aagggatttg atccgataaa tcagggtcag actcataaag
    47821 gtcagcagaa aaatagctaa actcagtagc accatagggg tggccgtcaa tagtaagact
    47881 atcgaaaatg gatcttctcc attcgaaaca aagccttgat aaccaagctc tttcaaggtg
    47941 gtctgtaagc tcactccatc caaacttcct gatacaatca agtaagaccc taataaatca
    48001 ctcgtttctg cactctcctt ggaggaaatg gtcagacctt ctggaagctt tccctcacca
    48061 taagtggcat aggtaaagcg tgtttctcca gcttcatttg gctcaacgat tcgacgagca
    48121 atgagactat tgtgctctac cgccaagtga ctcaaagcct ctgtcacctc ttcgcgacta
    48181 gctactggtt gatttatcat cattcctgtc acatcaacag aaggatagtt accaaataaa
    48241 atctcttctc gatggtcaac ggaagttatc accatataaa gcgatactaa taccattgaa
    48301 atcaaaataa ataaacgttt catcatttcc tccaaaaagt tacttggtca cttagtgaga
    48361 aggtgaccaa gtaaagctaa acaatagggc tataccattc taaacattat agtagaatgc
    48421 aactttctct ccccaagaag tacgaatcca cgcacgagaa gtccctccag caccagcata
    48481 tcccttgtca gatgaaccag tttttctgct aacaactgtt gaactatggt attttgaagg
    48541 gtgatagtag tttgagtaag caccaccgtt acctgaacca taagtccaaa caccaccttc
    48601 tgggtattga acggctgcag aggcaactga tacacctcca cctaatgttg ctgcaatgct
    48661 cgctacaaga gctaactttt taactgtttg tttcatattg atactcctct atttttgatt
    48721 acaagattgt tgagttactt gctcctcttt ccattatcaa cgttgatact attatattac
    48781 caaatgatat tctaaaatta tttgtttttc tctaaattgt caaaatagca ccctgaaatg
    48841 aacttttata gtcattatac tccatcttta tcacactatc aaggacatca atgtccttaa
    48901 aatctaacta tttcatatta tttttagctt atatctaaaa aaacagttct aaagacgaac
    48961 tttagaactg ttttgatacg aggacaagga catttttgtc actgattcac tatgtttata
    49021 aatatttttt gacatcctct tgccattcct ctgtcaattt ctttactaga aatccatctc
    49081 caattagctt cgcaaatgtt ttagaagact gataaaatga ttcagctaaa ctaaaatctc
    49141 tttctactct taatgcttgc ttccacaaaa acataaatac aagaggtttc ttttgataat
    49201 cttgagtttt atccataatt tcttgtaaac acgatagata atcattaaaa caatcagtta
    49261 ctcctacctt ttcaagacaa cacattcctg aaataagagt atctctaatt ataaagtaat
    49321 tctctatttg agttaccttc ttctgttcca aaagaaagag cattagcttt tcgatttgat
    49381 ttataaattt ggcaagatct tttctaatga gcatttgata aaaaaacaaa cgaatcaaca
    49441 acatatcatt tattgaataa gcttcttttt ctataatctg aggcatatgt tcctcaagta
    49501 ttaactctgc atattcaggc cttctagttc tagaagtatc caaacacgcc tgcaatactt
    49561 cacatgctaa ttgttcttct ttaggaagat tatcaaagta tgttttataa atctcttcca
    49621 aacacgcttc cttcttatcg tactcctctt ctttaccata gattggttca cgtaaaatct
    49681 ggtatttcaa ttctaaataa gcagaaggaa gagctgaaaa atccggcata aggctataaa
    49741 ctggaactcc tagccggcga gcaatatagg ctaacttggg caaactcggt tgcgaagctc
    49801 cactttcaat tcgcgacagt tgacgaactg tcagttcttg ctcatctcca caaaaatctg
    49861 gacgactaat tcctttttct atccgaatct ctttaatttt ttgaccaatt aacatcagac
    49921 gcctcctctt ctacctgctt cgaaaaatac tgtgaaactt aaaaaattgt aacggctaca
    49981 tctttcctac tcataagatt tcatagcttc ttccacttct gccaaagtaa ccccaaacaa
    50041 ctctaggcgt ttgaggagtt gcttgccgtt ggaatagccg attcggagag tctctccgag
    50101 atattctcta cgcttacggc tgtctgcccc tgctagaaaa ccaaggcgaa tcaagtcgct
    50161 acgactaatg tcaaactgac tctcatgttc aaattgttct gtcacctgag ctagagccgt
    50221 tttcaggtct tcatagctgg catgctcaat tcccagagaa cgccccttgg tcttggactt
    50281 gggaacagct tcatctcgct tgagaaaggc atgctgaact gttggaatgg ccgtcataat
    50341 catgcgccga atccgctccc cattaaaatc tgggtctgta aagacaatga ctccatgacg
    50401 ttggtgtaga cgctgaattc gctctatatc ctgatcattg atggcagaac ctcgtgtctc
    50461 ataggtctcc acatcgaaat aacgcttgag attgaccgta tcatcgcgac cttcaaccac
    50521 gataacttga gaaattttct ctttcattac ttgctgtcca atccaaaaat tcgttctgca
    50581 tttgcagtcg ttgctaccgc cagctcttct gtcgtcatac cacgcaagtc agcgataaag
    50641 tcgaccacat agcgagtata ggctgtttta ttttcacgac cacgcttggg tacaggtgct
    50701 aagtaaggcg catctgtttc caccaaaatc ttgtccaaag gtaactcttt agctgcttct
    50761 tggaggtcag ttgccttctt gaaagtcacc actcctgaga aggaaatggt cataccaaga
    50821 tccacaaact tctctgccca ctcaagcgtc cctgaaaatg aatgcatgat accaccacga
    50881 ggaccaacgc cctcactctt gataatctca taggtatctt ccagcgcatc acgggtatgg
    50941 acaacaaaag gcaaatccaa gtccttagat agctgaatct gacgacgaaa aacctgctcc
    51001 tgcacctctt tgggcgctgt catccaatgg tagtctaagc caatttcacc tagagccaca
    51061 accttgggat gttttaactt atccaacaag taagcctcaa cttcctctgt ataagtacca
    51121 gcttctgtag gatgccaacc aatagtcgca tagagctgct catactcatc taccaactcc
    51181 aaggcacgct caatcgtcgg tttatcaaaa ccaacaatat tcatctgtat cacacccatc
    51241 tcagcagcca aggcaatttc ttctgcctca cgacctgcaa attcttctac attcaagtgt
    51301 gtatgtgtat caaaaatcat ctcttctaac ctcgttttct atcttctatt ataccaaaaa
    51361 agaggaggag cacctaattt ttcggtttcc cctcctctct tcaatagaga gctattctgc
    51421 tatcttttct atccgatatt gcccatctcc tattccacag ttagagacag aagagattgg
    51481 ctacattgat agaatattcc tattcagcta aagtatgtga ttatcttggg ttctcacttt
    51541 tctcacccat cactaggatt gatgacttct caaaatcccc cttataatct aactttaagt
    51601 agagaataga attttctagc tgttctattt tctacgtcta gtctttcttc ctagcaaagc
    51661 aaaaccactt gttgccacca ctagaccaag actagcaagt gctgcacttg atgctgaacc
    51721 tgtgttaggt aattgtttgc cagcaggtga ctggtaggta acatcttgca ttgttcctgc
    51781 agttgcttgc ggtgcacttg ttgtcttagg agcagcatta ggagccacta gtctcttagg
    51841 aactgtaacc atggcaatgg cttgcactcc aatcgtttct gcttccttca catcttccat
    51901 agttttcgca ttttcaatct ctttgagagc tgcttgtttt tctgcttcca ctcttgctaa
    51961 aagttctgct ttttctttat cagaaagcgg tgcgcctttg atttcatcct gcttgtcctt
    52021 ggctgctttt tcaatggcat ttttagctga ttctttttca gtagctaatt gcttagaagc
    52081 ttgtgattga gctgagtttt gtgaacctgt gtgagctgat ccacccgcct ggctaccacc
    52141 gcctgcattc gcattagcat ctgagcttcc agtattgcct cccttatctc cacctggtgt
    52201 attttctagc ttatactctg gtgtagcttc ttgagttaca gattcacctt tcgcagattg
    52261 ttgaactgta tcttttccta gaatcgtcac tactgaacca tctgggaatg tgattgttgc
    52321 tgtaccatct ccagctacat tgattgtcgc tccgtctaat gctgaaccat ttgcttgtaa
    52381 aatagcaacc ttaacttttt cttcatctgt taaatgagtt atatctttta catctactct
    52441 ttgagcaggt gctttaacag ttgatttttc agcagtcggt ttagtgctat cttgtgcttt
    52501 tggatcttgt gctaccaatt gatttcctgg aattgtaata gctgaaccat ctttaaagac
    52561 gattgtcgct gtgccatctc cagctacttc aactttatag taaaatgaaa taataacagg
    52621 acaaatcgat caggacagtc aaatcgattt ctaacaatgt tttagaaata gaggtgtact
    52681 attctagttt caatctacta taacatcgct tccgaatcca tttgcatcta ctgaactttt
    52741 ttacaaaaat aaaaatcagt agactcgagt tcctactgat ttttgataga attcgtttgg
    52801 attatgcagc caaaactttg cgtcctttac gacgacgagc tgccaatacg cgacgaccat
    52861 ttttagttga catacggtta cggaatccgt gtttgcgcgc acgacgaagt ttacttggtt
    52921 gataagtacg tttcacgatg aatacctcct catagatttt gtattcgttt agccggctat
    52981 aaagtgatca gttactaaac atactttact attctatctg attctttcac ttttgtcaat
    53041 agctctaggc aaatgtttcc agctttttcg aatgcaagcc ctatctacga tactgtttca
    53101 agaaacgagt catcttttct tggatttggg ctaactcatc aacacgagga aggtaaacga
    53161 tacggaagtg gtctggttcc tgccagttaa agcctcgacc atgaaccaag agaacctttt
    53221 cctgcttcaa gaaatcaagg acaaactgct catcatcatc gatacggtac atattgcggt
    53281 cgatttttgg gaagatatag agccccgcct tgggtttaac ggcagacaaa cctggaatat
    53341 cttgaatggc attatagatg aaatttcttt gctcgtagat tcgtccacca ggaagaagca
    53401 attcatcgac tgattggtga ccccccaagg aagtttgtac gacttgttgg gccaaaacgt
    53461 tagagcaaag gcgcatattg gacagcatat tgagcccttc gatatagccc ttaacatgag
    53521 tcttagggcc agacaagacc atccaaccca cacggaaacc tgctatgcgg tgggattttg
    53581 acagaccatt catgctgaca cagaagacat ctggtgccaa gctcgccaca ggcgtatgca
    53641 catgtccgtc cattaccatg cggtcataga tttcatccgc aaagataatc aaatcgtttt
    53701 gacgggcaat ctcaataatc tccagcaaga gttccttagg ataaagggct ccagttgggt
    53761 tatttggatt gataaggaca attgccttgg tattggaagt gatttttgac ttgatatcgt
    53821 caatatctgg gtaccattcc acagcttcat cacagatata gtgaacggca tttcctccag
    53881 ctaggctgac agccgccgtc catagaggat agtctggcat tggcactaag acctcatccc
    53941 cattgtccaa aagcccctgc atggacatga caatcaactc actgacacca tttccaaggt
    54001 aaatatcatc aatatctacg tttgggaatt tcttcagctg gcaatactgc atgatggcct
    54061 tacgggctga gaaaatccct ttggagtcag aatagccttc actatcacgc gcattcataa
    54121 tcaagtcatg aatgacctcg tctggcgctg taaagccaaa ttctgctgga tttcctgtat
    54181 tcagacgtaa aatcttttct ccattcgctc gcatccgcat ggcttcttcc aatacaggac
    54241 cacggatatc ataagcaaca tgctctaact tactagactt gttatattct ttcatcttgt
    54301 ttcctcaatt catcaggata gtaacattat accactagaa agagaatgcg acaagtttac
    54361 tgaaatgtgt tactagtttc gtatagaaga aaaaccttgc taaaggcgag gttaagaacg
    54421 tagagatagc aggtattttg caaagacaca tcacatacca aaagataaac ctagaaaaag
    54481 caagcctaga aaggagtcat aaagaagatt aaacatgagg aaaagccccc atatcatcaa
    54541 gtaatcccaa cgcccacttc gttttgcaac tagaaataag attagatggt agagtacatg
    54601 aaagactaaa aatccaataa aacttggata aagaagcggt accagactct ctaattgcca
    54661 gatcatgatc acttctacca agactgaaac taggataatt ccataataaa ggaaatttga
    54721 tttttgaaaa gagtttttca tcattctccc ttccttcact tctttcctgc tatcct
//
LOCUS       DBERDK010000004        50626 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.004, whole genome shotgun sequence.
ACCESSION   DBERDK010000004 DBERDK010000000
VERSION     DBERDK010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 50626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50626
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.004"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(78..1967)
                     /locus_tag="ACL73L_000214"
     CDS             complement(78..1967)
                     /locus_tag="ACL73L_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HGS0771267.1"
                     /translation="MKKTFFLLVLGLFCLLPLSVFAIDFKINSYQGDLYIHADNTAEF
                     RQKIVYQFEEDFKGQIVGLGRAGKMPSGFDIDPHPKVQAAKNGAELADVTSEVTEEAD
                     GYTVRVYNPGQEGDIVEVDLVWNLKNLLFLYDDIAELNWQPLTDSSESIEKFEFHVRG
                     DKGAEKLFFHTGKLFREGTIEKSNLDYTIRLDNLPAKRGVELHAYWSRTDFASARDQG
                     LKGNRLEEFNKIEDSIVREKDQSKQLVTWVLPSILSISLLLSVCFYFIYRRKTTPSVK
                     YAKNHRLYEPPMELEPMVLSEAVYSTSLEEVSPLVKGAGKFTFDQLIQATLLDVIDRG
                     NVSIISEGDAVGLRLVKEDGLSRFERDCLNLAFSGKKEATLSNLFADYKVSDSLYRRA
                     KVSDEKRIQARGLQLKSSFEEVLNQMQEGVRKRVSFWGLPDYYRPLTGLEKTLQVGMG
                     VLTILPLFIGFGLFLYSLDVHGYLYLPLPILGFLGLVLSVFYYWKLRLDNRDGVLNEA
                     GAEVYYLWTSFENMLREIARLDKAELESIVVWNRLLVYATLFGYADKVSHLMKVHQIQ
                     VENPDINLYVAYGWHSMFYHSSAQMSHYASVANTASTYSVSSGSGSSGGGFSGGGGGG
                     SIGAF"
     gene            2105..3670
                     /locus_tag="ACL73L_000215"
     CDS             2105..3670
                     /locus_tag="ACL73L_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HGS0771268.1"
                     /translation="MRKIYLSIFTSLLLMLGLVNVAQADEYLRIGMEAAYAPFNWTQD
                     DDSNGAVKIDGTNQYANGYDVQIAKKIAKDLGKEPLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTAERKQEIAFSSSYYTSEPVLLVKKDSAYASAKSLDDFNGAKITSQQGVYLYD
                     LIAQITGAKKETAMGDFAQMRQALEAGVIDAYVSERPEALTAEAANSKFKMVQVEPGF
                     KTGEEDTAIAIGLRKDDTRISQINASIETISKDDQVALMDRMIKEQPAEATTTEETSS
                     SFFSQVAKILSENWQQLLRGAGITLLISIVGTIIGLIIGLAIGVFRTAPLSENKAIYG
                     LQKLVGWVLNVYIEIFRGTPMIVQSMVIYYGTAQAFGINLDRTLAAIFIVSINTGAYM
                     TEIVRGGILAVDKGQFEAATALGMTHNQTMRKIVLPQVVRNILPATGNEFVINIKDTS
                     VLNVISVVELYFSGNTVATQTYQYFQTFTIIAVIYFVLTFTVTRILRFIERRMDMDTY
                     TTGANQMQTEDLK"
     gene            3670..4434
                     /locus_tag="ACL73L_000216"
     CDS             3670..4434
                     /locus_tag="ACL73L_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGS0771269.1"
                     /translation="MTQAILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IVAQTTVLKRERTEAEEIAKENLEKVGMGERYWQAKPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEVLKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYLILNENQRAN"
     gene            complement(4633..4848)
                     /locus_tag="ACL73L_000217"
     CDS             complement(4633..4848)
                     /locus_tag="ACL73L_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771270.1"
                     /translation="MAKTNFEKVESVVGWVRDKKITGYRISKETNAREMSIIALAQGR
                     AKVKNISFETALGLIDFYEKNYEKFED"
     gene            complement(5718..6968)
                     /gene="ilvA"
                     /locus_tag="ACL73L_000218"
     CDS             complement(5718..6968)
                     /gene="ilvA"
                     /locus_tag="ACL73L_000218"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HGS0771271.1"
                     /translation="MLSSKDIIKAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENA
                     QRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITT
                     PQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
                     VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKA
                     AFEAGGPVKLKEIDKFADGIAVQKVGQLTYEATRQHIQTLVGVDEGLISETLIDLYSK
                     QGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLI
                     RRMEGFDPAYINLNGNETLYNMLV"
     gene            complement(7018..7374)
                     /locus_tag="ACL73L_000219"
     CDS             complement(7018..7374)
                     /locus_tag="ACL73L_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HGS0771272.1"
                     /translation="MNITNLFSIKTGCDETDRQLQKLFFQLDLQLGELTDQLRKLDSN
                     FVPRSQFVDTLDLNDVEYKEILNYFIFHRNDSEESLVEWLYDWISTNRYELPKEFSIR
                     MAHKYHESVTEVFGDE"
     gene            complement(7612..7848)
                     /locus_tag="ACL73L_000220"
     CDS             complement(7612..7848)
                     /locus_tag="ACL73L_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771273.1"
                     /translation="MLADQNKADWSYDENAVINIYDDANFEDGRLHMTFEQFFKLAQI
                     AREEGLEIHSPFERAGATKSARYIAKWILRNKKY"
     gene            complement(7955..8977)
                     /gene="ilvC"
                     /locus_tag="ACL73L_000221"
     CDS             complement(7955..8977)
                     /gene="ilvC"
                     /locus_tag="ACL73L_000221"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HGS0771274.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDTYTVAEATKLADVIMILAPDEIQQELYEAEIAPNLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGPRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            complement(9043..9519)
                     /gene="ilvN"
                     /locus_tag="ACL73L_000222"
     CDS             complement(9043..9519)
                     /gene="ilvN"
                     /locus_tag="ACL73L_000222"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HGS0771275.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGATEDPNVS
                     RITIIIDVASHDEVEQIIKQLNRQIDVIRIRDITDKPHLEREVILVKMSAPAEKRAEI
                     LAIIQPFRATVVDVAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            complement(9512..11212)
                     /locus_tag="ACL73L_000223"
     CDS             complement(9512..11212)
                     /locus_tag="ACL73L_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HGS0771276.1"
                     /translation="MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDTLPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVQFHA
                     "
     gene            complement(11412..13079)
                     /locus_tag="ACL73L_000224"
     CDS             complement(11412..13079)
                     /locus_tag="ACL73L_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HGS0771277.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFRGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDAVEVMRAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFCSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQERMTAALSD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGKILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
     gene            complement(13082..13447)
                     /locus_tag="ACL73L_000225"
     CDS             complement(13082..13447)
                     /locus_tag="ACL73L_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HGS0771278.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            complement(13604..13792)
                     /gene="rpmB"
                     /locus_tag="ACL73L_000226"
     CDS             complement(13604..13792)
                     /gene="rpmB"
                     /locus_tag="ACL73L_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HGS0771279.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSCKVERV"
     gene            complement(13911..14345)
                     /locus_tag="ACL73L_000227"
                     /pseudo
     CDS             complement(13911..14345)
                     /locus_tag="ACL73L_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163337.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(14821..16365)
                     /locus_tag="ACL73L_000228"
     CDS             complement(14821..16365)
                     /locus_tag="ACL73L_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HGS0771280.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDD
                     IELLNEAGNEFSEEAILAGELTPVFFGSALTNFGVQTFLEIFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVINAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMSPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            16578..16880
                     /gene="gatC"
                     /locus_tag="ACL73L_000229"
     CDS             16578..16880
                     /gene="gatC"
                     /locus_tag="ACL73L_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HGS0771281.1"
                     /translation="MKITQEEVTHVANLSKLRFSEEETAAFATTLSKIVDMVELLGEV
                     DTTGVAPTTTMADRKTVLRPDVAEEGTDRDHLFKNVPEKDNYYIKVPAILDDGGDA"
     gene            16880..18346
                     /gene="gatA"
                     /locus_tag="ACL73L_000230"
     CDS             16880..18346
                     /gene="gatA"
                     /locus_tag="ACL73L_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HGS0771282.1"
                     /translation="MTFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDADNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLNAIASEDAKDSTSAPVRIADFTSKIGQDIKGMKIALPKEYLGEGINPEVKE
                     TILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVG
                     LQLIGPKYSEETIYQAAAAFEATTDYHKQQPVIFGGDN"
     gene            18346..19788
                     /gene="gatB"
                     /locus_tag="ACL73L_000231"
     CDS             18346..19788
                     /gene="gatB"
                     /locus_tag="ACL73L_000231"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HGS0771283.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDIHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEVELEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEVQRQAEILRSGGQIRQETRRYDEANKATILMRVKEGAADYRYFPEPD
                     LPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLTANKVTSDFFEKAVA
                     LGGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMITIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAILIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVALKLLAQELAKLKEN"
     gene            19912..20955
                     /locus_tag="ACL73L_000232"
     CDS             19912..20955
                     /locus_tag="ACL73L_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-butanediol dehydrogenase"
                     /protein_id="HGS0771284.1"
                     /translation="MATMKAARWHAAKDVRIQEIEVPEVLPHQVKVAVKFTGICGTDL
                     HEFLDGPIFIPTDEHVYSGQKAPVTLGHEFSGEIIEVGSDVTRVKVGDRVAVEPILAK
                     NNLVGDYNLDPNLNFVGLAADGGFAKYCVLDGDLVHVIPDSLSYEQAALTEPAAVAVY
                     AVRQSSLKAGDTAVVFGLGPIGLLIVEALRAAGASKIYAVELSPERQAKAEELGAIVV
                     RPEEGETAVEAIHRLTNGGVDVSYEVTGVPVVLGQALAAVHKAGECMVVSIWEREANI
                     NPNEFAIQEKTLKGIIAYRHIFPKVLELMEQGYFSAEKLVTKKIKLENIVEEGFIELT
                     QDKSQIKILVEPE"
     gene            complement(20980..21210)
                     /locus_tag="ACL73L_000233"
     CDS             complement(20980..21210)
                     /locus_tag="ACL73L_000233"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771285.1"
                     /translation="MVNVKEYPLYFFSYSMEIKEQTRKLAASCSKHCFEVVDRTDEVS
                     NHIYGNVTLTWSEEIFEEYYISKGRSKFTPTC"
     gene            21333..21893
                     /gene="efp"
                     /locus_tag="ACL73L_000234"
     CDS             21333..21893
                     /gene="efp"
                     /locus_tag="ACL73L_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HGS0771286.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            21915..22304
                     /locus_tag="ACL73L_000235"
     CDS             21915..22304
                     /locus_tag="ACL73L_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HGS0771287.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGRGIYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            22297..22719
                     /gene="nusB"
                     /locus_tag="ACL73L_000236"
     CDS             22297..22719
                     /gene="nusB"
                     /locus_tag="ACL73L_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HGS0771288.1"
                     /translation="MTSPLLESRRQLRKCAFQALMSLEFGTDVETACRFVYTHDREDT
                     DVQLPAFLIDLVSGVQAKKEELDKQITQHLKAGWTIERLTLVERNLLRLGVFEITSFD
                     TPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ"
     gene            complement(22768..22962)
                     /locus_tag="ACL73L_000237"
     CDS             complement(22768..22962)
                     /locus_tag="ACL73L_000237"
                     /EC_number="3.4.26.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HGS0771289.1"
                     /translation="MILSHRDPISLLYYSLIGFFFALVYRSTDNLRLTILCHSFFNFL
                     NHAKPIWIFVYNYIYYHFFR"
     gene            complement(23017..23492)
                     /locus_tag="ACL73L_000238"
                     /pseudo
     CDS             complement(23017..23492)
                     /locus_tag="ACL73L_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246431.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(23510..23695)
                     /gene="briC"
                     /locus_tag="ACL73L_000239"
     CDS             complement(23510..23695)
                     /gene="briC"
                     /locus_tag="ACL73L_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
                     /protein_id="HGS0771290.1"
                     /translation="MTGTNTFTVLSTEDLEQTSGGLAVWEDGYSRWLYYREFAPYMRQ
                     GALNSYIDAWKYGFRTG"
     gene            complement(23958..24725)
                     /locus_tag="ACL73L_000240"
     CDS             complement(23958..24725)
                     /locus_tag="ACL73L_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HGS0771291.1"
                     /translation="MNIAKIVREAREQSRLTTLDFATGIFDEFIQLHGDRSFRDDGAV
                     VGGIGWLGDQAVTVVGIQKGKSLQDNLKRNFGQPHPEGYRKALRLMKQAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGTRAMEAAELMKITSHELLEMDVVDKVISEVGLSS
                     KELIKSVKKELQTELARLSQKPLEELLEERYQRFRKY"
     gene            complement(24722..25588)
                     /gene="accD"
                     /locus_tag="ACL73L_000241"
     CDS             complement(24722..25588)
                     /gene="accD"
                     /locus_tag="ACL73L_000241"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HGS0771292.1"
                     /translation="MALFSKKDKYIRINPNRSVREKPQAKPEVPDELFSQCPGCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLALTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     SMREKTGLHEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEKL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNAGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            complement(25625..26992)
                     /gene="accC"
                     /locus_tag="ACL73L_000242"
     CDS             complement(25625..26992)
                     /gene="accC"
                     /locus_tag="ACL73L_000242"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HGS0771293.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGHVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVEKPDDLVSAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAEFVGYENAGTIEF
                     LLDEASSNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQICIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNADFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            complement(27004..27426)
                     /gene="fabZ"
                     /locus_tag="ACL73L_000243"
     CDS             complement(27004..27426)
                     /gene="fabZ"
                     /locus_tag="ACL73L_000243"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HGS0771294.1"
                     /translation="MIDIQGIKEALPHRYPMLLVDRVLEVSEDTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVLIMEALAQTAGVLELSKPENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFVKRRGTIAVVEAKAEVDGKLAASGILTFAIGN"
     gene            complement(27423..27908)
                     /gene="accB"
                     /locus_tag="ACL73L_000244"
     CDS             complement(27423..27908)
                     /gene="accB"
                     /locus_tag="ACL73L_000244"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HGS0771295.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPAPVLATPSPVAPTSAPAETVAEEVPAPAEASVATEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            complement(27911..29146)
                     /gene="fabF"
                     /locus_tag="ACL73L_000245"
     CDS             complement(27911..29146)
                     /gene="fabF"
                     /locus_tag="ACL73L_000245"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HGS0771296.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFVKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAIKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEANVVYGQGLEKEIPYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            complement(29168..29899)
                     /gene="fabG"
                     /locus_tag="ACL73L_000246"
     CDS             complement(29168..29899)
                     /gene="fabG"
                     /locus_tag="ACL73L_000246"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HGS0771297.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQVGANIVLNSRGAISEEL
                     LAEFSNYGIKVVPISGDVSDFADAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGAFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVIAIDGGLSM"
     gene            complement(29933..30853)
                     /gene="fabD"
                     /locus_tag="ACL73L_000247"
     CDS             complement(29933..30853)
                     /gene="fabD"
                     /locus_tag="ACL73L_000247"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HGS0771298.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPIVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVASGA
                     LDFEDAVALVAKRGAYMEEAAPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTALLEPASQKLAETLAQVS
                     FSDFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDQTAHLAHVEDQASLVALLEK"
     gene            complement(30846..31820)
                     /gene="fabK"
                     /locus_tag="ACL73L_000248"
     CDS             complement(30846..31820)
                     /gene="fabK"
                     /locus_tag="ACL73L_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HGS0771299.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     ISIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVD
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWAGVVRND"
     gene            complement(31939..32163)
                     /locus_tag="ACL73L_000249"
     CDS             complement(31939..32163)
                     /locus_tag="ACL73L_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HGS0771300.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQAK"
     gene            complement(32223..33197)
                     /locus_tag="ACL73L_000250"
     CDS             complement(32223..33197)
                     /locus_tag="ACL73L_000250"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HGS0771301.1"
                     /translation="MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGITGKELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANDRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLIPLDGSQTVLLSGFGGGLTWGTLILTI"
     gene            complement(33197..33631)
                     /locus_tag="ACL73L_000251"
     CDS             complement(33197..33631)
                     /locus_tag="ACL73L_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HGS0771302.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGRLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
     gene            complement(33725..34510)
                     /locus_tag="ACL73L_000252"
     CDS             complement(33725..34510)
                     /locus_tag="ACL73L_000252"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HGS0771303.1"
                     /translation="MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRVSSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            34879..36243
                     /locus_tag="ACL73L_000253"
     CDS             34879..36243
                     /locus_tag="ACL73L_000253"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HGS0771304.1"
                     /translation="MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYRDYVAGNDISKSQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPIEENEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREIGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRQLTPIKEEEILRQLVQKAKVDHAEIEHDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGE
                     SKED"
     gene            36246..36617
                     /locus_tag="ACL73L_000254"
     CDS             36246..36617
                     /locus_tag="ACL73L_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HGS0771305.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNVE
                     QFIQIPWSHINQIGANVTGRKISRHFEVFTDKGKFLFASKDSGAILKIAREKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            36830..38104
                     /gene="serS"
                     /locus_tag="ACL73L_000255"
     CDS             36830..38104
                     /gene="serS"
                     /locus_tag="ACL73L_000255"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HGS0771306.1"
                     /translation="MLDIKRIRTDFEAVAEKLATRGVDAAVLNEMKEIDAKRRNILVK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEVKALDAELAEIDAKLTEFTT
                     TLPNIPADSVPVGADEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLTNYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSATKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEALRPYMGGAEVIKP"
     gene            complement(38129..39028)
                     /locus_tag="ACL73L_000256"
     CDS             complement(38129..39028)
                     /locus_tag="ACL73L_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HGS0771307.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDTLPVLSHLFSLTGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIAGFTIKFACPGRRVHATPSWTVLYVGIAVAALTYPLVGIIEIAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTSLPTWVLIVMIL
                     ASQSFFFFVLTRLPNILKQGFQPAFSALTFPTIITATSLKMAQGILKLPFLDYLVLAE
                     TIICLTILFCVLGAYLIWLRKKV"
     gene            complement(39138..39686)
                     /locus_tag="ACL73L_000257"
     CDS             complement(39138..39686)
                     /locus_tag="ACL73L_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HGS0771308.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNRTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLA
                     ILCNYANNLANTPINPELQPYA"
     gene            complement(39904..41226)
                     /locus_tag="ACL73L_000258"
     CDS             complement(39904..41226)
                     /locus_tag="ACL73L_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HGS0771309.1"
                     /translation="MLELLKSIDAFAWGPPLLILLVGTGIYLTMRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFAALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKYAEGLLAIKYRTKDDHGAVAGGPMHYILLGMGEKWRPLAVLFAVAGVLVALLGIG
                     TFTQVNSIAESIQNTTTISPAITALVLSVFVAIAVFGGLKSISKVSTTVVPFMAIIYI
                     LGTLTVIFFNIGKIPGTIALVFTSAFSPLAAVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILVTGVWSGDLNGVALT
                     QSAFSTVFSHFGPALLTIFLVLFAFTTILGWNYYGERCFEFLFGVRFIWLYRVVFVLM
                     VLLGGFIELDMVWIIADIVNALMVLPNLIALLVLSPVVIAETKKYFDK"
     regulatory      complement(41317..41405)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(41569..43905)
                     /locus_tag="ACL73L_000259"
     CDS             complement(41569..43905)
                     /locus_tag="ACL73L_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HGS0771310.1"
                     /translation="MNKKILETLEFDKVKALFEPHLLTEQGLEQLRQLAPTAKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGADLNIEEFLLLKRVLLASREL
                     QNFYTNLENVSLEELALWFEKLHDFPQLQGNLQAFNDAGFIENFASEELARIRRKIHD
                     SESQVRDVLQDLLKQKAQMLTEGIVASRNGRQVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHAAEIANDAWIIGHLDLIRA
                     KVRFIQERQAVVPQLSENQEIQLLHVCHPLVKNAVANDVYFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVMAQSGLPILADKGSRVGIFEEIFADIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQIDQD
                     NDVNRIIEQLEEQTLESRKRLDNIREVEQENLKMNRALKKLYNELNREKETELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKAKLKKLAPEKVDLSKNKVLQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDGRWEAQVGLIKMTLEEKEFDLVQAQQEKPV
                     KKKQVNVVKRTSGRGPQARLDLRGKRYEEAMNELDTFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFKG"
     gene            complement(44009..44557)
                     /locus_tag="ACL73L_000260"
     CDS             complement(44009..44557)
                     /locus_tag="ACL73L_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HGS0771311.1"
                     /translation="MISFLLLLVLVWGFYIGYRRGLLLQVYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEGQGTFFFPSDQLFQLDKVFYAGIGYLLVFGIVYSIGRLLGL
                     LLHLIPSKKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPITTSWLKQIWVTNLIG"
     gene            complement(44554..44856)
                     /gene="zapA"
                     /locus_tag="ACL73L_000261"
     CDS             complement(44554..44856)
                     /gene="zapA"
                     /locus_tag="ACL73L_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HGS0771312.1"
                     /translation="MANLNRFKFTFGKKSLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSADDETIALLLAVNCLSTQLSREIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            44943..45824
                     /gene="rnhC"
                     /locus_tag="ACL73L_000262"
     CDS             44943..45824
                     /gene="rnhC"
                     /locus_tag="ACL73L_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HGS0771313.1"
                     /translation="MASITLTPSEKDIQAFLEHYQTSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFGYQLVEENRGQNLPLIGTDEVGNGSYFGGLAVVASFV
                     TPDQHAFLRKLGVGDSKTLTDQKIRQIAPILKEKIQHQALLLSPSKYNEVIGDRYNAV
                     SVKVALHNQAIYLLLQKGVQPEKIVIDAFTSAKNYDKYLAQEANCFSNPISLEEKAEG
                     KYLAVAVSSVIARDLFLENLENLGRELGYQLPSGAGTASDKVASQILQAYGMQGLSFC
                     AKLHFKNTEKAKKRLER"
     gene            45828..46442
                     /gene="lepB"
                     /locus_tag="ACL73L_000263"
     CDS             45828..46442
                     /gene="lepB"
                     /locus_tag="ACL73L_000263"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HGS0771314.1"
                     /translation="MNSFKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKHLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQAFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRFWPITRIGTF"
     gene            46721..49087
                     /locus_tag="ACL73L_000264"
     CDS             46721..49087
                     /locus_tag="ACL73L_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HGS0771315.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTMADVIEGEDYTFWGQIVQHSKYGEQLQISRYDRAKPTSKGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGIAGLSAKNREAFVSTLRLNYGTEMVLAKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQACMETGDTYVEARDLLEQTLTLLESSRPVELDPSQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLVRILEKGKQKSHDLETIQKHITTIEEELGIEYDN
                     IQKQAICDAIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDLRKKSNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDADFIIVDEFSMVDTWLANQL
                     FSNISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTRLEKIYRQSEESTIVTLA
                     SQIRQGILPADFTQKKADRSYFEIASGHIPATIEKILGAALRNGIPARDIQVLAPMYR
                     GTAGIDAINQLMQDLLNPPQKDQLSFEAPQCHYRKGDKVIHLVNDAEINVFNGDLGAI
                     TDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAMSIHKSQGSEFPVVILPI
                     TSASRRMLERNLIYTAITRAKSKLILLGELQAFDYATQHIGTARKTYLIERFSDLLEN
                     VEEKQQAVSETVTSSASEQSYILTEENWDSIPAMIGITDTDLKEIFGK"
     gene            complement(49135..50418)
                     /gene="tig"
                     /locus_tag="ACL73L_000265"
     CDS             complement(49135..50418)
                     /gene="tig"
                     /locus_tag="ACL73L_000265"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HGS0771316.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDQKFGEEALYQDAMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVIAAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
                     ENGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVT
                     FPEDYQAEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYRKEL
                     AAAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQSLLSADMLKHDITIKKAVELITSTATVK"
BASE COUNT        14861 a        11527 c         9588 g        14650 t
ORIGIN      
        1 actattgact tttcttatag tttgtctttc ttctatcttc cattatatca tactttttca
       61 gtctatggta gctctcttta aaaggctccg atactgccgc cacctccgcc tccagagaag
      121 ccaccgccag aacttccact tccagaagat acggagtagg tacttgctgt atttgcgaca
      181 ctagcataat ggctcatttg cgcgcttgaa tgataaaaca tactgtgcca gccataagct
      241 acatagagat tgatatctgg attttcaact tgaatctgat gaaccttcat caaatgacta
      301 accttgtccg catagccaaa taaggttgca tagaccaaga gacgattcca aacaacaata
      361 ctttccaatt cagccttatc cagtcgtgca atctcacgta acatattttc aaaactggtc
      421 cagagatagt agacctcagc tcccgcttca tttagaacac catcacgatt atctagtcga
      481 agcttccaat aatagaaaac agacaaaact aaccctagaa aaccaagtat tggcaaaggg
      541 aggtaaagat agccatgaac gtctaaactg tacaagaaca aaccaaatcc gataaatagg
      601 ggcaagatag tcaagacacc catacccact tgcaaagtct tttccaaacc agttaaagga
      661 cgatagtaat ctgggagccc ccagaaggaa actcgttttc tcactccttc ttgcatctgg
      721 ttcaatacct cttcaaaaga agatttgagt tgaagccctc ttgcttgaat ccgtttttca
      781 tcagaaactt tggctctacg ataaagacta tcagatacct tgtaatccgc aaacaaattg
      841 gaaagagtgg cttctttttt gcctgaaaag gctagattta gacagtctct ctcaaatctt
      901 gacaaaccat cttctttcac caatctcaag ccaactgcat ctccttctga aataatagag
      961 acattcccac ggtctatcac atctagcaag gtagcttgaa taagttgatc aaaggtgaat
     1021 tttccagctc ccttgaccaa gggactcact tcctccaagg aggtcgagta gactgcttct
     1081 gataaaacca taggctctaa ttccattggt ggttcataga gacgatgatt tttggcatat
     1141 ttgactgaag gagtggtctt tcttctataa ataaaataga agcagacact caataacaag
     1201 gagatagaaa ggatcgaagg gaggacccaa gtaacgagtt gtttactctg atctttttct
     1261 ctaacaatcg agtcttctat cttattaaac tcttctaaac gattcccttt caagccctga
     1321 tccctagcgc tagcaaaatc ggtccgagac caataggcat gcaactcaac tccacgctta
     1381 gccggaagat tgtctaaacg gatagtataa tcaaggttac tcttttcaat cgttccctct
     1441 ctaaaaagtt tccctgtatg gaaaaagagt ttttcagccc ccttgtctcc ccttacatga
     1501 aattcaaact tttcaataga ctctgaacta tctgtcagag gttgccaatt taattcagcg
     1561 atatcatcat aaaggaaaag taaatttttt aagttccaga cgaggtcaac ttcaactatg
     1621 tcgccctcct gacctggatt atagactctc acagtataac catccgcttc ttctgttact
     1681 tcgctagtca catctgctag ttctgcaccg tttttcgcag cctgaacctt tggatgaggg
     1741 tcaatgtcaa acccgctagg catcttacca gcacgtccaa gtcccacgat ttggccctta
     1801 aagtcctcct caaactggta aactatcttc tgtctaaact ctgccgtatt atctgcatga
     1861 atatacaaat ccccttgata agagtttatc ttgaaatcaa tggcaaaaac agagagtgga
     1921 agaaggcaaa acaagcctaa caccagtaag aaaaaagttt ttttcatcaa ctaagccccc
     1981 tttttatctt tcttatcatt atagcatttt ttcgtaagta aaggcttact tatgttgaaa
     2041 aattacgcat ttttcgataa aataggagac agttattttt taatagatca gaataggaga
     2101 aatcatgaga aaaatatact tatctatttt cacaagtctc ttgctgatgc taggacttgt
     2161 caatgttgct caagccgatg aatatttacg catcgggatg gaagcagcat atgctccctt
     2221 taactggacc caggatgatg atagcaacgg agctgtcaaa atcgatggga ccaatcagta
     2281 tgccaacgga tacgatgttc aaatcgccaa gaaaatcgct aaggacttag gtaaagaacc
     2341 tttggttgtt aaaaccaagt gggaaggtct agtccctgcc cttacttctg gtaagattga
     2401 catgattatc gcaggtatga gtccaactgc agaacgcaaa caagaaattg ccttttcgag
     2461 cagttactac actagcgaac cagttctact ggtcaaaaaa gattctgcct atgcaagtgc
     2521 taaatctttg gatgacttta atggtgcgaa aatcacttct caacaagggg tctaccttta
     2581 tgacttgatt gcacaaatca caggtgctaa aaaagaaaca gccatgggag acttcgctca
     2641 aatgcgccaa gctcttgagg ctggtgtcat cgacgcctat gtttctgaac gcccagaagc
     2701 tctgactgcc gaagccgcta actctaagtt caagatggtc caagtagaac caggtttcaa
     2761 aactggggaa gaagatacag ctatcgccat tggacttcgt aaagatgaca ctcgtattag
     2821 ccaaatcaat gccagcattg aaaccatttc aaaggacgac caagttgccc tgatggatcg
     2881 tatgatcaag gaacaacctg ccgaagctac aacaactgaa gagactagca gtagtttctt
     2941 tagccaagtc gctaaaattc tttctgaaaa ctggcaacaa ctcttgcgtg gtgctggtat
     3001 cactctttta atctctatcg tcggaaccat cataggtctc attattggac ttgccattgg
     3061 tgttttccgt actgctcctc tctctgaaaa caaagccatt tatggccttc aaaaactagt
     3121 cggctgggtt ctcaatgtct acattgaaat tttccgtggt accccaatga tcgttcaatc
     3181 gatggttatc tactatggaa ctgcccaagc tttcgggatc aaccttgacc gtacactggc
     3241 tgctatcttc atcgtttcaa tcaataccgg tgcctacatg actgaaatcg tccgtggtgg
     3301 tatcctagca gttgacaagg gacaatttga agctgcgact gctcttggta tgacccataa
     3361 ccagaccatg cgtaagattg tcctacctca ggtagtccgt aacatcctac ctgcaactgg
     3421 taatgaattt gtcatcaata tcaaagatac atctgtattg aacgttatct ctgttgtcga
     3481 actttatttc tcaggaaata ccgtggcaac acaaacctat caatacttcc agacatttac
     3541 aatcatcgcc gtgatttact ttgtcctcac cttcaccgta acacgtatcc tacgatttat
     3601 cgagcgccga atggacatgg atacctatac tacaggtgct aaccaaatgc aaacggagga
     3661 tttgaaataa tgacacaagc aatccttgaa attaaacacc tcaaaaaatc ctatggacaa
     3721 aacgaagtgc taaaagacat ttcactcact gtccacaagg gagaggtcat ctctatcatc
     3781 ggaagctctg gaagcggaaa atcgaccttc ctacgctcca ttaacctact tgaaacacca
     3841 actgatggac aaatccttta tcatggacaa aacgtcctcg aaaaaggcta tgacctcacg
     3901 caataccgtg aaaagttggg gatggttttc caatccttta acctctttga aaatctcaat
     3961 gttcttgaaa acacaatcgt cgctcagaca actgtcctaa aacgcgaacg cacagaagct
     4021 gaagaaattg ccaaagaaaa cctggaaaag gtcggcatgg gagaacgcta ctggcaagcg
     4081 aaacccaaac aactctcagg tggtcaaaaa caacgtgtgg ccattgctcg tgccctttca
     4141 atgaatcctg acgccattct ctttgatgaa ccaacatcag ctctcgatcc agaaatggtt
     4201 ggagaggtcc tcaaaatcat gcaagatctg gctcaggaag gcttgaccat gattgtcgta
     4261 acccatgaaa tggaatttgc ccgtgatgtc tctcaccgtg ttatctttat ggataagggt
     4321 gtgattgctg aagaaggtaa accagaagac ctcttcacta atcctaaaga agaccgtaca
     4381 aaagaatttc ttcaacgtta tctcatactc aatgaaaatc aaagagcaaa ctaggcagct
     4441 agccgcaggt tgctcaaaac actgttttga ggttgcagat agagctgacg tggtttgaag
     4501 agattttcga agagtatcaa ataaaaagaa aaggctgcat caaccgtgca gtctttttgc
     4561 tgtcctcaaa atgaaaaggc agattctcta tctctactac ttgaaggtca agaatccgcc
     4621 gttatccaaa gattaatctt caaatttttc ataatttttt tcatagaaat caattaggcc
     4681 tagggctgtt tcaaatgaaa tattttttac ttttgcacga ccctgcgcca gagcaatgat
     4741 agacatttca cgcgcattcg tttctttaga gatacggtag cctgtgattt tcttatcacg
     4801 aacccagcca acaactgatt ctactttttc aaagtttgtc ttagccatgt ttttctccta
     4861 ttttcttctt tataatattt aattgtatac gtttttacta cttttgtcaa taaaaaaaac
     4921 atatattcaa cgaaaataat tagttcagaa ttttcttact tcctttttaa atccgataat
     4981 atataggttt tgctagcctc tacaccataa atatttgcta ataaaaatac tgtattatta
     5041 ccctcttaag gtgcgaaatt attcaagttc ttaaaaatgc ttctctattt ccgtaagata
     5101 gcttatttct tcgatttaag aactgaagat gatagttcta acctttagaa atccaagcct
     5161 tcactagctc aaaaactcct atctttcttc ctcaataaat catctgagag ataggtattc
     5221 cttatatcct tcaaattcat cactcctttg gatattgagg tgctagccta gaagcagaca
     5281 cccgattatc taaaccatcc ttaataaaga tgacaagaca gataaactca gttaattact
     5341 tatatatcct tttaatatgg tatatttgtc aacaaaaaga aatcaaatca taccttatac
     5401 tcaatgaaaa tcaaagagca aactaggaag ctagccgcaa gttgctcaaa acactgtttt
     5461 gaggttgcag atagagctaa cgtggtttga agagattttc gaagagtatt aaagacagtg
     5521 tgactatgct tgtcaataga taggaaatca aaatgaagga tggaaatctg agccaaacat
     5581 cataagtgct ataatgaaca gaagtaagaa ttgcacgata agttttctaa aaccatagga
     5641 atcgtcccct ttcttcatct tcattatagc acatatacat cagttgtaca aataatatga
     5701 tattttttta ttagtcctca gacaagcata ttataaagcg tttcattacc atttaagtta
     5761 atataagctg gatcaaaacc ttccattcta cggatcaaac ctgcataatc atgcttatct
     5821 gccaaagcga tcccaatcaa tactgggcct gttcccttgc tagctcgttt gatatactca
     5881 aaacgtgtga tatcatcatt tggccccagg atatcattta caaactcacg caaagctcct
     5941 ggacgctgtg gaaaattgac cacaaagtaa tgtttgatac catcataaat caaggcacgc
     6001 tcttccattt ctggcatacg gttgatatca ttatttcctc cagaaatgat acaacaaatg
     6061 gttttcccct taatatattc agctaaaacc tctaaagagg cgatactagc cgctccagca
     6121 ggctctgcga ctatcccttg cttagagtaa aggtcaatca aggtttcaga aatcaatccc
     6181 tcatcgacac ctaccaaagt ttgaatatgt tgacgagttg cttcataggt caactgacct
     6241 accttttgca cagcaatccc atccgcaaat ttatcaattt ccttgagttt gactggacct
     6301 ccagcctcaa aggcagcttt catggaacgc gctccattcg cctctactcc gataacctca
     6361 atctctggac ttgtttcctt gatataggta gaaaccccgg caatgagacc gccaccacca
     6421 acaggaacca agacagcatc aaaatcaatc gattcttttc gagcttcttc taaaatctca
     6481 taagcaactg ttccttgacc tgcttgaaca tgagcatcat caaaaggatc aataaaggta
     6541 cgattttcag agactgtaaa ttcttgagct gctttggctg aggcatcaaa ggtatctcca
     6601 actagtttaa tagttacaaa atccccacca aaaaagcgaa cctgaccaat cttttgttgt
     6661 ggcgtagtaa taggcataaa gatagtagca ggaattttca tttcattaca agtataggct
     6721 actccctgcg catgatttcc cgcagaagcg cagactaccc cacgttcacg ttcttccttg
     6781 ctgagctggg aaatggcata ataggcacca cgaattttaa aggagcgaac acgctgggca
     6841 ttttcttttt tcaaataaat cttagcacca tacttctccg ataaataatg atcgtaatcc
     6901 agtggagtat tcacaaccac accgttcaag accttgtgag ccttgattat atcttttgaa
     6961 cttaacatat aaaacctctt tttctataaa aaacagtcac taatgactgt tttttagtta
     7021 ttcatctccg aaaacttcag taacactttc atggtattta tgagccatac gaatcgaaaa
     7081 ctctttagga agttcataac gatttgtgga aatccaatca tataaccatt ctaccaaact
     7141 ttcttcacta tcattacgat ggaagataaa atagtttaaa atttctttat attctacatc
     7201 attcaaatcc aacgtgtcta caaattgact acgaggaaca aaattagaat ctaattttct
     7261 tagttgatct gtcaattctc ccaattgtaa atccaactga aaaaatagtt tttgcagttg
     7321 cctatcagtt tcatcacatc ctgtcttgat agaaaacaaa ttggtaatat tcatatgatt
     7381 tcaaccccta aataagtaca tctaatatcc cacgaatagc tgaagctaaa gttttcgcag
     7441 tccagtcatt taaacccgat tctttcaatc cagatgcaat aatttgctct atttgatata
     7501 aaccgccatc tatcgtctca tttattctgc gaagaggacc ttcaatatag gataaagctt
     7561 ctccgaatct aacagctgat ttaggtccta atgatttacc aactatattt gttaatattt
     7621 tttatttctc aaaatccatt tcgctatata acgagcagat ttagtcgcac cagctctctc
     7681 aaacggagaa tgaatttcaa gaccttcttc tctagctatt tgtgccaatt tgaagaattg
     7741 ttcaaaggtc atatgcaacc taccatcttc aaaattagca tcatcataaa tgttaattac
     7801 agcattttca tcatacgacc aatctgcttt attttggtct gctagcacta ttaccgacgg
     7861 tgtaattaca ttatacaact agaaccaata ataacgatgc aatttttctc ataatctttt
     7921 tcataaaatc atctccagcg ctatatattt ctaattagtt atagattttg aatgcatcat
     7981 cgtcgttttt accaacgaat ggcattgctt tacgcaattc tgcaccaact ttttcaattt
     8041 caaggttagc tgcttgttca cggtaagcag tcaattttgg acgtccagct ttatagtcat
     8101 ttacaaagtc atttgcaaat ttaccatttt ggatgtctgc caagacagcc ttcatatttt
     8161 ctttaacttg ttcagtgatt acacgtggac ctgatacata gtcaccgtat tcagcagtgt
     8221 ttgaaataga ttgacgcatt ttcttgaatc caccttcgta gatcaagtca acgatcaatt
     8281 tcatttcgtg aagaacttca aagtaagcca attctggagc gtaacctgct tctgtcaaga
     8341 cttcgaaacc tgcttcgata agggcagtca aaccaccaca aagtacagct tgttcaccaa
     8401 acaaatcttc ttcagtttct tctttgtaag ttgtttcaag aagacctaca cgagccgctc
     8461 caacaccttt acaccagtcc atagcaatgt ttttagcatt tcctgttgca tcttggtata
     8521 ctgcataaag agctggaaca ccaaatcctt cttcgtaagt acgacgtacc aagtgtcctg
     8581 gtcctttagg agcacacatg aagacatcta catccgcagg aactttgata aattcaaagt
     8641 ggatgttgaa accatgggca aatccaactg cgtttccagc ttccaagttt ggagcgattt
     8701 ctgcttcgta caattcttgt tgaatttcgt ctggcgccaa gatcataata acatcagcca
     8761 acttagtagc ttctgctact gtgtaagtat caaatccatc ttcttttgct ttatcaaaag
     8821 atttacctgg acgtacaccg ataataacgt cacgacctga atcacgcaag ttttgagcat
     8881 gcgcatgccc ttgtgaacca taaccgataa cggcgatttt tttaccgtca agtgctgcta
     8941 ctttaacatc tttttcatat tccatttgaa ctgtcatagc ttttctctct tttctattat
     9001 ttattgcctt ttaggctggt ttaataaatt taagttggat ttttaatcgc gggtaaatcc
     9061 agttgcaccc gttcgagcaa tattgcgaat accgtatggg cgaatgactc gcaatagggc
     9121 ttcgctcttt tctgcatttc ccgtcatctg aatggtaatc gagcttggcg ctacgtctac
     9181 tactgttgca cggaaaggtt gaataatcgc taaaatctca gctctcttct cagctggcgc
     9241 tgacatctta accaaaatca cctcgcgctc caaatgaggc ttgtctgtaa tatctcgaat
     9301 gcgaatcaca tcaatctgac gattgagctg tttgatgatt tgctccactt catcatgaga
     9361 agcaacatca ataataatag tgatacgcga tacattcgga tcttctgttg ctccaacaga
     9421 gatgctttca atattaacct gacgacgaga taggacacct gtaaagcgat tgaggactcc
     9481 tgaacgattt tgtagttttg ctgttaacat tctacgcatg gaactgcacc cccaacatct
     9541 catgattact cttaccagcc ggtaccattg gtaacacctg ttccttacga gaaatatcta
     9601 cctcaattag cataggaaca tcctcagtga tgacttcaag gtcttgagcc aaggtctcag
     9661 gattgtcaaa cttatagttt ttaataccat aagcctgcgc catcaattgg aaatcaggaa
     9721 gggtatcaaa gaccgactct gatgttctgc cttcatagaa ggattcctgc cactggcgaa
     9781 ccattccaag tgaatgattg ttcagcataa ccaccttgat tggcaccttg taaatattca
     9841 aaatagccaa ctcctggttg gtcatttgga aaccaccatc cccaacaaac aagactactt
     9901 ccttatctgg gttagcaatt ttagcaccga ttgctgctgg aattccaaag cccattgttc
     9961 ccaaaccacc tgaagtcact aactgacgtt cattttggta gggataatac tgagctgtcc
    10021 acatttggtg ttgaccaacg tctgttacca caatggcatc tccattcgtc aattcaccaa
    10081 ttcgttcaat aactgcttgc ggttgaacca cacgctcttt cttgtcatac gaacgaacac
    10141 gattcttgtc tttagtgact ttctcaatcc acttttcagt attgttgtga actgttggtt
    10201 ctgctagcaa catttgcaag gccttcttag catctccaac tacaggaatg tctgcactga
    10261 taatcttgcc aatctcagct gggtcaatat caatgtgggc aaccttagca ttcttagcga
    10321 aagtcttagg attccccgtc aaacggtcat cgaaacgaga accaatacta atcataaagt
    10381 ccgcttccgt catggcaata tttgctgcga atgacccgtg catgcctccc attccaagaa
    10441 agagtgggtg actcgttgca atcgttcctt gtcccaaaag actggttacc actggaattt
    10501 gatagcgttc tgcaaattca tttagttccg tagcagcctc agcataacta attccaccac
    10561 cagctaacaa gactggcttt ttagccttgg acaattgctt caagattttc ttgatttgca
    10621 tatcattcgg ctcaagagtc ggctgataac ttggtaaatt cacttctggt gaataaatga
    10681 agtctgtttc taaagcagat atgtcttttg gtaggtcaat tacaactggc cctggacggc
    10741 ctgtagttgc gatatggaca gcttccgtaa tgatacgcgg aatatcagct gtctcacgaa
    10801 cttggtaatt gtacttagtg attggcatgg taattcctac gatgtctgcc tcctgaaagg
    10861 catccttccc aatccctgct cgcgccacct gacctgtaaa gaccaaaagg ggaacgctat
    10921 cgctcatagc atccgcaatc cctgtaatgg catttgttgc tcctggtcca ctagtgacga
    10981 cggcaacacc caactttcca gttgatttgg catagccttc agcttcatgc aaacaacctt
    11041 gctcatggcg ccctagaatg tggcgaatgc ctttaaaatt atatatcgca tcataaaaag
    11101 gcaaaaccgc accaccagga taaccaaaga tggtatcaac tcctaaatca cgaagtgttt
    11161 ccaaaactag gtccgacccc gtcttaggag attctaaact gattttctcc attattcccc
    11221 tttctttcct cttaaaaata acttgttact atcataccat tttttcaaaa tttttcaaga
    11281 taaaagaaga aattttctga attttctatt ttaacgttta tttatgaatg ttatttttgt
    11341 ttttattaaa aagataccga tttcatttac taaaaagaaa aaagaactga tttctcagtc
    11401 cttcattaat cttattccac actaaatagg tatgggtaaa caggttgttg accttggtga
    11461 atctcgactt caacgtcttc gaattcttct acgatttctt gagcgatttc attggcaagt
    11521 tcttcgcttc cgtcttcacc tacatagaag gttacgattt cactgtcttc atccaacata
    11581 tgtttcaagg tttcagtcaa tgtttggtgc atatcagggt ttgacacaag aatttttcca
    11641 tccaccatac ctaaattatc gttttcatgg atttctaagc catcgatcgt tgtatcacgc
    11701 acggctgttg tgacgcttcc gctaacgaca tcgctaagag cagctgtcat acgctcttgg
    11761 ttttcttcaa tggacttgct tggatcaaag gcaagaagac ttgtcatacc ttgaggaaga
    11821 gtgcgagcct ctaccactac cgctggttgc tccaaaactt ctgccgcaga ttgagctgcc
    11881 atgaagatgt tcttgttgtt tggcaagaag atgatgttac gggcattaac ctgttcaaca
    11941 gccttgataa agtcttctgt tgaagggttc atggtttgac cgccttcgat aacataatcc
    12001 acgccttgag aacagaagat atctgctaga cctttaccag ccaccacagc aatcaaagca
    12061 tactcttttt cttcagccga cttgataact tgagtagctt ctttctcaac ctgtgcttcg
    12121 tgttggttac gcatattgtc aacttttacc ttgaccaagc taccatattt gagaccttct
    12181 tgcataacaa gtcctggatc ttctgtatga acatggactt tgacaatttc atcatcgtta
    12241 acaacgagga gagaatctcc aagctcatcc aagtagttac ggaattcatc gtagtcaaaa
    12301 tctttagcat aggttggacc ttgcttaaga gctaccatga tttcagtaca gtaaccaaac
    12361 gtgatgtcct cagtcgctac gtgaccagct acagacttat gatgctctac attgatcatc
    12421 tcactcatgt tggcaggagt cgctacaaag tcctcagatg caatatattc gccagtaagg
    12481 gctgaaagga aaccttcgta gatgaagacc aatccttgac cacctgagtc cacaacgcca
    12541 acttctttca atactggaag catgtctggt gttttagcta gagctgtttt agcaccttcc
    12601 aaggctgcgc gcatgacttc aacagcgtca tctgtttgct cagctttttt cttagcaccg
    12661 atagcagctc cacgagaaac tgttaaaatc gttccttcaa caggtttcat cactgcctta
    12721 taggcaactt ccacacctga ttggaaggcc agagccaagt cttgacctgt taactcgtct
    12781 ttatccttga tagcttggga aaatccacgg aaaagctgag acgtaatcac tcctgagttc
    12841 ccacgcgcac ccatcaaaag ccctttggca agaatgctcg ctacttctcc aactgtagaa
    12901 gctggcttgt ctgcaacttc tttagcacca ttttcaatgg tcattcccat atttgtccca
    12961 gtatctccat ctggaactgg aaagacgttt aatgaattga catattcagc ttgcttattc
    13021 aagcgagttg atgcagcctg caccatttct tgaaataagc tagtagtaat ttttgacacg
    13081 attattctcc tacaactttg atattttgaa tgtagacatt tacagtctga gcagtaattc
    13141 caagctggtt ttccaagcta aaggcaacac gctcttgaat gttttttgac acttcactaa
    13201 tctttgttcc gtagcttaac acggtatata catcaactgc aatactgcca tcttcggctg
    13261 cctttacgac gacaccttta gaataatttt ccttacctag cagggcttgg aaattatctt
    13321 tgagggcatt tttactagcc ataccgacca caccaaaaat ctcagttgct gctccaccta
    13381 cgacggttgc aatcacttca tctgttagtt cgatttgacc atcttttgta ttaattttta
    13441 cagtcatcct ttttacctca actagttgat actctatttt atcatatttc agcccaagtg
    13501 taaaagcaga atgatgtatc agcggatatt tactctattt ttcaaacgat gttataccca
    13561 caataaaaga aaaaagaccc taaggtctcc tttgctttta ttattaaacg cgttcaactt
    13621 tacatgattt caaagcacga gctgaagccc aaactttttt aggtttacca tcgataagaa
    13681 cagtaacttt ttgaaggttt ggttttacgg cacgttttgt ttggttcatc gcgtgtgaac
    13741 ggttgtttcc tgatacagtc ttacgacctg taaagtaaca tactttagcc attgtgtttt
    13801 cctcctatta gatctaatat agcggatgtg ctagcaccac ataccgtact atgttatcac
    13861 actttcttgt tttttgcaag ggaattggaa gattttttat ttgtgtctta aatcaggtct
    13921 tgcgtgacat tttctgctct ccacatgcca ttgttgatta acagaacacc agaattaaaa
    13981 ttatgtgtat aaaaatcatc tctaactgca gctaagggta tagccgtcaa gtccaaatcc
    14041 cacagctcat ctatcgattt tcttacaaca atatctaaat ccaaatacag tacacgagac
    14101 tcgcttacat actttggaat aaaatatcta aaaaagccgc atatgaaagt ccctcaaagg
    14161 ggagacgata acctttcaga atactactgt caatctaaac attcacaatc tcactattca
    14221 aagtctctag tcttttttcc atcaattgga accattctcg cggaaggtca tcattaaaaa
    14281 cataaaactt aagattataa tagatgaaca caaagagatt ttattgttgt ttcaacttta
    14341 tccatataag cattatctgc acctaagaca atcgcttttt tctcttcttt cactttttat
    14401 ctcatttctt tttattccca tcatatgttt ctacgtaacc attattttcg cctattcgtt
    14461 cgtaaaacca taccagtgga gattttagat gaagtcccat tacggtttac aatttttaca
    14521 ttacgacacg gagttttaca aatcgatttc atttgccaaa cgtagttagt aaggcagtta
    14581 gctaggctac ctactttctg ctacgacgga aaaatccaca aatcaatacc attcgtggat
    14641 ttttcctagt gaagcgttta ggtaagccgc catagcggtt accgattatt agattacgac
    14701 acggagtttg gcaaatcgat tctatttgcc aaacgtagtt agtgaggcag ttagctagtt
    14761 cgccaaatag cgactagcgt ccaacaattt ggaactttag ttccaattgt tggtactgag
    14821 tcacatcttc tcctctaact ctacgtctgg atacttgtcc gcaaaccagc ggagggcaaa
    14881 gtcattttca aagagaaaga ctggttggtc aaaacggtct ttggctaaga tattgcgact
    14941 tgacgacatc cgttcatcca agtcctcagg cttgatccaa cgaacggtct ttttacccat
    15001 tgggctcatg accacttccg cattgtattc gccttccata cggtgtttaa agacttcaaa
    15061 ctggagttga ccaacagctc ccagcatgta ctcacctgtt tggtaattct tataaagctg
    15121 aacggctcct tcttgcacca attgctcaat ccccttgtgg aaggattttt gcttcataac
    15181 attcttagca gaaactttca tgaaaatttc aggagtaaag gttggcagtg gttcaaattc
    15241 aaacttgttt tttccaaccg tcaaggtatc cccaacctga taagtaccgg tatcgtaaac
    15301 cccgataata tcacctgcca cggcattgat cacattctca cgactttccg ccataaactg
    15361 ggtaacatta gatagtttag cccccttacc agtacgaggg agattgacac tcatgccgcg
    15421 ctcaaattcg cctgatacga tacggacaaa ggcaatacgg tcacggtgac gaggatccat
    15481 gttggcttgg attttaaaga caaagcctga gaaatccttg tcataaggat ccacaatttc
    15541 accgtctgtt ttcttgtgac catgtggttc tggagcaaac ttgaggaaga tttcaaggaa
    15601 ggtctgcaca ccaaagtttg tcagggctga accgaaaaag acaggcgtca attctccagc
    15661 cagaatagct tcctctgaaa actcattccc agcttcattt aaaagctcaa tgtcatcctt
    15721 gacttgctcg tagaaaggat tgctaccaaa gagtttgtcc ccgtcttcta gactggcaaa
    15781 acgctcatcc cctttgtaaa gctctaaacg ttggttatag aggtcataca agccctcaaa
    15841 ggctttcccc atcccgatag gccagttcat agggtagcta gcaatgccca agatttcttc
    15901 caattcttgc aagagatcca aaggctcacg accgtcacgg tccagcttgt tcataaaggt
    15961 aaaaactgga atgccacgat gtttcacaac ctcaaacaat ttcttggttt gagcctcgat
    16021 ccccttggca gagtccacga ccatgaccgc agcatccacc gccatcaagg tacgataggt
    16081 atcttctgag aagtcctcgt gccctggcgt gtctaagata ttcacgcgct tgccgtcgta
    16141 gtcaaattgc ataacagatg aagtaacaga aatcccacgt tgcttctcga tatccatcca
    16201 gtcagattta gcaaaagtcc ctgttttctt cccttttacc gtaccagcct cacgaatctc
    16261 acccccaaag tagagtaact gctcagtgat ggttgttttc cccgcgtccg ggtgggagat
    16321 aatggcaaag gtacgacgtt tcttaatttc ttcttgaata ttcataagtt ctctttcttt
    16381 gattctctat ttttcttgtt tcaatagctg agaatgattt ttacattgga ttttaccatt
    16441 cctttcaaca ctccattata tcggatttta gcattttttt caatttctat ttcttttcac
    16501 ttccccctcc cttatttata ggaaaatatg gtaaaataga acagactaaa aatcatcatt
    16561 tcacgaaagg atgcaagatg aaaattacgc aagaagaggt aacacacgtt gccaatcttt
    16621 caaaattaag attctctgaa gaagaaactg ctgcctttgc gaccaccttg tctaagattg
    16681 ttgacatggt tgaattgctg ggcgaagttg acacaactgg tgtcgcacct actacgacca
    16741 tggctgaccg caagactgta ctccgccctg atgtggccga agaaggaaca gaccgtgatc
    16801 acttgtttaa aaacgtacct gaaaaagaca actactatat caaggtgcca gctatcctag
    16861 acgatggagg agatgcctaa tgacttttaa caataaaact attgaagagt tgcacaatct
    16921 ccttgtctct aaggaaattt ctgcaacaga attgacccaa gcaacacttg aaaatatcaa
    16981 gtctcgtgag gaagccctca attcatttgt caccatcgct gaggagcaag ctcttgttca
    17041 agctaaagcc attgatgaag ctggaattga tgctgacaat gtcctttcag gaattccact
    17101 tgctgttaag gataacatct ctacagacgg tattctcaca actgctgcct caaaaatgct
    17161 ctacaactat gagccaatct ttgatgcgac agctgttgcc aatgcaaaaa ccaagggcat
    17221 gattgtcgtt ggaaagacca acatggacga atttgctatg ggtggttcag gtgaaacttc
    17281 acactacgga gcaactaaaa acgcttggga ccacagcaag gttcctggtg ggtcatcaag
    17341 tggttctgcc gcagctgtag cctcaggaca agttcgcttg tcacttggtt ctgatactgg
    17401 tggttccatc cgccaacctg ctgccttcaa cggaatcgtt ggtctcaaac caacctacgg
    17461 aacagtttca cgtttcggtc tcattgcctt tggtagctca ttagaccaga ttggaccttt
    17521 tgctcctact gttaaggaaa atgccctctt gctcaacgct attgccagcg aagatgctaa
    17581 agactctact tctgctcctg tccgcatcgc cgactttact tcaaaaatcg gccaagacat
    17641 caagggtatg aaaatcgctt tgcctaagga atacctcggt gaaggaatta acccagaggt
    17701 taaggaaacc attctaaatg ccgctaaaca ctttgaaaaa ttgggtgcta ttgtcgaaga
    17761 agtcagcctt cctcactcta aatacggagt tgccgtatac tacatcatcg cttcatcaga
    17821 agcttcatca aacttgcaac gcttcgacgg tatccgttac ggctatcgcg cagaagatgc
    17881 aaccaacctt gatgaaatct atgtaaacag ccgaagccaa ggttttggtg aagaggtaaa
    17941 acgtcgtatc atgctgggta ctttcagtct ttcatcaggt tactatgatg cctactacaa
    18001 aaaggctggt caagtccgta ccctcatcat tcaagatttc gaaaaagtct tcgcggatta
    18061 cgatttgatt ttgggtccaa ctgctccaag tgttgcctat gacttggact cactcaacca
    18121 tgacccagtt gccatgtact tggctgacct cttgaccata cctgtaaact tagcaggtct
    18181 ccctggaatt tcgattcctg ctggattctc tcaaggtcta cctgtcggtc tgcaattgat
    18241 tggtcccaag tactctgagg aaaccattta ccaagctgct gctgcttttg aagcaacaac
    18301 agactaccac aaacaacaac ccgtgatttt tggaggtgac aactaatgaa ctttgaaaca
    18361 gtcatcggac ttgaagtcca cgtagagctc aacaccaatt caaaaatctt ctcacctact
    18421 tctgcccact ttggaaatga ccaaaatgcc aacactaacg tgattgactg gtctttccca
    18481 ggagttctac cagttctcaa taaaggggtt gttgatgccg gtatcaaggc tgctcttgcc
    18541 ctcaacatgg acatccacaa aaagatgcac tttgaccgca agaactactt ctatcctgat
    18601 aaccccaaag cctaccaaat ttctcagttt gatgagccaa tcggttataa cggctggatt
    18661 gaagtcgagc tagaagacgg tacgaccaag aaaatcggta tcgaacgtgc ccacctagag
    18721 gaagacgctg gtaaaaacac ccatggtaca gatggctact cttatgttga cctcaaccgc
    18781 caaggggttc ccttgattga gattgtatct gaggcagata tgcgttctcc tgaagaagcc
    18841 tatgcttatc tgacagccct caaggaagtt atccagtacg ctggcatttc tgacgttaag
    18901 atggaggaag gttcgatgcg tgtggatgcc aacatctccc ttcgtcctta tggtcaagag
    18961 aaattcggta ccaagactga attgaagaac ctcaactcct tctcaaacgt tcgcaagggg
    19021 cttgaatacg aagtccaacg ccaggctgaa attcttcgct caggtggtca aatccgccaa
    19081 gaaacacgcc gttacgatga agcgaataaa gcaaccatcc tcatgcgtgt caaggaaggg
    19141 gctgctgact accgctactt cccagaacca gacctacccc tctttgaaat ttctgacgag
    19201 tggattgagg aaatgcggac tgagttgcca gagtttccaa aagaacgtcg tgcgcgttat
    19261 gtatctgacc ttggtttatc agactacgat gctagtcagt tgactgctaa taaagtcact
    19321 tctgacttct ttgaaaaagc tgttgcccta ggtggtgatg ccaaacaagt ctctaactgg
    19381 ctccaagggg aagtcgctca gttcttgaat gctgaaggta aaacactgga acaaatcgaa
    19441 ttgacaccag aaaacttggt tgaaatgatt accatcatcg aagacggtac tatttcatct
    19501 aagattgcca agaaagtctt tgtccatcta gctaaaaatg gcggtggcgc gcgtgaatac
    19561 gtggaaaaag caggtatggt tcaaatttca gatccagcta tcttgatccc aatcatccac
    19621 caagtctttg ccgataacga agctgctgtt gccgacttca agtcaggcaa acgtaatgca
    19681 gacaaggcct tcacaggatt ccttatgaag gcaaccaaag gccaagccaa cccacaagtt
    19741 gcccttaaac tacttgcaca ggaattggcg aagttgaaag aaaactagac agaacaaaac
    19801 cagccctaag gttggttttt tcttgacaaa tagaaagaaa acgtttgcaa tattgcgtgt
    19861 aaagtttcca aacaagaaaa ctaaaaatcg caacaataag gagttattta tatggctaca
    19921 atgaaggcag ctcgctggca tgcagcgaaa gatgttcgta ttcaagaaat agaagttcct
    19981 gaagtacttc cgcaccaagt aaaagttgca gttaagttca caggaatttg tggtactgac
    20041 ctccatgaat ttttagatgg acctatcttt atcccaacag atgagcacgt ctattctggt
    20101 caaaaagcac cggtaacact gggacatgaa ttttctggtg aaatcataga agtcggaagc
    20161 gatgttaccc gtgttaaagt tggtgatcgt gttgctgtgg aaccaattct agctaaaaat
    20221 aacttagttg gtgattataa tttggatcca aaccttaatt ttgtcggttt ggctgcagac
    20281 ggtggatttg ccaaatattg tgttttagac ggtgacttag tacatgtcat tccagatagt
    20341 ttgagctatg agcaagctgc tcttactgaa cccgctgctg ttgcagtcta tgctgttcgt
    20401 caatcttctt tgaaggctgg cgatacagct gttgtctttg gtttaggccc gattggtcta
    20461 ttaattgtag aagctcttcg tgcagctggt gcttctaaga tttatgctgt tgaactatca
    20521 cctgaacgtc aagcaaaagc tgaagagtta ggagctatcg ttgtccgtcc ggaagaaggt
    20581 gaaacagctg ttgaagctat tcatcgttta acaaacggtg gtgtagatgt ttcttatgaa
    20641 gtaacgggtg tgccagtagt tttaggacaa gccttagctg ccgtacataa agcaggtgag
    20701 tgcatggtgg tctctatctg ggaacgagaa gcaaatatta atccgaatga atttgcaatc
    20761 caagaaaaga cactaaaagg aatcattgct tatcgtcata tcttccctaa agtattagaa
    20821 ttgatggaac aaggctactt ctctgctgaa aaattagtta ctaagaaaat taaattggaa
    20881 aatatcgtcg aagaaggctt tatcgaatta actcaagata agtcacaaat taaaattttg
    20941 gtagaaccag aataaaaaat caatcttttt aagacaatac taacaggtcg gagtaaactt
    21001 acttcggcct tttgaaatat aatactcttc gaaaatctct tcagaccacg tcagcgtcac
    21061 attaccgtat atatggttac tgacttcgtc agttctatcc acaacctcaa aacagtgttt
    21121 tgagcagctt gcggctagct tcctagtttg ctctttgatt tccattgagt atgagaagaa
    21181 atatagtgga tactctttaa cattaaccac tccacctgac tagccaatat tctttttcgc
    21241 tcctaccaac tcccaataac tattttggct ttatttccag agtattttat ggtaaaatga
    21301 agagtaataa tatttattaa agaggtaaaa acatgattga agcaagtaaa ttaaaagctg
    21361 gtatgacctt tgaaacagct gacggcaaat tgattcgcgt tttggaagct agtcaccaca
    21421 aaccaggtaa aggaaacacg atcatgcgta tgaaattgcg tgatgtccgt actggttcta
    21481 catttgacac aagctaccgt ccagaggaaa aatttgaaca agctattatc gagactgtcc
    21541 cagctcaata cttgtacaaa atggatgaca cagcatactt catgaataca gaaacttacg
    21601 accaatacga aatccctgta gtcaatgttg aaaacgaatt actttacatc cttgaaaact
    21661 ctgatgtgaa aatccaattc tacggaactg aagtgatcgg tgtcaccgtt cctactactg
    21721 ttgagttgac agttgctgaa actcaaccat ctatcaaagg tgctactgtt acaggttctg
    21781 gtaaaccagc aacgatggaa actggacttg tcgtaaacgt tccagacttc atcgaagcag
    21841 gacaaaaact cgttatcaac actgcagaag gaacttacgt ttctcgtgcc taatctctag
    21901 aaagaggtca ttctatggga attgaagaac aacttggcga aatcgttatc gccccacgtg
    21961 tacttgaaaa aatcattgct atcgctactg caaaggtaga gggtgttcac tctttttcaa
    22021 acagatcagt gtctgatacc ctttcaaaac tttcactcgg ccgtggcatt tatcttaaaa
    22081 acgtggacga agaactcaca gcagatatct atctctacct tgagtacgga gtaaaagttc
    22141 ctaaggtagc ggttgctatc cagaaagctg tcaaagatgc cgtccgtaat atggctgatg
    22201 tagaactcgc tgctatcaat attcacgttg caggtatcgt cccagataaa acaccaaaac
    22261 cagaattgaa agatctattt gacgaggact tcctcaatga ctagtccact attagaatct
    22321 agacgccaac tccgtaaatg cgcttttcaa gctctcatga gccttgagtt cggtacggat
    22381 gtcgaaactg cttgtcgttt cgtctatact catgatcgtg aagatacgga tgtacaactt
    22441 ccagcctttt tgatagacct cgtttctggt gttcaagcta aaaaggaaga actagataag
    22501 caaatcactc agcatttaaa agcaggttgg accattgaac gcttaacgct cgtggagaga
    22561 aacctccttc gcttgggagt ctttgaaatc acttcatttg acactcctca gctggttgct
    22621 gttaatgaag ctatcgagct tgcaaaggac ttctccgatc aaaaatctgc ccgttttatc
    22681 aatggactgc tcagccagtt tgtaacagaa gaacaataaa aagaaaaagt gtttgatatc
    22741 tatcaaacac tttttctttg cctcccacta tctaaaaaaa tgataataga tataattgta
    22801 aacaaaaatc cagataggtt ttgcatgatt gagaaagtta aaaaaactat ggcagagaat
    22861 cgttaatctc agattgtcgg tagaacgata aacaagggca aaaaagaaac caatcagact
    22921 ataatataat aaactaattg gatctctgtg agatagtatc aaatgactaa gcccaaagat
    22981 aaggcctgat aagatgacat ctaaataata cttggactag ggaaagaagg tattcataaa
    23041 ataccctcta tcaagagtct cctcaaaaac aggaccgatg attacaggca ggacaaaaga
    23101 taagatagtc gataaaaagg ttggttgtcc atttgaaaga accacggtaa aatactcatc
    23161 atgaatattc ctatgattaa tcaaatgagc atagcgtgcc caaaaattac cgagaatctg
    23221 ataaaccaca taagttgcaa ataaggagaa gacaaatgac cagttccagc tctttttctc
    23281 aaagataaag agcatctttt tcttttttaa cctccaaatt aatagaaaag gaaacttccc
    23341 actaatccca ctgttaaaat aagagaatag acatcagctc ctaaccctaa aatgatcgtc
    23401 acatacaatc caattgtttg tggtaaatag gtagatagta aaataataag caaaaatatt
    23461 ccaaattgtc ttagtttctt tgtgtttctc atcgtacttt tttgaaagat taccctgttc
    23521 ggaagccgta cttccaagca tctatataag aattaagtgc cccttgcctc atataaggag
    23581 caaattctct ataatataac catctactat atccatcttc ccaaacagca agaccacctg
    23641 aagtttgctc caagtcctca gttgaaagaa ctgtaaatgt atttgtacct gtcattgcaa
    23701 gtaccttctt aaaatatagt gatttgaaat aagatgtgaa caactctatc aggaaagtca
    23761 aattaattta tagaaatatt ttagcagcca aggtgtactg ttatagattc aatacactat
    23821 aaatttttgt aagcttacat ctacagtata catctttttt gtctatttta tagcccatct
    23881 cctcaactgt caatttttca acctgaattg catttttcca taaaaaatga gacctttcta
    23941 gtctcattta gtcattctta gtattttcta aatcgttgat agcgttcttc cagcaactct
    24001 tctagcggtt tttgtgaaag tctagccagc tccgtttgga gttctttttt gacactctta
    24061 atcagttctt tactagaaag tcctacttca gaaatcacct tatccaccac gtccatttct
    24121 aacagttcat gcgaagtgat tttcatcagt tctgctgctt ccatggcgcg agtaccgtcc
    24181 ttccataaaa tggaagcaaa gccttctgga ctgagaatgg catagataga attttccagc
    24241 atccagacac ggtccgcgac agctagagcc agagccccgc ctgaaccacc ttcaccgata
    24301 ataatggcga taataggaac tttcaggtca ctcatttcca tgagattgcg agcgatagct
    24361 tccccttgac cacgttcttc tgctccgaca ccaggataag cacctgctgt attaataaag
    24421 gtcacaactg gacggccaaa tttctcagcc tgtttcatca accgcagtgc ctttcggtag
    24481 ccttctggat gtggttggcc aaaattccgt ttgaggttgt cttgcaaact cttgcctttt
    24541 tggataccaa caactgttac agcttggtct ccaagccaac caataccacc aacaactgca
    24601 ccatcatcac gaaaagaacg gtcaccatgt aattggataa attcatcaaa aatgcctgtc
    24661 gcaaagtcca aggttgtcaa gcgactctgc tcacgcgctt ctctgactat ttttgcaata
    24721 ttcatctagg actccctcca tgcaatctga ctaggctagc aatcgtatct ggtaagtctc
    24781 ttcttttgac aatagcatcc acaaagccat gttctaatag gaattctgcc ttttggaaat
    24841 cctcaggcaa gctttcacga accgtatttt caatcacacg acgcccagca aaaccaacca
    24901 agctctgtgg ttcagccaga atgatatcgc cttccatagc gaaagaagct gtcacaccac
    24961 cagtcgttgg atctgtcaaa atggtcaggt aaaagagacc agcatttgaa tgacgtttaa
    25021 ccgccgcaga gatcttagcc atctgcatga gactcatgat tccttcctgc atacgggctc
    25081 caccagaggc tgtgaatagg acaactggca atttttcgac agtcgcatac tcaaacaaac
    25141 gagtgatttt ttcacctaca accgtaccca tagaagccat gataaagtta gaatccataa
    25201 tcccaagagc cacagtctga cctttaataa gagcagttcc tgtcacaacg gcttcatgca
    25261 gacctgtttt ttcacgcata gatgccagtt tcttttggta accagggaaa tgcaagggat
    25321 ccttgctttc aatccctgta aacaattctt tgaaggttcc catatcaatc gtcaaagcca
    25381 agcgttcttg ggcagaaata cgaaaggtat agctacagtg cggacagata cgttcacttc
    25441 ccagatcctt ctgatagatg gtatgcttac agcctggaca ctgggaaaat aattcatctg
    25501 gaacctctgg cttagcttga ggtttttccc taaccgaacg attgggattg attcgaatat
    25561 acttatcttt tttactaaat agagccattg attccccttt tcggtttaaa ctcttaaagt
    25621 cattttattc tttttcttga tatttaggta agaaggtttc catcaagaag gaagtatcat
    25681 aatccccagc aatgacattg cgatctgaaa tgaggtcaag ctggaaatct gcattggtct
    25741 gcactccttc aatctctaat tcatagaggg cacgttgcat tttcatcaag gcgtcaaaac
    25801 gattttcgcc gtgtactatg attttggcaa tcatactatc ataataaggc ggaatggtat
    25861 aacctggata aactgctgaa tccacgcgca agccaactcc accacttggc agatagagat
    25921 tagtaatctt acctggactt ggagcaaagt taaaggctgg gttttctgca ttgatacgac
    25981 actcgatggc atgaccgcgt aggacaatat cttcttgctt aacagacaaa ggctgacctg
    26041 ccgcaatgca aatctgttcc ttaacgatat caacacctga aacaaactct gttactggat
    26101 gttctacctg aacacgagta ttcatctcca tgaaatagaa attgctactt gcttcatcaa
    26161 gaagaaattc aatggttcct gcattctcat agccaacaaa ctctgccgct cgaacagcag
    26221 cagcacctat ttcatgacgc agcgtttttc cgattgcaat cgagggactt tcttccaaaa
    26281 ccttttggtt attcctttga agagaacaat cccgctcacc caagtgaatc acatgtccat
    26341 gctcatcacc taggatttga acctcaatgt gccgagctgg atagataacc cgttctatgt
    26401 acatggcacc attgccataa ttggccttgg cctcactaga ggcagtttca aaggcagaaa
    26461 cgaggtcatc tggtttttca accttacgaa tccctttacc acctccacct gctgaagcct
    26521 tgagcataac aggatagcca attttttcag caacaatcaa agcttcttca gagttatgca
    26581 cttctccatc tgaacctggt ataacaggca cacctgcttt aatcatctga gcacgcgcat
    26641 tgatcttatc ccccatcata tccataacat gaccagatgg accgataaac ttgataccta
    26701 cttcttcaca catggtcgca aatttggaat tttcactgag aaatccaaaa ccagggtgaa
    26761 tagcttctgc ctcagtcaag actgcagctg atagaactgc attaatattg agataagact
    26821 ctgttgcctt gccaggacca atacaaactg cttcatctgc caaaagcgta tgaagagctt
    26881 ccttatcagc agttgaataa accgctaccg tcgcaatccc caattcacgt gccgcacgga
    26941 taatacgaac cgcaatttca ccacgattgg caattaaaat ttttcgaaac atggagaacc
    27001 tccttagttc ccaattgcaa aagtaaggat accactggct gcaagcttgc catccacttc
    27061 agcctttgct tcaaccacag ctatggtgcc acgacgtttt acaaaagtcg ctgtcataac
    27121 caattggtcg cctggtacaa cttgcttctt gaacttaacc ttgtccatac cagcgtaaaa
    27181 gaccagtttt cctttatttt caggttttga taactccaac acaccagcag tttgcgccaa
    27241 ggcttccata atcagaacac ctggcataac tgggtattga ggaaagtggc cgttaaagaa
    27301 aggttcgtta atggtcacat ttttgatagc aacaatggta tcctcgctca cttccaagac
    27361 acggtccact agaagcatag gataacggtg gggaagagct tctttgattc cttgaatatc
    27421 gatcatttga tacgtaccaa tcctttacca aactcaacca tttcttcgtt agagacgaga
    27481 atttccgtta ccacaccatc cttaggagct gggatttcat tcatgacttt catggcttcg
    27541 ataattacca atgtttgacc ttttttgaca ctatcaccaa ctgtaacgaa ggcaggttta
    27601 tctggtccag cagccaagta aaccactcca acaagtggac tctctacaag atttccctca
    27661 gtagccacac ttgcttcagc tggagctgga acttcttctg ctacagtctc tgctggagca
    27721 gatgtaggag ctactggact cggtgttgct agaacgggtg ctggagcgac ttgagttgca
    27781 acttcaggca caggtctcgc ttcattcttg ctaaactgca actcatccgt cccattttta
    27841 taagaaaatt ctctcaaact tgactggtca aattgagtca tcaagtcttt aatatcgttt
    27901 aaattcatac ttatctattc tcccaacgtt tgaaagcaag aactgcattg tggcctccaa
    27961 aaccaaaagt atttgaaata gcgtatggaa tttctttctc caagccttgt ccataaacga
    28021 cattagcttc gatataatct gatacttcac ttgtcccagc tgtcattggt acaaagttat
    28081 ggcgcatagc ttcgatggtg acgatagctt ctactgcacc cgcagccccc agcaaatgtc
    28141 ctgtaaaaga cttggttgat gatacaggta cttccttacc aagaacagct acgatagctc
    28201 cactttctcc tttttcattg gcaggagttg acgttccgtg agcattgaca taggctactt
    28261 gctctggaga aatctcagct tcttccaagg ctagtttgat ggccttgata gctccctgac
    28321 cttctggatg tggagaagtc atgtggtagg catcacaagt atttccgtaa ccaaccactt
    28381 cagccaggat agtagctcca cgtttttcag cgtgttcaag actttctaaa accaacatcc
    28441 ctgaaccttc acccataaca aacccattgc gatccttatc aaatgggatc gaagcacgag
    28501 ttggatcctc tgtagtagag agagctgtta aggcttggaa accagcgatg gcaaaaggtg
    28561 tgatagaagc ttctgttcct cccaccaaca tcacatcttg gaaaccaaac ttaatggagc
    28621 ggaaggcatc cccaatcgca tcatttgatg aagagcaggc agtattgata gatttacaaa
    28681 caccgtttgc accaaaacgc atggctacat tcccagaagc catatttggt aaagcttttg
    28741 gaagagtcat tggtttgaca cgtttgggtc ctttttcatg aaggcgaagt acctgatctt
    28801 caatttcctt gattccacca ataccagatg caacgataac accaaaacga tccctattaa
    28861 gagcctctac atcaagattg gcatggttta cagcctcttg ggctgcatac aaggcatata
    28921 aagaatagtt atcaaaacgg ttggtatctt tttttacaaa gtatttatcg aacggaaaat
    28981 cttggatttc tgccgcatta tgcacatcaa agtcactatg atcaaatttt gtaatgccac
    29041 caatgccgat tttcccagtt gctaaactat tccaaaattc ttctggtgta tttccgattg
    29101 gagatgttac tccataacct gttaccacta ctcgatttag tttcattctt ttcacctcta
    29161 gctttcgcta catacttaag ccaccatcaa tggcaatcac ttgaccagtt agataatctt
    29221 ggcctgctaa aaatactgtc aaatctgcaa cctgctctgc ctgcccaaat tctttcatcg
    29281 gaatctgagc tagtgtagct tccttaatct tatctgataa gatagctgtc atatcagact
    29341 caatcattcc tggagcaatc acattgactc gtatattccg actagcgacc tcgcgtgcca
    29401 cagacttggt aaagccaatc aagccagcct tagaagcagc atagttagct tgaccaatat
    29461 tccccatcaa accaacaaca ctagacatat taatgatagc accttctctg gctttcatca
    29521 tcggtttcaa gactgattgt gtcatattaa aggcaccagt cagattgacc ttgagcactt
    29581 tttcaaaatc tgcttctgtc atcttgagca taagagtatc ttgggtaatc cctgcattgt
    29641 tgaccaaaac atctactgaa cccagttctg caatagcttg atcaatcata cgcttagcgt
    29701 ctgcaaaatc tgatacatct cctgaaatgg gaaccacctt gataccatag tttgaaaact
    29761 cagcgagcaa ttcttctgag attgccccac gactgtttaa gacaatgttg gctcctactt
    29821 gagcaaactt gtgggcgatg gcaagaccaa ttccacgact cgaacctgta ataaagatat
    29881 ttttatgttc tagtttcatt tttttccttt caaaacttct acttatttta gtctattttt
    29941 ctaaaagtgc tactaaactc gcttgatctt ccacatgagc taagtgagca gtttgatcaa
    30001 tttttttaac aaaacctgac aagactttcc ccggtccaat ctcgataaag ttgcttatgc
    30061 ctgcttcttg catgacccca atactttcat agaaacgaac gggttccttg acctgacgcg
    30121 tcaagagctg agcaatgtcc tctttttgca tcacagcagc ttctgtattg ccgactaggg
    30181 gacaagtaaa atctgaaaaa cttacctgag ctagagtttc agctagtttc tggctagcag
    30241 gctcaaggag agcggtgtga aagggacctg acaccttaag aggaatcaag cgtttggcac
    30301 ctgcttcttg caaaagttca accgctcgat caactgcaac cacttctcca gcaatgacga
    30361 tttgtgcagg tgtgttatag ttggctggag taaccactcc aagttcagaa gctttttgac
    30421 aggcttcttc aatgacctct actggcgtat tgagaactgc taccatcttg ccagagtcag
    30481 caggagccgc ttcttccata taggctccac gcttagctac caaggcaacc gcatcttcaa
    30541 aatccaaggc gccgcttgcc accaaggcag agtattctcc aagagacaaa ccagcaacca
    30601 tatcaggctg atagcccttt tcttgcaata aacggtagat agcaaccgaa gtcgctagaa
    30661 tggctggttg cgtatagcgg gtctgattga gtttgtcttc ttccgtatcg atgagataac
    30721 gtaaatcata accgagcacc tgactcgctc gatcaatcgt ttctttgaca atcggatact
    30781 gatcatagaa atcccttccc atccctagat actgggcacc ttgaccagca aataaaaagg
    30841 ctgttttagt catttcttac aactcctgcc cagcgagagg cttcttcttg aattttctta
    30901 gcggctccgt aatacaaatc ttttaggatt tcttcagctg tttcttcttt agaaacaagc
    30961 cctgcgattt gacctgccat gacagagcca ccatccacat caccgtgaac aactgctttg
    31021 gctagggcac ctgctcccat ttgttcaaag atttctaaat caggatcttc ctgcttaaag
    31081 gcatcttttt cagccagttc aaaatctcta gtcaactgat ttttaatagc acgaacagca
    31141 tgaccaaagt gctgagctga aatcgtagta tcaatatccc ttgcttttaa aattttctcc
    31201 ttgtagtttg gatgggcatt cgactctttt gcaactacaa accgtgtccc cacctgtaca
    31261 gcctctgcac ctagcataaa gccagccgca gcaccttcac catccgcaat tcctcctgca
    31321 gcaataacag gaatagatat agctgtggct acctgtcgca ccaaggtcat ggttgttaat
    31381 ttaccgatat gccccccagc ttccattcct tctgcaataa cagcgtctgc accgattttt
    31441 tccatgcgtt tagctaaagc gacactagga acaacaggaa taacgattat cccagcttca
    31501 tggaaacgtt ccatatactt gcttggattt cctgctcctg ttgtgacaac tttaacacct
    31561 tcttcaataa cgagatccac gatatcttcc acaaagggag ataagagcat gatgttgacc
    31621 ccaaagggtt tatcagtcaa tgatttgatt ttatcaatat tggccttgac aacttctttc
    31681 ggggcatttc ccccaccgat aattcctaat cctccagcct tggaaacagc ccctgccaaa
    31741 tcaccatcag caacccaggc catccctcct tggaaaatag gatagtcaat cttcaataat
    31801 tctgtaatac gcgttttcat agtgcctcca accttccttg cttacgtaat agttcgattt
    31861 caccataatt tgacagtcaa actattacct aaacaagagg gagtgggttt ctccctactc
    31921 cttctactaa tattctgctt attttgcttg ctcttcaacg taagcaacca agtcaccaac
    31981 tgttttcaag tcattttctg cttcgatttg gatatcaaaa gcatcttcga tttctgagat
    32041 tacttggaac aagtccaatg aatctgcgtc caaatcatca aaagttgatt caagtgttac
    32101 ttctgatgcg tcttttccaa gttcttcaac gataatttct tgtacttttt caaatactgc
    32161 catgatagga ctcctttaaa ataaatagtt tttttataac aatgtgttca ccacatgatt
    32221 acctaaattg taagaatgag cgtgccccag gtcaagcctc caccgaagcc tgatagaaga
    32281 acagtctggc taccatctaa agggatgaga ccttgttcta cacactctga aagtaaaatc
    32341 gggatactgg ctgcactggt attgccatat tccatcatat tggctggaag tttggctcgg
    32401 tcaacaccaa tttttctagc catcttatcc aaaatacggt cattggcttg atgaagtagc
    32461 agataatcca agtctgtcac ctctatagga gattcatcaa tagtctgctt gatagacttg
    32521 gctacatctc gaatggcaaa atcaaagact gtgcgtccat ccatcttcaa aaacgaatct
    32581 gcactttctt gatctgaaaa tggagaatgc aaacctgaat gcccataagt taaacactcg
    32641 ctgcgacttc catcgctatt gagactctca gctaagaaat gctcttgctc gctagcttct
    32701 aacaagacac caccagcacc atctccaaac aacacagctg ttgatcgatc cgaccaatcg
    32761 actgccttag agagggtttc actaccaatc accaagcctt tttgaaagcg accagaagcg
    32821 ataaactttt cagcagttga aagagcaaat acaaatccac tgcaagccgc ggttaagtca
    32881 aaagcaaagg ctttattagc gccaatatta gcttgaacac gagcagctgt agagggcatc
    32941 atcgaatctg gagtaatggt agctaggatg ataaaatcca gttcttttcc tgttattcca
    33001 gcttttgcca tcagtttctt agcaacctct gtagccaaat cactggtaga ttctgttctt
    33061 gaaatatgcc tttgtcgtat tcccgttcga cttgaaatcc actcatcatt ggtatccata
    33121 atctgagcca agtcgtgatt tgtaaccact tgctctggca cataatgagc aacctgactt
    33181 atttttgcaa aagccattat ttcaaatcct ccaaaaattg gtaaagatta gtcaaacctt
    33241 tacccatgac agcaatttct tcctcgctca tgccatcaat aattttttct accatggcct
    33301 tgtggaagcg tttatgcagc ctatgaatca agcgaccctt ctttgtcaaa tgcagatgca
    33361 ccacacgacg atcctgttct gaccgaactc gctcaatgta gcccttacgc tctaaattgt
    33421 tcaaacttgt cgtaacagtt ccaagagtta ccatcaactc ttttgacact tgacttggag
    33481 tcacgtctgg agccttaccg atgacatcaa tcgtatgcat ttctttgatg gagatatcct
    33541 taaaacgact acctctcaag ttcacttcct caattacaag gacattgtta aatatagatg
    33601 ttaaatattc attgattcgt tggtagtcca tttttcatat ccctccttct tcaaaaaact
    33661 tttacaatca aaacatttga cagaaagaat tatatcaaaa ttcaaagaat gatgcaagta
    33721 ttttttattt tcctgtaaat ttaggtcttc ttctctccga atgagctcgc actccttctt
    33781 tgaaatcctc tgtttgagct aaggatttct gtaggttcag ttctaaagta gcgtaaccct
    33841 gccagtcttt aaattggctc tcccaaacca acttcttaat ggcagcatag gaattacttg
    33901 aaacacgtct taatttttta agaagctgtt ctctcgtctt ttcaagtttt tcagcttcag
    33961 aaacgcggta aaccaacccc cattctaggg ctttttctgc cgttaaagcc tctcctgtca
    34021 tagctaattg agcagcacgc gtcacaccga tactgcggct caagagatga attccccctg
    34081 catctggagc caaaccaaca ccaacaaagg cttggataaa cttagcctta tccgtcgcca
    34141 aacagaaatc tgccgcaaca gccatattcg ctgcggcacc tgcaacagca ccgtcaactt
    34201 ccattaaaac aggtttggct atttgcttga ttttataaga aatcgtattg accaactctg
    34261 cgatttttgt caatgatgga atatcatcct catccactgc ccgcttcatc tctaccaaat
    34321 ctcccccaac tgagaagacc tttccattgg cattgattaa gataaaatgc acagctggat
    34381 tttcttctgc caaagtcaga gcttctaaaa tctcctcaca catgggaata tgaaaaccat
    34441 ttgcgacctc aggacggttc aaggtaagga ttgccaagtc ctcttcaagc tgataaataa
    34501 tgtgttccat cagggctcct ttttatttta taagcagttt atactcttcg aaaatctctt
    34561 caaaccgcgt cagcttccat ctgcaacctc aaagcagtgc tttgagctga cttcgtcagt
    34621 cttatttaca acctcaaagc agtgctttga gcagtctgcg gctagcttcc tagtttgctc
    34681 tttgatttca ttgagtgtta aattatttca ctgtcaaagt atatcttttt taggagcaga
    34741 aagcaagaaa aaatcgactg aaagtgtctc agccgataag aaaggctcgc tttaggctag
    34801 aaagcatctt attttcttca ttgaaaaaag cctttctttc tgctataatc gtataaaata
    34861 cttactttag gagttcttat gaaagttgtt aaatttggag gtagttctct tgcctctgct
    34921 agtcaattag aaaaagtttt aaacatcgtc aaaagcgatt cagagcgtcg ttttgtagtc
    34981 gtttctgcgc ctggtaaacg caatgctgaa gatactaagg ttacggatgc cctgattaaa
    35041 tactaccgcg actatgttgc gggtaacgat attagcaaga gccaaagctg gattatcgac
    35101 cgctatgctg ctatggttag tgaattggga ctaaaaccag ctgtgctaga aaaaatttct
    35161 aaaagcattc acgccttggc cactcttcct attgaagaaa atgaatttct ctacgatact
    35221 ttcctagcag ccggtgaaaa taacaatgcc aaattgattg ctgcctactt taaccaaaat
    35281 ggtatcgatg cacgctatat gcaccctaga gaagctggga ttgtggtcac aagtgaacct
    35341 ggtcacgctc gcatcattcc atcaagttat gacaagattg aagaattgac aaacaccaat
    35401 gaagtccttg tcattcctgg tttctttggt gtcactaagg aaaatcaaat ctgtactttc
    35461 tcacgtggag gatctgatat tacaggttct atcattgctg ctggtgtcaa agctgacctc
    35521 tatgaaaact ttacggacgt tgatggtatc tttgcagccc accctggtat tatccaccaa
    35581 ccacactcga ttcctgagtt gacctaccgt gaaatgcgcg agttggccta tgcaggcttc
    35641 tcagtccttc atgacgaggc tcttcttcct gcctaccgtg gaaaaattcc tctggttatc
    35701 aagaatacca acaaccctga ccatccaggt actcgtatcg ttctaaaaca cagtaatgat
    35761 gaatttccag ttgtgggaat tgctggtgac tcaggctttg tcagcattaa tatgtcgaaa
    35821 tacctcatga accgtgagat tggatttggc cgcaaggttc tgcaaatcct ggaagaactt
    35881 aacatcggtt gggaacatat gccaacaggt atcgacgatc tttctatcat tctccgttct
    35941 cgccaactaa ctcccatcaa ggaagaagaa atcctgcgtc agttggttca aaaggctaaa
    36001 gtagatcatg cagaaatcga acacgacctt tctatcatta tgattgttgg tgaaaaaatg
    36061 aagagccata tcggagtgac tgctactgcg acacgcgctc tatctgaaaa caaaatcaac
    36121 atccagatga tgtctcaagg ttctagtgaa gtttctatta tgtttgttgt caataaagac
    36181 caagagaaag cagcaattaa agccctctac aatgcctttt ttggagaaag taaggaagac
    36241 taagcatggc ccaatcactt aacaaaacag tactcctcag tacgacaggc acttcctacc
    36301 tctctatcgc tgggaaagtt gggaaattcc ttgtcggaga tcaggctttg gaattttacc
    36361 cagatgtcaa tgtcgaacaa tttatccaga ttccttggag ccatatcaat cagattggag
    36421 ccaatgtcac tggacgcaaa atcagccgcc acttcgaagt ctttacagac aaaggaaaat
    36481 tcctctttgc ttcaaaagac tcaggtgcca ttctcaaaat tgcacgcgaa aaactaggca
    36541 atgacaaggt cgtcaaactt ccgaccctga ttcagacaat tagccaaaaa tttaaaaatc
    36601 tatttgcaaa aaagtagaaa ctttagtata atcatagcaa cggataagta ggttatcttc
    36661 ttctttcaga aagtctgcgg gtgctgcgag cagataggag ggataggtga aattctaccg
    36721 ttagtgacaa ttacatacac aatcaagtgc acacctgtga agttggatgg aaccgtggcc
    36781 ctgccactcc aacgttttgt cagggtgctt ttttcataaa ggagttctta tgttagatat
    36841 caaacgtatt cgtacagatt ttgaagctgt cgcagaaaaa ttagctacac gtggtgtaga
    36901 tgctgctgtc ttgaatgaaa tgaaagaaat cgatgctaaa cgtcgtaaca tcttggtcaa
    36961 ggttgaaact ctcaaagcag aacgtaacac agtttctgct gagattgccc aagctaagcg
    37021 caacaaggaa aatacagatg acaagattgc tgccatgcaa aatctatctg ctgaggttaa
    37081 agccttggat gctgaattgg cagaaatcga tgctaaattg acagaattta caacgactct
    37141 tccaaatatc ccagctgaca gcgttcctgt tggggctgac gaagacgaca atgtggaagt
    37201 tcgccgttgg ggtactccac gcgagtttga cttcgaacct aaagctcact gggatctcgg
    37261 tgaagacctt ggtatccttg actgggaacg cggtggtaag gtaacaggcg ctcgcttcct
    37321 cttctataaa ggcctcggtg ctcgtttgga acgtgctatc tacaacttta tgttggatga
    37381 acatggaaaa gaaggctata ctgaagtcat cacaccttac atagtcaacc atgattctat
    37441 gtttggtact ggtcagtatc caaaatttaa ggaagatact tttgaactca gcgataccaa
    37501 ctttgtcttg attccaactg ctgaagttcc tctgacaaac tactaccgtg atgaaatctt
    37561 agacggcaaa gatcttccaa tctacttcac tgctatgagt ccgtcattcc gttctgaggc
    37621 tggttctgcc ggtcgtgata cgcgtggctt gatccgtttg caccaattcc acaaggttga
    37681 aatggtcaaa tttgccaaac cagaagaatc ttacgaagaa ttggaaaaaa tgacagccaa
    37741 cgctgaaaac attcttcaaa aactcaacct tccataccgt gtcgttgctc tctctactgg
    37801 agatatgggc ttctcagcta cgaagactta cgacttggaa gtgtggattc cagcacaaaa
    37861 caattaccgt gaaatctcaa gctgttcaaa cacagaagat ttccaagccc gtcgtgccca
    37921 aatccgttac cgtgatgaag cagatggcaa ggtgaaactc cttcatacct tgaacggttc
    37981 tggacttgca gttggacgta cagtggctgc aattcttgaa aattaccaaa atgaagatgg
    38041 ttctgtgacc atcccagaag cacttcgtcc atacatgggt ggagctgaag tcatcaaacc
    38101 ataaaaaata aggtttagct atttctagct agaccttttt tcgtaaccaa atcagataag
    38161 cacctagtac acagaataaa atagttaggc atataatggt ttcagccaat accaggtaat
    38221 ccagaaatgg aagtttcaaa attccctgag ccatcttgag cgaggtcgct gtgataatgg
    38281 ttgggaaggt gagggctgag aaggctggtt gaaaaccttg ttttaaaatg ttgggcagac
    38341 gagttaaaac aaagaaaaag aaggattgag aagccaaaat catgacaatc aagacccaag
    38401 tcggcaggct ggttcctcct actcgaacta gagaagccaa gagtagagag aaaggagcac
    38461 agtagattcc ttcttgtcca agcaaggcta gtgggagtgg atgtttcttt aaatcgctat
    38521 aaataagggg atagagatag aaggtcaaga gaaaaccaaa actcaaggtc gcataggcaa
    38581 tttcgataat acctaccaga ggataggtca aggcagccac tgctatcccc acatagagaa
    38641 ccgtccaact tggagtggca tgaaccctcc gccctggaca agcaaacttg atggtaaaac
    38701 cagcaatcaa ggtcaaatcc aagagaaatg aaaaccacca aatcccttgt gctaccaaag
    38761 gaagataaga gaatacgcga aagacatagg tcgataaaat catcccagcc ataggaaagg
    38821 ttgccattcc tgacaaaaga gggggcttgg tcaattcttg cttggtttct ttccaattaa
    38881 agagatgcag aattagaaag taaatccata aaaccaaacc agtcagacta aaaagatggg
    38941 atagaaccgg caacgtatct aaaataagat ttccagctcc tgccaaacct agcaaacaac
    39001 ctgaaaatac taaggggagt tttttcatcc taacctccaa taatcatgtt agtttcagta
    39061 taacataaaa gcgcttaaat gaggatttaa aaaaacgaag tccgcttatt tcagacttca
    39121 ttttactcag atatgaatta ggcataaggt tgcaattctg gattaattgg tgtattagct
    39181 aagttgttgg catagttaca gaggattgct aggctgacac caaaaaccac atccaaggca
    39241 ttttgttgag tgtagccagc ttctaaaaac tcagacaagg cttcatctcc tacacgaccc
    39301 ttggtattga taactgccaa ggtaaactta gctagggtat ccaatttagg atctgtttca
    39361 attggagtac gattgcgaag agcttgaatc aagtcatcat tcatctggat ttgtttgatg
    39421 gaaaaggctg tgtgacctgc gacacagaag gcacaaccat tggtcacggc tgccgtgatt
    39481 tgcaccactt cacgctcaac gggtgtcagg ctgttgcgac ggtggataga tgagacaatt
    39541 tggtaggctt ctaaaacagt cggggcattg gccaagagac cgattaggtt gggaatatag
    39601 ccattgttgt ctttttctac tgtttcaaga atttctttca cttctgctgg tgctgactct
    39661 actgtatgga tagtaaatgt tgtcataaga tacctctttt cttattattg acactaatat
    39721 tattggaaaa tcttataaaa tcctgattcc taagtttatc taagataaag ctttattctc
    39781 tcataagatt ttcgttgtta tattagttta tcacacttcc aatcacttgc ataatatata
    39841 ttatatatca ggctgataaa aattatttat aggcaaaaaa atcacacgag ctgtgtgatt
    39901 ccattatttg tcaaaatact ttttagtttc agcaataacg actggcgaca agaccaagag
    39961 ggcaatcaag tttggcagaa ccatcaaggc attaacgata tctgcgataa tccagaccat
    40021 atccaactcg ataaatcctc ctaacaagac catgagcaca aaaaccacac ggtagagcca
    40081 gataaagcga accccaaaga ggaactcaaa acagcgttct ccgtaatagt tccaacctag
    40141 aatcgttgta aaggcaaaaa gtacaaggaa gatggtcaag agggcaggcc caaagtgtga
    40201 aaagactgtt gagaaagctg actgagtcaa ggcaacccca ttcaagtcac cactccaaac
    40261 tccagttacc aagatggtca aaccagttag agtacaaatg atgagggtat caataaaggt
    40321 tcctgtcatg gaaatcaaac cttgctctac tggttcattt gtcttggcag ctgcagctgc
    40381 aataggagca gaacccagac cagattcgtt tgagaacaca ccacgcgcca caccattttg
    40441 aatagccatc cgaacgctag caccagcaaa tccacctacc gcagcaaggg gactaaaagc
    40501 tgaggtaaag actaaagcga ttgtgccagg gatttttccg atattaaaga aaataactgt
    40561 aagagttcct aagatataaa tgatggccat aaaaggaaca acagtagttg aaaccttaga
    40621 aatagacttg agtccaccaa agactgcaat cgctacaaag acagacaaga cgagagctgt
    40681 gatggctggc gaaatcgtcg ttgtattttg gatagattct gcaatcgagt tgacttgggt
    40741 gaaggttccg attcccaaga gagcaaccaa tactcctgct actgcaaaca aaacagcaag
    40801 tggtcgccac ttttctccca tccctagaag gatatagtgc atgggacctc ccgctactgc
    40861 accatggtcg tccttggtgc ggtatttgat ggccaagagt ccttccgcat acttggtagc
    40921 cattccaaag aaagccgcca tccacatcca aaatagagct cctggtccac caaccttgat
    40981 agccgtcgca actcctatga tatttcctgt tccaacagtt gatgccaagg ctgtacacag
    41041 agctgcaaaa ctggatacat caccatgtcc cttatcctgg ataaaaataa gctgaaaggc
    41101 cttgggcaga cgcaaaacct gcaagagtcc tagccgcatg gttaggtaaa tccctgttcc
    41161 gaccaataaa atcaagaggg gcggtcccca agcaaaagca tcgattgatt taagcaattc
    41221 taacattttc ttctcctatc gtttcaaccc caaaagaaag agcacatgca agatacatgt
    41281 actctggaat gcttagataa atgctaaaaa gcggtctatc ctagctctgt ccttttacct
    41341 gagagtttga gcagttgcct gccttgcccc ttcggtgcct ttacggtctc tccagagttc
    41401 cgtccattta cagtcatgga aaatcaaacg attccccact tctattaaac ttcattcggt
    41461 gttggtattt aattgattct aattttacaa aaaatgttgg cttttgtcaa tgtgtttatt
    41521 agtaaaaatt agttcaacag tttttacttt ataaagtcca gaatactgct atcctttaaa
    41581 agtgacaata gtcgcaccac tgcctccagc attttgtggg gcatagccga aactcttgac
    41641 atgtttgttt ctttgcaagt atttggtaac tccttcacgg atgactcctg ttccgatacc
    41701 atggatgata tcaacttgag ccatattgtt aagcaaggct tggtcgatga aggtatctag
    41761 ctcattcatg gcttcttcat agcgcttgcc tcgaagatcc agtctagctt gaggtcctcg
    41821 cccagaagtt cgtttcacaa cattgacctg tttcttcttg actggttttt cttgctgggc
    41881 ttgaacaaga tcaaactctt tctcttccaa ggtcatctta atcaagccaa cttgggcttc
    41941 ccagcgaccg tccttgagtt gactggtcaa ggtaccacgc tgaccataac tgagaaccac
    42001 gatatcatct cccacctttg gagctcgttt tttcttggcc ttttgaagga ccttattttt
    42061 agacaagtcc actttttcag gagccaattt tttcaacttg gccttggctt caatgatttc
    42121 gtggggcttg agttgggatt tactgtggag atttttgaga atctggtcac tttcacttag
    42181 ggccatatcc acaatctcag cagcctgttc acgcgccttg ttaagctcgg tttccttttc
    42241 acgattaagc tcgttgtaga gtttttttag cgcacggttc atcttgagat tttcttgctc
    42301 cacctcacgg atattgtcca agcgtttgcg gctttccagc gtctgctctt ctaattgctc
    42361 aatgatacga ttgacgtcat tgtcctgatc gatctgctga ctggcatctc ctacgataac
    42421 ttcagatagg cctagacgtt tggcaatttc aaaggcatta cttcggccag gaacaccctg
    42481 cataaagcga taggtcgggc gaagagttgc agtatcaaac tccatactgg cattttgcac
    42541 aaaggctgtc tcaataccgt aggccttgag ttctggatag tgggtcgtcg ccatggtctt
    42601 gatttgacgc aggcgaaggt cctccagaat agccatggca agggctgctc cctcttgggg
    42661 atcagtacca gcccccaact catccaaaag taagagtgaa tgttggttga ccttgccaag
    42721 aatatccacg atattggtca tatggctaga gaaggtagac aagctctgct caatagactg
    42781 ctcatctcca atatcagcaa agatttcttc aaaaatacca acacgacttc ccttgtctgc
    42841 taaaatcggc aatcctgact gggccatgac ctgtgtcaag cccagagttt tgagcatgat
    42901 ggtcttccca cctgtattgg gacctgtaat gacaatagct gttaaatctt gaccaaaata
    42961 gacatcattt gcgacggcat ttttgaccaa aggatggcag acatggagca gttgaatctc
    43021 ttgattttct gacagctgag gcacgactgc ttgtctttct tggataaatc gaaccttggc
    43081 acgaatcaag tccagatgac cgataatcca agcgtcatta gcaatctcag ccgcatgagg
    43141 gcggacacgc tcagaaattt cttggagaat gcgaagcatt tcatagcgct catctgctcg
    43201 cagactagca atttcttcgc tcagtttgac tacctcacgt ggttcgatat agacggtgtt
    43261 tccactagca gaaatatcat gaacgacacc tgcaatctta ttgcggtagg tgtttttgac
    43321 tggtaaaacc tgacggccat ttctgctagc aacaattcct tccgtcaaca tctgcgcttt
    43381 ttgcttgagc aagtcttgta aaacatcgcg tacctgactc tcgctatcat gtatttttcg
    43441 acggattcgc gccaattctt cactggcaaa attttcaatg aaacccgcat cattaaaggc
    43501 ctgaagattt ccttgtaatt gcggaaaatc atgtaatttc tcaaaccaaa gggctaattc
    43561 ttccaagctg acattttcca gattggtgta aaaattttga agttctcggc tggcaagaag
    43621 cacgcgtttc aagagtagga actcctcgat attgagatcc gctcccatct ccaacctctt
    43681 gcagactcct gcaatttcct tagttgagag aatagtaaaa tgcggttgct cgacgaaaag
    43741 agcctgcatt tccttcatct cagcaaaagc ctgtttgatt ttatctgctt tggcagtcgg
    43801 agccagttgt ctcaattgct ccaagccctg ctcggtcaac aaatgaggct caaacaaggc
    43861 cttgacctta tcgaactcta atgtttctaa tattttctta ttcatcttta tttccttgtc
    43921 tttgaatgtc taggtgtggt agctttttac attctgatag attcgagtca aatctgtaaa
    43981 caaagggcta ggaaaactcc tgcccttttt atccgattaa atttgtcacc cagatttgtt
    44041 tgagccaact ggttgttatc ggtatgctct ggatgatgtg ttttgcgacg atactctttt
    44101 caagaggatt ttgtataact gccatgggga tggtcgccaa gattgtcaag gccatttgca
    44161 agacaaataa ggtcaccaac atggacaaga tacctgctga aacttggaac aacttaccac
    44221 ccagtttttt gctaggaatc aagtgtaaga gaagaccaag caaacgacca atgctataga
    44281 caatcccaaa tacaagcaag tagccgatac ctgcataaaa gaccttatcc agctgaaaga
    44341 gttgatccga tgggaaaaag aaagtcccct gaccttcctg cgaatttgca taagggagca
    44401 ataaatggaa ttgctctcca agccccttat aaaactggcc agccataaaa gccgatgcca
    44461 tggctgaaat caggtaataa acctgtaaga gcaggcctct ccgatagccg atataaaatc
    44521 cccaaaccaa gaccaataga agaaggaatg aaatcataag gaatcctcaa tcttgctctg
    44581 ttcttgctta caagtcacaa gcttgtgacg gagttcttct agctcttgct ccttatcgtc
    44641 aaattcaatc tcacggctga gctgagttga taaacagttg actgccaaca aaagagcgat
    44701 tgtttcatca tctgcgctag gcatttgttc tttaattgct tggtattttt ctgtcgcaac
    44761 cttagcgatt tcctccataa aaaggttatc atgttcgctt gtcaaggtta acgatttttt
    44821 cccgaatgta aatttgaatc gatttagatt tgccataaaa ttcacctcac gatattatac
    44881 caaaattcgc taattttgtc agtttttaca aattttactg cttttgtggt acaatagaaa
    44941 ctatggcaag tatcacactc acaccaagcg aaaaggatat tcaggctttt cttgaacact
    45001 atcaaaccag tctggctccc agtaagaatc cctatatccg ctactttttg aaactacctc
    45061 aagcaacggt ttctatctat acttctggaa aaatcttgct tcagggtgaa ggggctgaga
    45121 aatacgctag tttctttggc tatcaacttg tagaggaaaa cagggggcaa aatcttcctt
    45181 tgattgggac agatgaggta gggaatggtt cctactttgg tgggcttgca gttgtggctt
    45241 cctttgtcac acctgaccag catgccttct tacgaaaact tggtgtggga gattctaaga
    45301 ctctgaccga ccaaaagatt cgtcagattg ctcctattct caaggaaaaa atccagcacc
    45361 aggcactcct tctctcaccc agcaagtaca acgaggtcat cggagaccgc tacaatgctg
    45421 tttcggttaa ggttgccctc cataatcagg ctatctatct cctccttcaa aaaggtgttc
    45481 agcctgagaa aattgtgatt gatgccttta ccagtgctaa aaattatgac aagtacttgg
    45541 cacaagaagc caattgtttc agcaatccta tcagcttaga agaaaaggct gagggcaaat
    45601 acttggctgt cgcagtttct tctgtcattg cgcgtgatct ctttctggaa aatcttgaaa
    45661 atttgggacg agaactgggt tatcagctcc caagtggagc tggaacggct tctgacaagg
    45721 tggctagcca gattttgcaa gcctatggta tgcagggact cagcttctgc gccaaattgc
    45781 actttaaaaa cactgaaaaa gcgaaaaaac gcttagaaag gtaagttatg aattcattta
    45841 aaaatttctt aaaagagtgg ggattgttcc tcctaattct gtcattacta gctttaagtc
    45901 gtatcttttt ttggagcaat gttcgcgtag aaggacattc catggatccg accctagcgg
    45961 atggcgaaat tctcttcgtt gtaaaacacc ttcctattga ccgttttgat atcgtggttg
    46021 cccatgagga agatggcaat aaggacatcg tcaagcgcgt gattggaatg cctggcgaca
    46081 ccattcgtta cgaaaatgat aaactttaca tcaatgacaa agaaacggac gagccttatc
    46141 tagcagacta tatcaaacgc ttcaaggatg acaaactcca aagcacttac tcaggcaagg
    46201 gctttgaagg aaataaagga actttcttta gaagtatcgc tcaaaaagcc caagccttca
    46261 cagttgatgt caactacaac accaacttta gctttactgt tccagaagga gaataccttc
    46321 tcctcggaga tgaccgcttg gtttcgagcg acagccgcca cgtaggtacc ttcaaagcaa
    46381 aagatatcac aggggaagct aaattccgct tctggccaat cacccgtatc ggaacatttt
    46441 aagagaccta agaggccgag aatcaccaat ctcagcctct tcttctatcg tgagaagtga
    46501 ttgtttattg acaaatactc aatgaaaatc aaagaacaaa ctaggaagct agccgtaggc
    46561 tgcttaaaac agtgttttga ggttgtggat agaactgacg aagtcagctc aaaatactgt
    46621 tttgaggttg cagatggaag ctgacgtggt ttgaagatat tttcgaagag tataaaataa
    46681 aaacaatccc aactctcacc tattcatgca aaggaatttt atggaagttt atttttcagg
    46741 aaccattgaa cggattattt ttgaaaatcc cagcaatttt tatcgcatcc tcctcctaga
    46801 aatcgacgat acggacgcag aggattttga tgactttgaa atcattgtca caggaaccat
    46861 ggctgatgta attgagggcg aagactatac tttttggggg caaattgtcc agcactccaa
    46921 gtatggagaa caactgcaaa tcagtcgtta tgatcgcgca aaaccaacta gtaagggctt
    46981 ggtcaagtac ttttcaagta gccatttcaa ggggattggt ctcaagacag ctcagaaaat
    47041 cgtggatacc tatggcgaaa ataccattga cgaaattttg caacacccag aaaagttaga
    47101 aggcatcgca ggactctctg ccaaaaatcg cgaggctttc gtctccactc tccgtctcaa
    47161 ctacggaacg gagatggttt tggccaaact agccaactac ggcattccca acaaactagc
    47221 ctttcagatt caagactttt acaaggaaga aacccttgat gtggttgaaa attatcccta
    47281 ccagctggtt gaggatatca agggtttggg ctttaccatt gctgaccaac tagctgagga
    47341 actaggcatc gaaagtcagg ctcctgaacg cttccgcgcc ggtctagttc acagtctttt
    47401 tcaggcctgt atggaaacag gggacaccta tgttgaagca cgggatttgc tggaacaaac
    47461 ccttactctc cttgagtctt cccgtcccgt agaactagac cccagccaag ttgcccaaga
    47521 gctctcctac ctgatcgaag aagacaaggt tcagcagatt gataccaaga tctttgacaa
    47581 cagcctcttt ttcgctgagg aaggcatccg cagtcacttg gttcgtatcc ttgaaaaagg
    47641 aaaacagaag agtcatgatt tagaaaccat tcaaaaacat atcactacta tcgaggaaga
    47701 gctgggaatt gagtatgata acattcaaaa acaggctatc tgtgatgcta tccagaataa
    47761 ggtctttatc ctgacaggtg gacctggtac tggtaagaca actgttatca atgggatcat
    47821 cgctgtttac gccctgttag agggacttga cctcaggaag aaaagcaatc tgcccattct
    47881 tcttgctgct ccaactggac gagccgctcg ccgcatgaat gaattgacag gtttgcctag
    47941 cgcgaccata caccgccact tgggaatgac aggtgacgat gataccagtc atctggaaga
    48001 ttacctagat gctgacttta tcatcgtgga tgaattctct atggtggata cttggctggc
    48061 caatcaactc ttctccaata tctcttctaa cagtaagatc ctcatcgtag gtgacagtga
    48121 tcagctacct tctgtcagtc ctggacaggt tctagcggat ctgcttcata ttcctttgat
    48181 tcctcagact cgcttggaaa aaatttatcg acaaagcgaa gaatcaacca tcgtcaccct
    48241 agctagtcag attcgacagg gcatcttgcc agctgatttc acccaaaaaa aagctgaccg
    48301 ttcctacttt gaaattgcta gtggccatat tcctgccacc attgaaaaaa tcttaggtgc
    48361 cgccctcaga aatggtattc ctgcccgtga tatccaagtt ctggctccta tgtaccgagg
    48421 gacggcaggg attgatgcta tcaatcagct catgcaagac ctgctcaatc ccccacaaaa
    48481 agatcaactc agttttgaag ctcctcagtg ccactatcgt aagggagaca aggtcattca
    48541 tttggttaac gatgctgaaa tcaatgtctt taatggggat ttaggagcta tcacagacct
    48601 gattcctggt aaatacaccg aatcgaaaca agacgagatt gtcattgatt ttgatggtaa
    48661 tgaagtctct tacccccgta acgaatggta caagattcgc ttggcctatg ccatgagtat
    48721 tcataagtct cagggaagtg agttcccagt tgtcatccta ccgattacta gtgctagccg
    48781 acgtatgctg gagcgaaatc tcatctacac agccattaca cgtgccaaaa gcaaactcat
    48841 cttactaggc gaattacaag ccttcgacta tgctacccaa cacatcggaa ctgcccgaaa
    48901 aacctatctg attgaacgct tcagtgatct actggagaat gttgaagaaa agcaacaagc
    48961 tgtctctgaa acagtcacat caagtgcctc tgaacaatcc tacatcctaa ccgaagaaaa
    49021 ctgggacagc attccagcca tgattgggat tacagacaca gacctcaaag agatttttgg
    49081 aaaatagtac atcagaaaac ccaccaattc aggtgggttt tctgtttatt aagattattt
    49141 tactgttgct gtgcttgtaa tcaattcaac agcttttttg attgtgatat catgtttcaa
    49201 catgtcagct gaaagcaagc tttgtacttg agcaacttcc atgttgtagt ctgctgccaa
    49261 ttgctcaact tctttttgga tttcttcttc tgaagcatca aatccttcag ctttggcaac
    49321 tgcttcgata acaaggttag tcttagtacg tgactcagct tctgcttggt attggttgtg
    49381 aaggtcttct tgagtagttc cagtgatttg gaagtacatg tcagggttga tcccttgacg
    49441 ttgcaagttc ccaaggaatt catttactga acggtgaact tcttcatgga tcatttcttc
    49501 tggaagttct acgatttcag cgttttctac agctgtatca attgctgcac cttcaactgc
    49561 atctttgtaa gcttcttctt tagcagcagc caattctttg cggtattttt ctttcaagtc
    49621 agcaagtgtt tcaacttctt catcaatgtc ttttgcaagt tcatcgtcaa gagctggaac
    49681 ttctttagct tttacttcgt ggatagttgt cacgaattta gcttctttac ctgcaaggtc
    49741 ttctgcttgg tagtcttctg ggaatgttac gataacatca acggtttcgc cagctgagtg
    49801 acctaccaat tggtcttcga aaccagggat gaattgacct gaaccaagtc caagtgagaa
    49861 gttttcacct tttccaccgt caaattcaac accgtcgata gaaccaacga agtcgatcac
    49921 aacagtgtcg ccgttttcag cagcagcttc cttgataacc aattcagcca ggttgttgcg
    49981 ttcgcgttcg atacgctctt caacatcagc gtcagttact tctttttcta catcaactga
    50041 tacttcaagg tttttgtagt cacccaattt tacttcaggt tttgtaacga cttcagcagc
    50101 gataacccag tcttgacctt tttccattga agttacgtca atttttggtt gggcaaccac
    50161 ttcaagacca gcttctttta cagctgcttc ataagcgttt ggcaaaagag cgttcattgc
    50221 atcttgataa agagcttctt caccaaattt ttggtcgaag atagggcgtg gaaggtgacc
    50281 tttacggaaa cctggaacat taagagattt cttcactgac ttgaagacac ggtccaattc
    50341 tggtttgatt tggtcttgag agatagtgaa agtcaagaca ccacggtttg tttctttgtt
    50401 ttcaaatgat acagacattc tgtcatttct ccttaaaatt ttttaaatac agtctattat
    50461 aacattttct ataaataaaa tctagactaa tgcagccaat tatagacaac aagccttttt
    50521 cctaactaaa acactcataa agtatagaca attttagact actttggata tcttttggtt
    50581 agtcattttc caaaactaat ttatctaatt tttcagttat atagta
//
LOCUS       DBERDK010000005        49767 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.005, whole genome shotgun sequence.
ACCESSION   DBERDK010000005 DBERDK010000000
VERSION     DBERDK010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 49767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49767
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.005"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            144..1163
                     /gene="galE"
                     /locus_tag="ACL73L_000266"
     CDS             144..1163
                     /gene="galE"
                     /locus_tag="ACL73L_000266"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HGS0771317.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVERITGVEIPFYEADIRDTATLRDIFKQEEPTGVIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKAMEENNCKNIIFSSSATVYGDPHTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            1165..2118
                     /locus_tag="ACL73L_000267"
     CDS             1165..2118
                     /locus_tag="ACL73L_000267"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGS0771318.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAVHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRTSREGEPFFRSFCAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTYYLDYPNVERQAGKTSWSFRQLFFYSIE
                     GIVNFSDFPLTIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSDLPDFTEKIK"
     gene            complement(2180..2386)
                     /locus_tag="ACL73L_000268"
     CDS             complement(2180..2386)
                     /locus_tag="ACL73L_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HGS0771319.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDDPDQLEKEI
                     SPSQDVLEAVKNCPTRALIGNQEA"
     gene            2438..2914
                     /locus_tag="ACL73L_000269"
     CDS             2438..2914
                     /locus_tag="ACL73L_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HGS0771320.1"
                     /translation="MAKEPWQEDIYDQEESRAGHRHRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKDFHDSDASVVQISSSSSSQPEQSSEPESTSSSS
                     EEAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMATGSWFANPGDVIKIK"
     gene            2923..3594
                     /gene="cmk"
                     /locus_tag="ACL73L_000270"
     CDS             2923..3594
                     /gene="cmk"
                     /locus_tag="ACL73L_000270"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HGS0771321.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLGVEEVEALLALLDQHPISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPEVREKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIAKGIETDLETLKKEIAARDYKDSHRETSPLKQAEDAVYLDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            3725..4063
                     /locus_tag="ACL73L_000271"
     CDS             3725..4063
                     /locus_tag="ACL73L_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HGS0771322.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLTDGDTVTLIKDLKVKGAPKDLKQGTRVKNIRIVEGDHNIDCKIDGFGAM
                     KLKSEFVRKI"
     gene            4066..4695
                     /locus_tag="ACL73L_000272"
     CDS             4066..4695
                     /locus_tag="ACL73L_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HGS0771323.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITRHGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMSRVGENWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWDPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
     gene            4935..6086
                     /locus_tag="ACL73L_000273"
     CDS             4935..6086
                     /locus_tag="ACL73L_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGS0771324.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGTRLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWTAI
                     FLAYSAGFLVLPLYLLFVPYGKSKKEVKKRAKEASRLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
     gene            6399..7148
                     /gene="truA"
                     /locus_tag="ACL73L_000274"
     CDS             6399..7148
                     /gene="truA"
                     /locus_tag="ACL73L_000274"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HGS0771325.1"
                     /translation="MTRYKATISYDGYAFAGFQRQSHARSVQEEIEKTLTRLNKGQTI
                     TVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVADDFHC
                     RYAKHSKTYEFIVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IDLILEKKDRQLAGPTAAPNGLYLKEIRYEE"
     gene            7138..7911
                     /locus_tag="ACL73L_000275"
     CDS             7138..7911
                     /locus_tag="ACL73L_000275"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HGS0771326.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVL
                     DPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASH
                     LIKGDKLLPAVESSKAFVYRAIAQADQYGVRQYEANKNN"
     gene            7889..8350
                     /locus_tag="ACL73L_000276"
     CDS             7889..8350
                     /locus_tag="ACL73L_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HGS0771327.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKIPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLIDLLSGYPQWMFFSLVNHGLQGFFAGFKGKSQWLGLIL
                     ATIAMVGGYALGSTLMNGWAAALPEILPNFMQNMVGMIVGFILSQSIKKIK"
     gene            complement(8609..8857)
                     /locus_tag="ACL73L_000277"
     CDS             complement(8609..8857)
                     /locus_tag="ACL73L_000277"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS0771328.1"
                     /translation="MASTSLQAPAKICFILIKLYSPYAFLSKYLGAILLSFFCYYAKE
                     IGCNNLTLSVWNDNEGALRFYQRQGMKPQETTMEMIID"
     gene            complement(8805..9887)
                     /locus_tag="ACL73L_000278"
     CDS             complement(8805..9887)
                     /locus_tag="ACL73L_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HGS0771329.1"
                     /translation="MSKLQQILTYLESEKLDVAVVSDPVTINYLTGFYSDPHERQMFL
                     FVLADQEPLLFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYHGLKTVFETAEFDNLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLDKTETDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQAALDFIKPGVTAHEVDRAARE
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFDLFTSTSKDLLYFD"
     gene            complement(10013..10795)
                     /gene="gatD"
                     /locus_tag="ACL73L_000279"
     CDS             complement(10013..10795)
                     /gene="gatD"
                     /locus_tag="ACL73L_000279"
                     /EC_number="6.3.5.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0140282 - carbon-nitrogen ligase activity
                     on lipid II [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II isoglutaminyl synthase subunit GatD"
                     /protein_id="HGS0771330.1"
                     /translation="MVYTSLSSKDGNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGAHVTVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILSQEIAEEYSDVKSKADFS"
     gene            complement(10795..12138)
                     /gene="murT"
                     /locus_tag="ACL73L_000280"
     CDS             complement(10795..12138)
                     /gene="murT"
                     /locus_tag="ACL73L_000280"
                     /EC_number="6.3.5.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_function: GO:0140282 - carbon-nitrogen ligase activity
                     on lipid II [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II isoglutaminyl synthase subunit MurT"
                     /protein_id="HGS0771331.1"
                     /translation="MNLKTTLGLLAGRSSHFVLSRLGRGSTLPGKVALQFDKDILQNL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRFGEIYTTYNMILEAIRKVPT
                     ATVLLNGDSPLFYKPTIPNPIEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGAYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDGQEYGIQIGGLYNIYNALA
                     AVAIARFLGADSQLIKQGFDKSRAVFGRQETFHIGDKECTLVLIKNPVGATQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLASRQIVRKEMN"
     gene            complement(12237..13553)
                     /locus_tag="ACL73L_000281"
     CDS             complement(12237..13553)
                     /locus_tag="ACL73L_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HGS0771332.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYTTVAGTGVDIFDAEAHFLS
                     NKVIEIQAGDEKQELTAETIVINTGAVSNVLPIPGLATSKNVFDSTGIQSLDKLPEKL
                     GVLGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYLEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAADLKLPYAVKEIPVAAMPRGHVNGDLRGTFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI"
     gene            13747..14973
                     /locus_tag="ACL73L_000282"
     CDS             13747..14973
                     /locus_tag="ACL73L_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HGS0771333.1"
                     /translation="MKSNRYIIAFAGVILHLMLGSTYAWSVYRNPIIEKTGWDQASVA
                     FAFSLAIFCLGLSAAFMGRLVEKFGPKVMGSLSAFLYAGGNILAGFAIDRQELWLLYL
                     AYGILGGLGLGAGYITPVSTIIKWFPDKRGLATGLAIMGFGFASLLTSPIAQHLIAGV
                     GLVETFYILGASYFIIMLLASQFIKRPNEQELAILSSSGKEKTASLTQGMAANQALKS
                     NRFYMLWIIFFINIACGLGLISAASPMAQEMAGLSTSHAAVMVGVLGIFNGFGRLLWA
                     SLSDYIGRPLTFSILLLVNLFFSLSLWLFTDSVLFVVAMSILMTCYGAGFSLIPAYLS
                     DIFGTKELAALHGYILTAWAMAGLAGPILLAETYKMAHSYTQTLFVFLILYSIALALS
                     YYLGRSIKKESQKPLT"
     gene            15172..16746
                     /locus_tag="ACL73L_000283"
     CDS             15172..16746
                     /locus_tag="ACL73L_000283"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGS0771334.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRTEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQDIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVAPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFADE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRGNGRRDEFKKGSRGNDRFDKEKRYRKDNKKPRNT
                     LSEKQTGFVIRNKGDK"
     gene            17010..17798
                     /gene="codY"
                     /locus_tag="ACL73L_000284"
     CDS             17010..17798
                     /gene="codY"
                     /locus_tag="ACL73L_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HGS0771335.1"
                     /translation="MAHLLEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDDYVQGANMIYETEANLPVEHDMSIF
                     PVESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEDLVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGKLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
     gene            17798..18373
                     /locus_tag="ACL73L_000285"
     CDS             17798..18373
                     /locus_tag="ACL73L_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HGS0771336.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDCFHPESKLFPPHNLIGTSGRNLYGDLGIFYQEHGSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFALGHFKNTLGAKLVDENLNELSE"
     gene            18455..18540
                     /locus_tag="ACL73L_000286"
     tRNA            18455..18540
                     /locus_tag="ACL73L_000286"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18489..18491,aa:Leu,seq:aag)
     gene            18823..19953
                     /locus_tag="ACL73L_000287"
     CDS             18823..19953
                     /locus_tag="ACL73L_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0771337.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKEDINKRVQEAAEILGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRVEQIGTPQEVYKNPVNKFVAGFIGSPAMNFINVKLVGSEIVSDGFRLKVPEGA
                     LKVLREKGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQV
                     GKDEFVAKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
     gene            complement(20100..20246)
                     /locus_tag="ACL73L_000288"
     CDS             complement(20100..20246)
                     /locus_tag="ACL73L_000288"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771338.1"
                     /translation="MEIKEQTRKLAVSCSKYIFEVADKTDEISNHTYGKATLTWFEEI
                     FEEY"
     gene            20454..21212
                     /locus_tag="ACL73L_000289"
     CDS             20454..21212
                     /locus_tag="ACL73L_000289"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGS0771339.1"
                     /translation="MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGFSKEKRILEVA
                     CNRGTTAIELAQRFGCKITAVDMDAQALEVAKKSAGTAGVAHLISFERANAMKLPYQD
                     ASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQA
                     IHVNVGPLTQDGWEQVMIESGYCDVKALTGEMTLMKLSGMIYDEGLLGTLKICVNACK
                     KENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR"
     gene            21300..21812
                     /locus_tag="ACL73L_000290"
     CDS             21300..21812
                     /locus_tag="ACL73L_000290"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HGS0771340.1"
                     /translation="MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYA
                     TDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIG
                     DYDYKVLMHY"
     gene            21994..22938
                     /gene="metA"
                     /locus_tag="ACL73L_000291"
     CDS             21994..22938
                     /gene="metA"
                     /locus_tag="ACL73L_000291"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HGS0771341.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAAHQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFPEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDDDVNQVPCLC
                     WSSSAALFFSNWVNHAVYQETPFDWRKIEDDASAYGYL"
     gene            22947..23624
                     /locus_tag="ACL73L_000292"
     CDS             22947..23624
                     /locus_tag="ACL73L_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HGS0771342.1"
                     /translation="MTYLDAFKSGTLVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEMSPSQIAERIGKEISDVNQSISNLTERGLLQYRTIELNGEIELLFDASLALERL
                     DDLFGAVHSSSDQLTSQNQLKDLVETFQQELGRLLTPFEIEDLTKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLTQVSADFRN
                     AMDLWKD"
     gene            23722..24480
                     /gene="tpiA"
                     /locus_tag="ACL73L_000293"
     CDS             23722..24480
                     /gene="tpiA"
                     /locus_tag="ACL73L_000293"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HGS0771343.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVASKLPSSDLVEAGIAAP
                     ALDLTTVLAVAKGSNLKVAAQNCYFENAGAFTGETSPQVLKEIGTDYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAEFVGAQVSAALAGLTAEQV
                     AASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVASYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            24518..26023
                     /gene="lytC"
                     /locus_tag="ACL73L_000294"
     CDS             24518..26023
                     /gene="lytC"
                     /locus_tag="ACL73L_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase LytC"
                     /protein_id="HGS0771344.1"
                     /translation="MSVTFFIGEQRLKTKIGLASICLLGLATSHVAANETEVAKTSQD
                     TTTASSSSEQNQSSNKTQTSAEVQTNAAAHWDGDYYVKDDGSKAQSEWIFDNYYKAWF
                     YINSDGRYSQNEWHGNYYLKSGGYMAQNEWIYDSNYKSWFYLKSDGAYAHQEWQLIGN
                     KWYYFKKWGYMAKSQWQGSYFLNGQGAMMQNEWLYDPAYSAYFYLKSDGTYANQEWQK
                     VGGKWYYFKKWGYMARNEWQGNYYLTGSGAMATDEVIMDGARYIFAASGELKEKKDFN
                     VGWVHRDGKRYFFNNREEQVGTEHAKKIIDISEHNGRINDWKKVIDENEVDGVIVRLG
                     YSGKEDKELAHNIKELNRLGIPYGVYLYTYAENETDAENDAKQTIELIKKYNMNLSYP
                     IYYDVENWEYVNKSKRAPSDTDTWVKIINKYMDTMKQAGYQNVYVYSYRSLLQTRLKH
                     PDILKHVNWVAAYTNALEWENPYYSGEKGWQYTSSEYMKGIQGRVDVSVWY"
     gene            26520..27038
                     /locus_tag="ACL73L_000295"
     CDS             26520..27038
                     /locus_tag="ACL73L_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HGS0771345.1"
                     /translation="MVELKKEAAKDVATLTKAAPVALAKTKEVLNQAVADLYVAHVAL
                     HQVHWYMHGRGFLVWHPKMDEYMEALDGQLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEAGEYRNVEESLERVLAIYRYLITLFQKALDVTDEEGDDVTNDIFVGAKAELEKTVW
                     MLAAELGQAPGL"
     gene            27168..27674
                     /locus_tag="ACL73L_000296"
     CDS             27168..27674
                     /locus_tag="ACL73L_000296"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HGS0771346.1"
                     /translation="MTKKIVAIWAQDEEGVIGKDNRLPWHLPAELQHFKETTLNHAIL
                     MGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATFQDVQSVLDWYQAQEKNLYIIGG
                     KQIFQAFEAYLDEVIVTHIHARVEGDTYFPEELDLSLFETVSSKFYAKDEKNPYDFTI
                     QYRKRKEV"
     gene            27674..27844
                     /locus_tag="ACL73L_000297"
     CDS             27674..27844
                     /locus_tag="ACL73L_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771347.1"
                     /translation="MERSLFGLFTAFLCFICFLAGAQAFRKKRYGLSILLWLNAFTNL
                     VNSIHAFYMTLF"
     gene            27876..29108
                     /gene="clpX"
                     /locus_tag="ACL73L_000298"
     CDS             27876..29108
                     /gene="clpX"
                     /locus_tag="ACL73L_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HGS0771348.1"
                     /translation="MSTNRKNDMMVYCSFCGKNQEEVQKIIAGNNAFICNECVELAQE
                     IIREELVEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKSENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNNKAI
                     DENSSYMQEIIAEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELEFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKETVDGTDKPILETA"
     gene            29117..29704
                     /gene="yihA"
                     /locus_tag="ACL73L_000299"
     CDS             29117..29704
                     /gene="yihA"
                     /locus_tag="ACL73L_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HGS0771349.1"
                     /translation="MELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYL
                     TTRENLRAVVSLVDLRHDPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKL"
     gene            29715..30095
                     /locus_tag="ACL73L_000300"
     CDS             29715..30095
                     /locus_tag="ACL73L_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HGS0771350.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            30147..31037
                     /gene="rapZ"
                     /locus_tag="ACL73L_000301"
     CDS             30147..31037
                     /gene="rapZ"
                     /locus_tag="ACL73L_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HGS0771351.1"
                     /translation="MTKKQLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAADGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVFDVRFLPNPYYLPELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKRLVQDLSKN
                     WSVNEGHRDKDRRKETVNRS"
     gene            31034..32011
                     /locus_tag="ACL73L_000302"
     CDS             31034..32011
                     /locus_tag="ACL73L_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HGS0771352.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDFRNVLVAMSDMPKFYEKVFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIYPSSDHPLTLHAVFQDGTEVAGESHIVDHRGIIDNV
                     YVTNALNDDTPLASRRVVQTILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSDHVEVLHRHLGRPFIDTVLVNIEKVPQEYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            32008..32919
                     /gene="whiA"
                     /locus_tag="ACL73L_000303"
     CDS             32008..32919
                     /gene="whiA"
                     /locus_tag="ACL73L_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HGS0771353.1"
                     /translation="MSFTVAVKEEILGQHHLSWHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHFYEIKSEIRHHQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETGIDEAILSDEEAGRAYLCGAFLANGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKTINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIADEL"
     gene            complement(32953..33921)
                     /locus_tag="ACL73L_000304"
     CDS             complement(32953..33921)
                     /locus_tag="ACL73L_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HGS0771354.1"
                     /translation="MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEEFAITTS
                     KGSHLTKTVIIAMGGGAFKPRPLELEGVEGYENIHYHVSNIQQYAGKKVTILGGGDSA
                     VDWTLAFEKIAPTTLVHRRDNFRALEHSVQALQESSVTIKTPFAPSQLLGNGKTLDKL
                     EITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLNRHKIIVNSKQESSQAGIYA
                     IGDCCYYDGKIDLIATGLGEAPTAVNNAINYIYPEQKVQPKHSTSL"
     gene            34057..34914
                     /gene="cdaA"
                     /locus_tag="ACL73L_000305"
     CDS             34057..34914
                     /gene="cdaA"
                     /locus_tag="ACL73L_000305"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HGS0771355.1"
                     /translation="MNFQQLSNLQYWTSLFASPWTIAINLIDILIVAYILYHFTKAIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYMSTGIPLD
                     AKISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERL
                     LGGWKHEKK"
     gene            34901..35680
                     /locus_tag="ACL73L_000306"
     CDS             34901..35680
                     /locus_tag="ACL73L_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HGS0771356.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSTSARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVSVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEDLPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEVSITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            35704..37056
                     /gene="glmM"
                     /locus_tag="ACL73L_000307"
     CDS             35704..37056
                     /gene="glmM"
                     /locus_tag="ACL73L_000307"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HGS0771357.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEAPKV
                     FVGRDTRISGEMLESALVAGLLSVGIHVYKLGVLATPAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPEALQEVVKESGSAIGLAFDGDSDRLIAVDENGDIVDGDKIMYIIGKYLSEKGQLA
                     QNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAVQLTKIMKETGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KAIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTTEEVDYYVDTITDVVRAEIGID
                     "
     gene            37130..37504
                     /locus_tag="ACL73L_000308"
     CDS             37130..37504
                     /locus_tag="ACL73L_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HGS0771358.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWENENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPSELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            37506..38354
                     /locus_tag="ACL73L_000309"
     CDS             37506..38354
                     /locus_tag="ACL73L_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HGS0771359.1"
                     /translation="MKLAVFTDSSAYLSAETLQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASELPKTSQPSIAKLDEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVESVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASGQYRVIVIHGNAPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGAGSIAL
                     GYIPVI"
     gene            38620..39387
                     /gene="dapB"
                     /locus_tag="ACL73L_000310"
     CDS             38620..39387
                     /gene="dapB"
                     /locus_tag="ACL73L_000310"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HGS0771360.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLTDSDLDLVAVLDPFESESEWQ
                     GIPVFKDKADLAGFEADVWVDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRAQDLGGLIAPNFALGAVLLMQFATQAAKYFPNVEIIELHHDKKKDAPSGTAIKT
                     AELMAEVRESIQQGAADEEELIAGARGADFDGMRIHSVRLPGLVAHQEVIFGNQGEGL
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLEHLL"
     gene            39384..40583
                     /locus_tag="ACL73L_000311"
     CDS             39384..40583
                     /locus_tag="ACL73L_000311"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HGS0771361.1"
                     /translation="MRLTQMPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHSPIH
                     DVDIATSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSA
                     VSFVRSLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDA
                     LRIMRGFRFQASLGFALEPETFKAMKTLTPLLEKISVERTFVEFDKLLLAPFWRRGLA
                     SMIESQAYDYLPDMASSQNKLNRLFDLETDFTFESSEQAWAALLWALEIENAQSFLKS
                     WKTSRQFAKQVQDLLIILALRENGELSKRDCYRFDIDLLLQAENLRQARGKEVNPQAI
                     TEKYQSLTIHDKKDIQINGDILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIH
                     AYLREKK"
     gene            40580..42451
                     /locus_tag="ACL73L_000312"
     CDS             40580..42451
                     /locus_tag="ACL73L_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HGS0771362.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKNDYQIGYLTQDPDFDDRKTVLDTVLSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLSKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFLKNSKKTVLFITHDRYFLDAL
                     STRIFELDRAGLTEYRGNYQDYVRLKAEQDERDAALLHKKEQLYKQELAWMRRQPQAR
                     ATKQQARINRFHDLKKEVSGSSAETDLTMNFETSRIGKKVIEFQAVSFAYENKPILQD
                     FNLLVQAKDRIGIVGDNGVGKSTLLNLIAGSLEPTKGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDGKIRPFFGHYTDYLDEKAFETDMANQVQKAEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIEALEKRIAAIEEEMQANGSDFGKLATLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(42594..43778)
                     /gene="mntE"
                     /locus_tag="ACL73L_000313"
     CDS             complement(42594..43778)
                     /gene="mntE"
                     /locus_tag="ACL73L_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HGS0771363.1"
                     /translation="MKQSISNLKLAERGAIISISTYLILSAAKLAAGHLLHSSSLVAD
                     GFNNVSDIIGNVALLIGIRMARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDVLRD
                     TIQKILSREETVIDPLGATLGIISAAIMFVVYLYNTRLSKKSNSKALKAAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYDIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLENRF
                     GVFDTDVHIEPAPIPEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKEAYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
     gene            44082..46778
                     /locus_tag="ACL73L_000314"
     CDS             44082..46778
                     /locus_tag="ACL73L_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HGS0771364.1"
                     /translation="MSKEQKRQAFYTQSPEEVLQAVDATEQGLSSSEAEKRLAEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVLRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LIEANSLKIEEAALTGESVPVEKDLSVELATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILVIALVTFVVGVFIQGKN
                     PLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVVLANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFDSDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKAGDITPIDEKVTNLIHTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEGHVLTGAELNELSDEEFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEVSKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMAGSFSMIIIVEIVKFIQRKLGFDKNAI"
     gene            46791..47582
                     /gene="yghU"
                     /locus_tag="ACL73L_000315"
     CDS             46791..47582
                     /gene="yghU"
                     /locus_tag="ACL73L_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HGS0771365.1"
                     /translation="MSAYQLPTVWQDEASNQGAFTGLNRPTAGARFEQNLPKGEQAFQ
                     LYSLGTPNGVKVTILLEELLEAGFKEAAYDLYKIAIMDGDQFGSDFVKLNPNSKIPAL
                     LDQSGTENVRVFESAHILLYLAEKFGAFLPSNPVEKVEVLNWLFWQAGAAPFLGGGFG
                     HFFNYAPEKLEYPINRFTMEVKRQLDLLDKELAQKPYIAGNDYTIADIAIWSWYGQLV
                     QGNLYQGSAKFLDASSYQNLVKWAEKIANRPAVKRGLEVTYTEIK"
     gene            47698..48108
                     /locus_tag="ACL73L_000316"
     CDS             47698..48108
                     /locus_tag="ACL73L_000316"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HGS0771366.1"
                     /translation="MEKKIVKDILFLSQVSQPASQEDLYLARDLQDTLLANRDTCVGL
                     AANMIGVQKRVIIFNLGLVPVVMFNPVLLSFEGSYEAEEGCLSLVGVRSTKRYETIRV
                     AYRDSKWQEQTITLTGFPAQICQHELDHLEGRII"
     gene            48120..49715
                     /locus_tag="ACL73L_000317"
     CDS             48120..49715
                     /locus_tag="ACL73L_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771367.1"
                     /translation="MKRIVFELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNLEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPKSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADLVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVNDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRIYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTN
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYALVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESIKGVVADLKSAVIKGDTVYFFKVDGKIYKVKASVSDDLPY
                     LENGKTFEGQVGKDNYLKTFKVQ"
BASE COUNT        14465 a         9331 c        11364 g        14607 t
ORIGIN      
        1 cgacgttgac gtggtttgaa ttggattttc gaagagtata agcattttac tgaaatttta
       61 tcaggtagtt taggatggca aatgacattc cctgtcaaaa atggtaaaat aagaaaaaat
      121 aatctgagaa tcgaggaaaa aagatgcaag aaaagatttt ggtaactggt ggtgccggtt
      181 ttattggaac ccacactgtt attgaattga tccaagcagg ccatcaagtt gttgtggtgg
      241 ataatcttgt caatagcaat cgtaagagtt tagaagttgt tgaaagaatc acaggagttg
      301 agattccttt ctatgaggca gatattcgtg acacagctac cctcagagat attttcaagc
      361 aggaagaacc gactggtgtc attcactttg ctggtttgaa ggctgttggc gaatcaacac
      421 gtatccctct tgcctactat gacaacaata tcgctggaac tgtcagcctt ttgaaagcca
      481 tggaagaaaa caactgtaaa aacatcatct tcagttcttc tgcgacagtt tacggggatc
      541 cgcacacagt gcccatcttg gaagatttcc cactttcagt gaccaaccca tacggtcgta
      601 ctaagctcat gctagaggaa attttgactg atatttacaa agcagactca gaatggaatg
      661 ttgtcttgct tcgttacttt aacccaatcg gagcccatga gagtggtgat ttgggagaaa
      721 atccaaacgg tattccaaac aatctcttgc catatgtgac tcaagtagcc gttggaaaat
      781 tagagcaagt gcaagtgttt ggagacgatt acgatacgga agatggaaca ggtgttcgtg
      841 actatatcca cgttgtcgat ttggctaagg gtcacgttgc agctttgaaa aaaatccaaa
      901 aaggttcagg actaaacgtt tataaccttg gaactggtaa aggttactca gttcttgaaa
      961 ttatccaaaa catggaaaaa gcggtgggac gtcctattcc ttaccgcatc gtagaacgtc
     1021 gcccaggtga tatcgctgcc tgctactcag acccagcaaa agctaaagca gaactcggtt
     1081 gggaagcaga actcgacatc acccaaatgt gtgaagacgc atggcgttgg caaagcaagc
     1141 atccaaatgg atttgaagac taagatgatg atttcaatca tcgtcccttg tttaaacgaa
     1201 gaggaagtac ttcctctttt ttatcaggct ctggaagctt tacttccaga tttggaaaca
     1261 gaaatcgagt atgtctttgt cgatgatgga tcaagtgatg ggaccttgga actcttaaag
     1321 gcctatcggg agcaaaatcc ggcagtccat tatatttctt tctctcgaaa ttttggcaaa
     1381 gaagcagccc tttatgcagg cttgcaatat gcgacaggag atttggtggt ggtgatggat
     1441 gcagacctcc aagatcctcc tagtatgttg tttgagatga aaaatgtact agacaaaaat
     1501 gtagacttgg actgcgttgg gacacggaga actagtcggg agggagaacc cttctttcgc
     1561 agtttctgtg ctgttctctt ttatcgcctc atgcaaaaaa tcagcccagt agctctgccg
     1621 tcgggtgtcc gtgattttcg tatgatgaga aggtctgtag tcgatgccat tttaagcttg
     1681 accgagtcca atcgtttttc aaaggggctc ttcgcctggg tgggtttcaa aacctactat
     1741 ttggactatc caaatgtcga aaggcaggct ggcaagacca gttggagttt taggcaactc
     1801 tttttttact ccattgaagg gattgttaat ttttcagatt tccctttgac tatagccttt
     1861 gtagctggtc tcctatcttg ttttctttct ctgctgatga ccttttttgt tgtggttcgg
     1921 accctcattt tgggcaatcc gacatctggt tggacctctc tgatggctgt tattctcttt
     1981 cttggaggca ttcaactctt gaccattggg attctcggta agtatatcag taagatttat
     2041 ttagagacta aaaaaagacc actttatctt atcaaagaaa aaagtgacct tcctgatttt
     2101 acagaaaaaa taaagtgaaa agactataat tttacatgga aatgtgctaa actagaggga
     2161 gtggattatc gccattgatt taggcttcct ggtttccaat cagggcgcga gttgggcaat
     2221 ttttaacagc ctctaagaca tcctgactag gagaaatttc tttttccagt tggtcagggt
     2281 catcgtaaaa acgcacgatt ccattatcgt ggtaatcaaa taaatcagaa taagtttggc
     2341 aaagtccaca ggcgatacat cgttcaggta taagtgtgat tttcatgttc atatttatat
     2401 tgtaataaga aaagtaaaaa aaaacaagga gtaaggtatg gcaaaagaac cgtggcaaga
     2461 agatatttat gatcaagaag aatcaagagc agggcatcgg catcgaaacc acggaggggc
     2521 tgataggatg gctaatcgta ttttgacgat cctagctagt attttctttg taattgtggt
     2581 ggtgatggtc atcgttctca tctatctatc atcggggggg agtaatcgca cagcagcctt
     2641 aaaagacttt catgattctg atgcaagtgt agtacaaatc tcatcttcaa gcagttctca
     2701 gcctgagcag agttcagagc cagaatctac ttctagtagt tcagaagaag ctgctaatcc
     2761 tgaaggaacg attaaagttc tcgcaggaga aggggaagca gctattgccg ctcgtgcagg
     2821 aatctccatt gctcagttag aggccttgaa tcctgggcac atggctacag gatcttggtt
     2881 tgctaatcca ggtgatgtta taaaaataaa ataggagccg atatgaaaac aattcaaatt
     2941 gctattgatg gtcctgcttc cagcggtaag agtacggtcg caaagattat tgctaaggat
     3001 tttgggttca cctaccttga tacaggagct atgtatcgtg cagcgaccta tatggctctt
     3061 aagaaccaat taggagttga agaagtcgaa gcccttctag ccttgttgga ccagcatcca
     3121 atcagctttg gacgttcaga aactggagac cagcttgttt ttgtaggaga tgtggatatt
     3181 acccatccta tccgtgaaaa tgaagtgacc aatcatgttt ctgctattgc agcaattcct
     3241 gaagtgcgtg agaaactggt ttctctccaa caagaaattg cccagcaagg cgggattgtc
     3301 atggatggtc gcgatattgg aactgttgta ttgccacaag cagaattgaa aattttccta
     3361 gtagcttctg ttgatgagag agcagagcgt cgttacaagg aaaatattgc caagggaatt
     3421 gaaacagacc ttgaaaccct aaaaaaggaa attgctgcgc gtgactacaa ggatagtcat
     3481 cgtgagactt ctcctctcaa acaagcagag gatgctgtct accttgatac aactggtttg
     3541 aacattcaag aagtagttga aaaaatcaaa gcagaagctg aaaaaagaat gtaaatgtag
     3601 aaaagccgat agattgatat cggttttttt ctaaatttgt caaaatgctg attttagggt
     3661 ataatagttg ctgttcgaga aattttgatt ttcaaagaat agtgaatgat gataaggaga
     3721 aaccatgaac aatttaccaa attgtccaaa atgtaactca gagtatgtct acgaagacgg
     3781 tgctctactg gtttgcccag agtgtgctca tgagtggaat cctgctgaag ttgcagaagt
     3841 agaagaaggt cttgtcgcta tcgatgccaa cggaaataaa ttgactgatg gtgatacagt
     3901 aactctcatc aaggacttga aagtaaaagg tgcgccaaaa gatttgaaac aagggacgcg
     3961 cgtgaaaaat atccgcatcg tagaaggcga tcacaatatc gactgtaaga ttgatggttt
     4021 tggtgccatg aaacttaaat cagagtttgt gaggaagatt taagcatgtc aaaacactat
     4081 aaacttgtat tttatagccg tatcttcttg tttctagcgg cttttacggg agtttatctt
     4141 gaaattacta ggcatggtgg ttttgggatg cttctctatt acacggttct gtccaacctc
     4201 ttggtaacta tttttaccct ttatcttcta aaggttatga gccgtgtagg tgaaaattgg
     4261 caaaggccaa gtctcttgcg cttaaaaggt ggggtcacca tgagtatcat gattacctgt
     4321 gtgatttacc atttcctctt ggcgcccatt gcgactaatt tctataccct agaaaatttc
     4381 ctttgccact atatcgttcc catctggttt ttagcggata ccctcttttt tgacaaacag
     4441 ggtcaataca agatttggga tccagcagtg tggaccattt taccctttct gtatatgatg
     4501 tttgctcttt ttaatggctt ggttctaaaa ctcaatattc caaatgccaa ggataatcct
     4561 ttcccttact tctttttgaa tgtgaacaag ggttggaatg ttgtgtttaa gtggtgtctg
     4621 attatctttg ttgcctatat ggtagcagga tttattttct actttatcaa gcaaatcaag
     4681 agaaagtcat cctaatactc ttcgaaaatc tcttcaaacc acgtcagttt ccatctgcaa
     4741 cctcaaaaca ctgttttgaa caacctgcgg ctagcttcct agtttgctct ttgattttca
     4801 ttgagtataa gatacaaggg attcattttc gaatttaaga aggagtctac aaaccaatga
     4861 ggcttcttct tttcttgctt atttgataac tatcaaaaaa gtagaagatt aagagaaata
     4921 tagaaagaga tttcatgaaa caatttttag aacgggccag cattttggct ctctccctcg
     4981 ttttgattac ctccttttcc atttcgagtg ccctaccagc catgtttgac tattatcagg
     5041 gttattctaa ggaacaaatt gagctcttgg tgagcttgcc ttcctttgga atcatgatga
     5101 tgttactgct aaatggtttc ttagaaaaaa tatttcctga gcgcttacag attagtttgg
     5161 gcttgctgat tttatcattg agcggtacag ctcccttctg gtaccaagcc tatccctttg
     5221 tctttggaac acggcttctc tttggtttgg gtcttgggat gatcaatgcc aaggccattt
     5281 ctattatcag tgaacgctac caaggaaaaa ggcgaattca gatgttaggg ctacgcgctt
     5341 ctgcagaggt cgttggagct tctctcatta ccttggccgt cggtcagttg ttggcctttg
     5401 gttggacagc tatctttcta gcctatagtg ctggattttt ggtgctgccc ctttatctgc
     5461 tctttgtccc ttatggaaaa tcaaagaaag aagtcaagaa aagagcgaag gaagcaagtc
     5521 gtttaactcg agaaatgaaa ggcttgattt ttaccttagc tatcgaagcg gcagttgtag
     5581 tttgtaccaa tacagctatt accatccgta ttccaagttt gatggtggaa agaggattgg
     5641 gggatgccca gttatctagt tttgttctta gtatcatgca gttgatcggg attgtggctg
     5701 gggtgagttt ttctttcttg atttctatct ttaaagagaa actgctcctc tggtctggta
     5761 ttacctttgg cttggggcaa attgtgattg ccttgtcttc atccttgtgg gtggtagtag
     5821 caggaagtgt tctggctgga tttgcctata gtgtagtctt gacgacggtc tttcaacttg
     5881 tctctgaacg aattccagct aaactcctca atcaagcaac ttcatttgct gtattaggct
     5941 gtagtttcgg agcctttacg accccatttg ttctaggtgc aattggctta ctaactcaca
     6001 atgggatgtt ggtctttagt atcttaggag gttggttgat tgtaatctct atctttgtca
     6061 tgtacctact tcagaagaga gcttaggatt gattcctaag tttttctttt gatactcaat
     6121 gaaaatcaaa gagcatagtt gattgagttt ggaatagtac gctgtagttg ctaaaatatt
     6181 tctagaaatt aatttgactt ttctaataga gttattcata tcttatttca atctactata
     6241 gaaagctagc cgcaggttgc tcaaaatagt gttttgaggt tgcagatgga aactgacgtg
     6301 gtttgaagag attttcgaag agtatgagat ttttataccc atacaatatt tgttttaggt
     6361 ccttgtttac agactaattt cttgataaaa tagaggttat gacgagatat aaagcaacta
     6421 tttcctatga tggttatgcc tttgctggct ttcagcgcca gtctcatgcg cgtagcgttc
     6481 aggaagaaat tgaaaaaacc ttgaccagat taaataaagg gcaaaccatt actgttcacg
     6541 gtgctggtag gacagatagt ggggttcatg ccctgggaca ggtcattcat tttgacctgc
     6601 cttatcagat ggatgaggag aaactccgtt ttgccttgga tacccagtct cctgaagata
     6661 ttgatgtgat ttcgattgag cttgtggcag atgattttca ttgccgttat gccaaacata
     6721 gcaagaccta tgagtttatt gtggatagag gacgtcccaa aaatcctatg cgccgtcact
     6781 atgccaccca ctttccctac ccactcgatg tggaacgaat gcagattgca atcaaaaagc
     6841 tagagggaac ccatgatttt accggtttta cagcctctgg gactagtgta gaggataagg
     6901 ttcgcaccat cacagaagct agtttaatag tcgatgagac aggacaattt ttgaccttta
     6961 ccttttcagg aaatggtttc ttgtataaac agattcgcaa tatggtgggg actcttctca
     7021 aaatcggtaa caaccgcatg ccagtagagc agattgatct catcttggag aagaaggaca
     7081 ggcaacttgc aggtcccact gcagcaccaa atggtttgta tttaaaggag attcgttatg
     7141 aagaataatc gtattttagc actttctgga aatgatattt ttagtggtgg tggactgtca
     7201 gctgatttgg ctacctatac cttgaacggc ttgcatgggt ttgtagcagt gacttgtttg
     7261 acagccttga cagaaaaagg atttgaagtc tttccaactg atgataccat ttttcaacat
     7321 gaattagata gcttgcgtga tgtggaattt gggggaatta agattggtct tctccctact
     7381 gtcagtgtgg ctgagaaggc cttggacttt atcaaacaac gcccaggagt acctgtggtg
     7441 ttggatcctg tcttggtctg caaggaaacg catgatgtag ctgtcagtga gctctgccaa
     7501 gagttgattc gctttttccc ttatgtcagt gtgattacgc ctaatctccc agaagcagaa
     7561 ttattatccg gtcaggaaat taaaaccttg gaagacatga aaactgcagc gcagaaattg
     7621 catgatttag gagcgccagc agtcattatc aagggaggca atcgtcttag tcaggacaag
     7681 gctgtggatg tcttttatga tggacagacc tttactatcc tagaaaatcc agttatccaa
     7741 ggccaaaatg ctggtgcagg ttgtaccttt gcctctagca ttgccagtca cttgattaaa
     7801 ggtgataaac ttttgccagc agtagaaagc tctaaggctt tcgtttatcg tgctattgca
     7861 caagcagatc agtatggagt aagacaatat gaagcaaaca aaaacaacta aaatcgccct
     7921 tgtatcccta ttaaccgccc tttctgtggt tctaggttat ttcttaaaaa tcccaacacc
     7981 tacaggaatt ctaactcttt tagatgctgg tgtcttcttt gcggcctttt actttggtag
     8041 tcgtgaagga gcggtagtcg gaggactagc aagtttcttg attgacctct tatcaggcta
     8101 ccctcagtgg atgttcttta gcttggtcaa ccatggcttg cagggatttt tcgcaggatt
     8161 taaaggaaaa agtcagtggt taggccttat tttagcaact attgccatgg taggaggcta
     8221 cgccttgggt tctactttga tgaatggctg ggcagcagcc ctcccagaaa ttctaccaaa
     8281 tttcatgcaa aatatggtag ggatgattgt aggatttatt cttagtcaaa gtatcaagaa
     8341 gattaagtaa tactcttcga aaatctcttc aaaccacgtc agtttccatc tgcaacctca
     8401 aaacactgtt ttgagcagcc tgcggctagc tttctagtct gattttcatt gagcataaag
     8461 aggttgagta aaaagtcttt caaataagaa aaacgcatag tatcaggtgt tgaatgaaca
     8521 cctgatacta tgcgttttat tgtgggaaga ttttcctcat tttctagtga aattgaactt
     8581 tttctaacat cttttgatag atgacttctt aatcaattat catttccatt gttgtttctt
     8641 ggggtttcat cccttgtctt tgataaaagc ggagagcacc ctcgttatca ttccagacac
     8701 ttagggttag attattgcag ccaatttctt tagcataata gcagaaaaat gacaataaaa
     8761 tagcccctag atattttgaa aggaaagcat aggggctata tagtttaatc aaaataaagc
     8821 aaatctttgc tggtgcttgt aaagaggtcg aagccatcct tggtaacaac accgcagtct
     8881 tcaatacgaa caccgacttt accagggata tagatacctg gttcaacaga gaagcacatg
     8941 ccttcttcga tgaccatgtc gtttccttcc atgatagatg ggaattcatg gacatccata
     9001 ccgataccat gcccgagacg gtggttgaag tactcaccat aaccagcttt ttcgatgacc
     9061 tcacgggcag cgcggtccac ttcatgagca gtcacacctg gcttgataaa gtcaagagca
     9121 gcttgttggg cttcaagagt caagttgtaa atatctttct tgaattggtc tggtttgccg
     9181 acagcgactg tacgagtcat atctgacgca tagccattga ccagaacacc caggtcaaag
     9241 aggagaagag catcattttc aaccttatta gctgctggaa tgccgtgtgg attcgcagca
     9301 ttatcaccag tcaagaccat ggtatcaaag ctcatttcgt aaccttcacg tttcatggca
     9361 aagtcaattt gtgcgatgat atctgtctca gtcttatcaa gagaaatatt gtcaaaacca
     9421 acatgaacag ccttgtcagc ataaagacct gcaaccatca ttttttgcac ttcatcagct
     9481 gatttgatga ggcgcatgcg ttggatacga ggagtgaggt tgtcaaactc agcagtctca
     9541 aaaactgttt tcaaaccatg gtatttggtc aagatgagat tgtcaaactc aacagcgaca
     9601 cgtttgaagt caagttgtgg aagagcatgt ttgatttttt gccatggatt ttcagaatcg
     9661 acatagccca ctactgggaa ggaaacggtg ctacttgcac gttctacttc aagagctggg
     9721 acaaagagga gaggttcctg atctgctagg acaaagagga acatttggcg ttcatgggga
     9781 tcactgtaaa aaccagtgag gtaattgatt gtgacggggt cagatacgac agcgacgtct
     9841 agtttttctg attcaagata tgttaggatt tgttgtaatt tagacatatg ctaccttctt
     9901 tctatccctc tattttggca aaaaatgaga gaaaatgcaa gggagaaggg taaaagaaaa
     9961 gacaaaaaac tccgacaaga taacggagtt ctttgatatc attttccttt gtttaagaaa
    10021 agtcagcctt gcttttgacg tcactgtact cttcagcgat ttcttggctg aggatatcct
    10081 cataggcagg gagttggata tcctgaccat atttcttctt gagggcagta gtaactaggc
    10141 gataagccag attggcatta cgagagagga taggcccgtg gaagtaggaa ccaaagacat
    10201 tcttataatg aaccccttca ccgacctttt cttcgttgtt tccatttcca tagacaacct
    10261 gtcccagcgg tttttggtca tcagagagga aggtacggcc ttggtgattt tcaaatccat
    10321 agtaggtttc atcgaaatct tcattgtgaa tcttgatgtc accgataaaa cggttattgg
    10381 tctggttgag cgtgtagtgt cccatgaccc ctagcccttc gatacgtttt cctgaagctt
    10441 caacataata ttgacccaat agttggaaac caccgcagat agccagaact acaccgtcgt
    10501 tttggatgta gttgtcaatg ctctcttttt tagcaggtag gtcgtctgca atgatacttt
    10561 gttcaaagtc ttgaccacca ccgaaaaagg cgatgtcgta gtgattttca tcaaagtcat
    10621 catggagaga aacgatgtca acggtcacat gggctcccag tttttcagcc acatacttga
    10681 gcatgaggat gtttccattg tccccgtagg tattcatgag atttccgtag aggtgggcaa
    10741 tgttgagctg ataggggtaa ttgccatctt ttgaggaaag tgaagtataa accattagtt
    10801 catctccttt ctaacaatct gacgactagc cagcagttca cgaaattcca gcatggcagt
    10861 ataagttgcc agaatatagg catgcttgca gtcttgattc tcaatggtct tgagaacttg
    10921 ctccagatta ctcgtttcag tgattttctc agctggatag ccagtcactc ggaggcgacg
    10981 agcgatttca gaatgacgaa caccgccagc gttgatttca ggaatgtcca tgtcagtgat
    11041 ttgttcaaag tctgcatccc agatccagct agtgtcaatt ccatctgcat agttggcatt
    11101 aaggaggaca gataggctaa atggataagg tgctagtttg atcatttcga tagcttgggt
    11161 tgcaccgact ggatttttaa tcaagacaag ggtacattcc ttgtcaccga tatgaaaggt
    11221 ttcttggcgt ccaaagacag cacggctctt gtcaaatccc tgtttgatga gttgcgaatc
    11281 ggcacctagg aaacgggcga tggccacagc agctagggcg ttatagatat tatagagccc
    11341 gccgatttgg ataccgtatt cttggccgtc tatgacaaag cgagagcgat tgttggtcaa
    11401 ctcaaccagt tttgtcaaac gatagtcgag atcaggacgt ttgcatccac aaccttcaca
    11461 gatataggca cccaagtttg cataggtatt atgctcatat ttgaggatgc cttggcagtc
    11521 aggacagaga atcccttcgg tattgtagtg agccagttgg gctggtcctt tttccaagtc
    11581 aaaaccaaaa tactctatag ggtttggaat agttggcttg tagaaaagtg gactgtctcc
    11641 gttaaggaga acagtagcag ttggaacttt ccgaatggct tccaatatca tgttataggt
    11701 agtatagatt tcaccgaaac ggtccatctg gtcacggaag atattagtaa tgacaaaaag
    11761 actaggctgg atatagtcac agatacgaga tagactggct tcgtcaattt cgaggacggc
    11821 aatatttttc ccagtttttg aagatttggc tgttaggaag gttgttgcaa tccctgtaat
    11881 catgttggca ccgcttgggt tggttagaac ttgaccataa acctctttta aaatgccgac
    11941 agtgagggca gttgtcaggg tttttccatt tgttccagtg acaacgacaa tctcgtagtt
    12001 cttagctagg ttttgtaaaa tatctttatc aaattgaagg gcgactttac ctgggagcgt
    12061 acttccacgt ccaagacggc ttaaaacgaa gtgggaagaa cgcccagcaa gaaggcccaa
    12121 agtagttttt aagttcatgt ttctattata tcacaaaaga gggaaaagac agatttggtg
    12181 tttcgtagaa gtaaaaacag cccaaagagg gctgtcagtt aagatgagat ttcaacttaa
    12241 atcgcaaaca agtcattcaa gttctcagcc aaggttgggt gagtgaagat ttgttttgtg
    12301 aagtaagtgt aaggaatctt gttgtccata gcaacagtaa tgatgttgat gatttcttga
    12361 gaaccttctg agaagatgct tgcaccaaga atttcttttg tttcagtatt aacaacagct
    12421 ttgaaagttc cgcgaaggtc tccatttacg tgaccacgag gcatggctgc aacagggatt
    12481 tctttcactg cgtatggaag tttcaaatca gctgcttggc tttcagtcaa accaacttgt
    12541 gaaagtgcag gtgtgatgaa catagtattt ggcacattga gacggtcctc aagtgtgtag
    12601 ctgccatctc cagcaaggta gctgtaaaca acacggaagt catcaagtga aatgtaagta
    12661 aattgaagac caccgttgac atctccaact gcaaagacac caggaacgtt tgtttgacag
    12721 tgtttatcta ctttaatagc gccacgttca gttagttcaa tatctgtatt ttcaagttga
    12781 agtggttcta catttggttt gcgtccagtt gcgtagagaa gggcgtcgaa acggtaagtt
    12841 tcgtcttcag ttacgacaag cacttggtca ccgtcgtttt taatttcagt agtatggata
    12901 ttttgaagca attcaatacc gtcttcttcc aggtattgtt tagcaagagc tgcgatggaa
    12961 ggttctgcac gaggtaggaa tgtatccaag gcatctagga ctgtaacctt gcttcctagt
    13021 ttattgtaaa ggccagcaaa ttcaagaccg atatttccgc caccaaggac tccaagtttt
    13081 tcaggcaatt tatccaagct ttggatacct gttgagtcaa agacgttttt gcttgtagca
    13141 agtccaggga ttggcaagac gtttgaaaca gcaccagtgt tgatgacgat agtttcagca
    13201 gtcaattctt gtttttcatc accagcttgg atttcgatga ctttatttga aaggaagtga
    13261 gcttcagcat caaagatatc gacgccagta ccagcaacag tcgtatagtt tttaccgttg
    13321 aggcgaccag tgatcgtgtt tttggtagca atgacttctt caaaagacaa gtctttctca
    13381 gcagcaacca gcaaagtttt agttgggata caaccgatgt tgatacaagt tccaccgtac
    13441 atagctttgc tacgttcaac gagggcaact tttttgccag ctgaagccaa tttaccagct
    13501 agtgttttac cagctttacc aaatccgata acgattaaat cataagttaa catttagaat
    13561 tcctcctatt cgaattacat tctagcacaa gaaaaaaact tttgcacaat tctgctacga
    13621 aaaagacaga aaaatatgaa aacgctttcc aggcaaaaaa ctagtaattt tttgacaagc
    13681 ttgataaact aaaatcctat ctatttttat ataagataga ataaatgttc gaaaatagga
    13741 ggttttatga aatcgaatcg ttatattatt gcctttgctg gggtcatttt acacttaatg
    13801 ctgggttcga cttatgcctg gagtgtttat cgtaacccta tcattgaaaa aacgggatgg
    13861 gatcaggctt ctgttgcctt cgcctttagt ctagcaatct tttgtttggg cttatcggct
    13921 gcatttatgg ggcgtttggt agaaaaattt ggtccgaaag tcatgggaag tctatctgct
    13981 tttctatacg caggtggaaa tatcttagca ggatttgcaa tagaccgtca ggagctgtgg
    14041 ttgttgtatc tcgcttatgg cattttaggt gggcttggtt tgggagcagg ctatattacc
    14101 cctgtgtcga cgattataaa atggtttcct gataaacgtg gtctcgcaac aggtttagcg
    14161 attatggggt ttggttttgc ttctttattg actagtccca tagcgcaaca cctcatcgca
    14221 ggggtagggc ttgtagaaac tttttatatt ttaggagcaa gttactttat tatcatgctc
    14281 ctagcttcac aattcattaa gcgtccaaat gagcaagagc ttgcaatttt atcttcttca
    14341 gggaaagaaa aaacagcctc tttgacgcaa ggaatggctg caaatcaggc tctgaaaagc
    14401 aatcggtttt atatgctttg gattattttc tttatcaaca tagcttgtgg tttaggctta
    14461 atttcagcgg catcgccaat ggcacaggag atggctggct tgtctacaag tcatgcagca
    14521 gtaatggtgg gtgttttggg gattttcaat ggatttggtc gcttgctctg ggcgagtttg
    14581 tctgactata tcggtcgccc tctaaccttt agtatattac tgcttgtcaa tcttttcttt
    14641 tctctctcac tttggctctt tacagattcc gttttatttg tagttgctat gtctattttg
    14701 atgacttgct atggagctgg tttttctttg attccagctt atctcagtga tatttttgga
    14761 accaaggaat tggccgctct gcatggttat attttaacag cttgggcaat ggctggttta
    14821 gcgggaccta ttttattagc agagacttat aaaatggctc attcgtacac acaaaccttg
    14881 ttcgtttttc tcattttata cagtatcgcc ttggctttgt cttattatct aggtcgttca
    14941 atcaaaaaag aaagtcaaaa accgcttaca tgattttctg tagaaatggg gctatctttt
    15001 gctctggtct tgtgttatac tagataggtt gcaaagaaaa cagtactttt cttttgtgga
    15061 aaagaagcaa cacgatttta tatctagtta tatgaaaata cgtcataaaa agaaaagtat
    15121 aaactaagcc ctatagggtg gcttcgcacc acctttagaa agaagaataa cgtgaaattt
    15181 aatgaattaa acttgtctgc tgatttgcta gcagaaattg aaaaagctgg ttttgtagaa
    15241 gctagtccta tccaagaaca aactattcct ttggcccttg aaggcaagga tgttatcggt
    15301 caagctcaga ctggtacagg aaaaactgca gcctttggct tgcctaccct tgaaaaaatc
    15361 cgtacagaag aagcgactat ccaagccttg gtcatcgctc caactcgtga actagctgtc
    15421 caaagtcaag aagaactctt ccgctttggt cgtagtaagg gagtcaaagt ccgttcagta
    15481 tatggcggat caagcattga aaaacaaatt aaggctctta aatctggtgc ccatatcgtg
    15541 gtgggaactc caggtcgcct cttggacttg attaaacgca aggccttgaa attacaagac
    15601 attgaaaccc ttatccttga cgaagcggat gaaatgctta acatgggctt ccttgaagac
    15661 atcgaagcca ttatttcccg tgtacctgag aaccgtcaaa ctttgctttt ctcagcaact
    15721 atgccagatg ccatcaaacg tatcggtgtt cagtttatga aagcccctga acatgtcaag
    15781 attgcggcta aggaattgac aacagaattg gttgaccagt actatatccg tgttaaggaa
    15841 caagaaaaat ttgacaccat gactcgtctc atggatgtgg cacaaccaga actcgctatt
    15901 gtatttggtc gtaccaaacg ccgtgtggat gaattgactc gtggtttgaa aattcgtggc
    15961 ttccgtgcag aaggaattca tggcgaccta gaccaaaaca aacgtcttcg tgtccttcgt
    16021 gactttaaaa atggcaatct tgatgttttg gttgcgacag acgttgcagc gcgtggtttg
    16081 gatatttcag gtgtgaccca tgtctacaac tacgatattc cacaagatcc tgagagttat
    16141 gttcaccgta tcggtcgtac aggtcgtgct ggtaagtcgg gtcaatctat tacttttgtt
    16201 gctccaaacg aaatgggtta ccttcaaatc attgaaaact tgactaagaa acgcatgaaa
    16261 ggtctcaaac ctgcaagtgt agaagaatcc ttccaatcaa aaaaacaggt agctctcaag
    16321 aaaatcgaac gtgattttgc agatgaaacc attcgtgcca actttgagaa atttggtaag
    16381 gatgctcgca aattggctgc tgagtttact ccagaagaat tggcaatgta tatcttgagt
    16441 ctgacagtcc aagacccaga tagccttcca gaagtggaga ttgcacgtga aaaaccacta
    16501 ccgtttaaac catcaggtaa tggtttcggt ggtaaagcta agggaggtcg tggaggccgt
    16561 cgtggggacg accgtcgaga gcgtgatcgc cgtggcaatg gtcgccgtga tgagttcaaa
    16621 aaaggaagtc gtggcaacga tcgttttgat aaggaaaaac gttaccgtaa ggataataaa
    16681 aaaccacgca atactttaag cgaaaagcaa acaggctttg ttattcgtaa caaaggcgat
    16741 aaataagaaa aagcggttca aaatgaatcg cttttttata taaggagacc gaatgtgaaa
    16801 agacaaataa aattattata ttatcttttg taatgttatg atacacaaaa ataggcaact
    16861 tgtcaataga aaaggaacaa tttaattaaa aatattctgg tttttaatta aattgtaagt
    16921 caatgcaagt caataaggaa ttttcagata attattgaaa aagagacgtt tttatgttat
    16981 aatgatagcg attaaattcg aggtgaaaaa tggcacattt attagaaaaa actagaaaaa
    17041 ttacatcaat tttgaaacgc tcagaggagc agttgcagga tgagctccct tacaacgcta
    17101 ttacgcgtca gttagcggat attattcatt gcaatgcctg cattatcaat agtaagggac
    17161 gtctgcttgg ctattttatg cgttataaaa caaatacaga tcgcgtagag caattcttcc
    17221 aaactaagat tttcccagat gactacgttc aaggggctaa tatgatttac gaaacagaag
    17281 caaacttgcc tgttgagcat gatatgagta ttttccctgt tgagagtaga gatgattttc
    17341 cagatggctt gacgactatt gcaccgattc atgtatcggg gattcgcctt ggttctttga
    17401 ttatttggcg taatgataaa aaattcgaag atgaggactt ggttcttgtt gagattgcca
    17461 gtaccgttgt tgggattcag cttcttaact tccaacgtga agaagatgag aaaaatattc
    17521 gtcgtcgtac tgctgtcacc atggcggtca ataccctttc ttactccgaa ctccgtgctg
    17581 tttcagcaat tttaggggaa ttaaatggaa atgaagggaa gttgactgcg tcagtgattg
    17641 cagatcgtat cggaatcact cgctctgtga ttgtcaatgc tcttcgtaaa cttgagtctg
    17701 cggggattat tgaaagtcgc tcacttggaa tgaagggaac ctatcttaag gtcttgattt
    17761 cagatatttt tgaagaagtg aagaaaagag attactaatg acaaaggctt taatttcgat
    17821 tgattataca gaagattttg ttgctgatag tgggaagctg acagcaggtg ctccagctca
    17881 ggcgatttcg gatgccatta gcaaggtaac tagattagct tttgaacgag gagattatat
    17941 cttctttact attgatgctc atgaagaaaa cgattgtttc catccggaaa gtaagttatt
    18001 tcctcctcat aatctgattg ggacgagtgg acggaattta tatggagatt tggggatctt
    18061 ttatcaagag catggttcag acagtcgtgt cttttggatg gataaacgcc attactcagc
    18121 tttttcaggg actgacctag atattcgttt gagagagcgt agagtgtcta ccgttatctt
    18181 aacaggtgtc ttgacggata tctgtgtcct acatacagct atagatgcct ataatctagg
    18241 atatgacatc gagattgtta aaccagctgt tgcttccatc tggcctgaaa atcatcaatt
    18301 tgccctaggt catttcaaaa atacactcgg agctaagtta gtagatgaaa atctaaatga
    18361 actttctgag taacctggtt ggtgaaaatg aaaaaatttg aaaaaaagtg ttgacaagca
    18421 tcccaaaagt tgatatacta gtaaagtaat cgacgcgggg atggcggaat tggcagacgc
    18481 gcaggactaa ggatcctgtg accgctttag gtcgtgaggg ttcaagtccc tctctccgca
    18541 taggaaaaga gttagctaaa gctagctctt ttgtatttct ctaaaaaatt agaatccagt
    18601 caaatgtagt ggagaatcag cgaaaatatg ggaaaactga tccttgtttc tgcttttgtc
    18661 atagaagaat aataaagatt atcttcttca aattctccga tattctctaa agttttgtgc
    18721 aagttgcaca gaactttttt atttttttgg tcatcttgcc atagaaatat aaagcgtttt
    18781 catctataat ataattatca aaagacaaaa ggagttcacc tcatggtaga attgaatctt
    18841 aaaaatattt acaaaaaata tccaaacagc gaacactatt cagttgaaga tttcaacttg
    18901 aacatcaaag ataaagaatt tatcgttttc gtaggacctt caggatgtgg taaatcaact
    18961 acactccgta tgattgctgg tcttgaagac attacagaag gtactgcatc tatcgatggc
    19021 gtggttgtca acgacgtagc tccaaaagac cgtgatatcg ccatggtatt ccaaaactac
    19081 gctctttacc cacacatgac tgtttatgac aacatggctt tcggtttgaa attgcgtaaa
    19141 tacagcaaag aagacattaa caaacgtgtt caagaagcag ctgaaatact tggattgaaa
    19201 gaattcttgg aacgtaaacc agctgacctt tcaggtggtc aacgtcaacg tgttgccatg
    19261 gggcgtgcga ttgtccgtga tgcgaaagta ttcttgatgg acgaaccttt gtcaaacttg
    19321 gatgccaaac ttcgtgtatc aatgcgtgct gaaatcgcta aaattcaccg tcgtatcgga
    19381 gctacaacta tctatgtaac tcacgaccaa acagaagcga tgacacttgc agaccgtatc
    19441 gttattatgt cagctactaa gaaccctgct ggtacaggta ctatcggacg tgtagaacaa
    19501 atcggtactc ctcaagaagt ttacaaaaat ccagttaaca aattcgttgc aggattcatc
    19561 ggaagcccag ctatgaactt catcaacgtg aaattggttg gtagcgaaat tgtttctgac
    19621 ggtttccgtt tgaaagtgcc agaaggagca ttgaaagttc ttcgtgaaaa aggctacgaa
    19681 ggaaaagaat tgatctttgg tatccgtcca gaagacgtga atgcagaacc tgctttcctt
    19741 gaaacattcc cagactgtgt tgtaaaagcg actatctctg tatcagaact gcttggttca
    19801 gaatctcacc tttactgcca agttggtaaa gacgagtttg ttgcaaaagt tgatgctcgt
    19861 gactacttgc aaacaggtgc aacagttgag cttggatttg acttgaacaa agcacacttc
    19921 ttcgatgtag aaactgaaaa aacaatctac taaaataaat aaaattcaaa gcactacaag
    19981 aaaagatatc tctttatcaa ttgtagtgga gagatatcag ttaatctagg gagagaaaca
    20041 aaatgcttct ctcctttttg ctagggaagt catattatgc atctatattg tgatgctctt
    20101 taatactctt cgaaaatctc ttcaaaccac gtcaacgtcg ccttgccgta cgtatgatta
    20161 ctgatttcgt cagttttatc tgcaacctca aagatgtact ttgagcagct tacggctagt
    20221 ttcctagttt gctctttgat ttccattgag tattatttgt gggtaccatc tacaagtgaa
    20281 gctatatgcg taaactacgt gagcaattga attcgaacta gagaggtaat aataaattta
    20341 tgctatagtt atggtgactt gtatgctttt gattctagtt tatcaaataa tagattagaa
    20401 ttgtcagata atatcatttt gtgttataat gaagaaaaaa cagaggtgtt caaatgtcag
    20461 aagcaggtca taagttttta gcaaaattgg ggaaaaaacg cttacgtcca ggtggaaagc
    20521 gtgccacaga ttggttaatt gcagaaggag gattttcaaa agaaaagaga atactagagg
    20581 ttgcgtgtaa taggggaact acagcaattg agttggcaca gcgttttggt tgcaagataa
    20641 ctgctgttga tatggatgct caagctttag aagtggctaa aaaatctgct ggaacggcag
    20701 gtgttgctca tttaatcagt tttgaaagag caaatgcaat gaaacttcct tatcaagatg
    20761 ctagttttga tattgttata aatgaagcta tgctgactat gcaagccgat caagctaaga
    20821 aaaaatgtgt aatggaatat ctaagggtat taaaacctgg aggtcttctc ttgacacatg
    20881 atgtgcttct taaggaagct aaagagtcta tcagacagga attatcacaa gcaattcatg
    20941 taaatgtagg tcctttaact caagatggtt gggaacaggt gatgatagaa tcaggttatt
    21001 gtgatgtgaa agcattgact ggtgaaatga cattaatgaa attatcgggt atgatttatg
    21061 acgaaggttt gctaggaact ttgaaaattt gtgtaaatgc ttgtaaaaag gagaatagaa
    21121 agcagttttt aactatgtat aaaatgtttg ctaagaataa acagaaattg ggctttattg
    21181 cgatggctag ttataaatcg tcaaaacgtt agataattat tgaagttaac ttttcctttt
    21241 ttctttctta aaaaatatgc tataatagag agtaaaaaac tttgaaagaa agaaaaaaga
    21301 tgaatttaaa agattacatt gcaacaattg aaaattatcc aaaggaaggc attaccttcc
    21361 gtgatattag tcctttgatg gctgatggaa atgcttatag ctacgctgtt cgtgaaatcg
    21421 ttcagtatgc tactgacaag aaagtcgaca tgatcgtggg acctgaagct cgtggattta
    21481 tcgtgggttg tccagttgcc tttgagttgg gaattggttt tgcgcctgtt cgtaagccag
    21541 gtaaattgcc acgtgaagtt atttctgctg actatgaaaa agagtacggt gtcgatacct
    21601 tgactatgca cgcggatgcc attaagccag gtcaacgtgt tcttattgta gatgaccttt
    21661 tggcgacagg tggaactgtt aaggcaacta tcgagatgat tgaaaaactt ggtggtgtta
    21721 tggcaggttg tgccttcctt gttgaattgg atgaattgaa cggccgtgaa aaaattggtg
    21781 actacgacta caaagttctt atgcattatt aatgaaaaca gtccctaggg ctgttttctc
    21841 tacactatga tataaaaata gactataact agttagagaa aaactataat tgaaaactat
    21901 atcttcttgc agtataataa aaggactaag tgtttgagat ttgtcttcaa acatatgcaa
    21961 ttattcctga aagagtacag ttaggagagg gttatgccga ttcgaattga taaaaaattg
    22021 ccagctgttg agattttacg gacagagaat atctttgtca tggatgatca acgtgctgcc
    22081 caccaagata tccgtccttt gaagatttta attttaaatc tcatgccaca gaaaatggtc
    22141 acagagaccc agttgttgcg ccacttggct aatacacccc tacaactgga tattgatttt
    22201 ctctatatgg agagccaccg ttctaaaaca actcgttcag agcacatgga gaccttctat
    22261 aaaacttttc ctgaagtcaa ggatgagtat tttgatggga tgatcatcac gggtgctcca
    22321 gttgagcatt taccatttga ggaagtggac tattgggagg aatttagaca gatgcttgag
    22381 tggtctaaga ctcatgtcta ttcgaccctt catatctgtt ggggggctca ggctgggctt
    22441 tatctgcgct atggtgtaga aaaataccag atggacagta agctatcagg tatttatcct
    22501 caggacaccc taaaagaggg tcaccttcta tttagaggct ttgatgatag ctatgtatcc
    22561 cctcattcac ggcacacgga gatttctaag gaagaggtct taaacaagac caatctcgag
    22621 attttatcag aaggacctca ggttggggtt tctattttgg ccagtcgtga tttacgagaa
    22681 atttatagtt ttggtcattt ggagtatgac cgtgatacct tggcaaaaga gtattttcga
    22741 gatcgtgatg caggtttcga cccacatatt ccagaaaatt actttaaaga tgatgatgtt
    22801 aatcaggtac cttgtctttg ttggtcttca tctgcagccc tctttttcag taattgggta
    22861 aaccatgcgg tctatcagga gacgcctttt gattggagaa agatagaaga tgatgcatct
    22921 gcatatgggt atttataaga ggaattatga catatttaga cgcttttaaa tcaggtacct
    22981 tggttttacc gagtgccctg ctcttgcatt ttaaggaact ctttccttct agcgacgatt
    23041 ttctggtttg gcaatttttc tatttgcaaa atacgacagg cttagaagaa atgtcgccaa
    23101 gccagattgc tgaaaggatt ggcaaggaaa tttcggatgt caaccagtcc atttctaatc
    23161 tgacggaaag gggactgctc cagtatcgta ctatcgaatt aaatggcgaa attgaattgc
    23221 tctttgatgc tagtttggcc ttggaacgtt tggatgacct gtttggagca gttcattcaa
    23281 gttcagacca gctaacatct caaaaccagc tcaaggattt ggtggaaacc ttccagcagg
    23341 agttgggacg attgttgacg ccttttgaga ttgaggattt gaccaagaca ctaaaggaag
    23401 atggaaccag tgctgacttg attaaggagg ctcttcgtga agctgttttg aatggaaaac
    23461 caaactggaa gtacattcag gcgattttga gaaactggcg ccatgaagga atcaagagtg
    23521 tggctcaaat tgaggccaag agagcagaaa gagaagcaag caatcctcag ttgacacagg
    23581 tatctgcaga tttcagaaat gccatggatc tctggaagga ttaatccatg caagtaggct
    23641 tgaaatccga gtaagatttg caagctgtgt ataattgtga tagaataaat agaaaataaa
    23701 ttgaaaaaag aggtatgtga aatgtcacgt aaaccattta tcgctggtaa ctggaaaatg
    23761 aacaaaaatc cagaagaagc taaagcattc gttgaagcag ttgcatcaaa acttccttca
    23821 tcagatcttg ttgaagcagg tatcgctgct ccagctcttg atttgacaac tgttcttgct
    23881 gttgcaaaag gctcaaacct taaagttgct gctcaaaact gctactttga aaatgcaggt
    23941 gctttcactg gtgaaactag cccacaagtt ttgaaagaaa tcggtactga ctacgttgtt
    24001 atcggtcact cagaacgccg tgactacttc catgaaactg acgaagatat caacaaaaaa
    24061 gcaaaagcaa tctttgcgaa cggtatgctt ccaatcatct gttgtggtga atcacttgaa
    24121 acttacgaag ctggtaaagc tgctgaattc gtaggtgctc aagtatctgc tgcattggct
    24181 ggattgactg ctgaacaagt tgctgcctca gttatcgctt atgagccaat ctgggctatc
    24241 ggtactggta aatcagcttc acaagacgat gcacaaaaaa tgtgtaaagt tgttcgtgac
    24301 gttgtagctg ctgactttgg tcaagaagtc gcagacaaag ttcgtgttca atacggtggt
    24361 tctgttaaac ctgaaaatgt tgcttcatac atggcttgcc cagacgttga cggtgccctt
    24421 gtaggtggtg cgtcacttga agctgaaagc ttcttggctt tgcttgactt tgtaaaataa
    24481 tcagtaagta gcaaaagcta ggtggaacag cattcagatg tctgttacat tttttatagg
    24541 agagcaaaga ttgaaaacaa aaattggatt agcaagtatc tgtttactag gcttggcaac
    24601 tagtcatgtc gctgcaaatg aaactgaagt agcaaaaact tcgcaggata caacgacagc
    24661 ttcaagtagt tcagagcaaa atcagtcttc taataaaacg caaacgagcg cagaagtaca
    24721 gactaatgct gctgcccact gggatgggga ttattatgta aaggatgatg gttctaaagc
    24781 tcaaagtgaa tggatttttg acaactacta taaggcttgg ttttatatta attcagatgg
    24841 tcgttactcg cagaatgaat ggcatggaaa ttactaccta aaatcaggtg gatatatggc
    24901 ccaaaacgag tggatctatg acagtaatta caagagttgg ttttatctca agtcagatgg
    24961 ggcttatgct catcaagaat ggcaattgat tggaaataag tggtactact tcaagaagtg
    25021 gggttacatg gctaaaagcc aatggcaagg aagttatttc ttgaatggtc aaggagctat
    25081 gatgcaaaat gaatggctct atgatccagc ctattctgct tatttttatc taaaatccga
    25141 tggaacttat gctaaccaag agtggcaaaa agtgggcggc aaatggtact atttcaagaa
    25201 gtggggctat atggctcgga atgagtggca aggcaactac tatttgactg gaagtggtgc
    25261 tatggcgact gacgaagtga ttatggatgg tgctcgctat atctttgcgg cctctggtga
    25321 gctcaaagaa aagaaagatt ttaatgtcgg ctgggttcac agagatggta aacgctattt
    25381 ctttaataat agagaagaac aagtgggaac cgaacatgct aagaaaatca ttgatattag
    25441 tgagcacaat ggtcgtatca atgattggaa aaaggttatt gatgagaacg aggtggatgg
    25501 tgtcattgtt cgtctaggtt atagcggtaa agaagacaag gaattggcgc ataacattaa
    25561 ggagttaaac cgtctgggaa ttccttatgg tgtctatctc tatacctatg ctgaaaatga
    25621 gaccgatgct gagaatgacg ctaaacagac cattgaactt ataaagaaat acaatatgaa
    25681 cctgtcttac cctatctatt atgatgttga gaactgggaa tatgtaaata agagcaagag
    25741 agctccaagt gatacagaca cttgggttaa aatcatcaac aagtacatgg acacgatgaa
    25801 gcaggcgggt tatcaaaatg tgtatgtcta tagctatcgt agtttattac agacgcgttt
    25861 aaaacaccca gatattttaa aacatgtaaa ctgggtagcg gcttatacga atgctttaga
    25921 atgggaaaac ccttattatt caggagaaaa aggttggcaa tatacctctt ctgaatacat
    25981 gaaaggaatc caagggcgcg tagatgtcag cgtttggtat taagcgatga tttgaaagag
    26041 ggatgtgata gtagcaccct ctttttcttt gttttatgat agttcatcct cgagtaaatt
    26101 caagatcttg ctcggaaatg aagcttatat agtagattga atatagacaa ataccttgtg
    26161 attggtaaaa cattttagaa attcatttac ctttcctaat cgacttggtt tcatcttatt
    26221 tcaatctatt atagtattgg ggaatttctt caaaccacat cagcttggtc agttctacct
    26281 gcgacctcaa aacttgtgct ttggtcaagc tgggtttagt ttcctagttt gctgatggat
    26341 ttccattgac tataagcatc caaccctctt tttgtcttct aaagaattct taaattatca
    26401 gtctattgca acttttctca tataagttct ttgtcttgct attggttttc cttagtagta
    26461 tactaaggta gtaatcatta agaagtggtt acaaaaaata atgaatgagg taaaataaaa
    26521 tggtagaatt gaaaaaagaa gcagcaaaag atgtcgcaac attgacaaaa gcagcgccag
    26581 tagcattggc aaaaacaaag gaagtcttga accaagctgt tgctgatttg tatgtagctc
    26641 acgttgcttt gcaccaagtg cactggtata tgcatggtcg tggtttcctt gtatggcatc
    26701 caaaaatgga tgagtacatg gaagctcttg acggtcaatt ggatgaaatc agcgaacgct
    26761 tgattacact cggtggaagc ccattctcta ctttgacaga gttccttcaa aacagtgaaa
    26821 tcgaagaaga agctggtgaa taccgtaatg ttgaagaaag cttggaacgt gttctagcta
    26881 tttatcgcta cctcatcact cttttccaaa aagcgttgga tgtgactgac gaagaaggcg
    26941 atgatgttac aaacgatatt tttgttggtg ctaaggctga acttgagaaa acagtttgga
    27001 tgcttgccgc agaacttgga caagcacctg gtttgtaaga aacaagacaa catagataaa
    27061 tctgtaggaa atttcttaca gattttttct attttgtaag ctattccaaa ccagtctttt
    27121 tttgagtttt ttgataaaat agtactatca gtgaaaagga tggaagcatg actaagaaaa
    27181 tcgtagctat ttgggcccag gatgaagagg gtgtgattgg taaggacaat cgtctgcctt
    27241 ggcatttgcc agcagaattg cagcacttta aagaaacaac tctgaatcat gctatcttga
    27301 tggggcgggt aacctttgat ggaatggggc gtcgcttgct tccaaaacgg gagaccctga
    27361 ttttgacgcg taatccggaa gaaaagatag atggggttgc tacttttcag gacgtccagt
    27421 ctgttcttga ctggtatcag gctcaagaaa agaatctcta cattatcggt gggaagcaaa
    27481 tttttcaggc ttttgaagct taccttgatg aagtgattgt cactcacatt catgctcggg
    27541 tggaaggaga tacctatttc cctgaagagc ttgacttgtc tctttttgag acagtttcaa
    27601 gcaaatttta cgccaaagat gagaagaatc cttatgattt taccatccaa taccgcaaga
    27661 gaaaggaagt ttaatggaac ggagtttatt tggtctattc acggcttttc tgtgtttcat
    27721 ctgctttctg gcaggagcac aagcttttcg taaaaagcgt tacgggcttt ctatcctcct
    27781 ttggttaaat gcctttacca atctggtcaa tagtattcat gctttttaca tgaccttatt
    27841 ttagatagaa tgacagtata gaatggaagg aaatcatgtc tacaaataga aaaaatgata
    27901 tgatggttta ttgctcattt tgtggcaaaa accaagaaga agtacaaaaa ataattgctg
    27961 gcaacaatgc ttttatttgt aatgaatgcg tggagttagc tcaggaaatc attcgagaag
    28021 aattggttga ggaagtcttg gcagacttgt ctgaggtgcc aaaaccaatt gaactcctcc
    28081 atatcttgaa ccactatgta attggtcaag atcgtgccaa gcgtgccttg gcagtggcgg
    28141 tttataacca ctacaaacgc atcaatttcc acgatacacg cgaagagtca gaagatgtgg
    28201 atttgcagaa gtcaaacatt ttgatgattg gcccaactgg ttcagggaaa actttccttg
    28261 cccagacctt ggctaagagc ttgaatgtac cttttgctat tgcggatgcg acagctctga
    28321 cggaggctgg ttatgtgggt gaggatgtgg aaaatatact cctcaaactc ttgcaggttg
    28381 ctgactttaa catcgaacgt gcagagcgtg gcattatcta tgtggatgaa attgacaaga
    28441 ttgccaagaa gagtgagaat gtgtctatca cacgtgatgt ttctggtgaa ggggtgcaac
    28501 aagcccttct caagattatc gagggaactg ttgctagcgt accgcctcaa ggtggacgca
    28561 aacatccaca acaagagatg attcaagtgg atacaaaaaa tatcctcttc atcgtgggtg
    28621 gtgcttttga tggtattgaa gaaattgtca aacaacgtct gggtgaaaaa gtcatcggat
    28681 ttggtcaaaa caataaggcg attgacgaaa acagctcata catgcaagaa atcatcgctg
    28741 aagacattca aaaatttggt attatccctg agttgattgg acgcttgcct gtttttgcgg
    28801 ctcttgagca attgaccgtt gatgacttgg ttcgcatctt gaaagagcca agaaatgcct
    28861 tggtgaaaca ataccaaact ttgctttctt atgatgatgt tgagttggaa tttgacgacg
    28921 aagcccttca agagattgct aataaagcaa tcgaacggaa gacaggggcg cgtggacttc
    28981 gctccattat cgaagaaacc atgctagatg ttatgtttga ggtgccgagt caggaaaatg
    29041 tgaaattggt tcgcatcact aaagaaactg tcgatggaac ggataaaccg atcctagaaa
    29101 cagcctagag gtgactatgg aacttaatac acacaatgct gaaatcttgc tcagtgcagc
    29161 taataagtcc cactatccgc aggatgaact gccagagatt gccctagcag ggcgttcaaa
    29221 tgttggtaaa tccagcttta tcaacactat gttgaaccgt aagaatctcg cccgtacatc
    29281 aggaaaacct ggtaaaaccc agctcctgaa cttttttaac attgatgaca agatgcgctt
    29341 tgtggatgtg cctggttatg gctatgctcg tgtttctaaa aaggaacgtg aaaagtgggg
    29401 gtgcatgatt gaggagtact taacgactcg ggaaaatctc cgtgcggttg tcagtctagt
    29461 tgaccttcgt catgacccgt cagcagatga tgtgcagatg tacgaatttc tcaagtatta
    29521 tgagattcca gtcatcattg tggcgaccaa ggcggacaag attcctcgtg gtaaatggaa
    29581 caagcatgaa tcagcaatca aaaagaaatt aaactttgac ccgagtgacg atttcatcct
    29641 cttttcatct gtcagcaagg cagggatgga tgaggcttgg gatgcaatct tagaaaaatt
    29701 gtgaggaaaa gaaaatggca aaaacaattc atacagataa ggccccaaag gctatcggac
    29761 cctatgttca aggaaaaatc gttggcaacc ttttgtttgc tagcggtcaa gttcccctat
    29821 cccctgaaac tggggaaatt gtaggagaga atatccaaga acagacagag caagtcttga
    29881 aaaacatcgg tgctattttg gcagaagcag gaacagactt tgaccatgtt gtcaaaacaa
    29941 cttgtttctt gagcgatatg aacgactttg ttccttttaa tgaggtttac caaacggcct
    30001 tcaaagagga attcccagct cgttcagctg tggaggtagc tcgtcttcct cgtgatgtaa
    30061 aagtcgaaat tgaagtcatc gcagagattg gataagctag ttgaagtttg gtgttgccaa
    30121 acttcttttg atataaggag aaaaagatga caaagaaaca acttcacttg gtgattgtga
    30181 cagggatgag tggcgcaggg aaaactgtag ccattcagtc cttcgaggat ctaggttatt
    30241 tcaccattga taatatgccg ccagctctct tgcctaagtt tttgcagctg gttgaaatta
    30301 aggaagacaa tcctaagttg gccttggtag tggatatgcg tagccgttct ttcttttcag
    30361 agattcaagc tgttttggat gagttggaaa atcaagatgg tttggatttc aaaatcctct
    30421 ttttggatgc ggctgataag gaattggtcg ctcgttacaa ggaaaccaga cggagtcacc
    30481 cactagcagc agacggtcgt attttagatg gaatcaagtt ggaacgtgaa ctcttggcac
    30541 ctttgaaaaa tatgagccaa aatgtggtgg atacgactga actcactcca cgtgagctgc
    30601 gcaaaaccct tgcagagcag ttttcagacc aagaacaagc ccagtctttc cgtatcgaag
    30661 tcatgtcttt cggatttaag tatggaatcc cgattgatgc ggacttggtc tttgatgtcc
    30721 gtttcttgcc aaatccctat tatttaccag aactgagaaa ccaaacgggt gtggatgaac
    30781 ctgtttatga ttatgtcatg aaccatcctg agtcagaaga cttttatcaa catttattgg
    30841 ccttgattga gccgattctg ccaagttacc aaaaggaagg taagtccgtt ttgaccattg
    30901 ccatgggatg tacgggtgga caacaccgta gtgtggcatt tgctaaacgc ttggtgcagg
    30961 acttatccaa gaattggtct gttaatgaag ggcatcgcga caaagaccgc agaaaggaaa
    31021 cggtaaaccg ttcatgagaa aaccaaagat aacggtgatt ggtggaggga ctggaattcc
    31081 cgtcattcta aaaagtctgc gggaaaaaga tgtggaaatc gcagctatcg tgacggtggc
    31141 agatgatggt ggttcttcag gtgaactccg aaaaaatatg caacagttga caccgccagg
    31201 tgattttcgt aatgtccttg tggccatgtc ggatatgcct aagttttatg agaaggtctt
    31261 tcagtatcgg ttctctgagg atgccggagc ctttgctggc catccattgg gaaatctcat
    31321 cattgctggc ttgtcagaaa tgcagggttc aacctataat gccatgcagt tattgagcaa
    31381 atttttccat acaacaggga aaatttatcc ttccagtgac catcctttga cccttcatgc
    31441 agtctttcag gatgggacag aagtggctgg agagagtcat attgtagacc atcgaggcat
    31501 aattgacaat gtctatgtga ccaatgccct aaacgatgat acgcctctgg ccagccgtcg
    31561 agtagtgcag accatccttg aaagtgacat gattgtccta gggccaggtt ccctctttac
    31621 ctctattttg cccaatatcg tgattaagga aattgggcgg gctcttttgg aaaccaaggc
    31681 agaaattgcc tatgtctgca atatcatgac ccaacgtggg gagacggaac actttacaga
    31741 tagcgaccac gtggaagtct tgcatcgtca ccttggtcgc ccttttatcg acactgtctt
    31801 ggtgaatatt gaaaaagtgc ctcaggaata catgaattcc aaccgttttg atgaatactt
    31861 ggtgcaagtg gaacacgatt ttgtaggtct ttgtaagcaa gtttcgcgcg tgatttcatc
    31921 taacttcctt cgtctggaaa atggcggtgc cttccacgat ggagatttga ttgtggacga
    31981 gttgatgcgc attatacagg tgaaaaaatg agtttcacag tagcagtaaa agaagaaatc
    32041 ctgggccaac accatctgag ctggcatgaa ttatctgcca ttatcaaaat gtctggtagc
    32101 atcggtctct cgacttcggg cttgactttg tcagttgtga cagaaaatgc caaactggcc
    32161 cgtcacctct atgagtcctt tctccatttc tatgaaatca aatctgaaat tcgtcaccac
    32221 caacggagca atcttcgcaa gaatcgcgtc tataccgttt ttacagatga aaaggtgcag
    32281 gatttgttaa gtgatttgca cttggcagac tctttctttg gcctggaaac aggtattgat
    32341 gaggcgattt tatcggatga ggaagcaggt cgtgcctatc tctgtggcgc tttcttggca
    32401 aatgggagca ttcgtgaccc tgagtcaggc aagtaccagt tggaaatcag ttctgtttat
    32461 ctggaccacg cgcaaggaat tgcctccctt ctccaacagt ttttactgga tgccaaggtg
    32521 cttgagcgca agaagggggc tgtgacctat ctccagcgag cagaagacat tatggacttc
    32581 ttgatagtaa tcggagccat gcaggcacgt gatgattttg agcgggttaa gattttgcga
    32641 gaaacccgta acgacctcaa tcgggctaat aatgccgaga cagctaatat cgctcggaca
    32701 gtttctgcca gcatgaagac cattaacaat atcagtaaaa tcaaagatat catgggctta
    32761 gaaaatttgc cagtagattt gcaggaagtg gcgcaactga ggattcagca cccagactac
    32821 tctatccagc agttagcaga tagtctcagc acccctctga ccaaaagtgg tgtcaatcac
    32881 agactcagaa aaatcaataa gatagcggat gaattataaa accacgaatc ctatgtgact
    32941 cgtggttctt ttttataaac tggtagagtg ttttggttgt actttttgtt cagggtaaat
    33001 gtagttgata gcgttgttga cagcagttgg agcttctccg aggcctgtcg caatcagatc
    33061 aatttttccg tcatagtagc agcagtcacc gatagcatag atacctgctt ggctggattc
    33121 ctgtttgctg tttacgataa tcttgtgacg gttgaggtcg agcccccagt ttttaaggtt
    33181 accgacagaa gatttgaaac catagttgac aaagaggtgg tctaggtcaa tggtttcagt
    33241 ttcatcagat ttgacttttg tgatttcaag tttatcaagt gtttttccat ttccaaggag
    33301 ttggctaggg gcgaatggtg tcttgatggt tacagatgat tcttgcaagg cttgaacact
    33361 gtgttccaag gcacggaaat tatctctgcg gtgaacaagg gtagttggtg cgattttttc
    33421 aaaagccaaa gtccaatcca cagccgagtc tcccccgcca agaatcgtca ctttcttacc
    33481 agcgtattgc tgaatgttag aaacgtggta gtggatattt tcatagccct caaccccttc
    33541 aagttccagc ggacgtggtt tgaaggcacc gccacccata gcaatgataa ctgttttagt
    33601 caggtgactt cctttagaag ttgtgatggc aaattcttct tcttgtttgt caatttcaag
    33661 aaccgtttca ttgagatgaa taggggtatc aaatccattt agctgttcaa tcaagcggtt
    33721 agtcaactct tctccagtca ggtttgggaa gcctggtacg tctaggattt ccttttcagg
    33781 gtagagaata gcaggttgtc cacctagctg gggaagagag tcgatgattt gaaccttggc
    33841 ttggcgtagg tgggcataaa aggctgcaaa aagcccgaca ggaccaccac ccacaatggt
    33901 aatatcatag agttgagaca tagtttctcc tttgtttttt ctagtcagtt tattttatca
    33961 tatttttgtt caatgtaaaa tggactagga tagggaaaaa aatgccagaa aatggtataa
    34021 taggacgaaa gtgttttgat agggaggata tcagggatga attttcaaca attatccaat
    34081 ctgcaatatt ggaccagttt gtttgcaagt ccatggacga tagctatcaa tctgattgat
    34141 attttgattg ttgcttatat tttataccat tttacaaaag ctattgcagg aaccaagatt
    34201 atgattttgg tacgtggagt tttggtgttt attttagctc aaatccttgc aaatatgatt
    34261 ggtttgacta cgatttcttg gttaatcaat caaattatta cttatggggt tattgcggcg
    34321 gttgttatct tctctccaga gattcggact ggtttggaac gtttgggaag agcgacagat
    34381 ttcttttcca atgcccctat tagtgctgag gaacagatga ttcgtgcctt tgttaagtct
    34441 gttgaataca tgagtcctcg taaaatcggg gccttggttg ctattcagcg tgtacgtacc
    34501 ttgcaggagt atatgtcgac aggaattccc ttggatgcta agatttctgc agaacttctc
    34561 attaacattt ttattcccaa cactccccta catgacggtg cggtgattat caaagaagaa
    34621 cgtatcgctg tgacgtctgc ctatctgccc ttgacaaaaa acacagggat ttccaaggaa
    34681 tttgggacca gacaccgggc ggctatcggt ttatcagaag tctcagatgc cttgactttt
    34741 gtcgtatcag aggaaacggg aggaatttcg ataacctata atggaaggtt taagcacaac
    34801 ctaacacttg atgaatttga aacagaatta cgtgaaatct tacttccaaa agaggaagtg
    34861 ggtcttagtt ttaaagaacg attgctagga ggatggaaac atgaaaaaaa atagtttata
    34921 tatcatatcc tcactctttt ttgcttgtgt cttatttgtc tatgctacgg cgacgaattt
    34981 tcaaaacagt accagtgcta ggcaggtaaa aacggaaacc tatactaata cagtaacaaa
    35041 tgtccctatt gacatacgct ataatagtga taagtatttt attagcggtt ttgcttcaga
    35101 agtatcagtg gtcttgactg gtgcaaatcg tctatcgcta gctagtgaaa tgcaagaaag
    35161 tacacgtaaa ttcaaggtta ctgctgacct aacagatgcc ggtgttggaa cgattgaagt
    35221 tcctttgagc attgaagatt tacccaatgg gctgaccgct gtggcgactc cgcaaaaaat
    35281 tacagtcaag attggtaaga aggctcagaa ggataaggta aagattgtac cagagattga
    35341 ccctagtcaa attgatagtc gggtacaaat tgaaaatgtc atggtgtcag ataaagaagt
    35401 gtctattacg agtgaccaag agacattgga tagaattgat aagattatcg ctgtcttgcc
    35461 aactagcgaa cgtataacag gtaattacag tggttcagta cctttgcagg caatcgaccg
    35521 caatggtgtt gtcttaccgg cagttatcac tccgtttgat acaataatga aggtgactac
    35581 aaaaccagta gcaccaagtt caagcacatc aaattcaagt acaagcagtt catcggagac
    35641 atcttcgtca acgaaagcaa ctagttcaaa aacgaattaa aaatagaaaa ggattttata
    35701 acaatgggta aatattttgg gactgatgga gtccgtggag aagctaacct agaactaaca
    35761 ccagaattag cctttaaact aggacgtttt ggaggctatg ttcttagtca acatgaaacg
    35821 gaagcgccga aagtctttgt aggacgtgac acacgtattt caggggaaat gctggaatcg
    35881 gccttggtgg caggtctcct ttcagtaggg attcacgtat acaaacttgg tgtccttgca
    35941 acaccagcag tagcttactt ggttgaaact gaaggagcaa gtgccggtgt catgatttct
    36001 gctagccaca acccagccct tgataacgga atcaagttct ttggcggtga tggcttcaaa
    36061 ctagatgatg aaaaagaagc agaaattgaa gccttgctag atgctgagga agacactctt
    36121 cctcgtccaa gtgcagaagg tttaggaatt ttggtagatt atccagaagg cttgcgtaag
    36181 tatgaaggat accttgtttc aactggaact cctcttgatg gaatgaaggt tgccttggat
    36241 acagctaatg gagcagcttc taccagtgcc cgtcaaatct ttgcagacct tggtgcccaa
    36301 ttgacggtta tcggggaaac accagacggt cttaacatca accttaatgt tggttcaaca
    36361 catccagaag cccttcaaga agtggtcaaa gaaagtgggt cagctattgg tttggccttt
    36421 gatggagaca gtgaccgctt gattgctgtt gatgagaatg gtgacatcgt cgatggtgac
    36481 aagattatgt acatcatcgg aaaatacctt tctgaaaaag gacaattggc tcaaaataca
    36541 attgtgacaa ctgttatgtc taaccttggt ttccacaagg ccttgaatcg cgaaggtatt
    36601 aacaaggcag ttactgcagt tggtgaccgc tacgttgttg aagaaatgag aaaatcaggt
    36661 tacaaccttg gtggtgaaca gtctggtcac gttatcttga tggattacaa taccacaggt
    36721 gatggtcaat tatcagcagt tcaattgact aaaatcatga aggaaactgg taagagctta
    36781 tcagagttgg cggcagaagt aacgatttat ccacaaaaat tagttaatat ccgagtggaa
    36841 aacgtcatga aggaaaaggc catggaagtg ccagctatca aggccatcat cgagaagatg
    36901 gaagaagaaa tggcggggaa cggccgtatc cttgttcgtc caagtggaac agaacccctc
    36961 ttgcgtgtta tggcagaagc gcctacaaca gaagaagtag actactatgt tgataccatc
    37021 acagatgtag ttcgtgctga aattgggatt gactaaatcc tagaaaacta gatgaaaata
    37081 aaaaattatg gtacaatagc ttataatatt gtagccgatg gagaaagaca tgaatcttaa
    37141 acgtgaacaa gaatttgtta gtcagtatca ttttgatgcg cgtaattttg aatgggaaaa
    37201 tgaaaatgga gctcctgaaa ccaaggtgga tgtgaatttc caattgctcc aacatgacca
    37261 agaaaatcaa gtgacttcct tgattgtcat cttgagcttt atgattgtat ttgataagtt
    37321 tgtcattagt ggaacaatct cacaagtaaa ccatatcgat ggccgtattg tcaatgaacc
    37381 aagtgaattg aaccaagaag aggtggaaac cttggctcgt ccatgtttga acatgctcaa
    37441 tcgtttgact tacgaagtga ctgaaattgc attagactta ccaggaatta atttggagtt
    37501 ctaatatgaa attagctgtt ttcacagatt cctctgctta tctcagtgca gagaccttgc
    37561 aaagagaaga cttgtttgtc ttggatattc ctgtcaatat tgatggtgag gaatatgtcg
    37621 aaggcatcaa tctgtctgct gaggaatttt accaaaaaat ggctcaggct tctgaattgc
    37681 ctaagaccag tcaaccaagt attgccaagt tagatgaaat cttaacttcg ctcaaagaac
    37741 aaggctatac acatgccttg gggcttttcc tatcttctgg aatttcaggt ttttaccaaa
    37801 atatccagta tatggtcgat gactatgagg gcttaaccat tgctttccca gacactttga
    37861 ttacaagtgc tcccctaggc atcatggttg aaagcgtctt taattggcgt gatcagggcg
    37921 atgattttgc cagcattcag gataagctag ccattcaaat cagccgtacg tcagccttta
    37981 tcatggtaga tgacttggat catttggtaa aaggtggacg cctttcaaat ggggctgcca
    38041 ttttgggcaa tttgcttagc attaagccaa tcctttattt taacgatcaa ggtgtgattg
    38101 aagtttacga aaaagttcgt actgaaaaga aggccaccaa acgcttaatt gaaattatca
    38161 aggaaacaac ggcttcaggt caataccggg tcattgtcat tcatgggaat gctcctgaaa
    38221 aggctgaaga attgcgtcag catttgcttg attttggctt gggttcggat gtttcacttg
    38281 ctacatttgg tagtgtcatt ggaacgcacc taggagcagg aagtattgct ctgggttata
    38341 ttccagtgat ttagtaggca cttctaggct agttaatctt agcaattgaa cacggactac
    38401 ctgcctcttg aaaaaagatg tctgtcttgc ctttcgtgga aagtcagtgc cattccctat
    38461 ttttcatggg tggctaagat cctttgtatc ttgataattg aacacgcctg gaaccctgtg
    38521 tgaaaaagat agttcttcca aggagtagac acttcttgat cagaattcct attttcactt
    38581 tgtgttctta caggctttgt atcttagaca ggagtagaga tgagtattcg agtaattatt
    38641 gccggtttta agggaaagat gggtcaagct gcttgccaga tggtcttgac tgattcagat
    38701 ttggacttgg tagcagtttt ggatcctttt gagtctgagt cagaatggca gggtattcct
    38761 gttttcaagg ataaggctga tttagctggt tttgaagcgg atgtctgggt agactttact
    38821 actccagctg ttgcctacga aaatacacgt tttgctcttg aaaatggctt tgctccagta
    38881 gttggaacga ctggtttcac gagtgaagaa attgcagagc taaaagaatt ttctcgtgcc
    38941 caagacttgg gtggcctgat tgcccctaac tttgccttgg gtgctgtctt actcatgcaa
    39001 tttgcgacgc aggctgccaa atatttccca aatgtggaga ttattgagct ccatcatgac
    39061 aagaaaaagg atgctccgag tggaacagcc attaaaacag ctgagttgat ggcagaggtt
    39121 cgagagtcaa ttcagcaagg tgcagcagat gaggaagagc tgattgctgg tgctcgtggt
    39181 gctgactttg atggtatgcg catccactca gttcgtttgc caggtttggt agcccatcag
    39241 gaagtcatct ttggcaatca aggagaagga ttgaccctcc gtcatgactc ctatgatcgc
    39301 atctccttca tgacaggagt caatttggga attaaagaag ttgtcaagcg tcatgagctt
    39361 gtctatggat tagaacactt attatgagat taacgcaaat gccttctgaa tttcagaagg
    39421 ctttaccagt attagaaaaa attaaagaag caggctttga ggcttatttt gttgggggct
    39481 ctgttcgaga tgcccttctc catagcccta tccacgatgt ggatattgcg acgtcttctt
    39541 atccagaaga gaccaagcag atttttccgc gaacagccga tatcggaatc gagcatggaa
    39601 ccgtcttggt cttagatggg gatgaggagt atgaggtaac aacctttcgg acagaggatg
    39661 tctatgtgga ctatcgcaga cccagtgcgg tttcctttgt gcgctcgcta gaagaagacc
    39721 tcaaacgccg tgatttcaca gtcaacgcct ttgccttgga tgagacagga gaaatcgttg
    39781 acttgttcca tggtttagaa gatttggaaa agcaagtctt gcgagcagtt ggagtggcta
    39841 gtgagcgttt caacgaagat gctttacgga ttatgcgtgg tttccgtttt caggctagtc
    39901 ttggttttgc acttgagcca gaaacattta aagctatgaa gaccttgacg ccgcttttgg
    39961 agaaaatttc tgtagagcgt accttcgtcg agtttgataa actcttgctg gctccatttt
    40021 ggagaagggg cttggcttcc atgattgaga gtcaagctta tgactatctc cctgatatgg
    40081 catctagcca gaacaagctc aacagactgt ttgatttgga gactgatttt acttttgaat
    40141 cctctgaaca agcctgggcg gctttactat gggctttgga gattgaaaat gcgcagtcat
    40201 ttttgaaatc ttggaagacc tcacgccagt ttgccaagca agttcaggat ttgctgatta
    40261 ttttggctct gcgtgaaaat ggagaattga gcaagcgaga ttgttatcgc tttgacatag
    40321 atttgctttt acaggctgaa aatcttcgtc aagctcgagg aaaagaagtc aacccacaag
    40381 ccatcacaga aaaataccaa agcttgacta tccatgacaa gaaagatatt cagattaatg
    40441 gcgatatttt gatcaaggaa tatggctatc agccaggccc agacttggga gagattttaa
    40501 cagagattga gtttgccatt gtcgatggag aattggagaa taatcgtgaa gccatccatg
    40561 cttacctgag ggagaaaaaa tgagtgattt tatcgttgaa aaactaagta aatccgttgg
    40621 tgacaagacc gtttttaggg atatttcctt tattatccat gacttagaca gaattggttt
    40681 aatcggtgtc aatgggactg gcaagaccac ccttttggac gtcctttctg gtgtttctgg
    40741 ctttgatggg gatgtcagtc ctttttcagc taaaaatgat taccagattg gttacttgac
    40801 tcaggatcct gattttgatg atagaaagac agttttggat acggttctat ctagtgaact
    40861 caaggaaatc cagctcattc gtgagtatga attgattatg ctcgactata gtgaggacaa
    40921 gcaggcgcgt ttggaacgtg tcatggcaga gatggactct ctccaagctt gggaaatcga
    40981 gagtcaggtc aagaccgttc ttagcaaatt gggcattcaa gacttatcta ctcctgttgg
    41041 ggaattgtca ggtggtctga gaagacgggt acagttggca caagtcttac ttggtaacca
    41101 cgacctcttg cttttggatg agccgaccaa ccatctggat attgcgatta ttgagtggct
    41161 gaccctcttt ttgaaaaatt ctaagaagac cgtccttttt atcactcacg atcgttattt
    41221 cttagacgct ttgtcaacac ggattttcga gttggatcgt gcaggcttga ccgagtaccg
    41281 gggaaattac caggactatg ttcgcctaaa ggcggaacag gatgagcgcg acgcggctct
    41341 tcttcacaaa aaagaacaac tctacaaaca agaattggcc tggatgcgca gacaaccgca
    41401 ggcgcgtgcg accaagcagc aggcccgtat caatcgattc catgatctga aaaaggaagt
    41461 ttcaggcagt agtgctgaga cagacttgac catgaacttt gaaaccagtc ggattgggaa
    41521 gaaagtcatc gagtttcagg ctgtttcctt tgcctatgaa aacaagccta ttttgcaaga
    41581 ttttaatctc ttggtgcagg ccaaagaccg tatcggaatc gttggggaca atggtgttgg
    41641 aaaatcaacc ctgctcaact tgattgcagg aagtcttgag ccaactaaag gacaagttgt
    41701 gattggggaa actgttcgca tcgcctattt ctctcagcaa attgagggtt tggatgaaag
    41761 caagagagtt atcaattacc tgcaggaagt ggcagaagag gtcaagacca gtggcggttc
    41821 tacgacttcg attgcagagt tgctggagca gttccttttc ccacgttcga cgcatggaac
    41881 cttgattgag aaattgtcag ggggtgagaa aaaacgtctt tatctcctca aactgctttt
    41941 ggaaaaacca aatgttcttc ttttagacga gccaaccaat gacctagata ttgcaacttt
    42001 gacagtctta gagaatttct tgcaaggttt tgcaggtccc gttttaacag tcagtcacga
    42061 ccgctatttc ttggataagg tagcgaccaa gattctcgct tttgaggatg gcaagattcg
    42121 tcctttcttt ggtcattaca ccgactatct tgatgaaaaa gcttttgaaa cagatatggc
    42181 caatcaagtg caaaaggccg aaaaggaaaa agtggtcaag gttcgagaag acaagaaacg
    42241 catgacctac caagaaaagc aggagtgggc aagtattgaa ggtgatattg aagccttgga
    42301 aaaacgtatc gctgctattg aagaggaaat gcaggctaac ggctctgact ttggtaagct
    42361 ggctactctc caaaaagaat tggatgagaa aaatgaagca ctccttgaaa aatacgaacg
    42421 ctatgagtat ctcagtgaat ttgatagttg atactattcg aaaatcaaag tgcaaactat
    42481 gtcggcttca cattgccgac tcaagtacag cctgtgaccg gcttcctaat ttgcactttg
    42541 atttttattg agtataaacg aatagaaaaa tcccgtctca tgaaaggatt tctctattct
    42601 tttttggttt cttgatgaaa gatattttgc caggtttcgt ggcgacgcca gatactggta
    42661 tggatgatac catctaactc atagcagata agtttggttt tttgactgat ggaagtaatt
    42721 tgaatgtcct tgatagcaga atttaactct tttttggtct tataagcctc tttatccatc
    42781 tgctctccat cttggcgaat atagacaaaa tcatcagtca agagttcttc tagttggttt
    42841 ccttggtcga tcaattgttc acgcataagc aattttttat agacattgtc taaaatttca
    42901 tcctcaggga taggtgctgg ttcgatatgg acatcggtat caaagacgcc aaaacgattc
    42961 tccagcatag actcgacctg atccgcgatt tcatggcttt caaaaacaga caagtcagga
    43021 ttcatctcta gtgtaatatc caggtagatg ttgctaccgt aggtgcgacc tctttgcgat
    43081 ttgaccttgc tgattttggg aatttccatg atagcctttt ggtagtcctc gagcaggcgg
    43141 tcgtcaaagc catctgaaag actaaaggaa gactcgatga agatatcata ggcagtcttc
    43201 aagataaaga aagtgatgat gatagcaacc agtttatcca caatcggata attgaaacta
    43261 ctagctagga tggcaatggc ggttccaagt gaggtaacag cgtcagaaag attgtcctta
    43321 gcagctgcct tcagcgcctt ggagttggat ttcttactga ggcgagtatt gtagagatag
    43381 accacaaaca taatcgctgc agaaatgatt cctagagttg caccaagagg atcaatgacc
    43441 gtttcttccc gactgagaat cttttgaatg gtatctctta gaacatcgaa accgacatag
    43501 aacatgatga tagaagtgat caagcttgcc aaatcttcaa tcttccaatg accaaaacgg
    43561 tggtcacggt ctgcaggctg gcgcgccatc cgaatcccga ttaagagggc cacatttcca
    43621 atgatgtccg atacgttatt aaaaccatcg gccaccaaac tggatgaatg aaggagatga
    43681 ccagctgcta atttggctgc agacaagatc aaataggtcg aaatactgat aatggctcca
    43741 cgctcagcta acttgagatt tgagatagat tgcttcattc aacttccttt ctagtcacat
    43801 agcattctac cattatactg gttttcaagg aaaaattcaa ataaggcagt ttttactctt
    43861 tgaaaactcc ccaaaatttg gtataatggt actactcaag ggagtagctg gcagaaacct
    43921 gtgatagtgt cgtcattccg aattttatgc tgaaaagtat gctttccggc cctatcttaa
    43981 acagcgagac ttgttatgat taacaggtct ctttttgttt gtcgtgaaaa gatatgacag
    44041 aaagagagtc tgttttgcac acaatgtcaa ggaggagaca catgtcaaaa gaacaaaaac
    44101 gccaagcgtt ttatactcag agccctgaag aggtcttgca ggctgtggat gcgaccgagc
    44161 aaggtttgtc atcaagtgag gcggaaaagc gccttgccga atttgggcac aatgaactcg
    44221 aagaaggcga gaaacgatca atcttggtca aattcatcga gcaatttaag gatttgatga
    44281 ttatcatcct agttgcggca gcaatcttgt cagtcgtgac ttctggtggg gaagatatcg
    44341 cagatgccat tatcatccta gctgtggtta tcatcaacgc tgcctttggt gtttaccaag
    44401 aaggaaaagc tgaagaagct attgaagccc tcaaatccat gtctagtcca gttgcccgcg
    44461 ttcttcgtga tggacacatg gcagagattg actctaaaga attggtacca ggcgatatcg
    44521 ttgcccttga agcaggtgac gtggtaccag cggacctacg tttgatagaa gccaactctc
    44581 ttaaaattga agaagcagcc ttgacaggtg aatctgtacc agtcgaaaaa gacttgtcag
    44641 tcgagcttgc gacagatgct ggtattggtg accgtgtcaa catggccttc caaaactcaa
    44701 acgtaaccta tggtcgtggg atgggtgttg ttgtcaatac aggtatgtac actgaagttg
    44761 gtcatattgc tggtatgctt caagatgcgg atgagactga tacaccactc aaacaaaatt
    44821 tgaacaacct ttctaaggtc ttgacctatg ctatcttggt cattgccctt gttacttttg
    44881 tagtgggtgt cttcattcaa gggaaaaatc cacttggtga gttgttgact tctgttgccc
    44941 ttgccgttgc agccattcca gaaggacttc ctgctatcgt taccatcgtt ctttctcttg
    45001 gtactcaagt tttggccaaa cgacattcca tcgttcgtaa gttgccagca gttgaaacac
    45061 ttggttcaac tgaaatcatc gcttctgata agactggtac gctgactatg aacaagatga
    45121 cagtcgaaaa agtcttctac gatgcggttc tacatgactc agctgatgat attgaactag
    45181 gtcttgaaat gccactactt cgttcagttg tcttggccaa tgatacgaaa atcgatgtgg
    45241 aaggtaactt gattggtgac ccaaccgaaa cagcctttat ccaatatgcc ttggacaagg
    45301 gctatgatgt caaaggtttc ttagagaaat atcctcgtgt agctgaattg ccatttgact
    45361 ctgaccgtaa gctcatgtca acagttcacc cattgccaga tggtcgtttc cttgtagcag
    45421 tcaagggtgc gccagaccaa ctcttaaaac gttgtcttct tcgtgataag gctggggata
    45481 ttactccgat tgatgagaag gttacaaatc tcattcatac aaacaattct gaaatggctc
    45541 atcaagcctt gcgtgtcctt gcaggtgctt ataagattat cgatagtatt ccagaaaatc
    45601 tcacttctga agagcttgaa aatgatttaa tttttactgg tttgattggg atgattgacc
    45661 ctgaacgtcc tgaagccgct gaggctgttc gtgtggctaa ggaagcggga atccgtccaa
    45721 ttatgattac aggtgaccac caagacacag cggaagccat tgccaaacgt ttgggaatca
    45781 ttgacgcaaa cgatacagaa ggtcacgttt taactggtgc tgaactcaat gaactgtcag
    45841 atgaagaatt tgaaaaagtc gttggtcaat actctgttta tgcccgtgtg tctccagaac
    45901 acaaggttcg tatcgtcaag gcttggcaaa aacaaggtaa agtcgttgcc atgacaggtg
    45961 acggtgtcaa tgacgcgcca gctctgaaaa cagccgatat cggtatcggt atgggaatca
    46021 ctggtacaga ggtttctaag ggggcttctg atatgattct tgcagatgat aactttgcga
    46081 ctattatcgt cgcagtggaa gaaggacgta aggtcttctc aaacattcaa aagactattc
    46141 agtacctact ttctgctaat actgctgaag tattaaccat cttcctatca accttgtttg
    46201 gttgggatgt cttacagccg gttcatcttt tgtggatcaa cttggtaacg gataccttcc
    46261 cagctatcgc tcttggtgtt gaacctgcgg aacctggtgt catgaatcat aaaccacgtg
    46321 gacgcaaggc aagcttcttc tcaggtggtg ttttgagttc tatcatttat caaggtgtac
    46381 tccaagcagc tcttgttatg agtgtttatg gccttgcgat tgcttaccca gttcatgtgg
    46441 gtgacaatca tgctattcat gcagatgccc taacgatggc ctttgcaacc cttggtttga
    46501 ttcagctctt ccatgcctac aatgtcaagt ctgtctacca atccatcttg acagttggcc
    46561 cattcaagtc taagaccttt aactggtcca tcttggtatc ctttatcctt ctcatggcaa
    46621 caatcgttgt agaaccgctt gaaggaatct tccacgtaac caaactagac ttgtcacaat
    46681 ggggaattgt tatggctgga agcttctcaa tgattatcat cgtcgaaatc gttaagttta
    46741 ttcaacgcaa actcggtttt gacaagaatg cgatttaagg gaggtttcgg atgtcagcat
    46801 atcaattacc gaccgtatgg caggatgaag ctagtaatca aggagctttt acggggctaa
    46861 acagaccaac agcaggtgcc cgtttcgaac aaaacttgcc aaaaggagaa caagcttttc
    46921 agctttattc actgggaaca ccaaatggtg tgaaggttac tatcttattg gaagaattac
    46981 tagaagctgg ttttaaggaa gcggcttacg acttgtataa gattgctatc atggatgggg
    47041 atcaattcgg ctcagacttt gtgaagctca atccaaattc caagattcca gccttattgg
    47101 accagtcagg tactgaaaac gtaagagtct ttgagtctgc tcatattctt ctttaccttg
    47161 ctgagaaatt tggagccttt ttaccaagta atcctgtgga aaaggtagaa gttttgaatt
    47221 ggctattctg gcaagcaggt gcagcacctt ttctaggtgg gggatttgga catttcttca
    47281 attatgctcc tgaaaaattg gaatatccta ttaaccgttt tacgatggaa gtgaaacgcc
    47341 agttggattt attggataag gaattggctc agaaacctta tattgcaggc aatgactata
    47401 cgattgcaga tattgctatc tggtcttggt atggacagtt agttcaagga aatctttacc
    47461 aaggttctgc aaaattcttg gatgcctcaa gttatcaaaa tctagtaaaa tgggcagaaa
    47521 aaattgccaa tcgtccagct gttaagcgtg gcttggaagt aacttataca gaaattaaat
    47581 agcaaaaaag tcatcttcgg atggcttttt gctacagttg agggaaaggg tttgcagtta
    47641 tcggcttttg tgctataatt gctataatat agtaaaaagc aagaggagtg tgaggaagtg
    47701 gaaaagaaaa ttgtgaagga tatcttattt ttatctcaag tgtctcagcc ggcaagtcag
    47761 gaggaccttt atcttgccag agatttgcag gatacactct tagcaaatcg tgatacctgt
    47821 gttggtctag ctgccaatat gattggggtg cagaagcgcg tgattatctt taatcttggc
    47881 ttggttcccg tggtcatgtt taacccagtg cttctgtcct ttgaaggatc ctatgaggca
    47941 gaagaaggct gtttgtcctt ggtaggtgtg agatcaacta agcgttatga aaccataagg
    48001 gttgcctatc gtgacagcaa gtggcaggaa cagaccatta ccttgacagg cttcccagct
    48061 cagatttgcc agcatgagct ggatcacttg gaaggacgaa tcatttagga ggaaagcaaa
    48121 tgaaacgaat agtctttgaa cttattttta tcgcaacgac ctggtatatc tttttaccgc
    48181 cccttaacct gaccagctgg gaatttctct tcttcctctg tgggcatttg ttagttgtgg
    48241 caatcttatt tggctttggc aaggggataa accttgtcaa aacggttcat gtgcgccacg
    48301 gtaaggcgga agctgcctta aatcttgagg gtttcaaaat caatcggtta gggaaaattc
    48361 tgttagcttc gattggagga attcttctct tggcagcttt ggtttccttg gtaacttcca
    48421 gcatgtttca ggctaaaaat tatgccaatg tagtcacggt tacggaaaaa gactttactg
    48481 aatttcctaa gagtgacacc agtaaggttc ctatcctaga tagaagtact gctgaaaaaa
    48541 ttggagaccg ctacttgggt tccctaaccg ataaggtgtc gcaatacgta gcggcagata
    48601 cctataccca attgacaatt gatgggaaac cttatcgggt cacaccacta gaatatgcag
    48661 accctatcaa atggtttaac aatcaagcca agggaatcgg tgagtatatc aaggtagaca
    48721 tggtaactgg aaatgcggat ttggtggact tgaagacacc aatcaagtat tcagactcgg
    48781 agtattttaa ccgtgatgtc aaacgtcacc tgcgcttgaa gtacccgacc aaaatcttta
    48841 aaactccatc ttttgaggtg aacgatgagg gcaatccttt ctatgtagca acggtttacc
    48901 aaaagcaatt tggacttgcg gttcctcgtc ctgcttcagt cattatcttg gatgctacaa
    48961 atggagaaac caaggaatac agcttatcag atgttccaga atgggtggac aggatctatc
    49021 cagcagagga aaccattgag caaatcaact acaacggcaa gtacaaggac ggtttcttga
    49081 atgccatgat ttccaagaaa aacgtgaccc agactaccaa tggctataat tacttgtcta
    49141 tcggtaatga catctatctc tacacaggtg tgacgtcggc taatgcggat gagagtaatc
    49201 ttggtttcat ccttgaaaat atgcgaacag gagaaattac taagtatagc ttggcttctg
    49261 cgacagaaga atcagcccgt gaatcagcag aaggtgctgt tcaggagaaa tcctacaaag
    49321 caaccttccc aatcctcatc aacctcaatg acaagcctct ctacatcatg ggcttaaagg
    49381 acaatgctgg cttggtcaaa gagtacgccc tggtagacgc agtcgagtac caaaatgtta
    49441 tcgttgctac tacagtggaa gagatgctca gcaagtatgc caataaaaac gaccttgaaa
    49501 ttgacaatgc aacgacagaa agcatcaagg gagtagtagc agacctcaaa tcagctgtta
    49561 tcaagggaga cactgtctac ttctttaaag ttgatggcaa gatatacaag gtaaaggctt
    49621 cagtatccga tgaccttcct taccttgaaa atggtaaaac cttcgaaggt caagtaggaa
    49681 aagacaatta tctcaagacc tttaaagtac aataatactc ttcgaaaatc tctagctcta
    49741 tctgcaacct caaaacagtg ttttgag
//
LOCUS       DBERDK010000006        48800 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.006, whole genome shotgun sequence.
ACCESSION   DBERDK010000006 DBERDK010000000
VERSION     DBERDK010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 48800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48800
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.006"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            266..409
                     /locus_tag="ACL73L_000318"
     CDS             266..409
                     /locus_tag="ACL73L_000318"
                     /inference="COORDINATES: protein motif:HMM:NF016232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGS0771368.1"
                     /translation="MDLKFEGVDLEYKKAKNNLPESFWETYSAFANTNGGKIILGIDE
                     KKH"
     gene            393..1248
                     /locus_tag="ACL73L_000319"
     CDS             join(393..812,814..1248)
                     /locus_tag="ACL73L_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HGS0771369.1"
                     /translation="MKKNIDPYQGVNRLPAKQNYEASKQLTDARFKRLVGVQRTTFEE
                     MLAVLKTAYQLKHAKGGRKPKLSLEDLLMATLQYVREYRTYEEIATDFGIHESNLIRR
                     SQWVEVTLVQSGVTISRTPLSSEDTVMIDATEVQINRPKKELANDSGKKKCHAMKAQA
                     IVTSQGRIVSLDITVNYCHDMKLFKMSCRNIGQAGKILADSGYQGLMKICPQAQTSRK
                     SSKLKPLTAEDKAYNHALSKERSKVENIFAKVKTFKMFSTTYRNHRKRFGLRMNLIAG
                     IINHELGF"
     gene            complement(1327..1584)
                     /locus_tag="ACL73L_000320"
     CDS             complement(1327..1584)
                     /locus_tag="ACL73L_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771370.1"
                     /translation="MLPTYTDSYFLSFVSDDVEERYTENVYLDSLSVKQKFKFIFDFG
                     DEWRFECQVLREIETEDEEAYLVRSVGTSPEQYPDYDGFDY"
     gene            complement(1943..3823)
                     /locus_tag="ACL73L_000321"
     CDS             complement(1943..3823)
                     /locus_tag="ACL73L_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HGS0771371.1"
                     /translation="MVRFTGLSSKQAQAIEVLKNHISLPDVEVAVTQSDQASISIKGE
                     GGHYQLTYRKPHQLYRALSLLVTVLAEADKVEIEEQAAYEDLAYMVDCSRNAVLNVAS
                     AKQMIEVLALMGYSTFELYMEDTYQIEGQPYFGYFRGAYSAEELQEIEAYAQQFDMTF
                     VPCIQTLAHLSAFVKWGVKEVQELRDVEDILLIGEEKVYDLIDGMFATLSKLKTRKVN
                     IGMDEAHLVGLGRYLILNGVVDRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDRVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPHNHFSRLVAIEANKACRANQIKEVIVTGWGDNGGETAQFSILPSLQIW
                     AELSYRNDLDGLSAHFKTNTGLTVEDFMQIDLANLLPDLPGNLSGINPNRYVFYQDIL
                     CPILDQHMTPEQDKPHFAQAAETLANIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RQAYQADDKESLQQIARQELPELRSQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEVYLAGQLDRIDELEVEILPFTDFYADKDFAATTANQWHTIATASTIY
                     TT"
     gene            complement(3817..4686)
                     /locus_tag="ACL73L_000322"
     CDS             complement(3817..4686)
                     /locus_tag="ACL73L_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HGS0771372.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILNKTSIPTPESLEDLLAWLD
                     QRLSEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIHGFSWYEALSSYQIPVHLENDAN
                     CVGLSELLAHPELENAACVVIGTGIGGAMIINGRLHRGRHGLGGEFGYMTTLAPAEKL
                     NNWSQLASTGNMVRYVIEKSGQTDWDGRKIYQEAAAGNAICQEAIERMNRNLAQGLLN
                     IQYLIDPDVISLGGSISQNPDFIQGVKKAVEDFVDTYEEYTVAPVIQACTYHADANLY
                     GALVNWLQEENQW"
     gene            complement(4770..7415)
                     /locus_tag="ACL73L_000323"
     CDS             complement(4770..7415)
                     /locus_tag="ACL73L_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HGS0771373.1"
                     /translation="MENVVVHIISHSHWDREWYLPFESHRMQLVELFDNLFDLFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQQEAAKWGKSTQIGYFPDTFGNMGQAPQILQKSGIHVAAFGRGVKPIGFDNQV
                     LEDEQFTSQFSEMYWQGVDGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNQWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHSSFDEYVQAVEGALPEHL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVEPLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVSTVIDVATCDFKELHPTEGYKKMAALILPSYRVEDL
                     DGRPVEATIEDLGANFEYDLPKDKFRQARIARQVRVTILVHLAPLSWTTFQLLEGKQE
                     HREGIYQNGVIDTPFVTVSVDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKGHEVLENTDRYAKILLKHELTVPVSADEKLEEEQQGIIEFMKREAGRSE
                     ELTNIPLETELTVFVDNPQIRFKTRFTNTAKDHRIRLLVKTHNTRPSNDSESIYEVVT
                     RPNKPAASWENPENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILGDNTIAVTILRASG
                     ELGDWGYFPTPEAQCLREFEVEFAIECHQAQERFSAYRRAKALQTPFTSLQLARQEGS
                     VVATGSLLSHSVLSIPQVCPTAFKVAENEEGYVLRYYNMSQENVRISEHQQTILDLLE
                     RPYPVHSGLLAPQEIRTELIKKEEI"
     gene            complement(7506..8786)
                     /locus_tag="ACL73L_000324"
     CDS             complement(7506..8786)
                     /locus_tag="ACL73L_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HGS0771374.1"
                     /translation="MIYSKEIVREWLDEVAERAKDHPEWVDVFERCYTDTLDNTVEIL
                     EDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDALLRQTIAGLVKRQMTLVLKDPYA
                     NSFNIEENWKGHHETDHTDLNGWIWERKYEVDSLCYPLQLAYLLWKETGETSQFDETF
                     VAATKEILHLWTVEQDHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSD
                     DCCQYSYLIPSNMFAVVVLGYVQEIFAALNLADSQSVIADAKRLQDEIQEGIKNYAYT
                     TNSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCSVDDEVYQATRRTILSSEN
                     PYFYQGEYASGLGSSHTFYRYIWPIALSIQGLTTRDKAEKKFLLDQLVACDGGTGVMH
                     ESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR"
     gene            8957..11041
                     /locus_tag="ACL73L_000325"
     CDS             8957..11041
                     /locus_tag="ACL73L_000325"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HGS0771375.1"
                     /translation="MKPLLETIDTRFGTASKHAFSRGNTLPYTGVPFGMNYFVPQTSD
                     QGGSWFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTSQLGGDSLFHRQSSYDIDK
                     ACFQPHYLKLFSLRYQIETQLTPTCYGASIRLKQKQGKALSLYLHAADELTVEQVDKR
                     TLALRQEGKTETNKNSLTMFTALQMNTDILAISQEGGDWRIDLASSQTEMQLATSFIS
                     PSQALLNLPQEDFDSCKSSAQADWENLLHRFDIIEIGEADRTFFDHCLYRLFLFPQTF
                     YEINESGQAIHMDLATGTVKPGVLFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTGFLPKWLAPDERGMMPGTLLDGIIADSACKDMAPDLEGELFQAMLETASKADP
                     LGINGRHGLAQYQELGYLSTDHHESVSHTLDYAYSDFCIANCAKKLKNIEIAETYKAA
                     SQNYRQLFDAETGYMRARDNQGNFHPDFSPYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKACQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFHPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGIPLFDHLRVYLAKEDKWLDIHTKQNHNHFNFVKECRLDKTLVSTIQHQDLLKAE
                     QLTFTLSWLPSH"
     gene            complement(11081..12760)
                     /locus_tag="ACL73L_000326"
     CDS             complement(11081..12760)
                     /locus_tag="ACL73L_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HGS0771376.1"
                     /translation="MKKIKPHGPLPSQTQLAYLGDELAAFIHFGPNTFYDQEWGTGQE
                     DPERFNPSQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSVKVSPWRRGK
                     GDLLLEVSQAATEFDMDMGVYLSPWDAHSPLYHVDREADYNAYYLAQLKEILSNPNYG
                     NAGKFAEVWMDGARGEGAQKVNYEFEKWFETIRDLQGDCLIFSTEGTSIRWIGNERGY
                     AGDPLWQKVNPDKLGTEAELNYLQYGDPSGTIFSIGEADVSIRPGWFYHEDQDPKSLE
                     ELVEIYFHSVGRGTPLLLNIPPNQAGLFDAKDIERLYEFATYRNELYKEDLALGAEVS
                     GPALSADFACRHLTDGLETSSWANDADLPIQLELDLGSPKTFDVIELREDLKLGQRIS
                     AFHVQVEVDGVWQEFGRGFTVGHKRLLRGPLVEAQKVRVMITEAQSMPLLTKISLYKT
                     PGLSKKEVVQELAFAEKSLAVAKGETLHFRIERSESSSSLEAKISIQPGTGVHGVAYQ
                     DEIQVLQFQAGESKKDLHLPTLYFAGDKTLDFYLNLTVDGQLVDQLQVQVS"
     gene            13516..14745
                     /gene="arcA"
                     /locus_tag="ACL73L_000327"
     CDS             13516..14745
                     /gene="arcA"
                     /locus_tag="ACL73L_000327"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HGS0771377.1"
                     /translation="MSSHPIQVFSEIGKLKKVMLHRPGKELENLLPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGIEVLYLEQLAAESLTSPEIRDQFIEEYLDEANIRDRQT
                     KVAIRELLHGIKDNQELVEKTMAGIQKVELPEIPDEAKDLTDLVESDYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMFADTRNRETLYGKYIFKYHPIYGGKVDLVYNREEDTRIE
                     GGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGDLHVYSVTYENEKLKIVEEKGDLAELLAQNLGVEKVHLIR
                     CGGGNIVAAAREQWNDGSNTLTIAPGVVVVYDRNTVTNKILEEYGLRLIKIRGSELVR
                     GRGGPRCMSMPFEREEV"
     gene            14803..15819
                     /gene="argF"
                     /locus_tag="ACL73L_000328"
     CDS             14803..15819
                     /gene="argF"
                     /locus_tag="ACL73L_000328"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HGS0771378.1"
                     /translation="MTNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHY
                     LAGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLV
                     YCGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITED
                     ADEAVKDADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPA
                     FHDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLY
                     IPKV"
     gene            15984..16931
                     /gene="arcC"
                     /locus_tag="ACL73L_000329"
     CDS             15984..16931
                     /gene="arcC"
                     /locus_tag="ACL73L_000329"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HGS0771379.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQQEALVETAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDE
                     GIEKNVASVVTQVVVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWR
                     KVVASPKPVDIKEIETIRTLLNNGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFAS
                     QRLAELVDADLFIILTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPK
                     VEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG"
     gene            17142..18653
                     /locus_tag="ACL73L_000330"
     CDS             17142..18653
                     /locus_tag="ACL73L_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HGS0771380.1"
                     /translation="MSEKAKKGFKMPSSYTVLLIIIAIMAVLTWFIPAGAFIEGIYET
                     QPQNPQGIWDVLMAPIRAMLGTHPEEGSLIKETSAAIDVAFFILMVGGFLGIVNKTGA
                     LDVGIASIVKKYKGREKMLILVLMPLFALGGTTYGMGEETMAFYPLLVPVMMAVGFDS
                     LTGVAIILLGSQIGCLASTLNPFATGIASATAGVGTGDGIVLRLIFWVTLTALSTWFV
                     YRYADKIQKDPTKSLVYSTRKEDLKHFNVEESSSVESTLSSKQKSVLFLFVLTFILMV
                     LSFIPWTDLGVTIFDDFNTWLTGLPVIGNIVGSSTSALGTWYFPEGAMLFAFMGILIG
                     VIYGLKEDKIISSFMNGAADLLSVALIVAIARGIQVIMNDGMITDTILNWGKEGLSGL
                     SSQVFIVVTYIFYLPMSFLIPSSSGLASATMGIMAPLGEFVNVRPSLIITAYQSASGV
                     LNLIAPTSGIVMGALALGRINIGTWWKFMGKLVVAIIVVTIALLLLGTFLPFL"
     gene            18675..20006
                     /locus_tag="ACL73L_000331"
     CDS             18675..20006
                     /locus_tag="ACL73L_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HGS0771381.1"
                     /translation="MKIDITNQVKDEFLISLKTLISYPSVLNEGENGTPFGQAIQDVL
                     EKTLEICRDIGFTTYLDPKGYYGYAEIGQGAELLAILCHLDVVPSGDEADWQTPPFEA
                     TIKDGWVFGRGVQDDKGPSLAALYAVKSLLDQGIQFKKRVRFIFGTDEETLWRCMARY
                     NTIEEQASMGFAPDSSFPLTYAEKGLLQVKLHGPGSDQLELEVGGAFNVVPDKANYQG
                     PLYEQVCNGLKEAGYDYQSTEQTVTVLGVPKHAKDASQGINAVIRLATILAPLQEHPA
                     LSFLATQAGQDGTGRQIFGDIADEPSGHLSFNVAGLMINHERSEIRIDIRTPVLADKE
                     ELVELLTRCAQNYQLRYEEFDYLAPLYVAKDSKLVSTLMQIYQEKTGDNSPAISSGGA
                     TFARTMPNCVAFGALFPGAKQTEHQANECAVLEDLYRAMDIYAEAVYRLAT"
     gene            20101..20208
                     /locus_tag="ACL73L_000332"
                     /pseudo
     CDS             20101..20208
                     /locus_tag="ACL73L_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(20412..20702)
                     /locus_tag="ACL73L_000333"
     CDS             complement(20412..20702)
                     /locus_tag="ACL73L_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZmpA/ZmpB/ZmpC family metallo-endopeptidase"
                     /protein_id="HGS0771382.1"
                     /translation="MGALGYYEGFVPYVSNQYKNQAEEEYKPLSDKYIFEKILGKTYA
                     AFKKDQINERVEKLGKLKPITINYNGKSEVIDSKEKLQELMNKAVKDEVAQI"
     gene            complement(20690..20941)
                     /locus_tag="ACL73L_000334"
     CDS             complement(20690..20941)
                     /locus_tag="ACL73L_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657761.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HGS0771383.1"
                     /translation="MKEFQFERKQRFSLRKYAIGACSVLLGTSLFFAGMGAQPVQDTE
                     TSSALISSHYLDEQDLSEKLKSELQWFELENKLLNLWEH"
     gene            21145..21303
                     /locus_tag="ACL73L_000335"
     CDS             21145..21303
                     /locus_tag="ACL73L_000335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771384.1"
                     /translation="MKIKEQTRKLATGCSKHRFEVVDKTDEVSSKHCFEVADRTDEVS
                     NIYTARRR"
     gene            complement(21340..22224)
                     /locus_tag="ACL73L_000336"
     CDS             complement(21340..22224)
                     /locus_tag="ACL73L_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HGS0771385.1"
                     /translation="MIFLIVCVLLLVIVTLSTVYVVRQQSVAIIERFGKYQKVANSGI
                     HIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTDAYYKL
                     IRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYIIVKTLI
                     TKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRLHGVGIAQQ
                     RKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPNTPN
                     GVDDIRTQILSALRAEKK"
     gene            complement(22369..23520)
                     /locus_tag="ACL73L_000337"
     CDS             complement(22369..23520)
                     /locus_tag="ACL73L_000337"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HGS0771386.1"
                     /translation="MATFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKYIEGSD
                     ILPKTLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHGCDFIISLGGGSPQDAA
                     SCISIMATNGGKPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMLSKPKGLTAATGMDALTHAVEALVTPGAYDVTKKLSIGAI
                     ELIKEYLPRAVENGHDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIAEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF"
     gene            complement(23666..25432)
                     /locus_tag="ACL73L_000338"
     CDS             complement(23666..25432)
                     /locus_tag="ACL73L_000338"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HGS0771387.1"
                     /translation="MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLK
                     YPDGEPVECVISPSTIGRVPEAAASHELFKKSNVCATITVTPCWCYGSETMDMSPDIP
                     HAIWGFNGTERPGAVYLAAVLASHAQKGIPAFGIYGRDVQEASDTDIPEDVKEKLLRY
                     ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRS
                     IYDPEEFERALKWVKENVKEGFDHNREDLVLSREEKDRQWEFVIKMFMIGRDLMVGNP
                     RLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFAT
                     ENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVKRVTGHTLEGRAAAGFLHLINSG
                     SCTLDGTGQATRDGKPVMKPFWELEESEVQAMLENTDFPPANREYFRGGGFSTRFLTK
                     GDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTDPGWPTTWFAPRLTGK
                     GAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFRPKNWSL
                     FGTEDLESADYCACQLLGPLHK"
     gene            complement(25515..28631)
                     /locus_tag="ACL73L_000339"
     CDS             complement(25515..28631)
                     /locus_tag="ACL73L_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 98 C-terminal
                     domain-containing protein"
                     /protein_id="HGS0771388.1"
                     /translation="MNKEKIKRKLITILFVCIGMLCFGLLAGVKADNRVQMRTTINNE
                     SPLLLSPLYGNDNGNGLWWGNTLKGAWEAIPEDVKPYAAIELHPAKVCKPTSCIPRDT
                     KELREWYVKMLEEAQSLNIPVFLVIMSAGERNTVPPEWLDEQFQKYSVLKGVLNIENY
                     WIYNNQLAPHSAKYLEVCAKYGAHFIWHDHEKWFWETIMNDPTFFEASQKYHKNLVLA
                     TKNTPIRDDAGTDSIVSGFWLSGLCDNWGSSTDTWKWWEKHYTNTFETGRARDMRSYA
                     SEPESMIAMEMMNVYTGGGTVYNFECAAYTFMTNDVPTPAFTKGIIPFFRHAIQNPAP
                     SKEEVVNRTKAVFWNGEGRISSLNGFYQGLYSNDETMPLYNNGRYHILPVIHEKIDKE
                     KISSIFPNAKILTKNSEELSSKVNYLNSLYPKLYEGDGYAQRVGNSWYIYNSNANINK
                     NQQVMLPMYTNNTKSLSLDLTPHTYAVVKENPNNLHILLNNYRTDKTAMWALSGNFDA
                     SKSWKKEELELANWISKNYSINPVDNDFRTTTLTLKGHTGHKPQINISGDKNHYTYTE
                     NWDENTHVYTITVNHNGMVEMSINTEGTGPVSFPTPDKFNDGNLNIAYAKPTTQSSVD
                     YNGDPNRAVDGNRNGNFNSGSVTHTRADNPSWWEVDLKKMDKVGLVKIYNRTDAETQR
                     LSNFDVILYDNNRNEVAKKHVNNLSGESVSLDFKEKGARYIKVKLLTSGVPLSLAEVE
                     VFRESDGKQSEEDIDKITEDKVVSTNKVATQSSTNYEGVAALAVDGNKDGDYGHHSVT
                     HTKADSNAWWQVDLGEEFTVSKVDIYNRTDAEPQRLSNFDVIFLSSSGEEVFRRHFDK
                     VVDGLLSLKVPSVGAKLVKIELKSAAIPLSLAEVEVYGSKRTPKKLSNIALTKETRQS
                     STDYNGFSRLAVDGNKNGDYGHHSVTHTKEDSPSWWEIDLAQTEELEKLIIYNRTDAE
                     IQRLSNFDIIIYDSNDYEVFTQHIDSLESNNLSIDLKGLKGKKVRISLRSAGIPLSLA
                     EVEVYTYK"
     gene            complement(28641..30935)
                     /locus_tag="ACL73L_000340"
     CDS             complement(28641..30935)
                     /locus_tag="ACL73L_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HGS0771389.1"
                     /translation="MKLWYKKAASNWNEALPIGNGHLGGMIYGSATKECIQLNDETIW
                     YRGKSDRNNPDSLLHLKKIREYLLDGEIQKAEELIKLTMFATPRDQSHYELLGELYIE
                     HIDIQSCALSLYERELDLDTAISNVVFEPNSCNLQIKREYFTSFNKNILCCRIVSSVQ
                     NTLNLNINLGRNKRFNDEVSKLDSSTILMSASAGGRKGVQFKVVCHSKVTDGEVSVLG
                     ETIVIRNATEVFLYLKSMTDYWGNIDISSLQGEFSSIDYFTEKDEHVKKYQEQFNRVD
                     FKLDYSKDCLSIPTNLLLENTKKYSNYLTNLLFHYGRYLLISSSQPNGLPANLQGIWC
                     DELNPIWGSKYTININTQMNYWMVGPCDLPEVEYPLFDMLERMREPGRLTAKKMYGAR
                     GFTAHHNTDGFGDTAPQSHAMGAAIWVLTIPWLCTHIWEHYLYFQDERILTEHFEMIK
                     EAFLFFEDYLFEVDGYLMTGPSVSPENKYRLKNGIEGNACLSSTIDNQILRYFCDSCI
                     GIAKQLGDNSDFISRVKELKKKLPKTKIGSNGQIQEWLEDYEEVEPGHRHISPLFGLY
                     PYNEIDIHKTPELAEAAKITINRRLSNANFLSSQEREQAINNWLVSGLHASTQTGWSA
                     AWLIHFFARLYQGEPAYNQINGLLNNATLGNLFLDHPPFQIDGNLGLVSGICELLVQS
                     HHNWLSLIPALPSAWSEGEVKGFRVRGGYKVSFAWKNGDITFLKLEGGNKDQKVRVRI
                     YGKNTDVQNIELVFNSEKIIELNF"
     gene            complement(30994..31794)
                     /locus_tag="ACL73L_000341"
     CDS             complement(30994..31794)
                     /locus_tag="ACL73L_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HGS0771390.1"
                     /translation="MMNNKVTKVELKKVFKRSFMYGSSWNYERMQNLGFLYTILPVLK
                     KLYPDKDSASPAMKRHLEFFNTHQTAAPFILGVTSAMEEQEGNEGAASITGIKVGLMG
                     PLAGLGDSLFWLTLVPICFSIGASYSKDGGALGIFIALILFNIINIPVKYFGLKYGYT
                     KGSSLIQENNTKGTLNRVTSMATALGLVLVGGLIPSMVGINFGLEFKQGELVISVQEM
                     ITKLIPGFIPMALTLLMCKLIRKGKNPVVLIFSVMAIGVILVVLGILK"
     gene            complement(31791..32564)
                     /locus_tag="ACL73L_000342"
     CDS             complement(31791..32564)
                     /locus_tag="ACL73L_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HGS0771391.1"
                     /translation="MLFTQALLVTLVGIIATIDYNGPLFMIHRPLVTSAMVGLVLGDF
                     TQGVLIGSALELTWLGVTGIGGYTPPDTISGAIIGTAFGILSGQGETAGIAIAVPIAV
                     ATQQLDVLAKTLDVYFVKKADNDAKNGDYSKIGFYHYSSLVLITLFKIVPIFLAIMLG
                     GEYVADLFAKVPPIVMQGLNSAGALLPSIGFGMLLNMMLKKNMWVFLLIGFICSVYGG
                     MSTIGISLVGIAVAYFYDMIGSKPQETTSSSDVEEDLDL"
     gene            complement(32589..33059)
                     /locus_tag="ACL73L_000343"
     CDS             complement(32589..33059)
                     /locus_tag="ACL73L_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGS0771392.1"
                     /translation="MSIEFVRIDDRLVHGQVVTTWLKKYDIEQVIIVNDRISEDKTRQ
                     SILKISAPVGLKIVFFSVKRFVEVLNSVPIKKRTMLIYTNPKDVYDSIEGNLKLEYLN
                     VGQMSKTEENEKVTGGVALGEEDKYYFKKIVDKGTRVEIQMVPNDKVTMLEKFL"
     gene            complement(33050..33481)
                     /locus_tag="ACL73L_000344"
     CDS             complement(33050..33481)
                     /locus_tag="ACL73L_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGS0771393.1"
                     /translation="MFNRGILVASHGNFASGALMTAEMFVGETTNDRVRTLGLMPGEN
                     IVEFEHYFKNQVNELLDSNQEVIVLTDLIGGSPNNVALSRFLNLDSVDIVTGFNIPLL
                     VELISSYDSKINLEEIVQNAQNSLFNVKQQLNVEEEEDLCL"
     gene            complement(33474..33914)
                     /locus_tag="ACL73L_000345"
     CDS             complement(33474..33914)
                     /locus_tag="ACL73L_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HGS0771394.1"
                     /translation="MLKHIPKNISPDLLKTLMEMGHGDEIVLADANYPSASCANKLIR
                     CDGVNIPELLDSILYLMPLDSYVDSSIQFMNVVSGDDIPKIWGTYRQMIEGHGTDLKT
                     ITYLRREDFYERSKKAYAIVATGETSLYANIILKKGVVVERENV"
     gene            complement(33926..34564)
                     /locus_tag="ACL73L_000346"
     CDS             complement(33926..34564)
                     /locus_tag="ACL73L_000346"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HGS0771395.1"
                     /translation="MSDVKQELIKYGKKLVETDLTKGTGGNLSVFDREKQLMAITPSG
                     IDFFEIKESDIVVMDINGNVVEGERLPSSEWYMHLIQYQTRDDIDAIIHAHTTYATVL
                     ACLREPLPASHYMIAVAGKDVRVAEYATYGTKELAVNAAKAMEGRRAVLLANHGILAG
                     AQNLLNAFNIVEEVEYCAKIYCLAKNFGEPVVLPDEEMDLMAEKFKTYGQRK"
     gene            complement(34679..36082)
                     /locus_tag="ACL73L_000347"
     CDS             complement(34679..36082)
                     /locus_tag="ACL73L_000347"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase family protein"
                     /protein_id="HGS0771396.1"
                     /translation="MEAVLAIDLGATSGRAIVGYLSENKLVMEEINRFSNLPIRVKGH
                     LSWDVDFLLAKILESIRLANTSYKILSIGIDTWGVDFGLIDNEGKLLSQPVHYRDERT
                     KGVLKEISEMTELEKLYSETGNQIMEINTLFQLFKARQESPDSFYKTNKILLMPDLFN
                     YLLTGKFATEKSIASTTQLFDPRSQNWNQNILKLFELDSSLLPEIVSEGNVLGRIKKE
                     YGLGDIPVVNVCSHDTASAIVSVPKTEGSLFISSGTWSLVGVELTSPILTTESFSYGF
                     TNEVGKDGEITFLKNCTGLWIIEELRRSFERRGKAYSFDDIRTMVEKEKENLPLIDTE
                     STEFATESDMHKTLTEYLAYHHETREWTDGQLFKIVYESLAETYRKAIELLEELTHKV
                     YKRIYVIGGGARASYFNQMIADRTGKEVLTGSTEGTAVGNIVVQLIAMGQLKGMEEAH
                     HVIEEFLQLESYYSQKN"
     gene            36258..37031
                     /locus_tag="ACL73L_000348"
     CDS             36258..37031
                     /locus_tag="ACL73L_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGS0771397.1"
                     /translation="MIKDERVLELIEIIKKKKRIAVKELAEITFSSTSTLRRDLIFLE
                     NQGLIKRKHGYVTLSSMNTIELSHQIREGESTRQKRLIASLAKDFIRSGMCIYLDSST
                     TVYELCPYLSELDNLIIFTNGLHTAQTLSETVKDSSKIFITSGEVKHQSCSVVNYDKE
                     NSLLDHFNIDLAFCSARGIDDQYVYEASLSQAISKKNIIDKAHETILLIDSSKFYKTG
                     FFKINPLSKYTTFISDTLPNQKLLDAVELFDGEWVSDIQ"
     gene            complement(38191..39696)
                     /gene="adcA"
                     /locus_tag="ACL73L_000349"
     CDS             complement(38191..39696)
                     /gene="adcA"
                     /locus_tag="ACL73L_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HGS0771398.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSAKAVAKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIKATGDMLLLPGGEEEEGDHDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLS
                     ADYPDKKETFEKNAAAYIEKLQALDKAYAEGLSQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDAEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISVMEKNLKALKQTTDQEGPAIEPEKAEDTKTVQNGYFEDAA
                     VKDRTLSDYAGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYQTDVTK
                     INITDNTMEFVQGGQSKKYTYKYVGKKILTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNVAPVKAEHFHIFFGGTSQEALFEEMDNWPTYYPDNLSGQEIAQEMLAH"
     gene            complement(39706..40512)
                     /locus_tag="ACL73L_000350"
     CDS             complement(39706..40512)
                     /locus_tag="ACL73L_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HGS0771399.1"
                     /translation="MLSLLSYDFIQRAFLAVIAMSLFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGVAFGLVLGISPTVSTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGKSSSSMSLDQYLFGSIVTISEEQVISLFVIAAVVLILTFLFLRPMYILTFD
                     EDTAFVDGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFILISLVRRFIK"
     gene            complement(40505..41209)
                     /locus_tag="ACL73L_000351"
     CDS             complement(40505..41209)
                     /locus_tag="ACL73L_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HGS0771400.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYSVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVAISKTNTQGKKLRIAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RKGWFRRLNAHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHSAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENGQEVGHA"
     gene            complement(41209..41649)
                     /gene="adcR"
                     /locus_tag="ACL73L_000352"
     CDS             complement(41209..41649)
                     /gene="adcR"
                     /locus_tag="ACL73L_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HGS0771401.1"
                     /translation="MRQLAKDINAFLNEVILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELARRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQRFLTALVGEIK"
     gene            complement(41804..43072)
                     /gene="dltD"
                     /locus_tag="ACL73L_000353"
     CDS             complement(41804..43072)
                     /gene="dltD"
                     /locus_tag="ACL73L_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HGS0771402.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMHHNLGAEKRSAVAT
                     TIDSFKERSQKVRALSDPNVRFVPFFGSSEWLRFDGAHPAVLAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLPQLENKQVVYVISPQWFSKNGYDPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLVQKLASKEELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIEDVVKADAEKNTSNNEMGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFSKSKVNPIFIIPPVNKKWMDYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NPTPAPTYHLNERFFSKDWATYDGDVKEFQ"
     gene            complement(43065..43304)
                     /gene="dltC"
                     /locus_tag="ACL73L_000354"
     CDS             complement(43065..43304)
                     /gene="dltC"
                     /locus_tag="ACL73L_000354"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HGS0771403.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDIRVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            complement(43318..44562)
                     /gene="dltB"
                     /locus_tag="ACL73L_000355"
     CDS             complement(43318..44562)
                     /gene="dltB"
                     /locus_tag="ACL73L_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HGS0771404.1"
                     /translation="MMEFFQQLPHLEPYGNPQYFVYVIAATLPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILLLLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINGTQSLLGFLGISYLTFRSVGIIIELRDGVIKDFTLWEFLRFLLFM
                     PTFSSGPIDRFKRFNENYQAIPERDELMDMLDESVRYIMRGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALAISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLIMGF
                     WHGVTWYYIAYGLFHGLGLVINDAWVRKKKTLNKERKKAGKAALPENRWIQLLGMVVT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            complement(44559..46109)
                     /gene="dltA"
                     /locus_tag="ACL73L_000356"
     CDS             complement(44559..46109)
                     /gene="dltA"
                     /locus_tag="ACL73L_000356"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HGS0771405.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQEHTYGDLKADSDS
                     LAAVIDQLGLPEKSPVVVFGGQEYEMLATFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPSLIIAISAFPLEQVSTPMINLAQVQEAFAQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMITDKEFATPSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWTSTPSFADMAMLSEYFNSEKMPGITHFYFDG
                     EELTVKTAQKLRERFPNARIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMNNPEKTAEAFFEFEGLPAYHTGDVGT
                     MTDEGLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKDGVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            complement(46130..46261)
                     /locus_tag="ACL73L_000357"
     CDS             complement(46130..46261)
                     /locus_tag="ACL73L_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HGS0771406.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            complement(46575..47753)
                     /locus_tag="ACL73L_000358"
     CDS             complement(46575..47753)
                     /locus_tag="ACL73L_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ryptide export MFS transporter"
                     /protein_id="HGS0771407.1"
                     /translation="MSNSFVKLLVSQLFANLADIFFRVTIIANIYIISKSVIATSLVP
                     ILIGISSFVASLLVPLVTKRLALNRVLSLSQFGKTILLAILVGMFTVMQSVAPLVTYL
                     FVVAISILDGFAAPVSYAIVPRYATDLGKANSALSMTGEAVQLIGWGLGGLLFATIGL
                     LPTTFIILVLYIISSFLMLFLPNAEVEVLESETNLEILLKGWKLVARNPRLRLFVSAN
                     LLEIFSNTIWVSSIILVFVTELLNKTESYWGYSNTAYSIGIIISGLIAFRLSEKFLAA
                     KWESILFPLVAMAIVTLTILYFPNAQIFLLFSALVGMLSQLKEVPESVFLQETVEENH
                     LVNVYSVLEVISTLAFSVFVLLMSYIAESFGISISFWLSAICLMIEAILIYIRRDYFK
                     "
     gene            complement(48209..>48800)
                     /locus_tag="ACL73L_000359"
     CDS             complement(48209..>48800)
                     /locus_tag="ACL73L_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="group II intron maturase-specific
                     domain-containing protein"
                     /protein_id="HGS0771408.1"
                     /translation="NQTEAASKRVMYSVSRFIEKRLGLKVNMTKTKITRPRELKYLGF
                     GFWKSSDGWKSRPHQDSVRRFKFKLKKLTQRKWSIDLTRRIEQLNLSIRGWINYFSLG
                     NMKSIVASIDERLRTRLRVIIWKQWKKKSRRLWGLLKLGVPKWIADKVSGWGDHYQLV
                     AQKSVLKRAISKPVLEKRGLVSCLDYYLERHALKVS"
BASE COUNT        14602 a        10588 c         8820 g        14790 t
ORIGIN      
        1 agttttattc aatcattatc tagtagaata gagtagtaca ttttataaat tgtcatctct
       61 ctataaacac agtatatcat ttttaaattt taagtcaagg aaaattaacc cactttaatt
      121 tcttgtttaa cccgggttaa aaaagaaatt aaagtgggtt aaaaaagaaa ttaactgagt
      181 aaaaactctc gaaattcaat tcgctatatt atcactcaac ttgaacaaat accagaacca
      241 caaaaaacag atgtaaagga aaaatatgga cttaaaattt gaaggggttg atttagaata
      301 caaaaaagct aaaaacaatc ttccagagtc tttctgggaa acctactcag catttgcaaa
      361 tacaaatggc gggaaaataa ttctcgggat agatgaaaaa aaacattgac ccctaccaag
      421 gagtcaatag acttcctgcg aaacaaaatt atgaagcaag taaacaacta accgatgcac
      481 gatttaagcg tcttgttggt gttcagcgca ccacttttga agagatgtta gctgtgttaa
      541 aaacagctta tcaacttaaa cacgcaaaag gtggacgaaa acctaaatta agcctagaag
      601 accttcttat ggccactctt caatatgtgc gagaataccg cacttatgaa gaaattgcga
      661 ctgattttgg tattcacgaa agcaacttaa tccgtcggag ccaatgggtt gaagtaactc
      721 ttgttcaaag tggtgttacg atttcaagaa ctcctctcag ttctgaggac acggtaatga
      781 ttgatgcgac ggaagtacaa atcaatcgcc ctaaaaaaag aattagcgaa tgattctggt
      841 aaaaagaaat gccacgctat gaaggctcaa gcgattgtca caagtcaagg gagaattgtt
      901 tctttggata tcactgtgaa ctattgtcat gatatgaagt tgttcaaaat gagttgcaga
      961 aatatcggac aagctggtaa aatcttggct gacagtggtt atcaagggct catgaagata
     1021 tgtcctcaag cacaaacttc acgtaaatcc agcaaactca agccactaac agctgaagat
     1081 aaagcctata accatgcgct atctaaggag agaagcaagg ttgagaacat ctttgccaaa
     1141 gtaaaaacgt ttaaaatgtt ttcaacaacc tatcgaaatc atcgtaaacg cttcggatta
     1201 cgaatgaatt tgattgctgg tattatcaat catgaactag gattctagtt ttgcaggaag
     1261 tctattgaaa tactaagcct acacagactg attccaattg ttttttagat ttcaatttta
     1321 ccattcttaa tagtcaaaac catcataatc tggatattgt tctggcgacg ttccaaccga
     1381 acgtacgaga taagcttctt cgtcctctgt ctcgatttct ctcagcactt ggcattcaaa
     1441 acgccattca tcaccgaagt cgaaaataaa cttaaatttt tgtttgacac ttaggctatc
     1501 cagatagaca ttttctgtgt aacgttcttc aacatcgtca ctaacaaagc taagaaagta
     1561 agaatctgta tatgtcggta gcaacctttt ttaaatttga cggaaatgac ccaggcttct
     1621 acagatgctg acttttcagc ttctatttct tattttacta attttaagtg taccataata
     1681 cctccaagtg ctcatcgata gaattgctct taggaaacat tcactttctg ctattttatc
     1741 ataagtttgt ctaactgtag catttgaaac cagataattc ttctgtttca cgcgaaacac
     1801 cctgcgccaa gcaaggtgtt attgttttta tactcaatga aaatcaaaga gcaaactagg
     1861 aagctagctg caggctgctc aaaacactgt tttgaggttg tagatggaag ctgacgtggt
     1921 ttgaagagat tttcgaagag tattaagtcg tataaatcgt cgaagctgtc gcaatggtat
     1981 gccactggtt agctgtagtt gctgcgaaat ccttgtctgc gtagaagtca gtaaatggta
     2041 ggatttcaac ttccagctcg tcgatgcggt caagctggcc agccagataa acctcgatac
     2101 ggctttctgc tcgtttgatg cgttgcaaga gtccgcccat acggatgtca actgtatcca
     2161 aaccaaagac cttgttttct ttcagccatt ggtggctaaa gagggcatgg aagtcttcaa
     2221 tttggcttct aagttctggt aattcttgtc tggcgatttg ttgtaaactt tctttatcat
     2281 ccgcttggta ggcctgacga atgcgtcgtc ccacatctac tttgctactt aaaatagcat
     2341 tcaactgggc ctgagtttca aagagataag catagtttcc agctttttct ttaatgttag
     2401 caagcgtctc agcagcctga gcgaagtgcg gtttgtcctg ttcaggtgtc atgtgttgat
     2461 caagaatcgg acaaagaata tcctgataaa aaacatagcg gttgggattg ataccgctga
     2521 gattgcctgg taggtctggt aagaggttgg caaggtcaat ctgcataaaa tcctcaaccg
     2581 ttagaccagt attggtcttg aagtgcgcag acaaaccatc taggtcattg cgatagctga
     2641 gttctgccca gatttgcaag cttggtagga tagagaactg ggcagtttca ccaccattgt
     2701 ctccccaacc agttacgatg acttctttaa tctgattggc acggcaggct ttattagcct
     2761 cgatagccac tagacggcta aaatgattgt gaggtgtaaa gccaatccac ttccaagctc
     2821 cccctgcaaa tgcaaggtca tggctaatct tgtgatgatt gcggaaatta cggttgtatt
     2881 tttcctcgct atcctgataa taatcccagt aaaccagagt cacacggtct ttgagacggt
     2941 ctaggtagac acgagtttcc tctggaattt ccacatcacg gtcgtactgg ccatccgctg
     3001 acatgagttt gaagaacata tcactccaca tctggcagtg gaaaccatat ttgtcagcaa
     3061 tatccagcac gcgctccaag tgttggcaca tgaggagact acgatccaca acaccgttca
     3121 gaatcaggta gcgtcccaaa ccaaccaagt gggcttcgtc catcccgata ttgaccttgc
     3181 gagtcttcag tttagacaac gtggcaaaca tgccatcaat caggtcataa accttttctt
     3241 cgccaatgag aagaatgtcc tctacatcac ggagctcctg cacttccttg acaccccatt
     3301 tgacaaaggc tgacaggtgg gccaaggtct ggatgcatgg tacaaaggtc atgtcaaact
     3361 gttgagcata ggcttcgatt tcctgcaact cctctgctga ataagctcca cggaaatagc
     3421 caaagtaagg ctgcccttca atctggtaag tgtcttccat gtaaagctca aaggttgagt
     3481 agcccatgag agccaagacc tcaatcatct gcttggcaga agccacattc agcaccgcat
     3541 ttcgagaaca gtcaaccatg taagccaaat cttcgtaagc tgcttgttcc tcaatctcta
     3601 ctttatcagc ttctgctaga actgttacca acaaggacaa ggcacgataa agttggtgag
     3661 gtttgcgata ggtcaattga tagtggccac cctcaccctt gatagagata gaggcttggt
     3721 cagactgagt aacagccact tcaacatctg gcagagaaat gtgatttttt aatacttcaa
     3781 tagcttgcgc ttgtttggaa ctaagtcctg taaatcttac cattggtttt cctcctgtag
     3841 ccagttgaca agggcaccgt agagattagc atctgcatga taggtgcagg cttggataac
     3901 gggtgcgacc gtatattctt cgtaagtatc gacaaagtct tcaacagcct tcttgacacc
     3961 ttgaataaaa tctggatttt gactgataga gcctcccaga ctgatgacat ctggatcaat
     4021 cagatactgg atattgagca agccttgcgc caaattgcgg ttcatgcgct caatggcttc
     4081 ttggcaaatg gcattcccag ctgcagcttc ttggtaaatc ttgcgaccgt cccaatcagt
     4141 ctgaccagat ttttcaatca cgtagcgaac catattccca gttgacgcta gttgcgacca
     4201 gttgttgagc ttttcagcag gggcaagggt tgtcatatag ccaaattctc cacccaagcc
     4261 gtggcgacct cggtgcagtc tgccattgat aatcatagct ccaccaatcc ctgtcccaat
     4321 cacgacacag gctgcatttt caagttctgg atgagccagt aattcactga gtccaacgca
     4381 gttggcatca ttttccagat ggacaggaat ctgataagag ctaagcgcct cataccaaga
     4441 aaagccatgg atgtagggca ccgcactgaa gccatcaatc acacctgtct cttgattgac
     4501 cgcacctgga acactcatag caatcccact gtaatcctgc tctgacaagc gctggtctag
     4561 ccaagctagt aaatcctcca agctttcagg cgttggaatg cttgtcttat tcagtatttt
     4621 tccatcagga gtcagactgg caaacttaat tccagtccct ccgatatcaa tcgttgcaat
     4681 ggtcattgtt tttaccataa aaaggggcga atcagcagtt agttcttact ctttcgcccc
     4741 tcctttctct ttatgtttac tttttgaaat tagatttctt cttttttaat caattctgta
     4801 cgaatttctt gtggagccaa tagtcctgaa tgaactggat atggtcgctc aagtaagtca
     4861 agaatggttt gttgatgttc tgatatgcgc acattttctt gactcatatt atagtaacgg
     4921 aggacatagc cttcttcatt ttccgccact ttaaaggctg ttggacaaac ttgcggtatg
     4981 ctgagaacag aatggctcaa gaggctacca gtcgcaacca cgcttccttc ctgtctagca
     5041 agctgaaggc tggtaaacgg tgtctgcaag gctttggcac gacgataggc tgagaagcgt
     5101 tcttgggctt ggtggcattc aatcgcaaat tcgacttcaa actcccgcaa gcattgtgct
     5161 tctggcgttg ggaagtagcc ccagtcacct agctcacctg atgcacgcaa aatggtcacg
     5221 gcaatggtgt tatccccaag gatttcgtat tcattcaatc ccttattgga tacagtcacc
     5281 cctttttcat cgtcatacag actgacaaaa gcttgttggt gttgaggatt ttcaggattt
     5341 tcccatgaag cagctggttt gtttggtcgt gtcaccacct catagatact ttcagaatca
     5401 ttgcttggac gcgtgttatg agtcttgacc aagagacgga tacggtgatc cttggcagtg
     5461 ttagtaaagc gagtcttgaa gcggatttgt ggattgtcaa cgaagacagt caactcagtt
     5521 tccagaggaa tgtttgtcaa ttcttctgac cgtccagcct cacgcttcat aaactcgatg
     5581 ataccttgtt gctcttcttc tagcttttca tccgcactga caggcacggt caattcatgt
     5641 ttgagcaaga ttttagcata gcgatctgtg ttttccaaga cctcgtggcc cttaagctct
     5701 gcaaagattg gctctgttcc ttttggttgg aaatagatat actcgtttcc gatgtcaccc
     5761 cggtcttcaa agcggataaa gtcttcatag gcttcgtgag ttgtcttgtc atagactgtg
     5821 atgttgtcat ccacactcac cgttacgaat ggtgtatcaa tcactccgtt ttggtaaata
     5881 ccctcacggt gttcttgttt tccttccagc aattggaagg ttgtccaaga aagcggtgct
     5941 aggtgaacta gaatggtcac acgcacttgt cgagcaatac gagcttggcg gaacttatct
     6001 tttggtaaat cgtattcaaa attagctccg aggtcttcga ttgtagcctc tacaggacga
     6061 ccatccaagt cctccacacg gtaacttggc aagataagag cagccatctt tttgtagcct
     6121 tctgttgggt gcaattcctt gaaatcacaa gtcgccacat caatcactgt gctgacagta
     6181 tcgaccttat catgcaagcc tgtgttaatg acagtaaaga gatagtcact ttgagcctta
     6241 tccgtagcaa ttttaccctt ccactcgttg agcaaattac ttttaacaaa gtttcctact
     6301 tggttgacct tggcaaaacg cgtttccatc tcgcggtgaa cttcgtccac gctacagcca
     6361 cagatactat catgtggcgc attctgcaaa agtgttttcc aagcataggt caactggtcc
     6421 ttgtggttgt gtccaccagt gataatagtc aagggttcta ccacttgctc taggaggttg
     6481 ctattttctt ggaaggcttg ttttaggtaa atacgggatg aagaagtgtt ggcaagtgtg
     6541 taccagccat ctgtttcctg actggtcaac tcgcctgtaa cagttgataa gtgttcagga
     6601 agcgcacctt ctacagcttg aacatattca tcaaaagaac tatgaacaaa gattacatcc
     6661 gggaagagtt catttgccac acgaatggct tcgctcagat ttttctgtac aggctggtgg
     6721 tcacagccgt tcatcatcaa ccattggttg gtcgaagcgt aggcacgcac atctgacaat
     6781 ttttgtttcc agaaggtcaa ggcctcatct ttgtcaactg gaatttcatt cccgttactg
     6841 taccagttgg caaagagaat acctaaaaca cgactaccat ccacaccctg ccagtacatt
     6901 tctgaaaact gggatgtaaa ctgctcatct tcaaggactt ggttgtcaaa tccaatcggc
     6961 ttcacaccac gaccaaaggc cgccacgtga atgcctgatt tttgaagaat ttgaggcgct
     7021 tgtcccatat ttccaaaggt atctggaaag tagccaatct gggttgattt accccatttg
     7081 gcagcttctt gttgaccaat caaggtattg cggacattgg cttcactgga gatcaagtag
     7141 tcatcctgca agatgtaaaa gggaccaatt ttaagtttgc cctcgtcaat gtagcgttgg
     7201 accttgtcac gattttcagg gcgaatttgt aagtagtcgt caaggacaat agtttgtcca
     7261 tccaagtgga aactcttgaa ctcagggtca ttttcaaaga gatcaaagag attgtcaaac
     7321 aattccacca actgcatacg atggctttca aaaggcaagt accactcacg gtcccagtga
     7381 ctatgtgaga taatatgtac aacaacattt tccatgaagt aaaacctcat tctaaattta
     7441 aattttaacg ttttaaatgt aaacttgtga ttctgataag aatcggttga gctaaagcga
     7501 gccccttagc gaatatccaa gtaatccaag accaactcac agaacatcat gttagcccag
     7561 gagaaccatt cacgagagta gagggtcgga tcatctacat gaaagctttc gtgcatgaca
     7621 cctgtaccac catcgcaggc aaccagctga tccagcaaga attttttctc tgccttatct
     7681 cttgttgtca atccttggat agaaagggcg attggccaga tatagcgata gaaggtatga
     7741 gaacttccga gaccgctagc gtattctcct tggtagaaat atggattttc agagctcaaa
     7801 atggtacgac gagtagcttg atacacttca tcatcgaccg aacagtagcc cagatagggc
     7861 gcagctagta gacttggtac atttggatca tccatgatgc tggcatttcc taggccatcc
     7921 acttcaaaag cgtaaatctt ttcgcccttg ctgttggtgg tgtaagcgta gtttttgatt
     7981 ccttcttgga tttcatcctg aagacgcttg gcatcagcaa taacactctg gctatcagct
     8041 aggtttaatg ctgcgaagat ttcttgcaca taacccaaga ctactacagc aaacatattt
     8101 gacggaatca agtaactata ctgacagcag tcatcgctcg gacgaaaggc tgaccaagtc
     8161 atacctgtca ctgcaaagtc aggtccaaag ccatcattta ccaaggtgtc ttccttacgg
     8221 tccgtatctc ggacaaaacg ataaggagag ttcttgtggt cttgttccac cgtccacaga
     8281 tggagaattt ccttagtcgc tgcgacaaaa gtctcatcaa actgactagt ctcgccagtc
     8341 tctttccaga ggagataagc caactgcaaa ggatagcaaa gcgaatccac ctcatacttg
     8401 cgctcccaga tccagccgtt aaggtctgtg tggtcagtct cgtggtgccc tttccagttc
     8461 tcctcaatgt tgaaggagtt agcataggga tccttgagta ccaaggtcat ctgacgtttg
     8521 accaaacctg caatggtctg acgcaggagg gcatctcttt tagctacatg aaggtagggt
     8581 ctgagttggg ctgtcgaatc tcgaagccac atggcaggaa tatccccagt caagacaaaa
     8641 gttgaaccat cttctaagat ttcaaccgta ttgtccaagg tatcggtgta gcaacgctcg
     8701 aaaacatcca cccactctgg atggtcctta gcccgctctg ctacttcatc tagccattct
     8761 ctaacaattt ctttcgaata aatcatcgtg caattccctc tttcaaacaa gtttcatgtt
     8821 cagtatatag cttttgagac agaaaataca tacacaaatg atagtcattt ttctacaaaa
     8881 tagttatttt tgaaactcct tttctaaagg ccctcttttt ctgatataat gtagaaaaac
     8941 agctaaagga aagactatga aaccactact tgaaaccatc gatacccgct ttggtactgc
     9001 tagcaagcat gccttctctc ggggaaatac cctgccatac acaggcgtgc cttttgggat
     9061 gaattacttt gtgccccaga ccagtgacca gggtggatct tggttttttg atccgcatct
     9121 gcctatcttt caggggattc gattaactca ccagcccagt ccttggattg gggactactc
     9181 ttggctcctt ctgacacctg tcaccagtca actgggggga gacagcctct ttcaccgcca
     9241 gtcttcttat gatatagata aagcttgctt ccagcctcac tatttgaagc tcttttctct
     9301 gcgttatcag attgaaaccc agctcacacc gacttgctat ggtgcttcta ttcgtttgaa
     9361 gcaaaagcaa ggcaaagccc tctccctcta tcttcacgca gcagatgaac tgacagtaga
     9421 gcaagtagat aagcggactc ttgccctgcg acaagaaggt aaaactgaaa ctaacaaaaa
     9481 ttcgctaacg atgttcactg ccctgcaaat gaacacggat attcttgcta tcagccaaga
     9541 agggggagac tggcgaattg acttagcaag tagtcaaacc gagatgcaac tagccacttc
     9601 tttcatctct ccttctcaag cactgcttaa tctacctcaa gaagattttg atagctgtaa
     9661 atcaagtgcc caagcggatt gggaaaatct cctccatcgt tttgacatta tagagatagg
     9721 agaggctgac cgaaccttct ttgaccactg cctctacaga ctcttcctct tcccacagac
     9781 tttttatgag attaatgaat cagggcaagc catccacatg gatctggcta ctggtactgt
     9841 caagcctggt gtcctcttta gcaacaatgg tttctgggat accttccgca ccaccttccc
     9901 cctctttgcc cttatcatac cggagcacta tcaacgcttt ttagaaggtt tcctcaatag
     9961 ctaccgcgat actggtttcc ttccaaaatg gctggctcca gatgaacggg gtatgatgcc
    10021 aggtacactt ttagacggta ttatcgcaga tagcgcctgc aaggacatgg cccctgacct
    10081 agaaggagaa ctcttccaag ccatgctcga aacagccagc aaagccgacc ctctcggaat
    10141 caatggccgc cacggactag cccaatacca agaactaggt tacctctcta ccgaccacca
    10201 cgaaagtgtt agccatactc tagactatgc ctatagtgac ttttgtatcg ccaactgtgc
    10261 caaaaaacta aaaaacatag aaatcgctga aacctacaag gctgcttcac aaaattaccg
    10321 ccagctattt gacgctgaga caggttacat gcgagcacga gacaatcaag gaaactttca
    10381 ccctgacttc tctccttata gttgggggcg agactacgct gaatgctctg ccattcaagc
    10441 tactttaggc gttctccacg acatccctgg cttaatccaa ctgatgggtg gaaaagaaac
    10501 ctttagcaac tatcttttga aagcctgtca agatgctccc ctctttgaaa caacaggcta
    10561 tggttacgaa attcacgaaa tgagcgagat ggctactgct ccttttggac aaatagccat
    10621 ttccaaccag cctagtttcc acattcctta tctcttccgt tacagcgatt accctgacta
    10681 cactgccctt cttatcaaga cgctccgtca gaaagctttt cacccaagtt gggaagccta
    10741 tcctggagat gaagacaatg gtagtctctc tgcttggtac atctggtcag ctctcggatt
    10801 ttatccaacc tgtccaggaa aaccaagcta tgacctcgga atccctctct ttgaccatct
    10861 acgagtctac ctcgctaaag aagataaatg gctggatatc catactaaac aaaaccacaa
    10921 ccattttaac tttgtcaaag aatgccgact ggacaaaaca ctcgtatcaa ctattcaaca
    10981 ccaagacctc ttaaaagctg aacaactaac tttcaccctc agctggttac caagtcatta
    11041 ataaccaaaa gaacctttgc atcgcgcaaa ggttcttctt ttatgaaact tggacttgaa
    11101 gttgatccac aagctgacca tccaccgtta ggttcagata gaaatccaag gttttatctc
    11161 ctgcgaaata cagggttggt agatgcaaat cttttttgct ttcccctgct tggaattgaa
    11221 ggacttgaat ctcatcctga taggcgacac catggacacc tgtccccggt tgaatcgaaa
    11281 tcttagcctc taaagaggag ctactttcac tacgttcaat cctaaaatga agagtctctc
    11341 cctttgccac agctaggctt ttttctgcaa atgctagttc ctgaacaact tctttttttg
    11401 ataatccagg agttttataa agggaaatct tggtcagtag aggcatagat tgtgcttcgg
    11461 taatcatcac gcgcaccttc tgtgcctcta ctagcggacc tcgcaagagg cgcttatgac
    11521 caactgtgaa gcccctgcca aattcctgcc agacaccatc cacctctact tgcacatgga
    11581 aagcagaaat acgttggcct aacttcaaat cttctctcaa ctcaattaca tcaaaagttt
    11641 taggagaacc taagtcgagt tctaactgga tgggcaagtc tgcatcgttt gcccaagagc
    11701 tggtctcaag gccgtctgtc aaatggcgac aagcaaagtc tgcggaaaga gctggaccag
    11761 atacctcagc tcccagagcc aaatcttctt tatagagctc attgcgatag gtcgcaaatt
    11821 cataaagtcg ttcaatatcc tttgcatcaa agagcccagc ttgattcggc ggaatattaa
    11881 gcaagagtgg agttcctcgc cctactgagt gaaagtagat ttcgaccaac tcctcgagag
    11941 acttaggatc ctgatcctca tggtagaacc agcctggacg gatggaaaca tctgcctctc
    12001 cgattgaaaa aatcgtgccc gagggatccc cgtactgaag atagttcagc tctgcttctg
    12061 ttcctagttt atcaggattc accttttgcc acagtggatc acctgcatac cctcgttcat
    12121 tgccaatcca gcggatactg gtgccttctg ttgaaaaaat caagcaatcg ccctgcaggt
    12181 cacgaatggt ttcaaaccat ttttcaaatt cataattaac cttttgcgcg ccctctcctc
    12241 tggcaccatc catccaaacc tcagcgaact taccagcatt cccatagtta ggatttgata
    12301 agatttcctt caactgagcc agataatagg cattgtagtc cgcttctcgg tccacatgat
    12361 agaggggact atgggcatcc cacggtgaca ggtagacccc catatccata tcaaactctg
    12421 tggcagcttg ggatacttca aggagcaagt cgccctttcc tctcctccaa ggactgacct
    12481 taaccgaata atctgtgtga gctgtcggat aaaggacaaa gccatcgtgg tgcttgacca
    12541 ccaaaatcaa ctttttgaag cccgtttcct tgagcacacg aacccactca cgcgcatcca
    12601 actgactcgg gttaaagcgc tcaggatcct cctgtccagt cccccattct tggtcataaa
    12661 aggtattagg accgaagtgg ataaaagctg ctagttcatc tcccagataa gctagctgag
    12721 tctgacttgg taacggtcca tgcggtttga ttttcttcat cgttatatat ctccttcttt
    12781 agcaacttct cttgttcaca aggttatcca caggttgtgg ataataaaaa atgatctaac
    12841 cgccagctct ttccaactcc aactggtagg ctcaggattt tgctcctcaa tgttccctct
    12901 tcaaacagct ctgtcatatg gttctgctaa cacctctcag aatgctactc tccataatta
    12961 ttctcaggct tctttcaggt cattgtgtga agaccattca ctctccagac ttttccacag
    13021 attgtgaata agtgattatt ctaaactatc atctcgtcga ctataacaaa aaataaaaag
    13081 agggtcaata agataattat ctcttcctaa atactcagaa aagattctat aatttgtaca
    13141 attgagcaag aaatgcttct ccaaaatcta tcttcaaaag aatgttgcat ctttttatcc
    13201 tttcacacaa acatttctct ttatacagat ccaagtgata accagatgat aattggacac
    13261 tttttggtta tttgtgtatt ttttgaaaaa ttttttaaaa aaattttata tcctctttaa
    13321 tagaagttta tgcatttgta tatatgtatt ccatcctatg ttgtgtataa taacataaaa
    13381 ttgtgctaaa tatcataatt tatattattt tccgagaaat tatagccatt gacattacct
    13441 aggaattgcg ttataacata catgtaagcg gtacccaaaa tggtgccaag tcaaaatttt
    13501 taaggaggaa aatacatgtc ttcacatcca attcaggtct tctcagaaat tgggaaactg
    13561 aaaaaagtta tgttgcaccg tccaggcaag gagttagaaa acttgttgcc ggactatctt
    13621 gaaaggcttc tttttgatga tattcctttc ttggaagatg ctcaaaaaga acatgatgca
    13681 tttgcccaag ctcttcgcga tgaaggaatt gaggttctct acctagaaca actcgctgct
    13741 gaatcattga cctctccaga aatccgcgat caatttatcg aggaatactt agacgaagcc
    13801 aacatccgtg atcgtcaaac caaggttgct attcgtgaat tgcttcacgg catcaaggac
    13861 aaccaagaat tggttgaaaa aacaatggct gggattcaaa aagttgaatt gccagaaatt
    13921 cctgacgaag ctaaagatct aactgactta gttgaatcag attatccatt tgcaattgac
    13981 ccgatgccaa acctctattt cactcgcgac ccatttgcaa caattggaaa cgccgtatcg
    14041 cttaaccaca tgtttgcaga cactcgtaac cgtgaaacac tctacggtaa gtatatcttc
    14101 aaataccacc caatctatgg cggaaaagtg gatttggtct acaaccgtga agaagatacg
    14161 cgtatcgaag gtggagacga gctagttctt tctaaagacg tccttgcagt aggtatttct
    14221 caacgtacag acgcagcttc tatcgaaaaa cttttggtca acatcttcaa gaaaaatgtt
    14281 ggcttcaaga aagttttggc ctttgaattt gctaacaacc gtaaattcat gcacttggat
    14341 actgtcttca ctatggtaga ctatgacaag ttcactattc acccagaaat cgaaggcgac
    14401 cttcacgttt actcagttac ttacgaaaac gaaaaactta aaatcgttga agagaaaggt
    14461 gacttagctg aacttcttgc tcaaaacctt ggtgtagaaa aagttcattt gattcgttgc
    14521 ggtggtggca atatcgtagc agctgcgcgt gaacaatgga acgacggttc taacactttg
    14581 accatcgcac ctggtgtggt agttgtttat gaccgcaata ccgtgaccaa taagattttg
    14641 gaagaatacg ggcttcgctt gattaagatt cgcggaagtg aattggttcg gggccgtggt
    14701 ggacctcgtt gtatgtctat gccatttgaa cgtgaagaag tgtaatcgct gttcggcatt
    14761 cgtcaataga aaatgtaaaa aatagaaaga ggaaataata aaatgacaaa ttcagtattc
    14821 caaggacgca gcttcttagc agaaaaagac tttacccgtg cagagttaga ataccttatt
    14881 ggtctttcag ctcacttgaa agatttgaaa aaacgcaata ttcaacacca ctaccttgct
    14941 ggcaagaata tcgctctcct atttgaaaaa acatctactc gtactcgtgc agcctttaca
    15001 actgcggcta tcgaccttgg tgctcaccca gaatacctcg gagcaaatga tattcagttg
    15061 ggtaaaaaag aatctactga agatactgct aaagtattgg gacgtatgtt tgacgggatt
    15121 gaattccgcg gattcagcca acgtatggtt gaagaattgg cagaattctc aggcgttcca
    15181 gtatggaacg gtctaactga cgaatggcac ccaactcaaa tgctcgctga ctacttgact
    15241 gttcaagaaa acttcggtcg cttggaaggc ttgacattgg tatactgtgg tgatggacgt
    15301 aacaacgttg ccaacagctt gctcgtaaca ggtgctatcc ttggtgtcaa tgttcacatc
    15361 ttctcaccaa aagaactctt ccccgaaaaa gaaatcgttg aattggcaga aggatttgct
    15421 aaagaaagtg gcgcacatgt tctcatcact gaagatgctg atgaagcagt taaagatgca
    15481 gacgttcttt acacagacgt ttgggtatca atgggtgaag aagacaaatt cgcagaacgt
    15541 gtagctcttc ttaaacctta ccaagtcaat atggacttag ttaaaaaagc aggcaatgaa
    15601 aacttgatct tcctacactg cttgccagca ttccacgata ctcacactgt ttatggtaaa
    15661 gacgttgctg aaaaatttgg tgtagaagaa atggaagtaa cagacgaagt cttccgcagc
    15721 aagtacgctc gccacttcga tcaagcagaa aaccgtatgc acactatcaa agctgttatg
    15781 gctgcgacac ttggtaacct ttatattcct aaagtataat tttagataat aaaccgtcta
    15841 ccaacagcta tgagggctgc gactaatagc tttagtccgg tcctctttta tgtaatggta
    15901 atctattatt tcttataaaa tatgtgaaaa atcattaaat tgaaatctaa acgcattcta
    15961 ttgagtgtga taaaggagaa tttatggcaa atcgtaaaat tgtagtagct ttgggaggaa
    16021 atgcgattct ttcttctgac ccatcagcaa aggctcaaca agaagcttta gttgaaacag
    16081 ctaagcatct tgtaaaattg attaaaaatg gagatgattt gattatcact cacggtaatg
    16141 gacctcaagt tgggaatctc ttgctccaac atttggcatc agactctgaa aagaaccctg
    16201 ccttcccact cgactcactt gtcgctatga cagaaggtag catcggtttc tggttgaaaa
    16261 atgctttgca aaatgctctc ttggatgaag gcatcgaaaa aaatgttgcc tctgttgtaa
    16321 cgcaagttgt cgtagataaa aatgatccag cttttgttaa cttgagtaaa ccaatcggtc
    16381 ctttctattc agaagaagaa gcaaaagcag aagccgaaaa aagcggagcg actttcaagg
    16441 aagatgctgg ccgtggctgg cgtaaggtcg ttgcctcacc aaaacctgtt gacatcaaag
    16501 aaattgaaac catccgtact cttttaaata atggtcaagt cgtcgtagct gcaggtggtg
    16561 gcggtattcc cgtcgtcaaa gaaaacaatg gacatttgac tggtgtcgaa gcggttattg
    16621 ataaagactt cgcttcccaa cgtttggcag aattggttga tgcagacctc ttcatcattt
    16681 tgacaggtgt agattatgta tttgttaact acaacaagcc aaaccaggaa aaattggaac
    16741 atgtgaatgt tgcccagctg gaagaatata tcaaacaaga tcagtttgca ccaggtagca
    16801 tgcttccaaa agtagaagca gctatcgctt ttgtcaatgg tcgtccagaa ggaaaagcag
    16861 ttattacttc ccttgaaaat ctaggcgcct tgattgaatc tgaaagtgga acaattattg
    16921 aaaaaggata agttgtttta ctaataagat gtattctatt tctagtatct ttatatcaaa
    16981 ttagaaatta ttcttgaaaa catgtacaat atttcaaaag atattagttt tttagacttt
    17041 aatatggtaa aacaaatata aatagaaagc gttttcttaa atgtttattt aagaaagtag
    17101 ttggtttttt acactttgtt agacatcagg aggaaaaaca aatgagtgaa aaagctaaaa
    17161 aagggtttaa gatgccttca tcttacaccg tattattgat aatcattgct attatggcag
    17221 tgctaacttg gtttatccct gcgggggcct ttatagaagg tatttacgag actcagcctc
    17281 aaaatccaca agggatttgg gatgtcctca tggcaccgat tcgggctatg ctaggtactc
    17341 atccagagga aggttcgctc attaaagaaa cgagcgcagc gattgatgta gccttcttca
    17401 tccttatggt tggtggtttc cttggcattg tcaacaaaac tggtgctctt gacgtaggga
    17461 ttgcctctat cgtgaagaag tataagggcc gcgaaaaaat gttaattttg gtactgatgc
    17521 ctttgtttgc cctcggtggt acaacttatg gtatgggtga agaaacaatg gctttctatc
    17581 cactccttgt gccagttatg atggccgttg gttttgatag cctgactggt gttgcaatta
    17641 ttttgctcgg ttctcaaatc ggctgtttgg catctactct gaatccattt gcgacaggta
    17701 ttgcttcagc gactgcggga gttggtacag gggacggtat cgtacttcgt ctgatcttct
    17761 gggttacctt gactgctctt agtacttggt ttgtttaccg ttatgcggat aagattcaaa
    17821 aagatccgac taagtcactg gtttatagta ctcgcaaaga agatttgaaa cactttaacg
    17881 tagaagaatc ttcatctgta gaatctacac ttagcagcaa acaaaaatca gttctcttct
    17941 tatttgtgtt gacattcatc ttgatggtat tgagcttcat tccatggaca gaccttggcg
    18001 ttaccatttt tgatgacttt aatacttggt tgactggtct tccagttatt ggtaatattg
    18061 tcggttcatc tacttctgca ctaggtactt ggtacttccc agaaggcgca atgctctttg
    18121 cctttatggg tatcctgatt ggtgttattt atggtcttaa agaagataag attatctctt
    18181 ccttcatgaa tggtgctgct gacttgctca gtgttgcctt gatcgtagcg attgctcgtg
    18241 gtattcaagt tatcatgaac gacggtatga ttaccgatac aatcctcaac tggggtaaag
    18301 aaggcttgag cggtctatct tcacaagtct ttatcgttgt aacttatatc ttctatctac
    18361 ctatgtcatt cttgatccca tcttcatctg gtcttgccag cgcaactatg ggtatcatgg
    18421 ctccacttgg agaatttgta aatgtccgtc ctagcttgat tatcactgct taccaatctg
    18481 cttcaggtgt cttgaacttg attgcaccaa catctggtat tgtgatggga gctcttgcac
    18541 ttggacgtat caacattggt acttggtgga aattcatggg caaactcgta gtcgctatta
    18601 ttgtagtgac catcgccctt cttctccttg gaaccttcct tccattccta taaaatagtg
    18661 agtgaggtga ttccatgaaa atagatataa caaatcaagt taaagatgaa tttcttatat
    18721 cattaaaaac cttgatttcc tatccttcag tactcaatga aggagaaaat ggaacacctt
    18781 ttggacaagc aatccaagat gtcctagaaa aaactttaga gatttgtcga gacataggtt
    18841 tcactaccta tcttgaccct aaaggttatt acggatatgc agaaatcggt cagggagcag
    18901 agcttctggc cattctctgt catttggatg ttgttccatc aggtgatgaa gcagattggc
    18961 agacaccgcc atttgaagca actatcaaag acggctgggt attcggacgt ggtgtccaag
    19021 atgataaagg cccttcgctc gcagctctct atgcagtaaa aagcttgctg gaccaaggta
    19081 ttcagttcaa aaagcgcgta cgctttatct ttggtaccga tgaggaaacc ctctggcgct
    19141 gcatggcacg ctacaatacc atcgaagaac aggccagtat gggctttgca cctgactcat
    19201 cttttcctct gacctatgct gaaaaagggc ttctacaggt caaacttcat ggccctggat
    19261 cggatcaact agagcttgaa gtaggaggcg cctttaacgt tgtaccagac aaggccaact
    19321 accaaggtcc cctctatgaa caggtttgta acggtctcaa agaagctggt tatgattacc
    19381 aatccactga acaaaccgta acggttctcg gagtgccaaa gcatgctaag gatgctagtc
    19441 aaggtatcaa tgctgtcatc cgactagcta ccattcttgc tcctctccaa gaacaccctg
    19501 ctctcagttt tcttgcaaca caagcaggtc aagacggcac aggaagacaa atctttggtg
    19561 atatagcaga tgaaccttct ggtcacctat cctttaatgt cgcaggtctc atgatcaatc
    19621 atgaacgttc tgaaatccgt attgacattc ggactcctgt cttagctgac aaggaagaac
    19681 tagtagagtt gcttacaaga tgtgcacaaa actaccaact ccgctacgaa gagtttgact
    19741 atctagcgcc tctatacgtc gcaaaagaca gtaaactcgt tagcacactg atgcaaatct
    19801 accaagaaaa gactggcgat aacagtcctg ctatttcatc cggtggtgcc acttttgctc
    19861 gcaccatgcc aaattgtgta gccttcggcg ccttattccc aggagcgaag caaacagaac
    19921 atcaggcaaa tgaatgtgcc gttctagaag atttgtaccg tgctatggat atttatgccg
    19981 aagccgtcta tcgacttgca acttaatcag gcaactgttt ctaccaaaaa aaatcgaccg
    20041 attaatgaac tgcaccccaa aagttagaca gaataaatct aacttttggg gtgttttatt
    20101 atgaaattga gttatgaaga taaagttcag atctatgaac taagaaagca aggacaaagc
    20161 ttcaaacagc tttcaaaaag atttggtgtg gatgtttctg gtctaaagtc atctgaatct
    20221 ttgagatgag ctttataaat cgcttttttc agtttttgca ctggtgtttc gataaactca
    20281 aactttttag ccgtggtatt gcctgatttt atagtatatt gaaactagaa tagtacacct
    20341 ctacttctaa aacattttta gaaatcgatt tgactgtcct gatcgatttg tcctgttctt
    20401 atttcatttt actatatttg agccacttcg tctttaacgg ctttattcat aagctcttgt
    20461 aatttttctt tactatcaat tacttctgat tttccgttgt aatttattgt aataggtttt
    20521 aacttaccta atttctcgac acgctcatta atttgatctt ttttgaaggc tgcatatgtt
    20581 tttcctaaga ttttttcgaa aatatattta tcagatagcg gtttgtattc ttcttcagct
    20641 tggtttttgt attgatttga aacataagga acaaatcctt catagtaacc taatgctccc
    20701 ataagttcaa aagcttgttt tctaattcaa accattgcaa ctcagatttc agcttttcag
    20761 ataaatcctg ctcatccaaa taatgacttg aaattagtgc tgaactcgtt tctgtatcct
    20821 gtacaggctg agcacccata ccagcaaaaa ataaactcgt tcctagcaag accgaacaag
    20881 ctcctattgc atatttcctc aaagaaaaac gctgctttct ctcaaattga aattctttca
    20941 tcccatctcc catcattcat tattactgta tattttgtat atcagaaata gtttgtattc
    21001 acaaatcttt ctagttattc ccttatcatt cctaattaag ggagatgaca tacaataatt
    21061 tttagttaaa tgtatatcga tgttttttgt ttttcttaat aaacgcaata caaaaagagc
    21121 ctgttgccaa gctctttata ctcaatgaaa atcaaagagc aaactaggaa actagccaca
    21181 ggttgctcaa aacaccgttt tgaggtagta gataagactg acgaagtcag ctcaaaacac
    21241 tgttttgagg ttgcagatag aactgacgaa gtcagtaaca tctatacggc aaggcgacgc
    21301 tgacgtggtt tgaagagatt ttcgaagagt attagtctat tatttcttct cagcgcgaag
    21361 ggctgacaag atttgtgttc ggatatcatc cacaccattt ggagtatttg gtaaaaagat
    21421 ggtttgattt cctttagagg caaaggtatt caaggtatcc aaatactggt tggtcaagag
    21481 gatagacatg atttgttctt ctgtcatgcc aacattggct tccttgagtt cggtgataga
    21541 ctctgccaat ccatccacaa tcgccttacg ttgttgggca atccccacac catgaaggcg
    21601 gtctttttct gcttctgctt cagctgcagt gacaatttta atcttgtcag cttccgccaa
    21661 ttcttgtgct gcgacccgct tacgttgcgc cgcattgatt tcattcatgg attgcttaac
    21721 ttctgcatct ggttcgacct tggtaatcaa ggttttcacg ataatgtagc cgtaagtggt
    21781 catttcttct gctacttggt gttgaacctc aagggcaatc tcatcttttt tctcaaacaa
    21841 ttcatccaag gttaattttg gaacagaaga gcgaagagca tcttcgatat aagatttaat
    21901 ctgagattct ggacgtatga gtttatagta agcatctgtc acgctctgct cgttgacacg
    21961 gtactgagtc gctacattca tcataacgaa cacattgtcc ttggtcttag tctcaaccac
    22021 aatatcactt tgcaacaagc gcaactgaat ccgtgctgca atcgagtcaa tcccaaaagg
    22081 caagcgaata tgaataccgc tattagcaac cttttggtat ttcccaaagc gttcaataat
    22141 cgccaccgac tgctgacgaa ccacataaac tgtactcagt gtgactatca ccaataggag
    22201 cacacaaaca atcagaaaaa tcatgaaaaa tattgccata atggaacctc cacaagtatt
    22261 tttctagtat tatagcacat ttaaagaagg ctgtgccgtt tttactgcga tttttcctga
    22321 aatgtcaata attagaggtg aattgtccta ttgtcgtcca atctcttgct aaaataactc
    22381 tttataaaag gcaatcgttt cttctaaggt tggcataaat ggatttcctg gtgcgcaggc
    22441 atcaatcaag gcattcttag aaaggtattc aaagtcgaaa tctttttctt caataccaag
    22501 ttcagtcagt ttcttaggaa tacctactgt ctcagaaagc ttctcaatct cagcaatcgc
    22561 ataatcggca cattcttgat ctgatttacc ttctacatga agtcccaagg ctttggcaac
    22621 attgcggaaa gcttctggta cacgtttagc attttcacgt tctataactg gtagcaacat
    22681 ggcacagcac acgccatgtg gcaaattata tactgcaccg agttggtgag ccattgagtg
    22741 aacataacca agaccagcat tattaaagct cataccacca aggaagatgg cattgaccat
    22801 accttcacgc gcttcaatat catgtccatt ttctacagca cgaggaagat attccttgat
    22861 aagctcaata gcaccaatag acagtttctt ggttacatca taagcaccag gtgttaccaa
    22921 agcttcaaca gcgtgagtca gagcatccat accagtagca gcagtcaggc ctttaggttt
    22981 ggaaagcatg agttccgggt cattaacaga gataagagca aggctattct tgtcaaccat
    23041 taccatctta accttgcgtt cttcatcagt aatcacatag ttaatagtga tttctgcaga
    23101 agttcctgcc gttgtattga tagccacaac tggcaagcct tttttagcag acttatgaag
    23161 cccttcataa tcctgtggtt ttccaccatt tgtagccatg atagaaatac aactagctgc
    23221 atcctgtgga gaacccccgc caagactgat gataaagtca cagccatgtt cttgcaaagc
    23281 agctacccca tctgtgacat ttttacaagt agggtttggc tctacatcgc taaagatgac
    23341 atattcgatt ccttctgtat ccagtggttt taaagtctta ggtaaaatat cactgccttc
    23401 gatgtactta tctgtaacca aaagtgcctt tttatagcca agttccttga tataaggacc
    23461 cacttcattt acaacacctt ttccaataag gttgactgcc ggaacataaa atgtagccat
    23521 ataattttcc tcctgcgcct ccgcgctatc tatttaacat cattatatta ctttttgtat
    23581 tcgtttttaa ttatttgtga attgtttaac aaatcttttt aaaaggatag gagggacgta
    23641 agttcacctc ctatataaac aagttttatt tatgtagtgg tcccaacaac tgacatgcac
    23701 aatagtctgc tgattctaga tcttctgttc caaataagga ccaattttta ggtctaaaga
    23761 tatcttcctc aggtacatta tgcatattga caggaattct caacatagaa gccaaggtaa
    23821 tcaagtctgc tccaatgtgt ccatatgtta tggctccgtg attagctccc caattattca
    23881 tgacgtcata gacagacttg aaagcacctt ttcctgtcaa acgtggagca aaccaagtag
    23941 ttggccatcc tggatctgta cgattatcta aagtatggtg aacatcttca ggaagttcaa
    24001 gtgtataacc ttctgcaatt tgtagcactg gaccaacccc ttttagaaga ttgagacgta
    24061 ccattgttac tggcatatcc cccttcgtca agaaacgagt tgagaatcct cctccacgga
    24121 agtattcgcg gtttgctggt gggaagtctg tattttcaag catagcctgc acttcacttt
    24181 cttccaactc ccagaatggt ttcataacag gtttgccatc tcgagtagct tgacctgtac
    24241 catccaatgt acaagaacca gagttgatta gatgtaagaa gccagctgca gcacgaccct
    24301 ctaaagtatg tcccgttaca cgtttaacag cctctgggct ccaataagta cgcacatcag
    24361 caaagatttg tggagtattt gttaatagat aattaaagag catagacaca ccatttagtg
    24421 aatcattctc tgtcgcaaat acaaatggtt ttcgaatacc attccagtca aactgagtat
    24481 tgaggaaagt ttccataaaa tctccatttg gaaaatggtc tgtccactga cgttgacctt
    24541 ggaaaccagc tactaaagca tggtgaccaa ctgcttcttc ctcaaaacca agttcagcaa
    24601 gtcttgggtt accaaccatt aagtcacgtc caatcatgaa catcttaata acaaattccc
    24661 attgtctatc tttttcttca cggcttaaaa caaggtcttc acggttatgg tcgaatcctt
    24721 cttttacgtt ttctttcacc catttgagcg cacgttcgaa ctcttcaggg tcgtaaatac
    24781 tacggtccat acggcgcgtg aactccgtca tatctaccga ttcatttcgc attcctaggt
    24841 attcttggaa gaaatccgga tttacaatag aaccaccaat ccccatcgaa acactaccca
    24901 ttgataggta agcagtgtct ctcatcaagc cagttgcaag agctgcacgc gcatagcgta
    24961 aaagtttttc tttgacatct tctggaatat ctgtgtcact agcttcctga acatctcttc
    25021 cataaatccc aaaggctgga atcccttttt gagcatgtga agctagtaca gctgcaagat
    25081 agacagctcc tgggcgttct gtcccattaa atccccaaat agcatgagga atatctggag
    25141 acatatccat agtttcacta ccataacacc agcatggtgt aactgtaatt gttgcgcaaa
    25201 catttgattt tttaaacaac tcatgggaag ctgcagcctc tggtacacgg ccaatagtag
    25261 atggagaaat cacgcattcc acaggttccc catctggata tttcaatgtg cttgaaatca
    25321 aatctgccac acttttagcc atgttcattg tttgcacttc aagtgattcg cgtacacctt
    25381 gacgacgacc atcaatagtc ggacgaatcc caatacgtgg atgttgaatc atactatttt
    25441 cctcttattt ttctgtttct ttcatactac catctccatt atatttacgg tagcctgggt
    25501 gataattttt aaaattactt ataagtataa acctctacct ctgctaaact taaaggaatt
    25561 cctgcgcttc tcaaagaaat tctaaccttt tttcccttca gtccttttaa gtctatggat
    25621 agattattgc tttctaaact gtcaatatgt tgtgtaaaaa cttcataatc atttgaatca
    25681 tatataataa tatcaaaatt tgataatctc tgaatttcag catctgttct attataaata
    25741 attaactttt ctaattcttc ggtttgtgct aaatctatct cccaccatga aggagaatct
    25801 tctttggtat gagtcactga atgatgacca taatctccgt ttttatttcc atcaactgct
    25861 agacgagaaa aaccattgta atccgttgaa ctctgtcgag tttcttttgt taatgcaata
    25921 ttagaaagtt tcttcggagt tctctttgaa ccatagactt caacttccgc taaacttaac
    25981 ggaatagctg ctgattttaa ttctattttg actagcttag cccctacaga aggtactttt
    26041 aaagataaca aaccatcaac tactttatca aaatgtcttc taaaaacttc ttctcctgat
    26101 gaagatagaa aaataacatc aaaattagat aaacgctgag gttcggcatc tgttctatta
    26161 taaatatcaa ctttagaaac cgtaaactct tctcccagat cgacctgcca ccaagcgtta
    26221 gaatctgcct tagtatgagt caccgaatga tgtccgtaat ctccatcttt attaccatca
    26281 actgctaaag cagctacacc ctcataattg gttgaacttt gagtagctac cttatttgta
    26341 gagactactt tatcttctgt tattttatct atatcctctt cagattgctt accatctgat
    26401 tctctaaaaa cctctacttc tgctaaactc aaaggcactc cactcgttag taatttaact
    26461 ttaatatacc ttgctccttt ttctttgaaa tctagactaa cagattcacc cgacaaatta
    26521 ttaacatgtt tcttagcaac ttcgtttcta ttattgtcat atagaatcac atcaaaatta
    26581 gatagacgtt gagtctcagc atctgtgcga ttataaattt taacaagccc aactttatcc
    26641 atttttttca aatcgacttc ccaccaagag ggattatctg ccctagtgtg tgttaccgaa
    26701 ccagagttaa aattaccatt tctgttacca tccacagctc tattagggtc tccattgtaa
    26761 tctacagaac tttgtgttgt tggttttgca tatgctatat tcaaattacc atcattaaat
    26821 ttatctggtg ttgggaaaga gactggacct gtcccctcag tatttataga catctctacc
    26881 attccattat gattaaccgt aatggtataa acatgggtat tctcatccca attttctgta
    26941 taagtataat gatttttatc gccacttata tttatctgag gtttatgacc agtatgccct
    27001 tttaatgtaa gtgttgttgt cctaaagtca ttatctacag gattgatgga ataatttttg
    27061 cttatccagt tcgctaactc taattcttct ttcttccaac tttttgatgc atcaaaattt
    27121 cctgataatg cccacatagc tgtcttatct gtcctgtaat tattcaataa aatatgtaaa
    27181 ttatttggat tttctttaac aacagcgtaa gtatgtggcg tcaaatctaa cgataacgac
    27241 tttgtattat tagtatacat aggcaacatt acttgctgat ttttattgat attagcatta
    27301 ctattataaa tataccagga attacctaca cgctgagcat acccatctcc ttcataaagt
    27361 tttggataaa gcgagtttaa atagttgact ttactagaca attcctcact atttttagtc
    27421 aaaatttttg cattagggaa tatagatgaa atcttttcct tatcaatttt ctcatgtatt
    27481 acaggaagaa tatgatatct cccattatta tataaaggca ttgtttcatc attcgaataa
    27541 agtccttgat aaaatccgtt taatgaacta atcctacctt ctccattcca aaatacagct
    27601 tttgttctat ttacaacttc ttccttactt ggagctggat tttgtatagc atgtctaaag
    27661 aaaggaataa tacctttagt aaatgctgga gttggtacat catttgtcat aaatgtatac
    27721 gcggcacatt cgaaattata aactgtgcct cccccagtat atacattcat catttccata
    27781 gcaatcattg attctggttc cgatgcatag gatctcatat ccctagctct tccagtttca
    27841 aatgtgtttg tataatgttt ttcccaccat ttccatgtat ctgttgatga gccccagtta
    27901 tcacataagc cactcaacca aaatccacta acgatagaat ctgtacccgc atcatctctt
    27961 attggcgtat ttttagttgc caacaccaaa tttttatgat atttttgact cgcttcaaag
    28021 aatgtcggat cattcataat agtttcccag aaccattttt catgatcatg ccagataaaa
    28081 tgcgctccat atttggcaca aacttccaaa tatttagcac tatgcggagc taactggtta
    28141 ttgtaaatcc aataattctc aatatttaaa acacctttta acacactata cttttggaat
    28201 tgttcatcta accactctgg aggaactgta ttacgctctc cagccgacat aataaccaag
    28261 aaaactggaa tgtttagact ttgagcttcc tccaacatct tgacatacca ttctctcaat
    28321 tctttcgtat ctcgtggaat acaacttgtt ggtttacaga cttttgcagg atgaagttca
    28381 atcgctgcat atggctttac atcttcagga atagcttccc atgctccctt caatgtgttc
    28441 ccccaccata atccgttacc attatcattg ccatacaacg gagaaagcaa caatggcgat
    28501 tcattattaa tcgtcgttct catttgaaca cgattatcag ccttaactcc tgctaacaat
    28561 ccaaaacata acatcccaat acatacaaac aatattgtga ttaattttct ttttattttt
    28621 tctttattca tgacttatac ctaaaaattt aactcaataa ttttttctga attaaatacc
    28681 aattcaatat tttgtacatc agtatttttg ccatatattc ttactcttac tttttgatct
    28741 ttgtttcctc cttccaattt taggaatgtt atatccccat ttttccaagc aaacgatacc
    28801 ttatatcctc ctcttactct gaaacctttc acttctcctt ctgaccaagc agaaggtaaa
    28861 gctggaatta gtgataacca attatgatgg ctctgtacta ataattcaca aattccactc
    28921 accaaaccta aattaccatc aatttgaaat ggtggatggt caagaaataa attgccaaga
    28981 gtcgcattat ttaacaaacc attaatctgg ttataagcag gttcaccttg atatagtctc
    29041 gcaaaaaaat gaatcagcca tgcagcactc caacctgttt gtgtactagc atgcaaacca
    29101 cttactaacc aattattaat cgcttgctcc ctctcctgtg aagataaaaa attagcgttt
    29161 gataatctcc tattgatagt gattttagct gcttctgcta attccggagt tttatgaata
    29221 tcaatctcat tataaggata aagcccaaat agaggtgaaa tgtgtctatg cccaggctct
    29281 acttcttcat aatcttctaa ccattcttgg atttgcccat tactacctat ttttgtttta
    29341 ggtagtttct tttttaactc cttcacacga ctaataaaat ccgaattgtc tcctaattgt
    29401 tttgcaatgc caatgcatga atcacaaaaa tatcttagaa tttgattatc aattgtagat
    29461 gatagacaag catttccttc aataccattt tttaagcgat atttattttc cggtgagaca
    29521 cttggacctg tcatcaagta gccatccacc tcaaataaat aatcttcaaa gaaaagaaat
    29581 gcttctttta tcatttcaaa atgttccgta agaatacgct catcttggaa atataaatag
    29641 tgttcccaaa tatgagtaca taaccatgga atagttaata cccaaattgc agcccccatg
    29701 gcatgagatt ggggagccgt atcgccaaaa ccatccgtat tatgatgtgc tgtaaaacct
    29761 ctagctccat acattttctt agcggttagt cttcccggtt ctctcattct ttcgagcata
    29821 tcaaataatg gatattctac ttctggtaaa tcacatggac ctaccatcca ataattcatt
    29881 tgagtattaa tattaatcgt atatttagaa ccccaaattg gatttaattc atcacaccat
    29941 attccttgaa gattggcagg taaaccattc ggttgactag acgatattaa cagatatctt
    30001 ccataatgaa ataacaagtt agtcaagtag ttactatact ttttagtgtt ttcaagaagt
    30061 agattcgttg gaatgctaag acaatcttta ctatagtcta gtttaaaatc aactctatta
    30121 aattgctcct gatatttttt tacatgttca tctttttctg taaagtaatc aatactacta
    30181 aattctccct gaagagaaga aatatctata tttccccaat aatccgtcat tgatttgaga
    30241 taaagaaata cctctgtagc attccgaata actattgtct ctcccaatac acttacttca
    30301 ccatccgtaa ccttagaatg acatactact ttaaactgaa caccttttct acctccagca
    30361 gaggccgaca ttaaaattgt acttgaatcc agtttagata cttcatcatt aaaccgttta
    30421 tttctaccca aattaatgtt taaatttaat gtgttttgaa ctgatgacac tatacggcaa
    30481 cataaaatat tcttattaaa actcgtaaaa tattctcttt ttatttgtaa attacaacta
    30541 ttaggctcaa acacaacatt agaaatagct gtatctaaat ctagctctct ttcatacaat
    30601 gaaagagcac aagactgaat atctatatgc tcaatgtaaa gttccccaag taattcatag
    30661 tggctttgat ctcttggggt agcaaacatt gttaacttta tcaattcttc ggctttctga
    30721 atttctccat ctaaaagata ttcccgaatt tttttaagat gcaatagtga gtctggatta
    30781 tttctatctg actttcctct ataccaaata gtctcatcgt ttagttgaat acattctttt
    30841 gtagctgaac cataaatcat accacctaaa tgaccgttcc caatcggcaa ggcttcattc
    30901 caatttgagg cagctttctt ataccataat ttcactcttt acctctcttc caaaatggag
    30961 gtgatatccc aaataccacc tccacacttt ctactacttc aaaattccta aaacaactag
    31021 gataactcca atagccataa cactaaagat tagtacaacc ggattctttc cttttctaat
    31081 taatttacac attaataaag tcaaagccat agggataaat cctggaatta attttgtaat
    31141 catttcttga acagaaataa caagttcccc ctgcttaaat tctaatccaa aattaatacc
    31201 aaccattgat ggaatcaaac cacccactag tactagccct aatgctgtcg ccatactcgt
    31261 aacgcgattc aatgttcctt ttgtattatt ttcttggata agactagaac ccttagtata
    31321 cccatatttc aaaccgaaat atttaacagg aatattaata atattaaaca atattaaggc
    31381 gataaagata cctaaagcac cgccgtcttt agaataagac gcaccaatac taaaacagat
    31441 aggaactagt gtcagccaga acaaactatc tcctagacca gccagtggcc ccatcaagcc
    31501 aactttaata ccagtaattg aagctgcacc ttcatttcct tcttgttctt ccatagcgga
    31561 agtaactcca agaataaatg gtgccgctgt ttgatgagta ttgaaaaact caaggtgacg
    31621 tttcattgca ggagaagctg aatctttgtc tgggtatagt tttttcaata ctggaagaat
    31681 tgtatataga aaacctaggt tctgcattct ctcatagttc catgaagaac catacataaa
    31741 acttcgtttg aaaacttttt taagttcaac tttagttact ttattattca tcatagatca
    31801 agatcctcct caacatcact acttgaagtt gtttcttgtg gtttacttcc aatcatatcg
    31861 tagaagtatg ctaccgcaat accaactagt gagatcccaa tggttgacat tcctccatac
    31921 acagaacaaa tgaatccaat caacaagaat acccacatat ttttcttgag catcatattt
    31981 aaaagcatac caaaaccaat tgaaggtagt aaagcacctg cagagttaag tccctgcata
    32041 acgattggtg gaaccttagc aaacaagtct gccacatatt cccctccaag cataatagct
    32101 aggaaaattg gtacaatttt aaataacgtg attaaaacca aacttgaata atgataaaaa
    32161 ccgatctttg aataatctcc gtttttagca tcattatcag cttttttcac aaaataaaca
    32221 tctaaagttt ttgcaagaac atccaactgt tgggtagcaa ctgcaattgg aactgctata
    32281 gcgataccag cagtttctcc ttgaccagat aaaataccaa atgcagtacc aataatcgca
    32341 cctgaaatag tatctggtgg agtgtaacct ccaatacctg ttacaccgag ccaagttaat
    32401 tcaagagctg aaccaataag aacaccttgg gtgaaatctc ctaagactaa gccaaccatt
    32461 gcacttgtaa ctaacggacg gtgaatcata aataacggtc cattatagtc aatagtggca
    32521 ataatcccaa ctaatgtcac cagtaatgct tgtgtgaata gcatatactg tacctcctta
    32581 aattattttt ataaaaattt ttccaacatt gtaactttat cattaggaac catttgaatt
    32641 tcaactctcg ttcccttatc aactattttc ttaaaataat atttgtcttc ttcacctaga
    32701 gctacacctc ccgttacctt ttcattttcc tccgttttac tcatctgtcc tacattgagg
    32761 tactccaatt ttaaatttcc ttcaatagaa tcatacacat cttttggatt tgtatatatc
    32821 agcattgttc tcttttttat tggcacagag tttaaaactt ccacaaaccg ttttacacta
    32881 aagaaaacaa tttttaaacc taccggtgca gaaatcttta aaatagattg tcgtgtttta
    32941 tcttctgaga tgcgatcatt aacaatgata acttgctcaa tatcatactt ttttagccac
    33001 gtagtgacaa cttgaccatg taccagacgg tcatcaatac gaacaaactc tatagacata
    33061 aatcctcttc ctcctctacg ttaagttgtt gtttaacatt aaacaaacta ttttgagcat
    33121 tttgaacaat ttcttctaaa ttgatttttg aatcataact tgatattaat tccactagga
    33181 gagggatatt aaaccctgtt acaatatcaa ctgaatccaa atttaaaaac cgtgacaaag
    33241 ccacattatt aggacttcct ccaatcaagt cagtcaaaac gataacctct tgatttgagt
    33301 ctaacagttc atttacttga tttttaaaat aatgttcaaa ctctacaata ttctctccag
    33361 gcatcaaacc taatgtccta actctatcat ttgttgtctc accaacaaac atttctgcgg
    33421 tcatgagagc tccgctagca aaattaccat gactggcaac taaaattcct ctattaaaca
    33481 ttttctcttt caacaactac tcctttctta aggataatat tagcataaag tgaagtttct
    33541 cctgtagcaa caatagcata agctttctta ctacgttcat aaaagtcttc tcttctaaga
    33601 taagtaatcg ttttaagatc tgtaccatga ccttcaatca tctgtctata ggtaccccat
    33661 atcttaggaa tatcatcacc cgaaacaacg ttcataaact gaattgaact atcgacgtaa
    33721 ctatctaatg gcattaaata cagaatggaa tctaataatt ctggaatatt tacgccatca
    33781 caacgaatta gcttatttgc acatgaggca gaaggataat tcgcgtcagc taatactatt
    33841 tcatctccat gtcccatttc cattaaagtc ttcaataaat ctggagaaat atttttcggt
    33901 atatgtttta acattaatat cctccctatt ttctctgacc gtatgtttta aatttttctg
    33961 ccattaaatc catctcctca tcaggaagaa ctactggctc tccaaaattc ttagctaaac
    34021 aataaatttt tgcacaatat tcaacttctt caacaatatt aaatgcattc aataaatttt
    34081 gtgcacctgc taaaattcca tgattcgcta gtaaaactgc tctacgacct tccattgctt
    34141 tagctgcatt cacagccaat tctttcgtac catatgttgc atactcagct acccgaacat
    34201 ctttccctgc cactgcaatc atataatgac tagccggaag tggttctctg agacaagcta
    34261 atactgttgc ataagttgta tgagcatgga taattgcatc gatatcatca cgagtttgat
    34321 attgaatcaa atgcatatac cattcgctag atggcaagcg ttctccctct acaacatttc
    34381 cattaatatc catcactaca atatcggatt ctttgatttc aaagaaatca atacccgacg
    34441 gggtaattgc catcaattgt ttttcacgat cgaaaacact gagattccca cctgttcctt
    34501 tcgtcaaatc tgtttctact agcttcttgc catacttaat taattcttgt tttacatctg
    34561 acattcttgt cctcctcata tcaaaacact aaatattttt caattcacca attatccccc
    34621 cttcttgtac aaaacttaag aagggggagg gaggcaaatt tacaaactct caatcttttt
    34681 aattcttttg ggagtaataa ctctctaatt gtagaaactc ctcaataaca tggtgagctt
    34741 cttccatccc ttttaattgt cccatagcta tgagctgcac aacaatattc cccacagctg
    34801 taccctcagt cgaacctgta agaacctctt taccagttct atcagcaatc atttggttaa
    34861 agtaactggc tctagcacct cctccaatca catatatcct cttataaacc ttatgagtta
    34921 gttcttctaa taactctatc gctttcctat acgtttcagc taggctttca taaacaatct
    34981 taaatagttg tccatctgtc cactctctag tttcatgatg ataagctaga tattctgtca
    35041 aagtcttgtg catatcagat tctgttgcaa attctgttga ttcagtatca atcagaggaa
    35101 gattttcttt ttctttctcc accattgtcc taatatcatc aaaagaatag gctttccctc
    35161 ttcgttcaaa tgaacgtctt agttcctcta tgatccacaa ccctgtacaa ttcttcagaa
    35221 atgtaatctc tccatcttta ccgacttcat ttgtaaatcc ataactgaag gattcggtag
    35281 taagaatcgg tgaagtaagt tccactccaa ccaaagacca agtacctgat gaaataaata
    35341 aactaccttc tgtcttaggt actgagacaa tcgcacttgc tgtatcatga ctacaaacat
    35401 tcacaacagg aatatcgcct aaaccatact ccttttttat ccttccaaga acatttccct
    35461 ctgaaacaat ctcaggaagt aaagatgaat ccaattcaaa tagttttaag atattctgat
    35521 tccagttttg actcctagga tcaaataatt gagttgttga agcaatgctt ttttctgtag
    35581 caaacttacc tgtcaagaga taattaaaca aatctggcat taaaagaatc ttattggtct
    35641 tatagaaaga gtcaggagat tcttgacgtg ccttaaagag ttgaaacaag gtatttatct
    35701 ccataatctg atttcctgtc tctgaataca gtttttctaa ttcagtcatt tcagatattt
    35761 cctttaacac tccctttgtt ctttcatcac gataatgaac aggttgtgat aacagcttac
    35821 cttcattatc aatcagtcca aaatcaactc cccatgtgtc aataccgata gataaaatct
    35881 tgtaactagt attagccaag cggatacttt caagaatttt agctagtaga aagtcaacat
    35941 cccaagataa atgccctttt actctaatag gtagattaga aaagcgattt atttcttcca
    36001 ttactagttt attttcagaa aggtaaccaa cgattgctct tccagaagtc gcacctaaat
    36061 ctattgctaa aaccgcttcc atttttcttc tctcttcgtc cttgattaac tttattataa
    36121 tccccacgtt ttattttttc aatatgtgaa cgcttacttg aataatgttc aaatgttaac
    36181 ccaatattca taaaaataat tcacattcct ccaatttgtg ctataatata gttgaaatga
    36241 taaattagga ggagcttatg ataaaagatg aacgtgtact tgaattgatt gaaatcatca
    36301 aaaagaaaaa aagaattgcc gtaaaagagc tggcagaaat cactttctcc agcacaagta
    36361 ccttacgtcg tgatttaatt ttcttagaaa atcaaggtct tatcaaaaga aagcacggat
    36421 acgtgaccct gtcctctatg aacacaattg aactttctca tcaaatacgg gaaggagaaa
    36481 gtactaggca aaaaagacta atcgctagtc tcgctaaaga ctttattcgg tctggtatgt
    36541 gtatctatct agattctagt actactgtct acgaactttg tccctatctt tctgaacttg
    36601 ataatttgat tatttttaca aatggtttac atactgcaca aaccctatct gaaactgtta
    36661 aagatagctc caaaatcttt atcacatctg gcgaggtcaa acatcaatcc tgttccgtgg
    36721 tcaactatga taaggaaaat tctttattag atcattttaa tatcgattta gcattttgtt
    36781 cagcaagagg tattgatgac caatatgttt atgaagcttc tctcagccaa gctatttcaa
    36841 aaaagaatat tattgacaaa gcccatgaaa ccatcttact gattgatagt tctaaatttt
    36901 acaagactgg attttttaaa attaatcccc tctccaaata cacaaccttt atttctgaca
    36961 ccttgccaaa tcaaaaatta ttagacgcag tcgaattatt tgatggagaa tgggtttctg
    37021 atattcaata aaaatcaaat aggaagctag tctcagcttg ctcacagtac agatttgaaa
    37081 actgatgaag tcagctcgaa acactgtttt gaggttgtag atagaactga tgaagtcagt
    37141 cacatatata cggaaagaca aagctgacat gatgtgaaga gattttcgaa gagtatcgtt
    37201 ctgtctgctg actgatttac aaatattcac cacatgacat tattttctta gcaaccctaa
    37261 aaacagaggc tggacaaaaa ctcaatttca ggagaaaatg gagtaaacct tcccacaata
    37321 aaacgcatag tatcaagttt ttgcacacct gatattatgc gtttttctga ttttaaagat
    37381 ttttctctta gatctgatat ttcaagaatt aagtgacctt gatgatctaa atgtattgtg
    37441 gtatactatt tttgtcatcg gttaccgatt acgttcctat gggttcgtga gaggaggtga
    37501 aactaatgag ccttatcata gagctcgctc ttactattat agcggatgtt atagctggaa
    37561 ttatcttgta tttcgtctgc agatggctag atagtaagga gtagaccgac tgactagcct
    37621 ataaacaccc gttaaatcgc taagatacgt caaaaaagcc cttaactacg gccatagtta
    37681 ggggctttgg tgttctaatg aaccttatca attaactaca ttctagcata taatctacaa
    37741 tagttcaagc attaagtaag gttgatgacc tgaatgtagt gtgatatact atttttgtca
    37801 tcggttaccg attacgttcc ttacggttcg tgagaggagg tgaaactaat gagccttata
    37861 ccagagcttg ctcttactat tatagcagat gttatagctg gaattatctt gtatttcgtc
    37921 tgcaaatggc tagatggtaa gaagtagacc gaatgactag cctataaaca cccgttaaat
    37981 cgctaagata cgtcaaaaaa gcccttaact atggcactag ttaggggctt tggtgttcta
    38041 atgaacctta tacactaact acattctagc atataagccc agatatttca agagttttat
    38101 ttattgttta aagttctgaa aggtctataa tgaagttagc catctagtat caaaaaaccg
    38161 actagctctt atgaactagt cgatttctca tcaatgcgcc aacatttctt gggcgatttc
    38221 ttggccagat aggttatctg ggtagtaggt tggccagttg tccatttctt caaagagggc
    38281 ttcttggctt gtgcctccaa agaagatatg gaaatgttct gccttaactg gggcaacatt
    38341 gtggtcacta aactgaacat acttgaattg tccagcgtca gcatctgtgg cttcaaagag
    38401 aaaacgcacg ccacgattgc ctttcttgta agtcaaaatt ttcttaccga catacttgta
    38461 agtgtatttc ttgctttgtc caccttgaac aaattccata gtattatcag taatgttaat
    38521 cttagtcaca tctgtctgat agccttttgt atagtaagcc ttgtactcag cctgggtcat
    38581 cttaccagtc aacttagcct tgtagtcaaa gacttggtca aacgtgccgt cttcaaggaa
    38641 aggataaact gattgccagt tacctgcata gtcactcaag gtgcggtcct tgacagctgc
    38701 atcctcgaag taaccatttt ggactgtctt ggtatcctct gccttttcag gttcgattgc
    38761 tgggccttct tggtctgttg tttgtttcaa agccttgagg tttttctcca tcacggaaat
    38821 gtagttttct ccagccttgg tgtcctcttc tgtcagactt tctaaaggat tgaggacatc
    38881 agttttgaca cctgcttctt ttgaaagtgt gttagcaagg gcttgtgagg cattttcttc
    38941 aaaatagata taggcgattt tatttttctt gacatactct gtcaattctg ccaagcgagc
    39001 agctgatggc tctgcatctg gagaaagtcc tgagattgcg acttgtttga gtccatagtc
    39061 caaggcaaga tagttaaagg ctgcgtgttg agtcacaaag ctcttttgtt ttgcttgaga
    39121 caagccttct gcgtaagcct tatccaaggc ttgcaatttt tcgatatagg cagctgcatt
    39181 cttctcaaag gtctcttttt tatcaggata atctgctgac aagctgtcac gaatgtgctc
    39241 tactagttta atggcacgaa ctggtgataa ccaaacatgg gggtcaaact catggtgatg
    39301 accttcttct ccatggtcat ggtctccctc ttcttcctcg ccacctggca agagcaacat
    39361 atcgcctgtc gccttgatgg ttttcacttt tttcttatcc aaggtatcta gcaatttagg
    39421 tacccatgtt tccatgtttt cattttcata aacgaaggta tctgcatctt ggattttggc
    39481 aactgccttg gcagatggtt cgtattcatg aggttctgtc ccagcaccga ttaggagttc
    39541 tacattagcc gtatctcctg caacttgctt ggtaaattca tagacagggt aaaaggttgt
    39601 cacgatattg agtttaccat ctgcctgttt ttgattggaa caagccacta aaaacaaggc
    39661 acatagactg gctagtaata agctaatttt tttcacgttc gtctcctatt tgataaaacg
    39721 tcttactaaa ctgattagta taaagacagt tacaaaaata atggtaatac ttgcacttgc
    39781 aggtgtttct gcatagtagg aaatgtaaag tcctgctacc attcccaaaa agccaatcgc
    39841 actggcaagc agcataaccg atttaaagtt tttccccaga cgcagggcaa tactagctgg
    39901 caagaccata atggtcgata ccagaagagc tcctgctgca ggaatcataa gggcaatagc
    39961 cacccctgtc accatgttaa aaagaatgga catggtacga actggcaagc catccacaaa
    40021 ggccgtatct tcgtcaaaag ttaagatata cataggacga agaaagagaa aggtcaaaat
    40081 caaaacaacc gccgcaatga caaagaggga aatgacctgt tcttcactga tagtcacgat
    40141 cgaaccaaag agatattggt ccaaactcat tgaactcgag cttttaccct tgctcatgac
    40201 aatcagagaa acagccagac ctgttgacat gaggatagct gtcccgattt ccataaagct
    40261 cttgtaaacc gtacggagat actccagaaa gaccgccgca atcaagacaa tggcaatagt
    40321 agaaacagtt ggagaaatcc ccaaaaccag accaaaggct acacctgaaa gtgagacgtg
    40381 gctaagggta tcactcatca aactctgacg acgcaagatg aggaaggttc ccaataccgg
    40441 tgagaaaaga ctcatagcaa taaccgccaa aaaggcgcgt tgtataaagt cgtaagataa
    40501 taaactaagc atggcccacc tcctggccat tctcatgaac attgaaacaa cgccatggcg
    40561 agtcttggtt acggactaga tgaatattgc gatccgcata atccttaact tcttcagggt
    40621 catgggtaat catcaaaaca gccttgccat gatgatgggc gctgtggtgc atgagttcgt
    40681 aaaattcatt tttacttcct gcatccatcc ccgttgtcgg ctcgtctagg ataaacacat
    40741 cagggtcaga agcaaacata cgcgcaatca ccgctcgctg cttttgtccc ccagatagag
    40801 accccaagcg tttgtctcga tgttcccaca tgccaactga gtccagacta gccttgatat
    40861 gctcctcatc atgagcattc aaacgacgga accagccttt tctcggatag cgacccgact
    40921 tgacaaattc atagaccgta cttggaaaac cagcattaaa actggcaatt tgttgaggaa
    40981 gataggctat tctcaatttc ttaccttgcg tatttgtctt tgaaatagcc acctttccaa
    41041 tgcgtggttg cagaattcca agactagcct tgatgagcgt cgtcttagcc gctccatttt
    41101 ccccagtcaa ggtaacaaat tccccactat caacactata attgatatgt tcaagaacag
    41161 gctccttatc ataatagaag gacaaatcct ctaccgtaat atatctcatt atttgatttc
    41221 tcctactaaa gcagtcaaaa accgctgaat cactttttgt tcatttggag taaactgagt
    41281 cgccacttgt tcataggtta aaagtgtatg ctcatggtga tggtggtgct cctcagcgat
    41341 tggacgagcc aagtcagtca actgataaaa aatcacacgc gcatctttag aatctttaga
    41401 tgtttccaac atcccttcct tgaccaaaga cttaatggcc ttggtaactg ccgcctgact
    41461 gacattgaga cgacgggcca attctgaatt tgttaaagat tcctctgaca agagcataag
    41521 gatatgctcc tgagtattgg tcagggccac ctcgctagtg caatgaccta ttaggatttc
    41581 atgctgattt tccgcctgca aaatcacctc attcaaaaaa gcattgatat cctttgctag
    41641 ctgtctcata tctgactcct ttccttttag acttctcttt tttaagagaa aaatactatt
    41701 ctttgacatt ttgtttacca gttaattata tcacaagcaa aaaaagagtc aagaaaaaac
    41761 gtgaaaacta gtttcattct tgaactcttc tatattatat tatctattga aattctttga
    41821 catctccatc ataagtcgcc caatctttgc tgaaaaagcg ctcattcaga tggtaagtcg
    41881 gagctggtgt gggattggat aggaaaggat caactgcctt gtcaaaagcc aaccaaccca
    41941 accaaccaag gtgaatggtg tccttcataa agaaaggctc cccgccgtcc ttagaaaaat
    42001 ctgctatatt ggtaaaacct tgactttcta actggtagcg aatcttctgc accgtttgtt
    42061 ggtacatatc ctctcgtaga ccagcatagt ccatccattt tttattaaca ggtggaatga
    42121 taaaaatcgg gtttacctta gatttagaaa actgtgttaa aaccaactgc aagtcattat
    42181 actctggcga cttgagatag gtaaagcttt tctgagaatc ctttaatttc ttcaaatcct
    42241 tcttgatctg ctcattatag aaataatttt ccattcccat ctcattattg gaagtatttt
    42301 tttcagcatc tgctttgaca acatcttcta ttgcctgata agaaaactgg tctggcaaga
    42361 tttttaaata cttagctaca tgcttatcgt agttaacata gcctctaacc gaaaactgac
    42421 caaaaaagga agcttggcgt tcattaaaac gagccaataa ttcaatcatt tcattgtctg
    42481 ctgtcgacaa ttcttcttta cttgccaact tctgaaccag gtccttcata gctacgtttg
    42541 ggaactgttg cagtaagcga gtcgctgcat attgactagc ctgatcccca gattgatgtt
    42601 tcagaaaact agtcaactgg tctccattaa aatactgctg gaaggctgct ggatcatagc
    42661 catttttact gaaccactga ggtgagataa catacacaac ttgtttattc tccagctgtg
    42721 gtaacatctg ttgcattcca aaatattggt taagcgatgc agctcccccc tgtcctaaaa
    42781 gataaggacg gtaggaacga ttgtatttct cagctaatac cgcaggatga gcaccgtcaa
    42841 aacgaagcca ttcactagag ccaaagaagg gaacaaaacg cacatttgga tcagatagtg
    42901 ctctgacttt ttgacttcgc tccttaaaac tatcgatagt agtagccact gctgaacgct
    42961 tttcagctcc tagattatga tgcatctcag taggataaaa gaaaatgagc agaaaaacca
    43021 acaaaccagc gatcaagacc ggtccgaaga tcatccataa gcgtttaagc attttgtagc
    43081 tccacaatac cagctatgat tttattagct gtattccagt cgtcacgacc aaactctgtt
    43141 acagggacac gaatgtcaaa acggttctca atctccacaa tcaactcaac cgttcccata
    43201 ctatccaaga cacctgcatc aaaaagatct tcatccatca tgtcagaaac atcttccata
    43261 aacaactcat caataatttc aataacttct gatttgatat ccatatttta tttcctttta
    43321 ttttttaaac catagattat tcaagaatcc agaaaagatt aagaatgaca acatgacaac
    43381 atggaaagtg acaaccatgc caagcaactg aatccagcga ttctcaggta gggcagcctt
    43441 ccctgctttt ttccgttcct tattgagcgt ttttttcttg cgaacccagg catcattgat
    43501 gaccaagcct agtccatgaa agagtccata ggcgatatag taccaggtca caccatgcca
    43561 aaatcccata atcagcatat ttacaatgta ggccatgctt gaggttacat tacgattttt
    43621 aaagactttc tttctggtta acaccatcac cattcgcata aagacaaagt cacggaacca
    43681 gaaggacaga ctcatatgcc agcgattcca aaactccttt aaatcccttg ataaaaaggg
    43741 cttgttaaag ttgatagggc tacggattcc catcaagttt gagatggcca aagcaaacat
    43801 agaataacct gcaaagtcaa agaagagctc cagaccaaaa gtatacataa ctgccaaggc
    43861 atagagatta aagaagccac ctgactgcaa ggctaaattc ttcagaggag gtagtaaggt
    43921 ctctcctaaa acatgagcta ggataaactt atacaaaaag ccccgcatga tatagcggac
    43981 agattcatcc agcatatcca tcaactcatc tcgctcagga atggcctgat aattttcatt
    44041 aaatcgctta aaacgatcga ttggaccact cgagaaagtt ggcatgaaga gaaggaaacg
    44101 gaggaattcc cagagggtaa aatccttaat cactccatct ctcagctcga tgataatccc
    44161 aaccgaacga aaggtcaggt aagaaattcc caagaaccca agcaaagact gcgttccatt
    44221 gatagctggt tgcaccttga caaagataat cggaagtagg gacagaaaac taactaagta
    44281 gaagacccac ttgccatcct tgctttttcg ataatgcttg tagaaaagca ggagcaatat
    44341 ttcccagcaa aggtaaatac ccaaggcagc tagttgattg gtctttccac ccaccaacat
    44401 ggtgacaata aagaagagac ttaccaacac ttcataccag gcaaagcgtt tcttgaaaaa
    44461 gaggcctata aagatgggca aggttgcagc aatcacataa acaaaatact gaggattgcc
    44521 atatggctct aaatgaggaa gctgttgaaa aaactccatc atctcttatt cacctcgtta
    44581 atcaatcctt tgatgtcaat ctttccattt ggagtcagtg gcaaactgtc tcggtaaagg
    44641 aatttagatg gcatcatgta ggacatcatg atgtctgtca ggtcttcctt gatggccttg
    44701 gtaatatcga tatctcgctc aaactgctca cgaacaccgt cttttaagat gacataagcc
    44761 aatagatttt gtaccttgtg gtccttgtta tagcgcggta ctgcgacagc agattcgata
    44821 aagcgagact tgttgaggtt ttgagagaca tcttctaact caatgcggta accgttaaac
    44881 ttaatctgga agtccatgcg tccgccgtag agaagcaagc cctcatctgt catggttccc
    44941 acatcgcctg tgtgataggc tggcagacct tcaaactcaa agaaggcttc tgctgttttt
    45001 tcaggattgt tcatataacc ttttgaaaca gctggcccag aaacaatgat ttctccctgt
    45061 tcgccatttg gcagtttatt tccttcctcg tcaatgataa aggttggaga atcagccttg
    45121 gtatagccga ttggtaggcg tttgagagtc gctaacatct cgtctgtcac ggcaactgct
    45181 gacagagcta ctgtcgcttc tgttgggccg taagcattga tgatacgggc atttgggaaa
    45241 cgctcgcgca gtttttgagc tgttttgacc gtcaattctt caccatcaaa gtagaaatgc
    45301 gtgattccag gcattttctc actgttgaag tattcagaca acatggccat atctgcaaag
    45361 gatggtgttg atgtccagat agcgattggc aatgaaaaga tagccgcaaa gagttgctta
    45421 aagtcctgag tgatgactga aggaagagtg aaaagcgtac caccaagtgc caaggtcggt
    45481 gcccaataca tgacagacaa gtcaaaagaa taaggtggct gtgccagcat ttgcggacga
    45541 ctcggtgtcg caaattcctt atccgtaatc atccagtttg taaagctgag gagattatca
    45601 tgtgaaatct gcactccctt aggcttacca gtcgtaccag aagtaaagat aatgtagtaa
    45661 ttatcatctc ccttgactgg atgcgtgatt tcatagttat tcccttgggc aaaggcttct
    45721 tgaacctgag ctagatttat cattggtgta gaaacctgct ccaagggaaa ggctgaaatg
    45781 gcaataatca agcttggctc tgctacttct aaaatagctg aaactcgctc caaggccgaa
    45841 tggctatcaa ttggaatgta ggcatgacct gacttagtca gcgctacaaa ggttgccaac
    45901 atttcatatt cttggccacc aaaaacaacc acaggagact tctcaggcaa gcctagttgg
    45961 tcaatgactg cagccaaact atccgaatca gcctttaaat cgccataagt gtgttcctgc
    46021 cccaaaacat tatagacagg atagctaggc tgtgtctgag caaaatgctc aatggtttca
    46081 atcatatctg ctattggttt atttgacaca atagggattc tccttcaagt taaaattcat
    46141 tatagataaa gcttccttga ccctgaccaa gatagctaaa gaagtaaagc agccctagaa
    46201 agataagaaa atacaaggct gtccgaccaa gaaagaggta caattctttt ctctgtttca
    46261 tcaagaaaaa ccattcattt ctgtaatttt tcgctaaaat aagagtgatt cttactagct
    46321 tatttttcta ccattgtacc actttatata gtatcttttc aattgtttac cgtatgtttc
    46381 caatagattt cagcttattt taaggattat acagtttttc tatgtatatt ttcaaataga
    46441 gtgatcctgc ttcaaaactc catttcagga gacaatgaag taaatcttcc cataataaaa
    46501 cacacaatat caagtttttt caacacctga tactatgcgc ttttctgatt tttaaagact
    46561 ttttaaccac tctctcattt aaaataatct cgtctgatat aaattaaaat agcttctatc
    46621 atcagacaaa tggctgatag ccaaaaactg atgctaatac caaaactctc agcaatatag
    46681 ctcattagca aaacaaatac tgaaaatgct aatgtagaaa tcacttcaag aacggaatag
    46741 acattaacta aatgattttc ctctactgtt tcctgaagaa atacactttc aggaacttct
    46801 tttagttgcg ataacatacc aactaaagct gaaaataata aaaatatctg tgcgtttgga
    46861 aaatatagaa tagtcagtgt cactattgcc atagctacaa gaggaaaaag aatactttcc
    46921 catttagcag caaggaactt ttcagatagc ctaaaagcaa ttaagccact aattataata
    46981 ccaatagagt atgctgtatt agaatatccc cagtaacttt ccgttttatt taataactcc
    47041 gttacaaaaa caagtataat ggaagaaacc caaatcgtat ttgaaaaaat ttccaataaa
    47101 tttgctgata caaaaagtct taatctagga tttctagcaa ctaacttcca acctttgagc
    47161 aaaatttcaa gattagtttc tgactctaac acctccactt cagcgttagg aagaaataac
    47221 atcagaaagc tagaaatgat atacaagact aaaatgataa acgtggtagg taacagacca
    47281 attgttgcaa acaagagtcc acctaatccc caacctatca attgaacagc ttcaccagtc
    47341 attgataagg ctgaattagc cttacccaaa tcggtcgcat agcgtggcac aatagcatag
    47401 gaaacgggtg ctgcaaaacc atctagtatg gaaattgcaa caacaaatag ataggtcacc
    47461 aaaggcgcta cggattgcat tacggtaaac attcctacca gtatcgccaa taatatagtc
    47521 tttccaaatt gagataaaga taaaacccta tttagcgcta accttttagt aaccaacgga
    47581 actaaaagac tcgcaacaaa agaggatatt cctattaaga taggaactag tgatgtggca
    47641 attactgatt ttgaaataat gtatatgtta gcaatgattg ttactctaaa gaaaatatct
    47701 gctaaatttg caaataattg agagactaac aacttgacaa atgaattaga catgtagcgc
    47761 tcccccttta gttacaaact ttggaattaa atttgatttt taatatttta gcataaaata
    47821 gcagaaaaca aaagtgaggt tttttacatg aaaaaacaaa aactattgcc tttattattc
    47881 ctaattttag gaggaattat gattatcgtt gttggctagt actaagaagt tagttagtta
    47941 gtagaataat tacatcttca atcgaaaaag agactggaaa aagtcttaaa aatcagaaaa
    48001 acgctcaaat cctttggcgc tctaagcttt tctaatgata gatttagata gtcaaaaaaa
    48061 tatctgaaaa gttcccagga tcgtcgtctc agcatcaaaa atacgtgaaa tttgcataat
    48121 taaaataaat ttcaggggag ttaatcgagt aggggataat ctctagcccc tctcacacca
    48181 ccgtacgtgc cgttcggcat acggcggttc aactaacttt taacgcatgt cgttcaaggt
    48241 aataatccaa acacgaaacc agtccacgtt tttccaggac tggttttgat atagcacgtt
    48301 taagtaccga cttctgagct actaattgat aatggtcgcc ccagccagat accttatctg
    48361 ctatccattt aggaactcct aacttaagca atccccataa tcgtctcgat ttcttcttcc
    48421 attgcttcca gataatcact cgtaggcgag tacgcaagcg ctcatctatg ctggcgacta
    48481 tacttttcat atttcccaat gagaaatagt ttatccatcc tcgaatagac aaattcagtt
    48541 gctcaatacg tcttgttagg tctatactcc atttcctctg tgttagtttc ttcaatttaa
    48601 acttaaatct ccgaacacta tcttgatgtg gacggctttt ccaaccatct gatgatttcc
    48661 agaacccaaa acctagatat ttcaactctc ttggtctggt aatcttagtc ttggtcatgt
    48721 ttactttcaa acctagccgt ttctcaataa aacgactgac tgaatacatc acacgcttag
    48781 aggctgcctc cgtctggttt
//
LOCUS       DBERDK010000007        47156 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.007, whole genome shotgun sequence.
ACCESSION   DBERDK010000007 DBERDK010000000
VERSION     DBERDK010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 47156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47156
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.007"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(98..1459)
                     /gene="dnaA"
                     /locus_tag="ACL73L_000360"
     CDS             complement(98..1459)
                     /gene="dnaA"
                     /locus_tag="ACL73L_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HGS0771409.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYNYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVAMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKK
                     KIK"
     gene            complement(1672..2430)
                     /locus_tag="ACL73L_000361"
     CDS             complement(1672..2430)
                     /locus_tag="ACL73L_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HGS0771410.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IEEARAYESLVEKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKEQQDYFFQRIIEEDISVRKLEALLTEKKQKKQQKTNHFIQNEEKQLRKLL
                     GLDVEIKLSKKDSGKIIISFSNQEEYSRIINSLK"
     gene            complement(2488..3669)
                     /locus_tag="ACL73L_000362"
     CDS             complement(2488..3669)
                     /locus_tag="ACL73L_000362"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HGS0771411.1"
                     /translation="MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNS
                     NNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSE
                     GSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISS
                     EKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLKSEDGQAI
                     STKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINI
                     IEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVVVRSVQSNMPANGHL
                     EKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLE
                     S"
     gene            3852..4331
                     /gene="rlmH"
                     /locus_tag="ACL73L_000363"
     CDS             3852..4331
                     /gene="rlmH"
                     /locus_tag="ACL73L_000363"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HGS0771412.1"
                     /translation="MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTP
                     DKASESENQKILEIEGQRILSKIADRDFVIVLAIEGKTFFSEEFSKQLEETSIKGFST
                     LTFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK"
     gene            4375..4448
                     /locus_tag="ACL73L_000364"
     tRNA            4375..4448
                     /locus_tag="ACL73L_000364"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4409..4411,aa:Arg,seq:tct)
     gene            4613..4738
                     /gene="comC"
                     /locus_tag="ACL73L_000365"
     CDS             4613..4738
                     /gene="comC"
                     /locus_tag="ACL73L_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence-stimulating peptide ComC"
                     /protein_id="HGS0771413.1"
                     /translation="MKNTVKLEQFVALKEKDLQKIKGGEMRLSKFFRDFILQRKK"
     gene            4759..6084
                     /gene="comD"
                     /locus_tag="ACL73L_000366"
     CDS             4759..6084
                     /gene="comD"
                     /locus_tag="ACL73L_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence system sensor histidine kinase ComD"
                     /protein_id="HGS0771414.1"
                     /translation="MDLFGFGTVIVHFLIISHSYHFICKGQINRKELFVFGAYTLLTE
                     IVFDFPLYILYLDGLGIERFLFPLGLYSYFRWMKQYERDRGLFLSLLLSLLYESTHNF
                     LSVTFSSITGDNFVLQYHFPFFFVVTVLTYFVTLKIIYYFHLELAYFDEDYLYPFLKK
                     VFFALLLLHIVSFVSDMVSTIKHLNSFGSILSSIVFISLLLTFFAMNSHKVQMEKEIA
                     LKQKKFEQKHLQNYTDEIVGLYNEIRGFRHDYAGMLVSMQMAIDSGNLQEIDRIYNEV
                     LVKANHKLRSDKYTYFDLNNIEDSALRSLVAQSIVYARNNGVEFTLEVKDTITKLPIE
                     LLDLVRIMSVLLNNAVEGSADSYKKQMEVAVIKMETETVIVIQNSCKMTMTPSGDLFA
                     LGFSTKGRNRGVGLNNVKELLDKYNNIILETEMEGSTFRQIIRFKREFE"
     gene            6081..6833
                     /gene="comE"
                     /locus_tag="ACL73L_000367"
     CDS             6081..6833
                     /gene="comE"
                     /locus_tag="ACL73L_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence system response regulator
                     transcription factor ComE"
                     /protein_id="HGS0771415.1"
                     /translation="MKVLILEDVIEHQVRLERILDEISKESNIPISYKTTGKVREFEE
                     YIENDEVNQLYFLDIDIHGIEKKGFEVAQLIRHYNPYAIIVFITSRSEFATLTYKYQV
                     SALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
                     IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHTQRFYSPHKSFLVNIGNIREIDRK
                     NLEIVFYEDHRCPISRLKIRKLKDILEKKSQK"
     gene            6885..6956
                     /locus_tag="ACL73L_000368"
     tRNA            6885..6956
                     /locus_tag="ACL73L_000368"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6918..6920,aa:Glu,seq:ttc)
     gene            6962..7035
                     /locus_tag="ACL73L_000369"
     tRNA            6962..7035
                     /locus_tag="ACL73L_000369"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6996..6998,aa:Asn,seq:gtt)
     gene            complement(7076..7618)
                     /locus_tag="ACL73L_000370"
     CDS             complement(7076..7618)
                     /locus_tag="ACL73L_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGS0771416.1"
                     /translation="MQESNKRLKTKRTIENAMVQLLMEQPFDKISTVKLVEKAGISRS
                     SFYTHYKDKYDMIEHYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLAALLSE
                     NGTKEIQNFLRNKLHIMLSTDLQKRFMQLNLNTTELEYSSIYLTHALFGVCQTWIAHG
                     KKESSQEITDFLMKMLGDTN"
     gene            7797..9400
                     /locus_tag="ACL73L_000371"
                     /pseudo
     CDS             7797..9400
                     /locus_tag="ACL73L_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518194.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
     gene            complement(9430..11982)
                     /locus_tag="ACL73L_000372"
     CDS             complement(9430..11982)
                     /locus_tag="ACL73L_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HGS0771417.1"
                     /translation="MKSFLKTYRTYFISFIIPVVIMSGVYLSQGIYWNSDNSPLLGDG
                     FHQYVIFDVALRNILHGNSSLFYTFTSGLGLNFYALSSYYLGSFLAPLVYFFDLTNMP
                     DAVYLTTLLKFGLIGLSTFFSLNKLFQSIPQTLKLALSTSYALMSFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTSLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTRKSSVLDFIVISFLAGMASLIMTLPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLLPFILTILFFTLKSIKFHVKLIYIIFFAFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLKRLKELKVWNFLVSLFLVVTGFL
                     ATIYLKSHYSFLTDLNILLTLEFLVVYSLLLLAVIKKFISVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSAYSRDIPAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVNFDY
                     FSPIPYEKTEKIENTNDLISVTSSSNEDAAIQYQIEVPENSQVYLSFINLHFSNDKQK
                     KVDILVNGEKKTFTTDNVFSFFNLGYTKEKKTFNINVSFPGNSQVSFESPTFYRLDTK
                     TFTEAIQKIKEQPVTVSTSKNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQ
                     AQTGFMKVDIPKGKGTITLSFIPNGFITGAICSFTSLLLFGIYNHRRKSSKA"
     gene            complement(12044..13666)
                     /locus_tag="ACL73L_000373"
     CDS             complement(12044..13666)
                     /locus_tag="ACL73L_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGS0771418.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNMSELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREIGNDLLTVENLTVKIDGETI
                     LDNISFILRPDDKTALIGQNDIQTTALIRAIMGDIDYEGTVKWGVTTSQSYLPKDNSA
                     DFAGGESILDWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEVQAKVKELWKD"
     gene            13872..14897
                     /gene="trpS"
                     /locus_tag="ACL73L_000374"
     CDS             13872..14897
                     /gene="trpS"
                     /locus_tag="ACL73L_000374"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HGS0771419.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERAREVAGQTLSEVKGA
                     MGLHYFN"
     gene            15049..16527
                     /gene="guaB"
                     /locus_tag="ACL73L_000375"
     CDS             15049..16527
                     /gene="guaB"
                     /locus_tag="ACL73L_000375"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HGS0771420.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SENLVTAPVGTDLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(16578..17675)
                     /gene="recF"
                     /locus_tag="ACL73L_000376"
     CDS             complement(16578..17675)
                     /gene="recF"
                     /locus_tag="ACL73L_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HGS0771421.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSVQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEEL
                     SISYQSSVNTTDKQNLSESFKIALEKSRSRDLFKKNTGVGPHRDDISFYINGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQDGKAAVNGN"
     gene            complement(17678..18046)
                     /gene="yaaA"
                     /locus_tag="ACL73L_000377"
     CDS             complement(17678..18046)
                     /gene="yaaA"
                     /locus_tag="ACL73L_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HGS0771422.1"
                     /translation="MEYKLFEEFITLQALLKELGITHSGGAIKSFLSEHSVYFNRELE
                     SRRGKKLRIGDKVDIPDMNIDILLTQPTSEEQDEYQADKVEKERIAKLVKKMNKGVKK
                     DKSKPASSPKSQQAPRFPGR"
     gene            18205..19455
                     /locus_tag="ACL73L_000378"
     CDS             18205..19455
                     /locus_tag="ACL73L_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HGS0771423.1"
                     /translation="MELVHGISTHFIQSKKFKTNKITVRFTAPLSLDTIAGHMLSASM
                     LETANQMYPTSQDLRRHLASLYGTDMSTNCFRRGQSHIIELTFTYVRDEFLSRKNVLT
                     SQILELVKETLFSPAVVDNGFDPALFEIEKKQLLASLAADMDDSFYFAHKELDKLFFH
                     DERLQLEYSDLRNRILAETPQSSYSCFQEFLANDRIDFFFLGDFNEVEIQNVLESFGF
                     KGRKGDVKVQYCQPYSNILQEGMVRKNVGQSILELGYHYRSKYGDEQHLPMIVMNGLL
                     GGFAHSKLFTNVRENAGLAYTISSELDLFSGFLRMYAGINRENRNQARKMMNNQLLDL
                     KKGYFTEFELNQTKEMIRWSLLLSQDNQSSLIERAYQNALFGKSSADFKGWIAKLEQV
                     DKDAICRAANNVKLQAIYFMEGIE"
     gene            19452..20735
                     /locus_tag="ACL73L_000379"
     CDS             19452..20735
                     /locus_tag="ACL73L_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HGS0771424.1"
                     /translation="MTKVVFEKKYYPAVKEMIYRTRLANGLTVALLPKKEFKEVYGSV
                     TVQFGSVDTFVTEVDGDVKQYPAGIAHFLEHKLFEREDSSDLMSAFTSLGADSNAFTS
                     FTKTNYLFSATDYFLENLYLLDELVTSAHFTEASILTEQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESISQINLTNLQENFTKFYKPVNMSLFLVGNFDVERV
                     QDYFESKELKDSDFQEVAREKLFLQPVKPTDSMRMEVSSPKLAIGVRGKREVSEADCY
                     RHHILLKLLFAMMFSWTSDRFQKCYESGKIDASLSLEVEVTSRFHFVMLTMDTKEPVA
                     LSHQFRKAIRNFTKDLDITEEHLDIIKREMFGEFFSSMNSLEFIATQYDAFENGETIF
                     DLPKILQEITLEDVLDAGHHLIDDGDIVDFTIFPS"
     gene            20777..21598
                     /gene="rodZ"
                     /locus_tag="ACL73L_000380"
     CDS             20777..21598
                     /gene="rodZ"
                     /locus_tag="ACL73L_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HGS0771425.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKK
                     KKKTSFLPLFYFILFALSILIFVTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHS
                     SSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTL
                     SPKKKSAEATVATKSPVTITLGVVKGVDLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            21609..22154
                     /gene="pgsA"
                     /locus_tag="ACL73L_000381"
     CDS             21609..22154
                     /gene="pgsA"
                     /locus_tag="ACL73L_000381"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HGS0771426.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFIFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIMLIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILAAAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            22151..22978
                     /locus_tag="ACL73L_000382"
     CDS             22151..22978
                     /locus_tag="ACL73L_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HGS0771427.1"
                     /translation="MKSIIDVKNLSFRYKENQNYYDVKDITFHVKRGEWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRQIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALALVGMLDFKKREPARLSGGQKQRVAIAGVVALRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPRELFSRNDLDQIGLDDPFANQLKKSLSQNGYDLPENYLTESELEDKLWELL"
     gene            22963..23802
                     /locus_tag="ACL73L_000383"
     CDS             22963..23802
                     /locus_tag="ACL73L_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HGS0771428.1"
                     /translation="MGIALENVNFTYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQLLNGLLVPSQGSVRVFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPAILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPIKIEEFKES
                     LNG"
     gene            23795..24589
                     /locus_tag="ACL73L_000384"
     CDS             23795..24589
                     /locus_tag="ACL73L_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HGS0771429.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRVKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKAMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            24648..25466
                     /gene="mreC"
                     /locus_tag="ACL73L_000385"
     CDS             24648..25466
                     /gene="mreC"
                     /locus_tag="ACL73L_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HGS0771430.1"
                     /translation="MNRFKKSKYVIIVFVTVLLVSALLATTYSSTIVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLL
                     DMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVE
                     ENSTIVNLLTNTENADKISVKIQHGSTTIYGIIIGYDKENDVLKISQLNSNSDISAGD
                     KVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            25466..25960
                     /gene="mreD"
                     /locus_tag="ACL73L_000386"
     CDS             25466..25960
                     /gene="mreD"
                     /locus_tag="ACL73L_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HGS0771431.1"
                     /translation="MRQLKRVGVFLLLPFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLVYDVYFFHLIGITTLLFILLGAFLHKLNSVILLNRW
                     TRMLAMIVLTFLFEMGSYLLAFMVGLTVDSMSIFIVYSLVPTMILNFLWITVFQFIFE
                     KYYL"
     gene            26054..27232
                     /gene="pcsB"
                     /locus_tag="ACL73L_000387"
     CDS             26054..27232
                     /gene="pcsB"
                     /locus_tag="ACL73L_000387"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan hydrolase PcsB"
                     /protein_id="HGS0771432.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQ
                     SLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLL
                     EQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKV
                     RPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIA
                     CWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGFVTYIYAD
                     "
     gene            27456..28235
                     /gene="rpsB"
                     /locus_tag="ACL73L_000388"
     CDS             27456..28235
                     /gene="rpsB"
                     /locus_tag="ACL73L_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HGS0771433.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAADAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDAVAVEAEFAASETQADSIEEIVEVVEGDNA"
     gene            28314..29354
                     /gene="tsf"
                     /locus_tag="ACL73L_000389"
     CDS             28314..29354
                     /gene="tsf"
                     /locus_tag="ACL73L_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HGS0771434.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVVEGGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKAQLTDDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKAANDFEA
                     EVAATMAAALNN"
     gene            29600..29923
                     /gene="tnpB"
                     /locus_tag="ACL73L_000390"
     CDS             29600..29923
                     /gene="tnpB"
                     /locus_tag="ACL73L_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGS0771435.1"
                     /translation="MMIQLSDLGQVHIVCGKTDMRQGIDSLAYVVKTHFELDSFSGQV
                     FLFCGGRKDRFKVLYWDGQGFWLLYKRFENGKLTWLSTEKDVKALTSEQVDWLMKGFS
                     ITPQI"
     gene            complement(30064..30351)
                     /locus_tag="ACL73L_000391"
                     /pseudo
     CDS             complement(30064..30351)
                     /locus_tag="ACL73L_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            30333..30862
                     /locus_tag="ACL73L_000392"
                     /pseudo
     CDS             30333..30862
                     /locus_tag="ACL73L_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_050084071.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(30937..31857)
                     /gene="cysK"
                     /locus_tag="ACL73L_000393"
     CDS             complement(30937..31857)
                     /gene="cysK"
                     /locus_tag="ACL73L_000393"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HGS0771436.1"
                     /translation="MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKPGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVFAVEADESAILSGEKPGPH
                     KIQGISAGFIPDTLDTKAYDGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIE
                     VAKKLGTGKKVLALAPDNGERYLSTALYEL"
     gene            complement(32106..32741)
                     /locus_tag="ACL73L_000394"
     CDS             complement(32106..32741)
                     /locus_tag="ACL73L_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HGS0771437.1"
                     /translation="MEFRTIKEDGQVQEEIKKSRFICHAKRVYSEEEARDFITAIKKE
                     HYKATHNCSAFIIGERSEIKRTSDDGEPSGTAGVPMLGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYAGSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHGLMEL
                     DTNFTDQVDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLREVEVPVNLV"
     gene            32797..34095
                     /locus_tag="ACL73L_000395"
     CDS             32797..34095
                     /locus_tag="ACL73L_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGS0771438.1"
                     /translation="MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     STILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQVVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
                     LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPML
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLAEILQEQFPNEKIGFVS
                     SVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGG
                     RVGRSMDRPTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL"
     gene            34092..34754
                     /locus_tag="ACL73L_000396"
     CDS             34092..34754
                     /locus_tag="ACL73L_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HGS0771439.1"
                     /translation="MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEEN
                     CPNCMKTELSTKCQDCQFWCKEGVEVSHRAIFTYNQAMKDFFSRYKFDGDFLLRKVFA
                     SFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREERASSS
                     KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSL
                     VR"
     gene            34834..35382
                     /gene="raiA"
                     /locus_tag="ACL73L_000397"
     CDS             34834..35382
                     /gene="raiA"
                     /locus_tag="ACL73L_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HGS0771440.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKN
                     KVATGQLFTDALVEDSNIVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVED
                     QTTNVIYRREDGEIGLLEVKES"
     gene            35518..37491
                     /locus_tag="ACL73L_000398"
     CDS             35518..37491
                     /locus_tag="ACL73L_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HGS0771441.1"
                     /translation="MKKFYVSPIFPILVGLIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTALLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDVSR
                     EQAITDELVTSRPVIGIVSVDNYDDLEDETSESDISQINSFVANFISEFSEKHMMFSR
                     RVSMDRFYLFTDYTVLEGLMNDKFSVIDAFREESKQRQLPLTLSMGFSYGDGNHDEIG
                     KVALLNLNLAEVRGGDQVVVKENDETKNPVYFGGGSAASIKRTRTRTRAMMTAISDKI
                     RSVDQVFVVGHKNLDMDALGSAVGMQLFASNVIENSYALYDEEQMSPDIERAVSFIEK
                     EGVTKLLSVKDAMGMVTNRSLLILVDHSKTALTLSKEFYDLFTQTIVIDHHRRDQDFP
                     DNAVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGRKLGSDVLIAEAKDTKCY
                     DTVVISKAADAMLAMSGIEASFVLAKNTQGFISISARSRSKLNVQRIMEELGGGGHFN
                     LAAAQIKDVTLSEAGEKLTEIVLNEIKEKEKEE"
     gene            37488..37940
                     /gene="rplI"
                     /locus_tag="ACL73L_000399"
     CDS             37488..37940
                     /gene="rplI"
                     /locus_tag="ACL73L_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HGS0771442.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQAPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            37984..39336
                     /gene="dnaB"
                     /locus_tag="ACL73L_000400"
     CDS             37984..39336
                     /gene="dnaB"
                     /locus_tag="ACL73L_000400"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HGS0771443.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDRGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEIIAQAEKGLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            39338..39604
                     /locus_tag="ACL73L_000401"
     CDS             39338..39604
                     /locus_tag="ACL73L_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HGS0771444.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            39693..41039
                     /gene="cbpD"
                     /locus_tag="ACL73L_000402"
     CDS             39693..41039
                     /gene="cbpD"
                     /locus_tag="ACL73L_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HGS0771445.1"
                     /translation="MKILPFIARGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSGG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEIPAAYGNANEWG
                     HRARREGYRVDNTPTIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRI
                     IKANTMTGFIHFKDLDGGSVGNSQSSTSTGETHYFKTKSAIKTEPLVSATVIDYYYPG
                     EKVHYDQILEKDGYKWLSYTAYNGSYRYVQLEAVNKNPLGNSVLSSTGGTHYFKTKSA
                     IKTEPLVSATVIDYYYPGEKVHYDQILEKDGYKWLSYTAYNGSRRYIQLEGVTSSQNY
                     QNQSGNISSYGSNNSSTVGWKKINGSWYHFKSNGSKSTGWLKDGSSWYYLKLSGEMQT
                     GWLKENGSWYYLGSSGAMKTGWYQVSGKWYYSYSSGALAVNTTVDGYRVNSDGERV"
     regulatory      41122..41224
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            41295..41585
                     /locus_tag="ACL73L_000403"
     CDS             41295..41585
                     /locus_tag="ACL73L_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HGS0771446.1"
                     /translation="MKASIALQVLPLVQGIDRIAVIDQVIAYLQTQEVPMVVTPFETV
                     LEGEFDELMRILKEALEVAGQEADNVFANVKINVGEILSIDEKLEKYTETTH"
     gene            41548..42306
                     /locus_tag="ACL73L_000404"
     CDS             41548..42306
                     /locus_tag="ACL73L_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS0771447.1"
                     /translation="MRNLRSILRRHISLLGFLGVLSIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLIACLMAVLMDSLTWLNDLIYPMMVVIQ
                     TIPTIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRA
                     KPWQILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQY
                     DTMFAIIILVSIISLLGMKLVDISEKYVIKWKRS"
     gene            42345..43352
                     /locus_tag="ACL73L_000405"
     CDS             42345..43352
                     /locus_tag="ACL73L_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGS0771448.1"
                     /translation="MKKTWKVFLTLVTALVAVVLVACGQGTASKDNKEAELKKVDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            43352..44080
                     /locus_tag="ACL73L_000406"
     CDS             43352..44080
                     /locus_tag="ACL73L_000406"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0771449.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENPKGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AEAISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLQLTTLIITHSIEEALNLSDRIYILKNRPGQIVSEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLDK"
     gene            44199..44657
                     /locus_tag="ACL73L_000407"
     CDS             44199..44657
                     /locus_tag="ACL73L_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HGS0771450.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRELLYSIGERVSQEIYEDIL
                     QLLVEQELMTKQEMNLLESVALDRVLGEEAPVVRANMLRQIIQEVDRKGK"
     gene            44659..47091
                     /locus_tag="ACL73L_000408"
     CDS             44659..47091
                     /locus_tag="ACL73L_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HGS0771451.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYEMDRLEEVALELTETDYSQDETFTELPFSRRLQVLFDEAEYVASVVYAKV
                     LGTEHVLYAILHDGNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQARSGKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALALGLAQRIASGDVPAEMAKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     TLRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQ
                     ITDEAVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKAKHVKADDSDLSPADKAL
                     MDGKWKQAAQLIAKEEEVPVYKDLVTESDILTTLSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSSDHMQEVVKIMVKPLVASLAE
                     KGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGSTLKI
                     GVKAGQLKFDIA"
BASE COUNT        14748 a         7724 c         9897 g        14787 t
ORIGIN      
        1 tctgtggaaa acagccttta aacaagtcat atcaagtttt ttagcttgtt taaaaaatgt
       61 ggataaaaaa gataaaaaac taaaagttat ccacaaatta tttgattttc tttttgattg
      121 attcaatttc taaacgtaaa ttatcgtctt gatcaatcaa agattttatt ttggcatggg
      181 catgaatgac tgtggtatga tcttttcccc caaattcctt cccaattttt ggaagactat
      241 tatctgttag ttctctagat aaatacatgg ctacttgacg ggccaaaaca atattttgaa
      301 ggcgtctact tcccttcatt tctttgatac taacaccata aaagttacca acttcagttt
      361 ggattttatc aattgggatg acgagcattt ggctaacatc ttgtttgcgg gctctaatgg
      421 cttctgcagc aatatcaata gtgatatcct tgattttttt tactctggca attaaagtga
      481 tgtcgttgat ggctccctca agatctcgaa catttgaatc aaattgccca gctaggtatt
      541 ctagagtatc actttggaaa ttgtagccta aatgttccgt tttactttgt aaaatggcaa
      601 tacgtgtttc aaagtcaggg ggtgtgatag tttgtgtcaa tccccaacta aaacgcgtga
      661 caagcctctc ctcgagccct tctagatgtt ttggactacg atcactcgtt aggacaatct
      721 gtttttgctt gtcatgaagg gcgttaaagg tattgaaaaa ttcttcctga gttgcgactt
      781 tttttccgct gagtgactgg atatcatcga ttaacaaaag atcaagacta cgataggtct
      841 ttttaaactt ttccatttcc ccaagtctta ggtgatcaag aaagtcatta ataaagcttt
      901 cggcagggat atatttaaca cgcgcattag gaatattttt tagaatttca tttccaatag
      961 cgtttaataa gtgagtctta ccaaggcctg gtcctccata gataaaaaga gggttatagg
     1021 tcagagccaa atcttcagag acagctaaag cggctgacac agcccaaaca tttccatccc
     1081 cttgaataaa gttatcaaag gtatactttt cttttaatcc cgtatctgaa taaggaatag
     1141 atactaactt tggactatag ttataaagag ttaaatttgt agcttcttca acttgtgagc
     1201 tagtcgtatc ttgaggtttg gtgaaaatat agtggggagt tatttcagcg tcataaattt
     1261 caaaaccagc tactacaata atatctttta gttgtttttc ccagaccatt tccatttcag
     1321 agcgaggtag aaatatagtg gcaacatttt cctctacctt gatgagttca gcttgaatag
     1381 catagaaatc atacatggat cgagtcagtc tttcttgtgc aaattctaat atacgattcc
     1441 aaaattgttt ttctttcaat cctttctcct cctttactat ttaaaagttt aatactagag
     1501 atattttacc atagatagca agaattttcc acaagctgtg aaaaataatc cacaatgtta
     1561 ccaaacttta tccacaggtt ggggataaaa gaagaaatta ttgatttatt aagcttttta
     1621 ttgaaaaaca tagtggataa ccttgtgaga taaaaaaata aaagaacagc cttatttcag
     1681 gctgttgata attctactat attcttcttg atttgaaaaa gaaataatga tttttccact
     1741 gtctttttta gatagtttaa tttctacatc taatccgagt agttttctta actgtttttc
     1801 ttcattttgt atgaaatgat tagttttttg ctgtttcttt tgttttttct ctgtcagaag
     1861 agcttctaat ttccttacag aaatatcttc ttctataatc cgttgaaaga aatagtcttg
     1921 ttgttcctta tttaacccaa ctagggaacg cgcatgggct tgtgatagtt tgccattttc
     1981 tacttctgaa agaatctgtt ctggcaagga aagtaaacga atggagttgc tgatatatgg
     2041 acgagacttg cccatcttat ctgcaatttc agcatgggtg aatcctttct ctacgagaga
     2101 ttcataggcg cgtgcttctt ctattgggtt taaattttct ctctgtaaat tttcaataat
     2161 ggactggacc atcatctctt ggtctgaaat ctgtttaaca acagctggga tagaccgtag
     2221 accagctaaa agtgaagccc gatagcgtct ctctcctgca aggatttcat aaccaataac
     2281 aggagattga cgaacaataa tcggttgaat gaccccattt tctttgatag actgtgctag
     2341 ttcatctagt ttttctctat caaattcttt tcggggttga tagggatttt tttgtatatc
     2401 tgtgatagaa atcatttcaa atttttccat gattctacac taacacatct tttctcttat
     2461 gtaaagcttt ctttacatag atgtcaatta agattctaaa tcaccagaac tcttgttaag
     2521 tttgatagag gtagtttctt ctttcccgtt acgatagtag gttatcttaa tggtgtctcc
     2581 gatagaatgg ttgtaaagag cactttgtaa gtctgttgat gaagcaatct ctttgtcatc
     2641 tacttttgta attacatcgt atttttcaag gtgaccattg gcaggcatat tactttgtac
     2701 cgaacgaaca actacaccag atgtaacatt acttggaata ttgagtcttc tgatgtcgct
     2761 tgtactcaca ttagataaat taaccatctg gattcccaaa gctggacgcg tcacttttcc
     2821 gtttttttct aactgttcaa taatattgat agcatcattt gcaggaattg cgaaaccaag
     2881 accttctaca gatgttcctc catttgtagc aattttactt gaggtaattc cgataacctg
     2941 cccttgaata ttgatcagtg ggccgccaga gttacctggg ttaatagcag tatcagtttg
     3001 gatggctttt gtagaaatag cttgtccatc ttccgatttt aaggatacat ttctattgag
     3061 actggatacg ataccttgag tgactgtatt tgcatattca gaacctaacg ggctaccgat
     3121 ggcaatagca gtttctccta cagttaactt actagaatca ccaaactcag ctactgttgt
     3181 cactttttct gaagagattt ttacgacagc aatatcagag aaagtgtcag ctccgacaat
     3241 ttctccaggt actttagtcc catctgacaa tcgaatatct actttgctgg cgccatttat
     3301 aacgtgattg ttggtgacga tgtaagcttc tttatcattc tttttataaa taactccaga
     3361 tccttcacta gagattcgct gagaatctgt gtcagtatca tcattgccaa atacgctatt
     3421 ttgtctgttt gccgaataag taataacaga aacaacagca tcttttactt tgttaacagc
     3481 ctgtgttgtt gaattttcgt tcttataggc agtttgtgta atagtactat tgttgttaga
     3541 gttgtttaca ctactttttt gagttagttg agttattgaa aaactaccca aggctccact
     3601 aaaaaagcta atgacgataa cgactaataa ttgaaaccat tttttgtaaa atgtttttag
     3661 atgtttcata tttgcctcca tatgtttgaa ttactgaaag tataaactga ctagcttaat
     3721 tataacttaa acacaaaagt tttacacaaa ctgtggataa ctcttttgaa actgtgattt
     3781 tcttaattga aatctatttt ttattttgtg aataagatgt gaaaaaatag agaatatgtt
     3841 agaatagagt catgaaaatt aaagttgtaa cagttgggaa actgaaagaa aagtatttaa
     3901 aagatggtat cgcagagtat tcaaaacgaa tttctagatt tgctaagttt gaaatgattg
     3961 agttatcaga tgaaaaaaca ccagataagg ccagtgaatc agaaaatcaa aagattttag
     4021 aaatagaagg tcagagaatt ttatcaaaaa ttgctgaccg tgatttcgtt attgtgttag
     4081 ccattgaagg gaaaactttc ttctcagaag aatttagtaa gcagttagaa gaaacttcta
     4141 taaaaggatt ttctactctt acttttatta ttgggggaag tttaggattg tcatcatctg
     4201 taaaaaatag agccaatctt tctgtcagtt ttggtcgcct aaccttaccc catcagttaa
     4261 tgagactagt tcttgttgaa caaatctatc gcgcttttac gattcagcag ggattcccct
     4321 accataaata gaaaattgac ttttaattga atttttggta gaataattgt attaggtctc
     4381 atagctcagc tggatagagc attcgccttc taagcgaacg gtcgcaggtt cgaatcctgc
     4441 tgggatcaat ataatagcaa agctgggaat tttcccggct tttttcttaa aaaagtacac
     4501 tttgggagaa aaaaatgaca gttgagagaa ttttatctaa aacgaaattc cattttgtat
     4561 aatggttttt gtaagttagc ttacaagaaa aaacatttta ggagatttta ttatgaaaaa
     4621 cacagttaaa ttggaacagt ttgtagcttt gaaggaaaaa gacttacaaa agattaaagg
     4681 tggggagatg aggttgtcaa aattcttccg tgattttatt ttacaaagaa aaaagtaatg
     4741 aaataagggg aaagagtaat ggatttattt ggatttggga cggttattgt tcatttttta
     4801 attattagtc acagttacca ttttatttgt aaaggtcaaa taaatagaaa agaattattc
     4861 gtttttggtg cttatacatt actaacagaa atagtatttg attttccctt atatattcta
     4921 tatttagatg ggttagggat tgaaagattt ttatttcctt tgggattata ttcctatttt
     4981 cgatggatga aacagtatga gagggataga ggactattcc taagtttact actatctctt
     5041 ttatatgaga gcactcataa ctttctgtcc gtaactttct cctctataac aggagataat
     5101 tttgttttac aatatcattt cccattcttt ttcgttgtga cggtgttaac ctattttgtt
     5161 acattaaaaa tcatttacta tttccatttg gaactagcct attttgacga ggactacctt
     5221 tatcctttct tgaaaaaagt attttttgct ttactattgc tacatattgt atctttcgtt
     5281 tcagatatgg taagtacgat taaacatttg aatagttttg gaagtatttt gtcatctatt
     5341 gtctttatct ctctcctttt gaccttcttt gcaatgaatt ctcataaagt tcaaatggag
     5401 aaagagattg ctttgaagca gaagaaattt gaacagaaac atttacagaa ttacacagat
     5461 gaaattgttg gtctgtataa tgaaatccgt ggttttcgac atgattatgc tggaatgctt
     5521 gtcagcatgc agatggcaat tgacagtggt aatttacagg aaattgacag aatttacaat
     5581 gaagttttag tcaaagcaaa tcataaattg cgttcagata agtacactta ctttgatttg
     5641 aacaacatag aagactcagc tttacgaagt ttggttgctc agtcaattgt ctatgctcga
     5701 aataatggtg tagagtttac actggaagta aaagatacga ttaccaagct tccaattgaa
     5761 ctattggatt tggttcgtat catgagcgtt ttattgaata atgctgtcga aggatcggct
     5821 gatagctata aaaagcagat ggaagtagca gttattaaga tggaaactga aacagttatt
     5881 gtgattcaga attcatgtaa aatgacgatg actccttcag gagatctatt tgccttagga
     5941 ttctccacta agggaagaaa tcgcggagtc ggattaaata atgtgaaaga actactagat
     6001 aagtacaaca atattatttt agaaacagag atggaaggca gtacatttag acaaatcatt
     6061 agatttaaga gggaatttga atgaaagttt taattttaga agatgttatt gaacatcaag
     6121 tgagactaga gagaatattg gatgaaattt cgaaagaatc gaatattcca atatcataca
     6181 agacaacggg aaaagtccgt gaatttgaag aatacattga aaatgatgaa gtaaatcagc
     6241 tttatttcct agatatcgat attcatggaa ttgagaaaaa gggatttgaa gtggctcagc
     6301 tcattcgtca ttacaatcct tacgctatta tcgtctttat cactagtcga tcagagtttg
     6361 cgactctaac ctataaatac caggtatcag ccctagattt tgttgataag gatatcaatg
     6421 atgagatgtt taagaagaga attgagcaaa atatcttcta cacgaagagt atgttacttg
     6481 aaaatgaaga tgttgtagat tatttcgact acaattacaa gggaaatgat ttaaaaattc
     6541 cttaccatga tattttgtat attgaaacaa caggggtctc tcataaattg cgcattattg
     6601 gtaagaattt tgcaaaagag ttttatggta ccatgacaga tattcaggaa aaggacaaac
     6661 atactcagcg attttattct cctcacaagt catttttggt aaatataggc aatatcagag
     6721 aaattgatcg aaaaaactta gaaattgttt tctatgaaga ccatcgttgt cctatttcaa
     6781 gattaaaaat tagaaaatta aaagatattt tagagaaaaa atctcaaaag tgattgacaa
     6841 ttagcaagaa attgatataa tggttatatc tgctacagat agaaggtccg ttggtcaagg
     6901 ggttaagaca ccgccttttc acggcggtaa cacgggttcg aatcccgtac ggactatttt
     6961 atccgccata gctcagttgg tagtagcgca tgactgttaa tcatgatgtc gtaggttcga
     7021 gtcctactgg cggagtatag ataaaaggga acacagctgt gttcctcttt ttgtatcaat
     7081 ttgtatcacc aagcattttc ataaggaagt ctgttatttc ttgagaactt tctttttttc
     7141 catgtgcaat ccaagtttgg cagacaccaa aaagtgcatg agttagatag atgctactat
     7201 attctaattc ggtggtattt agattcagtt gcataaatcg cttttgtaaa tctgtactaa
     7261 gcatgatatg aagtttattt cgtaagaaat tttggatttc tttagtccca ttttcagaaa
     7321 gaagggcagc cagaagtggt tctgactcta gatattcaaa aacttctaaa atagcgtctc
     7381 ttttgtgatg agcatgtttt tgaaaaatat attcaaatgt atggaatagc ttgctttgat
     7441 agtgctcaat catatcatac ttatccttat agtgagtata gaagctggaa cgactaattc
     7501 cggctttttc tactaatttg acagtagaaa ttttatcaaa tggctgttcc atcagtaatt
     7561 gtaccatagc attttcaata gttcgctttg tttttaagcg tttgttactt tcttgcatat
     7621 ttcctccttg taaacaaatt agactatatg tctaaaaata gattttttat cttgtaattt
     7681 agatttttta atgtataatc tattatatca aaattttaga caatatgttt aaaaaaggag
     7741 aaactaagtt taaagaatgg aaagcaattt aaaaaaaacc aacctttatt attgtcatga
     7801 tcgggatttc tcttattcca gatctgtaca atatcatatt tttgtcatca atgtgggatc
     7861 catatgggca attgtctgac ttacctgtgg cagttgtaaa taatgataaa gaggcttcct
     7921 ataatggtaa tactatggca ataggaaaag acatggtgtc caatttaaaa gaaaataaaa
     7981 ccttggattt tcattttgta gatgaagagg aaggaaagaa gggattggaa gatggcgatt
     8041 actatatggt agtgacttta ccaagtgatt tatctgaaaa aacaactaca ttatccaata
     8101 ttcaatcgac agcagcttat caatcattga caagtgagca acaaactgag ataagtgatt
     8161 ctgtatctca aaattcaact gatagtattc aatcggctca gtcaattgta gctttagtac
     8221 aagatttaca gggaagttta gaaaacttac aaaatcaatc ttctaatctt tcgactttaa
     8281 aaaatcaatc taatcaagta tcacctatta cttctacttc tttgatagga ttgtcaagtg
     8341 gattaacaga gatacaagga gatgttacta gcaaattagt tcctgccagt cagtcgattg
     8401 catcaggtgt aaacgcatat actacaggtg ttgataaagt ttctcagggc gcaagtcaac
     8461 taagtgaaaa aaatgccacc ttgacaggta gtttgaataa actagtttca ggctcaaaca
     8521 ccttgacaca aaaatcttct agattgacag caggagttgg ttaattacaa tcaggatctg
     8581 ggcaattagc agacaaatcc agtcagttac tttcaggtgc ttctccatta gagaatagag
     8641 ctaataaatt ggcagatgga tctgggaaac tagcagaagg tggaacaaag ttaacttctg
     8701 gattggaaga tttacagaca ggacttgctt ctttaggaca aggactaggt aatgctagtg
     8761 atcaactcaa atcagtatca acagaatcta aaaatgcaga gattttgtca aatccactca
     8821 atctttcaaa aacagacaat gatcaagttc ctgtaaatgg aatcgcaata gctccttata
     8881 tgatatcagt tgctcttttt tttgcagcaa tatcaacaaa tatgatattt gcgaaattgc
     8941 cttcaggacg tcatccagag agccgttggg cttggttgaa atcttgagct gaaataaatg
     9001 gtattatagc tgttttggca ggaattttgg tatatggagg agttcagctt attggtttaa
     9061 ctgctaatca tgagatgaga atatttattc tcatcatcct aacaagttta gtattcatgt
     9121 ctatggtgac cactttagca acgtggaata gccgtatagg agcttttttc tcacttattt
     9181 tgcttttact acagttagca tcaagtgcag gtacttatcc acttgctttg acaaatgatt
     9241 tctttagatc tattaatccc tggttaccaa tgagctattc agtttcggga ttacgacaaa
     9301 caatctctat caacaagtca ttttcctagc tgtcatacta gctctattta ctggtttagg
     9361 tatgctagcc tatcaacata agaaaatgga agaagattaa aaaaatcgac cgattaactg
     9421 gtcgattttt tatgccttag atgactttcg tctgtgatta tagattccaa atagtaagag
     9481 agaagtaaag gaacagattg ctccagtaat aaaaccattg ggaatgaagg aaagtgtaat
     9541 agttcctttc cccttgggaa tgtcaacttt cataaatcca gtttgagctt gtttaatttc
     9601 tattttctta ccatcttggt aggcagacca acctttgtca taaggaatgg tgaagaaaat
     9661 agatgtatct tgttggacat catatgtagc aaaaaccttg tttttagaag ttgatactgt
     9721 gacaggttgt tctttaattt tttgaattgc ctcggtgaaa gttttggtat ctaaacgata
     9781 gaaggtagga gattcaaatg atacttgtga atttccaggg aaactaacat tgatattgaa
     9841 agtttttttc tctttagtat atcctagatt aaagaaggag aagacattat cagttgtaaa
     9901 agtctttttt tcaccattta caaggatgtc aaccttcttt tgtttatcgt tagaaaagtg
     9961 aaggtttatg aaagagagat aaacttggct gttttctgga acttcaattt gatactggat
    10021 tgctgcatct tcatttgaag aacttgtgac actaatcaaa tcattagtat tttctatttt
    10081 ttctgttttt tcataaggta ttggagaaaa ataatcaaaa ttgacgttag caagttgatt
    10141 taaaaatgag gcctgattat ccaaggtatg ttcattgaac ttgacatcat tgtaaacaga
    10201 ttgactagca actgcaatcg gaagagagta ttgattttca tatagggtaa gattatcttt
    10261 ttgatagata tctttaaagc catacttatc aataggactg tctgagatat tgtactggat
    10321 accaaataaa ctatcagcca aaatactatt atttgcatat cggagattga gattagtccc
    10381 agaggattta aaaccaagtt tatctaaagt agagcttgat gaacgatttc gaacagatga
    10441 aaattgagag attccattgt agttgaattt catactgtca tttcctgtct gagtttgtag
    10501 tttttcagta cgagtaaatt gatttccaat atatgttgag aaagattcca tagctgggat
    10561 atctcgacta taagcacttc gagaagcaaa tccccattcc ttagcaattc cgtccatttg
    10621 agatgaagca tttaaactca tttcaaccag tataaataaa gagattagaa tggcaaatag
    10681 attcacagat ataaactttt tgataactgc aaggagtaaa agagaataga caaccaaaaa
    10741 ttcaagagta agcagaatat tcaaatctgt taaaaaagaa taatgcgatt ttagatagat
    10801 ggtagctaaa aatcctgtta ctacaagaaa aagcgaaact aaaaaattcc agactttaag
    10861 ttctttcaga cgctttaaga cttctgctgc tgtgtaaatt aacaaggtag agaaaatcca
    10921 agcatagcga tgtaaaaaca tgtttggagt atgcatgcct tgccaaaata agtcaagagc
    10981 ttctatgtaa aagcttgcaa ttagaaatgc aaagaatatt atatatatga gtttcacgtg
    11041 aaacttaata gatttcagcg taaaaaataa aatggtcaaa ataaagggaa gtagtccaac
    11101 aaaaatcatt gggatggccc catactttgt tgtgtcaaag gaaccaatga attgcttagc
    11161 aaagagatca agataccagc tactttcagt ttgaaacttt gtaacttcag tcaatttttc
    11221 cccatgtgtc tgtaaatcaa acagagtggg aagagtcata atcaaactag ccataccagc
    11281 taaaaaggag ataactatga aatcaagaac agatgatttt cgagtcttaa agtcccacga
    11341 aatttgacag agataccaga aaataagaaa caatactgtc atatatccaa aataataatt
    11401 ttgaataaat aagattgaca gacttgtaaa gtacaatagg agtttctttt cagttatcag
    11461 tagatgtaaa ccagttataa ttaaaggaat caagataaaa acatctagcc aggtttttat
    11521 ctctaattga ctgacagtga aactcatcag agcataggaa gtagataagg ctagctttaa
    11581 agtctgaggg atagattgaa acaatttatt caaactaaaa aaggttgaca gaccaatcaa
    11641 tccaaatttt aagagagtgg tcagatagac agcatctggc atattcgtta gatcaaaaaa
    11701 gtaaactaga ggcgcgagaa aactacccaa gtaataacta gatagggcat agaagtttag
    11761 ccctagacca cttgtaaagg tgtaaaacag actactattt ccatgtagga tatttcgtaa
    11821 ggctacatca aaaataacgt attgatgaaa gccatctcct aatagaggag agttgtcgct
    11881 attccagtag ataccttgag atagatatac tccagacata atcactacag gaatgatgaa
    11941 agaaataaaa taggttcgat atgtttttaa aaatgatttc atgttacctc gtagaatgat
    12001 agaaaactca gttggttaac ccaactgagt tttgaagttt tatttagtct ttccaaagtt
    12061 ctttaacttt tgcttgtact tctgcatttt ctaggaattc atcgtaggtt tcatcgatac
    12121 ggtcaatgac gccattttta gataagacaa tgatatggtt agccaaagtt tgaataaatt
    12181 cgtggtcatg gctagcaaag atgattgatt ctttaaagtt tttcaatcca tcattcaagc
    12241 ttgagataga ttccaagtcc aagtgatttg ttggatcatc aagtacaagg acatttgatt
    12301 ttaagagcat gagttttgaa agcatgacac gaactttttc tccccctgac aagacattta
    12361 caggtttgtt aacttcatct ccagagaaga gcatacggcc gaggaaacca cgtaggaaag
    12421 tattgtcatc ttcttcttta cttgcgaatt gacgcaacca gtcaagaatt gattctcctc
    12481 ctgcaaaatc agctgagtta tcttttggta ggtaagattg actagttgta actccccact
    12541 tgacagttcc ttcatagtca atatctccca tgattgcacg aattaatgca gttgtttgaa
    12601 tatcattttg tccaataagt gctgtcttat catctggacg caagatgaaa ctaatattat
    12661 ccaagatagt ttcaccatca atctttacag ttagattttc tactgtcaag agatcattac
    12721 caatctcacg ttccgcttta aagttgataa atggatattt acgactagat ggtacaatct
    12781 cttctagctc aatcttatca agcattttct tacgtgatgt tgcctgcctt gacttagaag
    12841 cattggcaga gaaacgagca acgaattctt gcaattgttt aattttttct tctgctttag
    12901 cattacggtc tgctagcaat ttagcagcaa gctcagaaga ttccttccag aagtcgtagt
    12961 ttccgacata gagtttgatt tttccaaagt caaggtcggc catgtgagta caaactttgt
    13021 ttaagaagtg acggtcgtgg gatactacga taactgtgtt atcaaagtca atcaagaagt
    13081 cttctaacca agtaatcgat tggatatcca aaccgttagt aggctcgtcc aagagaagaa
    13141 catctggttt accaaaaagt gctttggcga ggagaacctt tactttttca ccgttggcca
    13201 attcgctcat gttttggtag tgtaattctt ctggaatgtt taggttttga agtagttgag
    13261 aggcttcact ctctgcttcc caacctccaa gttcggcaaa ctctccttcg agttcggcag
    13321 cacgaacccc gtcctcgtct gagaaatctt ccttcatgta gatagcatct ttctctttca
    13381 tgatgctata aagtttttca tttcccatga taacgacatc aatggcacgt tcatcttcgt
    13441 agtcaaagtg attttgacga agaacagaga gacgttcatc tggaccaaga gagatgtgac
    13501 cagtagtagg ttcgatatct ccagctaaaa tttttaaaaa ggttgatttt ccggcaccat
    13561 tagcaccgat taatccgtaa gtatttcctt ctgtaaattt gatattgaca tcatcaaaaa
    13621 gtttgcgatc actaaaacgt agtgaaacat cagatactgt aagcaatatt tttctcctat
    13681 atgtgtaata tatttattct actagaaaat acagaaatat tcaaattttt atttgtcaat
    13741 tttgtgtaaa ttatatttac agtatccttt acacaaatct gtaaaaagca aggctgattt
    13801 attttgataa attacggtta tttcattaaa aaaatgctat aattgaaagg actatatcga
    13861 aggagaacaa aatgactaaa cccattattt taacaggaga ccgtccaaca ggaaaattgc
    13921 atattggaca ttatgttgga agtctcaaaa atcgagtatt attacaggaa gaggataagt
    13981 atgatatgtt tgtgttcttg gctgaccaac aagccttgac agatcatgcc aaagatcctc
    14041 aaaccattgt agagtctatc ggaaatgtgg ctttggatta tcttgcagtt ggattggatc
    14101 caaataagtc aactattttt attcaaagcc agattccaga gttggctgag ttgtctatgt
    14161 attatatgaa tctagtttcg ttagcacgtt tggagcgaaa tccaacagtc aagacagaga
    14221 tttctcagaa aggatttgga gaaagcattc cgacaggatt cttggtctat ccaatcgctc
    14281 aagcagctga tatcacagct ttcaaggcta attatgttcc tgttgggaca gatcagaaac
    14341 caatgattga gcaaactcgt gaaattgttc gttcttttaa caatgcatat aactgtgatg
    14401 tcttggtaga gccggaaggt atttatccag aaaatgagag agcagggcgt ttgcctggtt
    14461 tagatggaaa tgctaaaatg tctaaatcac taaataatgg tatttattta gctgatgatg
    14521 cggatacttt gcgtaaaaaa gtaatgagta tgtatacaga tccagatcat atccgcgttg
    14581 aggatccagg taagattgag ggaaatatgg ttttccatta tctagatgtt tttggtcgtc
    14641 cagaagatgc tcaagaaatt gctgatatga aagaacgtta tcaacgaggt ggtcttggtg
    14701 atgtgaagac caagcgttat ctacttgaaa tattagaacg tgaactgggt cctattcgtg
    14761 agcgccgtat tgaatttgct aaggatatgg gagaagttta taatatgatt caaaaaggta
    14821 gtgaaagagc gcgtgaagtt gcgggtcaaa ccctatctga ggtaaaaggg gcaatgggac
    14881 ttcattactt taactaagtt tattttacaa gaaactatca tttctatctc gaagaaaaga
    14941 tagtttttta tttacccctg taacatttga caaattttta tagaatggta tgatagatac
    15001 aatataaaaa gagtcaagct caaaaagaaa gaaaagagga aacttcgaat gtctaattgg
    15061 gacactaaat ttttgaaaaa aggttttacc tttgatgatg tattgcttat tccagctgaa
    15121 agtcatgtgt tgcctaacga tgcagattta acaactaaat tggcagataa tttgacttta
    15181 aatatcccaa ttattaccgc tgccatggac acagttacag agagtcaaat ggccattgct
    15241 attgctcgtg caggtggtct cggagttatc cataaaaaca tgtcaattgc tcaacaagca
    15301 gacgaggttc gtaaggtaaa acgttctgaa aatggagtta ttattgatcc gttcttcttg
    15361 acgcctgaac atacaattgc tgaagcagat gagcttatgg gtcgttaccg catcagtggt
    15421 gttccagttg ttgaaacact tgaaaatcgt aaattggttg gtattttgac aaaccgagat
    15481 cttcgtttta tttcagatta taatcaacca atttcaaacc atatgactag tgaaaatctt
    15541 gttactgctc ctgtgggtac ggatcttgca acggctgaga gtattcttca agagcatcgt
    15601 attgaaaaac ttccgttggt cgatgaagaa ggcagtcttt ctggtttgat cactatcaaa
    15661 gatattgaaa aagttattga gtttccaaat gcggctaaag atgagtttgg tcgtcttcta
    15721 gttgcaggtg cagtaggtgt tacttcagat acatttgaac gtgcagaggc tctttttgag
    15781 gcaggagcgg atgcgattgt tattgatact gcacatggtc attctgcagg tgtcttgcgt
    15841 aaaattgccg agattcgtgc tcatttccca gatcggactt tgattgctgg aaatattgct
    15901 actgctgaag gtgcacgtgc cctttatgaa gcgggtgtag acgttgttaa ggttggtatt
    15961 ggaccaggtt ctatctgtac tactcgtgtg attgctggtg ttggtgttcc gcaagtaaca
    16021 gctatctacg atgctgcagc tgttgcgcgc gaatatggta aaacgattat tgctgacggt
    16081 gggatcaagt attctggaga tattgtaaaa gcacttgctg caggtggaaa tgctgttatg
    16141 cttggatcta tgtttgctgg aactgatgaa gctccaggcg aaactgaaat cttccaagga
    16201 cgtaaattca agacttaccg tggtatggga tcaattgctg ctatgaagaa aggttcaagc
    16261 gaccgttatt tccaaggttc tgtcaatgaa gcaaacaagc ttgttccaga aggaattgaa
    16321 ggtcgtgttg cttataaagg agcggcagct gatattgttt tccaaatgat tggtggtatt
    16381 cgctctggta tgggttactg tggtgcagct aaccttaaag aactacacga taatgctcaa
    16441 tttattgaaa tgtctggtgc tggtttgaaa gaaagccatc ctcatgatgt gcaaattact
    16501 aatgaggcac caaattattc tatgtaaaaa acaatgaaaa gaactccagt gaaaacagga
    16561 gttcttttac aatgttgtca atttccattt acagcagctt taccatcctg aatagtgaag
    16621 atacttagat tttctggcag attttgaaga tggtctaagc ttgttgttgt gataaaggtt
    16681 tggattgatt gagaaatcgt ttctaataat tttaactgtc tagtgttgtc aagttcactc
    16741 atcacatcgt caagcagtaa tatcggagat tctgtggtaa tgctttccat taattcgatt
    16801 tctgctaatt ttatcgagag gacgagacta cgatgttgac cttggcttcc gaaactagca
    16861 tccatcccat ttatataaaa agaaatgtca tctcgatgag gaccgacacc agtattcttt
    16921 ttaaataaat ctctggatct acttttttct aaagcaattt tgaaagattc ggataagttt
    16981 tgtttgtcag ttgtattgac agaagattga taggatattg acaactcttc gatctgatta
    17041 gagagttcaa aatgtttctt acgcccaaat gattctagtt tttttatgaa atctaagcgg
    17101 tgattcatta cacgacatcc ataatcaact agctgatcat ctaacacaga aaggaatgtt
    17161 tcatctattt tttgaactga ttttaggtaa gtgtttcttt gctttaggat gtggttataa
    17221 ttggttaagt cagataaata gattggctta atttgcccaa gctccatatc aatgaatttt
    17281 cgtcgaatcg aaggtgctcc tttaattagt tgtaaatctt caggagcaaa taagacaaca
    17341 ttcatgtgtc ctacataatc tgaaaggcgt gcctgtttta agtgattaac ttttgttaca
    17401 cggccttttt gtgttagttc gatttctagg ggaatggatc cagttttttt ctgaacgaga
    17461 cctgaaagat gaagttgttc ctcatcaaaa tgaatgagat ttttatctgt tcgagttcga
    17521 tgactacgcg ttaaggctaa aaaatagata gcctctaaca tgtttgtttt tccttgtgca
    17581 ttacgtccca aaaagacatt caatttagga ttaaagtcta ttttcgtttc tttgtaatta
    17641 cgaaaagtct tgagagatag atgttgtagc cacatgatta tctaccaggg aatcgtggag
    17701 cttgttggct tttaggtgat gaagcaggtt tcgatttgtc ttttttaact cccttattca
    17761 tctttttgac aagtttagcg atccgttctt tttcaacttt atcagcttgg tattcatctt
    17821 gttcttcaga agtaggttgt gtcaacaaga tgtcaatgtt catgtcaggg atgtcaactt
    17881 tatcgccaat acgaagtttt tttccacgac gactctctaa ttccctatta aagtaaacag
    17941 aatgttcaga gagaaatgat ttaatagctc ctccgctatg tgtaattcca agttctttga
    18001 gtagtgcttg gagggtaata aattcttcaa ataatttgta ttccataatt cacctcaatc
    18061 tttggtatta taccatattt tcctaaaaat agtgagagag atagctagaa atgtaagcga
    18121 tttttatttc aaaagtgtta cagggaacaa gaaaatttca ggttttcgtg atataataga
    18181 agtctgtata taaggaggta aatcatggag ttagtgcatg gaatttcaac acattttatc
    18241 caatcaaaaa agtttaaaac aaacaaaatt accgtgcgtt ttaccgctcc attatccctt
    18301 gatacgattg caggtcacat gttgagtgca agtatgctag agactgctaa tcagatgtac
    18361 cccacttctc aagatttgag gagacacttg gccagtctat acggtacaga tatgtcaacc
    18421 aattgtttca gaagagggca aagccacatt atagaattga catttaccta tgttcgtgat
    18481 gagtttttaa gtaggaaaaa cgtgctaacc tctcagattt tggaacttgt aaaagaaact
    18541 cttttttcac ccgcagtagt tgataatggg tttgatccgg ccttatttga aattgagaaa
    18601 aaacaattgc tagcaagttt agcagctgat atggatgatt ctttttattt tgcacataaa
    18661 gaattggata aattgttttt tcatgatgaa cgtcttcaat tggaatatag tgatttacga
    18721 aatcgtattt tagctgaaac tccacaaagt tcttattctt gtttccaaga atttttagcc
    18781 aatgatcgaa tagatttctt tttcctaggt gattttaatg aggttgaaat tcaaaatgta
    18841 ttagaatcat ttggctttaa aggtcgaaaa ggagatgtga aggttcagta ttgtcaacct
    18901 tattctaata tccttcagga aggtatggtt cggaaaaatg tgggacaatc cattttggaa
    18961 ttaggttatc attaccgttc taaatatggt gatgaacaac atttacccat gattgtaatg
    19021 aatggtttac ttggtggatt tgctcactct aagctcttta caaatgtccg tgaaaatgct
    19081 ggattagctt ataccatttc aagtgagctt gatttattta gtggattctt gaggatgtat
    19141 gctggtatca atcgagaaaa tcgtaaccag gctcgtaaaa tgatgaataa tcaactgctt
    19201 gatttaaaaa aaggttattt tacagagttt gagttaaatc agaccaagga aatgattcgt
    19261 tggtcgttgt tactttctca agataatcaa tcttcattga ttgaacgtgc ttatcaaaat
    19321 gccttatttg gaaaatcttc agcagacttt aaaggttgga ttgcaaaact cgagcaagtt
    19381 gacaaagatg ctatttgtag agcagctaat aatgtgaaac tacaggcgat ttactttatg
    19441 gaaggaatag aatgacaaag gttgtttttg aaaaaaaata ctatccagct gtaaaagaaa
    19501 tgatttatcg aactcgtttg gccaacggat tgacagttgc tcttttgcct aaaaaggaat
    19561 ttaaagaggt ttacgggagt gtcactgtac agtttggttc ggtagatacg tttgtcacag
    19621 aagttgacgg agatgtaaaa caatatcctg caggaattgc tcattttctt gaacataaat
    19681 tatttgagag agaagattct agtgatttga tgtcggcttt tacgagtcta ggtgcagata
    19741 gtaatgcctt tacaagcttt acaaaaacaa actatctttt ttcagcaacg gattattttt
    19801 tagaaaattt atatttactt gatgaattgg taacatcagc acactttact gaagcttcca
    19861 ttctgacaga gcaggatatt attcagcaag aacgagaaat gtaccaagat gatccagatt
    19921 cgtgtttatt cttttcaact ttagcgaatt tgtatcctgg tacaccttta gcaactgata
    19981 tagttggaag tgaggagtcc atttcccaaa tcaatctaac taatttgcaa gaaaatttta
    20041 caaagtttta caaacctgta aacatgtctc tgtttttagt tggtaatttt gatgtggagc
    20101 gagtacagga ctattttgaa agcaaagaac tgaaagattc agattttcag gaagtagcaa
    20161 gagaaaagtt gtttttacag cctgtaaagc caacagatag tatgagaatg gaagtatctt
    20221 ctcccaaact agcgattgga gttagaggta agcgagaagt ttctgaagcg gattgctatc
    20281 gacatcatat tttattaaaa ttattgtttg caatgatgtt tagttggact tcggatcgtt
    20341 ttcaaaaatg ttatgaatca ggtaaaattg atgcatcctt atctctggaa gttgaagtaa
    20401 caagtcgctt tcattttgtc atgttgacaa tggatacgaa agagccagtt gctttgtctc
    20461 atcaatttag gaaggctatt cgtaatttta caaaggattt agatattaca gaggaacatt
    20521 tagatattat caaaagagag atgtttggcg aatttttcag tagcatgaac tctcttgaat
    20581 ttattgcaac gcaatatgat gcttttgaaa atggtgagac aatttttgat ttgccgaaaa
    20641 ttttacagga aattacttta gaggatgtcc ttgatgctgg acatcattta atagatgatg
    20701 gtgacatagt tgattttaca atattcccat cgtagtaacc tattataata gacactagaa
    20761 agaagggatg acaagtatga gaaaaaaaac aattggagag gttttacgat tagctagaat
    20821 caatcaggga ttgagtttag atgaattgca gaaaaagaca gaaatccagt tagatatgtt
    20881 ggaagcaatg gaagcagacg atttcgatca acttccaagt cctttttaca cgcgttcttt
    20941 cttgaaaaaa tatgcatggg ctgttgagtt agatgaccaa attgttttgg atgcttatga
    21001 ttctgggagt atgattactt atgaggaagt agatgttgat gaagatgagt tgacaggtcg
    21061 tagacgttca agtaagaaaa agaagaaaaa aacatcattt ttacctttat tttattttat
    21121 cctttttgct ttatcgattt taatttttgt gacttattat gtttggaact atattcaaac
    21181 tcaaccagag gagccttctc tttctaatta cagtgtggtt caatcaacaa gttcaactag
    21241 ctctgttccc cactcctcaa gtagtagttc ttctagtata gaatcagcta taagtgtatc
    21301 aggcgaagga aatcatgtag aaatcgctta taagacaagt aaggaaacag ttaaattgca
    21361 attggcagtt tcagatgtta caagttgggt cagtgtttca gaaagcgaac ttgagggcgg
    21421 tgtaacctta tcgccaaaga agaaaagtgc agaagcaaca gttgcaacta aaagtcctgt
    21481 aacaattacg ttaggtgttg taaaaggtgt tgatttgaca gtagataatc agactgttga
    21541 tttatcgaaa ttaacagctc agactggaca aatcactgta acctttacta aaaattaagg
    21601 aaaaacgaat gaaaaaagaa caaattccca atctcttaac aataggtcga attctcttta
    21661 tacctatttt tatctttatt ttaacgatag gaaattcgat agagagtcat atagttgcag
    21721 ctattatctt tgctgttgcc agtattaccg actatttaga tggatattta gctcgtaaat
    21781 ggaatgtggt cagtaatttt ggtaaatttg cagatcctat ggcggataag ttactagtta
    21841 tgtcggcttt tattatgttg attgagttag gtatggctcc ggcttggatt gttgcagtga
    21901 ttatctgtcg tgagttagct gtgacaggtt taaggctttt attggttgaa actggtggaa
    21961 caattttagc agcagcaatg cctggaaaaa ttaaaacttt tagtcagatg tttgccatta
    22021 ttttcttgct attacattgg actttgcttg gtcaagttct actttatgta gccttatttt
    22081 tcactatcta ctctggctat gactatttca agggtagtgc ctatgtattt aaagggacat
    22141 ttggttcgaa atgaaatcaa taattgatgt aaaaaatctt tcttttcgct ataaagaaaa
    22201 tcagaactac tacgatgtga aggatattac gtttcacgtg aaacgtggag aatggctttc
    22261 gattgtaggg cataatggta gtggtaaatc aacgacggtt cgattaattg atggcttact
    22321 ggaagcagaa tccggagaga ttgtaattga tggccaacgt ttgactgagg aaaatgtttg
    22381 gaatatacgt cgtcaaatcg gtatggtttt tcaaaatcca gacaatcaat ttgttggagc
    22441 gactgttgaa gatgatgttg cttttggttt ggaaaatcag ggactttctc gtcaagaaat
    22501 gaaaaagaga gtggaagaag ctctggcttt agttggcatg ttggacttta aaaagagaga
    22561 gccagcgcgt ctatcaggtg gtcaaaagca acgtgtggcc attgcaggtg ttgtagccct
    22621 aagaccagct attttaatct tagatgaagc aacgagtatg ttggatcctg aggggcgtag
    22681 agaacttatt gggacagtaa aaggaattcg aaaagactat gatatgacgg tcatttctat
    22741 tacccatgat ttggaagaag tcgccatgag tgatcgcgta ttggtcatga aaaaagggga
    22801 aattgaatca actagtagtc caagggagct tttctctcga aatgatttag atcaaattgg
    22861 attagacgat ccttttgcta atcaattaaa aaaatctttg agccagaatg gctatgattt
    22921 acctgaaaat tatttgacag aaagtgagct agaggataag ctatgggaat tgctctagaa
    22981 aatgtgaatt ttacatatca agaaggtact cccttagctt cagcagcttt gtcggatgtt
    23041 tctttgacga ttgaagatgg ctcttataca gctttaattg ggcacacagg tagtggtaaa
    23101 tcaactattt tacaactctt aaatggttta ttggtgccaa gtcaagggag tgtgagggtt
    23161 tttgatacct taatcacctc gacttctaaa aataaagata ttcgtcaaat tagaaaacag
    23221 gttggcttgg tatttcagtt tgctgaaaat cagatttttg aagaaacggt tttgaaggac
    23281 gttgcttttg gaccgcaaaa ttttggagtt tctgaagaag atgctgtgaa gactgcgcgt
    23341 gagaaactgg ctctggttgg aattgatgaa tcactttttg atcgtagtcc gtttgagctg
    23401 tcagggggac aaatgagacg tgttgccatt gcaggcatac ttgccatgga gccagctata
    23461 ttagtcttag atgagccaac agctggtcta gatcctctag ggagaaaaga gttgatgacc
    23521 ctgttcaaaa aactccacca gtcagggatg accatcgtct tggtaacgca tttgatggat
    23581 gatgttgctg aatatgcgaa tcaagtctat gtaatggaaa agggacgttt agtaaagggg
    23641 ggcaaaccaa gtgatgtctt tcaagacgtt gtttttatgg aagaagttca gttgggagta
    23701 cctaaaatta cggccttttg taaacgattg gctgatagag gcgtgtcatt taaacgatta
    23761 ccgattaaga tagaggagtt caaggagtcg ctaaatggat agtatgattt tggggcgtta
    23821 tatcccaggg gattcgattg ttcaccgatt ggatccacgt agcaaattgc ttgctatgat
    23881 gctactgatt ttgattgtgt tttgggctaa taaccccttg acgaatctga ttctttttat
    23941 agcgacaggg atatttattg ccttgtcagg agtatctctt tcatttttta ttcagggctt
    24001 gaagtctatg tttttcttga ttgccttcac aactattttt caactatttt tcatttctaa
    24061 tgggaatgtt ttatttgagt tttcgtttgt gagaatcacg gattatgctt tgcaacaagc
    24121 tgggattatt ttttgtcgct ttgtattgat tattttcttt tcaactttgt taaccttaac
    24181 aaccatgcct ttaagtttgg catcagctgt cgaagcttta ttagcacctt taaagcgtgt
    24241 gaaagttcca gttcatgaaa ttggattgat gctgtctatg agtttgcgtt ttgtcccaac
    24301 cttgatggat gatacgacgc ggattatgaa tgcacagaaa gctcgtggag tggattttgg
    24361 agaaggaagc atcgttcaaa aagtaaaggc gatgattccc attttgattc ctctttttgc
    24421 gacaagttta aaacgtgcag attccttggc tatcgctatg gaagcgcgtg gctatcaggg
    24481 tggaaaaggc agaagtcaat accgacaatt gaaatggact ctaaaggata cgttgaccat
    24541 tcttgttatt ctcgtacttg gttgttgttt atttttctta aaatcttagt agctttcagg
    24601 aattgataaa aagttactgt aacagttttt gatataaagg taggaatatg aaccgtttta
    24661 aaaaatcaaa atatgtcatt attgtttttg tcactgttct gcttgtgtca gctctcttag
    24721 cgacgactta ttcaagtaca attgtgacaa aattaggaga tggaatctca ttggttgata
    24781 gagttgtaca aaaacctttt cagtggtttg attctgtcaa atcagatttg gctcatttga
    24841 cacgaacata taatgaaaat gaaagtttga agaaacagct ttaccaatta gaagttaaat
    24901 caaatgaggt ggaaagttta aagacagaaa atgaacaact gcgccaattg cttgatatga
    24961 agtctaaatt gcaagccaca aagactttag cagcagatgt tattatgcgt tctccggtat
    25021 cttggaagca ggagttgacc ttagatgcag gtagatcaaa aggtgcttct gagaacatgt
    25081 tagctattgc aaatggtggc ttgattggga gtgtttcaaa agtagaggag aactctacta
    25141 tagtcaacct tctgacaaat acggaaaatg ctgataagat ttctgttaaa attcaacatg
    25201 gctctactac aatttatgga attattattg gctatgacaa ggaaaatgac gttcttaaaa
    25261 ttagccaatt aaatagtaat agcgatatta gtgcgggaga taaggtgact actggtggat
    25321 taggaaactt taacgttgct gatattcctg ttggtgaagt ggttgccaca acgcatagta
    25381 cagactattt gacacgagaa gtaactgtta aattgagtgc agatactcat aatgtagatg
    25441 tgatagaatt agtggggaat tcataatgag acagttgaag cgagttggag tatttttatt
    25501 gcttcctttc tttgttctaa ttgacgccca tattagccag cttctgggct catttttccc
    25561 ccatgtacat ttggctagtc attttctttt tctatttctc ttatttgaga cgatagaagt
    25621 atcagagtat ctctacctag tctattgttt tgttataggc ttggtttatg atgtttactt
    25681 tttccatcta atagggatta caactctctt atttatctta ttgggagcct tccttcataa
    25741 attgaatagt gttattttgt tgaatcgttg gacaagaatg ctagctatga ttgtgctgac
    25801 attcctgttt gaaatgggta gttatctttt ggcttttatg gtagggttga cagtagatag
    25861 catgtcgatt tttatagtct atagcttggt accgacgatg attttaaatt ttttatggat
    25921 tactgttttt caatttattt ttgaaaaata ttatctataa gaacgacata taaatgtaac
    25981 aaaggcgtaa tatttattag gccttttttt ggtatactag tattgtcttt aaaagaagga
    26041 gtatctacgt aatatgaaga aaaaaatctt agcgtcactt ttattaagta cagtaatggt
    26101 ttctcaagta gctgttttaa caactgcgca tgcagaaacg actgatgaca aaattgctgc
    26161 tcaagataat aaaattagta acttaacagc acaacaacaa gaagcccaaa aacaagttga
    26221 ccaaattcag gagcaagtat cagctattca agctgagcag tctaacttgc aagctgaaaa
    26281 tgatagatta caagcagaat ctaagaaact cgagggtgag attacagaac tttctaaaaa
    26341 cattgtttct cgtaaccaat cgttggaaaa acaagctcgt agtgctcaaa caaatggagc
    26401 cgtaactagc tatatcaata ccattgtaaa ctcaaaatca attacagaag ctatttcacg
    26461 tgttgctgca atgagtgaaa tcgtatctgc aaacaacaaa atgttagaac aacaaaaggc
    26521 agataaaaaa gctatttctg aaaaacaagt agcaaataat gatgctatca atactgtaat
    26581 tgctaatcaa caaaaattgg ctgatgatgc tcaagcattg actacgaaac aggcagaact
    26641 aaaagctgct gaattaagtc ttgctgctga gaaagcgaca gctgaagggg aaaaagcaag
    26701 tctattagag caaaaagcag cagctgaggc agaggctcgt gcagctgcgg tagcagaagc
    26761 agcttataaa gaaaaacgag ctagccaaca acaatcagta cttgcttcag caaacactaa
    26821 cttaacagct caagtgcaag cagtatctga atctgcagca gcacctgtcc gtgcaaaagt
    26881 tcgtccaaca tacagtacaa acgcttcaag ttatccaatt ggagaatgta catggggagt
    26941 aaaaacattg gcaccttggg ctggagacta ctggggtaat ggagcacagt gggctacaag
    27001 tgcagcagca gcaggtttcc gtacaggttc aacacctcaa gttggagcaa ttgcatgttg
    27061 gaatgatggt ggatatggtc acgtagcggt tgttacagct gttgaatcaa caacacgtat
    27121 ccaagtatca gaatcaaatt atgcaggtaa tcgtacaatt ggaaatcacc gtggatggtt
    27181 caatccaaca acaacttctg aaggttttgt tacatatatt tatgcagatt aatttacaga
    27241 gggactcgaa tagagccctc ttttcaggtt ttaccgtgac aatccctatt aaaaattata
    27301 tcaaaatagc ttgaaaatat tggaaaagta tggtaaaatg aaaattgtcg tgtgaacgat
    27361 aatactcatt cttgatgaat tgtgaagcag ttgcccttgg gtcgttttgc gagttgaagt
    27421 caagaagagg aaaaaaacaa aaaggagaaa tactcatggc agtaatttca atgaaacaac
    27481 ttcttgaggc tggtgtacac tttggtcacc aaactcgtcg ctggaatcct aagatggcta
    27541 agtacatctt tactgaacgt aacggaatcc acgttatcga cttgcaacaa actgtaaaat
    27601 acgctgacca agcatacgac ttcatgcgtg atgcagcagc taacgatgca gttgtattgt
    27661 tcgttggtac taagaaacaa gcagctgatg cagttgctga agaagcagta cgttcaggtc
    27721 aatacttcat caaccaccgt tggttgggtg gaactcttac aaactgggga acaatccaaa
    27781 aacgtatcgc tcgtttgaaa gaaattaaac gtatggaaga agatggaact ttcgaagttc
    27841 ttcctaagaa agaagttgca cttcttaaca aacaacgtgc gcgtcttgaa aaattcttgg
    27901 gtggtatcga agatatgcct cgtatcccag atgtgatgta cgtagttgac ccacataaag
    27961 agcaaatcgc tgttaaagaa gctaaaaaat tgggaatccc agttgtagcg atggttgaca
    28021 ccaatactga tccagatgat atcgatgtaa tcatcccagc taacgatgac gctatccgtg
    28081 ctgttaaatt gatcacagct aaattggctg acgctattat cgaaggacgt caaggtgagg
    28141 atgcagtagc agttgaagca gaatttgcag cttcagaaac tcaagcagat tcaattgaag
    28201 aaatcgttga agttgtagaa ggtgacaacg cttaatttat acaaatagta attacctagg
    28261 agggcggggc ttagcccggc tctcctattt tcaaaaaata taggagaatt aaaatggcag
    28321 aaattacagc taaacttgta aaagagttgc gtgaaaaatc tggtgccggt gttatggacg
    28381 ctaaaaaagc gcttgtagaa acagacggtg acatcgaaaa agcgattgaa ttgcttcgtg
    28441 aaaaaggtat ggctaaggca gctaagaaag ctgaccgtgt tgctgcagaa ggtttgactg
    28501 gtgtttatgt taacggtaat gttgcagcag ttattgaagt aaacgctgaa actgacttcg
    28561 ttgcaaaaaa cgctcaattc gttgaattgg taaatactac agctaaagtc attgctgaag
    28621 gaaaacctgc taataatgaa gaagctcttg ctttgataat gccttcaggt gaaactcttg
    28681 aagctgcata cgtatctgca acagcaacta tcggagagaa aatctcattc cgtcgctttg
    28741 cattgattga aaaaacagac gcacaacact ttggagcata ccaacataac ggtggacgta
    28801 tcggtgttat ttcagttgtt gaaggtggag acgaagcact tgctaaacaa ttgtcaatgc
    28861 acatcgcagc gatgaaacca acagttcttt cttacaaaga attggatgag caattcgtta
    28921 aagatgagtt ggcacaattg aatcacgtta tcgaccaaga caacgaaagc cgtgcaatgg
    28981 ttaataaacc agctcttcca cacttgaagt atggatcaaa agctcaatta actgatgatg
    29041 ttattgctca agctgaagct gacatcaaag ctgaattggc tgcagaaggc aaaccagaaa
    29101 aaatctggga caaaattatc ccaggtaaaa tggatcgctt catgcttgat aatactaaag
    29161 ttgaccaagc ttacacactt cttgcacaag tttacatcat ggatgacagc aagacagttg
    29221 aagcatacct tgaatcagtt aacgcttcgg tagttgagtt tgctcgcttt gaagttggtg
    29281 aaggtatcga gaaagctgca aacgacttcg aagctgaagt tgcagctaca atggcagcag
    29341 ccttgaataa ctaataatag aaaggaagca atttgcttcc ttttttgtat agggcttata
    29401 tttttcctga aattgttttt cccccagtct cactcgatta gaataattaa aacaaaaagc
    29461 cctataataa gggctaagtg catttaggag actatacttc aaattcatag taagcgcgtc
    29521 ataacaaggt atctatccta attccccaac ttccagtata ctagataaaa aataattatc
    29581 ggaggaaagg tattgtacta tgatgattca actaagtgat ttaggtcaag ttcacattgt
    29641 ttgtggcaag acagatatga ggcagggaat agactcatta gcctatgtag ttaaaaccca
    29701 ctttgaattg gattctttct ccggtcaagt ctttctcttt tgtggtggac gtaaagaccg
    29761 ctttaaagtc ctttactggg atggtcaagg attttggcta ctatataaac gctttgagaa
    29821 cggaaaactg acttggctaa gtacagaaaa ggatgtcaaa gctctcacat ctgaacaagt
    29881 agactggctt atgaagggct tttctatcac tccccaaata tagtggattt aaactagaat
    29941 agtacacctc tgcttctaaa acattgttag aaatcgattt gactatcctg ttcttatttc
    30001 attttactat aaattcatca gaaagtcgtg atttctattg aaatgaggac tttcttttta
    30061 tactcatctg ttttcaaaaa gcattctagt ccgtctccga ttaacgatgg actttatcac
    30121 ctccttctcc agtccttgta tgacatcttc caaagttcga aaggctttat tcttaaatcc
    30181 acgtttacga atctctttcc acacttgttc aatggggttc atctctggtg tgtatggagg
    30241 aataaatgca aagccaatat tagtcggaat ctttaaggta cttgatttat gccatatagc
    30301 attgtccata acgagtaaaa gataatcatc tggataagct tgtgaaagct cctattccta
    30361 aagccccttt agcgcataac tttggctcag cttctattat cgctcacacc atccatcaga
    30421 agtttaatct gaaggtaccc aattatcgcc aagaagaaga ttgggctagg atgggtttac
    30481 caatcacacg taaggaaatc tctaattggc atatcaagac gagtcaatac tatttggagc
    30541 ccctttataa cctcttgcga gagagactat tgactcagcc cttacttcat gcggatgaaa
    30601 cttcttatag ggtgctagag agtgatagtc agctgactta ctattggact tttttgtctg
    30661 ggaaagctga gaaacaaggg attacgcttt accatcatgt tctaatcgat ttgttcatat
    30721 cttatttcaa tccattataa atagtgagaa atatctatcc tatcttctag aatgtcttcc
    30781 aaacgtggaa actctcgtaa acaaagaggt tttagaggcc tatttaccat ggactaaagt
    30841 tgtacaagaa aagtgcaaat aagaaatctc cagattagga actatccgtg agttctctag
    30901 tctggagatt tttcaataga cttcgttatt ggacggttac aattcataaa gtgctgtaga
    30961 gagataacgt tcaccgttat ctggtgctag ggcaaggact tttttacctg tacctaattt
    31021 tttggcaacc tcgatggctc cgtagatagc tgcagctgag gaaatcccta caaggaagcc
    31081 ttcttttcca ccaatttcac gtccgagtgc aagagcgtca tctgatgtta cacgaacgat
    31141 accatcatag gctttagtat caagtgtatc aggaataaat ccagctgaga taccttgaat
    31201 tttgtgagga ccaggttttt caccagatag aatagcagat tcatctgctt ctactgcaaa
    31261 aacttgaatg ttagaatttt ctgatttgag tgcatgagaa acaccagaaa tcgttccacc
    31321 agtacctact ccagcaacaa aggcatctaa tccatcttta ccgaaagcag ctagtatctc
    31381 agctcctgtt gttctttcgt gtacttctgg attagctgga ttgtcaaatt gaagaggaag
    31441 gaaaccatca cgttcagcag cgatttcttg agccttagca atagcacctt tcattccctc
    31501 gctaccagga gttaggacga gttcagcacc ataagcttgg ataattttac gtcgttctac
    31561 actcatagtt tcaggcataa cgatgacgac tttataccct ttagcagcac ctacccatga
    31621 aagtccaata ccggtgtttc cacttgttgc ttcaacaata gtagaaccag gtttcagaat
    31681 accatcttgt tcagcttttt caatcatgct aagggcaata cggtctttta cagatgaacc
    31741 aggattaaat gcttcaagct ttatatagac gtctgcagca ccttctggca cgatgttgtt
    31801 aagtttaaca atcggtgttt gaccgattaa ttcagtaatg ttgttataaa tagacatatg
    31861 aatacctctt tgtataagat atctttagta taacgctatc cataccattt ctatatatca
    31921 tagatcaaag tcattaattt tcaagtgatt gcgttcagct aattctagta aataggtgtg
    31981 aaggaaaacg atatcaggtt tcgtttgaat tgcttcattt tctagtatat tttggtacgc
    32041 ctctttttat tttgctcgct aaagtagttg tgagaatgtc aacgaaacgc tgtattattc
    32101 attgtttaca ctaagtttac aggaacttca acctctcgta aaccttggtc agttaaagtg
    32161 acttttccat taaaaaactc cacaagtgca gctttaatag tttctttttc ttctttatca
    32221 acataaatca tcgtatcgac ttgatctgta aagtttgtat ccagctccat gagaccatgt
    32281 tctttaagga agttactgta ctcttggtac tgagcataag acatttgaat agcaatgcca
    32341 gcctgttctt ttatttcaat aataccaatt tctttgacag ctaaggcgac actgccggcg
    32401 taagcacgaa ttagtcctcc agcgcctagt ttaataccac caaagtagcg tgtcacgacc
    32461 acacagacat tggtgagatt gtgattttct agtaccccaa gcatgggaac accagcagta
    32521 ccactaggct caccatcatc acttgtacgt ttaatttcac tacgttctcc aataatgaag
    32581 gcagagcagt tatgtgtcgc tttgtagtgt tcttttttga tggcagtaat gaagtcacga
    32641 gcctcttctt cgctataaac acgcttggca tggcagataa agcgagattt tttgatttct
    32701 tcttggactt gaccgtcctc tttaattgtc ctaaattcca tgatttaatt gtactaaaaa
    32761 atcatctaaa gtgctagttt ttacgaataa agaagtatga aagtaaattt agattatctc
    32821 ggtcgtttat ttactgagaa tgaattaaca gaagaagaac gtcagttggc ggagaaactt
    32881 ccagcaatga gaaaggagaa ggggaaactt ttctgtcaac gctgtaatag tactattcta
    32941 gaagaatggt atttgcccat cggtgcttac tattgtcgag agtgcttgct gatgaagcga
    33001 gtcagaagtg atcaaacttt atactatttt ccgcaggagg attttccaaa gcaagatgtt
    33061 ctcaaatggc gcggccaatt aactcctttt caagagaagg tgtcagaggg attgcttcaa
    33121 gtagtagaca agcaaaagcc aaccttagtt catgcggtaa caggagctgg aaagacagaa
    33181 atgatttatc aagtagtggc taaagtgatc aatgcgggtg gtgcagtgtg tttggctagt
    33241 cctcgcatag atgtttgttt ggagctgtac aagcgcctgc aacaggattt ttcttgcggg
    33301 atagctttgc tacatggaga atcggaacct tattttcgaa caccactagt tgttgcaaca
    33361 acccatcagt tattgaagtt ttatcaagct tttgatttgc tgatagtgga tgaagtagat
    33421 gcttttcctt atgttgataa tcccatgctt taccacgctg tcaagaatag tgtaaaggag
    33481 aatggattga gaatcttttt aacagcgact tcgaccaatg agttagataa aaaggtccgt
    33541 ttaggagaac taaaaagact gaatttaccg agacggtttc atggaaatcc gttgattatt
    33601 ccaaaaccaa tttggttatc ggattttaat cgctacttag acaagaatcg tttgtcacca
    33661 aagttaaagt cctatattga gaagcagaga aagacagctt atccgttact catttttgct
    33721 tcagaaatta agaaagggga gcagttagca gaaatcttac aggagcaatt tccaaatgag
    33781 aaaattggct ttgtatcttc tgtaacagag gatcgattag agcaagtaca agcttttcga
    33841 gatggagaac tgacaatact tatcagtacg acaatcttgg agcgcggagt taccttccct
    33901 tgtgtggatg ttttcgtagt agaggccaat catcgtttgt ttaccaagtc tagtttgatt
    33961 cagattggtg gacgagttgg acgaagcatg gatagaccga caggagattt gcttttcttc
    34021 catgatgggt taaatgcttc aatcaagaag gcgattaagg aaattcagat gatgaataag
    34081 gaggctggtc tatgaagtgc ttgttatgtg ggcagactat gaagactgtt ttaactttta
    34141 gtagtctctt acttctgagg aatgatgact cttgtctttg ttcagactgt gattctactt
    34201 ttgaaagaat tggggaagag aactgtccaa attgtatgaa aacagagttg tcaacaaagt
    34261 gtcaagattg tcaattttgg tgtaaagagg gagttgaagt cagtcataga gcgattttta
    34321 cttacaatca agctatgaag gattttttca gtcggtataa gtttgatgga gacttcctgt
    34381 taagaaaagt tttcgcttca tttttaagtg aggagttgaa aaagtacaaa gagtatcaat
    34441 ttgttgtaat tcccctaagt cctgatagat atgctaacag aggatttaat caggttgagg
    34501 gcttggtaga ggcagcaggc tttgagtatc tagatttatt agagaaaaga gaagagagag
    34561 ccagttcttc taaaaatcgt tcagagcgct tggggacaga acttcctttc tttattaaaa
    34621 gtggagtcac tattcctaaa aaaatcctac ttatagatga tatctatact acaggagcaa
    34681 ctataaatcg tgttaagaaa ctgttggaag aagccggtgc taaggatgta aaaacatttt
    34741 cccttgtaag atgaggaaaa aatttgcaaa aatagaatga aagcgttata ataaaatcat
    34801 aaaaacaaaa atgtttagaa agaaggtact catatgatta aatatagtat ccgtggtgaa
    34861 aacctagaag taacagaagc aattcgtgat tatgtagttt ctaaactcga aaagatcgaa
    34921 aagtacttcc aaccagaaca agagttggat gcccgaatta acttaaaagt ttatcgtgaa
    34981 aaaacggcta aagtggaagt aacgattccg cttggatcta ttactctccg cgcagaagat
    35041 gtatctcaag atatgtatgg ttcaattgac cttgtaactg ataaaattga acgtcagatt
    35101 cgtaaaaata aaacaaaaat cgagcgtaaa aataaaaata aggtagcaac tggtcaatta
    35161 tttacagatg ctttggtgga agattcaaat attgtccagt ctaaagttgt tcgttcaaaa
    35221 caaattgatt taaaaccaat ggatttggaa gaagcaattc tacaaatgga tttattgggg
    35281 catgatttct ttatctatgt ggatgttgaa gatcagacaa ccaatgtgat ttatcgtcgt
    35341 gaggatggcg aaattggttt gttagaggtt aaagaatctt aatttcaata tgtgtaaagg
    35401 taggtttact gaattgtaaa ctaccttttt cttataattg atagaattac tgattacact
    35461 tttaaaaagt cgctttttga ggggtattat ggtataatgg gtgccaagag gttttgaatg
    35521 aaaaaatttt atgtaagtcc aatttttcct attctagtag gattgattgc gtttggagtc
    35581 ttatccactt tcattatttt tgttaataat aatctgttga cggttttaat tttgtttctt
    35641 tttgtaggag gctatgtttt tttatttaag aaactgagag tgcattatac aaggagtgat
    35701 gtagaacaga tacagtatgt aaaccaccaa gcggaagaaa gtttgacagc tctattggaa
    35761 cagatgcctg taggtgttat gaaattgaat ttatcttctg gagaggttga gtggtttaat
    35821 ccctatgctg aattgatttt gaccaaggaa gatggtgatt ttgatttaga agctgttcaa
    35881 acgattatca aggcttcagt aggaaatccg tctacttatg ccaagcttgg tgagaagcgt
    35941 tatgctgttc atatggatgc ttcttccggt gttttgtatt ttgtagatgt atccagggaa
    36001 caagccataa cagatgaatt ggtaacaagt agaccagtga ttgggattgt ctctgtggat
    36061 aattatgatg atttggagga tgaaacttct gagtcagata ttagtcaaat caatagtttt
    36121 gtagctaatt ttatatcaga gttttcagaa aaacacatga tgttttctcg tcgggtaagt
    36181 atggatcgat tttatctatt tactgactac acggtgcttg agggcttgat gaatgataaa
    36241 ttttctgtta ttgatgcttt cagagaagag tcgaaacaga gacagttgcc cttgacctta
    36301 agtatgggat tttcttatgg cgatggaaat catgatgaga tagggaaagt tgctttgctc
    36361 aatttgaact tggctgaagt acgtggtggc gaccaggtgg ttgttaagga aaacgacgaa
    36421 acgaaaaatc cagtttattt tggtggtggg tctgctgctt caatcaagcg tacacggact
    36481 cgtacgcgcg ctatgatgac agctatttca gataagattc ggagtgtaga tcaggttttt
    36541 gtagtcggtc acaaaaattt agacatggat gctttgggct ctgctgtagg tatgcagttg
    36601 ttcgccagca atgtgattga aaatagctat gctctttatg atgaagaaca aatgtctcca
    36661 gatattgaac gagctgtttc attcatagaa aaagaaggag ttacgaagtt gttgtctgtt
    36721 aaggatgcaa tggggatggt gaccaatcgt tctttgttga ttcttgtaga ccattcaaag
    36781 acagccttaa cattatcaaa agaattttat gatttattta cccaaaccat tgttattgac
    36841 caccatagaa gggatcagga ttttccagat aatgcggtta ttacttatat cgaaagtggt
    36901 gcaagtagtg ccagtgagtt ggtaacggaa ttgattcagt tccagaattc taagaaaaat
    36961 cgtttgagtc gtatgcaagc aagtgttttg atggctggta tgatgttgga tactaaaaat
    37021 ttcacctcgc gagtaactag tcggacattt gatgttgcta gctatctcag aacgcgcgga
    37081 agtgatagta ttgctatcca ggaaatcgct gcgacagatt ttgaagaata tcgtgaggtc
    37141 aatgaactta ttttacaggg gcgtaaatta ggttcagatg tactaatagc agaggctaag
    37201 gacacgaaat gctatgatac agttgttatt agtaaggcag cagatgccat gttagccatg
    37261 tcaggtattg aagcgagttt tgttcttgcg aagaatacac aaggatttat ctctatctca
    37321 gctcgaagtc gtagtaaact gaatgtacaa cggattatgg aagagttagg cggtggaggc
    37381 cactttaatt tggcagcagc tcaaattaaa gatgtaacct tgtcagaagc aggtgaaaaa
    37441 ctgacagaaa ttgtattaaa tgaaataaag gaaaaggaga aagaagaatg aaagtaatct
    37501 ttttagcaga tgttaaagga aaaggtaaaa aaggcgaaat taaggaagta ccaacagggt
    37561 atgcgcaaaa ctttcttatc aaaaagaatc tagccaaaga agcgactgct caagctgtag
    37621 gtgaacttcg tggtaaacaa aaatcggaag aaaaagctca cgctgagatg attgcagaag
    37681 ggaaagcaat taaagcacaa cttgaagcag aagaaactgt tgtagaattt gttgaaaaag
    37741 ttggtccaga tggtcgtacc tttggttcta ttaccaataa gaagattgca gaagaattac
    37801 aaaagcaatt tggaattaag attgataaac gtcatattca agtacaagct ccgattcgag
    37861 cggttggttt gattgatgtg ccagtgaaaa tctatcaaga tatcacaagt gtaatcaatc
    37921 ttcgtgtgaa agaaggataa gtttacacct tcttgacaag attgtaaaag gaagggaagt
    37981 ctgatggcag aagtagaaga gttacgagta caacctcaag atatcttagc tgagcaatcc
    38041 gttttagggg ctatctttat tgatgagagt aaacttgttt ttgtgcgaga atacattgag
    38101 tctcgggact tttttaagta tgcccatcgt ttgattttcc aagccatggt cgatttatcc
    38161 gatcgtggtg atgccataga tgcaacaacg gttcgtacta tccttgataa tcaaggtgat
    38221 ttacagaata ttggtggctt gtcttacttg gttgagattg tcaattctgt gccaacttct
    38281 gctaatgcgg agtattatgc taagattgtt gcagaaaaag caatgctacg tcgtttaatt
    38341 gccaagttga cagagtctgt caaccaagct tacgaagcgt cacaaccagc tgatgaaatt
    38401 attgctcagg cagaaaaagg gctgattgat gtcagtgaaa atgcaaatcg aagcgggttt
    38461 aagaacattc gagatgtgtt gaatctcaac tttggaaatc tggaagctcg ctcgcaacaa
    38521 acgaccgata ttacaggtat tgcgacaggt tatcgtgatt tggatcatat gacaacagga
    38581 cttcatgagg aggagttgat tatcttagca gctcgtccag cagttggtaa gacagcattt
    38641 gccttgaata tcgctcagaa tattgggact aagttggaca aaacggttgc tattttttca
    38701 ctcgaaatgg gtgcggaaag cttggtagac cgtatgttag ctgcagaagg cttggtggag
    38761 tcacattcta tccgtacagg gcaattgaca gatgaggagt ggcaaaaata tactattgct
    38821 cagggtaatc tagctaacgc cagtatctat atcgatgata cgccaggtat tcggattaca
    38881 gagattcgtt ctcgttctcg taaattggct caagaaactg gaaatcttgg tttgattttg
    38941 atagactatt tgcaacttat cacgggaact ggtcgagaaa atcgtcaaca agaagtttct
    39001 gaaatttctc gtcagttgaa aatactagcc aaggaattga aggttccagt aatcgctctg
    39061 agtcagcttt ctcgtggtgt agaacaacgt caggacaaga gaccggtctt gtctgatatt
    39121 cgtgaatctg ggtctattga gcaggacgct gatatcgtag cttttctcta tcgcgatgac
    39181 tactatgaac gtggtggtga agaagaggag ggtatcccaa ataataaggt ggaagttatt
    39241 atcgagaaaa accgtagtgg agctcgtgga acagtggaat tgattttcca aaaagaatac
    39301 aataaatttt caagtatctc aaagagggag gcataagatg tcagatgcat ttacagatgt
    39361 agccaagatg aaaaaaatca aagaagaaat caaggcacat gagggacaag tcgtagaaat
    39421 gactttggag aatggtcgta agcgccaaaa aaatagattg ggtaagctaa ttgaagttta
    39481 tccatcccta tttattgtgg agtttgggga tgtggaagga gataaacaag ttaatgttta
    39541 cgttgaatcc tttacttact cagatattct tacagaaaag aatttgattc attatcttga
    39601 ctaaagtgag aaattttctc actttttctt ttttctccga ataatttagg tgaaggcaat
    39661 catcgcttta tattattttt taaggaggaa gaatgaaaat tttaccgttt atagcaagag
    39721 gaacaagtta ttacttgaag atgtcagtta aaaagcttgt tcctttttta gtagtaggat
    39781 tgatgctagc agctggtgat agtgtctatg cctattccgg aggaaatgga tcgattgcgc
    39841 gtggggatga ttatcctgct tattataaaa atgggagcca ggagattgat cagtggcgca
    39901 tgtattctcg tcagtgtact tcttttgtag cctttcgttt gagtaatgtc aatggttttg
    39961 aaattccggc agcttatgga aatgcgaatg aatggggaca tcgtgctcgt cgggaaggtt
    40021 atcgtgtaga taatacaccg acgattggtt ccattacttg gtctactgca ggaacttatg
    40081 gtcatgttgc ctgggtgtca aatgtaatgg gagatcagat tgagattgag gaatataact
    40141 atggttatac agaatcctat aataaacgaa ttataaaagc aaacacgatg acaggattta
    40201 ttcattttaa agatttggat ggtggcagtg ttgggaatag tcaatcctca acttcaacag
    40261 gcgaaactca ttattttaag accaagtctg ctattaaaac tgaaccccta gttagtgcaa
    40321 ctgtgattga ttactattat cctggagaga aggttcatta tgatcaaatt ctcgaaaaag
    40381 acggctacaa gtggttgagt tatactgcct ataatggaag ctatcgttat gttcaattgg
    40441 aggctgtgaa taaaaatcct ctaggtaatt ctgttctttc ttcaacaggt ggaactcatt
    40501 attttaagac caagtctgct atcaaaactg aacccctagt tagtgcaact gtgattgatt
    40561 actattatcc tggggagaag gttcattatg atcagatact tgaaaaagac ggctacaagt
    40621 ggttgagtta tacggcttat aacggaagtc gtcgctatat acagctagag ggagtgactt
    40681 cttcacaaaa ttatcagaat caatcaggaa atatctctag ctatggatcc aataatagtt
    40741 caactgtcgg ttggaagaaa ataaatggta gttggtatca tttcaaatca aatggttcta
    40801 aatcaacagg atggctgaaa gatggttcta gctggtatta tttgaaatta tctggtgaaa
    40861 tgcagacagg atggttaaag gagaatggtt cgtggtatta tctgggtagt tcaggggcaa
    40921 tgaaaacagg ctggtaccag gtctctggta agtggtatta ttcttactct tcaggcgcct
    40981 tagctgttaa tacgacggtg gatggctaca gagtaaacag tgatggagaa cgagtataga
    41041 aaattggagt aggagaaatt tcctgctctt ttctgtaagc agtttccttg acatttttct
    41101 tattttgcgc tatcatatat acatataata tatgggagtc tgtgtacagt ctgagaggaa
    41161 gtgttaaact tcgaccgcac ctgatctggg taatgccagc ggagggaacg atacttagtc
    41221 taattttgca ccttttccat gtatggtaaa ggtttttctt ttttttaaaa ggaaaacgag
    41281 aagaggaggt tcttatgaaa gcaagcattg ccttgcaagt tttaccccta gtacagggga
    41341 ttgatcggat agctgttatt gatcaggtca ttgcttatct gcaaactcaa gaagtgccga
    41401 tggtagtgac accatttgaa acggtcttgg aaggggagtt tgatgagctt atgcgcattc
    41461 taaaagaagc gctggaagtg gcagggcagg aggcagacaa tgtctttgcc aatgtcaaaa
    41521 taaatgtagg agagatttta agtattgatg agaaacttga gaagtatact gagacgacac
    41581 attagtctat tgggctttct cggagtcttg tcaatctggc agttagcagg ttttcttaaa
    41641 ctgctcccca agtttatcct gccgacacct cttgaaattc tccagccctt tgttcgtgac
    41701 agagaatttc tctggcacca tagctgggcg accttgagag tggctttact ggggctgatt
    41761 ttgggagttt tgattgcctg tcttatggct gtactcatgg atagtttgac ttggctcaat
    41821 gacctgattt accctatgat ggtggtcatt cagaccattc cgaccattgc catagctcct
    41881 atcctggtct tgtggctagg ttatgggatt ttgcccaaga ttgtcttgat tatcttaacg
    41941 acaacctttc ccatcatcgt cagcattttg gacggtttta ggcattgcga caaggatatg
    42001 ctgaccttgt ttagtctgat gcgggccaag ccttggcaaa tcctgtggca ttttaaaatt
    42061 ccagtcagcc tgccctactt ttatgctggt ctgagggtca gtgtctccta cgcttttatc
    42121 acaacagtgg tatctgagtg gttgggaggc tttgaggggc ttggtgttta tatgattcag
    42181 tccaagaaat tgtttcagta tgataccatg ttcgctatta ttattctggt atcgattatc
    42241 agccttttgg gtatgaagtt ggtcgatatt agtgaaaaat atgttattaa atggaaacgt
    42301 tcgtagaatt agaatgtttc tgaaaaagaa aagaggaaat caaaatgaag aaaacatgga
    42361 aagtgttttt aacgcttgta acagctcttg tagctgttgt gcttgtggcc tgtggtcaag
    42421 gaactgcttc taaagacaac aaagaggcag aacttaagaa ggttgacttt atcctagact
    42481 ggacaccaaa taccaaccac acagggcttt atgttgccaa ggaaaaaggt tatttcaaag
    42541 aagctggagt ggatgttgat ttgaaattgc caccagaaga aagttcttct gacttggtta
    42601 tcaacggaaa ggcaccattt gcagtgtatt tccaagacta catggctaag aaattggaaa
    42661 aaggagcagg aatcactgcc gttgcagcta ttgttgaaca caatacatca ggaatcatct
    42721 ctcgtaaatc tgataatgta agcagtccaa aagacttggt tggtaagaaa tatgggacat
    42781 ggaatgaccc aactgaactt gctatgttga aaaccttggt agaatctcaa ggtggagact
    42841 ttgagaaggt tgaaaaagta ccaaataacg actcaaactc aatcacaccg attgccaatg
    42901 gcgtctttga tactgcttgg atttactacg gttgggatgg tatccttgct aaatctcaag
    42961 gtgtagatgc taacttcatg tacttgaaag actatgtcaa ggagtttgac tactattcac
    43021 cagttatcat cgcaaataac gactatctga aagataacaa agaagaagct cgcaaagtca
    43081 tccaagccat caaaaaaggc taccaatatg ccatggaaca tccagaagaa gctgcagata
    43141 ttctcatcaa gaatgcacct gaactcaagg aaaaacgtga ctttgtcatc gaatctcaaa
    43201 aatacttgtc aaaagaatac gcaagcgaca aggaaaaatg gggtcaattt gacgcagctc
    43261 gctggaatgc tttctacaaa tgggataaag aaaatggtat ccttaaagaa gacttgacag
    43321 acaaaggctt caccaacgaa tttgtgaaat aatgacagaa attagactag agcacgtcag
    43381 ttatgcctat ggtcaggaga ggattttaga ggatatcaac ctacaggtga cttcaggcga
    43441 agtggtttcc atcctaggcc caagtggtgt tggaaagacc accctcttta atctaatcgc
    43501 tgggatttta gaagttcagt cagggagaat tgtccttgat ggtgaagaaa atcccaaggg
    43561 gcgcgtgagt tatatgttgc aaaaggatct gctcttggag cacaagacgg tgcttggaaa
    43621 tatcattctg cccctcttga ttcaaaaggt ggataaggca gaagctattt cccgagcgga
    43681 taaaattctt gcgaccttcc agctgacagc tgtaagagac aagtatcctc atgaacttag
    43741 cggtgggatg cgccagcgtg tagccttact ccggacctac ctttttgggc acaagctctt
    43801 tctcttagat gaggccttta gcgccttgga tgagatgaca aagatggaac tccacgcttg
    43861 gtatcttgag attcacaaac agttgcagct aacaaccctg atcatcacgc atagtattga
    43921 ggaggccctc aatctcagcg accgcatcta tatcttgaaa aatcgccctg ggcagattgt
    43981 ttcagaaatt aaactagatt ggtctgaaga tgaggacaag gaagtccaaa agattgccta
    44041 caaacgtcaa atcttggcag aattaggctt agataagtag aaaaataggg agttggtgaa
    44101 gattatcctt taccagcgcc ctttttcttt taaaaatgag aaaatttcgg tataatagtc
    44161 aaacaaggtc aaggtttaaa gagagaggtg ggtttgttat gagatttaaa aatacatcgg
    44221 atcatattga ggcctacatc aaggcgattt tagatcaatc tggtatcgtg gagttgcaac
    44281 ggagtcagtt ggcagatacc tttcaggttg ttcctagtca gattaactac gtgatcaaga
    44341 cacgctttac ggaaagtaga ggctacttgg ttgaaagtaa gcgtggtggt ggaggctaca
    44401 ttcgtatagg acgaattgag ttttctagtc atcatgaaat gctccgggag ctgctttact
    44461 cgattggtga gcgagtcagt caagaaattt atgaggatat tctccagctt ttggttgagc
    44521 aggaattgat gaccaagcag gagatgaatt tgctagaatc agtagctttg gatcgcgttt
    44581 taggagaaga agctccagtt gttcgagcaa acatgctacg tcagatcata caagaggtag
    44641 atagaaaagg gaagtaagat gaactattca aaagcattga atgaatgtat cgaaagtgcc
    44701 tacatggttg ctggacattt tggagctcgt tatctagagt cttggcactt gttgattgcc
    44761 atgtctaatc acagttatag tgtagcaggg gcaactttaa atgattatcc gtatgagatg
    44821 gaccgtttag aagaagtggc tttggaactg actgaaacgg actatagcca ggatgaaacc
    44881 tttacggaat tgccgttctc ccgtcgtttg caggttcttt ttgacgaagc agagtatgta
    44941 gcgtcagtgg tctatgctaa ggtgctaggg acagagcacg tcctctatgc gattttgcat
    45001 gatggcaatg ccttggcgac tcgtatcttg gagagggctg gtttttctta tgaagacaag
    45061 aaagatcagg tcaagattgc tgctcttcgt cgaaatttag aagaacgggc aggctggact
    45121 cgtgaagatc tcaaggcttt acgccaacgc catcgtacag tagctgacaa gcaaaattct
    45181 atggccaata tgatgggcat gccgcagact cctagtggtg gtctcgagga ctatacgcat
    45241 gatttgacag agcaagcgcg ttctggcaag ttagaaccag tcatcggtcg ggacaaggaa
    45301 atctcacgta tgattcaaat cttgagccgg aagactaaga acaaccctgt cttggttggg
    45361 gatgctggtg tcgggaaaac agctctggcg cttggtcttg cccagcgtat tgctagtggt
    45421 gacgtgcctg cggaaatggc taagatgcgc gtgttagaac ttgatttgat gaatgtcgtt
    45481 gcagggacac gcttccgtgg tgactttgaa gaacgcatga ataatatcat caaggatatt
    45541 gaagaagatg gccaagtcat cctctttatc gatgaactcc acaccatcat gggttctggt
    45601 agcgggattg attcgactct ggatgcggcc aatatcttga aaccagcctt ggcgcgtgga
    45661 actttgagaa cggttggtgc cactactcag gaagaatatc aaaaacatat cgaaaaagat
    45721 gcggcacttt ctcgtcgttt cgctaaagtg acgattgaag aaccaagtgt ggcagatagt
    45781 atgactattt tacaaggttt gaaggcgact tatgagaaac atcaccgtgt acaaatcaca
    45841 gatgaagcgg ttgaaacagc ggttaagatg gctcatcgtt atttaaccag tcgtcacttg
    45901 ccagactctg ctatcgacct cttggacgaa gcagcagcaa cagtgcaaaa taaggcaaag
    45961 catgtaaaag cagacgattc agatttgagt ccagctgaca aggccctgat ggatggcaag
    46021 tggaaacagg cagcccagct aatcgcaaaa gaagaggaag tacctgtcta caaagacttg
    46081 gtgacagagt ctgatatttt gaccaccttg agtcgcttgt caggaatccc agttcaaaag
    46141 ctgactcaga ctgacgctaa gaaatatcta aatcttgaag cagaactcca taaacgggtt
    46201 atcggtcaag atcaagctgt ttcaagtatt agtcgtgcga ttcgccgtaa tcagtcagga
    46261 attcgcagtc acaagcgtcc gatcggttcc tttatgttcc taggacccac aggtgtcggt
    46321 aagaccgagt tggccaaggc tttggcagaa gttctctttg atgacgaatc agcccttatc
    46381 cgctttgata tgagtgagta tatggagaaa ttcgcagcca gccgtctaaa tggagctcct
    46441 ccaggctatg tgggttacga agaaggtggg gagttgacag agaaggttcg caataaaccc
    46501 tattccgttc tcctctttga tgaggtagag aaggctcacc cagatatctt taatgttctc
    46561 ttgcaggttc tggatgacgg tgtcttgaca gatagcaagg gacgcaaggt cgatttttca
    46621 aataccatta tcattatgac atcgaaccta ggtgcgactg cccttcgtga tgataagact
    46681 gttggttttg gggctaagga tattcgtttt gaccaggaaa atatggaaaa acgcatgttt
    46741 gaagaactga aaaaagctta tagaccggaa ttcatcaacc gtattgatga gaaggtggtc
    46801 ttccatagcc tatctagtga ccatatgcag gaagtggtga agattatggt caagccttta
    46861 gtggcaagtt tggctgaaaa aggcattgac ttgaaattac aagcttcagc tctgaaattg
    46921 ttagcaaatc aaggatatga cccagagatg ggagctcgcc cacttcgcag aaccctgcaa
    46981 acagaagtgg aggacaagtt ggcagaactt cttcttaagg gagatttagt ggcaggcagc
    47041 acacttaaga ttggtgtcaa agcaggccag ttaaaatttg atattgcata aaagaataaa
    47101 agtatcagca tctgaccata agtcacagtg gagtgaaact caatgaaaat caaaga
//
LOCUS       DBERDK010000008        44866 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.008, whole genome shotgun sequence.
ACCESSION   DBERDK010000008 DBERDK010000000
VERSION     DBERDK010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 44866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44866
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.008"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..89)
                     /locus_tag="ACL73L_000409"
     CDS             complement(<1..89)
                     /locus_tag="ACL73L_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HGS0771452.1"
                     /translation="MNDEASKQLTDARFKRLVGVQRTTFEEILA"
     gene            66..338
                     /locus_tag="ACL73L_000410"
     CDS             66..338
                     /locus_tag="ACL73L_000410"
                     /inference="COORDINATES: protein motif:HMM:NF036719.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HGS0771453.1"
                     /translation="MFTCFIIHRTTIPYFVSQEVYWKVRNIEAEAIRRNCERGAIFSG
                     KIKYHEDSQFKGDHYVECYAVLDNTVIARDRITVPIDPLCGKDFIE"
     gene            532..1866
                     /gene="trmFO"
                     /locus_tag="ACL73L_000411"
     CDS             532..1866
                     /gene="trmFO"
                     /locus_tag="ACL73L_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HGS0771454.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVANHPLIEVVRDEITELPTDVITVIATGPLTSDALAEKIHALNNGDGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEVIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
     gene            1950..2687
                     /gene="pyrH"
                     /locus_tag="ACL73L_000412"
     CDS             1950..2687
                     /gene="pyrH"
                     /locus_tag="ACL73L_000412"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HGS0771455.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHRDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQSGNIKRVVFGENIGTTVSNNIEEKE"
     gene            2696..3253
                     /gene="frr"
                     /locus_tag="ACL73L_000413"
     CDS             2696..3253
                     /gene="frr"
                     /locus_tag="ACL73L_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HGS0771456.1"
                     /translation="MANVIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRVHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASDLGITPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKVAVRNIRRDAMDEAKKQEKAKEITEDELKTLEKDI
                     QKVTDDAVKHIDDMTANKEKELLEV"
     gene            3313..4167
                     /gene="cvfB"
                     /locus_tag="ACL73L_000414"
     CDS             3313..4167
                     /gene="cvfB"
                     /locus_tag="ACL73L_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HGS0771457.1"
                     /translation="MNTNLASFIVGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKFRLTTLEVTATQDQFGWGRVTEVRKDLGVFVDTGLPDKEIVVSLDI
                     LPELKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            4176..4391
                     /locus_tag="ACL73L_000415"
     CDS             4176..4391
                     /locus_tag="ACL73L_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HGS0771458.1"
                     /translation="MRKSFYTWLMTERNPKSNSPKAILADLAFEEAAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDSIWQEYLEH"
     gene            4477..5445
                     /locus_tag="ACL73L_000416"
     CDS             4477..5445
                     /locus_tag="ACL73L_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HGS0771459.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEESACEEARQVIQALMVLVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQEKLKNIHQIDFVHFSAKDVVRHPVVAQIIRAYEYSTEVAHD"
     gene            5638..6138
                     /locus_tag="ACL73L_000417"
     CDS             5638..6138
                     /locus_tag="ACL73L_000417"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS0771460.1"
                     /translation="MIDQLSKYYSCRILTEKDIPSILSLYESNPLYFQHCPPEPNFAT
                     VKEDMLCLPEGKAKADKFFVGFWNGSDLVAVMDFVYAYPDEETVFIGLFMVDQAYQRK
                     GIGSHIVTEALAYFAKNFRKARLAYVKGNPQSQHFWEKQGFKSIGCEVKQELYTVVIA
                     EQSLED"
     gene            6141..6467
                     /locus_tag="ACL73L_000418"
                     /pseudo
     CDS             6141..6467
                     /locus_tag="ACL73L_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035616.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
     gene            complement(6768..7879)
                     /gene="ald"
                     /locus_tag="ACL73L_000419"
                     /pseudo
     CDS             complement(6768..7879)
                     /gene="ald"
                     /locus_tag="ACL73L_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003018852.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0042853 - L-alanine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
     gene            8056..8479
                     /locus_tag="ACL73L_000420"
                     /pseudo
     CDS             8056..8479
                     /locus_tag="ACL73L_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8547..9197
                     /locus_tag="ACL73L_000421"
     CDS             8547..9197
                     /locus_tag="ACL73L_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HGS0771461.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQ
                     AEVAAVSKDSSTGKEVNKEEKEEPVEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVQK
                     AGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGSGTASSTSKEKKVNLNKA
                     SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGAKTIEKLKDYVTVD"
     gene            9181..11421
                     /locus_tag="ACL73L_000422"
     CDS             9181..11421
                     /locus_tag="ACL73L_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HGS0771462.1"
                     /translation="MLQWIKNFSIPLIYLSFLLLWLYYAIFSTSYLALLGFVFLLVCL
                     FFQFPWKSASKVLVICGIFGFWFVFQNWQQSQASQNLADSVEGVRILPDTIKVNGDSL
                     SFRGKSNGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFNYQAY
                     LKTQGIYQTLNIKTIQSLQKIGSWDIGENLSSLRRKAVVWIKTHFPGPMRNYMTGLLL
                     GHLDTDFEEMNELYSSLGIIHLFALSGMQVGFFMNGFKKLLLRLGLTQEKLKWLTYPF
                     SLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIVMPNFFLIAGGVLSC
                     AYAFILTMTSKEGEGLKAVASESLVISLGILPILSFYFAEFQPWSILLTFVFSFLFDL
                     TFLPLLSILFVLSFLYPVIQLNFIFEWLEGIIRLVSQVARRPLVFGQPNAWLLILLLI
                     SLALVYDLRKNIKGLTVLSLLITGLFFLTKYPLENEITMLDVGQGESIFLRDVTGKTI
                     LIDVGGKAESYKKIEKWQEKMTTSNAQRTLIPYLKSRGVAKIDQLILTNTDKENVGDL
                     SEVTKAFHVGEILVSKDSLKQKEFVAELQATQTKVRSMTVGENLPIFGSQLEVLSPRK
                     MGDGGHDDTLVLYGKFLDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQHGNKKSS
                     SPAFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGINSKVYRTDQQGAIRFKGLDSW
                     KIESVR"
     gene            11600..11788
                     /locus_tag="ACL73L_000423"
     CDS             11600..11788
                     /locus_tag="ACL73L_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771463.1"
                     /translation="MTVTKSYKYDWNTVWEYSTNYHDHQYAWIPSWSRYDSYSEYKVG
                     GGWNYGRYEVINYYSGGY"
     gene            11820..12407
                     /locus_tag="ACL73L_000424"
     CDS             11820..12407
                     /locus_tag="ACL73L_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGS0771464.1"
                     /translation="MLNITHVTLKTRQVILQDVDFTFKKGRVYGLLAINGSGKTTLFR
                     AISNLIPISSGNISAPPSLFYYESIEWLDGNLSGMDYLRLIKNIWKSGLNLRDEIAYW
                     EMSDYISLPIRKYSLGMKQRLVIAMYFLSQAKCWLMDEITNGLDEYYRQKFFDRLAQI
                     DRQEQLVLLSSHYKEELVDICDRVVTIHQGQIEEV"
     gene            12411..13591
                     /locus_tag="ACL73L_000425"
                     /pseudo
     CDS             12411..13591
                     /locus_tag="ACL73L_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     misc_feature    13751..13882
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            13898..14428
                     /gene="infC"
                     /locus_tag="ACL73L_000426"
     CDS             13898..14428
                     /gene="infC"
                     /locus_tag="ACL73L_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HGS0771465.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPATDKK"
     gene            14461..14661
                     /gene="rpmI"
                     /locus_tag="ACL73L_000427"
     CDS             14461..14661
                     /gene="rpmI"
                     /locus_tag="ACL73L_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HGS0771466.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKASMVHSGDYKRIKAMLTRLK"
     gene            14713..15072
                     /gene="rplT"
                     /locus_tag="ACL73L_000428"
     CDS             14713..15072
                     /gene="rplT"
                     /locus_tag="ACL73L_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HGS0771467.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            15130..15510
                     /locus_tag="ACL73L_000429"
     CDS             15130..15510
                     /locus_tag="ACL73L_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGS0771468.1"
                     /translation="MASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGL
                     PGTTPNYYFVKDPDGYKVEVIREK"
     gene            15760..16560
                     /locus_tag="ACL73L_000430"
     CDS             15760..16560
                     /locus_tag="ACL73L_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HGS0771469.1"
                     /translation="MNLTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMVEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIEGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKVVTVLGFANKDAVILK
                     TELAQYGQVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDDHP
                     RAYLSLESRMACGMGACYACVLKVPESETVSQRVCEDGPVFRTGTVVL"
     gene            16571..17509
                     /locus_tag="ACL73L_000431"
     CDS             16571..17509
                     /locus_tag="ACL73L_000431"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HGS0771470.1"
                     /translation="MTTNRLQVSLPGLDLKNPIIPASGCFVFGQEYAKYYDLDLLGSI
                     MIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEVVLAEKLPWLEREYPNLPIIA
                     NVAGFSKQEYAAVSHGISKATNVKAIELNISCPNVDHCNHGLLIGQDPDLAYDVVKAA
                     VEASEVPVYVKLTPSVTDIVTVAKAAEDAGASGLTMINTLVGMRFDLKTRKPILANGT
                     GGMSGPAVFPVALKLIRQVAQTTDLPIIGMGGVDSAEAALEMYLAGASAIGVGTANFT
                     NPYACPDIIENLPKVMDKYGISSLEELRQEVKESLR"
     gene            17551..19584
                     /locus_tag="ACL73L_000432"
     CDS             17551..19584
                     /locus_tag="ACL73L_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HGS0771471.1"
                     /translation="MNLGEFWYNKINKNRGRRLMKKVRFIFLALLFFLASPEGAMASD
                     GTWQGKQYLKEDGSQAANEWVFDTHYQSWFYIKADANYADNEWLKQGDDYFYLKSGGY
                     MAKSEWVEDKGVFYYLDQDGKMKRNAWVGTSYVGATGAKVIEDWVYDSQYDAWFYIKA
                     DGQHAEKEWLQIKGKDYYFKSGGYLLTSQWINQAYVNASGAKVQQGWLFDKQYQSWFY
                     IKENGNYADKEWIFENGHYYYLKSGGYMAANEWIWDKESWFYLKFDGKIAEKEWVYDS
                     HSQAWYYFKSGGYMTANEWIWDKESWFYLKSDGKIAEKEWVYDSHSQAWYYFKSGGYM
                     TANEWIWDKESWFYLKSDGKMAEKEWVYDSHSQAWYYFKSGGYMAKNETVDGYQLGSD
                     GKWLGGKATNENAAYYQVVPVTANVYDSDGEKLSYISQGSVVWLDKDRKSDDKRLAIT
                     ISGLSGYMKTEDLQALDASKDFIPYYESDGHRFYHYVAQNASIPVASHLSDMEVGKKY
                     YSADGLHFDGFKLENPFLFKDLTEATNYSAEELDKVFSLLNINNSLLENKGATFKEAE
                     EHYHINALYLLAHSALESNWGRSKIAKDKNNFFGITAYDTTPYLSAKTFDDVDKGILG
                     ATKWIKENYIDRGRTFLGNKASGMNVEYASDPYWGEKIASVMMKINEKLGGKD"
     gene            complement(19645..21300)
                     /gene="pavA"
                     /locus_tag="ACL73L_000433"
     CDS             complement(19645..21300)
                     /gene="pavA"
                     /locus_tag="ACL73L_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HGS0771472.1"
                     /translation="MSFDGFFLHHIVEELRSELVNGRIQKINQPFEQELVLQIRSNRQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEI
                     TVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTKSLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQETKPCLTETSFSPVPFANQVGEPFANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILD
                     NYYTNQPIMIALDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRRQREKIQKRKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS"
     gene            21405..21902
                     /gene="ybeY"
                     /locus_tag="ACL73L_000434"
     CDS             21405..21902
                     /gene="ybeY"
                     /locus_tag="ACL73L_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HGS0771473.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAKKLGKEDKEMAVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPELEIAFDEEDLLENPELAEMMSEFDAYIGEL
                     FISIDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            21883..22278
                     /locus_tag="ACL73L_000435"
     CDS             21883..22278
                     /locus_tag="ACL73L_000435"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HGS0771474.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFAITGIFTAIKEERNMRKHAVTAL
                     VVILAGFVFQVSRIEWLFLLLSIFLVVAFEIINSAIENVVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAALTGALIFLPRIWDLLF"
     gene            22295..23194
                     /gene="era"
                     /locus_tag="ACL73L_000436"
     CDS             22295..23194
                     /gene="era"
                     /locus_tag="ACL73L_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HGS0771475.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKAAKVPVILVVNKIDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSR
                     LVDILSENLDEGFQYFPSDQITDHPERFLVSEMVREKVLHLTREEIPHSVAVVVDSMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEREY"
     gene            23243..24067
                     /gene="mutM"
                     /locus_tag="ACL73L_000437"
     CDS             23243..24067
                     /gene="mutM"
                     /locus_tag="ACL73L_000437"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HGS0771476.1"
                     /translation="MPELPEVETVCRGLEKLIIGKKISSIEIRYPKMIKTDLEEFQRE
                     LPSQIIESMGRRGKYLLFYLTDKVLISHLRMEGKYFYYPDQGPERKHAHVFFHFEDGG
                     TLVYEDVRKFGTMELLVPDLLDAYFISKKLGPEPSEQDFDLQVFQAALAKSKKPIKSH
                     LLDQTLVAGLGNIYVDEVLWRAQVHPARPSQTLTAEEATAIHDQTIAVLGQAVEKGGS
                     TIRTYTNAFGEDGSMQDFHQVYDKTGQECVRCGTIIEKIQLGGRGTHFCPNCQRRD"
     gene            24067..24672
                     /gene="coaE"
                     /locus_tag="ACL73L_000438"
     CDS             24067..24672
                     /gene="coaE"
                     /locus_tag="ACL73L_000438"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HGS0771477.1"
                     /translation="MRKIIGITGGIASGKSTVTNFLRQQGFQVVDADAVVHQLQKSGG
                     RLFEALVQHFGQEIILENGELNRPLLASLIFSNPEEQKWSKQIQGEIIREELATLREQ
                     LAQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDAQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            24659..25858
                     /locus_tag="ACL73L_000439"
     CDS             24659..25858
                     /locus_tag="ACL73L_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HGS0771478.1"
                     /translation="MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSQQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
                     LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLF
                     LTSFVIQFSAQSIGPILALYVRDLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDK
                     VGNHRLLVVAQFYLVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPK
                     AGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRT
                     LLKVKEI"
     gene            25858..26007
                     /gene="rpmG"
                     /locus_tag="ACL73L_000440"
     CDS             25858..26007
                     /gene="rpmG"
                     /locus_tag="ACL73L_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HGS0771479.1"
                     /translation="MRVKINLKCSSCDSINYLTSKNSKTHPDKIEVLKYCPKERKVTL
                     HLESK"
     gene            26047..26280
                     /gene="secG"
                     /locus_tag="ACL73L_000441"
     CDS             26047..26280
                     /gene="secG"
                     /locus_tag="ACL73L_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HGS0771480.1"
                     /translation="MYNLLLTILLVLSVVIVIAIFMQPTKNQSSNVFDASSGDLFESS
                     KARGFEAVMQRLTGILVFFWLAIALALTVLSSR"
     gene            26382..28736
                     /gene="rnr"
                     /locus_tag="ACL73L_000442"
     CDS             26382..28736
                     /gene="rnr"
                     /locus_tag="ACL73L_000442"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HGS0771481.1"
                     /translation="MKDRIKEYLQDKGKVTVNDLAQALGKDSSKDFRELIKTLSLMER
                     KHQIRFEEDGSLTLEAKKKHEITLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAI
                     DGDTVEVVIKKVADRNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNQ
                     KISQPIYVKKPALKLEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVL
                     ESMDIVSEFPEAVVKEAESVPDAPSQKDMEGRLDLRDEITFTIDGADAKDLDDAVHIK
                     ALKNGNLELGVHIADVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSL
                     NPQVDRLTQSAIMEIDKHGRVVNYTIIQTVIKTSFRMTYSDVNDILAVDEEKRKEYHK
                     IVSSIELMAKLHETLENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERMIE
                     SFMLMANETVAEHFSKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLRIYGTASEISQE
                     ALQDIMRAVEGEPYADVLSMMLLRSMQQARYSEHNHGHYGLAADYYTHFTSPIRRYPD
                     LLVHRMIRDYGRSKEIAEHFEQVIPEIATQSSNRERRAIEAEREVEAMKKAEYMEEYV
                     GEEYDAVVSSIVKFGLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVG
                     QQIRIRVERADKMTGEIDFSFVPSEFDVIEKGLKQSSHSGRGRGSNRRSDKKEDKRKS
                     GRSNDKRKHSQKDKKKKGKKPFYKEVAKKGAKHGKGRGKGRRTK"
     gene            28699..29166
                     /gene="smpB"
                     /locus_tag="ACL73L_000443"
     CDS             28699..29166
                     /gene="smpB"
                     /locus_tag="ACL73L_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HGS0771482.1"
                     /translation="MAKGEGKVVAQNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NLKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKKQIQKLEQETKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            29182..30042
                     /gene="tehB"
                     /locus_tag="ACL73L_000444"
     CDS             29182..30042
                     /gene="tehB"
                     /locus_tag="ACL73L_000444"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HGS0771483.1"
                     /translation="MEKLVAYKRMPLWNKQTMPEAVQQKHNTKVGTWGKITVLKGALK
                     FIELTEEGEVLAEHLFEAGADNPMAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKY
                     NTNPVHSEVLEAMQTVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSI
                     VEQEDLDMPVGLYDINSASIEQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYN
                     LIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLR
                     FATLLAKKIK"
     gene            30115..31068
                     /gene="coiA"
                     /locus_tag="ACL73L_000445"
     CDS             30115..31068
                     /gene="coiA"
                     /locus_tag="ACL73L_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HGS0771484.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYTCPACGGQIHLRQGPSVRTH
                     FAHKSLKDCDFFFENESPEHIANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGKKLWLKERLTCLQQGFLYFSQNMGF
                     YVWELDKEKQVLRLKYLIYQDLRGKLHYQIKEFSYGQGSLLEILRLPYKRQKISHFTV
                     SEDKDICRYIRQQLYYQNLFWMKEQAEAYQKGENILTYGLKEWYPQIRPIVGKFFQIK
                     QDLTSYYQHFYTYYQKNPQNDWQKLYPPAFYQQYFLKNMVE"
     gene            31087..32889
                     /gene="pepF"
                     /locus_tag="ACL73L_000446"
     CDS             31087..32889
                     /gene="pepF"
                     /locus_tag="ACL73L_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HGS0771485.1"
                     /translation="MVLQRNEINEKDTWDLSTIYPTDQAWEEALKDLTEQLETVAQYE
                     GHLLDSADNLLEITEFSLEMERQMEKLYVYAHMKNDQDTREAKYQEYYAKAMTLYSQL
                     DQAFSFYEPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLQGKDHVLSQREEELLAGA
                     GEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGTYTRLMESKKREVRRGAYQAL
                     YATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAAVRK
                     HLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYSFTYQEALKKAEDALAVLGEDYL
                     SRVKRAFSERWIDVYENQGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETGHSM
                     HSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLDGFR
                     GTVFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYAYLNQEYYGLSKEDNPEIQYEWA
                     RIPHFYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLKAGKSDYPLNVMRKA
                     GVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA"
     gene            32891..33604
                     /locus_tag="ACL73L_000447"
     CDS             32891..33604
                     /locus_tag="ACL73L_000447"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HGS0771486.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGFLKELEDFA
                     RKENIPIIPHETVAYFRFLMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQKLFDATLDNPELTATLVPLG
                     DGILMLRKNVADVQLSESE"
     gene            33671..34612
                     /gene="prsA"
                     /locus_tag="ACL73L_000448"
     CDS             33671..34612
                     /gene="prsA"
                     /locus_tag="ACL73L_000448"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HGS0771487.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDVITEHQFY
                     EQVKNNPSAQQVLLNMTIQKVFEKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKTEKSSNIDDYKEKLKTVILTQKQNDSTFVQSIIG
                     KELQAANIKVKDQAFQNIFTQYIGGGDSSSSSSTSNE"
     gene            complement(34731..35072)
                     /locus_tag="ACL73L_000449"
                     /pseudo
     CDS             complement(34731..35072)
                     /locus_tag="ACL73L_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            35491..35586
                     /locus_tag="ACL73L_000450"
                     /pseudo
     CDS             35491..35586
                     /locus_tag="ACL73L_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(35839..36459)
                     /locus_tag="ACL73L_000451"
     CDS             complement(35839..36459)
                     /locus_tag="ACL73L_000451"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HGS0771488.1"
                     /translation="MKLYFVRHGRTLWNQEGRFQGASGDSPLLPGSIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIIQSQLHTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGAESLYSTTQRTIQFIKSLKDSPAERILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTLNTWNDTSYQS"
     gene            complement(36459..36941)
                     /locus_tag="ACL73L_000452"
     CDS             complement(36459..36941)
                     /locus_tag="ACL73L_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HGS0771489.1"
                     /translation="MAKKVKIKKTLVEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGDKTGPIIGIVPITQHLSEKKLAKISGNKKMSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNYPIFIDKIALDLDRMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            complement(36941..37783)
                     /locus_tag="ACL73L_000453"
     CDS             complement(36941..37783)
                     /locus_tag="ACL73L_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HGS0771490.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGVLAAILGIYERMIGFLAH
                     PFKDFKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLAWLWTTFIISGLGLYALNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTFDLLGTFFPIGIGAGATLIVFSKLMDYALNNYHSR
                     VYHFIIGIVLSSTLLILIPNAGNAESIQYTGLSLVGYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            complement(37878..38678)
                     /locus_tag="ACL73L_000454"
     CDS             complement(37878..38678)
                     /locus_tag="ACL73L_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HGS0771491.1"
                     /translation="MRKQIHPLILFSFFGIMMFILIINRPLNDSQSFKTKSNIAQIEA
                     QALKHLDKPIIDLSGWQRPEEINYDALSQNISGAIVRVHSGAQTTKENDASFINGIDK
                     AYKSHITELQKRNVPVAVYAYVAGKSVQEMEKAAEVFYNAASPYSPSYYWLDVEDKTM
                     SNMNEGVENFRAKLASLGAKNIGIYVGVYFMEEHSIDTGKFTSVWIPFYGSDSGFLES
                     SPKTDLDYDIHQYTSKGKIAGFDHDLDINVISPLKNKEETFRKLFLKP"
     gene            38822..40201
                     /gene="glmU"
                     /locus_tag="ACL73L_000455"
     CDS             38822..40201
                     /gene="glmU"
                     /locus_tag="ACL73L_000455"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002899862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HGS0771492.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLAGQTEFVTQSEQLGTGHAVMMTEPILEGLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNERLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRVALATAESVMRRRINHKHMVNGVSFVNPEATYIDIDVEIASEVQIEA
                     NVILKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVIVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGNNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPKNQ"
     gene            40211..40756
                     /locus_tag="ACL73L_000456"
     CDS             40211..40756
                     /locus_tag="ACL73L_000456"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HGS0771493.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTAQRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
                     SGHICDAKTIMAVQYWELQKK"
     gene            40768..41082
                     /gene="macP"
                     /locus_tag="ACL73L_000457"
     CDS             40768..41082
                     /gene="macP"
                     /locus_tag="ACL73L_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HGS0771494.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKDHGFSQETLKIQVEPSIHKSRRIENTKRNVFNSKLNKILFAVIFLLILLVLAMK
                     LL"
     gene            41099..41791
                     /locus_tag="ACL73L_000458"
     CDS             41099..41791
                     /locus_tag="ACL73L_000458"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HGS0771495.1"
                     /translation="MKIGIIAAMPEELAYLVQHLDNAQEQVVLGNTYHTGTIASHEVV
                     LVESGIGKVMSAMSVAILADHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYAYGQMAQQPLYFESDKTFVAQIQESLSQLDQNWHLGLIATGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHALNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLTFLKALD"
     gene            41868..42359
                     /gene="rocS"
                     /locus_tag="ACL73L_000459"
     CDS             41868..42359
                     /gene="rocS"
                     /locus_tag="ACL73L_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HGS0771496.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            42457..43047
                     /locus_tag="ACL73L_000460"
     CDS             42457..43047
                     /locus_tag="ACL73L_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HGS0771497.1"
                     /translation="MERLEDYIAFDLEFNQHEGQTHLIQVSAVRFRDGREIDAYDSYV
                     HTSVPLKSFINGLTGITAETLKDAPPVEKVLEEFQEFVGSLLMIGYNAAKSDLPILAE
                     HGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVASFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGGLDLSQLFD"
     gene            43056..44690
                     /locus_tag="ACL73L_000461"
                     /pseudo
     CDS             43056..44690
                     /locus_tag="ACL73L_000461"
                     /inference="COORDINATES: protein motif:HMM:NF021615.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
BASE COUNT        13581 a         8204 c         9991 g        13090 t
ORIGIN      
        1 gctaatatct cttcaaaagt cgtgcgctga acaccaacaa gacgcttaaa tcgtgcatca
       61 gttagttgtt tacttgcttc atcattcata gaactactat accatatttt gtttcgcagg
      121 aagtctattg gaaagtaaga aatattgaag ctgaggctat tagaagaaat tgtgagcgtg
      181 gtgctatttt ttcaggtaaa ataaaatatc acgaagattc acagtttaaa ggagatcact
      241 atgttgaatg ttatgctgtt ttagataata cggttatagc aagagataga ataacagtcc
      301 ctatcgatcc gttatgtgga aaagatttta tagagtagca tataattgat tcttaactgg
      361 aatactcact atctctttac atcaagaaaa tgactaaaca gggaagtttg ccttcttccc
      421 tttttttgtt atactagtag aagaaaaaat tagaaagatt tgtgggtgtc aaacagccca
      481 gtggggtgtt ttaatatgga cttaggtccc acccaaagag gtattagtgt cgtgtctcaa
      541 tcttatatca atgttatcgg tgctggtttg gcaggttctg aagcagctta ccaaatcgca
      601 gagcgtggta ttccagttaa actatatgaa atgcgtggtg tcaagtctac accccagcat
      661 aaaacagaca attttgctga gttggtttgt tccaattctt tgcgtgggga tgctttgaca
      721 aatgcagttg gtcttctcaa ggaagaaatg cgtcgcttgg gttctgttat cttggaatct
      781 gctgaggcta cacgtgttcc tgcaggtggt gcccttgcag tggaccgtga tggtttctct
      841 caaatggtga ccgaaaaagt tgccaaccac cccttgattg aagtggttcg tgatgaaatt
      901 acagaattgc cgacagatgt tattacggtt atcgctactg gtcctttgac aagtgatgcc
      961 ttggctgaaa agattcatgc ccttaataat ggcgatggct tctatttcta cgatgcagca
     1021 gcgcctatta tcgatgtcaa cactatcgat atgagtaagg tctatctcaa atctcgttat
     1081 gataagggag aagcagccta tctcaatgct ccaatgacca agcaagagtt tatggatttc
     1141 catgaagcct tggtcaatgc ggaagaagca ccgctcaatt cttttgaaaa agaaaagtac
     1201 tttgaaggat gtatgccaat cgaagttatg gccaaacgtg gcattaaaac tatgctttat
     1261 ggccctatga agccagtcgg tcttgagtat ccggacgact acacaggacc tcgtgatgga
     1321 gaatttaaaa caccttatgc ggttgtgcaa cttcgtcagg ataatgcagc tggtagccta
     1381 tacaatattg ttggtttcca gacccacctc aaatggggag aacaaaagcg tgtcttccaa
     1441 atgattccgg gtcttgaaaa tgcggagttt gtccgttatg gtgtcatgca tcgcaattct
     1501 tacatggatt caccaaatct tcttgagcag acttaccgtt ccaagaaaca accaaatctc
     1561 ttctttgctg gtcagatgac gggggtggaa ggctatgttg agtcggcggc ttcgggctta
     1621 gttgcgggaa ttaacgcagc tcgtctcttc aaggaagaaa gcgaggttat tttccccgag
     1681 acgacagcga ttggaagctt agctcattac attacccatg ccgacagcaa acatttccaa
     1741 ccaatgaatg tcaattttgg gatcatcaag gagttggaag gcgagcgtat ccgtgataag
     1801 aaggctcgtt atgaaaaaat tgcagagcgt gcccttgccg acttagagga atttttgact
     1861 gtctaatttt tttgaaagaa ttgttcatga tactataaaa atcttagaaa ttgtgataaa
     1921 ataggtagga tgaaagaagg agagtgaaaa tggcgaatcc caagtataaa cgtattttaa
     1981 tcaagttatc aggtgaagcc cttgccggtg aacgtggcgt agggattgat atccaaacag
     2041 ttcaaacaat cgcaaaagag attcaagaag ttcatagctt aggtatcgaa attgcccttg
     2101 ttattggtgg aggaaatctc tggcgtggag aacctgcagc agaagcaggt atggaccgtg
     2161 ttcaggcaga ttacactgga atgcttggga ctgttatgaa tgctcttgtg atggcagatt
     2221 cattgcaaca agttggggtt gatacgcgtg tacaaacagc tattgctatg caacaagtgg
     2281 cagagcctta tgtccgtgga cgtgcccttc gtcaccttga aaaaggccgt atcgttatct
     2341 ttggtgctgg aattggttca ccatacttct cgacagatac aacagcggcc cttcgtgcag
     2401 ctgaaatcga agcagatgcc atcctcatgg ctaaaaatgg cgtcgatggt gtgtacaatg
     2461 ccgatcctaa gaaggacaag acagccgtta agtttgaaga attgacccac cgtgatgtta
     2521 tcaacaaagg tcttcgtatc atggactcaa cagcctcaac cctctcaatg gacaacgaca
     2581 ttgacttggt tgtcttcaac atgaaccaat caggcaacat caaacgtgtc gtatttggtg
     2641 aaaatatcgg aacaacagtt tcaaataata tcgaagaaaa ggaataagaa agaatatggc
     2701 taacgtaatt attgaaaaag ctaaagagag aatgacccag tctcaccaat cacttgctcg
     2761 tgaatttggt ggtatccgtg ctggtcgtgc caatgcaagc ttgcttgacc gtgtacatgt
     2821 agaatactat ggagtcgaaa ctcctcttaa ccaaatcgct tcaattacga ttccagaagc
     2881 gcgtgttttg ttggtaacac catttgacaa gtcttcattg aaagacatcg aacgtgcctt
     2941 gaacgcttct gatcttggta tcacaccggc taatgacggt tctgtgattc gcttggttat
     3001 cccagctctt acagaagaaa ctcgtcgtga ccttgctaaa gaagtgaaga aggtcggcga
     3061 aaatgctaaa gtggctgtcc gcaatatccg tcgcgatgct atggacgaag ctaagaaaca
     3121 agaaaaagca aaagaaatca ctgaagacga attgaagact cttgaaaaag acattcaaaa
     3181 agtaacagac gatgctgtta aacacatcga cgacatgact gctaacaaag agaaagaact
     3241 tttggaagtc taaaaataaa cagaaaaact cagttggcat tgctggctga gttttattcg
     3301 aaagaaggaa atatgaatac aaatcttgca agttttatcg ttggactgat catcgatgaa
     3361 aacgaccgtt tttactttgt gcaaaaggat ggtcaaacct atgctcttgc taaggaagaa
     3421 ggccaacata cagtagggga tacggtcaaa ggttttgcat acacggatat gaagcaaaaa
     3481 ttccgcctga caaccttaga agtgactgcc actcaggacc aatttggttg gggacgtgtc
     3541 acagaggttc gtaaggactt gggtgtcttt gtggatacag gccttcctga caaggaaatc
     3601 gttgtgtcac tcgatattct ccctgagctc aaggaactct ggcctaagaa gggcgaccaa
     3661 ctctacatcc gtcttgaagt agataagaaa gaccgtatct ggggcctctt ggcttatcaa
     3721 gaagacttcc aacgtcttgc tcgtcctgct tacaacaaca tgcagaacca aaactggcca
     3781 gccattgttt accgtctcaa gctgtcagga acttttgttt acctaccaga aaataatatg
     3841 cttggtttta ttcatcctag cgagcgttac gcagagccac gtttggggca agtattagat
     3901 gcgcgcgtta ttggtttccg tgaagtggac cgcactctga acctctccct caaaccacgc
     3961 tcctttgaaa tgttggaaaa cgatgctcag atgattttga cttatttgga aagcaatggc
     4021 ggtttcatga ccttaaatga caagtcatct ccagacgaca tcaaggcaac ctttggcatt
     4081 tctaaaggtc agttcaagaa agctttaggt ggtcttatga aggctggtaa aatcaagcaa
     4141 gaccagtttg ggacagagtt gatttaggga ggcttatgag aaaatcattt tacacttggc
     4201 tcatgaccga gcgcaatcct aaaagtaaca gtcccaaagc aattttggca gacctcgctt
     4261 ttgaagaggc agcttttcca aaacatacag atgattttga tgaggtcagt cgctttttgg
     4321 aggagcatgc cagtttctct tttaacctag gagattttga cagcatttgg caggaatatc
     4381 tagaacacta gcatttattc attgggtttg ggctagtaat ttctccatcc ctctgctata
     4441 ataaaaagaa ataaaaggat tagagaggtt ctttatttga aggaacattc aatagacatt
     4501 caactgagtc atccagatga cctgtttcat ctttttggtt ccaatgaacg ccatcttcgt
     4561 ttgatggaag aagagcttga tgttgtgatt catgctcgta cggagattgt ccaggttttg
     4621 ggagaagagt ctgcctgtga ggaagcccgt caagttattc aggctttgat ggtcttggta
     4681 aatcgtggga tgaccgttgg tacgccagat gtagtcactg cgattagcat ggtcaaaaac
     4741 gatgaaattg ataagtttgt cgccctttac gaagaagaaa ttatcaagga taatactggg
     4801 aaaccgatcc gcgtcaaaac cttaggtcaa aaactttatg tggacagtgt caaacagcat
     4861 gatgtgacct ttggaattgg gcctgcaggg acggggaaga cctttcttgc agttactttg
     4921 gcagtgactg cccttaaacg tgggcaagtc aagcggatta tcttgacacg tccagcggtg
     4981 gaagcaggag agagtctagg ttttcttccg ggtgatctta aggagaaggt agatccttac
     5041 cttcgtcctg tttacgatgc cttgtatcag attctgggga aagaccaaac gactcgtctc
     5101 atggagcgtg aaattatcga aattgcgccc cttgcctata tgcgtggtcg gaccttggat
     5161 gatgcctttg tcattctcga tgaggcgcaa aacacgacca tcatgcagat gaagatgttc
     5221 ttgacgcgtt taggctttca ttctaagatg attgtcaatg gagatatcag tcagattgac
     5281 ctgccacgca atgtcaagtc aggtttgatt gatgcccaag agaaactcaa gaacatccat
     5341 cagattgact ttgttcattt ttcagctaag gatgtggttc gccatccagt tgtcgctcag
     5401 attatccgag cttatgaata ttctactgaa gttgcacacg actgattttg aggaagttcg
     5461 cctgcaaaag aatatacttt tgagagagga aaatccagtt gtataggcta aaggttttat
     5521 ccaaaggttt gagacaacga ttaggcacga tggaaagaac ttttatgtgg ctgatgacga
     5581 tcagtgcatc ttcctgtgtc ataatcacag ggcacaagaa agtaggaatt tgaaaagatg
     5641 attgaccaac tatctaagta ttacagttgt aggatactaa ctgaaaagga tattccaagt
     5701 attttatctt tatatgaaag taatcctctg tattttcagc attgtccacc agaaccaaat
     5761 tttgcaactg taaaagagga catgctttgt ctacctgaag gtaaagctaa ggctgataag
     5821 ttttttgttg gattttggaa tggatctgac cttgtggctg ttatggattt tgtctatgca
     5881 tatcctgatg aggagactgt ttttattggt ttgtttatgg ttgatcaagc ctatcagagg
     5941 aaagggattg gtagtcatat tgtgacagaa gcactagctt attttgctaa gaactttcga
     6001 aaggcacgtt tggcttatgt taagggaaat ccgcaatctc agcatttttg ggaaaagcag
     6061 ggctttaaat caattggatg cgaggttaag caagaactct atacggttgt tatcgctgaa
     6121 cagagcctag aagattagaa atggcatcaa gtaagaacta tttggaattt gttttggaac
     6181 aattatcagg attagatgat gtgacttacc gttccatgat gggggagtat attctttact
     6241 tccgcggcaa gattattggc ggcatttatg acgatcgctt tttagttaaa cccgtgcaag
     6301 cagtcttaga taagattgac caatcttctt ttgagtttcc atacaaaggt gccaaagaaa
     6361 tgatttgagt ggaagaactt gataataaga tgtttctata agacctgatt ttagctatgt
     6421 ataaccaact gccaacgccc aaacctaaaa agaaaaagca agtgtgaacg aagtaaaaaa
     6481 gaagtctgct aaggccctgt ctttgcacgg gtaaaatttt atatataaaa agaagctggg
     6541 actaaagagc tcagcttcct ttggtttata taattgtcat tacaagacga agtggttggg
     6601 cgaaactctg ttgactttat tcaatttaga gtttcttatg cacgattgag tctggaacga
     6661 aagtctccag ttgcaaagta tacagtacaa taaaccaacg atgtaatagc tgatgacaca
     6721 aagcacagtg ggtaggactt gcgaagtcac ccttttcttt tcaaaattta tactaaatca
     6781 ttgatatcag tgtagtcacg attaagtcct tgagcaactg gtaggctagt caagtaacct
     6841 tgataagtgg tcacaccttg acgcaagcct tcatcttcag agattgcttg tgcgaatcct
     6901 ttgccagcca aagcttcgat ataaggaaga gtgacattgg ttagggcgat ggttgaagtg
     6961 tgggcaaccg caccagggat attggcaacg gcatagtgga gaacaccgtg tttttcatag
     7021 acgggttcat cgtgcgttgt cacacggtca gctgtttcga taaggccacc ttggtcaaca
     7081 gcaacgtcaa cgatacagag cctggacgca tttgtttgac catctcatct gtcaccaatt
     7141 ccggtgcttt tgcaccaggg atgagaatgg ctccaatcac cacatcagca tctctcacac
     7201 ttgcttcaat gttgaatgaa ttagatataa gagtttgaat ttgacttcca aagacttctt
     7261 ctagaactga gagacgcttg gcactaatat ctaaaatagt cacttgagca ccaagaccaa
     7321 gggcgatgcg ggcagcatgt gtaccgacga caccatcacc gatgatagtt acttttcctt
     7381 ttggaacacc tggtacacca ccaggtagaa caccagagcc accagcttgc ttagtaagga
     7441 agtgagctcc gatttaaaca gccatacgac ctgcaacctc actcatagga acgaggagcg
     7501 gtagttgtcc ttgattgtca cgaacagttt cataggcaat tcctgttgtt tttgctgcta
     7561 acatagcatc tgctaattct ggagcagcgg ccatgtgcaa gtaggtgaag agaagaagat
     7621 cgtcgcgcaa gtaaccgtat tcagaactta aaggttcttt tactttcaca accaactctg
     7681 ctacccaagc ttcaccagca gtagcgacaa tctcagctcc ttgcttttga tagtcagcat
     7741 cagtaaagcc agaaccgaga ccagcatttg tttcgataag gacacgatga ccacgactaa
     7801 ctaagctatg aacacctgca ggtgtgaggg cgacacggtt ttcgttattt ttaatttctt
     7861 ttgggattcc gattaacatt gagataacct acctttcaat tgacggtctt gttttggttg
     7921 tcacattcca gttcataaat caaaaatgtg acggtttcat tgtatatgaa accgcttcaa
     7981 aaatcaagaa aaacttgtca tcccaatttt tttatgctag actagtgaaa atcaagctct
     8041 aatggaggga aaagtatgga atcaattttt gtaaaacttg cccagtatcc gtctatagaa
     8101 acggagcgtt tattgcttag acctgtaact ttggatgatg cggaagcaat gtttgactat
     8161 gcctcggaca agggtaatac acgttacact tttccaacca atcaaagctt ggaagaaacc
     8221 aagaataaca ttgctcagtt ctacttggct aatcccttgg gacgttgggg aatagaacta
     8281 aaaagcaatg gtcagtttat tggaaccatt gacttgcaca agtaaggcta atcccgcgtc
     8341 aggaaaggtc atggagaaat caggcatgcg tttttcccat gcagaaccat atgcttgtat
     8401 ggaccagcat gaaaaaggcc gaatcgtgac aagagttcat tatgtcttga ccaaggaaga
     8461 ctattttgca aataaataag cagttgaaaa gaaatttttc gactgttttt tcttcctctt
     8521 acgaataatc taagagagga gaaaatatgg aagcaattat cgagaaaatc aaagagtata
     8581 aaatcatcgt catctgtact ggtctgggct tgcttgtagg aggatttttc ctgctaaagc
     8641 cagctccaca aacacctgtc aaagagacga atttgcaggc tgaagttgca gctgtttcca
     8701 aggactcatc gaccggaaag gaagtgaaca aggaagagaa ggaagaacca gttgaacaag
     8761 atctaatcac agtagatgtc aaaggtgctg tcaaatcgcc agggatttat gacttgcctg
     8821 taggtagtcg agtcaatgat gctgttcaga aggctggtgg cttgacagag caagcagaca
     8881 gcaagtcgct caatctagct cagaaagtta gtgatgaggc tctggtttac gttcctacta
     8941 agggagaaga agcagttagt caacagactg gttcggggac agcttcttca acaagcaagg
     9001 aaaagaaggt caatctcaac aaggccagtc tggaagaact caagcaggtc aagggactgg
     9061 gaggaaaacg agctcaggac attattgacc atcgtgaggc aaatggcaag ttcaagtcag
     9121 tagacgagct caagaaggtc tctggcattg gtgccaagac tatagaaaag ttaaaagact
     9181 atgttacagt ggattaagaa tttctctatt cccctaattt acctgagttt tctgttactt
     9241 tggctttact acgccatttt ttcaacatcc tatcttgctt tgttgggatt tgtttttctg
     9301 ctagtctgtc tctttttcca atttccttgg aaatctgcta gcaaagttct agtgatttgt
     9361 ggaatctttg gattttggtt tgtttttcaa aattggcaac agagtcaagc gagtcaaaat
     9421 ctggcggatt ctgttgaagg ggtacggatt ttgcctgata ctattaaggt taatggtgat
     9481 agtctatcct ttcgtggcaa gtctaacggt cgtgctttcc aagtctatta taaactccag
     9541 tccgaggagg agaaagaagc ctttcaagct ttaaccgacc tgcatgagat aggactagaa
     9601 gggaagcttt cggagccaga agggcagaga aattttggtg gctttaatta ccaagcctat
     9661 ctgaagactc agggaattta ccagactctc aatatcaaaa caatccagtc acttcaaaag
     9721 attggcagtt gggatatagg agaaaacttg tccagtttac gtcgaaaggc tgtggtttgg
     9781 attaagacgc actttccagg ccctatgcgc aattacatga caggactctt gctgggacat
     9841 ctggacaccg actttgagga gatgaatgag ctttattcca gtctaggaat tatccacctc
     9901 tttgccctat ctggcatgca ggtaggtttt ttcatgaatg gatttaagaa acttctcttg
     9961 cgattgggct tgacccaaga aaagttgaaa tggctgactt atcccttttc ccttatctat
    10021 gcgggactaa ctggattttc agcatcggtt attcgcagtc tcttgcaaaa gctactggct
    10081 caacatgggg ttaagggctt ggataatttt gccttgacgg tgcttgtcct ctttattgtc
    10141 atgccaaact ttttcttgat agcaggagga gtcttgtcct gcgcttatgc ttttatcctg
    10201 accatgacca gcaaagaagg ggaggggctc aaggctgttg ctagtgaaag tctagtcatc
    10261 tccttgggca tattgcccat tctatccttc tattttgcgg aatttcaacc ttggtctatc
    10321 cttttgacct ttgtcttttc ctttctattt gacttgacct tcttaccgct cttgtctatt
    10381 ttatttgtcc tttcctttct ctatccagtc attcagctga actttatctt tgaatggtta
    10441 gagggcatta ttcgcttggt ctcgcaggtg gcaaggagac cgcttgtctt tggtcaaccc
    10501 aacgcatggc ttttaatctt attgttaatt tccttggctt tggtctatga tttgaggaaa
    10561 aacattaaag gattaacagt attgagttta ttgattacag gtctcttttt ccttaccaag
    10621 tatccactgg aaaatgaaat caccatgctg gatgtggggc aaggagaaag tattttccta
    10681 cgggatgtaa ctgggaaaac cattctcata gatgtaggtg gtaaggcaga atcttataag
    10741 aaaatcgaaa aatggcaaga aaagatgacg accagcaatg cccagcgaac cttgattccc
    10801 tatctcaaaa gtcgaggagt agctaagatt gaccagctaa ttttgactaa cacggacaag
    10861 gagaatgttg gagatttgtc agaggtgacc aaggctttcc atgtagggga gattctagta
    10921 tcaaaagaca gtctgaaaca gaaggaattt gtggcagaac tacaggcgac tcaaacaaag
    10981 gtgcgtagta tgacagtagg ggagaacttg cccatttttg gaagtcagtt agaagttcta
    11041 tctccaagga aaatgggaga tggaggacac gatgataccc tagttctgta tgggaaattc
    11101 ttggataagc aatttctctt cacgggaaat ttggaggaga aaggagagaa ggacttgctg
    11161 aagcactatc cagacttgaa agtaaatgtt ttgaaagcta gccaacatgg caataaaaaa
    11221 tcatcaagtc cagcctttct agaaaaactc aaaccagagc ttactcttat ctcagttgga
    11281 aagagcaatc gaatgaaact cccccatcag gaaacattga cacgactgga aggtatcaat
    11341 agcaaagttt atcgaactga ccagcaagga gctatacgtt ttaaggggtt ggatagttgg
    11401 aaaatcgaaa gtgttcgata ggaaggataa atgttgtaga ttagtgaaat aaactaaaaa
    11461 tttgttgcat aataatgata aaaatggtat aatgaaaacg tattcaatat tgaggatgta
    11521 aaatcattaa aaattagcaa aaatcgttta attagttagt ttataatcta ttggtcttct
    11581 tcagtccagt gtatctgctg tgacagtcac taaaagttac aagtatgatt ggaatacggt
    11641 ttgggaatat agtaccaact atcacgacca tcagtatgct tggattccgt catggtctcg
    11701 ttatgacagc tattctgagt ataaagttgg cggaggctgg aactacggtc gttatgaggt
    11761 cataaactat tacagcggag gctattaatc cttaaagagt gagaaaagga gggctggata
    11821 tgttaaatat tactcatgtt accttaaaaa cgcgacaagt catcttgcaa gatgtggatt
    11881 tcacctttaa aaagggtagg gtttatggtc ttcttgctat caatggctct ggaaagacga
    11941 ccctgttccg tgccattagc aatttaattc ccataagtag tggaaatatc tcagcccctc
    12001 cttctttatt ttattatgag agtattgaat ggctggatgg aaacttaagt gggatggact
    12061 accttcgtct tatcaaaaac atctggaagt caggtctgaa cttgagggat gaaatcgcct
    12121 attgggaaat gtctgactat atcagtcttc ccattcgcaa gtattcctta ggcatgaagc
    12181 aacgcttggt gattgccatg tatttcctca gtcaggccaa atgctggctc atggatgaga
    12241 ttacaaatgg cttagatgag tattatcgac agaagttttt tgataggtta gcacaaatcg
    12301 atagacaaga acagctggtt cttttaagtt cccactataa ggaagagttg gttgatatct
    12361 gcgatagagt agtaaccatt catcaggggc agatagaaga ggtttagttt atgaaagatg
    12421 ttagtctatt tttattgaaa aaagttttca aaagccgctt aaactggatt atcttagctt
    12481 tatttgtatc tgtactcggt gttacttttt attttaatag tcagactgca aactcagtca
    12541 gcttggagag agagttggaa actcgtcttg tagaccgtga gagagtcatc aatgaatatg
    12601 aagagaaact ctcccaaatg tctgatacca gctcggagga ataccagttt gctaaaaata
    12661 atttagacgt gcaaaaaaat cttttgacgc gaaagacaga aattctaact ttactaaaag
    12721 aagggcgctg gaaagaggcc tactatctgc agtggcaaga tgaagagaaa gattatgaat
    12781 ttgtatcaaa tgacccgact gctagctctg aattaaaaat gggggttgac cgcgaacgga
    12841 agatttatca agccctgtat cccttgaaca taaaagcaca tactttggag tttccgaccc
    12901 acgggattga tcagattgtc tggattttag aggttatcat cccaagcttg tttgtggttg
    12961 cgattatttt tatgctaaca caactatttg cagaaagata tcaaaatcat ctggacacag
    13021 ctcacttata tcctgtttca aaagtgacat ttgcaatatc ctctcttgga gttggagtgg
    13081 gatatgtaac tgtgctgttt atcggaatct gtggcttttc ttttctagtg ggaagtctga
    13141 taagtggttt tggacagtta gattatccct acccaattta tagcttagtg aatcaagaag
    13201 taactattgg gaaaatacaa gatgtattat ttcctggctt gctcttagct ttcttagcct
    13261 ttatcgtcat tgtggaagtt gtgtacttga ttgcttactt tttcaagcaa aaaatgcctg
    13321 tcctctttct ttcactcatt gggattgttg gcttattgtt tggcatccaa acaataagcc
    13381 tcttcaaagg attgcacatc tgattccctt tacttacttg cgttcagtgg agattttatc
    13441 tggaagatta tctaagcaga ttgataatgt cgatctaaat tggagcatgg ggatggtctt
    13501 acttccttgc ctgattatcc ttttgttagt ggggattcta tttatcgaaa gatggggaag
    13561 ttcacgtaaa aaggaagttt ttaaagttta gttttcctat agggaagcta aagaaaaact
    13621 aacataggga gagaatcaac ttggttctct ctttttgatt cgaaaaccaa accaaaatac
    13681 aaacacaaac ttttcaaaaa ataacttttt atcttgacaa gagctagaaa acttggtatc
    13741 atataaaagt tgagaaaagc agaagtgaga gcttctcgcc ttgtgacatt aagttgcctg
    13801 gccctacgga tgaaaagttt cgaagaaacg ctatcataac gtgcgggctt gtatatttac
    13861 gagtccgcta ttgtttttct ctaataaaac aaaagaggtg aaaaccatag caaagcaaga
    13921 cttattcatc aatgatgaga ttcgtgtacg tgaagttcgc ttgattggtc ttgagggaga
    13981 acagctaggc atcaagccac tcagtgaagc gcaagctttg gctgataacg ctaatgttga
    14041 cctagtattg attcaacccc aagccaaacc gcctgttgca aaaattatgg actacggtaa
    14101 gttcaaattt gagtaccaga agaagcaaaa agaacaacgt aaaaaacaaa gcgttgttac
    14161 tgtgaaagaa gttcgtctaa gtccaacaat tgacaagggt gactttgata caaaacttcg
    14221 taatgcacgt aagttccttg aaaaaggaaa taaagttaag gtatctattc gctttaaggg
    14281 tcgtatgatt acccataaag agattggtgc aaaagtttta gccgagtttg ctgaagcaac
    14341 tcaagatatt gccatcatcg aacaacgtgc taaaatggat ggacgtcaaa tgttcatgca
    14401 attggcgcca gcgactgaca aaaaataatt gtcagaaagt taaataaagg agaaaaaatc
    14461 atgccaaaac aaaaaacaca ccgcgcatca gctaaacgtt tcaaacgtac aggttctggt
    14521 ggacttaaac gtttccgtgc ttacacttct caccgtttcc acggaaaaac taagaaacaa
    14581 cgtcgtcatc ttcgtaaagc atctatggtg cattcaggag attacaaacg tatcaaagca
    14641 atgcttactc gcttgaaata atagcttgac tgtaagtaaa caattctagg aaatatttgg
    14701 aggaaataaa acatggcacg tgttaaaggt ggcgttgtat cacgcaaacg tcgtaaacgt
    14761 attcttaaat tagcaaaagg ttactatgga gctaaacaca tcttgttccg tactgcaaaa
    14821 gaacaagtaa tgaactctta ctactatgca taccgtgacc gtcgtcagaa aaaacgtgac
    14881 ttccgcaaat tgtggattac tcgtatcaat gcggcagctc gtatgaacgg actttcatac
    14941 tcacaattga tgcatggttt gaaattggct gagatcgaag ttaaccgtaa aatgcttgct
    15001 gacttggctg ttaacgatgc agtagctttc acagctcttg cagatgcagc taaagcaaaa
    15061 cttggtaaat aacaactgat gagactggaa caggattgtc cagtcttttt aaaaataaag
    15121 gagaaagata tggcttcaaa aatgctacac acttgcttac gagtagaaaa tcttgaaaaa
    15181 tcaatcgcat tttatcaaga tgcttttggt tttaaagaat tgcgtcgcag agattttcca
    15241 gaccatgcct tcacgattgt ctatctaggt cttgagggtg atgattatga gttggagttg
    15301 acttataact acgatcacgg tccgtatgtg gttggtgatg ggtttgccca tatcgccctc
    15361 agtacacctg atcttgaggc acttcatcaa gaacacagtg caaaaggcta tgaagtgact
    15421 gagccaaatg gtctaccagg aactacacca aactattact ttgttaaaga ccctgatgga
    15481 tataaggtcg aagtcattcg tgaaaaataa tttcgtgcaa gttaagtaga atattcgttt
    15541 tctacttgac tttttttagc tgaaagagta tactaataaa aattcaacct ttaaggggag
    15601 tccagagaga ctcacaaggt gtcagataaa agaatagtgc aattttctag aggagacctt
    15661 ttgagtgtgc tctcttgtgt tgtacgattt taactgaggc cttgcactag caaggtcttt
    15721 tctttatctg gtccccttaa aatttaagga ggaaaagtta tgaatctcac atgtaagaag
    15781 cgtttgggtg tcattcggtt ggaaaccatg aaggtggttg cacaagagga aatcgcgcca
    15841 gcaatctttg aattagtcct agaaggagaa atggttgaag ccatgcgagc aggccaattt
    15901 cttcatctgc gtgtaccaga cgatgcccat ctcttacgtc gtcctatttc aatttcgtct
    15961 attgacaagg caaacaagca gtgtcacctc atttatcgga ttgagggagc tgggactgca
    16021 attttttcaa ccttaagtca gggagacact cttgatgtga tggggcctca gggaaatggt
    16081 tttgacttgt ctgaccttga tgagcagaat caggttctcc ttgttggtgg tgggattggt
    16141 gttccaccct tgcttgaggt ggccaaggaa ttgcatgaac gtggagtgaa agtagtgaca
    16201 gtcctcggtt ttgctaataa ggatgctgtt attttgaaaa cggaattggc tcagtatggt
    16261 caggtctttg taacgacaga tgatggttct tatggcatca agggaaatgt ttccgttgtt
    16321 atcaatgatt tagatagtca gtttgatgct gtttactcgt gtggggctcc aggaatgatg
    16381 aagtatatca atcaaacctt tgatgatcac ccaagagcct atttatctct ggaatctcgt
    16441 atggcttgtg ggatgggagc ttgctatgcc tgtgttctaa aagtaccaga aagcgagacg
    16501 gtcagccaac gcgtctgtga agatggtcct gttttccgca caggaacagt tgtattataa
    16561 ggagaaaatt atgactacaa atcgattaca agtgtctcta cctggtttgg atttgaaaaa
    16621 tccgattatt ccagcatcag gctgttttgt ctttggacaa gagtatgcca agtactatga
    16681 tttagacctt ttaggttcta ttatgatcaa ggcgacaacc cttgaaccac gttttgggaa
    16741 tccaactcca agagtggcag agacgcctgc tggtatgctc aatgcaattg gcttgcaaaa
    16801 tcctggttta gaggttgttt tggctgaaaa gctaccttgg ctggaaagag aatatccaaa
    16861 tcttcctatt attgccaatg tagctggttt ttcaaaacaa gagtatgcag ctgtttctca
    16921 tgggatttcc aaggcaacta atgtaaaagc tatcgagctc aatatttctt gtcccaatgt
    16981 tgaccactgt aatcatggac ttttgattgg tcaagatcca gatttggctt atgatgtggt
    17041 gaaagcagct gtggaagcct cagaagtgcc agtttatgtc aaattaaccc cgagtgtgac
    17101 cgatatcgtt actgtcgcaa aagctgcaga agatgcggga gcaagtggct tgaccatgat
    17161 caatactctg gttggaatgc gctttgacct caaaaccaga aaaccaatct tggccaatgg
    17221 aacaggtgga atgtcaggtc cagcagtctt tccagtagcc ctcaaactca tccgccaagt
    17281 agcccaaaca acagacctgc ctatcattgg aatgggggga gtggattcgg ctgaagctgc
    17341 cctagaaatg tatctggctg gggcatctgc tatcggagtt ggaacagcta actttaccaa
    17401 tccttatgcc tgccctgaca tcatcgaaaa tttaccaaaa gtcatggata aatacggtat
    17461 tagcagtctg gaagaactcc gtcaggaagt aaaagagtct ctgaggtaaa ctgcaatcaa
    17521 tctgttcttg attttttatt agtttgtaat atgaatttag gagaattttg gtacaataaa
    17581 ataaataaga acagaggaag aaggttaatg aagaaagtaa gatttatttt tttagctctg
    17641 ctatttttct tagctagtcc agagggtgca atggctagtg atggtacttg gcaaggaaaa
    17701 cagtatctga aagaagatgg cagtcaagca gcgaatgagt gggtttttga tactcattat
    17761 caatcttggt tctatataaa agcagatgct aactatgctg ataatgaatg gctaaagcaa
    17821 ggtgacgact atttttacct caaatctggt ggctatatgg ccaaatcaga atgggtagaa
    17881 gacaagggag tcttttatta tcttgaccaa gatggaaaga tgaaaagaaa tgcttgggta
    17941 ggaacttcct atgttggtgc aacaggtgcc aaagtaatag aagactgggt ctatgattct
    18001 caatacgatg cttggtttta tatcaaagca gatggacagc acgcagagaa agaatggctc
    18061 caaattaaag ggaaggacta ttatttcaaa tccggtggtt atctactgac aagtcagtgg
    18121 attaatcaag cttatgtgaa tgctagtggt gccaaagtgc agcaaggttg gctttttgac
    18181 aaacaatacc aatcttggtt ttacatcaaa gaaaatggaa actatgctga taaagaatgg
    18241 attttcgaga atggtcacta ttattatcta aaatccggtg gctacatggc agccaatgaa
    18301 tggatttggg ataaggaatc ttggttttat ctcaaatttg atgggaaaat agctgaaaaa
    18361 gaatgggtct acgattctca tagtcaagcc tggtactact tcaaatccgg tggttacatg
    18421 acagccaatg aatggatttg ggataaggaa tcttggtttt acctcaaatc tgatgggaaa
    18481 atagctgaaa aagaatgggt ctacgattct catagtcaag cttggtacta cttcaaatcc
    18541 ggtggttaca tgacagccaa tgaatggatt tgggataagg aatcttggtt ttacctcaag
    18601 tctgatggca aaatggctga aaaagaatgg gtctacgatt ctcatagtca agcctggtac
    18661 tacttcaaat ctggtggcta catggcgaaa aatgagacag tagatggtta tcagcttgga
    18721 agcgatggta aatggcttgg aggaaaagct acaaatgaaa atgctgctta ctatcaagta
    18781 gtgcctgtca cagccaatgt ttatgattca gatggtgaaa agctttccta tatatcgcaa
    18841 ggtagtgtcg tatggctaga taaggataga aaaagtgatg acaagcgctt ggctattact
    18901 atttctggtt tgtcaggcta tatgaaaaca gaagatttac aagcgctaga tgctagtaag
    18961 gactttatcc cttattatga gagtgatggc caccgttttt atcactatgt ggctcagaat
    19021 gctagtatcc cagtagcttc tcatctttct gatatggaag taggcaagaa atattattcg
    19081 gcagatggcc tgcattttga tggttttaag cttgagaatc ccttcctttt caaagattta
    19141 acagaggcta caaactacag tgctgaagaa ttggataagg tatttagttt gctaaacatt
    19201 aacaatagcc ttttggagaa caagggcgct acttttaagg aagccgaaga acattaccat
    19261 atcaatgctc tttatctcct tgcccatagt gccctagaaa gtaactgggg aagaagtaaa
    19321 attgccaaag ataagaataa tttctttggc attacagcct atgatacgac cccttacctt
    19381 tctgctaaga catttgatga tgtggataag ggaattttag gtgcaaccaa gtggattaag
    19441 gaaaattata tcgatagggg aagaactttc cttggaaaca aggcttctgg tatgaatgtg
    19501 gaatatgctt cagaccctta ttggggcgaa aaaattgcta gtgtgatgat gaaaatcaat
    19561 gagaagctag gtggcaaaga ttagtactat aagtgaatat gatttgagtg aatagtaagt
    19621 taaaaatcct gatttcaagt aaaatcagga ttttttcatg gatgcaattt ttttggagtc
    19681 tggtgtgacg cggagggtct tttgtcctgt gtaagtgaca aagccgggtt ttccaccagt
    19741 tggtttattg agttttttga cttcaatcat atctacctgc accagattcg acaggcgccc
    19801 ttgagagaag taggcagcta actctgctgc gtctgtcttg actgcatcag atgggtcaag
    19861 atttcctgag atgacaacat ggcttccagg aatgtcctta gcatggaacc aaagttcctc
    19921 cttgcgggcc attttaaagg tcaattcctc attttgaaga ttgtttcgtc cgacatagat
    19981 gatggttttg ccatcgcttg ctagatattg ttctagtttt ttgcgtttct ggattttctc
    20041 ccgttgtctt ctgcggataa aacctgtttg aatcaattct tcacggattt cagcgatttc
    20101 ttccagtcca gcttggttga ggacggtttc tacactttcc agatagagaa tagtggcttt
    20161 ggtttcttca atcaaatcag tcaagtattt gacagcttct ttgagtttct gataccgttt
    20221 aaaatagcgt tgggcattct ggttgggagt cagagcctta tcaagcgcaa tcatgatagg
    20281 ttggttggta tagtagttgt ctaggataac ctggtcttgg tcgttaggca cttggtggag
    20341 gaaggttgtc agcaattctc ctttttgacg aaattcttca gcgttgtctg tcgccagtaa
    20401 ctctttttcc tgttttttga gtttgtgtcg gtttttctga agttcatttt caacacgacg
    20461 aatcagttca ctggcctgct gtttgacgcg gtcgcgctca gccttatcct tatagtaggt
    20521 gtccaacaaa tcagaaagat ttgcaaaagg ctctcccacc tgatttgcaa aaggaactgg
    20581 actgaaggaa gtctcagtca agcatggctt ggtttcttga ttgaaaaaat ttcggaaagc
    20641 ggaaagtttt tcactaacca gtatcctttc caattcattt gccgtatcgc gtcccagacc
    20701 ttgaaagagg ctttgaagat tttttgctgt tagttcttgg gtttgtagga tttcaaagag
    20761 cttttcatcc ttgatagtaa aaggattgag agattttgta cttggcggag cgatataggt
    20821 cgatcctgga agtaaggtgc ggtagctatt ttgtgaaaaa ccgacgtgtt tgataacttc
    20881 gaggatttta tgactgcttt tatcgaccag tagaatatta ctgtgttttc ccataatttc
    20941 aataatcaag gtagcctgga tatggtctcc aatctcgttt ttattggaaa ctgtaatttc
    21001 cacaatacgg tcattttcca cttgctcaat cgactcaatc agggccccct gcaaatactt
    21061 tctcaaaacc atgataaagg tagaaggttg agctggattt tcaaaagtcg tttgggtcag
    21121 ctgaatgcgt ccaaaaactg gatgggcaga aaggagcagg cgatggcttt ggcgattgct
    21181 gcggatttgc aagaccaact cttgttcaaa aggctgattg attttctgga tgcgaccatt
    21241 cactaattcg cttcgcaatt cctcaactat gtggtgtaaa aaaaatccgt caaatgacat
    21301 cgttctctcc ttgtgattgt attccatagt attatatcaa aaaggtagaa taaaatcatg
    21361 gaaatgtggt ataataaagc caagtaaaga gaaacgagaa gcacatgtat attgaaatgg
    21421 tagatgaaac tggtcaagtt tcaaaagaaa tgttgcaaca aacccaagaa attttggaat
    21481 ttgcagccaa aaaattagga aaagaagaca aggagatggc agtcactttt gtgaccaatg
    21541 agcgtagtca tgaacttaat ctggagtacc gtgacaccga ccgtccgaca gatgtcatca
    21601 gccttgagta taaaccagaa ttggaaattg cctttgacga agaggatttg cttgaaaatc
    21661 cagaattggc agagatgatg tctgagtttg atgcctatat tggggaattg ttcatctcta
    21721 tcgataaggc tcatgagcag gccgaagaat atggtcacag ctttgagcgt gagatgggct
    21781 tcttggcagt acacggcttt ttacatatta acggctatga tcactatact ccggaagaag
    21841 aagcggagat gttcggttta caagaagaaa ttttgacagc ctatggactc acaagacaat
    21901 aaacgaaaat ggaaaaatcg tgacttgata tccagtttag aatttgccat cacagggatt
    21961 tttactgcta tcaaggaaga acgaaatatg cgaaaacatg cagtgacggc tctagtggtc
    22021 atccttgcag gttttgtttt tcaggtgtca cgaatcgaat ggctctttct cctattgagt
    22081 attttcttgg tagtagcctt tgagattatc aactctgcta ttgaaaatgt ggtggatttg
    22141 gccagtcact atcacttttc catgctggct aaaaatgcca aggatatggc ggccggcgcg
    22201 gtattagtgg tttctctttt cgcagcctta acaggcgcat tgatttttct cccacgaatc
    22261 tgggatttat tattttaaac agtaagagga aattatgact tttaaatcag gctttgtagc
    22321 cattttagga cgtcccaatg ttgggaagtc aaccttttta aatcacgtta tggggcaaaa
    22381 gattgccatc atgagtgaca aggcgcagac aacgcgcaat aaaatcatgg gaatttacac
    22441 gactgataag gagcaaattg tctttatcga cacaccaggg attcacaaac ctaaaacagc
    22501 tctcggagat ttcatggttg agtctgccta cagtaccctt cgcgaagtgg acaccgttct
    22561 tttcatggtg cctgctgatg aagcgcgtgg taagggggac gatatgatta tcgagcgtct
    22621 caaggctgcc aaggttcctg tgattttggt ggtgaataaa atcgataagg tccatccaga
    22681 ccagctcttg tctcagattg atgacttccg taatcaaatg gactttaagg aaattgttcc
    22741 aatctcagcc cttcagggaa ataacgtgtc tcgtctagtg gatattttga gtgaaaatct
    22801 ggatgaaggt ttccaatatt tcccgtctga tcaaatcaca gaccatccag aacgtttctt
    22861 ggtttcagaa atggttcgcg agaaagtctt gcatctaact cgtgaagaga ttccgcattc
    22921 tgtagcagta gttgttgact ctatgaaacg agacgaagag acagacaagg ttcacatccg
    22981 tgcaaccatc atggtcgagc gcgatagcca aaaagggatt atcatcggta aaggtggcgc
    23041 tatgcttaag aaaatcggta gcatggcccg tcgtgatatc gaactcatgc taggagacaa
    23101 ggtcttccta gaaacctggg tcaaggtcaa gaaaaactgg cgcgataaaa agctagattt
    23161 ggctgacttt ggctataatg aaagagaata ctaagtagag gtaggctcat gcctgcttct
    23221 tgtttttaca gaaggaggac ttatgcctga attacctgag gttgaaaccg tttgtcgtgg
    23281 cttagaaaaa ttgattatag gaaagaagat ttcgagtata gaaattcgct accccaagat
    23341 gattaagacg gatttggaag agtttcaaag ggaattgcct agtcagatta tcgagtcaat
    23401 gggacgtcgt ggaaaatatt tgctttttta tctgacagac aaggtcttga tttcccattt
    23461 gcggatggag ggcaagtatt tttactatcc agaccaagga cctgaacgca agcatgccca
    23521 tgttttcttt cattttgaag atggtggcac gcttgtttat gaggatgttc gcaagtttgg
    23581 aaccatggaa ctcttggtgc ctgacctttt agacgcctac tttatttcta aaaaattagg
    23641 tcctgaacca agcgaacaag actttgattt acaggtcttt caagctgccc ttgccaagtc
    23701 caaaaagcct atcaaatccc atctcctaga ccagaccttg gtagctggac ttggcaatat
    23761 ctatgtggat gaggttctct ggcgagctca ggttcatcca gctagacctt cccagacttt
    23821 gacagcagaa gaagcgactg ccattcatga ccagaccatt gctgttttgg gccaggctgt
    23881 tgaaaaaggt ggctccacca ttcggactta taccaatgcc tttggggaag atggaagcat
    23941 gcaggacttt catcaggtct atgataagac tggtcaagaa tgtgtacgct gtggtaccat
    24001 cattgagaaa attcaactag gcggacgagg cacccacttt tgtccaaact gtcaaaggag
    24061 ggactgatga gaaaaatcat cggaatcact gggggaattg cctctggtaa gtcaactgtg
    24121 acaaattttc taagacagca aggctttcaa gtagtggatg ccgacgcagt cgtccaccaa
    24181 ctacagaaat ctggtggtcg tctgtttgag gctctagtac agcactttgg gcaagaaatc
    24241 attcttgaaa acggagaact caatcgccct ctcctagcta gtctcatctt ttcaaatcct
    24301 gaagagcaaa aatggtctaa gcaaattcaa ggggagatta tccgtgagga actggctact
    24361 ttgagagaac agttggctca gacagaagag attttcttca tggatattcc cctacttttt
    24421 gagcaggact acagcgattg gtttgctgag acttggttgg tctatgtgga ccgagatgcc
    24481 caagtagaac gcttaatgaa aagggaccag ttgtccaaag atgaagctga gtctcgtctg
    24541 gcagcccagt ggcctttaga aaaaaagaaa gatttggcca gccaggttct tgataataat
    24601 ggcaatcaga accagcttct taatcaagtg catatccttc ttgagggagg taggcaagat
    24661 gacagagatt aactggaagg ataatctgcg tattgcctgg tttggtaatt ttctgacagg
    24721 agccagtatt tctttggttg taccttttat gcccatcttc gtggaaaatc taggtgtagg
    24781 gagtcagcaa gtcgcttttt atgcaggctt agcaatttct gtctctgcta tttccgcggc
    24841 gctcttttct cctatttggg gtattcttgc tgacaaatac ggccgaaaac ccatgatgat
    24901 tcgggcaggt cttgctatga ctatcactat gggaggcttg gcctttgtcc caaatatcta
    24961 ttggttaatc tttcttcgtt tactaaacgg tgtatttgca ggttttgttc ctaatgcaac
    25021 ggcactgata gccagtcagg ttccaaagga gaaatcaggc tctgccttag gtactttgtc
    25081 tacaggcgta gttgcaggta ctctaactgg tccctttatt ggtggcttta tcgcagaatt
    25141 atttggcatt cgtacagttt tcttactggt tggtagtttt ctatttttag ctgctatttt
    25201 gactatttgc tttatcaagg aagattttca accagtagcc aaggaaaagg ctattccaac
    25261 aaaggaatta tttacctcgg ttaaatatcc ctatcttttg ctcaatctct ttttaaccag
    25321 ttttgtcata caattttcag ctcaatcgat cggccctatt ttggctcttt atgtacgcga
    25381 cttagggcag acagagaatc ttctttttgt ctctggtttg attgtgtcca gtatgggctt
    25441 ttccagcatg atgagtgcag gagtcatggg caagctaggt gacaaggtgg gcaatcatcg
    25501 tctcttggtt gtcgcccagt tttatttagt catcatctat ctcctctgtg ccaatgcctc
    25561 tagcccgctt caactaggac tttatcgatt cctctttgga ttgggaaccg gtgccttgat
    25621 tcccggggtt aatgccctac tcagcaaaat gactcccaaa gccggcattt cgagggtctt
    25681 tgccttcaat caggtattct tttatctggg aggtgttgtt ggtcccatgg caggttctgc
    25741 agtagcaggt caatttggct accatgctgt cttttatgcg acgagccttt gtgttgcctt
    25801 tagttgtctc tttaacctga ttcaatttcg aacattatta aaagtaaagg aaatctagtg
    25861 cgagtaaaaa ttaatctcaa atgctcctct tgtgacagta tcaattacct aaccagtaaa
    25921 aattcaaaaa cccatccaga caagattgag gttttaaagt attgtcccaa ggaaagaaaa
    25981 gtaaccctac atcttgaatc taagtagatt ttatggtaaa ataataggga ttttaaggag
    26041 tttgatatgt ataacctatt attaaccatt ttattagtat tatctgttgt gattgtgatt
    26101 gcaattttca tgcaaccaac caaaaaccaa tccagcaatg tatttgatgc cagttcaggt
    26161 gatttgtttg aaagcagtaa agctcgcggt tttgaagctg taatgcagcg tttgacaggg
    26221 attttagtct ttttctggct agccattgcc ttagcattga cggtattatc aagtagataa
    26281 gaaaataatg ggcaggacta ggtctttgcc tctttttatt tttaaaggat gtttgagaag
    26341 gttttacagt aaaagaaaat taaaaaatct agaaagaaaa tatgaaagat agaataaaag
    26401 aatatttaca agacaaggga aaggtgactg ttaatgattt ggctcaggct ttgggaaaag
    26461 acagttccaa ggattttcgt gagttgatta aaaccttgtc cttaatggaa agaaagcacc
    26521 agattcgctt tgaagaagat ggcagtctga ccttggaagc caagaaaaaa catgagatta
    26581 ccctcaaggg gatttttcat gcccataaaa atggttttgg ttttgtcagc ctagaagggg
    26641 aagaggacga cctttttgta gggaaaaatg atgtcaacta tgctattgat ggtgataccg
    26701 tcgaggtagt gattaagaaa gtcgctgacc gcaataaggg aacagcagca gaagccaaaa
    26761 ttattgatat cctagaacac agtttgacaa cagttgtcgg gcaaatcgtt ctggatcagg
    26821 aaaagcctaa gtatgctggc tatattcgtt caaaaaatca gaaaatcagt caaccgattt
    26881 atgttaagaa accagcccta aaattagaag gaacagaagt tctcaaagtc tttatcgata
    26941 aatacccaag caagaaacat gatttctttg tcgctagtgt actcgatgta gtgggacact
    27001 caacggatgt cggaattgat gttcttgagg tcttggaatc aatggacatt gtatccgagt
    27061 ttccagaagc tgttgttaag gaagcagaaa gtgtgcctga tgctccgtct caaaaggata
    27121 tggaaggtcg tctggatcta agagatgaaa ttacctttac cattgacggt gcggatgcca
    27181 aggacttgga cgatgcagtg catatcaagg ctctgaaaaa tggcaatctg gagcttgggg
    27241 ttcacattgc agatgtttct tattatgtga ccgaggggtc tgcccttgac aaggaagccc
    27301 ttaaccgtgc gacttctgtt tacgtgacag accgagtggt gccaatgctt ccagaacgac
    27361 tatcaaatgg catctgctct ctcaatcccc aagttgaccg cctgacccag tctgctatta
    27421 tggagattga taaacatggt cgtgtggtca actataccat tatacaaaca gttatcaaga
    27481 ccagttttcg tatgacctat agcgatgtca atgatatcct agctgtcgat gaagaaaaga
    27541 gaaaagaata tcataaaatt gtatcaagta tcgaactcat ggccaagctt catgaaactt
    27601 tagaaaacat gcgtgtgaaa cgtggagctc tcaattttga taccaatgaa gcgaagattt
    27661 tagtggataa acaaggtaag cctgttgata tcgttcttcg gcagcgtggc attgccgagc
    27721 ggatgattga gtcttttatg ttgatggcta atgaaacagt tgccgaacat ttcagcaagt
    27781 tggatttgcc ttttatctat cgaattcacg aggagcctaa ggctgaaaag gttcagaagt
    27841 ttattgatta tgcttcgagt tttggcttgc gtatttatgg gactgccagt gagattagtc
    27901 aggaggcact tcaagacatc atgcgtgctg ttgagggaga accttatgca gatgtattgt
    27961 ccatgatgct tcttcgctct atgcagcagg ctcgttattc ggagcacaat cacggccact
    28021 atggactagc tgctgactat tatactcact ttaccagtcc aattcgtcgt tatccagacc
    28081 ttcttgttca ccgtatgatt cgggattacg gccgttctaa ggaaatagca gagcattttg
    28141 aacaagtgat tccagagatt gcgacccagt cttccaaccg tgaacgtcgt gccatagaag
    28201 ctgagcgtga agtcgaagcc atgaaaaagg ctgagtatat ggaagaatac gtgggtgaag
    28261 agtatgatgc agttgtatca agtattgtca aattcggtct ctttgtcgaa ttgccaaaca
    28321 cagttgaagg cttgattcac atcactaatc tacctgaatt ttatcatttc aatgagcgtg
    28381 atttgactct tcgtggagaa aaatcaggta tcactttccg agtgggtcag cagatccgta
    28441 tccgtgttga aagagcggat aaaatgactg gagagattga tttttcattc gtacctagtg
    28501 agtttgatgt gattgaaaaa ggcttgaaac agtctagtca tagtggcaga gggcgtggtt
    28561 caaatcgtcg ttcggataag aaggaagaca agagaaaatc aggacgctca aatgataagc
    28621 gtaagcattc acaaaaagac aagaagaaaa aaggaaagaa acctttttac aaggaagtag
    28681 ctaagaaagg agccaagcat ggcaaagggc gagggaaagg tcgtcgcaca aaataaaaag
    28741 gcacgccacg actatacaat cgtagatacg ctagaggcag ggatggtcct gactggaact
    28801 gaaatcaaga gtgtacgagc tgctcgaatt aatctcaagg atggctttgc tcaagtgaaa
    28861 aatggagaag tttggctgag caatgttcat atcgcgcctt acgaagaggg caatatctgg
    28921 aaccaggaac cagaacgtcg tcgtaaactc ctgctccata aaaagcaaat tcaaaaattg
    28981 gaacaagaga ccaaagggac aggaatgacc ttagttcccc ttaaggtcta tataaaagat
    29041 ggctacgcta agcttctttt aggacttgcc aaagggaagc atgactatga caaacgggag
    29101 tctatcaaac gtcgtgagca aaatcgagat atcgcgcgtg tgatgaaagc tgttaatcag
    29161 cgataaaaag aggaattgaa aatggaaaaa ttagttgcct ataaacgcat gcctttgtgg
    29221 aataaacaaa caatgcctga agctgttcag caaaagcaca atacaaaagt tgggacttgg
    29281 gggaaaatta ctgtcttgaa gggagctctc aagtttattg aattgacaga agaaggggaa
    29341 gttctagctg aacacctctt tgaagcaggg gcagacaatc caatggccca acctcaagcc
    29401 tggcaccgag tggaagctgc cacagatgat gtggaatggt acttggaatt ttattgtaaa
    29461 cctgaggatt attttgctaa aaaatacaat accaatcctg ttcattcaga ggtcctagag
    29521 gccatgcaga cagtgaaaca agggaaagct ttggatttgg gttgtggtca gggacgtaat
    29581 tctctttttc tagcccagca agattttgat gtgacggctg tagatcaaaa tggactagct
    29641 cttgaaatct tgcaaagcat tgtggagcag gaagatttgg acatgcctgt tggcctttac
    29701 gatatcaatt cagctagcat tgaacaagaa tatgatttta tcgtttcaac agttgttctc
    29761 atgtttctac aagcggaccg cattccagct attattcaaa atatgcagga gaaaaccagt
    29821 gttggtggtt acaaccttat cgtttgtgcc atggacacgg aggattatcc ttgctcggtt
    29881 aacttcccat tcacctttaa agaaggagaa ctggcagact attacaagga ttgggaattg
    29941 gttaagtaca atgaaaatcc aggccatttg catcgtcgcg atgagaatgg caatcgtatt
    30001 caactacgct ttgcgacctt actagctaag aaaatcaagt aaacacacat gaagattagg
    30061 aattttcctg atcttttttc ttttttacga atgatataga aaaggaggga attcatgttt
    30121 gttgcgagag atgctagggg agaattggta aatgtgttag aggataaact tgagaagcaa
    30181 gcatacacct gcccagcttg tggaggccag atccatttgc gtcaaggacc aagtgtacgg
    30241 acgcattttg cccataaatc cttaaaagac tgtgattttt tctttgaaaa tgaaagtcca
    30301 gaacacatag ccaataagga atccctctat cactggttga aaaaagagac aaaggttcaa
    30361 ttagagtacc cgctttcaga acttaaacag attgcggatg tatttgtaaa tggcaatcta
    30421 gctctagaag ttcagtgtag tcccttgcct cagaaagtcc ttaaagagcg aagtgagggc
    30481 tatcgtagtc agggttacca agtactgtgg ttgctgggta aaaaactgtg gctcaaggag
    30541 cgtttgactt gtctacagca aggttttctt tatttcagtc aaaacatggg cttttatgtt
    30601 tgggaattag acaaggaaaa acaagtttta agactcaaat acctgattta ccaggatctc
    30661 cgcggtaaac tccattatca aatcaaggaa ttttcctatg gtcaaggtag tttattggaa
    30721 atattgcgtc ttccctataa gagacaaaaa atatctcatt ttacagtttc tgaggacaag
    30781 gacatctgtc gctatatccg gcaacaactt tattatcaaa atctcttttg gatgaaagaa
    30841 caagcagaag cctatcaaaa gggagaaaat atcctgactt atggactgaa agaatggtat
    30901 ccacaaattc gaccaatagt gggcaaattt ttccagatta aacaagactt gactagctat
    30961 tatcagcact tttataccta ttaccaaaaa aatcctcaaa atgattggca aaagctttat
    31021 ccaccagcct tttatcagca atatttcttg aaaaatatgg tagaatagaa aggatggagg
    31081 aatctaatgg tattacaaag aaatgaaata aatgaaaaag atacatggga tctatcaacg
    31141 atctacccaa ctgaccaggc ttgggaagaa gccttaaaag atttaacaga acaattggag
    31201 acagtagccc agtatgaagg ccatctcttg gatagtgcgg ataacctact agaaatcact
    31261 gaattttctc ttgaaatgga acgccagatg gagaagcttt acgtttatgc tcatatgaag
    31321 aatgaccagg atacacgtga agctaagtat caagagtact atgccaaggc catgacactc
    31381 tacagccagt tagaccaagc cttttcattc tatgagcctg aatttatgga gattagcgaa
    31441 aagcagtatg ctgacttttt agaagctcaa ccaaagctgc aggtttatca acactatttt
    31501 gacaagctct tgcaaggcaa ggatcacgtt ctctcacaac gtgaagaaga attattggct
    31561 ggagctggag aaatctttgg ttcagcaagt gaaaccttcg ctatcttgga caatgcggat
    31621 attgtgttcc cttatgtcct agacgatgat ggtaaagaag ttcagctatc tcatgggact
    31681 tacacacgtt tgatggagtc taaaaaacgt gaggttcgcc gtggtgccta tcaagctctt
    31741 tatgcgactt acgaacaatt ccaacacacc tatgccaaaa ccttgcaaac caatgttaag
    31801 gtgcaaaatt accgtgctaa agttcgtaac tacaagagtg ctcgtcatgc agccctcgca
    31861 gcgaattttg ttccagaaag tgtttatgac aatttggtag cagcagttcg caagcatttg
    31921 ccactcttac atcgctatct tgagcttcgt tcaaaaatct tggggatttc agatctcaag
    31981 atgtacgatg tttacacacc gctttcatct gttgaataca gttttaccta ccaagaagcc
    32041 ttgaaaaaag cagaagatgc tttggcagtc ttgggtgagg attacttgag ccgtgttaaa
    32101 cgtgccttca gcgagcgttg gattgatgtt tacgaaaatc aaggcaagcg ttcaggtgcc
    32161 tactctggtg gttcttacga taccaatgcc tttatgcttc tcaactggca ggacaatctg
    32221 gacaatctct ttactcttgt tcatgaaaca ggtcacagta tgcattcaag ctatactcgt
    32281 gaaactcagc cttatgttta cggagattac tctatctttt tggctgagat tgcctcaact
    32341 accaatgaaa atatcttgac ggagaaatta ttggaagaag tggaagacga cgcaacacgt
    32401 tttgctattc tcaataactt cctagatggt ttccgtggaa cagttttccg ccaaactcaa
    32461 tttgctgagt ttgaacacgc cattcaccaa gcagatcaaa atggggaggt cttgacaagc
    32521 gatttcctaa ataaactcta cgcatacttg aaccaagagt attatggttt gagtaaggaa
    32581 gacaatcctg aaatccaata cgagtgggct cgcattccac acttctacta taactactat
    32641 gtatatcaat attcaactgg ctttgcggcc gcctcagcct tggctgaaaa aattgtccat
    32701 ggtagtcaag aagaccgtga ccgctatatc gactacctca aggcaggtaa gtcggactat
    32761 ccacttaatg tcatgagaaa agctggtgtt gatatggaga aggaagacta cctcaacgat
    32821 gcctttgcag tctttgaacg ccgtttaaat gagtttgaag cccttgttga aaaattagga
    32881 ttggcataaa atggttgaat cgtatagtaa gaatgctaac cataacatgc gtcgtcctgt
    32941 cgtcaaagaa gaaattgtag acttgatgcg tcagcgtcaa aagcaagtca caggtttctt
    33001 gaaagaattg gaagactttg cccgcaagga aaatattcct attattcccc atgaaacggt
    33061 tgcttatttc cgttttctta tggaaaccat gcagcctaaa aatattctgg aaattgggac
    33121 ggctatcggt ttttcagctc tcttgatggc tgaacatgcg ccaaatgcta agattacaac
    33181 tattgatcgt aatccagaaa tgattggttt tgccaaggaa aattttgccc agtttgacag
    33241 tcgcaagcaa atcactctcc tagagggaga tgcggtggat gtcttatcta cactgacaga
    33301 gtcttatgat ttcgtcttta tggattctgc caagtctaaa tacatcgtct ttctgccaga
    33361 aatcctcaaa catttggaag ttggtggtgt ggttgtcttg gatgatattt ttcaaggtgg
    33421 tgatgttgcc aaggatatta tggaagtccg tcgtggtcag cgaaccattt atcgaggcct
    33481 tcaaaaatta tttgatgcaa ccttagacaa tccagaactc accgcaacat tagtgccttt
    33541 aggagatggt attctcatgc ttcgtaaaaa tgtagcagat gttcaactgt ctgaaagcga
    33601 atgattttca gaaaaattta agaaaaaata gtaaaataga tagagtaaca cttatctcaa
    33661 aggagtagac atgaagaaaa aattattggc aggtgccatc acactattat cagtagcaac
    33721 tttagcagct tgttcgaaag ggtcagaagg ggcagacctt atcagtatga aaggggatgt
    33781 catcacagaa catcaatttt atgagcaagt gaaaaacaac ccttcagccc aacaagtctt
    33841 gttaaatatg accatccaaa aagtttttga aaaacaatat ggctcagagc ttgatgataa
    33901 agaggttgat gatactattg ccgaagaaaa aaaacaatat ggcgaaaact accaacgtgt
    33961 cttgtcacaa gcaggtatga ctcttgaaac acgtaaagct caaattcgta caagtaaatt
    34021 agttgagttg gcagttaaga aggtagcaga agctgaattg acagatgaag cctataagaa
    34081 agcctttgat gagtacactc cagatgtaac ggctcaaatc atccgtctta ataatgaaga
    34141 taaggccaaa gaagttctcg aaaaagccaa ggcagaaggt gctgattttg ctcaattagc
    34201 caaagataat tcaactgatg aaaaaacaaa agaaaatggt ggagaaatta cctttgattc
    34261 tgcttcaaca gaagtacctg agcaagtcaa aaaagccgct ttcgctttag atgtggatgg
    34321 tgtttctgat gtgattacag caactggcac acaagcctac agtagccaat attacattgt
    34381 aaaactcact aagaaaacag aaaaatcatc taatattgat gactacaaag aaaaattaaa
    34441 aactgttatc ttgactcaaa aacaaaatga ttcaacattt gttcaaagca ttatcggaaa
    34501 agaattgcaa gcagctaata ttaaggttaa ggaccaagcc ttccaaaata tctttaccca
    34561 atatatcggt ggtggagatt caagctcaag cagtagtaca tcaaacgaat agtccaaatc
    34621 aatgagtcag ggaaaaaact cgacttcagg aaaaaatgaa gcaaacattc ccacaataaa
    34681 acgcatagta caaggtttgt actgcccccc aaaaaagtta gacaattaat ttatccgaag
    34741 gatttagttc tgtattgcac agagctaagt ccttttagtt ttatcttaat tctcttattg
    34801 ttgtaataat caatatagtc tataatggct tgttccaatt gattaagtga tttaaatgtt
    34861 tgctcatagc cataaaacat ttcggatttt aaaatgccaa agaaagattc catcctaccg
    34921 ttgtcttggc tgtttccttt gcgtgacata gatgcttgaa ttcccttact ctctaggaag
    34981 cgatgataag aatcgtgttg gtattgccag ccttggtcac tatggagaat cgtattctcg
    35041 tagtgcttct ctttgaatgc ctgttccaac attaacgatc aatcaattta atcatgtacc
    35101 taagattaga attgtttatc ccaaatttat ttgaaagctt ctctaagctt aagctatatc
    35161 cttgttttct aagttcatag atctgaactt tatcatcata agttaatttc ataataaaaa
    35221 caccccaaaa gttagatttt ttctgtctaa cttttggggt gtagttcatg tacacctgat
    35281 atgatgcgtt ttataatttt aaagactttt tgaccagcct cattttttta acttgatact
    35341 cagtgaaaag caaagattaa actaggaagc tagctgtagg ctgctcaaag aacagctttg
    35401 aggttgcaga taaaacttgt gaggtaacca acatatataa tgtgaagctg acgtggtttg
    35461 aatagatttt agaagggtat gagtctggaa gttttaatgg ataatgcaag attccataga
    35521 atgggtaagc tagagttctt atgtgaagag tttgggcata aacttttacc ttttcctccc
    35581 tactcatctt agtatagaaa agtgaatcta aaatagtaca taactgcttc taaaacattc
    35641 ttataaattg atttaaattc tcaaatcata ttattcagtt cttatttcat tttgctctac
    35701 aatcctgttg agaagacacg tgttcatatc aaaaaggtat tggcaagttg caataccttt
    35761 ttacaaggtt cttttgtctt atttttgttt caactgacta tatctcctat ggttctagtt
    35821 cagaaggcta ggctataatt atgattgata agaagtatca ttccaagtat tgagagtgaa
    35881 tgtttcaaaa tcatgggttt ctataatggt caggctggca tttgctagac cgccatcttt
    35941 acgaagaagt ggttctttat agcctaggag agtacgaaga ctggcagtaa gattggcgcc
    36001 gtgtccgaca attagaatac gctcagctgg actatctttt aatgatttga taaattggat
    36061 ggtccgctga gttgtactat agagggattc ggctccgaac attcgagtgt caaattgagc
    36121 aagatttgaa cgaaaagcct ggatttgttg cgggtaaata gcttccaggg ttgcaatttt
    36181 caaaccttct aacttcccaa gttgccattc acggagatta ggaacgattt ctaaagaaca
    36241 gggggtatgg agttgacttt ggataatctc agcagatttg accgctcgag gtaaatcact
    36301 tgaataaatc tgatcaaaag gaatttcctt gagatactgg ccaagtcgtt ttagggtttc
    36361 aatggatcca ggaagaagag gagaatcacc gctagcacct tgaaaacgac cttcttggtt
    36421 ccagagggta cgaccgtggc ggacaaagta gagtttcatt acttgatgtc ctccaaaata
    36481 tctacaaagt ctgcctttac aaagctagcc aagtcttgtg gcgcgacgat aatgctgtgt
    36541 ccgacttcgc ctgcagagac aatcattcga tccaaatcta gagcaatttt atcgataaaa
    36601 atgggataat tgtgtttctg acgaattccg acaggattat tggctccatg aatgtaacca
    36661 gttgtttttt ctaagtcctt ttgtggaatc atgctcattt ttttattgcc agaaatttta
    36721 gctagtttct tttcagacaa gtgctgagtg atagggacaa ttccgataat cggtccggtc
    36781 ttgtctccca aaagcgccaa ggttttgaaa atctgatctc gttcataacc ttgaggaagc
    36841 tctccttcta gggcattgat ttgaatcccc tgatgaggga tagctgcttt agataggatt
    36901 tgttccacca atgttttttt gattttaact ttttttgcca ttatttatat ttatcctcca
    36961 attgactcat ccaaatacca agccagattc ccagcgcaaa gaagaaggcg atgatgacat
    37021 aaccgacaag tgaaagtcct gtgtattgga tactttcagc gtttcctgca tttggaatta
    37081 agatcaaaag ggtacttgat aggacgatac cgatgatgaa atgatagacg cgtgagtggt
    37141 agttgtttaa ggcataatcc atcaattttg aaaaaacgat gagagttgca cctgcaccaa
    37201 ttccaatcgg aaagaaggtt cccaagagat caaaagtttt aaaaccagtc aacataggag
    37261 catagagtcc caaaatcaaa agtaaattcg atgggctgag gccaggaacc aagacgccaa
    37321 gggccaatag tgcgcctgct aggacgaagt taagaaagct ggcgcttaag gttccaacga
    37381 caaaatttaa ggcatagagt cctaatccag aaatgataaa ggttgtccat aaccaagcta
    37441 aatcaatctt gtctcggtca gattctcgag ttgattcttt gaggaggcta ggaactgtac
    37501 cgataatagc tcccgcaaag ctccataata caaagacctg ataattttca agcaggtatt
    37561 caatcgggta ggaaaataag ccgattccca gaagcatacc gatggcaact ggaataaagt
    37621 acaaaacatt ttctttaaag tctttaaagg gatgggccag aaagccaatc attcgttcat
    37681 agattcctaa gattgctgct agaacccctc cggaaattcc cggtaggata aatccaagag
    37741 caattacaat ccctttaata acgcgtgcta accatgagag catatatttc ctccaactat
    37801 ttctatttca aaaaatcctt actatattgt atcacaatca gacacaaaaa gaaaaagcaa
    37861 atgataaaca aatgctttta aggttttaaa aaaagttttc gaaaggtttc ttctttattt
    37921 tttaagggag agataacgtt gatatctaaa tcgtggtcaa agccggcaat ttttccttta
    37981 gatgtgtatt ggtgaatatc ataatctaaa tcagttttag gactgctttc caaaaatcct
    38041 gagtctgagc cgtagaacgg aatccaaaca gaggtaaact tgcctgtatc aatactgtgt
    38101 tcttccatga agtagacacc aacgtagatg ccgatgtttt tagcacccag tgatgctagt
    38161 tttgctcgaa agttttcgac accttcgttc atattagaca tggttttgtc ttccacgtca
    38221 agccaatagt aactagggct gtaaggagag gcagcattgt agaaaacttc ggcagccttt
    38281 tccatttctt ggacactttt tccagctaca taagcgtaga cagcaactgg gacattccgc
    38341 ttttgaagtt cagtgatatg actcttatag gccttgtcta ttccattgat aaatgaagca
    38401 tcattttctt ttgtcgtttg agcaccactg tgaacacgaa caatagcacc tgaaatattt
    38461 tgtgagaggg catcgtagtt gatttcctca ggacgctgcc agccagagag gtcaataatc
    38521 ggtttgtcta agtgtttcaa agcctgtgct tcaatctgtg ctatattgga ttttgtttta
    38581 aacgattggc tgtcattaag tgggcgattg atgattaaaa tgaacatcat aatcccaaaa
    38641 aaactaaata aaataagtgg atgaatttgt tttctcatat cttataattc taccctaaaa
    38701 atcaaaaaaa atcaaaaaaa tgggttaagg aagagacttt agagcatttt ttcattcaag
    38761 agtgcggaat gatttgaaat atggtataat aaaagggaat ttctacagaa aagggaagat
    38821 tatgtcaaat tttgccatta ttttagcagc gggtaaaggg actcgcatga aatctgattt
    38881 gccaaaagtt ttgcacaagg ttgcgggtat ttctatgttg gaacatgttt tccgtagtgt
    38941 gggagctatc caacctgaaa agacagtaac agttgtagga cacaaggcag aattggttga
    39001 ggaggtcttg gctggacaga cagaatttgt gactcaatct gaacagttgg gaactggtca
    39061 tgcagttatg atgacagagc ctatcttaga aggtttgtca ggacacacct tggtcattgc
    39121 aggagatact cctttaatca ctggtgaaag cttgaaaaac ttgattgatt tccatatcaa
    39181 tcataaaaat gtggccacta tcttgactgc tgaaacggat aatccttttg gttatggacg
    39241 aattgttcgt aatgacaatg ctgaggttct tcgtattgtt gagcagaagg atgctacaga
    39301 ttttgaaaag caaatcaagg aaatcaacac tggaacatac gtctttgaca acgagcgttt
    39361 gtttgaggct ttgaaaaata tcaataccaa taacgctcaa ggcgaatact atattacaga
    39421 cgtcattggt attttccgtg aaactggtga aaaagttggc gcttatactt tgaaagattt
    39481 tgatgaaagt cttggggtaa atgaccgtgt ggcgcttgcg acagctgagt cagttatgcg
    39541 tcgtcgcatc aatcataaac acatggtcaa cggtgttagc tttgtcaatc cagaagcaac
    39601 ttatatcgat attgatgttg agattgcttc ggaagttcaa atcgaagcca atgttatctt
    39661 gaaagggcaa acgaaaattg gtgctgagac tgttttgaca aacggtactt atgtagtgga
    39721 cagcactatc ggagcaggag cggtcattac caattctatg attgaggaaa gtagtgttgc
    39781 agacggtgtg atagtcggtc cttatgctca cattcgtcca aattcaagtc tgggtgccca
    39841 agttcatatt ggtaactttg ttgaggtgaa aggatcttca atcggtgaga ataccaaggc
    39901 tggtcatttg acttatatcg gaaactgtga agtgggaagc aacgttaatt tcggtgctgg
    39961 aactattaca gtcaactatg acggcaaaaa caaatacaag acagtcattg gaaacaatgt
    40021 ctttgttggt tcaaattcaa ccattattgc accagtagaa cttggtgaca attccctcgt
    40081 tggtgctggt tcaactatta ctaaagacgt gccagcagat gctattgcta ttggtcgcgg
    40141 tcgtcagatc aataaagacg aatatgcaac acgtcttcct catcatccta agaaccagta
    40201 ggagcctatc atggagtttg aagaaaaaac gcttagccga aaagaaatct atcaaggacc
    40261 aatatttaaa ctggtccaag atcaggttga attaccagaa ggcaagggaa ctgcccaacg
    40321 ggatttgatt ttccacaatg gggctgtctg tgttttagca gtaacggatg aacaaaaact
    40381 tatcttggtc aagcagtacc gcaaagctat cgaggctgtc tcttacgaaa ttccagccgg
    40441 aaaattggaa gtaggagaaa acacagcccc tgtggcagct gcccttcgtg aattagagga
    40501 agaaacagcc tatacaggga aattagaact cttgtacgat ttttattcag ctattggctt
    40561 ttgtaatgag aagttaaaac tatatttagc aagcgatttg acaaaagtgg aaaatccgcg
    40621 tccgcaggat gaggatgaaa ccttggaagt ccttgaagtg agcttagaag aagcgaaaga
    40681 attaatccaa tcaggtcata tttgtgatgc caagacaatt atggctgttc agtattggga
    40741 gttgcagaaa aaatagagga ggtcagtatg ggtaaatctt tattaacgga tgaaatgatt
    40801 gaaagagcta atagaggcga aaaaatttca ggtcctcctt tgctagatga taatgaggaa
    40861 actaagattt taccaacctc ttcttcccgt tttggttatg ccaatcctaa ggatcatggt
    40921 tttagccagg aaaccttgaa gattcaggtc gaaccatcta ttcataaaag ccgtcgtatt
    40981 gaaaatacca agagaaatgt cttcaattct aagttgaata aaatcttatt tgcggtcatc
    41041 tttctcttga ttttgcttgt tttagcaatg aaacttttgt aatagaaaag gaattgaaat
    41101 gaaaatagga attattgctg ctatgccaga agaactggct tatctggtcc agcatttaga
    41161 taatgcccag gagcaagttg ttttggggaa tacctatcat acaggaacca ttgcttctca
    41221 tgaagtcgtt cttgtagaaa gtggaattgg taaggtcatg tctgctatga gtgtggcgat
    41281 tttggctgat catttccagg tggatgccct tattaatacg ggttcagctg gggcagtagc
    41341 agaaggtatc gctgttgggg atgtcgtgat tgctgacaaa ttagcctatc atgacgtgga
    41401 tgtcacagct tttggctatg cttatggaca aatggcgcaa caaccgcttt atttcgaatc
    41461 agacaaaacc tttgttgctc aaatccaaga gagtttatct caattggacc aaaactggca
    41521 tcttggtttg attgctacag gagatagttt tgttgcagga aatgacaaga tagaagcgat
    41581 taagtcccat ttcccagaag ttttagccgt ggagatggag ggggcagcta ttgctcaagc
    41641 agcgcatgcc ctcaatctcc cagtcttagt catccgagct atgagtgaca atgccaacca
    41701 tgaagcaaac atcttttttg atgagtttat tatcgaagct ggacgtcgct ctgcccaagt
    41761 cttgttgacc tttttgaagg ctttagatta agcggaaatt tgacagtttt tctagcttat
    41821 gataagattt aagtaaagaa aagctagaaa acgtttcaga ggatattatg agtattgaaa
    41881 tgaccgtcag tgagattgca gaggtcttag gattatctcg ccaagcaatc aataaccgtg
    41941 tcaaagaatt accagaagaa gacacagata aaaatgacaa aggggtaaca gttgttacca
    42001 gaagtggctt gattaagcta gaagaaatct ataaaaaaac gatttttgaa gatgagcctg
    42061 tcagtgaaga tgtcaaacaa cgtgaactga tggagattct agtggatgag aagaatgcag
    42121 aaattcttcg tctttatgaa caattaaaag ccaaggatcg tcagttatca gaaaaagacg
    42181 agcagatgcg tatcaaagac cgtcagattg ctgagaaaga caaacaatta gatcagcaac
    42241 aacaattgac ccttcaagcc atgaaggatc aagaaaatct taagctagag ctggaccaag
    42301 caaaagaaga agtccaatcc actaagaaag gcttttttgc tcgtttattt ggaggataat
    42361 caaggagctg tttaggttaa atgctaacct gatggcttct tttgtttgtg aatagtatag
    42421 ttgattagaa cagagacagt agaggagagg tacagaatgg aacgattaga agattacatt
    42481 gcttttgact tagaatttaa tcagcacgaa ggacaaacac acttgattca agtatcggca
    42541 gtgcgtttta gagatggtcg ggaaatcgat gcctatgatt cctatgttca taccagcgtc
    42601 cctctaaaaa gctttatcaa tggtttgacc gggattacag ctgagacctt aaaagatgct
    42661 cctccagttg agaaagtctt agaagaattc caagagtttg tgggttcttt gctgatgata
    42721 ggctacaatg ctgctaaaag tgatttacct attttagcag agcatggttt agactatagc
    42781 caacagtaca aggtggattt gtatgatgaa gcttttgaac gtcggagttc agatttacat
    42841 ggtattgcca atctcaaatt gcaaactgtt gcctcatttc ttggatttca aggtcagtct
    42901 cataatagtt tagaggatgc tcggatgacg gcgcgtgttt acgaatcctt cttagagtcg
    42961 gatcaagcta gggaattgtt agagacagaa agtagcttat caaataatcc ttttggtggc
    43021 ttggacttgt ctcaattatt tgactaggag tgcctatgaa acctgaaaaa cctaaaaaat
    43081 tgggtcttaa acagatttac ctcaatcttg ataaaatcct ctttctattt ttcttgattt
    43141 ttatgatagt ggagtatttg tgggtaccat tcaattcatg gttggctagc ttcttattag
    43201 atcagacagg ttatcttttt atttcctata acaatgtctt agccatcctt actcattctc
    43261 cattgattag tctagctttt ttagccttga ttgtaattaa ctttctggtt gcttattttc
    43321 aaataggact cctctttatt ggtgctcgtc atctcctcta taatgaaaag cgaaggctaa
    43381 ttgagtatat ccgcaaggtt ttccatgaaa gttttgaatt tatgaaaaag ctaactggcg
    43441 ctaaagcctt gtttatcttt ttctatatag ctatgctgtt tccttttata cggaaaatgc
    43501 tgaagattta ttatcttaat aaaatcgtca ttccagagtt tatccaagct tatttagaag
    43561 ataaatattg gatgtggtgg ctgagcatcc tacttctatc tcttatcttt ctctatgtct
    43621 ctgtcagatt gatgtttgcc cttccgaaga ttgtatatga tcagctgact gttcgagaag
    43681 cagtgatgtt tagcttggag aaaaccaaga agagagttac tttttatgct tggaatttat
    43741 tttacatctt gctcaaagca catttattat tttatcttcc tttgattcct ttattattgg
    43801 ctcaaacttt ggtagatgat atcactcaga gagagtctct gattcttggt attttgaatt
    43861 ttgttgtgat taaaaatctc tattatatgg ctctgactta ttttttggtt aaatttgttt
    43921 catttttgac aggaaaggag ctggatatgc ttcctaggag agaaaaagat cacatcgtgc
    43981 gatggggtgt catgacttgt gccagtcttt tctttgcctt agaaggttat atttatctgg
    44041 aggctcccat ggttcatcta cctcaactta tttctcaccg aggggtttcc aatgcaaatg
    44101 ggattcaaaa tacagtagag tccttggaaa ctacagcaca actcaaacca gacttggtgg
    44161 agacggacgt gcaggaaaca aaagatgggc agtttgtcat gatgcatgat gctaacttga
    44221 aaaatctagc aggtatcaat aaaagtcctc aagacttaaa cttggaggag cttaaaggga
    44281 ttgatatttt tgaaaatggc taccagacta aaatttcaag ctttgaagat tatctcagtc
    44341 gagccaacga acttggtcaa aaattactaa ttgaaattaa aaccagtaaa aaagatagtc
    44401 cagacatgat gaaccgcttt ttagcccgtt atgctgcaaa gctcaagatt tatggacatc
    44461 aaatccagtc tttagactac catgttgtcg aaaaagtaag acagtatgat gccgaactgc
    44521 cagtttattt catcatgccc tacaattctg tctttcctaa aacaagagcg acaggatata
    44581 ctatggagta ctcaaccttg gatgaatatt ttgtaagcaa actatggaca acggatcaga
    44641 aactttatgt ctggactgtc aatgattcag aagctatcag caaatctctt cacttaggag
    44701 tagatggagt gattacagat gatttggaaa aagttcagcg ggaaatagaa gtagcccaag
    44761 aagatccaga atatacggat ttgctcttga aaaaagcatt ggaattcttt gaattttagt
    44821 aataaaaaag aggaacggtt gttcctccca tgataggtaa gtattc
//
LOCUS       DBERDK010000009        42530 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.009, whole genome shotgun sequence.
ACCESSION   DBERDK010000009 DBERDK010000000
VERSION     DBERDK010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 42530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42530
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.009"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(294..2021)
                     /gene="ezrA"
                     /locus_tag="ACL73L_000462"
     CDS             complement(294..2021)
                     /gene="ezrA"
                     /locus_tag="ACL73L_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HGS0771498.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEERKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAEGYNHSF
                     RFLKASHQIDQIESQITLIEEDIAAIRNALADLEKQESKNSGRVLHALDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLESGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASHVRRIQTELESFEAAIVEVTSNQEEPTQAYSVLEENLEDL
                     QTQLKDIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRVLEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVAEPGVTNRFVTSY
                     EKTRETIRF"
     gene            complement(2103..4049)
                     /gene="gyrB"
                     /locus_tag="ACL73L_000463"
     CDS             complement(2103..4049)
                     /gene="gyrB"
                     /locus_tag="ACL73L_000463"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HGS0771499.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAD
                     LEVVGDTDRTGTTVHFIPDPKIFTETTTFDFDKLNKRIQELAFLNRGLQISITDKRQG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTGEDVREGLTAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            complement(4064..4627)
                     /locus_tag="ACL73L_000464"
     CDS             complement(4064..4627)
                     /locus_tag="ACL73L_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HGS0771500.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQT
                     LKVSTPFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEDILNQGGRHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            complement(4712..5266)
                     /locus_tag="ACL73L_000465"
     CDS             complement(4712..5266)
                     /locus_tag="ACL73L_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1 family glyoxalase III"
                     /protein_id="HGS0771501.1"
                     /translation="MVKVAVMLAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGDLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLAAICAA
                     PIALNQAEILKNKRYTCYDGVQEQILDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYRDVFGKNQ"
     gene            complement(5277..6500)
                     /locus_tag="ACL73L_000466"
     CDS             complement(5277..6500)
                     /locus_tag="ACL73L_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HGS0771502.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPSLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLARVIVQFTKKHKEWRRTVSLDFLLIFWMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKSG
                     KVPFKRKKVVLKQIK"
     gene            complement(6537..8987)
                     /locus_tag="ACL73L_000467"
     CDS             complement(6537..8987)
                     /locus_tag="ACL73L_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HGS0771503.1"
                     /translation="MKTIGNRYVVVDLEATSTGSKAKIIQVGIVVIEDGEIVDHYTTD
                     VNPHEPLDVHIKELTGLTDQRLAQAPDFSQVARKIFDLVEDGIFVAHNVQFDANLLAE
                     NLFFEGYELRNPRVDTVELAQVFFPELEKYSLPILCRELGIPLKHAHTALSDAQATAE
                     LLLFLREKMTQLPKGLLERLLEMADALLYESYLVIEETYRNQSILSSPDLVQVQGLYF
                     KKTAASLEPRKLSQDFSKNISLLNLEVREEQESFAKEVGLLLKDEPVSLIQAPTGIGK
                     TYGYLLPALSQSKERQIVLSVPTKILQNQIMEEEGKRLKEVFHTDIHSLKGPQNYLKL
                     DAFYHSLQENDENRLFRRFKMQVLVWLTETETGDLDEIGQLYRYQHFLADLRHDGNLS
                     SQSLFVTEDFWKRSQERAETCKLLVTNHAYLVTRLEDNPEFVSDRLLIIDEVQKILLA
                     LENLLQETYDIQSIIDLIDKALVGEENRVQQRILESIRFECLYLIEQFQSGKSRKNIL
                     DSLDNLHQYFSELEVEGFDELVRYFTAEGDYWLEVTETSQKKIQISSTKSCRTLLSSL
                     LPESCQVLGVSATLEISQRVSLADLLGYPEAKFVKIESRGKQEQEVVMVKDFPLVTET
                     SLEVYAREVAALLVEIQAFQQPILVLFTAKDMLLAVSDLLTVSHLAQYKNGDVHQLKK
                     RFEKGEQQILLGAASFWEGVDFSSHPFVIQVVPRLPFQNPQEPLTKKINQELNQEGKN
                     AFYDYQLPMAIIRLKQALGRSMRREYQRSLTLILDRRIIGKRYGKQIVASLAEEATIK
                     TISRSEVDEAIDRFFNEL"
     gene            complement(9187..10116)
                     /locus_tag="ACL73L_000468"
     CDS             complement(9187..10116)
                     /locus_tag="ACL73L_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HGS0771504.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELLVAVNALMHQDMMDRIKPFLDFLEEIKTDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARNWIQEGNFSH
                     DQAFLLDEEVRKLHPKNRFLDTGFYDYDPDMVR"
     gene            10256..10543
                     /locus_tag="ACL73L_000469"
     CDS             10256..10543
                     /locus_tag="ACL73L_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HGS0771505.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     NIAVFCICIAIFSFIFLALKLSTALAFAAAIGFSLLVLKVQRSIIKRKRRR"
     gene            complement(10577..11911)
                     /gene="ciaH"
                     /locus_tag="ACL73L_000470"
     CDS             complement(10577..11911)
                     /gene="ciaH"
                     /locus_tag="ACL73L_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HGS0771506.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(11901..12575)
                     /gene="ciaR"
                     /locus_tag="ACL73L_000471"
     CDS             complement(11901..12575)
                     /gene="ciaR"
                     /locus_tag="ACL73L_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HGS0771507.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
     gene            complement(12684..15230)
                     /locus_tag="ACL73L_000472"
     CDS             complement(12684..15230)
                     /locus_tag="ACL73L_000472"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HGS0771508.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGQAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLSLRFDQAEGELALSNMP
                     EIDVENRKETGIWKFETTPRMSSYLLAFVAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVRSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFAKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENDVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEIPGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLEAVLAELETLDNTSKLQIVQERRLLAEAGHISYADL
                     LPVLDKLAKEESYLVVSAVSQVISALERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEASQVASQIFATHKENLAGLPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLAAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQRLA
                     EYKEFFEPQLSDLALSRNIGMGIKEIAARVDLISREKAAVEAVVLQYGNA"
     gene            complement(15353..15793)
                     /locus_tag="ACL73L_000473"
     CDS             complement(15353..15793)
                     /locus_tag="ACL73L_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HGS0771509.1"
                     /translation="MIPQEMWNKYKQINPLIGDEIDAWAFGVEPDLLADLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDVILDSQNQSVCIVEITKVSVELFNQVSAQHAFKEGE
                     GDKSLAYWRQAHEDFFTDCLGEVGLTFTPESKVVLEEFRKVYPL"
     gene            complement(15768..16637)
                     /locus_tag="ACL73L_000474"
     CDS             complement(15768..16637)
                     /locus_tag="ACL73L_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HGS0771510.1"
                     /translation="MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNIILAS
                     CQQFVKDYLDTICSFYLGKEVWYRFSELTNTEANCLVGTREFFDEGHPLFGYRGTRRL
                     LACLDEFQAEAHVVTEVYQTNPNLSVIFPSVNDADQLKQAITVLRQYGFTGKVGTMIE
                     LPSAYFDLSSILETGISKIVVGMNDLTSFVFATMRNSQWHDMESPIMLDMLRDMQDKA
                     RKNKINFAVAGYLNTSFIQKMNQLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKK
                     LQRSTHDTARNVE"
     gene            complement(16679..17407)
                     /locus_tag="ACL73L_000475"
     CDS             complement(16679..17407)
                     /locus_tag="ACL73L_000475"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS0771511.1"
                     /translation="MELFKTWKKNMVLYGLKSQIGTVYRNNDRTTSFYDVGNFLYLAG
                     ELDSRFWEDFVRKYGLDYKIIISENTNWQDFLHRKVGLNSFTRYSFKDKANFQVEFLN
                     NLVTHLEEGYNIVPIDNHIYNCFSTEEWSQDLQGDFESYQDFVLKGGFGFVILKNNEL
                     IAGISSGLVYRKAVEVEVATRPNEQGNGLAKKLGAAMILESLNRDMFPLWDAHNEASK
                     KVAEFLGYELSEPYEAFELEEILI"
     gene            complement(17415..17894)
                     /locus_tag="ACL73L_000476"
     CDS             complement(17415..17894)
                     /locus_tag="ACL73L_000476"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HGS0771512.1"
                     /translation="MEIKKHFGVYAVCFENGKLLCIEKTRGPYQHRYDLPGGSQQLGE
                     GLTETLTREVMEETGFTVRSYSNPRIYDVFVREELTNFMVHHVMALYDVEMNESAPQV
                     TTSEAVSDGANDSLGYIWMDIQEITEENASPLVLKVKSELLGFPELDKTSYMNWKVK"
     gene            complement(17905..18603)
                     /locus_tag="ACL73L_000477"
     CDS             complement(17905..18603)
                     /locus_tag="ACL73L_000477"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HGS0771513.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGYQVYGVDQGEKPLLEGDF
                     HFLQRDLTLDLEPIFDWCPQVDVLCNTAGVLDDYKPLLEQTAQDIQEIFEINYIIPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEAGI
                     QVFGIAPGAVKTAMTAADFEPGGLADWVASETPIKRWIEPEEIAELSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            complement(18596..18832)
                     /locus_tag="ACL73L_000478"
     CDS             complement(18596..18832)
                     /locus_tag="ACL73L_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018367459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HGS0771514.1"
                     /translation="MTFEEILPGLKAKRKYVRTGWGGAENYVQLFDTIEQNGLALEMT
                     PYFLINVSGEGEGFSMWSPTVCDVLATDWVEVHD"
     gene            complement(18928..19854)
                     /locus_tag="ACL73L_000479"
     CDS             complement(18928..19854)
                     /locus_tag="ACL73L_000479"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HGS0771515.1"
                     /translation="MRYITLGQDDKELSEIVLGMMRIKDKSVKEVEEFVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNRPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDALMESDQVAEAFDLLYKQGKVRDFGVSNQNPMMMELL
                     KKDVKQPLAVNQLQLSVAFTPGFESAFHVNMEDSQAAMRDGSIFEYCKLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSTIAISWILRYPAKMQAVVGTTN
                     PKHLREVSQAANFSLTRKEWYEIYLAAGNNLP"
     gene            complement(19929..20744)
                     /locus_tag="ACL73L_000480"
     CDS             complement(19929..20744)
                     /locus_tag="ACL73L_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771516.1"
                     /translation="MKKMKYYEETSALLHEFSEENQKYFEELWESFNLAGFLYDEDYL
                     REQIYLMMLDFSEAERDGMSAEDYLGKNPKKIMKEILKGAPRSSIKESLLTPILVLAV
                     LRYYQLLSDFSKGPLLTVNLLTFLGQLLIFLIGFGLVATILRRSLVQDSPKMKFGTYI
                     VVGTIVLLVVLGYVGMASFIQEGAFYIPDPWDSLSVFTISLVIGIWNWKEAVFRPFVS
                     MIIAHLVVGSLLRYYEWMGISNVFLTKVIPLAVLFIGIFLLFHGFKKIKWSEV"
     gene            complement(20748..21068)
                     /locus_tag="ACL73L_000481"
     CDS             complement(20748..21068)
                     /locus_tag="ACL73L_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HGS0771517.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMRPSPDGPDRKYFSLMKEGEERVSVFWQQWDDLSQKVEGI
                     KNGG"
     gene            complement(21158..21394)
                     /locus_tag="ACL73L_000482"
     CDS             complement(21158..21394)
                     /locus_tag="ACL73L_000482"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771518.1"
                     /translation="MKIKEQTRKLAAGCSKHCFEVVDRTDEVSSKHVFEVVDETDEVS
                     SKHVFEVVDETDEVSNHTYGRATLTWFEEIFEEY"
     gene            complement(21444..23441)
                     /gene="metG"
                     /locus_tag="ACL73L_000483"
     CDS             complement(21444..23441)
                     /gene="metG"
                     /locus_tag="ACL73L_000483"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HGS0771519.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLAKDEAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIVAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTLLTVDPAVPNGSVIG"
     gene            complement(23565..24824)
                     /locus_tag="ACL73L_000484"
     CDS             complement(23565..24824)
                     /locus_tag="ACL73L_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS0771520.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKSQKNISVFFSPKKSKDGSFTQKQSAFTVSGKEEEVPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENANLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKRSKIYGFGGLP
                     ITTNISLAANFNVDEIASIVFRVNDTSLTPTLGPELARKMTELAGLQQGEYQVADESL
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSIPVALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
     gene            complement(24826..25527)
                     /locus_tag="ACL73L_000485"
     CDS             complement(24826..25527)
                     /locus_tag="ACL73L_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0771521.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELTERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAAYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            complement(25511..26710)
                     /locus_tag="ACL73L_000486"
     CDS             complement(25511..26710)
                     /locus_tag="ACL73L_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HGS0771522.1"
                     /translation="MKKKNGKAKKWQLYAAIGAASVVVLGAGGILLFRQPSQTALKDE
                     PTHLVVAKEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKVSEGQALVK
                     YSSSEAQAAYDSASRAVAKADRHINELNQARNEAASAQAPQLPAPVGGEDATVQSPTP
                     VAGNSVASIDAQLGDARDARADAAAQLSKAQSQLDATTVLSTLEGTVVEVNSNVSKSP
                     TGASQVMVHIVSNENLQVKGELSEYNLANLSVGQEVSFTSKVYPDKKWTGKLSYISDY
                     PKNNGEAASPAAGNNTGSKYPYTIDVTGEVGDLKQGFSVNIEVKSKTKAILVPVSSLV
                     MDDSKNYVWIVDEQQKAKKVEVSLGNADAENQEITSGLTNGAKVISNPTSSLEEGKEV
                     KADEATN"
     gene            26996..28342
                     /gene="gor"
                     /locus_tag="ACL73L_000487"
     CDS             26996..28342
                     /gene="gor"
                     /locus_tag="ACL73L_000487"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HGS0771523.1"
                     /translation="MREYDIIAIGGGSGGIATMNRAGEHGAKAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQIAETFHQFGEDYGFKTTDLNFDFATLRRNREAYIDRARSSYDGSF
                     KRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVF
                     AWEELPESVAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRGFDSYIVEGLVKEME
                     RTNLPLHTHKVPVKLEKTTDGITIHFEDGTSHTASQVIWATGRRPNVKGLQLEKAGVT
                     LNERGFIQVDEYQNTVVEGLYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTAKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFASMYSSCTRNRQESRFKLI
                     TAGSEEKVVGLHGIGYGVDEMIQGFAVAIKMGATKADFDATVAIHPTASEEFVTMR"
     gene            28539..29075
                     /locus_tag="ACL73L_000488"
     CDS             28539..29075
                     /locus_tag="ACL73L_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HGS0771524.1"
                     /translation="MKKAHVYAIPAIGAALIAVLAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAVLSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLHFLAGMAFEKALVVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQAYFTN"
     gene            29136..29282
                     /locus_tag="ACL73L_000489"
     CDS             29136..29282
                     /locus_tag="ACL73L_000489"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771525.1"
                     /translation="MKIKEQTRKLAAGCSKHCFEVVDETDEVSNHTYGKAKLMRFEEI
                     FEEY"
     gene            complement(29279..29494)
                     /locus_tag="ACL73L_000490"
     CDS             complement(29279..29494)
                     /locus_tag="ACL73L_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HGS0771526.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGASAKFDIRAWS
                     PDHTKMGKGITLSNEEFQTMVDAFKGN"
     gene            29592..29912
                     /locus_tag="ACL73L_000491"
     CDS             29592..29912
                     /locus_tag="ACL73L_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771527.1"
                     /translation="MRHKFQQVLNKIHDFLNGYDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFSKNVSRIIRISDIQRLRFVPSTVQTAQKNR
                     FKKE"
     gene            complement(29943..30281)
                     /locus_tag="ACL73L_000492"
     CDS             complement(29943..30281)
                     /locus_tag="ACL73L_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HGS0771528.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVERATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFEQIR"
     gene            complement(30293..31012)
                     /gene="trmD"
                     /locus_tag="ACL73L_000493"
     CDS             complement(30293..31012)
                     /gene="trmD"
                     /locus_tag="ACL73L_000493"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HGS0771529.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDEPYGGGQGMLLRAQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            complement(31002..31520)
                     /gene="rimM"
                     /locus_tag="ACL73L_000494"
     CDS             complement(31002..31520)
                     /gene="rimM"
                     /locus_tag="ACL73L_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HGS0771530.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELALFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKGYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPNKRV
                     DVEILEGLDDED"
     gene            complement(31590..32338)
                     /locus_tag="ACL73L_000495"
                     /pseudo
     CDS             complement(31590..32338)
                     /locus_tag="ACL73L_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819199.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
     gene            complement(32442..32766)
                     /locus_tag="ACL73L_000496"
                     /pseudo
     CDS             complement(32442..32766)
                     /locus_tag="ACL73L_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654120.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
     gene            complement(32915..33154)
                     /gene="kphA"
                     /locus_tag="ACL73L_000497"
     CDS             complement(32915..33154)
                     /gene="kphA"
                     /locus_tag="ACL73L_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein KphA"
                     /protein_id="HGS0771531.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
     gene            complement(33174..33446)
                     /gene="rpsP"
                     /locus_tag="ACL73L_000498"
     CDS             complement(33174..33446)
                     /gene="rpsP"
                     /locus_tag="ACL73L_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HGS0771532.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLAWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
     gene            33616..33825
                     /locus_tag="ACL73L_000499"
     CDS             33616..33825
                     /locus_tag="ACL73L_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771533.1"
                     /translation="MLSVNTILEKFYKEHQVKPFISPERELDTWLLSPKPVPKRNMDL
                     LADDSLAGDIILLWRIQFGTFTTET"
     gene            34304..34525
                     /locus_tag="ACL73L_000500"
     CDS             34304..34525
                     /locus_tag="ACL73L_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771534.1"
                     /translation="MSFYGLFYNGIAITPNTYLSAWFVNFIAALPLNFLIVEPIARFI
                     LSSFQKPFTGEEVEEVEDFQDDDEIPTII"
     gene            34609..35412
                     /locus_tag="ACL73L_000501"
     CDS             34609..35412
                     /locus_tag="ACL73L_000501"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HGS0771535.1"
                     /translation="MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNDALTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQPNTNGSQFFINQNSTDTSSKLPTSKYPQKIIEAYKEGGNPSLD
                     GKHPVFGQVIGGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS"
     gene            complement(35469..37010)
                     /locus_tag="ACL73L_000502"
     CDS             complement(35469..37010)
                     /locus_tag="ACL73L_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HGS0771536.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMKMAEDGADVDALMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQQLTRYSGDYYQFQEVYAMKKSQLEAAYERQQKEIADLKDFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQAKNLQIGY
                     DRPLTKPLNLTFERNQKVAIIGANGIGKTTLLKSLLGIISPIAGEVERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKEYRGSILMVCHELDFYEGWIDQIWD
                     FNNLT"
     gene            complement(37012..37494)
                     /locus_tag="ACL73L_000503"
     CDS             complement(37012..37494)
                     /locus_tag="ACL73L_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HGS0771537.1"
                     /translation="MMKKTYNHILVWGVIFYSICIVCFCFTPQEQSTVGVGTPGIQHL
                     GRLVFLLTPFNSLWKLGEVSDIGQLCWIFLQNILNVFLFFPLIFQLLYLFPNLRKTKK
                     VLLFSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLAWLLYKRLHKNKIRN
                     "
     gene            complement(37494..38579)
                     /gene="rlmN"
                     /locus_tag="ACL73L_000504"
     CDS             complement(37494..38579)
                     /gene="rlmN"
                     /locus_tag="ACL73L_000504"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HGS0771538.1"
                     /translation="MKPSIHSLVHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEE
                     MTNLSKDLIAKLNDQFVVNPLKQRIVQESADGTVKYLFELPDGMLIETVLMRQHYGLS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFDERGQDERISHIVVM
                     GIGEPFDNYNNVLNFFRTINDDKGMAIGARHITVSTSGLAHKIRDFADEGVQVNLAVS
                     LHAPNNELRSSIMKINRAFPIEKLFAAIEYYIETTNRGVTFEYIMLNEVNDGVEQALE
                     LTELLKNIKKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDTLKKKGVNCVVRQEHGT
                     DIDAACGQLRSNTMKRDRQKAVAAVNP"
     gene            complement(38600..39130)
                     /locus_tag="ACL73L_000505"
     CDS             complement(38600..39130)
                     /locus_tag="ACL73L_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HGS0771539.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFSLVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISRLQDYLLEYCS
                     YGCAYFVLENEAPKRASFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDQH
                     SQKEDKGQRHFVIRQK"
     gene            complement(39286..39891)
                     /gene="sodA"
                     /locus_tag="ACL73L_000506"
     CDS             complement(39286..39891)
                     /gene="sodA"
                     /locus_tag="ACL73L_000506"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase SodA"
                     /protein_id="HGS0771540.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADVESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELAAA
                     IDATFGSFEEFQAAFTAAATTRFGSGWAWLVVNKEGKLEVTSTANQDTPISEGKKPIL
                     GLDVWEHAYYVKYRNVRPDYIKAFFSVINWNKVDELYAAAK"
     gene            complement(40064..41101)
                     /gene="holA"
                     /locus_tag="ACL73L_000507"
     CDS             complement(40064..41101)
                     /gene="holA"
                     /locus_tag="ACL73L_000507"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HGS0771541.1"
                     /translation="MLAIEESQKLTLSNLPSLSLFTGTDQGQFEVMKSQMLKQIGYDS
                     ADLNFAYFDMKEVVYKDVELELVSLPFFADEKIVILDYFMDITTAKKRFLTDDELKSF
                     EEYLDNPSPTTKLIIFAEGKLDSKRRLVKLLKRDAKVFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNHSFENLLIKSGFQFSEIQKNLLFLQSYKADSVIEEEDIVNAIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            complement(41135..42070)
                     /locus_tag="ACL73L_000508"
     CDS             complement(41135..42070)
                     /locus_tag="ACL73L_000508"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HGS0771542.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIEELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQDVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFAYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDRITNELKAIMVEKGYESLEDFRGKLRYID"
BASE COUNT        12659 a         9270 c         7623 g        12978 t
ORIGIN      
        1 aagttactca aagtacagat ttgaagctgc agataagact gacgaaatca ctttaatact
       61 aaatgaaaat caaaaagcaa actaagaagc tagcctcagg ctgtacttga gtacggtaag
      121 gcgacgctga cgtgatttga agagattttc gaagagtata aaacactgtt ttgaggtggt
      181 ggataaatcg gctgggtaaa aactcaatct caggagaaaa tgaagtaaat ctccccacaa
      241 ctatagaaaa agatttgatt ctgctcctca cacaataaaa tctttttctt ttattaaaaa
      301 cgaatcgttt cacgtgtttt ctcatatgag gtaacaaagc gattggttac accaggctct
      361 gccacttcca atgcttgaga aatcttgtca aatgaagcgt gataatcaaa ttctttttca
      421 aaaatatcta aagcttcgtt aaatgcttct tgaatgcgtt catcaaatga gcgatagcgg
      481 ttagaatatt gcaagagttg ctctgtcaaa gttgcatatt gtacaatatt ataagtttcc
      541 gtttctaaag cttccatatc attcgttgca atttcaagaa ctcgggtaac agattcaatg
      601 ttaatcattt tttgttctaa ctcaaccatt aaatcctcgg tattattgct tgccgtaaag
      661 aataacttca agaaagtttg tggaatacct ggcagattgc gtttttccat gtatcgcttg
      721 atagtatgga gacgattgac ataaacattg gccttttgac gtgcattaat atcatctttc
      781 tcaatttgtg tcaggcgctc actaactgaa atttgctcat cttcaatatc ttttagttga
      841 gtttgtaaat cctcaagatt ttcttcaaga actgaataag cttgggttgg ttcttcttga
      901 tttgaagtta cctcaacaat agctgcctca aaactctcta attctgtctg aatacgacga
      961 acatggcttg cagctgtctc aggaagtaaa taggtcttgt tcaaacgtgc aatatcttct
     1021 cccaataaag tattattctc tttcatatgt tgaaggtaag ttggaagagt tgcaagtaga
     1081 ttttccacta ctttctgagc agcaatttct cgagtaaaaa tatcatacaa ggcattgatt
     1141 tcctcttgtg cctgtccatt ctcatattcg gcattatcca attccaactg acgaatattc
     1201 tcttggtttt tcttgaatgc ttcataaagc aagtggaaac gcgcttcaat atccgtttca
     1261 acaaaatgat aattagcatc aattagttta cgataaccgg attccaaatc ctgtaattga
     1321 tctggcaatt ctgtagaaag cgtcgtaacc aaggctggaa cacgatccac aatatgactt
     1381 aaggccaaaa tgtgattttc tgtattatcc aaaatcactg cggcttccac agggtcaccc
     1441 gatgaattca aggttacaaa ttgtgaaaat tcagattgga tattttctaa ttgtttttca
     1501 atttcatcca aggcttgacc atactgttct gaattttcag caactctatg ctgaagttcc
     1561 tcaaataaat ccaaagcatg aagaacacga ccactatttt tagattcttg cttctctaag
     1621 tctgccaaag cattgcgaat tgccgcaata tcttcttcaa tcaaagtaat ttgactctca
     1681 atttggtcaa tttgatgact ggccttgaga aaacgaaatg aatggttata accttctgct
     1741 tcaaagagat tattttcaat atcggcaaaa gagttgagag ataaatcgac ccatttttga
     1801 ttccattcac ggaaagccac ttgactttgt ccaatcaagt gcatattttt tacagcttct
     1861 acttcatcat ttactggaag attgtatagt tcttcttttc tttcttctag cgcctctaat
     1921 ctcccctcgt ttcgcttacg tagaaagatt gccactacat aagccagaac taaaatgact
     1981 gcaattgcaa ccattaaata aattagttgt ccattagaca tatcaaactc cttttttact
     2041 tgaaacaatc gtaacgatta tatcatattt tttagaccaa gggaactact tctccctatt
     2101 ttttagacat caagtgtact atagacagca ttttcttcga taaactcacg acgaggctct
     2161 actcgatccc ccatcaacat atcaaagatt ttatctgctt ctgcagcatc atctacagaa
     2221 actctagcca tcaagcgatg ttcgggatcc atggttgttt cccacagctg atgatcgtcc
     2281 atttcaccta gccccttata acgctgaata gtcggtttgg tacgaccttc actataacgg
     2341 gctaaagctt cttggagttt gatttcttga tctgcacccg gctggatata ttctttaatc
     2401 tcgctcccaa ccttgacacc atagattggt ggttgggcaa tataaacata accagcttct
     2461 aggattggtt tcatataacg ataaatcaag gttaaaagaa gggtacgaat atgggctcca
     2521 tcgacatcgg catcggtcat caaaacgagt ttttggtaac gggctttcga aacatcaaat
     2581 tctgcgccaa atcctgttcc catggctgtg aaaagactac gaatttcttc gttagctaga
     2641 atcttatcca tacttgcttt ttcaacgttc aaaatcttac cgcgaattgg aaggatagcc
     2701 tgaaactcac ggttacgacc agatttggct gatccaccag ctgagtctcc ttcgacgatg
     2761 aagagttctg tttcagcagg gttattagaa gaacagtctg ctagtttccc tggaaggttg
     2821 gaaatttcca aaccagattt tttacgtgtg acttcacgcg cacgcttggc agccacacga
     2881 gccttggcag ctaaaatccc tttttccacg atacgtttgg caatctgtgg attttccatg
     2941 aggaaatcgg agaaggcttc actgaagagg cgattggtaa tcttgaccac ttcgctattt
     3001 cccaatttgg tcttggtttg tccttcaaac tgtggatttg ggtgtttaac tgagataact
     3061 gcagttaatc cttcgcgaac atcttcccct gttaaattat cttcattgtc tttcagtaac
     3121 ttattcttac gagcataatc attgataaca cgagtcagtg ccgtacggaa accttgttca
     3181 tgcgttccac cttcatgggt atgaatatta ttggcgaaac tcatgacatt ttcatggtaa
     3241 cctgttgtgt actgcatggc tacctcaact gtaatatcat ccatctcacc gtctgtatag
     3301 attggtgtat caaagattac atccttgttc tcgttgatat attcaacgta actagcaatc
     3361 ccaccttcat aatgataatg cttggtttgt tccaaacctt ggcgcttatc agtgatagaa
     3421 atttgaagac cgcgatttag aaaggccaac tcttgaatcc gtttatttaa tttatcaaaa
     3481 tcaaaggttg ttgtttcagt aaagattttt gggtccggta taaagtgaac cgttgttcca
     3541 gttctatctg tatccccaac cacctcaagg tctgcaacaa catgaccacg acggtattct
     3601 tggtaatgaa tcttaccgtt tttatggaca tgaacgtcta attgagtgga aagggcatta
     3661 actactgacg accccacccc gtgaagacca cctgaaacct tgtatccacc accgccgaac
     3721 tttcctccag catgaaggac tgtaaagacg gtctcaacag caggacggcc tgttttttcc
     3781 tgaatatcga ctgggatacc acgcccatca tccacaacag taatcgaatc atctggctca
     3841 ataaaaactt gaatatggct ggcaaatcct gccaaggcct cgtcaattga gttatcaaca
     3901 atttcccaga ctagatggtg aagaccttct tttgaggttg atccaatgta catccctgga
     3961 cgcatacgaa cagcctctaa gccctctaaa acttgaattt gactggcatc ataatcctgt
     4021 gcctgcagat ttttgatttc ttctgtcatc ttattccttt ttcttatatg tctaatactt
     4081 gtcttaaatt cacgatactg gtaaacaagt gggtatcaag tcctgcagct tgacctgctt
     4141 cgatatcaat cggccgatca ccaatgacaa gaccagagct aatctgatac ttttctctta
     4201 aataaagcat ggattcggga tttggctttc tcttaaagcc tgagctagaa gtcaccactt
     4261 ctgtaaaata agctgctata gaggtttttt ctaaaatttc caaaacctga tcatttcgat
     4321 gagagaccaa aaaatgacgg ccaccttgat ttaaaatgtc ttccaatagg tcagaaactc
     4381 cttcaaataa aatcgggtgt tcaagctctc tggcttcatt ttccttgtac ttttctaaaa
     4441 aattctctaa attgggagcg aatgtctcaa tcgcaaaagg agtagaaacc tttaaagttt
     4501 gatagacact gtcatggtct tgtgtgatac catacagtgc caatgtttca acaaatgcag
     4561 ctgttgaagt ttcataatta tccagtaaag ttccacctaa atcccagatg taatcgtgat
     4621 atttcatacc cttcattata gcattttttt atgaaaaaag cgatgatttc cctgagtttt
     4681 ccacataaaa aacgagagaa taactcccgt tttactgatt tttaccaaag acatctcgat
     4741 agagcattcc tgttcgtaaa ctctctgcgt cccctcctag ttgctccacc aactcgtagg
     4801 caaaggcaag ggctgttgaa ggaccccgac tggttgtcaa ctgaccatct accactactg
     4861 tttccttgac gtagtgacca tcaaggattt gctcttgaac gccgtcataa caagtgtatc
     4921 gcttattttt caatatctct gcttgattga gggcaattgg tgccgcacaa atggctgcta
     4981 gtttcttccc ttcttgttcg aagctttgca attcttgaat caaggtctga ttatcacgta
     5041 aatgtgcaga accaggcata cctccaggaa gaacaatcat atcatagtct gataaatctc
     5101 catcaaagac atgatctgct cttacttgga ttgcatgcga acccgttact tgctcttcaa
     5161 aaccaaccat atcacatgtg atattggctc gacgcaagac atctacaact gtcaaggctt
     5221 caatttcttc aaagccctga gctaacataa ctgctacttt taccatgatt ttctccttat
     5281 ttaatttgtt ttaatacaac ctttttccgt ttgaatggga cttttccgct cttttcttca
     5341 gctagattag tctggtaact catcgataaa agcaaaccaa caccaatcag attactgata
     5401 atagctgatc ccccttgcga aatgaaaggc aagggaatcc ccgtcaaagg aagtagtcca
     5461 gtcacagcac cgatattctc aaagatgtgg aagagcaaca tcataatcaa acctgtggaa
     5521 atataagtgt agaactggtt atttgattta agagtaatct tcaacatacg gtaaatcaac
     5581 atgagataga gggcaataac caggacagag ccaataaagc caaaatcttc tgcaataacc
     5641 gtaaaaatca tatctgactc tcgaactggg ataagcagat tcgaagcatt aaatccctga
     5701 ccaaataagc caccactccc aatggcaatc tgcccttgag cctgctggta agtcgttgtt
     5761 tgggcaaact caaagggatt gagccaagcc aaaatccgat tgatttggta ggtcggcatt
     5821 ccaatctggt gaagaaaagc tcgtccgtcc ttgctaataa agatagctaa gaaaccagca
     5881 actcctgtta cagcagtcac aaatactggg ataataattt tccaagaaac ccctgataat
     5941 aaaacgattc ctgagaaaat ggctacaaaa accaaagccg tccccaagtc actttgaagt
     6001 gctaaaagaa ctaggactgg aatggtaaag agaatcatcc agaaaattaa caaaaagtcc
     6061 agcgaaaccg tgcgtctcca ttccttatgt ttctttgtaa attggacaat gacacgagcc
     6121 aacatgagga tataggatat cttcataaat tctgacggtt gaaatagggt aattccattt
     6181 attgataccc agtttttggc acccgttgat gcaactaagc ttggattata aaatacaatc
     6241 ggcaagatca taagtcccaa gcctaaaata tatagaaagg gggtcacctt ccaaagaaat
     6301 tctgtattaa agagcatgac cacaaaacca atcacaagcc ccaaggcaat ccaggcgacc
     6361 tgctgcccta aaatgggcag aatattattg ggataatcat gactaacggc tatatagata
     6421 gccaccacac cgatgaccag tagaaaaaat actggcaaga gcaaactata atcaactcta
     6481 gagtcgagag aacgtttcat atatactaac cttatacttt catacaatac tatttatcaa
     6541 agttcattaa aaaatctatc aatagcctcg tcaacttcgg atcgagagat ggttttaata
     6601 gtcgcttctt ctgctagaga tgctactatt tgtttgccgt atcgttttcc gatgattctc
     6661 ctatccaaaa taagagttaa ggaacgttgg tattcacgtc tcatacttct tcccaaagcc
     6721 tgttttaaac gaataatggc cattggcaat tgataatcat aaaaggcatt tttcccttct
     6781 tgattcagtt cttgattaat ctttttcgtc aagggttctt gaggattttg gaaaggaagc
     6841 ctcggtacaa cttgaatcac aaaaggatgg cttgaaaaat caactccctc ccagaaactt
     6901 gctgcaccaa gcaagatttg ttgttcacct ttttcaaagc gtttctttag ctgatgaaca
     6961 tccccatttt tatactgggc caagtggcta actgtaagta aatccgatac tgctagaagc
     7021 atgtctttag cggtaaagag aaccaaaatc ggttgctgga aagcttgaat ttccactagt
     7081 aaagcagcta cctctctggc atagacttct aaggaggttt ctgttaccag agggaaatct
     7141 ttgaccataa ccacttcttg ttcctgtttt ccccgagatt caatcttgac aaatttggct
     7201 tcaggatagc ctaaaaggtc tgccaaagaa accctctgac taatctcaag agtagccgat
     7261 actcccaaga cttggcaact ctcaggaagt aaagaggaca gaagagtacg gcatgatttt
     7321 gtagaagaaa tctgaatttt cttttgactc gtttcagtta cttcaagcca gtaatcacct
     7381 tcagctgtaa aatagcgaac cagctcatca aagccttcta cttctaattc tgaaaaatac
     7441 tgatggagat tgtccagaga atctaagata tttttcctag atttaccaga ctgaaattgt
     7501 tctatcaagt agagacactc aaagcgaata ctttctagta tccgttgttg aaccctgttt
     7561 tcttctccta ctaaagcctt atcaattaaa tcgataatag attgtatatc gtaggtctct
     7621 tgaagcagat tttctagagc taacaaaatc ttttggactt catcaataat cagtaaacgg
     7681 tcactgacaa attcaggatt atcttcaagt ctggtcacga gataggcatg attagtcact
     7741 aaaagcttgc aagtctctgc cctttcttga ctacgtttcc aaaaatcttc cgtcacaaat
     7801 aagctctggg atgataaatt cccatcatga cgaaggtctg ctagaaaatg ttggtaacgg
     7861 tagagttgcc cgatttcatc caaatctcct gtctctgtct cagtaagcca gaccaagact
     7921 tgcattttaa agcgtctaaa taagcgattt tcatcatttt cctgcaagga atgataaaag
     7981 gcatccaact tcagataatt ttgtggtccc tttaagctat gaatatctgt atggaacact
     8041 tccttgaggc gtttaccttc ttcttccatg atttgatttt gaagaatctt tgtcggaaca
     8101 ctaagaacaa tttgtcgctc tttggattga gataaagcgg gtaagagata gccataggtt
     8161 ttcccaatcc ctgtcggcgc ttgaatcaga gagacaggtt catctttcaa tagcaagcca
     8221 acctctttag caaaactttc ttgttcctcc ctcacttcaa ggttcaacag agaaatattt
     8281 ttagaaaagt cttgagatag ttttcgtggc tccagagaag ctgccgtttt cttaaaatac
     8341 agaccttgaa cttggaccaa gtctggagaa ctcaggatag attggttgcg ataagtttcc
     8401 tcaataacca ggtaggactc atataggaga gcgtcagcca tttccagcaa gcgttccaag
     8461 agacctttag gaagctgagt catcttttcc cgtaaaaaaa gaagtaattc tgctgtagct
     8521 tgggcatctg aaagggctgt gtgtgcgtgt ttaagaggaa ttcctaattc tcgacacaaa
     8581 atcggcaagc tatatttttc cagttcaggg aaaaagacct gggccaattc gaccgtatca
     8641 acacgagggt ttcttagctc atagccttca aaaaataaat tttccgccaa gagattagca
     8701 tcaaactgaa cattatgggc tacaaaaatc ccatcctcca ccaagtcaaa tatttttctg
     8761 gcaacttgcg aaaaatcagg tgcttgcgcc agacgttggt ctgtcaatcc tgtcagttct
     8821 ttgatatgaa catccaaggg ttcatgtgga ttgacatccg tcgtatagtg atcgacgatt
     8881 tctccgtcct caatcacgac aattcccact tggataattt tagccttact acctgtgcta
     8941 gttgcctcta aatccaccac aacatagcga ttaccaatcg ttttcattat aaaaaattat
     9001 accaaaaaaa gggccatttg gcacctgcaa acataggata attttcaaac tcaagaacac
     9061 tacttttctt aaaaatccca tcgaatagag cattctagcc tgctgatttt tgtaaaatag
     9121 tgaagaaata cgaaaaattg agagaagaaa tgtttgcatc ttccttctct caacgaatca
     9181 tgtattttat ctaaccatgt caggatcgta gtcataaaat cctgtatcaa ggaaacggtt
     9241 tttagggtga agtttacgaa cttcttcatc cagcaagaag gcttgatcat ggctaaagtt
     9301 gccctcttga atccaattac gcgcttggat aaagagtttt gcaatctcaa caaagttctg
     9361 accaggagtg tagagccctt ctagtttcca gcgagtaaag ccatgctcca ccaattctgt
     9421 taatttgatc atcaaatcaa ggtcattgtt ggcaaagata tgggtcccat gattatcttc
     9481 aaaaatggaa tagtggctct ctggatcact tggctcagcc aagaagaggt cacgtttatg
     9541 cgtcttttca tcatcgatat gtgtaaagtt atagtagttt tgcaagagtg gacgtttaga
     9601 atgatggatg acgctagcac cgtaaaccaa aacttcagca ggaatttcca aaatctctgg
     9661 cattttgaaa agttcagctg atggaatttc acgcgccaaa acagcctcag atgcgccagc
     9721 cttttgtccc cagaagttaa tctgacgact gctagttacc atggttgaag catcgtagat
     9781 ggtcttaaat gaataaccat cgcggttaac tacgtaaaag acgcctgcat ccccaatcgt
     9841 aatatagtct gtcttgattt cttccaagaa gtctaagaaa ggcttgatac ggtccatcat
     9901 atcttggtgc atgagagcat tgaccgcaac gagcaattcc ttaccagcat catgaaccaa
     9961 cttagcgatt tcacgtaatt ggtcataact aaaggtcgtt ggcagacgaa ggccaaaatc
    10021 tttctcaccg acatagatac ggtctacgcc agcttcgagt agttgttcaa cttgttcaat
    10081 actttcagca gttgctgtaa tgacaatctt ttccataaga aaaattatac cacatttctc
    10141 caaaatcagc catgctttga aaatgaaaag ggactttatt ctttttgtaa cctttctgta
    10201 ataattctct gccgaaaaaa tgataaaata ggtatgtact gttaaggaga gaattatgcc
    10261 cgtaagaaaa ttacaatcct atgaggtaga ctatcaagaa gaattaaacc agcagcttcc
    10321 tcattaccaa gcttatacac ctgaagcgca atctgatgcc aatctcaagg aaatcctatt
    10381 ttttattaat atcgctgttt tttgtatctg tattgctatc tttagtttta tctttttagc
    10441 attaaaatta tcaactgctc ttgcctttgc cgcagcaatc ggattcagct tacttgtttt
    10501 aaaagttcaa cggtctatta tcaaacgaaa acgtagaaga taaatccaaa atatcgcccc
    10561 agttggagcg atatttttat tttttctttt tagatggtgt ctgaatggca atcttcactt
    10621 caaagattgt tcccttgggt ttattatctt tgacagtaac agttcctttt agagcatcta
    10681 caatttgctt ggctagggat aatcctaaac caaaaccacc tttttgccga gttctagcct
    10741 tgtctactcg ataaaaacgg tcaaaaattt tctttttatc ttctgtcgaa ataccgattc
    10801 cattatcaga aacaagtaaa taaagattgc gatcggtcgc cgagataaga aaatcaattt
    10861 caccatcctc ctcagtatac ttgacggcat tatcgaaaag aatggtcatc agttgtttca
    10921 gaagaagctg atctgtgaca attgttcgat ggatacgatt ttcaaaacgg aagacacgat
    10981 tattttccga agcaatcatc tcgtagtttg tgaaagttgt attaaaaaag ctagttggaa
    11041 cttctgcaag ctccggctta atcccatcat ctctccgagc taagttcagc aagcttgtcg
    11101 ttaaaaaacg catatttcgg acttcttcca aactcgatgc aatgctttcg ctcacatcca
    11161 taatggtagc ttctggctta cgaaaaaggg tctctaagcg attttgcaaa actgcgagtg
    11221 gagttcgtaa ctcatgactg gcattttcca caaaagactg ttgcttctgc atactctcaa
    11281 gcaggggcct aacactgacc ctagctagat agagactggc aagtaaagac aaaatccaga
    11341 aactagccat cacgaccaca atcaattgct catgcttttg actagcctgc tccaactgac
    11401 tggtattaat caagacagca gcatacttga tattggttga aaccgaacta atattggtct
    11461 ccatcaaaat cacacgatag atttcttcct gcccatagct attaaaaacc tgaatctggt
    11521 agatatgtcc tagttctttc ttttctaact taatcttatc caagcccaaa aatcgatttc
    11581 cagaaagaag ttgagtaaag tccttatcaa agagaatgac ttccgtattg gaactgacat
    11641 taggttttat ttctacttta ctagcgtccg ccctagcatt ttctaaatct ttaatctctt
    11701 ctgttgccct atttatagcc agctgaataa ctgcttgagg attttcactc agtccatgaa
    11761 gcttatcgtc caccgaagta taaagactcg aatgcatgac ttgtaaaata atcagagtca
    11821 ttgtagaaaa aatcagggtg aagacaccga agttgcggat aaaataacta aagtcatccg
    11881 cataccatgt ttttttaagt ttactgaaca tcttttaaaa gatacccaac actacgcaag
    11941 gtttgcaaat tctctgcaaa agtggttccc tttaatttct tacggacttt tgaaacatag
    12001 acttcgacaa ccgaaatcgt tgtatcacta tcaaatcccc atagacggtc aaaaatctgc
    12061 gtcttaggca aaatcacatt ttgattttga aggaaataaa ctagtaaatc gaactctttc
    12121 cccagcaatt cgacaggagt atcttcaact ttaacggtat tggttgataa attaaccacg
    12181 atattcccat aagtcaaggt gttttcatta aacttccctg aacgtttgag aagggcctga
    12241 atccgcattt taagttcttc taggtagaaa ggtttggtca gataatcatc cgctcccagt
    12301 tcaaatccat gtcccttgtc atccaaactt tccttggcag tcataatcag aactggtgtc
    12361 gtaattccct tttcacgcaa ttcttttaag acttggaaac catttttttc tggcaacatc
    12421 aaatccagca aaatcaaatc atagacacca ctctcagctt cgtagagacc ttcttctcca
    12481 tcaaatacct gcataacatc cgcaaaatcg tctaaaaagt caaatactga atttgacaga
    12541 cctaggtcat cctcaaccaa taagattttt atcatgagaa actcctcctt attaaaacta
    12601 ttataccaaa tttgccttaa aaaaaactca actctctgca ttttacatga gatagctgag
    12661 ttttcttttt attttaggct tatttatgca tttccgtatt gaagaacaac tgcttcgact
    12721 gcagcttttt cacggctaat caagtcaaca cgtgctgcaa tttccttgat tcccataccg
    12781 atgttacggc taagagcaag gtcagaaagt tgcggctcaa agaactcctt gtattccgcc
    12841 aagcgttgct gagtcttaaa tacatgagca ggaaggataa caaagctatc aaagctcata
    12901 tctcctccaa gggctgcctt aatccaagcc cagttttcac gcgcccaaga ccaagctgtt
    12961 ttctgagttg cttgatgagc taggaattgg taataccaag cagacaagtc ctgtggtttg
    13021 accacaaatt tgtccttcca agaagtaatc aagttttgga tattatccgc atctgtactg
    13081 tatgcaagag ctgctgccaa ctggcgttta aagacagcat ctgttgcgtg agtataagta
    13141 tcaagataaa gtgctaacaa gtctttagtc tcatgatgtt tcatctcatt aatcagaact
    13201 tgtgaacgaa tagctgctgg gagtcctgca agattctcct tgtgtgttgc gaagatttgg
    13261 ctagcgactt gactagcttc tgcatcattt gagcgaatca tcatcgaaac agccagctga
    13321 cgaaccaatt catcctcatc tgattctccg tctttagctt caaaaccaag acggtcatag
    13381 ttatgacgag ccaatttagc aaccagtcct ttgaaggctg tttcagcatc cgttccttca
    13441 tcaataaagc gctcaagggc tgaaatcact tgagaaacag ctgaaaccac cagataagac
    13501 tcttccttag caagtttatc aagaactgga agcaagtctg cataagaaat gtgccctgcc
    13561 tcagccaaca aacgacgttc ttgaacaatt tgcagtttgc ttgtgttatc aagtgtctct
    13621 agctcagcaa gaacagcttc taacaagtct ccttgatagt cggtaatata gtgggcagta
    13681 ttttcagtgt tgagacgaag agctccttca ttttcagcaa gaagagctgc gtagccaggg
    13741 atttcgatac tttcagtttc gagtgtatca ggcaagcctt tccagttgct attgaggggc
    13801 accacccaga gacggttctt gtcttcgttc tcaccgatga agaattgttt ttgtgaaatc
    13861 ttcaagacat cattttcaac tttaacagta agaactgggt aaccaggctg ttccaaccaa
    13921 gaatccatga aggctgcgac atcacgtcct gacgcttgac caagggcatc ccaaaggtca
    13981 ctaccaatgg tgttgctgta ttggtgtttt tcaaagtagg cgtgcaaacc tttagcaaaa
    14041 tcagcatctc ctagccaacg gcgaagcatg tgcatgagac ggcttccttt ggcatagacg
    14101 atagcgccgt caaagagtgt attgatttca tctggatgtt taacttcgac gtggacagac
    14161 tgaacgccat cagtagcgtc acgttcaaga gcaagaggta ctccacctgt ttggaaatct
    14221 tcaaagatat tccagcttgg ttcgatggta tccacacaga cgtattccat catattagca
    14281 aaactttcat tgagccaaag gtcatcccac catttcatag tcacgaggtt cccaaaccat
    14341 tggtgagcca attcatgggc cacaacaagg gcaacttgtt gacggctagc aaatgtagag
    14401 ttctcatcga caaccaagta aacttcacgg taggtcacaa gaccccagtt ttccatagca
    14461 ccagctgaga agtcaggaag ggcgatgtgg agagattgag gaattgggta cttaactcca
    14521 tagtaatctt cgtaaaactc gatagagcga acagcgatat ccagtgagaa atcaagattt
    14581 gaaagtggat gtgctttggt tgagtagaca cctaccaggg taccattttt agttttagcg
    14641 gtcacccctt gcaaatcacc agcaacaaag gccaacaagt aagaagacat gcgaggtgtt
    14701 gtctcaaact tccagatacc tgtttcctta cggttttcaa catcgatttc tggcatgttt
    14761 gacaaggcca attcaccttc tgcttggtca aagcgaagag agaggtcaaa agttgctttg
    14821 gcttcaggct catccacaca tgggaaagct tcgcgcgcaa aatggctctc gaactgagta
    14881 gacaagacct ccttcttgac tccatcaact gtataataag aagggtaaat ccctgtcatg
    14941 ttgtctgtaa ttttaccaga aaaggcaaga accaattcaa cttgaccagc ctcagccaat
    15001 tcgatatgaa gggcttcatt gtcatggtca actgtaaatg gacgagcttg acctgcaact
    15061 tctacagagg tgatttccaa gtctttttgg tggagggaga tgcggtcact ctgtgcttga
    15121 ccagtgatgg tcactttccc agaaaaagtc ttggtctcac gactcaaatc taaaaataaa
    15181 tcataatgtt caggaacaaa ttgcttaata aaatgttcaa ctgcttgcat agttttctcc
    15241 tattctaagt ttaagagctg ctgctcttct tcaaacaaac ttattatatc atattttgct
    15301 tgagaaaaaa ggattggaca gcgatttcca aaactttctt ccttctaata gtctacagtg
    15361 ggtagacctt gcgaaattct tctaaaacaa ccttgctttc aggtgtaaaa gtcagcccta
    15421 cttcacccaa acagtctgtg aaaaagtcct catgagcctg gcgccaatag gcaagtgatt
    15481 tgtctccctc accttcctta aaggcatgtt gcgcagagac ttgattgaag agttcaacag
    15541 aaacctttgt aatttcgaca atgcagacag actgattttg actatctaaa atgacatcga
    15601 aggtccctac ttggggaagt ggttcgtctt ctagtacata gaggtcatag gctgatgctg
    15661 ttgctgtctt ttctccttta aacaccaaat ccgctaaaag gtctggttca actccaaaag
    15721 cccaagcatc gatttcatct ccgatcaaag gattgatttg cttgtattta ttccacattt
    15781 cttgcggtat catgggtact cctttgtaat tttttacttt cttcttttat gtgtttaaga
    15841 tgatctggat ggtcaatctc taaatcaaaa atctctggaa tagaactgta gtggataatg
    15901 cacttgatac ccaactgatt cattttttgt atgaaagaag tattcagata gcctgctaca
    15961 gcaaaattaa tcttgttctt tcttgcttta tcctgcatat ctcttagcat atctaacatt
    16021 attggacttt ccatatcatg ccattgactg tttctcatag tcgcaaaaac aaaggaagtc
    16081 aaatcattca ttccaactac aatctttgaa atgcccgttt ccagtatact agataagtca
    16141 aaatacgctg acggtaattc aatcatcgtt ccgactttcc cagtaaagcc atactgacgc
    16201 aatactgtaa tagcttgttt taattggtcg gcatcattga cagaaggaaa gataacagat
    16261 agattggggt tggtttgata aacttctgta acgacatgtg cttcagcctg aaattcatcc
    16321 aaacacgcca gtaaacgtct ggttcctcta tagccaaaca agggatgccc ttcgtcaaaa
    16381 aactctctag tccccactaa acaattggct tctgtattcg ttaattcaga aaaacgatac
    16441 caaacttcct tacctaagta aaaggagcaa atagtatcaa gataatcttt tacaaattgc
    16501 tgacaacttg ctaatatgat gttttgattg agctctctca ataagtattc cccacgaatc
    16561 atgccgacgt ggtgaaatag ttgaggataa attttttcaa gaattttttc gccactaagg
    16621 gcaagttgat ttctcatagc cattcacctc gtcttaaaag atacaatatc atatataatt
    16681 atatcaaaat ttcctctagt tcaaaagctt cataaggttc agataactca tatcctaaaa
    16741 attctgctac ttttttggaa gcctcattat gagcatccca aagtggaaac atatctctat
    16801 ttaaactctc tagaatcatt gcagcaccaa gctttttagc aagtccattt ccttgttcgt
    16861 ttggtctagt tgcaacttcc acttcaactg ctttacggta aactaaccct gaggaaatcc
    16921 cagcaatcag ttcattattt ttaagaatca caaagccaaa tccacctttt aaaacaaaat
    16981 cctgatagga ctcaaaatcc ccctgtaaat cctgtgacca ttcttccgta gaaaagcagt
    17041 tataaatatg attatcaata ggcacaatat tgtaaccttc ctctaaatga gtaactagat
    17101 tatttaaaaa ttcaacttga aaatttgcct tatctttaaa agaatagcga gtaaaagaat
    17161 ttagccccac ttttcgatgc agaaaatctt gccaattagt attttctgaa ataataatct
    17221 tataatctaa accatatttt ctaacaaaat cttcccaaaa tctggaatcc aactcccctg
    17281 ctaggtatag aaaattccca acatcataaa agcttgttgt cctatcattg tttcggtaga
    17341 cagttccaat ttgagattta agaccataga gaaccatatt tttcttccat gttttaaata
    17401 gttccattat ccctctactt caccttccaa ttcatgtaag aagtcttgtc cagttctgga
    17461 aatcctaata attcagactt aaccttcaag actaatggcg atgcattttc ttctgtaatt
    17521 tcttgaatat ccatccaaat atatccaagt gaatcattcg caccatcaga cacagcttcc
    17581 gaagtcgtaa cttgaggtgc actctcattc atttcaacat catacaaggc catgacatgg
    17641 tgaaccataa aatttgttaa ctcttctctg acgaaaacat cgtagattcg aggattagag
    17701 tagcttctaa cagtaaatcc cgtctcttcc ataacttctc tagtcagcgt ttccgtcagt
    17761 ccttcaccaa gttgctgact gcctccaggt agatcatacc gatgttgata agggcctctc
    17821 gttttttcaa tgcaaagtaa ctttccattt tcaaagcaaa cagcgtagac tccaaagtgt
    17881 tttttgattt ccatccaact cctcctactt caaagaccag ccaccatcta ttgtcaagat
    17941 ttgtccttgc atggcgctcg cttttccact tgctaaaaaa agactaagct ctgctatttc
    18001 ctctggctca atccagcgct tgattggggt ttcactagcc acccagtcag ccaaaccacc
    18061 tggttcaaaa tccgcagcgg tcatagctgt cttgactgct cctggagcga taccaaagac
    18121 ctgaatccca gcttcagcat agtctagagc caactgcttg gtgaagccag ccaaggcatg
    18181 cttggatgaa gtataggcgt gaccacctcc acctgctagg ctagaagcaa tggaacacat
    18241 attgatgatg attccctttt tattttccag catttgtgtc aaataatacc gagtcaactc
    18301 tactggaata atgtagttga tttcaaaaat ctcttgaatg tcctgcgccg tttgttccaa
    18361 cagtggtttg taatcatcca aaactccagc agtattacac aaaacatcca cctgagggca
    18421 ccagtcaaaa ataggttcca agtccaaggt caaatctctc tgtaaaaagt gaaaatcacc
    18481 ctctaagagt ggcttttcac cttggtcaac tccataaact tgatagccct tctctaaaaa
    18541 gaggcgagct tgagccaatc cgatccctga actcactcct gtaatgagta cacgtttagt
    18601 catgcacttc tacccaatcc gttgccaaaa catcacaaac tgtcgggctc cacatggaaa
    18661 aaccttctcc ttcgccagaa acgttgatta ggaaataagg tgtcatttca agtgcaagcc
    18721 cattttgctc gatggtatca aagagttgga catagttttc cgcacctccc caaccagttc
    18781 gtacatattt tctcttagcc tttaacccag gcaggatctc ttcaaatgtc atgtttttct
    18841 cctttaattc tacattcttc atttaattat agcaaaaaac cgctttatac ggctttttga
    18901 atgtgagtta ttcaaacctg ctactactta cggcaaatta ttccctgcag caagataaat
    18961 ttcataccat tcttttcttg ttaagctaaa gtttgccgct tggctaactt ctctcaagtg
    19021 cttaggattt gtggtaccta caactgcctg catttttgct ggataacgca atatccaaga
    19081 aatggcaata gttgaagagg ttactccata tttaatagct aaacgatcaa gtacttgatt
    19141 taaagcttga aatttctcat ttccaacaaa attcccttta aaatacccga attgtaagac
    19201 agaccatgct tgaatgacca catcgtgtaa tttgcaatat tcaaaaatgc tgccatctcg
    19261 catagctgct tgactatctt ccatattaac atgaaaagct gattcaaatc ctggagtaaa
    19321 agccacactc aattgtagct gattaacagc taacggctgc ttgacatctt ttttaagcaa
    19381 ctccatcatc ataggatttt gattagaaac tccaaaatct cgaactttac cttgtttata
    19441 aaggagatca aaggcttctg ctacttggtc agattccatc aaagcatctg gtcgatgaag
    19501 gagcaagcta tctagatgat caatcttcaa tctttgcaaa ataccgtcta ctgattttat
    19561 aatatagtcc ttagaaaaat caaaataggt aaattcttca atgcgaatgc cacatttgga
    19621 ctgaatccac atcttttctc ttaaatctgg acgatttttt aggacaagac ctaacagttc
    19681 ttcacaacga ccacgaccat aaatatcagc caagtcgaag gcattgattc caacagaaag
    19741 tgctgtttct acaaactctt caacttcttt tacagactta tcttttattc tcatcattcc
    19801 gagaacaatt tctgataatt ctttgtcatc ttgaccaaga gttatgtatc tcatcaaatt
    19861 tttctccttt aatttctaac attcttccct tcattataac aaaaaaccgc tttgcaacgg
    19921 ctttttgact atacttcact ccattttatc ttcttaaacc catggaacaa gagaaaaatg
    19981 ccaataaaga ggacagctaa aggaataact tttgtaagga aaacatttga aattcccatc
    20041 cactcataat aacggagcag agaacccacc acaagatggg caataatcat actgacaaat
    20101 ggacgaaaga ccgcttcttt ccaattccaa ataccgataa ctagcgaaat cgtaaagaca
    20161 gacaaactat cccagggatc cggaatataa aaggctcctt cttgtatgaa gcttgccatt
    20221 cctacatatc ctaaaacaac tagaagaact atagtcccaa caacaatgta agtgccaaat
    20281 ttcattttag gagaatcttg gactaaactt cttcgtaaaa ttgtggccac aagtccaaat
    20341 ccaatcagaa aaataagaag ttgccctaaa aatgtgagca aattgactgt taagagagga
    20401 cctttagaaa aatcacttag tagttgataa taacgtaata ccgccaggac aagaattggc
    20461 gtcaaaaggg actctttgat agaactgcga ggtgctccct tgagaatctc tttcattatt
    20521 tttttaggat tcttacctag ataatcctct gcactcatgc catctcgttc tgcttctgag
    20581 aaatctagca tcatcaaata gatctgctct ctgagatagt cttcatcata gagaaatcca
    20641 gcaagattaa aactttccca caactcctca aaatactttt gattctcctc agaaaactca
    20701 tgtagcaaag cgcttgtttc ttcgtaatac ttcattttct tcatggttta acccccattc
    20761 ttaatccctt ctactttttg actcaaatcg tcccattgtt gccaaaagac tgagacacgc
    20821 tcttctcctt ctttcattaa tgaaaaatac ttccgatctg gaccatctgg cgacgggcgc
    20881 atgtcgcctc ttatccattg atttttttct aacttttgca acaaaggata aatagttcct
    20941 ggaacgatag tatcaaatcc agcctctcgc aaagtctgaa ccaactcata accataccgc
    21001 tctttttgac caatcatatc caagacacaa ccttcaagaa caccttttaa tagctgagtt
    21061 tctttcatca cttctccctt ctaatctatt ttgtaatacc tactagtaac ttcacctata
    21121 gtatattact tctacactag tttgtaaagc ataatagtta atactcttcg aaaatctctt
    21181 caaaccacgt cagcgtcgcc ctaccgtatg tatggttact gacttcgtca gtttcatcta
    21241 caacctcaaa aacatgtttt gagctgactt cgtcagtttc atctacaacc tcaaaaacat
    21301 gttttgagct gacttcgtca gttctatcta caacctcaaa gcagtgcttt gagcaacccg
    21361 cggctagctt cctagtttgc tctttgattt tcattgagta taaataaaaa aacagaacta
    21421 gtctgaacta gtcctgtcta cttttaccca atcacacttc catttggtac agctggatca
    21481 actgtgagaa gggttaattt gccatcatgt tcagctgaga gaatcatacc ctggctgaca
    21541 tattttttca tcattttacg tggtttgagg ttagcaacga tttgaacttt cttgccgacc
    21601 aattcttgtt catttggata gtattttgca attcctgaaa gaatctgacg atcttctcca
    21661 tcaccagcat ccaagcggaa ttgaagcaac ttatctgaac cttctacttt agacacttct
    21721 ttgacttctg cgacacggat ttcaaccttg tcaaagtctt caaacttgat ttcatccttg
    21781 tttagtttga gctcaacttc gtccggattc cattcttttt cgactgctgg tttattgcct
    21841 tccatttgtt ccttgatata ggcgatttct tcttccatat ttagacgtgg aaagataggt
    21901 gttcctttgg caactacagt cacatctgct gggaagtcag ccaaactcaa gttttcaaga
    21961 ctagaaactt cttccaaacc aagttgagtc aaaactgcac gactagtttc catcataaat
    22021 ggttcaatca agtgagcaac tacacgaatg ctggctgcca agtggctcat gacacttgcc
    22081 aattggtcac gaagagcttc atccttggcc aagacccatg gtgcggtctc atcgatgtat
    22141 ttattggtac gagagatcag agtccagact gcttcaagcg cacgtggata gtcaactgct
    22201 tccatgtgtg tatggaagtc tgcgattgat tgttctgcaa cctcagcaag aacatgatca
    22261 aattcagtca caccttctac ataggcaggg atttgtccat caaagtactt attaatcatg
    22321 gaaaccgtac ggttaaggag gttcccaagg tcattagcca attcatagtt gatacggccg
    22381 acatagtctt caggagtaaa ggttccgtct gaaccaactg gaaggttacg catgaggtag
    22441 taacgaagtg gatctagtcc ataacgctct accaacattt cagggtaaac gacattccct
    22501 tttgacttag acatttttcc gtctttcatg acaaaccaac catgggcaat caaacgatca
    22561 ggtaatttaa catccaacat cataagaagg attggccagt agatagagtg gaagcgaagg
    22621 atatcttttc ctaccatatg gaagactgtt ccattccaga acttgtcaaa gttaccatgt
    22681 tcgtcttgag cgtagccaag agctgtcgca tagttaagaa gggcatcaat ccaaacgtag
    22741 acaacgtgtt ttggatttga tgggacaggc actccccatg taaaggttgt acgagatacc
    22801 gccaaatctt ccaagcctgg ctcgatgaag ttgcgtagca tttcattaag gcgaccatct
    22861 ggcgtgataa attcaggatg agctttgaaa aattcgacca aacggtcttg gtatttgcta
    22921 aggcgaagga agtatgattc ttcagaaacc cattcaacct catgacctga tggagcaata
    22981 ccaccagtca catttccagc ttcatcacgg aaaacttctg ccagctggct ttctgtaaag
    23041 aattcttcgt ctgatactga ataccaacca gagtattcac ccaagtagat atcatcttga
    23101 gcaagtaagc gttcaaagac ttgtgcgaca actttttcat ggtagtcatc agttgtacgg
    23161 ataaatttat cgtatgagat atctagtaat tgccagagtt ctttaactcc aaccgccatt
    23221 ccatcaacat aggcttgagg tgtaatacca gcttcttccg ctttctgctg gattttctga
    23281 ccatgttcat caagacctgt cagataaaat acatcgtagc ccatcaggcg tttgtaacgt
    23341 gctaggacat cacatgcgat agttgtgtag gcagaaccga tatgaagttt cccagatgga
    23401 tagtaaatcg gcgttgtaat ataaaaattt ttttcagaca taatttttcc tttccaggca
    23461 aatgaaacct gtttttctaa cacttcatta tatcacattt ttaatgaatt tcaataggga
    23521 aatccataca aaaacaagat agacgagtgt ccatcttgtt gatctcattc ataacgaagg
    23581 gcttcaattg gatcaagttt cgatgccttg ttggctggca agactccaaa aatcatacca
    23641 acactagccg aaactgcaag actaaatagg gcgactggga ttgatactcc aacttctata
    23701 ccttctatta aaccttgcag taacaaacct gctaaggcag ttaaaccact tgcaattgtc
    23761 aagccaatta agccacctaa caaggtcaaa atcatggatt caatcaaaaa ctgaattaaa
    23821 atattggcac gtgttgcacc caaagcctta cgaagaccaa tctcacgagt gcgctctgtc
    23881 accgaaacca gcatgatgtt catgacacca gttcctccaa caaagagaga aatccctgcg
    23941 atggaactaa taatcgtcgt cataaaacta aacgattgtt gaatttctgc aaatacaagg
    24001 gactcatctg ccacctggta ttctccctgt tgtaagcctg caagctctgt catttttcgt
    24061 gccagttctg gacccagagt tggggttaaa ctggtatcat tcactcgaaa gacaatacta
    24121 gctatttcat ctacattaaa attcgcagca agggagatat tggtagtaat aggcaagcca
    24181 ccaaacccat atatttttga tcttttagcc tccggactag tataaacccc aatgacccgg
    24241 taactaaatc cattgacttc tacaaccttg ttaatagcct cttgaggaga ttcaaataaa
    24301 ctaatggaca attcctcatc tagcaaaata acacttgcaa actctttgaa atcttgctct
    24361 ctcagactac gacctgcaat aatttcattc ttaacagcgt ccatgtaagt tctgtttcca
    24421 cctgtcaaat tagcattctc aaccttttta tcttgatagg tcaagatggc attcgttgaa
    24481 ttggttacat agtaactatc cactcccttc agtttagctg cctcttggac ccaggattct
    24541 tgcggttttg gcggttcaac aggaacttcc tcttcctttc cagaaaccgt aaaagctgat
    24601 tgtttctgag taaaagaccc atctttactt tttttaggag agaaaaagac gctaatattt
    24661 ttctgagatt tagtcatatc tttattgact tgacgagata gggaatcacc caaagccata
    24721 atcacaacaa ctgatgaaac accgataata atcccaatca tagtaagcaa agaacgcatc
    24781 ttgtgagcca tgatagatga aaaggcaaat ttcagattct gcatcttagt tttcctcctt
    24841 tcctaactga gcactgtcag acgaaatgac cccatcccga atgacaatct gacgtttggc
    24901 ataggcagca atctcaggct catgcgttac catgataatg gtttttcctt ctttattcaa
    24961 atcaaccaat aattgcataa tttggttacc tgttttggta tccaaggctc ctgtcggttc
    25021 atccgctagg ataatagaag gattgtttac caaggcacgc gcaatggcta cacgttgctt
    25081 ttgaccacca gataattctg aaggtaaatg gtgactacgt tctgtcaatt caaccttgtc
    25141 taaatattcc tcagccaact tgcgacgttt tgaagacgaa actcctgcgt aaatcaaggg
    25201 caattctaca ttttgcagag cattgagctt cgatagaaga aagaactgct gaaagacaaa
    25261 accgatttgt tggttacgga ccttagctag ttgtttttca ccaagcccag ccacttcttg
    25321 accttcaaga taatattctc cactggttgg tgtatccaac atgccaatcg tattcatcag
    25381 agtggactta ccagacccag atggtcccat gatggctaca aattcaccct cattcacttc
    25441 tagattgata tttttgagaa cctgcagttc ttggtcacca ttacggtaac ttctgaagat
    25501 attttttaga ctaattagtt gcttcatcag ccttcacctc ttttccttct tccaaggaag
    25561 atgttggatt actgatgacc ttagcaccgt tcgttaaacc agaagtgatt tcttgatttt
    25621 ctgcgtcagc atttcccaat gaaacctcaa cttttttagc cttttgttgt tcatccacaa
    25681 tccagacata atttttacta tcatccatta ctagactgct aacaggaaca agaatagcct
    25741 tagttttgct tttaacctca atgttgacag aaaaaccttg tttcaaatca ccaacctcgc
    25801 ctgtcacatc aatagtataa gggtatttag aacctgtatt attcccggct gctggactag
    25861 ctgcttcacc attgttttta ggatagtcag aaatatagct taatttccca gtccattttt
    25921 tatcaggata cactttagaa gtaaagctta cttcttgacc tacagaaagg ttggctagat
    25981 tgtactcaga caattctccc ttgacttgta aattttcatt gctgacaata tgaaccataa
    26041 cttgactcgc ccctgttgga gatttagaaa cattgctatt gacttcgact acagttccct
    26101 ctagggtact gagaacagtt gttgcatcca attgactttg agccttgctt aattgcgctg
    26161 cagcatctgc acgcgcatca cgggcatcac ccaattgagc atcaatagaa gcaacagaat
    26221 ttccagccac tggagttggg ctttgcaccg ttgcatcttc tcctcctact ggcgctggta
    26281 actgtggagc ctgagctgaa gcggcttcat ttcgtgcttg attgagttca ttgatatgac
    26341 gatctgcctt agctactgct cgactagctg aatcataggc cgcctgcgct tctgaactac
    26401 tgtacttgac taaagcctgc ccttcgctga ccttatcgcc cacagaaaca aggatttcat
    26461 ctaaatcacc cttactagca tcaaaataaa catattgttc attttttgct gttactgtcc
    26521 ctgacaataa aacagaggat gccacgcttc cttccttggc aacaacaaga tgagtaggct
    26581 catcttttag agcagtctga gaaggttgtc taaaaagtaa aatccccccg gcacccaata
    26641 caactacact cgcagcaccg attgctgcat acagttgcca ctttttagct ttaccattct
    26701 ttttcttcat aatgaaactc cttttctttt ttacaatact ttgctattat accaaatttc
    26761 cctccagcaa acaatgcagt tcagatggaa ataatggcag ttcaggatta aacaatcgtt
    26821 cggaattttg cttttctatt gtactagata tataatacac tatcttgact tatttggcaa
    26881 gcttttttgc aattttttgt gcgtagcaga tttttgcaat caaatcaaaa aaagaataaa
    26941 atataactat caaaaaagac gctctactat ttaaaagatt acaaaggagt tttcaatgag
    27001 agaatatgat atcattgcta tcggtggagg tagcggagga atcgctacca tgaaccgtgc
    27061 tggtgaacat ggagccaaag cagccgttat tgaggaaaag aaattaggtg gaacctgtgt
    27121 caacgtcggt tgtgttccta aaaaaatcat gtggtacggg gcgcaaatcg ctgagacttt
    27181 ccatcaattt ggagaagact acggctttaa gactactgat cttaactttg actttgcaac
    27241 cctacgtcgc aatcgtgaag cctacattga tcgcgctcgt tcttcttatg atggtagttt
    27301 taaacgcaac ggtgtagact tgattgaagg tcatgctgaa tttgtagatt ctcatactgt
    27361 aagcgtaaat ggtgaactga ttcgtgctaa acatatcgtg attgctacag gtgcccatcc
    27421 aagtattcct aatattcctg gtgctgagct aggtggctct tctgatgatg tatttgcctg
    27481 ggaagaactt ccagagtcag ttgccattct aggcgctggt tatatcgccg ttgaattagc
    27541 tggcgtactc cacacttttg gtgtcaagac agatctcttt gttcgccgcg atcgtccttt
    27601 acgtggtttt gattcctaca tcgttgaagg tttggtcaag gaaatggaaa gaacaaactt
    27661 accacttcac actcacaaag tccctgtcaa gttagaaaaa actactgacg gcattaccat
    27721 tcatttcgaa gatggtacta gtcacacagc tagccaagtt atctgggcta caggccgccg
    27781 tccaaacgtt aagggcttgc aacttgaaaa agctggagtg actctgaacg aacgtggctt
    27841 tatccaagtg gatgaatacc aaaatactgt tgttgaggga ctctatgctc taggtgatgt
    27901 aacgggcgag aaagaactga ctccagttgc aatcaaggcc ggacgtaccc tatctgaacg
    27961 tctctttaac ggaaaaacta ctgcaaaaat ggattactca actattccaa ctgttgtctt
    28021 ttcacaccct gctatcggaa ctgttggttt gacagaagag caagctatta aagaatacgg
    28081 tcaagaccaa atcaaggttt acaaatcaag ctttgcatct atgtactctt cttgcactcg
    28141 caaccgtcaa gaatcccgtt tcaaactcat aacagctggt tcagaagaaa aagttgtcgg
    28201 acttcatgga attggctacg gcgttgatga aatgattcag ggatttgccg ttgctatcaa
    28261 aatgggagca accaaggctg actttgatgc aactgtggca attcacccaa ctgcatctga
    28321 agaatttgta accatgcgtt aatcgaaaca aaagaagctt atataaatat gtctgcttct
    28381 tttttaatga ttttcgaatc aacaaaaaaa ttatccaact tgactagtca cgaaaatcgg
    28441 ctgttacagt ttttgttacc ttgtttttaa aaaatggttg acatcgactc ctatatgagt
    28501 ataatagtta ctatacatta gaaaagaggt taactatttt gaaaaaagct cacgtttatg
    28561 ctatccctgc tattggggct gctctcattg ctgttttggc acaaatcagt cttccaattg
    28621 gacctgttcc cttcactctg caaaactttg caatcggctt gattgctact gtctttagac
    28681 cgagagaggc tgtactttct gctggactct atcttcttct aggtgctatc ggtcttcctg
    28741 tctttgcagg aggtggagct ggttttcagg ctttagttgg ccctactgca ggctatcttt
    28801 ggttttatct cgtttactct ggacttactt cctctctaac caacagcaag agtggtgttg
    28861 ttaagatttt tcttgcaaac ctcttgggtg atgcccttgt ctttgtcggc gggattctca
    28921 gcttgcattt cctagctgga atggcatttg aaaaagctct tgttgtgggg gttcttccct
    28981 ttatcattcc agaccttggc aaacttctgg ctattagttt tattagccgt cccctacttc
    29041 aacgccttaa aaatcaggct tactttacta actaaaaaag gataccgagt tatcatgact
    29101 caatatcctt tttttatttt gaaaacttat actcaatgaa aatcaaagag caaactagga
    29161 agctagccgc aggctgctca aaacactgtt ttgaggttgt ggatgaaact gacgaagtca
    29221 gtaaccatac atacggcaag gcaaagctga tgcggtttga agagattttc gaagagtatt
    29281 agttgccttt aaaggcatcc accatcgttt gaaattcttc atttgagaga gtaatccctt
    29341 tgcccatttt agtatggtct ggactccaag cacgaatatc aaactttgca gaggcaccat
    29401 taaagctcac acggttaatt tccttggtcc aacctttttc gttttcagaa agagtcaaca
    29461 agtgctcttc gatttcaaat gtaaattctg ccattttctt ctcctttttt agtttcatta
    29521 gtttattcgt aaaatcttgt agattttagg aaaattttat ataatattga tataaaagaa
    29581 gggaggccaa tatgagacat aaattccagc aagttctaaa taaaatacat gattttttaa
    29641 atggatatga ccaacctgac cagactgaaa ccaactccct tacagccact attgaagagg
    29701 ctatccagaa acaaaccgct gttcacctta tcttatctga gacaagcttt acaggtgaca
    29761 tcatcaaata tgatcagcaa ggccagcaaa ttatcgtgaa aaatttttcc aaaaatgtga
    29821 gtcggattat ccgtataagc gatattcaac gcctgcgatt tgtcccctca actgtccaaa
    29881 cagcccaaaa aaatagattt aagaaagagt gagatgtagt tgcttcatcc cactcttttt
    29941 tcttagcgaa tttgttcaaa atgtaaatga actgcaatat gatctccata accacttctt
    30001 tccaagtcac gttgtaaacg ataggaaatg tagtgttctg caatggtaat gtaacctgcg
    30061 cccaataaac gatgttcaac catagattga atcatactga tagtcgcacg ttccaccttg
    30121 gcttcttgta aatccaaaac taccttctta gtgacttgag caagattttg acgcaaatca
    30181 tctgtcaaaa cataaacagt ttgggctgcc ttcaagatgg cttggtaaat cttatctgga
    30241 ttaaattcag caatttcgcc attacgtttg attacttgca taggtttctc ctttattctt
    30301 tgttttcttt gatttctgcc agcatttttt cttcttctac tgtcagttga taatgttcaa
    30361 gtaaatccgg tctgcgctcg taggttttct ttaaactctc gtacaatcgc cactgacgaa
    30421 tcttttcatg gtggccactc atcaatacat ctggcacgac catgcctcga taatcatagg
    30481 gacgtgtgta ctgaggatat tctaaaagac ctgaagaaaa actatcatct tggtggctag
    30541 actccttgcc aatcacttct ggaatcaggc gaactgtagc atcaatcatg gtcatagctg
    30601 ccaattctcc accagtgagg acatagtcgc ctagggaaat ctcatctgtt accaaggtct
    30661 taatgcgctc atcataaccc tcatagtgcc cacagataaa gattagctct tcctcttgag
    30721 ccaaatcttc agcataagcc tgatcaaact gctttccagc aggatcgagg agaataacgc
    30781 gcggattttt cttttcaata gcatcaaagg aatcgaaaat aggttgtgct ctgagcaaca
    30841 tgccctgacc gcctccgtag ggctcatcat ctacatgacg ggccttttca gcattttctc
    30901 gaaaattatg atactggata tccaagagcc ctttttctcg agcctttcca acgattgagt
    30961 gctccagtgg agaaaacatc tctggaaaga gggttaaaat atcaatcttc atcgtctaac
    31021 ccttctaaga tttccacatc gacccgttta tttggaatat caacattgag aaccactggt
    31081 gggatataag gtaaaagcaa atcacgtttg ccttttcgtt tgaccaccca gacatcatta
    31141 gcacctggtt gcaggatttc cttgatggtt ccaaccaagc tatcaccctc atagacttcc
    31201 aaaccgataa tctcgtgata gtaaaattca ccatcgtcta ggtcattcaa atcttcctca
    31261 gcgaccttga gactgtatcc cttgtacttt tcgatagtat tgatatggta catatctttg
    31321 aatttaataa tgtcaaagtt cttctgttta cggtggctag cgatggtcac tgtttggaca
    31381 aactgatctt tttcatcaaa caaagccagc tcagctcctt ttttaaaccg ttcttctgca
    31441 aaatccgtca cagacaagac tcgcatctca ccctgtaatc cctgcgtatt aacgattttc
    31501 ccaacattaa agtagttcat cttgtctcct gtaatctcct tttttccatc ttattctgac
    31561 aattctcgaa taatagccgc aattttttcc tattctgacc attgtaaata atggtgattc
    31621 cttcctaaaa tgagtttagt attggaagtc caatattctg attctctgta ctctttttct
    31681 ctataagtct gacaaaaaat aaatacaggg acatgagatt ctatcgacaa attctcaaaa
    31741 tcttcctcag taatctctcc agatatctga aattctggat cttgattttc caactctaag
    31801 cctttttctt gcattaattc ccagattttt ttattcgttt caggactaaa tgttgcttga
    31861 gttaagttct taaaataaag ttcaggacca cactcgtcaa tcagcctcat ctgctcttcc
    31921 atttctggat aagaattttc taaaaaatca gcaaacatga ctttttagtt gtcggttcaa
    31981 ttgctactaa agtctgacgc ttaattggtt tctcgagtaa tttgcaagct aaaattccac
    32041 tccaactatg tgcacaaagt atatattcag aaattcctaa ttcttcaagt acttcataaa
    32101 ccgcatctgc aagattatct agattttttc cagcttggtc atgaatcgga ctcctacctg
    32161 tgttcggaaa atcaattgtc aaataaccaa ttgtaggagg aggtttttca agtataagtg
    32221 aaaaattttc ataacttggt agcaaacctg ctccgtttag acaaactagc actttctttt
    32281 gcttttgata agcaacagag agactaccaa tttctgtaga tacttcaaac ctcttcataa
    32341 agaaatccac tcattctata taatgaatta ttaaaaatcc ttatctttta ttttatcacg
    32401 ttccaaggat tttctcaagt tggaggaagg ggacaacatc tctactttcc cttcaaaaat
    32461 ccttccaaat catgtttatg ttggtaatta acggctgcgg ttttgtcttt ctcaaagaca
    32521 gtcttggtaa ggtcaatatg attaatagct acgattgcga cggtgtagta aatgatatca
    32581 gccagttctc tggcaagttc ctcgttcgaa tcctatccct tcttttcgac cagagcgcct
    32641 attcaaaacc tcggctactt ctccgacttc ctccactaac ttcataaaga gaccttcatc
    32701 agtccgagac tgctgttaat gttcgagtaa gtagtcttgg aattgcctaa acgttcaatc
    32761 ttttatagta tattgaaact agaatagtac acctttactt ctaaaacatt gttagaaatc
    32821 gatttgactg tcctgatcga tttgtcctgt tattatttca ttttactata tcttctattc
    32881 cacacaaaaa agcgagacat ccgtcccgcc cttcttattt ttcgtcaata acgattctta
    32941 cttttttgta ttcagttggg acagagtaga caatcgttct tatcgcagaa atagtgcgac
    33001 ccttacgacc gattacacga cccacatcgc tttgatcaag attcaaatga tattccaaaa
    33061 attctggtgt atcttcaatc ttgatagtta aggcatctgg ttgtgaaatt aagggtttca
    33121 caatcgcaat aatgagattt tcaatcgtat ccatctgtca acctacttta aacttatttt
    33181 gagaatttag aatcgtggaa ttttttcaat acgccttctt ttgaaaggat gttacgtact
    33241 gtgtctgaag gttgagctcc attagccaac catgcaagaa cgcgatcttc tttcaaagtt
    33301 acttggtttt cagcaacaag tgggttgtaa gttccaactg tttcgatgaa acgtccgtca
    33361 cgtggtgaac gtgaatctgc tacgttgata cggtagaaag gttttttctt agaacccata
    33421 cgagtcaaac ggattttaac tgccattttt aaagtctcat ttctttaatt ttttatttcg
    33481 gtgaaatagc tgagctattt agcacatgtt ctattatagc agatttctgg catgtgtcaa
    33541 gaaaaaaact tgacagacta taaatttctt aaagtattta gaaatttgtt agaataggaa
    33601 ataaatgttt gaaagatgct atccgtgaat actattttag aaaaatttta taaagaacac
    33661 caagtcaaac ccttcatatc gcctgaacga gagttggata cttggcttct aagtcccaag
    33721 cccgttccca agcgaaatat ggacttatta gcagatgatt cactagcagg agatatcatt
    33781 ttactatgga gaatccagtt tggtactttt accactgaaa catgatttta aaaactccca
    33841 ttcgaatatg attcaaaatt cgcttagaat caatgttttc ccactttttc aaaaaataaa
    33901 ttttcatcta atttaaataa ctttcaatta agtgtgaatt ttgcttaaaa tcattaaaaa
    33961 acaccccatg gtgtgaacca tgaagtgttg taaatgctgt gttgtagcta gttcttagcg
    34021 tttagagaac tggctagctt tacgagcttt cttaagatct ggtttccaaa atactttgag
    34081 tacacttatg gtattgatgc ccctaaacat ctaaaaactc tggtagaaaa agggtatgct
    34141 cttgtcgaaa cttcttttga ctcacttgac catctaaata ctaccatgaa aaagaatatc
    34201 ctgaagaaga agggagttac tggactttct aaaatgaaat cttgcgacct ggatcaagcc
    34261 cttcatgagc atttttcaga agaagaatta gctggtcact ttcatgtcct tctatggact
    34321 tttttacaat ggcattgcta tcacacccaa tacctatcta agcgcctggt tcgtaaactt
    34381 tattgcagct cttcctctaa atttcctaat tgttgaacca attgcccgtt ttatactaag
    34441 ttcttttcag aaaccattta ctggggaaga agttgaagaa gttgaagatt ttcaagatga
    34501 tgatgaaatc ccaactatta tctaagccag ttctgtaaac tactaatatt tgaaatccac
    34561 ttccttttag ggtacaatgg ttataaatga atttttgaga ggatcagaat gaaaaaacta
    34621 gcaacccttc ttttactgtc tactgtagcc ctagctgggt gtagcagcgt ccaacgcagt
    34681 ctgcgtggtg atgattatgt tgattccagt cttgctgctg aagaaagttc caaagtagct
    34741 gcccaatctg ccaaggagtt aaacgatgct ttaacaaacg aaaacgccaa tttcccacaa
    34801 ctatctaagg aagttgctga agatgaagcc gaagtgattc tccacacaag ccaaggtgat
    34861 attcgcatta aactcttccc taaactcgct cctctagcgg ttgaaaattt cctcactcat
    34921 gccaaagaag gctactataa cggtattacc ttccaccgtg tcatcgatgg ctttatggtc
    34981 caaactggag atccaaaagg ggacggtaca ggtggtcagt ccatctggca tgacaaggat
    35041 aagactaaag acaaaggaac tggtttcaag aacgagatta ctccttattt gtataacatc
    35101 cgcggtgctc ttgctatggc taatactggt caaccaaaca ccaatggcag ccagttcttc
    35161 atcaaccaaa actctacaga tacctcttct aaactcccta caagcaagta tccacagaaa
    35221 attatcgaag cctacaaaga aggtggaaac cctagtctag atggcaaaca cccagtcttt
    35281 ggtcaagtga ttggcggtat ggatgttgtg gataagattg ctaaagccga aaaagatgaa
    35341 aaagacaagc caactactgc tatcacaatc gacagcatcg aagtggtgaa agactacgat
    35401 tttaaatctt aaaaaccaaa aaaaatacag tatccacatt cggtactgta tttcttttac
    35461 tctcattctt aagttaaatt attaaaatcc catatttggt ctatccagcc ttcataaaag
    35521 tctagctcgt ggcagaccat aaggatagat cccctatatt ctttgagagc gcgtttgagc
    35581 tcatcctttg catccacatc caaatggttg gtcggctcgt ccagcactaa aacgttgttt
    35641 tcacgattca tcaagagaca gaaacgaacc ttggcttgct ctccccctga taatacttga
    35701 atctggcttt caatatgttt ggttgtcaaa ccacaacggg caagggctgc acggacttct
    35761 gcttgattaa gggcaggaaa ggcattccag acagcttcaa gaggagtttg gcgattaccg
    35821 ccttctactt cctgttcaaa ataaccaagt tctaaataat ctccacgctc cacttcccca
    35881 gcgattggcg agataatgcc caagagactc ttcaagagag ttgtttttcc aataccatta
    35941 gcaccaataa tcgcaacctt ttgattgcgt tcgaaggtaa gatttaaagg cttagtaaga
    36001 ggacggtcgt aaccaatttg caagttcttg gcttggaaga taaagcgccc tggtgtacga
    36061 gctggtttga aatcaaagga tggttttggt ttctcacttt ggagttcgat aatatccatc
    36121 ttatccaatt tcttttgacg agacatagcc atattacgag ttgcaacacg ggctttatta
    36181 cgagccacaa agtccttgag gtctgcaatc tctttctgct ggcgttcgta ggctgcctct
    36241 agctgagatt tcttcatagc ataaacttct tggaactggt agtagtcacc agagtaacgc
    36301 gtcagctgtt gattttccac atgatagaca atattaataa cgtcattgag gaatggaata
    36361 tcgtgcgaaa tgagaacaaa ggcattctca tagttttgga gatagcgctt gagccaatca
    36421 atatgctcag catccaagta gttggtcggc tcgtccaaca gcaagatatc aggcttttca
    36481 aggagaagtt ttgccaaaag caccttggtt ctttgcccac ctgacaaaga agttacatcc
    36541 gtatccatgc caaagtccat aacaccaaga gcacgcgcta cttcgtcaat cttagcatcc
    36601 aaggtataga aatcacgact ctccagacgg tcttgaagtt ctcctacttc ttccatgaga
    36661 gcatcaacat ccgcgccgtc ttcagccatt ttcatataga ggtcattgat acgagcttca
    36721 gctttgaaaa gctcatcaaa agccgtacgg agaacatcac gcaccgactg tctttcagca
    36781 aggacagagt gctgatccaa gtaaccagcc gtcacatatt tggaccactc aacctttcct
    36841 tcatctggca gcattttacc agtcacgata ctcataaagg ttgattttcc ttcaccattg
    36901 gcaccgacca ggccgatatg ttctcccttg aggagacgga aggacacatc ttcaaaaatt
    36961 gcacggtcac caaaaccgtg actcagattt ttaacttcta aaatactcat tttaattcct
    37021 tatcttgttt ttatgtaatc gtttataaag gagccaagcc agatagccac ccaaagtgtt
    37081 ggtccacaaa tcatcaatct caaagacgcg attgaaatca aagaaaaagt ctaagattaa
    37141 ttgcgtacac tcgattccaa gactcacaag aaaactaaaa agaaggacct tttttgtttt
    37201 ccgcaaattt ggaaatagat aaaggagttg gaaaatcaga ggaaaaaaca agaagacatt
    37261 gaggatattt tgtaaaaaaa tccaacataa ttgtccaatg tcactcactt cgcccagttt
    37321 ccagagagaa ttgaaaggag tcaaaagaaa aaccaggcgt ccaagatgct gaatacctgg
    37381 agttcccact cccacggtag attgttcttg aggagtaaag caaaaacaga caatgcaaat
    37441 gctatagaaa atgactcccc agaccaaaat atgattataa gtcttcttca tcattaagga
    37501 tttaccgctg cgactgcctt ctggcggtca cgtttcattg tgttagagcg caattgtcca
    37561 caagctgcgt caatatctgt accatgctct tgacgaacca cacagttgac cccttttttc
    37621 ttaagcgtat catagaaagc caacacgcgc tctttgggac tacggctata ttggtcatgc
    37681 tcactaactg ggttataagg aatcaagttt acataagaca atttcttgat gttcttgagc
    37741 aattcagtca attccaaggc ttgttctaca ccgtcgttga cttcattaag catgatatat
    37801 tcaaaggtta caccacggtt tgttgtctca atgtagtatt caatagcagc aaagagtttt
    37861 tcaatcggaa aggcacggtt aatcttcatg atacttgaac gaagttcatt gttaggtgcg
    37921 tgaagagaca cggcaagatt gacctgaacc ccttcatcag caaagtcacg aattttatga
    37981 gccaaacctg aggttgaaac cgtgatgtga cgagcaccga tagccattcc tttatcatca
    38041 ttgatagtac gaaagaaatt caagacattg ttgtaattat caaagggctc accgattccc
    38101 atgacaacga tatggctgat gcgttcatcc tgaccacgct catcaaagta tttctgaacc
    38161 agcatgattt gcgctacgat ttcaccgtta ttgaggtcac gttgcttctt aatcaaacca
    38221 gaggcacaga aggtacaacc gatattacag ccgacctgag tggtcacaca gacagataaa
    38281 ccatagtgtt gacgcatgag tacagtctca attaacatac cgtcgggcaa ttcaaagaga
    38341 tatttgactg taccatcagc agactcttgc acaatacgtt gtttcaaggg attgaccaca
    38401 aactggtcat tgagcttagc aatcaaatcc ttggaaaggt tggtcatttc ttcaaatgac
    38461 tgcacacgtt tacggtagag ccattcccag atttgatctg cacggaattt cttttctccc
    38521 tgctccaata cccattcctg catggtttga tgtaccaaac tatgaattga gggtttcatt
    38581 tcttctcctt attctctact cacttctgac gaatgacaaa atgacgttgt cccttgtcct
    38641 ctttctgaga atgctggtct ttctgacgac gtctattttt cttatctgca ttcgactttc
    38701 gtttagtttg agtcggtttc tttccttttc tagaaggtgt ttcttcttcc gtcttacgca
    38761 ttttcttgtc aaatgatgct cgcttagggg cttcattttc taagacaaaa taggcacaac
    38821 cataactaca atactctaaa aggtagtctt gtaaacgact gattttttca agtttttctt
    38881 ctgttcggtc atccttgtaa aaacctcgta ggcgaagctg ttcgttgctc cagtccccca
    38941 cgatataatc aaacttggtt aagacttctg aaaaacgctg attaaaagtc gtcacatcaa
    39001 aggcatcctt gatattttca accaaggaaa aagctatccc ttccgtttcg accttgtccc
    39061 cgtgtaaatg gaactccgga ccaggaaact tgttatagtt gtataattca ggtgcaattt
    39121 cttttcgcat agatatcctt tttccacgat tacttaatac tttattctac cataatttct
    39181 agcagttagc acgtttctca taaaaatgaa aaaagtctga cgattttgtc agaccagaat
    39241 cttataacct aaaaagagaa gaacaattct tccctccaac tatcattatt tagcagctgc
    39301 gtacaattca tctactttat tccagttgat tactgaaaag aaagctttga tgtagtcagg
    39361 acgcacgttg cggtatttca cgtagtaagc atgttcccaa acgtccaagc ccaagattgg
    39421 ttttttacct tctgagattg gtgtgtcttg gtttgctgtt gaagtcactt caagtttccc
    39481 ttctttgttg acaaccaacc atgcccaacc tgaaccaaaa cgagttgttg ctgctgcagt
    39541 gaaggctgct tggaattctt caaatgaacc aaatgttgca tcgattgctg ctgccagttc
    39601 tgctgaagga gctgttttct cgggagtcat caattcccag aaaagagcgt ggttcaagtg
    39661 tccgccacca ttgttgataa gtgcttgacg gatatcagct gggatagatt ctacatcagc
    39721 aagcaaggct tcaaggtctt caccgatttc agggtgtttt tctaaagctg cattggcatt
    39781 gttgacataa gtttgatggt gtttgtcatg gtgtaaatgc attgtttccg catcgatgta
    39841 tggttccaaa gcgtcgtatg catatggaag ttctggtaag ataatagcca tctgtaatac
    39901 ctctttttct ttctatatga aaatgataac gcaaacattc acttttttca agttttttgc
    39961 ttatagataa ggaaatcact gacatacttt ctaataaaat aagcaaatca gcaagaaacc
    40021 cacgacaaga taatcctgac gcgggttgta gtttcaacaa atgtcaattg acctgactag
    40081 caatctgtaa gagtgccttt tcaaaaagga aacctttttc ataaagacct gtcttaatct
    40141 gatagtctgt ctcaatcaaa taggaaatag cttgcttcag aaagctcaaa gaaagtcctc
    40201 tcgaatctct taatgcaaac ttgatttgat aaggatttgg gttacgtccc agataactac
    40261 ctaaactact tgcaatctgc gattctgttt ggccagactc cgccaaaatc ttcacctgag
    40321 taaaagtccg aaattgtccc agcatgactg caatcagttt gatttcatct tccccttgca
    40381 aggtcaagtc tctcaccaaa tcgcgcgcct gatccatctt tttagtcaga ataaactgag
    40441 ttaaatcaaa aatattgtcc tgcaaggtct tgggaattgc gttaacaata tcctcttcct
    40501 cgataacaga atccgcctta taggactgta aaaagagaag atttttctgg atttcgctaa
    40561 attgaaaccc cgacttgatg aggagatttt caaaagaatg attggtaaac tgcagacctt
    40621 gtttctgact ccacttttgg aaatactggc gcaattcttg ttcttttact tctactgcat
    40681 cgaagacctt ggcatcacgc ttaagtaatt tgactaaccg tcttttgcta tccagctttc
    40741 cttctgcaaa gattatcaac ttggttgttg gagaaggatt gtcaaggtat tcctcaaatg
    40801 acttaagctc atcatctgtc aaaaagcgtt tcttagcagt cgtgatatcc ataaaataat
    40861 ctaatatcac tattttctca tccgcaaaga aaggaaggct gaccaactcc agttccacat
    40921 ccttgtaaac tacttctttc atatcaaagt aggcaaagtt gaggtcagca gaatcatacc
    40981 caatctgttt caacatttga ctcttcatca cttcaaactg accctgatct gtccctgtaa
    41041 ataggctcag gctcggtaaa tttgataaag tcaacttctg actttcttca atggctagca
    41101 tcgactctcc tttcttcaga tttttcgatt taatttagtc aatatagcgc aatttcccac
    41161 ggaaatcttc taagctctcg tagccttttt ccaccatgat tgctttcagt tcattggtaa
    41221 tgcggtcaaa agcactgacg ccttctttgt gaagggtcgt tcccacctgc accatacttg
    41281 ctccacagag gatgtgttca aaggcatctc gaccagtcag aacgccacct gttccgataa
    41341 tttggatttg aggatttaaa cgttgataaa aggcgtgaac attggctaga gcagtcggtt
    41401 tgatgtattc tccaccaatt ccaccaaaac cattcttagg ccgaataacg acagattcgt
    41461 cttctatata gaggccgttt ccgatagagt taacgcagtt gacaaacttg agcggatatt
    41521 tgttgaaaat agctgccgct tggtcaaagt gaacaatatc aaaataaggt ggcaatttaa
    41581 ttccaagagg tttggtgaag taagcaaaca cttctgccaa aatccggtct gttgtctcaa
    41641 aatcataggc aatctgaggt ttacctggaa catttggaca ggaaagattt agctcagtca
    41701 gaccacgaaa atcactctct tggacttttt tcaaaatagt atgggtttcc tctggagaca
    41761 tgccgaccag agataagaag aaagttcggt tcgactcttt ttcctgcaaa tctaaaagat
    41821 aatccaaata atagtctaag ccattatttg gcaagcccat agagttgatg gaaccaagtg
    41881 gaacatcttg gtagcgtggc tcaggattcc cctgacggaa gtccaaggtc gctgtcttag
    41941 taacaaaggt tcctgccgct gagtttttga cctcttctaa ctcctctatc gtcatacaag
    42001 ccacacctgc tgcattcatc aagcaattgt caaactcaaa accagcaatt tgtgttttcg
    42061 ttgataccat gattctaatc ctccagattt cttttattgc taatattata ccataatttt
    42121 aattctaggt ttactagatt ctgtggttct atctatttat tcggaaaaga aagtctagta
    42181 cagaagtcat gctatggaaa cagctagtgt attggaagtt tgaacaaggc ttgggtaaca
    42241 gacttttgaa tgaagtccaa gcaacaagga gaaatctcaa ccatgagtcc attccatgac
    42301 tctgttgctc tagaaaacct tgcaatagtc aagtgataac gaataaaact ttggattgaa
    42361 atatccccat gattatctat tacgaggaga aatctcaacc tttatcattt ttagagaaat
    42421 atagtaaaat gaaacaagaa caggagaaat cgatcaggac agtcaaatcg atttctaaca
    42481 atgttttaga agttgaggtg tactattcta gtttcaatct actatacatg
//
LOCUS       DBERDK010000010        42002 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.010, whole genome shotgun sequence.
ACCESSION   DBERDK010000010 DBERDK010000000
VERSION     DBERDK010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 42002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42002
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.010"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..1090
                     /locus_tag="ACL73L_000509"
     CDS             <1..1090
                     /locus_tag="ACL73L_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit family protein"
                     /protein_id="HGS0771543.1"
                     /translation="DFILPNIILEIRRISQNTEVESITTLSEEDMDIVGFLNDHFVEK
                     NTHSVSNLLFKDKYFMRSFLVGVVNQPYFRLLESENDLRVFWNNCKSSAAILKPRNEA
                     GSNGIKKVYPNEKIDSVYFEKNYIIEEYIDIDNMITCDGYSIGSEIKRFYVHEYEELL
                     FNTLSSSGYYLVRTSSLYNDSEKIEYIEKALEECRKVLNVFSINSEITPFHFEWFLEL
                     STGKIVFCEVGKRFGGGDIPQLIEDSHGVDVIKEYWDILTNSQGLKKINFSKVISRPA
                     NISATFALYRKQGKVIEVPDEKELAWAKKVYIFIKPNTYYKVSENVVENSMLVQFNCK
                     NSCEFEKRLDELKHISQKFKYVITDSGE"
     gene            1092..2303
                     /locus_tag="ACL73L_000510"
                     /pseudo
     CDS             1092..2303
                     /locus_tag="ACL73L_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428251.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            2983..3204
                     /locus_tag="ACL73L_000511"
     CDS             2983..3204
                     /locus_tag="ACL73L_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGS0771544.1"
                     /translation="MYRRLRDLREDHDLTQKQIAKILSFTNSAYAKIERGEHTLTADV
                     LVTLSNFYDVSTDYLLELTDFPDKIRFRK"
     gene            3650..3974
                     /locus_tag="ACL73L_000512"
                     /pseudo
     CDS             3650..3974
                     /locus_tag="ACL73L_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            4102..5331
                     /locus_tag="ACL73L_000513"
     CDS             4102..5331
                     /locus_tag="ACL73L_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HGS0771545.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGISVNGA
                     YGWISVAGITIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLIMYTVSGIAYRWFSTILALVSAASVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFVGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNLLLK"
     gene            5351..8047
                     /gene="ppc"
                     /locus_tag="ACL73L_000514"
     CDS             5351..8047
                     /gene="ppc"
                     /locus_tag="ACL73L_000514"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HGS0771546.1"
                     /translation="MSLQKLENYSNKSVVQEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWYNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAKTLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATSLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDCIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITQKKSDTNTPNRYEAIMDQVVDRS
                     YDIYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYETILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     misc_binding    8130..8347
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8460..9464
                     /gene="trpX"
                     /locus_tag="ACL73L_000515"
     CDS             8460..9464
                     /gene="trpX"
                     /locus_tag="ACL73L_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HGS0771547.1"
                     /translation="MKNKRLIGIIAALAVLVAGSLIYSSMNKSEAQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATSAAQGLASATKDLPVIMAAITDPIGANLVKGLKKPGGNVTGVSDHNPAQQQ
                     IELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTSKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSTGKSVINKKIAQELGITIPESVLKEAGQVIE
                     "
     gene            9533..10399
                     /locus_tag="ACL73L_000516"
     CDS             9533..10399
                     /locus_tag="ACL73L_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS0771548.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGTKQIQDVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGRMELMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTSYLRLYSALILAVCLMIPTF
                     KQTILKGAKLSK"
     gene            10396..11154
                     /locus_tag="ACL73L_000517"
     CDS             10396..11154
                     /locus_tag="ACL73L_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0771549.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFERDFITILGGN
                     GAGKSTLFNTIAGTLSLTSGTIRILGEDLTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLLPRRLTSYKDEFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGRIIQDLNQEEKAKMKISDYYQLFE"
     gene            11244..13004
                     /gene="dnaG"
                     /locus_tag="ACL73L_000518"
     CDS             11244..13004
                     /gene="dnaG"
                     /locus_tag="ACL73L_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HGS0771550.1"
                     /translation="MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVPFIEAVQILGQRVGIEVEKPLYSEQ
                     KSASPHQALYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
                     RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIW
                     QKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKRSSGKASEIYLMEGFMDVIAAYRAGI
                     ENAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAATLKALDEIGDMPVQIVSMP
                     DNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFLEKIAPLI
                     VQEKSIAAQNSYIHILADSLASFDYTQIEQIVNESRQVQRQNRMEGISRPTPITMPVT
                     KQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLPPEVL
                     AEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEASHVG
                     DTDTALEELERLISQKRRME"
     gene            13007..14116
                     /gene="rpoD"
                     /locus_tag="ACL73L_000519"
     CDS             13007..14116
                     /gene="rpoD"
                     /locus_tag="ACL73L_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HGS0771551.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            14130..14459
                     /locus_tag="ACL73L_000520"
     CDS             14130..14459
                     /locus_tag="ACL73L_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HGS0771552.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            14574..15617
                     /gene="cpoA"
                     /locus_tag="ACL73L_000521"
     CDS             14574..15617
                     /gene="cpoA"
                     /locus_tag="ACL73L_000521"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HGS0771553.1"
                     /translation="MEKKKLRINMLSSSEKVAGQGVSGAYRELVRLLHRAAKDQLIVT
                     ENLPIEADVTHFHTIDFPYYLSTFQKKRSGRKIGYVHFLPATLEGSLKIPFFLKGIVK
                     RYVFSFYNRMEHLVVVNPMFIEDLVAAGIPREKVTYIPNFVNKEKWHPLPQEEVVRLR
                     TDLGLSDNQFIVVGAGQVQKRKGIDDFIRLAEELPQITFIWAGGFSFGGMTDGYEHYK
                     KIMENPPKNLIFPGIVSPERMRELYALADLFLLPSYNELFPMTILEAASCEAPIMLRD
                     LDLYKVILEGNYRATAGREEMKEAILEYQANPAVLKDLKEKAKNISREYSEEHLLQIW
                     LDFYEKQAALGRK"
     gene            15633..16958
                     /locus_tag="ACL73L_000522"
     CDS             15633..16958
                     /locus_tag="ACL73L_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HGS0771554.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGMLIRPSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLAAFADVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGTLYYGE
                     HDLAGAILEALIATPDMNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFKTVLYLQQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            16989..17165
                     /locus_tag="ACL73L_000523"
     CDS             16989..17165
                     /locus_tag="ACL73L_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HGS0771555.1"
                     /translation="MRSGRDKKIAGVCAGVAHYLDMDPTIVQVIWGVLTCCYGAGIVA
                     YIILWIIAPVATDY"
     gene            17224..17358
                     /locus_tag="ACL73L_000524"
     CDS             17224..17358
                     /locus_tag="ACL73L_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HGS0771556.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            17422..18732
                     /gene="obgE"
                     /locus_tag="ACL73L_000525"
     CDS             17422..18732
                     /gene="obgE"
                     /locus_tag="ACL73L_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HGS0771557.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGMTKGMHGRGAEDLRVRVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENLEDFKKKLAENYDEFEELPAIFPISGLTKQGLATLLDATA
                     ELLDKTPEFLLYDESDMEEEAYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGVDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            18742..18900
                     /locus_tag="ACL73L_000526"
     CDS             18742..18900
                     /locus_tag="ACL73L_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771558.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
     gene            20008..21267
                     /locus_tag="ACL73L_000527"
     CDS             20008..21267
                     /locus_tag="ACL73L_000527"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HGS0771559.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLL
                     GRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEMGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGASVKATDLRAGAALVIAGLMAEGKTEITN
                     IEFILRGYSDIIEKLRNLGADIRLVED"
     gene            21283..21834
                     /locus_tag="ACL73L_000528"
     CDS             21283..21834
                     /locus_tag="ACL73L_000528"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS0771560.1"
                     /translation="MNIWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALANYFMKSPLGVWAICDQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLSIITHLENEASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFNE"
     gene            21827..23104
                     /gene="spxR"
                     /locus_tag="ACL73L_000529"
     CDS             21827..23104
                     /gene="spxR"
                     /locus_tag="ACL73L_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS-HotDog domain-containing transcription
                     factor SpxR"
                     /protein_id="HGS0771561.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIERLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVL
                     RSKHDTFTVATTINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFLVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            23120..23980
                     /locus_tag="ACL73L_000530"
     CDS             23120..23980
                     /locus_tag="ACL73L_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HGS0771562.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKSGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDVTKEA
                     MYKGIEQAVIGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
     gene            24359..25152
                     /locus_tag="ACL73L_000531"
                     /pseudo
     CDS             24359..25152
                     /locus_tag="ACL73L_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            25168..25665
                     /locus_tag="ACL73L_000532"
                     /pseudo
     CDS             25168..25665
                     /locus_tag="ACL73L_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234238.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
     gene            25822..26247
                     /gene="lacA"
                     /locus_tag="ACL73L_000533"
     CDS             25822..26247
                     /gene="lacA"
                     /locus_tag="ACL73L_000533"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050044 - galactose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HGS0771563.1"
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGQDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMG"
     gene            26278..26793
                     /gene="lacB"
                     /locus_tag="ACL73L_000534"
     CDS             26278..26793
                     /gene="lacB"
                     /locus_tag="ACL73L_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HGS0771564.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPSEENKKLIAKIEHLESHNAQQTDANFFTEF
                     LEKWDRGDYHD"
     gene            26804..27733
                     /locus_tag="ACL73L_000535"
     CDS             26804..27733
                     /locus_tag="ACL73L_000535"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009024 - tagatose-6-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HGS0771565.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTVNRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGKLGEFLVEHIDNQVKKDFFSIQGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQDFLEHFKKLLESVEVVAISGSLPAGLPVDYYASLVELANQAGKPVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLDELKEVLQEPLFAGIEWI
                     IVSLGANGTFAKHGDTFYKVDIPRIQVVNPVGSGDSTVAGISSGLLHKESDAELLIKA
                     NVLGMLNAQEKMTGHVNMANYQVLYDQLIVKEV"
     gene            27735..28715
                     /gene="lacD"
                     /locus_tag="ACL73L_000536"
     CDS             27735..28715
                     /gene="lacD"
                     /locus_tag="ACL73L_000536"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HGS0771566.1"
                     /translation="MALTEQKRVRLEKLSDENGIISALAFDQRGALKRLMAQHQTEEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDEKVGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSDPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAAFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGEAAAREWLRTTGFENIDELNKVLQTTATSWKERV"
     gene            28949..29785
                     /locus_tag="ACL73L_000537"
     CDS             28949..29785
                     /locus_tag="ACL73L_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HGS0771567.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEEVIVIGKGIAFGKKKGDLIAENQ
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALTQGRYKDSNLPDISAKYPVAFQIANEAFEIYRQKLADHFPEDEIIRIAYHF
                     INAEGENEVELVESIDKRKEILRNVEEVLTDYAIQRTKKNNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEDHIKQSYPKAFEIGSKIYDVITQHTGLDLYKSERVYLVLHIQRL
                     LS"
     gene            29821..30138
                     /locus_tag="ACL73L_000538"
     CDS             29821..30138
                     /locus_tag="ACL73L_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HGS0771568.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALKAAEAGDFEKADALVE
                     EAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     GVQ"
     gene            30138..31829
                     /locus_tag="ACL73L_000539"
     CDS             30138..31829
                     /locus_tag="ACL73L_000539"
                     /EC_number="2.7.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0019197 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase complex [Evidence IEA];
                     GO_function: GO:0015155 - lactose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactose-specific PTS transporter subunit EIIC"
                     /protein_id="HGS0771569.1"
                     /translation="MNKLIAFIEKGKPFFEKLSRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAFVPNSWGFKWSDEVVAFLMKPYSYSMGILALLVAGTTAKSLTDSVNRSMEKTNQ
                     INYMSTLLAAIVGLLMLAADPIESGLATGFLGTKGLLSAFLAAFVTVAIYKVCVKNNV
                     TIRMPDEVPPNISQVFKDVIPFTLSVVSLYALDLLARYFVGSSVAESIGKFFAPLFSA
                     ADGYLGITIIFGAFAFFWFVGIHGPSIVEPAIAAITYANAEVNLNLLQQGMHADKILT
                     SGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNRAIGRASVVPTFFGVNEPILFGAPLV
                     LNPIFFIPFIFAPIANVWIFKFFIETLGMNSFTANLPWTTPAPLGLVLGTNFQVLSFI
                     LAALLIVVDVVIYYPFLKVYDEQILEEERSGKSNDELKEKVAANFNTAKADAILEKAG
                     VDAAQNTITEETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGGYGAHREMLPE
                     FNLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQEQFD"
     gene            32044..33450
                     /gene="lacG"
                     /locus_tag="ACL73L_000540"
     CDS             32044..33450
                     /gene="lacG"
                     /locus_tag="ACL73L_000540"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HGS0771570.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTGYGQVNAKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKEKGYKGEIGVVHAL
                     PTKYPLDHENPADVRAAELEDIIHNKFILDATYLGHYSEKTLAGVEAILAANGGSLDL
                     REEDFTALEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGEKGSSKYQIKGIGRR
                     VAPDYVPRTDWDWIIYPQGLYDQIMRVKKDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKQHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAHWYKKVAETQIID"
     gene            33493..33702
                     /locus_tag="ACL73L_000541"
     CDS             33493..33702
                     /locus_tag="ACL73L_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HGS0771571.1"
                     /translation="MFKDFIQSIYEKVYIINFEKCSQIPCLTSEELKSLGKWYVSTGK
                     EWICHSDDDLEEFKNLFLNFINPEE"
     gene            complement(33813..34574)
                     /locus_tag="ACL73L_000542"
     CDS             complement(33813..34574)
                     /locus_tag="ACL73L_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGS0771572.1"
                     /translation="MLKQEKLTKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELA
                     DKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTAEKQEIAHFAGSLVEERETIFIG
                     PGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
                     QNLSNLQFSKAFVSCNGIQNGALATFSEEEGEAQRIALNNSNKKYLLADHSKFNKFDF
                     YTFYNISNLDTIVSDSKLSDSILFKLSKHIKVIKP"
     gene            complement(34756..35718)
                     /gene="nrdF"
                     /locus_tag="ACL73L_000543"
     CDS             complement(34756..35718)
                     /gene="nrdF"
                     /locus_tag="ACL73L_000543"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HGS0771573.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLK
                     EWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSAED
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEAMQDDDYNYGLD"
     gene            complement(35906..38065)
                     /gene="nrdE"
                     /locus_tag="ACL73L_000544"
     CDS             complement(35906..38065)
                     /gene="nrdE"
                     /locus_tag="ACL73L_000544"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HGS0771574.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWAELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLS
                     ILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI"
     gene            complement(38146..38364)
                     /gene="nrdH"
                     /locus_tag="ACL73L_000545"
     CDS             complement(38146..38364)
                     /gene="nrdH"
                     /locus_tag="ACL73L_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HGS0771575.1"
                     /translation="MVTVYSKNNCVQCKMTKRFLDSNNVSYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPGKLKQLA"
     gene            38715..38978
                     /locus_tag="ACL73L_000546"
     CDS             38715..38978
                     /locus_tag="ACL73L_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HGS0771576.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAISETMEKEGLA"
     gene            38984..40717
                     /gene="ptsP"
                     /locus_tag="ACL73L_000547"
     CDS             38984..40717
                     /gene="ptsP"
                     /locus_tag="ACL73L_000547"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HGS0771577.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQAAEFKEAGEAYAKQKAEWALLKDAKTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVAYNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
     gene            40971..>42002
                     /locus_tag="ACL73L_000548"
     CDS             40971..>42002
                     /locus_tag="ACL73L_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HGS0771578.1"
                     /translation="MKINKKYLVGSAAALILSVCSYELGLYQARTVKENNRVSYIDGK
                     QAAQKTENLTPDEVSKKEGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISEE
                     LLMKDPNYQLKDEDIISEIKGGYVIKVDGKYYVYLKDATHADNVRTKEEINRQKQEHS
                     QHREGGTSANDGAVAFARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRGNLSNSRTYRRQNSDNTSRTNWVPSVSNPGTTNTNTSNNSN
                     TNSQASQSNDIDSLLKQLYKLPLSQRHVESDGLVFDPAQITSRTARGVAVPHGNHYHF
                     IPYEQMSELE"
BASE COUNT        13049 a         7267 c         9002 g        12684 t
ORIGIN      
        1 agattttatt ctaccaaata tcatcttaga aattagaagg ataagtcaaa atacagaagt
       61 tgagagtatt acaaccttga gtgaggaaga catggatata gtaggttttt tgaatgatca
      121 ttttgtagaa aaaaatactc actctgtgtc taatttatta tttaaagaca aatacttcat
      181 gcgttcattt ttagttggag tagtaaatca accgtatttt aggcttttag aatcagaaaa
      241 tgatttacgt gttttttgga ataattgtaa atcttctgct gcaattttaa aacctagaaa
      301 tgaagcagga agtaatggaa taaaaaaagt ttaccctaac gagaaaattg attcagtgta
      361 ttttgaaaaa aactatatta tagaggaata cattgatatc gataacatga taacatgtga
      421 tggttattca attggaagtg agattaagcg attttatgtt cacgaatatg aggaactatt
      481 gttcaatacc ttaagttcta gtggatatta tcttgtaagg actagttcgt tatataatga
      541 tagtgaaaaa attgaataca tagaaaaagc attggaagaa tgtcgcaaag ttttgaatgt
      601 tttttctatc aattctgaaa taaccccttt tcattttgaa tggttccttg aattatcaac
      661 tggtaaaata gtcttttgtg aagttggaaa aagatttgga gggggagata ttccccagtt
      721 aattgaggat agtcatggag ttgatgtaat taaagaatat tgggatattt tgactaattc
      781 acaaggattg aaaaaaataa atttttctaa agtgatttct agacctgcta atatctcagc
      841 aacgtttgct ttatatagaa agcaaggtaa ggttatcgag gtacctgatg aaaaagaatt
      901 ggcttgggca aaaaaagtgt acatatttat taaaccaaat acttactata aagtatctga
      961 aaatgtagtc gaaaattcaa tgttggtcca atttaattgc aaaaatagtt gtgaatttga
     1021 gaaaaggtta gatgaactta agcacatatc acaaaagttt aaatatgtga ttactgattc
     1081 gggagaataa aatgattaga gaagaacatc acaatattct atccattgta gctagtagga
     1141 tgatatctcg aatcggagat atcatgtttg atttcgccaa taataccttt cttgctggtc
     1201 ttaatccaac atctctatca gtggttgcaa tttatcaatc attagaaagt atgataggtg
     1261 ttctatttaa tttatttggt ggagttattg cagatagttt caagcggaaa aaaattatta
     1321 ttactacaaa tatcttatgt ggtattgctt gcataactct ttcatttata tcccaagaaa
     1381 aatggttggt ttatgcgata gtcataacaa atgtcatttt ggcttttatg agtgcttttt
     1441 cagggccatc atacaaagct tttacgaaag aaattgtaaa aaaggacagt atatcataac
     1501 ttaattcatt gctagaaaca acaagtactg tgattaaagt aacaattcct atgattgcaa
     1561 ttttcttata taagttactt ggaatacatg gtgtgttatt actagatgga ttatcgtttt
     1621 taattgctgc ctcactaatt ttcttcatta ctcctgtaaa tgaggaggtg gatacaaaag
     1681 agaatatgac gataagagga atctttgatg atttaaaaat aggatttacg tatgtatata
     1741 gtcataaaca aattctcact ataattgccc tttcagcact tgttaacttt ttcctagcag
     1801 cctataattt gttgctgcca tacagtaatc aaatgtttgg aagtatttca agtgggctat
     1861 atggaacctt tttaacagcc gaagcgattg gtggctttat tggtgcgata ttaagtggat
     1921 ttatcaataa atccttgtca agcaaacgtt taatgctctt tttagcatat tcaggtttga
     1981 tgttaatgct aacagcgccg atctattata tgttccgcaa cgtcattatt ttagccttta
     2041 ctccagcatt atttagtcta tttctttcca tttttaatat ccaatttttc tctattgttc
     2101 aaagagacgt agataatgag tttcttggca gagtatttgg aatcatcttt acggtagcta
     2161 ttctttttat gccactaggt actgtttttt tctcagtagt tttaaatcct aacaatactt
     2221 ttaatttttt tattattggt gtatctatta cgatattatc gctaatattt agcacgctat
     2281 tgaagaggta tgatagaagt tgatagatga taaaaaatag ctgatataga gaatttagag
     2341 cgcctttgga atatattttt ggaatttaat caatcacagt tgaatgtgaa gggcaatcct
     2401 ttttagaaga atacaatcaa gataccatta tgctatttct ttagttcagt taaaatattg
     2461 agaagtatta cttttattga aaaagcatca caaaggactc caattaattt ttaatgattt
     2521 ttctaatcta attttgtggt tattgacaaa taaagtctag ttaattgctt tgaaatccat
     2581 ggagtggtct atatacatct gttaatgcaa cggaaaattt tattgataag tagggggtgg
     2641 ctattattca acttttaggt tctgaaagta agagattcac attagaaata tcatgttgac
     2701 catttttcaa tttgctggtt agtagtaatc tggacatcat taatcccttc cttaacaaaa
     2761 ttatgtgact tttgttaccg gattactgct gattttttaa tagtttataa catctttaga
     2821 cggttaatag attttgaccg tctttcttaa tattgtctta cctataatag atatgcttat
     2881 gagatatgtt taataaatta attttagttc aaaatgtcca gtttggacac tttgaatgat
     2941 ttcttagttt tatatattat acttttctca ggagaaagct agatgtacag acgtttgaga
     3001 gatttgaggg aagatcacga tttgacccaa aaacaaatag ctaaaatact ttcgtttaca
     3061 aactcagctt atgctaaaat tgaacggggt gagcatacgt taacagcaga tgtattggta
     3121 actctctcaa acttttacga tgtcagtaca gactacctat tggaattgac agattttcct
     3181 gataaaattc gctttagaaa ataatctcct caatttcata gagtgtgaaa atgaggagat
     3241 tttttatttg ttctttgaaa attaaatagt ccaaaatggt actttcctca tttgtggagc
     3301 aaatttgaat ggctcgccat gataagagcg attttaaaac catcaataaa atagagcgat
     3361 actttatatg ccatgataca aatgatatac aatgatactt ctgaccgttc aggctgccat
     3421 cgtaaaagag cagcaagtga aattcttatg atgacttcat cagtcatgcc acgttaaatg
     3481 tgtgagtttg ttagagaaac gcaattaatc ctcaaaaggt tccccgaacc ttttgagttc
     3541 cacagacgca tcacgtggag tgtgtaagct tgttgctaaa agcgtaaaaa ccttggaacg
     3601 aaaggaataa tagacttcct gcgaaacaaa aatacaatac aataaaacta tggatgatga
     3661 agcaagtaaa caattaagcg atagccgttt caagagcctt gtaggtgttc agcgcacgag
     3721 atgttagctg tgtcaaaaac agcttatcaa cgcaacgtaa acgcgcaaaa ggtggacgaa
     3781 aaaccaaatt aagcctagac gatctcctca tggtaactat tcaatacatg cgagaatagc
     3841 gcacttatga acaaattgcg gctgattttg gcattcacga aagcaactta atccgtcgga
     3901 gccaatgggt tgaagtaact cttgttcaaa atggttttac gatttcaaat tctgccctaa
     3961 ttatgaaaaa cagtaaaatt cgaaggattg taaggtaaga gtttttttct ttctgaaaaa
     4021 atggtataat agcaatcaaa actagaaaat aaaacggaat ttggaacaga tttgtctgta
     4081 tcctagtaga gtggtgatgc tatgaagatt agtaagaggc acttattaaa ttattccatc
     4141 ttgattccct acttgctttt atctattttg ggcttgattg tggtctattc gaccaccagt
     4201 gctattttaa ttgaagaagg caagagcgcc ttgcagttgg ttcgaaacca aggaatcttt
     4261 tggattgtta gtttgatact gattgcctta atttataaat tgagactaga ttttttgaga
     4321 aatgagcgac taatcatttt agttatatta atagaaatgc ttttattgtt cttggctcgt
     4381 tttattggta tttccgtaaa cggggcatac ggttggattt cggttgcagg aataactatt
     4441 cagccagctg agtacttaaa aatcattatt atttggtatt tagctcaccg attctccaaa
     4501 cagcaagaag aaatagctac ttatgatttt caagttttga ctcaaaatca atggcttccc
     4561 cgtgctttta atgattggcg attcgttctc ctagttctga ttggaagttt gggaattttc
     4621 cctgatttag gaaatgcgac tattttagtc ttggtttcct tgattatgta tacagttagt
     4681 ggaatcgctt atcgctggtt ttcaaccatt ctggcgctcg tatctgccgc ttctgtcttt
     4741 gtcttgacca ctatcagcct aatcggtgtt gagacctttt caaaaattcc agtatttggc
     4801 tatgtagcca agcgctttag tgcctttttt aatccttttg ccgatcgtgc tgatgcaggt
     4861 caccagttag ctaattctta ttttgccatg gtcaatggcg gttggtttgg tctaggtctt
     4921 ggaaactcga ttgaaaaacg aggttatttg ccagaagctc atacagactt tgtcttttct
     4981 atcgtgattg aagaatttgg ctttgttggt gccagtctta ttttagctct cttgtttttc
     5041 atgattttgc ggattatctt ggtcggtatt cgagcggaga atcctttcaa tgccatggtt
     5101 gcactcggtg tcggagggat gatgttggtt caggtatttg tcaatatcgg agggatttcg
     5161 ggcttgattc catctacagg agtaaccttc cccttcttat cccagggtgg aaatagtctt
     5221 ctagtcttat cagtggcagt agcctttgtc ttaaatattg atgccagtga aaaacgcgct
     5281 aagttgtacc gagaattgga aaatcaacca atgaaccttc tgttgaagta ggataaagaa
     5341 aggatagttt atgtctcttc aaaaattaga aaattatagt aataaaagtg ttgtgcaaga
     5401 agaagtcttg attctaacag aattactgga agatattact aaaaatatgc ttgccccaga
     5461 gacctttgaa aaaataatac agttgaaaga attatcaacg caggaagatt atcaaggtct
     5521 aaaccgtcta gtgactagct tatcaaatga tgaaatggtc tatatttcac gctatttctc
     5581 tatcttgcct cttttgatta atatttcaga ggatgtggat ttagcttatg aaatcaatca
     5641 tcaaaataat attgatcagg actatttagg taaattatct acaacgatta aattggtagc
     5701 agaaaaggaa aatgccgttg agatcctaga acacttgaat gttgtccctg ttttgacagc
     5761 ccatccaaca caagtgcaac gcaaaagtat gttggattta acaaatcata ttcatagtct
     5821 tttgcgtaaa taccgtgatg ttaagttggg gttgatcaat aaagataaat ggtacaatga
     5881 tttgcgtcgt tacatcgaaa ttatcatgca gacagacatg attcgtgaga aaaaattaaa
     5941 agtgactaac gaaatcacga atgctatgga atattataac agctcctttt tgaaagctgt
     6001 acctcatttg acgacggagt ataagcgctt agcgcaagcg catggtctga atttaaaaca
     6061 ggctaaacca atcaccatgg gtatgtggat aggtggtgac cgtgatggaa atccatttgt
     6121 tacagcaaag accttgaagc agtctgcact cactcagtgt gaagtcatca tgaactacta
     6181 tgataaaaag atttaccaac tttatcgtga attttctctt tcaactagca ttgtcaacgt
     6241 cagcaagcaa gtcagagaaa tggctcgtca atccaaggat aactcgattt accgcgaaaa
     6301 agagctttac cgtcgtgcct tgtttgatat tcaatcaaaa attcaggcaa ctaaaaccta
     6361 tctgattgag gatgaagaag ttgggactcg ttatgaaacc gccaatgatt tctacaagga
     6421 tttgattgcc attcgagatt ctctactaga aaataagggt gagtccttga tttcaggtga
     6481 ttttgtggaa ttattgcagg cagtagagat atttggtttt tacttagcgt caattgatat
     6541 gcgacaagac tctagcgtct atgaagcctg tgtggcagaa ctcttgaaat cagcaggaat
     6601 tcattctcgt tatagcgagt tgagcgaaga agaaaagtgt gaccttctct tgaaagaatt
     6661 agaagaagat ccccgaattc tttctgcgac tcacgcagaa aaatcagaat tattagcaaa
     6721 agaattagct atttttaaga cggctcgtgt tttgaaagat aagttgggag atgatgtcat
     6781 ccgtcagacc atcatttcac atgcaaccag cctttctgat atgctagaat tagctattct
     6841 gttaaaagag gtaggactgg tggatacgga aagggcgcgt gttcagattg ttcccctttt
     6901 tgaaacaatt gaagacttgg atcattcaga ggaaacaatg agaaaatatc tttctcttag
     6961 ccttgccaaa aaatggattg actcacgaaa taactaccaa gaaatcatgc ttggctactc
     7021 tgacagtaat aaagatggcg gttacttgtc atcatgttgg accctctaca aggctcaaca
     7081 acaattgact gctattggag atgaatttgg cgttaaggtt accttcttcc atggtcgtgg
     7141 tggtactgtc ggtcgtggtg gtgggccaac ctatgaagcc attacatctc aaccgctcaa
     7201 gtctatcaag gattgtatcc gcttgacgga gcagggtgaa gtaattggga ataaatacgg
     7261 taacaaagac gccgcttact ataaccttga aatgctagta tcggcagcta ttaaccgtat
     7321 gattactcag aagaagagcg ataccaatac cccaaatcgt tatgaagcca ttatggatca
     7381 agtagtggac cgtagttacg atatctaccg tgatttggtc tttggtaatg agcatttcta
     7441 tgattatttc ttcgagtcaa gtccaatcaa ggctatttca agttttaata ttggttctcg
     7501 tccagccgct cgtaagacta ttactgaaat cggtggtttg cgtgccatcc cttgggtatt
     7561 ctcatggtca cagagtcgtg ttatgttccc tggatggtac ggggttggtt caagcttcaa
     7621 ggaatttatc aataaaaatc cagagaatat tgctatctta cgagatatgt accaaaattg
     7681 gcctttcttc caatcgcttc tttcaaatgt tgatatggtt ttgtcaaaat caaatatgaa
     7741 tattgctttt gaatatgcta aactttgtga agacgagcaa gttaaggcca tctatgagac
     7801 tattttaaat gaatggcaag ttactaagaa cgttatcttg gctattgaag gacatgacga
     7861 actcttagct gacaatccat atctaaaagc tagtctggat taccgtatgc cttactttaa
     7921 tattctcaac tatattcagt tagagttgat taaacgccaa cgtcgtggag aattgtccag
     7981 tgatcaagaa cgattgattc atatcaccat caacggaatt gcgacaggat tgcgtaattc
     8041 aggttgataa ttttcaagag tgaatgctaa aagtgaatat caaaaaaatt ctaatagact
     8101 attgacaagt agtttaaaaa tgatataatt taaccattca gaaaagtaat catacaaact
     8161 ttttagagag tctgtggtag ctgaaaacag ataagtggcg atgatgaaaa ttgggctgaa
     8221 tgctatttag aatttgaaat tataaaaatt cggtaagcac accttacagt gcatctcgtt
     8281 attgcgagac tgagcgatag ggaaattccc tataattgag gtggtaccgc gcatcgacgt
     8341 ccttacacaa gttttttgtg tgaggatttt tttgatggag gttagtatgg aaagaaaacg
     8401 atggcgtcgc ttgtttagat aagtgaaata tgttaaagga aataaaaagg agaaacagaa
     8461 tgaaaaataa acgtttaatt ggaattattg ctgcattagc agtcttagta gcaggaagct
     8521 tgatttattc ttcaatgaat aaatcagaag ctcagaataa taaggatgag aagaaaataa
     8581 ccaagattgg tgtgcttcaa tttgtgagcc atccatccct tgatttgatt tataaaggga
     8641 tccaagatgg acttgcagaa gaaggatata aagatgatca agttaaaatt gattttatga
     8701 actcagaagg tgaccaaagt aaggttgcga caatgagtaa acaattggtt gcaaatggga
     8761 atgaccttgt ggttggtatc gcaacatcag cagcccaagg gttggctagt gcaacaaaag
     8821 acctaccagt tatcatggcc gctattacag acccaattgg tgctaacttg gttaaaggtt
     8881 tgaaaaaacc aggtggcaac gttacagggg tatctgacca caatccagct caacaacaaa
     8941 ttgaactcat caaggctctg acaccgaatg tgaaaacaat cggagctctt tactcaagta
     9001 gcgaagacaa ttcaaaaaca caggtcgaag aatttaaggc ttatgctgaa aaagcaggtc
     9061 tgacagtgga aacatttgca gttccttcaa caaatgaaat tgcctcaact gtcactgtta
     9121 tgactagcaa ggtagatgct atttgggttc caattgataa caccattgca tcaggatttc
     9181 caacggttgt ctctagcaat caaagttcta agaaaccaat ttatcccagt gcgacagcta
     9241 tggtagaagt aggtggtttg gcatcagttg taattgacca acatgacctt ggtgtggcaa
     9301 caggtaaaat gattgtgcaa gtcttgaaag gtgcaaaacc agccgatacc ccagtcaatg
     9361 tcttttcaac tggtaagtca gtcatcaata aaaaaatagc acaagaacta ggtattacta
     9421 ttcctgagtc tgttctcaaa gaagcaggac aagtcatcga ataaataagg tagggaggag
     9481 gagactcttc ccatttttta aaaagatgaa tatagaaagg attaaataaa atatgattgt
     9541 ttccattatt tctcaaggat ttgtctgggc tattctaggt ctgggaatct ttatgacatt
     9601 taggatttta aactttccag atatgacgac agaaggttcc ttccctcttg ggggagctgt
     9661 tgctgtcact ttgataacca aaggcgtgaa cccattttta gcgacacttg ttgctgtagg
     9721 agcaggttgt ttggctggaa tggcagcagg ccttctttat acaaaaggga agatcccaac
     9781 cttgctctca gggattttgg tgatgacttc ttgtcactca atcatgctct tgattatggg
     9841 acgtgcgaat ttaggcctgc ttggaaccaa gcaaattcag gatgttttgc cttttgattc
     9901 ggatttgaat caactcttga caggtctcat ctttgtgagt attgttattg ctctcatgct
     9961 ctttttcttg gacactaaac tcggacaagc ctatattgct acaggggata atcctgatat
    10021 ggctagaagt ttcgggattc atactggacg catggagctc atgggcttgg tcttatcaaa
    10081 tggtgtgatt gcccttgcag gtgccctcat tgctcagcaa gaaggttatg ccgatgtgtc
    10141 tcgagggatc ggggttatcg ttgtggggct tgcaagtttg attattggag aagttatttt
    10201 caagagtttg agcttggcag agcgtttggt tactatcgtt gtaggttcta tcgcttatca
    10261 atttttagtg tgggcagtta tcgcacttgg ctttaataca agttaccttc gtttatacag
    10321 tgccttgatt ttagcagtct gcctcatgat tccaacattt aagcaaacaa tcttgaaagg
    10381 agccaagtta agcaaatgac agcaattgta gaattaaaaa atgcaaccaa aatcgttaaa
    10441 aatggctttg atgaagaaaa gattatttta aatgatgttt ccttagaaat ttttgaacgg
    10501 gactttatca cgattttggg cggaaatggt gctggaaaat caactctctt taacactata
    10561 gcagggacct tatcactaac tagtggaact atccgtattt taggtgaaga tctcactaag
    10621 ttttcacccg agaagcgtgc caagtacctg tctcgtgtct tccaagatcc aaagatgggg
    10681 acagctcccc gtatgacggt cgctgaaaat cttttaatcg ccaagtttcg tggtgaaaag
    10741 cgtggattgt taccacgacg cttgactagc tataaggatg aatttcaggc aaccattgaa
    10801 aaagtaggaa atggtcttga gaaacacttg aatacaccga ttgagttctt atcaggtgga
    10861 caaagacagg ctttgagtct cttgatggca accttgaagc gacctgaatt actcctgtta
    10921 gatgagcata ctgctgccct ggatccaaag actagtgttg ctttgatgga attgacagat
    10981 gaatttgtta agaaagatca gctaacagcc cttatgatta ctcatcatat ggaagatgct
    11041 ctcaaatacg gcaatcgctt gattgtcatg aaagaaggac gaattatcca agatttaaac
    11101 caagaagaaa aagcaaaaat gaaaatctct gattattatc aactctttga ataaagtagg
    11161 gaaaatgagt gaaatggttt actttttttc tgaaataaag tatactatat aaagtaaact
    11221 atgataacat ggaggtattg tgtatggttg acaaacaagt cattgaagaa atcaaaaaca
    11281 atgccaacat tgtggaagtc ataggagatg tgatttcttt acaaaaggca ggacggaact
    11341 atctagggct ctgtcctttt catggtgaaa aaacaccatc tttcaatgtt gtagaggaca
    11401 agcagtttta tcactgtttc ggttgtggtc gctcaggtga tgtctttaag ttcatcgagg
    11461 agtaccaagg ggttcccttt atagaagcag tccaaatctt aggtcagcgt gttggaattg
    11521 aggttgaaaa accgctttat agtgagcaga agtcagcctc acctcaccaa gctctttatg
    11581 atatgcacga agatgcagcc aaattttacc atgctattct catgacaacg actatgggcg
    11641 aagaggccag aaattacctt tatcagcggg ggttgacaga tgaagtgctt aaacattttt
    11701 ggattggttt agcacctcca gaacgaaact atctctatca acgtttgtct gatcagtatc
    11761 gtgaagagga tttactggat tcaggcctgt tttatctctc ggatgccaat caatttgtag
    11821 acacctttca caatcgcatt atgtttcccc tgacaaatga ccaaggaaag gtcattgcct
    11881 tctcaggtcg tatctggcaa aaaacggatt cacaaacttc taagtataaa aacagccgat
    11941 cgactgcaat ttttaacaaa agttacgaat tatatcatat ggatagggca aaaagatctt
    12001 ctggaaaagc tagtgagatt tacctgatgg aaggattcat ggatgttatt gcagcctatc
    12061 gggctggaat cgaaaatgct gtggcgtcga tgggaacagc cttgagtcga gagcatgttg
    12121 agcatctgaa aaggttaacc aagaaattgg ttcttgttta cgatggagat aaggctgggc
    12181 aagccgcgac attgaaagca ttggatgaaa ttggtgatat gcctgtgcaa atcgtcagca
    12241 tgcctgataa cttggatcct gatgagtatc tacaaaaaaa tggtccagaa gacttggcct
    12301 atctattaac gaaaactcgt attagtccga ttgagttcta cattcatcag tacaaacctg
    12361 aaaacagtga aaatctgcag gctcagattg agtttcttga aaaaatagct cccttgattg
    12421 ttcaagaaaa gtccatcgct gctcaaaaca gctatattca tattttagct gacagtctgg
    12481 cgtcctttga ttatacccag attgagcaga ttgttaatga gagtcgtcag gtgcaaaggc
    12541 agaatcgcat ggaaggaatt tccagaccga cgccaatcac catgcctgtc accaagcagt
    12601 tatcggctat tatgagggca gaagcccatc tactctatcg gatgatggaa tcccctcttg
    12661 ttttgaacga ttaccgtttg cgagaagact ttgcatttgc tacacctgaa tttcaggtct
    12721 tatatgactt gcttggccag tatggaaatc ttcctccaga agttttagca gagcagacag
    12781 aggaagttga aagagcttgg taccaagttt tagctcagga tttgcctgct gagatatcgc
    12841 cgcaggaact tagtgaagta gagatgactc gaaacaaggc tctcttgaat caggacaata
    12901 tgagaatcaa aaagaaggtg caggaagcta gccatgtagg agatacagat acagccctag
    12961 aagaattgga acgtttaatt tcccaaaaga gaagaatgga gtaataatgg caacaaaaca
    13021 aaaagaagta acaacatttg acgtacaggt agcagaattt atccgtaatc ataagcaaaa
    13081 agggacagca acagatgatg aaatcaatgc aagtctggtt attcctttta ccttggacgc
    13141 tgatgggatt gaagatctct tgcaacggat tcaggatgca ggcatttcta tcacagataa
    13201 cgaaggaaat ccaagtgcgc gtgttctcag caatgaagaa gaaccagaac tcagcgatga
    13261 ggacttgatt gggtcaactt ctgctaaggt caatgaccct gtccgtatgt acttgaaaga
    13321 aataggggtc gttcctctct tgaccaatga agaggagaaa gagttggcac tggctgttga
    13381 agctggtgat atcgaagcca aacaacgtct tgcggaagcc aatcttcgtt tggttgtttc
    13441 cattgccaaa cgctatgtcg gtcgtggtat gcagttcctt gacttgattc aagaaggaaa
    13501 tatgggcttg atgaaggcgg ttgacaagtt tgactattct aaagggttca agttttcaac
    13561 ttatgcaact tggtggattc gtcaggctat cactcgtgct attgcggacc aagctcgtac
    13621 catccgtatc ccagttcaca tggttgaaac tatcaataaa ttggttcgtg aacagcggaa
    13681 tctccttcaa gaattggggc aagatccgac accagaacag attgctgaac gaatggatat
    13741 gacacctgat aaggttcgtg aaatcttgaa gattgcccaa gaaccagtat ctcttgaaac
    13801 tcctatcggt gaagaggacg atagccacct tggagacttt atcgaagatg aagtgattga
    13861 aaatccagtg gattatacga ctcgtatcgt cttgcgtgag caattggatg aaatcttaga
    13921 tactcttaca gaccgtgaag aaaatgttct gcgtctacgt tttggactag atgatggaaa
    13981 aatgcgcaca cttgaagatg tggggaaagt ctttaacgta actcgtgagc gtatccgtca
    14041 gattgaagca aaggctttga gaaaactacg ccaaccaagt cgtagcaaac cgcttcgtga
    14101 ttttattgaa gactaagagt gaggaaaata tggcttatac agaagagcaa attgaaaaca
    14161 tcaaaacacg gattttaaca gccttggaag aagtcatcga ccctgagttg ggaatcgata
    14221 ttgtcaatct tggtttgatc tatgagattc gttttgacgg tgacacaggg caaacagaga
    14281 ttgacatgac tttgacaact atgggttgtc ccttagcaga ccttttgaca gaccagattt
    14341 atgatgctat gatagaggtt ccagaagtaa cggatactga ggttaaatta gtctggtatc
    14401 cagcctggac agttgaaaaa atgagtcgct atgcccgcat tgccctaggc attaagtaaa
    14461 catataaaag catgtgagag actgttggaa aagcgaggaa atttcccctc ttttcctcta
    14521 gtctctcctt tcttttgctg attttattca aagaaaatga tataatagta gttatggaga
    14581 aaaagaaatt acgcatcaat atgttgagtt caagtgagaa agtagcagga cagggagttt
    14641 caggtgctta ccgtgaatta gttcgtcttc ttcaccgtgc tgccaaggac caattgattg
    14701 ttacagaaaa tcttccaatc gaggcagatg tgactcactt tcatacgatt gattttccct
    14761 attatttatc aaccttccaa aagaaacgct cagggagaaa gattggctat gtgcatttct
    14821 tgccagctac acttgaggga agtttgaaaa ttccattttt cttaaaggga attgtgaaac
    14881 gctatgtatt ttctttttac aaccggatgg agcacttggt tgtggtcaat cctatgttta
    14941 ttgaggattt ggtagcagct ggtattccac gtgaaaaagt gacctatatt cctaactttg
    15001 tcaacaagga aaaatggcat cctctaccac aagaagaggt agtcagactg cgcacagatc
    15061 ttggtcttag tgacaatcag tttatcgtag taggtgctgg gcaagttcag aaacgtaaag
    15121 ggattgatga ctttatccgt ctggctgagg aattgcctca gattaccttt atctgggctg
    15181 gtggcttctc ttttggtggt atgacagatg gttatgaaca ctataagaaa attatggaaa
    15241 atccccctaa aaatttgatt tttccaggca ttgtatcgcc agagcggatg cgcgaattgt
    15301 atgctctagc ggatcttttc ttgttgccta gttacaatga gctctttcct atgactattt
    15361 tagaagctgc gagttgtgag gctcctatta tgttgcgtga tttagatctc tataaggtga
    15421 ttttggaggg aaattatcgg gcgacagcgg gtagagaaga gatgaaagag gctattttgg
    15481 aatatcaagc aaatcctgct gtcttaaaag atctcaaaga aaaggctaag aatatttcca
    15541 gagagtattc tgaagagcat ctgttacaaa tctggttgga cttttatgag aaacaagccg
    15601 ctttagggag aaagtaaaaa gtgaggtaat ctatgcgaat tggtttattt acagatacct
    15661 attttcctca ggtttctggt gttgcgacca gtattcgaac cttgaaaaca gaacttgaaa
    15721 agcagggaca tgctgttttt atctttacga cgacagataa ggatgtcaat cgctacgaag
    15781 attggcaaat tatccgcatt ccaagtgttc ctttctttgc ttttaaggat cgtcgctttg
    15841 cctaccgagg ttttagcaag gcacttgaaa ttgctaaaca gtatcagcta gatattatcc
    15901 atactcagac agaattttct cttggcctgt tggggatttg gattgcgcgt gaattgaaaa
    15961 ttccagtcat ccatacctat cacacccagt atgaagacta tgtccattat attgctaagg
    16021 ggatgttgat ccggccgagt atggtcaagt atctggttag aggtttcctg catgatgtgg
    16081 atggggttat ttgccctagt gagattgtcc gtgacttgct atctgattat aaggtcaagg
    16141 ttgaaaaacg ggtcattcct actgggattg aattagccaa gtttgagcgt ccggaaatca
    16201 agcaggaaaa tttgaaagaa ctgcgtagta aactagggat tcaagatggt gaaaagacgt
    16261 tgcttagtct ttcgagaatc tcctatgaaa aaaatattca agcagtttta gcagcctttg
    16321 ctgatgttct gaaagaggaa gacaaggtta aactggtagt agctggggat ggcccttatc
    16381 tgaatgacct caaagagcaa gcccagaacc tagagattca agactcagtc atctttacag
    16441 ggatgattgc tcctagtgag acggctcttt actataaagc ggcggatttc ttcatttcgg
    16501 catcgacaag cgaaacgcaa ggtttgacct acttggaaag cttagccagt ggaacacctg
    16561 tcattgctca cggaaatcct tatttgaaca acctcatcag tgataaaatg tttggaacct
    16621 tgtactatgg agaacatgat ttggctggtg ctattttgga agccctgatt gcaacaccag
    16681 acatgaacga gcatacctta tcagagaaat tgtatgagat ttcagctgag aactttggga
    16741 aacgagtgca tgagttttat ctggatgcca ttatttcaaa taacttccag aaagatttgg
    16801 ctaaagatga tacggtcagt cagcgtatct ttaagacagt tttgtatctt cagcaacagg
    16861 tggttgctgt acctgtaaaa ggatctagac gcatgttgaa ggcttcaaaa acacagttga
    16921 tcagtatgag agactattgg aaagaccatg aagaatagaa agaggaacag ctatgaaaaa
    16981 aacaattaat gagaagcggt cgtgataaaa agattgcggg tgtttgtgct ggggtggccc
    17041 attatctgga tatggatccg actatcgttc aagtcatttg gggtgttctt acttgctgtt
    17101 acggagctgg aattgtagct tacattattt tatggattat cgcaccagta gcaactgact
    17161 attgaaaact agctcaattc atgctaagat aatagaaaat agaatgtaac gaaggagaga
    17221 aaaatggcat ttggagataa tggaaatcgt aaaaaaacta tgtttgagaa aataaccttg
    17281 tttatcgtga ttatcatgct agtagcaagt ttattgggaa tttttgcaac tgcaattggt
    17341 gccctcagta atctataaaa tagattcaag aaaatttagt gactgggatt tcccagccct
    17401 tttttaaagt gagaagaaat aatgagtatg tttttagata cagctaagat taaggtcaag
    17461 gctggtaatg gtggcgatgg tatggttgcc tttcgtcgtg aaaaatatgt ccctaatgga
    17521 ggcccttggg gtggtgatgg tggtcgtgga ggcaatgtgg tcttcgttgt agacgaagga
    17581 ctacgtacct tgatggattt ccgctacaat cgtcatttca aggctgattc tggtgaaaaa
    17641 gggatgacca aagggatgca tggtcgtggt gctgaggacc ttagagttcg agtaccacaa
    17701 ggtacgactg ttcgtgatgc ggagactggc aaggttttaa cagatttgat tgaacatggg
    17761 caagaattta tcgttgccca cggtggtcgt ggtggacgtg gaaatattcg tttcgcgaca
    17821 ccaaaaaatc ctgcaccgga aatctctgaa aatggagaac caggtcagga acgtgagtta
    17881 caattggaac taaaaatctt ggcagatgtc ggtttagtag gattcccatc tgtagggaag
    17941 tcaacacttt taagtgttat tacctcagct aagcctaaaa ttggtgccta ccactttacc
    18001 actattgtac caaatttagg tatggttcgc acccaatcag gtgaatcctt tgcagtagcc
    18061 gacttgccag gtttgattga aggggctagt caaggtgttg gtttgggaac tcagttcctc
    18121 cgtcacatcg agcgtacacg tgttatcctt cacatcattg atatgtcagc tagcgagggc
    18181 cgtgatccat atgaggacta cctagctatc aataaagagc tggagtctta caatcttcgc
    18241 ctcatggagc gtccacagat tattgtagct aataagatgg acatgcctga gagtcaggaa
    18301 aatcttgaag actttaagaa aaaattggct gaaaattatg atgaatttga agagttacca
    18361 gctatcttcc caatttctgg attgaccaag caaggtctgg caacactttt agatgctaca
    18421 gctgaattgt tagacaagac accagaattt ttgctctacg acgagtccga tatggaagaa
    18481 gaagcttact atggatttga cgaagaagaa aaagcctttg aaattagtcg tgatgacgat
    18541 gcgacatggg tactttctgg tgaaaaactc atgaaactct ttaatatgac caactttgat
    18601 cgtgatgaat ctgtcatgaa atttgcccgt cagcttcgtg gtatgggggt tgatgaagcc
    18661 cttcgtgcgc gtggagctaa agatggggat ttggtccgca ttggtaaatt tgagtttgaa
    18721 tttgtagact aggagactgg tatgggagat aaaccgatat ctttccgaga tgcggatggt
    18781 aattttgttt ccgccgcaga cgtttggaat gaaaagaaat tggaagaact atttaatcgt
    18841 ctcaatccaa atcgtgcctt gagattggca cgaactaaaa aggaaaatcc atctcagtaa
    18901 agaagctaaa aaatcccgtg cctcatcaga cacgggattt tgtggtacga caggcatgta
    18961 tagcaaactg aatctggaat agcacagcat atcttctaaa atatagtaaa atgaaatgag
    19021 aacaggacaa atcgatcagg acagtaaaat cgatttctaa caatgtttta gaagcagaga
    19081 tgtactattc tagtttcaat caactatatt gttataaatt gatttgaatt tcaaaattaa
    19141 attgtttgat tcttatttca atttgttata gtatatctga tgtcaaagtt ctcggcgagt
    19201 caaatagcga ttcccaagcc tgactatcgt gaggtagcgg attaaaatgg tctggggata
    19261 gaccgtttta agtctgacgc tggaaataag aattgtcaga agaagggata gcgaaatcgt
    19321 ggctctacga acaggaacgt gataataagg cgtatatagc ggataagagg gcctcaaact
    19381 ctaaagtcca aaaaggtagt cgtaacctat atgcgtaaat cacgagagta attgaattcg
    19441 tactaagatt ttctattttc actgtaacct tttaacgtcc tcgtatcttg tatacacgag
    19501 gaaagatgta cgacttatcc cgtgaggtct atcactataa agagaaaacg acagatagaa
    19561 gtgatcctga gtcacggtta tctttaaaat agattgtctg ataggacggt atgtataaaa
    19621 cgcttctgtg aactgagaga agggggagaa gttcttgcta aaatttagtt gaacagccgt
    19681 attccgatat ttagataagg gatctagtct tagctcctac tcagttttag gggataaaaa
    19741 aggggcaata gcgattcgag aaagattata ctcttcgaaa atctcttcaa atcacgtcaa
    19801 tatcgccttg ccgtatgtgt aggatactga ctacgtcagt tctatctaca acctcaaaac
    19861 agtgttttga gcaacctgcg gctagtttcc tagtttgatc tttgattttc atttaaaatt
    19921 gaaaaggatg gaaaaaagga taaatttatg atatacttta ttttgaagac cttattagaa
    19981 atcttgaaag agtattgaaa acttagaatg agaaaaattg ttatcaatgg tggattacca
    20041 ctgcaaggtg aaatcactat tagtggtgct aaaaatagtg tcgttgcctt aattccagct
    20101 attatcttgg ctgatgatgt ggtgactttg gattgcgttc cagacatttc ggatgtagcc
    20161 agtcttgtcg aaatcatgga attgatggga gctactgtta agcgttatga cgatgtattg
    20221 gagattgacc caagaggtgt tcaaaatatt ccaatgcctt atggtaaaat taacagtctt
    20281 cgtgcatctt actattttta tgggagcctc ttaggccgtt ttggtgaagc gacagttggt
    20341 ctaccgggag gatgtgatct tggtcctcgt ccgattgact tacaccttaa ggcgtttgaa
    20401 gctatgggtg cgactgctag ctacgaggga gataacatga agttatctgc taaagataca
    20461 ggacttcatg gtgcaagtat ttacatggat acggttagtg tgggagcaac gattaatacg
    20521 atgattgctg cggttaaagc aaatggtcgt actattattg aaaatgcagc ccgtgaacct
    20581 gagattattg atgtagctac tctcttgaat aatatgggtg cccatatccg tggggcagga
    20641 actaatatca tcattattga tggtgttgaa agattacatg ggacacgtca tcaggtgatt
    20701 ccagaccgca ttgaagctgg aacatatata tctttagctg ctgcagttgg taaaggaatt
    20761 cgtataaata atgttcttta cgaacacctg gaagggttta ttgctaagtt ggaagaaatg
    20821 ggagtgagaa tgactgtatc tgaagacagc atttttgtcg aggaacagtc taatttgaaa
    20881 gcaatcaata ttaagacagc tccttaccca ggctttgcaa ctgatttgca acaaccgctt
    20941 acccctcttt tactaagagc gaatggtcgt ggtacaattg tcgatacgat ttacgaaaaa
    21001 cgtgtaaatc atgtttttga actagcaaag atggatgcgg atatttcgac aacaaatggt
    21061 catattttgt acacgggtgg acgtgattta cgtggggcca gtgttaaagc gaccgactta
    21121 agagctgggg ctgcactagt cattgctggg cttatggctg aaggtaaaac tgaaattacc
    21181 aatatcgagt ttatcttacg tggttattct gatattatcg aaaaattacg taatttagga
    21241 gcggatatta gacttgttga ggattaaacc gtagaggtgt ttatgaatat ttggaccaaa
    21301 ttagcaatgt tttctttttt tgaaacggat cgcttgtatt tgcgtccttt cttttttagt
    21361 gatagtcagg acttccgcga gatagcttca aatccagaaa atcttcaatt tattttccca
    21421 acgcaggcaa gtctggaaga aagtcaatat gcactggcca attactttat gaagtcccct
    21481 ttgggagtgt gggcaatttg tgaccagaaa aatcaacaaa tgattggttc tattaaattt
    21541 gagaagttag atgaaatcaa aaaagaagct gagcttggct attttttgag aaaagatgct
    21601 tggtcgcaag gatttatgac agaggttgtt agaaaaattt gtcagctttc ttttgaggaa
    21661 tttggcttaa aacaattatc tatcattacc caccttgaaa atgaagctag ccaaagagtt
    21721 gctcttaagt ctggatttag tttgttccgt cagtttaagg gaagtgatcg ttacacaaga
    21781 aaaatgcggg attatcttga atttcggtat gtaaaaggag agttcaatga gtaagcatca
    21841 ggaaattcta agctatttgg aggaattacc agtaggtaaa agggtcagtg ttcgtagcat
    21901 ttcgaatcat ctaggagtta gtgatggaac agcctatcgg gctattaaag aagctgaaaa
    21961 ccgtggaatt gtggagaccc gtcctagaag tggaacaatt cgtgttaaat cccagaaagt
    22021 tgctatagag agattaacgt ttgctgaaat tgcagaagtg acttcttctg aggttctggc
    22081 tgggcaagaa ggtttagaga gagaatttag caagttttca attggtgcca tgactgaaca
    22141 aaatatcttg tcttaccttc atgatggggg gctcttgatt gtcggagacc gaacccgtat
    22201 tcagttgcta gccttggaaa atgaaaatgc agttctggtt acagggggat ttcaggttca
    22261 tgatgatgtg cttaaactgg ccaatcaaaa agggattcct gttctaagaa gtaagcatga
    22321 tacctttacc gtcgcgacca cgatcaataa agccttgtca aatgtccaaa tcaagactga
    22381 tattctgaca gttgagaaac tttatcgccc tagtcatgag tatggttttc tgagagagac
    22441 agatacagtt aaagattatt tggacttggt tcgtaagaat cgtagcagcc gtttccctgt
    22501 tatcaatcaa catcaggtcg ttgttggcgt tgtaaccatg agagacgctg gtgataaatc
    22561 accaagcacg acaattgata aggttatgtc tcgtagtcta tttttggttg gattatcgac
    22621 aaatattgcc aatgtgagtc aacggatgat cgcagaagac tttgaaatgg taccagttgt
    22681 tcgaagcaat caaactttgc ttggcgttgt gacgcgacga gatgtcatgg agaagatgag
    22741 ccgttctcaa gtttcggctc taccaacttt ttctgagcag attggacaaa agctctctta
    22801 tcaccatgat gaagtagtca ttacagtgga accctttatg ctagaaaaaa atggagtttt
    22861 ggctaatggt gtattggcag aaattctgac ccacatgacc caagatttag ttgttaatag
    22921 tggtcgcaat ctcattatcg agcagatgct gatctacttt ttgcaggctg ttcagataga
    22981 tgatatattg cgcattcagg cacggattat tcatcatacg agacggtcag ctataattga
    23041 ttacgatatt tatcatggtc accagattgt ttcaaaagca aatgtgactg ttaaaattaa
    23101 ttagaaacta ggagaaaaga tgataacatt aaaatcagct cgtgaaatcg aagctatgga
    23161 caaggctggt gattttctag caagtattca tataggctta cgtgatttga ttaagtcagg
    23221 cgtagatatg tgggaagttg aagaatatgt ccgccgtcgt tgtaaagaag aaaatttcct
    23281 tccacttcag attggggttg acggtgccat gatggactat ccttatgcta cctgttgctc
    23341 tcttaacgat gaagtggctc acgctttccc tcgtcattat atcttgaaag atggtgattt
    23401 gctcaaagtt gatatggttt tgggaggtcc cattgctaaa tctgacctga atgtctcaaa
    23461 attaaacttc aacaatgttg aacaaatgaa aaaatacact cagagctatt ctggtggttt
    23521 agcagactca tgttgggctt atgctgttgg tacaccgtcc gaagaagtca aaaacttgat
    23581 ggatgtaacc aaagaagcta tgtacaaggg tattgagcaa gctgttattg gaaatcgtat
    23641 cggtgatatc ggtgcggcta ttcaagaata cgctgaaagt cgtggttacg gtgtagtgcg
    23701 tgatttggtt ggtcatggtg ttggcccaac tatgcacgaa gaaccaatgg ttcctaacta
    23761 tggtattgca ggtcgtggac tccgtcttcg tgaaggaatg gtcttaacca ttgaaccaat
    23821 gatcaataca ggcgattggg aaattgatac agatatgaaa actggttggg cgcataagac
    23881 cattgacggt ggattgtcat gtcagtatga acaccaattt gtcattacga aagatggacc
    23941 tgttatcttg actagccaag gtgaagaagg aacttattaa taaaaagtga aaagactact
    24001 ggaagtttat tatgaaaaaa tccagtagat cttttcataa taaaacgcat tgtatcaagt
    24061 gttaggggct gatatcatgc gtttttctgc ttttaagatt ttttccaact ctgtttgtaa
    24121 gcgcatcata acaaagggtc taggattcag ggctctcctc ctatatacta ttagtaaagt
    24181 aaaactaagg gaggatattt tagtgtcgca gtctattgtt cctgtagaga ttccacaata
    24241 ttgtcgtttt gattctaaaa agagaaatgg aattctgttt aatgttcgta ttgccaatct
    24301 taaatttacc ttttttagat tagactccct gcgaaacaaa acatggtata gtagttctat
    24361 gaatgatgaa gcaagtaaac aactaactga tgcacgattt aagcgttttg ttggtgttca
    24421 gcgcacgact tttgaagaga tgttagctgt attaaaaaca gcttatcaac ttaaacacgc
    24481 aaaaggtgga cgaaaaccta aattaagcct agaagacctt cttatggcta ctcttcaata
    24541 tgtgcgagaa tatcgaactt atgaacaaat tgcggctgat tttggtatcc acgaaagcaa
    24601 ctttatccgt cggagccaat gggttgaagt aactcttgtt caaagtggtg ttacgatttc
    24661 aagaactcct ctcagctctg aggacacggt aatgattgat gcgacggaag tacaaatcaa
    24721 tcgccctaaa aaaagaatta gcgaatgatt ctggtaaaaa gaaatgccac gctatgaagg
    24781 ctcaagcgat tgtcacaagt caagggagaa ttgtttcttt ggatatcgct gtgaactatt
    24841 tgttatgata tgaagttgtt caaaatgagt cgcagaaata tcggacaagc tggtaaaatc
    24901 ttggctgaca gtggttatca agggctcatg aagatatatc ctcaagcaca aactccacgt
    24961 aaatccagca aactcaagcc gctaacagct gaagataaag cctgtaacca tgcgctatcc
    25021 aaggagagaa gcaaggttga gaacatcttt gccaaagtaa aaacgtttaa aatgttttca
    25081 acaacctatc gaaatcatcg taaacgcttc ggattacgaa tgaatttgat tgctggtatt
    25141 atcggattct agttttgcag gaagtctaat aaagaaacta gcaaggtcag gccttacttt
    25201 attggtggac atccaggatt taattgtctt ctacttgatg atgaagtcta tgaagattat
    25261 tatctagagt ttgagaaaga agagatttgc tctgttccac gtcctttccc agaaacaggt
    25321 atgttggatt tccaagatag aagtccatgg ctagagggac aaaaagaaat agatcttagt
    25381 tatgatcttt tcagcacaga tgcagtgact ttggatgaat tgcaatctcg aacaattgcc
    25441 cttcgttctc ttaaacatga taagggattg aaagtgcatt ttgcagaatt tccaaacctc
    25501 atcatctggt caactttgaa caagggacct ttcattacct ttgaaccatg gtctggcttg
    25561 tcaacattcc ttgaagaagg agatcattta gaagataaga agaatgtttg tctcttagaa
    25621 gccaatcagg ttgaagaatt agggtttgag atagaagttt tataaaaaag taaaaaataa
    25681 ataacaaaaa caaacacaaa ctgttgactt cttcaaacaa tagtagtata ttatgtttgt
    25741 gaggactaat tgactgtttt ttaaagacag ccaatacact gctggaatcc agcatagaaa
    25801 aaataaaaag gagtatacaa tatgtctatt gttatcggtg cagatgctgc aggtttgaga
    25861 ttgaaagaag ttgtgaaaga ctttttagaa aaagaaaact tccaccttgt ggatgttaca
    25921 gctgaaggtc aagactttgt tgatgtgact ctcgctgttg ctgcagaagt aaacaaagaa
    25981 gaacaaaacc ttggtatcgt gattgatgct tatggagctg gtccatttat ggttgcaact
    26041 aagatcaaag gaatggttgc tgcagaagta tctgacgaac gttcagctta tatgactcgt
    26101 ggccacaaca actcacgtat gatcactatg ggggcacaac ttgttggtga tgaattggct
    26161 aaaaacatcg ctaaaggatt tgttaatggt aaatacgacg gtggtcgtca ccaaatccgg
    26221 gttgacatgt tgaacaaaat gggctaatta gattatagta agaaaggtaa gttaaaaatg
    26281 agaattgcaa ttggatgtga ccacatcgta actgatgaaa aaatggcggt ttcagaattt
    26341 ttgaaatcaa aaggatatga agtcattgac tttggtacat atgaccatac acggactcac
    26401 tacccaatct ttggtaaaaa agtaggggaa gcagtaacta gtggtcaagc tgatctcggg
    26461 gtatgtatct gtggtactgg tgttggtatc aacaacgctg taaataaagt tccaggtgtt
    26521 cgttctgcct tggttcgtga tatgacaaca gccctttatg ctaaagaaca attgaacgcc
    26581 aacgttatcg gttttggtgg taagattact ggtgaattgc ttatgtgtga tatcatcgaa
    26641 gctttcattc atgctgaata caaaccatct gaagaaaaca aaaaattgat tgcgaaaatt
    26701 gaacaccttg aaagtcacaa tgctcaacaa acagacgcaa acttcttcac tgaattcctt
    26761 gaaaaatggg atcgcggaga ctaccacgac taagaggtaa actatgattt taacagttac
    26821 aatgaaccca tccattgata tttcctatcc cttggatgag ttgaagattg atactgtcaa
    26881 tcgtgtggtg gatgtaacca aaacggctgg tggtaaggga ctcaatgtta cccgagtact
    26941 ttcagaattt ggcgattctg ttcttgctac tggtttagtg ggtggcaaac ttggtgagtt
    27001 tttggttgaa catatcgata atcaagtaaa gaaagatttc ttctcaattc agggagaaac
    27061 tcgtaactgt atcgctattc tccacggaga caaccaaaca gaagttcttg aaaaaggtcc
    27121 tgaagtattg gaacaggaag gtcaagactt tttggaacat ttcaaaaaac tcttggagtc
    27181 agttgaagta gtggccatct caggtagtct gccagctggc cttccagttg attactatgc
    27241 gagcttggta gaacttgcta atcaagctgg caagcctgta gtcttggact gctcaggtgc
    27301 agcacttcag gctgttcttg aatcacccca taaaccaaca gtcatcaaac caaataatga
    27361 agaattgtct cagcttcttg gaagagaagt ttctgaggat ttggatgaat taaaagaagt
    27421 acttcaagaa cctttgtttg cagggattga atggattatc gtttcacttg gtgccaacgg
    27481 tacttttgcc aaacatggtg acactttcta caaggtagat attcctagaa ttcaggtggt
    27541 aaatcctgtt ggatctggag actctactgt ggcaggaatt tcttcaggac ttcttcacaa
    27601 agaatcggat gcagaattac tcatcaaggc aaatgtcctt ggtatgctca atgctcaaga
    27661 aaaaatgact ggtcatgtca acatggccaa ctatcaagtt ctatatgatc aattaatagt
    27721 aaaagaggta taaaatggct ttaacagaac aaaaacgtgt acgcttagaa aaactttctg
    27781 atgaaaatgg tatcatctca gctcttgcat ttgaccaacg tggtgctttg aaacgcctca
    27841 tggctcaaca ccaaacagaa gaaccaactg tggcccaaat ggaagaactt aaagtcttgg
    27901 tagcagatga attgactaaa tacgcttcat ctatgcttct tgaccctgag tatggacttc
    27961 cagcaactaa agctcttgat gaaaaagttg gtcttctcct tgcttatgaa aaaacaggtt
    28021 atgacacaac aagcacaaaa cgcttgccag actgcttgga tgtctggtct gcaaaacgta
    28081 ttaaagaaga aggtgcagat gcagttaaat tcttgcttta ctatgatgta gatagctcag
    28141 acgaactcaa tcaagaaaaa caagcctaca tcgaacgcat cggttctgag tgtgtggctg
    28201 aagatatccc attcttcctt gaaatccttg cttacgatga aaaaattgcg gatgcaggtt
    28261 ctgtagaata cgcgaaagta aaaccacaca aagttatcgg cgctatgaaa gtcttttcag
    28321 acccacgctt taacattgat gtcttgaaag ttgaagttcc tgttaacatt aaatatgttg
    28381 aaggcttcgc tgaaggtgaa gtagtttata cacgtgaaga agcagcagcc ttcttcaaag
    28441 cgcaagatga agcaacgaac ttgccataca tctacttgag tgctggtgta tcagctaaac
    28501 tcttccaaga tactcttgta tttgctcatg aatcaggtgc aaactttaac ggagttcttt
    28561 gtggccgtgc tacatgggca ggatcagttg aagcttacat caaagatggt gaagcagcag
    28621 ctcgcgaatg gcttcgcaca actggatttg aaaacattga tgagctcaat aaagttcttc
    28681 aaacaacagc gacttcatgg aaagaacgtg tgtaagaaag tcctcctagt ttaggaacat
    28741 gaatctaaaa aaatttaaaa aaagttgtat gtaaaggttt acaaaataac ttacttgtgc
    28801 tatacttaaa tcacaagtta atacaaggtg agtgttacta agtaatatta ggcatgatca
    28861 caggtgaatt agaaatcagc tgattttcta gttcatttgt ggtcattttt tgtacttata
    28921 tacctttaag atataaaagg aggttgacat gtatcgaatt ctaaatccaa tgaatcacaa
    28981 tgtctcgctt gtcagaaatg ataagggaga agaggtgatt gtaattggta agggaattgc
    29041 attcggaaag aagaaggggg atttgattgc tgaaaatcag gttgagaaaa tctttcggat
    29101 gaagaccgaa gagtccagag aaaactttat ggctcttctc aaagatgttc cgcttgattt
    29161 tatcacagtg acctatgaaa tcattgataa gctatcaaag aaatatcatt atccgattca
    29221 agagtatctc tatgtaacct tgacagatca tatttactgt tcttatcaag ctctaactca
    29281 aggaaggtac aaggatagta atctgccaga tatttccgct aagtatcctg tcgcttttca
    29341 aatcgcaaat gaagcttttg aaatttaccg tcagaagcta gcagatcatt ttcctgagga
    29401 cgaaattatt cggattgctt atcatttcat taatgctgaa ggtgaaaatg aagtggaact
    29461 tgtggagtcg attgataaga ggaaagaaat tctcaggaat gttgaagaag ttttaacgga
    29521 ctatgcaatt caacgaacta aaaagaataa ccatttctat gatcgcttta tgatccattt
    29581 gaattatttc ttggattatt tagacagatc tagagatgat aaccaatcac ttctggatat
    29641 ggaagatcat attaaacaat cctatccaaa agccttcgag attggttcca agatctatga
    29701 tgtgattacg caacatacgg gtcttgattt gtataaaagt gaacgagttt atctagttct
    29761 acatatccaa cgtttattgt cataaaaatt tatttaaaac tatataagga gaattctatc
    29821 atgaatagag aagaagtaac attgttaggt tttgaaatcg tagcctatgc tggcgatgct
    29881 cgttcaaaac tattggaagc cttgaaggct gctgaagctg gtgattttga aaaagcggac
    29941 gctctggtag aggaagctgg tagctgtatt gcagaggctc accacgcgca aacaagtcta
    30001 ttgactaagg aagcttcagg tgaggacttg gcttatagtg taaccatgat gcatggccaa
    30061 gaccacttaa tgacaactat cttgttaaaa gatttgatgc atcatttaat tgaactctac
    30121 aagagaggag ttcaataatg aataaactaa ttgcatttat cgagaaagga aagcctttct
    30181 ttgaaaaact atctcgtaat atctatcttc gtgctattcg tgatggtttc attgcaggta
    30241 tgcctgttat tctcttctca agtatcttta tcttgattgc ctttgtacca aactcatggg
    30301 gctttaaatg gtctgatgaa gttgtagcct ttctgatgaa accttatagc tattctatgg
    30361 gtattctggc tctcttggta gctggtacaa cagctaagtc attgactgac tcagtaaacc
    30421 ggagcatgga aaaaaccaat caaatcaact atatgtcaac attgttggca gcaattgttg
    30481 gtttgttgat gttggcagct gatcctatcg aaagtggtct agctactgga ttcttgggga
    30541 caaaaggttt gctttcagcc ttccttgctg cctttgttac tgtagccatc tataaggttt
    30601 gtgttaagaa caacgtcact attcgtatgc ctgacgaagt tccaccaaat atctcacaag
    30661 tctttaaaga tgtgattcca ttcactctat ctgttgtttc tctttatgct cttgacttat
    30721 tagcacgtta ttttgttggt tctagtgtgg cagaatcaat cggtaaattc ttcgcaccac
    30781 tcttctcagc agcagacgga taccttggta ttaccattat ctttggtgcc tttgccttct
    30841 tctggtttgt tgggattcat ggtccatcta tcgttgaacc agctatcgca gctattacct
    30901 atgccaatgc cgaagttaac ttgaaccttc tccaacaagg gatgcatgca gacaagattc
    30961 ttacttctgg tacacaaatg tttatcgtta ccatgggtgg tacaggtgcg acattggtcg
    31021 ttccatttat gttcatgtgg ttgacaaaat cgaaacgtaa ccgtgcaatc ggacgtgctt
    31081 cagtagttcc taccttcttc ggtgtaaatg aaccaatctt gtttggtgca cctcttgttt
    31141 tgaatccaat cttcttcatt ccatttatct ttgctccaat tgcaaacgta tggattttca
    31201 aattctttat tgaaactctt ggaatgaact cattcactgc taatctacca tggacaactc
    31261 cagctccact aggtctagtt cttggaacta acttccaagt gctatcattc attcttgctg
    31321 cccttctaat tgtggttgac gtagttattt attacccatt ccttaaagtc tatgatgaac
    31381 aaattcttga agaagaacgt tcaggtaagt ctaatgatga attgaaagaa aaagttgctg
    31441 caaacttcaa cactgcaaaa gcggatgcta ttcttgaaaa agcgggtgtc gatgcagcac
    31501 aaaataccat cactgaagaa acaaatgtcc tcgttctctg tgctggtgga ggtacaagtg
    31561 gactccttgc aaatgctttg aataaggcag cagcagaata caatgtccct gtgaaagcag
    31621 cagcaggcgg ctatggtgct caccgtgaaa tgttaccaga gtttaatcta gttatccttg
    31681 cccctcaagt tgcttcaaac tttgaagata tgaaggcaga aacagataag ctcggtatta
    31741 aacttgctaa gacagaaggc gctcaataca tcaaattaac tcgtgatgga aaaggtgccc
    31801 tagcattcgt acaagagcaa ttcgattaag gctaggaact gtgaaacagt ctcccatcgt
    31861 tacggaaatc gctatggcga atttcctaat cgccttatta attcgtcggt aaaaagatat
    31921 cgtttttacc tcctcatgtc acaattcagt gacttggtac aagaagtgag atggagaagc
    31981 atggctcact gactcctctc ctctcacttt tactttattt aaatcaagaa ataggtgaaa
    32041 aaaatgacaa aaacacttcc aaaagacttt attttcggtg gcgcaacagc tgcttatcaa
    32101 gcagaaggtg ctacacatac tgatggaaaa ggaccagttg cttgggataa atatcttgag
    32161 gataactact ggtacactgc cgaaccagct agtgattttt acaatcgata tccagttgac
    32221 ctcaagctag cagaagagta tggtgtcaat ggtattcgaa tttctattgc ttggtcacgt
    32281 attttcccga ctggttacgg ccaagtaaat gctaaaggtg ttgagtttta tcataattta
    32341 tttgcagagt gtcacaaacg tcatgttgag ccttttgtaa ctcttcatca ctttgacacg
    32401 ccagaagctc tccactcaaa tggagacttc ttaaaccgtg aaaatatcga acattttgta
    32461 gactacgctg ctttctgttt tgaagaattt ccagaagtaa actattggac aacctttaat
    32521 gaaattggac caatcggtga tggtcaatat ttggttggga aattccctcc aggtatccag
    32581 tacgaccttg ccaaagtctt tcaatcacac cacaatatga tggtgtctca tgcacgcgcg
    32641 gtaaaattgt acaaagagaa aggctataaa ggggaaattg gtgttgttca cgccctgcct
    32701 actaaatatc ctctagatca tgaaaatcca gcagatgttc gtgcagctga gttggaagat
    32761 attattcaca acaaatttat tttggatgca acctatctgg gtcactactc tgaaaagact
    32821 ctggcagggg tagaggctat cttggctgcc aatggtggta gcttggatct tcgtgaagaa
    32881 gattttacag cattagaagc tgcaaaagac ttgaatgatt tcctaggaat caactactat
    32941 atgagtgact ggatggaagc ctttgatgga gaaactgaaa ttatccataa tggtaaaggt
    33001 gaaaaaggaa gctctaaata ccaaatcaaa ggtattggtc gtcgtgtagc tcctgactat
    33061 gtaccacgca cggattggga ttggattatc taccctcaag gtttgtatga ccaaatcatg
    33121 cgcgtgaaga aagattatcc taactataag aaaatctaca tcactgaaaa tggtcttgga
    33181 tataaagatg agttcgttga taacactgtt tacgatgatg gacgtattga ttacgtgaag
    33241 caacacttgg aggttttatc tgatgcgatt gcagatggag ctaatgtaaa aggttacttc
    33301 atttggtcat taatggatgt cttctcatgg tcaaacggtt atgagaaacg ttatggtctc
    33361 ttctacgtag attttgaaac tcaagaacgt tatcctaaga aatcagctca ctggtacaag
    33421 aaagtagcgg aaactcagat tatagactag tagaattagt cattagatat agaattttag
    33481 tgagtcaaaa agatgttcaa agattttatc caatctattt atgaaaaagt ttatattata
    33541 aatttcgaaa aatgctctca aataccgtgt ttgacgagtg aagaattgaa aagtcttgga
    33601 aaatggtatg tctcgactgg taaagaatgg atttgtcatt cagatgatga tctggaagaa
    33661 tttaaaaatc tatttttaaa ttttatcaat cctgaagaat agaaaaacgc atagtatcag
    33721 gtgttgaaac acctgatact atgcgtttta ttgtgggaag atttactttt tttcttctga
    33781 aattgagttg ttacccaggc tctttcagtt tattaaggct tgatgacttt aatgtgttta
    33841 gatagcttaa aaaggattga atcacttagt ttagaatctg aaacaatagt atcaagattt
    33901 gatatattat aaaaagtata aaaatcaaac ttattgaact tactatgatc tgcgagtaaa
    33961 tattttttat tagaattatt taaagcgatg cgttgagcct ctccctcttc ctcgctaaaa
    34021 gtagctagag ctccgttttg aataccatta cagctaacga aagctttaga aaattggaga
    34081 ttagagagat tttgtagggt caatgtgcca acaaaagcac ctgtaatatc gcgataattt
    34141 ccacctatca aaatcaaatc tgttaatttt cgttcgctta aaatcagaaa aacaggtaga
    34201 ctgttggtta cgacgcggat attgtcaata ggcaactcac gcgcaaaaaa ctctaatgtt
    34261 gttcctggtc caatgaaaat agtttctctt tcttctacta gactgcctgc aaaatgggct
    34321 atttcttgtt tttctgccgt ttggagggct tgtttttcaa tatttgatcg ctcattagtc
    34381 aaaagggagt tggttcgaag tttttcagct ccaccatgca cacgaatcag caaatcttta
    34441 tcagctaatt cctgtaaata gcgccttgca gtcatatctg aaacggctat ttcgtccata
    34501 atctgtttaa ctgttatggt tcctttacta tttactatct ctaaaatttt ggttaatttt
    34561 tcttgtttga gcatattatc acctcgtttc ctactactat cttaccataa acaaactcat
    34621 cattcaaata caaaaacaac aaaatgaaac aaaaacaaaa atatcgaagt ttgttttcaa
    34681 aactttcgat atttttgttg ggttataact ttgatgtttc tagtttactt tttgatgatt
    34741 gagagtgatg gagaattagt ctaaaccgta gttatagtca tcgtcttgca tggcttcaac
    34801 ttcgccaaga aggtaaccat ttccgacttg agagaagaag tcatggttgg aagttcctgt
    34861 tgaaataccg ttcataacga ttgggttgac atcttcagct gaatctggga aaagtggatc
    34921 ttgtcccatg ttcatgagag ctttattggc attgtagcga aggaaggttt taacctcttc
    34981 agtccaacca acaccgtcat aaagactctc tgtgtagcct tcttcatttt cataaagagt
    35041 atagagtagg tcgtacatcc attctttgag tttttcttgc tcttcttcag gcaattcatt
    35101 gaaaccaagt tggaatttgt aaccaatgta ggttccgtga acagactcgt cacgaataat
    35161 caatttaatg atttctgcaa cgttggctag tttgttgtta ccgagatagt agaggggagt
    35221 gaagaaacca gagtagaaga ggaaggtttc gaggaagacg ctggcaactt tcttttcaag
    35281 tgggctgccg tttaggtaga tttcgttgac aatctcagcc ttcttttgta ggtaaggatt
    35341 ggtattggtc cattcgaaaa tttcttcaat ctcagcctta gtattcaagg tagaaaagat
    35401 tgatgagtaa gatttagcgt ggacagattc cataaattgg atgttattga aaacagcttc
    35461 ctcatgtggt gtacggatgt ctgcgcgaag ggcttgaacc ccagtttcag attgcatagt
    35521 gtcaagaagg gttaaaccac caaaaacttt tccgaccaag tctttctctt tgttagatag
    35581 ctttctccag tcatccaagt cgtttgataa gggaatacgt gtatcgagcc aaaattgctc
    35641 cgtcagtttt tcccaagttg atttgtcgat gacatcttcg atggcattcc agttaatggc
    35701 tttgtagtaa gtttccattt aaaatctctt tctgtgttta gtattgcgaa ctcacaatta
    35761 tttctacttt accataattc tataggagta tcgcacaaaa agtcggaagc ccgactttta
    35821 aaatgttaca taaattatgt tatgacatag tagatttgat tttatcagtg ctgcttaggg
    35881 aaaaatagtg tttctatgat agaaactaaa tcacacagct ttcacattgg ttggcgccga
    35941 cttctccacc gtcatctgta aaggtacgga cgtagtagat agacttgatt cccttgttaa
    36001 aggcatagtt acgaaggatg gacaagtcac gtgtcgtttg tttattttcc ctcttccatt
    36061 cgtaaaggcc ttttggaatg tcactacgca tgaagagggt gagtgaaagt ccttgatcca
    36121 cgtgttcagt cgcagcagcg taaacatcga tgactttacg catatccata tcgtaggcag
    36181 aagtgtagta aggaatggtt tctgtagaca agccagcagc agggtagtag attttaccaa
    36241 ttttcttctc ttggcgttct tcgatacgtt gcgtaattgg gtggatagaa gcagaaacgt
    36301 cgttgatata gctgatagaa ccatttggcg ctacagcaag gcgattttgg tggtaaagac
    36361 catcttcttg aaccttgtcg cgaagttcag cccaatcagc aacaccaggg ataaagacat
    36421 ttttgaagag ttctttaaca cggtctgatg ttggaacaaa ttcaccagtt acatacttgt
    36481 caaagtaact tccgttagca tagtctgatt tttcaaagtt gtggaaggta ataccacgtt
    36541 cacgcgcaat attgtttgac tctaccaagg tccagtagtt cataagcata aagtagatgc
    36601 ttgtaaattc aacagactca ggtgaaccat attcaatgag ttgttgggca aggtagctgt
    36661 gcagtcccat ggcaccgaga ccaaaggtgt gggcttggct atttccatgg tcaatcgttg
    36721 gtacagctac gatatgtgaa ctatctgtaa cgaaagtaag ggcacgaacc atagcacgga
    36781 tagaacgacc aaaatcaggt gaagtcatca tgttaaccac gttggttgaa cccaggttac
    36841 atgaaacatc tgttcccatt tgaaggaatt cttgagcatc gttgatcaag cttggttctt
    36901 gaacttgaag aatctcagaa cacaagttac tcatgataat ctttccatca acaggatttg
    36961 cacggttagc cgtatcgatg ttgactacat aaggatagcc agactcttgt tgcaatttag
    37021 agatttcagt ttccaaatcc cgcgccttga tttttgtctt gcgaatattt ggatttgcga
    37081 ccaattcatc gtatttttca gtaatgtcga tgtaattgaa tggcacaccg tattcttttt
    37141 ctacagagta agggctgaag aggtacattt cttcattttt acgagccaat tcgtagaatt
    37201 tatcaggtac tacaacacca agtgatagag tcttgacacg tactttttca tcagcgtttt
    37261 ctttcttagt tgaaaggaaa gcgatgatat ctgggtgaaa gacgttgagg tagacaacac
    37321 cggcaccttg acgttgcccc aattggttgg agtaagagaa gctgtcttca aaaagcttca
    37381 tgacaggaac gacacctgaa gcagctcctt catagccttt gataggtgca ccagcttcac
    37441 gaaggttgct gagggtaatt cccacaccac caccaatacg tgaaagttga agagctgagt
    37501 tgatagaacg tccgatagag ttcatatcat ccgtcacttg gattaggaaa caagatacca
    37561 actccccacg acgagcacgt ccagcattca agaaggaagg agtagcaggt tggtagcgtt
    37621 ggtggatgat ttcattggca atatcgattg caacagcttc attcccatca gcgaaataaa
    37681 gggcgttgaa gaagacacgg tcttccatat tttcaagata gtattcaccg tcattagtct
    37741 ttaaggcata ttgattgtaa aatttataag ctgccatgaa tgacttgaat tggaagtttt
    37801 ggtctttgat aaattgagcc aattcttcca agaactctgg acggtatttc tttataaagg
    37861 ctgtttcgat gtagttgtgt tcaatgaggt aattgatttt gtctttgatt gaatcaaaaa
    37921 ccatagtgtt tggaactaca ttttctttaa agaaagcatc caaggcttcc ttatctttat
    37981 gaagcatgat ttgtccatta acaggacggt taatttcgtt attaagacgg aagtaagtca
    38041 cgtcttcaag atgttttaat cccataaaat ttcccttatc taattacaaa agaaaggctt
    38101 ctaagttagc cctaaaagca gtttcttctg gatgatgtac taagattatg ctaattgttt
    38161 cagttttcct ggttggaaac ctgaaaagac ttcagttggt gtttggataa caggagctgc
    38221 gctaaaaccg agctctttaa cttgatcgac gtactcaggt tgctcatcaa gattgatttc
    38281 acgataagag acattattac tgtccaagaa acgcttggtc attttacatt ggacacaatt
    38341 gtttttagaa taaacggtta ccattgtgta actcctcttc aaaatttaat actatcttag
    38401 tatatcagaa aataaaattt tgtcaatgat ttaaactaaa tatagtggtg ttcttttgtt
    38461 gttttcatat ataaacacaa tatatagtgt ttgtattgtg aaatagcaat gattatccta
    38521 taaattgttt ttgaagaaac tatatcctgt gttttgttat ctaggataga agattttcag
    38581 caagttatct gaaaggaaat tgaaggaaaa tccagaaaat gcttgaaaaa aatcctagaa
    38641 gatggtataa tactaaattg taagggttat cacatataac tcaaaaaaag aaagaacaaa
    38701 aggagagtca aactatggct tctaaagatt tccacgtagt ggcagaaaca ggtattcacg
    38761 cacgtccagc aacattgttg gtacaaactg ctagcaaatt tgcttcagat atcactcttg
    38821 agtacaaagg taaatcagtt aaccttaaat caattatggg tgttatgagt cttggtgttg
    38881 gtcaaggtgc tgacgtaact atctcagctg aaggtgcaga tgcagatgac gctatcgctg
    38941 caatctcaga aacaatggaa aaagaaggat tggcataagg gaaatgacag aaatgcttaa
    39001 aggaatcgca gcatctgacg gtgttgcagt tgcaaaagca tatctactcg ttcagccgga
    39061 tttgtcattt gagactatta cagtcgaaga tacaaacgca gaagaagctc gccttgatgc
    39121 cgctctacag gcatcacaag acgagctttc tgttattcgc gagaaagcag taggtacgct
    39181 cggtgaagaa gcagctcaag tttttgatgc tcacttaatg gttcttgctg acccagaaat
    39241 gatcagccaa atcaaggaaa ctatccgtgc gaagaaagtg aatgcagaag caggtctgaa
    39301 agaagttaca gatatgttta tcactatctt tgaaggcatg gaagacaacc catacatgca
    39361 agaacgcgca gcggatatcc gcgacgtgac aaaacgtgta ttggcaaacc ttcttggtaa
    39421 aaaattgcca aacccagctt ctatcaatga agaagtgatt gtgattgcgc atgacttgac
    39481 tccttcagat acagctcaat tggacaaaaa ctttgtaaaa gcttttgtaa ccaacatcgg
    39541 tggacgtaca agccactcag ctatcatggc acgtacactt gaaattgctg ctgtattagg
    39601 tacaaataac atcactgaaa tcgttaaaga cggtgacatc cttgctgtta acgggatcac
    39661 tggtgaggtt attatcaacc caactgatga acaagcggca gaatttaaag aagctggtga
    39721 agcctatgca aaacaaaaag ctgaatgggc gctcttgaaa gacgctaaaa cagtgactgc
    39781 tgacggtaaa cacttcgagt tggctgctaa tatcggtact ccaaaagacg ttgaaggtgt
    39841 taacaacaac ggtgcagaag ctgttggact ttaccgtaca gagttcttgt acatggattc
    39901 tcaagacttc ccaactgaag atgagcagta tgaagcatac aaggctgttc ttgaaggaat
    39961 gaacggtaaa cctgttgtcg ttcgtacaat ggatatcggt ggagataagg aacttcctta
    40021 cttcgatatg cctcacgaaa tgaacccatt ccttggattc cgtgctcttc gtatctctat
    40081 ctctgagact ggagatgcta tgttccgcac acaaatccgt gctcttcttc gtgcgtctgt
    40141 tcacggtcaa ttgcgtatca tgttcccaat ggttgcgctc ttgaaagaat tccgtgcagc
    40201 gaaagcagtc tttgatgaag aaaaagcaaa ccttcttgct gaaggtgttg cagttgcgta
    40261 taacattcaa gttggtatca tgatcgagat tcctgcagcg gctatgcttg cagaccaatt
    40321 tgctaaagaa gttgacttct tctcaattgg tacaaatgac ttgatccaat atacaatggc
    40381 agcagaccgt atgaacgaac aagtttcata cctttaccaa ccatacaacc catcaatcct
    40441 acgcttgatt aacaatgtga tcaaagcagc tcacgctgaa ggtaaatggg ctggtatgtg
    40501 tggtgagatg gctggtgacc aacaagctgt tccacttctt gtcggaatgg gcttggatga
    40561 gttctctatg tcagcaacat ctgtacttcg tacacgcagc ttgatgaaga aactcgacac
    40621 agctaagatg gaagagtacg caaaccgtgc ccttacagaa tgctcaacaa tggaagaagt
    40681 tcttgaactt caaaaagaat acgttaattt tgattaatcg aaaagtccct gcaactcagt
    40741 tacagggatt tttttgatat tttaaaaaga attttcaaga aaatctttct tatagaaagt
    40801 ccaaccttga aaaagtagtg gtcagaacaa aaaatactta aatggttcat aaaattcttg
    40861 acaagttgga gatttaggag taaactatta accagttaag taatagaggg gagtttctgc
    40921 aatttagaaa tgaattgcaa ctagaaatat caaatagaaa gagagtttcg atgaaaatta
    40981 ataagaaata ccttgttggt tctgcggcag ctttgatttt aagtgtttgt tcttacgagt
    41041 tgggactgta tcaagctaga acggttaagg aaaataatcg tgtttcctat atagatggta
    41101 aacaagcagc acaaaaaacg gagaatctga ctcctgatga ggtcagtaaa aaagaaggta
    41161 tcaatgccga acaaatcgtc atcaagataa cagaccaagg ctatgtcact tcacatgggg
    41221 accactatca ttattacaat gggaaggttc cttatgacgc tatcatcagt gaagaattgc
    41281 tgatgaagga tccaaactac cagctaaagg acgaggatat tatcagtgaa atcaagggtg
    41341 gttatgttat caaggtagat ggaaaatact atgtttacct taaggatgca actcatgcgg
    41401 ataatgtccg tacaaaagaa gaaatcaatc gacaaaaaca agaacatagt cagcatcgtg
    41461 aaggagggac ttcagcaaac gatggtgcgg tagcctttgc acgttcacag ggacgctaca
    41521 ccacagatga tggttatatc tttaatgctt ctgatatcat tgaggacacg ggtgatgctt
    41581 atatcgttcc tcacggcgac cattaccatt acattcctaa gaatgagtta tcagctagcg
    41641 agttagctgc tgcagaagcc ttcctatctg gtcggggaaa tctgtcaaat tcaagaacct
    41701 atcgccgaca aaatagcgat aacacttcaa gaacaaactg ggtaccttct gtaagcaatc
    41761 caggaactac aaatactaac acaagcaaca acagcaacac taacagtcaa gcaagtcaaa
    41821 gtaatgacat tgatagtctc ttgaaacagc tctacaaact gcctttgagt caacgccatg
    41881 tagaatctga tggccttgtt ttcgacccag cgcaaatcac aagtcgaaca gccagaggtg
    41941 tagctgtccc tcatggtaac cattaccact ttatccctta tgaacaaatg tctgaattgg
    42001 aa
//
LOCUS       DBERDK010000011        40109 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.011, whole genome shotgun sequence.
ACCESSION   DBERDK010000011 DBERDK010000000
VERSION     DBERDK010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 40109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40109
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.011"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(102..1586)
                     /locus_tag="ACL73L_000549"
     CDS             complement(102..1586)
                     /locus_tag="ACL73L_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003096885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator PRD
                     domain-containing protein"
                     /protein_id="HGS0771579.1"
                     /translation="MRNLLSTKVQRQLRLMETLIQNRNWMKLHELAEKLGCTERILKS
                     DLNELRIAFPSINIQSSVNGIMIDLEVNTSVEDIYQYFLANSQSFQLLEYMFFNEGLP
                     IYRTIENLYFSSANLYRLGRNITKVLSSQFQIELSFTPSEIRGNEIDIRYFFAQYFSE
                     RYYFLDWPFPDLPEEDLTEFADFFYKITNYPMRFSIYRMYKLMIAISIHRVKNGHFID
                     LPNHFYKEYYPLLKSIPNFQETLAYFSKHFGLEMTPDTIAQIFISFLQNDIFLDPQEF
                     FNSLEDNSQARYSYQLLSQILEGLSKQYKITFTNHDELIWHLHNTAFFERQEIFSTPI
                     LFEQKALTIKKFEVYFPDFMGSARQELAQYRQAIGQHDHPEQLEHLMYTILTHAENLS
                     TQLLENRPPIKVLIISNFDHAISLTFVDMLSYYCNNRFTFDIWDELKTSPEILNQTDY
                     DIIVSNFYIPGITKKFICRNHLSIMNLVNHLNTLSNEIHLSNTL"
     gene            1802..8920
                     /locus_tag="ACL73L_000550"
     CDS             1802..8920
                     /locus_tag="ACL73L_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HGS0771580.1"
                     /translation="MKHSHKKSFDWYSMQQRYSIRKYYFGAASVLLGTALVLGAAASV
                     QTVQAEENKQETTNSISVGRGEAATKPAEVSASNKEKTYAAPTVANPVETTPVKTEEV
                     TKPAEKVEEAKDKKEEVTHQDAVDKSKLLTALSRAKKLESKLYTEASAANLQTSIQAG
                     QSLLGKADATEAELSAAESSIQSFIIGLELRSNSNKETVSETPVAKKADAVESKEGAK
                     PAATTERSAVDSAILPTSTADKVETTSAPASINEILKLGLSLSDARQNPAIRKEDVNR
                     GYSGFRAASNPANPIVSGSGNTVAFADISQGGRSYSFRGYGNSRGGNSIHYDVTTVRS
                     GNSVNFTISYSAPGDSREFVNNNFILDKGDGFGNPSNATITSSNPIVREQSKSISQGA
                     NYVSHSGYSMTSAISTNTEQTIRFSLPIINLNGDLSVRLKPVTFNVDQGGGGAATSND
                     PYSNSNYYYRANPLYLDANPYGGTNNKTVSEDIDFQTVDLPTSKLPEGQTRLVREGEK
                     GQRQITYKVHRFGNETLLGLPISNSVTKEAKPRIMQIGVAKDLIDTVKPRVDQNKVGD
                     TNNLTFYLDNDGNGVYTEGVDELVQKIAIKDGAKGEKGAQGERGLTGAKGEKGDRGER
                     GLTGAQGAKGEKGAQGERGLTGAQGAKGEKGDQGERGLTGAQGAKGEKGAQGERGLTG
                     TQGAKGEKGDRGERGLTGTQGAKGEKGDRGERGLTGAQGAKGEKGARGERGLTGAQGA
                     KGEKGAQGERGLTGAQGAKGEKGDQGERGLTGAQGEKGDRGERGLTGAKGEKGDQGER
                     GITGAKGEKGAQGERGLTGAKGEKGDQGERGLTGAQGEKGDRGERGLTGAKGEKGAQG
                     QAGRDGVTPTVTVKDNKNDGTHTITINDGRGNVTSTVVRDGFDGASPLVATQRNDADK
                     TTTVIFYYDKNGNNELDASDKKLKEVVIADGAKGEKGDKGEQGLQGRDGEQGPKGEDG
                     KTPTVKVTDGQDGTHTITINDGKGGITTTVVRDGFDGASPLVSTHRNEADKTTTVIFY
                     YDLNDNNQFDEGDTKLKEVVIADGKQGPKGDKGDNGKDGFTPEVTVTDNNNGTHTITI
                     TQPDNRPSLTTIVKNGEDGKTPKVKAERDDAKKQTTLTFYIDKDGDGSYTAGKDELVQ
                     TTVVKDGQDGAAGASGRDGKEVLNGKVDPTTEGKDGDTFVNTQTGDVFVKKGNTWEPA
                     GNIKGPKGDKGADGAKGEKGAQGERGLTGAQGVKGEKGDQGERGLTGSKGEKGAQGER
                     GLTGAQGAKGDKGEQGLQGRDGAQGPKGADGQRGPAGPQGPKGEQGNPGTPGKDGKSL
                     IAVKNGTETKVYVEDPARPGQPLNPNQPLATITDGRNGTNGQSPTITATRSVQNGKNG
                     VLVTITPVGGRPQTTFVEDGQKGADGKTPTVTITEGQNGTHTLTVHNPGSPDVTTTIR
                     DGATGQAGRDGKDVLNGKVNPQPNQGKNGDKYINTETGDVYVKNNGNWDKEGNIKGPK
                     GDKGADGAKGEKGDQGERGLTGAQGAKGADGAVGRDGRDGKDVLNGKANPEAHQGKDG
                     DKYVNTETGDVFVKNNGNWDKEGNIKGPKGDKGERGEDGKTPEVTVTPGKDGHSTDIT
                     FTVPGKDPVTVNVKDGENGLNGKTPKVDLLRVQGKNGNPSHTIVTFYTDENNDGKYTP
                     GTDELLGSEMIKDGAKGADGRDGKSLLTVKDGKETKVYQEDPANPGQPLNPEKPLAVI
                     RDGVDGKSPTVTAVRKDEAGHKGVEITVDNHDGSQPTTVFVQDGAKGETGATGQDGQT
                     PTITTQRGQDGQSTVVTITTSGKDPVTFTVKDGKNGKDGRAPKIKVEDITSPSRIRRD
                     TDAAATPTRNGIRVTVYDDVNDNGVYDEGVDKVLNSKDIYNGIDGRDGSAPTITTKDN
                     GDGTHTITVQNPDGSESTTVVKDGKDGKTANITTTENPDGSHTITVTNPDGSTKETVV
                     KNGKDGKTPKVEVTDNNDGTHTVKVTDGDGNVTNAIIKDGKDGKAATATTTENPDGSH
                     TVTITNPDGTKNEFVVKNGRDGVDGRTPTASVRDNGDGSHTIVITNPEGVTTETTVRD
                     GKSPKVTITDEQNGTHKISVLNGDGTTTTTTVRDGREPKLEVIDNNDGSHTIKVTGAD
                     GKGTTTTIFDGKSPKANIVDNGDGTHTLTIVDSDGREYKSIIKDGKDGKDSVSPTVTV
                     KNNNDGTHVVTITNPDGSKTEMVIKDGKDGKSPKVSVEDNGDGSHTITIINSDGTVTK
                     TVIKDGRDGRDGRDGKDGKDGKDGKCGCQDKPVTPSNDKPVPPTPNVPTPEVPVKPVP
                     AVPEQPVVPTPAQPATPVNANPVAPTTGKENRGDKLPETGSQSDYISVLLGSGILLSL
                     YVGRRKEA"
     gene            complement(9157..9705)
                     /locus_tag="ACL73L_000551"
     CDS             complement(9157..9705)
                     /locus_tag="ACL73L_000551"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HGS0771581.1"
                     /translation="MSKKVLFIVGSLRQGSFNHQMALEAEKALAGKAEVSYLDYSALP
                     LFSQDLEVPTHPAVAAAREAVLVADAIWIFSPVYNFSIPGTVKNLLDWLSRALDLSDT
                     RGVSALQDKFVTVSSVANAGHDQLFAIYKDLLPFIRTQGVGDFTAARVNDSAWADGKL
                     VLEETVLNSLEKQAQDLVEAIQ"
     gene            10026..10514
                     /locus_tag="ACL73L_000552"
     CDS             10026..10514
                     /locus_tag="ACL73L_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HGS0771582.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISGSFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEQADQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGVSQEKAETVSKEKGAGEIDKITFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GL"
     gene            10511..11698
                     /locus_tag="ACL73L_000553"
     CDS             10511..11698
                     /locus_tag="ACL73L_000553"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HGS0771583.1"
                     /translation="MKLSNRVLEMEESVTLAAGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQDAAIASIRDGRASFYTVTSGLPELKAAVNSYFERFYGYSVASNQVTVAAGAKY
                     SLYTFFMAVVNPGDEVIIPTPYWVSYGDQVKMAEGVPVFVSAKEDNHFKVTVEQLEAA
                     RTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEERLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVTDFTTVILEEAEVALVTGAGFGAPENVRLSYATDLDTLKEAVERLKAFMGS
                     END"
     gene            11691..12056
                     /locus_tag="ACL73L_000554"
     CDS             11691..12056
                     /locus_tag="ACL73L_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGS0771584.1"
                     /translation="MIDHFEIKVKDLQISEGFYRSFLAPLDYKLAFKTSSLISFLSPN
                     SPHPGGDFWLTQGTQDPVHFAFLAENKEEVQACYEAGLEAGGRDNGAPGYRSEHPIYY
                     AAFMIDLDGNNIEVVCHKE"
     gene            12072..13418
                     /gene="asnS"
                     /locus_tag="ACL73L_000555"
     CDS             12072..13418
                     /gene="asnS"
                     /locus_tag="ACL73L_000555"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HGS0771585.1"
                     /translation="MMTKRVTIIDVKDYVGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIIYATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT
                     FVMNYPAAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     GMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            13571..13861
                     /gene="rpsF"
                     /locus_tag="ACL73L_000556"
     CDS             13571..13861
                     /gene="rpsF"
                     /locus_tag="ACL73L_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HGS0771586.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIQDFREGLYHIVNVEANDDAALKEFDRLSKINADILRHMIVKIDA"
     gene            13873..14343
                     /gene="ssbA"
                     /locus_tag="ACL73L_000557"
     CDS             13873..14343
                     /gene="ssbA"
                     /locus_tag="ACL73L_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HGS0771587.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRTFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LESRSVREGHTGGAYSAPTANYSAPTNSVPDFSRNENPFGATNPLDISDDDLPF"
     gene            14375..14614
                     /gene="rpsR"
                     /locus_tag="ACL73L_000558"
     CDS             14375..14614
                     /gene="rpsR"
                     /locus_tag="ACL73L_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HGS0771588.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
     gene            14909..16309
                     /locus_tag="ACL73L_000559"
     CDS             14909..16309
                     /locus_tag="ACL73L_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HGS0771589.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRNSKTGFIPTTIPTVFKQLRE
                     KGILTGIASGRGIFGVVPEIRDLKPDFFVTLNGAYIEDKKGQVIYQHQIEKSDVEEYI
                     SWAKQEGIEYGLVGSHDAKLSTRTDMMSEAINPIYPDLDVDPDFHEKEDIYQMWTFED
                     KGDDLHLPDSLSDKLRMVRWHQHSSDIVPISGSKATGVEKVVEHLGLKPEKVMVFGDG
                     LNDLELFDYAGISVAMGISHDKIKEKADYITKTLEEDGIFAALEVFGMVEKELHFPQV
                     DIETVEGPLATIKTNHGDLRIKLFPEHAPKTVANFVYLSKDGYYDGVIFHRIIKDFMI
                     QGGDPTGTGMGGESIYGESFEDEFSEELYNIRGALSMANAGPNTNGSQFFIVQNQHLP
                     YSKKEITRGGWPEPIAEIYANQGGTPHLDRRHTVFGQLADEASYAVLDAIAAVETGAM
                     DKPVEDVVIETIEIED"
     gene            16311..16670
                     /locus_tag="ACL73L_000560"
     CDS             16311..16670
                     /locus_tag="ACL73L_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HGS0771590.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEEKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHHLKKKK"
     gene            16724..17473
                     /locus_tag="ACL73L_000561"
     CDS             16724..17473
                     /locus_tag="ACL73L_000561"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HGS0771591.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDFCAGNGAVGLFASTRTQAQILSVEIQERLADMAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVDPHSNLNESEHYLLARHEITTNLEEICHSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKREKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            17463..17762
                     /locus_tag="ACL73L_000562"
     CDS             17463..17762
                     /locus_tag="ACL73L_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HGS0771592.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDVRRRLAIHNSGKGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALLKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            17833..18768
                     /locus_tag="ACL73L_000563"
     CDS             17833..18768
                     /locus_tag="ACL73L_000563"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HGS0771593.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRVITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTESFNA"
     gene            18855..19367
                     /locus_tag="ACL73L_000564"
     CDS             18855..19367
                     /locus_tag="ACL73L_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HGS0771594.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGVVCQEPADFFESNHLYDLYVYPEMDIQSVKERACRHLGAPYNASFYPDAAGFYCSQ
                     YIAEILPIFETIPMKFGDGEQEISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERNLHDSDF"
     gene            19351..19999
                     /locus_tag="ACL73L_000565"
                     /pseudo
     CDS             19351..19999
                     /locus_tag="ACL73L_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614493.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
     gene            20100..20294
                     /locus_tag="ACL73L_000566"
     CDS             20100..20294
                     /locus_tag="ACL73L_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HGS0771595.1"
                     /translation="MSLENKLEQATGAVKEGFGKVTGDSKTELEGAVEKTVAKAKDVV
                     EDAKGAVEGAVEGLKNVFTK"
     gene            complement(20340..21785)
                     /locus_tag="ACL73L_000567"
     CDS             complement(20340..21785)
                     /locus_tag="ACL73L_000567"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HGS0771596.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVIFDSISYDSRK
                     VTEDTLFFAKGAAFKKEYLLSAITQGLAWYVAEKDYEVGIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKLLAFTGTKGKTTATYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALT
                     TPESIDLFDMMNQAVQNDRTHLIMEVSSQAYLVHRVYGLTFDVGVFLNITPDHIGPIE
                     HPSFEDYFYHKRLLMENSRAVIINSDMDHFSVLKEQVEDQDHDFYGSQFDNQIENSKA
                     FSFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGTTGNKGESRRKDFGLLLN
                     QHPEIQVFLTADDPNYEDPMAIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGADCYQIIQGKKESYPGDTAVAENYL"
     gene            21891..23513
                     /locus_tag="ACL73L_000568"
     CDS             21891..23513
                     /locus_tag="ACL73L_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HGS0771597.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGAY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVAKYNTMREEEHSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLIPIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRVFETGDKINSKRLLVDTIKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYVKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPLYTVFYGKPDSLAMGLFVFAVLQSIILGLYMVLSPM
                     LQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLWSFFYVALVGAMGG
                     GLYMVMSLRTYLLDKVIGKVQADRLRAKFKLS"
     gene            23718..25676
                     /locus_tag="ACL73L_000569"
     CDS             23718..25676
                     /locus_tag="ACL73L_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HGS0771598.1"
                     /translation="MKKSKSKYLTLAGLVLGTGVLLSACGNSSTASKTYNYVYSSDPS
                     SLNYLAENRATTNDIVTNLVDGLMENDQYGNYVPSLAEDWTVSQDGLTYTYKLRKDAK
                     WFTSEGEEYAPVTAQDFVTGLQYAADKKSEALYLVQDSVAGLDDYITGKTSDFSTVGI
                     KALDDQTVQYTLARPEPYWNSKTTSTILFPVNADFLKSKGDDFGKVDPANILYSGPFL
                     MKAFVSKSVIEYKKNPNYWDAKNVFVDDVKLTYYDGSDQESLERNFTAGAYTTARLFP
                     NSSSYEGIKEKYKNNIIYSMQNSTSYFFNFNLDRKSYNYTSKTSDIEKKSTQEAVLNK
                     NFRQAINFAFDRTSYGAQSEGKEGATKILRNLVVPPNFVSIKGKDFGEVVASKMVNYG
                     KEWQGINFADGQDPYYNPEKAKAKFAEAKKELEAKGVQFPIHLDKTVEVTDKVGIQGV
                     SSIKQSIESVLGSDNVVIDIQQLTSDEFDSSGYFAQTAAQKDYDLYHGGWGPDYQDPS
                     TYLDIFNTNSGGVLQNLGLEPGEANDKAKAVGLDVYTKMLEEANKEQDPAKRYEKYAD
                     IQAWLIDSSLVLPSVSRGGTPSLRRTVPFAAAYGLTGTKGVESYKYLKVQDKIVTTDE
                     YAKAREKWLKEKEESNKKAQEELAKHVK"
     gene            25887..26621
                     /locus_tag="ACL73L_000570"
                     /pseudo
     CDS             25887..26621
                     /locus_tag="ACL73L_000570"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            26808..27530
                     /locus_tag="ACL73L_000571"
     CDS             26808..27530
                     /locus_tag="ACL73L_000571"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0771599.1"
                     /translation="MNFNHVYFGYDENRPVLKDITCSIFKGQKIAFVGPSGSGKSTIV
                     RLLEQFYKPLSGDILMEQSSIYDFNLKEWRSKIAWVSQNNAVLSGSIRDNLCLGLNRL
                     VTDDELMKVLDLVSLGDEIRSMKEGLDTEVGERGRLLSGGRSQRLQIARAYLKDAEIL
                     IFDEATANLDADSEYAIISSLYSVLKEKTVVIIAHRLSTVKDVDCIFFLEEGKITGSG
                     THKELLENHERYARFVQEQMIE"
     gene            27688..28782
                     /locus_tag="ACL73L_000572"
     CDS             27688..28782
                     /locus_tag="ACL73L_000572"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HGS0771600.1"
                     /translation="MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVLAAIESADYALATSSGMSAIVLAFSVFPVGSKVLAVRDLY
                     GGSFRWFNQVEQEGHFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
                     LAHAKGAKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELY
                     EKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLY
                     TGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHS
                     YGLTDDLLRLSIGIEDARDLIADLRQALEG"
     gene            28790..29956
                     /locus_tag="ACL73L_000573"
     CDS             28790..29956
                     /locus_tag="ACL73L_000573"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HGS0771601.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVVLPE
                     IHQAVQTYADQLVYGYTYASEDLIKEVQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFARSVKLNNRRLITNSLVEKDSLFEIDFDQLEKDLVEEE
                     VKLYVLCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNIAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVVDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNEAKVILNRGLDFGEEGSLHARINIAMPKSLLQEVCQRIVATFAKC"
     gene            30049..33147
                     /locus_tag="ACL73L_000574"
     CDS             30049..33147
                     /locus_tag="ACL73L_000574"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGS0771602.1"
                     /translation="MAKLIPGKIRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLIFCACDFFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLDRILSPKSECAYELSAVGQVEAGTNHILWTLRIGQINSQKYYVIRDIPLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQELLTFLHGLTEEGLVPDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPHFQFDHQVDSYQTLLFQDMHADANLFAFTVTEYSDYFEMEISES
                     PRVNVFYQGAVLFHKGQVYFLTDQQMRLLKEIKALPVGQQGKKYLQFDSSDRDKLASC
                     LTLFIQMGPVSAPERLQIKTFAPSFYFDREEDNRIRLEIQFDYGNRQVSSRQELEELP
                     FSSDADLEERIFQVCLAAGFEADFQSWRQALKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQDIAQEEIDQAMQALVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQELGQFELKDGTLQARKSLAYSLAHLFEGRDRVSFSQEFQNLAQDL
                     THPEDFPLQATQVKADLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFLTS
                     QVTKESRVLILAPSGLIYNWADEFQKFAPQLDVTVVHGLKASREEILAESHQIYVTSY
                     ATFRQDSEFYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENHLG
                     ELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVVYKN
                     ELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPALFMEDYQ
                     GASGKLDSLRDLLVQVADGGHRVLIFSQFKGMLEKIEQELPDLGLTSFKITGSTPAKE
                     RQDMTKAFNQGERDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAIGRAHRMG
                     QEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGISEA
                     ST"
     gene            33195..33812
                     /locus_tag="ACL73L_000575"
     CDS             33195..33812
                     /locus_tag="ACL73L_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HGS0771603.1"
                     /translation="MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPIAEEKPVKPIEKQVEKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     DFSLARRHSQPSLVRQGNQPTAPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSAPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE"
     gene            33823..35157
                     /gene="murC"
                     /locus_tag="ACL73L_000576"
     CDS             33823..35157
                     /gene="murC"
                     /locus_tag="ACL73L_000576"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HGS0771604.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADQNGISYKRYHEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQITKGLFVYGEDAELRKITSDA
                     PIYYYGFEAEGNDFVASDLLRSITGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFAGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQKY
                     PSKEIVAVFQPHTFTRTIALLDDFAHALNQADAVYLAQIYGSAREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNLTSNVQ"
     gene            35167..35631
                     /locus_tag="ACL73L_000577"
     CDS             35167..35631
                     /locus_tag="ACL73L_000577"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS0771605.1"
                     /translation="MEIPIKIIQASKSDLPEIGALQTSSFPAEKQQLSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQCLKVHSLVIESDHQRQGLGTLLLAALKEVAV
                     ELDYKGIRLESPDELLSYFEMNGFVDEEATLLYATSQGYSMIWFNPFYLEEQ"
     gene            35628..36122
                     /locus_tag="ACL73L_000578"
     CDS             35628..36122
                     /locus_tag="ACL73L_000578"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS0771606.1"
                     /translation="MKIRQARLEDLDRIVELEFENFSVEEAIPPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVGLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            36203..37858
                     /gene="mltG"
                     /locus_tag="ACL73L_000579"
     CDS             36203..37858
                     /gene="mltG"
                     /locus_tag="ACL73L_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HGS0771607.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSTEELMADSLSTVEEIMRKAPTVPTHPSQGVPASPADEIQRETPGVPSHPSQDVPSS
                     PAEESGSRPGPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGPETPTPATETVDI
                     IRDTSRRSRREGAKPVKPKKEKKSHVKAFVISFLVFLALLSAGGYFGYQYVLDSLLPI
                     DANSKKYVTVGIPEGSNVQEIGTTLEKAGLVKHGLIFSFYAKYKNYTDLKAGYYNLQK
                     SMSTEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDQIAQTVGQLQGDFKESLTAEAF
                     LAKVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDE
                     MLAAMDKNLSPYYSTIKSKNLTVNELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRDM
                     PLQSNIAILYAQGKLGQNISLAEDVAIDTNIDSPYNVYKNVGLMPGPVDSPSLDAIES
                     SINQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            37929..38411
                     /gene="greA"
                     /locus_tag="ACL73L_000580"
     CDS             37929..38411
                     /gene="greA"
                     /locus_tag="ACL73L_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HGS0771608.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVTIQEIGEDE
                     EEVYIIVGSAGADAFAGKVSNESPIGQALIGKKTGDTATIETPVGSYDVKILKVEKTA
                     "
     gene            38920..39318
                     /locus_tag="ACL73L_000581"
     CDS             38920..39318
                     /locus_tag="ACL73L_000581"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS0771609.1"
                     /translation="MENKESHKSQEIGNLIRVYNRSKREEAESEPLNLYVEDEKGNLL
                     AGLIAETFGNWLEIEYLFVKEELRGQGIGSKLLQQAESEAKNRNCCFAFVNTYQFQAP
                     DFYQKHGYKEVFSLQDYLYIRQRYYYQKNL"
     gene            39739..>40109
                     /locus_tag="ACL73L_000582"
     CDS             39739..>40109
                     /locus_tag="ACL73L_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGS0771610.1"
                     /translation="MQDNYTTKAKHLTIDSRRLIERWKKEGKSNREIASLLGKAPQTI
                     HTEIKRGTVRQCLGKGRFKEVYSADYAQQSYENNRKRSVKKSSLTKELKEKILHYHNQ
                     KFSPEMMVMAKGVNVGISTIY"
BASE COUNT        12698 a         7337 c         8987 g        11087 t
ORIGIN      
        1 ctacaataaa ataggctcca taatatctat agtggattta cccactacaa atattataga
       61 gccagaaaag tcagcacgat tatgctgact tttttgataa cttataaagt attggacaaa
      121 tgaatctcat ttgataaggt gttaagatga ttaaccaaat tcatgattga cagatgattt
      181 cggcaaataa acttcttggt aatccctgga atatagaaat tagacacgat gatatcgtaa
      241 tctgtctgat ttaaaatctc aggacttgtt tttaattcat cccaaatatc gaaggtaaag
      301 cggttattac agtaatagga aagcatatca acaaaggtaa gggatatagc atggtcaaag
      361 ttactgataa tcaaaacctt gataggtggt cgattttcta acaactgagt agagaggttt
      421 tcagcatggg ttaagatggt gtacatcaag tgttccaact gttcaggatg gtcatgctgt
      481 ccaattgcct gacggtattg agccaattct tgacgcgcac ttcccataaa gtctgggaag
      541 taaacttcaa attttttaat cgtcaatgct ttttgttcaa ataagattgg ggtagaaaag
      601 atttcctgtc tttcaaagaa agcagtgttg tgtaaatgcc aaatcagctc atcgtggtta
      661 gtaaaagtaa tcttatattg ttttgatagg ccttctaata tttgactaag taattggtaa
      721 gagtatcttg cttgactgtt atcttctaaa gagttgaaga attcttgagg atctaggaaa
      781 atatcattct gaagaaaaga aataaaaatt tgagcgattg tatctggagt catttcaagc
      841 ccaaaatgtt tagagaaata agcaagtgtc tcctggaaat tcggaatgct ctttaaaaga
      901 ggataatatt ctttgtaaaa gtggttgggg aggtcaatga agtgaccatt tttaaccctg
      961 tggatactga tagctatcat cagtttgtac attctataaa ttgaaaatcg cattggataa
     1021 tttgtgattt tatagaagaa gtcagcaaac tctgttaaat cttcctcagg aaggtcagga
     1081 aaaggccaat ctaggaaata atagcgctca gaaaagtatt gggcaaaaaa gtaacgaata
     1141 tcgatctcat ttccacgtat ctcggatgga gtaaaagata attcaatttg aaactgagat
     1201 gaaagaacct ttgtaatatt tctgcccagt cgatagaggt tggcactact aaagtaaaga
     1261 ttttctattg ttcgataaat aggtagcccc tcattgaaga acatatactc tagtaattga
     1321 aaagattgag aattagcaag aaagtattga taaatatctt ctacactagt gttaacttct
     1381 aaatctatca tgattccgtt aacagaagat tggatgttaa tagatggaaa ggcaatacgt
     1441 aattcattta aatcactttt taagattcgt tctgtacatc ctaatttttc ggctagttca
     1501 tgtaatttca tccaattacg attctgaatt agggtttcca ttaagcgtaa ttgtctttga
     1561 acttttgtgg ataaaaggtt tctcatacgg gtatttcctc cataattcat tatatctttt
     1621 tttgtctaag tagtataaaa aaattagagc aagaaaattg tcgttagtaa tcggaaaaat
     1681 tagaataata taagtggctt ttaagtgcaa aaacagaata tattaattgt tcagaaaata
     1741 ttgattaatg cctcaaatag ttgtacattg taaactataa aatactatct aggagtttaa
     1801 aatgaaacat tcacataaaa aatcatttga ctggtatagc atgcaacaac gttattctat
     1861 tcgtaagtat tactttggtg cagctagtgt cttgctcggt accgctttgg tattaggtgc
     1921 agcagctagt gtccaaacag tacaagcgga agaaaacaaa caagaaacta ccaatagtat
     1981 ttctgttggt aggggagaag cagctactaa accagcagag gtttctgcgt ctaataaaga
     2041 gaaaacctat gcagctccaa ctgtagctaa tccagtagaa acgactccag ttaaaactga
     2101 agaagttact aaaccagcag aaaaagttga agaagcaaaa gacaaaaaag aggaagtaac
     2161 gcatcaagat gccgttgaca agtcaaaatt attaacggct ctttcgcgtg ctaaaaaatt
     2221 agaaagcaag ttatatacag aagcaagtgc tgctaacttg caaacaagta tccaagctgg
     2281 tcaaagcttg cttggaaaag cagatgcaac tgaagctgaa ttatcagcag cagagtcatc
     2341 tattcaatca tttattattg gtctagaact tcgttctaac tctaataaag aaactgtatc
     2401 agaaacgcct gtagcgaaga aagctgatgc agttgaatca aaagaagggg ctaaaccagc
     2461 tgcaacaact gaacgttcag ctgttgatag cgctattttg ccaactagca cagctgacaa
     2521 agtagaaaca acttcagctc cagcatctat taatgaaatc ttgaaactag gtttgagcct
     2581 ttctgatgct cgccaaaatc cagctatccg taaggaagat gttaatagag ggtatagtgg
     2641 ttttagagcg gctagcaatc cagccaaccc aattgtctca ggttctggaa atacagttgc
     2701 atttgcagat attagccaag gtggtcgtag ctatagtttc cgtggctacg ggaactcacg
     2761 tggtggaaat tctattcatt acgatgtaac aacagtacgt agtggtaata gtgttaactt
     2821 tacaattagt tattctgcgc caggagattc tagagagttt gttaataata attttatctt
     2881 ggataaaggg gatggatttg gaaatccttc aaatgcaacg atcacaagtt caaatccaat
     2941 agtaagggag caatcaaaat ctattagtca gggtgccaac tacgtatctc actcaggata
     3001 cagtatgacc tctgctattt caacaaatac ggaacaaact atcagattta gtttgcctat
     3061 cattaatcta aatggtgatt tgtctgttcg tttgaaacct gttactttta acgtggacca
     3121 aggtggtggt ggtgccgcta ctagcaatga cccatacagt aactctaact attattatag
     3181 agcaaaccca ctatacttgg atgcaaaccc ttatggtggt actaataata agactgtttc
     3241 agaagacatt gacttccaaa ctgtcgatct tccaactagt aagttaccag aaggtcaaac
     3301 tagattagtt cgagaaggtg aaaaaggaca acgtcaaatt acctataaag ttcatcgatt
     3361 tggtaacgaa acacttttag gattgccgat tagtaatagt gttactaaag aagctaagcc
     3421 acgtattatg caaattggtg tggctaaaga tctaatcgat acagtaaaac cacgtgttga
     3481 tcaaaataaa gtcggtgata caaataacct cactttctat cttgataacg atggaaacgg
     3541 tgtttatact gaaggtgtag acgaacttgt tcaaaaaatt gctattaaag atggagctaa
     3601 aggtgaaaaa ggagcccaag gtgaacgcgg tctaactgga gctaaaggtg aaaaaggaga
     3661 ccgaggcgaa cgcggtctaa ctggagctca aggagctaaa ggtgaaaaag gagcccaagg
     3721 tgaacgcggt ctaaccggag cacaaggtgc caaaggtgaa aaaggggacc aaggtgaacg
     3781 cggtctaacc ggagcacaag gtgctaaagg tgaaaaagga gcccaaggtg aacgcggtct
     3841 aactggaact caaggagcta aaggtgaaaa aggagaccga ggcgaacgcg gtctgactgg
     3901 aactcaagga gctaaaggtg aaaaaggaga ccgaggcgaa cgcggtctga ctggagccca
     3961 aggtgctaaa ggtgaaaaag gagcccgagg cgaacgcggt ctgactggag cccaaggtgc
     4021 taaaggtgaa aaaggagccc aaggtgaacg cggtctaacc ggagcccaag gtgctaaagg
     4081 tgaaaaaggg gaccaaggcg aacgcggtct aactggagct caaggtgaaa aaggagaccg
     4141 aggcgaacgc ggtttaactg gagctaaagg tgaaaaaggg gaccaaggcg aacgtggtat
     4201 cactggagct aaaggtgaaa aaggagccca aggtgaacgc ggtttaactg gagctaaagg
     4261 tgaaaaaggg gaccaaggtg aacgcggtct gactggagcc caaggtgaaa aaggagaccg
     4321 aggcgaacgt ggtttaactg gagctaaagg tgaaaaagga gcccaaggtc aggcaggtcg
     4381 tgacggtgta actccaaccg taaccgttaa agataataaa aatgacggca ctcatactat
     4441 cactattaac gacggtagag gtaatgttac aagtactgtt gtaagagatg gtttcgatgg
     4501 cgcaagtcca ttagttgcga ctcaacgaaa tgacgcagat aagacaacaa ctgttatctt
     4561 ctattacgat aaaaatggaa acaatgaatt agatgcttct gataagaaat taaaagaagt
     4621 tgtaattgca gatggtgcta aaggtgaaaa aggggacaaa ggtgaacaag gtcttcaagg
     4681 gcgtgatggt gaacaaggac caaaaggaga agatgggaaa accccaacag ttaaagtaac
     4741 tgatggtcaa gatggaacgc atacaattac aattaacgat ggtaaaggtg gtataactac
     4801 tacagtagta agagatggat ttgatggtgc aagccctctt gtttctactc atagaaatga
     4861 agcagataaa acaacaactg ttattttcta ttatgatcta aatgataata atcaatttga
     4921 tgaaggagat acaaaactta aagaagttgt tatcgcagat ggaaaacaag gaccaaaagg
     4981 tgacaaaggt gataacggaa aagatggatt cacaccagaa gtaacagtta cagataacaa
     5041 taacggaaca cacaccatca caatcacaca accagacaac agaccatcat taacaacaat
     5101 cgttaaaaac ggtgaagatg gaaaaacacc aaaagtcaaa gcagaacgag atgatgcgaa
     5161 gaaacaaaca acattaacat tctacattga caaagatgga gatggaagtt acacagcagg
     5221 aaaagacgag ttagttcaaa caacagtagt taaagacgga caagacggag ctgcaggagc
     5281 atctggacgt gatggaaaag aagtattaaa cggaaaagta gacccaacaa cagaaggtaa
     5341 agatggagac acattcgtaa atacacaaac aggagatgta ttcgttaaga aaggtaacac
     5401 ttgggaacca gcaggaaaca tcaaaggacc gaaaggtgac aaaggtgcag atggtgccaa
     5461 gggtgaaaaa ggagcccaag gagaacgcgg cctgactgga gcgcaaggtg tcaagggtga
     5521 aaagggagat cagggtgaac gcggtctaac tggatctaaa ggtgaaaaag gagcccaagg
     5581 tgaacgcggt ttaactggag cgcaaggtgc caaaggtgac aaaggtgaac aaggtcttca
     5641 aggtcgtgat ggagctcaag gaccaaaagg cgcagatgga caaagaggac cagcgggacc
     5701 acaaggacca aaaggagaac aaggtaatcc aggaactcca ggtaaagatg gaaaatctct
     5761 aattgctgtt aaaaatggaa ctgaaacaaa agtctatgta gaggatccgg ctagaccagg
     5821 acaaccactt aatccaaacc aaccattagc aactatcact gatggaagaa acggtactaa
     5881 tggacaatca ccaacaatta cagcgacacg aagtgtgcaa aatggcaaaa atggtgtatt
     5941 agtaacaatt acaccagtag gaggtcgtcc acaaacaaca tttgtagagg atggacaaaa
     6001 gggtgctgat gggaaaaccc caacagtaac aataactgag gggcaaaacg gcacacatac
     6061 attaacggtt cataatccag gaagtccaga tgtgacaact acgatccgtg atggagctac
     6121 aggacaagca ggtcgtgatg gtaaagatgt attaaacgga aaagtaaatc cacaaccaaa
     6181 ccaaggtaaa aatggagata aatatattaa taccgaaacc ggtgatgtct atgttaaaaa
     6241 caatggaaac tgggataaag aaggcaacat caaaggccct aaaggggaca aaggtgcaga
     6301 tggtgctaaa ggcgaaaaag gagaccaagg cgaacgcggc ctaactggag cgcaaggagc
     6361 taaaggtgcg gatggcgcag taggtcgtga tggacgtgac ggtaaagacg tgttgaacgg
     6421 caaagctaac ccagaagcac atcaaggtaa agacggcgat aaatacgtta atacagaaac
     6481 aggcgacgtc ttcgttaaga ataacggcaa ctgggataaa gagggcaaca tcaaaggccc
     6541 taagggtgac aaaggtgaac gcggagaaga tggtaagact ccagaagtaa ctgtaactcc
     6601 aggtaaagat ggccatagta ctgacattac attcactgtt ccaggtaaag atccagttac
     6661 agtaaatgtt aaggacggag aaaatggtct gaacggtaaa actccaaaag ttgatttact
     6721 tcgtgtccaa ggaaaaaacg gaaatccatc tcatacaatt gtgacattct atacagatga
     6781 aaacaatgac ggcaaatata caccaggaac tgatgaactt ctaggttcag aaatgattaa
     6841 agatggtgct aaaggcgcgg acggacgaga tggtaaatca ttgcttactg tcaaggatgg
     6901 taaagaaact aaagtttacc aagaagatcc agctaaccca ggacaaccat taaatccaga
     6961 aaaaccactt gcggtaatta gagatggagt agatggaaaa tcacctacag ttacagctgt
     7021 tcgtaaagat gaagcagggc ataaaggtgt agaaatcact gttgataacc atgatggttc
     7081 acaaccaact acagtctttg ttcaagatgg tgctaaggga gaaactggtg caaccggtca
     7141 ggatggacaa actcctacaa tcactactca acgtggacaa gatggccaaa gcactgttgt
     7201 aactatcaca acatcaggta aagatccagt aaccttcact gtaaaagatg gtaagaatgg
     7261 taaagatggc cgtgcaccga aaatcaaagt agaagatatt acttcacctt caagaattag
     7321 acgcgataca gatgctgctg caactccaac gcgtaacggt atccgtgtta cagtttatga
     7381 tgatgttaat gacaatgggg tatacgacga aggtgtcgat aaagtattaa atagtaaaga
     7441 tatttataac ggtatagatg gacgtgatgg ttcagctcca actattacta caaaagataa
     7501 tggagatgga actcacacta tcacagttca aaacccagat ggttctgaat caacaacagt
     7561 tgttaaagat ggtaaagacg gtaaaactgc gaatatcact acaacagaaa acccagatgg
     7621 aagccacaca attacggtga caaatccaga tggttcaact aaagaaactg ttgttaaaaa
     7681 cggtaaagac ggtaagactc ctaaagttga agtaacggat aacaacgatg gaactcatac
     7741 tgttaaagtt acagatggag acggcaatgt taccaacgct atcatcaaag atggtaaaga
     7801 cggtaaagct gcaacagcaa caactactga aaatccagat ggaagccaca cagtaacaat
     7861 cactaaccca gacggaacta agaatgagtt tgttgttaag aatggacgtg acggtgttga
     7921 cggacgtact ccaaccgcat ctgttcgtga taatggagac ggaagtcata caatcgttat
     7981 tacaaatcca gaaggtgtga caactgaaac cacagttcgt gatggtaaat caccaaaagt
     8041 gactataact gatgaacaaa atggaactca taagatctct gttctaaatg gtgacggaac
     8101 aactacaaca actacagttc gtgatggtag agaaccaaaa cttgaagtta ttgataacaa
     8161 cgatggttca cacactatta aagtgacagg tgctgatggt aaaggaacga caactacaat
     8221 ctttgatggt aaatcaccaa aagcgaacat cgttgataac ggagatggaa ctcatacatt
     8281 aacaatcgta gattctgatg gtcgtgaata caaatctatt atcaaagatg gtaaagacgg
     8341 caaagatagc gtttcaccaa ctgtaactgt taaaaataat aacgatggaa ctcacgttgt
     8401 tacaatcact aatccagatg gaagtaagac agaaatggtg attaaagacg gtaaagatgg
     8461 taaatcacca aaagtttctg ttgaagataa tggtgatggt agtcatacaa tcacaatcat
     8521 caattctgat ggaactgtga caaaaacagt tattaaagat ggtagagatg gacgtgatgg
     8581 tcgagacggc aaagacggta aagacggtaa agatggaaaa tgtggatgcc aagacaaacc
     8641 agtaacacca tcaaatgaca aaccagttcc tccaacacca aatgtgccga caccagaagt
     8701 accggttaaa cctgtgccag cggttccaga acaaccagta gtaccaacac cggctcaacc
     8761 agcaactcca gtaaatgcta acccagtagc accaactaca ggtaaagaaa accgtgggga
     8821 caaattacct gaaactggaa gccaatctga ttatatctct gttcttttag gtagcggtat
     8881 tctattgagc ctatatgtag gacgaagaaa agaagcttaa tcagccaaaa gcagtcacta
     8941 atttctcgaa agaagatgat aagtgataga tctcgaatga aagaatattc ttagaatgat
     9001 tttcctatga tggaaaagac tgctaaaaac cttgagtggt aacgctcaag gtttttttgg
     9061 atggctgatg tttgtctgaa aatcttgatt gagaaataaa aagccaccct attaggtggc
     9121 tataattgat ttagactttc taaaattttc tattttttac tgaatcgctt cgaccaagtc
     9181 ttgagcttgt ttttcaagtg agtttaggac tgtttcttca agaaccaatt ttccgtctgc
     9241 ccaggcagag tcattaacac gtgcagcagt gaaatcacca acgccttgtg tacggataaa
     9301 tggcaagagg tctttgtaga tagcgaaaag ttgatcgtgc cctgcattgg ctacagatga
     9361 tactgtgaca aacttgtctt gaagggcaga aacgccacgt gtatcagaca agtcaagggc
     9421 acgagatagc cagtcaagca agtttttcac tgtaccaggg atagagaagt tgtagactgg
     9481 agagaaaatc cagatagcat ccgcaacgag aactgcttca cgagcagcag ctacagctgg
     9541 atgtgttgga acttccaaat cttggctgaa gagaggaagg gctgaataat caaggtagct
     9601 aacttccgct ttaccagcaa gtgctttctc agcttcgagc gccatttggt ggttgaaaga
     9661 accttgacgt agtgatccga cgataaataa tactttttta gacatgatgt gtctcctttt
     9721 tcttaaaatt tcgagaactc tctcgttgtt ataaaatata ttacaacaaa acaaaagaac
     9781 ttgcaagaaa tttgctcaga gtttatttga aaattgtaaa atttcggaaa ctctaatcat
     9841 ttgtgactga aaaagtaaga actgtttcac tttcaaaatc ggttttgttt gaagggagaa
     9901 atagaattgg ttgctcaaag cacagctttg aggttgcaga taaagctgac gtggtttgaa
     9961 gagattttcg aagagtattc ttatattcac ttgtaataaa gcttaataga tggtaaaata
    10021 gacgagtgaa aaaaagacaa aacaaagcaa aaaataatct actgtggcag tatggtctag
    10081 ggatgacgat tttgtttgtg gttatcagtg gttcctttct gtatatggtt tctcttagca
    10141 tgaaacccta tcaaacagct aaaagtgaag gagaaaaatt agctcagcag tatgcaggat
    10201 tagagcaggc tgatcaggtt gatttataca atggcttgga atcttattac agcgttcttg
    10261 gtcgtaacaa acagcaagaa gcgcttgctg tcctgattgg taaagatgac cataagattt
    10321 acgtttatca gctaaatcag ggtgtttcac aagaaaaagc agaaacggtt tctaaggaaa
    10381 agggagctgg cgagattgac aagataacct ttggccgtta tcaagataag ccaatctggg
    10441 aagttaagtc aggatctgat ttttatctag tagattttga aacaggagca ttggtcaaca
    10501 aggagggcct atgaaactat ccaaccgtgt tttagaaatg gaagaaagtg tgactttggc
    10561 tgctggagcc cgtgccaaag cactgaaggc tgagggtaga gatattttgt ctctaacctt
    10621 gggtgagcca gatttcacta ctcccaaaaa tatccaagat gccgccattg catcgattcg
    10681 agatgggcgt gcttcttttt atacagtaac ctcaggtctg ccagagctta aggcggcggt
    10741 caatagctac tttgagcgct tttacggcta ttctgtagcg tcaaatcaag tgacagtcgc
    10801 tgcgggagcc aaatattctc tctatacctt ctttatggct gtggtcaatc caggtgatga
    10861 agtcatcatc ccaaccccat actgggttag ctatggagat caggtcaaga tggcagaagg
    10921 cgttcccgtc tttgtttctg ctaaggaaga caatcacttt aaggtgaccg tagagcagtt
    10981 agaagcagct cgcactgaca agaccaaggt tttggtgctg aattcgccat ctaatcccac
    11041 aggtatgatt tacacccgtg aggaactctt ggcaattgga aactgggctg tagaaaatga
    11101 tattctcatc ctagcagacg atatctatgg ccgcttggtt tataatggtc atgagttcac
    11161 accgatttct agcctatcgg aagcgattcg caagcaaaca gtggtcatca atggtgtgtc
    11221 taaaacttat gccatgactg gttggcggat tggttatgcc gttggagaag cagacattat
    11281 cgctgccatg tccaagattg caggtcaaac aacttcgaat ccgtcagcag tagcccaata
    11341 tgcagcagtt gaggctctat caggtgagca agatactgta gaaagcatgc gtcaggcctt
    11401 tgaggaacgt cttaatacca tctatcccct ccttgcagag gtaccaggat ttgaagtggt
    11461 caaaccgcaa ggggccttct atctcttccc aaatgtcaaa aaggccatgg agatgaaagg
    11521 ctacacggat gtgacagact ttacaactgt tatcttagaa gaagccgaag tggccttggt
    11581 aacaggagct ggatttggag caccagaaaa tgtccgcctc agctatgcga cagacctaga
    11641 cacgcttaaa gaagcagtcg aacgcttgaa agcatttatg ggtagtgaga atgattgatc
    11701 attttgagat taaggtaaag gatttacaaa tttcagaagg attttatagg agttttctcg
    11761 ctcctttgga ctataaattg gcttttaaga ctagttctct aattagtttt ctatccccga
    11821 acagccctca tcctggtggt gatttttggc tgactcaggg aacacaagat cctgttcatt
    11881 ttgctttttt agcagaaaat aaggaagaag ttcaggcttg ttatgaggct ggcttagagg
    11941 caggtgggcg agacaatggg gctcctggtt atcgaagtga gcatccgatt tactatgctg
    12001 cttttatgat tgacctggat gggaacaata tagaggtggt ttgccataaa gaataaaaaa
    12061 gaaaaggaaa aataatgaca aaacgtgtaa cgattattga cgtaaaagac tatgttggtc
    12121 aggaagtgac gattggcgct tgggttgcca acaaatcagg aaaaggaaaa atcgctttct
    12181 tacaattgcg tgatggaaca gccttctttc aaggtgtggc ttttaaacca aactttgtcg
    12241 aaaaatttgg tgaagaagtg ggacttgaga agtttgatgt tatcaaacgc ttgagccaag
    12301 aaacgtctgt ttatgtgaca ggtattgtca aagaggacga acgttctaaa tttggctatg
    12361 agttggacat cacagacatc gaagtgatcg gtgaatctca agactaccca atcacaccaa
    12421 aagaacacgg aacagacttt ttgatggata accgtcactt gtggctacgc tctcgtaagc
    12481 aagtagctgt gttgcaaatc cgtaacgcta ttatctatgc aacttatgag ttctttgaca
    12541 agaacggttt tatgaagttt gacagcccaa ttctttcagg aaatgcggca gaagattcta
    12601 cagaactctt tgaaactgac tacttcggaa cgccagccta cttgagccaa tcaggtcagc
    12661 tttacctaga agcaggggct atggctcttg gtcgtgtctt tgactttggt ccagttttcc
    12721 gtgctgaaaa atcaaaaaca cgccgtcact tgactgagtt ctggatgatg gatgctgagt
    12781 actcatactt gacacatgat gagtcgcttg acttgcaaga agcttatgtg aaagctcttc
    12841 tacaaggcgt tcttgaccgc gcgcctcaag ccttggaaac cttggaacgt gatacagaac
    12901 tcttgaaacg ctacattgca gagccattca aacgtatcac ttacgatcaa gccattgacc
    12961 tcttgcaaga gcatgaaaat gatgaagatg ctgactacga gcatcttgag catggtgatg
    13021 actttgggtc accacacgaa acttggattt caaaccactt tggtgtgcca acatttgtca
    13081 tgaactatcc agcagccatc aaggccttct acatgaaacc agttcctgga aatccagagc
    13141 gcgtgctttg tgcagacttg cttgctccag aaggctatgg agaaattatc ggtgggtcta
    13201 tgcgtgagga agattacgat gcccttgtcg ctaagatgga tgaacttggc atggatcgta
    13261 cagaatatga attctacctt gaccttcgta aatacggtac agttccacac ggaggatttg
    13321 gtatcggtat cgaacgtatg gtaaccttcg cagcaggaac aaaacatatc cgtgaagcta
    13381 ttccattccc acgtatgttg caccgtatca aaccataaga acaggaaaac gcccatgtgg
    13441 cgttttttcg aattctagtg tttttacttg cgtaaaaaaa ttttttctag tataatagtt
    13501 tattgtgagc gaacctcact taccccttgc aaagtcttgg ggtcattaga ccaaaaggag
    13561 gaacatatca atggctaaat acgaaattct ttatatcatt cgtccaaaca ttgaagaaga
    13621 agctaaaaac gctttggtag cacgttttga ctctattttg actgacaacg gtgcaactgt
    13681 tgttgaatca aaaacttggg aaaaacgtcg tcttgcatac gaaatccaag atttccgtga
    13741 aggactttac cacatcgtta acgttgaagc aaatgacgat gcagctctta aagagtttga
    13801 ccgtctttca aaaatcaacg ctgacattct tcgtcacatg atcgtcaaaa ttgacgcgta
    13861 agaaggtaaa ctatgattaa caatgttgta cttgtagggc gtatgacacg tgacgctgag
    13921 ttgcgttata ccccatcaaa tgtagcagtt gcgactttta ctcttgcagt aaaccgtaca
    13981 tttaagagtc aaaatggtga acgtgaggct gattttatca atgtcgttat gtggcgccaa
    14041 caggctgaaa atcttgctaa ctgggctaaa aaaggctcac ttatcggggt gacaggtcgt
    14101 atccagactc gtagttacga taaccagcaa ggacaacgtg tctacgtgac agaggtcgtg
    14161 gctgagaatt tccaaatgtt ggaaagccgt agtgtgcgtg agggtcacac aggtggagct
    14221 tactctgcac caactgcaaa ctattcagca cctacaaatt cagtaccaga cttttcacgt
    14281 aatgaaaatc catttggagc aacaaaccca ttggatattt cagatgatga tttaccattc
    14341 taatggacaa ctaaacttat aaaggagaaa aaacatggct caacaacgtc gtggcggatt
    14401 caaacgccgt aaaaaagttg attacatcgc agcaaacaaa attgaatatg ttgattacaa
    14461 agatactgag cttcttagcc gtttcgtttc agaacgtggg aaaatccttc ctcgtcgtgt
    14521 aacaggaact tcagctaaaa accaacgtaa agtaacaaca gctatcaaac gcgctcgcgt
    14581 aatggctttg atgcctttcg taaacgaaga ttaaagaaaa gacccagttg ggtctctttt
    14641 tcatgagctt gaaaacaaga aagcgaatta agaccccaac gggtcttttt tcacaagctt
    14701 tgaaatgaga gagcgagtta ggctcagtgg ctcccttttt acgagtttga aaacaagaga
    14761 acacattaaa agcacgatgg agctttttca taaatatgtc tcttttctca tccaagaaat
    14821 taaaataaaa atgataaact ttttgaaaat aggggatttg cctactttat gatataatgg
    14881 aaagagttag attgaaagag gtagtgagat ggacgcgaaa ttaagataca aggcaaagaa
    14941 gatcaagatt gtcttttttg atattgatga tacattgcgg aattcaaaga cagggtttat
    15001 tccaactaca attcccactg tttttaaaca gttgcgtgaa aaaggaattt taacaggaat
    15061 cgcctctgga cgtggcattt ttggtgttgt tccagagatt cgtgatctca agcctgactt
    15121 ttttgtaact ttgaatgggg cttatatcga agataaaaaa ggtcaggtca tttatcagca
    15181 tcagattgaa aagtcagatg ttgaggagta tatctcttgg gctaagcaag aaggaattga
    15241 gtatggcttg gttgggagtc atgatgccaa gttgtcgact cgcaccgata tgatgagtga
    15301 agctatcaat ccaatttatc ccgacttaga tgtagatccc gatttccatg aaaaagaaga
    15361 tatctatcag atgtggactt ttgaagataa gggagatgac ttgcacttgc ctgacagtct
    15421 ctcagacaaa cttcgcatgg ttcgttggca tcaacattcg tctgatattg tgccgatttc
    15481 aggctccaaa gcgacggggg tggaaaaggt tgtggaacac cttggcttga aaccagagaa
    15541 ggtcatggtt tttggagatg gtctcaacga cttggaactc tttgattatg ctggaatcag
    15601 cgttgccatg ggaatttctc atgataaaat caaagaaaaa gcagattata ttacaaaaac
    15661 attagaagaa gatggcattt ttgctgcctt agaagtattt ggtatggtag aaaaagaatt
    15721 gcattttcca caagtagaca ttgaaacagt agaaggtcct cttgcgacta ttaagaccaa
    15781 tcacggagac ttacgtatca agcttttccc tgaacatgct cctaaaacag tggctaactt
    15841 tgtatatctt tcaaaagatg gctactatga tggtgtcatt ttccaccgta ttatcaagga
    15901 ctttatgatc caaggtggag acccaactgg aactggtatg ggtggcgagt caatctacgg
    15961 cgaatctttt gaggatgaat tctcagaaga gctttacaat atccgtggtg ctctttccat
    16021 ggcaaatgct ggtccaaata ccaacggcag ccagttcttt atcgtgcaaa accaacacct
    16081 accttattct aagaaagaaa ttactcgtgg tggttggcca gaaccgattg cagaaatcta
    16141 tgccaatcaa ggtgggacac ctcacctaga ccgccgtcac acggtttttg gtcagttagc
    16201 tgatgaagca tcttacgctg tcttggatgc cattgctgct gttgagacag gagctatgga
    16261 caagccagtt gaagatgttg taattgaaac tattgaaatc gaggactaag atgaaaatcg
    16321 gtgataagct aaaggggcgt attacaggga ttcagcccta cggtgccttt gttgagttag
    16381 agacgggtga tacggggctg attcacattt cagagattcg gacaggattt attgaaaata
    16441 ttcatgaaac cttgaaagtc gatgaagagg ttcaagttca ggtagtggat ttagatgaat
    16501 ttacagggaa agccagtctt tctatccgca ctttggagga agaaaagtac cagtttccta
    16561 gacggagacg cttttcaagc gaccgcttta actacggctt tgctcccttt cgacgaatgc
    16621 taccaatctg gactggagaa gccctgcacc atttaaaaaa gaagaagtag tttagagttt
    16681 tatatttttt gtaatgttaa tataaatttg ctataatagg accatggaag aagaacaatt
    16741 attaaaatca ggagagcgca ttaaccagct cttttcgaca gatatcaaaa tcattcaaaa
    16801 tagagaggtt tttagttatt cggtggatag tgttctctta tcacgatttc cacgttttcc
    16861 taagaagggg ttgattgtgg atttttgtgc tgggaatgga gcagtggggc tttttgctag
    16921 cactcgtact caagcacaga tattgtctgt tgagattcag gagcgtttgg cggatatggc
    16981 tgaacgctct gtccgtttga atggattaga ggaacaaatg caggtcatct gcgatgattt
    17041 gaaaaatatg cctgctcaca ttcaggggag taaggtggat atgattttgt gtaatccacc
    17101 ctatttcaag gtggatcctc attctaatct gaacgagagt gaacattatc tcttggctcg
    17161 acacgaaatc acgactaatt tggaagaaat ctgtcatagt gcccagagta ttctcaagtc
    17221 taatgggcgt ttggccatgg ttcatcgtcc tgatcgactt ctggatattt tggatacgtt
    17281 aaaacgacat aatctagccc ctaagcgcct gcagtttgtt tatccaaaaa gagaaaagga
    17341 agccaatatg cttttgattg aggcgatcaa ggatggctca acaagtggct ttaaggtctt
    17401 acctcctttg attgtccaca atgatgatgg ctcttatacg cccgaaatcg aagagattta
    17461 ttatggatca taaggcttat atgtacgttc tggagtgtcg tgatggatcc tactatatag
    17521 gctatacgac tgatgtgaga agacgcctcg ctatccacaa tagtgggaag ggagccaaat
    17581 atacacgagc acgcttgcca gtcaaactta tctatgctca aggttttgcc agtaaggaag
    17641 aagccatgtc ggctgaagct cttctcaagc gtaagaagag gccacagaag gaagaatttt
    17701 tatctgaaaa tcaagataga aatttactca gttattttga agaatcgtgg ggagtccttt
    17761 gactcctctt acctttgtca aaaagcgaaa aaaatgctac aataaagaaa ataaacaaaa
    17821 tgaggtatta tcatgtccaa gattctagta tttggtcacc aaaatccaga ctcagatgcc
    17881 atcggatcat ctgtagcttt tgcctacctt gcaaaagaag cttacggttt ggatacggaa
    17941 gctgttgccc ttggaactcc aaatgaagaa acagcctttg tcttgaacta ttttggtgtg
    18001 gaagcaccac gtgttatcac ttctgccaaa gcagaggggg cagagcaagt tatcttgact
    18061 gaccacaatg aattccaaca atctgtatca gatatcgctg aagtagaagt ttacggtgtt
    18121 gtagaccacc accgtgtggc taactttgaa actgcaagcc cactttacat gcgtttggag
    18181 ccagttggat cagcgtcttc aatcgtttac cgtatgttca aagaacatgg tgtagctgtg
    18241 cctaaagaga ttgcaggttt gatgctttca ggtttgattt cagataccct tcttttgaaa
    18301 tcaccaacaa cacacccaac agataaaatc attgctcctg aattggctga attggctggt
    18361 gtgaacttgg aagaatatgg tttggcaatg ttgaaagctg gtaccaactt ggctagcaaa
    18421 tctgctgaag aattgattga catcgatgct aagacttttg aactcaacgg aaataatgtc
    18481 cgtgttgccc aagtgaacac agttgacatc gctgaagttt tggaacgcca agcagaaatt
    18541 gaagctgcaa tgcaagctgc caacgaatca aacggctact ctgactttgt cttgatgatt
    18601 acagatatcg tcaactcaaa ctcagaaata ttggctcttg gtgccaatat ggacaaggtc
    18661 gaagcggctt tcaatttcaa acttgaaaac aatcatgcct tccttgctgg tgccgtttca
    18721 cgtaagaaac aagtggtacc tcaattaact gaaagcttta atgcgtaaga ttttgggtgt
    18781 cagctcaaaa tcggaaagtc tagtttgcct tatatcgaaa ggagtttcgg ctcctttttt
    18841 tctaggagtg aagtatgtta gaaaatggcg atttgatttt tgtgagagat gggtcagaca
    18901 tgggacaggc catccagact tccacaggta actatagcca tgttgccatt tatttggatg
    18961 ggatgattta tcatgctagt ggacaggctg gtgttgtctg tcaagaaccg gcagacttct
    19021 ttgagtccaa tcatttgtac gacctctatg tttacccaga aatggatatc cagtcggtga
    19081 aggaaagagc ttgcagacat cttggagcac cctacaatgc ttctttctat ccagatgcag
    19141 ctggtttcta ctgctcccag tatatagcag aaatcctacc tatttttgaa actattccta
    19201 tgaaatttgg agatggggaa caggagatta gtgatttttg gagggagtat tacatagaac
    19261 taggtctgcc tgttcctctg aaccaagctg ggaccaatcc tagtcagttg gcagcatcgc
    19321 ctctgttaca atgtaaagaa aggaatcttc atgattcaga tttttaatcc atctcgtttg
    19381 acgagacagc catttttgga gaattgatcc gctatctgga ccagtatgag gatgtgattc
    19441 tacgggaaat taaggctcaa tttccagatg ttgcagttga taaactcatg gaagagtata
    19501 taaaggcaag cttgattcta cgtgaaaata agcgctatta cctcaatttt cctacgcttg
    19561 aatcacttga tagtcttgaa ctggatcaag agatttttgt cagagaagct agtccggtct
    19621 atcaagcctt gttggagtag agttttgaga cggaattgcg caatcaaatc aatgcagcta
    19681 ttttagttga aagacggact ttgcgcgcat taaaatgacc ctgtccaatt atttttacaa
    19741 ggtcaaacag cagtatcctt tgacagaaaa acagcaggag ctctatgaca ttttaggaga
    19801 tgttaatcct gagtatgcac tcaagtatat gacggctttt ttgttgaaat ttctcaaaaa
    19861 agaccagctt atgcagaaat gccgtgatat ctttgtggac agtttggttg tcttaggcta
    19921 tattgtgcaa aatgaagata gaaagtatga gttggctatc gattttgata aggagagatt
    19981 aactttctac ttagcgtgat ttcttgtttc tgagcacatt gtttgacttt ccttagtatt
    20041 cggtataaac tatatgtaac cggtaacaca tatcggaata aactaaagga gacaatcata
    20101 tgtcacttga aaacaaattg gaacaagcaa caggcgctgt caaagaaggt tttggtaaag
    20161 ttactggaga cagcaagaca gaacttgaag gagctgttga aaaaacagtt gctaaggcaa
    20221 aagacgttgt agaagacgca aaaggtgctg tagaaggtgc cgttgaaggt ttgaaaaacg
    20281 tttttactaa ataataggaa aaaatcaagg gtttcatttt cccttgattt tttctattct
    20341 tataaataat tttctgcgac ggctgtatct cctgggtagg attctttctt gccctggatg
    20401 atttggtaac aatcggctcc cttacccgca ataataactg catctaattc gtgatttgtg
    20461 atagccattg ccgccttgat ggcttcttgg cgatccgcaa tcttttcaac aggatgattg
    20521 atgtagctac taatttcatc tgcaatggcc attgggtctt catagttagg gtcatcagca
    20581 gtcagaaaga cttgaatctc agggtgttga ttgaggagga ggccgaagtc cttacgacga
    20641 ctttctccct tgtttcctgt tgttcccaga accagagcaa tctttccggt ttgatgagtt
    20701 tcaaccacat tgatgagttt tttcagacta tccccattgt gggcatagtc gatgaagacc
    20761 ttggctccat ttttctgagt gaggacttcc atacgaccag gaacgcgggt tgcagcgatg
    20821 ccttttttga tgtcctcaag acttgctccg agacggagac aagcaagtcc agcagcaact
    20881 gcattttctt ggttgaagtt gccaatgagt tggatatcat aatctccagc gagtttaccc
    20941 gtagctgaaa agctaaaggc tttggaattc tcgatttggt tatcaaattg gctaccatag
    21001 aaatcatggt cttgatcttc aacctgttct ttcaagactg agaagtggtc catgtcactg
    21061 ttaatgatga ctgctcggct attttccatc aagagacgct tgtggtagaa atagtcttca
    21121 aagctagggt gttcaatcgg gccgatatgg tcaggagtga tgttaagaaa gactcctaca
    21181 tcaaaggtca gtccatagac tcgatggact agataggctt gactggagac ttccatgatg
    21241 aggtgggtac ggtcattttg cacagcctga ttcatcatgt caaagaggtc aatactctca
    21301 ggggttgtca acgctgactt aaagaaagtc tcgccatcaa gagttgtgtt catggtcgac
    21361 aacatagcag gtctatgccc ttgagataag atgttatagg cgaaataggt tgctgttgtc
    21421 ttacccttag taccagtaaa ggcaaggagt ttgagtttct cttgtggatt accatagaac
    21481 tccatggcaa tcaaactcat ggctttcttt atatcgttca caatgatgac agggataccg
    21541 acttcgtagt ccttttcagc tacataccaa gctaaacctt gtgttatagc agaaagaagg
    21601 tattcttttt taaaggcagc gccttttgca aaaaaaagag tgtcttctgt tacttttcgg
    21661 ctgtcgtagc tgatgctatc aaaaataact ttgctgtagt tgtagtggta atgaccttgg
    21721 tcaataattt cgcgaaaaag gccatctttc tttaaaatat ctaatacggt ttcaatctta
    21781 atcatacttt ctattgtaaa ccgaaagtcg taaatttaca agtaacaagg aaaagtttat
    21841 aatggaagat aaggagtttt tcctagttat caaaattgaa tgaggaatct atgtcgcacg
    21901 aaaacaatca ccagcaggcc cagatgttac gggggactgc ttggctaacg gctagtaact
    21961 ttatcagtcg cctactcggg gctgtttaca ttatcccttg gtacatctgg atgggggctt
    22021 atgcagctaa ggcaaatggt ctctttacca tgggttacaa tatctatgct tggttcttgt
    22081 tggtttcaac agcggggatt ccagttgcgg tggccaagca agttgccaag tataatacca
    22141 tgcgagaaga agagcatagc tttgccctga ttcggagctt cttaggcttt atgacaggac
    22201 taggcctggt ttttgcttta gtcttgtatg tctttgctcc ttggctagca gacttgtctg
    22261 gcgtgggcaa agacttgatc ccaatcatgc aaagcttggc ttggggagtc ttgattttcc
    22321 cgtctatgag tgttatccga ggatttttcc aagggatgaa taacctcaaa ccctatgcca
    22381 tgagccaaat tgctgagcag gtcattcgtg ttatctggat gctcctagca acctttatca
    22441 ttatgaagct cggttcagga gattatctag cagccgttac ccaatcaacc tttgctgcct
    22501 ttgtcggtat ggtagccagt tttgcagtct tgatttattt ccttgcccaa gaaggttcac
    22561 tcaaaagagt ctttgaaaca ggagataaga ttaacagtaa gcgtctcttg gttgatacca
    22621 ttaaggaagc cattcctttt atcctgacag ggtctgccat ccagctcttc cagattttgg
    22681 atcagctgac ctttatcaat agtatgagct ggtttaccaa ctacagcaat gaggacttgg
    22741 ttgtcatgtt ttcttatttc tcagccaatc ctaataaaat cacgatgatt ttgatttctg
    22801 taggggtttc gattgggagt gttggtttgc cacttttgac ggaaaactat gtcaaggggg
    22861 acttgaaagc agcttctcgt ctggttcagg acagtctcac cctactcttt atgttcttgc
    22921 taccagcaac ggttggagtg gttatggtag gagaacctct ttatacggtc ttctatggta
    22981 agccagatag tttggctatg ggcttatttg tctttgcagt tttgcagtct attattttag
    23041 gtttgtacat ggtcttgtct ccaatgcttc aggccatgtt ccgcaaccgc aaggccgttc
    23101 tctattttat ctatggttct attgccaagc tagtcttgca actacctacc atcgccctct
    23161 tccacagtta tggtcctttg atttcaacaa ccattgctct catcattcct aacgtcttga
    23221 tgtatcggga tatttgtaaa gtaactggtg tcaagcgcaa ggtgattttg aagcgaacca
    23281 ttttaatcag tttgctgacc ctagtcatgt ttctgttaat aggaaccatc cagtggctgt
    23341 taggattttt cttccaacca agtggacgtt tgtggagctt cttttatgta gctcttgtcg
    23401 gtgccatggg gggtggactt tatatggtta tgagtctgcg tacctattta ttagataagg
    23461 taataggaaa agtccaagca gatcgcctgc gagcaaaatt taagctttcg taaggtggca
    23521 tttataaata aaaggaataa gttgaacaat cttttaggag aacttgaaac ttattccttt
    23581 ttttagcgtc aaaatagcta aaattatctt tcaaaaatag aaaatgaggt ttttttcgat
    23641 tttttggaaa aaacaattga ctaaacaaac aaagagttgt ataataaaac aaatatttaa
    23701 agcaggaggt tctggaaatg aaaaagtcta agagcaaata tctaaccttg gcaggtcttg
    23761 tcctgggtac aggagtttta ttgagcgcgt gtggaaattc tagcactgca tcaaaaacct
    23821 ataactatgt ttattcaagt gatccatcta gtttgaacta tctagcagaa aaccgcgcaa
    23881 caaccaatga tattgtgacc aatctggtag atggtctcat ggaaaatgac caatatggga
    23941 actatgtgcc atccttggca gaagattgga ctgtttctca ggatggtttg acctatacct
    24001 acaaacttcg taaggatgcc aagtggttta cttctgaggg agaagaatat gcacctgtaa
    24061 ctgcccagga ttttgtgaca ggattgcaat atgcagctga taaaaaatca gaagccttgt
    24121 atctagtgca ggactctgtt gcaggtttgg atgactatat cactggtaaa acaagcgact
    24181 tttcaactgt cggtatcaag gctcttgatg atcaaaccgt tcaatacacc ttggctcgac
    24241 cagaacctta ctggaactca aaaacaacct caaccattct tttcccagtc aatgcagatt
    24301 tcttgaaatc aaaaggggat gattttggca aggtagaccc tgctaatatt ttgtacagtg
    24361 gacctttctt gatgaaagct tttgtttcaa aatctgtcat cgaatacaag aaaaatccta
    24421 actactggga tgctaaaaat gtctttgtag acgatgtgaa attgacctac tatgatggta
    24481 gcgaccaaga atcactggaa cgtaatttta cagctggtgc ttatactacg gctcgtcttt
    24541 ttcctaacag ctccagctat gaagggatta aagaaaaata caaaaacaat atcatctata
    24601 gtatgcaaaa ttcaacttca tatttcttta attttaacct agataggaag tcttacaatt
    24661 atacttctaa aacaagtgac attgaaaaga aatcgactca ggaagcagtt ctcaataaaa
    24721 atttccgtca ggctatcaat tttgcttttg acagaacatc ttatggggct cagtctgaag
    24781 ggaaagaagg tgcaacaaag attttgcgta acctagtggt tcctccaaac tttgtcagta
    24841 tcaagggaaa agactttggt gaagttgtag cctctaagat ggtcaactat ggtaaggaat
    24901 ggcaaggtat caactttgcg gatggtcaag acccttacta caatcctgag aaagccaagg
    24961 ctaagtttgc ggaagctaag aaagaactcg aagcaaaggg tgttcaattc ccaatccact
    25021 tggataagac tgtggaagta acagataaag taggcataca aggagttagt tctatcaaac
    25081 aatcaattga atctgtttta ggttctgata atgtagtgat tgacattcag caattaacat
    25141 cagatgagtt tgacagttca ggctactttg ctcaaacagc tgctcagaaa gactatgatt
    25201 tatatcatgg cggttgggga cctgattatc aagacccgtc aacctatctc gatattttta
    25261 atactaatag tggaggagtt ctacaaaatc ttggactaga gcctggagaa gccaatgaca
    25321 aggctaaggc agttggactg gatgtctata ctaaaatgtt ggaagaagct aataaagagc
    25381 aagatccagc caaacgttat gagaaatatg ctgatattca agcttggttg attgatagtt
    25441 ctttagttct tccaagtgtt tcgcgtgggg gaacaccatc attgagaaga accgtaccat
    25501 ttgctgctgc ctatggttta accggtacaa aaggggttga atcatataaa tacctcaaag
    25561 tacaagataa gattgtcaca acagacgaat atgcaaaagc cagagaaaaa tggttgaaag
    25621 aaaaagaaga atccaataaa aaagcccaag aagaattggc aaaacatgtc aaataaaatc
    25681 taattgtaga taagtttgtg taagaagtct ttgtgcttaa aaacgcataa tatcaggtat
    25741 ataaaatcct agatgttatg cgttttgtta taggaagatt tattagattt tatcctcaaa
    25801 ttgagttatt cccgacttta tccgttgaaa tattataagg agtatttatg aaattaaaat
    25861 tattaagagt agatactaag gtgattatgg ggagtttctt acttgttctg tctagtctac
    25921 ttgctttgtt gcttcccctt atcttaaagg gtttaataga tgggagttct attgaaaata
    25981 taggttccaa agtatttcaa tcgtttttga tttttattgg tcaagccttg ttttcttcta
    26041 ttggttacta tctgtttagt caatcgggtg aaaaaaagat agcaaaaatc aggaaaaaag
    26101 tgacagaggg gttgatttat gcagagaaat ccttctttga taagagccaa agtggggagt
    26161 tgacttctgc cattgtcaat gacacgagtg tcattcgtga gtttttaatt acgactttcc
    26221 caaatattat tctgagttta gttatggtac ttggttccat tgtagtctta tttagtcttg
    26281 attggaatct ttctctactt ttattcatca ctcttccttg tatgatgttt attatcttgc
    26341 ccctttccaa tatcagtgaa aagtatagtc gtcgtttaca ggaggaaatc ggatttttaa
    26401 cagggcaatt gactgaaaag attcaagaac atgaattaat taagactaac caagcagaga
    26461 aaagtgtaca ggatgttttg gataattgta ttgaaagggt acaaaacaat tcactgaaat
    26521 cagatagggt tacttctttt gagaccccgt ttgctctctt atttatcttt gcgactatag
    26581 ctgtgatgct aacctatggg ggttatcggg tcagcgcagg atatatatct gtgggaacct
    26641 tggtttcgtt tttgatttac ctctttcaat tacttaatcc tattagtaat atagctaatt
    26701 ttgtaactgt ttattctagg agcaagggat cttcagttgc actggagaac ttgcttgcag
    26761 ttcctaaaga aaaatttgag ggaggaaaat cggtatcagg acaagggttg aattttaacc
    26821 atgtctattt tggttatgat gaaaatcgac ctgtcttaaa ggatattact tgttcaattt
    26881 tcaaggggca aaaaattgct tttgttggac catctggatc aggaaaatca acgattgtgc
    26941 gtttgttaga gcagttttat aaaccgcttt caggagatat tctaatggag caatcaagta
    27001 tatatgattt taacttaaaa gaatggagaa gtaaaatcgc ttgggtttca caaaataatg
    27061 cagtcttatc tggcagtatt cgtgacaatc tttgtctcgg tttgaatcgc ttagtaactg
    27121 atgatgaatt gatgaaagtg ctagacttag tatcactagg tgatgagatt cgctccatga
    27181 aagagggact agatactgaa gttggtgaac gcggacgact cttgtcaggg gggcgaagcc
    27241 aaagacttca aatagctaga gcctacttaa aagatgctga aattcttata tttgatgaag
    27301 ccactgctaa tcttgatgcg gattctgagt atgcgattat cagtagcctc tattctgtac
    27361 taaaggagaa gacggttgtg attatagcgc atcgtttgtc aacggtaaaa gatgtggatt
    27421 gtattttctt cttagaggag gggaaaatca ctggctcagg aactcataag gaactactgg
    27481 aaaatcatga gcgttatgct cgttttgtgc aggagcaaat gatagagtga aggtgtcttt
    27541 tgagattcac cattttatag tctattaaag ggagcaggaa aaactccctt tttatatagt
    27601 ttgaaactat aactagctct tgaaaagaag aaaatgagtt gatgaaaata agtggtacaa
    27661 tagttactat agatttggag gaattgtatg agcaaggaat tacacattaa cacaatttta
    27721 gcacaagcag ggatcaagtc tgatgaggca acaggtgctt tggtgacacc acttcatttt
    27781 tcaacgacct atcagcatcc agagtttggt cgatctactg ggtttgacta tacgcgcact
    27841 aaaaatccaa ctcgtagtaa ggctgaggaa gtcttggcgg ctattgagtc agcagactat
    27901 gccctagcga ctagctcagg gatgtcagct attgtactgg cctttagcgt ctttccagta
    27961 ggaagtaagg tcttggcagt gcgtgatctt tacggtggtt cttttcgctg gtttaaccaa
    28021 gtggagcagg aaggtcattt ccattttacc tatgccaata cagaagaaga gttgattgcc
    28081 gagttagaaa aggatgtgga tgttctctat atcgaaaccc caaccaatcc cttgatgttg
    28141 gaatttgata tcgaaaaact agcaaaattg gctcatgcta agggtgccaa agtggtggtg
    28201 gacaatacct tctatagccc tatctaccaa cgtccgattg aagatggagc agatatcgtt
    28261 ctccattcag caaccaagta tctagcaggc cacaatgatg tcttggctgg agtggttgtg
    28321 accaatagtt tagaactata cgagaagctt ttttacaatc ttaatacaac aggggcagtc
    28381 ttgtctccat ttgacagcta ccagttgctt cgtggtctca agaccttgtc tcttcgtatg
    28441 gagcgctcaa cagctaacgc ccaagaagtg gttgcctttt tgaaggattc tccagcagtt
    28501 aaggaagttc tctacactgg tcgtggaggc atgatttcct ttaaagtagc cgatgaaaca
    28561 cgcattcctc atattttgaa cagtctcaag gtcttctctt ttgcggaaag tttgggtgga
    28621 gtggaaagtc ttattactta tccaacgact caaactcatg ctgatattcc agcagaagta
    28681 cgccattctt atggtttgac agatgacctc ttgcgtttgt ctattgggat tgaggatgct
    28741 agagatttga ttgcagattt gcgccaagcc ttagaaggat aagacaaaga tgggaaaata
    28801 tgattttaca agcctgccca accgtttagg gcaccatacc tataaatgga aagaaacaga
    28861 aacggatagt gaagttctac cagcttggat agcggatatg gactttgtag tcttgcctga
    28921 aatccaccaa gccgtgcaaa cttacgcaga ccaactggtt tatggttata cctatgccag
    28981 tgaagactta attaaggaag ttcaaaagtg ggaagctaca caatacggtt acaactttga
    29041 caaagaggct cttgtcttta tcgagggtgt ggtaccagcc atctcaacag ctattcaaac
    29101 ctttacaaaa gaaggcgagg cggttttaat taacacgcct gtctacccac cctttgctcg
    29161 cagtgtcaag ttgaataatc gtagattgat tactaattcc ttagtggaaa aggatagtct
    29221 gtttgagatt gactttgacc aacttgaaaa ggatttggtg gaagaggagg ttaaactcta
    29281 tgttctttgc aaccctcaca atcctggtgg acgtgtttgg gaaaaagaag tgttggagaa
    29341 gattggccaa ctctgccaaa aacacggtgt tttgttagtt tcggatgaga ttcaccaaga
    29401 tttgaccctc tttggtcaca aacaccagtc tttcaatacc atcaatcctg ccttcaaaaa
    29461 ttttgctatc gtcttgagca gtgccactaa aacatttaat attgctggaa caaaaaattc
    29521 ctatgcagtc attgaaaatc ctaagttgag actggctttc cagaaacgcc tgttggccaa
    29581 taatcagcat gaaatttcag gcttgggtta tttggcgaca gaagctgcct atcgatacgg
    29641 taaagattgg ctagaggaac tcaagcaagt ctttgaagac cacatcaatt atgtggtgga
    29701 tctatttgga aaagagacta aaatcaaggt catgaaaccg caaggtacct acttgatttg
    29761 gcttgacttt tcagcttatg acctgactga tgaaacattg caagagttgt tgagaaatga
    29821 agccaaggtt atcctcaacc gtggtttgga ttttggagag gaaggaagtc tccatgcccg
    29881 catcaatata gctatgccca aatctctgtt gcaagaagtc tgtcagcgga ttgtggctac
    29941 ttttgccaaa tgttaaaaat ccagccttct aggagaaaag tcttcctaga aggctatttt
    30001 cataggcgaa aatatggtat aataaaaaga taaggtaaag gtgaaaatat ggctaaattg
    30061 attccgggga aaattcgtat cgaaggtgtt gccctttatg aaactggtaa ggttgatatc
    30121 atcaaggaaa agaacaatcg gctctacgct cgcgttgcag aagaagaact gcgctatagt
    30181 ttagaggatg atttgatttt ttgtgcctgt gatttttttc aaaagagggg ctactgtgtg
    30241 catttggcag cgctagagca ttttctgaaa aatgatgagc gtggtcagga aatcttgtgg
    30301 agtctggaag aaggtcatga agaaaaagag gccgttgaaa ccaaggtgac cttgggtggc
    30361 aagtttttgg atcgaatttt atctccaaaa tcagaatgcg cctatgagtt atcagctgtg
    30421 ggtcaggtgg aagcaggaac caatcatatc ttgtggactc tgagaatcgg ccaaatcaat
    30481 agccaaaaat actatgtcat tcgggatatt ccactttttt taaggattgt cgagcagaga
    30541 aagtcgtata tgattggcaa gatttatgag gagtctcttt cttgggaagc ctttgatgat
    30601 gccagccaag aacttttgac tttcttgcac ggactaacgg aggaagggct ggttccagac
    30661 ctctttttcc aaaaccaagg tcggcacctc tttttccctc tgaccttttt tgagcagggt
    30721 gtgaatttac tgatggcctt gccccatttt caatttgacc atcaagtgga tagctaccaa
    30781 accctgcttt ttcaggatat gcatgctgat gccaatctat ttgcctttac agtaacagaa
    30841 tattcggact attttgaaat ggaaatcagt gagagtccta gggtcaatgt cttttatcaa
    30901 ggagcagtac ttttccataa aggtcaggtt tattttttaa cagaccagca gatgcgtctt
    30961 ctcaaggaaa ttaaagcgtt acctgtgggt caacaaggaa agaaatacct gcaatttgat
    31021 agcagtgatc gagacaaatt ggcctcctgt ctgactcttt ttatccagat gggccccgtt
    31081 tcagctccag aacgtctaca aatcaaaact tttgcgccct ctttttactt tgacagggaa
    31141 gaagataatc ggattcgttt agaaattcag tttgattatg gaaatagaca ggtatctagc
    31201 cgacaagagc tagaagaatt accattttca agtgacgcgg acttagaaga aagaattttc
    31261 caagtctgtt tggctgcggg ctttgaagcc gattttcaat cttggcgtca ggccttaaaa
    31321 gcggagtctg tctatcattt ttttcatgat atcattccag tctttgaaaa actcgggcat
    31381 gttgacctat cagataaact agaagagctt tatagcctag cgagtcctca ggtgcaaatt
    31441 gcctccaagg gaggtctctt ggaaatccag tttgattttc aagatattgc ccaggaggaa
    31501 atcgaccaag ccatgcaggc cttggttgcc aatcaggatt tttatattga ttcgtctaat
    31561 caagtttact ttttcgatga agaaaccaag aaaattcgcc aaaatctaca ggaactgggg
    31621 caatttgaat taaaagatgg gaccttgcag gctcggaaat ccttggccta cagtttagct
    31681 catctctttg aagggcgaga ccgtgtttct ttttcacaag aattccagaa tctggcccag
    31741 gatttgacgc atccagagga ctttcctttg caagcaactc aggtcaaggc tgacttgcga
    31801 gattatcagg aaaagggaat tggctggttg cagatgctcc atcattatgg ttttggtggg
    31861 attttggctg atgatatggg acttgggaaa acccttcaga ccattgcttt tttgactagt
    31921 caagtgacaa aagaaagtcg ggttttgatt ctcgctccgt cgggtttgat ttacaactgg
    31981 gcagatgagt ttcagaaatt tgctccacag ttggatgtga ctgttgttca tggtttgaaa
    32041 gcaagtcgtg aggagattct tgccgagagc catcaaatct atgtgactag ctatgccacc
    32101 ttccgtcagg acagtgagtt ttatcaaggg atggcctttg acttcctttt cttagatgag
    32161 gctcaggtca tgaaaaatgc ccagaccaag attgcccaga ccttgagaca atttgtggtg
    32221 ccgtcggtct ttgccttgtc aggaactccg attgaaaacc atctgggtga gttgtggtct
    32281 attttccaaa tcgtcatgcc cggacttttg ccaagcaaga aagaatttat gaaattgcca
    32341 gcagagcgag tggctcagtt tatcaagcct ttcgtgatgc gacgcaagaa agaagaagtt
    32401 ctgactgaat tgccagactt gattgaggtg gtttataaaa atgaactgga agaccagcaa
    32461 aaggctattt accttgccca gttacaacag atgcgagacc atctggctca agtgtcagaa
    32521 caggaatttc agcgaagtcg tgtggaaatt ttatctggtt tgatgcgctt gcgtcaaata
    32581 tgtgacactc ctgccctgtt tatggaagat tatcagggag ccagcggcaa actagatagt
    32641 ctccgagacc tattggtaca ggtggcagac ggcggacacc gtgtcttgat tttctctcag
    32701 ttcaagggaa tgttggaaaa aattgaacaa gaactgccag acttgggcct gacttccttt
    32761 aaaattacgg gttcaacccc agccaaggaa agacaagaca tgaccaaggc ctttaaccaa
    32821 ggagaaagag atgcctttct gatttccctc aaggctggtg gggtcggtct gaacctgaca
    32881 ggtgcagata cagtgatctt ggttgacctt tggtggaatc ctgcggtgga agcgcaagcc
    32941 attggccgtg cccatcggat gggtcaggaa gaaacggttg aggtctatcg cttggtgacc
    33001 aagggaacca ttgaagaaaa aattcaggaa ctccaagaac aaaagaaaca tctggtgtca
    33061 caagtattgg acggcacaga gtcacgtggt agtctgaccc tagcagaaat tagagaaatt
    33121 ttgggaattt ctgaagccag cacttgaaaa atcaagacaa tacgctataa tgaagtgttg
    33181 aaaggaaata gaaaatgaaa caagaacgat ttccattggt gtcagatgac gaggtcatgt
    33241 tgactgaaat gccagtcatg aatctctatg atgagtctga tctgatcagt aatatcaagg
    33301 gtgagtatcg agataaaaat tatttagaat gggctcctat tgctgaagaa aaaccagtaa
    33361 aaccgattga aaagcaagtc gaaaaaccta aaaaggctcc tttaggggtt aaaaaagaag
    33421 gaaagagcta tgcggaggtg gcgcgtgaag aagcgcgtgc ggacttgaaa aagaaacgct
    33481 ctgctaacta cctaactcag gatttcagcc ttgcgagacg tcattctcag cccagtctag
    33541 ttagacaggg caatcaaccg acagctcctt tccaaaagga aaatcctggt gaatttgtca
    33601 aatatagcca aaaattgacc cagtctcatt atatcttggc ggaagaagtt cattctatcc
    33661 ctaccaagaa tgaagaagtg tcagcacctg ctccaaagaa aaacaattat gattttctaa
    33721 agaagagcca aatctacaat aaaaaaagta aacaaacaga acaagaacgt cgggttgccc
    33781 aagagttgaa tctgaccaga atgacagaat aggggagaaa acatgtcaaa gacatatcat
    33841 tttatcggaa ttaagggatc agggatgagt gccttggcct tgatgttgca ccagatgggg
    33901 cacaaggttc agggatcaga tgttgaaaag tactacttta cccaacgcgg tcttgagcag
    33961 gcaggaatta ccattcttcc ttttgatgaa aaaaatctag acggtgatat ggaaattatc
    34021 gctggaaatg cctttcgtcc agataacaac gtcgaaattg cctatgcgga ccaaaatggt
    34081 atcagctaca aacgttacca tgagtttcta ggtagcttta tgcgtgactt tgttagcatg
    34141 ggagtagcag gagcacatgg aaaaacttca acgacaggta tgttgtctca tgtcttgtct
    34201 cacattacag ataccagctt cttgattgga gatgggacag gtcgtggttc ggccaatgcc
    34261 aaatattttg tctttgaatc tgacgaatat gagcgtcact tcatgcctta ccacccagaa
    34321 tactctatta tcaccaacat tgactttgac catccagatt atttcacaag tctcgaggat
    34381 gtttttaatg cctttaacga ctatgccaaa caaatcacca agggtctttt tgtctatggt
    34441 gaagatgctg aattgcgtaa gattacgtct gatgcaccaa tttattatta tggttttgaa
    34501 gctgaaggca atgactttgt agctagtgat cttcttcgtt caataactgg ttcaaccttc
    34561 accgttcatt tccgtggaca aaacttgggg caattccaca ttccaacctt tggtcgtcac
    34621 aatatcatga atgcgacagc cgttattggt cttctttaca cagcaggatt tgatttgaac
    34681 ttggtgcgtg agcacttgaa aacatttgcc ggtgttaaac gtcgtttcac tgagaaaatt
    34741 gtcaatgata cagtgattat cgatgacttt gcccaccatc caacagaaat tattgcgacc
    34801 ttggatgcgg ctcgtcagaa atacccaagc aaggaaattg tagcagtctt tcaaccgcat
    34861 acctttacaa gaaccattgc cttgttggac gactttgccc atgctttaaa ccaagcagat
    34921 gctgtttatc tagcgcaaat ttatggctcg gctcgtgaag tagatcatgg tgacgttaag
    34981 gtagaagacc tagccaacaa aatcaacaaa aaacaccaag tgattactgt tgaaaatgtt
    35041 tctccactcc tagaccatga caatgctgtt tacgtcttta tgggagcagg agacatccaa
    35101 acctatgaat actcatttga gcgtctcttg tctaacttga caagcaatgt tcaataggat
    35161 gttcccatgg aaattccaat taagatcatt caggcaagca agtctgattt gcctgagata
    35221 ggggcacttc agacctcgtc ttttccagct gaaaagcagc aactttccca tattttagaa
    35281 gaaagtatcc gtaagtgtgc ggataccttt cttctagcta gggatgaaaa tcaactttta
    35341 ggctatattt tatcaagtcc ccagtcagac aatccgcaat gtctaaaagt acattcttta
    35401 gtcatcgagt ctgaccatca gagacagggc ctgggaacac ttcttcttgc agccttgaaa
    35461 gaggtggcag ttgagctgga ttacaaaggg attcgtttgg agagtcctga tgagctgctt
    35521 tcctattttg aaatgaacgg ttttgttgat gaagaagcaa ctttgctcta tgcaactagc
    35581 cagggctata gtatgatttg gtttaatccc ttttatctgg aggaacaatg aaaatcagac
    35641 aagcaagatt agaagatttg gatcggattg ttgaacttga atttgaaaat ttttcggtcg
    35701 aagaagccat tcctccttct gtctttgaag cacatttgag agaaattcag acctcttttc
    35761 tggttgctga aaaagaagga agaatcatgg gttatatcga aggaccagtt ggcctgcacc
    35821 gccatctgca agaccagtct tttacagaag aaataaaaga ctatagtcat gagcctggtg
    35881 gttatatctc tgtgacctgt ctgtctattg ccaaggaggc acagggactc ggactgggtc
    35941 agaaattgct gacagccttg aaagaagttg ctcttgaaga tgagagagat ggcattaatc
    36001 taacctgtca tgactatctc atcgcctact atgaaaaaca tggatttgtc aacgaaggcc
    36061 agtcccagtc aacctttgca ggggaaacat ggtatgatat ggtctgggaa atgaaaaaat
    36121 aagttaggaa aagtatcata gattgctttt tttggacttt taagatataa tattatggat
    36181 tatcaacaag gaggaaaaac ttttgagtga aaagtcaaga gaagaagaga aattaagctt
    36241 taaagagcag attctgagag atttagaaaa agtaaaaggc tatgatgaag ttctgaaaga
    36301 agatgaggca gtagttcgca ctcctgcaaa tgaaccttca actgaagaac tcatggctga
    36361 ttccttgtca acggtagagg agattatgag aaaagctcct accgtgccta ctcacccaag
    36421 tcaaggtgta ccagcttctc cagcagatga gattcaaaga gaaactcctg gtgttccaag
    36481 tcatccaagt caagatgtac cttcttctcc agcggaagaa agtggatcaa gaccaggtcc
    36541 aggtcctgtt agacctaaga aacttgaaag agaatacaat gaaaccccaa caagggtagc
    36601 tgtttcctat acgacggcag agaaaaaagc agaacaagca ggtccagaaa cacctacgcc
    36661 tgctacagaa acagtggata tcatcagaga tacatcacgt cgtagccgta gagaaggagc
    36721 aaaacccgtt aagcctaaga aagagaagaa gtcacatgtg aaagcttttg tgatttcatt
    36781 ccttgtattc cttgccttgc tctcagcagg tggttacttt ggttaccagt acgtgctaga
    36841 ttccttatta cctatcgatg ctaattctaa gaaatatgtg acggttggaa ttccagaagg
    36901 ttcaaacgtt caagaaatcg gtacgacgct tgaaaaagct ggtttggtaa agcatggtct
    36961 gatttttagt ttttatgcca agtataaaaa ttataccgac ttgaaagcag gttactacaa
    37021 tttgcaaaag agtatgagta cagaagactt actcaaagag ttgcaaaaag gtggaacaga
    37081 tgaaccgcaa gaacctgtac ttgcgacttt gacaattcca gaaggttata ccttggatca
    37141 gattgctcaa actgtgggtc aattgcaagg tgacttcaaa gagtctttga cagcggaggc
    37201 tttcttggct aaagttcaag atgagacgtt tatcagtcaa gcagtagcga aatatcctac
    37261 tttactggaa agtttgcctg taaaagacag cggtgcgcgt tatcgtttgg aaggatacct
    37321 tttcccagct acatactcta tcaaggaaag cacaactatt gagagcttga ttgatgagat
    37381 gttagctgct atggataaga acctatctcc ttactatagt actatcaaat ctaaaaactt
    37441 gactgtcaat gagttgttga ccattgcttc cttggtcgaa aaagaaggtg ccaagacaga
    37501 agatcgtaag ctcattgcag gtgtatttta caatcgtttg aatcgtgata tgccacttca
    37561 aagtaatatt gcaatcttgt atgcccaagg aaaactgggg caaaatatca gtctagctga
    37621 ggatgttgcg attgatacca acattgattc accttataat gtttataaaa atgtaggtct
    37681 catgcctggt ccagtcgata gtccaagtct ggatgcgatt gagtcaagca tcaatcaaac
    37741 taagagcgat aacctctact ttgtagcaga tgtcacagaa ggcaaggtct actatgctaa
    37801 caatcaagaa gaccacgacc gcaatgtcgc tgaacatgtc aacagcaaat taaactaaac
    37861 aaactaaaat tatgtgatac ttcacataat tttctttaga aaatattatc agaagaaagt
    37921 tgagaaaaat ggcagaaaaa acatatccta tgacccttga ggaaaaggag aaacttgaaa
    37981 aagaattaga agaattgaaa ttggttcgtc gaccagaagt ggtagaacgc attaagattg
    38041 cccgttcata cggtgatctt tcagaaaaca gtgagtacga agcagctaag gatgaacaag
    38101 cctttgtcga aggacaaatc tctagcttag aaacaaaaat ccgctatgct gaaatcgtca
    38161 atagcgacgc agttgcccag gacgaagtag cgattggtaa aacagtcacc atccaagaaa
    38221 ttggtgagga cgaagaagaa gtttatatta tcgtaggttc agctggtgcg gatgcctttg
    38281 caggtaaggt ttcaaatgaa agcccaattg ggcaggcctt gattggcaag aaaacaggtg
    38341 acacagcaac cattgaaacg cctgttggta gctatgatgt aaaaatcttg aaggttgaaa
    38401 aaacagccta aaaacagaaa aaggagtggg gaggcgatgt gcttcactca ctcctttttc
    38461 cattttgcta ttcttcgaaa atctcttcaa accacgtcag cgtcgccttg ccgtatgtat
    38521 ggttactgac ttcgtcagtt tcatctacaa cctcaaaaca gtgttttgag ctaacttcgt
    38581 cagtttcatc tacaacctca aaaccatgtt ttgagccgac ttcgtcagtt tcatctacaa
    38641 cctcaaaacc atgctttgag ctgacttcgt cagtttcatc tacaacctca aaaccatgct
    38701 ttgagctgac ttcgtcagtt ctatctacaa cctcaaaaca gtgttttgag caacctgcgg
    38761 ctagtttcct agtttgctct ttgattttta ttgagtatta gatgtatagt gcattgaagt
    38821 taaaaatagt acactttgat ttctaagaca ttgttagaaa ttagattaaa ttccttaatc
    38881 gatttgcgca tgttttattt catttcacta tactttagat tggaaaataa ggaatcacat
    38941 aaatcgcaag aaatagggaa tctgattcgt gtttataacc gttcaaaaag agaagaggct
    39001 gaaagtgagc cacttaatct ttatgtcgaa gatgaaaagg gcaatctcct ggcaggtttg
    39061 atagcagaga cttttggaaa ttggctagaa atcgagtatt tgtttgtaaa agaggaactg
    39121 cgagggcaag gaatcggttc aaaactattg caacaggcag aaagtgaagc taagaatcga
    39181 aattgttgtt ttgcctttgt caatacttac cagttccagg caccggactt ttatcagaaa
    39241 catggctaca aggaagtttt ttctttgcaa gattatctct acattaggca aagatattat
    39301 taccaaaaga atctgtaagg tgagtatgga agtggactga tatatatgag taactatcga
    39361 taaataccag ttataaccta gaaattgtgt ctttctagac gatattgagg acaatataat
    39421 cgtagcccca aaattaggaa ttaaggctta tcaggttaag aaaagaagtg atgttgttga
    39481 tattttaaaa caatttattt aaactaaaaa ctctctatct ttttaaattg acattaatcc
    39541 gagattcccc tctcggattt tgtgttgctt tcattaaatt tacaggtaag acttgctagt
    39601 ttcagtaaat atacacaagt ttttcagaat agagtgaaat ccaaaagaaa atggaaataa
    39661 tgaaagctct tgtaggcata agaaaacacc tctgtattat acttgttgtt caaccacaaa
    39721 cacaagaaag gcacagagat gcaagacaat tatactacaa aagccaaaca tttgacaatc
    39781 gatagccgtc gcttaatcga aagatggaaa aaagaaggga aatcaaatag agaaattgcc
    39841 tctctacttg gaaaagctcc tcaaactatc cacactgaaa tcaagcgcgg gacagtccga
    39901 caatgtcttg gaaaagggcg cttcaaagaa gtttattctg ccgactatgc tcaacagtct
    39961 tatgaaaaca atcgcaagcg ctcggtcaag aaatcaagct tgaccaagga actaaaggaa
    40021 aagattctcc actatcataa ccaaaaattt tcgcctgaaa tgatggtgat ggctaaaggg
    40081 gttaacgtgg gaatttcaac catttacta
//
LOCUS       DBERDK010000012        39808 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.012, whole genome shotgun sequence.
ACCESSION   DBERDK010000012 DBERDK010000000
VERSION     DBERDK010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 39808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39808
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.012"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            9..89
                     /locus_tag="ACL73L_000583"
     tRNA            9..89
                     /locus_tag="ACL73L_000583"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43..45,aa:Tyr,seq:gta)
     gene            94..164
                     /locus_tag="ACL73L_000584"
     tRNA            94..164
                     /locus_tag="ACL73L_000584"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:126..128,aa:Trp,seq:cca)
     gene            179..251
                     /locus_tag="ACL73L_000585"
     tRNA            179..251
                     /locus_tag="ACL73L_000585"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:212..214,aa:His,seq:gtg)
     gene            262..333
                     /locus_tag="ACL73L_000586"
     tRNA            262..333
                     /locus_tag="ACL73L_000586"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:294..296,aa:Gln,seq:ttg)
     gene            343..426
                     /locus_tag="ACL73L_000587"
     tRNA            343..426
                     /locus_tag="ACL73L_000587"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:377..379,aa:Leu,seq:caa)
     gene            533..1810
                     /locus_tag="ACL73L_000588"
     CDS             533..1810
                     /locus_tag="ACL73L_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcysteine synthetase"
                     /protein_id="HGS0771611.1"
                     /translation="MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEV
                     MKDLFHYLVSTLDLTVAKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQE
                     VENRFNNYMNVIQRKLAESNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNI
                     IKSDLHHFPEYGTFICGSQVQLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWD
                     TKISRDIFWEESMHGIYPENVGVNARLLNDETDFFDYLNHSAIFTAERDGQTYYFYPI
                     QAGDYLATSEIQAFALNGDEVIIYPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLD
                     RTFASAAFHLGLLVNLDKLEAYLETAPFFKVFGYDYKSLRRQFSKKNLTDEEETTIIE
                     FSKDLLLLAEEGLVVRNKEEMTYLQPLREELSL"
     gene            1886..3370
                     /gene="thrC"
                     /locus_tag="ACL73L_000589"
     CDS             1886..3370
                     /gene="thrC"
                     /locus_tag="ACL73L_000589"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HGS0771612.1"
                     /translation="MTLVYQSTRDANNTVTASQAILQGLATDGGLFTPDTYPKVDLNF
                     DKLKDASYQEVAKLVLSAFLDDFTVEELDYCINNAYDSKFDTPAIAPLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFADVPGT
                     EIIVFYPKGGVSKVQELQMTTQNGDNTHVIAIDGNFDDAQTNVKHMFNDVALRERLAA
                     NKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIVAGDKVNFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKKREFKVTTSPSMDILVSSNLERLIF
                     HLLGNDAVKTAKLMNALNSQGQYELTNFDAEILDLFAAEYATEEETAAEIKRVYESDS
                     YIEDPHTAVASAVYRKYQSATGDVTKTVIASTASPYKFPVVAVETVTGKAGLTDFEAL
                     AQLHEISGVAVPPAVDGLEIAPIRHKTTVAAADMKAAVEAYLGL"
     gene            3431..4711
                     /locus_tag="ACL73L_000590"
     CDS             3431..4711
                     /locus_tag="ACL73L_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HGS0771613.1"
                     /translation="MFKKNKDILNIALPAMGENFLQMLMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIGLLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGIAGVAWGTILSRLVGLVILWSQLKLPYGKPTFGLDK
                     ELLTLALPAAGERLMMRAGDVVIIALVVSFGTEAVAGNAIGEVLTQFNYMPAFGVATA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGVPLTHLYTTDSLAVE
                     ASVLVTLFSLLGTPMTTGTVIYTAVWQGLGNARLPFYATSIGMWCIRIGTGYLLGIVL
                     GWGLPGIWAGSLLDNGFRWLFLRYRYQRYMSLKG"
     gene            4715..5335
                     /locus_tag="ACL73L_000591"
     CDS             4715..5335
                     /locus_tag="ACL73L_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HGS0771614.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIEETFAQFSIPYDKEKVREF
                     IFKYSVQDLLVRVAEDRNLDVEVLSQVRAQSLTEKNAQVVLMPGAREVLAWADESGIQ
                     QFIYTHKGNNAFTILKDLGVESYFTEILTSQSGFVRKPSPEAATYLLDKYQLNSDNTY
                     YIGDRTLDVEFAQNSGIQSINFLESTYEGNHRIQALADISRIFETK"
     gene            5501..6643
                     /locus_tag="ACL73L_000592"
     CDS             5501..6643
                     /locus_tag="ACL73L_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HGS0771615.1"
                     /translation="MKKRMLLASTVALSFAPVLATQAEEVLWTARSVEQIQNDLTKTD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPQADSSEEVTTATADLTTNQVTVDDQTVQVADLSQPIAEAPKEVASSSEVTKTV
                     IASEEVAPSTGTSVPEEQTAETTRPVEEATPQETTPAEKQETQASPQAASAVEVTTTS
                     SEAKEVASSNGATAAVSTYQPEETKTISTTYEAPAAPDYAGLAVAKSENAGLQPQTAA
                     FKEEIANLFGITSFSGYRPGDSGDHGKGLAIDFMVPESSELGDKIAEYAIQNMASRGI
                     SYIIWKQRFYAPFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG"
     gene            7071..7494
                     /locus_tag="ACL73L_000593"
                     /pseudo
     CDS             7071..7494
                     /locus_tag="ACL73L_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890080.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
     gene            7505..7864
                     /locus_tag="ACL73L_000594"
     CDS             7505..7864
                     /locus_tag="ACL73L_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HGS0771616.1"
                     /translation="MSIILTTIVALEHFYIFYLESIATQSDATSRVFNMEKEELAHPS
                     VSSLFKNQGIYKDLLGVFLLYGIYFSQNLEIVTIFVLFVIGAATYGSLTADKKIILKQ
                     GGSAILALISILLFKYT"
     gene            7971..8614
                     /gene="pcp"
                     /locus_tag="ACL73L_000595"
                     /pseudo
     CDS             7971..8614
                     /gene="pcp"
                     /locus_tag="ACL73L_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
     gene            complement(8642..9499)
                     /locus_tag="ACL73L_000596"
     CDS             complement(8642..9499)
                     /locus_tag="ACL73L_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HGS0771617.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSSFLNP
                     LSRIHGTVLEQPFFSILGQILQAYLFPLLVSFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIYLLFYSSFLAKKFVTLHPEFP
                     NLDLSSVETERFLMVFGLYFLASLILIIVGNILYIPQYYAYSQVEFLLCYSLDLGQVP
                     PRRILKTSRSFMKGYKFQHFVLDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYRA
                     VLARKRPKA"
     gene            9629..10771
                     /gene="tgt"
                     /locus_tag="ACL73L_000597"
     CDS             9629..10771
                     /gene="tgt"
                     /locus_tag="ACL73L_000597"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HGS0771618.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREYFVEKYGYNKSGRNF"
     gene            10869..12686
                     /locus_tag="ACL73L_000598"
     CDS             10869..12686
                     /locus_tag="ACL73L_000598"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HGS0771619.1"
                     /translation="MRIKWFSLIRIIGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNNEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     AGVLGFMTNFYELLTGGSYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQAKSN
                     GQLKGMVFLLSAAAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSMLATIVGV
                     RQTTSLVKQLDKIWDLRKTLVVFGGGFGFLVLLTFFVKFTYLFAYLIGFLLASLAALA
                     MILAARVLHEKTHHIQEPKIISFLADTSYAVYLFHWPSYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIAGKNTPILQTLRPLPYIHAILAAGTGILTFIVCMVTLLAPQV
                     GAFETDLTVNGLKQAATNIGQTKVMAERADANSLGIADGTMLIGDSVALRANTALQTA
                     LPGAQINAQVSVTTKTANEIMLNNSQNKFLPKTVVIATGVNNPENYKDDWDSIVKNLP
                     KGHRMILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITIADWNQVAKEHPEIWAG
                     TDQVHFGSESSTIEAGAKLYADTIATALQTAQDKPVKSK"
     gene            12839..13990
                     /gene="nagA"
                     /locus_tag="ACL73L_000599"
     CDS             12839..13990
                     /gene="nagA"
                     /locus_tag="ACL73L_000599"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HGS0771620.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAA
                     NGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAVDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPHTYAELICDGHHVDPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
     gene            14153..15211
                     /locus_tag="ACL73L_000600"
     CDS             14153..15211
                     /locus_tag="ACL73L_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HGS0771621.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSGTILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLIDGMQAEYLRVPHADNTLYHTPEDLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLETALSFGATHKVN
                     SSDPKKAIKEIYDLTDGRGVDVAIEAVGIPETFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            15362..15727
                     /locus_tag="ACL73L_000601"
     CDS             15362..15727
                     /locus_tag="ACL73L_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HGS0771622.1"
                     /translation="MYRVIEMYGDFEPWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            15803..16744
                     /gene="comGA"
                     /locus_tag="ACL73L_000602"
     CDS             15803..16744
                     /gene="comGA"
                     /locus_tag="ACL73L_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HGS0771623.1"
                     /translation="MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYAYDQKIASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVV
                     RASLTGATVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAN
                     RDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            16692..17708
                     /gene="comGB"
                     /locus_tag="ACL73L_000603"
     CDS             16692..17708
                     /gene="comGB"
                     /locus_tag="ACL73L_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HGS0771624.1"
                     /translation="MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSSLLDKQCVTQMRTGLSQGKSFSEMMESLGCSSTIVTQLSLAEVHGNLHLSLGKIEE
                     YLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
                     LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQ
                     GMELTQIFQMMQEQGSQLFKEIGQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIVLLYAAMLMPMYQNM
                     EVNF"
     gene            17710..18036
                     /gene="comGC"
                     /locus_tag="ACL73L_000604"
     CDS             17710..18036
                     /gene="comGC"
                     /locus_tag="ACL73L_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="comG operon protein ComGC"
                     /protein_id="HGS0771625.1"
                     /translation="MKKMMTFLKKAKVKAFTLVEMLVVLLIISVLFLLFVPNLTKQKE
                     AVNDKGKAAVVKVVESQAELYSLEKNEDASLRKLQADGRITEEQVKAYNEYYTKNGGA
                     NRKVND"
     gene            18029..18433
                     /gene="comGD"
                     /locus_tag="ACL73L_000605"
     CDS             18029..18433
                     /gene="comGD"
                     /locus_tag="ACL73L_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HGS0771626.1"
                     /translation="MIKAFTMLESLLVLGLVSILALGLSGSVQSTFAAVEEQIFFMEF
                     EELYRETQKRSVASQQKTSLNLDGQTLSNGSQKLTVPKGIQAPSGQSITFDRAGGNSS
                     LAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            18396..18698
                     /gene="comGE"
                     /locus_tag="ACL73L_000606"
     CDS             18396..18698
                     /gene="comGE"
                     /locus_tag="ACL73L_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGE"
                     /protein_id="HGS0771627.1"
                     /translation="MEKLNALRKQKIRAVILLEAVVALAIFASIATLLLGQIQKNRQE
                     EAKILQKEEVLRVAKMALQTGQNQVNINGVEIQVFSSEKGLEVYHGSEQLLAIKEL"
     gene            18661..19122
                     /gene="comGF"
                     /locus_tag="ACL73L_000607"
     CDS             18661..19122
                     /gene="comGF"
                     /locus_tag="ACL73L_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HGS0771628.1"
                     /translation="MVQNSCWQSKSYKVKAFTLLESLLALLVISGGLLLFQAMSQLLI
                     SEVRYQQQSEQKEWLLFVDQLEVELDRSQFEKVEGNRLYMKQDGKDIAIGKSKSDDFR
                     KTDASGRGYQPMVYGLKSAQITEDNQLVRFHFQFQKGLEREFIYRVEKEES"
     gene            19100..19513
                     /gene="comGG"
                     /locus_tag="ACL73L_000608"
     CDS             19100..19513
                     /gene="comGG"
                     /locus_tag="ACL73L_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HGS0771629.1"
                     /translation="MWKKKKVKAGVLLYAVTMAAIFSLLLQFYLNRQIAHYQDYALNK
                     EKLVAFAMAKRTKDKAEQESGEQVFNLGQVSYQNKKTSLVTTVRTSKSQYEFLFPSVK
                     IKEEKRDKKEEVATDSSEKVEKKKSEEKPEKKENS"
     gene            19650..20237
                     /locus_tag="ACL73L_000609"
                     /pseudo
     CDS             19650..20237
                     /locus_tag="ACL73L_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679792.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            20298..21251
                     /locus_tag="ACL73L_000610"
     CDS             20298..21251
                     /locus_tag="ACL73L_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGS0771630.1"
                     /translation="MDFEKIEQAYIYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELNQVKDNNQALKRLALRKEEWLKTYQFLLMKAGQTEPLQANHQFTPDAIALLLVFIV
                     EELFKEEEITILEMGSGMGILGATFLTSLDKKVDYLGMEVDDLLIDLAASMADVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGL
                     KYLKSDGYAIFLAPSDLLTSPQSDLLKEWLKEEASLVAMISLPENLFANAKQSKTIFI
                     LQKKSEIAVEPFVYPLASLQDASVLMKFKENFQKWTQGTEI"
     gene            21302..22492
                     /locus_tag="ACL73L_000611"
     CDS             21302..22492
                     /locus_tag="ACL73L_000611"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HGS0771631.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGRSEQQILDIENHIQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
                     PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKYIGQYLAVLNGADAIVFTAGVGE
                     NAENFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVE
                     RLKK"
     gene            22493..22624
                     /locus_tag="ACL73L_000612"
     CDS             22493..22624
                     /locus_tag="ACL73L_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771632.1"
                     /translation="MKLKKYSIQGVGKVIFPASFSDEIVQNLAMIGFFEKYGIIVVI"
     gene            22641..23012
                     /gene="rnpA"
                     /locus_tag="ACL73L_000613"
     CDS             22641..23012
                     /gene="rnpA"
                     /locus_tag="ACL73L_000613"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HGS0771633.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNHFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGSLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYREGNGSEKETKVD"
     gene            22987..23811
                     /locus_tag="ACL73L_000614"
     CDS             22987..23811
                     /locus_tag="ACL73L_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HGS0771634.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNGVTSDITAESADFWSKLVYF
                     FAEIIRFLSFDISIGVGIILFTVLIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYP
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGHFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGATTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQAQKDLENRKRKAKKKAQKTK"
     gene            23830..24816
                     /gene="jag"
                     /locus_tag="ACL73L_000615"
     CDS             23830..24816
                     /gene="jag"
                     /locus_tag="ACL73L_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HGS0771635.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIEAISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVDAIKKI
                     EEEGQGISDEVKAEILKHERHASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QAESQELEDLGLKVETNFDIEQVATEVMAYVQTIIDDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNCYSRTFYITINVNDYVEHRAEVLQTYAQKL
                     AIRVLEEGRSHQTDPMSNSERKIIHRIISRMDGVTSYSEGDEPNRYVVVDTE"
     gene            24867..25490
                     /locus_tag="ACL73L_000616"
     CDS             24867..25490
                     /locus_tag="ACL73L_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI_0552 family protein"
                     /protein_id="HGS0771636.1"
                     /translation="MLNKIRDYLDFAGLQYRNPDKAGAEREKMLAFRHKGQEARKAFT
                     ELAKAFQASHPEWQLQQTSQWMNQAQRLRPHFWVYLQRDGQVTEPMMALRLYGTSTDF
                     GISLEVSFIERKKDEQTLGKQAKVLDIPTVKGIYYLTYSNGQSQRWEANEEKRRTLRE
                     KVRSQEARKVLVKVDVPMTENSSEEEIVEGLLKSYSKILPYYLATRK"
     gene            25621..27078
                     /locus_tag="ACL73L_000617"
     CDS             25621..27078
                     /locus_tag="ACL73L_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HGS0771637.1"
                     /translation="MEVVSSVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKATVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIAAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAATVSLMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLSIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFTFGLIGQLITIVLLIVFKNPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLASYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFLLVIPQLQFAKAKDKEKYYNGEVQEEA"
     gene            27101..27382
                     /locus_tag="ACL73L_000618"
     CDS             27101..27382
                     /locus_tag="ACL73L_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGS0771638.1"
                     /translation="MVKVLAACGNGMGSSMVIKMKVENALRKLNQTDFTVNSCSVGEA
                     KGLAVGYDIVIASLHLIQELEGRTNGKLIGLDNLMDDKEITEKLSQALQ"
     gene            27459..27944
                     /locus_tag="ACL73L_000619"
     CDS             27459..27944
                     /locus_tag="ACL73L_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002267837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGS0771639.1"
                     /translation="MNLKQALIDNDSIRLGLEANNWKEAVKVAVDPLIESGAILPEYY
                     DAIIESTEEYGPYYILMPGMAMPHARPEAGVQSDAFSLITLQNPVVFSDGKEVSVLLA
                     LAATSSKIHTSVAIPQIIALFELDDSIARLQACQTKEDVLAMIEESKDSPYLEGLDLE
                     S"
     gene            27961..28626
                     /locus_tag="ACL73L_000620"
     CDS             27961..28626
                     /locus_tag="ACL73L_000620"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HGS0771640.1"
                     /translation="MTKRIPNLQVALDHSDLQGAIKAAVSVGQEVDIIEAGTVCLLQV
                     GSELAEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAADPAGAARAFKDEIKR
                     IWG"
     gene            28630..29493
                     /locus_tag="ACL73L_000621"
     CDS             28630..29493
                     /locus_tag="ACL73L_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HGS0771641.1"
                     /translation="MVRPIGIYEKATPTHFTWLERLNFAKELGFDFVEMSIDERDERL
                     ARLDWSKEERLEVVKAIYETGVRIPSICFSGHRRYPLGSKDPVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPQTRQRFIKNLRKACDWAEEAQVVLAIEIMDDPFIN
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIAALHLKDTYAVTES
                     SRGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAIQEAQAF
                     LYPLIKKAGLM"
     gene            29495..30199
                     /locus_tag="ACL73L_000622"
     CDS             29495..30199
                     /locus_tag="ACL73L_000622"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HGS0771642.1"
                     /translation="MNQVINAMRKRVCAANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDLPTHVQLYKAWSEIGSVVHTHSTE
                     AVAWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVAYEKDTGLVIVEEFEHRGL
                     NPVEVPGIVVRNHGPFTWGKNPENAVYHSVVLEEVSKMNRFTEQINPRVEPAPQYILE
                     KHYQRKHGPNAYYGQK"
     gene            30376..32049
                     /locus_tag="ACL73L_000623"
     CDS             30376..32049
                     /locus_tag="ACL73L_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HGS0771643.1"
                     /translation="MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKI
                     NAALVDEALHIISIPRIGIHLTEEQRDACCKLLSEVDSYDYIMSAHERMMIMLLWIGI
                     SKERITIEKLIELTEVSRNTVLNDLNSIRYQLTLEQYQVTLQVSKSQGYHLHAHPLNK
                     IQYLQSLLYHIFMEENATFVSILEDKMKERLDDECLLSVEMNQFFKEQVPLVEQDLGK
                     KINHHEITFMLQVLPYLLLSCHNVEQYQERHQDIEKEFSLIRKRIEYQVSKKLGERLF
                     QNFEISLSGLEVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIE
                     NKDDLLRNLMIHCKALLFRKTYGIFSKNPLTKQIRSKYGELFLVTRKSAEILEGAWFI
                     RLTDDDIAYLTIHIGGFLKYTPSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFPNEQI
                     DTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIF
                     RNKSKSFSENLSSLISSYIVDSKLASKFQEEVQTLINQEIVVQAFLEDI"
     gene            32161..33252
                     /gene="ulaG"
                     /locus_tag="ACL73L_000624"
     CDS             32161..33252
                     /gene="ulaG"
                     /locus_tag="ACL73L_000624"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HGS0771644.1"
                     /translation="MPNVKEITRESWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNPKLEHVKFIGPYHCGRIWEGWGVPKE
                     RIIVVKPGDTIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLAVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDFKIDVALNNYGENPVGIQDKMTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKMRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            33366..35342
                     /gene="tkt"
                     /locus_tag="ACL73L_000625"
     CDS             33366..35342
                     /gene="tkt"
                     /locus_tag="ACL73L_000625"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HGS0771645.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLH
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMDEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFAQAERFLAAEYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVADYKEAHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDTNRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVSAAKEL
                     ASQGEKIRVISMPSTDVFDKQNAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAV
                     LGIDTFGASAPAPKVLAEYGFTVENLVKIVRNLK"
     gene            35459..35758
                     /gene="yajC"
                     /locus_tag="ACL73L_000626"
     CDS             35459..35758
                     /gene="yajC"
                     /locus_tag="ACL73L_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HGS0771646.1"
                     /translation="MNPNITFLIMLVGMMALMFFMQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVDGVYLTFELAAIKTVLPLKETASLEGAIEK"
     gene            35805..36233
                     /locus_tag="ACL73L_000627"
     CDS             35805..36233
                     /locus_tag="ACL73L_000627"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HGS0771647.1"
                     /translation="MKKLVFVCLGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQEYEIPYDKNKTSLQISKEDFEAFDYIIGMDASNISDLRQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYRRVQEGCQAWLERLEKES"
     gene            36235..36645
                     /locus_tag="ACL73L_000628"
     CDS             36235..36645
                     /locus_tag="ACL73L_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MORN repeat-containing protein"
                     /protein_id="HGS0771648.1"
                     /translation="MENLTNFYEKYRVYLTRPRLELLAVVTIVFCAVLVFFLNIPGKG
                     VLKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQK"
     gene            36945..39596
                     /gene="adhE"
                     /locus_tag="ACL73L_000629"
                     /gene_synonym="adhC"
     CDS             36945..39596
                     /gene="adhE"
                     /locus_tag="ACL73L_000629"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HGS0771649.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLDQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSAHA
                     ARIVRDAAIAAGAPENCVQWITQPSMEATSALMNHEGVATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKTRQMVEFNGLGHSAAIH
                     TADEELTKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGFVAADTGMDVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTIHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARILGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRELAFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
BASE COUNT        12114 a         7116 c         8950 g        11628 t
ORIGIN      
        1 tatattttgg aagggtagcg aagaggctaa acgcggcgga ctgtaaatcc gctccttcgg
       61 gttcgggggt tcgaatccct ccccttccat tttacgggca tagtttaaag gtagaactaa
      121 ggtctccaaa accttcagtg tgggttcaat tcctactgcc cgtgttaata gaattatggc
      181 gggtgtggtg aagtggttaa cacaccagat tgtggctctg gcatgcgtgg gttcgatccc
      241 catcactcgc ctattttata ttggggtata gccaagcggt aaggcaaggg actttgactc
      301 cctcatgcgt tggttcgaat ccagctaccc cagttactat ttgccggcgt ggcggaattg
      361 gcagacgcgc tggactcaaa atccagtgtc cgcaaggacg tgccggttcg accccggccg
      421 ccggtatagt atagtgttag gaacgttgtt attcttcgtt ccttttttat attatttttg
      481 gtataattat agttattcaa attttattta gattaagaaa gtgtagggga gtatgtcttg
      541 ttctatcgat ttattaaaac atcggtattt gaaaaatatt aaagaaaatc ctgaattgtt
      601 tgtcggaatt gagttggagt atcctgttgc aagtttagaa ggggatgcta cagatgttga
      661 agttatgaag gatctatttc attatttagt ttctactttg gatctcaccg tagcaaaggt
      721 agatgatttt ggcaatctga tccagttagt agatccgata agtcaggatg ctattttatt
      781 tgaagtttcc tatacaacga ttgagtttgc atttggtaag gctgaaacga ttcaagaggt
      841 cgaaaatcgt ttcaataatt atatgaatgt aattcagaga aagttagctg aatcaaatca
      901 tgctattgtt ggctgtggta tccatcccaa ctgggataaa aatgagaatt gtccagtggc
      961 ttatccacgc tatcagatgt tgatggatta tttgaatttg agtagaaata ttattaaatc
     1021 agatttacat catttccctg aatatggtac ttttatctgt gggagccagg ttcagctgga
     1081 tatttcaaaa accaactact tacgggtgat taatgctttt actcaaattg aagcggctaa
     1141 ggcttattta tttgcaaact ctgaattttc gggtgcggat tgggatacga aaatttcaag
     1201 ggatattttc tgggaagaat ctatgcatgg tatctatcca gagaatgttg gggtcaatgc
     1261 tagactcctt aatgatgaaa ctgatttttt tgactatcta aatcattctg cgatttttac
     1321 tgcggaacgt gatgggcaga cctattattt ttatcctatt caggctgggg actatttggc
     1381 tacgtccgaa atccaagcat ttgctctgaa tggggatgag gttattattt acccccaaga
     1441 gaaggatttt gaaactcatc gtagttacca gtaccaagat ttaacgactc gaggaacagt
     1501 tgagtttcgt agtgtgtgta cacagccact tgataggact tttgcttctg cagcttttca
     1561 cttgggatta ttggttaatt tagacaagtt agaagcttac ttagaaacag cacctttctt
     1621 taaagtattt ggttatgatt acaagtcttt aaggagacaa ttttctaaga aaaatcttac
     1681 agatgaggaa gaaactacga ttattgaatt ttccaaagac ttactcctac tagctgagga
     1741 gggactagtg gtgagaaata aggaagaaat gacctattta cagcctttga gagaagaatt
     1801 gagcctataa tttctcttat aaagggagaa ttttctgaaa aatcatgata taatggacga
     1861 gactatagat aaaggataga gagtaatgac attagtttat caatcaacgc gtgatgccaa
     1921 caatacagta actgccagcc aagcaatttt gcaaggtttg gcgacggacg gcggtttgtt
     1981 tacaccggat acttatccaa aggtagattt gaactttgac aaattgaaag atgcttctta
     2041 ccaggaagtt gctaagctag ttttgtcagc atttttagat gactttacag ttgaggagtt
     2101 ggactactgt atcaacaatg cctacgatag caaatttgat actccagcta ttgcaccatt
     2161 agtgaaatta gatgggcaat acaatttgga acttttccat ggttcaacga ttgcctttaa
     2221 ggatatggcc ttgtctattt tgccatactt tatgacgact gctgctaaga aacatggttt
     2281 ggagaacaag attgttatct tgacagcgac atctggtgac acggggaaag ctgctatggc
     2341 ggggtttgcg gatgtgcctg gtactgagat tatcgtcttt tatccaaagg gtggtgtcag
     2401 caaggtacaa gagttgcaaa tgaccactca gaatggtgac aatactcatg ttatcgctat
     2461 agatggtaac tttgacgatg cgcaaaccaa cgtgaagcac atgtttaacg acgtggctct
     2521 tcgtgaaaga ttagcggcca acaagttgca attttcatct gctaactcta tgaacattgg
     2581 tcgtctggta ccacagattg tttattatgt ttatgcctac gctcagttgg tcaagactgg
     2641 tgagattgtg gctggcgata aggtcaactt cacagtacca acaggaaact ttggaaatat
     2701 cttggctgcc ttttatgcca aacaaatcgg tctcccagtt ggtaaattga tctgtgcttc
     2761 aaatgacaac aatgttttga cagacttctt caagacacgt gtttacgaca agaaacgtga
     2821 gtttaaggta acaaccagcc catctatgga tatcttggta tcttcaaact tggagcgttt
     2881 gattttccat cttttgggga atgatgcggt taagacggct aaactcatga atgctttgaa
     2941 tagccaaggt caatatgagt tgactaattt tgatgcagag attttggacc tctttgcagc
     3001 tgaatatgcg actgaggaag aaactgcggc agaaatcaag cgtgtttatg agtcagattc
     3061 ttatatcgag gatcctcata cagcggttgc ctcagcagtg tatagaaaat accaatcggc
     3121 cactggagat gtaactaaga cagtgattgc ttcaacagct agtccataca agttcccagt
     3181 agttgcagta gaaactgtaa ctggaaaagc aggtttaaca gactttgaag ccttggctca
     3241 attacatgaa atctcaggcg ttgcagtgcc accagcagtt gatgggcttg aaatagctcc
     3301 aattcgtcac aagacaacag tggcagctgc tgacatgaaa gcagcggttg aggcttattt
     3361 aggactttaa gacagaggga gcaaactcgg ttgggaaacc aactgagttt cttttcatca
     3421 ggaggagaga ttgtttaaga aaaataaaga cattcttaat attgcattgc cagctatggg
     3481 tgaaaacttt ttgcagatgc taatgggaat ggtggacagt tatttggttg ctcatttagg
     3541 attgatagct atttcagggg tttcagtagc tggtaatatt atcaccattt atcaggcgat
     3601 tttcatcgct ctgggagctg ctatttccag tgttatttca aaaagcatag ggcagaaaga
     3661 ccagtcgaag ttggcctatc atgtgactga ggcgttgaag attaccttac tattaagttt
     3721 ccttttagga tttttgtcca tcttcgctgg gaaagagatg ataggacttt tggggacgga
     3781 gagggatgta gctgagagtg gtggactgta tctatctttg gtaggcggat cgattgttct
     3841 cttaggttta atgactagtc tgggagcctt gattcgtgca acgcataatc cacgtctgcc
     3901 tctctatgtt agttttttat ccaatgcctt gaatattctt ttttcaagtc tagctatttt
     3961 tgttctggat atggggatag ctggtgttgc ttgggggaca attctgtctc gtttggttgg
     4021 tcttgtgatt ttgtggtcac aattaaaact gccttatggg aagccaactt ttggtttaga
     4081 taaggaactg ttgaccttgg ctttaccagc agctggagag cgacttatga tgagggctgg
     4141 agatgtagtg atcattgcct tggtcgtttc ttttgggacg gaggcagttg ctgggaatgc
     4201 aatcggagaa gtcttgaccc agtttaacta tatgcctgcc tttggcgtcg ctacggcaac
     4261 ggttatgctg ttggcccgag cagttggaga ggatgattgg aaaagagttg ctagtttgag
     4321 taaacaaacc ttttggcttt ctctgttcct catgttgccc ctgtccttta gtatatatgt
     4381 cttgggtgta ccattaactc atctctatac gactgattct ctagcggtgg aggctagtgt
     4441 tctagtgaca ctgttttcac tacttggaac ccctatgacg acaggaacag tcatctatac
     4501 ggcagtctgg cagggattag gaaatgcacg cctccctttt tatgcgacaa gtataggcat
     4561 gtggtgtatc cgcattggaa caggatatct gctggggatt gtgcttggtt ggggcttgcc
     4621 tggtatttgg gcagggtctc tcttggataa tggttttcgc tggttatttc tacgctatcg
     4681 ttaccagcgc tatatgagct tgaaaggata ggaaatgcaa aaaacagctt ttatttggga
     4741 tttagacggg actttattgg actcttacga agcgatttta tcagggattg aggagacttt
     4801 tgctcagttt tctattcctt atgataagga gaaggtgaga gagtttatct tcaagtattc
     4861 ggtgcaagat ttgcttgtgc gggtggcaga agatagaaat ctggatgttg aggtgctaag
     4921 tcaggtgcgt gcccagagtc tgactgagaa gaatgctcag gtagttttga tgccaggtgc
     4981 gcgtgaggtg ctagcttggg cagacgaatc aggaattcag cagtttatat atactcataa
     5041 ggggaacaac gcttttacca ttctcaagga cttgggggtg gaatcctatt ttacagagat
     5101 tttaaccagt cagagtggct ttgtgcggaa gccaagtcca gaagcggcta cctatctgct
     5161 agataagtat cagttgaatt ctgataatac ttattatata ggggatcgga ctctggatgt
     5221 ggaatttgcc cagaatagtg ggattcaaag tatcaacttt ttagagtcta cttatgaagg
     5281 gaatcacagg attcaagcgt tagcagatat ttcccgtatt tttgagacta agtgataaaa
     5341 agattgtgtc agttttgtga cagagaccta acaaactatt tcaagtaacc tagtttgtta
     5401 caaggaatag acagttctgt taaataggcc cgagagggct ttttttctac attttttgtg
     5461 ttatcataga caggtactca tttgaaagga atttgaaaga atgaagaaaa gaatgttatt
     5521 agcgtcaaca gtagccttgt catttgcccc agtattggca actcaagcag aagaagttct
     5581 ttggactgca cgtagtgttg agcaaatcca aaacgatttg actaaaacgg acaacaaaac
     5641 aagttatacc gtacagtatg gtgatacttt gagcaccatt gcagaagcct tgggtgtaga
     5701 tgtcacagtg cttgcgaatc tgaacaaaat cactaatatg gacttgattt tcccagaaac
     5761 tgttttgaca acgactgtca atgaagcaga agaagtaaca gaagttgaaa tccaaacacc
     5821 tcaagcagac tctagtgaag aagtgacaac tgcgacagca gatttgacca ctaatcaagt
     5881 gaccgttgat gatcaaactg ttcaggttgc agacctttct caaccaattg cagaagctcc
     5941 aaaagaagta gcatcaagtt cagaagttac aaagacagtg attgcttctg aagaagtggc
     6001 accatctacg ggcacttctg tcccagagga gcaaacggcc gaaacaactc gcccagttga
     6061 agaagcaact cctcaggaaa cgactccagc tgagaagcag gaaacacaag caagccctca
     6121 agctgcatca gcagtggaag taactacaac aagttcagaa gcaaaagaag tagcatcatc
     6181 aaatggagct acagcagcag tttctactta tcaaccagaa gagacgaaaa caatttcaac
     6241 aacttacgag gctccagctg cgcccgatta tgctggactg gcagtagcga aatctgaaaa
     6301 tgcaggtctt caaccacaaa cagctgcctt taaagaagaa attgctaact tgtttggcat
     6361 tacatccttt agtggttatc gtccaggaga cagtggagat cacggaaaag gtttggctat
     6421 cgactttatg gtaccagaaa gttcagaatt aggggataag attgcggaat atgctattca
     6481 aaacatggcc agccgtggca ttagttacat catctggaaa caacgtttct atgctccatt
     6541 cgatagcaaa tatgggccag ctaacacttg gaacccaatg ccagaccgtg gtagtgtgac
     6601 agaaaatcac tatgaccacg ttcacgtttc aatgaatgga taaacccgac ttggtaacat
     6661 cattttgacg aatgagatct agctttcgtg ataggaagcg attctcgttc gttttttctt
     6721 tgtcatactc ttcgaaaatc tcttcaaaca acgtcagttt tatctgaaac ctcaaagctg
     6781 tgctttgagc aacctgcgac tagcttccta gtttgctttt tgattttcat tgagtatcaa
     6841 tttgaatgga aaatggaaag ttatcatctt gtaatgagtt aagcaacatt cttgcaatct
     6901 attttatagt agattgaaac tagaatagta cacaactgct tctaaaacat tgttagaaat
     6961 cgatttgact gtcctgatcg atttgtcctg ctcttatttc atttcactat actttatatt
     7021 ataattgact ggtcaaatat tgataaatca ataaaaagag aggggaagaa atgctagaga
     7081 ttcaagattt actgtatcaa ctccgcttgt ctgagcaagt gagtacgcaa ttgtttgaaa
     7141 aagcttggga ttagtttgac acggtatcag attttactgt ttttgctgga gcattctctt
     7201 tgtaaccaaa tggcggttca ggagcgtttg aaaattgatc aggctgcttt gacacggcat
     7261 ttcaagattt tggaaacgga aggtttggtg gagcgtcatc gtaatcctga aaatcagcgg
     7321 gaagtgttgg tagaggctgc gaagtatgcc aaggagcagt tagtggtgaa tccccctctg
     7381 caacatatca aggttaagga agagatggaa agtatcttaa cagagtttga gagaacagaa
     7441 ctcagccgtt tattaaataa attggttttg ggtattgaaa atatagaaat ttaaggagaa
     7501 atagatgtca attattttaa caacgatcgt tgctttggag catttttaca ttttttattt
     7561 ggaaagtatt gccacgcaat cagatgcgac tagtcgtgta tttaatatgg aaaaggaaga
     7621 attggctcat ccgtcagtaa gttcattgtt caaaaatcaa ggaatttata aggatctgct
     7681 aggagtcttt ctcttgtatg gcatttattt ctcacagaat ttagaaattg tgactatttt
     7741 tgtcttattt gtgattggtg ctgcgactta cggctcttta acagcggata aaaaaattat
     7801 tttgaaacaa ggtggatcag ctattttggc cttgattagt attttactct ttaaatacac
     7861 ttgaaggtcg attctaatct cgctaatcct tttcaatcca gaataaggga aatatgttat
     7921 acttgttttt aagaaaaaag tctcattgaa ttggttttga ggagttagaa atgaaagtat
     7981 tagtgacagg ttttgagccc tttggagggg aaaagggcaa tccagctttg gaggccatta
     8041 aaggtttacc agctgaaatc catggtgctg aggtccgttg gctagaggtg ccgacagttc
     8101 ttcacaaatc tgctcaagta ttggaagaag agatgaatcg ttatcaacct gactttgtcc
     8161 tttgtattgg gcaagctggt ggaagaacta gtttgacacc tgaacgagtg gccattaatc
     8221 aagacgatgc acgtacttct gataacgaag ataatcaacc gattgaccgt cccattcgcc
     8281 cagatggtgc ttcggcctac tttagtagtt tgccgattaa agcgatggtt caagctataa
     8341 aaaagaagga ttgccggcct ctgtttccaa tacggcaggg acttttgtct gcagccattt
     8401 gatgtatcag gctctctatt tggtagaaaa gaaatttcca tatgttaagg caggttttat
     8461 gcatattcct tatatgatgg aacaggtggt gaacagaccg cctattccag ctatgagttt
     8521 agtggatatt cggcgaggga tagaagcagc aatcggcgct atgatagaac atggagatca
     8581 ggaactcaag ttggtaggcg gagaaactca ttgatagaaa aaagcttgag gggaaaaacc
     8641 ttcaagcttt tggacgtttt cgagccaata ctgctcggta aaacataatt ttagtgcatt
     8701 ggatataagg taggagtgaa aaactagcaa tgccaaaggt aatccaattg aggaagtacc
     8761 aaggaagaag ctgtaaatct aggacaaagt gctggaactt gtagcccttc ataaaggaac
     8821 ggctagtttt taggattcgt cttggtggga cctgtcctag gtctagacta taacagagaa
     8881 gaaattccac ctgtgaatag gcataatact gtggaatata gaggatattc cctacaatga
     8941 tcaagatgag acttgcaaga aagtagagtc caaagaccat gaggaaacgc tcggtttcaa
     9001 ctgatgagag atctagattt ggaaactcag gatgtagggt gacgaatttt ttggctaaaa
     9061 agctactata aaagaggagg taaatcccaa gtaaattagg gatactccat aaaaagagat
     9121 agaaacgttt gagaagtagg gtcaaaaagg tttgagaaaa gcgctcctca tcaaagagag
     9181 ctaggctgtt ttttacagat ggctccgttt tagaatcttt catgagtgtc agtgttgcat
     9241 agacggaact ggtcaaaaga atagtcccga taaaggagac tagtagagga aagaggtagg
     9301 cttgaagtat ttggccaagt atgctgaaaa atggctgttc taaaacagtc ccgtggatcc
     9361 gagataaggg attaagaaaa ctagataaga tgaccagcat actgggaagg atatagagga
     9421 gaaagagacg gggggtgtca gcctgaaaat gttttgactc ctgacgaatt gtttttaaat
     9481 caatttttgg atagttcatt ctcttattat accatagttc ttatacatag ttcgtgacag
     9541 ttcctacttt ttttgataaa atcatacagt gtgtccttgg gcacactgta tgaactggga
     9601 ctgtctttcc cagcttcgga ggtaaaaaat gtcagattca ccaatcaaat atcgtttgat
     9661 taagaaagaa aaacacacag gagctcgtct aggagaaatc atcactcccc acggtacctt
     9721 tccgacacct atgtttatgc cagttgggac acaagccact gtcaaaactc agtcacctga
     9781 agaattgaag gagatgggtt cgggaattat cctatcaaac acctatcatc tctggcttcg
     9841 ccctggagat gaactcattg cacgcgctgg tggtctccac aagttcatga attgggacca
     9901 gcctatcttg acagatagtg gtggttttca ggtttattct ttagcagata gccgtaatat
     9961 cacagaagaa ggagtaacct ttaaaaatca tctaaatggt tctaagatgt tcctatcccc
    10021 agaaaaagcc atctctattc agaataatct gggttcagac atcatgatgt cctttgatga
    10081 atgtcctcag ttttatcaac cttatgacta cgttaagaaa tcgatcgagc gtaccagccg
    10141 ttgggctgag cgtggtttga aggctcaccg tcgtccacat gaccaaggtt tgtttggaat
    10201 tgtgcaaggt gcaggatttg aagaccttcg ccgccaatca gctcatgatc ttgtcagcat
    10261 ggatttctca ggctactcta tcggtggttt ggcagtggga gaaacccatg aagagatgaa
    10321 tgcggtcttg gactttacaa ctcaactgct acctgaaaat aaacctcgtt atctgatggg
    10381 tgtgggagcg ccagatagct tgatcgatgg ggtcattcgt ggggtggata tgtttgactg
    10441 tgtcttaccg actcgaattg ctcgtaacgg gacttgtatg accagtcaag gacgtttggt
    10501 tgtgaaaaat gcccagtttg ctgaggactt tacgccactg gatcctgagt gtgactgcta
    10561 cacatgtaat aactatacac gcgcttacct tcgtcacctg ctcaaggctg atgaaacctt
    10621 tggtatccgc ttgactagct accacaatct ttacttcttg cttaacctga tgaagcaagt
    10681 gcgacaagcc atcatggatg acaatctctt ggaattccgt gagtattttg tggaaaaata
    10741 tggctataat aagtcaggac gtaatttcta aaatggaatt gatataaaaa aatcctaagt
    10801 tttctcttag gatttttctt ctttttttga tagaataaag tgtacaatga aaggaagaat
    10861 aaactcgtat gcgcattaaa tggttttcct tgattaggat tataggttta cttttggtac
    10921 tcttgtatca cttctttcag acgatctttc ctggaggatt tttcggggta gatgtctttt
    10981 tcacattttc aggtttcctg attacagctc tactcattga agaattttct aagaacaatg
    11041 agattgattt gataggattt tttagaagac gcttttatcg gattgtgcca cctgtggttt
    11101 tgatggtctt ggtgaccatg ccttttactt tcttggttcg ccaagactat gttgctggaa
    11161 ttggtggcca gattgcgggc gtcttaggct ttatgaccaa cttctatgaa ctcctaacag
    11221 gtgggagtta tgaatctcag ttcattcctc atttgtttgt tcataattgg agtttggcag
    11281 ttgaggttca ctactatatt ctttggggat tggcagtttg gttcttatcc aaacaagcta
    11341 aatcaaatgg tcagttgaag gggatggtct ttctcttatc tgctgctgcc ttcttgatca
    11401 gtttcttctc catgtttatt ggtagttttc tagtgacctc ttattcctct gtttatttct
    11461 ccagtttaac tcatgtctat ccattctttt tgggaagtat gttagcaact attgtaggcg
    11521 ttcgtcagac gacttccctc gtcaagcagt tggataaaat ctgggattta cgaaagactt
    11581 tggtagtttt tggaggaggc tttggtttcc tagttctttt gactttcttt gtcaaattca
    11641 cttatctttt tgcctatctt atcggcttct tgcttgccag tcttgcagct cttgccatga
    11701 ttctggcggc gcgtgtctta catgaaaaga cacatcatat acaggagccg aagattatca
    11761 gctttttagc ggatactagc tatgcggttt atcttttcca ttggccttcc tatatcattt
    11821 tctcacagtt gacatcaaat cttcttgctg tattactgac tctgatttgt tcttatggct
    11881 ttgccagtct gtcattctat gtattggaac cttggattgc aggcaagaac acacctattt
    11941 tacaaactct tcgtcccctg ccttatattc acgcaattct tgcagcaggt acaggaatct
    12001 tgaccttcat tgtctgcatg gtgaccttgt tggcaccaca agtgggagcg tttgagacag
    12061 acttgactgt taatggcttg aagcaagctg caacaaatat tggccagacc aaggtgatgg
    12121 cagaacgggc agatgcaaac agtttgggaa ttgctgatgg cactatgtta attggtgact
    12181 cagtggcttt aagggcaaat acagcactac agacagctct tcctggagca cagattaacg
    12241 cgcaggtcag cgtaacaacc aagaccgcaa atgaaatcat gctaaataat agccagaata
    12301 aatttttacc taagacggtg gtcattgcga ctggggtaaa taatcctgag aattacaagg
    12361 atgactggga cagtatcgtg aaaaatcttc ctaagggaca ccgtatgatt ttggtgactc
    12421 cttatgaggg agataagaca aaagagacct atgccatcgt tgagaaggct gctgcctata
    12481 tgagagaatt ggcagagaag acaccttaca ttacgatagc agattggaat caagttgcga
    12541 aagagcatcc agaaatttgg gcaggaacag accaggttca tttcgggagt gagagtagca
    12601 ctatcgaagc aggagcaaaa ttgtatgcag atacgattgc cacagctttg cagacagctc
    12661 aagacaagcc ggttaaatca aaataacttg tattaaaaaa gagaagagtt ggagaaatcc
    12721 agctctttta aaatatctcc aaaaaatagg tctataccat ttacaaataa aaaagaaagg
    12781 tttataatgt aattgacata ataaattgta gaatcaatct tttaaggagg ttaacattat
    12841 gcctaactat attaaagcgg atcagttttt ctacccacac ggagttcgtc gaggtggtta
    12901 cttggaactt gtggacggca agtttgggaa acatgtagag cagattcctg aaggggctga
    12961 ggtgattgac tatacaggtt atagcattgc cccaggtctt gtggatactc atattcatgg
    13021 atatgcaggt gtagatgtga tggacaacaa cattgaaggt acattgcata ctatgagtga
    13081 aggacttctt agtaccggtg ttaccagttt cttacccaca actttaacag ccacttatga
    13141 gcaattgctt gcagtcactg aaaatcttgg aaaccattat aaagaagcaa caggtgctaa
    13201 gattcgtggg atttattatg aaggtccata tttcacagaa acttttaagg gggcacaaaa
    13261 tccaacttat atgagagacc cgggtgttga ggagtttcat tcttggcaaa aagcggcaaa
    13321 tggcttgctt aataaaattg cccttgcacc agaacgtgat ggggtggaag actttgttcg
    13381 tacagttacg ggcgaaggtg tgacggttgc tcttggacat tcaaacgcga cttttgatga
    13441 agccaaaaaa gcagtcgatg ctggagcgag tgtttgggtg catgcctaca atggaatgcg
    13501 tgggttgact caccgtgaat tgggtatggt tggagccatg taccaattgc cacataccta
    13561 tgcagagttg atctgtgatg gtcaccacgt agatccaaag gcttgcgaaa ttcttatcaa
    13621 acaaaaagga acagaaaaca tcgctcttat cacagactgt atgacagctg ggggattgga
    13681 agacggagat tatatgttgg gagaattccc agttgtcgtt gcaaatggaa ctgcacgcct
    13741 caaatcgaca ggtaacttgg caggttctat cctcaaactc aaagatggtt tgaagaatgt
    13801 ggtcgaatgg ggaattgcga atccgcatga agcagtcatg atggccagct tcaacccagc
    13861 taaatccgtt cacatcgatg acgtctgtgg ccaaatccgt gaaggctacg acgctgactt
    13921 catcgtatta gataaagatt tggaattggt agcaacctac ctagatggtg tgaaacgtta
    13981 tcaagcataa gagagaaggc agaagttaga gactagcttc tgctttttta tacagggtac
    14041 tttactggta aataaaaagt taaaaaaatc acaaaaaaag cttgaagaaa caaagaaaaa
    14101 ggcttacaat tgaactataa atattacaaa taaaaaaacg gaggagtgct ttatgaaagc
    14161 ctatacttat gttaaaccag gacttgcttc ttttgttgat gtagacaaac cagttattcg
    14221 caagccaaca gacgctattg tgcgtattgt aaaaaccact atttgtggaa cagacctcca
    14281 tattatcaaa ggggatgttc ctacttgcca aagtggtacc attcttggcc acgaagggat
    14341 tgggattgtt gaagaagttg gggaaggagt ttccaacttc aaaaaaggtg acaaggtctt
    14401 gatttcttgc gtctgtgcct gtggtaaatg ctactactgt aaaaaaggaa tttatgccca
    14461 ctgtgaagac gaagggggct ggattttcgg tcacttgatt gatggtatgc aggctgaata
    14521 tctacgtgtc cctcatgcag ataatactct ttaccatact ccagaagact tgtcagatga
    14581 agctttggtt atgctgtcag acattctgcc tactggatat gaaattggtg tcttaaaagg
    14641 gaaagtagaa cctggttgca gcgtagccat tattggttca ggtccagttg gattggctgc
    14701 tcttttaaca gcccaattct attcaccagc taaattgatt atggtagacc tagacgataa
    14761 ccgcttggaa actgccctat cattcggtgc gactcataag gttaattctt cagaccctaa
    14821 aaaagccatt aaagaaattt atgatttgac agatggtcgt ggtgtggatg tcgctatcga
    14881 agctgtaggg attcctgaaa catttgattt ctgtcaaaag attatcggtg tagacggaac
    14941 ggttgccaac tgtggtgtgc atggtaaacc agttgaattc gatttagata aactttggat
    15001 tcgcaacatc aatgtaacaa ctggtttggt atctacaaat acgactccac aattgttgaa
    15061 agcacttgaa agtcataaga ttgaaccgga aaaattggta actcactatt tcaaactcag
    15121 tgaaattgaa aaagcctacg aagtcttcag taaggcagca gaccaccatg ccattaaggt
    15181 cattatcgaa aacgatatct cagaagccta agtagtaaaa atatttttgt acataagcaa
    15241 atagaaattc agtcatccat cagatggctg gattttttat caaaaaatta agaaatgagc
    15301 atatttcttt ccttgtctgg cggaattggt tataatatac ggtacaaagg aatgaatgaa
    15361 tatgtatcgt gttatagaaa tgtacggaga ttttgaaccg tggtggttct tagaaggttg
    15421 ggaagaagat attgtagcaa gtagaaaatt tgaccagtat tatgatgctc tcaaatacta
    15481 caaaacttgc tggtttagat tggaacaaga atcccctctt tataaaagta gaagcgactt
    15541 gatgaccatt ttttgggacc cggaagacca acgctggtgt gatgaatgtg atgagtattt
    15601 acaacaatac cattctttgg ctcttttgca ggatgagcag gttatcccag acgaaaaact
    15661 acgctcaggc tatgaaaaac aaaccagtca ggaaaggaat cgttcttgcc gtatgaaatt
    15721 aaaatagaga aaagtaactt ttttggagtt gcttttttta tttttctaac tctttgcgaa
    15781 tagtataggt gaggaggtaa gtatggttca agaaattgca caagaaatca ttcgttcagc
    15841 tcggaaaaaa gggacgcagg atatttattt tgtccctaag ttagatgcct atgagcttca
    15901 tatgagggta ggagacgagc gctgtaaaat tggtagctat gattttgaaa agtttgcagc
    15961 cgttatcagt cactttaagt ttgtggcggg tatgaatgtg ggagaaaaaa gacgtagtca
    16021 actgggttcc tgtgattatg cctatgacca gaagatagcg tctctacgtt tatctactgt
    16081 aggcgattat cgggggcatg agagtttggt tatccgtttg ttgcacgatg aggagcagga
    16141 cttgcatttt tggtttcagg atattgaaga attaggcaag cagtacaggc aacggggact
    16201 ctatcttttt gctggtccgg ttgggagtgg taagacgacc ttgatgcatg aattgtccaa
    16261 gtcactcttt aaaggacagc aagttatgtc catcgaagat cctgtcgaaa tcaagcagga
    16321 cgacatgctt cagttgcagt tgaacgaagc aatcggccta acctatgaaa atctaatcaa
    16381 actttccttg cgtcatcgac cagatctctt gattatcgga gaaattcgtg acagcgagac
    16441 ggcgcgtgca gtggtcagag ctagtttgac aggtgcgaca gtcttttcaa ccattcacgc
    16501 caagagtatc cgaggtgttt atgagcgtct gctggagttg ggtgtgagtg aagaagaatt
    16561 ggcagttgtt ctgcaaggag tctgctacca gagattaatc gggggaggag gaatcgttga
    16621 ctttgcaaac agagattatc aagaacacca agcagccaag tggaatgagc aaattgacca
    16681 gcttcttaaa gatggacata tcacaagtct tcaggctgag acggaaaaaa ttagctacag
    16741 ctaagcaaaa aaatatcatc accctattta acaatctctt ttctagcggt tttcatctgg
    16801 tggagactat ctccttttta gataggagtt ccttgttgga caagcagtgt gtgacccaga
    16861 tgcgtacagg cttgtctcag gggaaatcat tctcagaaat gatggaaagt ttgggatgtt
    16921 caagtaccat tgtcactcag ttatctctag ctgaagttca tggcaatctt cacctgagtt
    16981 tgggaaagat agaagaatat ctagacaatc tggccaaggt caagaaaaag ttaattgaag
    17041 tagcgaccta tcctttgatt ttgctgggtt ttcttctctt aattatgctg gggctacgga
    17101 attacctgct cccacaactg gatagtagca atattgccac ccgaattatc ggtaatctgc
    17161 cacaaatttt tctaggcatg gtagggcttg tttccgtgct tgccctttta gcactaactt
    17221 tttataaaag aagttctaag atgagtgtct tttctatctt agcacgcctt ccctttattg
    17281 gaatctttgt gcagacctac ttgacagcct attatgcacg tgaatggggg aatatgattt
    17341 cacagggaat ggagctgacg cagatttttc aaatgatgca ggaacaaggt tcccagctct
    17401 ttaaagaaat cggtcaagat ctggctcaaa ccctgaaaaa tggccgtgaa ttttctcaga
    17461 cgataggaac ctatcctttc tttaggaagg aattgagtct catcatagag tatggggaag
    17521 ttaagtccaa gctgggtagt gagttggaaa tctatgctga aaaaacttgg gaagcctttt
    17581 ttacccgagt caaccgcacc atgaatttgg tgcagccact ggtttttatc tttgtggcac
    17641 tgattatcgt tttactttat gcggcaatgc tcatgcccat gtatcaaaat atggaggtaa
    17701 atttttaaaa tgaaaaaaat gatgacattc ttgaaaaaag ctaaggttaa agcttttaca
    17761 ttggtggaga tgttagtagt cttgctgatt atcagcgtgc ttttcttgct ctttgtacct
    17821 aatctgacca agcaaaaaga agcagtcaat gacaaaggaa aagcagctgt tgttaaggtg
    17881 gtggaaagcc aggcagaact ttatagctta gaaaagaatg aagatgctag cctaagaaag
    17941 ttacaagcag atgggcgcat cacggaagaa caggttaaag cttataatga atactatact
    18001 aaaaatggag gggcaaatcg taaagtcaat gattaaggcc tttaccatgc tggaaagtct
    18061 cttggttttg ggacttgtga gtatccttgc cttgggctta tccggctctg tccagtccac
    18121 ttttgcagca gtagaggagc agattttctt tatggagttt gaagaactct atcgggaaac
    18181 ccaaaaacgc agtgtagcca gtcagcaaaa gactagtcta aatttagatg ggcagacgct
    18241 tagcaatggc agtcaaaagt tgacagttcc taaaggaatt caggcaccat caggccaaag
    18301 tattacattt gaccgagctg ggggcaattc gtccctggct aaggttgaat ttcagaccag
    18361 taaaggagcg attcgctatc aattatatct aggaaatgga aaaattaaac gcattaagga
    18421 aacaaaaaat tagggcagtg attttactgg aagcagtagt cgctctagct atctttgcca
    18481 gcattgcgac cctccttttg ggacaaattc aaaaaaatag gcaagaggaa gcaaaaatct
    18541 tgcaaaagga agaagtcttg agggtagcta agatggcctt gcagacaggt caaaatcagg
    18601 taaacataaa cggagttgag attcaggtgt tttctagcga aaagggattg gaggtctacc
    18661 atggttcaga acagttgttg gcaatcaaag agctataagg tcaaggcttt taccttgtta
    18721 gaatccctgc ttgccctgct tgtcatcagt gggggcttac tcctttttca agctatgagt
    18781 cagctcctca tttcagaagt tcgctaccag caacaaagcg agcaaaagga gtggctcttg
    18841 tttgtggacc aacttgaggt agaattagac cgttcgcagt tcgaaaaagt agaaggcaat
    18901 cgcctataca tgaagcaaga tggcaaggac atcgccatcg gtaagtcaaa gtcagacgat
    18961 tttcgtaaaa ccgatgctag tggacggggg tatcagccta tggtttatgg cctcaaatca
    19021 gctcagatta cagaagacaa tcaactggtt cgctttcatt tccagtttca aaaaggctta
    19081 gaaagggagt tcatctatcg tgtggaaaaa gaagaaagtt aaggcaggtg ttctcctcta
    19141 tgcagtcacc atggcagcta tctttagtct tttgttgcaa ttttatttga accgacaaat
    19201 cgcccactat caagactatg ctttaaataa agaaaaattg gttgcttttg ccatggctaa
    19261 acgaaccaag gataaagccg agcaagaaag tggggaacag gtctttaatc taggtcaggt
    19321 aagctatcaa aacaagaaaa caagcttggt gacgactgtt cgtacgtcta agagccaata
    19381 tgagtttctg tttccttcag tcaaaatcaa agaagagaaa agagataaaa aggaagaggt
    19441 agcgaccgat tcaagcgaaa aagtggagaa gaaaaaatca gaagagaagc ctgaaaagaa
    19501 agagaattcc tagtcaattc aactataatg cgttgaatcc agaatagtcc actgtagttt
    19561 ctagaaaatt gctggaaatg gatgttaagc tccaattcat ttgtttatat cttatttcag
    19621 tttactatac tttgtgctaa actaaagata tgaaacatga ttttaaccac aaagcagaaa
    19681 ctttcgactc ccctaaaaat atcttcctcg caaacttggt atgtcaagca gtcgagaaac
    19741 agattgatat tttatcagac aaagtaattt tagatttcgg tggtggcacg ggtctattag
    19801 ccttgcccct agccaagcag gctaagtcag ttactcttgt agacatttct gagaaaatgt
    19861 tggagtaagc tcgtttgaaa gcggagcagc aagcaatcaa gaatatccag tttttggagc
    19921 aagatttacc gaaaaatccc ttggagaaag agtttgattg ccttgctgtt agtcgggttc
    19981 ttcatcatat gcctgatttg gatgcggatc tctcactgtt tcatcaacat ttgaaggaag
    20041 atgggaaact catcattgct gattttacca agacagaagc taatcatcat ggatttgatt
    20101 tagctgaact ggaaaacaag ctaattgagc atggtttttc atctgtgcat agtcagattc
    20161 tctatagcgc tgaagacctg tttcaaggga atcactcaga attcttttta acagtatccc
    20221 aaaaatcact cgcctagtca gggagtgatt tttctataag gatggaaaaa agaagggaaa
    20281 tttgataaga taggaatatg gattttgaaa aaattgaaca agcttatatc tatttactag
    20341 agaatgtcca agtcatccaa agtgatttgg cgaccaactt ttatgacgcc ttggtggagc
    20401 aaaatagcat ctatctggat ggtgaaactg agctaaacca ggtcaaagac aacaatcagg
    20461 cccttaagcg tttagcacta cgcaaagaag aatggctcaa gacctaccag tttctcttga
    20521 tgaaggctgg gcaaacagaa cccttgcagg ccaatcacca gtttacaccg gatgctattg
    20581 ctttgctttt ggtgtttatt gtggaagagt tgtttaaaga ggaggaaatt actatcctcg
    20641 aaatgggttc tgggatggga attctaggcg ctactttctt gacctcgctt gataaaaagg
    20701 tggattactt gggaatggaa gtggatgatt tgctgattga tctggcagct agcatggcag
    20761 atgtaattgg tttgcaggct ggctttgtcc aaggagatgc cgttcgtcca caaatgctca
    20821 aagaaagcga tgtggtcatc agtgacttgc ctgtcggcta ttatcctgat gacgccgttg
    20881 cgtcgcgcca tcaagttgct tctagccaag aacatactta cgcccatcac ttgctcatgg
    20941 aacaagggct taagtacctc aagtcagacg gatacgctat ttttctagct ccgagtgatt
    21001 tgttgaccag tcctcaaagt gatttgttaa aagaatggct gaaagaagag gcgagtctgg
    21061 ttgctatgat tagtctgcct gaaaatctct ttgctaatgc taaacaatct aagactattt
    21121 ttatcctaca gaagaaaagt gaaatagcag tggagccttt tgtttatcca cttgctagct
    21181 tgcaagatgc aagtgtttta atgaaattta aagaaaattt tcaaaaatgg actcaaggta
    21241 ctgaaatata aaatagattt tgttataata gttgaaaacg cttaaaaagg ggtatcatat
    21301 tatgacaaaa acaattgcaa tcaatgcagg aagttcaagt ttgaaatggc aattatactt
    21361 aatgccagaa gaaaaagtat tggcgaaagg tttgattgaa cgtatcggtt tgaaagattc
    21421 aatttcaact gtaaaatttg acggccgttc tgaacaacaa attttggata ttgaaaatca
    21481 tatacaagcc gttaaaattt tattggatga cttgattcgt ttcgatatta tcaaggctta
    21541 tgacgagatt acaggtgttg gacatcgtgt tgttgctggt ggagaatatt tcaaagaatc
    21601 aacagttgtt gagggagatg ttttagaaaa agttgaagag ttgagtttgt tggctcctct
    21661 acacaacccg gccaatgcag caggtgttcg tgccttcaag gaattgttgc cagacattac
    21721 cagtgtagtt gtttttgata cttccttcca cacaagtatg ccagagaaag cttatcgtta
    21781 ccctctacca acaaaatatt acacagaaaa caaggttcgt aaatacggtg ctcatggtac
    21841 aagtcaccag tttgtagcag gagaagctgc aaaactcttg ggacgtccat tagaagactt
    21901 gaagttaatt acctgtcata ttggtaacgg aggctcaatt acagctgtga aagccggcaa
    21961 atctgtagac acttctatgg ggttcactcc tcttggtggt attatgatgg gaacgcgtac
    22021 aggggatatt gatccagcta tcattcctta tttaatgcaa tatacagagg attttaacac
    22081 accagaagat atcagtcgtg ttcttaaccg tgaatcaggt cttttgggag tttctgctaa
    22141 ttctagcgat atgcgcgata tagaagcagc tgtagcagaa gggaatcacg aggctagctt
    22201 ggcttatgaa atgtatgttg accgtatcca aaaatatatc ggtcagtacc ttgcagtgct
    22261 aaatggagca gatgccattg ttttcacagc aggtgtcggt gaaaatgcag agaatttccg
    22321 tcgtgatgta atctcaggga tttcgtggtt tggttgtgat gttgatgatg aaaagaatgt
    22381 ctttggcgtt acaggagaca tctcaacaga ggcagctaaa atccgtgtct tggttattcc
    22441 aacagatgaa gaattagtca ttgcccgtga cgttgaacgc ttgaaaaaat aagtgaaact
    22501 aaaaaaatat tcaatacaag gagttgggaa agttattttt ccagcttctt tttctgatga
    22561 aattgtccaa aaccttgcta tgattggctt ttttgaaaaa tatggtataa tagtagtaat
    22621 ttaatagatg gagttgagtt ttgaagaaaa actttcgtgt aaaaagagag aaagatttta
    22681 aggcgatttt caaggagggg acaagttttg ctaatcgcaa atttgtggtc taccaattag
    22741 aaaaccagaa aaaccatttt cgagtaggtc tatcagttag caaaaaactg gggaatgccg
    22801 tcactagaaa tcaaattaag cgacggattc ggcatattat ccagaatgca aaagggagtc
    22861 tggtagaaga tgtcgacttt gttgtcattg ctcgaaaagg agtcgaaacc ttgggatacg
    22921 cagagatgga gaaaaatcta ctccatgtat taaaattatc aaagatttac cgggaaggaa
    22981 atgggagtga aaaagaaact aaagttgact agtttgctag gactgtctct gttaatcatg
    23041 acagcctgtg cgactaatgg ggtaactagc gatattacag ccgaatcggc tgatttttgg
    23101 agtaaattgg tttacttctt tgcggaaatc attcgctttt tatcgtttga tattagtatc
    23161 ggagtgggga ttattctctt tacggtcttg attcgtacag tcctcttgcc agtctttcag
    23221 gtgcaaatgg tggcttctag gaaaatgcag gaagctcagc cacgcattaa ggcgcttcga
    23281 gaacaatatc caggtcgaga tatggaaagc agaaccaaac tagagcagga aatgcgtaaa
    23341 gtatttaaag aaatgggtgt cagacagtca gattctcttt ggccgatttt gattcagatg
    23401 ccggttattt tggccttgtt ccaagcccta tcaagagttg actttttaaa gacaggtcat
    23461 ttcttatgga ttaaccttgg tagtgtggat acaacccttg ttcttccgat tttagcagca
    23521 gtattcacct ttttaagtac ttggttgtcc aacaaagctt tgtctgagcg aaatggcgct
    23581 acgactgcga tgatgtatgg gattccagtc ttgattttta tctttgcagt ttatgcgcca
    23641 ggtggagtcg ccctatactg gacagtgtct aatgcttatc aagtcttgca aacctatttc
    23701 ttgaataatc cattcaagat tatcgcagag cgcgaggccg tagtacaggc acaaaaagat
    23761 ttggaaaata gaaaaagaaa agccaagaaa aaggctcaga aaacgaaata aataaggagg
    23821 aatctggtaa tggtagtatt tacaggttca actgttgaag aagcaatcca gaaaggattg
    23881 aaagaattag atattccaag aatgaaggct catatcaaag tcatttctag ggagaaaaaa
    23941 ggctttcttg gtctatttgg taaaaaacca gcccaagtgg atattgaagc gattagtgaa
    24001 acgactgttg tcaaagcaaa tcaacaggta gtaaaaggcg ttccgaaaaa aatcaatgat
    24061 ttgaacgagc ctgtgaagac ggttagtgaa gaaaccgttg accttggtca tgtagttgat
    24121 gctattaaaa aaatagagga agaaggtcaa ggtatttctg atgaagtcaa ggctgaaatc
    24181 ttaaaacatg aaagacatgc cagcactatc ttagaagaaa ctggtcacat tgagatttta
    24241 aatgaacttc aaatcgagga agcgatgagg gaagaagcag gcgctgatga ccttgaaact
    24301 gagcaagacc aagctgaaag tcaagaacta gaagacttgg gcttgaaagt tgaaacgaac
    24361 tttgatattg aacaagtagc tacggaagta atggcttatg ttcaaacgat tattgatgac
    24421 atggatgttg aggctacact ttcaaatgat tataaccgtc gtagcatcaa tttgcaaatt
    24481 gatactaacg aaccaggtcg tattatcggc taccatggta aagtcttgaa ggccttgcaa
    24541 ctgttggctc aaaattatct ttacaactgc tattcaagaa ccttctatat cactattaat
    24601 gtcaatgatt atgttgaaca ccgtgcagaa gtcttgcaga cctatgcgca aaaattggcg
    24661 attcgtgttt tagaagaagg acgtagtcat caaacagatc caatgtcgaa tagtgaacgc
    24721 aagattatcc accgtattat ttcacgtatg gatggcgtga ccagttactc tgaaggtgat
    24781 gagccaaatc gctatgtcgt tgtagataca gaataagtaa aatcagggtt gacctgattt
    24841 tttgctagct agaggaggtt aaactgatgt tgaataagat aagagactat ttagactttg
    24901 ctggtttgca gtaccgtaat cctgataaag cgggagcaga gcgagagaag atgctggcat
    24961 tccgccacaa aggacaagag gcccgaaagg cttttacaga actggccaaa gcctttcaag
    25021 caagccatcc agaatggcaa ctccaacaga ctagtcagtg gatgaatcag gcccagcgtt
    25081 tgagaccaca tttttgggtt tatctacaga gagacggaca agtgacagaa cctatgatgg
    25141 ccttacgttt gtatgggaca tctactgact ttggaatttc tttggaagtc agtttcatcg
    25201 aacgtaagaa ggatgagcaa acactgggca agcaggccaa agttttagac attccaaccg
    25261 ttaaagggat ttattatcta acctactcta atggtcaaag tcaacggtgg gaggcgaatg
    25321 aagaaaagcg tcgtacttta cgcgagaagg tgagaagtca agaagctcga aaagttttag
    25381 tgaaggtaga tgttcctatg acagaaaatt cgtctgaaga agaaatcgta gaaggcttat
    25441 tgaagtctta ttctaaaatt cttccctatt atctagctac gagaaaataa gataatttgt
    25501 aaaacatcat aaatcataca gtccaagagt gaacagtccg ctgtgtaatt cttggtcttt
    25561 ttgtttgcgc tttcgcatta tataataaac ttacaaaaac aattcaaaag gagaacaatt
    25621 atggaagtcg tttcaagtgt tctaaattgg ttttctagca atattttgca gaatcccgca
    25681 tttttcgtag gtttattggt gttgatagga tatgcacttt tgaaaaaacc tgcccatgac
    25741 gttttttcag ggtttgttaa agcaacagta gggtatatgt tgcttaacgt gggtgctggt
    25801 ggtttggtta caacctttcg tccaatctta gcagctctta actacaaatt ccaaattggt
    25861 gcagcggtta tcgaccctta ctttggactt gctgcagcaa acaacaaaat tgcagcagag
    25921 tttccagatt ttgttggaac tgcaactaca gctctattga ttggttttgg aataaatatc
    25981 ttgctcgtag ctcttcgaaa gattacgaag gtaagaaccc tctttattac tggtcacatc
    26041 atggtacaac aagctgcaac agtatctctt atggttctat tcttagtacc acaattgcgc
    26101 aatgcttacg gtacagcagc gattggtatc atctgtggac tttactgggc agttagttca
    26161 aatatgactg ttgaggcaac tcaacgcttg actggtggtg gcggatttgc gattggtcac
    26221 caacagcaat ttgcaatctg gtttgtagat aaagtagcag gacgctttgg taagaaagaa
    26281 gaaagtttag acaatcttaa attacctaag ttcctctcaa tcttccacga tacagttgtt
    26341 gcatctgcta ccttgatgct cgtattcttc ggagccattc ttttaatctt gggtccagac
    26401 attatgtcta ataaagaagt catcacttca ggaactctat tcaatcctgc taaacaagat
    26461 ttctttatgt acattatcca aacagccttt accttctcag tttacttgtt cgttttgatg
    26521 caaggtgtcc gaatgttcgt atctgagttg acaaacgcct tccaaggtat ttcaaacaaa
    26581 ttgttgccag gttcattccc agcggttgac gttgcagctt cttatggatt tggttctcca
    26641 aatgctgtct tgtcaggatt tacctttggt ttgattggtc aattgattac aattgtcttg
    26701 ctcatcgtct ttaaaaatcc gattcttatt attacaggat ttgtaccagt gttctttgac
    26761 aatgcagcca ttgcggtcta cgctgataaa cgcggcggat ggaaagcggc tgttatcctt
    26821 tcctttatat caggtgtcct tcaagttgct ctaggagctc tttgtgtggc ccttctcgat
    26881 ttggcatctt atggtggcta ccatggaaat atcgactttg aattcccatg gcttggattt
    26941 ggatatatct tcaaatacct tggtattgtt ggttatgtac ttgtgtgtct cttcttgctt
    27001 gttattcctc aacttcaatt tgccaaagca aaagataaag agaaatatta caacggtgaa
    27061 gttcaagaag aagcttagta tctagaaaag gagaaataaa atggttaaag tattagcagc
    27121 gtgcggaaat ggaatgggtt catcaatggt tatcaagatg aaggttgaaa atgctctccg
    27181 taagcttaat caaacagatt ttacagtcaa ttcatgcagt gtcggtgaag ctaaaggttt
    27241 agcagtagga tatgacatcg taatcgcttc tcttcatttg attcaagaat tggaagggcg
    27301 aactaatggg aagttaattg ggcttgataa cttgatggat gataaagaaa tcaccgaaaa
    27361 actcagtcaa gcactacagt aaaaggttgg agggggctgg acagaaactg agagttatcg
    27421 tttctgtcct tctccctctt taaataaagg aggcagatat gaatttaaaa caagctttaa
    27481 ttgacaatga ctcgatccga ctaggtttag aggctaacaa ttggaaagaa gcagtcaagg
    27541 tagcagtaga tcccttaatt gaaagtgggg caattttgcc agagtattac gatgctatca
    27601 ttgaatcgac tgaagagtat gggccttact atatcttgat gccaggtatg gctatgcccc
    27661 acgctagacc tgaagctggt gtgcaaagtg atgccttttc attgattacc ttacaaaatc
    27721 ctgttgtatt ttcagatggg aaagaggtat ctgttttgtt ggcactagca gcaacaagct
    27781 caaaaattca cacaagtgta gccattccac aaattattgc cctgtttgaa ttagacgatt
    27841 ctattgcacg tttacaggct tgccagacta aagaagatgt cttagctatg attgaagaat
    27901 ctaaggatag cccttatctc gaaggattgg acttggaaag ttagaaagag gaataaagaa
    27961 atgacaaaaa gaatacctaa tttacaagtt gcattagacc attcagactt gcaaggagcg
    28021 attaaagcag ctgtttctgt tggtcaggaa gtagatatta tcgaagctgg aactgtttgc
    28081 ttgcttcaag ttggaagtga actggctgaa gtcttgcgta gccttttccc agataagatt
    28141 attgtggcag acacaaaatg tgctgatgct ggtggaacag ttgctaaaaa taatgcggtt
    28201 cgtggagcag actggatgac ttgtatctgt tgtgcaacca tccctactat ggaagcagct
    28261 ctaaaggcta tcaagactga acgaggagaa cgaggcgaaa tccagatcga gctttatggc
    28321 gattggactt ttgaacaagc tcagctttgg ctagatgcag gtatctcaca agctatttat
    28381 caccaatctc gtgatgctct tcttgctggt gaaacttggg gtgaaaaaga ccttaataag
    28441 gttaaaaaac tcattgacat gggcttccgt gtatctgtaa caggtggtct agatgtagat
    28501 actctcaaac tctttgaagg tgttgatgtc tttaccttta tcgcaggtcg tggaattaca
    28561 gaggctgcgg atccagcagg agcagcgcgt gccttcaagg atgaaatcaa acgaatttgg
    28621 gggtaaatca tggtacgtcc aattggaatt tatgaaaagg caaccccaac acactttact
    28681 tggctagaac gtttaaattt tgccaaggag ttaggctttg attttgtcga gatgtctatt
    28741 gacgaacgtg acgagcgttt agcaagactt gactggagta aggaagaacg cttggaagtt
    28801 gtcaaagcaa tctatgaaac tggtgttcgt attccttcta tctgtttttc aggccatcgt
    28861 cgctacccat tgggttcaaa agatccagtt ctagaggaaa aatctctaga actcatgaaa
    28921 aaatgtatcg aattagctca agacttggga gttcgtacga ttcaattagc tggttacgat
    28981 gtttactatg aggaaaagtc accccagaca cgccaacgtt ttatcaaaaa tttgagaaaa
    29041 gcctgtgact gggctgaaga agctcaggtg gtacttgcta ttgaaattat ggatgatcct
    29101 ttcatcaata gcatcgaaaa atatttggct atagaaaaag agattgactc tcccttcctc
    29161 tttgtatatc cagatattgg taatgtgtct gcatggcata atgatatcta tagtgagttt
    29221 tatcttggtc atcatgccat cgcagctctc catctcaagg atacttatgc agtgacagaa
    29281 agttcaaggg gccagttccg agatgtacct tttgggcaag gttgtgtcaa atgggaagaa
    29341 gctttcgata ttttaaagga aaccaattat aatggacctt tcctaatcga aatgtggtct
    29401 gaaaattgtg aaacagtaga agaaacacgc gcagccattc aagaggcgca agcttttctc
    29461 tatccactca ttaagaaagc aggtttgatg taagatgaat caagtaatca atgctatgcg
    29521 taaacgagtc tgtgctgcca atcaatcatt gccaaaacat ggacttgtca aatttacctg
    29581 ggggaatgta tctgaagtca atcgcgaact cggtgtcatt gttatcaaac catcaggcgt
    29641 ggattatgac gaattgacac ctgaaaacat ggtagtgact gatctagatg gtaagatcct
    29701 agaaggggat ttaagaccat cttccgacct cccaactcat gtgcaattat ataaggcttg
    29761 gtcagaaatt ggtagtgtgg ttcacaccca ttcgacagaa gctgttgctt gggctcaggc
    29821 aggtcgtgat attcctttct acggaacaac ccatgcagat tatttctacg gttcaatccc
    29881 ttgcgcccgt agtttgacca aggacgaagt agaagtggcc tatgaaaaag atactggcct
    29941 ggttatcgta gaagagtttg aacatcgcgg acttaacccg gttgaagtac caggaattgt
    30001 tgtacgcaat cacggtccat tcacctgggg caaaaatcca gagaatgctg tttatcactc
    30061 tgtcgtacta gaggaagtat caaagatgaa tcgctttaca gaacaaatca atccaagagt
    30121 tgaacctgct ccccagtaca tactagaaaa acactaccaa cgtaaacatg gaccaaatgc
    30181 ttattatggt caaaagtaag aacgatgaag gaggagaaaa agataaattt agctcctctt
    30241 tttacatttg atttttattg agagtaaagt tggagttgaa gtaattttga aagatttttt
    30301 agaaatagcg cttgatatat atggtaaaat aaaaagaatt gctgtgatat caatagattt
    30361 gggggatttt ttaatatggt actggataag gcaagttgtg atttgcttca atatttgatg
    30421 gatcaagaaa cgtccaaaac gattatggcg atttcgaaag atttgaaaga gtcaagaagg
    30481 aaaatttatt atcacattga caaaatcaat gctgctctgg ttgacgaggc gcttcacatc
    30541 attagtattc cacgaattgg tattcactta acggaagagc agagagatgc ttgttgtaaa
    30601 ctattatcgg aagtagattc gtacgattat atcatgagtg cgcatgaacg tatgatgata
    30661 atgttactat ggataggtat ttctaaagaa cgtattacga ttgaaaaatt gatagagtta
    30721 acagaggtat ctaggaatac tgttctcaat gatttgaata gtattcgtta tcaactaact
    30781 ttggaacaat atcaggtgac cttgcaagtg agcaagtcac agggatacca ccttcatgcc
    30841 caccctctta ataaaattca gtatcttcaa tcgcttctat atcatatttt tatggaagaa
    30901 aatgccactt ttgtatctat tttagaagat aagatgaaag agaggttaga tgatgagtgt
    30961 ttgctttctg ttgaaatgaa ccaatttttt aaggaacagg ttcctttagt tgaacaagat
    31021 ttagggaaga aaataaacca tcatgaaata acttttatgt tgcaggttct accttatttg
    31081 ctgttaagct gtcataatgt tgaacagtat caagaaagac atcaggatat agagaaagaa
    31141 ttttctttga taagaaaaag aatagagtat caggtgtcta agaaattagg agaacggttg
    31201 tttcaaaatt ttgaaatttc tttgtcagga cttgaagttt ctcttgtagc tgttctcctc
    31261 ctctcctatc gtaaagattt ggatattcat gcagaaagtg atgattttcg gcaattaaaa
    31321 cttgctttag aagaatttat ctggtatttt gaatcacaaa tccgaatgga gattgagaac
    31381 aaggatgatt tgttacgaaa tttgatgatc cactgtaaag ccttgttatt tagaaagact
    31441 tacggtattt tttctaaaaa tcctctaaca aaacaaattc gatccaagta tggagaatta
    31501 tttttagtca ctagaaaatc tgcggaaatt ttagaaggag catggtttat tcggctaaca
    31561 gacgatgata ttgcctattt gacgattcat attggaggat ttttaaagta tacaccatcg
    31621 tctcaaaaaa atatgaaaaa agtttatctc gtttgtgatg aaggtgttgc ggtttcgaga
    31681 cttttgctga aacaatgcaa actttatttt ccaaatgagc aaattgacac tgtatttaca
    31741 acagaacaat ttaagagtgt ggaagatatt gcacaagttg atgtagtgat tactactaat
    31801 gatgatttgg atagcagatt tccgatttta agggttaatc ctatccttga agcagaagat
    31861 attttgaaaa tgctagacta tcttaaacac aatatatttc gtaataagag caaaagtttc
    31921 agtgaaaatc tttctagtct tatttcgtct tatattgtag acagcaagtt ggctagtaag
    31981 ttccaagaag aggttcaaac acttataaat caagaaatag tagttcaagc ttttttggaa
    32041 gatatttgaa ggacagtcca atgatgaaca caaacctgtg tttttcttgg tcttttttag
    32101 tgttttgaag ggtggtatac taatctcaaa gataacaatt atatccaaag gaggcaacat
    32161 atgccaaacg tcaaagaaat tacaagagag tcatggattt tagccacttt cccagagtgg
    32221 ggaacatggt tgaacgaaga aatcgaagaa gaagtcgtac ctgaaggcaa ctttgccatg
    32281 tggtggctag gcaactgtgg tacttggatt aagacaccag ctggtgctaa cgttgtcatg
    32341 gacctttggt caaaccgtgg aaaatcaacc aaaaaagtga aagatatggt tcgtgggcac
    32401 caaatggcaa atatggcagg tgttcgtaag ttgcaaccaa acttgcgtgt tcagccaatg
    32461 gttatcgatc catttgctat caacgaacta gactattact tagtttcaca cttccacagt
    32521 gatcatatcg acccatacac agctgcagca attctcaaca atcctaagtt agagcatgtt
    32581 aagtttatcg gtccttacca ctgtggacga atctgggaag gatggggtgt tccaaaagaa
    32641 cgtatcatcg ttgttaaacc aggtgacact atcgaattaa aagatatgaa gattcatgca
    32701 gtagaatcat ttgaccgtac ttgcttggta actctcccag tgaacggtgc tgatgagaca
    32761 ggcggtgaac ttgctggctt ggctgttaca gatgaagaaa tggctcaaaa ggctgttaac
    32821 tatatctttg aaacaccagg tggaaccatc tatcatggtg cagattctca cttctcaaac
    32881 tattttgcaa aacatggtaa agactttaaa attgatgttg ctttgaataa ctatggtgaa
    32941 aatccggtag gtatccaaga caaaatgaca tctatcgacc ttcttcgtat ggcagaaaat
    33001 ctgcgtacca aagtcattat cccagttcac tatgatatct ggtctaactt catggcttct
    33061 actaatgaga ttctagaact ttggaaaatg cgaaaagatc gcttgcaata cgatttccat
    33121 ccatttatct gggaagttgg cggtaagtac acttatcctc aagatcaaca cttagtagaa
    33181 taccatcatc cacgtggttt tgatgattgt tttgaacaag actctaacat tcaatttaaa
    33241 gctttgctat aatataaaaa tattctttgg aggttatccg tatgttgcat gatacggata
    33301 attttcatat aatagtaaaa tagaatgtgt gattcaataa tcacctcaaa tagaaaggaa
    33361 attctatgtc aaatctatct gttaatgcaa ttcgttttct aggtattgac gccattaata
    33421 aagccaactc aggtcatcca ggtgtggtta tgggagcggc tccgatggct tacagcctct
    33481 ttacaaaaca acttcatatc aatccagctc aaccaaactg gattaaccgc gaccgcttta
    33541 ttctttcagc aggtcatggt tcaatgctcc tttatgctct tcttcatctt tctggttttg
    33601 aagatgtcag catggatgag attaagagct tccgtcaatg gggttcaaaa acaccaggtc
    33661 acccagaatt tggtcatacg gcagggattg atgctacgac aggtcctcta gggcaaggga
    33721 tttcaactgc tactggtttt gcccaagcag aacgtttctt ggcagccgaa tataaccgtg
    33781 aaggctacaa tatctttgac cactatactt acgttatctg tggagacgga gacttgatgg
    33841 aaggtgtctc aagcgaggca gcttcatacg caggcttgca aaaacttgat aagttggttg
    33901 ttctttatga ttcaaatgat atcaacttgg atggtgagac aaaggattcc tttacagaaa
    33961 gtgttcgtga ccgttacaat gcctacggtt ggcatactgc cttggttgaa aatggaacag
    34021 acttggaagc catccatgct gctatcgaaa cagcaaaagc ttcaggcaag ccatctttaa
    34081 ttgaagtgaa gacggttatt ggatatggtt ctccaaacaa acaaggaact aatgctgtac
    34141 acggcgcccc tcttggagca gatgaaactg catcaactcg ccaagccctc ggttgggact
    34201 acgaaccatt tgaaattcca gaacaagtat atgctgattt caaagaacat gttgcagacc
    34261 gtggcgcatc agcttatcaa gcttggacta aattagttgc agattataaa gaagctcatc
    34321 cagaactggc tgcagaagta gaagccatca tcgacggacg tgatccagtc gaagtgactc
    34381 cagcagactt cccagcttta gaaaatggtt tttctcaagc aactcgtaat tcaagtcaag
    34441 atgccttgaa tgttgtggca gctaaattac caaccttcct aggtggatca gctgaccttg
    34501 ctcactcaaa catgacttat atcaagacgg atggacttca agacgatacc aatcgcttga
    34561 atcgtaacat tcagtttggt gttcgtgaat ttgcaatggg aacaatcttg aacgggatgg
    34621 cccttcatgg tggacttcgt gtatacggtg gaactttctt cgtcttctct gactatgtga
    34681 aggcagctgt ccgcttgtca gccttacaag gacttcctgt gacttatgtc tttacccatg
    34741 attcaatcgc agttggggaa gatggtccga ctcacgaacc agttgagcat ttagcaggtc
    34801 ttcgtgctat gccaaatcta aatgttttcc gtccagcaga tgcgcgtgaa acgcaagcag
    34861 cttggtacct tgcagtgaca agtgagaaaa caccaactgc ccttgtcttg acacgtcaaa
    34921 acttgactgt tgaagatgga acagacttcg acaaggttgc taaaggtgct tatgttgtat
    34981 atgaaaatgc agccgacttt gataccatct tgattgcgac aggttcagag gttaatcttg
    35041 ctgtctcagc tgctaaagaa ttggctagtc aaggcgaaaa aatccgcgta atcagcatgc
    35101 catctacaga tgtctttgat aaacaaaatg cagcttacaa ggaagagatt cttccaaatg
    35161 cagtccgtcg tcgtgttgca gtcgaaatgg gtgcaagtca aaactggtac aaatatgttg
    35221 gtctcgatgg tgccgttcta ggtattgata ccttcggagc ctctgcccca gcaccaaaag
    35281 tattggcaga atatggcttt actgtagaaa atcttgtaaa aattgttcga aacttgaaat
    35341 aatcctaaaa atcagggcgt aagctctggt ttttcttgcc agaaaagtaa ggtacaatct
    35401 tgtaaaagta gctgaaattt gatatagtag tcctatgtaa aagacaaagg agaaaacaat
    35461 gaatccaaat attacttttt taatcatgct tgtaggtatg atggccttga tgttctttat
    35521 gcaacgttct caaaagaaac aagctcaaaa gcgtatggaa agcttaaata aactacaaaa
    35581 aggctatgaa gtgattacaa tcggtggact ttatggaaca gtcgatgaag tagatacgga
    35641 gaagggaaca atagttcttg acgtagatgg agtttacttg acttttgaac tagctgctat
    35701 caagacagta ttaccgctga aagaaacagc ttcactagaa ggcgcaattg aaaaataaga
    35761 cgggatcacg aactcccgtt tttctataaa agaaaggaaa tgggatgaaa aaattagtct
    35821 ttgtctgtct gggaaatatt tgccgtagcc ctatggccga gtttgttatg aaatcaatga
    35881 cagataacta cgaaatccaa agtcgagcaa cttcctcttg ggaacatggc aatccgattc
    35941 ataaggggac tcagggaatt tttcaagagt atgagattcc ttatgacaag aacaagacat
    36001 cgcttcagat tagtaaggaa gattttgaag cctttgatta tattatcgga atggacgctt
    36061 caaatatttc tgacttacgt cagatgtgtc cagtagactg tcaagataag atttactcat
    36121 tttcatctga aagtgttcca gacccttggt atacaggaga ttttgaagaa acctatcgac
    36181 gtgttcaaga gggctgtcaa gcttggttag aacgtttaga aaaggagagt taaggtggaa
    36241 aatcttacga atttttacga aaagtatcgt gtctatctga ctcgtccacg tttagagctt
    36301 ttggcagtag ttaccattgt tttctgtgct gtactcgtct tttttctaaa tattccagga
    36361 aaaggtgtct taaaactcga taatggaacg attgtttatg atggcagtct tgtccgtggt
    36421 aaaatgaatg gccaaggtac cattaccttc caaaatggag accaatatac aggtggcttc
    36481 aacaatggag ccttcaacgg aaaaggtacc tttcaatcta aagaaggctg gacctacgaa
    36541 ggtgattttg taaatggtca ggctgaagga aaagggaaac taacaacaga acaagaagtc
    36601 gtttatgaag gaacttttaa acaaggcgtt tttcaacaaa aataaagcct ccttatcaaa
    36661 ggaggtatta ttagaatcac aaggtaagcg cttacctgta aatccctttc tttccaaatc
    36721 cctcttccaa gcaagtttgt gaaataaaaa atatttgaaa taaatttcac aaacttcaaa
    36781 gataaaactt gataagaaaa gaaaatgaga aaagtttcac aagagtttaa aaattctttg
    36841 tgaaatgttt atgaaactgt ttacaaagtt gaaataatgc gttataataa acttgtgaaa
    36901 aaattaacaa aggatatcgt tccttgaaag ctatggagga aaatatggct gataaaaaaa
    36961 ctgtgacacc agaggaaaag aaactcgttg ctgaaaaaca cgtagatgag ttggttcaaa
    37021 aagctctagt tgcccttgaa gaaatgcgta aattggatca agaacaagtt gactacatcg
    37081 ttgccaaagc atcagtagca gctttggatg cccacggaga attggcttta catgcctttg
    37141 aagaaacagg acgtggtgta tttgaagaca aagcaactaa gaacttgttt gcctgtgaac
    37201 acgtagtaaa caacatgcgc cacactaaga cagttggtgt tatcgaagaa gacgatgtaa
    37261 caggattgac tcttattgct gaaccagttg gtgttgtttg tggtattact ccaacaacaa
    37321 acccaacatc aacagcaatc ttcaaatcat tgatttcatt gaagacacgt aacccaatcg
    37381 tctttgcctt ccatccatca gcacaagaat catctgctca tgcagctcgt atcgtccgcg
    37441 atgcagctat cgcagctggt gctcctgaaa actgtgtgca atggattact caaccatcta
    37501 tggaagcaac aagtgccctt atgaaccacg aaggtgttgc gacaatcctt gcaacaggtg
    37561 gtaatgccat ggttaaggcg gcttattcat gtggtaaacc agctcttggg gtaggtgccg
    37621 gaaacgttcc agcttatgtt gaaaaatcag caaacattcg tcaagcagca cacgatatcg
    37681 tcatgtctaa atcatttgat aacggtatgg tctgtgcatc tgaacaagca gttatcattg
    37741 ataaagaaat ttacgatgaa tttgtagcag agttcaaatc ttaccacact tactttgtaa
    37801 acaaaaaaga aaaagctctt cttgaagagt tctgcttcgg tgtcaaagca aacagcaaaa
    37861 actgtgctgg tgcaaaattg aacgctgaca tcgttggtaa accagcaact tggattgcag
    37921 aacaagcagg atttacagtt ccagaaggaa caaacattct tgctgcagaa tgtaaagaag
    37981 ttggcgaaaa tgagccattg actcgtgaaa aattgtcacc agttattgca gttttgaaat
    38041 ctgaaagccg tgaagatggt attactaaga ctcgtcaaat ggttgaattt aacggtcttg
    38101 gacactcagc agctatccac acagctgacg aagaattgac taaagaattt ggtaaagctg
    38161 ttaaagctat tcgtgttatc tgtaactcac cttctacttt tggtggtatc ggggacgttt
    38221 acaatgcctt cttgccatca ttgacacttg gatgtggttc ttacggacgc aactcagttg
    38281 gggataacgt tagtgccatt aacctcttga atatcaaaaa agtcggaaga cggagaaata
    38341 acatgcaatg gatgaaactt ccttcaaaaa catactttga acgtgattca attcaatacc
    38401 ttcaaaaatg tcgtgacgtt gaacgtgtca tgatcgttac tgaccatgcc atggtagagc
    38461 ttggtttcct tgatcgtatc atcgaacaac tggaccttcg tcgcaataag gttgtttacc
    38521 aaatctttgc ggatgtagaa ccggatccag atatcacaac tgtaaaccgt ggtactgaga
    38581 ttatgcgtgc cttcaaacca gataccatca tcgcactcgg tggtgggtct ccaatggatg
    38641 ctgccaaagt aatgtggctt ttctacgagc aaccagaagt ggacttccgt gaccttgtcc
    38701 aaaaattcat ggatatccgt aaacgtgcct tcaagttccc attgcttggt aagaagacta
    38761 aattcatcgc gattccaact acatctggta caggatctga agtaacacca tttgccgtta
    38821 tctctgataa agcaaacaac cgtaaatacc caattgctga ctactcattg acaccaactg
    38881 tggcaatcgt agaccctgct ttggtattga cagttccagg atttgttgct gctgatactg
    38941 gtatggacgt attgactcac gcaacagaag catatgtatc acaaatggct agtgactaca
    39001 ctgatggttt agcacttcaa gccattaaat tggtctttga aaatctcgaa agctcagtta
    39061 agaatgcaga cttccactca cgtgagaaaa tgcataacgc ttcaacaatc gctggtatgg
    39121 cctttgccaa tgccttctta ggtatttctc actcaatggc ccataagatt ggtgcgcaat
    39181 tccacacaat ccacggtcgt acaaatgcta tcttgcttcc gtacgttatc cgttacaatg
    39241 gtacacgtcc agctaagaca gcaacatggc ctaagtacaa ctattaccgt gcagatgaaa
    39301 aataccaaga tatcgcacgc atacttggac ttccagcttc tactccagaa gaaggggttg
    39361 aatcttacgc aaaagctgtc tacgaactcg gtgaacgtat tgggatccaa atgaatttta
    39421 gagaccaagg aattgacgaa aaagaatgga aagaacattc tcgtgaatta gccttcctgg
    39481 cttatgaaga ccaatgttca ccagctaacc cacgtcttcc aatggtagac catatgcaag
    39541 aaatcatcga agatgcatac tatggctaca aagaaagacc aggacgccgt aaataattgt
    39601 ttatcagtct agaagcaaga caaaaactca atttgaggga aagatccagt aatttttcta
    39661 tgataaaagg catcctatca aggtttttga acacctgata ggatgccttt ttatgatatt
    39721 gaggcctttt tgcccttttt gaaaaactag aatagaaaca aaatatataa tagattgaaa
    39781 ctagaatagt acacatctgc ttctaaaa
//
LOCUS       DBERDK010000013        34621 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.013, whole genome shotgun sequence.
ACCESSION   DBERDK010000013 DBERDK010000000
VERSION     DBERDK010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 34621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34621
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.013"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(245..1879)
                     /locus_tag="ACL73L_000630"
     CDS             complement(245..1879)
                     /locus_tag="ACL73L_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771650.1"
                     /translation="MRLKVIKKLVDINILYSSQEANLANLRKKQAKNTGKKVNVSARV
                     LSSYIFSSLLMLFMFINIAFRFPFEEMPSFFSTMVAILLVLAFSTSFTAFYNVFYESK
                     DLASYRPYAFKESEIIMAKGLSVLLPALAGIVPILAYFLVLYIRLAPSLWLGLPLMLL
                     SLTLLFVSVTLVMVVAVHFLAQTRVFRKYQSIFSNVMIGIGVLIPLIFIFFLQSTFGS
                     IVDKVRDIPFLLYPLHIFYKIAVEPFSTEALVGLLAWIGLTLFLLYLTKKKVLPRFYD
                     VILLNSEDKVKKERRSKERISTTKKGFFRMVLRYHLTLLGQGTGVVTVLFTSAFFPYL
                     MMIGLISKIRDSQIVPDIHPPYWLPLFFIALFIAVVNNNITSLHSIALSLERENVDFL
                     KSLPFDFARYVKVKFWIIYAVQSFLPVLTLLGLSLYLGLPIISMIYLIVAWILASVIL
                     SCHHYFKDVKNLSTNWSSITDLVNRSNGIVAIVLLFIYSAILMALVIGSIFLVQSLST
                     ILAISLGVGALILLLALAIFGYHYYLSRILAEIEKR"
     gene            complement(1860..2609)
                     /locus_tag="ACL73L_000631"
     CDS             complement(1860..2609)
                     /locus_tag="ACL73L_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0771651.1"
                     /translation="MIEFQNVSKLYGDKEALSNLNLQIENGEIMGLIGHNGAGKSTTI
                     KSLVSIISPSSGRILVDGQELSENRLAIKRKIGYVADSPDLFLRLTANEFWELIASSY
                     DLTSSDLETSLARLLNVFDFAENRYQVIETFSHGMRQKVFVIGALLSDPDIWVLDEPL
                     TGLDPQAAFDLKQMMKEHAQKGKTVLFSTHVLEVAEQVCDRIAILKKGHLIYCGSVED
                     LRKDYPDQSLESIYLSLAGRKEEVSDASQGH"
     gene            2788..4242
                     /locus_tag="ACL73L_000632"
     CDS             2788..4242
                     /locus_tag="ACL73L_000632"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HGS0771652.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKAQGIQPMADVVL
                     NHKAAADHREAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNDFHWHWYHFT
                     GTDYDAKRRKSGIYLIQGDNKGWANEGLVDNENGNYDYLMYADLDFKHPEVIQNIYDW
                     ADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDY
                     LEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKPDKAVTFVDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQEDFKEILDRLLAIR
                     KDLAYGEQNDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVD
                     LLGNHQGQVTIDEEGYGQFPVSARSVSVWAVNTI"
     gene            complement(4331..6949)
                     /gene="alaS"
                     /locus_tag="ACL73L_000633"
     CDS             complement(4331..6949)
                     /gene="alaS"
                     /locus_tag="ACL73L_000633"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HGS0771653.1"
                     /translation="MKQLSSAQVRQMWLDFWATKGHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPRITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLTSPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDPSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPHKNIDTGAGLERLVAVIQGAKTNFETDLFMPIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTV
                     GKIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVADLKEKGQSVIAGQ
                     DVFKLYDTYGFPVELTEEIAEEAGMTVDCEGFEAAMKEQQERARASAVKGGSMGMQNE
                     TLQNITVESVFNYNASQLSSKLVAIVADNAEVGAVSEGTASLIFAETSFYAEMGGQVA
                     DYGQILDESGKVVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLAIDSNRRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVDNTSEIGL
                     FKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAIAATLKAPQVKEVPHKVEGLQ
                     EQLRQLQKENAELKEKAAAAAAGDIFKDVKEVNGHRYIASQVSVSDAGALRTFADNWK
                     QKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELAPIIDGRGGGKPDMAMAGGS
                     NQAKIQELLDAVAGKL"
     gene            complement(6971..7456)
                     /locus_tag="ACL73L_000634"
     CDS             complement(6971..7456)
                     /locus_tag="ACL73L_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HGS0771654.1"
                     /translation="MKTFLVKQKFRLGGERFAIKDDRGEIAYQVEGSFFKIPKTFTIY
                     DAAGEQVSQISKEILTLLPRFEIQLRDGSSFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAEIKKELFHLTSTYTVTVLEDAYADLVISLCVAIDYVEMLESQS
                     H"
     gene            complement(7731..7970)
                     /locus_tag="ACL73L_000635"
     CDS             complement(7731..7970)
                     /locus_tag="ACL73L_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771655.1"
                     /translation="MKMSTFFNKSFWPTFMIVNQTAILFHLKDGLDRQYLTTESIYWV
                     IGTFIFGNILVAVFSNMKIWDKKKNGSKKKYILKK"
     gene            complement(8223..9293)
                     /locus_tag="ACL73L_000636"
     CDS             complement(8223..9293)
                     /locus_tag="ACL73L_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGS0771656.1"
                     /translation="MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLTQFTEETGIQVQYETFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKIEGIENIGPEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
                     WKPEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDLQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNVAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQNS
                     AYAFINFMLKPENALQNAEYVGYSTPNLPAKELLPEETKEDKAFYPDVETMKHLEVYE
                     KFDHKWTGKYSDLFLQFKMYRK"
     gene            complement(9290..10063)
                     /locus_tag="ACL73L_000637"
     CDS             complement(9290..10063)
                     /locus_tag="ACL73L_000637"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS0771657.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGDDMNSFTGFSWTH
                     FETMFGDGRLMLILAQTFFLAFLSALIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTQLKFSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLTPSIITGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            complement(10060..10866)
                     /locus_tag="ACL73L_000638"
     CDS             complement(10060..10866)
                     /locus_tag="ACL73L_000638"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS0771658.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVTLLISYPTALFLTRLKHRQLWIMLIILPT
                     WINLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDEMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGNRVITLGTAIEQNFLTNDNYGMGSTIGVILILTMFITMWVTKERRER"
     gene            complement(10847..12004)
                     /locus_tag="ACL73L_000639"
     CDS             complement(10847..12004)
                     /locus_tag="ACL73L_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0771659.1"
                     /translation="MKKPIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDIMLDGVRINDIPTNKRDVHTVFQSYALFPHMNVFENVAF
                     PLRLRKIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGTMIEDYLVEFNGKRFEAVDGGMKPNEPVEV
                     VIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIVGEEIG
                     LDFESEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEERDEENKL"
     gene            complement(12164..13069)
                     /gene="murB"
                     /locus_tag="ACL73L_000640"
     CDS             complement(12164..13069)
                     /gene="murB"
                     /locus_tag="ACL73L_000640"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HGS0771660.1"
                     /translation="MSVREKMLEILEGIDIRFKEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARLVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSGITLEREVRILGESK"
     gene            complement(13181..13933)
                     /locus_tag="ACL73L_000641"
     CDS             complement(13181..13933)
                     /locus_tag="ACL73L_000641"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family glutamic-type intramembrane
                     protease"
                     /protein_id="HGS0771661.1"
                     /translation="MTIHIIITMLLLLAFLIGSIWFAKKKYQINLAVLGLGAVAFFVS
                     SQILEKLVHILILHPQKDASIALLQDHPLIYIIYGLAMAAFFEETARLVFFKWLEKKR
                     SLEKADALAYGLGHGGLELIFLGLISLLNLYIVLSAVQTQNPQVMQLLSENMLKTIQS
                     LSVWQIYLLGFERILALGFQLLLTVWVYQAVRQKKWIYLLAAYGLHAFFDLAPSLFQV
                     GWLTNPVLVEVILALELVLVAYGTKEIFCKKS"
     gene            complement(13943..14674)
                     /gene="budA"
                     /locus_tag="ACL73L_000642"
     CDS             complement(13943..14674)
                     /gene="budA"
                     /locus_tag="ACL73L_000642"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HGS0771662.1"
                     /translation="MDKNVQEPVKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKGSGDQPEIVEVSPDALIPYAAVVPHQAEVIFRQRFEMTD
                     KELKERIESYYDGENLFRSIKIRGEFLHMHVRMIPKSTPDTKFADVATHQPEYSRDNV
                     AGTIVGFWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            14816..16087
                     /locus_tag="ACL73L_000643"
     CDS             14816..16087
                     /locus_tag="ACL73L_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HGS0771663.1"
                     /translation="MKKQSKYKEVVSYLKNGIESGRFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFEQALYCKANQLVLTSGTQQALFILSQISFP
                     RQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLEELEGHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKRSILNLAAKYDVYIVEDDYLGDLDSKKGQTFHYLDTEERVIYI
                     KSFSTSLFPALRITALILPNAIKEAFVAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LARLTNHESYQKQIEERITKTPCLLPHYPLHDGLLLDLRQYPKIASLKHSQPGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            complement(16107..16922)
                     /locus_tag="ACL73L_000644"
     CDS             complement(16107..16922)
                     /locus_tag="ACL73L_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGS0771664.1"
                     /translation="MKSKKWIFVLCNFLASFFLVACQSGSNGSQSAVDAIKQKGKLVV
                     ATSPDYAPFEFQSLVDGKNQVVGADIDMAQAIADELGVKLEISSMSFDNVLTSLQTGK
                     ADLAVAGISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGT
                     VPESMVKEQLPKVQLTSLTNMGEAVNELQTGKIDAVHMDEPVALSYAAKNAGLAVATV
                     SLKMKDGDANAVALRKNSDDLKEVVDKVIQKLKDEGTYQSYLEKAASLTEVEE"
     gene            complement(17079..17732)
                     /gene="phoU"
                     /locus_tag="ACL73L_000645"
     CDS             complement(17079..17732)
                     /gene="phoU"
                     /locus_tag="ACL73L_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HGS0771665.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRMKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
     gene            complement(17744..18502)
                     /gene="pstB"
                     /locus_tag="ACL73L_000646"
     CDS             complement(17744..18502)
                     /gene="pstB"
                     /locus_tag="ACL73L_000646"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HGS0771666.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEITALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLDPQHKETEDYITGKFG"
     gene            complement(18515..19318)
                     /gene="pstB"
                     /locus_tag="ACL73L_000647"
     CDS             complement(18515..19318)
                     /gene="pstB"
                     /locus_tag="ACL73L_000647"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HGS0771667.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLRQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            complement(19329..20213)
                     /gene="pstA"
                     /locus_tag="ACL73L_000648"
     CDS             complement(19329..20213)
                     /gene="pstA"
                     /locus_tag="ACL73L_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HGS0771668.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVVHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIF
                     NFGARKFGSYLHKKLTAA"
     gene            complement(20203..21120)
                     /gene="pstC"
                     /locus_tag="ACL73L_000649"
     CDS             complement(20203..21120)
                     /gene="pstC"
                     /locus_tag="ACL73L_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HGS0771669.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGTWNPSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFMTEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVNNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            complement(21120..21998)
                     /gene="pstS"
                     /locus_tag="ACL73L_000650"
     CDS             complement(21120..21998)
                     /gene="pstS"
                     /locus_tag="ACL73L_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HGS0771670.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMK
                     VEKDAAGTVTVLEGRQ"
     gene            complement(22572..23876)
                     /locus_tag="ACL73L_000651"
     CDS             complement(22572..23876)
                     /locus_tag="ACL73L_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HGS0771671.1"
                     /translation="MKFPEGFVEKYKEILGDEARDFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
                     YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGRLVY
                     STCTWAPEENEEIVNWLLEEYDFDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFV
                     AHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQIFGDQLYLLPEL
                     LPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKPSQVEQSVEIGQEAFVKYAAGET
                     VQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
     gene            complement(23877..24650)
                     /locus_tag="ACL73L_000652"
     CDS             complement(23877..24650)
                     /locus_tag="ACL73L_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HGS0771672.1"
                     /translation="MESKFEFAKELVKKAGQYILDHMQEDLRVETKSSPTDLVTRLDK
                     EVQELLVGEILSRYPEDKICAEEGCLRASVQEGKVWVIDPIDGTNNFVAQQEDFAVMM
                     AYFENGQGQFGLIYDVVKGDCYHGGGEFPVCLNDRPLAPFKTKPLGDFLIAGNSGMLE
                     TNEWGLADLSRSVLGVRVYGSAAISFAKILSGRLLTYLTYLQPWDYAAASILGESLGY
                     RVVTLFGEAPDFQTRQPVMMVPLEMQEEIQSYIYERKRT"
     gene            complement(24640..24918)
                     /locus_tag="ACL73L_000653"
     CDS             complement(24640..24918)
                     /locus_tag="ACL73L_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HGS0771673.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKGEGKISLGK"
     gene            complement(24918..25319)
                     /locus_tag="ACL73L_000654"
     CDS             complement(24918..25319)
                     /locus_tag="ACL73L_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HGS0771674.1"
                     /translation="MITLFLSPSCTSCRKAKAWLEKHKVPFVEHNIMTSPLTRKELQH
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
     gene            complement(25529..26146)
                     /locus_tag="ACL73L_000655"
     CDS             complement(25529..26146)
                     /locus_tag="ACL73L_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HGS0771675.1"
                     /translation="MKTKLIFWGSMLFLLSLSILLTIYLAWIFYPMEIQWLNLTNRVY
                     LKPETIQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLVQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLALVVLPVMIGLGGVLIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYENFFGYLCLKSRRK"
     gene            complement(26136..26909)
                     /locus_tag="ACL73L_000656"
     CDS             complement(26136..26909)
                     /locus_tag="ACL73L_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HGS0771676.1"
                     /translation="MKYKGYLIDLDGTIYKGKDRIPAGETFVHELQKRDIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTVYTATLATIDYMNDLGLEKTVYVVGEAGLKEAIK
                     AAGYVEDKEKPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     AGSLITLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIRAGIDN
                     GIPTLLVTTGFTKAEEVAGLPIAPTHVVSSLAEWDFDEN"
     gene            complement(26920..27657)
                     /locus_tag="ACL73L_000657"
     CDS             complement(26920..27657)
                     /locus_tag="ACL73L_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HGS0771677.1"
                     /translation="MGLTYQMKMKIPFDMADMNGHIKLPDVILLSLQVSGMQSIELGV
                     SDKAILEEYNLVWIIVEYDIEVVRLPRFAEEITIETEALSYNRLFCYRRFTIYNEAEQ
                     ELIHMMATFVLMDRDSRKVHVVEPEIVAPYQSDFDKKLIRGPKYESLEEPISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSAVERTG
                     LESKHEITSDGVINAQAIITWQEIKKA"
     gene            complement(27662..28792)
                     /gene="hemW"
                     /locus_tag="ACL73L_000658"
     CDS             complement(27662..28792)
                     /gene="hemW"
                     /locus_tag="ACL73L_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HGS0771678.1"
                     /translation="MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDAD
                     KIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            complement(28876..29265)
                     /locus_tag="ACL73L_000659"
     CDS             complement(28876..29265)
                     /locus_tag="ACL73L_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HGS0771679.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQVCSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQVESGEITVDSILAQAKSYAFQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIDITEE"
     gene            complement(29238..29664)
                     /locus_tag="ACL73L_000660"
                     /pseudo
     CDS             complement(29238..29664)
                     /locus_tag="ACL73L_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895042.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
     gene            complement(29665..30453)
                     /gene="lgt"
                     /locus_tag="ACL73L_000661"
     CDS             complement(29665..30453)
                     /gene="lgt"
                     /locus_tag="ACL73L_000661"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HGS0771680.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGALVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGATVDNLDYLPGFIRDQMY
                     IEGSYRQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFGFRVSQWLSVVLIGLGIMIVIYQNRKKAPYYITEEEN"
     gene            complement(30446..31381)
                     /gene="hprK"
                     /locus_tag="ACL73L_000662"
     CDS             complement(30446..31381)
                     /gene="hprK"
                     /locus_tag="ACL73L_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HGS0771681.1"
                     /translation="MSVLVKEVIEKLRLDIVYGEPELLEKEINTADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLPETPAVIVARGLVVPEEMLKAA
                     RECKIAILTSRAATSRLSGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            complement(31511..31687)
                     /gene="rpsU"
                     /locus_tag="ACL73L_000663"
     CDS             complement(31511..31687)
                     /gene="rpsU"
                     /locus_tag="ACL73L_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HGS0771682.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            complement(31767..32276)
                     /locus_tag="ACL73L_000664"
     CDS             complement(31767..32276)
                     /locus_tag="ACL73L_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771683.1"
                     /translation="MNKEEFLKIKEAYKSARTEERKSIIGFITKKKDKEGNFLFTKSK
                     DKPYTTRNQYSGGGGNKKYTSGSRLSRPYDLSNHMWIDLSYKGNDILISLQSFDIDPN
                     SKELHVLYDRIGILFEQSKKIPIFKDCYTITKVSDAFLKMETTNWELPLSEADMEEMV
                     NYIINHYEE"
     gene            complement(32354..33061)
                     /locus_tag="ACL73L_000665"
     CDS             complement(32354..33061)
                     /locus_tag="ACL73L_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HGS0771684.1"
                     /translation="MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYK
                     EIVESDLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHVVELDQSTIEANARF
                     FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLLEK"
     gene            33212..34240
                     /gene="queA"
                     /locus_tag="ACL73L_000666"
     CDS             33212..34240
                     /gene="queA"
                     /locus_tag="ACL73L_000666"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HGS0771685.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFH
                     SIIDMLEPGDALVMNDTRVLPARLYGQKVETGGHVELLLLKNTSGDEWEVLAKPAKRL
                     KVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDD
                     RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSEEAAATLRSVKENGGRVIAVGTTSIRTLETIGSKFDGQIQADSG
                     WTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHAIQEHYRFFS
                     FGDAMFIY"
BASE COUNT        10372 a         7879 c         6576 g         9794 t
ORIGIN      
        1 ttctaaatat atgttctatc aagaatactt ggaacacaca cattatagga attaacgttt
       61 ttgaaattga aaaatatcca aataaataaa ctataaacaa caaaaataga actatgttat
      121 atttcttatt caaaacattc ctccctatat ttttgattac caatcttaat catttacaac
      181 tacattctaa caaactataa aagcgtttgt cgaattgaat ttatcaagca agcgaccaac
      241 cagctcatct tttttctatt tctgccaata tgcgtgacag gtaataatga tagccaaaaa
      301 tagcaagagc aagcaggagg ataagagctc ctactcccaa gctgatggca aggatagtgg
      361 agagagactg aaccaagaat atgctcccaa ttacaagggc catcaggatt gcactataaa
      421 taaacaataa aactatggcg actatgccat ttgaacgatt caccaggtcc gtaatgctac
      481 tccaattggt tgacagattt ttaacgtcct taaagtaatg gtggcaagaa aggatgacac
      541 tggcaaggat ccatgccaca ataaggtaaa tcatcgaaat gatgggcaag cctagatata
      601 gagaaagacc aagcaaagtc agaactggta aaaaggactg gacagcatat ataatccaaa
      661 atttcacttt cacataacga gcaaagtcaa agggtaaact cttaagaaaa tcaacatttt
      721 ccctctccaa ggacaaggca attgaatgca ggctggtgat attgttattg acaactgcta
      781 taaagagagc tataaaaaac aagggtaacc agtatggagg atgaatgtct ggaactatct
      841 gagaatctcg gattttggaa atcagaccga tcatcatgag ataaggaaag aaagcacttg
      901 taaaaagcac tgtaaccacg ccagtaccct gtcccaagag ggtgaggtgg tagcgtaaaa
      961 ccatgcgaaa aaatcccttt ttagtggttg aaattctctc cttgctgcga cgttcttttt
     1021 tgaccttatc ctcactatta agcaggatca cgtcataaaa acgaggaagg accttctttt
     1081 tggtcagata aagcaggaag agagttagtc ctatccaagc gagcagaccc actaaggctt
     1141 ctgtcgaaaa aggctccact gctattttgt aaaagatatg aagaggataa aggagaaatg
     1201 gaatgtctct aactttgtca acaatacttc caaaagtcga ctgaagaaag aagataaata
     1261 ttaaaggtat gagaactcct atcccaatca tcacattcga aaaaatagac tgatactttc
     1321 tgaagaccct agtctgagcc aagaaatgta ctgccactac catcactaaa gtaacagaga
     1381 caaataataa ggtcaaggac agtagcatca aaggcaaacc caaccacaga gaaggtgcta
     1441 gcctaatgta taggaccaga aaataagcta ggattggtac aattccagct agagctggca
     1501 agaggacaga cagacctttg gccattataa tctctgattc tttaaaggca tagggcctat
     1561 aggaggccag gtccttactc tcataaaaga cattgtaaaa tgcagtgaaa gaagttgaaa
     1621 aggccagcac cagtaaaata gcaaccatcg tactaaaaaa acttggcatt tcctcaaaag
     1681 gaaaacgaaa ggctatattt ataaacatga aaagcatcaa gagactggaa aaaatgtaag
     1741 aacttaggac tctagcggaa acatttactt tttttccagt atttttagct tgcttctttc
     1801 gtaggttagc cagattagct tcttgagatg aatagaggat attgatatca actaattttt
     1861 taatgacctt gagacgcatc tgaaacctcc tcttttctac cagcaagact aaggtagata
     1921 ctttccaaag actggtctgg gtaatctttt ctcaagtcct ctacactacc acaataaatc
     1981 aaatgcccct ttttcaaaat ggcaatccga tcacagactt gctctgccac ctctaggaca
     2041 tgagttgaaa acaagactgt cttccctttt tgtgcatgtt ccttcatcat ctgtttcaaa
     2101 tcaaaggcag cctggggatc caaaccagtc aagggttcgt ccaagaccca aatatcagga
     2161 tcagacaaga gtgccccgat gacaaaaact ttctgacgca ttccatgaga aaaggtttca
     2221 ataacctgat aacgattttc agcaaaatca aaaacgttca atagcctagc tagactagtc
     2281 tccaagtcag agctagtcag atcataggat gaggcgatca attcccaaaa ttcattggcc
     2341 gttaaacgta aaaataagtc aggcgagtct gctacgtagc caatctttcg tttgatcgcc
     2401 aagcgatttt ccgataactc ctgaccatct accaaaatac gaccactgct gggtgaaatg
     2461 atactgacta gggattttat ggtggtcgat tttccagccc cattatgacc aatcaagccc
     2521 ataatctctc cattttcaat ctgcaaattg agattactca aggcctcttt atcaccatac
     2581 aacttactaa cattttgaaa ttcaatcatg ggatgactcc tatctttatc tgtattatac
     2641 atcgctatac tagcactttg atacaaaaaa atcaatactt tattttaagt agttaaaata
     2701 gtttccttct atcttacgca aacgtttgct ccgagagata ctttatgata gaataaagaa
     2761 caagattgac aagtaagagg aaacatcatg caaaatcaaa cactcatgca atactttgaa
     2821 tggtatctgc cccacgacgg tcaacactgg acgcgtctgg ctgaaaatgc tccacaccta
     2881 gctcatctgg ggatcagtca cgtctggatg ccaccagcct tcaaggcaac caacgaaaaa
     2941 gatgtcggct atggggtcta tgacttattt gacttaggag agttcaacca aaaagggact
     3001 gtccgcacca agtatggttt caaagaagac tatcttcaag ccattcaagc ccttaaagcg
     3061 cagggaattc aacctatggc cgatgtagtt ctcaaccaca aggctgctgc tgatcacagg
     3121 gaagcctttc aggttattga agtggatcct gtagaccgta cagttgaact tggagaaccc
     3181 ttcaccatca atggctggac tagttttacc ttcgatggtc gccaagatac ctacaatgac
     3241 ttccactggc actggtacca cttcacaggt acagactatg atgccaaacg ccgtaaatcc
     3301 gggatttacc taatccaagg agacaacaag ggctgggcta acgagggatt ggtcgataac
     3361 gaaaacggaa actacgacta cctcatgtat gccgacctag actttaaaca tcctgaagtc
     3421 atccaaaaca tctatgactg ggctgattgg ttcatggaaa cgactggtgt agctggtttc
     3481 cgtttggatg ccgttaagca tattgactct ttctttatgc gcaatttcat ccgcgatatg
     3541 aaggaaaaat acggtgacga tttctatgtt tttggtgaat tttggaaccc agacaaggaa
     3601 gccaatctgg actatctcga aaaaacggaa gaacactttg accttgtcga tgttcgtctc
     3661 caccagaatc tctttgaagc cagtcaagct ggcgcaaact atgaccttcg tggcattttc
     3721 acagatagcc tggttgaact caagcctgac aaggctgtga cttttgtcga caaccacgat
     3781 acccaacgag gacaagccct tgagtctacc gttgaagaat ggttcaagcc agcagcctat
     3841 gccctcattt tgttacgcca agacggcctt ccatgtgtct tttacggaga ctactatggg
     3901 atttcaggcc agtatgctca agaagatttc aaagaaatcc ttgaccgcct cctagccatc
     3961 cgaaaagatt tggcctatgg agaacaaaat gactactttg accatgctaa ctgtatcggt
     4021 tgggtacgtt caggtgctga aaatcaatcc ccaatcgcag tcttgatttc aaatgaccaa
     4081 gaaaacagca agtcaatgtt tgtcggtcaa gaatggacta atcaaacctt tgtagattta
     4141 cttggtaacc accaaggtca agttacaatt gatgaggaag gttatggaca attccctgtc
     4201 tcagctagat ccgtaagtgt ctgggcagtc aataccatct aatagctcat aataaccaag
     4261 ctaggtccaa gcggatttgg cttttttgta gtcaaaaaag acctacccaa atggatagat
     4321 ctttacttga ttacaattta cctgctactg catccaataa ttcttggatc ttagcttggt
     4381 tgcttcctcc tgccatggcc atgtctggtt taccaccacc acgtccatcg atgattggtg
     4441 ctaattcttt gacaaggttt cctgcatgaa ggtcttttgt cttgcttgct acaaggacat
     4501 tgactttgtc accgatagcg gcaactagga caagaagatc agagtagtct ttttgtttcc
     4561 agttatctgc aaaagtacga agggcaccgg catcggatac agacacttga ctagcaatgt
     4621 aacgatgacc gttgacttcc ttaacatctt tgaagatatc gcctgcggct gcagctgcgg
     4681 ctttttcttt caactcagca ttttcttttt gaagttgacg aagttgttct tgaagtcctt
     4741 ctaccttgtg aggtacttcc ttgacttgag gtgctttcaa ggttgctgcg atagctttaa
     4801 gagcatcctc ttgttcacga taggcttcaa aggcttcctt accagtcact gccaagatac
     4861 ggcgagttcc tgaaccgatt ccttcttctt tgacaatttt gaagagacca atctcagaag
     4921 tgttgtcaac atgagtacca ccacaaagtt caatagagta gtcaccgata gtcacgacac
     4981 gaacttcctt gccgtatttc tcaccaaaga gggccatagc tcccatttct ttagcagtgt
     5041 caatatccgt ttcaactgtc ttcacttcaa gtgcttccca aattttctcg ttaacttgct
     5101 gttcaatcgc acgaagttcc tcagcagtta ctgcttggaa gtgggtaaag tcaaagcgaa
     5161 ggaattcaac ttcgttaaga gatcctgcct gtgttgcgtg gtttccaagg atattgtgaa
     5221 gggcagcgtg aagcaaatga gtcgcagtgt ggtttttcat gacacggtga cggcgattgc
     5281 tatcaattgc caaggtatat tcttggttca aggcaagcgg tgcaaggact tcaactgtat
     5341 gaagggcttg accatttggg gctttctgaa cattggtcac agtagccaca accttacctg
     5401 actcatccaa gatttgtccg tagtcagcta cctgtccacc catttcagca taaaatgacg
     5461 tttccgcaaa gataagagag gcagttcctt ctgaaacagc tcctacttct gcattgtcag
     5521 caacgatagc taccaattta gaagacaatt ggctagcatt gtagttgaag acactttcta
     5581 cagtgatgtt ttgaagagtt tcattttgca tacccattga gccacccttg acagctgacg
     5641 cacgcgcgcg ttcttgctgt tctttcatgg ctgcttcaaa accttcacag tctacagtca
     5701 taccagcttc ttcagcaatt tcttcagtca attcaactgg gaatccgtat gtatcataga
     5761 gtttaaagac atcttgacca gcaatgacag attgaccttt ttctttcaag tctgccacaa
     5821 tgccttgggc aaagtgttga cctgagtgaa gggtacgggc aaatgattct tcttcgctct
     5881 taacgatttt ctcaataaag tcacgtttct caagcacttc tgggtagtag ctttccatga
     5941 tttttccaac agttggaaca agtttgtaaa ggaaaggctc gttgataccc aatttttgac
     6001 catgcataga agcacgacgg agaagacgac gaagaacata accacgacct tcatttcctg
     6061 gaagggcacc atcaccgata gcaaatgaaa gagaacgaat gtggtctgcg ataaccttga
     6121 agctcatgtt gtcgccatct tggtcataaa ccttaccaga caatttctcg acttcacgga
     6181 taatcggcat aaagaggtcc gtttcaaagt tggtcttagc cccttggata acagccacca
     6241 aacgctccaa accagcgccc gtatcaatgt tcttatgtgg caattccttg tattcgctac
     6301 gaggaacagc agggtctgcg ttaaattgtg acaaaacgat gttccagatt tcaatataac
     6361 ggtcgttttc aatatcttct gcaagcaggc gaagaccgat attttctggg tcaaaggctt
     6421 ccccacggtc aaagaagatt tctgtatctg gtccagaagg tcccgcaccg atttcccaga
     6481 agttgtcctc aattggaatc aagtgacttg gatccactcc cacttcaatc cagcggttgt
     6541 aagaatcttt atcgtctgga tagtaggtca tgtaaagttt ttcagcaggg aaatcaaacc
     6601 attcagggct tgtcaaaagc tcataagccc aagtgatagc ttcgtcacgg aagtaatccc
     6661 cgatagagaa gttccccaac atttcaaaca tggtatggtg acgcgcagtc ttccctacgt
     6721 tttcgatgtc gttggtacgg atagcctttt gggcattggt aatacgtgga ttttcaggga
     6781 taatggtccc gtcaaagtat ttcttaaggg ttgctacccc agagttgatc cacaaaagag
     6841 ttgggtcatt tacaggaacc aaacttactg atggttctac tgagtgacct ttggtcgccc
     6901 agaaatcaag ccacatttgg cgtacttgtg cactagatag ttgtttcata ttgtctcctt
     6961 attcacttgt ttaatgtgat tggctttcca gcatttccac atagtcaatc gcgacacaga
     7021 gggaaatgac taggtctgca taagcgtcct caagaaccgt tacggtatag gtagaggtca
     7081 gatggaagag ttccttctta atttccgcaa tcaactgatc gcgatcatcc agcaatttga
     7141 aattcaaatc ccagatattg ccctcgatac gaagacctag attatcaaac tcatacttat
     7201 ctcgccaaaa ggtcaacttc ttacgaatga caaaactcga gccatcccga agctgaatct
     7261 caaaacgagg aagcaaggtc aagatttctt tactgatctg actgacttgt tcaccagccg
     7321 catcatagat ggtaaaagtt ttgggaatct taaaaaatga tccctccacc tgataggcaa
     7381 tttctcccct gtcatccttg atagcgaagc gttcgcctcc aagacgaaac ttttgtttga
     7441 caagaaatgt tttcatcaac acctccaaaa atcaaaagac aagctcatat cacgaagggc
     7501 gaaaaaccgc ggtaccacct tcattcaatg aacttgtcat tctcttgttc ttatgcaatt
     7561 gtatgattga gtagcatgac ttcctagctt agatggctcg cagcaccgcc atttctctgg
     7621 actaagacaa gtgaaaatca attctcaact ttcttattat atcgtttttt taatcttgcg
     7681 tcaactggaa atgatctccg ttgaattaga ccaattccct acatctctga ttactttttc
     7741 aggatatatt ttttcttact gccgtttttc tttttatccc aaattttcat attactaaac
     7801 acagctacta gaatatttcc aaatataaag gtgcctatca cccaatatat ggactcagtt
     7861 gttaggtatt gtcgatccaa gccatccttt aaatggaata gtatagcagt ttggttaaca
     7921 atcataaagg ttggccagaa acttttgttg aaaaaagtag acattttcat tatttgttgc
     7981 cgctttctgt aaggttaata ctcaataaaa atcaaaaagc aaactaggaa gctagcctca
     8041 agctgtactt gagtacggca aggcaacgct gacgtggttt gaagagtata ggcttagtat
     8101 actactaggc aagcaaataa gcaaataaac aactagaata gaaaaagata gggctctaaa
     8161 aactgacttc tattccttaa aaacgaacca gcttgactga ttcgtcttct tacatttatc
     8221 tcctacttcc gatacatttt aaactgtagg aagaggtcgc tatatttccc tgtccattta
     8281 tggtcaaatt tctcataaac ttctaggtgt ttcatggttt caacatcggg atagaaggcc
     8341 ttatcttcct ttgtttcctc tgggagcaat tccttcgctg gtaggtttgg tgttgaatag
     8401 ccgacatact ccgcattttg gagagcattt tcaggtttca acataaagtt gataaaggca
     8461 taggctgagt tttggttttt aactgttttg ggaatgacca tattgtcaaa ccaaagattg
     8521 ctggcctctg tcggtaccac ataacgtaga ttttcatttt tttctaacat ttggctggct
     8581 tcaccagaga aggtcacgcc gattgcaaca ttattctgaa tcatatagcc cttcatctcg
     8641 tccgcaacga tagccttgat atttggagtc agtttgtaga gcttatccac tgtctcttcc
     8701 aactgctgca gatccttgga gttgaggctg tagccgaggg aattgagtcc tagtcccagc
     8761 acctcacgcg ccccatcaaa gagcatgata gaattcttat actccggctt ccaaaggtca
     8821 tcccaatgct caggcgcttc atctaccatg gtttcgttgt agacaattcc taaggttccc
     8881 cagaagtaag ggatggagaa tttattacct gggtcaaagg actggttgag aaactctggt
     8941 ccgatatttt cgattccttc aatttttgaa taatcaagcg gaaccaagag gtcttcgtcc
     9001 ttcatcttgt taatcatgta ttcacttgga atggcaatat cgtaggtcgt tccaccctgc
     9061 tttatcttag tgtacatggc ttcgttggag tcaaaagtct cgtactgaac ttgaattcct
     9121 gtttcttctg taaactgagt caagagttca ggatcgatat agtctcccca gttatagata
     9181 accaattttt gactatctcg actattgatt ttactatcta aatgagtcgc aattccccac
     9241 aagacaagga taatcgctgc aattcctgct aaaaatgaat agattttttt catgcttgct
     9301 cctccttctc acgagagata aagtaataac ctacaactag gataatacta aagagaaaga
     9361 ctagagcaga cagggcattg atttctaagg aaatcccctt gcgagcacga gagtaaatct
     9421 cgactgatag ggttgaaaag ccatttcctg ttacaaagaa ggtcacggca aagtcatcta
     9481 acgaataggt gaaggccatg aaataaccag taatgataga cggagtcagg taaggaagca
     9541 tgatttcctt gaacatctga aattgactag ctcccaagtc ataggccgca tgaatcatgt
     9601 cgccattcat ttccttgagt cgaggcaaga ccatcaagac cacgatagga atggagaagg
     9661 ccacgtgact agatagaacg gtcaaaaagc caagtgaaaa cttgagttgg gtaaagagaa
     9721 tcaagaagct agcaccaatc ataacgtcag gcgcaaccat gaggatatta ttgagtgata
     9781 gaaaggcttc ttggtatttc ttacgagact ggtagatgta aatggcacca aaagtcccga
     9841 taatggtcgc tatcaaggct gataggaagg ccaagaaaaa tgtctgagcc aaaatcagca
     9901 tgagtctccc atctccaaac atggtttcaa agtgagtcca gctaaaacct gtaaagctat
     9961 tcatatcatc accagcatta aaggcatagc caatcaagta aaaaatcggc aggtagagga
    10021 ccagaaagac cagtcccaga taaaggttgg caaatttttt catcgttctc tcctttcctt
    10081 agtcacccac atggtgatga acatggtcag gatgagaatc acaccgatag ttgaacccat
    10141 accatagttg tcattggtta gaaaattctg ctcaatagcc gtccccaagg tgataacgcg
    10201 gttcccacca atcaaacggg tcagcatgaa gagactcaaa cttgggataa agaccgactg
    10261 aaccccactt cgcacaccgt tcatagatag agggaagatg acatgacgga aggtctccca
    10321 cttagttgca ccaaggtcgt aactagcatt gatgagatta ttatccattt cgtctaagac
    10381 attgaaaatc ggcaaaatca taaaggggag ctcgatgtag cttgcgacaa agataaatga
    10441 gaaatcagta aatagcaact gctgtgaacc aattccgata aattccaaga attggttaat
    10501 agagccattc tgaccaaaaa tcccgataaa ggcataagcc ttaagaagaa ggttgatcca
    10561 agttggcaga ataatcagca taatccagag ttgacggtgt ttgagacggg tcaaaaagag
    10621 ggccgtcgga taactgataa gcagtgtcac aaaggtcaca atgcctgcat aaagcactga
    10681 gttgaaactc attttaagat aggtcaagtt ttgtgacgca aagtaagatt tgtaattttc
    10741 taaactgaac tggccttcga tgttgaaaaa ggattgaccg aaaatcaaga ccaagggtgc
    10801 caatacaaag agcgcaatcc aaagcatgta gggtactaca aagagtttag agcttgtttt
    10861 cttcatctct ttcctcctcg attgcattga tcaaacctgc ttcttgctct tcgatttcca
    10921 cgtattcttc gatacgagca tcgaactctt cttcggtttc attgagacgc atgatgtgga
    10981 tgtcttctga ttcaaagtcc agaccgattt cctcacccac gatggcctta cgggttgagt
    11041 ggatcatcca ttcatttcca agttcgtcat aggcgataat ttcataatga actccacgga
    11101 agagctgggt gtcgacctta acttggagct tgccttcttc aggaagggta atgcgcaagt
    11161 cctctggacg aataacgacc tcaacaggtt catttggctt catcccacca tcaaccgctt
    11221 caaagcgttt gccgttaaat tcgaccaagt agtcctcaat catggtacct ggcaagatgt
    11281 ttgactcccc gataaaggtg gcaacaaagt ggttgattgg ctcatcgtag atgtccacag
    11341 gggttccaga ctggacaatc tcgccatcat tcataacgaa aatccagtca ctcatggcaa
    11401 gagcttcttc ctgatcgtga gtgacaaaga caaaggtaat gcccaatcgt tgttgtaatt
    11461 cacgcaattc gtactgcatg tctgttctca atttcaagtc cagcgctgat aaaggctcgt
    11521 ccaacaagac cacacggggt tggttgatga tagcacgggc gatggccaca cgctgacgtt
    11581 gtcctccaga aagtttgcgg atggaacgtt tttcataacc ttccaactga accatcttga
    11641 gaacttccgc tacacgctgc tcgatttctt tcttatcaat tttacgcaag cgaagtggaa
    11701 aggcaacatt ttcaaacaca ttcatatgtg ggaacaaggc ataggattgg aagacggtat
    11761 gtacgtcgcg cttgttggtt ggaatatcat tgatacgaac accgtctagc atgatatctc
    11821 ctgtcgtcgc atccagtaaa cctgcaataa tgtttaggat agttgatttc cccgaaccag
    11881 atgcacctag aagggtgtag aatttccctt cttccaactc aaagttgatg tctttgagaa
    11941 ccttggtgtt gctgtcttca aaaactttag agacgttttt gaattcgata attggttttt
    12001 tcaattggca taaattcctt ctttttcata gattaaccga tcggggctct gtcaggtccc
    12061 cactacctct tgcagggagt aaaaccacct gcatacatct tcgctaccga taggctttca
    12121 tcccctttcc atgacatagc agcagctttt caaatacagc ttcctacttg ctttcaccca
    12181 agatccggac ttctctttca agcgtaatac ctgagtgttc cttgactttt tcgataaccg
    12241 attggatcaa gtcctcgtag tctttggccg ttccatctgc gacattgatc ataaatcctg
    12301 catgcttttc tgacacttct acgccaccga tacgatagcc tttcaagcca gcttctgaaa
    12361 ttaactgacc tgcaaaatgc ccgactggac gcttaaagac cgagccacaa gatgggtatt
    12421 ccaaaggttg cttgagttca cgtaggtgcg tcaagcggtc catttcctgc ttgataacct
    12481 gatgggttcc tggggctagg gcaaatttaa ctgacaagac aactgcacca gactcctgaa
    12541 tagctgaatg gcggtaacca aaagccaagt ctttagcaga cagggtttcg atttctccat
    12601 ccttggtcaa gaccttacaa gactgcaaga tgtgagcaat ctcgccacca taggcacccg
    12661 cattcataaa gacagcaccg ccaacgcttc ctggaatacc acaagcaaac tcaaagccag
    12721 tcaaactatg acggagggca atgcgagttg tttcaatcaa gttagcccca gcttctgctt
    12781 caatggtata gccatcaaca gaaacgttat tgagcttgtc acacaagatg acaaatccac
    12841 gaatcccacc atcacgaacg atgatattgc ttgcattgcc aagaaccatc caagggatat
    12901 tttcttggtt ggcaaatttc acaaggcgcg ccaactcaaa acgatttcgt ggaaagacca
    12961 aataatcagc ctctccacct acttttgtat aactatagct atgcaagggt tccttaaaac
    13021 ggatatcaat tccttctaag atttcaagca ttttttctct tacagacatg tcactcttcc
    13081 ttttacaaaa ttcattccat tataccattt ttagagacat ttgacgacca taaaaatacc
    13141 ttgtttggat tttgcataag aaaaagagtt tccccccttt ttatgatttt ttacaaaaga
    13201 tttccttggt tccataggcg accagaacga gctccagtgc tagaatcact tcaaccaaga
    13261 ctggatttgt caaccagcct acttggaaaa gagatggtgc cagatcaaag aaggcatgca
    13321 agccataggc tgctaggaga taaatccatt tcttctggcg aacagcttgg taaacccaaa
    13381 ctgtcaaaag taattggaaa ccaagcgcca agattcgctc aaaaccaagc aaataaatct
    13441 gccagaccga aagtgactga atggttttta acatattttc agacagtaat tgcataacct
    13501 gtggattctg agtttgaact gccgaaagaa cgatgtaaag attgagtaaa ctaataaggc
    13561 ctagaaaaat caactccaag ccaccatgcc ctaggccata ggccaaggca tctgcctttt
    13621 ccaaacttct ctttttctcc aaccatttga agaaaacaag acgagccgtt tcctcaaaaa
    13681 atgctgccat ggctaggcca tagataatat agataagcgg atgatcttgc aagagggcaa
    13741 tactagcgtc tttttgaggg tgcaaaatca agatatgcac cagtttttcc aaaatctgcg
    13801 aagagacaaa gaaagcgact gcacccaagc ccaagacagc tagattaatc tggtatttct
    13861 ttttggcaaa ccaaatgctt cctatcagaa aagctagcaa cagcaacatg gtaataatga
    13921 tatgaatggt cattttcttc tcctattctg ccttttcaat atcttttttc atctcgtcaa
    13981 cattgaactt agcaaacaag tattgacggt cttggactgg gaaacgttgg tccaactggt
    14041 caactgctcc cacctcgata atgccttcct tgatgacaaa atccatgaca tgtccaccga
    14101 aggtcaaatc atctgatatg aagtgcagat ggtagcctgc cacactgacc ccatggaaaa
    14161 tctcaggcgt ccagaaacca acaatagtcc ccgcaacatt gtcacgacta tattccggtt
    14221 gatgggttgc gacatcagca aacttggtat cgggtgttga cttaggaatc atacgcacat
    14281 gcatatgtaa aaattccccc cgaatcttga tagagcggaa aagattttcc ccatcataat
    14341 aagactcaat tcgttctttc aattccttgt ctgtcatctc aaagcgctgg cggaaaatga
    14401 cctctgcctg atgcggtacc actgcagcgt aaggaataag ggcatctggt gacacttcca
    14461 caatttctgg ctggtctcct gaccctttgg cctgataagc cttgccatcc aagacaatca
    14521 attccccatc aatggaatcc aaggttccca aaccaaggtc accatgttct agcaattctc
    14581 ctactgtcat agtaccacca taaaggccag ccatcaaggc tccaagagta ttgtattgaa
    14641 ataatttcac tggttcctgc acgttcttat ccattcactt tcttgtctgt tattctataa
    14701 atcttactcc taagtatacc acatttgccc ctagatgtga acgagagaaa cgctctagac
    14761 attgccaaga aggaaaaaaa agggtacaat gtaacaaaat caagggaggt ctggaatgaa
    14821 gaaacaaagc aagtacaaag aggtcgtttc ctatctgaaa aatggtatcg agtctggacg
    14881 atttccgacg ggtagtcgcc tgccttctat ccgtcaactg agccttgact ttcactgcag
    14941 caaggacacc attcaacgag ccctgctgga attacggcac gaacaatacc tctatgccaa
    15001 gcctcagagt ggctactatg tattagaaca agggcaacat caagacctag aaatcgaggt
    15061 taccgacgaa catgccagtg cctatgacga tttccgactc tgtgtcaatg aaaccttgat
    15121 tggccgagaa aactacctct tcaactacta tgacaatcaa gaaggattag aagacctaag
    15181 acagtccatt cacaaactcc tctttgagca agctctctac tgcaaggcta accaactagt
    15241 actgacttct ggaacccaac aagccttgtt tatcctctct caaatatcct ttcctagaca
    15301 agccaaggaa atcttggtgg aacagccaac ctaccatcga atgaatcgcc tcttgattgc
    15361 acaggggctg gactatcaaa cgattgaacg aggcattgat gggattgact tggaggagct
    15421 ggaaggccac ttcaaaacag gaaaaattaa gtttttctac accattccta gatttcacta
    15481 tcctctggga cattcctatt ctgagcagga caaacgatct attcttaact tagctgccaa
    15541 gtatgatgtc tatatcgtag aggacgatta tctgggtgat ttggactcca agaagggcca
    15601 aaccttccac tatcttgata cagaggagcg tgtcatttat atcaagtcct tctcgaccag
    15661 cctttttcct gcccttcgta ttacagcact cattcttcca aatgctatca aagaagcatt
    15721 tgtggcctac aaaaatatcc tagactacga cagcaacctc attatgcaaa aggccctgtc
    15781 actctatatt gacagtcaat tgtttgaaaa aaatcgtttg gctcgcttga ccaatcatga
    15841 atcttaccaa aaacaaatcg aggaaaggat aactaaaaca ccttgtctcc ttcctcatta
    15901 tcccctacac gatggtttat tgctagacct gagacagtat cctaaaatcg ccagtctcaa
    15961 acacagtcaa ccgggcttgg acttctttga agaggcctat ttaagtacct gtccttatca
    16021 atttgccaag gtgtccctag acaatctgga aaatgtttta aactatttaa aagcagaatt
    16081 ggaatgacat ccaactctgc tttttcttat tcttcaactt cagttagact tgctgctttt
    16141 tcaagatagc tttggtaagt tccttcatcc ttaagttttt ggatgacctt gtctaccact
    16201 tctttcaaat catcactatt ttttctaaga gcaacggcat tggcgtcgcc gtccttcatc
    16261 ttcaagctga cagttgcgac agctaagcca gcgtttttag cagcataact aagtgcaaca
    16321 ggctcatcca tatgaacagc atctattttt ccagtctgca attcattgac tgcttcaccc
    16381 atattagtta gggaagttaa ttgaactttt ggcaattgtt ccttgaccat tgattctgga
    16441 acagtccctt tttgggctgc aatattagca ctttctaggc tagttaaatc cttgtatttt
    16501 tccacatcag ccttacgaac caagaaacta atcttgtttt catagtatgg gattgaaaaa
    16561 tcaaagactt cttttctctc gtcagtagca ctaattcctg caactgctag gtcagcctta
    16621 ccagtttgaa gactggtcaa aacattgtca aaactcatgc ttgagatttc caacttaacc
    16681 ccaagttcat cagcgatagc ctgagccatg tcgatgtctg caccgactac ctggttcttt
    16741 ccatcaacca atgattgaaa ttcaaagggt gcatagtcag gactggttgc cacaactaat
    16801 ttcccttttt gtttgatagc atccacagca gactgagaac cattagaacc tgactgacaa
    16861 gctactaaga agaaacttgc aagaaaatta cataaaacaa atatccattt ttttgatttc
    16921 ataaataaac aacctctctg ttaattttgt ataattataa cgatatccaa gttacttgtc
    16981 aagtgttttt taaattttta tctcaaaaat attttttcgt tcaaaaaaag gagccatcag
    17041 ttgatttcaa gctccttttt atacagaatt aaactatttt atagttcgac aatcttacct
    17101 gtttcaaagt agacaaccca ttcacagata tttttagcat agtcaccgat acgctccaag
    17161 taggaaataa cttggaaata atcacgaccc gtaacaatgg cttctggatt tttcttaatc
    17221 tcttcagtcg caaggtcacg gatagtttca aaatagtggt taatttgctc atccatggag
    17281 gccacccggt atgcgtcgtc aacagaacca ttaagataaa gatcaagtgc tgcttcaaca
    17341 acgcttttaa cttcacgtcc cattttttta atttcttcct ctacagctgg aatgcgctct
    17401 tcccccttca tacggatggt tgcctgggca atggctacag cgtgatcccc catacgctcc
    17461 acatctgata cagccttaag gacagtcaag actgtacgca aatcttgaga gactggttgt
    17521 tggagtgcga tcatttcaaa tgatttcttt tccagtttca cttcgtattc atttacttct
    17581 gcatcatctt cgatgacctc ttttgccagg tcacggtcat gcgtgacaaa agcacgtacc
    17641 gtacgattga tttgtgagag cacttcttgt cccatagcgt agaactggtt atgtaatttc
    17701 tctaaatctt cttcaaattg agatcgtaac atctttcatc tccttatcca aattttcctg
    17761 taatatagtc ttccgtttcc ttgtgttggg gatcaaggaa catctgcttg gtatcattaa
    17821 attcaatcaa atctccatct aggaaaaatc ctgtcttatc agagatacgt gaagcttgct
    17881 gcatggaacg ggttaccaga agcatggtgt acttgtcttt tagaccatac aaggtttcct
    17941 caattttacc agctgaaatc ggatccaaag ccgaagttgg ctcatccaag aggatgattt
    18001 taggactagt tgccaagaca cgggccacgc agacacgctg ctgttgacca cctgacaatc
    18061 caatagctga atcatataga cgatccttga cctcatccca gatagaggca ccttgcaagg
    18121 ctttttctac ggcttcatcc agaacctgct tatccttaat tccattgata cgaagcccgt
    18181 agacaacatt ctcatagata gtcataggga aaggattagg ttgttggaaa accattccga
    18241 tttccttacg taattcaacc gtatctgtac gcggactgta gatgttgtga ccattgtaca
    18301 ccacggatcc agttgtggtc acctctggat tgagatctcc catgcggttg agagacttga
    18361 ggagggttga cttccctgat ccagatggac caatcaaggc tgtaatttcc ttaggttgga
    18421 aagataggga aacactattc aaagccttct ttttattata ataaacggac aggtctgata
    18481 cctgtaaaat cgcatctgtc atacggtttc ctttctaacc aaagtgacca gatacatagt
    18541 cattggtgga ctgtagcttg gcattttgga aaatagttgc agtcttgtca tactcaatca
    18601 aatcacccaa gtaaaagaag cctgtatagt cacttgcacg agcagcctgc tgcatattat
    18661 gcgttacaat gatgatggta aagtttttct tgagctcaaa catggtctct tctagttgca
    18721 tggtcgcaat cggatccaag gctgaggctg gctcatccat taagaggata tctggcttaa
    18781 cagagatggc acgagcgata cagagacgtt gttgctgacc acctgataag gtcaaggctg
    18841 acttgtggag atcgtcttta acctgatccc agagggcagc ctgacgaagg gaggtttcta
    18901 cgatttcatc taggacttgc ttatccttaa ctccagcacg ttcatgcgca aaggtaatat
    18961 tacggtaaat tgacttagca aatggattgg ggcgttgaaa aaccattcca atgtgtttac
    19021 gcatttcata aacgttgatt tctggacggt tgacatcaat tccacgatag agaatctgcc
    19081 cagttacttt agcaatatca atagtatcat tcatgcgatt gagactgcgt aagtaggtag
    19141 atttccccga tcccgacggg ccaatcaaag ctgtaatttt atttctttca aattgcatat
    19201 caatcccctt aatggattca tttttaccat agtaaacatg gacatcctta gtagaaaggg
    19261 ctactttttc ttcaggaaag gtaaggatat gcttctcatc ccagttatat gttgacatgg
    19321 cttctccttt aggcagcggt taatttcttg tgtagatagc ttccgaactt acgagctcca
    19381 aagttaaaaa tcaggataaa gatcaggagc acagcggcag aacctgctga tacaatggtt
    19441 ccatctggaa tagtgccttc actattgact ttccagatat ggacagccaa ggtttctgct
    19501 tgacggaaga tagagatggg gctagtcaca ctgaggatat tccagttaga ccagtcaaga
    19561 gctggcgccg attgccctgc tgtatagatc agagctgcag cttcgccaaa gatacgacca
    19621 gatgccaaga cgacacccgt tacaatacct ggaagcgctt ctggaataac aacatgaacc
    19681 actgtctccc agcgagaaat cccaagagcc agaccagcct cacgttgggt atggtgaacg
    19741 tgtttcaaac tatcctctac attacgcgtc atctgaggca agttaaagac tgtcaaggcc
    19801 aaggcacctg aaatgattga aaatccatac tcaaactgga ctacaaagat caagtaacca
    19861 aagagaccca ccaccactga tggtaaagag gacaagattt caatacaagt ccgcacaaag
    19921 ttggtaacag gacctttttt agcatattca gccaagtaaa tcccagctcc catagaaaga
    19981 ggtacagaaa taatcaaggt aatgaccaat aggaaaaagg aattgtaaag ctgaatgcca
    20041 atcccaccac ctgcttgaaa agcagaagac cttccagtca agaaagacca agagatatgg
    20101 ggcaagcccc gaaccaagat atagagaatc aaggaagcca agattgtcac aatgatgcta
    20161 gcaatcgtat agaggacagc tgttgcaagt ttatctaatt tcttagcgcg cataattttt
    20221 ctttcctctt tctttcgtaa tcaatttaat cacactgtta aaaactaagc tcatcaagag
    20281 cagtaccaag gccagtgacc agagaacatt attatttaca gttcccatga cagtgttccc
    20341 aattcccata gttaatatag aagttaaagt tgcagctggt gtggtcaagg aagttgggat
    20401 aacagctgag tttccgacaa ccatctggat agctagagcc tcaccaaagg cacgcgccat
    20461 cccaaagacc actgcagtga aaataccaga acgggccgcc ttcaagatca cacgccagat
    20521 agtctgccag cgagtggctc ccatagcgaa actggcttca cgataataac gaggaaccgc
    20581 acgcaagcta tccgttgtca taaaggttac ggtcggcaaa atcatgacaa agaggacgga
    20641 aatccctgac aaaatcccaa aaccagtccc accaaagaca ctgcgaacaa agggaacgac
    20701 gacttgcaag ccaataaatc cgtacactac tgaaggaatc ccaaccagga gttcaatagc
    20761 tggttgcaaa atcttcgccc cttttggtga tacttcggtc ataaaaactg ctgcaccaat
    20821 agcaaagggt gttgcgataa gggctgagag aatggtaacg ataaaggaac ccaaaatcat
    20881 aggaagggca ccaaattctt tactagaagg attccaagtt cctcccaaaa gaaagtcaaa
    20941 gatattcaca ccattgacaa agaaggtcga caagcctttt tgcgctacga aaaccaaaat
    21001 catggccaca aggatgacta tcaaagaaag acaggcaaag gtcaaacctt ttcctaattt
    21061 ctccagacga gaattctttg atggaagcaa cattttctta gctaattctt cttgattcat
    21121 tattgtctcc cttccaacac tgtcacagtt ccggcagcat ctttttcaac cttcatttcc
    21181 ttaatcggaa tatacttcaa tcctttgaca atcccttctt gggtctcatc cgagagaaca
    21241 aaattgagaa attctgcagc taactcattg ggctgcccca atgtatacat atgctcataa
    21301 gaccacaagg gccaattatt actacttata ttttctggac ttaagtcata gccattcaac
    21361 ttcatgcttt tgaccgaatc atctatatag gtaagagata aataagagat agctcctgga
    21421 ctttttgata cgattgattt taccgctcca tttgaatcct gctcctgact ttgcatggca
    21481 gactgacctt ccataatgac agtatcaaag gtagcacgag agccagagcc ggctgcccga
    21541 ttgataacag agatgggtaa gtccttacca ccaacctctt tccaattggt tacctcacct
    21601 atgaagattt gacgaagttg ctctgtcgtt aggttatcaa catcaacctc cttattgaca
    21661 atcagagcca agccagctac cgcgaccttg tggtcaacaa gagcagaagc atcaattccg
    21721 tctttttcct cagcaaatac atctgagttt cctatatcaa ctgccccaga ctgaacctgg
    21781 gacaagcctg taccagaacc tcccccttgg acattgaccg tttttccaac atggatcgtg
    21841 ccaaattcat ctgccgctac ttcaaccaag ggttgcaagg cagttgagcc aacagccgtt
    21901 atggattctc cacgatcaat ccagctagca cagcctacta aacaagccgt cagccaaaaa
    21961 gcgataagag acagagcaag cttttttctt tttttcactg tttttctcct cgaaaataat
    22021 tatgaatact gtgaattttt taagtagttc tttatgagtt gacgcatgaa ttcttaccaa
    22081 atttctgcgc aattgattat ttatataata taggctatat tactctttcc taacctcctt
    22141 ttttcatatg tggataaaat ctcttgtcta tcccttcccc cattgtcacc cattatagtc
    22201 atttcgtgtc tctttttccc ctttttaatg caagggaaat tactcttctt agatgataat
    22261 ccaaaagcta gaaaggtatc tcaaacctct ctactctccc agactagttt acaactaaaa
    22321 gaaaaagatt ctattttatg agaaatctag tttacaagcg gtaagaacgc taataactaa
    22381 acttcttgta ctctttgaaa atctcttcaa accaatgttt tgagctatct atggctagct
    22441 tcctagtttg ctctttgatt ttcattgagt agtaaaacta catgtaatgg caatcaagat
    22501 atcaagaatc atcctactaa aaaaatccat actttcacta taacatagaa taagatattt
    22561 gactagcatt ttcatttgaa tctgaggcct tttggaaaat aatttttcaa aacatttcca
    22621 gtaacctttg caaagcccaa gccattgcct ttaaccaaaa cttggtacca accatttggc
    22681 agactttctg ccagctgaac ggtttctcca gccgcatact tgacaaacgc ttcttggcca
    22741 atttcaaccg actgttcgac ctgactcggt ttcaaggcta aaccaagagc gaaactgggc
    22801 tcaaagcgtt tcttcttaaa agtacccaga tgcagtccat tgcgagcaat cttgagcttc
    22861 cctaaatctg gcaaaagttc tggcaagaga taaagctggt ctccaaaaat ctgcaagata
    22921 cccggtagat tgaccttcaa atggttttgg gcaaattcct gccacaaggc aacttgttca
    22981 cggctgaggt tactcttact tgccttaaat ttaggagctg gattgttacc cttaaactgt
    23041 agatgggcaa caaactgacc ctctccctta aactgatgag ggtacatccg agccgtttct
    23101 ggcaggtcaa taccagctac cattccattg atatgctcta ctggcaacaa gtcaaaatca
    23161 tactcttcca gcaaccaatt gacaatctct tcgttttcct cgggtgccca ggtacaggtc
    23221 gaataaacca gacgaccacc ttcagctaac atggtcactg catcctccag aatttctctt
    23281 tgcaagctag cacattgact cggataatct aagctccaat agtccatagc atcaggttgc
    23341 ttacgaaaca ttccttcacc agagcaaggg gcatcaagaa cgattaagtc aaaatagcct
    23401 ttaaagacct tgaccaagcg gtcggcagat tcattggtca ccacgacatt tgtcgctcca
    23461 aaacgctcca tgttttcaac caaaatctta gcccgtttgc ttgaaatttc attggaaaca
    23521 agtagcccct cccctgctag ataggctgcc agttgagttg atttgccccc cggtgcagca
    23581 gccaagtcca agaccttcat accaggactg ggttgggcta cttgagccac catttgagca
    23641 gcaggttctt gcgaataaac taaacctgta gcatgctcag gcgatttccc tgaaaccttc
    23701 ccatagtggc cccaaggggt ttgagtaatg gcatcagaaa aggaaagttg ctcttctttt
    23761 aagggattga cccgaaaggc cgaaaccgct tcctcctcaa aagaggcaag aaaatctctt
    23821 gcctcatctc ctagtatctc tttatatttt tcaacaaatc cttctggaaa tttcatttaa
    23881 gttcttttcc tttcgtaaat ataggactga atttcctcct gcatctcaag aggcaccatc
    23941 atgaccggct gtctggtttg aaaatcagga gcttcaccaa aaagggtcac aacccgatag
    24001 cccagacttt cccctaaaat actagctgcg gcataatccc atggttgcag ataagtgaga
    24061 taggtcaaca aacgccctga caaaatcttg gcaaaactaa tggccgcact tccatagaca
    24121 cgaacaccaa gaaccgatcg gctcaaatca gccagccccc attcattggt ttccagcata
    24181 ccactattcc ctgcaatgag aaaatctcca agtggtttag ttttaaaagg agctaggggc
    24241 ctatcattta gacaaactgg aaattcccca ccaccgtggt aacaatcccc tttgaccaca
    24301 tcataaatca gaccaaactg tccctgacca ttttcaaaat aagccatcat aacagcaaaa
    24361 tcttcctgct gggctacaaa attattggta ccatcaatgg gatcaatgac ccaaaccttg
    24421 ccctcttgaa ccgaggctcg cagacaacct tcttcagcac aaatcttatc ctcaggataa
    24481 cgggacaaaa tctcaccaac caagagttcc tgaacttctt tgtccagtct ggtcaccaaa
    24541 tctgttggag aggacttggt ttcaacacgc aagtcttcct gcatatggtc aagaatgtac
    24601 tgacctgctt tcttaacaag ctctttagca aattcaaatt tactttccaa gagaaatctt
    24661 tccttcccct ttttctttgg cagcctgaac tgctcggtaa agtgaatagc cactaaccgt
    24721 ttcaaattct cgtcccaaac gtttctcttc gctcttgctt ggcacgaccg ctttgaatcc
    24781 cttataggag tccagtaact ttttagcctc tactttgcct tcataggcag cttcaacatc
    24841 attaaaaaaa gaaagcactg aagcaagttc ttcagtgctc cacgacaaat ctagtgggta
    24901 actatactgt ttgttcatta actaatacca gctctcattc ttgcttcttt tagttcttgc
    24961 ttacgataac tacgagggag aaaagcacga atctcatctt cattaaaacc gatttgcata
    25021 cgcttggcat caataataat tggacgacgc aaaagactag gatactgctc aatcaaatga
    25081 agcaattccg ataccgaaat actctctaca tcaatattca atttttgaaa aatttttgaa
    25141 cgagttgaaa tgatgtcatc agtaccattt tcggtcaagg aaaggatgtg ttgcaattct
    25201 tttcttgtta aaggactggt cataatattg tgttccacaa agggaacctt atgtttttct
    25261 aaccaggcct tagccttacg acatgatgta cagctcggtg atagaaatag tgtaatcatg
    25321 cttttctctt cttatctata ctttgctact tctattatac aaaaaaataa ggcgcttgac
    25381 tagggatttt tagaaaaaaa gcctattttt tcaggaaaaa taggctgttt gcgaacgatt
    25441 gacacaattg gatttggtta attcactctt aacgatggtt ttaaacgata tatatattta
    25501 tatatgtaaa ttaaaaacat ctttcctttc acttcctacg acttttcaga cacagatagc
    25561 caaagaagtt ttcatagagg gcaaaaaaga ggaggaaggc atgaagaaag aaggtctctg
    25621 gcaaaatcat aataacagga tccttggctg gatcaaaaag ccaggtatca tctcccacaa
    25681 agagaatttg atggaaaaga gtaaagaatt ggtcaaaacc aatcaaaact cccccaagtc
    25741 caatcattac aggtaaaact actagagcca ggagactttt tcgataaaga gacaaaaagt
    25801 cctttttcac aatcctattg acaaagacat agaaacttgg cagtgtcact agagctacta
    25861 gctgaaccaa atgaaagaga ttcttgacca ctgcgaaatg gtgcagacca gctgctgacg
    25921 aacgaaaatc aggcatctgt aagacctgac taaaaggatt ggtcagataa ttcatcaaga
    25981 tatgaaaatt gtattgaatg gtttctggtt ttagatagac tcgattcgtt aagtttagcc
    26041 actgaatctc cataggatag aaaatccaag ccagataaat ggtcagaagg atggagaggg
    26101 agaggagaaa gagcatagag ccccaaaaga tcaatttagt tttcatcaaa atcccactcc
    26161 gcaaggctag aaaccacatg tgtcggtgcg attggcaggc cagctacttc ttctgcctta
    26221 gtaaaacctg tcgtcaccaa gagcgttgga atgccattgt caatcccagc ccgaatatca
    26281 gtcaaataat tgtccccaac catgattaac tcttcacgtt ccaaacctaa gtgctcaacc
    26341 gccttgtcca taatgatggc atttggtttt ccgatataaa ccggcttcac tcgtgtcgct
    26401 acttcaagca gcgtaatcag tgagccagca cctggcaaaa gaccgcgttc cgtcgggatg
    26461 ttgaggtcag gattggttcc gataaaatgg gcaccctttt gaatagcaag agttgctgtg
    26521 gcaaattttt catagtcgac ttgccaatcc agaccaacta ccacgtaggc aggtttttcc
    26581 ttgtcttcca cataaccagc cgccttgatg gcttccttga gtcctgcttc tccgacgaca
    26641 tagacggtct tttcaagccc caaatcattc atatagtcga tggttgccaa agtcgctgtg
    26701 tagacagtcg ataggggcgt atcgatatta aaattctgag ccaacatctc cttaacactc
    26761 tctggagtgc gggttgtatt gttggttaca aagagatagg gaatgtcccg cttttgcaat
    26821 tcatgaacaa aagtctctcc agcagggatt cggtctttcc ccttataaat ggttccgtct
    26881 aaatcaatta aatagccttt atatttcatc tatttctccc taagcctttt ttatttcttg
    26941 ccaagtaatg attgcttggg cattgataac cccatcactt gtaatttcat gcttgctttc
    27001 cagtccagtc cgttcaacag ccgatgtaat caccccacct ggtcgaactt ccttgacata
    27061 cttgaggttg attttcttgg gaatatagtg ggtcaaaaaa tccgctccca tgacctcaaa
    27121 aatccagtcc aagtatttac tgttattgac atgaccattc atatccaagt cgtaaaaacg
    27181 aacatggtaa tccttgctga tcggttcttc caaggactca tacttcggtc cacggataag
    27241 ttttttatca aaatcagact ggtaaggagc cacaatctca ggttcaacaa catggacttt
    27301 tcgactgtcg cggtccatga gaacaaaggt cgccatcatg tggatgagct cctgctccgc
    27361 ttcattataa atagtaaagc gacggtagca aaaaagtcga ttgtagctca aggcttccgt
    27421 ttcgatggta atttcttccg caaaacgagg caaacgaacc acctcaatat catattctac
    27481 gataatccag accagattat attcttccaa aatggcctta tcactaactc ccagttcaat
    27541 cgactgcatc cctgaaactt gcagtgacag caaaatcaca tctggaagtt tgatatgacc
    27601 gttcatatca gccatatcaa aaggaatttt cattttcatt tgataagtta agcccatgat
    27661 cctactccaa aataaatcgt tctgctacag tatctcccaa aaagagacct ctctttgtca
    27721 tgcgaacgtg gtcaccctca atctgcatga ggccttgttg aaccaaatct ctgacaattt
    27781 ctccataaag tccagcaaaa gactgtccaa atttttcctc aaatcgcgcc atggaaaccc
    27841 cggatttctt gcggagtccc aagaacattt cttcttccat ttgctccttt tgactcaggt
    27901 gatcttctgt aatacaagca ttgccttcct caaccgcact gagataatga cgaatgggac
    27961 catgattttt atagcgtact ccattgacat aaccagatgc ccctgcacca ataccatagt
    28021 attcagcatt gtcccagtac atgagattat gacgactttc aaaaccgggt ttggagaaat
    28081 tagaaatctc ataatgctca aaacccgctc gctccagctc tgcaatgatg tactcaaaca
    28141 tctccgcttc tagttcctcc ttaggcagag gcaatttccc acgtcgcatc cggttcataa
    28201 agaccgtatg gttttctaaa atcaaactat acaaactcat gtggggaata tccaatccaa
    28261 tggctttagc cacattttcc tttacttgct ccatggtctg accaggcaga gcataaatca
    28321 aatcaatgga gatattgtca aaaccagcca gtttcaggcg atcgatattt tcataaatat
    28381 ccttctccaa atgactgcgc ccaatctttt tcaacatctt atcatcaaag gtctggacac
    28441 ctagcgaaac acgattgaca gccgaatttt tcaaaacagc tatcttatcc gcatccaaat
    28501 cgcctggatt ggcttcaatg gtcaactctt ctaagacaga caagtccaag tttttagtca
    28561 agccattcag taaaacctcc agttgcggag ccgacagggc tgtcggtgtt ccaccaccga
    28621 tataaagggt tgacaacttt tcaatatcat aagaacgaaa ctcttccagc agatgctcta
    28681 aatagctgtc gactggctga tttttgatga agacctttga aaaatcacaa taataacaaa
    28741 tctgggtaca aaatgggatg tgcacatagg ctgacgttgg ttttttctgc atagtaatta
    28801 ttataccaca aagactagat tccagataaa aatcaccatc cccagataca tagtccgttc
    28861 ggagatggtg atggtttatt cttctgttat atcaatcaca atctcttctg agtcatcaag
    28921 agcttcggct ttttcttgcc attgttcctt gagattattt aattgatttt ttgatgcttc
    28981 tgtcgcttga aaagcatagg atttagcttg agcaagtata ctgtccacag tgatttcacc
    29041 tgactcaacc tgttcttttg ttttcagaac aaaatctgta gcctgctcct taacttctgt
    29101 cagtttttca cagacttgct ccttggcata ctccggatct tctctcaaat catctagaaa
    29161 atcttgagcc tgactgcaaa cttgtttgcc cttatcactt gttaaaaaca aggcaagagc
    29221 tgcacctgaa acggttccta aaaggattga ggataattta cccataagga ttctcctttt
    29281 ttattttttg aaaaatttac ttgcaagacg aagagctgac agacttgcac cagtcttgag
    29341 tgtttttgaa ccagctgatg aagctttctt gctcaagaca cgcgcatggt cattgaggtc
    29401 tgaaacagat agagataaat ctgcaacagc actgaagagt ggatcaatcg tagccacctt
    29461 gacattgata tcatctgcca agacattgac cttagccaac aactcattgg tgtgatgcaa
    29521 ggtcacatcc acatctgaag tcaaggtttt aatcgtcttt tctgtttcat cgatgacacg
    29581 accaagcttt tgtacagtaa tgatcagata gaccaaaaag acaatcaaag ctagggcaac
    29641 aagaatatat gcaacttcta acatttagtt ttcctcctct gtaatatagt aaggggcctt
    29701 ctttcgattt tgataaataa cgatcattat accgagaccg ataaggacaa ctgacagcca
    29761 ttgggacact cgaaagccga agaacatgag actatctgtt cgcatacctt cgataaccat
    29821 acgaccgaaa ccataccaaa tcaagtaaaa ggccgtgata tgacctcgtc tgagactctt
    29881 ccatttccgt ctaaaaatca gaatcaaggc aaagccaagc agattccata gagactcata
    29941 aaggaaagtc ggttgacggt agctcccctc aatatacatc tggtcacgga taaagccagg
    30001 tagataatcc agattatcca ctgttgcacc ataagcttct tggttaaaga aattacccca
    30061 acgccccaaa ctttgagcaa tcataacgct aggcgccgca atatctagaa aatcccaagt
    30121 attgatgagt ttacggtcag caaagatata gagcacaaga gccccagtta tcaaaccacc
    30181 gtaaatggcc aaaccaccat tccaaatggc aaaaatctct cctaaattct gactatagta
    30241 atcaaatcgg aaaataacat agtagagacg agctcctaaa atagccaagg gaaaggctac
    30301 taagataaaa tctaaaatat cgtctggtat gatcttcttt ctaggtgctt ctttcatggt
    30361 caaataaacc gcaagaatca agcctgtcac aatacataag gcataccaac gaatggctag
    30421 gggtcctagt tgaatagcaa ttggatcaag cattttgcac ctcatttcga gcgattagac
    30481 ttgtcagtcg ttcgtcgaac aaacgggtcg catcaaagcc catttccttg gcacgataat
    30541 tcatggcagc tgcctcaatc acaacagaga tattacgacc tgttttaact ggaatacgaa
    30601 tacgaggaat ggctacgcca gaaacttcaa gttcctctgc attatttcca agacgatcaa
    30661 aggtcttatg cgtatcgtaa ttttccaaat agacagcaag ctgaacctgt gaagaatcct
    30721 tgacagcact cgcaccgtag agactcataa catcgataat accaacccca cgaatttcaa
    30781 tcaagtgttt caaaatttca gctggttcac cccagagagt aatctcatcc ttggcaaaga
    30841 tatcgacacg gtcatcggct accaaacggt gaccacgttt gacaagctca agacctgtct
    30901 cgctcttacc aattccacta tctccctgaa tcaagacgcc catcccataa atatccatca
    30961 agacaccgtg cacactggta cgttctgcca aacgagaatc cagatagcta gataactctc
    31021 cagataaacg actggtagct gcacggctgg ttaaaatagc aatcttacat tctctagcag
    31081 cctttaacat ctcctctgga accaccaaac cacgggcaac aatgactgct ggtgtctcag
    31141 gtagaaacat ttttttcaaa acttcataac ggctgttgga aggcatgctg atcagataag
    31201 accactcctt catccccaaa agttgaatcc gctctggtgt atagtagtca aaatagcctg
    31261 tcatttcaag accaggtcgc gtaatatccg ctgtattgat ttccttttca agcaattctg
    31321 gttcaccata gacaatgtct agtctaagct tttcaatcac ttcttttact aaaaccgaca
    31381 tcatttcctc cttcaaatga gttcttctct ctattatatc aaattgtagg aggaagtttc
    31441 agatttttgc aggctttgga gtatttattt aaaagaaaaa ggactagatt tctctagcct
    31501 ttcatttcaa ttagaatttt ttacgtttac gagctacttc tgatttacgt ttacgtttta
    31561 cagaaggttt ttcatagaat tcacgtttgc gtgtttcttg aagagtacca gctttagtaa
    31621 ccgcacgttt gaaacggcga agtgcgtcgt caagagattc attcttacgt actactgttt
    31681 tagacatttt tttcactccc ttcaagttca agatctattc tattttacac gtaaaaggaa
    31741 taattgtcaa gaggaaacta agttttttat tcttcataat gattaataat atagttcacc
    31801 atctcctcca tatctgcttc agataaaggt aattcccagt ttgttgtttc cattttaaga
    31861 aaagcatcac tgactttagt tattgtatag caatctttaa aaattggaat ttttttagat
    31921 tgttcaaata agatgcctat tctatcatat aagacgtgaa gttctttact atttggatca
    31981 atatcaaaag actgtaaaga gattaagatg tcatttcctt tatatgacaa atcaatccac
    32041 atgtgatttg ataaatcata tggcctagat aatctactac ccgaggtgta ctttttattt
    32101 cctccgcctc ctgaatattg atttcttgtg gtatatggtt tatcttttga cttagtaaaa
    32161 agaaaattcc cctccttatc tttctttttt gtaatgaagc ctattatact ttttctttct
    32221 tccgttctag cactcttata tgcttctttt atttttaaaa attcttcttt attcatgtgt
    32281 cctcctaaag ttaagttgaa gttttatatg tctaaaacca ctcactccaa agagcaagcg
    32341 gttcttatac tttttatttt tcgagtaagc taagcgcttc agcatctgcg atgatggtta
    32401 catcagggtg attttggagg ctacttgcag gtaggttctc agtcactggg ccagatactg
    32461 ttccggcaat ggcttctgct ttcgactcac cgtaagcaaa aagaataata gacttggcat
    32521 ccaaaatgtt tttaatcccc attgaaatag cttgggttgg gacgtcttca atcttggcaa
    32581 agaagcgtgc attggcttcg atagtagact ggtcaagttc tactacatgc gtttgactgt
    32641 caaatggagt accaggctca ttaaagccga tatgcccatt gcgaccgatt cccaagattt
    32701 ggaggtcaac tggatggtca gccaaaattt ggttgtagcg ttcaacttca gcttcagcat
    32761 tgtccttaac accacgaggc aagaaacttt ctttaaatgg tttttggttg aacaagtttt
    32821 cttgcatgaa gtaacgataa gactgtggat tgtccccatc aaggcccaca tactcatcaa
    32881 ggttgacact ggttagattt gaaaaatcaa ggtcactctc aacaatttcc ttgtaaaatt
    32941 caagtgggct acttcctgtc gcaagtccta gagtttgagc tccattagcc aatttttcct
    33001 tcaaaatctc aaaagcaact tttccacctt gaacttggtt ttcaacttta ataactttca
    33061 tcttttcatc ctccatttct gtctatattt attatatggt atagaccaat ttttgtcaag
    33121 tgaaaacgat ttaatttcta aaaaaagagc gtccaaactt gaaacgtgtt ataatggata
    33181 ggattagaac ttagaaagaa tgaacaaata tatgaataca gctgattttg atttccactt
    33241 gccagaggaa ttgattgccc aaacgcccct tgaaaaacgt gatgcctcca aactcctcat
    33301 cgtcaaccgt gagacaggag aaatgcaaga taaacatttc cactctatta ttgatatgct
    33361 ggaacctggt gatgcccttg tcatgaacga cacccgagtt ctccctgccc gcctctatgg
    33421 tcaaaaagtg gagacaggag gtcatgtgga acttctcctc cttaagaaca ctagtggaga
    33481 cgagtgggaa gttctggcta aacctgccaa acgcctcaag gtcggtactc gtatcagctt
    33541 tggtgatggc cgcctcagcg ctgtcgttac agaagaattg acccacgggg gacgcattgt
    33601 ccgctttgaa taccaaggaa ttttcctaga agtcttggaa agtctgggag aaatgcctct
    33661 gccaccttat atccacgaaa aattagatga ccgtgaacgt tatcaaaccg tctacgccaa
    33721 ggaaagcggc tctgctgcag caccgactgc tggtcttcac ttcaccaaag aactgctggc
    33781 agaaatccaa gctaagggtg ttcatctagt ctatctgact ctccatgtcg gactcggaac
    33841 ctttagacct gtttctgtgg ataatctgga cgaacacgaa atgcactcag agttctatca
    33901 actttctgag gaagctgctg ccacccttcg ctctgtcaaa gaaaatggtg gtcgcgtcat
    33961 cgctgtcgga accacttcta tccgcacctt ggaaactatt ggttccaagt ttgatgggca
    34021 aatccaagca gattctggtt ggaccaatat ctttatcaaa cctgggtatg agtggaaggt
    34081 cgtggatgcc ttctcaacca acttccatct gccaaaatca actctggtca tgttggtttc
    34141 tgcctttgca ggccgtgaat tagtcttaga tgcctaccac catgccatcc aagaacacta
    34201 ccgcttcttc agttttggtg acgccatgtt tatttattga gaaagaattt ctctaattct
    34261 tctaatacca ataaatcgct aagatattat ttcaaagaac atctacaatt gaaactctag
    34321 ctagctgtag aagacgccta gtacattgaa attaaaatgc ttccccctag cttcgaaaat
    34381 attgccatag attgcgttga ctctccaaat tgattcatct atattttatt tcagcttcct
    34441 atattttctt cgctgtttgt aaatcaaaat gcaagacaca tgagtagcac catatttgtt
    34501 actcttatct gtcctctcaa gagactatta tgagttattt cagaatcatt cactactttg
    34561 accctgactc tccttagtct caaaatcaaa gacttatact cttcaaaaat ctcttcaaac
    34621 c
//
LOCUS       DBERDK010000014        33955 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.014, whole genome shotgun sequence.
ACCESSION   DBERDK010000014 DBERDK010000000
VERSION     DBERDK010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 33955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33955
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.014"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            167..427
                     /locus_tag="ACL73L_000667"
     CDS             167..427
                     /locus_tag="ACL73L_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771686.1"
                     /translation="MTGVSNPTTDSARLVLAEAKKAFADDSLTEQGLRDILQTVKDAI
                     ASLESIKESQSATKDGGQTAGKETAGEDESANQTQEISQGIK"
     gene            381..602
                     /locus_tag="ACL73L_000668"
     CDS             381..602
                     /locus_tag="ACL73L_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771687.1"
                     /translation="MSLQIKLKKLAKELSKLLKDSNLETVDKDVLENSQKELQKAVLF
                     LADEKGSEHTEAEVIDNLKEVIAKLKANA"
     gene            921..1850
                     /locus_tag="ACL73L_000669"
     CDS             921..1850
                     /locus_tag="ACL73L_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS0771688.1"
                     /translation="MKKFSKTLRDNWIFLLMVLPGALWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLGIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLTILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
     gene            1864..2787
                     /locus_tag="ACL73L_000670"
     CDS             1864..2787
                     /locus_tag="ACL73L_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGS0771689.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLTGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
     gene            2871..4346
                     /locus_tag="ACL73L_000671"
     CDS             2871..4346
                     /locus_tag="ACL73L_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGS0771690.1"
                     /translation="MKNWKKYAFASASVVALAAGLAACGNLTGNSKKAADSGDKPVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQ
                     NFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     TWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEVLNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDGYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFDTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
     gene            4618..5604
                     /locus_tag="ACL73L_000672"
     CDS             4618..5604
                     /locus_tag="ACL73L_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HGS0771691.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMENLWFKIDEESEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
     gene            5726..6586
                     /locus_tag="ACL73L_000673"
     CDS             5726..6586
                     /locus_tag="ACL73L_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 family protein"
                     /protein_id="HGS0771692.1"
                     /translation="MAKTFFIPNKQSILGEQEILNAKSILALLDGLESHSYDVVYLRQ
                     PLNRLEYIECAIVGQSQFLFKVSYADGQKAYRVDLPDLLTKTDWQIIKSFLDALLAYT
                     GTDIEGLDGFDFEAYFQASIQAYLADPVARFTICQGIFNPIFFSRENLKSFLEADGLA
                     QFEARVRAVQETDAYFARVSFYQDGEGKVHGVYHLAQGVKTVLPREPFVPAAYIEQLV
                     DKEVQWEIYLVQITGDGSKPEDYESIARLDYAKFLEVLPPSFYHQLDANQLEIQPILG
                     QDFKTLAQEK"
     gene            complement(6658..7722)
                     /locus_tag="ACL73L_000674"
     CDS             complement(6658..7722)
                     /locus_tag="ACL73L_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6574 domain-containing protein"
                     /protein_id="HGS0771693.1"
                     /translation="MKQEWFESNDFVKTTSKNKPEEQAQEVADKAEETIADLDTPIEK
                     NTQLEEEVSQAEVELESQQEEKIEAPEDSEARTKIEEKKASNSTEEEPDLSKETEKVT
                     IAEESQEALPQQKATTKEPLLISKSLESPYIPYQAPKSRDKWKEQVLDFWSWLVEAIK
                     SPTSKLETSITHSYTAFLLLILFSASSFFFSIYHIKHAYYGHIASINSRFPEQLAPLT
                     LFSIISILVATTLFFFSFLLGSFVVRRFIHQEKDWTLDKVLQQYSQLLAIPIFLTAIA
                     SFFAFFDSLRFTALLCVISIGIILLASLHIITRPSQASETDSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGDYLRILAFL"
     gene            complement(7785..8696)
                     /locus_tag="ACL73L_000675"
     CDS             complement(7785..8696)
                     /locus_tag="ACL73L_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HGS0771694.1"
                     /translation="MRKWTKGFLIFGVVTTIIGFILLFVGIQSDGIKSLLSMSKEPVY
                     DSRTEKLTFGKEVENLEITLHQHALTITDSFDDQIHISYHPSLSAHHDLITNQNDRTL
                     SLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILQLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNSYILRIEGSRIKNSKLTTPNIVNIFDTVLTDSQLESTENHFHAEN
                     IQVHGKVELTAKDYLRIILDQKESQRINWDISSNYGSIFQFTREKPESRGTELSNPYK
                     TEKTDVKDQLIARSDDNIDLISTPSRR"
     gene            complement(8689..9282)
                     /locus_tag="ACL73L_000676"
     CDS             complement(8689..9282)
                     /locus_tag="ACL73L_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HGS0771695.1"
                     /translation="MTRTEYLTQLELYLKKLPEADRIEAMDYFRELFDDAGVEGEEEL
                     IASLGTPKEAAHEVLSNLLDKKINEAPAQKNNRQILHIALLALLAAPIGIPLGIAILV
                     TLFAILVAALTVILAFFAVSILGIIGGFLFLVESFTILAQAKSAFILIFGSGLLAIGA
                     SSLVLLGISYVARFFGLLIVRLVQFVLKKGKRGNQHA"
     gene            complement(9269..9595)
                     /locus_tag="ACL73L_000677"
     CDS             complement(9269..9595)
                     /locus_tag="ACL73L_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HGS0771696.1"
                     /translation="MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSREFQGRMRKYYSLTNGGIEQLLTLKDEWTLYTDTINGIIEGS
                     IRHDKN"
     gene            complement(9734..10900)
                     /locus_tag="ACL73L_000678"
     CDS             complement(9734..10900)
                     /locus_tag="ACL73L_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
                     /protein_id="HGS0771697.1"
                     /translation="MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSL
                     GVLTSLPLLMFTLFSPFSTQLAQKIGLEHLFTYSLFFLTIGSLIRLINLPLLYLGTLM
                     VGASVAVINVLLPSLIQANQPKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGL
                     ILLLTLLCLATFLVWLPNHRYNHRLAPQTKQKSQIKVMRNKQVWAIIIFSGFQSLIFY
                     TVMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVS
                     LAGVVGISMLFFPINNFIYWLTIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGL
                     SQTGGYILAAFGPTLFGYSFDLFHSWVPAVAALLLIYILMTVALFTVDRADKIL"
     gene            10958..12118
                     /locus_tag="ACL73L_000679"
     CDS             10958..12118
                     /locus_tag="ACL73L_000679"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGS0771698.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIEWWKEKFRRVVVTQNVESLLVSIV
                     ISAYNEEKYLPGLIEDLKNQTYPKENIEILFINAMSTDGTTAIIQQFIKEDTEFNSIR
                     LYNNPKKNQASGFNLGVKHSVGDLILKIDAHSKVTESFVMNNVAIIQQGEFVCGGPRP
                     TIVEGKGKWAETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNE
                     QLGRTEDNDIHYRIREHGYKIRYSPSILSYQYIRPTFKKMLHQKYSNGLWIGLTSHVQ
                     FKCLSLFHYVPCLFVLSLVFSLALLPVTFVFITLLLGAYFLLLSLLTLLTLLKHKNGF
                     LIVMPFLLFSIHFAYGLGTIVGLIRGFKWKKEYKRTIIYLDKISQINQNMLQ"
     gene            12157..14007
                     /locus_tag="ACL73L_000680"
     CDS             12157..14007
                     /locus_tag="ACL73L_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HGS0771699.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLAFLFYQLMIGFWGLNASISRYSKITDFMKIFFGVTASSVLS
                     YSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVY
                     HELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVAKAVDEAKVSKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE"
     gene            complement(14093..14440)
                     /locus_tag="ACL73L_000681"
     CDS             complement(14093..14440)
                     /locus_tag="ACL73L_000681"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771700.1"
                     /translation="MKIKEQTRKLAAGCSKHCFKIVDGTDEVSSKHCFKVVDGTDEVS
                     SKHCFEVADRTDEVSSKHCFKVVDGTDEVSSKHCFEVVDRTDEVSSKHCFEVVDRTDE
                     VSNHIRQGDVDVI"
     gene            complement(14490..15122)
                     /locus_tag="ACL73L_000682"
     CDS             complement(14490..15122)
                     /locus_tag="ACL73L_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HGS0771701.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIR
                     GFMGPPLESSFATCLSKDQISEAMQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPL
                     YITTTKNTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLAPEQAIII
                     GDTKFDMLGARETGIQKLAVTWGFGEQADLLNYQPDYIAHKPLEVLAYFQ"
     gene            complement(15144..16016)
                     /gene="sdaAA"
                     /locus_tag="ACL73L_000683"
     CDS             complement(15144..16016)
                     /gene="sdaAA"
                     /locus_tag="ACL73L_000683"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HGS0771702.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIENGKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAT
                     TPTGRRLQKEIFGE"
     gene            complement(16025..16696)
                     /gene="sdaAB"
                     /locus_tag="ACL73L_000684"
     CDS             complement(16025..16696)
                     /gene="sdaAB"
                     /locus_tag="ACL73L_000684"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HGS0771703.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHN
                     VNFFK"
     gene            16938..17525
                     /locus_tag="ACL73L_000685"
     CDS             16938..17525
                     /locus_tag="ACL73L_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HGS0771704.1"
                     /translation="MKSITKKIKATLAGVAALFAVFAPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVDQELVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVAETPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNG
                     DYSAENQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     gene            complement(17577..17903)
                     /locus_tag="ACL73L_000686"
                     /pseudo
     CDS             complement(17577..17903)
                     /locus_tag="ACL73L_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268160.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            18349..18600
                     /locus_tag="ACL73L_000687"
     CDS             18349..18600
                     /locus_tag="ACL73L_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HGS0771705.1"
                     /translation="MVCSILASTSVLAVWVDGGQWNYGVGWSGNFGYSDYLHSTRSHT
                     ATVKDGNKFSKDRAEAEAWARASLTKIPPTGMEYFYGFE"
     gene            18652..20760
                     /locus_tag="ACL73L_000688"
     CDS             18652..20760
                     /locus_tag="ACL73L_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HGS0771706.1"
                     /translation="MKKISNFCMLLLLLCTTFFVFNVNYTREVVRIQEMGKTVDSLDL
                     YLKDINEPAASVLRFFEDVSKEYKVSIIKTDSGDEVVKSGVFDKDTFPYQEFGISSLD
                     FTTDGEGVYSNKEISNKLGTIPTFLKAKPIQLMTFQTYIKDTSRSLNGRYTITSTQEM
                     DKDRIVQKWSDFFKIDQATLLEPTYKSAVEVINRDLLLSAIVFVLAILLLVLVTVYQP
                     MMEMKRVGVQKLLGFQDRAVLADVVKGNLYLLLGGALVINLGVFFLLDYKPKDLFPML
                     WLSHFLLLQLYLFISWLTYLLIQKMTISSLLKGFSSFKFGLIFNYVMKIGTTILLTAL
                     LIGVGRSLEQENKELAYQQQWVSQGNYLTLETFKLNDNLWQEELAGSGKSTDYFYRFY
                     QDLVEKTQAGYVQSSSLPVKNFVQSEQIQQYQLTDTVDVYYANRNFLKSKGFKLPDTG
                     IKKVILMPASTKGEEDKNQLLGKLIAFHSMKYEEQQKRTIEEMDVEIAYYEGDWSFFP
                     YSDKRKENLSNPIISLVNDSDMMWDEKASLSTTGLNNPIKIENTVQHQKEITELVEKL
                     SDGNYLKFSSIQAIQQEKVDSYRDAVRNFNLLFALFGLLSMMISYFLLVTTFLLKRRD
                     IITKKFMGWKLVDRYRPLLVLLLLGYSFPLLVLIFFAHAFLPLLLFAGFTCLDILFVL
                     GLASRMEKRSLVELLKGGIL"
     gene            20757..21398
                     /locus_tag="ACL73L_000689"
     CDS             20757..21398
                     /locus_tag="ACL73L_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0771707.1"
                     /translation="MIELKNITKTIGGKVILDNLSLRIDQGDLVAIVGKSGSGKSTLL
                     NLLGLIDGDYSGRYEIFGQTNLAVNSAKSQTIIREHISYLFQNFALIDDETVEYNLML
                     ALKYVKLPKKDKLKKVEEILERVGLSATLHQRVSELSGGEQQRIAVARAILKPSQLIL
                     ADEPTGSLDPENRDLVLKFLLEMNREGKTVIIVTHDAYVAQQCHRVIELGEGK"
     gene            21604..22410
                     /locus_tag="ACL73L_000690"
     CDS             21604..22410
                     /locus_tag="ACL73L_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGS0771708.1"
                     /translation="MNKMKKVLMTMFGLVMLPLLFACSNNQSAGIEAIKSKGKLVVAL
                     NPDFAPFEYQKVVDGKNQIVGSDIELAKAIATELGVELELSPMSFDNVLASVQSGKAD
                     LAISGVSKTDERSKVFDFSTPYYTAKNKLIVKKSDLATYQSVNDLAQKKVGAQKGSIQ
                     ETMAKDLLQNSSLVSLPKNGNLITDLKSGQVDAVIFEEPVAKGFVENNPDLAIADLNF
                     EKEQDDSYAVAMKKDSKELKEAVDKTIQKLKESGELDKLIEDAFKASIEK"
     gene            22427..22819
                     /locus_tag="ACL73L_000691"
                     /pseudo
     CDS             22427..22819
                     /locus_tag="ACL73L_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269502.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase domain-containing
                     protein"
     gene            complement(23135..23461)
                     /locus_tag="ACL73L_000692"
                     /pseudo
     CDS             complement(23135..23461)
                     /locus_tag="ACL73L_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268160.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            24212..24583
                     /locus_tag="ACL73L_000693"
     CDS             24212..24583
                     /locus_tag="ACL73L_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0218 family bacteriocin-like peptide"
                     /protein_id="HGS0771709.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGAIYIPRWAITGAITGAAYAAL
                     AAAGGGGLQLVLASYGLRSALVAGIVKGLGVLGIHIGNAFANTVIRSIASAGIGAGAD
                     WIFTNIIDGWDGRRDNQLRIG"
     gene            24591..24803
                     /locus_tag="ACL73L_000694"
     CDS             24591..24803
                     /locus_tag="ACL73L_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771710.1"
                     /translation="MLIVLISVIVAFLYHKITEYVNNRKIDIVIFLSLLISHFIWNYY
                     FLPIEILIAISGANLLLSFLKKLQTK"
     gene            25177..25302
                     /locus_tag="ACL73L_000695"
     CDS             25177..25302
                     /locus_tag="ACL73L_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771711.1"
                     /translation="MATITNALNIAATVAEVFSLGGAIAYGLDIVDGKFDGYLWA"
     gene            25283..25529
                     /locus_tag="ACL73L_000696"
                     /pseudo
     CDS             25283..25529
                     /locus_tag="ACL73L_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513491.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            25686..26744
                     /locus_tag="ACL73L_000697"
     CDS             25686..26744
                     /locus_tag="ACL73L_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771712.1"
                     /translation="MKDDQKYLLAGLYSLLVAIFYFPLIESKGIFVSILMAVLLLYLI
                     YFIATVIHIVIIKFIRKKSFKYLVLYPFTYDGSWRFQPINLLYFPEMVRDVIPINLVQ
                     EYCQGQPYGLLKKMLKRIRLSREISLLLATIIVYFFTHRILPLSVFTFIFSYILLFAQ
                     SYLGGNTAWIGNRRLIIDDEFEKILLSKSYIKEISSARYSEYLTCEYKNFTPIILLAI
                     FENLLDSYLLQNQSEVDLDIFYKVLPLLYKEKYTMGFNYFVSLNYLLYKVGFLGIIYD
                     NEALRDLSKQYLNKNISELQDGSFEGGIQDAVASKQIVVINEFIACLNSRCMPSQYDR
                     FFYKDRPYIFSRKSPIKG"
     gene            27059..28036
                     /locus_tag="ACL73L_000698"
     CDS             27059..28036
                     /locus_tag="ACL73L_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771713.1"
                     /translation="MKNKRYFFDTILMILLLISTIFCVSPVFIKLDILGTPSHAILTF
                     VLAIPLFYILSQCLHTLLLLVSSIFCKLRPIYFYFIFVIIIGARKYYRILFHQLMGFS
                     PGVAVFYKESQTTKNLFKFYYFLYFTTLISYYFFFTFVYDKPLLLPLIPFSIIIALVQ
                     KLYRIENQQLFLLKSKVLTILESKKNCEFNLQDYHEIWKLQSKSELPCVALSYISLIK
                     PYLSESVREQIDLLEVKRFKKINHPISLYGMLDVIKLNLYLRHYNEKNKYESMLKKIL
                     EVRPDFVLIEQNIDDSLNSSQPLSLSLAISEIQLLLEVYMGIKHVSIRR"
     gene            28413..28787
                     /locus_tag="ACL73L_000699"
     CDS             28413..28787
                     /locus_tag="ACL73L_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0224 family bacteriocin-like peptide"
                     /protein_id="HGS0771714.1"
                     /translation="MELVLPNNYVALEQEEMMYLDGGFSILRWPVATAINIAFNGVLG
                     GGAISLVRNYIRNYGLRRVTSAIAGAAARYVGVRVANRVAGFALSAINGFAAWMSIGD
                     AITTIWANNDVNRRDPNLNALW"
     gene            28794..28976
                     /locus_tag="ACL73L_000700"
     CDS             28794..28976
                     /locus_tag="ACL73L_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771715.1"
                     /translation="MGIKMEKIFVIIFFVCLFISSITFLAYDFVSEEIKKLIIWMNVV
                     FLILIIAMIIYPKLRK"
     gene            28995..29897
                     /locus_tag="ACL73L_000701"
     CDS             28995..29897
                     /locus_tag="ACL73L_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771716.1"
                     /translation="MIRKLIIFLLMLPLFVLWLELHLLVSNLQLNLEIPLDFVVSTSL
                     AFCSLILSKIVLDILYALKDLYKKEALITIFPFIFIGRKKVNVRFSPYFSFHRKSLSP
                     DDLRSRIIWSFILEIAIILVFILKIPFAIIMLTTIFFWTIMDINHLVFNKTEFLFNQN
                     KWEKEDSFESDLTKTLKDKIQKSELSYSDLMSLLLYDAMNQSTFLTDSELFEDILKKI
                     EDSHNTLLCTGLVELLLYEISISNNNNWSNKVDKIRIQLIRINQLDFFYYTSWLRQNF
                     DFCMNREYHKMKSRKLLLSNKKIV"
     gene            29953..30192
                     /locus_tag="ACL73L_000702"
     CDS             29953..30192
                     /locus_tag="ACL73L_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HGS0771717.1"
                     /translation="MELVLPNNYVVIDEEVMMYLDGGAYLSKRACQGICAAAGVLATA
                     AGKIAYCIGYGVLNRGCDINGNPYPWDGFISATVR"
     gene            30189..30917
                     /locus_tag="ACL73L_000703"
     CDS             30189..30917
                     /locus_tag="ACL73L_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771718.1"
                     /translation="MSNVDKIRKIHIIVCWVYIFLSFRAIINDTEYFLLIFLAFIYSI
                     VSLPLYSVKNKIVSICLAINSILLISFPILINKFFPESFLTYTVLISVFILELAIFHL
                     IGEDFATKLTNEYKKISQFRSKVSQSPWIKYLEISSFILTIFPSILYGTVDNRVLALI
                     FLIKICVDTTIKFLFIRLFDTSTLMKRRIFFLFALDVIAYLFLGYLLVIQKAGYLFSV
                     LLLFSNFSVPFIKAKEYELFKNSK"
     gene            31396..31593
                     /locus_tag="ACL73L_000704"
     CDS             31396..31593
                     /locus_tag="ACL73L_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771719.1"
                     /translation="MKHFFAGIGEIKLVGYLLYICVGLAPLFHIYVIGSELSFVKIVL
                     SILGVIFVSMLTIARIYRTFL"
     gene            32050..32289
                     /locus_tag="ACL73L_000705"
     CDS             32050..32289
                     /locus_tag="ACL73L_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771720.1"
                     /translation="MKLLDCILDYQERFNGKTCQVSTNYKYLEIFKVNFCLTDLHHLF
                     DLYKITRDYASQTKPAIQAGVFILEDFRNILCTMM"
     gene            32274..32552
                     /locus_tag="ACL73L_000706"
     CDS             32274..32552
                     /locus_tag="ACL73L_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771721.1"
                     /translation="MYNDVIERISLYEFIGDIFYSKIISCCIVARDLSKNTMKLDVIF
                     FEDKNKRSAVLGLRRDKSGVFKSVTLHFTSAKKYAKVRKTDVKEMKWL"
     gene            32838..>33955
                     /locus_tag="ACL73L_000707"
     CDS             32838..>33955
                     /locus_tag="ACL73L_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HGS0771722.1"
                     /translation="MNKKKMILTSLASVAILGAGFVTSQPTFVRAEEAPQVVEKSSLE
                     KKYEEAKAKADTAKKDYETAKKKAEDAQKKYDEDQKKTEDKAKAVKKVDEELQKANLE
                     LQKAHVKYQKAQRELSESPDRRKSAARKKLADALSYIGEVELKQKEAEANFNTEQAKV
                     IPKETELAVTKQKAEEAKKEAEVAKKKFDKAAQEVEVAKKEVEAKELEIEKLQDEIST
                     LEQEVATAQHQVDNLKKLLAGVDPDDTEAIEAKLKKGEAELNAKQAELAKKQTELEKL
                     LDSLDPEGKTQDELDKEAAEAELDKKADELQNKVADLEKEISNLEILLGGADPEDDTA
                     ALQNKLATKKAEFEKTQKELDAALNELGPDGDEEETPAP"
BASE COUNT        10652 a         5596 c         7010 g        10697 t
ORIGIN      
        1 gatttgaagg cgtttatgat atttagctgt acgagagtct tttaaaagtg ttttgatggt
       61 ttggatttct tctttagttg atttcatatc actgttatat aatacttttt gattttagtc
      121 tagtattagt aagcagactc aaaccttaga aaatcttttg agatcattga caggtgtatc
      181 gaatcctacc acagattcag cacgtctggt tttggcagag gctaagaaag cttttgcaga
      241 tgatagtttg acagagcaag gtttacgtga tatcttgcaa acagtcaaag atgccattgc
      301 ttccctagag tccattaagg aaagccagtc agcaactaag gatggaggac aaacagcggg
      361 caaagaaaca gcaggagaag atgagtctgc aaatcaaact caagaaatta gccaaggaat
      421 taagtaaact tcttaaagac tcaaaccttg aaacagtaga taaggatgtc cttgaaaact
      481 ctcaaaaaga acttcaaaaa gcagttttgt tcttggcaga tgaaaaaggc tctgagcata
      541 ctgaagcaga ggtgattgat aatcttaaag aggttattgc taagttgaaa gcaaacgcct
      601 aataacatat taaaaatctc cagattagga actatcgctg agttccctag tctggagatt
      661 tttcagtatc ccccgttgtt ggatgtttat aacagagtga cgccatcact gggctaatag
      721 agataaaata aacaattgat ttaggacatt tgtttgatag tggtgataaa gttttaaatc
      781 tgctacataa tttgtggagt tttttctata attaggagtt taacctaacc tacaaatagg
      841 agtatactaa taatgtaatc gttatcaaaa gtctaaaaaa gaatttttag atggatatca
      901 aaataaaaag ggaggaaatt atgaaaaagt tttcaaaaac attgagagac aactggatct
      961 ttctcttgat ggttttgcca ggggcactct ggttgattct attcttttac attccagtat
     1021 ttgggaacgt ggttgccttc aaagactacc acatgaccag taatggtttc atagatagta
     1081 tcataaatag taaatgggtc ggactcgata atttcagatt cttatttagt tcaagagacg
     1141 cctttattat cacacgaaat actgtcctct acaatcttgg ctttatcttt ctaggtttag
     1201 ttgtatctgt agggattgcc attatcctca gcgagctccg ttctaagaga atggtgaaga
     1261 ttttccaaac ttctatgttg ttcccttact tcttgtcttg ggttatcatc agtttcttta
     1321 cagatgcctt cctaaatatt gataaagggg tgttcaatca tctattggaa agtcttggtc
     1381 tcaaagaagt caatttttac gctgacctgg gcatctggcc ctatctccta cttttcctag
     1441 gtatttggaa aggctttgga tatagcagtg tcatgtacta tgcgacgatc atgggaattg
     1501 atccaaccta ctacgaagca gcgacagtgg acggagctag caagtggcaa cgtattcgca
     1561 acgtaaccat tcctcagttg actccgcttg taactgtatt gaccatcctt gcagtcggaa
     1621 atatcttccg cgcagacttc ggtctcttct atcaaatccc acacaatgct ggtcagcttt
     1681 acaatgtaac caacgttttg gacgtatatg tctttaatgg tttgactcag acagcagata
     1741 tcggtatggc tgcagcagcc ggtctttacc aatccgttgt tggtttgatt ctggttatcc
     1801 tatcaaactt gcttgcaaga cgagtcgatc caaactcagc tttgttctag aaaggaggag
     1861 aatatggcaa aaaagaaaat taaaaaagaa aaaattgata atgtcggcat tcactccttc
     1921 agtaagaaag cagatatctt cttcagtatc atttcgggtt tgattgccct ctcttgtatc
     1981 ctaccttttg tattcgttat cattatttca gtgacagacg agaaaagcct cctccaatac
     2041 ggctatagct tcttcccatc ccaatttggc ttagacggtt ttgagttttt ggcacagttt
     2101 aaggataaaa tcctacaagc cctcttcatc tctgtctttg taaccgtagt tgggacattg
     2161 acaaatgtct ttatcacaac aacctatgcc tacgctattt cacggacaac ctttaagtat
     2221 cgcagatttt ttaccatctt cgtccttctc agtatgttgt tcaacgctgg tttggtacca
     2281 ggttatatca tggtgacccg tgtacttcag cttggggata ctgtttgggc cttgattgtt
     2341 ccaatgcttc tctcaccatt taacatcatc ttgatgcgtt ccttcttcaa gaagaccatt
     2401 ccagaagcca ttctagaatc ggctcgtatc gatggtgcca gtgaagcccg gattttcttc
     2461 caaatctgtt tgccattgtc actaccaggt atcgcaacca tcacgctctt aacagctctt
     2521 ggtttctgga atgactggtt caacgccctt ctttacatca agagtgacaa cttgtatcca
     2581 ttgcaatatt tgctcatgca aatccaacaa aatatggact acattgccaa agcggtcggc
     2641 ttgactggtc aactgggagt tgctctaccg aaagaaacag gtcgtatggc catggttgtg
     2701 gttgcaaccc ttccaatcgc gattttgtat ccatttttcc aacgctactt tgtaaaaggt
     2761 ttgactatcg gtggtgtgaa agaatagcac ttgtttccaa acttcccttg cgtgaagtta
     2821 agatcattac attgtttata agtttaaaaa taaaaaaagg agtttttatc atgaaaaact
     2881 ggaaaaaata tgcttttgca tctgctagtg tagtcgcttt ggctgcaggt cttgctgctt
     2941 gtggaaattt gacaggtaac agcaaaaaag ctgctgattc aggtgacaaa cctgttatca
     3001 aaatgtacca aatcggtgac aaaccagata acttggatga attgttagca aatgccaaca
     3061 aaatcatcga agaaaaagtt ggtgccaaat tggatatcca ataccttggc tggggtgact
     3121 atggtaagaa aatgtcagtt atcacatcat ctggtgaaaa ctatgatatc gcctttgcag
     3181 ataactatat tgtaaatgct caaaaaggtg cttatgctga cttgacagaa ttgtacaaaa
     3241 aagaaggtaa agacctttac aaggcacttg acccagctta catcaagggt aatactgtaa
     3301 acggtaaaat ttacgctgtt ccagttgcag ccaacgttgc atcatctcaa aactttgcct
     3361 tcaacggaac tctccttgcc aaatatggta tcgatatttc aggtgttact tcttacgaaa
     3421 ctcttgagcc agtcttgaaa caaattaaag aaaaagctcc agacgtagta ccatttgcta
     3481 ttggtaaagt tttcatccca tctgataact ttgactaccc agtagcaaac gggcttccat
     3541 tcgttattga tcttgaaggc gatactacta aagttgtaaa ccgttacgaa gtgcctcgtt
     3601 tcaaagaaca cttgaagact cttcacaaat tctatgaagc tggctacatt ccaaaagatg
     3661 tcgcaacaag cgatacttca tttgaccttc aacaagatac ttggttcgtt cgtgaagaaa
     3721 cagtaggacc agctgactac ggtaacagct tgctttcacg tgttgccaac aaagatatcc
     3781 aaatcaaacc aattactaac ttcatcaaga aaaaccaaac aacacaagtt gctaactttg
     3841 tcatctcaaa caactctaag aacaaagaaa aatcaatgga agtgttgaac ctcttgaata
     3901 cgaacccaga actcttgaac ggtcttgttt acggtccaga aggcaagaac tgggaaaaaa
     3961 ttgaaggtaa agaaaatcgt gttcgcgttc ttgatggcta caaaggaaac actcacatgg
     4021 gtggatggaa cactggtaac aactggatcc tttacatcaa cgaaaacgtt acagaccaac
     4081 aaatcgaaaa ttctaagaaa gaattggcag aagctaaaga atctccagcg cttggattta
     4141 tcttcaatac tgacaatgtg aaatctgaaa tctcagctat tgctaacaca atgcaacaat
     4201 ttgatacagc tatcaacact ggtactgtag acccagataa agctattcca gaattgatgg
     4261 aaaaattgaa atctgaaggt gcctacgaaa aagtattgaa cgaaatgcaa aaacaatacg
     4321 atgaattctt gaaaaacaaa aaataatcag ctaaattgat ttcgtgtatt cattcctaac
     4381 tcctaaaata agaatcaccg ctttcctcag gttggtctgg ggagggcagt ggtttttttg
     4441 gcacaaggat atagatatcc atgttttctc ttgtccttat tttaaccatt caggaaattt
     4501 cgagaaccct ctacaatttc cagtcaaata aattgcattc gttttctcaa gcaggtatac
     4561 tagtatggat aaataaaaaa tttagaaaat ttaagaatag aaaagagaac aaatcttatg
     4621 gcaaaagata ttcgtgtctt actttactac ctctatactc caattgaaaa tgcagagcaa
     4681 tttgctgcag accacttggc tttctgtaaa tcaatcggcc ttaaaggccg tatcctagtc
     4741 gctgacgagg gaattaacgg aacagtttca ggtgactatg aaacaactca aaaatacatg
     4801 gactacgttc acagccttcc aggaatggaa aatctctggt tcaagattga cgaagaaagt
     4861 gaacaagcct tcaagaagat gtttgttcgc tacaagaaag aaattgtcca ccttggtttg
     4921 gaagacaacg actttgacaa tgacatcaac ccacttgaaa caacaggtgc ttacttgtct
     4981 ccaaaagagt tcaaagaagc gcttcttgat aaagataccg ttgtccttga cacacgtaac
     5041 gattatgagt acgacctagg acatttccgt ggggctattc gcccagatat tcgcaacttc
     5101 cgtgagttac cacaatgggt ccgtgataac aaggaaaaat tcatggacaa gcgtgtcgtg
     5161 gtttactgta caggtggcgt tcgctgtgag aaattctcag gctggatggt ccgtgaaggc
     5221 tacaaagatg tcggccaatt gcacggagga atcgcaactt acggtaaaga cccagaagtt
     5281 caaggtgagc tttgggatgg gaaaatgtac gtctttgacg agcgtattgc cgttgatgtc
     5341 aaccatgtca acccaaccat cgtagggaaa gactggtttg atggaacacc atgtgaacgt
     5401 tatgtcaact gtggaaatcc cttctgtaac cgtcgtatct tgacatcaga agaaaatgaa
     5461 gacaagtacc ttcgtggatg ctcacacgag tgtcgtgttc acccacgtaa ccgctatgtt
     5521 tcaaaaaatg aattgacaca agctgaagtg attgagcgcc tagccgctat cggtgaaagc
     5581 ttggatcagg cagctactgt ataagatcaa acagtcctca ggggctgttt ttctatgctt
     5641 tgtactcaaa aatctaaagt ttgtttttgt atctttcagg aaaataaggt atactgtatg
     5701 taaacgattt caaaggagtc cagttatggc aaaaacattt tttattccaa ataaacagag
     5761 cattttagga gaacaagaga ttttgaatgc caagtcgatc ttggccttgc tagacggttt
     5821 ggagtcacat agctatgatg tagtctatct tcgtcagcct cttaatcgtc tcgagtatat
     5881 tgagtgtgcg atagtggggc aatcacaatt tctctttaag gtcagttatg ctgatggtca
     5941 aaaggcttac cgtgtcgatc ttcctgacct actaacaaag acagactggc agattatcaa
     6001 gtcattttta gatgctttgc ttgcttatac agggactgat attgaagggc tagatggttt
     6061 tgattttgaa gcttatttcc aagcaagtat tcaagcctat ctagcagacc ctgtagctcg
     6121 ttttacgatt tgccaaggaa tttttaatcc tattttcttt agtcgtgaga acttgaaaag
     6181 ctttttagag gcagatggct tggctcagtt tgaagcgcgt gtgcgtgcgg ttcaagagac
     6241 agatgcctac tttgcaagag tttccttcta tcaggatgga gaaggaaaag tgcatggcgt
     6301 ttaccattta gctcaaggag tcaagacagt tttaccgaga gaaccgtttg ttcctgcagc
     6361 ctatattgag caattggtgg ataaggaagt ccagtgggag atttacttgg ttcaaatcac
     6421 aggagacggc tctaaaccag aagactatga atccatagct cgcttggact atgcaaaatt
     6481 cttagaggtg ttacccccat ctttttacca ccagctagac gccaatcaat tagaaataca
     6541 acccatccta ggacaagatt ttaaaacatt agcacaagaa aagtaaagca gaagcaggtc
     6601 aatcgacttg cttttttgac atagaaaaaa tcctgccaag gatgacagga ttgaatttta
     6661 aagaaaggcc aagatacgaa gataatctcc aatcagtgcc acttcagcta caaagaagag
     6721 gaggataata actccgttca caaggacaga caagaataat tgatagaagg agtcggtttc
     6781 acttgcttga cttggtcttg taatgatatg gagactggca agcagaatga ttccaatgct
     6841 aattacacac aagagagctg taaatcgtag gctatcaaag aaagcaaaga aactagcaat
     6901 agcagtgagg aagattggaa ttgccaagag ttgactatat tgttggagaa ccttgtctag
     6961 cgtccagtcc ttttcctggt ggataaatcg tctcacaacg aaactaccca agaggaatga
     7021 aaagaagaag agtgttgtcg ctactaggat agagatgata gaaaaaagag ttaaaggagc
     7081 tagctgctca gggaagcgac tgttaatgct tgctatatgt ccatagtaag catgtttgat
     7141 gtgatagata ctaaagaaaa aggaagatgc agaaaacaga atgagcaaga gaaaggctgt
     7201 gtaactgtgt gtgatacttg tttccaactt acttgtagga gatttgatcg cttccactag
     7261 ccaagaccaa aaatcaagca cttgctcttt ccatttatcc ctagattttg gagcttggta
     7321 ggggatataa ggactttcta aagatttact gataagaagt ggctctttcg tagttgcttt
     7381 ttgctgagga agagcttctt ggctctcttc agctatagtg actttttctg tttctttaga
     7441 aaggtctggc tcttcttcag tagaattaga tgccttcttt tcttctattt ttgttctcgc
     7501 ttcactgtct tcaggagctt caattttctc ttcttgctgg ctttccaatt cgacttcagc
     7561 ttgagagact tcctcctcta actgagtatt tttttcaatt ggtgtatcga gatcggctat
     7621 cgtttcttca gccttgtctg caacctcttg agcttgctct tcaggcttgt tcttgcttgt
     7681 tgtttttaca aaatcattac tttcaaacca ttcttgtttc atggtagaac ctccttttta
     7741 gttagataaa tatgctttca tagtagcaga tgtaagcgtt tttgtcaacg tctgcttggt
     7801 gtggatatta gatcaatatt atcatcagat ctcgcaatga gttgatcctt gacatcggtt
     7861 ttttcagttt tgtaagggtt gcttaattcc gtacctcttg attcaggctt ttctcttgtg
     7921 aattggaaga tagaaccata gttgcttgag atgtcccagt taattcgttg gctttctttc
     7981 tggtctagga tgattctgag ataatctttg gcagtcagtt caaccttgcc atggacttgg
     8041 atattttcag cgtggaagtg attctctgtt gactctagct gactatctgt aagaactgta
     8101 tcaaagatat taacgatatt gggcgttgtg agtttactgt ttttgatacg acttccttca
     8161 attcggagga tatagctgtt tgtattgagg gtcgcatttt caaggctagc atttatgatg
     8221 gtggtttgtc cgcgattggc tgagatgttg atccctttta gagttctccc ttttggtagc
     8281 tggagaataa cttcttcaaa acgactagag tagctacttg cgatatgaag aatcccacca
     8341 attccagaag agagaaacgg agtttcagac agtttcttat cagtgagact cagagttcta
     8401 tcgttctgat tggtgataag atcatggtga gcagaaagag atggatggta agaaatgtgg
     8461 atttgatcat cgaaagagtc tgtgatggtg agcgcgtgtt ggtggagagt aatttctagg
     8521 ttttcgactt ccttgccaaa ggttagcttt tccgtacggc tatcatagac aggttctttg
     8581 gacatggaaa gtaggctctt aatcccgtca gattggatac ctacaaaaag caggataaag
     8641 ccgataatgg tagtcaccac accaaagatg agaaatcctt ttgtccattt acgcatgctg
     8701 attacctctc tttccttttt taagaacaaa ttgtaccaga cgaacaatga gtagaccgaa
     8761 gaagcgagct acataggaaa tgccaagtaa aactagcgaa gaagcaccga tagccagtaa
     8821 accagaacca aaaatcaaga taaaggctga tttggcttgg gcgaggatag tgaaactttc
     8881 aactaaaaat aggaatccgc cgatgatacc cagtatggaa actgcaaaga aagccagaat
     8941 gacagtcaaa gcggctacaa ggattgcgaa cagggtcacg aggatggcga ttcccagagg
     9001 aatgccgata ggtgctgcaa ggagggctaa caaggcgata tgtaaaattt gtcggttatt
     9061 tttttgagcg ggtgcttcat tgattttttt atcgagaaga ttggatagaa cttcgtgggc
     9121 cgcttctttg ggagttccca aactagcgat gagttcttct tctccttcga ctccagcatc
     9181 gtcaaagagc tctctgaaat agtccatggc ttcgatacgg tcagcttcag gtagtttctt
     9241 gagatagagt tctagctgag tcaggtattc agttcttgtc atggcggata ctcccttcta
     9301 tgatgccatt gatggtgtct gtatagagtg tccattcatc ttttagggtc aagagctgct
     9361 ctataccacc gtttgtcaag gagtagtatt tgcgcatgcg accttggaac tctctagaat
     9421 aggttgtcag aaagctattg ccttccaatt ttttgagaat gggatagagt gtggattctt
     9481 tgatattagc gatcagctta atggtttggc taatctcata accataagaa tcaccctgct
     9541 ccagtacagc caagatgaga aattcaatca aggcagagga tgttggaaag tacatgggaa
     9601 acctcctttt ctaatgtgta agatttttat atataatttt tctacacata cattgtacat
     9661 ctaaaagaaa gccctgtcaa gagaaatgtg taaaattttt atatataaaa aacttctagc
     9721 taaaactaga agtttaaagg atcttatccg ctctgtccac tgtaaagagg gccacagtca
     9781 tcaggatata gatgagcaag agggcagcta cagctggtac ccaagagtgg aacaggtcaa
     9841 aactgtaacc aaagagggtt ggtccaaagg ctgctaggat atagcctcct gtttgagata
     9901 ggccggacaa ttgggctgtc ttttcagggg cgcttgtctt gagtgaaaag ttgaccatga
     9961 gataagggaa gagggcgctg gttgcggttc cgatgaggag atggatggta agccagtaaa
    10021 tgaaattatt gattgggaaa aagagcatgg aaatgccgac cacaccagct agtgaaacca
    10081 gagtgagcat gagctgacgg ttgcgagtag ataaactggt tgtcaggctt gggatggtca
    10141 ttgaaaaagg aatgctaatc agagataaga tagaagtcag caagccagct tcgtgactgg
    10201 atagacctgc atggatagac atggtaggta accaggtcat gacggtgtaa aagatcaagg
    10261 attgaaaacc tgaaaagata ataattgccc aaacctgttt attacgcatg acctttattt
    10321 gacttttttg tttggtttgt ggagctagtc tatgattata gcggtgattt gggagccaga
    10381 ccaaaaaagt tgctagacag agtaacgtga ggagaaggat aagtcctttc caagaactgg
    10441 cttgtgtaat gggcacagct agataggaag ccagagccgt tgcaatcccc atagacgtta
    10501 catataaggt ggtcagaaaa ccaattttct ttggttgatt ggcttggata agactaggaa
    10561 gcaggacatt gatgactgcg acacttgccc caaccatcaa ggttcctaga tagagtaggg
    10621 gcagattgat tagtcgaata agtgagccga tggtcaagaa gaagaggctg taggtgaaga
    10681 gatgctccaa gccgattttc tgagccagtt gggtagaaaa tggtgaaaag agggtaaaca
    10741 taaggagagg caggctggtc aagacaccaa gcgaactaac ttctacctct agaccttgcg
    10801 aaatatttcc caaaataatg ggtaaaacag taaaaggagt tcgcaaggaa acaccaatca
    10861 ggataatacc tggaacaaaa aagagtgatt gctttttcat ataatacctc ttatagctga
    10921 ttttaataac agcttatttt aaggcttttt cactataatg tcaagtaagg tttctggctt
    10981 tcaagataaa aatgggaaag tcttgaaaat tatgatagaa tggtggaagg aaaaattcag
    11041 gagagtagta gtgactcaaa atgttgaaag tcttctcgta tccattgtaa tcagtgcata
    11101 caatgaagaa aaatatctgc ctggtctaat tgaagactta aaaaatcaaa cctatcctaa
    11161 agagaatatt gaaattctat ttataaatgc tatgtccaca gatgggacca cagctatcat
    11221 tcagcaattt ataaaggaag atacagagtt taactcaatt agattgtata acaatcctaa
    11281 gaaaaatcaa gctagtggtt ttaacctggg agttaaacat tctgtagggg accttatttt
    11341 aaaaattgat gctcattcaa aagttactga gagttttgta atgaacaatg tggctattat
    11401 tcaacaaggt gaatttgtct gtggggggcc tagaccgacg attgtcgaag gaaaaggaaa
    11461 atgggcagag accttgcatc ttgttgagga aaatatgttt ggcagtagca ttgccaatta
    11521 tcgaaatagt tccgaggata gatatgtttc ttctattttt catgggatgt ataaacgaga
    11581 ggttttccag aaggttggtt tagtaaatga gcaacttggc cgaactgaag ataatgatat
    11641 tcattataga attcgagaac atggttataa aatccgctat agcccaagta ttctatctta
    11701 tcagtatatt cgaccaacat tcaagaaaat gctgcatcaa aagtattcaa atggtttgtg
    11761 gattggcttg acaagtcatg ttcagtttaa gtgtttatca ttatttcact atgttccttg
    11821 tttatttgtt ttgagtcttg tgtttagtct agcattgtta ccggtcacat tcgtattcat
    11881 aactttacta ttaggtgcct attttctact tttgtcatta ctcactttgc tgactttatt
    11941 aaaacataaa aatggatttc taattgtgat gccctttctt ttattttcca ttcactttgc
    12001 ttatggcctt gggacgattg taggtttaat tagaggattt aaatggaaga aggagtacaa
    12061 gagaacaata atttatttgg ataaaataag ccaaataaat caaaatatgc tacaataaca
    12121 atatagtaaa actcttttaa ggaggagtag atttctatga ataaaaaact aacagattat
    12181 gtgattgatc tggtggaaat tttaaataaa caacaaaagc aggttttctg gggaatattt
    12241 gatattttca gtatggtggt ttccataatt gtatcttata ttttatttta tgggctgatt
    12301 aatccagcac ctgttgacta cattatctat acgagtttgg ccttcctgtt ctatcaattg
    12361 atgattggtt tttgggggtt gaacgcgagc attagtcgtt acagcaagat tacggatttc
    12421 atgaaaatct tttttggtgt gactgctagc agtgtcttgt catatagtat ctgttatgcc
    12481 ttcttgccac tcttctccat ccgttttatc attctcttta tcttgttgag taccttcttg
    12541 attttattgc cacggattac ttggcagtta atctactcca gacgcaaaaa aggtagtggt
    12601 gatggagaac accgtcggac cttcttgatt ggtgccggtg atggtggggc tctctttatg
    12661 gatagttacc aacatccaac cagtgaatta gaactggtcg gtattttgga taaggattct
    12721 aagaaaaagg gtcaaaaact tggtggtatt cctgttttgg gctcttatga caatctgcct
    12781 gaattagcca aacgccatca aatcgagcgt gtcatcgttg cgattccgtc gctggatccg
    12841 tcagaatatg agcgtatctt gcagatgtgt aataagctgg gtgtcaaatg ttacaagatg
    12901 cctaaggttg aaactgttgt tcagggcctt caccaagcag gtactggctt ccaaaaaatt
    12961 gatattacgg accttttggg tcgtcaggaa atccgtcttg acgaatcgcg tctgggtgca
    13021 gaactgacag gtaagaccat cttagtcaca ggagctggag gttcaatcgg ttctgaaatc
    13081 tgtcgtcaag ttagtcgctt caatcctgaa cgcattgtct tgctcggtca tggggaaaac
    13141 tcaatctacc ttgtttatca tgaattgatt cgtaagttcc aagggattga ttatgtacct
    13201 gtgattgcgg acattcaaga ctatgatcgt ttgttgcaag tctttgaaca gtacaaacct
    13261 gctattgttt atcatgcggc agcccacaag catgttccta tgatggagcg caatccaaaa
    13321 gaagccttca aaaacaatat ccgtggaact tacaatgttg ctaaggctgt tgatgaagct
    13381 aaagtgtcta agatggttat gatttcgaca gataaggcag tcaatccacc aaatgttatg
    13441 ggagcaacca agcgcgtggc ggagttgatt gtcactggct ttaaccaacg tagccaatca
    13501 acctactgtg cagttcgttt tgggaatgtt cttggtagcc gtggtagtgt cattccagtc
    13561 tttgaacgtc agattgctga aggtgggcct gtaacggtga cagacttccg catgacccgt
    13621 tactttatga ccattccaga agctagccgt ctggttatcc atgctggtgc ttatgccaaa
    13681 gatggggaag tctttatcct tgatatgggc aaaccagtca agatttatga cttggccaag
    13741 aagatggtgc ttctaagtgg ccacactgaa agtgaaattc caatcgttga agttggaatc
    13801 cgcccaggtg aaaaactcta cgaagaactc ttggtatcaa ccgaactcgt tgacaatcaa
    13861 gttatggata agattttcgt tggtaaggtt aatgtcatgc ctttagaatc catcaatcaa
    13921 aagattggag agttccgcac tctcagtgga gatgagttga agcaagctat tatcgccttt
    13981 gctaatcaaa caacccacat tgaataaaaa agaaaaacgc atattatcaa gttaaacaac
    14041 cttggtaata tgcgttttat tatgtagaga cttatactct tcgaaaatct cttcaaatca
    14101 cgtcaacgtc gccttgccgt atatggttac tgacttcgtc agttctatcc acaacctcaa
    14161 aacagtgttt tgagctgact tcgtcagttc tatccacaac ctcaaaacag tgttttgagc
    14221 tgacttcgtc agttccatct acaaccttaa aacagtgttt tgagctgact tcgtcagttc
    14281 tatctgcaac ctcaaaacag tgttttgagc tgacttcgtc agttccatcc acaaccttaa
    14341 aacagtgttt tgagctgact tcgtcagttc catctacaat cttaaaacag tgttttgagc
    14401 agcccgcagc tagtttccta gtttgctctt tgattttcat tgagtattac ttcattttct
    14461 tctgaaatgg agttgttacc cagtctatgc tattgaaaat acgccaaaac ttctaagggt
    14521 ttgtgagcga tataatcagg ttgatagttt agtagatctg cttgctctcc aaatccccaa
    14581 gtgacggcca atttctgaat acctgtttct cgagctccca gcatatcaaa cttggtatct
    14641 ccgatgatga tggcttgttc tggtgctagt tgatgtgtct gcaaggcttg gtgaatgaca
    14701 tctgccttat ggggtgcttc agggctagaa ccataaatac catcaaagaa atgatggatt
    14761 tccaagtttt ttgccatgtc ttgagcagta gatgtattct ttgtcgtggt gatgtagagt
    14821 ggataactgc tcgataactc ctcaagcaag tctataatct gaggaaagag ttgagcttca
    14881 tagatgcctt ttgccttata gtaagaacga tatatctgca tggcttcaga aatttggtct
    14941 ttggacaggc aggtcgcaaa actactttcg agaggtggtc ccataaaacc acgaatagtt
    15001 ttggcatcag ggctaggcac ccccagctct ttaaaggtat aggtaaaggc attgtgaatc
    15061 ccgatagaac tatcaacgag ggttccatcc aaatcgaaaa aaatcgctgt gatagaggtc
    15121 atggtttctc ctatttgata agcttattct ccgaaaattt ctttttggag gcgacgacca
    15181 gtaggggtgg tagcgagtcc accttcagct gtttcacgaa aggcagttgg catgcttgct
    15241 cctacttggt acatggcatc gatcacttca tccacaggga ttttagattc gatacctgcc
    15301 aaggccatgt ctgctgcgat gaaagcaaag ctagctccca tggcattacg tttgacacag
    15361 ggaacttcga ccaaacctgc aacagggtca cagatgaggc ctagcatatt tttaatgaca
    15421 aaggcaatag cttgactggc ctgataaggt gttccacctg cagccagagt caaggcggca
    15481 gcactcatag cagaggctga accaacttca gcttgacacc caccctcagc acctgagatg
    15541 gaggcattgt ttgcgatgac tagtccaaag gcaccagcag caaagaggaa atccaattgt
    15601 tgctcgtggc tgaggtctaa tttttcaata gcagcagtga gaacggatgg cagacagcca
    15661 gcacttccag cggttggagt ggcacagacc aagcccattt tggcattgtg ttcattgact
    15721 gcgatggcat ttcgggcagc cgagagaatc gtataatctg acagagtttt tccgttttcg
    15781 atgtagtgat ccaatttggc agcatctcca cctgtcaggc cactacgaga tttattttca
    15841 ttgaggccaa gttggacaga ggctttcata acttccagat tgcgttccat gagaaggaag
    15901 acttcttcac gttcgcgacc ggtcaattca aactctgttg taatcatgag ttctgcgaca
    15961 tttccttgaa agtccagatc tgcttgctcg accaattctt tgatagaata aaacatgctt
    16021 cctcctattt aaagaaattg acattgtgga gatgagggat ttttcgaatt tcttcgatag
    16081 cctcatcaca gttgcgactg tcaacttcga taatcataat ggctttttca ccagcttttt
    16141 cacgagtgac attcatctgg gcgatattga taccatagcg ggaaagcgcc tctgtaacaa
    16201 gggcaatcat acctggaata tcttgatgaa cgatgatgat agtcggtgta ttcatattga
    16261 gagagacggc aaaaccattg agttcggtta cctgaatatt tcctccaccg atagaaatac
    16321 cagtcacgct gatggtcttg tgggcatttt taacagtaat tttagtggtg ttagggtgag
    16381 gggcattgct gtctttctga atggtccaga caatcttgat accacgcttg tgggcaattt
    16441 ccagactatt tggaatttca ggatcatctg tatccattcc taaaatacct gcaacaaggg
    16501 ctaggtctgt tccgtgacca cgataggtct tggcaaatga gttaaaaagt tggaattcaa
    16561 cttctgtcgg agtatcatca aaaatggaag agacaatctt cccaatacga acagcaccag
    16621 cggtatggct actagatggg ccaatcataa ctggtccgat gatatcaaag acagattgaa
    16681 aacgaagtga tttcatcagt ttccccttat aaaaattctt atctctatta tatcaaagaa
    16741 tgaggggctt ggctttaatt gtggatgaaa acctttctaa tacctcaaat agcataaaaa
    16801 tagtatcttt tatgacaaaa aacaccttat ttagggaaat aaaaaataat tttgtaatat
    16861 ttctacataa aagtgtcaag aaacggtaat atttaaaggg tatgatagaa ctatagaaag
    16921 aaggagaatt ttcgaatatg aaatcaataa ctaaaaagat taaagcaact cttgcaggag
    16981 tagctgcctt gtttgcagta tttgctccat catttgtatc tgctcaagaa tcatcaactt
    17041 acactgttaa agaaggtgat acactttcag aaatcgctga aactcacaac acaacagttg
    17101 aaaaattggc agaaaacaac cacattgata acattcattt gatttatgtt gatcaagagt
    17161 tggttatcga tggccctgta gcgcctgttg caacaccagc gccagctact tatgcggcac
    17221 cagccgctca agatgaaact gtttcagctc cagtggcaga aactccagta gtaagtgaaa
    17281 cagttgtttc aactgtaagc ggatctgaag cagaagccaa agaatggatc gctcaaaaag
    17341 aatcaggtgg tagctataca gctacaaatg gacgttatat cggacgttac caattaacag
    17401 attcatacct gaacggtgac tactcagctg aaaaccaaga acgtgtagca gatgcctacg
    17461 ttgcaggacg ttacggttca tggacagctg ctaaaaactt ctggcttaac aatggctggt
    17521 attaagaact aacgaacaaa taatatgtaa agagtcgagg aaatccctcg gcttttttat
    17581 ctttccttcg cttggtaata gatctctact tcttttttga gatgagacag catagaagtt
    17641 tcctcggtta gctctaggag tgagaaacct ttttggataa gtttcgcatc atccctacaa
    17701 atcccaatag ggacatagca gatagccagc ctatcgtagc ttttcttatt cttggcgtcc
    17761 tccagtaaag tgtagcagat ttgcttacac atgtttttat cttgattgta tagatagagt
    17821 gtggaaaggt tgagcaggat tgctattcgc aagtcatata aatgttgata gtttttatag
    17881 acttctaagc gttgcaagat tttctcggat tattgacaaa taaacggaca ttctgataga
    17941 cgattgccct agcggcttat tgtaaacttt gcaaatttgg cttgtaattt tttttataaa
    18001 attcacttcg attacttgac agttattata ttttcactta ttatatagat aaaaggatgg
    18061 gataagagtt taaaagtttg aaagcgctat ccatattgtt tattgggctg attcttgagt
    18121 aatagttaga gtgtgattaa tactactctg gttgttcaaa gacctgatac agaagagaac
    18181 tattctccta gttaagagta agttcgttgt ttaaaaatga aatgagatag aaggaaggtg
    18241 agtccaatgg actacgatac ttgttgacaa attactatag ctgtttctac ggaatagctc
    18301 tcgataatag gagaaataaa tcatgagaaa attagtgttg catctttaat ggtatgtagt
    18361 atacttgctt ctacaagtgt tttggcagta tgggttgatg gtggtcaatg gaactatgga
    18421 gtaggatggt caggaaattt tggttattct gattatttac attctactcg ctctcataca
    18481 gcaactgtta aagatggaaa taaattctct aaggatcgtg cagaagctga ggcatgggct
    18541 agagcttccc tcaccaagat tccgccaaca ggaatggaat atttctatgg atttgagtaa
    18601 aattcttccc tctcctattt ggagagggaa aactgtagtg gaggcacttt tatgaaaaaa
    18661 atcagtaatt tctgtatgtt actcctgctt ctgtgtacca ctttttttgt ttttaatgta
    18721 aactatacac gagaagtggt tcggattcaa gaaatgggaa agactgtaga ttctttggat
    18781 ttgtatttga aagatattaa cgaacctgca gcgtctgttc ttcgattttt tgaggatgta
    18841 tcaaaggagt ataaagtctc catcatcaaa acagacagtg gtgatgaggt ggtcaagtct
    18901 ggtgtttttg ataaagatac cttcccctac caagagtttg ggatttcttc tcttgatttt
    18961 accacagatg gtgaaggagt ctatagtaat aaagaaattt ccaataaact tggtacgatt
    19021 ccgacctttc taaaagccaa acctattcag cttatgactt ttcaaaccta tatcaaggat
    19081 acatctcgta gtttaaatgg tcgctatacg ataacttcta cacaagagat ggacaaggat
    19141 aggattgtac agaaatggag cgattttttc aagatagacc aggctacctt gctagagccg
    19201 acctacaaaa gtgcagtgga agtcataaat cgagatttgc ttttatctgc cattgttttt
    19261 gtcttggcta ttttgcttct tgtgttagtg acagtgtatc aaccgatgat ggagatgaaa
    19321 agagttgggg tacaaaaatt acttggtttt caagataggg ctgttttagc tgatgttgta
    19381 aaaggcaacc tttacctcct cctaggtggg gctcttgtga tcaatctagg cgtgtttttc
    19441 ttgcttgatt ataagccaaa agatttgttt cctatgctgt ggttgtctca ttttttgctg
    19501 ttgcagcttt atctctttat cagttggttg acttacctct taatccaaaa aatgacaatc
    19561 agctctctgc tgaaaggttt ttcatctttc aaatttggtc ttatcttcaa ttatgtgatg
    19621 aaaataggga caactatttt actgacggcc ttactgattg gggtgggcag aagtttagaa
    19681 caagaaaaca aagaacttgc ttatcagcaa cagtgggtaa gtcaaggtaa ttacctgacc
    19741 ttagaaacct tcaaactcaa tgataatctg tggcaagaag agctagcagg gtcagggaaa
    19801 tctacagatt atttctatcg attttatcag gatttggtag aaaaaacgca ggcgggctat
    19861 gtgcagagta gcagtcttcc tgtaaaaaat tttgtccaat cagaacagat tcagcaatat
    19921 cagttaacag atacggtgga tgtttactat gccaatcgca attttctaaa gagcaaggga
    19981 ttcaagctac cagataccgg tattaaaaaa gttattttga tgccagcaag tacgaaaggt
    20041 gaagaagata aaaatcagct cttggggaag ttaattgcct ttcattcgat gaagtatgaa
    20101 gagcagcaaa aacgaacgat agaggagatg gatgtcgaga ttgcctatta tgaaggagat
    20161 tggtcatttt tcccatatag tgataagcga aaggaaaatc tctccaatcc aattattagc
    20221 ttggtcaatg attctgatat gatgtgggat gagaaagcct ccctgtcaac aactggctta
    20281 aataatccga ttaaaattga aaatacggtt caacatcaaa aagagattac agagttagtt
    20341 gagaaattgt cagatggaaa ttatttaaaa ttttcatcta ttcaagccat tcaacaagag
    20401 aaagtggatt cttatagaga tgctgttcgg aattttaacc tactctttgc tttgtttggt
    20461 ctccttagca tgatgatttc ctacttctta ctagtaacaa ctttcttatt gaagcgcagg
    20521 gatatcatta ccaagaagtt tatggggtgg aaactggtcg atcgctaccg tcctctcctc
    20581 gttctgctct tgctgggcta tagtttccct cttctagtct tgattttctt tgcccatgcg
    20641 ttcttaccac ttctactgtt tgcaggtttt acatgtctgg atatactatt tgtgctaggc
    20701 ttagcttcta ggatggagaa aagaagtcta gtagagttat tgaaaggggg catcttatga
    20761 ttgagttgaa aaatattacc aaaaccattg ggggaaaagt gattttggat aacttatctc
    20821 tcaggattga tcagggggat ttggtagcta ttgttggtaa gagtggtagt gggaagtcga
    20881 ccttgttaaa tttattgggt ttgatagatg gtgattatag cggacggtat gagatttttg
    20941 gtcagacaaa tctagcggtt aattctgcta agtcgcaaac aataatccgt gaacatatct
    21001 cttatctgtt tcaaaatttt gccctgattg atgatgaaac ggtcgagtac aatctcatgc
    21061 tggcgctgaa atatgtgaaa ttgcctaaga aagacaagct caaaaaggtg gaagagattt
    21121 tagagagagt aggtttgtca gctactttgc atcaaagggt ctccgagttg tctgggggcg
    21181 aacaacaacg aattgcagtt gctagagcca tcttaaaacc cagccagctg attttagccg
    21241 atgaacctac aggttcgctg gatcctgaaa atagagattt ggtcttgaag tttctcttag
    21301 agatgaatcg agaagggaaa acagtcatta ttgtgaccca cgatgcttat gtagcccaac
    21361 aatgtcatcg tgtcattgaa ttgggcgagg gaaaatgagt tcattcagct ccttttgact
    21421 ggctgaatac tcatgttttc cagagaaaaa tggcataaat acgcctagga atgacatttt
    21481 atgtagtatt tctaggtttt tttgtttcaa attgaaaatt ttttcaattt aggcttgaca
    21541 aaggatgagt ataggagtat tatttataca ataaaaaaga ataaacataa aggaggcttt
    21601 gttatgaata agatgaagaa ggtgttgatg acgatgtttg gtttagtgat gctcccccta
    21661 ctatttgctt gtagtaacaa tcaatcggct ggaattgaag ccatcaagtc caaaggaaaa
    21721 ttggttgtag ccctcaatcc agattttgct ccatttgaat atcaaaaagt ggttgatggg
    21781 aaaaatcaga ttgtgggttc agatatcgaa ttagccaagg ctatcgcaac agaactaggt
    21841 gtcgaattgg aactatctcc tatgagtttt gacaatgtac tggctagcgt tcaatcagga
    21901 aaagctgacc ttgccatatc aggtgtttct aagacagatg aacggagcaa ggtgtttgat
    21961 ttttcaactc cctactatac tgcaaaaaat aaactcattg tcaaaaaatc tgatttagcc
    22021 acttatcagt ctgtcaacga tttggcgcag aaaaaggtcg gagcgcagaa aggttcgatt
    22081 caagagacga tggcgaaaga tttgctacaa aattcttccc tcgtatctct gcctaaaaat
    22141 gggaatttaa tcacagattt aaaatcaggg caagtggatg ccgttatttt tgaagaaccc
    22201 gttgccaagg gatttgtgga aaataatcct gatttagcaa tcgcagacct caattttgaa
    22261 aaagagcaag atgattccta tgcggtagcc atgaaaaaag atagcaagga attgaaagag
    22321 gcagtcgata aaaccattca aaagttgaag gagtctgggg aattagacaa actcattgag
    22381 gatgccttta aagcgtccat tgaaaaatag aaagaaggaa aagaacatga gtaaagaaaa
    22441 agtaattttg gcctattctg gcggtttaga cacatcagtt gctattacct ggctgaagaa
    22501 agactatgat gttgttgcag tttgtatgga tgtgggtgaa gggaaagact tagatttcat
    22561 ccatgataag gctcttaagg ttggggcagt cgaatcttat gtcattgatg ttaaggacga
    22621 atttgctaca gattatgtgc tagtagctct ccagtcacat gcctactatg aacaaaagta
    22681 ccccttggta tctgccttga gccgccctct tatttctaaa aaattggttg aaatagcgca
    22741 tcagacagga gcgactacaa ttgctcatgg ttgtacaggt aaggggaatg accaagtcga
    22801 ataccagatt gcagtcgcta agaaagcgaa tgaagctaaa aagtgatgga gtgatgggtt
    22861 cagaataaga gggtggtcaa ttggcagcct ctttcttgtc gttgaaaaag tgagatatat
    22921 tgacttttga aaaaaatgtc ataattgagt tgggataaac gtaaaagtca tactttctcg
    22981 ttaaattgtt cggcgattca tggtgacatg gccacatagt agtaaacaca ctatgtggct
    23041 tggcagagct aaaaacttct ggtttaacaa tggctggtat taagaactaa cgaacaaata
    23101 atatgtaaag agtcgaggaa atccctcggc ttttttatct ttccttcgct tggtaataga
    23161 tctctacttc ttttttgaga tgagacagca tagaagtttc ctcggtcagc tctaggagtg
    23221 agaacccttt ttggataagt ttcgcattat ccctacaaat cccaatgcgg acatagcaga
    23281 tagccagcct atcgtagctt ttcttattct tggcgtcctc cagtaaagtg tagcagattt
    23341 gcttacacat gtttttatct tgattgtata gatagagtgt ggaaaggttt aatagaattg
    23401 tcattcgcaa gtcatataaa tgttgatagt ttttatagac ttctaagcgt tgcaagattt
    23461 tctcggatta ttgacaaata aacggacatt ctgatagacg attgctctag cggcttagtg
    23521 taaactttac aaatttggct tgtaattttt ttataaaatt cactttagtt acttgacagt
    23581 tattatattt tcgctcataa tatagataaa aggatgggat aggagtttaa aaatttgaaa
    23641 gcgctatcca taatcgtaac gcataatttt ctccttttcg tgaaaaaagt taacgtaatt
    23701 ttgaaatgag cttaataaga ggtactatgt aggtagaaaa taaagatagg agaaaaagta
    23761 tgaataaaaa aatattttgt attctagtat gtattatatc attgattagt ctagctatag
    23821 tctttccatg gggctggtcg atataatgta agggagcttg agttgttagt ggaggataaa
    23881 atgttaagag taatagtagt tattcaatct ttagtaataa tactgcttgc tgttgcttta
    23941 ttattatctc ggaaaaactt tgattcgtta aatgttgttc ttataatgat gatcgtatca
    24001 caattgttgt tattagtata tttgataaaa tcagggaatc agtgattagc tgttatttga
    24061 attaagtaaa ctgttaagct tcaagcagta actggtttta aatatttaaa ataagataaa
    24121 ggaaggaagt ctaatttagg taactaaaca aagcctgcga tcacttgtta ggaaaccatc
    24181 ctaagcatgc ttaataaaaa ggagaaaaca aatggaactt gtattaccaa ataattatgt
    24241 tgtgattgat gaagaagaga tgatgtacct tgatgggggg gctatatata tacccaggtg
    24301 ggcaattaca ggagccatta ctggtgcagc atatgcagca ttagcagcag caggaggtgg
    24361 aggccttcaa ctagttcttg catcttatgg attacgctcc gcactggtag ctgggattgt
    24421 taaaggttta ggagtattag gaattcatat tggaaatgct tttgcaaata ctgttattag
    24481 aagtattgca tctgctggaa ttggtgctgg agctgattgg atttttacca atattattga
    24541 tggctgggat gggcgacgtg ataatcaatt gagaataggt taataatctt atgttgattg
    24601 ttttaatttc agttattgtt gcattcctgt atcataaaat tacagagtat gtcaacaaca
    24661 ggaaaattga tattgttatt tttctatcat tgctgatttc tcattttatt tggaattact
    24721 attttttacc aattgaaata ttgatagcaa tcagtggagc aaatttacta ttaagttttt
    24781 taaaaaaatt acagactaaa taatattaat ctgtgtaata cagtagctcg gtcattagat
    24841 tctattgaag atataaatta aaaatgaggc tgggaaaaaa ctcaatttga aaatgagatt
    24901 gagtaaatct ttctatctat ataacgtata tcatcaatat ttttgaacac ctgataatat
    24961 gcatttttct tattttaaag attttttgcc tttcttcatt tcttgaatga attggagaga
    25021 tgactttatt taggtgtttg acattactat agataggatt tagcagtact aatatgaact
    25081 catattatag gtgcagactt aaattttgga agcaagctaa aggatatctt atctaggtaa
    25141 ctaagtgcta taacaagata tattggtgga gttagtatgg caactattac taatgctctt
    25201 aatattgcgg ctactgttgc tgaagtcttc tcattaggag gagccattgc atatggttta
    25261 gatatcgtag acggtaaatt tgatgggtat ttatgggcgt aaaactatag ctatgaaaca
    25321 ttttttttgc aggaattggt gaaattcatt ttttgttatt tttattatat ctaagcatgt
    25381 ctgctagtcc aattattgga ctagtgattg gatatactaa aataaatcta ataaattcta
    25441 ttattatagc tcttggtatg atattggcat taattttggt attatcaaga gtgtatagaa
    25501 aatattttta tagagacgat gatgactaaa gtttttatca ataatttggg ctccttgtca
    25561 actgtagtgg gttgaataaa agctaacgtc tggggaaggg tttaaagctt tgctttatca
    25621 aatattgact tacagagttg ctctattgta ttgatgtatt cttagtaaat aatatggtgt
    25681 tttaaatgaa agatgatcag aaatatttat tagcgggact ttattcgctt ctagttgcaa
    25741 tattttattt tccattgatt gagtccaagg ggatttttgt aagtatattg atggcggttt
    25801 tacttttata tttaatatac ttcattgcaa cagtcattca tattgtgata attaagttta
    25861 taagaaaaaa atcttttaag tatttggttc tatatccatt tacttacgat gggagctgga
    25921 gatttcaacc tataaactta ctctactttc cagaaatggt gagagatgtc attccgatta
    25981 atttagttca agaatattgc caaggacaac cttacggact gttaaaaaag atgctgaaaa
    26041 gaattcgatt gtcacgagag atatctttac tcctggctac cattatcgtc tattttttta
    26101 cgcatagaat tctacctctt tcagtattta cgttcatatt ttcttacata ttgctatttg
    26161 cacaatctta tttgggtggt aataccgctt ggattggaaa tagaagatta ataatagatg
    26221 atgaatttga aaaaatcttg ctttcaaaaa gttatataaa agaaatatca tctgcacgat
    26281 actcagaata cttgacatgt gaatacaaaa actttacacc aatcatattg ttagctattt
    26341 ttgaaaattt gcttgactcc tacttgcttc agaatcagag tgaggttgac ttagatattt
    26401 tttataaagt tttacctttg ctttataaag aaaaatatac tatgggattc aattactttg
    26461 taagtttaaa ttatttacta tataaggttg gatttttggg aatcatttac gataatgaag
    26521 cattaagaga tttgtcaaaa caatatttaa acaaaaatat ttcggaatta caagatggtt
    26581 ctttcgaggg cggtatacaa gatgctgttg cctctaaaca aatagtagtt ataaatgaat
    26641 ttatagcttg tttaaattcc agatgcatgc catcccaata tgatagattt ttttataaag
    26701 atagacctta tattttttcc agaaagagtc ctattaaggg gtgaaaattt gttacatttg
    26761 gattaacaag tgtcctaggg attagaaata actctagtat agctagatat ttttgagtaa
    26821 ctagaaatat cattaggcta ctcatatgat tgccagaaaa gatggctgat gaatcttatt
    26881 tatactgttg cagtaagcat tgtctaggat aaataagcta tcttgatggg ggagattgga
    26941 ataattttag tagaaacatt aaaggattat gggcaagtag cgcaggatta ggtgatgcaa
    27001 tgtgctaagt tggcttggtg ttagcagtag tattatttaa caatggaaag tagactaaat
    27061 gaaaaataaa cgttattttt ttgacacaat tttaatgatt ttactgctta taagcacgat
    27121 attttgtgtc agcccagtat ttataaaatt ggacatctta ggaactccct ctcatgccat
    27181 cttaaccttt gtattggcga taccgttatt ctatattttg tctcaatgct tacatacttt
    27241 gctattacta gtttcctcaa tcttttgtaa attaagacca atttattttt attttatatt
    27301 tgtgatcatt ataggagcta ggaaatacta tcgaattctc tttcaccaac taatgggatt
    27361 tagtccagga gtagcagtgt tttataagga gagtcaaaca actaagaatc tgtttaaatt
    27421 ttactacttc ttgtacttta caacactaat ttcatactat tttttcttta cttttgtgta
    27481 tgacaaaccg ttgcttttgc ctcttattcc attttctatt atcatagcat tagtacaaaa
    27541 actttataga attgagaatc agcaattatt tctgttaaaa tcaaaggtac taacaatact
    27601 ggaatctaag aagaattgtg agtttaatct tcaggattat catgaaattt ggaaattgca
    27661 atcaaaatct gagttgccat gcgtggcttt atcttatatc agtttaatca agccatatct
    27721 ttcagagagt gtgcgtgaac aaattgattt attagaagtt aagcgattta aaaaaataaa
    27781 tcatccaata tcactttatg gtatgttgga tgttataaaa ttaaatctat atttacggca
    27841 ttataatgaa aaaaataaat atgaatcaat gttaaagaaa attttagagg taagaccaga
    27901 ctttgtttta atcgagcaga atattgatga tagtttaaat agctcgcaac cattatcttt
    27961 atctttagcc atttcagaaa tccaactttt gcttgaagtg tatatgggga tcaaacatgt
    28021 ttcaattagg agataaaacc agaataatat ttgccattat aggggcaagt tgcatattag
    28081 tcagtatgta tcttattcca cttattaaaa ctgagaataa attttgggaa atacttttat
    28141 ttatattagg tgtattgcta attggaattg cgttactaag tcaaaaaaaa taaattttga
    28201 aatagtattg gtgagttcgc aatcatttat tgctagtggt ttagaactgt taagtttcaa
    28261 acaataattg attttaaacc ttaggtaacc tataaaaaca aataaggtag agaaaggata
    28321 tcttatctag gtagctaagt gataaaaaca cagtcgtcta tcacttgtta ggaaactatc
    28381 ctaactatgc ttaataaaaa aggagaaaat aaatggaact cgtattacca aataattatg
    28441 ttgctcttga gcaagaagag atgatgtatc ttgatggggg attttctatt ctgagatggc
    28501 ctgttgcaac agccattaat atagctttta atggtgtttt aggtggagga gcaatcagtc
    28561 tagttagaaa ttatattcgt aattatggtt tgcggcgagt tacaagcgca attgctggag
    28621 cagctgcaag atatgttggg gtacgagttg caaatagagt ggcaggattt gcactgtctg
    28681 ctattaatgg atttgcagct tggatgtcaa ttggcgatgc tattacaaca atctgggcca
    28741 acaatgatgt aaataggaga gacccaaatt taaacgcctt gtggtaacta gatatgggta
    28801 ttaaaatgga aaaaattttt gttattattt tttttgtatg tctatttata tcatccataa
    28861 cttttttagc ctatgatttt gttagcgaag aaataaaaaa attgattatt tggatgaatg
    28921 tcgttttttt gattttaatc atagcaatga taatttatcc aaaattaaga aaatgataag
    28981 gacactgtaa aattatgata cgtaaactaa tcattttttt attaatgctc cctctttttg
    29041 tcttgtggct agagttgcat ttgttagtga gtaacttgca attgaatcta gaaattcccc
    29101 ttgattttgt ggttagtacg agtcttgctt tctgtagttt gattttatca aaaatagttt
    29161 tagacatact atacgcattg aaagatttgt acaagaaaga agctcttatt acgatttttc
    29221 cttttatttt tataggcagg aagaaagtaa atgtgaggtt ctcgccctat ttttcattcc
    29281 atcgtaaaag tttgtcgcct gatgatttaa gatctaggat tatatggagt tttattcttg
    29341 aaattgctat tattctagta tttattttaa aaattccttt tgcaatcatc atgcttacta
    29401 caatattttt ctggactata atggatatca atcatctcgt ttttaataag acggaatttc
    29461 tttttaatca aaataaatgg gaaaaagaag attcgtttga aagcgatttg acgaaaacgt
    29521 taaaggacaa aatacaaaaa tcagagttaa gctattctga cttaatgtcc cttcttctct
    29581 atgatgcgat gaaccaatcc acctttttaa cggatagtga actttttgag gatattttga
    29641 aaaaaatcga agattctcat aacacccttc tgtgtacagg tcttgtcgag ttactgctgt
    29701 atgagatttc tattagtaac aataacaact ggtccaacaa ggttgataaa attagaatac
    29761 agctcatcag aatcaatcaa ttagattttt tctattatac aagttggtta aggcaaaatt
    29821 ttgatttctg tatgaataga gagtatcata aaatgaaatc tcggaaatta ttactgagta
    29881 ataaaaagat agtttaagtt cgtcctattt gtaaaacaaa tgaaacaatt tcaactaagg
    29941 aggagaaaac aaatggaact tgtattacca aataattatg ttgtgattga tgaagaagta
    30001 atgatgtatc ttgatggggg agcttattta agcaagcgtg cttgtcaagg aatttgcgca
    30061 gcagcaggtg tattggctac agcagcaggg aaaatagctt actgtattgg atatggtgtt
    30121 cttaatagag gttgtgatat taacgggaac ccttatcctt gggatggatt catatctgca
    30181 acagtaagat gagtaatgta gataagattc gaaaaataca catcatcgtg tgttgggtgt
    30241 atatattttt atcattcagg gcgattataa atgatacgga gtattttttg cttatttttt
    30301 tagcttttat ctactcaata gtatcactac ctctatattc tgtgaaaaat aaaatagtat
    30361 ctatctgtct agccataaat tcgattctgt taatcagttt ccccatttta atcaataagt
    30421 ttttcccaga aagttttttg acttataccg tattaataag tgtttttatc ctagagttag
    30481 caatctttca tttaattggg gaagattttg ctactaaatt gactaacgaa tataagaaaa
    30541 ttagtcagtt tagaagcaag gtgtctcaat ctccttggat aaaatattta gagatttcta
    30601 gttttatatt aactatattt ccatctattc tttacggtac agttgataat cgtgtattgg
    30661 ctcttatctt tttgataaaa atttgtgtag atactacgat aaaattttta ttcatcagat
    30721 tatttgacac aagtacttta atgaagagga gaatattttt tcttttcgca ttggatgtta
    30781 tagcttactt atttttagga tatcttttag tgattcaaaa agcaggctat ttgttttcgg
    30841 ttttacttct tttttctaat ttttcagttc catttatcaa agcaaaggaa tacgaattat
    30901 ttaaaaatag caagtaatag aaacataggt taaatcttag aacggcttaa aatcagatat
    30961 gataaaggga aatagtctta tctaggtaac taagtgctac aaacacagtc gaggatcact
    31021 tgttaggatt acattttaat catgcttaat aagaatggag aattcctatg gggattgtat
    31081 tactctgaag tttcgaatac cttagttctc gtgattttga gctgttctag ctttagaatc
    31141 cttcagaatg taaaatttta agcagtcaaa gacttagaat agcctggaaa catttatttt
    31201 atagcaattc taggcttaga attgctcata tatatgtatt tgagctgctc gatagttagg
    31261 acagctctat tctgagaaat ctgagctatc aatgcttcag aatagtattt cgcaaatggt
    31321 ttttattgaa gaggtgattg ttttatgata gtctaaaata aaactgattt aatggagtaa
    31381 atggagaatc gtcacatgaa acattttttt gcaggaattg gtgagataaa acttgttggc
    31441 tacttattgt atatatgtgt aggactagcc cctctttttc atatatatgt cataggttcg
    31501 gaactgagtt ttgtgaaaat agtgttatct attttgggag ttatatttgt aagtatgtta
    31561 actattgctc gaatttatcg gacttttcta taaagaatag tttggacttg aactgtatct
    31621 aaaaacagag gttggtcgat tatcagcctc tttcttgtcg ttgaaaagag tgagatatat
    31681 tgacttttga aaaaaatgtc ataattgagt tgggataaac ctaaaagtaa tatagtagat
    31741 tgaatctgga atagtccgtt atgactgtta aaatatttct agaaattaat ttgactttcc
    31801 taatcgattt gttcacatct tgtttcaatc tactatactt tctcgttaaa ccgttcggca
    31861 attcatggtg acatggacac atagtagtaa gcacactatg tggcttggca gagctaaaaa
    31921 ctgttccctt gcgataagcc taataagcac aacataggga gttagagaag ccgactctaa
    31981 tcaaccactt tgggcagtag tgagaactgc cccgtgcttt tttattttga gaaaatatgg
    32041 agtttgtcgt tgaaattact tgattgtatt ttggactatc aagaaagatt caatggaaaa
    32101 acatgtcaag tatcaacgaa ttataagtat ttagagattt tcaaagtgaa tttttgcttg
    32161 actgatttac atcatttatt tgacttatac aaaatcacac gagattatgc tagtcaaaca
    32221 aaacctgcta ttcaagctgg tgtttttatt ttagaagatt ttagaaatat cctatgtaca
    32281 atgatgtaat cgaaaggata tctttatatg agtttatcgg tgatattttc tattctaaaa
    32341 taataagttg ttgcatcgta gctagggatt tatctaaaaa tactatgaaa ttggacgtca
    32401 tattttttga ggataaaaat aaaagatccg cagttttagg tttacgaaga gacaaaagcg
    32461 gagtatttaa atcagttact ctacatttta caagcgctaa gaaatatgct aaagttcgta
    32521 aaacagatgt gaaagaaatg aaatggctat aaagattgtc cgcaggctta agcttgcgtt
    32581 ttttgttgtt taacgaaatt aaaaaacaag ctctctcatc ggaaatgttt ttataggaat
    32641 gaaggaagat gatgctatgt ttaaaaagag aatcgaaaag ggcaaaagta gtgtctttat
    32701 ctttttggag tagaagctta tgatatagaa atttgtaata aaaatgtaat ataaaacatt
    32761 tgacaaatat ttacgggggg agtatactta atataagtat agtctaaaaa tgactatcag
    32821 aaaagaggta aatttagatg aataagaaaa aaatgatttt aacaagtcta gccagcgtcg
    32881 ctatcttagg ggctggtttt gttacgtctc agcctacttt tgtaagagca gaagaagctc
    32941 cacaagttgt cgaaaaatct tcattagaga agaaatatga ggaagcaaaa gcaaaagctg
    33001 atactgccaa gaaagattac gaaacggcta aaaagaaagc agaagacgct cagaagaaat
    33061 atgatgagga tcagaagaaa actgaggata aggcaaaagc ggttaagaaa gttgatgaag
    33121 aacttcaaaa agcaaattta gaacttcaaa aagctcatgt aaaatatcaa aaagcacaaa
    33181 gagaacttag tgaaagtcca gacagaagaa aatctgctgc tagaaagaaa ttagctgatg
    33241 cattatctta tattggagag gtagagttaa aacaaaaaga ggcggaagcg aattttaata
    33301 cggaacaagc aaaagtaatt cccaaagaaa ctgagttagc tgttactaaa caaaaagcgg
    33361 aagaagctaa aaaagaagca gaagtagcta agaaaaaatt tgataaggca gctcaagagg
    33421 tagaagtagc gaagaaagaa gtagaggcta aggaacttga aattgaaaaa cttcaagatg
    33481 aaatttctac tttggaacaa gaagttgcta ctgctcaaca tcaagtagac aatttgaaaa
    33541 aacttcttgc tggtgtggat cctgatgata cagaagctat agaagctaaa ttaaaaaaag
    33601 gggaagctga gctaaacgct aaacaagctg agttagcaaa aaaacaaaca gaacttgaaa
    33661 aacttcttga cagccttgat cctgaaggta agactcagga tgaattagat aaagaagctg
    33721 ctgaagctga gttggataaa aaagctgatg aacttcaaaa taaagttgct gatttagaaa
    33781 aagaaattag taaccttgaa atattacttg gaggggctga tcctgaagat gatactgctg
    33841 ctcttcaaaa taaattagct actaaaaaag ctgaatttga aaaaactcaa aaagaattag
    33901 atgcagctct taatgagtta ggccctgatg gagatgaaga agaaactcca gcgcc
//
LOCUS       DBERDK010000015        33919 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.015, whole genome shotgun sequence.
ACCESSION   DBERDK010000015 DBERDK010000000
VERSION     DBERDK010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 33919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33919
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.015"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(1..1136)
                     /locus_tag="ACL73L_000708"
                     /pseudo
     CDS             complement(1..1136)
                     /locus_tag="ACL73L_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285359.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1118..1425
                     /locus_tag="ACL73L_000709"
                     /pseudo
     CDS             1118..1425
                     /locus_tag="ACL73L_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1580..1903)
                     /gene="tnpB"
                     /locus_tag="ACL73L_000710"
     CDS             complement(1580..1903)
                     /gene="tnpB"
                     /locus_tag="ACL73L_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGS0771723.1"
                     /translation="MTIHLSSLGQVYLVCGKTGMRQGIDSLAYLVKTHFELNSFSGQV
                     FLFCGGRKDRFKVLYWDGQGFWLLYKRFENGRLTWPSTEKDVKALAPEQVDWLMKGFS
                     ITPKI"
     gene            complement(1884..2069)
                     /locus_tag="ACL73L_000711"
     CDS             complement(1884..2069)
                     /locus_tag="ACL73L_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771724.1"
                     /translation="MPQPIVPVEIPQSRRFDSKKRNDILLKIRIGKLEVSFFQSLNLE
                     MIEQLLDKVLLYDNSSI"
     gene            complement(2373..3935)
                     /gene="guaA"
                     /locus_tag="ACL73L_000712"
     CDS             complement(2373..3935)
                     /gene="guaA"
                     /locus_tag="ACL73L_000712"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HGS0771725.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHK
                     ISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
                     CPYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKADWSMDNFIDMQIKK
                     IRETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHI
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            4077..4775
                     /locus_tag="ACL73L_000713"
     CDS             4077..4775
                     /locus_tag="ACL73L_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGS0771726.1"
                     /translation="MLPAYMKIHDQIKKDIDEHRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERRVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPEKFIKDFKKEEITSHFF
                     QTLQKYGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQVSYLEDGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(4818..5714)
                     /locus_tag="ACL73L_000714"
     CDS             complement(4818..5714)
                     /locus_tag="ACL73L_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGS0771727.1"
                     /translation="MSNSLKGTLLTVVAGIAWGLSGTSGQYLMAHGISALVLTNLRLL
                     IAGGILMLLAYATAKDKILVFLKDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTA
                     TVLQYVCPVGILIYSCIKDRVAPTLGEIVSIIFAIGGTFLIATHGQLDQLSMTPAGLF
                     WGLFSALTYALYIILPIALIKKWGSSLVIGVGMVIAGLVALPFTGVLQADIPTSLDFL
                     LAFAGIILIGTVFAYTAFLKGASLIGPVKSSLLASIEPISAVFFAFLIMNEQFYPIDF
                     IGMAMILFAVTLISLKDLFLEK"
     gene            complement(5723..6658)
                     /locus_tag="ACL73L_000715"
     CDS             complement(5723..6658)
                     /locus_tag="ACL73L_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HGS0771728.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYLGQSDPEH
                     GLQTEAGLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTAPELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKFGPVELAVPTVRGVEAGIVHDPKARLLGRHTGSAGLFSTI
                     KDLQIFLEHYLADDFARDLNQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(6655..7380)
                     /locus_tag="ACL73L_000716"
     CDS             complement(6655..7380)
                     /locus_tag="ACL73L_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HGS0771729.1"
                     /translation="MNVNQIVRIIPTLKANNRKLNETFYIETLGMKALLEESAFLSLG
                     DQTGLEKLVLEEAPSMRTRKVEGRKKLARLIVKVENPLEIEGILSKTDSIHRLYKGQN
                     GYAFEIFSPEDDLILIHAEDDRASLVEVGEKPEFQTDLASISLSKFEISMELHLPTDI
                     ESFLESSEIGASLDFIPAQGQDLTVDNTVTWDLSMLKFLVNELDIASLRQKFESTEYF
                     IPKSEKFFLGKDRNNVELWFEEV"
     gene            complement(7464..8099)
                     /locus_tag="ACL73L_000717"
     CDS             complement(7464..8099)
                     /locus_tag="ACL73L_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HGS0771730.1"
                     /translation="MAVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKTIVNRGIRGYQTGLLLENLDAHLYGGAVDKIFLLIGTNDIGKDVPVN
                     EALNSLEAIIQSVARDYPLTEIKLLSILPVNEGEKYQQAVYIRSNEKIQNWNQAYQEL
                     ASAYMQVEFVPVFDCLTDQAGQLKKEYTTDGLHLSIAGYQALSKALKDYLY"
     gene            complement(8101..8928)
                     /locus_tag="ACL73L_000718"
     CDS             complement(8101..8928)
                     /locus_tag="ACL73L_000718"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HGS0771731.1"
                     /translation="MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQAQTWDDAIVNKALAHFKGRACQ
                     DQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINALFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEI
                     MAFGDSENDVEMLEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(8950..9285)
                     /locus_tag="ACL73L_000719"
     CDS             complement(8950..9285)
                     /locus_tag="ACL73L_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HGS0771732.1"
                     /translation="MGNFISNQRIESMGDEENAKWTERGVLMDVTIKKKDGKTTIGTA
                     KAHPTWVNRTPKGTFSPEGYPLYHYQTYILEDFIEDGSHRDQLDEATKERIDTAYKEM
                     NEHVGLKWY"
     gene            complement(9286..9876)
                     /locus_tag="ACL73L_000720"
     CDS             complement(9286..9876)
                     /locus_tag="ACL73L_000720"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HGS0771733.1"
                     /translation="MGDFEGTINKDHYLAGYLLFNAPAEVMDAIKEAGYHVLDLAHNH
                     ILDSQIEGVISTADIIEKAGITPIGVYTHEPRDQALLVIKEVNGIKVALLAYSYGFNG
                     IEQYISQEDYNRYLSDLNEDKMKVEIERAEKEADITIIMLQMGVEYRLELTEEQKALY
                     HKMIDWGADIIFGGHPHVVEPSETVEKDGDKKLIIY"
     gene            complement(9892..10014)
                     /locus_tag="ACL73L_000721"
     CDS             complement(9892..10014)
                     /locus_tag="ACL73L_000721"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771734.1"
                     /translation="MAHGDLLYHDGLFFSAKKEDGTYDFHENFEYVTPWLKQGD"
     gene            10527..11138
                     /gene="def"
                     /locus_tag="ACL73L_000722"
     CDS             10527..11138
                     /gene="def"
                     /locus_tag="ACL73L_000722"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HGS0771735.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRAIAEEVTFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEA
                     YDLEAIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHR
                     IKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            11183..11911
                     /gene="rlmB"
                     /locus_tag="ACL73L_000723"
     CDS             11183..11911
                     /gene="rlmB"
                     /locus_tag="ACL73L_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HGS0771736.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELAT
                     EKKVSISWTSKKSLSEMTEGAVHQGFVLRVSEFAYSELDYILAKTRQEENPLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRAVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFRNRL"
     gene            complement(11950..12861)
                     /gene="trxB"
                     /locus_tag="ACL73L_000724"
     CDS             complement(11950..12861)
                     /gene="trxB"
                     /locus_tag="ACL73L_000724"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HGS0771737.1"
                     /translation="MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYETRT
                     VIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEALFLT
                     RFAKTVTIVHRRDQLRAQKVLQDRAFANEKISFIWDSVVREIKGENRVESVVFENVKT
                     GQVTEQAFGGVFIYVGLDPLSDFVKELNIQDQAGWIVTDSHMKTAVDGIFAVGDVRLK
                     DLRQVTTAVGDGAIAGQEAYKFITEHS"
     gene            complement(12927..13151)
                     /locus_tag="ACL73L_000725"
     CDS             complement(12927..13151)
                     /locus_tag="ACL73L_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HGS0771738.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTAKARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(13229..13972)
                     /locus_tag="ACL73L_000726"
     CDS             complement(13229..13972)
                     /locus_tag="ACL73L_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGS0771739.1"
                     /translation="MIKISNLSKSFSGQTVLDHLDLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKSGQTMILVSHDMSFVAQVADKILFLDKGKII
                     ESGTPDEIIHTPKEERTKEFFASYKRTYI"
     gene            complement(13972..14772)
                     /locus_tag="ACL73L_000727"
     CDS             complement(13972..14772)
                     /locus_tag="ACL73L_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGS0771740.1"
                     /translation="MNVTTILASDWYQNLMQLIPDGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNPGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSAGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMAISAPDTVQTDVKGDLR"
     gene            complement(14930..15286)
                     /locus_tag="ACL73L_000728"
     CDS             complement(14930..15286)
                     /locus_tag="ACL73L_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HGS0771741.1"
                     /translation="MLEFIEYPKCSTCKKAKQELNQLGVDYKAVHIVEETPSQEVILN
                     WLETSGFELKQFFNTSGIKYRELGLKDKVGSLSNQEAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(15299..15811)
                     /locus_tag="ACL73L_000729"
     CDS             complement(15299..15811)
                     /locus_tag="ACL73L_000729"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001170346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HGS0771742.1"
                     /translation="MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGKPQDLSNLLLAPIGTNFEKRVWDYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
                     EGVDFKDINK"
     gene            complement(15811..16305)
                     /locus_tag="ACL73L_000730"
     CDS             complement(15811..16305)
                     /locus_tag="ACL73L_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HGS0771743.1"
                     /translation="MEIRLAFPNEVGAIMQVIEDAKKCLADAGSDQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSPEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKVMQHIFEKLGFKQVGKVPVDGERLAYQ
                     KLKK"
     gene            16440..16886
                     /locus_tag="ACL73L_000731"
     CDS             16440..16886
                     /locus_tag="ACL73L_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HGS0771744.1"
                     /translation="MKTTFSYPKWAEIPNINLYLDQVLLYVNQVCAPISPNKDKGLTA
                     SMVNNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQTLNTLQTQASSDQ
                     LYDAFVDYMNQGIDPANPIIQTSCQTVKLYHQTLDLIDHTQEEVIQ"
     gene            16883..17530
                     /locus_tag="ACL73L_000732"
     CDS             16883..17530
                     /locus_tag="ACL73L_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HGS0771745.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKKVNEKFSLALYLIMGWLVLAIIPAII
                     SQTTPVFWSLMVTGGLCYTVGAGFYAKKKPYFHMIWHLFILAASALQYIAIVYYM"
     gene            complement(17551..18132)
                     /gene="pdxT"
                     /locus_tag="ACL73L_000733"
     CDS             complement(17551..18132)
                     /gene="pdxT"
                     /locus_tag="ACL73L_000733"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HGS0771746.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESHLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEGVEILATVNNQIVA
                     AQEKNMLVSSFHPELTDDVRLHQYFINMCKEKS"
     gene            complement(18133..19008)
                     /gene="pdxS"
                     /locus_tag="ACL73L_000734"
     CDS             complement(18133..19008)
                     /gene="pdxS"
                     /locus_tag="ACL73L_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042819 - vitamin B6 biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HGS0771747.1"
                     /translation="MTENRYELNKNLAQMLKGGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLREDELYVAAKDLQVPVELVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFRNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            complement(19160..20539)
                     /gene="nox"
                     /locus_tag="ACL73L_000735"
     CDS             complement(19160..20539)
                     /gene="nox"
                     /locus_tag="ACL73L_000735"
                     /EC_number="1.6.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H2O-forming NADH oxidase"
                     /protein_id="HGS0771748.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKEKLEAKGAKVYMNSPVLSIDYDNKVVTAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALADGKIELFRNGAF
                     LVDKKQETSIPGVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDLQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAEK"
     gene            20833..21756
                     /locus_tag="ACL73L_000736"
     CDS             20833..21756
                     /locus_tag="ACL73L_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HGS0771749.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGIAPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     SDAKVAQPQEIESVLPLINPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVT
                     SGIYERHLTVDGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     ILWQVESLEGIEVILIDKEGHLSCSSGIPTL"
     gene            21816..22421
                     /locus_tag="ACL73L_000737"
     CDS             21816..22421
                     /locus_tag="ACL73L_000737"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HGS0771750.1"
                     /translation="MLKLIAIVGTNSKRSTNRQLLQYMQKHFADKAEIELVEIKAIPV
                     FNKPADKQVPAEILEIAAKIEEADGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLSHSLQAFNPSGDLVDLD
                     VIKKLDAIFDDFRIFVKITEKLRNAQELLRKDAEDFDWENL"
     gene            22440..23684
                     /locus_tag="ACL73L_000738"
     CDS             22440..23684
                     /locus_tag="ACL73L_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HGS0771751.1"
                     /translation="MKFVGLVGSNYDQSYNRKLLEFIRRHFKLKFELEVLEIDEVPMF
                     NQDEKWDESFQLRYLYNKITRADGVIIATPEHNHTISASLKSVLEWLSYEVHPFENKP
                     VMIVGASYYDQGTSRAQVHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDNNGNITNEG
                     TVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPIATTITEVDPDDPEWVEKVA
                     AITGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYADDNNQFLYYNNAHQDPDTMF
                     AKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQEYVRTIVPGSPAGVINTHN
                     YQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGGSGPFAPAAGGHGDADATSG
                     ASDSGDAGGHGGDADATAGASY"
     gene            complement(23801..24058)
                     /locus_tag="ACL73L_000739"
     CDS             complement(23801..24058)
                     /locus_tag="ACL73L_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HGS0771752.1"
                     /translation="MDPKKIARINELAKKKKTEGLTLEEKVEQAKLREEYIEGYRRAV
                     RHHIEGIKIVDEEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            complement(24100..26136)
                     /gene="glyS"
                     /locus_tag="ACL73L_000740"
     CDS             complement(24100..26136)
                     /gene="glyS"
                     /locus_tag="ACL73L_000740"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HGS0771753.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKGKRLSFEAI
                     QTFSTPRRLAVRVTGLSDKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYVTKEEVGQAVEAIVPGVVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEQEFDLDFLDIKGSRVSRGHRFLGKETKIQSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKEIEAKHGVRIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKAGLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILDAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEDFKPSVESLSRAFNLAEKAEGGATVDSALFENDQEKALAEAVETLVLSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            complement(26348..27265)
                     /gene="glyQ"
                     /locus_tag="ACL73L_000741"
     CDS             complement(26348..27265)
                     /gene="glyQ"
                     /locus_tag="ACL73L_000741"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HGS0771754.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATSPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEISDQEMLLENFD
                     KFEKEAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKRLGYPLLDEETRAKLLAEDAE"
     gene            complement(27634..28211)
                     /locus_tag="ACL73L_000742"
                     /pseudo
     CDS             complement(27634..28211)
                     /locus_tag="ACL73L_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397459.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
     gene            complement(28484..29326)
                     /locus_tag="ACL73L_000743"
     CDS             complement(28484..29326)
                     /locus_tag="ACL73L_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HGS0771755.1"
                     /translation="MNTYQLNNGVEIPVLGFGTFKAKDGEEAYHAVLEALKAGYRHID
                     TAAIYQNEESVGQAIKDSGVPREEMFVTTKLWNSQQTYEQTRQALEKSIEKLGLDYLD
                     LYLIHWPNPKPLRENDAWKTRNAEVWRAMEDLYQEGKIRAIGVSNFLPHHLDALLETA
                     TIVPAVNQVRLAPGVYQDQVVAYCREKGILLEAWGPFGQGELFDSKQVQEIAANHGKS
                     IAQIALAWSLAEGFLPLPKSVTTSRIQANLDCFGIELSHEERETLKTIAVQSGAPRVD
                     DVDF"
     gene            complement(29440..30831)
                     /gene="pgdA"
                     /locus_tag="ACL73L_000744"
     CDS             complement(29440..30831)
                     /gene="pgdA"
                     /locus_tag="ACL73L_000744"
                     /EC_number="3.5.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050119 - N-acetylglucosamine deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-N-acetylglucosamine deacetylase
                     PgdA"
                     /protein_id="HGS0771756.1"
                     /translation="MDKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIELLKKEKDDQLSEGNQKEYFRQGQAEVIAYYPLQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKIEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDASKAKEQLIKELTSFIEDKKIEQDQSEQIVKSFSDQDLSAWNFDYKDS
                     QIILYPSPVLENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKYQKVVALTFDD
                     GPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEASILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
     gene            complement(31069..31437)
                     /locus_tag="ACL73L_000745"
                     /pseudo
     CDS             complement(31069..31437)
                     /locus_tag="ACL73L_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000603324.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
     gene            31658..32635
                     /locus_tag="ACL73L_000746"
     CDS             31658..32635
                     /locus_tag="ACL73L_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HGS0771757.1"
                     /translation="MLKLGVIGTGAISHHFIEAAHTSGEYQLVAIYSRKLETAATFAS
                     RYQNIQLFDQLEVFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVTQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLADKQVLGADFNYAKYSSKMPDLLAG
                     QTPNVFSDRFAGGALMDLGIYPLYAAVRLFEKANDATYHAQQLDNSIDLNGDGILFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFTDHQGNQVQIPIQQAPHT
                     MTEEVAAFAHMIQQPDLNLYQTWLYDAGSVHELLYTMRQTAGIRFEAEK"
     gene            32632..33714
                     /locus_tag="ACL73L_000747"
     CDS             32632..33714
                     /locus_tag="ACL73L_000747"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGS0771758.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTPIQTQLFEPLLAGENLLGVSQT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLVYMSATTKFDQEKIVPNTRTIDLSDQKLDNIQHFYMQVDQRHRVD
                     ILRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILAVSLASDVNVKFRKVILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYTHRAGRTGRMGKEGYVITLVTH
                     PEEIKKLKKFASIREIVLKNQELYIK"
     gene            complement(33778..>33919)
                     /locus_tag="ACL73L_000748"
     CDS             complement(33778..>33919)
                     /locus_tag="ACL73L_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HGS0771759.1"
                     /translation="RGFKNKAFRTLEDVMNQLQDVIQGLEKEVIKSIVNQRWTRMLFE
                     SR"
BASE COUNT        10032 a         7596 c         6132 g        10159 t
ORIGIN      
        1 ctctttgttt acgagagttt cctcgtttgg aagacattct agaagataga atagatattt
       61 ctctctattt aattgatgac gtttagctgt ttccaacaaa ctcataataa tagctcttgc
      121 tttagctcct tcaaaacttt gagaaaacaa ccaccctcgc ttttttaaac tgagcttagg
      181 ctaaaagagt ccagtggact cttttactcc gtcccataac caatgattta atggcgtgtt
      241 cagctagatt attggaaagg accagatgtc cgtcttttaa aatggtctta aaggtttctt
      301 catacttgag gctgtattca attgcccttc ctggttttga acctgctaaa actgactgac
      361 ggcggcacca agcaaagaag tcttccatta agggctggac gtttctgtag tcgttcatca
      421 gctggcaaag cctcccagtc tctttccaag gcaaataact ggtcacgata ggctaaacct
      481 ttagctccta aggatgattt atctgcttgc ttgggggtcg cttcaaaaaa tttccttctc
      541 acatgcgccc aacatccaac aagttcagct tcttccagtt gacgataagc ttctagcggt
      601 tcgcagttta ccaagcttag cgtcgccttg ctcctaaacc ttagactgct atcgcatagg
      661 cagaactaga ggactaaagt tctaagttac tgccgcaaca tatcacaatg catatagcca
      721 gaataatttc ctaggaattc ttgcactacc gaaccactcc gacgctgatc atggtggtac
      781 agcgtaatcc cttgtttctc agctttccca gacaaaaaag tccaatagta agtcagctga
      841 ctatcacttt ctaagacccg ataagaggtt tcatctgcat gaagaagagc ttgttctaac
      901 aacttttctc gtaaaaggtt ataaaggggc tccaaatagt attgactcgt cttgatatgc
      961 caattagaga tttccttacg tgtgattggt aaacccatcc tagcccaatc ttcttcttgg
     1021 cgataattgg gtaccttcag attaaacttc tgatggatgg tgtgagcgat aatagaagct
     1081 gagccaaggc tatgcgccaa aggggcttta ggaataggag ctttcacaag cttatccaga
     1141 tgattatctt ttactcgtta tgaacaatgc tatatggcat aaatcaagta ccttaaagat
     1201 tccgactaat attggctttg catttattcc tccatacaca ccagagatga acccattgaa
     1261 caagtgtgga aagagattcg taaacgtgga tttaagaata aagcctttca aactttggaa
     1321 gatgtcatga atcaacttca agatgttata caaggactgg agaaggaggt gataaagtcc
     1381 atcgttaatc ggagatggac tagaatgctt tttgaaagca gatgagtata aaaagaaagt
     1441 cctcatttca atagaaatca cgactttctg atggatttat agtaaaatga aataataaca
     1501 ggacaaatcg atcaggacag tcaaatcgat ttctaacaat gttttagaag caaaggtgta
     1561 ctattctagt ttcaatctac tatatttttg gagtgataga aaagcctttc atcagccaat
     1621 ctacttgttc aggtgcgaga gctttgacat ccttttctgt actgggccaa gtcagtctgc
     1681 cgttctcaaa gcgtttatat aatagccaaa atccttgacc atcccagtaa aggactttaa
     1741 agcggtcttt acgtccacca caaaagagaa aaacttgacc tgagaaagaa ttcaattcaa
     1801 agtgggtttt aaccagataa gccagtgaat cgattccttg tctcataccc gttttcccac
     1861 atacgagata gacctgccct aggctagata gatgaattgt catagagcaa caccttatcc
     1921 aaaagctgtt ctatcatttc gagattgaga gattgaaaaa aacttacttc aagcttgcca
     1981 atacgaattt taagcagaat atcatttctc tttttagaat caaaacgacg agattgtgga
     2041 atctctacag gaacaatagg ttgtggcatt aaaatatcct ccaatagttt tactttacta
     2101 atagtataca ggaggagctc catgaatgat agataccttg ttatgacgcg cttaccatat
     2161 tagaaagttg gctctctaat taaattaatt ctcattatat atatcaaaaa accgcagctt
     2221 gaaaactacg gtctaatttt ttgtccaaat gtaaaccact tggaactatt attcaattta
     2281 ttctttttag caaatgttcc cgttttaatc ccagttacag tttttaatcc tctgtaaccg
     2341 ttgattttac aaagctttta atttttttgc gattattccc actcaactgt tgcaggtggt
     2401 ttacttgtaa tatcgtagac gatacggtta acatgatcca cttcatttac gatacgtact
     2461 gagatttttt gaagtacttc ccatggaatt ttggcaaaat cagcagtcat accatcgata
     2521 gaagtgatag cacggattgc aatcgtgtag tcatacgtac gaccgtcacc cataacaccg
     2581 actgagcgaa cgcctgtgtt aacagtgaag tattgccaaa tatcgcggtc aagtccagct
     2641 ttagcgattt cttcacgaag aatagcgtct gattcacgaa cggtttcaag tttctcttca
     2701 gtgatttcac ccatgacacg gatagcaagt cctggtcctg ggaatggttg gcgccatacg
     2761 atgtggtctg gcataccaag ctctgtacca agagcacgaa cttcatcctt gtaaagagta
     2821 ttgagtggtt caatcagttc aaactgcata tcttctggaa gaccacccac gttgtggtgt
     2881 gacttgatag tttgagctgt atccgtacca gactcgataa catctgtata taaagtacct
     2941 tgagcaagga atttcacatc tttgagcttg cttgcttcgt catcgaatac atagacaaac
     3001 tcgttaccga tgattttacg tttttgttca ggatcagaaa cgccagcaag tttgtcaagg
     3061 aaacgtttag cagcgtctgc tttgacgata ttcaaaccaa acttaccacc gagcatgtcc
     3121 ataacttgat cagcttcgcc tttacgaaga agaccgtggt ctacgaagat acagatcaat
     3181 tgatcgccaa tcgctttttg gagaagaacc ccaacgacag atgagtcaac accacctgat
     3241 agaccaagaa ggacacgttt atcaccgacg gtttcacgaa tttttttgat ctgcatatca
     3301 atgaaattat ccattgacca gtcagcttta gccttacaaa tgttaagggc aaagttacga
     3361 aggatatcat ttccgtatac agaatgacga acttctgggt ggaattggat accgtaaatg
     3421 tgtttatctg ggttttcgat ggctgcgtat gggcagtcag ctgatgtacc tgtacgaaca
     3481 aagtcagcag gaatctcagt aaccgcatca ccatggctca tcaaaacagt ctgttcatca
     3541 ggtgttgatt caaaaagcgc tgatggtgtg tgagttaggg ttgattgacc gtattcacga
     3601 tttccagcat cacctgcagg aacaactttt cctccaagtt tatgggtcaa taactgcata
     3661 ccataacaga ttcccaaaat tggaattcct agttcgaaga tttctgggtc aatgtcaaat
     3721 gaaccatctt catatacaga atttggacca cctgatagaa taattcctac aggattgact
     3781 tcacgaactt cagcagctga aattttatgg ctttttagtt ctgaaaaaac accaatctca
     3841 cggatacggc gtgaaatcag ctggttgtac tggctaccat agtccaatac gatgattttt
     3901 tctacatctt gcaaatcagt tgaaatgttg ctcatctttt tcctttctca aaagaaatat
     3961 cttttctcaa tattctatca caaataaggg cgaattacca actaaacaag gcgaagtttg
     4021 actttttcta gtttattggg gtacaataga gaaaagatag aaatgaagtg aaaaatatgc
     4081 tacctgctta tatgaaaatc catgatcaga taaaaaagga tattgacgag caccgttggg
     4141 ctattggtga gaggcttccc agtgaaagag atttagctga gcagtttgcg gtcagtcgca
     4201 tgaccctccg ccaagccgta tctctattag tcgaagaagg cgtcttagag cgccgtgtag
     4261 gaagcggcac ctttgtttcc agtactcgag tacaagaaaa gatgcgaggg acaaccagtt
     4321 ttactgaaat tgtcaaatcc caaggtaaag ttccctctag ccagctcatt tcctacaaaa
     4381 aaaccattcc caatgagcaa gaagttgcca agctaggaat ttttccaacg gacaatatta
     4441 tccgaatgga gcgggtccgc tatgccgacc aagttcccct agtttatgaa gttgcttcta
     4501 ttcctgaaaa attcattaag gactttaaaa aagaagaaat caccagtcat ttcttccaaa
     4561 ccttgcaaaa atatggctat cgtatcggca aatctcaaca gaccatctat gctcgccttg
     4621 ctaaagaaaa gattgcccac tatttggaag ttgaaaaagg acatgctatt cttggattga
     4681 cacaggtttc ctacctagaa gatggtactg cttttgaata cgtaaaaagt caatatgtag
     4741 gcgaacgctt tgaattttat cttgaaaata attagattac cacgcaaaaa ctctctgtca
     4801 cagacaaaga gtctttttta tttttctaag aataaatctt tcaaagaaat caaagttaca
     4861 gcaaacaata tcattgccat accaataaaa tcaatgggat aaaattgttc attcattatt
     4921 aagaaggcaa agaaaaccgc cgatattggc tcaattgaag ccaacaagct tgacttgacc
     4981 ggtcctatca gactggctcc tttaaggaaa gctgtatagg caaagacagt cccgataagg
     5041 ataatgcctg caaacgcaag gagaaaatca agactagtcg ggatatcggc ctgtagaacc
     5101 cctgtaaaag gaagggcgac caaacctgct atgaccattc ccacaccaat gaccaagctg
     5161 ctcccccact ttttaatcaa ggctatgggt aaaatgatat acagagcata agtcaaggca
     5221 gaaaagagac cccagaacag gccagcaggt gtcatggata actggtccaa ctgcccatgt
     5281 gttgcgatca ggaaggttcc tccgatggcg aatatgatgg aaactatctc tcccagtgtc
     5341 ggtgccaccc tatccttgat acagctataa attaaaattc cgacaggaca aacatactga
     5401 agcaccgtcg ctgttcccgc attggtctcc tgaatagcag acagataggc gaattggttg
     5461 agaaaaagac caatcagagc aaaaataaga agagacagca aactctttct atcctttaaa
     5521 aagaccagta ttttatcctt tgcagtagca taagccaaga gcatgagaat tccaccagcg
     5581 attaaaagac gcaagttggt taagaccaga gccgaaattc cgtgtgccat caggtattgg
     5641 ccactcgttc ctgacaaccc ccaagcaata ccagccacaa ctgttagtaa agtccctttt
     5701 aaactatttg acatgtctct ccttactctt ctttgcgaat caagttcatc acttgattgc
     5761 ggtctaatat ccattgagct ctctcgtcct tttcataggt acgattcgat aggaaaatag
     5821 tggcttcttg cttctgacga ttccacatga taaaggtacc tgtatagccc gtatggtcta
     5881 gccaatctcc ttccaaattc catgctaaag aacgttcctt gtcatccaaa ggagaaaaat
     5941 tttgatttaa gtctcttgca aaatcatctg ctaaatagtg ttctaaaaag atttgtaaat
     6001 cctttatagt cgaaaataaa ccagcactcc cagtatgtct acccaggaga cgagccttgg
     6061 gatcatgcac tatgcctgcc tctacacctc taactgttgg aacagcaagc tcaactggcc
     6121 caaacttggt ttccgtcatt ccccaaggtt tccagacttg atcctttaaa atcacatcca
     6181 aatcttgatt aaaaattctt tccaaaataa agcccaacag caaaaaatgg acatccgaat
     6241 aaagaaaggc tggctgactt cgtctgttga gatgaaacat cgcttccttt aattcagggg
     6301 ctgttaaaag atcacgatta ggaataaaag gatcaaggtc tgttgcatga gtcaagagct
     6361 ggcgaatagt gatgtctgga taatcactct caggtaaaaa atctattacc agtctatcaa
     6421 tatctaattg acctatttcc cacaagaagg tacaaactgt gccaacccca acaaccttgc
     6481 tgacactagc taggtcataa actagtcctg cctcagtctg caagccatgc tctgggtcac
     6541 tctggcctaa atagaactct gtccattgat tgtccttaaa atacgcaaaa gaggctccgg
     6601 gataaattcc tgcctcgatt tgttcttcta tttttttaat aatcttggtc cacttcatac
     6661 ttcttcaaac cacaattcaa cattatttct atctttacca aggaagaatt tttcagactt
     6721 aggaataaaa tattcagtag actcaaactt ctggcgaaga cttgctatgt ctaattcatt
     6781 gaccaagaac ttgagcatag ataagtccca ggtaaccgta ttgtccacag tcaaatcctg
     6841 cccctgagct ggaataaaat caagggatgc cccaatttca gatgattcca agaaactttc
     6901 gatatcagtt gggagatgta attccataga aatctcaaat ttacttaaag aaattgatgc
     6961 caaatctgtt tgaaattcag gcttttctcc tacttctact agacttgctc tgtcatcttc
     7021 cgcatgaatc aaaatcaaat catcttctgg tgagaaaatt tcaaaagcgt agccattttg
     7081 acctttatat aatcgatgaa tcgaatctgt tttagataag attccttcaa tttctaaggg
     7141 attttccacc ttgacaatca atctagctag tttttttctt ccctctacct tacgagtacg
     7201 catactggga gcttcttcta aaaccagctt ttcaagaccc gtttggtcac ctagtgacag
     7261 aaaggccgat tcttctaaca aggccttcat tccaagggtt tcaatataaa atgtttcatt
     7321 taattttcta ttattagctt ttaaagtagg aataatccgt acaatctgat ttacattcat
     7381 aaattcctcc gtcacttttt attttaaagg attttagaat tttttacaag atattatgct
     7441 caaatttaac attatttagc tatttagtaa agatagtctt tcaaggcttt tgacaaagcc
     7501 tgataaccag caatactgag gtgcagtcca tcagttgtat attctttttt gagttggcct
     7561 gcttggtctg tcaaacaatc aaatactggc acaaattcca cctgcatata ggcagatgca
     7621 agctcttgat aggcttgatt ccaattctga attttttcat tcgagcggat atagactgcc
     7681 tgctggtact tctctccctc attgacaggc aaaatggaaa gcaatttaat ctctgtcaat
     7741 ggataatcgc gagcaacgga ttgaatgata gcttcgagac tattgagagc ctcattcaca
     7801 ggaacatcct ttccgatatc atttgtccca atcagaagaa aaattttatc tactgctcca
     7861 ccatatagat gagcatcaag gttctctagt aacagtcctg tctgatagcc acgaattcct
     7921 cgattgacaa tcgtctttga agtcccaaat agctcctgta gaggataata ctcgacaatg
     7981 gaatccccaa taaaaagaat gtttggttct acaacagaaa tgtgatttag gtgacgatat
     8041 ttggtttgaa ttttttcttg ttcctttagg agccaatttt ctaataactg tactgccact
     8101 tcattctcct ttttctaacc agttttccaa gacttgataa actcctcctt ggctgttggc
     8161 tggtgctaga gcagtcgcca cagctttggc tttctcatca gcattttcca tcgcataggc
     8221 aattccagcc atttcaagca tttcaacatc attttcacta tcaccaaaag ccatgatttc
     8281 ttgggatttc aagtcccagc gcttgagtaa ttcctccaag ccccatgctt tatgaatccc
     8341 agcttggagg atatcaatgc aaccatagcc actggataca gctcggacac ggccatcaaa
     8401 gagagcattg atttcttcca aaaccgaact caaacgttcc tcaccaacaa ccatgctcat
     8461 cttgagcaca ccaccaaaga ggtcagatgt taattcatcc acaaattgca tccgttggta
     8521 gaatttttca atcatttctg gagtcataaa actttcaaga tctgtaaaaa tcgtaccttc
     8581 cttgacaaaa tcacccttca tccccgttac aacaaactgg tcctgacacg ctcgaccctt
     8641 gaaatgagcc aaagccttgt tgacaatggc gtcatcccat gtctgagcct gaatcaattc
     8701 attgttttca aaaatacgag cgccattagc aacaaccaga accactcgat ccaccaaggg
     8761 actcagtagt tgtctcatgc ggtgaatttc attgcccgtc gcaatgacaa aacgaatgcc
     8821 cctttgatcc aactgatcta aaatcttttc caatcgtggg agatcaagct gacctctagc
     8881 atccagcaag gtcccatcca tatctgtcgc aattacctta atcatcattt actttcctct
     8941 ggattcaagc taataccact tcaatcccac atgttcattc atttctttat aggctgtatc
     9001 aattcgttcc ttagtcgctt catctaactg gtcacgatga ctgccatcct ctataaaatc
     9061 ttccaaaata taagtttggt aatgatacaa gggatatcct tctggtgaaa aggttccctt
     9121 tggtgttcga ttgacccaag taggatgagc tttagctgtt ccgatagttg tttttccatc
     9181 cttcttcttg atggtgacat ccatgagaac accacgttca gtccacttag cattctcttc
     9241 atctcccata gattcaatcc gttgattgga aatgaagttc cccatttaat agataatgag
     9301 tttcttatct ccatcttttt caaccgtttc agatggttca acaacgtgag gatgccctcc
     9361 aaagataata tccgctcccc aatcgatcat cttgtgataa agagcttttt gttcttcagt
     9421 tagttccaat cgatactcaa cacccatctg aagcatgata atggtgatat ctgcttcctt
     9481 ctctgcccgt tcaatttcaa ccttcatctt atcttcgttt aaatctgaaa gataacgatt
     9541 atagtcttcc tgagaaatat actgctcaat tccattgaaa ccataggaat aggctaacaa
     9601 tgcaaccttg ataccattca cttccttaat gaccagcaga gcctgatcac gtggttcgtg
     9661 cgtataaact ccgattggag tgattccagc tttctcaata atatcggccg ttgaaataac
     9721 tccctcaatt tgcgaatcca aaatatgatt atgagctaaa tccagcacat gataacctgc
     9781 ctccttaata gcatccataa cttcagcagg agcattaaag agaagataac ccgctaaata
     9841 atgatcctta ttaatggttc cttcaaaatc accaatagct aaatctgctg cttagtcccc
     9901 ttgcttgagc caaggagtca catactcaaa attttcatga aagtcatagg taccgtcttc
     9961 ttttttagct gaaaagaaaa gtccatcgtg gtagagcaaa tccccgtggg ccataattct
    10021 ggcagttttt tcctcttcct actcctgaga cttttgctta gtcccctctt gagaaatagt
    10081 atctcgtttt tgactagtca agggattcct tggaagcttt caaacaacaa gaccaagccc
    10141 attgataaat caactgctag caagagtatc gccacaaatc ccttattgct ccacttgcga
    10201 taactcctaa aaagtttacc aagcccttca taaaacgaaa agctaaacca ccctgatttc
    10261 gattttgtct tctttgtatc ttcattctcc ctactttctt atgcaagcct tttcttttta
    10321 ttatatcaca gataagtatt tctttcacaa ttgaattgaa cttcccatct attttctata
    10381 aatcctaaat gccataatgc tttcaattct tgtcattttg tgatatcatg tagaggaaat
    10441 gaactaatcc acagtggctt attccaagta taccacttgg gctttggcag tagctaactg
    10501 cactaaacat aatataagga gagaaaatgt ctgcaataga acgtattaca aaagctgctc
    10561 acttaattga tatgaacgat attatccgtg aagggaatcc tactctacgc gcgattgctg
    10621 aggaagtcac tttcccccta tctgaccagg aaatcatcct aggcgaaaag atgatgcaat
    10681 tccttaaaca ttcccaagat cctgtcatgg ctgaaaaaat gggactccgc ggtggtgttg
    10741 gactggctgc tccccagtta gatatctcaa aacgcattat cgctgttttg gtacctaata
    10801 ttgttgaaga aggcgaaact ccacaggaag cctacgattt ggaagccatt atgtacaatc
    10861 caaaaatcgt ctctcactct gttcaagatg ctgctcttgg cgaaggagaa ggttgcctgt
    10921 ctgttgaccg taacgtgcct ggctatgttg ttcgccatgc ccgcgttact gttgactact
    10981 ttgacaaaga tggagaaaaa caccgtatca aactcaaagg ctacaactcc attgttgttc
    11041 agcatgaaat tgaccacatt aacggtatca tgttttacga tcgcatcaat gaaaaagacc
    11101 catttgcagt taaagatggt ttactgattc ttgaataaag aaaatcccgt tgcaagacgg
    11161 ggttttgtgt tataatagag gcatgaaaac aaatgatatt gtctatggtg tccacgccgt
    11221 taccgaagct ctccttgcaa atacaggaaa caaactctac ctccaagaag atctccgagg
    11281 taagaatgtt gagaaagtca aggaactagc tacagaaaag aaggtgtcca tttcttggac
    11341 atcaaaaaaa tctctctctg agatgactga aggtgctgtt catcaaggtt ttgttctacg
    11401 agtgtctgaa tttgcctata gcgagctaga ttacatcctt gcaaaaacac gccaagaaga
    11461 aaatccactt ctattgattc tagatggtct aaccgatccc cataatctgg gttctatctt
    11521 gcgaacagcc gatgcgacca atgtttcagg tgtcatcatt cccaagcacc gtgctgtcgg
    11581 agtaactcct gtcgttgcca aaacagccac aggtgctatt gaacacgttc caattgcccg
    11641 agtgacaaac ctcagtcaaa ccttagataa actcaaggat gaaggcttct ggacctttgg
    11701 aacggatatg aacggtactc cttgccacaa gtggaataca aaagggaaaa tcgccctcat
    11761 cattggaaat gaaggaaaag gtatctctag caacatcaaa aaacaagtcg atgagatgat
    11821 taccattccg atgaatggac atgttcaaag ccttaatgcc agtgttgctg cggccattct
    11881 catgtacgaa gttttccgaa atagactata aaaaagtttc cagtcatctg attggaaact
    11941 tttttatgat taactatgtt ctgtgataaa cttgtaggct tcttgaccag cgatagctcc
    12001 atctccaact gctgttgtta cttggcgaag gtctttcaag cgaacatctc caactgcaaa
    12061 gataccgtcg actgcagtct tcatgtggct atctgtcaca atccagcctg cctgatcttg
    12121 gatattcaat tctttaacaa aatcgctaag agggtccaaa ccaacataga taaagacacc
    12181 accgaaggct tgttctgtca cttgacctgt tttcacattt tcaaatacga ctgattctac
    12241 tcggttttca cccttgattt cccttactac agaatcccag ataaagctga ttttttcatt
    12301 cgcaaaggcg cgatcttgta aaaccttttg ggcacgaagt tggtcacgac ggtgaacaat
    12361 ggtaacagtc ttagcaaaac gagtcaagaa gagggcttct tcaacagctg aatctccacc
    12421 accaactacc aataaatctt ggtcacggaa gaaagcacca tcacacacag cacagtaaga
    12481 aacaccacga ctgttcagtt cttcttctcc aggcactccc aaaggacggt gtttagaacc
    12541 agttgctacg ataactgtac gtgtttcata tgtttggtca tcagtcatca ctttcttaaa
    12601 atcaccatgg tcttcgacat tttcaacata accataaatg tgctcaacac caagattttc
    12661 aagtggttca aacatctttt cagccaattc aggtccacta atattagcgt atcctgggta
    12721 attttcgata tcagatgtat tattcatctg accacctggc agaccacctt caatcaaagc
    12781 tacttttaga ttgcttcgag cagcatacaa ggccgcagtc atccctgcag gtccagcacc
    12841 gataataata gtatcgtaca tatagattcc ttctttcttg ttgtaactat ctttattcta
    12901 actctttctt gtcaatcacg caaggattat gccttgaaaa caagaattaa actcataacc
    12961 gcaaaggata atactgggac aaccaagact ccaatcataa tcatatcata gagatgggct
    13021 tggcgagcct tggctgtctt atcaactccc gacatggctc tcagaccaat ccaaatccct
    13081 aaaaaaatca gaacaaggat ggtggtcaat atcaaactct cgaaatataa agaaaatagt
    13141 tgcagtagca tgatttctct catttctatc ttttttaaag agtaaactca gctagtccaa
    13201 ctaactgagt tttcctttat ctattatatc aaatataagt ccgtttgtaa ctagcgaaga
    13261 attcttttgt ccgctcttct ttaggggtgt ggataatctc atccggagtt ccagactcga
    13321 tgattttccc cttatctaag aagagaatct tatccgcaac ttgggctaca aaggacatgt
    13381 catgactgac caaaatcatg gtctgacctg acttagcagc atctgcaata gacttttcta
    13441 cttcaccgac caattctggg tcaagggctg aagttggttc gtctaagagc aaaacatctg
    13501 gtttcatagc aagcgcacgc gctagggcaa cccgttgctt ctgtccacct gataaatggc
    13561 gaggataatg gttttcacgg tccgaaagcc caaccttagc caactcttcc ttggcaatct
    13621 tagtcgcttc ttggtcagat aatttcttga caacaaccaa gccttctttc acattatcaa
    13681 gtgctgttcg gcgttcaaac aaattaaact gttggaaaac catagacaac ttacggcgta
    13741 gggcaaggat ttcttcttga gtgattttag aaaaatcaac tgaaaaacca tcaatctgaa
    13801 tagagccact gtcaggtgtt tcaagataat tgagactgcg aagaaaggtt gattttccag
    13861 ctcctgaaga accaatcaag gctacaactt cccctttttg aatatccaag tccagatgat
    13921 ccaagacagt ctgtcctgaa aaggatttgc ttaaattcga aatcttaatc attaacgaag
    13981 gtctcctttc acatctgttt gcactgtatc aggtgcagaa atagccattt ttctctcgat
    14041 gaaacgaccg aggctttcaa ttccgatatt gactacccaa taaacaaggg caacggagat
    14101 gaagcgttca aaatagcgat aatcagctcc acctagaatc tgagcttggg caaagacttc
    14161 cacaacaccc gcactaaaag ctagagatgt tcccttggtc aaaccgatga gggaattaat
    14221 caaggttgga gtagctacca ccgctgcatt aggaataatc actcgtcgat aaacttgcgc
    14281 tcgggtcata cccagactgc gtgccgcctc aatctcacca ggattaactg agagaatggc
    14341 tgcacgaatg gtttcactag cataagctgc ctcattaaag gcaaaagcga caatcgcaaa
    14401 agctgcagct ggaatcgcat tgatattgag accagttccc cattgctgat tgagggcttt
    14461 caaagccaaa gggattccgt agtaggtcaa catgagttgc accaaaatcg gtgtcccttt
    14521 taagaaacta acaaagaagg cctgcaaggg atataaaatc ttgacacgat tgatcttcac
    14581 aatggcaaaa agaagcgcca aaaccaagcc aaaaagggca ccaccaattg tcaacataat
    14641 tgttgttgga agttgttgga caattctagg gattccatca aagaccgaac gtaggctaaa
    14701 cagcttgcca tccggaatca attgcatcaa gttttggtac caatctgatg ctaaaatcgt
    14761 tgtaacattc ataaaaacat cctcttctga taatgattca ttctaccatg cttctgccgt
    14821 caatgaaatt atttgctacg gtcagataca gactttgtct acctactagt ttatcatact
    14881 ttgaaacagg gaagttatat attttatcta tcaaagagat agataaaaac tatttcagtc
    14941 ccagttcttc ataagatttt cgataaccga tttgcttaac agttccattt tctactaaaa
    15001 tgggccgttt taacaacata ccgtcacttg ctagcaactc agccgcttct tggtttgaca
    15061 aacttcctac cttatctttt agccctaatt cacggtattt gataccactg gtgttgaaaa
    15121 attgcttcaa ttcaaatcct gaggtttcta gccaattcaa aatgacttct tggctaggtg
    15181 tttcttccac gatatggacg gctttatagt ccacacctaa ttgatttaat tcttgttttg
    15241 cttttttaca agttgaacat ttggggtatt cgataaattc taacatgtgc ttctccttct
    15301 atttgtttat atctttaaaa tctacgcctt catgctccaa gagccaagct ttcttttcca
    15361 ctcctgcagc atagcctgtc agacgcttgc ctgctcccaa cacacgatga caaggtacta
    15421 ggatagacca aggattgcgt cccactgctc caccaattgc ttgagcagaa gccacttgca
    15481 ggtcttgagc aatttgtcca taggtcactg tctgaccata aggaatgccc tgtaaatagt
    15541 cccaaactct cttttcaaaa ttcgttccga ttggcgccaa gagcaagttg gataaatcct
    15601 gaggcttgcc tttaaagtaa tcatctaagc aagcaataac tgggtctaaa ataggatgac
    15661 taacaacttc ttctatcgtt tcatctccta gtcccctctc aaaatgcttc tgctcctgaa
    15721 cccaaattcc atacaaataa tggtcatcag ctacaagtga tagaatacca attggtgagg
    15781 agtagaggat ttttacatac ttcttttgca ttatttcttt aatttttgat aggccaagcg
    15841 ttcgccatct actggcacct taccaacttg tttaaaacca agtttttcaa aaatatgttg
    15901 cataaccttg ttttcagcat gcgtatctga gcgaaaatca agataatcaa aaccttcaat
    15961 caagccctct aagaaggttt gagcaactcc ttttccctgc acatctgctg ccacagcaat
    16021 acggtgaaag actagatact ctgactctcc agcttgccag tttccctcat aaatagcttc
    16081 ataggcctcc tctggactct tggtcacagc agcataggct agcagttctc cctcttccaa
    16141 ggctacgtag gcttgccctg agatgatatc atcgataata acatctgcat ttggatagcc
    16201 attttgccac tggtcactac cagcatctgc taaacatttt ttggcatcct ctatcacctg
    16261 catgatggca cctacttcat ttggaaaagc taaacgaatt tccatctttt ttcctcattt
    16321 ctgactagtt tctcccatca tagcataatt taagtctttc tacaagcagt taaaacttga
    16381 ctttgtattt ttgttatttt ataatctagt tatcaaaact atataaaaag gagttgaaaa
    16441 tgaaaactac cttttcctac ccaaaatggg cagaaattcc aaacattaac ctctatctgg
    16501 accaggtttt gctctatgtc aatcaggtct gcgcccctat ctctcctaat aaagacaaag
    16561 gcctaacagc atctatggtc aacaattatg tgaaaaatgg ttacctgaca aagcctgaca
    16621 aaaaaaaata ccaacgccaa cagattgccc gtttgattgc tatcacaacc ctcaagtctg
    16681 tattttctat tcaagaaata gcccagacac ttaatactct acaaactcaa gcaagttcag
    16741 accaactcta cgatgctttt gtggactaca tgaaccaagg aattgaccca gctaacccta
    16801 ttatccaaac cagctgccaa accgttaaac tctatcatca aactctagac ttaatcgatc
    16861 atactcaaga ggaggtaatc caatgaacac tagtcttaaa ctcagcaaac aactcagttt
    16921 tggagaggag attgctaata gcgtgaccca tgctgtgggt gcagtcatca tgcttatctt
    16981 gctgcctatt tcatccatct atagttatga agcacacgga tttttatcct ctatcggcgt
    17041 ttccattttc gtcatcagtc tctttctcat gttcctatca tccaccattt atcactctat
    17101 ggcctatggt tcgacccaca aatatgtttt gcgaatcatt gaccattcta tgatttacgt
    17161 tgccattgcc ggctcataca cgcccgttgt cttgaccttg atgaataact ggtttggcta
    17221 tctgattatt gtcatccaat ggggaacgac catctttggt attctctata aaatctttgc
    17281 taaaaaggtc aatgagaaat ttagccttgc tctttacctg attatgggct ggttggttct
    17341 ggctatcatt cctgccatta tcagtcaaac gacacccgtt ttctggagtc tcatggtaac
    17401 tggcggactc tgttatacag ttggagctgg attttatgcc aagaaaaaac cttatttcca
    17461 catgatttgg catctcttta tcctagctgc gtccgcactc caatacatcg ctattgttta
    17521 ttacatgtaa aaaagttgag aagttcaatc tcaacttttt tctttacaca tattgataaa
    17581 gtactggtgc aagcgcacat catcagtcaa ttctggatga aaagaactta ccaacatatt
    17641 tttttcttgg gctgcaacaa tttgattgtt cactgttgct agaatttcta caccctcacc
    17701 aacactactg ataatcggac cacggataaa ggtcattgga atcttgccaa ctcccttaca
    17761 ttctgcttcc gtgtagaaac ttcctaattg gcgcccataa gcattacgct cgaccaccat
    17821 atccatagtt cctagatgac tctctttctg agaagtgatt tccttagcca gcaaaattaa
    17881 gcccgcacag gtcccaaaca ctggtaagcc agatagaatg gcttctcgga tgggaagtag
    17941 catgttctgg tcacgtaaga gcttgcccat ggttgtagac tcaccaccag gcaaaatcaa
    18001 acccgacaag tcactctgat cttgctgaaa atcatctaga tttctgagtt ctacactctc
    18061 gacacctaat tgatctagca cttttgcatg ttctgcaaag gccccttgca aggccaatat
    18121 tccgattttc atctattttc ctcgttcagc catgagaatt tggatttcat tttcattaat
    18181 accaaccatg gcttctccta aatcttcaga gatttgagct aggatttgag gattacggaa
    18241 gttagtcaca gccttaacaa tggcactcgc tcgtttaaca ggatctcctg acttgaaaat
    18301 acctgaaccg acaaagaccc cctctgcccc taattgcatc attaacgcag catctgctgg
    18361 cgttgcaaca cctccagcag cgaaatttac aactggcaat tttccatgtt catgaacata
    18421 ttggaccaat tctacaggga cttgcaaatc cttggcagca acataaagct cgtcctcacg
    18481 taagttttga atgcggcgaa tttcctgatt catcatacgc atatgacgaa cagcttggac
    18541 gatatcccct gtccctggtt ctcctttggt acgaatcatg gaagcacctt cagcgatacg
    18601 acgcaaggct tcacccaaat ccttagcacc acagacaaaa ggaacttgga attctttctt
    18661 gtccacatgg aaacggtcat cagctggaga tagaacttca ctctcgtcga tataatcaat
    18721 ttcaatagcc tctaaaatct gagcttcaac aaaatgcccg attctgacct tagccattac
    18781 tggaatacta accgcttctt ggatttcctt aatcatcttt gggtcgctca tgcgggaaac
    18841 tcctccagct gcacgaatat cagccggaat tcgttccaag gccatcacag ctgccgcacc
    18901 agcagcttct gcgatacgag cctgttcagg attctgcaca tccataataa caccaccctt
    18961 gagcatctgt gccaagtttt tatttagttc ataacgattt tcagtcattt gttttatcct
    19021 catcatttgt attggtagct accttttcat tttaagtcag aacagaatga agcacaagat
    19081 aaaaaaacta taattgtatg gggacagatt ggctaaaaac tatctgacct taacttaagg
    19141 ccagatagct cattcatttc tatttttcag ccgtaagggc agccattgtg atgtagttgt
    19201 atggtttgtt gaagtgtggc aagaagaaga ggtctgtcaa tgccaattta tcaattgtca
    19261 catgctcttg gatagcaagt gagaacatgt ggattcccat gctaattgca atatcatgtg
    19321 aaaccatttg ggcaccaaga atttcacggc tatctttgtc aaagacaatc ttaattgcta
    19381 cttcatggtt gtcatgtttc atgaattctg gtttttgaag atcgttaaag cctgtttcag
    19441 ttgcgttgta accagcagct ttcgcttttt caagagtcaa accagttgaa accatgtgaa
    19501 gaccgtagat tgagatacca tttgaacctt gaacaccgat tccttccaat tcatgtccac
    19561 aagcattgta ggcaccaacg ataccagtgc gaacagcatt tgaagcaaga gcgatatagc
    19621 ttgtatcttt acgagcattg tcataaacag tcgcacagtc accaacagcg taaacgcctg
    19681 ggatagatgt ttcttgtttc ttgtctacaa ggaaggcacc gttgcggaag agttcgatct
    19741 taccatctgc aagggctgtg tttggacgga aaccaactgc aaggataacc atatccacgt
    19801 caaagctttc tttgtcagta atcaagcgtt caactttacc gtcaccttcg attgctttaa
    19861 cagtttgacc tagagccaag cggatgttgt gatcttccaa gttcttcgcc atcatttgtg
    19921 tgaagtcttt gtcatagtaa ccgttcaaga cagtatcaac gatatcaaca aggacaactt
    19981 cttttccaag acgttcaaag gcttcagcaa gttcaacacc gatgtaacca ccaccaacaa
    20041 cggcgatacg gtcgaggtgt tggctcttgt cagaaagttt attgataact tcttcagcat
    20101 tttggtacaa tttcacgaat tgtacgtttt caagagttgc tttaaattcg cggtttcctt
    20161 taacaatttc aacaccttcg attggtggca agattggtgt agaacctgta gcgaaaatca
    20221 atttttcgta tgattctttg tgctcttttc cttcaacttc cgctgtaact actttgttat
    20281 catagtcgat tgaaagaaca ggtgagttca tgtaaacttt agcaccttta gcttccaatt
    20341 tttctttatc agaatagaac aagccttcag caccgtcaat ttgttcacca atccaaagag
    20401 ccattccaca tcctaggaaa gagatgttag agttttggtc aaatacaaca atttcgttct
    20461 catttccaaa attatccaac atggtattga tacatgctgt accagcgtgg ttagcaccga
    20521 ctacaacgat tttactcata aaaatttcct gccttcattc taaatttacc ttactagtat
    20581 accatttacc gttagcgttt acaagggggg tactattatt tcagcatttt ttatcaaaaa
    20641 taattcccca tttatcctgt tttcaagcct catgaatttt atgagcatat ttcttgactt
    20701 tttccaaatt ttgtttgtga aaattctaac tttatggtat tataataaca tgaaaatgaa
    20761 atgtaagggc ttcaatttag atgattattt gatattacaa cttattttct ataagagaaa
    20821 ggagttggta gcttgactct tcgttcacat tctgaacggc taatggggac tactatcact
    20881 atttcattag tggatgagca agccgatatc tttctccaaa aatcctttga cttgctcaaa
    20941 gaacttgaat accgcttcaa cgccaatagt caagaatctg agttgatgga aatcaattat
    21001 caagcaggaa tagcccccgt cacggttcat ccagacctgt ttgagctgat ttcacttggg
    21061 ttagagcata gcctagcgct ctctagccac ctcaatatca gcattggtcc cttaattcaa
    21121 acctggcgta tcggtttttc agatgccaag gtcgcccagc ctcaagaaat tgaatcggtg
    21181 ctgcctctaa tcaatcctca tggtatcgag ttagattctt ctacttctac tgtgttttta
    21241 aaacagaaag gaatgaagat tgatctaggt tgtttagcca aaggatacag tgcggataag
    21301 gttgcccaat ttcttaggaa agagggggtg acttctgcct tgatcaatct gggagggaat
    21361 atcctgacca ttggaaaaaa tcaggcaaga ggggataacc catggcaaat cgggattcaa
    21421 gacccagcca atcctagggg aaatcattta atgaccatcc ctgttgtcaa taaatctgtc
    21481 gtgacttcag gcatttatga acgtcacctg accgtcgatg gacaagatta ccatcacatt
    21541 tttgacagtc aaacaggata tcctgttgaa acggaactag cgagtctaac aatcatctct
    21601 gataaatcag tcgatggcga aatctggacg actcgactct ttggagaaag acctgcttct
    21661 atcctctggc aagtcgaaag tttggagggc atcgaagtca tcctcatcga taaagaaggc
    21721 cacctaagct gttcttcagg aattcctact ctatagagac aactgtttca atggaatttt
    21781 aaaattgtat tatgtaaaaa gagaaaggaa atctcatgtt aaaacttatt gctattgttg
    21841 gaacaaattc aaaacgttct acaaaccgtc aattgcttca atacatgcaa aaacactttg
    21901 ctgacaaagc tgaaattgag cttgttgaaa tcaaggccat tcctgtcttc aacaaaccag
    21961 ctgacaagca agtacctgct gaaatattgg aaattgctgc taaaatcgaa gaggcagatg
    22021 gcgttattat cggtactcct gagtatgatc actctattcc agctgttttg atgagcgctc
    22081 ttgcttggtt gtcttatggt atttacccac ttttgaacaa accaatcatg attacaggtg
    22141 cttcttacgg tacgcttggt tcatctcgtg cccaattgca acttcgtcaa atcttgaatg
    22201 ctcctgaaat caaggcaaat gttcttccag atgaattctt gctctcacac tctcttcaag
    22261 catttaaccc aagtggcgac ttggttgacc ttgatgttat caagaaattg gatgccatct
    22321 ttgatgactt ccgtatcttt gtaaaaatca cagaaaaatt acgtaatgca caagaattac
    22381 ttcgcaaaga tgctgaagac tttgactggg aaaatttgta agataggaga ccaaaaagaa
    22441 tgaaatttgt tggacttgtt ggatcaaact acgatcaatc atataaccgt aagctcttgg
    22501 aatttatccg tcgccacttc aaactcaaat ttgaattaga agttcttgaa attgacgaag
    22561 ttccaatgtt taaccaagat gaaaaatggg atgaaagttt ccaattacgt tatttatata
    22621 acaaaattac tcgtgcagat ggtgttatca ttgctactcc tgagcataac cacactatct
    22681 cagcttcact caaatctgta cttgaatggc tttcatacga agttcatcca tttgaaaaca
    22741 agcctgttat gattgtgggt gcatcatact atgaccaagg aacatcacgt gctcaagttc
    22801 accttcgtaa aatccttgat gctccaggtg ttaatgccta cacgcttcca ggtaacgaat
    22861 tccttcttgg taaagctaag gaagcttttg ataacaacgg aaatatcacc aacgaaggca
    22921 ctgttaaatt ccttgaaact tgcttagata actttgttaa atacgtagga gtcgtttcga
    22981 aattgaaaaa accaaaacca atcgaatcag aagacttgga ttgtggaaaa ccaatcgcta
    23041 caaccatcac agaagttgac cctgacgatc cagaatgggt agaaaaagtt gcagcaatca
    23101 ctggtgctgt ttctggtgat acctatgtca aattggacca cggtatccta acagttaacc
    23161 aaattgatat gttcttgaaa gctatgccat ttgaattgac atacgctgac gacaacaacc
    23221 aattcctcta ctacaacaac gctcaccaag atccagacac tatgttcgct aaacgtgtac
    23281 cacctcaatc aggtagccgt atgtcgactg ttcatggttc tcttccacca gcacgtatga
    23341 agaacgtaga gtgggttatc ggaacacttc gcaacggaaa ccaagaatac gtccgtacga
    23401 tcgttccagg ttctcctgca ggtgttatca acactcacaa ctaccaagca atgtactatc
    23461 ctgatggatc atacgctggt atcaatgaaa tcgtctttaa cttccaacca tggcttgact
    23521 ggtacctaaa agagactggt caacgtttgg tgggtggtag cggaccattt gctcctgctg
    23581 ccggaggtca tggagacgcc gatgctactt ctggcgcttc tgattcaggg gatgctggag
    23641 gccacggtgg tgacgcagac gctacagccg gtgcaagtta ctaatgatag tttttaggaa
    23701 ccattatggt tcctttttta gcgaaaaaag actagcaaac acaaactgct agtctctttg
    23761 ttatcgtttc atctatcttt gttaaaagaa agagtacata ttatgaattt gggtcatcaa
    23821 gactacggcc atgtaatcct ttttcacgtt gtacttggcg tagtttttct ggtgtaacat
    23881 cgtttccttc ttcgtccaca attttgattc cttcaatgtg atgacgaaca gcgcgacgat
    23941 aaccttcgat gtactcctca cgtagtttgg cttgttccac tttttcttct agtgttaagc
    24001 cttctgtttt tttcttttta gcaagctcat tgatacgagc aatttttttc ggatccattt
    24061 cttctccttt gtaataagat aaagtccgtt tatcaaattt tatttagtgt taatttggtt
    24121 aaaacaagca aacttagctg ctttcttggt tagttgtgac aagattgcca aacgattttg
    24181 acggacagcc tgatcttcag ccattaccat agtattttca aagaaagcat caatgactgg
    24241 gctaagcgca aagagttgtt tcaattgctg acttgcaggt cctgataaaa cgagtgtttc
    24301 tactgcttct gccaaagctt tttcttggtc attctcaaat agtgctgaat caactgtagc
    24361 acccccttct gccttctcag ccaggttaaa ggcacgagaa agtgattcaa cagatggttt
    24421 aaaatcttct tccttgctta cttctacgag agcacttgct gcttccaaca tatctgccac
    24481 aacaaagttt gaacctgcaa gaactgcttc cttgatatct tttggagtag agcccatcat
    24541 cttatcaaca cgagccttga taaagtccat aacctctgct ttattttcat aagtcaaact
    24601 gtcaaatttc aatgcataaa ggctatcaat cagctcatcc atagcaatgt gccaaccaaa
    24661 ggcatccaag atacgaacca caccttgagt tgcacgacga agggcataag ggtcattaga
    24721 acctgatgga atcaatccta ctgagaagaa actcaaaatc gtatccaatt tgtctgcaat
    24781 ggctagaact gcgccgacct tgctctctgg aagttctcct tcagctgatg taggcatgta
    24841 gtgttcacga atagcagctg ccaccgctgg agtttcacca gcaagaaggg tgtatttttc
    24901 acccataatt ccttggagtt cgtcaaattc accaaccata cctgtcaata agtcaaactt
    24961 gtaaatggct gctgcacgag caaggtcaac tgtttcatcc actgacaaac cagctttttc
    25021 tgccaaaagt acagtgattt gacccgtacg aatcatgtgt tcacgaaggg aaccaatctt
    25081 ctcatggaag gtgacattgt ttaatttttc aacaagatct gaaatcacca atttttggtc
    25141 ttcacgccag aagaattctc cgtcttccaa gcgggctacc aagacttttt catttccttt
    25201 gatgacattt ttcaaacgct ctgcgtttcc gttacgaaca gaaatgaagt ttggcaagag
    25261 ttttccatct tgatcacgaa caacaaagta acgctggtgt tccttcatag aagtcaccaa
    25321 gacttcttct ggaacttcaa ggtatttagc atcaaaactt cccatgaagg cagttgggta
    25381 ttcaaccaaa ttcaagactt cattcagcaa atccgcatca atttcgatac gtacaccatg
    25441 ttttgcctca atttccttga tttggtcaac aatcatttgc tcacgttcac atggatctgc
    25501 gattacaaac tgcttacgaa ggtcttcttc atagctcaat gctgactgaa ttttggtttc
    25561 tttccccaag aaacgatggc cacggctcac acgacttccc ttgatatcaa ggaaatccaa
    25621 gtcaaactct tgctcatcca agagaacagt taaagtgtgg acaggacgaa tgtattcaaa
    25681 gctgtttcca gcccagtgca tgctgacagg gaaagtcaat gacttcaaga catctacaac
    25741 accaggaaca atggcttcaa ctgcttgacc aacttcttcc ttagtgacat agacatattc
    25801 ttcacccttg atttcacgga attcgatatc ttcaacagtc aagccttttc cacggacaaa
    25861 tccttgagct gctttggtga agtttccatc actatccaag gcaattttct ttgctggtcc
    25921 cttgaaatct tctgtcaaat cagactgttt gtctgataaa ccagtcacac gaacagccaa
    25981 acgacgtggt gttgagaagg tttgaatggc ttcaaaagac aggcgttttc ccttgaggaa
    26041 ggctgccatt ttttcgccta gttgtttttc acttggtgtg acaacatagg ctggtaattc
    26101 ttcaagaccg agttctacta ataagttttt tgtcatgttt tttctccgaa aagtatcttt
    26161 ttattttcca gtttgcctta tgtgattggc acgcaagcaa ccaacttcgt tgtttcattg
    26221 ctaaaattgg agccaaaagt ctccaatttt gcctagtatc cttagtttca ctaaggatac
    26281 cttcatcact gcggcaactg gctcagggct cactctgttc gcccattttc gtaatttgtc
    26341 actctcttta ttctgcgtct tctgctaaga gtttagctcg tgtttcttca tccaaaagtg
    26401 ggtagcctag tcgtttgcgt tctgcgacaa aggttttggc tacgacacgg gccaagttac
    26461 ggatacgagc gatatagcct gcacgctctg ttacagatac ggcaccacgc gcgtcaagca
    26521 gattaaaggt atgtgaacat ttgagaacat agtcataggc agggtgtacc aagccttctt
    26581 ctaatgcacg accagcttct ttttcaaact tatcaaaatt ttcaagcaac atttcttggt
    26641 ccgaaatttc aaatgaatat tttgagtgct catactcagg ctggataaag atttctccgt
    26701 attttacacc atcagcccac tcgatatcat agacagagtc tacttcttga atgtaagaag
    26761 ccaagcgctc caaaccatag gtaacttccg cagtcacagg gctagttgcc aatccaccga
    26821 cttgttggaa ataagtgaac tgagtgattt ccattccgtc aagccaaact tcccaaccaa
    26881 gaccagctga accagttgat gggttttccc agttgtcctc aacaaaacga atatcgtgct
    26941 ccaaaggatt gattcccaat ttttccaaag actcaaggta aagttcttgg atatttgatg
    27001 gagaaggctt catgaccacc tggaattggt ggtgttggta gagacggtta gggttttccc
    27061 cataacgacc gtcagcagga cgacgtgatg gctctacata agctgcattc catggctcag
    27121 gtccgatagc acgaaggaaa gtgtaaggac tcattgtccc cgcacctttt tcattgtcat
    27181 aagcctgcat aagcatacaa ccttggtcat tccaaaattg ttgcaaagtc aaaataattt
    27241 cttgaaatgt taatttctta gacattgaat actccttaat aaaaaatgat ttcttgcgac
    27301 acgagtctgc ctcgtcgatt atacgaggca agacgagtta gtactgcgtc tagctcagca
    27361 ccctagtgct gagcgtgggg cagtccgact gtaaggaggt ttctgccact gacgcagtga
    27421 aatgtcaatt tcttagacat tgtttactcc ttaataaaaa ataatttctt gcgacacgag
    27481 tctacctcgt cgattatacg aggcaagacg agttagtact gcgtctagct cagcacccta
    27541 gtgctgagcg tggggcagtc cgactgtaag gaggtctctg ccactgatgc agtgaaatgt
    27601 caatttctta gacattgttt actcctttag tttttatatt tctttttcaa ttaatttctg
    27661 ctgaacaaac caatctaaca agggaaaagt tttttctgta tttgcccata aatgataaga
    27721 atctaagacc gcttgtacac tacctaaatt ttctaccaac ttatctactg tcaaaaagct
    27781 gcgccagtat tcataaaaaa tacttgcata gttcttatca taagtcgaag aaccaaaatc
    27841 attcaatgaa tgccaactat acttcttctg aaaaagttct acgagagatt gattacaaat
    27901 tttttccgct tgaaattcct cttctgtcaa gaaatacttg cgactaatat attcaaccat
    27961 cccctcttcg aaccagatat aagagtcata accattcgtc tattatttgt ataggctgga
    28021 acagcgcttt ccctaataat ctgtgtagca gcattaaaat ctgaccaaat aacggcttgc
    28081 ggtaaatcac agactgcaag ttcattcttt aaaaaagatt gataatcttt caacttttca
    28141 gtatttctta ctacaaaatc acgaaatgta gctagttgac tatcgtcttt tacaagataa
    28201 aggttgtcca cggctctcct ttctttcaca caatagactg aataagcaaa agaaccgcgt
    28261 ccgacaatcc taggttgatt taacctaggg gcgtcagaaa cgcggttcca ccctaattta
    28321 ttacttcatt gtttttgaaa atactacaga aagcgccagc tcattatttt gccctacttg
    28381 gctcccacta tcccaagctc gcttgaagaa cgccataaga ctttctttcc tgctgactat
    28441 tatatcaaaa attagaataa tgtacaattc tttttatgat tttctagaaa tccacatcat
    28501 caactcgtgg agcacccgat tgaacagcaa tcgtttttaa ggtttctctc tcctcatgac
    28561 tcagttcaat tccaaagcaa tcaagattag cttgaatacg agaggttgtg acagattttg
    28621 gaagtggtaa aaatccttct gccaagctcc aggccaaggc tatctgagca atcgattttc
    28681 cgtgatttgc tgcgatttct tgcacttgct tgctatcaaa tagttctccc tgaccaaaag
    28741 gcccccaagc ttccaataaa attccctttt cacgacagta agctacgact tgatcttgat
    28801 acacacctgg cgccaagcga acttgattga ccgcaggaac gatagttgca gtttcaagca
    28861 aggcatccaa atgatgggga agaaaattgc taacgccgat agcacggatt ttcccttctt
    28921 gatagaggtc ttccatcgct ctccaaactt ccgcattgcg agttttccat gcgtcatttt
    28981 ctctgagcgg ttttgggttc ggccaatgaa tcaaatacaa atccaaataa tccaagccca
    29041 gtttttctat agatttttcc aaagcttgac gagtttgctc ataggtttgc tgactattcc
    29101 aaagcttggt agttacgaac atttcttcac gtggaactcc gctatctttg attgcttgac
    29161 caacactttc ttcattctga taaatcgccg ccgtatcaat atgacgataa ccagccttca
    29221 aggcttctaa cactgcatga taggcttctt ctccatcctt agccttaaaa gttccaaatc
    29281 ccaatactgg aatttctact ccattattta attgataggt attcatatgc ttctccttct
    29341 attttattca tcaattatag cataagacat tttttaagta gtactatctt ctgtaaaaaa
    29401 taagtctgtc aaatatctaa cagacctatt ttttcttgct tattcatcac gactatagta
    29461 cagctcatga gcttttaggc gagtattgag catctctggt atggtcacaa aggtataacc
    29521 ttgatttttc aaatactcaa tgacccttgg caaggcattg actgtcggac tgtgaatatc
    29581 atgcatcaaa acgatagagc cgttagctac ttgatgctga atttctgtca aaatagatgc
    29641 ttcattttta ctcttccagt ccagactatc cacatcccac atgataaagc tcaaatccaa
    29701 gctattgcga atatcatctg taatagcacc ataaggtgga cgcatgagtt tagaactaga
    29761 acccagcact ttagttagca catcctcagt atcagtaatc tgctttttag cttcatcaag
    29821 agagagttgc gagagaatcg gatggctcca actatggttt ccaacaacat gaccttcaga
    29881 ttttatcctc ttcaccaagt cctcattccc agaaacattt ttcccaagca caaagaaagt
    29941 cgctttaata tcatatttag ctagggtctc taatacctgc ggggtcgttg ctggatttgg
    30001 accatcatca aaggttagag cgacaacttt ttgatatttc ttatcaaagt aagattgata
    30061 caaggccgca tctttttcga gtaagtacga agattggata acatcaaaga aagcagatac
    30121 tggcaaggct atctcttcta aattttcaag cacaggactt ggataaagga taatctgact
    30181 atccttgtaa tcaaaattcc atgcagacaa gtcttggtca gagaagcttt ttacaatctg
    30241 ctcactctgg tcttgctcta tttttttatc ctctatgaag gaggtcaact cttttatcag
    30301 ctgttcctta gccttactag catctgaaaa cagttggtca agtgtaaaag gttgcccatc
    30361 ttctgttaag tgaacctttc ctagactggt cttttcagtc tcttcaatct taaaagcaac
    30421 taaatcatag atttgtttgg tcacattacg attaacgact ccctttaaac ctgactcttc
    30481 ttgctctgta tagtagaaaa caagattgtc cttactttct agcttgtcct taacatcttg
    30541 atttatcagc tccctaacag aggaaatcac tttctcccct tggagagggt aataggcaat
    30601 cacttcggct tgcccctgac gaaaatactc cttctgattt ccctcactca attgatcatc
    30661 tttctctttt ttgagcaatt caatcttttg ctcaaaactt ttttgctggt agatcttaaa
    30721 agcaataaat ccgcctaata ggcaaataga aattgctaaa ataccaatca aagccaataa
    30781 tacatgtctc gttttcccgt gtctgccacg tcccagtcta cttttatcca tatttatatc
    30841 ataacaaatt tgagcaataa tttcaatgat acaatttggc aatccacaat tcctctagca
    30901 agccaaagca tcttacatca actccaacat ccgtatctgt agagattatg tagttttcta
    30961 tctatggaat ttctagtaga ttgaagctag aatagtagac ctatacttct aaaacattgt
    31021 tagaaatcga tttgattgtc ctgatcgatt tgtcctgttc tcatttcatt ttactataaa
    31081 actatgaaaa gctaggtcat ctgagaagta attactatcg acaattgcga tagcgtatac
    31141 tagtgcgatt tgtttgtagc tctggtgggt atcaccttca cgttggcgaa ttttttctag
    31201 gttttgatta acctgactgc acagataagg ccataggcga ttgatgaaaa aattctgatg
    31261 atgatcctga atttcgatga taacttggat tccattatct aactccgcca agacgtctat
    31321 actagtatag aaatcttacg ctgagtaggg catggaaggc aagacgtgaa tgttacttcc
    31381 ctccaaaatc gtcacatttt ttgctggcaa gtccagcata tcgcggataa attgacaaat
    31441 cattagttgg gctgatacct ggatgtctga gaatcctcct tttcctcctt ctattatagc
    31501 acatttttaa atctaggttt gctagattct atgcttctat ctatttattc ggaaaagaaa
    31561 gctaatagct tatttgaaat tttctcctct ctgttggata gtcccgtcta tctatgttat
    31621 aatgaaagaa ggatttaaaa tcggaaaagg agtatttatg ctaaaattag gtgtcatcgg
    31681 aactggcgct atcagccatc atttcataga agcagcccat accagtggag aataccaact
    31741 ggtcgcaatc tattctagaa aactagaaac tgcagcaacc tttgcttctc gctatcagaa
    31801 tatccaacta ttcgatcaat tagaagtctt cttcaaatct tcttttgatt tagtctatat
    31861 cgccagtcca aactccttac attttgctca ggcaaaagct gccttgtctg ctggtaaaca
    31921 tgttattctc gaaaagccag ctgtcactca accacaagaa tggtttgatt tgattcaaac
    31981 agctgaaaaa aataactgtt ttatctttga agcagctcgt aattaccacg agaaagcctt
    32041 tactactatc aaaaactttt tagcagataa gcaagtgtta ggagcagatt tcaactatgc
    32101 caaatactct tccaagatgc ctgacttgtt ggctggacaa acaccaaatg tcttttcaga
    32161 ccgttttgct ggtggagccc tcatggattt ggggatttat cccctctatg ctgctgttcg
    32221 tctttttgaa aaagctaatg acgcgactta ccatgctcaa cagcttgaca atagcattga
    32281 cctaaatgga gatggtatcc tcttctaccc agactatcaa gttcacatca aggctggaaa
    32341 aaacatcact tccaatcttc cttgcgagat ttatacaaca gatggaacct tgactctcaa
    32401 cacgattgag catattcgct cagctatttt taccgaccac caaggaaatc aagtccagat
    32461 tcctatccaa caggctcctc atacgatgac tgaggaagtc gctgcatttg cacacatgat
    32521 ccagcaacca gatctgaatc tctaccaaac ttggctgtat gatgcaggct ctgttcatga
    32581 gctactatat accatgcgcc agactgctgg cattagattt gaggcagaaa aatgaaaacc
    32641 aaactaccga ctgaatggca agaactgagt gaccaactcg gtttccaaga attcaccccc
    32701 attcaaactc aactatttga gccccttctt gctggagaaa acctcctagg agtgagccaa
    32761 accggaactg gtaagaccct agcgtatctc ctaccaagtc ttctcagact acaaaagaaa
    32821 aaagcccaac aactcttgat tctagcacca aacacggaac ttgctggaca gatttttgat
    32881 gtatgtaaaa cgtgggcaga agctatcgga ttgactgctc aactcttcct ttcaggttca
    32941 agtcagaaac gacaaattga acgtcttaaa aaggggccag aaattctgat tggaactcct
    33001 ggccgtatct ttgaactcat taaacttaaa aaaatcaaga tgatgaatgt ggaaaccatc
    33061 atcctggatg aatttgacca attactagat gattctcaga ttcactttgt agaaaaaatc
    33121 actcactacg cacctcgtga ccaccaactg gtctacatga gtgccacgac caagtttgat
    33181 caagaaaaga ttgtgccgaa tacacgcact attgatctct ctgatcaaaa attggacaat
    33241 atccagcatt tctacatgca ggtagaccaa cgtcatcgag tggatatact acgaaaactg
    33301 gctcatgtag aggatttccg tggtctagtc ttcttcaata gcctgtcaga ccttgggaat
    33361 gccgaggaaa aactacagta tcgtgatata ttggctgttt ccctcgctag tgatgttaat
    33421 gttaaattta gaaaagtcat cttagaaaag tttaaagata accaactaac cctgcttctt
    33481 gcaactgacc ttttggctcg tggaattgat atcgatagcc tagaatgtgt cgtaaacttt
    33541 gatattccta gagatagcga aacctacaca caccgtgctg gccgtactgg ccgtatgggc
    33601 aaggaaggat atgttattac tctcgtcact catccagaag aaatcaaaaa acttaaaaag
    33661 tttgcaagta tccgagaaat tgtcctaaaa aaccaagaac tctatatcaa ataaagcccc
    33721 attgctctat acacatccag tgtatggagc ttttttataa gcaattcaat tttatactca
    33781 tctgctttca aaaagcattc tagtccatct ctgattaacg atggacttta tcacctcctt
    33841 ctccagtcct tgtatgacat cttgaagttg attcatgaca tcttccaaag ttcgaaaggc
    33901 tttattctta aatccacgt
//
LOCUS       DBERDK010000016        33724 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.016, whole genome shotgun sequence.
ACCESSION   DBERDK010000016 DBERDK010000000
VERSION     DBERDK010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 33724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33724
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.016"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            128..886
                     /locus_tag="ACL73L_000749"
     CDS             128..886
                     /locus_tag="ACL73L_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGS0771760.1"
                     /translation="MALVEFKNVEKYYGDYHAFRNINLRFEKGQVVVLLGPSGSGKST
                     LIRTINGLETVDKGSLLVNGHQVAGASQKDLVPLRKEVGMVFQHFNLYPHKAVLENVT
                     LAPIEVLGIDKKEAEKTAQKYLEFVNMWDKKDSYPAMLSGGQKQRIAIARGLAMHPEL
                     LLFDEPTSALDPETIGDVLAVMQKLAHDGMNMIIVTHEMGFAREVADRIIFMADGEVL
                     VDTTDVDNFFDNPSEPRAQQFLSKIINHESDKVK"
     gene            926..1690
                     /locus_tag="ACL73L_000750"
     CDS             926..1690
                     /locus_tag="ACL73L_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HGS0771761.1"
                     /translation="MISLFGLAAAKPVQADTSIADIQKRGELVVGVKQDVPNFGYKDP
                     KTGTYSGIETDLAKMVADELKVKIRYVPVTAQTRGPLLDNEQVDMDIATFTITDERKK
                     LYNFTSPYYTDASGFLVNKSAKIKKIEDLNGKTIGVAQGSITQRLITELGKKKGLKFK
                     FVELGSYPELITSLHAHRIDTFSVDRSILSGYTSKRTALLDDSFKPSDYGIVTKKSNT
                     ELNDYLDNLVTKWSKDGSLQKLYDRYKLKPSSHTAD"
     gene            1703..2380
                     /locus_tag="ACL73L_000751"
     CDS             1703..2380
                     /locus_tag="ACL73L_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGS0771762.1"
                     /translation="MTDLSSWTAYFQDFGQFFNGFLFTLALAVGSFILAMVLGIFFGA
                     MSTSKRPILRILARIFVEFYQNTPLLVQFVIVFYGLPLISDHIIMIPIYWTAVLCVGL
                     YHGAYIAEVIRSGIQSIPSGQMEAALSQGFTYISAMRLIILPQAFRIILPPLTNQIVN
                     LIKNTSTVAIISGVDLMFVTKSWSALNGNYIPAFLGAALLYFALCFPVAQFGRKMEQA
                     NKKAYSL"
     gene            2390..3049
                     /locus_tag="ACL73L_000752"
     CDS             2390..3049
                     /locus_tag="ACL73L_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGS0771763.1"
                     /translation="MESILEVLTPDNLVFIFKGFGLTLYISLIAIILSTIIGTVLAVT
                     RNGKNPVLRIISSIYIEFVRNVPNLLWIFTIFLVFKMKSTPAGITAFTLFTSAALAEI
                     IRGGLNAVDKGQYEAGMSQGFTSAQILYYIILPQAIRKMLPAIISQFVTVIKDTSLLY
                     SVIALQELFGASQILMGRYFEPEQVFSLYILIALIYFSFNLAISSLSHMLAKRWQQAA
                     E"
     gene            3307..4497
                     /locus_tag="ACL73L_000753"
     CDS             3307..4497
                     /locus_tag="ACL73L_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HGS0771764.1"
                     /translation="MKSVKGLLFIIASFILTLLTWMNTSPQFMIPGLALTSLSLTFIL
                     ATRLPLLESWFHSLEKVYTVHKFTAFLSIILLIFHNFSMGGLWGSRLAAQFGNLAIYI
                     FASIILVAYLGKYIQYEAWRWIHRLVYLAYILGLFHIYMIMGNRLLTFNLLSFLVGSY
                     ALLGLLAGFYIIFLYQKISFPYLGKITHLKRLNHDTREIQIHLSRPFNYQSGQFAFLK
                     IFQEGFESAPHPFSISGGHGQTLYFTVKTSGDHTKNIYDNLQAGSKVTLDRAYGHMII
                     EEGRENQVWIAGGIGITPFISYIREHPILDKQVHFYYSFRGDENAVYLDLLRNYAQKN
                     PNFELHLIDSTKDGYLNFEQKEVPEHATVYMCGPISMMKALAKQIKKQNPKTELIYEG
                     FKFK"
     gene            complement(4818..5699)
                     /locus_tag="ACL73L_000754"
     CDS             complement(4818..5699)
                     /locus_tag="ACL73L_000754"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HGS0771765.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFFADGVKAI
                     QASVEERIDVFGSEGKA"
     gene            complement(5840..7168)
                     /gene="vncS"
                     /locus_tag="ACL73L_000755"
     CDS             complement(5840..7168)
                     /gene="vncS"
                     /locus_tag="ACL73L_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HGS0771766.1"
                     /translation="MKRTGLFAKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGGTMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYARTIVAPILEIKRVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     HEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKDRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDRETIDLLQMTQNLVKDYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSF
                     SDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENSLTFFIDFPKVVQD"
     gene            complement(7165..7821)
                     /gene="vncR"
                     /locus_tag="ACL73L_000756"
     CDS             complement(7165..7821)
                     /gene="vncR"
                     /locus_tag="ACL73L_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HGS0771767.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKDTKVDFESYSASLAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDAVWKATDEVPFDRVIDVYIKELRKKLDLDCILTVRNVGYKLERK
                     "
     gene            complement(7870..7953)
                     /gene="pep27"
                     /locus_tag="ACL73L_000757"
     CDS             complement(7870..7953)
                     /gene="pep27"
                     /locus_tag="ACL73L_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198524447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HGS0771768.1"
                     /translation="MRKEFHNVLSSGQLLADKRPARDYNRK"
     gene            complement(8133..9512)
                     /gene="vex3"
                     /locus_tag="ACL73L_000758"
     CDS             complement(8133..9512)
                     /gene="vex3"
                     /locus_tag="ACL73L_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HGS0771769.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGEDIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPETKKNLTADRAKRFGSSLMITGVNDSSKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLAAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIHTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQASILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLALVLVVLVMALASSNLLRKQ
                     PKELLLDGE"
     gene            complement(9564..10211)
                     /gene="vex2"
                     /locus_tag="ACL73L_000759"
     CDS             complement(9564..10211)
                     /gene="vex2"
                     /locus_tag="ACL73L_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HGS0771770.1"
                     /translation="MTLLQLQDVTYRYKNTAEAVLYQINYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDIRKKGYSYHRMHHISLVFQNYNLIDYLSPLENIR
                     LVNKKASKNTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            complement(10224..11501)
                     /locus_tag="ACL73L_000760"
     CDS             complement(10224..11501)
                     /locus_tag="ACL73L_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS0771771.1"
                     /translation="MNPIQRSWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VESNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELETVAKLKDKEAVT
                     GEQSVERDDLSAADNNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGDSKKILIHEE
                     LAKKNGLSLHDKIGLDAGQSESGKGQTVEFEIIGIFSGKKQEKFTGLSSDFSENQVFT
                     DYESSQTLLGNSEAQVSAARFYVENPKEMDGLMKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGAGALILVLSLWLRERVYEVGILLALGKGKSSIFLQF
                     CLEVVLVSLGALLPAFVAGNAITTYLLQTLLASGDQASLQDTLAKASSLSTSILSFAE
                     SYVFLVLLSCLSVALCFLFLFRKSPKEILSSIS"
     gene            complement(11858..12028)
                     /locus_tag="ACL73L_000761"
                     /pseudo
     CDS             complement(11858..12028)
                     /locus_tag="ACL73L_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron maturase-specific
                     domain-containing protein"
     gene            complement(12171..12770)
                     /locus_tag="ACL73L_000762"
     CDS             complement(12171..12770)
                     /locus_tag="ACL73L_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771772.1"
                     /translation="MQILCYFTITVVAKPNNSGEVHLDVSIEDNQGGSGHNFSSVSSS
                     SQTAKYEETGYNNNSSLYITIDKTSDATALLKLKLNNVDNQPATEVPSSGITVKLNAK
                     DNAGNWTSASNKKEITVKIVSAKPTYPDKILVKNPDNIKDTEKNAIIEKLKEANKNHP
                     AGAPTFAKGEGEHANDIVATYSDGTTYYVPLNDVTKYAR"
     gene            complement(12907..13791)
                     /locus_tag="ACL73L_000763"
     CDS             complement(12907..13791)
                     /locus_tag="ACL73L_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGS0771773.1"
                     /translation="MREKELQDWFPDARISDQPVEKEGYLTLPLASQQWILLEEAGLS
                     EREKQLVALLTQQEQARSLNPWYSYLVEGKGQAPQVFKKIQLVYCHLSYFQQENLASW
                     LDMMRTLFPNCQTVLQVGAQDYVFVLQQDKYTSVTDILSDMIEAVEYDFGLRLSIMLG
                     QVWSQTGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKPIKECL
                     HQMILDQDQIQEIILSLWENSAVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
     gene            complement(13795..14181)
                     /locus_tag="ACL73L_000764"
     CDS             complement(13795..14181)
                     /locus_tag="ACL73L_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HGS0771774.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLIALMLEEQVLTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLHMTENLERLESLVSWCIQKVEG"
     gene            complement(14174..15517)
                     /gene="cysS"
                     /locus_tag="ACL73L_000765"
     CDS             complement(14174..15517)
                     /gene="cysS"
                     /locus_tag="ACL73L_000765"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HGS0771775.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRYFEYRGYKVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGVKPATRHPRVVEFMADIIRFVEDLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLELGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFATADQIRDQLVTQGIKLLDTKDGVRWTRD"
     gene            complement(15599..16483)
                     /locus_tag="ACL73L_000766"
     CDS             complement(15599..16483)
                     /locus_tag="ACL73L_000766"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS0771776.1"
                     /translation="MIQARNKLSQEELSEAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGELVGLLTVYADDQDVEVTILVHPGHRRQGIARALFTSFERETASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRPYPLANVSHLEVLLADSSY
                     QDQISQLKFQAFSEEHESREVVDRYVAEALKDPESRLYILLKAGQVIGTCTVDLSTNT
                     NYLYGLAILEPERGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            complement(16495..17112)
                     /gene="cysE"
                     /locus_tag="ACL73L_000767"
     CDS             complement(16495..17112)
                     /gene="cysE"
                     /locus_tag="ACL73L_000767"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HGS0771777.1"
                     /translation="MGWWRETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFLW
                     KHGFKLLARMYSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRLHGKKDEPVIHEVEEKREYYVNKLEQAKDASHRSSGL"
     gene            complement(17128..19341)
                     /gene="pnp"
                     /locus_tag="ACL73L_000768"
     CDS             complement(17128..19341)
                     /gene="pnp"
                     /locus_tag="ACL73L_000768"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HGS0771778.1"
                     /translation="MAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDGQII
                     INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQE
                     EIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQVVKDQVT
                     AVYEEKYADHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVV
                     DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDPEYKKRFMHHYNFPQYSVGE
                     TGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRPELAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKV
                     DEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
                     DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHQKDYKPKKEFTETSKDSE"
     gene            19575..19844
                     /locus_tag="ACL73L_000769"
     CDS             19575..19844
                     /locus_tag="ACL73L_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine methyltransferase"
                     /protein_id="HGS0771779.1"
                     /translation="MLGSLSFFLRNETKIQLLKILSRQATQRKIGNRTTERLLLGRFI
                     FFHRVVGKFSFQDTSLERFNTKVSKAFTLIAIGRLAKCFTNSLVK"
     gene            complement(20026..20892)
                     /gene="metF"
                     /locus_tag="ACL73L_000770"
     CDS             complement(20026..20892)
                     /gene="metF"
                     /locus_tag="ACL73L_000770"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HGS0771780.1"
                     /translation="MSRQTPSLSFEVFPPNPAVGNDNIISALQDMQELAPHFISVTAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIELIKERAPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
                     DAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     LPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDIAGVHLYTMNNADTAKYIHQ
                     ATHALFNHQSLG"
     gene            complement(20956..23205)
                     /gene="metE"
                     /locus_tag="ACL73L_000771"
     CDS             complement(20956..23205)
                     /gene="metE"
                     /locus_tag="ACL73L_000771"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HGS0771781.1"
                     /translation="MSTTIVGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPASVQNLDLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGVKADDFVDSLVSAYQEVFAKLAELGATRIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLLQTYFGDVRDVYADLVNLPVDAIGLDFVEGKKTLELVK
                     GGFPADKTLYVGIVNGKNIWRNNYEKSLTVLEQIPAENIVLTSSCSLLHVPFTTANEE
                     FEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFATERVGENAELRARIAG
                     LTDADYTRLPAFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVH
                     STVAPDTQIHTHMCYSEFTDIIPAIDNMDADVISFEASRSNLEILDELKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEVILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
     gene            complement(23465..23731)
                     /locus_tag="ACL73L_000772"
     CDS             complement(23465..23731)
                     /locus_tag="ACL73L_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGS0771782.1"
                     /translation="MEGCLSELFSKEEILESDMRVAIMSELIEARNKQGISQKKLEEV
                     SGVSQPVIARMETGKTSPQLDTVLKVLASLGKTLAVVPLEQGKS"
     gene            complement(23816..24305)
                     /locus_tag="ACL73L_000773"
                     /pseudo
     CDS             complement(23816..24305)
                     /locus_tag="ACL73L_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            24461..25461
                     /locus_tag="ACL73L_000774"
                     /pseudo
     CDS             24461..25461
                     /locus_tag="ACL73L_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597989.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
     gene            complement(25529..27934)
                     /gene="pheT"
                     /locus_tag="ACL73L_000775"
     CDS             complement(25529..27934)
                     /gene="pheT"
                     /locus_tag="ACL73L_000775"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HGS0771783.1"
                     /translation="MLVSYKWLKELVDIDVPSQELAEKMSTTGIEVESVESPAADLSK
                     IVVGEVLSCEDVPETHLHVCQVNVGEEEARQIVCGAPNVRAGIKVMVALPGARIADNY
                     KIKKGKIRGLESLGMICSLGELGISDSVVPKEFADGIQILPEDAVPGEEVFSYLDLDD
                     EIIELSITPNRADALSMCGVAHEVAAIYDKAVNFKEFTLTETNEAAADALSVIIETDK
                     APYYAARILDNVTIAPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDN
                     FEGTDIRVREARAGEKLVTLDGEERDLDVNDLVITVADKPVALAGVMGGQATEISEKS
                     SRVVLEAAVFNGKSIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASLIAELAGA
                     TVRKGIVSAGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFT
                     VRVPRRRWDITIEADLFEEIARIYGYDRLPTSLPKDDGTAGELTVTQKLRRQVRTIAE
                     GAGLTEIITYALTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDTVAYNV
                     ARKNKNLALYEIGKVFEQTGNPKEELPNEINSFAFALTGLVAEKDFQTAAVPVDFFYA
                     KGILEALFTRLGLQVTYTATSEIASLHPGRTAVISLGDQVLGFLGQVHPVTAKAYDIP
                     ETYVAELNLSAIEGALQPAVPFVEITKFPAVSRDVALLLKAEVTHQEVVDAIQAAGVK
                     RLTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEEKVNA
                     EVR"
     gene            complement(28011..28520)
                     /locus_tag="ACL73L_000776"
     CDS             complement(28011..28520)
                     /locus_tag="ACL73L_000776"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS0771784.1"
                     /translation="MIRKVEMADVEVLAKIAKQTFRETFAYDNTEEQLQEYFEEAYSL
                     KTVSTELGNPDSETYFIMHEEEIAGFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGF
                     GLGKQLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDT
                     DWLLRKKLR"
     gene            complement(28520..29566)
                     /gene="pheS"
                     /locus_tag="ACL73L_000777"
     CDS             complement(28520..29566)
                     /gene="pheS"
                     /locus_tag="ACL73L_000777"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HGS0771785.1"
                     /translation="MSTIEEQLKALREETLASLKQITAGNEKEMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTL
                     PGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
     gene            complement(30157..31572)
                     /locus_tag="ACL73L_000778"
     CDS             complement(30157..31572)
                     /locus_tag="ACL73L_000778"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HGS0771786.1"
                     /translation="MTIFPDDFLWGGAVAANQVEGAYNEDGKGLSVQDVLPKGGLGEA
                     TENPTEDNLKLIGIDFYHKYKEDITLFSEMGFNVFRTSIAWSRIFPKGDEEEPNEAGL
                     KYYDELFDELHAHGIEPLVTLSHYETPLYLARKYHGWVDRRMIHFYEKFARTVLERYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDIPKENLSKQELYQAIHHELVASSLVTKIAREI
                     NSEFKVGCMVLAMPAYPMTPNPKDVWATHEYENLNYLFSDVHVRGYYPNYAKRYFKEH
                     DINIEFAAEDAELLKNYTVDFLSFSYYMSVTQSALPTQYNSGEGNIIGGLVNPYLESS
                     EWGWQIDPIGLRIILNRYYDRYQIPLFIVENGLGAKDQLIKDELNNLTVQDDYRIQYM
                     KEHLLQVAEALQDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGNGTFNRY
                     KKMSFTWYKGVIESNGESLFK"
     gene            complement(31585..33423)
                     /locus_tag="ACL73L_000779"
     CDS             complement(31585..33423)
                     /locus_tag="ACL73L_000779"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HGS0771787.1"
                     /translation="MSYKDTVQKILDVIGGEKNVNRVTHCVTRLRLELKDENLVNDDD
                     VKKIPGVIGIMKKNGQYQIILGNDVANYYKEFVKLGNFESDSVVQGHKGNILERIIEY
                     IAGSMTPIIPAMLGGGMLKVLVIILPMLGILQSDSQTIAFLTFFGDAPYYFLPLLLAY
                     SASQKLKVTSTLAMSVAGVLLHPNFVQMVQSGNPLSLFVVPVTPASYGSSVVPILIMV
                     WLMKYIEKMIAKLTPAVTKSFLQPTLVLLVSSCIALVVVGPIGVIVGEGLSNLVGQMY
                     GVAGWLTLAILGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAAS
                     MAVALKSKNNNTKQIAFAAGFSALLAGITEPALYGVTLKYKKPLYAAMIGGGLAGLFA
                     GLTSVKAYLFAVPSLIALPQFIYSDVPSNIVNALIVAVISVVITFVLAYIFGIDEEES
                     SSNLEVKAGVSNKKMIFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAPFD
                     GKITNIFPTKHAIGLKSDEGVELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIFEM
                     DLNLIKTKGYETVTPVIVTNTNDFLDVLVLPNNQTIEHSKELLVIL"
     gene            complement(33441..>33724)
                     /locus_tag="ACL73L_000780"
     CDS             complement(33441..>33724)
                     /locus_tag="ACL73L_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HGS0771788.1"
                     /translation="QDFYCTEFNQDSIDYYRFITHIKLFAHRLVENTTYCDDDDEDLL
                     ALMKNKYPREYECGEQVAMFIQTEYNYLLTSSELVYLMAHIRRLTKNLD"
BASE COUNT         9670 a         7513 c         6235 g        10306 t
ORIGIN      
        1 tttgactgtc ccgatcgatt tttcctgttc ttatttcact atagtgaatt ttttcagata
       61 tgatttgaca agaacattct tttattgtag aaccgtgtta taaaatataa cacaatagag
      121 atctctcatg gctttagtag aatttaaaaa cgtcgaaaaa tattacggag actaccacgc
      181 attccgcaac atcaatctcc gttttgaaaa aggacaagtt gttgtcctgc ttggaccttc
      241 tggctctggg aagtccactc ttatccgtac gatcaatggt ttagagactg ttgacaaagg
      301 aagtctccta gtcaatgggc accaagttgc tggtgccagc cagaaagatt tggtacctct
      361 tcgcaaggaa gtcggcatgg tttttcaaca ttttaacctt tatccacaca aagctgtgtt
      421 agaaaacgta acgcttgcac ccattgaagt tctaggaatt gataaaaaag aagctgaaaa
      481 aaccgcccaa aaatatctgg aatttgtaaa tatgtgggac aagaaagatt cctatcccgc
      541 catgctatct ggtggacaaa aacagcggat cgccatcgct cgtggtcttg ctatgcatcc
      601 ggaactcctc ctctttgatg aaccaacatc tgctcttgat cctgagacta tcggagatgt
      661 tctagcagtt atgcagaaac tggcgcatga tgggatgaac atgatcatcg ttacccacga
      721 aatgggcttt gctcgagagg ttgcggaccg cattatcttt atggccgacg gagaagtttt
      781 agtagatacg acagatgtcg ataacttttt tgacaatcca agcgaacctc gtgcccaaca
      841 attcctcagc aaaattatca accacgaaag tgacaaagtc aaataaggag gcgcctatga
      901 aaaaaaattc tttttatcag cattattgat tagccttttc ggccttgctg ctgccaaacc
      961 agtccaggct gatacaagta tcgcagacat tcaaaaaaga ggcgaactgg ttgtcggtgt
     1021 caaacaagac gttcccaatt ttggttacaa agatcccaag accggtactt attctggtat
     1081 cgaaaccgac ttggccaaga tggtagctga tgaactcaag gtcaagattc gctatgtgcc
     1141 ggttacagca caaacccgcg gcccccttct agacaatgaa caggtcgata tggatatcgc
     1201 gacctttacc atcacggacg aacgcaaaaa actctacaac tttaccagtc cctactacac
     1261 agacgcttct ggatttttgg tcaataaatc tgccaaaatc aaaaagattg aggacctaaa
     1321 cggcaaaacc atcggagtcg cccaaggttc tatcacccaa cgcctgatta ctgaactggg
     1381 taaaaagaaa ggtctgaagt ttaaattcgt cgaacttggt tcctacccag aattgattac
     1441 ttccctgcac gctcatcgta tcgatacctt ttccgttgac cgctctattc tatctggcta
     1501 cactagtaaa cggacagcac tactagatga tagtttcaag ccatctgact acggtattgt
     1561 taccaagaaa tcaaatacag agctcaacga ctatcttgat aacttggtta ctaaatggag
     1621 caaggatggt agtttgcaga aactttatga ccgttacaag ctcaaaccat ctagccatac
     1681 tgcagattaa ggaggacacc ccatgacaga tttatcatct tggacagcct attttcagga
     1741 ttttggacaa tttttcaatg gtttcctctt cacccttgcc ctagcggttg gttcctttat
     1801 cctcgctatg gttttgggca tcttctttgg tgccatgtca accagtaaac gtccaatttt
     1861 acgcatttta gctcgcatct ttgttgaatt ttaccaaaat actcccctct tggtgcagtt
     1921 tgttattgtt ttttatggcc tacctcttat cagtgaccat atcatcatga ttccaattta
     1981 ttggacagcc gttctctgcg tgggactcta tcacggcgct tatatcgctg aggttattcg
     2041 ttcagggatt cagtctattc ctagtggtca gatggaggcc gccttgtcgc aaggttttac
     2101 ctatatcagt gccatgcgct tgattatctt gcctcaggcc ttccgcatca ttctccctcc
     2161 attgaccaac caaattgtta acctcatcaa gaacacctct actgtagcta tcatctctgg
     2221 agtagacttg atgtttgtga ctaagtcttg gtcggctctc aacggaaact atataccagc
     2281 ctttttaggt gctgctcttc tctactttgc tctatgcttc cctgttgccc agtttggtcg
     2341 caagatggag caagccaaca aaaaagccta ttcactttag gaggttacta tggaatctat
     2401 tttagaagtt ttaaccccag ataacctagt ctttatcttt aaaggatttg gcttgaccct
     2461 ctatatttct ctgattgcca tcatcctctc tactatcatc ggtacggtgc tagctgtcac
     2521 gagaaatggc aaaaatcctg tcttacgcat tatttccagt atttatatcg agtttgtgcg
     2581 caacgttccc aaccttctct ggatttttac tatctttttg gtgttcaaaa tgaaatccac
     2641 accagcaggt attacagcct ttactctctt tacatcagca gccttggctg agattattcg
     2701 aggcggtctc aatgccgtag acaagggaca gtacgaagca ggaatgtcac aaggcttcac
     2761 ctcagcccaa atcctctact acatcattct cccacaagcc atccgcaaaa tgctaccagc
     2821 catcatttct cagtttgtta ccgtgattaa ggataccagt ctcctctact ctgttatcgc
     2881 cctacaagaa ctctttggag ccagccaaat tctcatgggc cgttatttcg aaccagagca
     2941 ggtcttcagt ctttacatcc tgattgccct catctacttc agctttaacc tagcaatttc
     3001 tagcctgtct catatgctag ccaaacgttg gcaacaagct gcagaataac actcttcgaa
     3061 aatctcttca aaccacgtca gctctatctg caacctcaaa acagtgtttt gagcaacttg
     3121 cggctagctt cctagtttgc tctttgattt tcattgagta taaacagcca ctctccagtt
     3181 caattggaga gttttttgat gagagtagat atttatagac ctcaatttga caccatacat
     3241 tttctatgat aaaatgtaac aaatttattt acaacaaatc gaaatctaaa tgaaatggaa
     3301 ttttctatga aatcagtcaa aggactactc tttatcatag ctagttttat cttgactctt
     3361 ttgacttgga tgaacacttc tccccaattc atgattccag gactagcttt aacaagccta
     3421 tctctgactt ttatcctagc cactcgtctc ccactactag aaagctggtt tcacagtttg
     3481 gagaaggtct acaccgtcca caaattcaca gcctttctct caatcatcct actaatcttt
     3541 cataacttta gtatgggcgg tttgtggggc tctcgcttag ctgctcagtt tggcaatctt
     3601 gccatctata tctttgccag catcatcctt gtcgcctatt taggcaaata catccaatac
     3661 gaagcttggc gatggattca ccgcctggtt tacctagcct atattttagg actctttcac
     3721 atctacatga taatgggcaa tcgtctcctt acatttaatc ttctaagttt tcttgttggt
     3781 agctatgccc ttttaggctt actagctggt ttttatatca tttttctata tcaaaagatt
     3841 tccttcccct atctagggaa aattacccat ctcaaacgct taaatcacga tactagagaa
     3901 attcaaatcc atcttagcag acctttcaac tatcaatcag gacaatttgc ctttctaaag
     3961 attttccaag aaggctttga aagtgctccg catccctttt ctatctcagg aggtcatggt
     4021 caaactcttt actttactgt taaaacttca ggcgaccata ccaagaatat ctatgataat
     4081 cttcaagccg gcagcaaagt aaccctagac agagcttacg gacacatgat catagaagaa
     4141 ggacgagaaa atcaggtttg gattgctgga ggtattggga tcaccccctt catctcttac
     4201 atccgtgaac atcctatttt agataaacag gttcacttct actatagctt ccgtggagat
     4261 gaaaatgcag tctacctaga tttactccgt aactatgctc agaaaaatcc taattttgaa
     4321 ctccatctaa tcgacagtac gaaagacggc tatcttaatt ttgaacaaaa agaagtgccc
     4381 gaacatgcaa ccgtctatat gtgtggtcct atttctatga tgaaggcact tgccaaacag
     4441 attaagaaac aaaatccaaa aacagagctt atttacgaag gattcaagtt caaataagta
     4501 atcatactct cctgggattt cccaggagag ttttttatag ccaaatgaaa taagacagga
     4561 acgatggaat tttggcattc aaatcaattg ataagactat tttatagtgg agtgaaatag
     4621 gatacgaaca aattgattag gaaaatcaaa tgaatttata gaaatctttt agcagttatg
     4681 ttatcctatt ctagtttcaa aacgctatag aagcagcatt gtgctagtct agattcagtt
     4741 tactatacta aaacgagtag cttgaaatca aaaaacccac cctcacaggc aggttttatc
     4801 tgtattattc agctagatta tgctttacct tctgaaccga atacgtcgat acgttcttca
     4861 accgatgctt ggatagcttt tacaccgtca gcaaagaatt tacgtgggtc gaagagtttt
     4921 ttcttgtcgt attctgcttc gtttgcttcg tagtcacgag caaatttacg agttgcgtta
     4981 gcgaatgcga tttggcattc tgtgttaacg ttaactttgg caacaccaag tttgatagct
     5041 gcttggattt gctcatcagg aatacctgat ccaccgtgca atacgattgg gaatcctgga
     5101 agagcttctg tcaatttttg caagtggtca aggtcaagac cttcccagtt tactgggtaa
     5161 ggaccgtgga tgttaccgat accagctgcc aagaagtcga taccagtttc aaccattgct
     5221 ttagcgtctt cgattggagc caattcacct ttaccgatga ttccatcttc ttcaccaccg
     5281 atagtaccaa cttcagcttc tactgagata cctttagcgt gtgctttttc aacaacttct
     5341 ttagccaatt taaggttttc ttcaactgga aggtgtgaac cgtcaaacat gattgaagta
     5401 taaccaactt cgatacactc aagtgcatct tcgtagtgac cgtggtcaag gtggatagct
     5461 actggtacag tgatacccat tgattcaaca aggttagcga tcaagttgcg agcaactttg
     5521 taaccaccca tgtatttagc agcacccatt gaagtttgga tcaaaactgg agctttttta
     5581 gcttctgctg cgcgcaagat agcttgagtc cactcaaggt tgtttgtgtt aaatccacca
     5641 actgcataac cgttgtcacg ggctgcttgg acaaattttt ctgctgaaac gattgccatt
     5701 ttatcaggcc tcctgtatat ttttatgggt catcccattt acattgttca ttttatcact
     5761 ttttgccaaa aaaatctagt ttttcccgca gtttcgattg attttcttct aactccatct
     5821 atgtaaaccc tttctctccc tagtcttgga cgacttttgg aaaatctata aagaaggtta
     5881 aactattctc ctccatctcg aaacgataag ctaatttttc atgttctaat agactcttaa
     5941 ccacaaagag ccccatacca gaccccttga ccttgcgact ggcattgtca gaaaaagact
     6001 gggctagttt ttcttgttcc tctgagctac agctattttc gataaaaagt tctccttctc
     6061 tttctccaat tcgaactaag ccacctggaa cagagtgctt aatggcattg ctgatgagat
     6121 tagaaagaat caacttcata actgatgggt ttagataagc ctgctgatgg gtcaaactat
     6181 tgtctatctg gagctctctt tccttggcta gcaaggcata atctttgacc agattttgcg
     6241 tcatctggag gaggtcaatt gtttccctat catctcgcaa ttcctgcaca gaagagaggg
     6301 aaagtatctg cagaacatgg tgattgagtt catccacaat ccccaaggca actcccagat
     6361 actggtctct atccttataa cgaccgatat tctctctcat attttcgatt aggattttca
     6421 aactagccag cggtgttttc aattcatgag aagctcctcg taggaattcg accttcatct
     6481 tctccagctg gagaatggct tcattctttt catgcaagtc cgcaataaca gtcaagagat
     6541 gctggtagag gctattgatt tgttccttga gattacctat ctcatcctta gaatccacgc
     6601 gcaatcgcac ttgggaatcc aggtccatca tccgacgggt cacccgcttg atttccaaaa
     6661 tcggtgcaac aatagtccga gcgtagatgt aggccaccaa aagggaaatc agaaaggagg
     6721 ccagcaaggt atagggaaga aactggagac tgatttgctc cgcttccttt tgtaaatcca
     6781 tggaagctag aaactggaga atcatagtac caccgtcttg cgttttcacc tcgcgctcct
     6841 caataaagag agaggttgtc tggcggtctg tgtccagagg aagactgtcc ttgacttcta
     6901 acttgtcctc ggtcatctca cctttgacgg tccccttgat atcactagtc tgggaataca
     6961 agtctaacac ttgctcgata ctctgcctat ctttcccttc tagggactgg gcaatggctg
     7021 ttgccttttg accaatggtt tcctgacgat gactcagata agtcgaagga aaaagaaaat
     7081 aaatagctaa atgaaggcag ataaccagaa cactaaatat cgagaaggta tagataaata
     7141 tctttgcaaa taaacctgtt cgtttcattt tcgctccaat ttataaccaa cattgcgcac
     7201 agtgaggata caatccaagt ctagcttttt ccgcaattcc ttgatataaa catcaataac
     7261 acggtcaaag ggaacctcat ctgtcgcttt ccagacggca tcgataatct gagatcgagt
     7321 caaggcccgg ccttcatttt tcactagata gtccagaatt tccaactctt tggcattgat
     7381 aggcacttct tgacctgcga ggcttgcact gtagctttca aagtccacct tggtatcctt
     7441 gtaagaaaag attcgtcctg tatcgtagta gcgcttgaaa atcgcgtcca ccctcacttt
     7501 taaaagggag agggagaaag gtttttccag atagccatct gccaaagagg caaaggcact
     7561 catcttgtat tcctcatctt gaaaagctgt caacatcaag acaggaacct gactggtttt
     7621 acgaatctca gctaggactt ctaagccgtt gagcttgggc atctggatat ccagtaaaac
     7681 cagggccacc tcatagctag aaaattgctc cagagcttcc tgaccgtccg ctgcctcaat
     7741 agtttcatag ccacaatccg tcaaataatc actgaccccc tcacggatca tctcttcatc
     7801 ttctacaatt aaaattttca tactttaact gctctctatt ttttattttt cttagaataa
     7861 atacctaccc tattttctat tatagtctct tgctggcctt ttgtctgcaa gcaactgacc
     7921 actagataaa acgttgtgaa attcctttct cataaattcc ataactttag tatattatat
     7981 ttaagcacta aagtacaaag aaagcaactg aaagcaatga ttttcaccac tgctttcgga
     8041 tttattttga attgttaaat agccattcct atccactatt cttgaataga aacacaagat
     8101 gcaatcttta ttctagactc attttttcaa atttattcac catccagcaa gagctctttt
     8161 ggttgttttc taaggagatt gcttgaagca agcgccataa cgagaaccac tagaaccaag
     8221 gcaaggacaa aaatgatgat aaagtctgat gtctgaatgg aaatgtctag gctcgacaag
     8281 gtcttgctaa agccatctac ttctgcacca ccaccaaggt tagaggcttg agccgcctta
     8341 ctagcctgtt tggcaacacc tgaagtcaca ttggcaagga cagtgtttcc aattgcacgg
     8401 gcagtgtaat tagctaggaa gtaagcagaa actagagcag ggatagcaat caagatagat
     8461 tcggtgatga attgacccaa gatacttgcc tgcttgaggc cgatagagag gagaattccc
     8521 acttccttgc gacgggcgtt gatccaaagg ctgagcaaga gggcaaggag gagaactgag
     8581 aagctcaagc taccccagaa gaggaggttg gccatcttgt acataccaga gatagattgc
     8641 tcaagagctg ggtagttaga ggagctcttg acgagtgtgt agctcttcca gttgatacca
     8701 ctgatgccat tcaactcttt cataacatca tccaagttct tgtctgctgt tacaaagaag
     8761 gttgcgtccc cataaatggc tgtgtcttct gtgtatccat aaagttttgc agcagtgtga
     8821 atgtctgtaa tagctgtgtt ttcgtaaagt tcttgtgagt aggttactgc tgacttatta
     8881 tgaccatcaa agagtccctt gattgtcact tcaactgttt ccttggctcc tttttcatta
     8941 tctgcatcgt agatattaga gtccagttta accttgtccc ctactttcca gccgtgtttg
     9001 gctgccaagt ccttgtgcaa gaggatttta tccttgtcgt cgttggttaa gtgctctcct
     9061 tcgactagtt tataagaacc agagacaaac ttgtcttctt tagaggagtc attgacacct
     9121 gtaatcatca agctacttcc aaaacgcttg gcacgatcag cagtgagatt cttcttggtt
     9181 tctggcgttt caatcaggtc atatccagtc aaatctccga tagcgttgat acgtttgaca
     9241 taagactcaa tggccttgtt ttcggtgatt tttttgatgt cttcaccctt gatattccca
     9301 gcaccacgag gcgttccttg gttgacgcga cgattgattt gcatggagaa gctattggtg
     9361 atatttttaa aggtctcctg agaagccttg gcagtagctc ccttgattga caagccgacc
     9421 aaactcaagc tcgccatgag gagaataatc aggaagatga caatcgattt gaaaaacttc
     9481 cttgtaacat aggcaaatgc gttgtgtaac atagattccc tttctagatt ttgttttaat
     9541 cattctatta aaataagctc aaattattta ctagtattgc gcgtttcagt cagtttctta
     9601 tcctttaatt caagtgtaat atctgacgct tgtgccactt ctttactgtg agttacgaca
     9661 atcacacatt tacctgtttt ctgggcaagt gatttgagta gttcgacaat atctccagca
     9721 gttttaggat ccagatttcc tgttggctca tcagctagaa taactggagc ttctgagacc
     9781 aaactgcgag caatggcaac acgttgctgt tgaccacctg ataactggag aacattccgc
     9841 ttgatctggc tttcatccaa accaagctca agaagtgtat tcttgcttgc ctttttgttg
     9901 accaatcgga tattttccag cggagaaaga taatctatca agttataatt ttgaaagacc
     9961 agggaaatat ggtgcatgcg atggtaagaa tagcccttct tacgaatatc ctctccttga
    10021 aaaaggatag aaccttcaac aggactatct agaccagcaa gtagggacaa gagtgtggat
    10081 tttcctgctc ctgactcccc aataatactg taaaattttc cgggttcaaa attataattg
    10141 atctgatata ggactgcttc agcagtattc ttataacggt aggtaacatc ttgtaattgt
    10201 aataaagtca tgatttctcc ttcttaacta atagatgata aaatttcttt cggtgatttt
    10261 ctaaataaga ataggaaaca aagggctaca gataagcaac taagcagaac tagaaaaaca
    10321 taggattctg caaaagataa gatgctagtt gataaactgc ttgctttggc tagtgtatct
    10381 tgtaagcttg cctgatctcc acttgctagt agagtttgga gtaggtaagt tgtgattgcg
    10441 tttcctgcaa caaatgctgg aagcaaagct ccaagagata ccaaaactac ctctaaacag
    10501 aattgtagga agatcgagct cttgcctttt ccaagtgcaa gtaaaatccc cacttcatag
    10561 acccgttctc tcaaccagag agacaaaacc agaattaagg ctccagctcc tgctatcaac
    10621 atcccataaa ggaagatggt caggaaggtt tggaaagttg caactgagtc tttgatttgt
    10681 tcaaaagcct tgttttcctt ttcgacttgg tagccttgat tttccaaggc caagttttct
    10741 acctgcttca tgagtccgtc catttcctta ggattttcta catagaagcg tgctgcactg
    10801 acttgagctt cactattgcc caaaagggtt tggctacttt catagtctgt aaagacttga
    10861 ttttcactga agtcagaaga caagcctgtg aatttctctt gttttttacc agaaaagatg
    10921 ccgataatct caaactctac tgtttgtcct tttccagatt cagactgacc agcatccaag
    10981 ccaatcttgt catgaagcga aagaccgttc ttcttagcca attcttcgtg gataaggatt
    11041 ttcttggaat cccctttttg aaggtgtcgc ccttctttta gattgaaagc cgaactggta
    11101 aaggttacat ccttggatga atcctcaaga gccgttaagc taaccaagtt attgtctgca
    11161 gctgataaat catcacgctc cacgctctgc tcgccagtca ctgcttcctt gtcttttagt
    11221 tttgcgaccg tctcaagttc aggagagaca ttttccagcc ccttaatctt gcttacagat
    11281 gctaggtctg acaacttgaa tgtctgacca ttctctatct tcttaataga aaaagatgta
    11341 ttgagtgatt tataaagatt gctttctact gttttgttgg acttcatcag agtcaaacag
    11401 gctgaaattc cggccaataa gaccaataaa atcagaaata aaataaaact tctcagtcgc
    11461 tttctgctga cataagccca agatctttgg attggattca tttgtcacct ccatatttgt
    11521 aagactatta taaaacccaa atatgaaatg tttatgaaat acgaaaaaaa aatatcgagt
    11581 aggggataat ctctagcccc tctcacacca ccatacgtgc cgttcggcat acggcggttc
    11641 aactaacttt taacgcatgt cgttcaaggt aataatccaa acacgaaacc agtccacgtt
    11701 tttcaaggac tggttttgat atagcacgtt taagtaccga cttctgagct actatagtag
    11761 attgaaacta gaatagtaca cctctacttc taaaatattg ttagaaatcg atttgacttc
    11821 cctgatcgat tcgtcctatt cttatttcat tttactacaa ttgatagtgg tcgccccagc
    11881 cagatacctt atctgctatc catttaggaa cccctaactt aagcaatccc cataatcgtc
    11941 tcgatttctt cttccattgc ttccagataa tcactcgtag gcgagtacgc aagcgctcat
    12001 ctatgctagt gactatactt ttcatattta taattcattc ctttcgtttc actcaaggca
    12061 caacacagaa tgaaaaagtg ttgtgatctt tattttgttt tataataata gtgagaaagc
    12121 ctatcactac tacaaatcac ggggaggtga ataagtgagt ggtacagcca ctacctcgca
    12181 tattttgtca catcatttaa cggtacataa taagttgtac catctgaata agttgctaca
    12241 atatcatttg catgctctcc ttcaccttta gcaaaggttg gagctcctgc tggatgattt
    12301 ttatttgcct ctttcaattt ttcaataatg gcattttttt ctgtatcttt tatattatca
    12361 ggatttttca ctaagatttt gtctggatat gtcggtttag cagaaacaat ttttactgtt
    12421 atttcttttt tattcgaagc acttgtccag tttccagcat tatctttagc atttaatttt
    12481 acagtaattc ctgaactagg aacttcagta gcaggttgat tatcaacatt attcaacttt
    12541 aatttcaaaa gagctgttgc atcagacgtt ttatcaatcg ttatatataa tgatgaattg
    12601 ttattataac cagtttcttc atatttagct gtttgtgagc tacttgaaac agaactgaaa
    12661 ttatgcccac tacctccctg attatcttca atgcttacgt ctaaatgaac ttccccacta
    12721 ttatttggct tagcaacaac tgttatagta aaataacata aaatttgcat aaatagatta
    12781 gggaaatcaa agcagcttct aggaatgttt tagcagtcac agtgtacttt cccagcatca
    12841 agccactata actctgcaca taaaaatgga gaagatggca atcctcttct ccaaatatta
    12901 acttctttac aaaccaacta tagttgacaa agaacctaaa atcaattgat aacacaaggt
    12961 caggtcggtc aactctttca actgaagccc tgtcaactct tcccatttat caatcttgta
    13021 ttggagagaa ttgcggtgca gatagagttg ctgggctgtt ttagtgagaa cagcactatt
    13081 ttcccaaaga gagagaatga tttcctgaat ctgatcttga tccaaaatca tctggtgtag
    13141 acattcttta attggcttca agtccacgag tctttctccc atactccaaa gatagagctg
    13201 agaaaaagta tgaacacctt ggtgaccctg acgccaccat gtcttgaaca aatcccgctc
    13261 agctttgatt aagtctgata gggcttgatg tcctgtctga gaccaaacct gacccaacat
    13321 gatagaaaga cgaagtccaa agtcatactc aaccgcttca atcatatcac ttaaaatatc
    13381 tgttacagaa gtgtatttgt cttgttgaag cacgaaaaca taatcctgag ctccgacctg
    13441 tagcactgtc tgacaattcg gaaaaagagt ccgcatcata tctagccaag aagccagatt
    13501 ttcctgctga aaataagaaa gatggcaata aaccaactga atctttttaa aaacttgcgg
    13561 tgcctgtccc ttgccctcaa ccagatagga ataccaaggg tttagcgaac gagcctgctc
    13621 ctgctgggtc aaaagggcaa ccaactgctt ttcacgctcg ctgagcccag cttcctccag
    13681 caaaatccac tgctgagaag ctaaagggag cgtgagatag ccctctttct ctactggttg
    13741 gtctgaaatc cgagcatcag gaaaccagtc ttgtagttct ttttccctca tgttctagcc
    13801 ctccactttt tggatgcacc atgaaaccaa actctcaaga cgttccagat tctcagtcat
    13861 atggagatag cccataaccg cttcaaatcc cgtggacata cgataagtca cgacatctgc
    13921 atttttagcc tttgtgtggc tattggtatt gcggccacgt ttgtagattt cttcttcttt
    13981 ttccgttagg acctgctcct ccaacatgag agcaatcagg cgagcctgag ccttggctga
    14041 cacatacttg gttgcttctt gatggagttt attgggtttg gtcatacctt tgaggatgag
    14101 gtgacggcga atatacatag aatacaccgc atccccctca aaggctagcg caatcccgtt
    14161 aatgagattg acatcaatca cgtgtccacc tcactccatc cttggtatca aggagcttaa
    14221 ttccttgagt aaccaattgg tcacggattt ggtctgctgt cgcaaagtca cgattggcac
    14281 gcgcctcttg gcgtttttga atcaagtctt caatctctgc atccaaaact tcctcaacaa
    14341 agacaattcc aaaaatttct aacatatctg caagagcttg cttgacactt gcatcatagt
    14401 tccctgagtt gatccatttg gccatttcaa agacaactgt gataccgttg gcagcattaa
    14461 aatcttcatc catagctgct acaaacttat ctttaaagtt ttgtaactct tgggcatcca
    14521 catttcctgt aaatggttgt tcgtaagtat tcttgagata cttgagattg gtctcggcat
    14581 cgcgaactgc cttttccgtg aagttgatag gcttacggta gtgctgggtc gcaaagaaga
    14641 aacgaagtac ttgcccatca agagttttaa gggcatcgtg taccgtaatg aagttaccca
    14701 aggacttaga cattttgaca ttgtcgatat tgacaaagcc attgtgcatc cagtagttag
    14761 caaaagcctt gcctgtttta gcttcagact gggcaatttc attggtgtgg tgtggaaact
    14821 ctaggtcagc tccaccaccg tggatatcaa tggtatcacc taaaatctct gtcgacatga
    14881 ctgaacactc aatatgccaa cccggacgtc caggtcccca aggactatcc caagaaatct
    14941 cacctggttt ggaagatttc catagagcaa agtctacagg attttcctta cgagccgttt
    15001 cttcatcggt acgacctgaa gcacctagct ccaaatcttc caaggtttta ttagccaatt
    15061 tagcatagtt gtgggatttt tctacacgga aatagacatc cccttgactc tcataggcaa
    15121 agcctttctc gatcaagtct tccacaaaac ggatgatgtc tgccataaac tccactacac
    15181 gcggatggcg agtcgcaggt ttcacgccca atgccgtcac atcctcacga aaggcagcga
    15241 tgtacttatc cgcaacctcc tgaggcgtga taccttcttc cctggcacgg ttgataatct
    15301 tatcatccac atctgtaaaa ttggaaatat aggcaacctt atacccacgg tactcaaaat
    15361 agcgacgaat ggtatcaaaa gctaccgtcg aacgggcgtt tcctacgtgg atatagttgt
    15421 acaccgttgg cccacaaaca tacatcttga tcttgccgtc ctcaatcggg acaaattctc
    15481 gcaaatcacg agacatggtg tcatagattt taatcataaa tcataatcag gaaagctgaa
    15541 atccaagaac aattagtttc atcactaaaa gttcaagtaa atttcagtcc gaatatctct
    15601 acacttcgga atcccttgct cctttctcat tcagataaac cacctgagtc tgtttgacaa
    15661 agccaatttt ttcatacaaa cgtttggcac ctacattgct atcttccact gcaatctgaa
    15721 attccttgtc attttgctca attagttggt tgacgaggga ttttgctaag tagcttccat
    15781 agccttttcc acgttcaggt tccaatattg ctaaaccgta gaggtaattc gtattagtcg
    15841 ataaatcaac cgtacaagtt ccaataacct gaccagcttt taataaaata tatagtcggc
    15901 tttctggatc tttcagagct tcagcgacat atctatccac aacttctctc gattcatgtt
    15961 cctctgaaaa tgcctgaaat tttaattgac taatttgatc ctgatacgaa ctatctgcta
    16021 acaaaacttc aagatgggaa acatttgcta acggataagg tcttctatcc ttacctaacc
    16081 aagtttctgt ctcttcatcc tcgattagtc cccagttact ggcaaagtca ggatgattct
    16141 ctaaaaaaat acgttctgtc tgaaaagtga ctgaccgaat ggggaaagaa gctgtttctc
    16201 tctcaaaact agtaaacaat gcacgcgcaa tcccctgacg gcgatgacct ggatgaacca
    16261 gtatcgtcac ttctacatct tggtcatctg catagacagt taataaacca acaagttcgc
    16321 ctttttcata ataaaggaaa aaggcgggca tgtttgggtc aaaattaagc atgttagaga
    16381 gatagggatc gcgataggta ccgtcatagt tttggcaaca gttaattact tttttcgcct
    16441 cagatagctc ctcttggctt aacttgtttc ttgcttgaat catataggta tcctctacaa
    16501 accagacgat ctgtgactgg catctttagc ctgctcgagt ttattgacat aatactctcg
    16561 tttttcttcg acttcgtgaa tgacaggctc atctttctta ccatgaagac ggacaatctt
    16621 ggccggaata ccgacaaccg tcacgtcact aggtacatct gctacgacaa ctgctgcagc
    16681 accgaccttg gcattttcac caatttccac aggcccgata acttgggcat gggctgatat
    16741 gagggctccc tttcgtacag tcggatggcg tttgccacag tctttccctg ttcccccgag
    16801 agtcactccg tgatagagaa gaacgccttt ttcaacaatc gctgtctctc caatcaccag
    16861 accagaacca tggtcaataa aaacacctga atcaatctgg gctcctggat gaatctcaat
    16921 ctgagtccaa aagcgccaaa actgactgta catacgagct aatagtttga agccgtgctt
    16981 ccagagaaaa tgcgagagac ggtgggccgc caaggccttg acacctggat aagtcagcaa
    17041 aacctccaaa gtggtgcggg ccgctggatc attttctttt acaatatcaa tggtttcgcg
    17101 ccaccacccc atacatttct ccttttctta ttctgaatct tttgatgttt ctgtaaattc
    17161 tttcttaggt ttgtaatcct tttgatgacg tgggcggtga gggcgctcag acttttcacc
    17221 tttttcatca tgctcaggtt ttggcggacg aggtagaaga gccttcatag aggcatcgat
    17281 acggcctttt tcatcaattt tgataacctt aacatcaact tcatccccga tttctaccaa
    17341 atcctctaca cgattggtac gagtccaagc catctcagag atatgaacaa gggcatctgt
    17401 cttatcaaag aggttaacaa aggcaccaaa tttctcgata cgaacgactt tagcacggta
    17461 aacttcatcc actttggctt cacgaaccaa accagcaata atttctttgg cacggttaat
    17521 agcatcttgg tcactagagt agatagacac atttccttct tcgtctatat caatcttaac
    17581 acctgtttca gcgataatct tgtcgatggt ttctccaccc ttaccgatga caatcttaat
    17641 cttgtccaca tcaatcttga tcgtatcaat tttcggagca gttggagcca attctggacg
    17701 aacttctgga atggttgctt caatgacatc aaggatttca aaacgcgctt tcttggcttg
    17761 agcaagagcc tccgtcaaga tttctgcagt aatcccttga atcttgatat ccatttgaag
    17821 ggctgtaatc ccatcacgag tacctgcaac cttgaagtcc atatctccaa agtgatcttc
    17881 caaaccttgg atatctgtca atactgtgta gttatttcca tctgagataa gtcccatagc
    17941 aataccagct actggcgcct tgattggcac accaccagcc ataagggcaa gagttcccgc
    18001 acagatagaa gcttgagatg aagaaccgtt tgattccaaa acttctgcta ctagacggat
    18061 agcgtatggg aattcttcca agcttggcaa gacttgagca agagcacgct caccaagggc
    18121 accgtgaccg atttcacgac gacctggcgc accgtaacga cctgtttccc ctacagaata
    18181 ttgagggaag ttatagtggt gcataaagcg tttcttgtac tctggatcca aaccatcaat
    18241 gatttgagtt tctcccatcg gagccaaggt caagactgaa agagcttgag tttgcccacg
    18301 agtaaagaga cctgaaccat gtacacgagg aaggaagtca acaaccgcat ccaaaggacg
    18361 gatttcatcg accttacgac catcaggacg caccttgtct tctgtaatta aacgtcgcac
    18421 ttctgcgtgt tccatttgtt ccaagatttc agccacatca cgcataatac ggtcaaattc
    18481 ttcgtggtcc gcatattttt cttcgtaaac ggcagtcact tggtctttca ctacttgagt
    18541 cgcagcttca cgggccaatt tctcttctac ttgaactgcc ttttggaggt cactgttgta
    18601 ggctgcaatg atttcagctt gcaattcagc atccacgtga agcaattcca cttctgcttt
    18661 ttctttaccg acagcagcaa cgatttcttc ttggaaggca atcaattctt tgacagcttc
    18721 gtgcccttta agaagcgctt ccaacatgat ttcttctgac aattctttgg caccagactc
    18781 taccatgttg atagcgtgct tggttccagc tactgtcaat tcaagaagag attgctctgc
    18841 ttgttcttga cttgggttga tgatgatttg gccatctaca tatcccactt gtaccccagc
    18901 aattggtccg tcaaatggaa tatctgaaat agacagtgcc aaagatgaac caaacatagc
    18961 agccattggt gcagatgcat tttcatcata agaaagcact gtattgatga cttggacttc
    19021 attacggaaa ccttccgcaa acataggacg aatcggacgg tcaatcaaac gcgctgtcaa
    19081 ggtcgcatct gttgaaggac gtccttcacg tttcataaag ccaccaggaa acttcccagc
    19141 cgcatacatt ttttcttcgt agttgacttg gagtgggaag aaatccccag ttgccatttt
    19201 cttagacata acggcagcag tcaagacagt tgactcaccg taacgtacga caacagatcc
    19261 atttgcttgc ttagcaacct gaccagtctc tacaattaac tcacgacccg caaaagtcgt
    19321 ttgaaacact tgttttgcca ttttaatccc ctttggattg atgaaattat acgccttgcc
    19381 tacaaagatc aagataccaa ggacgtcaaa agcaaagtaa aaataggaaa ctgacgaagt
    19441 cttcgatgaa gacaagacag tttatctttt ttacacagct tttcggccgt gttcaattac
    19501 acaagatatt ttggacggtt cggcttgccg aacatttctg tagaaaaata ggaaggtgac
    19561 gtcgcactcg acgagtgcta ggaagcttat cttttttcct aagaaatgag accaaaattc
    19621 aattactcaa gatactaagc cgtcaagcaa ctcaaaggaa aataggaaat cgaacgacgg
    19681 agcgactgct cctagggaga tttatctttt tccacagagt tgtaggcaag ttcagttttc
    19741 aagatacatc attagaaagg tttaatacta aagtatctaa agctttcacg ctaatcgcta
    19801 tcgggcgatt agctaaatgc tttactaact ctctcgtcaa ataacatcga tttgactcac
    19861 tcgtgtcgtt aaatcttaca gtttaaatgc attgtattat ttaatacctt catctttgta
    19921 tcaagtacgt acagaattta ttttatcata tttttcttaa aaagtgaggt ctttaccatt
    19981 aaaaaggaac cattcccctc acctgagaag aatggtttgc ttttattatc ctagagactg
    20041 gtgattaaac aaggcatggg ttgcttgatg gatgtatttt gctgtatcag cattattcat
    20101 cgtatagaga tgcacaccgg caatatcctg agttaccaag tccacgattt ggtccactgc
    20161 ataggcaagt cctgctgctc tgagcgactc agggtcatgc tcatacttgt ctaagatggc
    20221 tttaaatttg cgtggaagat ggatattctc acaagtcttc aagagtcgga gagcctgatt
    20281 tcgattcaga attggcataa ttcctgcatg aatgggaaca tcaatcccag ccaagatgca
    20341 cttgtcctga aaatcataga agcgctcatt gtcaaagaag agctgagtta cgaggctcga
    20401 acagcctgca tccactttct tcttaagatt ttgaatatct gaaatctgat ttggcgaatc
    20461 tggatgccct tctggatagc aagctccaat aatatcaaag tgaggggctc gttccttgat
    20521 aagctcaatc aagtcggttg catagcggaa atccttttgt ggttccacgt ctggaataat
    20581 atccccacga agagccaaga ttttctgcac cccaactttg tccaagtcag caatagtttc
    20641 agcaaccttg tccttagtta gataaatagc tggcaagtgg gcaatggtcg gaatcgccaa
    20701 atcattttgg ataaagtcag ccaaacgaac cgtcgtttcc ttgatattaa atttattatt
    20761 gctggcagtt acactgataa aatggggagc caactcctgc atatcctgca aggctgaaat
    20821 aatgttatca ttacccacgg ctgggtttgg agggaacact tcaaatgaga gtgacggtgt
    20881 ttggcgtgac atatgtaata accttttcta gttgatttct ttttgaacaa ccactgtatg
    20941 gagagaaatc caatcttaca atttctcacg cgcagcttta gctgcttcaa caaggcggat
    21001 caagctttct tttgtttctg ggataccacg tgttttcaaa ccacagtcag ggttgatcca
    21061 aactttcttg cttggcactt tagcaaggat gacttcgatt gtgttgtcga tttcgccttc
    21121 atttggtaca cgaggtgagt ggatatcgta aaccccaggt cccacttctg tttggaagtt
    21181 tttcgctttg agttcgtcca agatttcaag gtttgaacgg ctagcttcaa aggaaataac
    21241 gtctgcatcc atgttatcga tagctgggat gatatctgta aattctgagt aacacatgtg
    21301 agtgtggatt tgtgtgtctg gcgctactgt tgagtgtacc aagcggaagg caggaatagc
    21361 ccagtcaagg tagtcttcgt accagtcgct acggcggagt ggcaattttt cacgaagagc
    21421 agcctcgtcg atttggatga ttttcacacc agcagcttca aggtcaagta cttcatcctt
    21481 gatagcaagg gcgatttgga gagttgaatc cttgatagag atgtcttcac gtgggaatga
    21541 ccagttaagg atggtaacag gtccagtcaa cataccttta acaggtttgt ttgtacgact
    21601 ttgtgcatag ctagaccatt taacagtgat agggttaaga cgagtgacat caccccagat
    21661 gattggtggt tttaccccac gcataccgta tgattgtacc catccatttt tagagaagag
    21721 gtatcctgac aagttttgac cgaagtactc aaccatgtca ttacgctcaa attcaccgtg
    21781 aacaaggaca tcaaagtcaa tatcttcttg ccacttgatc cattcgtcaa tcgtttcagc
    21841 aaggaaagcg tcgtactctt tttgagacaa ttcaccttta cggtaagcca aacgtttggc
    21901 acgaacttct tttgtttgag ggaatgaacc aatcgttgtt gttggaagag ctggaagttt
    21961 gaaagcttct tcttggatag cttcacgttc tgcaaaggct ggcaaacgag tgtagtctgc
    22021 gtctgtcaag ccagcgatac gcgcacgaag ttcagcattt tcaccaacac gctcagtcgc
    22081 aaagagttct ttgttggctg caagagcttc tgaaccttga ccatttcgga tagcatccaa
    22141 gtcacggatt tcatccaatt tttcaactgc aaaggcaaag tggttcaaga gtgctggttc
    22201 aaattcttca ttagcagttg taaatggcac atgaagaagt gagcaagagc ttgtcaaaac
    22261 gatgttttca gctgggattt gctcaagaac agtcaagctc ttttcgtagt tgttgcgcca
    22321 gatgttttta ccattgacaa tacctacata gagagtcttg tcagctggga agccaccttt
    22381 aacgagttca agagttttct taccttcaac aaagtcaaga ccgatagcat ctactggtaa
    22441 gtttacaagg tcagcgtata cgtcacgaac atcaccgaaa taagtttgaa gcaagacttc
    22501 aagacctttt ttgtcagcca agagtttgtt gtaaaggttc aagaaaagag ctttttcttc
    22561 agctgtcaag tcttttacaa gagccgcttc atccaattgg atgcgagtcg caccaagttc
    22621 agccaattta gcaaaaactt cttggtaagc agacactaag ctatctacga agtcgtctgc
    22681 tttcacgcct tcttcaaagt ctgacaattg aaggaaagtg aagggaccta caagaacagg
    22741 acgagtgttc aatccaagtt ctttggcttc ttggaactca tcgaaaatct tgtgaccagc
    22801 caattttact tgagtgtctt tttcaaattt aggaacgatg tagtggtagt tagtgttgaa
    22861 ccatttcttc attggaaggg cgcgaacgtc ccctttttct ccctggtaac cacgtcccaa
    22921 agcgaagtag cgctcaaggt cagacaagtc caagttttga acggatgcag gcaccacgtt
    22981 gaaaaggaaa gccgcatcta ggaagttatc atagtgagaa aagtcatttg atggaatttc
    23041 agtgatgcct ttttctttga caatgttcca gtgtttagca cgcaagtctt ttgctgctgc
    23101 taaaagttct tcttctgaga tttcttttct aaagtatttt tcagttgtaa attttaattc
    23161 acggaattcg cccaaacgag ggaaaccgac gattgtagtt gacatgatgt gtcctccaaa
    23221 atttgttgtt gaaactatct taacagaaaa gaaagcgtct gtataattgt aaaaaattag
    23281 ggtttgatat agtttgaaac tatatatctg tttcggacaa aagaaaaaga cttgaagcaa
    23341 acgtctcaaa tcccttttat aacttgtttt tagctgtatt ttagttagaa tgctcaaacg
    23401 cgttattagt aattcttata agtgactatg gcttgttatt agaaaagact ataactgatt
    23461 ctagtcaact tttcccctgt tcaagtggaa cgactgctag tgtctttcct agactagcta
    23521 ggacttttaa gactgtgtcc aactgtggac tagtcttacc tgtctccatc ctagctataa
    23581 caggctgact cactccactg acttcctcta gctttttctg actgattcct tgcttatttc
    23641 tggcttcaat caactcgctc atgatagcta ctcgcatatc actttcaagg atttcctcct
    23701 tgctaaagag ctcagataga catccttcca attactccca atagcactat tcttcatcac
    23761 ttacccctct ttcttttaga tctatgtatt ttttgaaaaa atgagcgaat tatgattcga
    23821 tagattgatc agcgggttta aaattgggac taacttgttt cttaacagat tttccttttc
    23881 taggataaag caaatcctgt ttgcttaacc ccaattttcc acgatgaatc caatagtaaa
    23941 tggttgaaat tcccacgtta acccctttag ccataaccat catttcaggc gaaaattttt
    24001 ggttatgtat agtggagaat cttttccttt agttccttgg tcaagcttga tttcttgacc
    24061 gagcgcttgc gattgttttc ataagactgt tgagcgtagt cggcagaata aacctctttg
    24121 aagcgccctt ttccaagaca ttttcggact gtcccacgct tgatttcagt gtggatagtt
    24181 tgaggagctt ttccaagtag agaggcaact tctctatttg attttccttc ttttttccat
    24241 ctttcgatta agcgacggct atcgattgtc aaatgtttac cttttgtagt ataattgtct
    24301 tgcatctctg tgccttttaa tcatttcaat cttaaattgg acttttttta cttgggttgt
    24361 acttaatcta taaggaagac aataaaaaga atatcaatca agtaaagtca caaagtcaca
    24421 ttagctccga gcaaccattg caaattgagg tactcacaca atgattaaaa catttctctc
    24481 tgccctttcg gtcattctct tttctatccc tatcataact tattcttttt tcccatcttc
    24541 taatcttaac atttggctat ctacccaacc tatcttggca cagatttatg ccttcccctt
    24601 agctactgca actatggctg ctattttaag tttcttattt ttttcctatc tttttacaag
    24661 aaaaataaac aaatacggtt ttactctggc attttgctct tactatcgct catattacta
    24721 ttattcggaa cagataaaac cctttcttct gcatcaaata agactaaaac cttaaaatta
    24781 gtaacttgga acgtcgctaa tcaaatagaa gcacaacata ttgagcgaat ttttagccat
    24841 tttgacgccg atatggctat attccctgaa ctagctacca atatcagagg tgagcaagaa
    24901 aaccagagaa tcaaactatt gtttcatcaa gttggacttt ctatggccaa ctatgatatt
    24961 ttcacttctc cacctaccaa tagtggaata gctcctgtga ctgtgattgt caagaaaagt
    25021 tatggtttct atacagaagc taaaactttt catacaacac ggttcgggac aattgtatta
    25081 cattcgagaa aacaaaatat accagatatc attgccttgc atactgctcc tcctctgcca
    25141 ggtttaatgg aaatctggaa gcaagactta aacatcattc ataatcaatt ggcttcaaaa
    25201 tatccaaagg ctattattgc aggtgatttt aatgcaacta tgcgtcatgg agcacttgca
    25261 aaaataagct ctcataggga cgcattgaat gcacttccac cttttgaaag aggaacttgg
    25321 aatagccaaa gtccaaaact ttttaatgca acaatagatc atattttatt gcctaaaaac
    25381 cactactatg ttaaagattt agacattgta agttttcaaa actctgatca tagatgtatt
    25441 tttacagaaa tcacatttta attattttat ataaaatcac ccctctagtg ttcataaact
    25501 agagggggaa tttgtatcct actatcgttt aacgcacttc tgcattgact ttttcttcga
    25561 gagacgcttg gattttttcc atgtagcgtg cgacttcttc gtccgttaag ctgtcttctg
    25621 gattttggaa ggtcaagcta taagccattg acttcatacc aagtcccagt ttttcacctg
    25681 agaagacgtc aaagagtttg atatctgtca aacgtttcac gccggcagct tggatagcat
    25741 ctacaacttc ttggtgagtc acttctgcct tgaggagaag ggcaacgtca cggctgactg
    25801 ctgggaattt ggtgatttcc acaaatggaa cagcaggttg gagcgcccct tcgatggctg
    25861 aaaggttaag ctcagctaca tacgtttctg gaatatcgta agccttggca gtgactggat
    25921 gcacttggcc aaggaaacca agaacttggt caccgagtga aatcacggct gtacgacctg
    25981 gatgaaggct agcgatttca gatgttgctg tataggttac ttggagtccc aaacgagtaa
    26041 atagggcttc aaggattccc ttagcataga agaaatcaac tggaactgct gctgtttgga
    26101 aatctttttc agcaaccaag cctgtcaagg caaaggcaaa gctgttgatc tcatttggaa
    26161 gttcttcttt tggattacct gtttgttcaa agacttttcc aatctcataa agggccaagt
    26221 ttttattctt acgagccacg ttgtaggcaa cggtatcaag gatccctgaa atcatatttt
    26281 gacggaggac tgaacgatcc acagtcattg gccacatgag ttcagtaagg ttacttggtt
    26341 gagctgtgaa ctcaactgct ttttcaggag ttgtcagagc ataggtgata atttctgtca
    26401 aacctgctcc ttcagcaatg gtacgaactt gacggcggag tttttgtgtc acagtcaatt
    26461 caccagctgt accatcgtct tttggaaggc tggttggcaa gcgatcatat ccatagatac
    26521 gagcgatttc ttcaaagaga tcagcttcga ttgtgatatc ccaacgacga cgtgggactc
    26581 tgactgtaaa gctgtctgca tttccagaaa gaccaaagcc aagacgacgg aagacgtctt
    26641 ctacatcagc ataagacagc tcagttccga ggacacggtt aacatcagca agggttgaag
    26701 aaacttctac atctgaagta tcaagctcac ccgctgaaac gatgccctta cgcaccgtcg
    26761 cacctgcaag ttccgcaatc aggctagctg ccgcatcaag ggcttcatta actgttgcca
    26821 cattaattcc tttttcaaag cgagaagatg actcagaacg aaggttcagg cgaccacttg
    26881 tcttacggat agatttgcca ttgaaaacag cagcttcaag gacaacacga ctagattttt
    26941 cagagatttc tgttgcttga ccacccatga cacctgcaag ggctactggc ttgtctgcga
    27001 cagtgatgac taggtcattc acgtccaagt cacgttcttc accgtccaag gtcaccaatt
    27061 tttcaccagc acgcgcttca cgcacacgga tgtcagtccc ttcaaagtta tccaagtcaa
    27121 aggcatgcat tggttgacca aaatagagca ggatgtagtt ggtcacgtcc actacgttat
    27181 tgatgggacg gattccttcg ttcatgagaa ggttttgcaa ccattgtgga cttggtgcga
    27241 tggtcacatt gtccaagata cgagctgcat agtaaggcgc cttgtctgtc tcaatgatga
    27301 cagaaagggc atctgccgca gcttcattag tttctgttag agtaaattct ttaaagttga
    27361 ctgccttgtc atagatggct gccacttcgt gagccactcc acacatagaa agggcatctg
    27421 cacggtttgg tgtgatggaa agttcgatga tttcatcatc caagtctagg taagaaaaga
    27481 cttcctcacc tggcacggca tcctcaggca agatttggat gccatctgcg aattccttag
    27541 gcacaactga gtcagaaatc cccaattcac caagtgaaca gatcattcca agtgactcca
    27601 aaccacggat ttttcctttt ttgattttgt agttatcagc gatacgagct cctggaagag
    27661 ccaccatgac cttgatccca gcacgcacat ttggggcacc acaaacgatc tgacgggctt
    27721 cttcttcgcc aacgttaacc tgacaaacat ggaggtgagt ctctggcaca tcttcgcaag
    27781 acaagacctc accgacaaca atttttgaga gatcagcagc tggtgattcg acactctcta
    27841 cctcgatccc tgtggttgac attttttcag ccaactcctg tgatggcaca tcaatgtcca
    27901 ccaattcttt taaccattta taagatacaa gcataattta gttctccaga atgacagttg
    27961 tcactctagt tcttttcctt tcctatcatt tcaatagaag aatcctcttc ttaccttaat
    28021 ttctttctca gtaaccaatc cgtatctact ttttgaccaa ccataaaatg atgttggcta
    28081 aatttttcaa aaccatatcg attataaaac gcttgagctt ttgtattatg ctcccaaaca
    28141 cctaaccaag cccaagaaaa actatttttt gtagcaagtt caagtgcgaa ttcaaacagt
    28201 tgcttaccta gtccaaatcc ttggaatttt tgtagcacat agaggcgttg aatttcaaaa
    28261 gcgtcctcta attctctctc agtttgagca cttccccagt tgactttgag aaaaccagct
    28321 atctcctcct catgcataat gaaataggtt tcagagtcag gatttcccaa ctcagttgac
    28381 acagttttca gactataagc ctcttcaaag tattcctgta actgctcttc cgtattatca
    28441 tacgcaaagg tttcacgaaa ggtttgtttg gcaattttag ccaacacctc aacatctgcc
    28501 atttctactt ttctaatcat tatttaaact gttctgagaa gcggacatct ccttggtaga
    28561 atccacggat atcgttgatt ccataacgga gcatagctac acgctcttgt ccaagaccaa
    28621 aggcaaagcc agagtataca gtcgcatcga taccactcat ttcaaggaca cgtgggtgaa
    28681 ccataccggc ccccataatt tcgatccaac ctgttttctt acatacatta cagccttctc
    28741 caccacactt gaagcaagaa acatccacct caacagatgg ctctgtgaat gggaagtaag
    28801 atggacgcaa acgaatttga cgctcttcac caaacatttt ttggacaatc aactgaagcg
    28861 ttccttgaag atcagccata gagatatttt tcccaactac caagccttcg atttggtgga
    28921 attggtgact gtgggtcgca tcgtccgtat cgcgacggaa gacacgccct ggcgagatca
    28981 tcttcaaagg acctttagaa aaatcatggg catccatagc acgcgcctga actggagacg
    29041 tgtgggtacg gagcaagatt tcttcagtga tatagaaagt atcctgcata tcacgagctg
    29101 ggtggtcttt tggaaggttc atacgttcaa agttatagta gtcttgctcc acttcaaaac
    29161 catccacgac ttgataaccc ataccgatga agatatcttc gatttcttca ctggtttgtg
    29221 tcaaaacgtg acggtgacca gtcgcaactg gacgacctgg aagcgtcaca tcgatactct
    29281 cgctagccag ttgagccgcg actttctttt cttccaagag cttagctgtt tcttcaaaag
    29341 cagcagtcaa gacatcacga gcttcattga cgtgtttccc gatgattgga cgcatctcag
    29401 cagaaacatc tttcatccct ttgaggattt cagtgagcga acccttttta ccaaggacag
    29461 agacacgcaa atcttgcatc tctttttcat ttccagcagt aatctgcttc aagctagcca
    29521 gcgtttcttc gcgaagcgct tttaattgtt cttcaatagt tgacatattt cctccatcag
    29581 tctctcgtag ataaaaagaa aaccacatgc caaaaactcc actcggagcg ttgacacgcg
    29641 gtaccatccg ttttcatctg acaagtcaga ccttcatttc taaatccatg cgcaagtgaa
    29701 ttcacccagc tttcatatag agagcttgca gtcacggctc tcctccctga tatacttccc
    29761 ttgggctact agtctttcag attcctattc aattactact tagtttatca gatttttacc
    29821 attcttgcaa gacctatctt acttctgctt gtcagcttat tcttatctaa ctttatagcg
    29881 gattgaaata agatgaaaac aaatcgattg ggaaagtaaa attaatttct ataaatgttt
    29941 tagcaattat ttcgtactat tttagattca gtctactata tataaccaat ctatcctaag
    30001 ttaactattt ataatcggct ctctgtaaca aaattaaatt aattaatccc cctataaaat
    30061 aaagaggttt agaatttgat gtcttccaaa cctctttatt tcatatttaa tgaaatgcca
    30121 ccttaaccgt gataatagct agtcatcaat aaaaaactat ttgaataagg attctccatt
    30181 tgattcaatc actcctttat accaagtaaa ggacattttc ttatatcgat tgaatgtgcc
    30241 atttccatca tcatttcgat caacataaat gagaccgtac ctcttagaaa gctgtgcagt
    30301 ggacatagaa acacagtcaa tacatcccca agacgtatag cccataattt caacaccatc
    30361 ctgtagagct tcagcaactt gcaataaatg ttctttcata tactgaattc tataatcatc
    30421 ttggacggtt aagttattaa gttcatcttt tattagttga tctttagcac ctaatccatt
    30481 ttctactata aataatggga tttgataacg gtcataatat ctatttaaaa ttatacgtag
    30541 tccaattgga tcaatttgcc atccccactc agaagactct aaataaggat ttactaaacc
    30601 accaataata ttcccttctc ctgaattata ctgtgttgga agagcagatt gagtcacact
    30661 catgtaatag ctaaaggata aaaaatctac agtataattt tttaataact ctgcatcttc
    30721 agctgcaaac tctatgttaa tgtcatgttc cttaaaatat ctttttgcat aattcggata
    30781 ataacctcta acatgcacat ctgaaaatag ataatttaga ttctcatact catgagtcgc
    30841 ccatacatct tttggatttg gagtcattgg ataagctggc atagctaata ccatacaccc
    30901 caccttaaac tctgaattaa tctcacgagc aatttttgta accaaactcg aggcgactaa
    30961 ttcatgatgt atagcttgat ataattcttg tttcgaaaga ttctccttag gtatatctat
    31021 tcctccacta gtaaatggta attccaaaac agagtttact tcgttaaatg taagccaata
    31081 tttaacttta tctttatacc tttctaaaac tgttcgagca aatttttcat aaaaatgaat
    31141 cattctccta tcaacccatc catgatattt tcttgctaaa tataatggag tctcatagtg
    31201 tgaaagagtt acaagtggtt ctatcccgtg agcatgtagt tcatcaaaca attcatcata
    31261 atatttcaac ccagcttcgt taggttcttc ctcatctcct tttggaaaaa ttctactcca
    31321 tgcaatagaa gtacgaaaaa cattaaagcc catttcagaa aacaaggtta tatcttcctt
    31381 atatttatga taaaaatcaa tacctatcaa ttttaagtta tcttctgtag gattttctgt
    31441 tgcttctcct aatccacctt tgggtaacac atcctgaact gataagccct taccatcttc
    31501 attatatgct ccctctactt gattagctgc aacagctcca ccccaaagaa aatcatctgg
    31561 aaaaatggtc ataactttcc tccattataa tattaccagt aattccttag aatgctcgat
    31621 tgtctgatta ttaggtaata ctaatacatc tagaaaatca ttggtattcg ttacaattac
    31681 cggtgtaact gtttcgtagc ctttagtctt gattaaattc aagtccattt caaaaatcaa
    31741 ctgatttttg aaaactctgt ctccttcttc tacatgacta ataaaacctt gaccttttag
    31801 ctcaacagta tctaatccaa tatgaattag taactcaaca ccctcatcac tcttcaatcc
    31861 aattgcgtgc ttagtcggaa aaatatttgt aattttccca tcaaatggtg cataaacctt
    31921 accttcactt gggataatcg ctactccgtc tccaattagt ttatctgaaa atgttttatc
    31981 ctggacatcg cttaacggaa tgatttctcc tgatatagga gaaaatatca tttttttatt
    32041 tgaaactcca gctttaactt ctaaattgct agaactctct tcttcatcga ttccaaatat
    32101 ataagctaat acaaaggtaa taacaaccga aatgaccgcc acaattaaag catttacaat
    32161 atttgatggc acatcagaat aaataaattg aggcaacgct atcaaagatg ggacagcaaa
    32221 tagatacgct ttaacactag taagacctgc aaataatccc gctaatccac caccaatcat
    32281 agctgcataa agcggttttt tatattttaa agtcacacca tataatgcag gttcggtaat
    32341 ccctgcaagt aaggctgaga aacctgctgc aaaagcaatt tgttttgtat tattattttt
    32401 actctttaat gcaacagcca tcgaagcagc cccttgagct aagtttgacc ctaacattgc
    32461 tggaagaatt aatacgtctg gagtagcaat agatgccgcc aaaaaaatag gtgcaaaagc
    32521 ccaatgcatt ccagtcataa caataaatgg cataatagcg ccaagaatag ctaatgtaag
    32581 ccatccagct acaccataca tttgcccaac tagatttgat aatccttcac caacaattac
    32641 tccaataggt ccgactacaa ctaaggcaat acagcttgat actaataata ctagcgtagg
    32701 ttgcaaaaaa ctcttagtaa cagctggtgt taatttagca atcatttttt caatatattt
    32761 catcaaccaa accataataa gaattggaac gactgacgaa ccataactag ctggtgtcac
    32821 aggtacaaca aataaactaa gaggattccc tgattgcacc atttgaacaa aatttggatg
    32881 gagaagtaca cctgctacag acatagctaa tgtagatgtt acttttaatt tttgtgatgc
    32941 agaataagct aataacagcg gtaagaaata atatggagca tccccaaaaa atgtcaaaaa
    33001 agcaatagtc tgagaatctg attgcaatat accaagcatt ggtaaaataa ttaccaagac
    33061 tttcagcata cctcccccta acattgctgg aatgattgga gtcatggaac cagcgatata
    33121 ctcaatgatt ctttctaaaa tattcccttt gtgcccttga acaactgaat cggattcaaa
    33181 attgccaagt ttaacgaatt ctttataata attagctaca tcattaccaa gtataatttg
    33241 atattgtcca ttctttttca taatacctat tacacctggt atcttcttca catcatcatc
    33301 attgactaaa ttttcatctt ttaattctaa tcttaaacgt gttacacaat gggtaactct
    33361 attgacattt ttttcgcctc caattacatc gaggattttt tgtaccgtat ctttataact
    33421 catggtattc tcctattcta ttaatctaaa ttttttgtta agcgacgaat atgagccatc
    33481 aaataaacta attcactaga agtcagcaaa taattgtact ccgtttgtat aaacattgcc
    33541 acctgttcac cacattcata ttctctagga tatttatttt tcattaatgc taacaagtct
    33601 tcatcatcat cgtcacaata agttgtattt tcaaccaaac gatgggcaaa taattttata
    33661 tgagttatga atctataata atcaatagaa tcttggttaa actcagtaca atagaaatct
    33721 tgaa
//
LOCUS       DBERDK010000017        33437 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.017, whole genome shotgun sequence.
ACCESSION   DBERDK010000017 DBERDK010000000
VERSION     DBERDK010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 33437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33437
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.017"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(1..406)
                     /locus_tag="ACL73L_000781"
                     /pseudo
     CDS             complement(1..406)
                     /locus_tag="ACL73L_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187281.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
     gene            complement(780..929)
                     /locus_tag="ACL73L_000782"
     CDS             complement(780..929)
                     /locus_tag="ACL73L_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope"
                     /protein_id="HGS0771789.1"
                     /translation="MEKFNFKNNIGQENKLLQIEIYKFTNFCKLQNYTSVNIFSKDIF
                     EAIVN"
     gene            894..1013
                     /locus_tag="ACL73L_000783"
     CDS             894..1013
                     /locus_tag="ACL73L_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HGS0771790.1"
                     /translation="MPDIVFEIEFFHSDSLIFFYTSDDKSNSQKSQEDFSKNK"
     gene            complement(1109..2455)
                     /gene="glnA"
                     /locus_tag="ACL73L_000784"
     CDS             complement(1109..2455)
                     /gene="glnA"
                     /locus_tag="ACL73L_000784"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HGS0771791.1"
                     /translation="MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEG
                     EPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAY
                     HFLGGLIKHAYNYTVIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGT
                     RLELRSVDPMANPYIAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDL
                     PSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY"
     gene            complement(2492..2848)
                     /gene="glnR"
                     /locus_tag="ACL73L_000785"
     CDS             complement(2492..2848)
                     /gene="glnR"
                     /locus_tag="ACL73L_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HGS0771792.1"
                     /translation="MKEKEFRRNMAVFPIGSVMKLTDLSARQIRYYENQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRRALHNEL
                     LQQGRFASVQSPFGRG"
     gene            complement(2925..3452)
                     /locus_tag="ACL73L_000786"
     CDS             complement(2925..3452)
                     /locus_tag="ACL73L_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HGS0771793.1"
                     /translation="MSYFKKYKFDKSQFKLGMRTFKTGIAVFLVLLIFGFFGWKGLQI
                     GALTAVFSLRESFDESVHFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPIC
                     TMLTIMTNVAMNNKAGVIGGVAAMLIITLSIPSGETILYVFVRVLETFMGVFVAIIVN
                     YDIDRIRLFLEKKEK"
     gene            complement(3588..4784)
                     /gene="pgk"
                     /locus_tag="ACL73L_000787"
     CDS             complement(3588..4784)
                     /gene="pgk"
                     /locus_tag="ACL73L_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HGS0771794.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEA
                     AINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            complement(4874..9892)
                     /locus_tag="ACL73L_000788"
     CDS             complement(4874..9892)
                     /locus_tag="ACL73L_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HGS0771795.1"
                     /translation="MKNPFFERRCRYSIRKLSVGACSLMIGAVLFAGPALAEETAVPE
                     NSGANTELVSGESEHSTNEADKQNEGEHARENKLEKAEGVAIASETASPASNEAATTE
                     TASPASNEAATTETAEAASAAKPEEKASEVVAETPSAEAKPKSDKETEAKPEATNQGD
                     ESKPAEEANKTEKEVQPDVPKNTEKTLKPKEIKFNSWEELLKWEPGAREDDAINRGSV
                     VLASRRTGHLVNEKASKEAKVQALSNTNSKAKDHASVGGEEFKAYAFDYWQYLDSMVF
                     WEGLVPTPDVIDAGHRNGVPVYGTLFFNWSNSIADQERFAEALKQDADGSFPIARKLV
                     DMAKYYGYDGYFINQETTGDLVKPLGEKMRQFMLYSKEYAAKVNHPIKYSWYDAMTYN
                     YGRYHQDGLGEYNYQFMQPEGDKVPADNFFANFNWDKAKNDYTIATANWIGRNPYDVF
                     AGLELQQGGSYKTKVKWNDILDENGKLRLSLGLFAPDTITSLGKTGEDYHKNEDIFFT
                     GYQGDPTGQKPGDKDWYGIANLVADRTPAVGNTFTTSFNTGHGKKWFVDGKVSKDSEW
                     NYRSVSGVLPTWRWWQTSTGEKLRAEYDFTDAYNGGNSLKFSGDVAGKTVQDVRLYST
                     KLEVTEKTKLRVAHKGGKGSKVYMAFSTTSDYKFDDADAWKELTLSDNWTNEEFDLSS
                     LAGKTIYAVKLFFEHEGAVKDYQFNLGQLTISDNHQEPQSPTSFSVVKQSLKNAQEAE
                     AVVQFKGNKDADFYEVYEKDGDSWKLLTGSSSTTIYLPKVSRSASAQGTTQELKVVAV
                     GKNGVRSEAATTTFDWGMTVKDTSLPKPLAENIVPGATVIDSTFPKTEGGEGIEGMLN
                     GTITSLSDKWSSAQLSGSVDIRLTKPRTVVRWVMDHAGAGGESVNDGLMNTKDFDLYY
                     KDADGEWKLAKEVRGNKAHVTDITLDKPITAQDWRLNVVTSDNGTPWKAIRIYNWKMY
                     EKLDTESVNIPMAKAAARSLGNNKVQVGFADVPAGATITVYDNPNSQTPLATLKSEVG
                     GDLASAPLDLTNQSGLLYYRTQLPGKEISNVLAVSVPKDDRRIKSVSLETGPKKTSYA
                     EGEDLDLRGGVLRVQYEGGTEDELIRLTHAGVSVSGFDTHHKGEQNLTLQYLGQPVNA
                     NLSVTVTGQDEASPKTILGIEVSQEPKKDYLVGDSLDLSEGRFAVAYSNDTMEEHSFT
                     DKGVEISGYDAQKTGRQTLTLRYQGHEVNFDVLVSPKAALNDEYLKQKLAEVEAAKNK
                     VVYNFASPEVKEAFLKAIEAAEQVLKDHETSTQDQVNDRLNKLTEAHKALNGQEKFTE
                     EKTELDRLTGEVQELLAAKPNHPSGSALAPLLEKNKALVEKVDLSPEGLTTAKQSLKD
                     LVALLKEDKPAVFSDSKTGVEVHFSNKEKTVIKGLKVERVQASAEEKKYFAGEDAHVF
                     EIEGLDEKGQDVDLSYASIVKIPIEKDKKVKKVFFLPEGKEVVELTFEQTDSHVIFTA
                     PHFTHYAFVYESAEKPQPAKPAPQNKVLPKPTYQPASDQQKAPKLEVQEEKVAFHRQE
                     HENAEMLVGEQRVIIQGRDGLLRHVFEVYENGQRRLRSTEVIQEAIPEIVEIGTKVKT
                     VPAVVATQEKPAQNTAVKSEEASKQLPNTGTADANEALIAGLASLGLASLALTLRRKR
                     EDKD"
     gene            10185..11816
                     /locus_tag="ACL73L_000789"
     CDS             10185..11816
                     /locus_tag="ACL73L_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HGS0771796.1"
                     /translation="MSINWQEILFHFLGGLGLFLYSIKTMGDGLQQAAGDRLRFYIDK
                     YTSNPFFGVLVGIGMTALIQSSSGVTVITVGLVSAGLLTLRQAIGIVMGANIGTTVTS
                     FLIGFKLGNYALPMLFIGAVCLFFTKNRTVNNIGRILFGVGGIFFALNLMSGAMAPLK
                     DLQVFKDYMIELSKNPVLGVFVGTGLTLLIQASSATIGILQNLYAGNLIDLQGALPVL
                     FGDNIGTTITAIIASLGANIAAKRVAGAHVAFNVIGTVVCVIFLVPFTVLIHWFEATL
                     NLAPEMTIAFAHGTFNITNTIVQFPFIGALAYFVTKIIPGEDEVVKYEPLYLDEHFIK
                     QAPSIALGNAKKELLHLGNYAAKAFDLSYKYIIDLDEKVAEKGHKTEEAINTIDEQLT
                     RYLIALSSEALSQKESEVLTNILDSSRDLERIGDHTEALLNLTDYLQRKNVEFSDAAL
                     KELEEVYRQTSDFIKDALDSVENNDIEKARSLVERHEAINKIERVLRKTHIKRLNKGE
                     CSTQAGVNFIDIISHYTRVSDHAMNLAEKVFAEQI"
     gene            complement(12249..13856)
                     /locus_tag="ACL73L_000790"
     CDS             complement(12249..13856)
                     /locus_tag="ACL73L_000790"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HGS0771797.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGADNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPAADMT
                     EWSAMVDKVMNLKKQVKISLVGKYVELQDAYISVVEALKHSGYVNDVEVKINWVNAND
                     VTAENVAELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDIEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYTAFVTAAVENSN"
     gene            complement(14246..14833)
                     /gene="rpoE"
                     /locus_tag="ACL73L_000791"
     CDS             complement(14246..14833)
                     /gene="rpoE"
                     /locus_tag="ACL73L_000791"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HGS0771798.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
     gene            complement(14961..15410)
                     /locus_tag="ACL73L_000792"
     CDS             complement(14961..15410)
                     /locus_tag="ACL73L_000792"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS0771799.1"
                     /translation="MCFVPYYKVNHCEEAFAWYQDVNLVYLVDGVKLPYSQATLEAMY
                     SYLDRHGELFWIEVKEKGEWFPIGDITLSQDNLPIVIGNSAYQHRGLGKKILSALIEL
                     ARVKGWKELRVKEIYTYNYASRRCFKSLGFVENGATEKGRSFILELV"
     gene            complement(15415..15804)
                     /locus_tag="ACL73L_000793"
     CDS             complement(15415..15804)
                     /locus_tag="ACL73L_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HGS0771800.1"
                     /translation="MKVNIADLHPTQLYLSEKKLQDIQMLYQSAETIQVDPISILAFG
                     DCLLITDGHHRAYQALLAGRDTISAEWDRDGGDELYHLYAQACEERKIYSVFDLEDRI
                     LAQDGYEAKWYNWCDGFNQAATLLLKR"
     gene            complement(15832..16482)
                     /locus_tag="ACL73L_000794"
     CDS             complement(15832..16482)
                     /locus_tag="ACL73L_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HGS0771801.1"
                     /translation="MKDKQTFLMKGSFALLLFVILGYMVKFYPEMLVNFDQSIQTAIR
                     GDLPDYLTILFRSLTRLIDIPVIITWVVITAFVFYRKRWKIESFFMLGNLALAGLLIV
                     TFKNIYQRPRPAILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKTPVWRKIVQI
                     VLGLYLVSVLVSRVYLGVHYPSDVLASLCVGLGVLFIEFPFYDKLRFQWRFKGKQK"
     gene            complement(16472..17035)
                     /locus_tag="ACL73L_000795"
     CDS             complement(16472..17035)
                     /locus_tag="ACL73L_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HGS0771802.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGPVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TTLRFLLGSLAGTVGLTLAMLVLNYVYAVPLYAKFANFDIGKILGLSNYLMTMVLPFN
                     LIEGVIFSVSFWLLYVLLKPTLKHYER"
     regulatory      complement(17200..17434)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(17488..17991)
                     /locus_tag="ACL73L_000796"
     CDS             complement(17488..17991)
                     /locus_tag="ACL73L_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HGS0771803.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFISQMKGKLYLISKFAEKVYSEVDLSTDEDHYFLFG
                     REDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     gene            complement(18184..19014)
                     /locus_tag="ACL73L_000797"
     CDS             complement(18184..19014)
                     /locus_tag="ACL73L_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HGS0771804.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLAAMISYDTRMLL
                     LIAIFSVFLLYLSEIRFKDVSFVAVFATIFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGVYTLTSQELFYLLNLAIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQALKKGDYITLLLATLFLVVSLLLILQNQGRFYNPWK
                     "
     gene            complement(18999..20681)
                     /locus_tag="ACL73L_000798"
     CDS             complement(18999..20681)
                     /locus_tag="ACL73L_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0771805.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGIIPNIYKGQTYGEFLIKGQVAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLISLLNQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQDIIELIDQIHKEEGTTTLIIEHRLEDVLHCPVDRIV
                     LINDGRILFNGSTDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKEEQLADLDNLYIS
                     KGQVQLRTELVKETPELQSLFRLEDVSFSYDDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKALSSFIQTEGRYLWEGQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LGLRLRGVDEQEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHQQGHTIVMITHDMQLMLDYSDRALVMVDGELIA
                     DTVPASLLSDPELLVKANLKETSIFNLAKKLDVDPLDLTAFYKERREGCKLN"
     gene            complement(20783..21040)
                     /locus_tag="ACL73L_000799"
     CDS             complement(20783..21040)
                     /locus_tag="ACL73L_000799"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771806.1"
                     /translation="MKIKEQTRKLAAGCSKQCFEIVDRTDEVSSKHGFEVVDETDEVS
                     NHTYGKAKLTWFEEIFEEYKMMGKAGQLVFFDVYRLVRQVS"
     gene            complement(21047..21595)
                     /locus_tag="ACL73L_000800"
     CDS             complement(21047..21595)
                     /locus_tag="ACL73L_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HGS0771807.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINIPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGLIGHAIKDSLAGYGLWWTWIIASGLFGLVVGLFRKYVRVING
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLKKD"
     gene            complement(21610..22458)
                     /locus_tag="ACL73L_000801"
     CDS             complement(21610..22458)
                     /locus_tag="ACL73L_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HGS0771808.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGHITFEEVGPELSV
                     DQIVELPVVATIIEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVYQNQVVYGKSFADVRIGQPILYINSLYRLGLAINQGSFAKAYNVGVGSSWTIE
                     IKKIEG"
     gene            22646..23995
                     /gene="trkA"
                     /locus_tag="ACL73L_000802"
     CDS             22646..23995
                     /gene="trkA"
                     /locus_tag="ACL73L_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HGS0771809.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEQDEAVLNHIVSRFD
                     IIGILGNGADFAILEQASVQDCDIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIERDHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIDTKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKGVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            23999..25438
                     /locus_tag="ACL73L_000803"
     CDS             23999..25438
                     /locus_tag="ACL73L_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HGS0771810.1"
                     /translation="MNKSMIRYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSGQIPSLIDAF
                     FEISSGFTTTGASILNDISVLSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKLKNTAQILYLLYIALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLVFGVNFNLYYYLMLRRVKAFFGDEELRAYL
                     VIVLVSTGLISLNTLYLYPGFSKSFEMTFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMAIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNKMVIDKDTQHK
                     ILKYFVIYAMILLALIFIVSLDSNDFLVVTSAVFSCFNNIGPILGTTSSFSIFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
     gene            complement(25563..25964)
                     /locus_tag="ACL73L_000804"
                     /pseudo
     CDS             complement(25563..25964)
                     /locus_tag="ACL73L_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359733.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS alpha-glucoside transporter subunit IIB"
     gene            27372..27656
                     /locus_tag="ACL73L_000805"
     CDS             27372..27656
                     /locus_tag="ACL73L_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771811.1"
                     /translation="MDWYDYMIQASKQSQFNASHWFRYLRKVIFEDYSYLTNQDVEKL
                     LDSKELTRFQKISLKYAFQEHTPTHKYVISLNKPAKLTNVQKLMEKYKHG"
     gene            27649..27897
                     /locus_tag="ACL73L_000806"
     CDS             27649..27897
                     /locus_tag="ACL73L_000806"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771812.1"
                     /translation="MDKMKPVFQALNKELIQENLTLTIICVDGYVLEYHGLRATQDVD
                     AFYDQNQKINEIIARVGKQFNLNIHEELWLNNHVAKQI"
     gene            27993..28211
                     /locus_tag="ACL73L_000807"
     CDS             27993..28211
                     /locus_tag="ACL73L_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771813.1"
                     /translation="MMSIREQDLQDIGAIIKYKNFHSPFDTFKYLKDMGFDTIDLSVL
                     LEGFSYAYGMDWLEKFFKENQDKLREFY"
     gene            complement(28579..30903)
                     /gene="pflB"
                     /locus_tag="ACL73L_000808"
     CDS             complement(28579..30903)
                     /gene="pflB"
                     /locus_tag="ACL73L_000808"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HGS0771814.1"
                     /translation="MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIEPIRDEVLEFESVKANFEKSLDWLTDTYVDALNIIHYMTDRYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
     gene            31252..32313
                     /gene="dinB"
                     /locus_tag="ACL73L_000809"
     CDS             31252..32313
                     /gene="dinB"
                     /locus_tag="ACL73L_000809"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HGS0771815.1"
                     /translation="MLIFPLLNDLSRKIIHIDMDAFFAAVEIKDNPKLRGKPVIIGSD
                     PRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCPQAVFISGNYEKYKAVGLQIRAI
                     FKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAGISYNKF
                     LAKMASDYQKPHGLTVILPEQAENFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLE
                     VPEVTLIDRFGRLGYDLYRKARGIHNSPVKSNRIRKSIGKEKTYGKILRAEEDIKKEL
                     TLLSEKVALNLHQQEKAGKIVILKIRYEDFSTLTKRKSLAQKTQDASQISQIALQLYE
                     ELSEKERGVRLLGITLTGF"
     gene            complement(32460..33305)
                     /locus_tag="ACL73L_000810"
     CDS             complement(32460..33305)
                     /locus_tag="ACL73L_000810"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HGS0771816.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLIATLPLLGV
                     FKFDDWFDTHFHNMVSVALMLIIYGVAFIYLEKRNKARAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTSRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGQLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
BASE COUNT         9911 a         7172 c         6173 g        10181 t
ORIGIN      
        1 taaaattatc tgataataaa taatatttca aatatgtttc gttaagtaaa gtatccaaaa
       61 caataaatgc tgtactagct atcaaatact ctttaagttc tctaactaca gcaatatttt
      121 ttagatatgg tctaactgtt gaaaataaga cactattctg cgaaactaat tttctagcac
      181 gggaaggcgc ttgttcaggt gaaagatatt gtagattttt gtagttgatt atgttctttt
      241 ttctatcaat actagacgta tctatatacc taaaggattt ctctggctta ttttgcccaa
      301 aattccaata aattgatttt atcctcaccc actcccaagt atcaggaata tcataaggaa
      361 catcaatttc ttgagtactt ccatcagcaa acttcccata atgtttctta tgtgcttcaa
      421 gtatataaaa aggcgtaaaa atacgcctat agataatggg gttgaaatag gtttattgtt
      481 gatgagattg tagataattc aattttttac ttccaatcga atattcaaaa cctccacctt
      541 ttctgcctgt aattgttcat cataaaattc aatatcttca ggattttccc cttgacaacc
      601 tcggcagaaa tattcttccg ctcgatcagg attcaaaaat cgacaagcac aaacaaaaca
      661 gtcgccatca tcatttattg agataatata gtagattgaa ataagatgta aacaaatcga
      721 ttaggaaagt taaattaatt tctagaaatt tttagcagat gtagtgtact attctagtct
      781 caatttacta tggcttcaaa tatatctttc gaaaaaatat ttacagatgt gtaattttga
      841 agcttgcaaa agttagtaaa cttgtagatt tcgatttgaa gtaacttgtt ttcttgcccg
      901 atattgtttt tgaaattgaa tttttccata gtgactcctt gattttcttc tacacgtctg
      961 atgataaatc taattcgcaa aagagtcaag aggatttttc gaaaaataaa tagcgaccga
     1021 aatcgctatt ttaagggtta taggtatttg atggcttaga ctgctgtgtg actgtttacc
     1081 cacaggcaat ctttcttcta tattagtatt agtaaaggtc taaataatta tcaatttccc
     1141 attgtgaaac gaaggttgca taacttgccc attcgattcg tttggcttca aggaagctag
     1201 tgtagatgtg atctccgaga gcagctttaa ccacttcatc ttctgtcaaa gctttcaaag
     1261 cgttgtgaag agttgatgga aggtctgtaa taccagcttc cttgcgctct tctgctgtca
     1321 tgatgtagat attttcttcg ataggagctg gtgcttcgat tttattttca ataccataca
     1381 aaccaacttc caaaagaaca gccatagcaa tgtaagggtt cgccattgga tccactgaac
     1441 gcaactcaag acgagttccc ataccacgtg aagcaggtac gcgcacaagt ggcgaacggt
     1501 tacgaccagc ccaagcaatg taaacaggcg cttcataacc tggaaccaaa cgtttgtatg
     1561 agttaactgt tgggttcatg atgacagtat agttgtaagc atgcttgatc aaaccgccta
     1621 ggaaatggta agctgtttct gacaactgca ttccttttgg atcatttgga tcaaagaagg
     1681 cgttatttcc ttctgcatca aacaaggaca tattacagtg catacctgag ccagcaatac
     1741 caaattttgg cttcgccata aatgttgcgt aaagtccgtg tttgcgagca atggttttaa
     1801 caacaagttt aaagatttga atcttatcac aagcacggag aacttcatcg tacttaaagt
     1861 caatctcatg ctgtccaacc gcaacctcgt ggtgactcgc ttctacttca aatcccattt
     1921 tggtcaagac attcacaatc tcacgacgtg tgttgtccgc aaggtcagta ggtgccaaat
     1981 caaagtagcc acccttgtca ttcacttcaa gtgttgggtc cccattttca tccaacttaa
     2041 ataggaagaa ttctggctct ggaccaaggt tgaaggattt gaatccaact tcttccatgt
     2101 gacgaagagc tcgtttcaaa ttaccacgag ggtcacccgc aaatggttca ccttctgttg
     2161 tataaacatc acagatcaga cctgcaacac ttccattttc atctccccaa gggaagactg
     2221 tccatgtatc caagtccggg tacaagtaca tatccgactc attgatacgt acaaaacctt
     2281 caatagaaga tccatcaaac ataaccttgt tcgacaagac cttatctaac tgttcatctg
     2341 tagcaggaat ttcgacgttt ttcatggttc ccaaaatatc tgagaacata agacgaataa
     2401 aggtaacatt tttttccttg acttcacgac gaatatctgc agctgtgatt ggcataagtt
     2461 ttctccttaa tctatgacta cttgcggttg cctaaccgcg accaaaaggt gactgtactg
     2521 aagcaaaacg cccctgttgg aggagttcat tgtgaagtgc acgacgtact tcagtctgac
     2581 taaccgcttt cttggatttc gcttcacgtt cagcatattt tttcttaatg gcagcgatat
     2641 tataaccttc agagatataa tctttgattt caagcagacg atccatgtca ttcaaggaat
     2701 acatgcgacg atttccttcg tttcgatcgg gcttgatcaa ctcttgattt tcataataac
     2761 gaatctgacg cgccgataga tcggtcaact tcataacact gccgatagga aaaacagcca
     2821 tatttcggcg aaattctttt tccttcattt acaatttcct tctttctgtc tattatagtc
     2881 taaaaaaaga caaacgtcaa ttaataatgt tataaaatgt aacattattt ttcttttttc
     2941 tctaaaaaga gacgaatacg atcaatatcg taatttacga taattgcgac aaaaactccc
     3001 ataaatgttt ctaatacacg cacaaacacg tacaaaattg tctcaccact tggaattgat
     3061 agggtaataa ttaacatagc tgctacacca ccaataaccc ctgctttgtt attcatggct
     3121 acatttgtca taatggttaa catggtgcag attggaacaa ctaccaaggt cacccaaaag
     3181 gcttcgtgga aaaaggtatt taataagaag aagaccaagg catagagtcc accgatacta
     3241 tttcctagaa tacgcgaagt cccaaaatga acactctcat caaaactctc cctcaggcta
     3301 aaaacggctg tcaaagcacc aatttgaaga cctttccagc caaaaaagcc aaaaatcaag
     3361 agaactagaa aaacagcaat acctgtttta aaggttcgca taccaagttt gaactgggat
     3421 ttatcgaatt tatatttttt aaaataactc ataatctcaa ctttctattt ccattttatc
     3481 ataaatcggt gatttttatg agtaatagtt gagaggaagc gtttttattt taagcaaaag
     3541 aaaagaggaa ctttcatccc tctcttcttt gatttattta taaaatctta tttttctgtc
     3601 aaggctgcaa gtcctggaag aaccttacct tcaagaagtt ccattgatgc tccaccaccc
     3661 gtactaatcc atgagaactt gtctgcacgg ccaaggttaa tcgctgcggc agctgagtca
     3721 ccaccaccga tgattgattt aactcctggt tgtttcacga tagcgtccat cacaccgatt
     3781 gtaccagctt ggaaatctgg gttttcaaat acacccatag gtccgttcca tacaactgtt
     3841 ttggcaccag tcaaagcttc gtcaaatttg gcgatagatt ttggaccgat gtcaagacca
     3901 aggaagcctt cagaaactgc ttcaccttca gtgtcacgca cttcagtgta accagcaaat
     3961 gcgttagctt cttttgagtc aactggcaag atcaatttac catttgcttt ttcaagaaga
     4021 gctttcgcaa catccaattt gtcttcttct acaagtgagt taccgatttc gataccttgt
     4081 gctttgtaga atgtgtaagt catcccacca ccgataagga ctttatcagc tttttcaagc
     4141 aagttttcga taacaccgat cttgtctgaa acttttgaac caccaaggat agccacgaat
     4201 ggacgttctg gagtttcaac tgcttcttgg atgtaggcaa tttcgttttc aagaaggaaa
     4261 ccagcaactg ctttttcaac gtttgctgag ataccaacgt tagatgcgtg tgcacggtga
     4321 gctgtaccga atgcatcgtt tacgaagata ccatctccaa gtgatgccca gtatttacca
     4381 agttcaggat cgtttttaga ttctttcttg ccgtcaacat cttcgtaacg agtgttttca
     4441 accaagagaa cttgtccatc ttcaagagcg ttgattgccg cttccaattc agcaccacga
     4501 gtgacacctg ggaaaacaac atcttgacca agttttgctg ccaagtcagc tgctacagga
     4561 gcaagtgatt taccagcttt atcagcttct tctttcacac gtccaaggtg agagaaaaga
     4621 attgcacgtc caccttgttc gatgatgtac ttaatagttg gaagagctgc tgtgatacgg
     4681 ttatcgttag tgattacgcc atctttcaat ggtacgttga agtcaacacg aacgaggact
     4741 tttttacctt tcaagtcaac gtctttaaca gtaagttttg ccatgttaca aaaactcctt
     4801 tatttatttt attagaaact attatatcac actttggaaa tataaggaaa gatttcacaa
     4861 gatttttcga tatttaatct ttatcttctc ttttccgtct caaggtcaag gctaaactag
     4921 caagaccaag gctggctaag cctgctatta gggcttcatt agcatcagct gttcctgtat
     4981 ttggcaattg tttgcttgct tcttctgatt taactgctgt attttgagct ggtttttcct
     5041 gtgtagctac tactgctggt actgttttta cttttgttcc aatttcaaca atttctggaa
     5101 tcgcttcttg gatgacttct gttgaacgaa gacgacgctg accgttttca taaacttcaa
     5161 agacatgtct taacagtcca tctcgtccct gtatgatgac tcgttgttcc ccaactagca
     5221 tctcagcatt ttcatgctct tgacgatgaa aggcaacctt ttcctcttga acttccaatt
     5281 taggagcctt ttgttgatca gaagccggtt gataagtagg ttttggaagg actttgtttt
     5341 gtggtgctgg tttagcaggt tgtggttttt cagcagattc ataaacaaag gcataatgag
     5401 taaagtgagg tgctgtaaag ataacatgac tatccgtttg ttcaaaagtc aattctacta
     5461 cctctttgcc ttcaggtaag aaaaatactt tcttaacttt cttatctttt tcaattggga
     5521 ttttcacaat agaagcataa gagagatcaa catcttgacc tttttcatcc aaaccttcta
     5581 tttcaaagac atgagcatct tctccagcaa agtatttctt ctcttcagca cttgcttgaa
     5641 cacgctctac tttcaaaccc ttgatgacag tcttctcttt atttgagaag tgtacttcaa
     5701 cacctgtttt actatcagaa aagactgctg gcttgtcttc tttcaataaa gcaaccagat
     5761 cttttagact ctgtttcgct gttgtaagcc cttctggact caaatctact ttttcaacca
     5821 aggccttgtt tttctcaaga agcggagcta gagcagaacc tgaaggatgg tttggtttgg
     5881 cagccaagag ttcttgaacc tcacctgtta aacgatcaag ctctgtcttt tcttccgtaa
     5941 atttctcttg accattcaga gctttatgag cttctgtcaa tttattaagt cggtcattga
     6001 cttgatcttg ggtgctagtt tcatggtctt tcaacacttg ttcggccgct tcaattgctt
     6061 tcaagaaagc ttcttttact tctggtgaag caaagttata gaccaccttg ttcttagcag
     6121 cttcaacttc tgctaatttc tgtttgaggt actcatcgtt caatgctgct tttggagata
     6181 ccaaaacatc aaagttgact tcatgacctt ggtaacgaag cgtcaaggtt tgacgaccag
     6241 tcttttgagc atcataacca gaaatttcaa ctcccttatc agtaaaggaa tgttcttcca
     6301 tggtgtcatt gctataagcc actgcaaagc gtccttcaga caagtctaag ctatcaccaa
     6361 ctaggtaatc ttttttcggt tcctgactta cttcaattcc caaaatagtt ttcggacttg
     6421 cttcgtcttg gccagtgaca gtcactgaca aattagcatt taccggttgt cccaaatatt
     6481 ggagagtaag attctgttct cccttatgat gcgtatcaaa acctgatact gatacacctg
     6541 cgtgagttag gcgaatgagt tcgtcctcag ttcctccttc atactgaact cgaagaacac
     6601 cacctctaag gtccaaatcc tccccttcgg cgtagcttgt tttcttaggt cctgtttcta
     6661 ggctgactga cttgattctt ctgtcatctt ttggaacgga aactgctagg acattactaa
     6721 tttccttgcc tggcaactgg gtacgataat aaagaagacc agattgattt gtcaaatcca
     6781 atggtgcact tgctaggtct cctccaactt cgctcttcaa ggttgcgagt ggagtttgag
     6841 aatttggatt atcataaacg gtaatagttg ctccagccgg tacatctgca aagccaactt
     6901 gtaccttgtt attgcctaga gaacgggctg cagccttggc catcggaata ttgacactct
     6961 cagtatcaag cttttcatac attttccagt tatagatacg aatagccttc catggagttc
     7021 cattgtcaga agtgacaaca ttcaagcgcc agtcttgagc agtgattggt ttatcaagag
     7081 tgatatctgt cacgtgtgct ttgttgccac ggacttcctt ggctagcttc cactcaccat
     7141 ctgcatcttt ataataaagg tcaaagtctt tggtgttcat caagccatcg ttaacagact
     7201 caccaccagc ccctgcatga tccatgaccc atctaacaac ggtacgtggc ttggtcaaac
     7261 gaatatccac actaccactc aactgagctg aagaccattt atctgacaag ctagtaatgg
     7321 taccgttcaa cataccttca ataccttctc caccttcagt cttagggaaa gtactatcaa
     7381 taactgttgc acctggaacg atattttcag ctagtggttt tggtaggctg gtatctttta
     7441 cagtcatacc ccaatcaaag gttgtggttg cagcttctga acgaactcca tttttaccga
     7501 ctgctacaac cttcagttct tgagttgtac cctgagcact tgctgagcgg ctaacttttg
     7561 gtagataaat agttgtagaa gatgagccag ttagtaattt ccagctgtct ccatcttttt
     7621 cataaacttc atagaaatct gcatccttgt tgcctttaaa ttgcacaact gcttccgctt
     7681 cttgggcatt tttaagagat tgtttcacta cagaaaagct tgtcggcgat tgtggctctt
     7741 ggtgattgtc cgagatagtt aattgtccga ggttgaactg ataatctttt acagcacctt
     7801 catgctcgaa aaatagtttg actgcataga tggttttacc cgctagtgag ctgagatcaa
     7861 attcttcatt tgtccagttg tcagaaaggg ttagctcttt ccatgcatct gcatcatcga
     7921 atttgtagtc tgaagttgta gagaatgcca tataaacttt agaacctttt cctcccttgt
     7981 gggcaacacg aagtttggtc ttctcagtta cttctaactt agtagaataa agtctcacat
     8041 cctgaactgt cttaccggct acatcaccag agaatttaag ggaatttccg ccattatagg
     8101 catctgtaaa atcatattct gcacgaagtt tttcccctgt tgaagtctgc caccagcgcc
     8161 atgttggaag aacacctgat actgaacgat aattccactc agaatcctta gaaaccttac
     8221 catctacgaa ccatttttta ccatgacctg tattaaaaga agtagtaaaa gtattaccta
     8281 ccgctggcgt acggtccgca actaggttag caataccata ccaatctttg tcacctggtt
     8341 tttggccagt ggggtctcct tgataacctg taaagaagat atcttcattt ttatgataat
     8401 cttcaccagt ttttcctaaa cttgtaatgg tatctggggc aaataaacca agagaaaggc
     8461 gcaatttccc attttcgtct aaaatgtcat tccacttaac ctttgtcttg taggaaccac
     8521 cctgttgcaa ttccaaacct gcaaatacat cataaggatt acgaccaatc cagttggcag
     8581 ttgcaatagt gtaatcattt ttagccttat cccagttaaa gttagcaaag aagttatctg
     8641 ccggaacctt atctccttct ggttgcatga attggtagtt gtattctccc aaaccatctt
     8701 gatggtaacg tccatagtta taggtcatgg catcatacca agaatacttg attggatggt
     8761 ttaccttagc agcatattcc ttgctataga gcataaactg gcgcatcttt tctccaagag
     8821 gtttaaccaa atctccagtt gtttcttggt tgatgaaata gccatcatag ccataatact
     8881 ttgccatgtc taccaattta cgagcaattg ggaagctacc atctgcgtct tgcttcaaag
     8941 cttcagcaaa tctttcttga tctgcaatac tattagacca gttgaagaag agtgtaccgt
     9001 atacaggaac cccgttacgg tgacctgcat caataacgtc aggagttggt acgagacctt
     9061 cccagaagac cattgaatct agatattgcc aatagtcaaa agcataggcc ttgaactctt
     9121 ctccaccaac agaagcatgg tcttttgctt tagaattagt gtttgataag gcttgaactt
     9181 ttgcttcctt gctagctttt tcattgacta aatgacctgt ccgacgtgaa gcgaggacaa
     9241 cagatccgcg gttaatagca tcatcttcac gagcacctgg ttcccatttt aacaattctt
     9301 cccaagaatt aaatttgatt tcctttggtt ttaatgtttt ttctgtattt ttagggacat
     9361 ctggctggac ttctttttca gtcttattag cttcttctgc tggtttagac tcatcccctt
     9421 ggttagttgc ttcgggcttt gcttctgttt ccttgtcaga cttaggtttt gcttctgcag
     9481 atggtgtttc tgcaaccacc tcacttgctt tttcctctgg tttagctgcg ctagctgctt
     9541 ctgcagtttc agtagttgca gcttcattgc ttgctggcga agcagtttca gtagttgcag
     9601 cttcattgct tgctggcgaa gcagtttcag atgctatcgc tactccttct gccttttcta
     9661 gcttgttttc tctagcatgt tccccttcat tctgcttatc agcttcattg gtcgaatgct
     9721 cactctctcc tgaaacaagc tctgtattag ctccgctatt ttcaggaact gcagtttctt
     9781 cagccaaggc tggaccagca aataaaacag caccaatcat cagcgagcag gctcctactg
     9841 ataacttacg aatactgtaa cgacaacgtc tttcaaaaaa tggattcttc attttctcct
     9901 cctaaaagat tgattttgtt gttttcaaga aagcgctttg tattctgtgg ttttttatca
     9961 aactctagaa ccatgataat cacattttta tcctaaaaca tttttagaca aaaaacaata
    10021 gctgttttca caaatctaaa ataaggttaa aaactcttaa aaataataca cttatcaatt
    10081 ataattttct tttacaaact tgacattaac acttgttaga tttactttta tcctttaaaa
    10141 tagaatagga gatattccgc gattattttt cggaggaaaa agaaatgtcc attaattggc
    10201 aggaaatttt atttcacttt ttaggtggcc tggggctatt cttatatagt atcaagacca
    10261 tgggagacgg tttgcaacaa gctgctggag atcgccttcg tttttacatt gacaaatata
    10321 ctagtaatcc tttctttgga gttctggttg gtattgggat gactgctcta attcagtcta
    10381 gttctggtgt aacagttatt acagtcggcc tggtcagtgc cggtctctta accttacgtc
    10441 aggctatcgg gattgtcatg ggtgctaata ttgggacaac tgtcacatcc tttctcatcg
    10501 gttttaaatt aggtaactat gccctaccta tgctctttat cggtgccgtc tgtctctttt
    10561 ttacgaaaaa tcggacagtc aataatatcg gacgcatcct ctttggtgtc ggtggtatct
    10621 tttttgccct caatctcatg agcggcgcaa tggctccact caaggattta caggtcttta
    10681 aggactatat gattgagcta agtaagaatc ctgttttggg tgtctttgtc ggtactggct
    10741 tgaccttgct aattcaagct tcttcggcta ccattgggat tttacaaaac ctctacgccg
    10801 gcaatctaat tgacctacag ggagctttgc cagttctatt tggtgacaat atcgggacaa
    10861 ccattacagc catcattgcc tctttagggg ctaatattgc agctaaacgg gtagcaggag
    10921 ctcatgttgc cttcaacgtt atcggaacag tcgtctgcgt tatttttcta gttcctttta
    10981 ctgtcctgat tcattggttt gaagctacgc taaatctagc accggaaatg accatcgcct
    11041 ttgctcacgg aacctttaat attaccaaca ccattgtcca atttccattt atcggagctc
    11101 tggcttactt tgtaaccaag attattcctg gagaggacga ggttgtcaaa tacgaaccct
    11161 tatatcttga tgaacatttc atcaaacagg ccccatctat cgctctagga aatgctaaga
    11221 aagagctctt gcacttagga aactacgctg ctaaagcctt tgacctttcc tataagtaca
    11281 tcattgactt ggatgaaaaa gttgctgaaa aagggcataa aaccgaagaa gcaattaaca
    11341 ccatcgatga gcaattaaca cgttatctca ttgccctttc aagcgaagct ctcagccaaa
    11401 aagaaagtga agtgcttacc aatatccttg attcctcccg tgatttggaa cggattggag
    11461 accacacgga ggctctactc aatctgactg actatcttca acggaaaaat gttgaatttt
    11521 ctgatgccgc cttgaaagaa ttagaggaag tttaccgcca aactagtgac tttatcaaag
    11581 atgctctgga tagtgtggaa aacaatgata ttgaaaaagc acgcagtctt gtagaacgtc
    11641 atgaagcaat caataagata gaacgtgttc tcagaaaaac ccacatcaaa cgtctcaaca
    11701 aaggcgaatg ttcaacacaa gctggggtca actttatcga catcatctca cactacactc
    11761 gtgtatcaga ccacgctatg aaccttgctg aaaaggtttt tgcagaacaa atctaagaac
    11821 caagaagcta tccatcataa ttggatggct ttttactttt tcctaagcaa gactaggctg
    11881 aatgaaactg aaagagtatt ctgtagatat atagtatatt gaaactagaa tagtacacct
    11941 ctatttctaa aacattctta gaaatcgatt tgactgtcct gatagagatg ttcacatctt
    12001 atttcaattc actatacaat ccagttccta aaactttgct agaaattgat ttggctttcc
    12061 gaattaattt gttcaaattt tatttcattt caatatactg ataaatccat tcactagcac
    12121 agggcaagag ggagtcgcta tttatttcaa cggtagcagg tacatacaga ggactcctat
    12181 ctcagatata ctgcaatatc atcgtatgca aaaaacctct gagatttttc tcaaaggttc
    12241 tgattttgct aattgctgtt ctcaaccgct gcagtaacaa aggcagtgta gagttcttct
    12301 ggtcggtttg gacggcttga cagttcaggg tgatactgac aagctacaaa gaatttattt
    12361 tcaggaattt ccacgatttc taccaaacga ttgtctggag aaactcctga aaagacaaaa
    12421 cctgctgcct caaactgctc acggaaggca ttattaaact cataacggtg acggtgacgg
    12481 cgttgcacca cttcttgatt gtgataagca gcagcagcct tagagccacg tttcaactta
    12541 gacggataaa gtcccaaacg aagggttcca cccatatcct caatatcaat ctgatcacgc
    12601 atgatatcaa tgatagggta ttttgtttct ggtgcaagct ctgcagaatt ggcaccttca
    12661 agacctaaaa cgtgacgagc aaactcgata catgtcaact gcattcccaa gcagactccc
    12721 aacattggaa catcattttc acgcgcatag cggatggctt ggattttccc ttctgtacca
    12781 cgttgaccaa aaccacctgg tacgatgatc ccgtccgcat cagacaagag ttctgctaca
    12841 ttctctgctg tcacatcatt ggcattgacc caattgattt taacttctac atcattgaca
    12901 tagccagagt gtttcaaggc ttcgaccact gagatatagg catcttgcaa ctccacatac
    12961 ttaccaacaa gggaaatctt aacttgtttc ttgaggttca tgaccttgtc caccatggct
    13021 gaccattctg tcatatccgc tgctggtgcg tctaatttca aatgatcaca aacaatttgg
    13081 tccatccctt gtgcctgcaa gttcagtgga atttggtaaa ggtgttcaac atccaacgat
    13141 tcgataacgg cttctggtgc cacatcacag aactgggcca gtttattttt aattccttga
    13201 ccagctggct cttctgtacg aataaccaac atatttggtt ggattcccaa gccacgcaat
    13261 tctttgacag agtgttgggt tggtttggtt ttcatttcac cagcagcctt gaggtaagga
    13321 agcaaggttg tatggatata catgacatta tccgcaccca catctgcctt catctgacga
    13381 agagcctcta ggaatggcaa ggactcgata tctcctactg ttccaccaac ctctgtgata
    13441 atgacatcag agtcggtcgt tagagcggca cgcttgattt tttctttcaa agcatctgtg
    13501 atatgaggaa tgacttgaac agttgcccca aggtattctc cacggcgttc tttacgaaga
    13561 acttcactgt aaattttccc agttgtcacg ttggaatatt tgttgagatt gatatcgatg
    13621 aaacgttcat agtgacccaa gtccaaatct gtctcagctc cgtcatctgt cacaaaaact
    13681 tccccgtgct ggtaaggact catggttccc ggatcaatat tgatataagg gtcaaacttt
    13741 tgaatggtta ctttgagacc acgatttttc aagagacggc ctagactcgc tgccacaatc
    13801 cctttcccaa tggacgatac cacaccacca gttacaaaaa tatatttcgt agacatagat
    13861 tcctctttct aaaatgctca aggtcttgtt aactacgagg ggatatagaa aacaagaccc
    13921 tactaataag aataatctgg gacgactgca ccagattcac aactaatact cttcgaaaat
    13981 ctcttcaaac cgcgtcaacg tcgccttgcc gtagatatgt gttcctgact ttgtcagtct
    14041 tatctacaac ctcaaaacag tgttttgagc agcctacggc tagtttccta gcttgctctt
    14101 tgattttcat tgagtataaa atacaagaag ataacttctt gaaaaaacaa aaatagctcc
    14161 ctaagaacta gggagccccg acctctaaaa gaggtgccca aacaatatga tacctaaaaa
    14221 taggataatt gtcaatagct aactcttatt cctcgctggt ttcagcgtcg tcatctgaaa
    14281 actcgtcgtc gtcttcgttg agatccacat cttctcccaa gtcatctgga gcgatttcgt
    14341 tgatttctgc atcataagct tccacttcat ttttttcatc atctggattt tcatcatcgt
    14401 atgaaagagc tggatctgct tcgtatgcat cttcgtcttc tggatcatct gcattgtagt
    14461 caatggcatc tgaatcacca tccataaagg cattgacacg ttttttctta gcttttggtg
    14521 ctacttcatc gtcgtcattt tcttcaagag cgatgatttc ttcgtcgatt tcgtccacac
    14581 cataccatga acgaagaccc catttgttgt ccccaagtga gatgaagcta ccgtcaaagt
    14641 tcaactctgt gtagaacaaa ggcaaagctt cgcggatatc gctgtttgat gttccaaggt
    14701 agttttgaat ttcgtttaca agatcgctaa aatgcatctc atgatcgcga ccacgaagtt
    14761 ccaagatagc acgcgctacc tcaatcatag atagttcact tttttcttgc ccagcaaata
    14821 cttctaattc caaagcgttt ctcctcattt atactactat cgccagagcg aacagactct
    14881 gacctcattt tatcatttac tctttatttt acgataattt tgcggaatag tcaaaggtta
    14941 agggggagaa agtggcagga ttagactaat tccaatataa aactccttcc tttttctgtt
    15001 gctccatttt ccacaaatcc aagcgacttg aaacacctcc tagaagcata attgtaggtg
    15061 tagatttcct tgactctcaa ttctttccat ccttttactc gagccaattc aatcaaagca
    15121 cttagaatct tttttccaag tcctcgatgt tggtaagcgg aattcccaat cacaatgggg
    15181 agattatcct gagatagtgt aatatcccca attggaaacc attctccctt ctccttgact
    15241 tcaatccaaa aaagctcacc atgccgatcc aaataggaat acatggcttc caaggtcgct
    15301 tgactgtaag gaagcttcac cccatctacg aggtaaacca agttcacatc ctgataccaa
    15361 gcaaaagctt cctcacaatg attgacctta taataaggaa caaaacacaa tgcactacct
    15421 tttcaacaaa agagttgctg cttgattaaa accatcacac cagttatacc attttgcttc
    15481 atacccatct tgagctagga tacgatcttc taaatcaaaa acagagtaaa tctttctttc
    15541 ctcgcaagct tgcgcataga gatgatatag ttcatcacca ccatctctat cccactcagc
    15601 agaaatcgta tcccgacctg ccaataaagc ctgataagcc ctgtgatgcc catctgtaat
    15661 cagcaaacaa tctccaaagg caagaatact gattggatcg acttggattg tttctgccga
    15721 ctggtaaagc atctgaatat cttgcaactt cttttctgat aaatatagtt gagtcggatg
    15781 aagatctgct atattgactt tcatttcttt ctcctcaagg gaattcgata ctcacttctg
    15841 tttgccttta aatcgccatt ggaagcggag cttgtcatag aagggaaact cgataaacag
    15901 gactcccaag cccacacaga gactggcaag gacgtctgat gggtaatgaa ctcccagata
    15961 gactcttgat accagcacac tgactaggta gaggccaagg acgatttgta cgatttttct
    16021 ccagactgga gttttaattc gttgactgag aatgacaatc agagtaccta ccatcaaagt
    16081 tacagccaga gaatgcccac ttgggaagga aaatcccttc tcttccacca gatgtaagat
    16141 agctggtcgt gggcgctggt agatattttt aaaggtcacg attaaaagac ctgccaaagc
    16201 cagatttccc agcatgaaga aactttctat cttccatcgc ttacgataaa agacaaaagc
    16261 tgtaatgaca acccaagtga taatcactgg gatatcaatc agacgtgtga gggatcgaaa
    16321 aagaatagtc aaataatctg gtaagtctcc tcgaatggca gtctgaatcg attggtcaaa
    16381 attgaccaac atttcagggt aaaatttgac catatagcca agaataacga aaagtaaaag
    16441 ggcaaaactg cccttcatta aaaatgtttg tttatctttc atagtgtttt aaggttggtt
    16501 tcaaaaggac gtacaacaac cagaatgaaa cggaaaagat tacaccctca atcaagttaa
    16561 aaggtaatac catggtcatt aggtagttgg aaagtcccaa aatttttcca atatcaaagt
    16621 tagcaaactt agcgtacaaa ggaacagcat aaacatagtt gagaaccaac atggccaagg
    16681 ttaaaccaac agttccagct agagagccta gtaggaaacg aagggttgtc cgttcctttt
    16741 tccaaatcaa agcaaatacg atgacaaaaa ctcccaaagc tacgatattc atcggcaaac
    16801 caatgtaagt attcactcct tggctgttaa gaagcaattt caagagtgag cgaagcaaga
    16861 gcactcctag agcagcaggc aaatccatga ccaccggacc cacaaggact ggcaagatac
    16921 taaattcgat cttgaggaaa gatgccgctg gtaaaagcgg aaagtcaaag tacatcagca
    16981 caaatgagat ggctgataaa attgcaatgg tcgaaagtcg acgtgtgttt gtcataacag
    17041 gttcctccaa ttttctataa aatcagaaga agttggaaag gattcctcta tctattctca
    17101 ctttttatat cccaaaagtt ccctcttact ctattaaaga aaaacaaagc aagtggttac
    17161 aatccggcta taaatctatc aaaacagaca aagctattct ttcgtcttct cccatccaga
    17221 ctatactgtc ggttgtggaa tctcaccaca tcagcttgcg ctcgcggact tctttaaaga
    17281 gaaggaaata gagatttttc ttttagacta ggcgacaagc tgtaggcata acttctggtt
    17341 aggcaaagct tgtcaacaaa gtataaaagg aaaagatcat ttcctagtta aagtcaccgc
    17401 cggtcgggaa tcacaccctg ccctgaagac tcttttatca taacaaaaaa cgcttgcaag
    17461 cgcaagcatt ttgttcattt tcatttttta tttcaattta tccacttcat aggtatggac
    17521 gagctcaaga cctgcaaagt tctgctggcg aagagcctca tagacaatca tgcagacggt
    17581 attagacaca ttgagactgc ggacatgttc atcattcatg ggaatacgga gagccttctc
    17641 aggatgttct cgcataaaat cttcaggtaa gcccttgtct tcacgtccaa agagaaaata
    17701 atggtcctcg tcagtcgata aatccacctc agaatagact ttttccgcga atttcgaaat
    17761 cagatagagt tttcccttca tctgagatat gaaatcttcc aaactctcgt aaaaataaat
    17821 ctctagctta tcccagtaat ccaatccagc ccgcttcatc ttgcggtcat caataggaaa
    17881 gcccataggc ttgatgatgt ggaggggaga attggtcgca gcgcaagtac gcgcgatatt
    17941 gcccgtattt tgtggaattt gaggttcaaa taatacaatg tgatttgtca tgacttgctt
    18001 cctttcacca ttgcaaaaaa atagccacac tgcccggagt caagctcagc aaacagcgtg
    18061 gttaaggcat cgttaactta cctcacaaca ggtttgaagt aaatcagcga aactactttc
    18121 ttagtataac actttcagaa tcattgtcaa tagaaatgac ttgatttttt caattttttc
    18181 aagctatttc caagggttgt aaaatcgtcc ctgattctgc aagataagta gtaaactaac
    18241 tactaaaaac aaggttgcca agagcaaggt aatatagtct ccttttttca aggcctgata
    18301 actataccat gtgcgttttt tctctttccc aaagcggcga agctccatgg cagtcgcaat
    18361 ggtatcaatg cgttctagcg agctaaaaat caagggcgta ataatgagca gattgccttt
    18421 gattcgttgc ataagagaag ctttcttgga taattccatc ccacgcgcct cctgagacat
    18481 cttgatagta aagaattctt cctgcaaatc tggaatatag cgcaaggtca ggctgacaga
    18541 ataagcaatc ttatagggca caccaatttg atttaaactg gaagcaaact gactaggatg
    18601 ggttgtcatc aaaaagataa tagccagagg aatggtgcaa agatacttaa tggccaaatt
    18661 tagcagataa aagagctcct ggctggttag agtgtagaca ccgattccct gccaaatcac
    18721 acttctctct ccataaagtc caaccccata ctcgggagaa aagagataga ccatcaaaac
    18781 gtttaaaacg gcaaatatcg tcgcaaaaac ggctacaaag gaaacatctt taaagcgaat
    18841 ttctgataaa tagaggagaa agactgaaaa gatggcaatc agcaagagca ttctggtatc
    18901 atagctaatc atggccgcca atgataccag aatgaaaaag agaagtttcc cagctcctga
    18961 caagcgatga atcacagtat ctctatgctg gtaaccgatt aatttagctt gcatccctct
    19021 ctcctttctt tgtaaaatgc cgttaaatcc agtggatcca catctagttt cttagccaag
    19081 ttaaagatgg aggtttcttt tagattggct tttactaaca gctcaggatc gctcaacaga
    19141 ctggctggaa cagtatcggc aatcaattct ccatccacca tgacaagggc tcgatctgaa
    19201 taatccagca tcaattgcat atcatgggta atcatgacaa tggtatgccc ttgttgatgc
    19261 agttcttcga gaaattccat aatctcagta tagttcttct ggtcttgacc cgcagtcggt
    19321 tcatctagga ggataatttc agcgcctaag accaaaattg aggcaatagt cacacgtttt
    19381 ttctgaccaa atgacagggc agaaatgggc caattacgga attcatagag accacagatt
    19441 ttcaaggttt catagactct cgtttcaatt tcctgctcgt ccacacctcg caaacggagt
    19501 cccagagcca cctcatcaaa aatcatattg gttgaaatca tttgattggg attttgcagc
    19561 acatagccta ctcgttccgc ccgctctgca acagaatctc cttttatatc ctgtccttcc
    19621 caaagatagc ggccttcagt ctggataaag ctactcaagg ccttggctag agttgacttc
    19681 cctgctccat tttttccgac aatggcaatc ttttcaccct ttttaatatc taaatgaaga
    19741 gattttaaaa tcggtctatc atcataagaa aacgacacgt cctctagtct aaagagtgac
    19801 tgcaattctg gggtttcttt taccagttcc gtccgcaact gaacctgacc ttttgagata
    19861 tacaagttat ccagatctgc taattgttct tccttgacta aatccacacc caattgacgg
    19921 agagtggtga gataaagggg ttcgcgaatt ccattttggg tcaacaaatc agtcgccagt
    19981 aactggtcag tgctcccatt aaaaaggata cgaccatcgt ttatcaagac aatccgatcc
    20041 acagggcaat gcagaacatc ctccaaacgg tgctcgataa taagggtcgt cgtcccctcc
    20101 tccttatgaa tctggtcaat caattcgata atatcctgac ctgacttggg atcaagattg
    20161 gcgagtggct catcaaacaa gagaatcgga ctctcatcaa tcaagacacc agccagactg
    20221 actcgctgct tttgcccacc agacaaatcc tgaggacgct gattcagtaa agaaataagg
    20281 tccagctttt cagcccattt ataaacacga cctttcatct catctagggc tgtcacatca
    20341 ttttccagag caaacgccaa atcttctgcc acagacaagc caataaactg cccatctgta
    20401 tcctgcaaaa ctgtgctaac cagatgagac ttatcataga tgctcatatc aaaggctact
    20461 tgacccttta tcaaaaattc tccatatgtc tgacccttgt aaatattggg aataatccca
    20521 ttcaaacact gacccaaggt agatttacct gacccagatg gtccaacaat taagactttc
    20581 tctcccttgt aaatggtcaa gtctatccct tgcaaggtcg gttcttgttg tgtttcatac
    20641 cggaaagaga aatccttcca ctcaattata gcttctttca tcttactctc ttcattcgct
    20701 tcttagactt ctattttatc ataaatcaag cccttcttgc agtctctcct cttaaaatct
    20761 tagcgccaaa aagattccta tcctagctta cttgcctaac taatctataa acatcgaaaa
    20821 agactagttg cccagccttc cccatcattt tatactcttc gaaaatctct tcaaaccacg
    20881 tcagcttcgc cttgccgtag gtatggttac tgacttcgtc agtttcatct acaacctcaa
    20941 aaccatgttt tgagctgact tcgtcagttc tatccacaat ctcaaaacac tgttttgagc
    21001 aacctgcggc taacttccta gtttgctctt tgattttcat tgagtattag tcctttttca
    21061 aacttcctgc acgagtttga gttcctgcat aggcaagtaa gagaagagtt cctgcaatag
    21121 ctacagatac accattggca attcccgcaa caatcccttg tgcaaatact ttttctgccg
    21181 cttcttgata aatcacaaca tctccaagtg gtgccaagac accccaaaca agggcatttg
    21241 caagtagttg aatgaggtta aaaataagaa tatctttcca gtcaaaaaca ccattgatca
    21301 ctcgaacgta ctttctaaaa agtcccacaa ctaaaccaaa gagtccgcta gcgataatcc
    21361 aagtccacca tagaccataa ccagcaagag agtccttgat tgcatgacca atcaacccga
    21421 caagcaaacc gataatcggt ccaaaaataa tagaaagtag cgcttgtacc gcatactgaa
    21481 gctggatgct tgtatttgga acaggggttg gaatgttgat catcccgatg acgacaaaga
    21541 gggcagcgcc aattccgaca gcaacaactt gtttaattgt aaatttgatt tccatactat
    21601 tctcctattt tatccttcta ttttctttat ttcaatggtc caagatgaac cgacacctac
    21661 attataggcc ttggcaaagg aaccttggtt gatagccaaa cctaaacgat agagagagtt
    21721 gatgtaaagg atgggttgcc caattctcac atctgcaaat gatttgccat agacaacctg
    21781 attttgatag accagcatat cagcatgata gatggtcact tcaaaacgat caccaaattc
    21841 tggttccagc ttgtaaaatt cttcccgtgt gatagaggtc caaagcgaac cgaaacgcac
    21901 atccagaata tcaatggctc ccttcaccag atgatcttct atgatggtcg ctacgactgg
    21961 aagctctaca atctggtcca cactgagctc tggccctact tcctcaaaag taatgtgacc
    22021 actggccagt ttagcaccag tataggcata gacatcacga ccgtggaagg tataagaatg
    22081 ctctgtgttt tgacgcctat tggccacctc agaaatctca cgaatggcta caatgccaac
    22141 gtgtttcttg ataaaggaaa gcgtcccatt atctggcgtg acaatgtatt gattttttgc
    22201 agtcttggca actacactct tacgtttcga accgacacct ggatcgacaa ccgatacaaa
    22261 cgtcgttccc tcaggccagt aatccaccgt ctgaaagaga cgatagctcc cctcaaaaat
    22321 attataaggc gtgatatcgt gcgtcaagtg atggattttt aaggttggag actcttctaa
    22381 agccactcca atcatagccg ataccgcacc atcaaccaga ccaaagtctg attgtaatac
    22441 cagtaaatta ttattcattt tatctccttg tatatccttt atcataccat atttcagaga
    22501 aagtcgctaa tagctatttc aattggttag ggtatagttt taccttatag cgaattttca
    22561 ctaaaagctc ttgttattag ctagtaggtg cattttttct tgaaaaaggg tatgatagtt
    22621 acatcatgaa aaagtaggtt ttattatgaa aattatcctt gtcggagggg gaaaagttgg
    22681 ttttgccctc tgtcgctcct tggttgcaga aaagcatgat gttttgctga ttgagcaaga
    22741 cgaagctgtt ctcaatcata ttgtcagtcg ctttgatatc attggtatcc ttggtaacgg
    22801 ggccgatttt gccattcttg agcaagccag cgtccaagat tgtgatatct ttatcgccct
    22861 gactgagcac gatgaagtca acatgattgc agcagttcta gccaagaaaa tgggagctaa
    22921 agaaactatc gttcgggtga ggaaccctga atattctaac tcttatttca aggaaaagaa
    22981 tattctcggt ttttctctta tcgttaatcc tgagctcttg gctgcccgcg ctatcgcgaa
    23041 tatcattgac ttccccaacg ccctgtctgt cgaacgcttt gctggtggac gcgttagcct
    23101 catggaattt gtcgtcaagt ccaccagcgg tctttgccaa atgcccattt ctgattttcg
    23161 taaaaaattt ggtaatgtca ttgtctgtgc gatagagagg gatcatcaaa ttatcattcc
    23221 aagcggtgac atgactgtac aggataaaga tagaatcttt gtcactggta accgtgtcga
    23281 tatgatactc ttccataatt attttaaatc acgcgccgtg aagagccttc tcatcgttgg
    23341 agcaggtaga attgcctatt atctacttgg tattctcaaa gatagtcgta tcgatacaaa
    23401 agtcattgaa atcaatcctg aaatcgccag cttctttagc gagaaattcc caaatctcta
    23461 catcgttcaa ggagatggaa ccgcaaaaga tatcctgctg gaagaaagtg ctcaacacta
    23521 tgatgccgtt gcgactctaa caggagtcga tgaggaaaat ctgattacat ctatgttcct
    23581 tgacagggta ggtgtacaga aaaatattac taaggtcaat cgtaccagtc tcctcgagat
    23641 tatcaatgcg cctgattttt caagtatcat cacacctaaa agcatcgctg tagatacgat
    23701 tatgcacttt attcgtggtc gagttaatgc ccagtattca gaccttcaag ccatgcacca
    23761 tctagccaat ggccaaatcg aaaccctgca attccatatc aaggaagcca ataaaatgac
    23821 tgccaaacct ctttctcaac tgaaattgaa aaaaggggtt cttattgcag ccattattcg
    23881 aaagggcaag actattttcc caactgggga ggatatgttg gaagttggag acaagctcct
    23941 agtaacaacc ttgttgccaa acatcaccaa gatttatgac ttgattgcga ggtaagaaat
    24001 gaataaaagt atgattcgtt acctcctttc aaagttactt ttgattgaag ctgttctcct
    24061 cttggttcct gtggctatcg ctgtctatta ccgtgaatcg agccaagtct ttacagccct
    24121 cttttcgacc atagggattc tcgtattgtt aggcggttca gggagtttac agaagccaaa
    24181 aaatcaacgg atttatgcca aggagggagt cttgatcgtt gccctctgtt ggatcctttg
    24241 gtctttcttt ggtggtctcc cctttgtctt ttctgggcaa atccctagcc ttattgatgc
    24301 cttttttgaa attagttctg ggtttacaac tactggagca agtattttga acgacatttc
    24361 ggttctcagc cgttccctcc tcttctggcg aagttttacc cacttgattg gagggatggg
    24421 agtgcttgtt tttgcacttg ctattatgga caatgccaaa aatagtcacc tagaagtaat
    24481 gaaggctgag gttcctggtc ctgtctttgg taaggttgta tccaaattaa aaaacactgc
    24541 ccagatcctc tatctccttt acatagccct cttttccctc tttgtcatca tctattatct
    24601 agccggtatg cctctctacg atagttttgt cattgctatg gggacagcgg gaactggagg
    24661 atttaccgtc tataacgatg gaattgccca ctatggtagc tcactgatta cctatctggt
    24721 tagtatagga gttttggttt ttggggtaaa tttcaatctc tactactacc ttatgctccg
    24781 tcgggttaag gccttctttg gagatgaaga gcttcgggct tacttggtca tcgtgctggt
    24841 ttctacaggc ttgattagcc tcaatactct ctacctctac ccagggtttt caaagagctt
    24901 tgaaatgacc ttcttccagg tttccaacat cattacaaca actggttttg gatatggaga
    24961 tattaccaac tggcccctct tctcccagtt tatcctcctt ttcctcatgg caatcggtgg
    25021 ctctgctgga tcaactgcag gtggactcaa gattattcga ggcctcatcc tttcaaaaat
    25081 tgccaaaaac caaattttgt caatcctatc gccccaccgt gttttgaccc tccatgttaa
    25141 taaaatggtg attgacaaag atacccagca taagattctc aagtactttg tcatctatgc
    25201 tatgattttg ctagcgctta tctttattgt cagcctagat agcaatgatt ttctggtcgt
    25261 gaccagcgct gtctttagct gtttcaataa tatcgggcct attctaggaa ccacttctag
    25321 tttctcaatc tttagtccca tctcaaaaat tctcctctcc tttgcaatga ttgcagggcg
    25381 cttggagatt tacccaatcc tccttctctt tatgaaaaga acttggtcta agagataaaa
    25441 tacaaaatcc ctctttttac aattgtgaaa gagggatttt tatattaaaa agggggaacg
    25501 tttttaaatc caagcctgtc tgtaagcaaa gttcttactc aaataaaaga tggaaaaact
    25561 tactacacag cttggtaatg ctccaataca aattgtaagg ctttacttgg attctttgtt
    25621 aaatgaatat attccattcc tctggtagca actatctgaa tacctaatct ttctgcatcc
    25681 accttcatct ctctataata actccgaact tgtggagcca gaataattaa atcataaaca
    25741 cccataatat cataatgagc tccgtacgct cctgaattcg caatcactct aacttctgct
    25801 aattgagcgc cctcgttaat tgcattggct aattacgcac ttgttccaga ccctgcacaa
    25861 agaaccagta ctttcaactc cttacttttt attacatcta tctcaccagg cattatttca
    25921 cttgtatcct cctctaaaat aatatcattt gagcttgcaa tatctataac ctgtagtaaa
    25981 tatctcgtat ttagacaata tgaaaacaag acgacttcca tataggaaac cgccttctcg
    26041 ctatgttgag tgatttatat taaaataact tttcttctag ctgcatttta ttattataaa
    26101 aacattcatc ataaccccca gaacttaaat aacaattttt attcaagata catactccta
    26161 gaataaactt tatatgaaat tctcattttt gtttttacaa ttctccttag ttaaatcttg
    26221 tttaatatat gttttacata tagtatttag cgcgacataa tactgaagtc tctctccaaa
    26281 aaacggttat gcttctttga ataggacgtt ctcacaagaa aagcatcttc acgtttcaac
    26341 ttcatatggc tcaaaaacaa tcaattgatg ctaaaacctg tacctagatg tttcggttca
    26401 taaaaccatg aaactgtaaa agtggatgaa attgatagcg atagtcaaat caagaggcat
    26461 cataactcta aaaagtcaca atatataagt tcatcctcgg aaaaatatca ttctaattgt
    26521 tgaaatggat atataaacag gggtattaac attaaatgct catgaaacaa cgaatacagg
    26581 tatcaaaact atgacaaaac aagtccctaa atttactaaa gacactgctc aactttacac
    26641 ctgtaaatgg ttgttgtata ataaagttac aaagatgtac gaccacactg ttgtaaatca
    26701 tagtgttcgc gaatatatta ctgatagcat ttctacaaat acaattgaag aggctggata
    26761 ggctttaaac gaaatatgtc agtgttttgg cattcctagc cttaatatca tttaaagaat
    26821 tgtatagata aaattttttc caatacagag actcgtaaca actgcttcat ttttctacca
    26881 acatatttag gaacaggata agatacaaga ggattaatcc ataaatcagt tctataccaa
    26941 tctaagataa ataagctaaa aaacaattga taataagcaa atagattcca aattttctct
    27001 atctgctcat tttaataaac aatactagtg taactatcct tccagtcaga agcttgtcaa
    27061 atcacaccga aaattcttct aaaatttatc tcgttaggca atcaagcaaa aactcgacga
    27121 tagtacaaac attatcatac aggattgact tcctaaatta tatactttag taaggttttc
    27181 gtataagaaa aaagattcat tttacatttc taaacattct tttctaagat gaaaaacaga
    27241 atttttcgat tgtgatttaa agcaacaaga agattttcag tatcatccta tagatacgag
    27301 ctaattaaga aaaactacat tttcgaatat aaactacaat aatataaact aaattttata
    27361 ggaggaagac aatggattgg tacgattata tgatacaggc atccaaacaa tcacaattca
    27421 acgcaagcca ttggtttcgc tatttgcgaa aagttatttt tgaagactat tcttatttaa
    27481 caaaccaaga tgtagaaaag ttgctagact ccaaagaact aacccgtttt caaaaaatta
    27541 gcttgaagta tgcctttcaa gagcatactc caactcataa atatgtgatt tcattaaata
    27601 aacctgctaa gttaaccaat gttcaaaaat tgatggagaa atacaaacat ggataaaatg
    27661 aaaccggtct tccaagccct aaataaggaa ttaattcagg aaaatctgac tttaacaatt
    27721 atctgtgtcg atggttatgt cttagaatat catggtttac gtgccacaca agatgttgat
    27781 gctttttatg accaaaatca gaaaatcaat gaaattattg ctcgtgtagg aaaacagttt
    27841 aatcttaaca tccacgaaga gttatggctc aacaatcatg ttgcaaagca aatatgaaca
    27901 aacaacctcc cttaagttta tgcgaatcct tgtactcatt tgagaatctg accgttttag
    27961 tagtccctat tgagtatgtg ttaggaatga agatgatgag tatcagagag caggatttgc
    28021 aagatattgg tgcaattatt aaatacaaaa actttcattc accattcgat acctttaaat
    28081 atttaaaaga catgggattt gatactattg acctctctgt tctattagaa ggttttagtt
    28141 atgcttatgg tatggattgg ttagaaaaat tttttaaaga aaatcaagat aaacttcgcg
    28201 aattttatta acagtatcaa acaagatgat tgtcaattag ccagtcgtct tgttttttat
    28261 gatgaaatga aataaaatct gaacaaatcg cttagagaat tccagtcaat ttctagcgct
    28321 gtttgagacg tttcagtgga ctatagtaaa atgaaataag aataggacaa atcgatcagg
    28381 acagtcaaat cgatttctaa gaatgtttta gaaataaagg tgtactattc tagtttcaat
    28441 ctattatatt tcagattcgg tccactattc cttcccaaaa acattgcata ttgcgccgaa
    28501 ggaattgctc atactatccc aagcacaaaa ccctcagtcg tttgaccgag ggttcctttt
    28561 tattattcaa gaacttgatt agctcaatgc atccaaggcg tcatccattg aaagaacttc
    28621 gtggaagaca cgttgtgtca attcagtttt ttgttctgga gtgaggtatt tagtgtttac
    28681 acagtatcca gagatacgta cgataacgtc ttcgcctgac atgatttttt cgtaaacatc
    28741 gttcaagtcc ataacgttca agttaacgtg ttgtccaccg ttttcgaagt aaccatcaag
    28801 gattgttacc aagttatcaa cttgttcatc acgagtctta ccaagagcgc gaggtgatac
    28861 ttgtgtagtc aatgagatac cgtcagctgc ataactaaag tcaaggctag aaagtgagtt
    28921 caagttttgc aaccaaccac ctttagcttt gttagatggg ttagcacctg gtgagaagaa
    28981 ttcaagttta gacaagttca cagaaccatc ttcgttgagg tatacacctt tgtgaactgg
    29041 tgagttacca gtttgtttag agtaagcaac gttagatgtg attgtcaaaa gtgatactgt
    29101 agcttctgcg tctttgtata gtttgtggct acgtagacga gttgtgtaag cttcgatcaa
    29161 ccattctgcc aattcgtttg aacgtgggtc atcttcaccc cagcgtgggt agtcaccgat
    29221 tgtttcgtaa tcgtagatgt agccatcttc gtcacggatt ggtttaactg tagcgtattt
    29281 gatagctgac aatgtatcaa cagtgttagc aaatccacag ataccgaatc ccatgttggc
    29341 acgttgttta gttggcaaga aggccatttg aacagcttcg tagttgtacc tatcagtcat
    29401 gtagtggatg atgttcaagg catctacgta agtgtcagtc aaccagtcaa gagatttttc
    29461 aaagttcgct ttaactgatt cgaattcaag aacttcgtca cggattggtt cgatatcaaa
    29521 tactttgtag tctttgtgaa catcgtcgta accaccattc aaaccagtaa gaagggcttt
    29581 aagaacgttt acacgagcac cgaagtactg gatgttgtgg cgttgttctt cattttctgg
    29641 atcaagtgga gacacacagc atgagataca gctcatttca ccatatccgt ctttagccat
    29701 tgttgttaca ccttcgtatt ggatagaaga gtgtttgtgg ctcatgtgca tacagtagcg
    29761 acggaagttg tatggcaatt tgtcagtcca aagaactgtc aagtttggtt ctggtgagtt
    29821 accgatgttg tcaagagtgt tcaagaaacg gtagtccatc ttagtaacac ggtgacgacc
    29881 gtcgttaccc ataccagcca tagaagttgt gataaaggtt gggtcacctg agtacaattg
    29941 gtcataagct tttgtacgag caaatttaac tgtacgaagt ttcataacga aatcatcaac
    30001 gaattcttgg atttctgatt cagtaaatgt accacgagca aggtcacgtt ctgcaaagat
    30061 gtccaatacg attggtacac gacctagaga tgtagcagca ccgttaatca cacggcagac
    30121 agccatgaaa gcaatgttaa cccattggat tgcttctttc acgttcatcg ctggtttgcg
    30181 aacatcaacc ccgtaaaggt cacccaggcg aacaacttgt tgcaatgctt ggtattgaag
    30241 gtttacttct tcacgaagac ggattgtttc ttcatcgatt tctttgattg cattccagtc
    30301 gtttactttt tcttgcatca agtagtctgc accgtaaaga gcaagacgtg cgtaaacacc
    30361 gatgatacgt ccgcgtgagt atgcatctgg aagaccagtt acagtgtgtg catgacgagc
    30421 gcgacgaata tttgaagtgt aggcacggaa aataccgtcg ttaactgttg ttacatattt
    30481 agtgaagatt tcgtgaacag ctgggtctgg ttcgtatcca ttttctttta aagtagtttc
    30541 agccatacgg ataccacctt ttggcatgaa gttcaatttg aagagttcat cattttggat
    30601 accaaagata acttcatttt ctttgtcgat aaatccagca gggatatcag cgatagatgt
    30661 tggacgagtg tccattggga aacgagtttc ttcgtagtgt gctttagttt cttctacaat
    30721 tttcttgatg tgaagtgaac gctctgttgg tcctgcaagg aagctttcgt ctccatcata
    30781 aggtgtgtag ttagcttgta caaagcgtga tacacttgct ttttctttcc aatctacgcc
    30841 tttgaagcct tcccaagctt tgtcaaaaat atcttgtgct tcaacaactg tcttaacaac
    30901 catattaatg tcctcttttt tctagtaaca gtcatctgtt acattcataa gacaagtata
    30961 ccataaagta accgatttca acaagtgcaa aatccctatt tttacacttt cttttctaaa
    31021 acagtctata ttttatttca aactgtatta tatttttgaa aaaaataaag tccttttttc
    31081 tttttttcag aaaaaagggt ataataaaag aaaataagca gtaacactca atggaaatcg
    31141 aaaagcaaac taggaagcta gccgcagatt gctcaaaaca ctgttttgag gttgcagata
    31201 gagctgacgt ggtttgaaga gattttcgaa gagtataaaa aggtgctagg catgttgatt
    31261 tttcctttgt taaatgattt gtcaagaaaa atcatccata ttgacatgga tgcctttttt
    31321 gctgcggtgg aaatcaagga taatcctaaa ctcagaggaa aacctgtcat tattggaagc
    31381 gaccctcggc aaacaggtgg acggggagtc gtttctacct gtagctatga ggctcgagct
    31441 tttggtgtcc attctgccat gagttccaag gaagcttatg aacgttgtcc ccaggccgtc
    31501 tttatctcag gaaattatga gaaatacaaa gctgtgggac tccagattcg agctattttt
    31561 aagcgctata cagatttgat tgaacccatg agcattgacg aagcctattt ggatgtgaca
    31621 gaaaataaac tcggtatcaa gtcagcggtc aaaattgctc gcctcattca aaaagatatc
    31681 tggcaagaac tccatctaac tgcttctgcc ggtatttcct acaacaagtt cttagctaaa
    31741 atggcgagtg attatcaaaa acctcatggt ttgacagtga ttctacctga acaggctgag
    31801 aattttctca aacaaatgga tatttccaaa tttcatggag taggaaaaaa gacagtagaa
    31861 cgtcttcatc aaatgggcgt ttttactggt gctgatttac ttgaagttcc tgaggtgacc
    31921 ctaatagacc gttttggtag actaggctat gatctgtatc gaaaggctcg tggcattcac
    31981 aactctccag tcaaatccaa tcgcatccgt aaatcaatcg gcaaggagaa aacctacggg
    32041 aagattctcc gtgctgagga agatatcaaa aaagagctga ctcttctatc agaaaaagtc
    32101 gctctcaatc tacatcaaca agaaaaagct ggaaaaattg tcattttgaa aatccgctac
    32161 gaggactttt caactcttac caaacgaaaa agtcttgctc aaaaaacaca agatgctagt
    32221 cagataagcc aaatagccct gcaactctat gaagaattaa gcgagaaaga aagaggtgtc
    32281 cgcctattgg ggattaccct gactggattt taaagctcaa tgaaaatcaa agagcaaact
    32341 aggaagctag ccgcaggctg ctcaaaacac tgttttgagg ttgcagataa agctgacgcg
    32401 gtttgaagag attttctaag agtataaaac cttgaagagt tgccccttca aggtttttct
    32461 tatacaaata aacggacaaa actgtaaagt agcaaaacac taccaagtac gatacggtat
    32521 ttaccaaaaa gggtgaagtc gtgttttttc acatagctgg tcaagaagcg aatagccacc
    32581 atgctgaccg caaaagctac tcccatcgcg accaagagca aaaacaattg cccaaagctc
    32641 aagagttctc cggctttcac aaatttgaaa atctttaagg cactagctcc aaacataacg
    32701 ggaatcccaa gatagaaggt aaattctgtc acaactgaac gactggttcc atttaacaaa
    32761 ccaccgacaa tcgttgcacc tgaacggcta gtccctggta aaagagcaag aacttggaag
    32821 agtccgatat agaaagcggt cgtataagga agcttgtcca actctgttac acttggctcg
    32881 atagcacgcg ctttattgcg cttttccaaa tagatgaagg caaccccgta gataatcaac
    32941 atgagagcaa ctgaaaccat gttatggaag tgggtatcaa accaatcatc aaatttaaag
    33001 acaccaagta aaggtaaagt agcaatcaag accttcaacc atagtctcca agtcttacga
    33061 acttcctgtt tgtccttagt cggtttaaaa ggattgagct tgttaaaata aatcaccata
    33121 actgctaaaa tagcaccaag ctgaatcacg acattaaaca tggacataaa ggcttcattt
    33181 tgattttggt attggatgaa ttcctctgct aaaatcaagt gacctgtact ggaaatcggc
    33241 aaccattccg taattccttc aacaatcccg aagaagatag attttaaaat ttcaataaga
    33301 tacatagatt actccctata ttataattag cgcagttagc tactgccaaa gcccaagtgg
    33361 tatacttgga ataagccact gtggattagt tcatttcctc caatcgcttg acgatttccg
    33421 aaaggatcta accacag
//
LOCUS       DBERDK010000018        32490 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.018, whole genome shotgun sequence.
ACCESSION   DBERDK010000018 DBERDK010000000
VERSION     DBERDK010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 32490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32490
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.018"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            585..1037
                     /locus_tag="ACL73L_000811"
     CDS             585..1037
                     /locus_tag="ACL73L_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HGS0771817.1"
                     /translation="MIITKISRLGTYVGVNPHFATLIDFLEKTGLENLTEGSIAIDGN
                     RLFGNCFTYLADGQAGDFFETHQKYLDIHLVLENEEAMAVTSPENVSVTQEYDEEKDI
                     ELYTGKVEQLVHLRAGECLITFPEDLHQPKVRINDEPVKKVVFKVAIS"
     gene            1056..2393
                     /locus_tag="ACL73L_000812"
     CDS             1056..2393
                     /locus_tag="ACL73L_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGS0771818.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEIT
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYSVPLYSHAQVMWV
                     RTDLLKEHNIEVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDKDQGIETSNIPMVVWKNSKHPEVAKAFL
                     EALYNEEDYVKFLDSTPVGMLPTIKGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQAGMLTNQHIIEQMFQDIITNGTDPMKAAKEAEKQLNDLFEAVQ"
     gene            2472..3356
                     /locus_tag="ACL73L_000813"
     CDS             2472..3356
                     /locus_tag="ACL73L_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGS0771819.1"
                     /translation="MVKERNLTRWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKAVLSDPNFFNAFFNSIKWTVFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWILNGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASAVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            3356..4189
                     /locus_tag="ACL73L_000814"
     CDS             3356..4189
                     /locus_tag="ACL73L_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGS0771820.1"
                     /translation="MKKKSSIYLDILSHVLLVGATIVAVFPLVWIIISSVKGKGELTQ
                     YPTRFWPEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLIAIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNKIASGLSEGSV
                     K"
     gene            4384..5487
                     /locus_tag="ACL73L_000815"
     CDS             4384..5487
                     /locus_tag="ACL73L_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HGS0771821.1"
                     /translation="MVKYGVVGAGYFGAELARYMQKNDGAEITLLYDPDNAEAIAEEL
                     GAKVASSLDELVSSDEVDCVIVATPNNLHKEPVIKAAQHGKNVFCEKPIALSYQDCRE
                     MVDACKENNVTFMAGHIMNFFNGVHHAKELINQGVIGDVLYCHTARNGWEEQQPSVSW
                     KKIREKSGGHLYHHIHELDCVQFLMGGMPETVTMTGGNVAHEGEHFGDEDDMIFVNME
                     FSNKRFALLEWGSAYRWGEHYVLIQGSKGAIRLDLFNCKGTLKLDGQESYFLIHESQE
                     EDDDRTRIYHSTEMDGAIAYGKPGKRTPLWLSSVIDKEMRYLHEIMEGAPVSEEFAKL
                     LTGEAALEAIATADACTQSMFEDRKVKLSEIVK"
     gene            5661..6359
                     /locus_tag="ACL73L_000816"
     CDS             5661..6359
                     /locus_tag="ACL73L_000816"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HGS0771822.1"
                     /translation="MPQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAA
                     EQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIE
                     VIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTL
                     SGYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIHTPEQAKQILEYGVRGIVVGGAIT
                     RPKEITERFVASLK"
     gene            6378..7895
                     /locus_tag="ACL73L_000817"
     CDS             6378..7895
                     /locus_tag="ACL73L_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HGS0771823.1"
                     /translation="MFKVLQKVGKAFMLPIAILPAAGLLLGIGGALSNPTTIATYPIL
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGSAIDTGVIGALVVGIVAVYLHNRYNNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHIIYPM
                     FWYTELGGVETVAGQTVVGAQKIFFAQLADLAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFFGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADVLNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPFGLIWSVLYYIIFRWFI
                     TQFNVLTPGRGEEVDSKEISESADSTSNTADYLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            8020..9348
                     /locus_tag="ACL73L_000818"
     CDS             8020..9348
                     /locus_tag="ACL73L_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGS0771824.1"
                     /translation="MKFRKLACTVLAGAAVLGLAACGNSGGSKDAAKSGGDGAKTEIT
                     WWAFPVFTQEKTGDGVGTYEKSIIEAFEKANPDIKVKLETIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDEFVKDVNNENIVQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATSWIKDNLINNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYEDKRMETISGWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            9600..10466
                     /locus_tag="ACL73L_000819"
     CDS             9600..10466
                     /locus_tag="ACL73L_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGS0771825.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAVMIAIVSF
                     VQFKVLGNDVEY"
     gene            10482..11321
                     /locus_tag="ACL73L_000820"
     CDS             10482..11321
                     /locus_tag="ACL73L_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGS0771826.1"
                     /translation="MQSTEKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPD
                     TIVIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTMFLVCATSSLAGYVLAK
                     KRFYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLM
                     KQFSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLV
                     MLTSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGA
                     VKG"
     gene            11593..12045
                     /locus_tag="ACL73L_000821"
     CDS             11593..12045
                     /locus_tag="ACL73L_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HGS0771827.1"
                     /translation="MIFDDLKNITFYKGIYPNLDKAIDYLYQHRKDSFELGKYDIDGD
                     KVFLVVQENVLNQAENDQFEYHKNYADLHLLIEGHEYSSYGSRIKDEAVAFDEASDIG
                     FVHCYEHYPLLLGYHNFAIFFPGEPHQPNGYAGMEEKVRKYLFKILID"
     gene            12142..12486
                     /locus_tag="ACL73L_000822"
     CDS             12142..12486
                     /locus_tag="ACL73L_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771828.1"
                     /translation="MVSSKYAARASFLDGQGITVDEMAWIIRGIVNALIGRYIKLGAY
                     AAKYGISMARSILSRAAATAAARVGLLTKIFGWILRIAVLAADVYGNFANNIAAAWDA
                     YDKIPNNGRINF"
     gene            12496..12690
                     /locus_tag="ACL73L_000823"
     CDS             12496..12690
                     /locus_tag="ACL73L_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771829.1"
                     /translation="MKALCIFLLNMLAWTVLAMIIRGGLDGFNEHTWSTILIASLSGI
                     YIDYMSIDKSRKKSKRKKKK"
     gene            12773..13417
                     /locus_tag="ACL73L_000824"
     CDS             12773..13417
                     /locus_tag="ACL73L_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HGS0771830.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVVSCLPIV
                     TIGVAKISLYETMFEVKKSRRVPVFKIYLRSFKQNLKLGLQLGLMELGIVFLTLSDLY
                     LFWGQTALPFQLLKAICLGILIFLTIVMLASYPIAARYDLSWKEILQKGLMLASFNFP
                     WFFLMLAILVLIVMVLYLSAFSLLLGGSVFLLFGFGLLVFIQTGLMEKIFAKYQ"
     gene            13586..14503
                     /locus_tag="ACL73L_000825"
     CDS             13586..14503
                     /locus_tag="ACL73L_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HGS0771831.1"
                     /translation="MSDLKKYEGVIPAFYACYDDQGEVSPERTRALVQYFIDKGVQGL
                     YVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGV
                     DAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEMLKN
                     PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGAKAGIGGTYGAMPE
                     LFLKLNQLIADKDLETARELQYAINAIIGKLTATHGNMYGVIKEVLKINEGLNIGSVR
                     SPLTPVTEEDRPVVEAAAALIRETKERFL"
     gene            14521..15405
                     /locus_tag="ACL73L_000826"
     CDS             14521..15405
                     /locus_tag="ACL73L_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HGS0771832.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGPVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFTI
                     PCEIENDVNCAGLAEAVSGSGKGASVTLCLTIGTGIGGCLIMDRKVFHGFSNSACEVG
                     YMHMQDGAFQDLASTTALVKYVAEAHGEDVDQWNGRRIFKEATEGNKICMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAHH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(15421..16272)
                     /locus_tag="ACL73L_000827"
     CDS             complement(15421..16272)
                     /locus_tag="ACL73L_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HGS0771833.1"
                     /translation="MDKPDIATIIDSHFEEMTDLEQEIARYFLQAETITDDLSSQQVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHQAENQANQVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEIQLERIAQLIEDAERIYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDENCLVLGFSLSGSTPSILDSLLDAKEMGAKTVLFTSVPNKDSQTYTET
                     VLVATHSQTSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
     gene            16472..18529
                     /gene="pbp2b"
                     /locus_tag="ACL73L_000828"
     CDS             16472..18529
                     /gene="pbp2b"
                     /locus_tag="ACL73L_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2B"
                     /protein_id="HGS0771834.1"
                     /translation="MRLICMRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNK
                     DFYEKKLASASQTKITSSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKETA
                     KKLLTYVSISSPNLTERQLADYYLADPEIYKKTVEALPSEKRLGSDGNRLSESELYNN
                     AVDSVQTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKEMP
                     GISISTSWDRKVLETSLSSIVGSVSSEKAGLPAEEAEAYLKKGYSLNDRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELENGGAKYSEGVYAVALNPKTGAVLSMSGIKHDLKTGELTPDSLGTVTNVFVPGSV
                     VKAATISSGWENGVLSGNQTLTDQSIVFQGSAPINSWYTQAYGSFPITAVQALEYSSN
                     TYMVQTALGLMGQTYQPNMFVGTSNLESAMEKLRSTFGEYGLGTATGIDLPDESTGFV
                     PKEYSFANYITNAFGQFDNYTPMQLAQYVATIANNGVRVAPRIVEGIYGNNDKGGLGD
                     LIQQLQPTEINKVNISDSDMSILHQGFYQVAHGTSGLTTGRAFSNGALVSISGKTGTA
                     ESYVADGQQATNTNAVAYAPSDNPQIAVAVVFPHNTNLTNGVGPSIARDIINLYQKYH
                     PMN"
     gene            18540..19136
                     /gene="recR"
                     /locus_tag="ACL73L_000829"
     CDS             18540..19136
                     /gene="recR"
                     /locus_tag="ACL73L_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HGS0771835.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSADDVNEFA
                     KNLLSAKRELTYCSICGRLTDDDPCSICTDSTRDQTTILVLEDSRDVAAMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMDSEVSEVIVATNATADGEATSMYLSRLL
                     KPAGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            19312..20355
                     /locus_tag="ACL73L_000830"
     CDS             19312..20355
                     /locus_tag="ACL73L_000830"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HGS0771836.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMRAVNYDRFTVKTFFISQ
                     SGDSIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLAYPVFTKPSNMGSSVGISKSENQEELRQALKLAFRYDSRVLVEQGVNAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKVTMDIPAKISDDVVAVMRQNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWENMGISYPELIERLVDL
                     AKESFDKREAHLI"
     gene            20439..21812
                     /gene="murF"
                     /locus_tag="ACL73L_000831"
     CDS             20439..21812
                     /gene="murF"
                     /locus_tag="ACL73L_000831"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HGS0771837.1"
                     /translation="MKLTIHEIAQVVGAKNDISIFEDTQLEKAEFDSRLIGTGDLFVP
                     LKGARDGHDFIETAFENGAAVTLSEKEVSNHPYILVDDVLTAFQSLASYYLEKTTVDV
                     FAVTGSNGKTTTKDMLAHLLSTRYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLAP
                     ADPIVEDYLPIDKKVVRFGQGAELEITDLVERKDSLTFKANFLEQALDLPVTGKYNAT
                     NAMIASYVALQEGVSEEQIRLAFQHLELTRNRTEWKKAANGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGDQSVQLHNQMILSLSPDVLDIVIFYGEDIAQ
                     LAQLASQMFPIGHVYYFKKTEDQDQFEDLVKQVKESLGAYDQILLKGSNSMNLAKLVE
                     SLENECK"
     gene            21799..22410
                     /locus_tag="ACL73L_000832"
     CDS             21799..22410
                     /locus_tag="ACL73L_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HGS0771838.1"
                     /translation="MNASDFAKYLQRMIALTDTGLTFTKDPFDRERYEDLRSLLSEML
                     NQVSDLDADEVAELLKPTSAYATPLMDVRAWIVEDEKICLVRGQGENDWALPGGFGEV
                     GYSPTENILKEIEEETGFKAKVERLLAVFDTNRFQLQSKQYAKFVFECKLHDGQFQEN
                     QEIADLQFFAIDQLPNLSEKRITKEQIELLWQVYQGHRGQYLD"
     gene            22501..23205
                     /locus_tag="ACL73L_000833"
     CDS             22501..23205
                     /locus_tag="ACL73L_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HGS0771839.1"
                     /translation="MNLWDIFFTTQATEPPKFDLFWYVSLFTLLALTFYTAHRYRENK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGQSKYKQYFA
                     LLGTFGTLAAFVYPVPDAYPFPHITILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLVTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIAKEA"
     gene            23424..24797
                     /gene="ftsA"
                     /locus_tag="ACL73L_000834"
     CDS             23424..24797
                     /gene="ftsA"
                     /locus_tag="ACL73L_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HGS0771840.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKD
                     GIIVDIDAAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENVIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKEAFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVPNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVELVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            24814..26073
                     /gene="ftsZ"
                     /locus_tag="ACL73L_000835"
     CDS             24814..26073
                     /gene="ftsZ"
                     /locus_tag="ACL73L_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HGS0771841.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVAPQARSATN
                     YRETVKPAHSHGFDRHFDMAETAELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSV
                     VSPVERFEAPISQDEDELDTPPFFKNR"
     gene            26078..26749
                     /locus_tag="ACL73L_000836"
     CDS             26078..26749
                     /locus_tag="ACL73L_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HGS0771842.1"
                     /translation="MNVKENTELVFREVAEASLSANRERGSVSVIAVTKYVDVPTAEA
                     LLPLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDVIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELLEILPELARLDKIEYVGLMTMA
                     PFEASSEQLKEIFKATQDLQREIQEKQIPNMPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            26759..27298
                     /locus_tag="ACL73L_000837"
     CDS             26759..27298
                     /locus_tag="ACL73L_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HGS0771843.1"
                     /translation="MSLKDRFDRFIDYFTEDEDSSLPYEKRDEPVFTPVNSSQEPALP
                     MNQPSQSAGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDLLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            27298..27561
                     /locus_tag="ACL73L_000838"
     CDS             27298..27561
                     /locus_tag="ACL73L_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HGS0771844.1"
                     /translation="MIFLIRMIYNAVDIYSLILVAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSVWVAIVLVRFLGENLVRFLAMIG"
     gene            27558..28343
                     /locus_tag="ACL73L_000839"
     CDS             27558..28343
                     /locus_tag="ACL73L_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HGS0771845.1"
                     /translation="MNKGIYQHFSIEDRPFLDKGMEWIKKVEDSYAPFLTPFINPHQE
                     KLLKILAKTYGLACSSSGEFVSSEYVRVLLYPDYFQPEFSDFEISLQEIVYSNKFEHL
                     THAKILGTVINQLGIERKLFGDILVDEERAQIMINQQFLLLFQDGLKKIGRIPVSLEE
                     RPFTEKIDKLEQYRELDLSVSSFRLDVLLSNVLKLSRNQANQLIEKKLVQVNYHVVDK
                     SDYTVQVGDLISVRKFGRLRLLQDKGQTKKEKKKITVQLLLSK"
     gene            28352..29140
                     /gene="divIVA"
                     /locus_tag="ACL73L_000840"
     CDS             28352..29140
                     /gene="divIVA"
                     /locus_tag="ACL73L_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIVA"
                     /protein_id="HGS0771846.1"
                     /translation="MPITSLEIKDKTFGTRFRGFDPEEVDEFLDIVVRDYEDLVRANH
                     DKNLRIKSLEERLSYFDEIKDSLSQSVLIAQDTAERVKQAAHERSNNIIHQAEQDAQR
                     LLEEAKYKANEILRQATDNAKKVAVETEELKNKSRVFHQRLKSTIESQLAIVESSDWE
                     DILRPTATYLQTSDEAFKEVVSEVLGEPIPAPIEEEPIDMTRQFSQAEMEELQARIEV
                     ADKELSEFEAQIKQEVETPTPVVSPQVEEEPLLVQLAQCMKNQK"
     gene            29488..32280
                     /gene="ileS"
                     /locus_tag="ACL73L_000841"
     CDS             29488..32280
                     /gene="ileS"
                     /locus_tag="ACL73L_000841"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HGS0771847.1"
                     /translation="MKLKDTLNLGKTAFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     EGKPHFVLHDGPPYANGNIHVGHAMNHISKDIIIRSKSMSGFNSPYIPGWDTHGLPIE
                     QVLAKQGVKRKEMDLVEYLKLCREYALSQVYKQRDDFKRLGMSGDWENLYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GVLDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPAGETRKFVVAAELLTSLSEK
                     FGWVDVQVLATYRGQELNHIVTEHPWDTAVDELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANNLEVAVTVDERGIMMKNAGPEFEGQFYEKVVPTVIEKLGNLLLVQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDAIDKVKFHTEWGKVRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMTAETIEHVAQLFEVHGSSIWWERDAKDLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVLVNRPNLTYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFALDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPAQDVVAYDKLRSVDKYMT
                     IRFNQLVKTIRDAYADFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFETEDFVQLSELPEVQTFANQEEILD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SELTIAEGPAPEAALSFEDVAFTVERAAGEVCDRCRRIDPTTAERSYQVVICDHCASI
                     VEENFVEAVAEGFEEK"
BASE COUNT         9902 a         5795 c         7096 g         9697 t
ORIGIN      
        1 aaagtatact ctaattgaaa acggtaacaa aactaattta gagaatgaaa tatagagtat
       61 ttctctctta aaagtttttg gtgaaacgag atgtagaaag gagatttagc caaagagtct
      121 attagtgcta gaataataga ttagaattat tttagaaaaa cgaagtgagc agcttataaa
      181 ttcaagtccc caaatagact catactagta tcttttgcaa aaaataaagg gcgacttcct
      241 tcatgaatat caatttcatc tataaggaag gtagctaatt gaactaactt atttattctg
      301 tttgtcgcta gaaaaatcag acctccttgt gaagattgag gagatactta atgaaaatca
      361 aagaagaaac tagcaagcta gtagcagatt gcccaaaaca ccgctttgag gttgtagata
      421 agactgacct atataatcca aggtgaagcg actgtggttt gaagagattt tcaaagagta
      481 tagactagag agtagtgttt ttatgtcctt ctagtagaaa atgctagaca gaagaatggg
      541 gaacttggat aggaaaaata gattgagaaa ggaggttaga agagatgatt attacaaaaa
      601 ttagccgttt aggaacttat gtgggagtca atccacattt tgcaacatta atagattttc
      661 tagaaaaaac aggactagaa aatttaacag aaggttcgat tgctatcgat ggtaatcgat
      721 tgtttgggaa ttgctttact tatctagcag atggtcaagc aggggatttc tttgaaacac
      781 accaaaaata tttggatatt catttagttt tggaaaacga agaagccatg gctgttacat
      841 cgccggaaaa tgtaagcgtt acccaagaat atgatgaaga gaaagatatt gaattataca
      901 cagggaaagt ggaacagttg gttcatttga gagctggtga atgcctcatc acttttccag
      961 aagatttaca tcaacccaag gttcgtataa atgatgaacc tgtgaaaaaa gttgtcttta
     1021 aagttgcgat ttcttaatgt agaaagagaa gaacgatgaa aaaaatgaga aagtttttat
     1081 gtctagctgg aattgcgcta gcggctgttg ccttggtagc ttgttcagga aaaaaagaag
     1141 ctacaactag tactgaacca ccaacagaat tatctggtga gattacaatg tggcactcct
     1201 ttactcaagg accccgttta gaaagtattc aaaaatcagc agatgctttc atgcaaaagc
     1261 atccaaaaac gaaaatcaag attgaaacat tttcttggaa tgacttctat actaaatgga
     1321 ctacaggttt agcaaatgga aatgtgccag atatcagtac agctcttcct aaccaagtaa
     1381 tggaaatggt caactcagac gctttggttc cgctaaatga ttctatcaag cgtattggac
     1441 aagataaatt taacgaaact gccttaaatg aagcaaaaat cggagatgat tactactctg
     1501 ttcctcttta ttcacatgca caagtcatgt gggttagaac agatttgtta aaagaacata
     1561 atattgaggt tcctaaaact tgggatcaac tctatgaagc ttctaaaaaa ttgaaagaag
     1621 ctggagttta tggcttgtct gttccgtttg gaacaaatga cttaatggca acacgtttct
     1681 tgaacttcta cgtacgtagt ggtggaggaa gcctcttaac aaaagatctt aaagcagact
     1741 tgacaagcca acttgctcaa gatggtatta aatactgggt taaattgtat aaagaaatct
     1801 cacctcaaga ttctttgaac tttaatgtcc ttcaacaagc taccttgttc tatcaaggaa
     1861 aaacagcatt tgactttaac tctggcttcc atatcggagg aattaatgcc aacagtcctc
     1921 aattgattga ttcgattgat gcttatccta ttccaaaaat caaagagtct gataaagatc
     1981 aaggaattga aacctcaaac attccaatgg ttgtttggaa aaattcaaaa catccagaag
     2041 ttgctaaagc attcttagaa gcactttata atgaagaaga ctacgttaaa ttccttgatt
     2101 caactccagt aggtatgttg ccaactatta aggggattag cgattctgca gcctataaag
     2161 aaaatgaaac tcgtaagaaa tttaaacatg ctgaagaagt aattactgaa gctgttaaaa
     2221 aaggtactgc tattggttat gaaaatggac caagtgtaca agctggtatg ttaactaacc
     2281 aacacattat tgaacaaatg ttccaagata tcattacaaa tggaacagat cctatgaaag
     2341 cagcaaaaga agcagaaaaa caattaaatg atttatttga ggctgttcag tagatgtaaa
     2401 agactagaaa ataggtgggc tagtgagctg aaaagctcta gcccaatctt gtaaaagaag
     2461 ggagaaggag aatggttaaa gaacgtaatt taactcgctg gatatttgtt ttgccagcta
     2521 tgattatcgt aggattactc tttgtttatc cgtttttctc gagtattttt tatagcttta
     2581 ccaataagca tttgattatg cctaattata aatttgttgg tttggctaac tataaagctg
     2641 tgctatcaga tcccaacttc tttaatgcgt tctttaattc aattaagtgg accgttttct
     2701 cattagttgg tcaagtttta gtagggtttg tattggcttt agctcttcac agagtacgcc
     2761 acttcaagaa attatatagg accttattga ttgttccttg ggcatttcct accatcgtta
     2821 ttgccttttc ttggcagtgg attctaaacg gggtttatgg ctacttacct aatctaatcg
     2881 taaaattagg tttaatggaa catacacctg catttttgac agatagtaca tgggcattcc
     2941 tatgtttggt gtttatcaac atttggtttg gagcaccaat gattatggtt aatgtgcttt
     3001 cagctttgca aacagtacca gaagaacaat ttgaggctgc taagatagat ggtgcttcaa
     3061 gttggcaggt gttcaagttt atcgtctttc cacatattaa agtggttgta ggacttctag
     3121 ttgttttgag aactgtatgg atctttaata actttgacat tatctacctc attactggtg
     3181 gtggaccagc caatgctaca acgacgcttc caatttttgc ttacaacctg ggctggggaa
     3241 ctaaattgtt gggtcgtgct tcagcagtta cagtattgct ctttatcttc ttggtggcga
     3301 tttgctttat ctactttgct atcatcagta agtgggaaaa ggagggtaga aaataatgaa
     3361 gaagaaatcc agtatttatt tagatattct ctcacatgta cttttagttg gtgcgaccat
     3421 cgttgcagtt ttcccattgg tatggattat catatcttct gtcaaaggga aaggggaatt
     3481 aactcagtat ccaacacgat tttggcctga acagtttaca ttagattatt tcactcatgt
     3541 tatcaacgat ttgcacttca ttgataacat tcgaaacagt ttaatcattg ccttggctac
     3601 aacccttatt gcgattatta tttctgctat ggcagcctat ggtattgttc gattctttcc
     3661 taaattggga gcaatcatgt cgagactact cgtcattacc tacattttcc caccaatttt
     3721 gttagcaatt ccctattcaa ttgccattgc taaagttggg ttaacaaata gtttatttgg
     3781 cttgatgatg gtttatctat cttttagtgt tccatatgca gtttggctct tagttggatt
     3841 tttccaaaca gttccaattg gaattgaaga agcggctaga attgatggtg caaataaatt
     3901 tgttacgttt tataaagttg tgctaccgat tgtagcacca ggtattgtag caacagctat
     3961 ttatacattt atcaatgctt ggaatgaatt cctgtatgcc ttgattttga ttaacaatac
     4021 aggaaagatg acagtagcag tagcccttcg ttcacttaat ggttcagaaa tactagactg
     4081 gggagatatg atggcagcgt ctgttattgt agttcttcca tcaattattt tcttctctat
     4141 catccaaaat aagattgcaa gtggattatc agaaggatct gtgaagtaat actcaatgaa
     4201 aatcaaagag caaactagga aactagccgc aggctgctca aagtacagct ttgaggttgc
     4261 agataaaact gacgaagtca gtaaccatac gcacagtaag gcgacgttga cgcggtttga
     4321 agagattttc gaagagtata agttaaaatt agaaagttta ttcaataata aaggagaaaa
     4381 aacatggtta aatacggtgt tgttggagca gggtattttg gagctgaatt ggctcgctac
     4441 atgcaaaaaa atgatggagc agagattact cttctctatg atccagataa tgcagaggcg
     4501 attgcagaag aattgggagc aaaagtagca agttccttag atgagttggt ttctagcgat
     4561 gaagtagatt gtgttatcgt cgcaactcca aataatcttc ataaggaacc ggttattaag
     4621 gctgcacagc atggtaaaaa tgttttctgt gaaaaaccaa ttgcgctttc ttatcaagat
     4681 tgtcgcgaga tggtagatgc gtgtaaagaa aacaatgtaa cctttatggc aggacatatt
     4741 atgaatttct ttaatggtgt tcatcatgca aaagaactca tcaatcaagg agttatcgga
     4801 gacgttctat attgtcatac agctcgtaat ggttgggaag aacaacaacc gtcagtgtca
     4861 tggaaaaaaa ttcgtgaaaa atcaggtggt cacttgtatc accacatcca tgaattggat
     4921 tgcgttcaat tccttatggg gggcatgcct gaaactgtaa ccatgacagg tggaaatgtg
     4981 gcccatgaag gtgaacattt cggtgatgaa gatgatatga tttttgtcaa tatggaattt
     5041 tctaataagc gttttgcctt gttagaatgg ggttcagctt atcgttgggg tgaacattat
     5101 gtcttaatcc aaggaagcaa aggtgccatc cgcttagact tattcaactg taaaggaact
     5161 cttaagctag atgggcaaga aagctatttc ttgattcacg aatcgcaaga agaagatgat
     5221 gatcggactc gtatctatca tagtacagag atggatggag caattgctta tggtaaacca
     5281 ggtaaacgta ctccattatg gctatcatct gtcattgata aagaaatgcg ctatctgcat
     5341 gagattatgg aaggagcccc agtatcagaa gaatttgcaa aacttttgac aggtgaagct
     5401 gccctagaag caattgctac tgcagatgct tgtacccagt ctatgtttga agatcgcaaa
     5461 gtaaaattgt cagaaattgt aaaataaatt ttggtattct cctatttata ggtcgacttg
     5521 ctcctctgaa agtactttta gaggagctgt ttgactttgc tagtttttga aactgaaatc
     5581 tattatacta caaactattg aaagcgtttt aattttaagg tataataatc tcatagaaat
     5641 aaagaaaagg aggaaagagg atgccacaga ttagcaaaga agccttgatt gagcaaatca
     5701 aagatggaat catcgtttct tgtcaggctc ttcctcatga accgctttat acagaagcgg
     5761 gaggggtgat tcccttgctg gtcaaagcgg ctgagcaagg tggagcagtc ggtatccgag
     5821 caaacagtgt tcgcgatatc aaggaaatta aggaagtcac taaacttcca atcattggga
     5881 ttatcaaacg tgattatcca cctcaggaac ccttcatcac ggctactatg aaagaagttg
     5941 atgaattggc agaactggac atcgaggtga ttgctctgga ttgtaccaag cgtgaacgct
     6001 acgatggttt ggaaattcaa gagttcattc gtcaggttaa ggagaaatat cctaatcagc
     6061 ttttgatggc tgatactagt atcttcgaag aagggctagc agctgtagaa gcaggaattg
     6121 actttgtcgg aacaacctta tcaggctaca catcctacag tccaaaagta gacggtccag
     6181 attttgaatt gattaagaaa ctctgtgatg ccggtgtaga tgtcattgca gaaggaaaaa
     6241 ttcatacacc agaacaagcc aaacaaatcc ttgaatatgg agtgcgaggc atcgttgttg
     6301 gtggcgccat tactagacca aaagagatta cagaacgctt cgttgctagt cttaaataag
     6361 atgtgagagg gagttttatg tttaaagttt tacaaaaagt tggaaaagct tttatgttac
     6421 ctatagctat acttcctgca gcaggtctac ttttggggat tggtggtgca ctttcaaacc
     6481 caaccacgat agcaacttat ccaatactag acaatagtat ttttcaatca atattccaag
     6541 taatgagctc tgcaggagag gttgtattca gtaatttgtc actacttctc tgtgtgggat
     6601 tatgtattgg cttagcgaaa cgagataaag gaaccgctgc gttagcagga gtaactggtt
     6661 acttagttat gactgcaacg atcaaagctt tggtaaaact ttttatggca gaaggatctg
     6721 caattgatac tggagttatt ggagcattag ttgtcggaat agttgccgta tatttgcaca
     6781 accgatataa caatattcaa ttaccttccg ctttaggatt ctttggaggt tcacgcttcg
     6841 ttcctattgt tacatcgttc tcttctatct tgattggctt tgtcttcttt gttatttggc
     6901 cacctttcca acaacttctt gtttctacag gtggatatat ttctcaggcg ggtccaattg
     6961 gaacttttct atatggattt ttaatgagac tttctggagc agtaggctta catcatataa
     7021 tttaccctat gttttggtat actgaacttg gtggtgttga aactgttgca ggacaaacag
     7081 tggttggagc tcaaaaaata ttttttgctc aattagccga tttggcccat tctggattat
     7141 ttacagaagg aacaaggttt tttgcaggtc gtttctcaac aatgatgttc ggtttaccgg
     7201 ctgcctgttt agcgatgtac catagtgttc ctaaaaatcg tcgtaaaaaa tacgcgggtt
     7261 tgttttttgg agttgcttta acatctttta ttaccggtat tacagaacca attgaattta
     7321 tgtttctatt cgtcagtccg gttctatatg ttgttcacgc attccttgat ggtgttagct
     7381 tctttattgc agacgtctta aatatttcaa taggaaacac attttcagga ggtgtaatcg
     7441 atttcacttt atttggaatt ttgcagggga acgctaagac gaattgggtt cttcagattc
     7501 catttggact tatttggagt gttttgtatt atattatttt tagatggttc attactcaat
     7561 tcaacgttct aacgccagga cgaggagaag aagtagattc taaagaaatt tctgaatccg
     7621 cagattcaac ttcaaatact gcagattatt taaaacagga tagcctacaa attatcagag
     7681 ccttgggtgg atcaaataat atagaagatg tagatgcttg tgtgacacgt ttacgtgtag
     7741 ctgtaaaaga agttaatcaa gttgataaag cacttttaaa acaaattggt gcagttgatg
     7801 tcttagaagt gaagggtggc attcaagcaa tatatggagc aaaagcaatc ttatataaaa
     7861 atagtattaa tgaaatttta ggtgtagatg attaagtact tactgactta ataaaaaaca
     7921 gaggagagtg atggatgagt aggatgaaat gaaatcgcat acaagaaata aagaactcat
     7981 tatccaagtt ggatacgctt attacatagg agaatacaaa tgaaatttag aaaattagct
     8041 tgtacagtac ttgcgggtgc tgcggttctt ggtcttgctg cttgtggcaa ttctggcgga
     8101 agtaaagatg ctgccaaatc aggtggtgac ggtgccaaaa cagaaatcac ttggtgggca
     8161 ttcccagtat ttacccaaga aaaaactggt gacggtgttg gaacttatga aaaatcaatc
     8221 atcgaagcgt ttgaaaaagc aaacccagat ataaaagtga aattggaaac catcgacttc
     8281 aagtcaggtc ctgaaaaaat cacaacagcc atcgaagcag gaacagctcc agacgtactc
     8341 tttgatgcac caggacgtat catccaatac ggtaaaaacg gtaaattggc tgagttgaac
     8401 gacctcttca cagatgaatt tgttaaagat gtcaacaatg aaaacatcgt acaagcaagt
     8461 aaagctggag acaaggctta tatgtatccg attagttctg ccccattcta catggcaatg
     8521 aacaagaaaa tgttagaaga tgctggagta gcaaaccttg taaaagaagg ttggacaact
     8581 gatgattttg aaaaagtatt gaaagcactt aaagacaagg gttacacacc aggttcattg
     8641 ttcagttctg gtcaaggggg agaccaagga acacgtgcct ttatctctaa cctttatagc
     8701 ggttctgtaa cagatgaaaa agttagcaaa tatacaactg atgatcctaa attcgtcaaa
     8761 ggtcttgaaa aagcaactag ctggattaaa gacaatttga tcaataatgg ttcacaattt
     8821 gacggtgggg cagatatcca aaactttgcc aacggtcaaa catcttacac aatcctttgg
     8881 gcaccagctc aaaatggtat ccaagctaaa cttttagaag caagtaaggt agaagtggta
     8941 gaagtaccat tcccatcaga cgaaggtaag ccagctcttg aataccttgt aaacggattt
     9001 gcagtattca acaataaaga cgacaagaaa gtcgctgcat ctaagaaatt catccagttt
     9061 atcgcagatg acaaagagtg gggacctaaa gacgtagttc gtacaggtgc tttcccagtc
     9121 cgtacttcat ttggaaaact ttatgaagac aaacgcatgg aaacaatcag cggctggact
     9181 caatactact caccatacta caacactatt gatggttttg ctgaaatgag aacactttgg
     9241 ttcccaatgt tgcaatctgt atcaaatggt gacgaaaaac cagcggatgc tttgaaagcc
     9301 ttcactgaaa aagcaaacga aacaatcaaa aaagctatga aacaatagtc cttagttatt
     9361 ctataaaaag tagtttttta aagaacctaa gagtgtatac ccccttttcc ctctacacag
     9421 atagtgtaag aaaagggggc ttttgtttaa aatgtaagaa actgtcacga aattaaaatg
     9481 aagttcttac ataagcgaat cataaaaaat ttcattttga ttttaaaaca gttcaagaaa
     9541 gtcaaaaaat tattctattt gaaagagagg tgccgactgt gaaagtcaat aaaatccgta
     9601 tgcgggaaac agtgatttcc tacgctttcc tagcaccagt attattcttc tttgtcatct
     9661 ttgtgttggc tccgatggtg atgggcttca ttacaagttt ctttaactac tcaatgacta
     9721 aatttgagtt tgtaggcttg gataactata tccgtatgtt taaagatcct gtctttacaa
     9781 aatctctgat taacacagtt attttggtta ttggatctgt accagttgtt gttctattct
     9841 cactctttgt agcatctcag acctatcatc aaaatgtcat tgccagatcc ttctaccgtt
     9901 tcgtcttctt ccttcctgtt gtaacgggta gtgttgccgt gacagttgtt tggaaatgga
     9961 tttatgaccc gctatcaggg attctaaact ttgtccttaa gtcaagccac atcatcagcc
    10021 aaaacatttc ttggttggga gataaaaact gggcattgat ggcgattatg attattctct
    10081 tgaccacttc agttggtcag cccatcatcc tttatatcgc tgccatgggg aatattgaca
    10141 attcactggt tgaagcggcg cgtgttgatg gtgcaactga gtttcaagtt ttttggaaga
    10201 ttaaatggcc aagccttctt ccaacaactc tttatattgc aatcatcaca acaattaact
    10261 cattccagtg tttcgccttg attcagcttt tgacatctgg tggtccaaac tactcaacaa
    10321 gtaccttgat gtactacctt tacgaaaaag ccttccaatt gacagaatac ggctatgcca
    10381 acacaattgg tgtcttcttg gcagtcatga ttgctatcgt aagctttgtt caatttaaag
    10441 tacttggaaa cgacgtagaa tactaaagaa aggagacagc tatgcaatct acagaaaaaa
    10501 aaccattaac agcctttact gttatttcaa caatcatttt gctcttgttg actgtgctgt
    10561 tcatctttcc attctactgg attttgacag gggcattcaa atcacaacct gatacaattg
    10621 ttattcctcc tcagtggttc cctaaaatgc caaccatgga aaacttccaa caactcatgg
    10681 tgcagaaccc tgccttgcaa tggatgtgga actcagtatt tatctcattg gtaaccatgt
    10741 tcttagtttg tgcaacctca tctctagcag gttatgtatt ggctaaaaaa cgtttctatg
    10801 gtcaacgcat tctatttgct atctttatcg ctgctatggc gcttccaaaa caagttgtcc
    10861 ttgtaccatt ggtacgtatc gtcaacttca tgggaatcca cgatactctc tgggcagtta
    10921 tcttgccttt gattggatgg ccattcggtg tcttcctcat gaaacaattt agtgaaaata
    10981 ttcctacaga gttgcttgaa tcagctaaaa tcgacggttg tggtgagatt cgtaccttct
    11041 ggagtgtagc cttcccgatt gtgaaaccag ggtttgcagc ccttgcaatc tttaccttca
    11101 tcaatacttg gaatgactac ttcatgcaat tggtaatgtt gacttcacgt aacaatttga
    11161 ccatctcact tggggttgcg accatgcagg ctgaaatggc aaccaactat ggtttgatta
    11221 tggcaggagc tgcccttgct gctgttccaa tcgtcacagt cttccttgtc ttccaaaaat
    11281 ccttcacaca gggtattact atgggagcgg tcaaaggata atactctgcg aaaatctctt
    11341 caaactacgt cagcttcacc ttgccatact taagtattgc ctgcggttag cttcctagtt
    11401 tgttcttcaa ttttcattga gtataggaaa atcaatctat caagatacag aagtatattt
    11461 tatagattta gagaatatag aggttataag tgtctacaaa atggagggta tgcagttact
    11521 ttatgaagtt ttgtcagaca cttatcaact taagaatggt tttagttaac tatcagaaac
    11581 gaaggaaaga gtatgatttt tgacgatttg aaaaacatca ccttttacaa agggatttat
    11641 cctaatttag acaaggctat cgactatctc taccaacatc gtaaggattc tttcgaatta
    11701 ggaaagtatg atattgatgg agataaagtc tttctagttg ttcaggaaaa tgtcctcaat
    11761 caagctgaaa atgatcaatt tgagtatcat aagaactatg cagatttgca tttgctgata
    11821 gaagggcatg aatattcgag ctacggttca cgtatcaaag acgaggcagt agcattcgac
    11881 gaagcgagtg acattggctt tgttcattgt tatgaacact acccactctt attgggttat
    11941 cacaattttg cgattttctt cccaggtgag ccacatcagc caaatggtta tgcaggcatg
    12001 gaagaaaagg ttcgaaaata tctctttaaa atcttgattg attaaaaata ggatgaattg
    12061 tttttttgta aagctttgat aatactctac catgaaattg atctttgtga ggtagagaaa
    12121 tgagaataaa atatttaaaa attggtatct tctaagtatg ctgcaagagc tagtttcttg
    12181 gatggacagg gaattacagt tgatgagatg gcttggataa ttaggggcat tgtgaatgca
    12241 ttgattggta gatacataaa attaggtgct tatgcggcta agtatggtat tagtatggca
    12301 cgctcgatct taagtagggc agctgcaact gcggcagcaa gagtaggatt attgaccaag
    12361 atttttggat ggattttacg aatagctgtg ctcgcagctg atgtatatgg taattttgcc
    12421 aacaatattg ctgcagcttg ggatgcatat gataaaattc ctaacaatgg tcgtataaac
    12481 ttttaaaatg cgagaatgaa agcactttgt atttttttat tgaatatgtt agcttggaca
    12541 gtgcttgcaa tgataattcg tggagggcta gatggattta atgagcatac ttggagtact
    12601 attttaattg cttcgctgtc cgggatatat atagattata tgtccataga taaaagtaga
    12661 aaaaagtcca aaagaaaaaa gaaaaaatag atttgttcat ggtagggact tatgaaagtt
    12721 ttactgacaa aaaagaaaac agtttacaaa gaaaaatgat ggaggagcaa acatggcaca
    12781 aaaaggagta agccttatca aggcagcatt tgatacagat aactttctca tgcgttttag
    12841 tgagaaagtc ttggacatcg tgacagccaa tcttcttttt gtcgtctctt gtttgcccat
    12901 cgtgacgatt ggagtggcta aaatcagcct ctacgagacc atgttcgaag ttaagaagag
    12961 cagacgggtg cctgttttta aaatctatct aagatctttc aagcaaaatc tgaaactagg
    13021 tcttcagctg ggtttaatgg agttaggaat tgtgtttctt accctttcag atctctatct
    13081 tttctggggt caaacagctc tgcccttcca attgctgaaa gccatttgtt taggtattct
    13141 gatttttctt actatcgtga tgctggctag ttaccctatc gcggcacgtt atgacctatc
    13201 ttggaaagaa attcttcaaa aaggattgat gttggctagt tttaactttc cttggttctt
    13261 cctcatgtta gccattcttg tcctcattgt gatggttctt tatctgtccg ccttcagtct
    13321 actcttaggt ggctcagtct tcctactttt tgggtttgga ctattggtct ttatccagac
    13381 tggattgatg gagaaaattt tcgcaaaata ccaataggag ctttatttct gaaactactt
    13441 tcaaaggctc caaacgctat tctataagcg agaaactaaa atctaagtgt ctataagata
    13501 ttgaaagcct tttcggtaaa gtgtatacta aagaagtaaa gaaaaatctg ctttagcaga
    13561 aaataaaaat aataggagaa aatctatgtc agatttgaaa aaatacgaag gtgtcattcc
    13621 agccttctac gcatgttatg atgatcaagg agaagtaagc ccagaacgta cgcgtgcctt
    13681 ggttcaatac ttcattgata aaggtgttca aggtctttat gtcaatggtt cttctggtga
    13741 atgtatctac caaagcgttg aagatcgcaa gttgattttg gaagaagtca tggcggtagc
    13801 caaaggtaaa ttgaccatta ttgcccatgt tgcttgcaat aatactaaag atagtatgga
    13861 acttgctcgc catgctgaaa gcttgggagt agatgctatt gcaacgattc caccaattta
    13921 cttccgcttg ccagaatact cagttgctaa atactggaac gatattagtt ctgcagctcc
    13981 aaatacagac tacgtgattt ataacattcc tcaattggca ggggttgctt tgactccaag
    14041 cctttacact gaaatgttga aaaatcctcg tgttatcggt gtgaagaact cttctatgcc
    14101 agttcaagat atccaaacct ttgtgagcct tggtggggaa gaccatatcg tctttaacgg
    14161 tccagatgaa cagttcctag gtggccgcct catgggtgcc aaagctggta tcggtggtac
    14221 ttatggtgct atgccagaac tcttcttgaa actcaatcag ttgattgcgg ataaggacct
    14281 agaaacagcg cgtgaattgc agtatgctat caacgctatc attggtaaat tgactgctac
    14341 acatggaaat atgtacggtg tcatcaaaga agttttgaag atcaatgaag gcttgaatat
    14401 tggttctgtt cgttcaccat tgacaccagt gactgaagaa gatcgtccag ttgtagaagc
    14461 ggctgctgcc ttgattcgtg aaaccaagga gcgcttcctc taatctaaaa ggaggtattt
    14521 atgacatact acgttgcaat tgatatcggt ggaaccaaca tcaagtatgg tttggttgat
    14581 caagaggggc aacttcttga atcgcatgaa atgccaactg aggcgcataa gggtggacct
    14641 catatcttac aaaagaccaa agatatcgta gctagttatt tagaaaaagg cccagtagca
    14701 ggtgttgcca tatcttctgc tgggatggtg gatccggata agggtgagat tttctatgct
    14761 gggccgcaaa tccctaacta cgcaggcacc cagttcaaaa aggaaatcga agaaagcttt
    14821 actattcctt gtgagattga aaatgatgtc aactgtgcag gtcttgctga ggcagtatct
    14881 ggttcaggca agggagcaag tgtgacactt tgcttgacca ttggaacagg tatcggtggt
    14941 tgcttgatta tggataggaa agtcttccat ggttttagca attcagcctg tgaagtcggg
    15001 tatatgcata tgcaggatgg agcttttcaa gacttggctt ctacaacagc tttagtgaaa
    15061 tatgtagctg aagcccatgg agaagatgtt gatcagtgga atggccgtag aattttcaaa
    15121 gaagccactg aaggaaacaa aatctgcatg gaaggtattg accgtatggt tgactatcta
    15181 ggaaaaggtc tggcaaatat ttgctacgtt gccaatccag aagtggttat tcttggtggt
    15241 ggtatcatgg ggcaagaggc tatcctcaaa cctaagatcc gcacagcctt gaaagaggct
    15301 ttggtaccaa gtttagcaga aaaaacacga ttagaatttg cccatcacca aaatacagca
    15361 gggatgttgg gtgcatatta tcattttaag acaaaacaat cctagtttgg ctcagccaaa
    15421 ctaggatttt cttacacgtt tttgtctacg atagccgttg agttttttat tttcccagta
    15481 gctattaaag attttttcct tgctttcgcg attgatttcc aaaaagtagg cataaatcaa
    15541 atcgataaag aagagcatag gaagttgagc ggatattcgt tggatatagg aggtttggct
    15601 atgggtggct acaagaacag tctctgtata ggtctggcta tctttattgg gaacacttgt
    15661 aaagagtaca gtctttgccc ccatctcctt ggcatctaat agactatcta aaatagaagg
    15721 agttgagcct gaaagtgaga aacccagtac tagacaattt tcatccatga tgctggttgt
    15781 ccaggcaaag ccgtcttggt ctgtcaaagc ttcgcagacc acacctagtc gcataaaacg
    15841 taatttcatt tcacgggcga cgaggccaga actccctgtt ccaaagaagt agatacgctc
    15901 agcatcttcg attagctggg caattcgctc tagttggatt tcgtcaatca agtcttgtgt
    15961 ttgttccctc atattgctat aacttctgag gactcgtttg gtcagtggac tatgcttgga
    16021 gacttggttg gcttgatttt ctgcctgatg ttggtattgg aaaataaatt ctcggtagcc
    16081 agtaaagcca cactttttag caaagcgggt caaagcagct tgagaaatat gtaatttttg
    16141 ggtgacttgt tgagaagata aatcatctgt aatcgtttca gcttgcaaaa aatagcgagc
    16201 gatttcttgc tctaggtctg tcatttcttc aaaatgtgaa tcaatgatag ttgcgatatc
    16261 tggtttgtcc atagggaaag ctcctttaca tgagtcatac tggaagacta gatcagggaa
    16321 tagtcacact tcattataac acataatata aggatagata aataaaaaca catctctgtt
    16381 ttaaaaacga aaaaatcgaa aaagcttctt tcttttccat aattttctac tcaaattgtg
    16441 gtacaattaa gagtaagatt ttaagttaga aatgagactg atttgtatga gaaaatttaa
    16501 cagccattcg attccgattc ggcttaattt attgttttca atcgtcattt tactctttat
    16561 gaccattatt ggtcgtttgt tgtatatgca ggttttgaac aaggattttt acgaaaaaaa
    16621 gctagcttca gctagtcaga ccaagattac aagcagttca gcccgtgggg aaatttatga
    16681 tgctagtgga aaacctttgg tagaaaatac gttaaagcag gttgtttcct ttacgcgtag
    16741 caataaaatg acggctacag acttaaaaga aacagctaaa aagttactga cttatgtgag
    16801 catcagttct ccaaatttga cagaacgcca gctggcggat tactatttgg ctgatcctga
    16861 aatctataaa aaaacagtgg aagctctccc aagcgagaaa cgcttgggtt cagatggcaa
    16921 tcgtctatcc gaatcagaac tgtataacaa tgcggtcgat agtgtccaaa caagtcaact
    16981 aaactataca gaggatgaaa agaaagaaat ctatcttttt agtcagttaa atgctgttgg
    17041 aaactttgcg acaggaacca ttgcgacaga tcctctaaat gattctcagg tggctgttat
    17101 tgcctctatt tcaaaggaga tgcctggcat tagtatttct acttcttggg atagaaaggt
    17161 tttggaaact tccctttctt ctatagttgg gagtgtatcc agtgaaaaag ctggtctccc
    17221 agcggaagaa gcagaagcct atcttaaaaa aggctattct ctaaatgatc gtgtaggaac
    17281 ctcctatttg gaaaagcaat atgaagagac cttacaagga aaacgctcgg taaaagaaat
    17341 ccatctggat aaatatggca atatggaaag cgtggataca attgaggaag gtagtaaggg
    17401 aaacaatatc aaactgacca ttgatttggc attccaagat agcgtggatg ctttactgaa
    17461 aagttatttc aattccgagc tagaaaatgg tggagccaag tattctgaag gtgtctatgc
    17521 agtcgccctt aacccaaaaa caggtgcggt tttgtctatg tcagggatta aacatgactt
    17581 gaaaacggga gagttgacgc ctgattcctt gggaacggta accaatgtct ttgttccagg
    17641 ttcggttgtc aaggcggcga ccatcagctc aggttgggaa aatggagtct tgtcaggaaa
    17701 ccagaccttg acagaccagt ccattgtctt ccaaggttca gctcccatca attcttggta
    17761 tactcaggct tacggttcat tccctatcac agcggtccaa gctctggagt attcatctaa
    17821 tacctatatg gtccaaacag ccttaggtct tatggggcaa acctatcaac ccaatatgtt
    17881 tgtcggcacc agcaatctag agtctgctat ggagaaactg cgttcaacct ttggcgaata
    17941 tggcttgggt actgcgacag gaattgacct accagatgaa tctactggat ttgttcccaa
    18001 agagtatagc tttgctaatt acattactaa tgcctttggg cagtttgata actatacgcc
    18061 gatgcagttg gctcagtatg tagcaactat tgcaaataat ggtgttcgtg tggctcctcg
    18121 tattgttgaa ggcatttatg gtaataatga taagggagga ctgggtgact tgattcagca
    18181 actgcaaccg acagagataa ataaggtcaa tatatccgac tccgatatga gcatcttgca
    18241 ccaaggtttt tatcaggttg cccatggtac tagtggattg acaactggac gtgccttttc
    18301 aaatggtgcc ttggtatcca ttagcggaaa aacaggtaca gccgaaagct atgtggcaga
    18361 tggtcagcaa gcaaccaata ccaatgcggt ggcctatgcc ccatctgata atccccaaat
    18421 cgctgtcgca gtggtctttc ctcataatac caatctaaca aatggtgtag gaccttccat
    18481 tgcgcgtgac attatcaatc tgtatcaaaa ataccatcca atgaactaga aaggaaatta
    18541 tgctttatcc aacacctatt gccaagttga ttgacagtta ttctaagtta ccaggtatcg
    18601 ggattaagac ggctacgcgt ctggcctttt atacgattgg gatgtctgct gatgatgtca
    18661 atgaatttgc aaaaaatctc ctttctgcta agagagaatt gacatattgt tctatttgtg
    18721 gacgtttgac agacgacgat ccttgttcta tctgtactga ttcgactcgt gaccagacaa
    18781 caattttagt tcttgaggat agtagagatg tggcagccat ggaaaatatc caagaatacc
    18841 atggactcta tcatgtcctg catggcctca tttctcctat gaatggtatc agtccggacg
    18901 atatcaatct taagagcctt atgactcgtc ttatggatag tgaggtttca gaagtgattg
    18961 tggcgactaa tgctacagcg gatggtgaag cgacctccat gtatctttca cgtttgctca
    19021 agccggctgg tatcaaggtt acgcgtctag cacgaggtct cgctgtggga gcggacattg
    19081 agtatgcgga cgaagtgaca ctcttacgag cgattgaaaa tcggacagag ttgtaagtgt
    19141 aggcaaattt acgaactcca ttcatttata aaaaatcaaa gaggctgaaa atcgttccta
    19201 tcggcctctt tttgtatagt gtgatgagta gaatcaggtt caagttttaa aaaaccaagc
    19261 aaatatgata tactaaagag cgagtattct agtagaatta ggacaaataa tatgaaacaa
    19321 acgattattc ttttatatgg tggacggagt gcggaacgcg aagtctctgt cctttcagct
    19381 gagagtgtca tgcgtgcggt caattacgac cgtttcacag tcaagacttt ctttatcagt
    19441 cagtcaggtg actctatcaa aacgcaggaa tttagtcatg ctccggggca agaagaccgt
    19501 ctcatgacca atgaaaccat tgattgggat aagaaagttg caccaagtgc tatctacgaa
    19561 gaaggtgcag tggtctttcc agtccttcac gggccaatgg gagaagatgg ctctgttcaa
    19621 ggattcttgg aagttttgaa aatgccttac gttggttgca acattttgtc atcaagtctt
    19681 gccatggata aaatcacgac taagcgtgtt ctggaatctg ctggtattgc ccaagttcct
    19741 tatgtggcta tcgttgaagg cgatgatgtg actgctaaaa tagctgaagt ggaagaaaaa
    19801 ttggcttatc cagtcttcac taagccgtca aacatggggt ctagtgtcgg tatttctaag
    19861 tctgaaaacc aagaagaact ccgtcaagcc ttaaaacttg ccttccgata tgacagccgt
    19921 gtcttggttg agcaaggagt gaatgcccgt gaaattgagg ttggcctctt gggtaactac
    19981 gatgtcaaga gcacgctacc tggagaagtt gtcaaggacg ttgcctttta tgactacgat
    20041 gccaagtata ttgataacaa ggttactatg gatattcctg ccaaaatcag tgatgatgtg
    20101 gtggctgtca tgcgtcaaaa tgcagaaaca gccttccgtg ccattggtgg ccttggtcta
    20161 tctcgttgcg atttcttcta tacagataag ggagagattt ttctcaacga gctcaatacc
    20221 atgccaggtt tcacccagtg gtctatgtac ccactacttt gggaaaatat ggggatcagc
    20281 tacccagaac taatcgagcg tttggttgac cttgccaagg aaagttttga caagcgcgaa
    20341 gcgcatttga tataaaatga aagagagggt agaagccaga accatcactg caaggtgact
    20401 agagttctcg gacttcagcc ctttttaaag gagtagaaat gaaattaaca atccatgaaa
    20461 ttgcccaagt tgttggagcc aaaaatgata tcagtatctt tgaggacact cagttagaaa
    20521 aagctgagtt tgatagtcgt ttgattggaa ctggagattt atttgtgcca cttaaaggtg
    20581 cgcgtgatgg ccatgacttt attgaaacag cctttgaaaa tggtgcagca gtaaccttgt
    20641 ctgagaaaga ggtctcaaat catccttaca ttttagtaga tgatgttttg acagcctttc
    20701 aatccctagc atcctactat cttgaaaaaa cgactgttga tgtctttgct gttacaggtt
    20761 caaatggcaa gacaacgact aaggatatgt tggcgcattt actgtcaaca agatacaaga
    20821 cctacaaaac acaaggcaat tacaataatg agattggcct tccttacaca gttcttcata
    20881 tgcctgaagg aacagaaaag ttggttttgg agatgggaca ggatcacttg ggagatattc
    20941 atctcttgtc tgaattggct cgtccaaaaa cagccatcgt gaccttggtt ggagaagctc
    21001 atttggcctt tttcaaagat cgttctgaga ttgctaaagg aaaaatgcaa attgcagacg
    21061 gaatggcttc aggttccttg cttttagcgc cggctgaccc tatcgtagag gactatttgc
    21121 caattgataa aaaggtggtt cgttttggtc aaggagcaga gctggaaatt actgacttgg
    21181 ttgagcgtaa ggacagtctg acctttaagg ctaatttctt ggaacaagcc cttgatttgc
    21241 cagtaactgg caagtacaat gcgacaaatg ctatgattgc atcctatgtt gccttgcaag
    21301 aaggagtttc agaggagcaa attcgtttgg cattccaaca tcttgaattg acgcgtaacc
    21361 gtaccgagtg gaagaaagca gccaatggag cagatatcct gtcagatgtt tacaatgcca
    21421 atccaactgc tatgaaactg attttagaga ctttctctgc cattccagcc aatgaaggtg
    21481 gcaagaaaat tgcagtgttg gcggatatga aggagcttgg tgaccagtct gttcaacttc
    21541 ataatcagat gattttgagc ctctccccag atgtgcttga tatcgttatt ttctatggag
    21601 aagatattgc tcagttggct caattggcca gtcaaatgtt tccaatcggc cacgtttact
    21661 acttcaagaa aacagaagac caggatcaat ttgaagacct agtcaagcag gttaaggaaa
    21721 gccttggagc ctatgaccaa atcttgctaa aaggttctaa ctctatgaat ctagccaaat
    21781 tggtagaaag tttagaaaat gaatgcaagt gattttgcta agtatcttca aagaatgatt
    21841 gctcttacag atactggact aacctttaca aaggatccgt ttgaccgtga gcgctacgaa
    21901 gacttgcgaa gtctgttatc tgaaatgttg aatcaggtat cagacctgga tgcagacgaa
    21961 gtggcagaac tcttgaaacc gacctccgct tatgcgactc ctttaatgga cgtccgtgct
    22021 tggatagttg aggatgaaaa aatttgtctg gttaggggac aaggagagaa tgattgggct
    22081 ttgccaggtg gctttggcga ggtcggttat tcaccaactg aaaatattct caaggaaatt
    22141 gaagaagaaa ccggttttaa agccaaagtt gaacgactac tagctgtttt tgataccaat
    22201 cgtttccaac tacagagcaa acaatatgca aagtttgttt ttgaatgtaa gcttcacgat
    22261 ggacaatttc aagaaaatca agaaatcgct gaccttcaat tttttgcaat tgaccaactg
    22321 ccgaatttat ctgaaaaacg cattaccaag gagcaaatag agcttctttg gcaggtttat
    22381 caaggtcata gggggcaata tcttgactaa gaagatgatt atcgtatttc taaatccatt
    22441 tttaacaact agcatggtat aataatatgc aggaaaattt tgaattatga ggaagactag
    22501 atgaatttat gggatatttt ctttacgact caggcaaccg agccgcccaa atttgacctt
    22561 ttttggtatg ttagcctatt tacgctctta gccttaacct tttatacagc ccatcgctat
    22621 cgtgaaaata aggtttacca acgatttttc caaatcttgc agactgttca gttaatcctt
    22681 ctttatggtt ggtactgggt caatcatatg ccactgtcag aaagcctacc cttttaccat
    22741 tgccgtatgg ctatgtttgt ggtactcttg cttcctggtc aatccaaata taaacaatac
    22801 tttgcattat tgggaacatt tgggacatta gcagcctttg tttatccagt gccagatgct
    22861 tacccttttc cacatatcac cattctatcc tttatctttg gtcatttagc actcttgggg
    22921 aactctctag tttatctatt gagacagtat aatgcgcgat tgctggatgt gaagggaatt
    22981 tttctcatga cctttgccct aaatgccttg atttttgtgg tcaatttggt gacaggtggc
    23041 gattacggat ttttgacaaa accgccattg gttggggatc acggtctagt agctaattat
    23101 ttacttgttt caattgtgct ggtagctact atcagtttga ctaagaaaat cttagaattc
    23161 tttttagctc aagaagcaga aaaaatgatt gcaaaggaag cttaacacag agctttcttt
    23221 tttgctctta gagagttttt acaagcagct tataaaataa gaatttctga atagacaaac
    23281 tcaaaaaatg gctgggaaat ttaggaaaaa agcaagcacg attaaatttt ttgtgttata
    23341 atattttgtg aatagctatg cctatgttta gctatggaat aatacgaagt gcgaaacttg
    23401 gaagatagag aggaagcgat gtaatggcta gagaaggctt ttttacaggt ctagatattg
    23461 gaacaagctc tgtcaaggtg cttgtggccg agcagagaaa tggtgaatta aatgtaattg
    23521 gcgtgagtaa tgccaaaagt aaaggtgtaa aggatggaat tattgttgat attgatgcag
    23581 cagcaactgc tatcaagtca gccatttccc aagcggaaga aaaggcaggc atttcgatta
    23641 aatcagtgaa tgtcggcttg cctggtaatc ttttgcaggt agaaccaact caggggatga
    23701 ttccagtaac atctgatact aaggaaatta cggatcaaga tgttgaaaat gttgtcaaat
    23761 cagctttgac aaagagtatg acacctgacc gtgaagtcat tacctttatt cctgaagaat
    23821 ttattgtgga tggtttccaa gggattcgtg acccacgtgg catgatgggg gttcgccttg
    23881 aaatgcgtgg tttgctttat acaggacctc gtactatctt gcacaatttg cgtaagacgg
    23941 ttgagcgtgc aggtgttcag gttgaaaatg ttatcatttc accactagca atggttcagt
    24001 ctgttttgaa cgaaggggaa cgtgaatttg gtgctacagt gattgatatg ggggcaggtc
    24061 aaacgactgt cgctacaatc cgtaatcaag aactccagtt cacacatatt ctccaagaag
    24121 gtggagatta tgtaactaaa gatatctcca aggttttgaa aacctctcgc aaattagcgg
    24181 aaggcttgaa actgaattac ggggaagcct atccgcctct tgcaagcaaa gaagccttcc
    24241 aagtagaggt tattggagaa gtagaagcag tcgaagtgac ggaagcctac ttgtcagaaa
    24301 ttatttctgc acgaatcaag cacatccttg aacaaatcaa gcaagaatta gatagaaggc
    24361 gtctattgga cctccctggt ggtattgtct taatcggtgg gaatgccatt ttaccaggta
    24421 tggttgagct tgctcaggaa gtctttggcg tccgtgtcaa gctttatgtt ccaaatcaag
    24481 ttggtatccg taatccagcc tttgcgcatg tgattagttt atcagaattt gcgggtcaat
    24541 taacagaagt taatcttttg gctcagggag cgataaaagg tgagaatgac ttaagtcatc
    24601 agccaattag ttttggtggg atgctgcaaa aaacagctca gtttgtacaa tcaacgcctg
    24661 ttcaaccagc tcctgctcca gaagtagagc tggtggcgcc tacagaacca atggcggatt
    24721 tccaacaagc ttcacaaaat aaaccgaaat tagcagatcg tttccgtggc ttgatcggaa
    24781 gcatgtttga cgaataaaga ggaaaaataa attatgacat tttcatttga tacagctgct
    24841 gctcaagggg cagtgattaa agtaattggt gtcggtggag gtggtggcaa tgccatcaac
    24901 cgtatggtcg acgaaggtgt tacaggcgta gaatttatcg cagcaaacac agatgtacaa
    24961 gcattgagta gtacaaaagc tgagactgtt attcagttgg gacctaaatt gactcgtggt
    25021 ttgggtgcag gaggtcaacc tgaggttggt cgtaaagccg ctgaagaaag cgaagaaaca
    25081 ctgacggaag ctattagtgg tgccgatatg gtcttcatca ctgctggtat gggaggaggc
    25141 tctggaactg gagctgctcc tgttattgct cgtatcgcca aagatttagg tgcgcttaca
    25201 gttggtgttg taacacgtcc ctttggtttt gaaggaagta agcgtggaca atttgctgta
    25261 gaaggaatca atcaacttcg tgagcatgta gacactctat tgattatctc aaacaacaat
    25321 ttgcttgaaa ttgttgataa gaagacaccg cttttggagg ctcttagcga agcggataac
    25381 gttcttcgtc aaggtgttca agggattacc gatttgatta ccaatccagg attgattaac
    25441 cttgactttg ccgatgtgaa aacggtaatg gcaaacaaag ggaatgctct tatgggtatt
    25501 ggtatcggta gtggagaaga acgtgtggta gaagcggcac gtaaggcaat ctattcacca
    25561 cttcttgaaa caactattga cggtgctgag gatgttatcg tcaacgttac tggtggtctt
    25621 gacttaacct tgattgaggc agaagaggct tcacaaattg tgaaccaggc agcaggtcaa
    25681 ggagtgaaca tctggctcgg tacttcaatt gatgaaagta tgcgtgatga aattcgtgta
    25741 acagttgtcg caacgggtgt tcgtcaagac cgcgtagaaa aggttgtggc tccacaagct
    25801 agatctgcta ctaactaccg tgagacagtg aaaccagctc attcacatgg ctttgatcgt
    25861 cattttgata tggcagaaac agctgaattg ccaaaacaaa atccacgtcg tttggaacca
    25921 actcaggcat ctgcttttgg tgattgggat cttcgccgtg aatcgattgt tcgtacaaca
    25981 gattcagtcg tttctccagt cgagcgcttt gaagccccaa tttcacaaga tgaagatgaa
    26041 ttggatacac ctccattttt caaaaatcgt taagtaaatg aatgtaaaag aaaatacaga
    26101 acttgttttt cgagaagttg cagaggctag tctgagtgct aatcgagagc gtggttcggt
    26161 ctctgtcatt gcagttacca agtatgtaga tgtaccgaca gcggaagcct tgcttccgct
    26221 aggtgttcat catatcggtg aaaatcgtgt agataagttt ctggaaaaat atgaagcttt
    26281 aaaagatcga gatgtgactt ggcatttgat tggtaccttg caaagacgta aggtgaaaga
    26341 tgtcattcaa tacgttgatt atttccatgc attggactca gtaaagctag caggggaaat
    26401 tcaaaaaaga agtgaccgag tcatcaagtg tttccttcaa gtaaatattt ctaaagaaga
    26461 aagcaaacac ggtttttcga gagaggaact gctggaaatc ttgccagagt tagccagact
    26521 agataagatt gaatatgttg gtttaatgac gatggcacct tttgaggcta gcagtgagca
    26581 gttgaaagag attttcaagg cgacccaaga tttacaaaga gaaattcaag agaaacaaat
    26641 tccaaatatg cctatgaccg agttaagtat gggaatgagt cgtgattata aagaagcgat
    26701 tcaattcggt tccacttttg ttcgtatagg tacatcattt tttaagtagg agagaaccat
    26761 gtctttaaaa gatagattcg atagatttat agattatttt acggaggatg aggattcaag
    26821 tctcccttat gaaaaaagag atgagcctgt gtttactcca gtaaattctt cacaggaacc
    26881 ggctctccca atgaatcaac cttcacagtc ggctggtaca aaagagaaca atatcaccag
    26941 acttcatgca agacaacagg aattggcaaa tcagagtcag cgtgcaacgg ataaggtcat
    27001 tatagatgtt cgttatccta gaaaatatga ggatgcaaca gaaattgttg atttattggc
    27061 aggaaacgaa agtatcttga ttgactttca gtatatgaca gaggtgcagg ctcgtcgttg
    27121 tttggactat ttggatggag cttgtcatgt tttagctgga aatttgaaaa aggtagcttc
    27181 taccatgtat ttgttgacac cagtgaacgt tattgtaaat gttgaagata tccgtttacc
    27241 agatgaagat caacagggtg agttcggttt tgatatgaag cgaaatagag tacgataatg
    27301 atttttttaa ttcgtatgat ttataatgca gtggatattt actccctgat tttggtagcc
    27361 ttcgctgtca tgtcttggtt tccaggtgcc tacgaatcca gtttaggtcg ttggattgta
    27421 gcgttggtga aaccagtgct tgctcccttg caacgcctgc ctttacagat agcgggtctt
    27481 gatttatctg tttgggttgc gattgttttg gttcgatttt taggagaaaa cctagtgcgt
    27541 tttctggcga tgataggatg aataaaggga tttatcagca tttctccata gaagatcgtc
    27601 catttcttga caagggaatg gaatggataa agaaggtaga agatagctat gctccttttt
    27661 taactccttt tatcaatcct catcaggaga agctattaaa gattttggcc aaaacctatg
    27721 gtcttgcttg tagcagtagt ggggaattcg tctcgagtga gtatgttcga gttttattat
    27781 acccagatta tttccaacca gagttttcag attttgaaat atctctccag gaaattgtgt
    27841 attccaataa atttgaacat ttaacgcatg ctaagatttt agggacagtc atcaatcaat
    27901 tagggattga acggaaactt tttggagata tcctagtaga tgaagaacgg gcgcagatta
    27961 tgattaatca gcagtttctt cttctctttc aagatggact aaagaaaatt ggtcgtatac
    28021 ctgtttcgct ggaggaacgt cctttcaccg agaaaataga taagctagaa cagtatcgag
    28081 aactggattt atctgtgtct agttttcgat tagatgttct tttatcaaat gttttgaaac
    28141 tatctaggaa tcaagcaaac cagttgattg aaaagaaact tgtccaagta aattatcatg
    28201 tggtagacaa atcagattac actgttcaag ttggagactt gattagtgtg agaaaatttg
    28261 gtcgcttgag attacttcaa gataagggac aaacgaaaaa agagaagaaa aaaataaccg
    28321 tccagttatt attaagtaag tgaggaatag aatgccaatt acatcattag aaataaagga
    28381 caagactttt ggaactcgat tcagaggttt tgatccagaa gaagtcgatg aatttttaga
    28441 tattgtggtt cgtgattacg aagatcttgt gcgtgcgaat catgataaaa atttgcgtat
    28501 taagagttta gaagagcgtt tgtcttactt tgatgaaata aaagattcat tgagccagtc
    28561 tgtattgatt gcccaggata cagctgagcg agtgaaacag gcggcgcatg aacgttcaaa
    28621 caatatcatt catcaagcag agcaagatgc gcaacgcttg ttggaagaag ctaaatataa
    28681 ggcaaacgag attcttcgtc aagcaactga taatgctaag aaagtcgctg ttgaaacaga
    28741 agaattgaag aacaagagcc gtgtcttcca ccaacgtctc aaatctacaa ttgagagtca
    28801 gttggctatt gttgaatctt cagattggga agatattctc cgtccaacag ctacttatct
    28861 tcaaaccagt gatgaagcct ttaaagaagt ggttagcgaa gtacttggag aaccgattcc
    28921 agctccaatt gaagaagaac caattgatat gacacgtcag ttctctcaag cagaaatgga
    28981 agaattacaa gctcgtattg aggtagccga taaagaattg tctgaatttg aagctcagat
    29041 taaacaggaa gtggaaactc caactcctgt agtgagtcct caagttgaag aagagcctct
    29101 gctcgtccag ttggcccaat gtatgaagaa ccagaagtag ctccagtgca tccgacaggt
    29161 ccaacaccag ctacagaaac tgttgattca gcaccgggat ttgaagcacc gcaagaatct
    29221 gttacaattt tataagaaat attctgagaa caatatctta tccttatatt cccagcgagc
    29281 aggagatggt gtgagtcctg cattccctat cgataagatt atcctctcaa actatcaagt
    29341 ctgaatctag taagatttga cgttccccac gttacgggat aagagggaga aagactaaaa
    29401 ctttttccga ataaaggtgg taccacgatt ttcgtccttt ttggaagtcg tggtttttaa
    29461 tttgttatta tttataaagg agataccatg aaactaaaag atacccttaa tcttgggaaa
    29521 acagctttcc caatgcgtgc aggccttccg accaaagagc cagtttggca aaaggaatgg
    29581 gaagatgcaa aactttacca acgtcgtcaa gaactaaatg aagggaaacc gcattttgtc
    29641 ttgcatgatg gccctccata cgcaaatgga aatatccacg ttggacatgc catgaatcat
    29701 atctcaaaag atatcatcat tcgttctaag tccatgtcag gttttaactc accttatatc
    29761 ccaggttggg atactcatgg tctgccaatc gagcaagttt tggcaaagca aggtgttaag
    29821 cgcaaagaaa tggatttggt tgagtacttg aaactttgcc gtgaatacgc tctttctcaa
    29881 gtttataaac aacgtgatga tttcaagcgc ttggggatgt caggtgactg ggaaaattta
    29941 tatgtgactt taactccaga ctacgaagca gcccaaatcc gtgtatttgg tgagatggct
    30001 aataagggtt atatctaccg tggtgctaag ccagtttact ggtcttggtc atctgagtca
    30061 gcccttgccg aagcggaaat tgaataccat gatttggttt caacttccct ttactatgcc
    30121 aataaggtaa aagatggcaa aggtgttcta gatacagata cttatatcgt tgtttggaca
    30181 actactccat ttactatcac agcttctcgt ggtttgactg ttggtgcaga tattgattac
    30241 gttttggttc aacctgctgg tgaaactcgt aagtttgtcg ttgctgcaga attgttgact
    30301 agcttgtctg agaaatttgg ctgggttgat gttcaagttt tggcaactta ccgtggtcaa
    30361 gaactcaacc acatcgtaac agaacatcca tgggatacag ctgtagatga attggttatc
    30421 cttggtgacc acgttacaac tgactctggt acaggtattg tccatacagc ccctggtttt
    30481 ggtgaggacg attacaatgt tggtattgct aataatcttg aagtcgcagt gactgttgat
    30541 gaacgtggta tcatgatgaa gaatgctggt cctgaatttg aaggtcaatt ctatgaaaag
    30601 gtagttccaa ctgttattga aaaacttggt aacctccttc ttgtccaaga agaaatctct
    30661 cactcatatc catttgactg gcgtaccaag aaaccaatta tctggcgtgc agtaccacag
    30721 tggttcgcct ctgtttcaaa attccgccaa gaaatcttgg atgctattga taaagttaaa
    30781 ttccatacag aatggggtaa agtacgtcta tataatatga ttcgtgaccg tggtgactgg
    30841 gttatctctc gtcaacgtgc ttggggtgtt ccacttccaa tcttctatgc agaagacggt
    30901 acagctatca tgacagctga gactattgaa catgtggcgc aactctttga agtacatggt
    30961 tcaagcattt ggtgggaacg tgatgccaag gacctcttgc cagaaggatt tacacatcca
    31021 ggttctccaa atggggagtt caagaaagaa acagatatta tggatgtatg gtttgactca
    31081 ggttcatcat ggaatggtgt tttggtaaat cgtccaaatc tgacttatcc agctgacctt
    31141 taccttgaag gttctgacca ataccgtggt tggttcaact catcactcat tacatctgtt
    31201 gccaaccatg gcgtagcacc ttacaaacaa atcttgtcac aaggttttgc ccttgatggt
    31261 aaaggtgaga agatgtctaa atctcttgga aataccattg ctccaagcga tgttgaaaaa
    31321 caattcggtg ctgaaatctt gcgtctctgg gtaacaagtg ttgactcaag caatgacgtg
    31381 cgtatctcta tggatatttt gagccaagtg tctgaaactt accgtaagat tcgtaacact
    31441 cttcgtttct tgattgccaa tacatctgac tttaacccag ctcaagatgt agtcgcttac
    31501 gataaacttc gttcagttga taagtacatg actattcgct ttaaccagct tgtcaagacc
    31561 attcgtgatg cctatgcgga ctttgaattc ttgacgatct acaaggcctt ggtgaacttt
    31621 atcaacgttg acttgtcagc cttctacctt gattttgcca aagatgttgt ttacattgaa
    31681 ggtgccaaat cactggaacg ccgtcaaatg cagactgtct tctatgacat tcttgtcaaa
    31741 atcaccaaac tcttgacacc aatccttcct cacactgcgg aagaaatctg gtcatatctt
    31801 gagtttgaaa cagaagactt cgtccaattg tcagaattac cagaagttca aacttttgct
    31861 aaccaagaag aaatcttgga tacatgggca gccttcatgg actttcgtgg acaagcacaa
    31921 aaagccttgg aagaagctcg taatgcaaaa gttatcggta aatcacttga agcacacttg
    31981 acagtttatc caaatgaagt tgtgaaaact ctactcgaag cagtaaacag caatgtagca
    32041 caacttttga tcgtgtctga gttgaccatc gcagaaggac cagctccaga agctgccctt
    32101 agcttcgaag atgtagcctt cacagttgaa cgtgctgctg gtgaagtatg tgaccgttgc
    32161 cgtcgtattg acccaacaac agcagaacgc agctaccagg tagttatctg tgaccactgt
    32221 gcaagcatcg tagaagaaaa ctttgtggaa gcagtcgcag aaggatttga agagaaataa
    32281 gattgaaaag tctaggcaaa attcaatttg agaagaaaag acaactaatt ttatagtcta
    32341 ttaaacgcat tgtatcacgt ttttgaacac ctgatacgat gcgtttttta tttattttaa
    32401 aaatttgcga ggtatgactt tttatactca acaagaatca aagagaaact tagcaagcta
    32461 acagtagtaa gataaaatag gaatttgata
//
LOCUS       DBERDK010000019        32158 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.019, whole genome shotgun sequence.
ACCESSION   DBERDK010000019 DBERDK010000000
VERSION     DBERDK010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 32158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32158
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.019"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            22..95
                     /locus_tag="ACL73L_000842"
     tRNA            22..95
                     /locus_tag="ACL73L_000842"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:56..58,aa:Ile,seq:gat)
     gene            102..189
                     /locus_tag="ACL73L_000843"
     tRNA            102..189
                     /locus_tag="ACL73L_000843"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:136..138,aa:Ser,seq:gct)
     gene            complement(315..1250)
                     /gene="birA"
                     /locus_tag="ACL73L_000844"
     CDS             complement(315..1250)
                     /gene="birA"
                     /locus_tag="ACL73L_000844"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HGS0771848.1"
                     /translation="MKSYQAVYQILSKETDYISGEKIAEKLSLSRTAIWKAIKRLEQE
                     GIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKE
                     KAASLFKATAPITRNELIIEIWRAFFETPAEELLYLYKKQSFILGKEVTFTLEQKDYK
                     GLAKDISENGKLLVQCDNGKEIWLNSGEISLNSWK"
     gene            complement(1247..2107)
                     /locus_tag="ACL73L_000845"
     CDS             complement(1247..2107)
                     /locus_tag="ACL73L_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HGS0771849.1"
                     /translation="MLVFSEYQTGTIDLALSFYGYEECTPNYSFCPAIRDTYVLHYIT
                     KGQGKFHYKGKIVDLKEGDFFLLKPEELTFYQADSKEPWAYYWLGITGGKAPDYFALS
                     QISDQSYLIQSETCHTQTTAKLISDIVRFAQITKSSELAQLHIMGQLHELMFHLGTIA
                     PNQKKKNISSTHQLYLECKRLIDSHYPQSLTIQDLAKELSVHRSYLSSVFKEFNTLSP
                     KEYLLYVRMHRARQLLENTQESIKVIAYSVGFSDPLHFSKAYKQYFNQTPSHTRKEYS
                     QYQLVRKATL"
     gene            2215..4377
                     /locus_tag="ACL73L_000846"
     CDS             2215..4377
                     /locus_tag="ACL73L_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HGS0771850.1"
                     /translation="MGVRIENNLFYVESKNLSLIIENRNGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHSVTEVTDFRFVE
                     AKILKGQNGPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTTFNNDATIASYSKLD
                     NNSNQEVVIHKDFSFMADFPAADYEIVTLQGAYAREKTVRRQQVEQGIFSISSNRGAS
                     GHAQTPALLLCEQGVTEDAGNVFAIQLMYSGNFEAFVQKNQLNEVRVAIGINPENFSW
                     KLAPEEYFETPVALVTHSDQGLTGISHESQNFVLKHIMLSEFSKKERPILINNWEATY
                     FDFQREKLLELADEAKKVGIELFVLDDGWFGNRFDDNRALGDWVVNEEKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRQHPDWAIQVPDYEHTYSRNQLVLNLANPQVV
                     EYLKSVLDQLLSYHEIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMIRYFPQVWASDNTDAIARLPIQYGSSYLYPTISMGA
                     HVSAVPNHQMGRMTPLETRGHVAMMGNLGYELDLTNLSDEEKATIANQVNLYKELRPV
                     VQLGQQYRLINPDTASNEAAVQFNYGNQTIVTYVRVLSVVETMETTLKLKDLDEEGLY
                     KLQENGEVYSGAELMYAGLTVILSQGDFLSRQYIFRKL"
     gene            4469..5728
                     /locus_tag="ACL73L_000847"
     CDS             4469..5728
                     /locus_tag="ACL73L_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HGS0771851.1"
                     /translation="MEWYKKIGLLATTGLALFGLGACSNYGKSADGTVTIEYFNQKKE
                     MTKTLEEITRDFEKENPKIKVKVVNVPNAGEVLKTRVLAGDVPDVVNIYPQSIELQEW
                     AKAGVFEDLSNKDYLKRVKNGYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKV
                     PETWDEFEQLVKDIVAKGQTPFGIAGADAWTLNGYNQLAFATATGGGKEANQYLRYSQ
                     PNAIKLSDPIMKDDIKVMDILRINGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAI
                     TAINEQKPNFKIGTFMIPGKEKGQSLTVGAGDLAWSISATTKHPKEANAFVEYMTRPE
                     VMQKYYDVDGSPTAIEGVKQAGEDSPLAGMTEYAFTDRHLVWLQQYWTSEADFHTLTM
                     NYVLTGDKQGMVNDLNAFFNPMKADVD"
     gene            5742..6608
                     /locus_tag="ACL73L_000848"
     CDS             5742..6608
                     /locus_tag="ACL73L_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGS0771852.1"
                     /translation="MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLT
                     YNYKFVGLNNFKLLFMDPKFMNAIGFTAIITIAMVVGEIALGIFIARVLNSKIKGQTF
                     FRAWFFFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFLQTSLLGTKWGAIFAAAFV
                     LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSVSMVFILA
                     LKGGLTAFDQVFAMTGGGPNNATTSLGLLVYNYAFKNNQFGYANAIAVILFLLIVVIS
                     IIQLRVSKKFEI"
     gene            6622..7455
                     /locus_tag="ACL73L_000849"
     CDS             6622..7455
                     /locus_tag="ACL73L_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGS0771853.1"
                     /translation="MKQDERKALIGKYILLILGSVLILVPLLATLFSSFKPTKDIVDN
                     FFGFPTNFTWDNFSRLLADGIGGYYWNSVVITVLSLLAIMIFIPMAAYSIARNMSKRK
                     AFTIMYTLLILGIFVPFQVIMIPITVMMSKLGLANTFGLILLYLTYAIPQTLFLYVGY
                     IKILIPESLDEAAEIDGANQFTTYFRIIFPMMKPMHATTMIINALWFWNDFMLPLLVL
                     NRDSKMWTLPLFQYNYAGQYFNDYGPSFASYVVGIISITIVYLFFQRHIISGMSNGAV
                     K"
     gene            7766..9208
                     /gene="gtfA"
                     /locus_tag="ACL73L_000850"
     CDS             7766..9208
                     /gene="gtfA"
                     /locus_tag="ACL73L_000850"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HGS0771854.1"
                     /translation="MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFF
                     PSTGDRGFAPVDYDEVDSAFGDWEDVKRLGERYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKDLFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLD
                     KVRDIAAEYGTELLPEIHEHYSIQFKIADHDYYVYDFALPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYALNEFYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGR
                     NINRHYYSNEEIAKEVQRPVVKALLNLFSFRNRSEAFDLEGTTEIETPTAHSIVIKRQ
                     NKDKSVTAVAEIDLQNQTYRVIENGVEVTF"
     gene            9346..10539
                     /locus_tag="ACL73L_000851"
     CDS             9346..10539
                     /locus_tag="ACL73L_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HGS0771855.1"
                     /translation="MKSIGFIEKLKGLSSKELILLGIILSIFLPFYLFVVVLCLYIIS
                     LIFTGDMKSILQKMGEHPMLLLFLSYSTVISILAQNWMGLVASVGMFLFTIFFLHYQS
                     ILSHKFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFF
                     NPNYYGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAG
                     AIIYLFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMAL
                     FKQNPFWGEGPLTYMHSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRL
                     MMDMSQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLV
                     SDMTD"
     gene            11224..13203
                     /locus_tag="ACL73L_000852"
     CDS             11224..13203
                     /locus_tag="ACL73L_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HGS0771856.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAAKETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KAVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
     gene            13270..14766
                     /locus_tag="ACL73L_000853"
     CDS             13270..14766
                     /locus_tag="ACL73L_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS0771857.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDPAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRTYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGALTFLLALPTIALVY
                     IVRLIGSSIALPDSFPILGAGDWRSYVLPAVILGLLGAPGTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPGAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKGGK"
     gene            14766..15692
                     /gene="oppC"
                     /locus_tag="ACL73L_000854"
     CDS             14766..15692
                     /gene="oppC"
                     /locus_tag="ACL73L_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HGS0771858.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSIRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            15701..16768
                     /locus_tag="ACL73L_000855"
     CDS             15701..16768
                     /locus_tag="ACL73L_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0771859.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIVQGSIDYRGQDLTALSSHKDWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFNEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYDPRHPYTWSLLSSLPQLADDKGDLYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSETHWAKTWLLHEDAPKVEKP
                     AVIANLHDKIREKMGFAHLAD"
     gene            16779..17705
                     /locus_tag="ACL73L_000856"
     CDS             16779..17705
                     /locus_tag="ACL73L_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGS0771860.1"
                     /translation="MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAAELIRRIQMIFQDPAASLNE
                     RATVDYIISEGLYNHRLFKDEEERKEKVQNIIREVGLLAEHLTRYPHEFSGGQRQRIG
                     IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFIS
                     DRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHD
                     YETDKPSMVEIRPGHYVWANQAELARYQKGLN"
     gene            18195..19133
                     /locus_tag="ACL73L_000857"
                     /pseudo
     CDS             18195..19133
                     /locus_tag="ACL73L_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512061.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
     gene            19145..20034
                     /locus_tag="ACL73L_000858"
                     /pseudo
     CDS             19145..20034
                     /locus_tag="ACL73L_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            20047..20850
                     /locus_tag="ACL73L_000859"
     CDS             20047..20850
                     /locus_tag="ACL73L_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0771861.1"
                     /translation="MASLLTLENIHKTFEAGTVNENHVLKGLDLEVEEGDFISVIGGN
                     GAGKSTLMNILAGNLSMDEGDLLLAGKSIKNLSVRKRAKDIARVFQAPKMGTASRLTI
                     EENMAIALRRGQKRGLGWGVKEKDRIQFQEALKELNIGLENRLKVDTQYLSGGQRQAL
                     TLVMAALVKPKLLLLDEHTAALDPKTSQMVMDLTQKIVEHHQLTTLMITHDMNHAIEY
                     GNRLIMLYQGKIVVDVKGEEKKHLTVEDLMHLFQKNSGQSLVSDELVLR"
     gene            complement(20882..21592)
                     /gene="treR"
                     /locus_tag="ACL73L_000860"
     CDS             complement(20882..21592)
                     /gene="treR"
                     /locus_tag="ACL73L_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HGS0771862.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEEGLIKKIRGQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDK
                     IIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIY
                     SYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKDPYVVSIKSKVYLQDGRQFQFTE
                     SRHKLEKFRFVDFAKRKK"
     gene            21777..23744
                     /gene="treP"
                     /locus_tag="ACL73L_000861"
     CDS             21777..23744
                     /gene="treP"
                     /locus_tag="ACL73L_000861"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HGS0771863.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVTAVTHCATRMRFVLGDDKKANVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEAAKSAAKSNQNVV
                     QRVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIAA
                     NWVWNFGYFTVNRIGYQAQVIPALLAGLSLSYLEIFWRKHIPEVISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKWLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGSAVFAYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVTFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFRKAGLFTKTEGDTNLQAEFVAQEEAEFVNHEPVELTSVEII
                     SPLTGQVKELSQATDPIFASGVMGQGLVIEPSQGELTSPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVTVGQQLIRFDMDVIKAAGLVTETPVI
                     ITNQDAYTATIPGTYPTTIQAGASLMVATRI"
     gene            23922..25547
                     /gene="treC"
                     /locus_tag="ACL73L_000862"
     CDS             23922..25547
                     /gene="treC"
                     /locus_tag="ACL73L_000862"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HGS0771864.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTENGIGDFRGIIEKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEEMVCVGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWAPFGDTGKYYLHLFDETQA
                     DLNWRNSNVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     IVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQMLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDTSENAGFSTGTPWLKVGKSYKDINVENEIKGLIFTFY
                     QDLIRLRKEMPIISEGNYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAV
                     YQNGQILISNYEDAEVSEKILLKPYQTLAIYVN"
     gene            25803..26051
                     /locus_tag="ACL73L_000863"
     CDS             25803..26051
                     /locus_tag="ACL73L_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HGS0771865.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            26258..27052
                     /gene="racE"
                     /locus_tag="ACL73L_000864"
     CDS             26258..27052
                     /gene="racE"
                     /locus_tag="ACL73L_000864"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HGS0771866.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASA
                     AIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIHVEHVEL"
     gene            27049..28020
                     /locus_tag="ACL73L_000865"
     CDS             27049..28020
                     /locus_tag="ACL73L_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HGS0771867.1"
                     /translation="MTNKIYEYKDDQDWYVGSYSIFGGVNSLSDYKTDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSTYRLFSFVVDMLNQEMGRNLEVIQRHGALLLVENGQLLYV
                     ELPKEGVNVHDFFETSKVRETLLIATRNEGKTKEFRAIFDKLGYDVENLNDYPDLPEV
                     AETGMTFEENARLKAETISQLTGKMVLADDSGLKVDVLGGLPGVWSARFAGVGATDRE
                     NNAKLLHELAMVFELKDRSAQFHTTLVVASPNKESLVVEADWSGYINFEPKGENGFGY
                     DPLFLVGETGESSAELTLEEKNSQSHRALAVKKLLEVFPSWQSKPSL"
     gene            27996..28517
                     /locus_tag="ACL73L_000866"
     CDS             27996..28517
                     /locus_tag="ACL73L_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HGS0771868.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPD
                     SPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWMEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            28514..28975
                     /gene="cbpB"
                     /locus_tag="ACL73L_000867"
     CDS             28514..28975
                     /gene="cbpB"
                     /locus_tag="ACL73L_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HGS0771869.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVSPDFTI
                     TEVLHKLVDESFLPVVDAEGIFQGIITRKSILKAVNALLHDFSKEYEIRCQ"
     gene            28972..29706
                     /gene="xerD"
                     /locus_tag="ACL73L_000868"
     CDS             28972..29706
                     /gene="xerD"
                     /locus_tag="ACL73L_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HGS0771870.1"
                     /translation="MRDRISAFLEEKQGLSVNSKQSYKYDLEQFLDMVGERISETSLK
                     IYQAQLANLKISAQKRKISACNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEILYL
                     DSFWQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRISKASQQRIVTIP
                     TALLSELEPLMGQTYLFERGEKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFILRQ
                     IENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            29706..30434
                     /locus_tag="ACL73L_000869"
     CDS             29706..30434
                     /locus_tag="ACL73L_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HGS0771871.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLEAKHQERAQYYSKAPTELIYEDAELVHDKTTIDLFLAFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLIGRDQLRLQDLFKEAQNVQEVITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQS"
     gene            30453..31022
                     /gene="scpB"
                     /locus_tag="ACL73L_000870"
     CDS             30453..31022
                     /gene="scpB"
                     /locus_tag="ACL73L_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HGS0771872.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKPQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            31009..31731
                     /locus_tag="ACL73L_000871"
     CDS             31009..31731
                     /locus_tag="ACL73L_000871"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HGS0771873.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVANKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            31731..31973
                     /gene="yidD"
                     /locus_tag="ACL73L_000872"
     CDS             31731..31973
                     /gene="yidD"
                     /locus_tag="ACL73L_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HGS0771874.1"
                     /translation="MKRILIALVRFYQRFISPVFPPSCRFELTCSNYMIQAIEKHGFK
                     GVLMGLARILRCHPWSKTGKDPIPDHFSLKRNQEGE"
BASE COUNT         9308 a         5964 c         7120 g         9766 t
ORIGIN      
        1 aattaccaag ttttttaact tgggcgcgta gctcaggtgg ttagagcgca cgcctgataa
       61 gcgtgaggtc ggtggttcga gtccactcgt gcccattaat tggagaatta ctcaagaggc
      121 tgaagaggac ggtttgctaa atcgttaggt cgggtaactg gcgcaagggt tcgaatccct
      181 tattctccgt tttaatgaga gtttgtaact ctctttttta tttttagagc caataaaact
      241 tcagatccgt aaaacttaat tgaattacta atctgaagtt atttttatat cgtttatatt
      301 ataattgtgt tattttactt ccaactattg agagaaattt cgccactatt tagccagatt
      361 tcttttccgt tatcacattg aactaaaagt tttccatttt ctgagatgtc tttagcaagt
      421 cccttgtagt ctttttgctc tagtgtgaaa gtgacttctt ttcctagaat gaatgactgt
      481 tttttgtata ggtataatag ctcttctgct ggtgtttcga agaaagcacg ccagatttct
      541 atgatcaatt cattccttgt tataggagct gtagctttaa ataagctggc agctttttct
      601 tttaattcct gagggaagtc tttaatagtg aaattgatac ctactccaat aatgatatct
      661 gtgactaagc cagtttctac agaggtcatt gcttcagtaa ggattcctcc aattttatga
      721 ttgtttagat agatatcatt gacccatttt atgtcgacat ctattaaagt taggttctta
      781 atggctttgt agacagctcc agctacaagt agtgtgtagg atggtaattt gtcatagggg
      841 agatttggtt taagatggag tgtcatataa ataccacctt gtggtgagta gaaggaacgt
      901 tgaaaacggc ctcggcctgc tgtttgatag gaagctagat agagggtatt tgcttcatgg
      961 cctaaatcaa ttgcttcttt tgcatctagt tgtgttgatt ttgtttcggg tttaaagctg
     1021 actttaattg gaagattttc ttctagaatc tctggaagaa taaggtcacc attcatcagt
     1081 ttatatcctc tatttttgat actatcaatt tcaatgcctt cttgttctag tcgcttgatg
     1141 gctttccaaa ttgctgttcg gcttagggat agtttttctg cgattttttc tccgctgata
     1201 tagtcggttt ctttagatag gatttggtag acagcttggt aggatttcat aatgttgcct
     1261 ttcttactag ttggtattga gagtattctt ttcttgtatg acttggagtc tgattaaagt
     1321 attgtttata agctttcgaa aaatggagtg gatctgaaaa acctaccgag tatgcaatta
     1381 ccttgatgga ctcttgggta ttttcgagaa gttgtctagc tcggtgcatt cgaacgtaga
     1441 gtaggtattc tttgggtgat aaggtattaa attctttgaa tacgcttgat aagtagcttc
     1501 tgtgaacgga tagttctttt gctaaatctt gaattgtaag tgattgagga tagtggctat
     1561 caattaatcg tttgcattca agatagagtt ggtgggttga tgaaatattc ttttttttct
     1621 gattgggagc aatagttccc agatgaaaca tcagttcatg aagttgtccc atgatatgga
     1681 gttgagctaa ttcacttgat tttgtaatct gagcgaagcg gacaatgtct gagatgagtt
     1741 ttgcagtagt ctgggtatga caagtttcag attggatgag ataggattga tcagaaattt
     1801 gggaaagagc aaaataatca ggggctttcc ctccagtgat tcctaaccag tagtaggccc
     1861 aaggttcttt actatctgct tgataaaagg ttagttcctc tggttttaat agaaagaaat
     1921 ctccttcttt taaatcaaca attttaccct tgtaatgaaa ttttccttgt cctttagtaa
     1981 tgtaatgtag aacgtatgta tcacgaatgg ctggacaaaa agagtaatta ggtgtgcatt
     2041 cctcatatcc ataaaagctt agggcaaggt cgattgttcc agtctggtat tctgaaaaaa
     2101 ctaacatgtg ctacctccta cctaacattt taccatattc ttgagacatt tttctatttt
     2161 ggaaagcgat ttcaggtgat aaaatataat caataaagtg atgaggtgaa gtaaatggga
     2221 gttaggatag agaataatct attttatgtt gagagtaaaa atctaagttt gattattgaa
     2281 aatcgaaatg gctacttact tttgaaacat ttaggaaaga ctattaagaa ctataaaggt
     2341 tccaatagtg tttatgaacg agaccatgcc ttttcaggaa atccaacggc tactaatcga
     2401 acctttagtt tagatactca gcgacagatt tttggacaac atggcttagg agattttagg
     2461 aaaccaacca tacaggttca gcatagtgta actgaagtaa cagactttcg atttgtagaa
     2521 gcaaagattt taaaaggtca gaatggtcca cagggcttac cttctccaca tagcatggac
     2581 gatacagaga ctcttgtctt aatgttagaa gattctaagg ctcaacttag tctgactttg
     2641 tattatacta cttttaataa tgatgcgact attgctagct acagtaaatt agataataat
     2701 agtaatcagg aagttgtcat ccataaagat ttttctttta tggctgattt tccagctgca
     2761 gattacgaaa tagtaactct gcagggtgct tatgctcgtg aaaagactgt tagacgtcaa
     2821 caggtagaac aaggaatctt ttcgattagc tcaaaccgag gtgcttctgg tcatgctcaa
     2881 acaccagctc ttctactatg cgaacaagga gtcacagagg atgctgggaa tgtgtttgct
     2941 attcaactaa tgtatagtgg caactttgaa gcttttgttc aaaaaaatca attgaatgaa
     3001 gttcgggtgg ctattggcat taatccagaa aacttttctt ggaagttagc ccctgaggaa
     3061 tactttgaaa caccggtagc tttagtgact cattcagatc agggattaac tggtattagt
     3121 catgaaagtc agaattttgt actgaagcac attatgctaa gtgaattttc taaaaaagaa
     3181 cgtccaattc taatcaataa ctgggaagct acttactttg actttcagag agaaaaactg
     3241 ttagagttag cagatgaagc taagaaagtt ggcattgaac tttttgtatt agatgatggt
     3301 tggtttggca atcgttttga tgataatcgt gctttaggtg attgggttgt taatgaggaa
     3361 aaactgggtg gaagtctaga aagtctgatt tcagctatcc atgaaagagg tttgcagttt
     3421 ggactttggt tagaacccga aatgatttct gtagatagtg atttgtatcg tcaacatcct
     3481 gactgggcta ttcaggttcc tgattatgag catacttatt ctcggaatca attagtactt
     3541 aatcttgcca atcctcaggt agtagaatac ttgaaaagtg tcttagatca actcctatct
     3601 tatcatgaga ttgattacat taaatgggat atgaaccgca atatcactaa gctagggaat
     3661 ggattaactt atctagagac acagatgcaa tctcatcagt acatgctggg actttacgaa
     3721 ctcgtttctt atctgacaga gaagcacagc catattctct ttgagtcctg ctctggtggt
     3781 ggtggacgaa atgatcttgg tatgatacgc tatttcccac aagtctgggc tagtgataat
     3841 actgatgcca ttgcacgttt accaattcaa tacggttcat cctatctcta tccaaccatt
     3901 tctatggggg ctcatgtgtc agcagtaccg aatcatcaga tgggacgaat gacaccatta
     3961 gaaacacgtg gccatgtagc aatgatggga aatttgggct atgagcttga tttgacaaat
     4021 ttatcagatg aagagaaagc tacgattgct aatcaggtga acttgtataa agaattacga
     4081 ccagtagttc agttaggaca gcagtataga ctaattaatc ctgatactgc atccaatgaa
     4141 gcagctgtac aatttaatta cggaaatcaa acgattgtaa cctacgttcg cgttttatct
     4201 gttgtagaga ccatggaaac aactttaaaa ttaaaagatt tggatgaaga gggactatat
     4261 aaattacagg aaaatggcga agtttactca ggtgcagaac taatgtatgc gggcttaact
     4321 gttatcttat cccaaggaga ttttttgagt agacagtata tttttagaaa actatgatat
     4381 gtactgaccc caaaagttag atttattctg tctaactttt ggggtgcagt tcaataaggg
     4441 tgtataaatt tataaaggag gctttctaat ggaatggtat aaaaaaatcg gacttcttgc
     4501 aactacaggt ttagctttgt ttgggctcgg cgcttgctcc aactatggta aatctgcgga
     4561 tggcacagtg accatcgagt atttcaacca gaaaaaagaa atgaccaaaa ccttggaaga
     4621 aatcactcgt gattttgaga aggaaaaccc taagatcaag gtcaaagtcg tcaatgtacc
     4681 aaatgctggt gaagtattga agacacgcgt tctcgcagga gatgtgcctg atgtggtcaa
     4741 tatttaccca cagtccatcg aactgcaaga atgggcaaaa gcaggtgttt ttgaagattt
     4801 gagcaacaaa gactacctga aacgcgtgaa aaatggctac gctgaaaaat atgctgtaaa
     4861 cgaaaaagtt tacaacgttc cttttacagc taatgcttat ggaatttact acaacaaaga
     4921 taaattcgaa gaactgggct tgaaggttcc tgaaacctgg gatgaatttg aacagttagt
     4981 caaagatatc gttgctaaag gacaaacacc atttggaatt gcaggtgcag atgcttggac
     5041 actcaatggt tacaatcaat tagcctttgc gacagcaaca ggtggaggaa aagaagcaaa
     5101 tcaatacctt cgttattctc aaccaaatgc cattaaattg tcggatccga ttatgaaaga
     5161 tgatatcaag gtcatggaca tccttcgcat caatggctct aagcaaaaga actgggaagg
     5221 tgctggctat accgatgtta tcggagcctt cgcacgtggg gatgtcctca tgacaccaaa
     5281 tgggtcttgg gcgatcacag cgattaatga acaaaaaccg aactttaaga ttgggacctt
     5341 catgattcca ggaaaagaaa aaggacaaag cttaaccgtt ggtgcgggag acttggcatg
     5401 gtctatctca gccaccacca aacatccaaa agaagccaat gcctttgtgg aatatatgac
     5461 ccgtccagaa gtcatgcaaa aatactacga tgtggacgga tctccaacag cgatcgaagg
     5521 ggtcaaacaa gcaggagaag attcaccgct tgctggtatg accgaatatg cctttacgga
     5581 tcgtcacttg gtctggttgc aacaatactg gaccagtgaa gcagacttcc ataccttgac
     5641 catgaactat gtcttgaccg gtgataaaca aggcatggtc aatgatttga atgccttctt
     5701 taacccgatg aaagcggatg tggattagga gtagagagac tatgaaaaaa gtattacaaa
     5761 aatattgggc atgggctttt gtggtcatcc ccctcttgtt acaagcaatt ttcttctatg
     5821 tgccgatgtt tcaaggagcc ttttacagtt ttaccaactg gacaggattg acttataact
     5881 acaaatttgt cggcttaaac aactttaagc tcctcttcat ggatccaaaa ttcatgaatg
     5941 cgattggctt taccgcaatc attacgattg ccatggtggt tggtgagatt gcactcggga
     6001 tcttcattgc gcgtgtcttg aactctaaaa tcaaaggcca aaccttcttc cgtgcttggt
     6061 tcttcttccc agctgtttta tctggtttga cagtggcttt gatcttcaag caagtcttca
     6121 actacggtct tccagcgatt gggaatgccc ttcatattga atttctccaa accagtcttt
     6181 tagggactaa gtggggagca atctttgcgg ctgcctttgt ccttctttgg caaggggtgg
     6241 ctatgcccat catcatcttc ctagctggtt tgcaatctat tccaactgag attacagagg
     6301 cagcaaggat tgatggtgcg actagcaagc aagttttctg gaacattgaa ttgccttact
     6361 tgctaccaag tgtctctatg gtctttatcc tagccctaaa aggtgggctg actgcctttg
     6421 accaagtctt tgccatgacc ggtggtggtc caaacaatgc cacaacctca cttgggctct
     6481 tggtttataa ctatgccttt aaaaacaacc aattcggtta tgccaatgcc attgccgtaa
     6541 tcttgttcct cttaattgta gtgatttcga tcatccaatt gagagtatct aagaaatttg
     6601 aaatttaaga ggagaagcat gatgaaacaa gatgaaagaa aagccctgat tggcaaatac
     6661 attctattga ttctaggatc ggttctgatt ttagtgccgc tccttgctac cctctttagt
     6721 tccttcaaac ccactaagga tattgtagat aatttctttg gctttccaac caacttcaca
     6781 tgggacaact ttagccgtct cttagctgat gggattggag gctattattg gaactctgtc
     6841 gtcatcactg tcttgtcttt acttgcaata atgatcttta tccctatggc agcctactcc
     6901 atcgctcgca atatgagtaa aagaaaagcc tttaccatca tgtataccct cttaatcctc
     6961 ggaatcttcg tacctttcca agtcatcatg attccgatta cggttatgat gagtaaactc
     7021 ggtttggcta atacctttgg tttgatcttg ctctacttga cctatgcgat tccacagacc
     7081 ctctttctct atgttggcta tatcaaaatc ttgattccag aaagtctgga tgaagcagca
     7141 gagatcgatg gggctaatca atttacaacc tatttccgca tcatcttccc aatgatgaaa
     7201 ccgatgcatg cgacaaccat gatcatcaat gccctttggt tctggaatga cttcatgttg
     7261 ccactccttg tcttgaaccg ggattccaaa atgtggactc tgcctttgtt ccaatacaac
     7321 tacgcaggcc aatatttcaa cgactacgga ccaagctttg cctcttacgt ggtcggcatt
     7381 atcagtatca ccattgtcta tctcttcttc caacgccata tcatttcagg aatgagcaac
     7441 ggggcagtga agtaatactc aatgaaaatc aaagagcaaa ctaggaagct agccgcaggc
     7501 tgtacttgag tacggtaagg cgacgctgac gtggtttgaa gagattttcg aagagtataa
     7561 gaagagctca tggaatctat agtctttgac acagaggaag agttcagctt gttgataatt
     7621 ctatgtagaa aacaagaaag tagtactttg tttatcacag tctatattag tacgagtttg
     7681 atgataaata gagatttaaa atgaatttta tttatgaagc cagatcatat gagacttggc
     7741 ttcaattaga aaaaggagag taatgatgcc aattcaaaat aaaaccatgt tgattaccta
     7801 ttctgatagc cttggaaata atcttaaaga cttatatgat aatttggaag agcattttgg
     7861 agatgctatt ggaggagttc accttttacc atttttccca tcaacaggtg atcgtggatt
     7921 tgcgccagtt gactacgacg aagtggactc agcttttggt gattgggagg atgttaagcg
     7981 tttaggtgag agatattatc ttatgtttga ttttatgatt aatcatattt ctcgtcaatc
     8041 caagtattat aaggactatc aagaaaaaca tgaagccagt gaatttaaag atctcttttt
     8101 aaactgggat aagttttggc cagaaaaccg tccgacacag tctgatgtag atttaattta
     8161 caagcgtaag gatcgtgcac caaagcaaga gattgtgttt gaagatggtt cagtggaaca
     8221 tttgtggaat acctttggtg aggagcagat tgatcttgat gtgaccaaag aagtaactat
     8281 ggaatttatc cgtaagacca ttcagcactt ggcaagtaat gggtgtgatt tgattcgtct
     8341 agatgccttt gcttatgcag tgaagaaatt ggatactaat gatttctttg tggaaccaga
     8401 tatttgggat ttattggaca aagttcgaga tatcgctgct gagtatggga cagagctttt
     8461 acctgagatt catgaacact attcgattca gtttaaaata gcagaccatg attactatgt
     8521 ttatgatttt gctcttccaa tggtgacact ttatactctt tacagttcca gaacagagcg
     8581 tttggctaag tggttaaaga tgagcccgat gaagcaattt acgacgctag atacccatga
     8641 tgggattgga gtagtagatg tcaaggatat cctgaccgat gaggagattg actatgcttt
     8701 aaatgaattc tataaggttg gagccaatgt caaacgtaag tactctagtg ccgagtataa
     8761 caacttagat atctaccaaa tcaattcaac ctactattca gcgcttggag atgatgatgt
     8821 caagtatttt ctcgctcgtc taattcaagc ttttgcccca ggtattcctc aggtttacta
     8881 tgtgggtcta ttagcaggca agaatgactt gaaattatta gaagaaacta aagaaggtcg
     8941 aaatattaat cgtcattact atagcaacga ggaaatagca aaagaagtgc aacgacctgt
     9001 tgtgaaggcc cttctcaatc tattttcttt ccgtaaccgt tcagaagcct ttgatctaga
     9061 agggactact gagatagaga caccaacagc tcacagcatt gtaatcaaac gtcaaaataa
     9121 agataagtcc gtaacagcag tagcagaaat tgatttgcaa aatcagactt atcgggtaat
     9181 tgagaatgga gttgaagtaa cattttgaag ccttgatatg gtggataaaa tagggttttt
     9241 attttggaaa acgtttcctt tgttttcaaa ttgctaaaaa agtggtacaa tataggatag
     9301 cttactatta tctgaatcag ctgatttgga gagaaaggat tcattttgaa atcaataggc
     9361 tttattgaaa agctgaaggg gttgtctagt aaagagctga ttttattggg aattatcctg
     9421 agtatctttt taccctttta tctttttgta gttgtactct gtttatatat tatcagtttg
     9481 atttttacag gagacatgaa aagtattctt cagaaaatgg gggagcatcc gatgctgctt
     9541 ctttttctta gctatagtac tgttatatcc attcttgcac aaaattggat gggtcttgtg
     9601 gcttcagtag gaatgtttct atttactatt ttctttttgc actatcagtc gattttatcc
     9661 cataaattct ttcgattgat tttgcagttc gtcttgtttg gtagtgtctt gtcagctgct
     9721 tttgccagtt tagaacattt ccaaattgtg aagaaattta actatgcttt tctttcaccc
     9781 aatatgcagg tgtggcatca gaaccgggca gaagtgacct tctttaatcc taattattat
     9841 ggaattattt gttgtttctg tattatgatt gctttctatc tgtttacaac gaccaagttg
     9901 aattggttga aagtattctg tgtgattgca ggctttgtta atctctttgg tttgaacttt
     9961 actcaaaatc gaactgcctt tcctgctatt atcgctggag caattatcta tctctttacg
    10021 actattaaaa actggaaggc tttttggctt agtattgggg tcttcgcgat tggtttgagt
    10081 ttcctctttt ctagtgattt gggagttcga atgggtactt tagactcttc tatggaagaa
    10141 cgcatttcta tctgggatgc tgggatggcc ttgtttaagc aaaatccttt ttggggtgaa
    10201 gggccattga cctatatgca ctcttatcct cggatacatg ctccttatca tgaacatgcc
    10261 cacagtcttt atattgatac gattctgagt tacggaattg tgggtaccat tttattagtt
    10321 ctgtcttctg ttgctcctgt tcgtttgatg atggatatga gtcaggagtc ggggaaacgt
    10381 ccgattatcg gcctttatct atctttcctt acagtggttg ctgtgcacgg aatttttgac
    10441 ttggctctct tctggattca gtcaggcttt attttcttgc tagttatgtg cagtattcca
    10501 ttggagcatc gaatgttggt atcggacatg acggattaag ttttataagt ttgaaatctt
    10561 ctaccttggg taggagattt tttatacgat gggcatgtcg tgcatctgag gtgtaagtcc
    10621 tctgtaggga tccactaccg gtgaatacaa tagcgctccc caagcctgac tatcgtgagg
    10681 tagcggatta aaatggtctg gggatagatc gttttaagtc tgacgcagga aataagaatt
    10741 gtcagagaaa gcaatagtga aatcgtaggc tatgaataga aacgtgatag cgaggagcat
    10801 atagtggaca aggtagcgag aaattttaaa gtataaaaga tagtcttaat ctatatgtgt
    10861 aaatcacgaa agtaattgaa ttcggaataa ggttggtgtg aaaagacata gtaaactgaa
    10921 tcttgaatac tagaatgata cttctacaat attgttataa tttaatttga attccaaaat
    10981 cacattgttc cgtccgtatt tcattttact gtatgtcttc ctaagctcca aaatttctgt
    11041 gtcaaatttc ctattttcac tgtagtcttt gacacttcga acgacaattt gaatcatata
    11101 ttcaaaaaaa atgaaaaatt tgtgccaaca agcctaaata ttctgaaaat ttgtttgatt
    11161 tttaatggat aatgtgatat aatggttgtc aatctataat tcacataaac agaggagtag
    11221 aaaatgaaaa aaaatcgtgt atttgctaca gcaggtcttg ttttattagc agcaggtgta
    11281 cttgcagcat gcagttcttc aaaatcatct gattcatcag cccctaaagc ttatggctat
    11341 gtttatacag cagacccaga aaccttggac tacctgattt caagtaaaaa tagtacaaca
    11401 gtagtgactt caaatgggat tgatggttta ttcactaacg ataattacgg taatcttgct
    11461 cctgcagttg cagaggattg ggaagtctct aaggatggtt tgacctacac ttataagatt
    11521 cgtaaagggg ttaaatggtt tacctctgat ggagaagaat atgcagaggt gacggctaaa
    11581 gatttcgtga acggtttaaa acacgcagca gataaaaaat cagaagctat gtatttagct
    11641 gaaaattcgg ttaaaggctt ggcagattat ctatcaggaa cttcaacaga tttttcaaca
    11701 gttggtgtca aggcggttga tgattatacg ttacaataca ctttgaacca gcctgaaccg
    11761 ttctggaact ctaagttgac ctattctatt ttctggcctc tgaatgaaga attcgaaaca
    11821 tcaaaaggaa gcgattttgc taaaccaaca gatccgacat ccttgcttta taatggtcca
    11881 ttcttgttga aagggttgac tgcaaaatct tctgtagagt ttgtaaaaaa tgagcaatat
    11941 tgggataaag aaaatgtcca cctagatact atcaatctag cttactatga tggatcagat
    12001 caggagtcgc tagagcgtaa cttcactagt ggagcttata gttatgcccg tctttaccct
    12061 accagctcca actattctaa ggttgcagaa gaatacaagg acaatatcta ttacacacaa
    12121 tcaggctctg ggattgctgg tctgggtgtg aatattgatc gccaaagtta caactatact
    12181 tctaaaacta cagattcaga gaaagtagct actaagaagg cattgcttaa caaagatttc
    12241 cgtcaagcct tgaattttgc tcttgatcgc tcagcttact cagctcaaat caatggtaaa
    12301 gatggagcag ctttagcagt tcgtaattta tttgtaaaac cagactttgt ttcagctggt
    12361 gagaagacct ttggtgattt agtcgctgct caacttcctg cttatggtga tgagtggaaa
    12421 ggtgtgaatt tagctgatgg gcaggatggt ttattcaatg ctgacaaggc caaggcagag
    12481 tttgcgaaag ctaagaaagc tttagaagca gacggcgttc agtttcctat tcatctggac
    12541 gttccagtag accaagcatc aaaaaactat atatctcgta ttcagtcctt taaacaatct
    12601 gtagaaacag ttcttggtgt tgaaaatgtc gttgttgata ttcaacaaat gacaagtgat
    12661 gaattcctta atattactta ctatgctgcc aatgcttcat ctgaggattg ggatgtatcg
    12721 ggaggagttt catgggggcc agactatcaa gacccatcta cttacctgga tattttaaaa
    12781 acaactagca gtgaaactac aaaaacatat ttaggatttg ataatccaaa tagcccttca
    12841 gtagttcaag ttggtttgaa agaatacgat aaattagttg atgaagctgc caaagagaca
    12901 agcgacttga atgtccgtta tgaaaaatat gcagcggctc aagcatggtt gacagatagt
    12961 tcactcttta ttcctgctat ggcttcttct ggtgcagcac cagtgctttc acgaattgtt
    13021 ccatttactg gagcttctgc gcaaacaggc tctaaggggt cagatgttta cttcaaatat
    13081 ttgaaattac aagataaagc ggtgactaag gaagagtatg aaaaagctcg tgaaaaatgg
    13141 ttgaaagaaa aagctgaatc aaatgagaaa gctcaaaaag aattggcaag tcatgtgaag
    13201 taaataattt ctaatagaac tttctctcca tgaggagaag gttcttttgg gatttttaaa
    13261 ggaaataata tgaaaaaata tatttttatg cgtgttttgc ggtcattggt ttcgattttc
    13321 ttagtaacga ctttgaccta cacgattatc tataccttgg ttcctcgaaa attgattttc
    13381 aagcaggatc ctaactataa taaaattgcg acaacggctg ataaacgtga taactatgaa
    13441 aatactgtgt ttgagcgtat gggctacatt gagtattacg atactaaaga gttgcaagaa
    13501 aaggcaagta gcatggattc ttctgtaaca gtagaagcaa atgcgaccaa taaagctatt
    13561 tatgaaaagt acatcaatca attaggtcat ggttggactt tgggagaatt tactgaaagt
    13621 ggtcaattct atgctactcg tgaaattcca atttttgaac gtgtttttca cttctatgct
    13681 aacttgattg acattgacca tacaaataaa atccaagacc ctgaaaatcc agacttgaaa
    13741 cgctaccttc gttttgaaaa tgatccagct atcggatggt cattggtcgg ttcaggaact
    13801 aaacataaat atctcttgta ctttaacagt cagttcccat ttgttcatca aaactttgtg
    13861 aacttgaatt taggtgactc ttacccaacc tatgctaata caccagttct tcaggttatt
    13921 actcaaggtc aaggacaaac caaaactgcc caagttcagt tcccaacagg taagaaaacg
    13981 tcttctgtaa atatttactc aagaacctac aagtcaccta gtcaggctga ctctcgtgaa
    14041 gtagctagct atgggaaaga tgatccttat acagcgactg aaagtaatta ccaatatcca
    14101 tctatgattg tcagctctgc tattactggt ttgattggtt tggttcttgc ctatgctctt
    14161 gccgtgccac ttggttcagc catggctcgt ttcaagaaca cttggattga tagcctctca
    14221 acaggggctt tgaccttctt gcttgctctt ccaacgattg ccttggttta catcgttcga
    14281 ttgattggat catctattgc ccttccagat tcattcccta tcttgggagc tggagattgg
    14341 cgttcttacg ttttaccagc agtcatcctt ggtttgttgg gtgctcctgg tacagccatt
    14401 tggattcgtc gttacatgat tgacttgcaa tctcaagact ttgttcgttt cgctcgtgca
    14461 aaaggtttgt ctgaaaaaga aatttcaaac aaacacatct ttaaaaatgc catggttccg
    14521 ctggtttcag gaattcctgg tgccatcatt ggggttatcg gtggtgcaac ccttactgaa
    14581 acagtcttcg ccttcccagg tatgggtaaa atgttgattg actctgtaaa agcatctaat
    14641 aactctatgg tcgttggtct tgtcttcatc tttacatgta tttctatctt ctcacgtctt
    14701 ttgggagata tttggatgac tattattgac ccacgtatta aattgactga gaaaggaggc
    14761 aaataatgtc tacaatcgat aaagaaaaat ttcagtttgt aaaacgtgac gattttgcct
    14821 ctgaaactat tgatgcgcca gcatattctt actggaaatc agtgtttaaa caatttatga
    14881 agaaaaaatc aactgtagtc atgttgggaa tcttggtagc catcattttg ataagtttca
    14941 tctacccaat gttttctaag tttgatttca atgatgtcag caaggtaaac gactttagta
    15001 ttcgttatat caagccaaat gcggagcatt ggttcggtac tgacagtaac ggtaaatcgc
    15061 tctttgacgg tgtctggttc ggagctcgta actccatcct catttctgtg attgcgacag
    15121 tgattaactt ggttatcggt gtttttgtcg gtggtatttg gggtatttca aaatcagttg
    15181 accgtgtcat gatggaagtt tacaacgtca tctcaaacat cccacctctt ttgattgtta
    15241 ttgtcttgac ttactcaatc ggagctggat tctggaatct gatttttgcc atgagcgtaa
    15301 caacatggat tggtatcgcc ttcatgatcc gtgtgcaaat cttgcgctat cgtgacttgg
    15361 aatacaactt ggcgtcacgt actttgggaa caccaacctt gaagattgtt gccaaaaata
    15421 tcatgcctca attggtatct gttattgtga caaccatgac tcaaatgctt ccaagcttta
    15481 tctcatacga agccttcttg tctttcttcg gtcttggatt accgattaca gtgccaagtt
    15541 tgggtcgttt gatttcggat tattcacaaa acgtaacaac caatgcttac ttgttctgga
    15601 ttccattgac aacccttgtc ttggtatcct tgtccctttt cgtagttggt caaaacttag
    15661 cggatgctag tgatccacgt acacatagat aggagtagaa atgacaaaag aaaaaaatgt
    15721 aattttgact gctcgcgata ttgtcgtgga atttgacgtt cgtgacaaag tattgacagc
    15781 cattcgcggc gtttcccttg aactagttga aggagaagta ttagccttgg taggtgagtc
    15841 aggatcaggt aaatctgttt tgacaaagac cttcacaggt atgctcgaag aaaatggtcg
    15901 cattgtccaa ggtagtattg actaccgtgg tcaggatttg acagctttat cttctcacaa
    15961 ggattgggaa caaattcgtg gtgctaagat tgcgactatc ttccaggacc caatgactag
    16021 tttggacccc attaaaacaa ttggtagtca gattacagaa gttattgtaa aacaccaagg
    16081 aaaaacagct aaagaagcga aagaattggc cattgactac atgaataagg ttggcattcc
    16141 agacgcagat agacgtttta atgaataccc attccaatat tctggaggaa tgcgtcaacg
    16201 tatcgttatt gctattgccc ttgcctgccg acctgatgtc ttgatctgtg atgagccaac
    16261 aactgccttg gatgtaacta ttcaagctca gattattgat ttgctaaaat ctttacaaaa
    16321 cgagtatcat ttcacaacaa tctttattac ccacgacctt ggtgtggtgg caagtattgc
    16381 ggataaggta gcggttatgt atgcaggaga aatcgttgag tatggaacgg ttgaggaagt
    16441 cttctatgac cctcgccatc catatacatg gagtctcttg tctagcttgc ctcagcttgc
    16501 tgatgataaa ggggatcttt actcaatccc aggaacacct ccgtcacttt atactgacct
    16561 gaaaggggat gcttttgcct tgcgttctga ctacgcaatg cagattgact tcgaacaaaa
    16621 agctcctcaa ttctcagtat cagagacaca ttgggctaaa acttggcttc ttcatgagga
    16681 tgctccaaaa gtagaaaaac cagctgtgat tgcaaatctc catgataaga tccgtgaaaa
    16741 aatgggattt gcccatctgg ctgactagga ggaaggaaat gtctgaaaaa ttagtagaaa
    16801 tcaaagattt agaaatttcc ttcggtgaag gaagtaagaa gtttgtcgcg gttaaaaatg
    16861 ctaacttctt tatcaacaag ggagaaactt tctcgcttgt aggtgagtcc ggtagtggga
    16921 aaacaactat tggtcgtgct atcatcggtc taaatgatac aagtaatgga gatatcattt
    16981 ttgatggtca aaagattaat ggtaagaaat cgcgtgaaca agctgcggaa ttgattcgtc
    17041 gaatccagat gattttccaa gaccctgccg caagtttgaa tgaacgtgcg actgttgatt
    17101 atattatttc tgaaggtctt tacaatcacc gtttatttaa ggatgaagaa gaacgtaaag
    17161 agaaagttca aaatattatc cgtgaagtag gtcttcttgc tgagcacttg actcgttacc
    17221 ctcatgaatt ctcaggcggt caacgtcaac gtatcggtat tgcccgtgcc ttggtcatgc
    17281 aaccagactt tgttattgca gatgagccaa tttcagcctt ggacgtttct gtacgtgccc
    17341 aagtcttgaa cttgctcaaa aaattccaaa aagagctcgg cctgacctat ctcttcatcg
    17401 cccatgactt gtcggttgtt cgctttattt cagatcgtat cgcagttatt tacaagggtg
    17461 ttattgtaga ggttgcagaa acagaagaat tgtttaacaa tccaattcac ccatatactc
    17521 aagccttgct ttcagcggta ccaatcccag atccaatctt ggaacgtaag aaggtcttga
    17581 aggtttacga tccaagtcaa cacgactatg agactgataa gccatctatg gtagaaatcc
    17641 gtccaggtca ctatgtttgg gcgaaccaag ccgaattggc acgttatcaa aaaggactaa
    17701 actagtaatg gttttataat ttccatgtca atttttatgg aaattataaa actttttttg
    17761 ttggactgat ttgaaaaact ttgaaagctt cctgaaagaa tttttgaaaa attttaaaaa
    17821 ttatgaaata ttattgacag gtgacgaaaa aggtggtagg ataggaagca tcaaaaaaga
    17881 aagcagaaaa agaaatgaat aagagacaag ccgtaactat gaatcaaaac ttttacttta
    17941 gctactttag atagtgtttg tagacatgtc accatggatg caaacagttc aagcaatttg
    18001 tttgtatcta tgtggctaag atttttgatt gtggtccata tagatgaata tctaaatgga
    18061 ctagctaagt tgtaacttgt taggttattt caagtatccg tttagatagt atctaggcgg
    18121 ttttttgttt tatcttttct gagaaggagt ttcattatga aagttgttcg aaaatcacta
    18181 gccccctctt ggtagtgggg atcctcttga cttctctgat cagtttgcat cagttgaagg
    18241 cagataagaa aagagatgtc tttcgtatcg gtatttcgca gtttattacc caccagtcct
    18301 tagacgctac tagagaagga tttgtggatg agctggccaa acaaggctat gttgaggggg
    18361 aaaatattga gattgatttg caaaatgcac agggagaaca aagaaatcta aaaacgattt
    18421 cccagcaact agcagaatcc agcgatgtcg ttctagccat cgcaaggcct tctgctcaga
    18481 gcttggccaa tacgacgcaa acgacaccag ttatcttttc agctgtaaca gacccagtca
    18541 gtgccaagtt ggttgagtca agagaacatc ctgggggcaa tgtaactggg acaagcgatc
    18601 agtcatcaga tgccatttca acccaaatca atttgataaa gaaagtgttg ctaaaggcta
    18661 aaacgattgg aatcctctat actcagagcg agcccaattc ggttgtttaa aaggatgaag
    18721 ctaagcgact tctgaaagaa aaaggcttta ccgttgttga aaaaacaatc ttggacagta
    18781 ataacatcaa ggcggcagca gagagtttga tggcagaggt ggatatggtt tttgtcccaa
    18841 ctgataatat catttcgtca accatggaaa cagttaagca ggtttctatt aaacacaagg
    18901 ttccagtatt cggtggttca acagaaatga ttgctgttgg tgacttgtat aactacggaa
    18961 ccaattatga agaattggga agacagacag ctcgcatgct gattcgtgtt ttaaaaggtg
    19021 agaaaccaga aaatatagca gttgagttgc ctgaaaaact ggaattgcat actaatcaag
    19081 aaatggcaga tgcattggga attgatatta gtaagttaga aggcaaggaa taaggaggat
    19141 taagatgaat tttgtgttat ctagtttatc agaaggtttg ttgtggtcga ttgtggcgat
    19201 tggggtctac ttgactttcc gtattttgga tattgcggat atgactgctg agggagcctt
    19261 tcctctgggg gcagcagtcg ttgtatcaca gatacaggca ggggcaaatc cttggcttgc
    19321 gattttactt gctttgctgg caggtatggt agcaggtctt gtatcaggaa tgctccacac
    19381 caagatgaaa atcccagctc tcttgacagg gattgtgacc ttgacagggc tctattcaat
    19441 caatattaaa atcatgggca gtgtactcaa tctttccttg ggagattctg caaccgtctt
    19501 taaacagttg gcgagcttgg gtctgacaaa tgaaggagct gttttctcac ttagtttagt
    19561 ctgtctctta cttgtttggt cttgaccctt ttgatgaaaa cagagattgg tttggtcttg
    19621 cgttcgactg gggataatat cccgatgagt gaggccaatg gagtcaatgt agacaccatg
    19681 aagattgttg gttacatgat ttcaaacggt ttgattgccc tatgtggttc cttgtttgcc
    19741 caaaatgatg gattttcaga tgtgacttct gggacaggaa ccattgttgt tggtttgagt
    19801 tcggtgatta ttgcggaagt cttgatacac gacttgacca ttggaggccg cttattatcc
    19861 atcggaatcg gtgctattgt ttaccgtttg attattttaa atatctatga aattccaaat
    19921 ctagatcaaa acttggtacg tctctttaat gcaatcttgc tcgctttggt tttatttgca
    19981 ccagaattgc aaaagagatt aaagattcgt ggtctgaaat tgagaaatga ataggaggag
    20041 aaagttatgg caagtttgtt aacacttgaa aatattcaca agacatttga agcagggacg
    20101 gtcaatgaga accatgtcct caaaggatta gatttagagg ttgaagaggg agattttatc
    20161 tctgtgattg gtggaaatgg agcaggaaaa tccactttga tgaatatctt ggctggcaat
    20221 ctatcgatgg acgaagggga ccttctactg gcagggaaat ccattaaaaa tctgagtgtc
    20281 agaaagagag ccaaggatat tgcccgtgtt tttcaagctc ctaagatggg aacagcttct
    20341 cgtttgacga ttgaggagaa tatggctatt gccttgagac gtgggcaaaa gagagggctt
    20401 ggttggggcg tgaaggagaa ggatagaatc cagttccaag aggccttgaa ggagttgaat
    20461 attggtcttg aaaaccgctt aaaagtagat actcaatatc tctcaggagg acaaagacag
    20521 gctttgaccc tagtcatggc agctctggtg aaacccaaac ttttgctttt ggatgaacat
    20581 actgcggcac ttgacccgaa aaccagtcaa atggtgatgg acttgacgca aaagattgtg
    20641 gagcaccatc agttgacgac tttgatgatt acccatgata tgaatcatgc gattgagtac
    20701 ggcaatcgtc tcattatgct ctatcaaggc aagatagtgg tggatgtcaa gggagaagag
    20761 aaaaagcatc tgacagttga agaccttatg catctcttcc agaaaaatag tggccaaagt
    20821 ctggtcagtg atgaattagt tttgagataa agaaaaaggc tgagattggt gtctcagtct
    20881 tttatttctt gcgttttgca aaatctacaa atctaaattt ttctaactta tggcgacttt
    20941 cggtaaactg aaattggcgt ccgtcttgga gatagacttt tgatttaatc gaaacgacat
    21001 aagggtcttt gccaatgtcc atgagaatct tgtctcggtc gcttgagtgg tcaatggtga
    21061 tttccttctg agcataatca ataaggagtt tgaggccatt ttctatataa gaatagatag
    21121 actgctcagc aatttggcga gtgagatttg ggatgagttc catatccaga tagtctgtat
    21181 ccagaacgga taccagatca tccaccacac gctggcggac caccttccaa accatccgaa
    21241 actctgggaa accggttatc agtgaggatt ttttatcaat aataatcttg tccagactga
    21301 ccacgttggt tttagagcgc agtccaagtt ctttaactag ttcttggtag ctggttaggt
    21361 tggatacagg gaaattgacg gtttcttctt tgacgacttg agaaccttgc cctcttatct
    21421 ttttgatcaa tccttcctct tggaggagag acagggcctt tcggacggta tcacgactca
    21481 cttgatattg ctccataagg tcgtgctcgc taggaaggaa atctccgaca gcgtaagtct
    21541 cgttttgaat ggtttcttgg atttgcttaa ataattgttg gtatttcttc attttgcctg
    21601 cttaaatagg tttttagtct agtttaatga acacctgtat ctattttacc atacaagtgc
    21661 tagaaaattt acaaaaaaac acccttttta taccaaacaa gttgcagaca agtttggtat
    21721 cgtttacaat atacttatca aatcaaactt gctttgacaa gtataaggag aatcaaatgg
    21781 gaaaatttga acaagaagcc aaagatctgc ttcaggcaat tggaggcaaa gaaaatgtga
    21841 ctgccgtcac ccactgtgcg acacggatgc gctttgtttt aggagatgat aagaaggcta
    21901 atgttaaagc tatcgagtca attccagctg ttaaaggaac ttttacaaat gcaggtcaat
    21961 ttcaggtaat cattggaaat gacgtgccca tcttttataa tgattttaca gccgtttcag
    22021 gtattgaggg tgtttccaaa gaagcagcca agtctgcagc taagagtaat caaaacgtgg
    22081 tccaacgtgt tatgaccact ctggcggaga tttttactcc gattattcca gccttgatag
    22141 tcggaggatt gatcctcggt ttccgtaatg tcttggaagg tgtccattgg tcgatgttgg
    22201 atggcaagac catcacagaa tcctctcagt tttgggcagg tgtcaatcac ttcctctggt
    22261 tgcctggtga agctatcttc cagttcttac cagtagggat tacttggtct gtttctcgta
    22321 agatgggaac cagccaaatt ttgggaattg ttctcggaat ctgtttggta tcgcctcagt
    22381 tgctcaatgc ctatgcggtt gcttcaacgc cagcagctga tatcgcggca aactgggttt
    22441 ggaattttgg ctattttact gttaatcgta tcggttacca agcccaagtt atcccagcct
    22501 tgcttgcagg tttgagtctg tcttatcttg aaatcttctg gcgcaagcat atcccagaag
    22561 tcatttctat gatttttgta cctttcttgt cattgattcc agccttgatt ttggctcata
    22621 ctgttttggg accaatcggt tggacaattg gacaaggact ttcatcagtt gtcttggcag
    22681 gtttaactgg tccagttaaa tggctcttcg gtgcaatttt tggtgccctc tacgctccat
    22741 ttgtcatcac aggtctgcac catatgacca atgccattga tacacaattg attgcggatg
    22801 ctggtggcac tgccctatgg ccaatgattg ctctttctaa tattgctcaa ggctcagccg
    22861 tgtttgccta ttatttcatg catcgccatg atgagcgtga ggctcaggtt tcacttcctg
    22921 caaccatttc agcctatctc ggtgttacag aaccagctct ttttggggtt aacgtaaaat
    22981 atatttatcc atttgttgct gggatgactg gttcagccct tgcaggcatg ttatccgtta
    23041 cttttaatgt aactgcggct tctattggta tcggtggttt gccaggtatt ctctctattc
    23101 aacctcaata catgctgcca tttgcaggaa ctatgctagt tgcgattgtt gttccaatgc
    23161 tcttgacttt cttcttccgc aaggctggtc tctttacaaa aacagagggc gatacgaact
    23221 tgcaggcaga attcgttgct caagaagaag cagaatttgt gaaccatgaa ccagtagaac
    23281 ttacttcggt agaaattatc agcccactaa ctggccaagt gaaagaattg agtcaagcga
    23341 cggatcctat ttttgcatca ggtgtcatgg ggcaaggtct agtcattgaa ccaagccaag
    23401 gtgagttgac ctctccagtt aatgggacag tgacggttct tttccctacc aagcatgcca
    23461 tcggcattgt ctctgacgag ggagttgaat tgctcatcca catcggtatg gatacagtag
    23521 gtcttgatgg caaaggtttt gaaagtcttg tagtccaagg agatcacgtt acagttggtc
    23581 agcaactgat tcgttttgat atggatgtca ttaaggctgc aggtctggtg acagaaactc
    23641 ctgttatcat caccaaccaa gatgcttata cagcgactat tcccggaact tatccgacaa
    23701 cgatccaagc tggagcatct ctcatggtcg ctacacgaat ctaatactct tcgaaaatct
    23761 cttcaaacca cgtcagctct atctgcaacc tcaaaatagt gttttgagca acctgtggct
    23821 agcttcctag tttgttcttt gattttcatt gagtataatt gaaatagcac aaatagggac
    23881 ggaagactgc ttcctccctt ttatcagaaa aggagaaaca gatgacactt gataaaggaa
    23941 aagtagtcta tcaaatctat ccaaaatctt acaaagacac cactgaaaac ggtattgggg
    24001 atttccgtgg gattatcgaa aaaattccct atttagccaa actaggtgtg gatatggtct
    24061 ggctcaatcc attctatcca agccctcaac gggataatgg ttacgatatt tcagattata
    24121 tggcagtgga tcctcttttt ggtgatatgg ctgattttga ggaaatggtg tgtgtcggta
    24181 aagagcacaa gattgacttt atgctggaca tggtacttaa tcattgttcg acagagcatg
    24241 aatggtttca gaaagcccta gctggtgata agtattatca agactttttc ttcatccaag
    24301 accaaccaac agattggcag tctaagtttg gtggttctgc atgggcgcct ttcggggata
    24361 ctgggaaata ctatcttcac ctatttgatg agacccaggc tgaccttaac tggcgcaatt
    24421 ccaatgtccg taaggagctt ttcaaggttg ttaatttctg gcgcgacaag ggtgtcaaag
    24481 gtttccgatt tgatgtgatc aatttgatcg gtaaggacga ggtttcagtg gactgtcctg
    24541 aaaatgaagg gaagccagct tatacagaca aacccatcgt tcataactat ttgcgtatga
    24601 tgaaccaagc cacttttgga tctgacgata gctttatgac agttggggaa atgtcttcta
    24661 ccaccatgga aaactgtgtc ctctattcgt cgcctgatcg tcaggaatta tccatgacct
    24721 ttaattttca tcacctcaag gtggactata aggatggaca aaagtggacc ttggctccct
    24781 ttgattttga agagttgaag agtctttacc atagttgggg taaggaaatg agtgataaag
    24841 acggttggag tgccctcttt tggaacaatc acgatcaacc acgtgccttg aaccgttttg
    24901 tcgatattca aaacttccgc aaggaaggag ctaccatgct agcagccagc attcacctgt
    24961 cacgtgggac accttatatc tacatgggtg aggaaatcgg gatgattgac ccagactatg
    25021 attctatggc tgattatgtg gatgtcgaat cgcttaatgc ctatcagatg ctcttagaag
    25081 aaggaaagag tcagcaagaa gccttccaga ttattcaagc taagtcgcgt gataattcac
    25141 gtattcccat gcagtgggat acttctgaaa atgcaggatt ttcaacaggc actccatggt
    25201 tgaaagttgg taaatcctat aaagatatca atgtagaaaa tgaaatcaaa ggtctgattt
    25261 tcaccttcta tcaagacttg attcgactgc gtaaggaaat gccaatcatt tcagaaggaa
    25321 attacaaacc agcctttgaa gatagcaaac aagtctacgc ttttgaacgc cagtttgagg
    25381 atcaaaagtt actagtgctc aataactttt atgccaaaga agtggaaatc gacttgccag
    25441 cagtctacca aaatggacag attttgattt caaactatga agatgcagaa gtgtctgaga
    25501 aaattctact caaaccctat caaacactgg ctatttatgt aaattaatac tcttcgaaaa
    25561 tctcttcaaa ccgtgtcagt tttatctgta acctcaaagc ggtgctttga gcaacctgtg
    25621 gctagcttcc tagtttgctc tttgattttc attgagtata aacttagcaa ggagacctct
    25681 ttttcagaaa agcagggttc tttttgctgt ctattccatc cttgaaagtg tagaacaaac
    25741 tggtcagggt ggctagtttg tggtataatg aaagggactc aaaaagaaac aggagaaaaa
    25801 tgatggattt acttttagca attgtattga ttgtgctagc ttttctagga ggagctcttg
    25861 gaggaatgta cttggttcgt aagcaaatcg aaaaagaatt tgctgacaac ccacgtttga
    25921 atgctgaagc agttcgtact cttttgagtg caaatggtca aaaaccaagc gaagctaagg
    25981 tacaacaagt ttaccaccaa atcatccgcc aacaaaaggc agcccttgct aacaataaaa
    26041 agaaaaaata aataaggaaa agtctgggat gaaagttcca gacttctcac tatgttggtt
    26101 atggtttagg gatgagactt tttccttgca ttctattgat atttgattat gattaagaga
    26161 gaggtagttg acattctatc aagctgtcgg tcagttcgtt cagtacaagg aatcataatg
    26221 aacgatcaat cagaaaaaag actagaaaga agactgtatg gataatcgac caattggttt
    26281 tttggattcg ggtgtcgggg gcttgaccgt tgtgcgcgag ctcatgcgcc agcttcccca
    26341 tgaagaaatc gtctatattg gagattcggc gcgggcgccc tatggccccc gtcctgctga
    26401 gcaaattcgt gaatatactt ggcagctggt caactttctc ttgaccaagg atgtcaaaat
    26461 gattgtcatt gcttgtaaca ctgcgactgc ggtcgtctgg gaagaaatca aggctcaact
    26521 agatattcct gtcttgggtg taattttgcc aggagcttcg gcagccatca agtccagtca
    26581 aggtgggaaa atcggagtga ttggaacgcc catgacggta caatcagaca tataccgtca
    26641 gaaaatccat gatctggatc ccgacttaca ggtggagagc ttggcctgtc ccaagtttgc
    26701 tcccttggtt gagtcaggtg ccctgtcaac cagtgttacc aagaaggtgg tctatgaaac
    26761 cctgcgtccc ttggttggaa aggtggatag cctgattttg ggctgtactc attatccact
    26821 ccttcgccct attatccaaa atgtgatggg gccaaaggtt cagctcatcg atagtggggc
    26881 agagtgcgta cgggatatct cagtcttact caattatttt gaaatcaatc gtggtcgcga
    26941 tgctggacca ctccatcacc gtttttacac aacagccagt agccaaagtt ttgcacaaat
    27001 tggtgaagaa tggctggaaa aagagattca tgtggagcat gtagaattat gacaaataaa
    27061 atttatgaat ataaggatga ccaggactgg tatgttgggt cttatagtat ttttggtggc
    27121 gttaacagtt tgagcgacta taagacagat tttcctctgt ttgaattctc caaaatattt
    27181 ggagatgaag agtatggttt cccgctttca gttactgttt tacgctatgg ttctacctac
    27241 cgtttgttct cctttgtggt agacatgctt aatcaagaaa tgggacgaaa cttggaagtt
    27301 attcaacgtc atggggccct gctcttggtt gaaaatgggc aactcttgta tgtagaattg
    27361 cctaaagaag gggtcaatgt tcatgatttc tttgagacaa gcaaggtcag agaaaccttg
    27421 ttgattgcga ctcgtaacga aggtaaaacc aaggaattcc gagctatctt tgataagtta
    27481 ggctacgatg tggaaaatct taatgactac cctgacctgc ctgaagtagc agaaacaggt
    27541 atgacctttg aagaaaatgc ccgccttaag gcagaaacca tttctcaatt aacgggcaag
    27601 atggttttgg cagatgattc tggtctcaaa gtcgatgtcc ttggtggctt accaggcgtc
    27661 tggtcagctc gtttcgcagg tgtgggagca actgaccgtg aaaataatgc caaactcttg
    27721 cacgaattgg ccatggtctt tgaactcaag gaccgctcgg ctcagttcca cacaacccta
    27781 gtcgtagcca gcccaaataa ggaaagttta gttgttgaag cagactggtc aggttatatt
    27841 aactttgaac ctaagggtga aaatggcttt ggctatgatc ccctcttcct tgtaggagag
    27901 acaggtgagt catcagctga attaaccctg gaagaaaaaa atagtcaatc tcaccgtgcc
    27961 ttagccgtta agaaactttt ggaggtattt ccatcatggc aaagcaaacc atcattgtaa
    28021 tgagcgattc ccatggcgat agcttgattg tggaagaagt ccgtgatcgc tatgtgggca
    28081 aagtcgatgc cgtttttcat aacggcgatt ctgaactacg tccggattct ccactttggg
    28141 agggcatccg cgttgttaaa gggaacatgg acttctacgc cggctaccca gaacgtctgg
    28201 tgactgagct tggttcgacc aagattatcc aaactcatgg tcacttgttt gacatcaatt
    28261 tcaactttca aaagttggac tactgggctc aggaggaaga ggccgctatc tgcctctatg
    28321 gtcacttgca tgtgccaagt gcttggatgg aaggcaagat cctctttcta aatccaggtt
    28381 ctatcagcca accacgaggt accatcagag aatgtctcta tgctcgtgtg gagattgatg
    28441 atagttactt caaagtggac tttttgacac gagatcacga ggtgtatcca ggtttgtcca
    28501 aggagtttag ccgatgattg ccaaggagtt tgagactttc ttgttggggc aggaggaaac
    28561 ttttttgacc cctgctaaaa atctagctgt gttgattgat acccacaatg cggatcatgc
    28621 gaccctcttg ctcagtcaga tgacctatac ccgtgttccc gttgtgacag atgaaaaaca
    28681 gtttgttggg acgattggac tcagagatat tatggcttat cagatggagc atgacttgag
    28741 ccaagaaatc atggcggata cggatatcgt tcatatgaca aaaacggacg tagcggttgt
    28801 ttcgcctgat ttcaccatta cggaggtctt gcacaagcta gtagatgagt ccttcttacc
    28861 ggttgtggat gcagagggta ttttccaagg gattattacg cgcaagtcca tcctcaaggc
    28921 cgttaatgcc ctcttgcatg actttagtaa ggaatatgag attcgatgcc aatgagagac
    28981 aggatttcag cctttttaga ggaaaagcag ggcttgtctg tcaattccaa gcagtcctat
    29041 aagtatgatt tggagcaatt tttagacatg gtaggtgagc ggatttctga gaccagtctc
    29101 aagatttacc aagcccagct agccaatcta aaaatcagcg cccagaagcg aaagatttcg
    29161 gcctgtaacc aatttctata ctttctctat caaaaaggag aggtggacag cttttaccgc
    29221 ttggaattag ccaaacaagc tgaaaagaag acggaaaagc cagagattct atacctagac
    29281 tctttttggc aggaaagcga ccatccagag ggccgcttgc tagcgctctt aatcctagaa
    29341 atggggctct tgcccagtga gattttagcc atcaaggttg cggacatcaa tctggatttt
    29401 caggtgttgc gaatcagcaa ggcttcccaa cagaggattg tcaccattcc cacggccttg
    29461 ctttcagaat tggaaccctt gatggggcag acctatcttt ttgaaagagg agagaaaccc
    29521 tattctcgtc agtgggcctt tcgtcagtta gaatcttttg tcaaggagaa aggttttcca
    29581 tccttatcag ctcaagtctt acgtgaacag tttattctaa gacaaataga aaacaaggtc
    29641 gatttgtacg aaattgcaaa aaaattagga ttaaaaacag tcctgacctt agaaaaatat
    29701 agataatgga tattaaatta aaagattttg aaggacccct ggacttgctc ttgcatctgg
    29761 tttctaagta ccagatggat atctacgatg tgcccattac ggaagtcatc gaacagtatc
    29821 tagcctatgt ctcaaccctg caggccatgc gtctggaagt gacgggtgag tacatggtca
    29881 tggctagtca gctcatgctg attaagagtc gtaaactcct tccgaaggta gcagaagtga
    29941 cagacttggg ggatgacctg gagcaggacc tcctctctca aatcgaagaa tatcgcaagt
    30001 tcaagctctt gggtgagcac ttggaagcca agcaccaaga acgggcccag tattattcca
    30061 aagcgccgac agagttgatt tacgaagatg cggagcttgt gcatgacaag acgaccattg
    30121 acctcttttt ggctttttca aatatcctag ccaagaaaaa agaggagttt gcacaaaatc
    30181 acacgacgat cttgcgggat gagtataaga ttgaggacat gatgattatc gtgaaagagt
    30241 ccttgattgg acgagatcaa ttgcgcttgc aggatttgtt caaggaagcc cagaatgtcc
    30301 aagaggtcat caccctcttt ttggcaaccc tagagttaat caaaacccag gagctgatcc
    30361 tcgtgcaaga ggagagtttt ggagatatct atctcatgga aaagaaggaa gaaagtcaag
    30421 tgcctcaaag ctagacttga tagagaggaa agatgagtac tttagcaaaa atagaagcgc
    30481 tcttgtttgt agcgggtgaa gatgggattc gggtccgcca gttagctgaa ctcctctctc
    30541 tgccaccgac aggcatccag caaagtttag gaaaattagc ccagaagtat gaaaaggacc
    30601 cagattccag tttggctttg attgagacaa gtggtgctta tagattggtg accaagcctc
    30661 aatttgcaga gattttgaag gaatactcta aggcgcctat caaccagagc ttgtctcggg
    30721 ctgcccttga gaccttgtcc attattgcct acaaacagcc gattacgcgg atagaaattg
    30781 atgccatccg tggagttaac tcgagtggag ccttggcaaa gttgcaggct tttgacctga
    30841 taaaggaaga cgggaaaaag gaagtattgg ggcgccccaa cctctatgtg actacagatt
    30901 atttcctaga ttacatgggg ataaaccatt tagaagaatt accagtgatt gatgagcttg
    30961 agattcaagc ccaagaaagc caattatttg gtgaaaggat agaagaagat gagaatcaat
    31021 aagtatattg cccacgcagg tgtggccagt aggagaaaag cagaagagct gattaagcaa
    31081 ggcttggtga cggttaacgg tcaagtggtg cgtgaactag caaccactat caagtcaggc
    31141 gacaaggtcg aagttgaagg tcaacctatc tacaacgaag aaaaggtcta ctatctgctt
    31201 aacaaaccac gcggtgtcat ttccagtgtg acagatgata agggtcgcaa gacggttgtc
    31261 gacctcttgc ccaatgtcaa agagcgtatt taccctgtgg gtcgtttgga ctgggataca
    31321 tcaggagtct tgattttgac caatgatggg gactttacag acgagatgat tcaccctcgt
    31381 aatgagattg acaaggttta tgtcgcgcgt gttaaaggtg tggccaataa ggacaatctt
    31441 cgccccttga cccgtggtct tgagattgat ggtaagaaaa ccaagccagc tgtttatgaa
    31501 attctcaaag tggacccagt caaaaatcgc tctgtggtgc agttgaccat ccatgaaggg
    31561 cgtaaccatc aggttaaaaa gatgtttgaa gctgttggtc tccaagtaga taagttgtct
    31621 cggactcgtt tcggacacct agacttgaca ggactccgtc caggagaatc ccgtcgtctt
    31681 aataaaaaag aaatcagcca actacacacc atggctgtaa ctaagaaata atgaaacgaa
    31741 ttttaatagc gcttgtgcgc ttttaccaac gttttatctc accagtcttt ccaccctctt
    31801 gtcgctttga gctgacttgt tccaactaca tgattcaggc tattgaaaaa catggtttta
    31861 agggggtatt gatgggcttg gctcggattt tacgttgtca tccctggtcg aaaacaggta
    31921 aggaccccat tccagaccac ttttccctta aacgaaatca agaaggggaa tgaggtgggg
    31981 taaatagatt tcaaaatgat aaaaacgcat cctatcaggt ttgagtaaac ttgataggat
    32041 gcgttttaga atgtcaaaat tttatactct tcgaaaatct cttcaaaccg cgtcagcttt
    32101 catctgcaac ctcaaaacag tgttttgagc aacctgcggc tagtttccta gtttgctc
//
LOCUS       DBERDK010000020        27846 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.020, whole genome shotgun sequence.
ACCESSION   DBERDK010000020 DBERDK010000000
VERSION     DBERDK010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 27846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27846
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.020"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            83..880
                     /locus_tag="ACL73L_000873"
     CDS             83..880
                     /locus_tag="ACL73L_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HGS0771875.1"
                     /translation="MISSEFISKIEFACNKKESLYSQSKFKYAIRSMFAGAFLTFSTA
                     AGAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSFLKKISW
                     RKTAEILLYCTLFNLIGALIAGWGFAHSAAYANLTHDSFISGVVEMKLGRSNELVLLE
                     AILANIFVNIAILSFILVKDGGAKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVA
                     ADSIANFGVGNMLRHWGVTFIGNFIGGGLLMGLPYAFLNKNEDTYVD"
     gene            1028..2287
                     /locus_tag="ACL73L_000874"
                     /pseudo
     CDS             1028..2287
                     /locus_tag="ACL73L_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365067.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
     gene            2383..3657
                     /locus_tag="ACL73L_000875"
     CDS             2383..3657
                     /locus_tag="ACL73L_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HGS0771876.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKELHDRMRQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHQALLAKDKEV
                     QALENQLATLRLEHENQLQKTLSDLEKERNQVKNQLLLQEKENELSLASVKQNYEAQL
                     KAASEQVEFYKNFKAQQSTKAIGESLEQYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRQEKNCEYAVLV
                     TMLEADNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            3667..4545
                     /gene="truB"
                     /locus_tag="ACL73L_000876"
     CDS             3667..4545
                     /gene="truB"
                     /locus_tag="ACL73L_000876"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HGS0771877.1"
                     /translation="MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAVGKATRMVEFMQDEGKIYEGEITLGYSTTTEDASGEVIAETPVLSPLYEKLVDE
                     AIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVIIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALSLEEIAE
                     KVEAGQLDFLHPLEIGTGDLVKVFLTPEEATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
     gene            4786..5088
                     /locus_tag="ACL73L_000877"
     CDS             4786..5088
                     /locus_tag="ACL73L_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HGS0771878.1"
                     /translation="MMRNEFRERVEQLLQQKEINENSELSHLFRLAIQNLDRNEKYQS
                     VMANLSQGLSLYLMTHHYQAPKSVIDFGLWIAKAPSQERGRLAFLQMLAQTLQGFR"
     gene            5196..5834
                     /gene="udk"
                     /locus_tag="ACL73L_000878"
     CDS             5196..5834
                     /gene="udk"
                     /locus_tag="ACL73L_000878"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HGS0771879.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            5962..7302
                     /gene="xseA"
                     /locus_tag="ACL73L_000879"
     CDS             5962..7302
                     /gene="xseA"
                     /locus_tag="ACL73L_000879"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HGS0771880.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDDHAVIQATIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKAEPDG
                     VGALAIQFEQLKKKLTEEDLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEGAAEEIARNIARANQRDDLDLLIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLAHLQNQEK
                     RMATAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     KQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLGSQMALVYDAKVAEAKRLSEALLM
                     LDTSRIVARGYAIVKKEESVVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     gene            7280..7492
                     /locus_tag="ACL73L_000880"
     CDS             7280..7492
                     /locus_tag="ACL73L_000880"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HGS0771881.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            7489..8364
                     /locus_tag="ACL73L_000881"
     CDS             7489..8364
                     /locus_tag="ACL73L_000881"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HGS0771882.1"
                     /translation="MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLEALQVAIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDPYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQTIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQIPFE
                     TESIVSVVESLRING"
     gene            8357..9172
                     /locus_tag="ACL73L_000882"
     CDS             8357..9172
                     /locus_tag="ACL73L_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HGS0771883.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVLNGERFDKPG
                     EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVDGATTIDIGASTGGFTDVMLQ
                     NSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISL
                     SLILPALHRVLADQGQVVALVKPQFEAGREQIGKNGIIRDAKVHQNVLESVTAMAVEV
                     GFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQILAEIKEAVERAHSQFKNE"
     gene            9165..9596
                     /locus_tag="ACL73L_000883"
     CDS             9165..9596
                     /locus_tag="ACL73L_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HGS0771884.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVK
                     AQLIETFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            9603..11270
                     /gene="recN"
                     /locus_tag="ACL73L_000884"
     CDS             9603..11270
                     /gene="recN"
                     /locus_tag="ACL73L_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HGS0771885.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIFDKQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRAIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKARIEMLEFQMAEIEAANLQAGEDLALNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSYLSETYY
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTVDDVLLYFAKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKA
                     QFQVRFSKGKFSREGNKMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIHKIGQHGQVLAISHLPQVIAIADYQFFIEKIS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK"
     gene            11272..12000
                     /locus_tag="ACL73L_000885"
     CDS             11272..12000
                     /locus_tag="ACL73L_000885"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HGS0771886.1"
                     /translation="MTDYYVIGDVHGKAGMLEDLLKTWDGQPQLLFLGDLIDRGEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDDPEESYDHYSRNGGDTTINSILGRPLD
                     APVDGVEDAKRVAAEASDLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHGAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLH
                     GIVFTDQGMTEHHFIENDGFVAED"
     gene            12079..13902
                     /gene="lepA"
                     /locus_tag="ACL73L_000886"
     CDS             12079..13902
                     /gene="lepA"
                     /locus_tag="ACL73L_000886"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HGS0771887.1"
                     /translation="MNLEELKKRQGKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAADPERVRTEIEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQ
                     VRVMDGVVKPGDKIQLMSNSKTFDVAEVGIFTPKAVGRDFLATGDVGYIAASIKTVQD
                     TRVGDTVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMD
                     VSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFI
                     VHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE"
     gene            complement(13983..14270)
                     /locus_tag="ACL73L_000887"
     CDS             complement(13983..14270)
                     /locus_tag="ACL73L_000887"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771888.1"
                     /translation="MKIKEQTRKLAAGYSKHSFEVVDKTDEVSSKHDFEVVDETDEVS
                     SKHDFEVVDETDEVSSKHGSEVVDETDEVSNHTYGKATLTWFEEIFEEYKN"
     gene            14450..14926
                     /locus_tag="ACL73L_000888"
     CDS             14450..14926
                     /locus_tag="ACL73L_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGS0771889.1"
                     /translation="MVLDYFFDKNLVFCLEADNQEQLFDQVASLLEEREIVTPTYREA
                     LITREKSFPTGLDMEFLGKDLPNVAIPHTDIVHNLAEKVVVVRLEKPVTFHNMIAPDK
                     EVEVSLLFFIINNSSSSQTNILAQLMDFFTGNGHLEDLSKISEPEALYAYIAEATA"
     gene            14964..15265
                     /locus_tag="ACL73L_000889"
                     /pseudo
     CDS             14964..15265
                     /locus_tag="ACL73L_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
     gene            15273..16076
                     /locus_tag="ACL73L_000890"
                     /pseudo
     CDS             15273..16076
                     /locus_tag="ACL73L_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            16267..18558
                     /gene="pcrA"
                     /locus_tag="ACL73L_000891"
     CDS             16267..18558
                     /gene="pcrA"
                     /locus_tag="ACL73L_000891"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HGS0771890.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNPWNILAITFTNKAAREMKERAYSLNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKWNERTILGTISNAKNDLIDDVAYAAQA
                     GDMYTQIVAQCYTAYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNLWTQNADGEQIVYYRADDELDEAVFVART
                     IDELSRSQNFLHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGLGTVEKIRDFANLQNMSMLDASANIMLSGIKG
                     KAAQSIWDFANMMLDLREQLDHLSITELVESVLEKTGYVDILNAQATLESKARVENIE
                     EFLSVTKNFDDTTDVTEEETGLDKLSRFLNDLALIADTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDSDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     GAAPKSIQSSGLPFGQFTAGAKPASSEANWSIGDIALHKKWGEGTVLEVSGSGARQEL
                     KINFPEVGLKKLLASVAPIEKKI"
     gene            18601..19281
                     /gene="radC"
                     /locus_tag="ACL73L_000892"
     CDS             18601..19281
                     /gene="radC"
                     /locus_tag="ACL73L_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HGS0771891.1"
                     /translation="MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQASV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHPSGAVAPSQNDDHVTKLVKEACELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(19278..19967)
                     /locus_tag="ACL73L_000893"
     CDS             complement(19278..19967)
                     /locus_tag="ACL73L_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HGS0771892.1"
                     /translation="MKKPVIGITGNEKTHPDDDIMMSYAAKGFVEGVKDAGGIPIILP
                     IGDQEMACHYISLIDKLILTGGQNVDPKFYGEPKTIDSDDYHLQRDIFELALIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPELLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(19985..20626)
                     /locus_tag="ACL73L_000894"
     CDS             complement(19985..20626)
                     /locus_tag="ACL73L_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HGS0771893.1"
                     /translation="MKDKQFAIPKATAKRLSLYYRIFKRFHAEKIERANSKQIAEAIG
                     IDSATVRRDFSYFGELGRRGFGYDVKKLMTFFADLLNDNSITNVMLVGIGNMGHALLH
                     YRFHERNKMKIIMAFDLDDHPEVGTQTPDGIPIYGISQIKDKIKDADVKTAILTVPSV
                     KSQEVANLLVDAGVKGILSFSPVHLHLPKDVVVQYVDLTSELQTLLYFMRKED"
     gene            complement(20709..20924)
                     /locus_tag="ACL73L_000895"
     CDS             complement(20709..20924)
                     /locus_tag="ACL73L_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HGS0771894.1"
                     /translation="MFELTYKDCYHVERTLKYEDHEALMLTLSGCVTLPDTLYVTSLT
                     FRGKKVYQGLVGDLYRFLSHADFLHQN"
     gene            complement(20932..21279)
                     /locus_tag="ACL73L_000896"
     CDS             complement(20932..21279)
                     /locus_tag="ACL73L_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HGS0771895.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKELTGAKINITDKFGLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            complement(21284..22399)
                     /locus_tag="ACL73L_000897"
     CDS             complement(21284..22399)
                     /locus_tag="ACL73L_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HGS0771896.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLKAMY
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            complement(22409..23368)
                     /locus_tag="ACL73L_000898"
     CDS             complement(22409..23368)
                     /locus_tag="ACL73L_000898"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HGS0771897.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
     gene            complement(23481..24050)
                     /locus_tag="ACL73L_000899"
     CDS             complement(23481..24050)
                     /locus_tag="ACL73L_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HGS0771898.1"
                     /translation="MKHLEIELKTLLKKDEYNRLKDQFTGVTPVLQTNYYIDTPDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAENYLSKEELPQGLVLDEL
                     AKHGIQSKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDITDYELELEVENHEQGKQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
     gene            24178..24849
                     /locus_tag="ACL73L_000900"
     CDS             24178..24849
                     /locus_tag="ACL73L_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HGS0771899.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRGITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGVKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
     gene            24833..25651
                     /locus_tag="ACL73L_000901"
     CDS             24833..25651
                     /locus_tag="ACL73L_000901"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HGS0771900.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLEKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIERIEYQIAHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            25648..26544
                     /locus_tag="ACL73L_000902"
     CDS             25648..26544
                     /locus_tag="ACL73L_000902"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HGS0771901.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVTIDIPAEKGFETLEAIELPLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGDGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     YIHLHTGRTHQIRVHFSHIGFPLLGDDLYGGSLEDGIQRQALHCHYLSFYHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            26588..27562
                     /gene="pta"
                     /locus_tag="ACL73L_000903"
     CDS             26588..27562
                     /gene="pta"
                     /locus_tag="ACL73L_000903"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HGS0771902.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPV
                     LLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVE
                     DVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
                     YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVE
                     ATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYK
                     MAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
BASE COUNT         8265 a         5186 c         6274 g         8121 t
ORIGIN      
        1 acttttatga aatacagaaa acgcttccta aaacctaaag tttgtgatat aattatcaag
       61 aaaaaagatt taggagttta ttatgatttc ttcagaattt atctcaaaga ttgaatttgc
      121 ttgcaataag aaagaaagtc tttatagtca aagcaaattt aagtatgcga ttcgttcgat
      181 gttcgcaggt gcatttttaa ccttcagtac tgctgcaggt gcagttgggg ctgacttgat
      241 taataaaatt gcaccaggta gtggacgctt cctctttcca ttcgtttttg cttggggctt
      301 ggcctacatt gtttttttga atgccgagtt ggtcacttca aacatgatgt tcttgactgc
      361 tggtagtttc ttaaaaaaaa tctcttggag aaaaacagct gagattttac tatactgtac
      421 cttgttcaac cttatcggag ccttgatagc agggtggggc tttgctcatt cggcagccta
      481 tgcgaatctg acacacgata gtttcatctc aggtgttgtt gagatgaagt taggccgctc
      541 aaatgaattg gtcttgcttg aggcgatttt ggcaaatatt ttcgtaaata ttgcgattct
      601 gtcatttatt ttggtcaaag atggtggtgc caaactttgg cttgtgttgt cagctattta
      661 catgtttgta ttcttaacaa acgagcacat tgcggcgaac tttgcttctt tcgcgattgt
      721 gaaattcagt gttgctgcgg attcaattgc caacttcggt gttggaaata tgcttcgcca
      781 ctggggtgtg actttcatcg gaaactttat cggaggaggc ctcttgatgg gtcttccata
      841 tgccttcctc aataaaaacg aagatactta tgtagattaa gaaaatgagc acgattgagt
      901 cgtgcttttt tcattttcaa aataaggtaa tagctatttc ttatatcaaa atatagaaaa
      961 ctgatatttg taaactataa ctcaaggtgc tacaatatcc ttaataaaat gatatggagg
     1021 tcaccttatg acttgtgatt ttaaatctga aactctacaa ctacatgctg gtcaagttgt
     1081 ggctccagct actaagtctc gtgcagtgcc gatttatcaa acaacatttt ttgtttttga
     1141 tgacacgtag gaaggtgccg atctgtttgc cttgaggaaa ccagggaaca tttatactcg
     1201 tatcaccaat cctacaacag ctgcccttga aggtggtgtt gaagcgctag caacagcatc
     1261 aggtatgact gcagtgactt atacgatttt ggcgcttgcc catgctggtg accatgtagt
     1321 ggctgcttcg actatttacg gtggaacctt caatcttttg aaagaacccc ttcctcgtta
     1381 tggtatcaca acaacctttg tcgatattga taatttggag gaagtagaag cagctatcaa
     1441 agacaatacc aagcttgtct tgattgaaac cttgggtaac cccttgatta atattccaga
     1501 cctggaaaaa ctggcagaga ttgctcataa acatcaaatc ccacttgtgt cagacaatac
     1561 ttttgcaaca ccttatttga ttaacgtctt ctctcatggc gttgacattg ccattcactc
     1621 tgcgactaag tttatcggtg ggcatggtac aactattgga gaaataattg tcgatagtgg
     1681 tcgttttgac tggacggctt cagggaaatt ccctcaattt gttgacgagg gtccaagctg
     1741 ccacaatttg agttatactc gtgatgtggg tgcagcagcc tttattatag ctgttcgagt
     1801 tcaattgctt cgtgatacag gtgcagcctt gtcaccattc aatgctttcc tcttgctaca
     1861 aagacttgaa accctttcac ttcgtgtgga acgccatgta caaaatgctg agacaattgt
     1921 tgattttctt gtcaaccatc ctaaggtaga aaaggtaaat tatccaaaac ttgcagatag
     1981 tccttattat gccttggctg agaaatactt gccaaaaggt gtcggttcaa tctttacctt
     2041 ccacgtcaaa ggtgacgagg aagaagcacg caaggtcatt gataatttag aaatcttttc
     2101 tgaccttgca aacgcggcag atgctaaatc gcttgttgtc catccagcaa caatcactca
     2161 cggtcaattg tcagaaaaag acctagaagc agcaggtgtc acaccaaatc aaatccactt
     2221 gtctatcggt cttgaaaatg tagaagattt gattgaagac ttacgcttgg ccttggaaaa
     2281 aatttaaagt aaaagaagat aaacagtggg ttttgactca ctgtttttga ttttccctca
     2341 ggcatgatat aatggttaca gaagtctaga aagaggaatg taatgaacga aatcaaatgt
     2401 cccaactgtg gggaagtctt tacagtaaat gaaagccagt atgctgaact tttgtcccaa
     2461 gtgagaacgg cagagtttga taaggaacta cacgatagga tgaggcagga actggccttg
     2521 gctgagcaaa aggctatgaa tgagcaacag actaaactgg ctcagaagga tcaagaaatt
     2581 gcgcaattgc agagtcaaat ccaaaacttt gatacagaaa aagaattggc caagaaagaa
     2641 gttgaacaga caagccatca ggccttattg gctaaggaca aggaagtaca ggccttggaa
     2701 aaccagttgg cgaccttgcg tttggagcat gaaaatcaat tgcaaaagac cctttctgac
     2761 ctagaaaaag aacgcaatca ggtcaaaaat cagctcctac tgcaagaaaa ggaaaatgag
     2821 ttgtctttgg cttctgttaa gcaaaactac gaagcccagc tcaaagcagc cagtgaacaa
     2881 gtcgagtttt ataagaattt taaggctcaa caatctacaa aagcgattgg ggaaagccta
     2941 gaacagtatg cagagagtga gtttaacaag gttcgtagtt tcgcctttcc aaatgcttac
     3001 tttgagaagg ataacaaggt ctcttcgcgt gggtctaaag gggactttat cttccgtgag
     3061 tgtgatgaaa atggagttga aatcatttct atcatgtttg agatgaaaaa cgaagcggac
     3121 ggaacagaga agaagcacaa gaatgcagat ttttacaagg aattggacaa ggaccgtcag
     3181 gagaagaact gtgagtatgc cgttttggtg accatgcttg aggccgataa tgactacttt
     3241 aacacaggga ttgttgacgt cagccacgag tatgaaaaaa tgtatgttgt tcgtcctcaa
     3301 ttctttatcc agttgattgg tctcttacgt aatgcggcgc taaattccct aaaatacaag
     3361 caggagttgg ccttggttcg cgagcaaaat attgacatta cgcattttga ggaagatttg
     3421 gatgccttta agctagcttt tgctaagaac tataattcag cttcgactaa ctttggaaaa
     3481 gctattgatg aaatcgacaa ggccatcaaa cgcatggaag aggttaagaa attcctgact
     3541 acatctgaaa accaactccg tctagctaac aacaaattgg aagatgtctc tgttaaaaaa
     3601 ttgacccgga aaaatccaac aatgaaagcg aagttcgaag cactgaaggg ggagtagaaa
     3661 gcaaaaatga acggtattat caacttaaaa aaagaagcag gaatgacctc gcatgatgcg
     3721 gtttttaaac tgcgtaagat tttgggaacc aagaaaattg gtcatggtgg aaccttggat
     3781 ccggatgtgg tgggtgtttt gccgattgcg gttggcaagg cgacacgcat ggtcgagttt
     3841 atgcaggacg agggtaagat ctatgagggg gaaattactc tgggttattc cacgacgact
     3901 gaggatgcta gtggggaagt gatcgcagaa acccctgttt tgtctccctt gtatgaaaag
     3961 cttgttgatg aagcgattgc tagcttgact gggcctatta cccagattcc ccctatgtat
     4021 tcggcagtta aggttaatgg tcgcaagctc tatgagtatg cgcgtgctgg tcaggaagtg
     4081 gagcgtccag aacgtcaggt gatcatttat caatttgagc gaacaagtcc gatttcttat
     4141 gatggccaac ttgcccgatt cacttttcgt gtaaaatgca gtaaagggac ttacatccgt
     4201 actttgtcag ttgatttggg tgaaaagctt ggttatgcgg ctcatatgtc ccatttgact
     4261 cgtactagtg ctgctggctt acaattagaa gacgctcttt ccttggagga aattgctgaa
     4321 aaagtagagg ctgggcaatt agattttctc catcctttag agattgggac aggtgacctt
     4381 gtcaaagttt tcctaactcc agaagaggct acagaagttc gctttggtcg ttttattgag
     4441 ctagaccaaa cggacaaaga actggctgcc tttgaagatg ataaattgtt agccattcta
     4501 gaaaaacggg gcaatctcta taagccaagg aaggttttta gctagatcgt ttaggaataa
     4561 aaatcgggtg atagataaca attgcttgat aaaaccccat actaatagta gaatggtttt
     4621 gggaattata atattccaat tgttgcgagt tgtaggtact caaataatct atatagaaat
     4681 ttagaggtgt gaaatgaagc aatttaaaat tctttcagat aaatatttag agtccattac
     4741 aggttctggt gggaacttag gcccaggatt tggtgtgata attccatgat gcgaaatgag
     4801 tttcgagaaa gggtggagca acttcttcaa caaaaagaaa taaatgaaaa tagtgagttg
     4861 agtcacctgt ttcgtcttgc tatacaaaat ttagacagaa atgaaaaata ccaatcggtc
     4921 atggccaatt tgagtcaagg gttgtcactt tacctcatga cgcatcatta ccaggcacct
     4981 aagtctgtca ttgattttgg tttatggatt gccaaagctc ctagccagga aagagggaga
     5041 ctggctttct tgcagatgct tgctcaaacc ctacagggct ttcgttaaaa ctgtcgaaca
     5101 tcagagggaa gactctggtg ttttttgctg atgagactat aaattttgta aaaaatgtga
     5161 tagaatagac gacggataaa ataacggagg atagcatgca aaatagacca atcattattg
     5221 gagtgacagg tggttctggt ggtggtaaaa ccagtgtttc aagagccatt ttatcgcatt
     5281 ttcctgatga aaagatttcc atgattgagc atgattcata ctacaaggat cagtctcatt
     5341 tgacctttga agagcgtgtc aaaaccaact atgaccatcc ttttgccttt gatacagact
     5401 tgatgatcga gcagattaag gaattgttgg cagggcgtcc ggtggacatc ccgacctacg
     5461 actatacaga gcatacacgg agtagcaaga cctatcgtca ggagcctcaa gatgtcttta
     5521 tcgttgaggg cattttggtc ttggaggaca agcgtctgcg cgatttgatg gatatcaaga
     5581 tttttgtgga tacggatgac gatgtgcgca ttattcgtcg tatcaagcgt gatatggagg
     5641 agcgtggccg tagccttgat agcgttatta accagtactt aggtgtggtc aaaccaatgt
     5701 accaccagtt tatcgagtca actaagcgtt atgctgatat cgtcattcct gaaggggtta
     5761 gcaataccgt ggctatcgac ctgttgacga ccaagattgc aaagattttg gaagaagctc
     5821 gaaacagcaa ataatcacat gtggaggcct tgcctccttt ttctattttt ccctcataat
     5881 ggtacataaa tggagatttt taggcatatt tttggtataa taatacccat gaaagagcaa
     5941 gtaaatgaat agtaggtgga gatggaaaag tatttatcgg taacaacttt gaccaagtat
     6001 ctgaaaatga aattcgataa agacccatac ttggaacggg tctatttaac tggtcaagtt
     6061 tccaactttc gtaaacgacc tactcaccaa tatttctccc taaaggatga ccatgcagtt
     6121 attcaagcga ccatctggtc tgggatttat cagaaattag ggtttgacct ggaagaagga
     6181 atgaagatca atgtgattgg gcgtgtacag gtctatgaac caagtggtag ctactccatc
     6241 atcattgaaa aagctgagcc tgatggggtt ggggcgcttg cgattcagtt tgaacaactt
     6301 aagaaaaaat tgacagaaga agacctgttt caagaacgct tcaagcaagc tctgccccaa
     6361 ttttctaaga gaattggtgt agtaaccagc cgtagtggag ccgttattcg agatattatt
     6421 acgaccgtca gcaggcgatt cccaggtgtt gacatccttc tttatccgac caaggttcaa
     6481 ggtgaagggg ctgcggagga aattgctaga aatattgcgc gtgctaatca acgggacgat
     6541 ttggatttgc tcattattgg tcgtggaggt ggttctattg aggatctctg ggcctttaac
     6601 gaagaaattg tggtacgagc tatttttgaa tctcgtttac cagttatctc tagtgtgggg
     6661 catgagacgg atgtgacctt ggcagatttt gtggcagatc gacgcgctgc aacgccaaca
     6721 gcggcggctg aactggcaac acctgtgacc aagttggatg tattagctca tttgcaaaat
     6781 caggaaaaac ggatggcaac ggcagtccga aatgttctat ctaagaaaca agaggctttg
     6841 aaaaaatgca gtcagtctgt tatctttaga caacctgagc gcttgtatga cggttatttg
     6901 caacgcttgg accaactgca actgcgtttg aaacaaagtt tgcgaactcg gatttctgat
     6961 aacaaacaat tagttcaagc aagaactcat caattagtac aattatcacc tgttaccaaa
     7021 atccaacgct atcaagaccg tttaggacag ttggacaagc tcttaggtag ccaaatggcg
     7081 ttagtttatg acgccaaggt tgctgaggcc aagcgacttt cggaagcttt gctcatgttg
     7141 gatactagcc gaatcgtggc gcgtggttat gctattgtca aaaaagaaga gtccgttgta
     7201 gattcggttg agagtttgaa gaaaaaagac caagtaacgc ttttgatgcg agatggtcaa
     7261 gtagaattag aggttaaaga tgtcaaaaca aaagaaattt gaggaaaatc tagcagaact
     7321 ggaaaccatt gtccaaagtt tggaaaatgg tgaaattgct ctggaagatg cgattactgc
     7381 ctttcaaaag ggcatggtct tgtcaaaaga gctccaagct acgctggaca aggctgaaaa
     7441 gaccttggtc aaggtcatgc aagaagacgg aacagaaagt gattttgaat gaaaaagcaa
     7501 gaaaaattag ctcttgtcga gtcggctttg gaagattttt atggagacca gcagtttgcc
     7561 tctagtttac gggagtctgt tctctattct attcatgctg gtggcaagcg tattcggcct
     7621 tttctcttgt tagaagttct ggaagccttg caggttgcca tcaaacctgc tcacgcgcag
     7681 gtagctactg ccttggagat gattcataca gggagcttga ttcacgatga ccttcctgct
     7741 atggatgatg acgattatcg aagagggcgg ttaaccaatc acaagaaatt cggtgaagct
     7801 atggccattt tggctggaga tgccttattc ttagacccat atgccttgat tgcgcaggca
     7861 gatttgccaa gtcagatcaa ggtggacttg attgccaact tatcccttgc ttcaggtagt
     7921 ctgggtatgg tggcagggca agttttggat atggagggcg aacaccagca cttgtctctg
     7981 gaagaacttc agactattca tgccaataag actgggaagt tactagccta tcccttccaa
     8041 gcggcagcta ttatagctga attgtcacct gaaatgcagg tgaagctgaa aactgtgggt
     8101 gaattgattg gacttgcttt tcaagtcaga gatgatgtac tggatgtgac agctagtttt
     8161 gaggaaatcg gcaagacacc tcaaaaggat ctgcaggcag aaaaatcaac ctatcctgcc
     8221 ttgttgggct tggaagagtc cattgccttt tgtaaccaaa ccctggatca agctaatgaa
     8281 aaattagaag aaattgccca gcagattccc tttgaaacag aatcgattgt aagtgtagta
     8341 gaaagtttga gaatcaatgg ctaaggaaag agtggatgta ctagcttata aacaggggtt
     8401 gtttgaaacg agagagcagg ccaagcgagg tgtgatggct ggcctagtcg tagcagtcct
     8461 taatggagaa cggtttgaca agccaggaga gaaaattcca gatgacaccg aattaaaact
     8521 caagggggag aaactcaagt atgtcagccg tggtggtttg aaactggaaa aggccttgca
     8581 ggtctttgat ttgtcggtgg atggcgcgac tacgattgat atcggggcct ctactggagg
     8641 ttttaccgat gtcatgctac agaatagtgc caagttggtc tttgcagtcg atgttggtac
     8701 caatcagttg gcttggaaat tacgccaaga cccacgagtt gtcagcatgg agcagttcaa
     8761 tttccgctat gctgaaaaga ctgatttcga gcaggagccg agctttgcca gtattgatgt
     8821 gagtttcatt tcccttagtc tgattttgcc agccttgcac cgtgtcttgg ctgatcaagg
     8881 tcaggtggta gcacttgtca aacctcagtt tgaggcagga cgtgagcaga ttgggaaaaa
     8941 tggaattatt cgagatgcta aggttcatca gaatgtcctt gaatctgtaa cagctatggc
     9001 agtagaggta ggtttttcag tccttggctt ggacttttct cccatccaag gtggacatgg
     9061 aaatattgaa tttttagcgt atttgaaaaa agaaaagtca gcaagcaatc agattcttgc
     9121 tgagattaaa gaagcagtag agagggcgca tagtcaattt aaaaatgaat aaaaaagaga
     9181 gacttgaaaa aattagaaga ttggtgacag attatcaaat cggcacgcaa gaagaaattg
     9241 tagaacattt gaaagaagca ggtatcactg ccactcaggc gacggtatcc cgagatatca
     9301 aagagttagg tattgtcaaa attcctttga gagacaacac ctatgtctac gaattaccaa
     9361 aatcaatcgt aaaaagtctg caactggctg aagacaatat cgaatcggct gaattgatgg
     9421 ataagatgat caatctccaa gttattccag gaaatacggc ttttgtaaaa gctcagttaa
     9481 tcgagacttt tgcagacaag atttttagct gtttgactga tgatagctcg attttagtca
     9541 ttgccagaag tggaagtcta gcagaggaaa tctttgaaca agtaaaaaat tggtaggtct
     9601 atatgttact tgaaatttcg ataaaaaact ttgccattat tgaggctatt tccctcaatt
     9661 ttgaaaaggg gatgactgtc ctgactggtg aaacgggtgc agggaagtcg attatcattg
     9721 atgccatgaa tatgatgttg ggagctcgtg cgacgacaga tgttattcgt catggtgcac
     9781 caaaggcaga gattgagggg cttttctcag ttgagaatag tcgtctttta caggaaattt
     9841 ttgataagca aggtttagaa ttaggtgatg aaattatcat ccgtcgggaa atcttgcaaa
     9901 atggtcgtag tatcagccgt gtaaatggtc agatggttaa tctgtctgtt ttgcgagcta
     9961 ttgggcaaca tcttgtagat attcatggtc agcatgacca tgaggagtta atgcgtcccc
    10021 aactgcatat ccagatgttg gatgaatttg gtgatgccgc tttttgggac ttgaaagaaa
    10081 cctatcaaac gagttttgat gcctatcgga aaatgcgcaa gcaggttctg gaagtcaaga
    10141 aaaaccaaca ggaacacaag gcacgtatcg aaatgttgga atttcaaatg gcagagattg
    10201 aggcagcaaa cttgcaggct ggagaagact tggctctcaa tcaagagcga gataaactcc
    10261 tcaaccacaa aaatattgcg gatacactga ccaatgccta cagtatgttg gacaatgaag
    10321 atttttcaag tctggccaat gttcgttcag ctatgaatga catggaaagt gtcgaagagt
    10381 atgatcctga ataccgtgaa atttcaagct atctgtctga gacctactat gttttagaag
    10441 acattagcaa acgtttggaa gctatcattg aggaccttga ttttgatggc aatcgcctga
    10501 tgcaggttga gaatcgtttg gacctccttc atactattac tcgtaagtat ggtgggactg
    10561 ttgatgatgt tttgctttat tttgccaaga ttacggaaga atacaatctc ttgacaggca
    10621 ataatctttc gtctgaggac atggaagcag agcttaagaa gttggaagtc aatcttgtca
    10681 atttggcagg tcaacttgct tctgctcgtc ataatttggc tcagcaactc gaagctgaga
    10741 ttaaacaaga actgcaagat ctttatatgg aaaaagccca gtttcaggtt cgttttagta
    10801 agggaaaatt cagtcgtgag ggaaataaaa tggttgagtt ttatatttca accaaccctg
    10861 gagaagactt taaacccttg gttaaggttg cttctggagg ggaattatct cgtctcatgt
    10921 tagccattaa gtctgccttt tcacgtaaag aaggcaagac tagcattgtc tttgatgagg
    10981 tggatacggg agtttcaggt cgtgttgctc aagctattgc tcagaaaatt cataaaattg
    11041 gtcagcatgg tcaggttttg gctatctccc atttgccaca agtaattgcg attgcagatt
    11101 atcaattctt tattgagaag attagtaatg accattcaac ggtttcgact gttcgtctct
    11161 tgacggtcga agagcgagtg gaggaagttg ccaagatgtt ggcaggtgat gatgtgacag
    11221 aagcagccct gacgcaagca agagaattgt tgagaaacag ggagaaataa gatgacagat
    11281 tattatgtaa ttggagatgt tcacggaaaa gctgggatgc tggaagacct tctcaaaacc
    11341 tgggatggtc agccccagtt gctctttcta ggtgatttga ttgacagagg tgaggatagt
    11401 caccgtgttc ttgaaatggt caaggacttg gtggacaatc aaggggctat ctgtttgtcc
    11461 ggaaaccacg agtatatgtt tttgacttgg ctagatgacc cagaagaaag ttatgaccat
    11521 tatagtcgca atggtggaga tacaaccatt aactctatcc taggtcgtcc cttggatgca
    11581 ccagttgatg gagtagaaga tgccaagcgg gttgccgctg aagcgtcaga cttggtcgaa
    11641 ttcattcgtc aaatgccatt tgtagtagag acagacaagt atatatttgt tcacgcaggt
    11701 attgatttga ctttggacga ctggcatgaa acaacagatt ataaaaaagt atggcttaga
    11761 aaaccattcc acggagctga aaatcatact ggaaaaacca ttgtcttcgg tcatacaccg
    11821 gtctatggtt tgttacagca agaacgcggt acatctgagc tttggactac agaagatggc
    11881 aagattggaa tggatggagg agctgtttat ggtggtgtcc ttcacgggat tgtctttaca
    11941 gaccaaggaa tgacagaaca ccactttatc gaaaatgacg gctttgtcgc tgaagattag
    12001 tactcctagc agggtatggt cttgtcaaaa tatcaaaaac aatttataat aaatagatac
    12061 cctgaaagga agagaatcat gaacttagaa gaattgaaaa aacgacaggg gaagatccgt
    12121 aacttctcta ttatcgccca tattgaccac gggaaatcaa ctctagcaga ccgcattttg
    12181 gaaaaaacag agacggtttc aagtcgtgaa atgcaggccc agcttttgga tagcatggat
    12241 ctagagcggg aacgtggaat tactattaag ttgaatgcca tcgagttgaa ttacactgca
    12301 aaagatgggg aaacttatat tttccacttg attgacacac cagggcacgt tgactttacc
    12361 tatgaagttt cacgttcgct agctgcctgt gagggtgcta ttttggtggt cgatgcggct
    12421 caaggaattg aggctcaaac tcttgccaac gtttatctgg ctttggataa tgatttggaa
    12481 atcatgccta tcattaataa aattgacctg ccggctgcag atccggagcg cgtgcgtaca
    12541 gagattgaag atgtgattgg tttggatgcc agtgaagcag ttttggcttc tgccaaggct
    12601 ggtattggga ttgaagaaat cctcgagcaa attgtagaaa aagtaccagc accaacgggt
    12661 gatgtgacgg cgccacttaa ggccttgatt ttcgactctg tttacgatgc ttaccgtggg
    12721 gttatcctcc aagtgcgtgt catggacgga gtggtcaaac ctggtgataa gattcagctc
    12781 atgagcaata gtaagacctt tgatgtggcc gaagtcggta tttttacacc aaaagcggtt
    12841 ggtcgtgatt tccttgcgac tggtgatgtt ggttacattg cggcgtctat caagacggtt
    12901 caggatactc gtgtgggtga taccgttacc ttggcaacca atcctgcggc agaaccatta
    12961 catggttata agcagatgaa tcctatggtc tttgcgggtc tctacccaat cgaatcaaac
    13021 aagtacaatg acctgcgtga agcgcttgaa aaattgcaac tgaatgatgc tagtcttcag
    13081 tttgaaccag aaacatctca ggcacttgga tttggtttcc gttgtggatt tcttggactt
    13141 ctccatatgg atgttattca ggaacgttta gagcgtgagt tcaatattga cctcatcatg
    13201 acagctccgt ctgttattta caaagttaat ttgaccgacg gtgagtctat ggatgtgtct
    13261 aacccatctg agtttccaga cccaactaag attgcgacca ttgaagagcc ttatgtcaag
    13321 gcgcaaatca tggtaccaca ggagttcgtc ggagcagtaa tggagctagc tcagcgtaag
    13381 cgtggggact ttgtgactat ggactatatt gatgataacc gtgtcaatgt tatctatcaa
    13441 attcctcttg ctgaaattgt ctttgacttc tttgataaac ttaagtcttc gacacgtggt
    13501 tatgcaagct ttgactacga attgtcagaa tatcgcccat ctaagctggt gaaaatggat
    13561 atccttctca atggagataa ggtggatgcc ctcagcttta tcgttcacaa ggactttgcc
    13621 tacgaacgtg ggaaactcat cgttgataaa ctcaagaaaa tcatccctcg ccaacaattt
    13681 gaagttccaa tccaagcggc tattggacac aagattgtcg ctcgtactga tatcaaggcc
    13741 cttcgtaaga acgtgcttgc taaatgttat ggtggtgacg tttctcgtaa gcgtaaactc
    13801 cttgaaaaac aaaaagctgg taagaaacgc atgaaatcca tcggatcagt tgaagttccg
    13861 caagaagcct tcctcagcgt cttgagtatg gatgaagaat aagttatcag aaatgccttt
    13921 ccttgttcac aggaaattta tataaaaatc aatagtaaag caggcttaga tggtctgctt
    13981 ttttagtttt tatactcttc gaaaatctct tcaaaccacg tcagcgtcgc cttaccgtat
    14041 gtatggttac tgacttcgtc agtttcatct acaacctcag aaccatgttt tgagctgact
    14101 tcgtcagttt catctacaac ctcaaaatca tgttttgagc tgacttcgtc agtttcatct
    14161 acaacctcaa aatcatgttt tgagctgact tcgtcagtct tatctacaac ctcaaaactg
    14221 tgttttgagt aacctgcggc tagcttccta gtttgctctt tgattttcat tgagtattag
    14281 aacagaaaaa tgcttggagt atttgtttgt gtgtttattt ttatataaca aactataaac
    14341 aaaacaaaaa tataaaaaaa gagacaaaaa agaatagaaa gtaattaaca acgatttcat
    14401 atagtgttat acttatagca taaagttaat ggaaagaagg gaaaacctta tggtattaga
    14461 ttatttcttt gacaaaaacc ttgtgttttg cttagaagcg gataatcaag aacaactctt
    14521 tgatcaggta gcaagcttgt tggaagaacg agaaattgtc actccaactt atcgtgaagc
    14581 cttgatcacg cgtgaaaagt catttccaac tggtttagat atggaatttc taggaaagga
    14641 cttgccaaat gtagcgattc ctcacacaga tattgttcat aatctagctg aaaaagtggt
    14701 ggttgttcga ttagaaaaac cagtaacttt tcacaatatg atagctcctg ataaggaagt
    14761 agaagtatcc ctactcttct ttatcattaa caattcaagt tcaagtcaaa caaatattct
    14821 ggctcagttg atggactttt tcacaggaaa tggacatctt gaagatttat caaaaatttc
    14881 tgaaccagaa gccctttatg cttacattgc tgaagcaacc gcttaatctt gtctattaaa
    14941 aaataaaatc ggaggaaatc caaatgatta aaattcttgc tgcctgcggt gaggtgttaa
    15001 ctcgagttac caaattaaaa gtgctctaga agaagaactt tcaaaccgtg gttatgatgt
    15061 tcactgtgat gcagtcatgg tgaaagatgt aaacgaagac cttatgaaag gttacgatat
    15121 ctttacacca atcgctgcaa cagatcttgg ttttgaacca ggtatcccag ttatcgaagc
    15181 tgggccaatc ttattccgta taccagcaat gagcgctcca gtatttgaca atattagact
    15241 ccctgcgaaa caaaacatgg tatagtagtt ctatgaatga tgaagcaagt aaacaactaa
    15301 ctgatgcacg atttaagcgt tttgttggtg ttcagcgcac gacttttgaa gagatgttag
    15361 ctgtattaaa aacagcttat caacttaaac acgcaaaagg tggacgaaaa cctaaattaa
    15421 gcctagaaga ccttcttatg cccactcttc aatagtgcga gaatatcgaa cttatgaaga
    15481 aattgcggct gattttggta ttcacgaaag caactttatc cgtcggagcc aatgggttga
    15541 aataactctt gttcaaagtg gttttacggt ttcaagaact cctctcagtt ctgaggacac
    15601 ggtaatgatt gatgcgacgg aagtaaaaat caatcgccct aaaaaaacaa ttagcgaatg
    15661 attctggtaa aaagaaattt cacgctatga aggctcaagc gattgtcaca agtcaaggga
    15721 gaattgtttc tttggatatc gctgtgaact atagtcatga tatgaagttg ttcaaaatga
    15781 gtcgtagaaa tatcgaacaa gctggtaaaa tcttggctga cagtggttat caagggctca
    15841 tgaagatata tcctcaagca caaactccac gtaaatccag caaactcaag ccgctaacag
    15901 ctgaagataa agcctataac catgcgctat ctaaggaaag aagcaaggtt gagaacatct
    15961 ttgccaaagt aaaaacgttt aaaatatttt caacaaccta tcgaaatcat cgtaaacgct
    16021 tcggattacg aatgaatttg agtgctggta ttatcaatca tgaactagga ttctagtttt
    16081 gcaggaagtc tattgaggta ttgagctagt ttatgaaaaa attgggtgaa aagtcgagtg
    16141 ttttagaaac ccacagtgta gtattctagt ttcaatccac tatattttgc tactccccgt
    16201 aaagtttcta ttttccctga tttctgatat aatagaaata ttgacttcaa gagtaaggaa
    16261 gagaagatga acgcattatt aaatggaatg aatgaccgtc aggctgaggc ggtgcaaacg
    16321 acagaaggtc ccttgctaat catggcaggg gctggttctg gaaagactcg tgttttgacc
    16381 caccgtatcg cttatttgat tgatgaaaag ctggtcaatc cttggaatat cttggccatt
    16441 acctttacca acaaggctgc gcgtgagatg aaagagcgtg cttatagcct caatccagcg
    16501 actcaggact gtctgattgc gaccttccac tccatgtgtg tgcgtatttt gcgtcgcgat
    16561 gcggaccata ttggctacaa tcgtaatttt acaattgtgg atcctggtga acagcgaacg
    16621 ctcatgaaac gtattctcaa acagttgaac ttggacccta aaaaatggaa tgaacgaact
    16681 attttgggga ccatttccaa tgctaagaat gatttgattg atgatgttgc ttatgctgcc
    16741 caagctggcg atatgtatac gcaaattgtg gcccagtgtt atacagccta tcaaaaagaa
    16801 cttcgtcagt ctgaatccgt tgactttgat gatttgatta tgctgacctt gcgtctcttt
    16861 gatcaaaatc ctgatgtttt gacctactac cagcaaaaat tccaatacat ccacgttgat
    16921 gagtaccaag ataccaacca cgctcagtac caattggtca aactcttggc ttcccgtttt
    16981 aaaaatatct gtgtggttgg ggatgcggac cagtctatct acggttggcg tggtgctgat
    17041 atgcagaata tcttggactt tgaaaaggat taccccaaag ccaaggttgt tttgttggag
    17101 gaaaattacc gctcaaccaa aaccattctc caagcggcca acgaggttat taaaaataat
    17161 aaaaatcgcc gtcctaaaaa tctctggact caaaacgctg atggggagca aatcgtttac
    17221 tatcgtgccg atgatgagct ggatgaggct gtatttgtag ccagaaccat cgatgaactt
    17281 agtcgcagtc aaaacttcct tcataaggat tttgcagttc tctatcggac taatgcccag
    17341 tcccgtacaa ttgaggaagc cctgctcaag tctaacattc cttataccat ggttggcgga
    17401 accaaattct acagccgtaa ggaaattcgc gatattattg cttatctcaa ccttattgct
    17461 aatttgagtg acaatattag ttttgagcgt attatcaacg agcctaaacg tggaattggt
    17521 ctaggtacag ttgagaaaat ccgtgatttt gcaaatttgc aaaatatgtc tatgctggat
    17581 gcttctgcta atattatgtt gtctggtatc aagggtaagg cagcccaatc tatctgggat
    17641 tttgccaata tgatgcttga tttgcgggag cagctagacc acttaagcat tacagagttg
    17701 gttgagtccg tcctagaaaa aacaggttat gtcgatattc ttaacgccca agcgactcta
    17761 gaaagcaagg cacgggttga aaatatcgaa gagtttcttt ctgttacgaa gaactttgat
    17821 gacaccacgg atgtgacaga agaggaaact ggtctggaca aactgagtcg tttcttaaat
    17881 gacttggctt tgattgccga cacagattca ggtagtcagg agacatcaga agtgaccttg
    17941 atgaccctgc atgctgccaa aggtctcgaa tttccagttg tctttttgat tgggatggaa
    18001 gaaaatgtct ttccacttag tcgtgcgact gaagattcag atgaattaga agaagagcgc
    18061 cgtctagcct atgtaggtat cacgcgtgca gagaaaattc tctatctgac caatgccaac
    18121 tcacgcttgc tttttggtcg taccaattat aaccgtccga ctcgttttat taacgaaatc
    18181 agttcagact tgcttgagta tcaaggtctg gctcgtcctg caaatacaag ctttaaggca
    18241 tcatatagca gtggtagtat ttcctttggt caaggtatga gtttggctca ggctcttcaa
    18301 gaccgtaaac gcggtgctgc cccaaaatca atccagtcaa gcggtcttcc atttggtcaa
    18361 tttacagctg gcgcaaaacc agcatctagc gaggcaaatt ggtccattgg tgatattgct
    18421 ctccacaaga aatggggaga gggaaccgtt ctggaagttt caggtagcgg tgctaggcag
    18481 gaattgaaaa tcaatttccc agaagtaggt ttgaaaaaac ttttagccag tgtggctcca
    18541 attgagaaaa aaatctaatt ttccatcctt ctcacgaata ataaagtgag gaggattttt
    18601 atgtacagta tttcattcca agaagattca ctattaccaa gagaaaggct ggccaaggaa
    18661 ggagttgaag cgcttagtaa ccaagagttg ctagctattt tactcaggac aggaacacgt
    18721 caagctagcg tttttgaaat tgcccaaaaa gtcttgaaca atctttcaag cctaacggat
    18781 ttgaaaaaaa tgaccctgca ggaattgcag agtttgtctg gtattgggcg tgttaaggcc
    18841 atagaattac aagctatgat tgaactgggg catcgtattc acaaacacga gactcttgaa
    18901 atggaaagta ttctcagcag tcaaaagttg gccaagaaga tgcagcagga attaggggat
    18961 aaaaaacaag agcacctggt ggcactctat ctcaatactc aaaatcaaat catccatcag
    19021 cagaccattt ttatcgggtc tgtaactcgt agtatcgctg aaccgcgaga gattcttcac
    19081 tatgcaatca agcatatggc gacttctctt atcttggtcc acaatcatcc ttcaggagcg
    19141 gtagcgccta gccaaaatga tgatcatgtc actaaacttg ttaaagaagc ctgcgaattg
    19201 atggggattg ttctcttgga ccatttgatt gtctctcatt ctaattactt tagttatcgt
    19261 gaaaagacag atttaatcta aagttcatta acgacatagt caaagagttt tttatctttg
    19321 ggacgatttt caaaaagaag ttctggatgc cattggacac cgagaaaggc gacatcatcc
    19381 gtactcatga cagcctcaat gataccatct ttaggatcat gagccacaac ttttaaattt
    19441 ggtgctaagt ccttgatgct ctggtggtgg aaggagttga tatgagagat ttctccatag
    19501 atttcttgga gaacggtatc tggttctgtt accaagcgtt gagttgtgta ctcaacagaa
    19561 gaatcctgcc aatggtcttc gatatcttgg tacaaagttc cacccatggc aacgttaaag
    19621 agttgggtac cacggcagac agagaaaatg ggctttttct gtttaatagc ttccttgatg
    19681 agggccagtt cgaagatatc tctttgaagg tgatagtcat cactatcaat ggttttgggt
    19741 tcgccataaa attttggatc gacattttgc ccacctgtca agatgagctt gtcaatcaaa
    19801 ctgatatagt ggcaggccat ttcttgatca ccaatcggta ggatgatggg aatccctcca
    19861 gcatctttaa cgccttcaac aaagcctttt gctgcgtagc tcatcatgat gtcatcatct
    19921 ggatgagttt tttcgtttcc tgtaatccca ataactggtt ttttcataaa atgattttcg
    19981 ctttctaatc ctcttttcgc atgaagtaga ggagggtttg gagttcactt gtcaaatcga
    20041 catactgaac gaccacgtct tttggtaaat gcagatggac tggtgaaaaa ctgagaattc
    20101 ctttcacacc agcatcaacc aagagattag caacctcttg tgacttgacg ctgggaacag
    20161 ttaggatagc agtcttcaca tcagcatcct tgattttatc cttgatctga gaaatcccgt
    20221 aaatgggaat cccgtcagga gtttgggtac cgacttcagg atggtcgtct aggtcaaagg
    20281 ccatgataat cttcatcttg ttacgttcgt ggaagcggta gtggagaagg gcatggccca
    20341 tatttccaat accaaccagc atgacattgg taatagagtt gtcattgagc aaatcggcaa
    20401 aaaatgtcat tagttttttg acatcatagc caaaaccacg acgaccaagt tcaccaaaat
    20461 aggaaaaatc acgacgtacg gtcgctgaat caataccgat agcctctgca atttgcttag
    20521 agttggcacg ttcaatcttt tctgcatgaa atctcttaaa aattcgatag tagagagaga
    20581 gtctttttgc tgtagctttt ggaatagcaa actgtttatc tttcacaaaa tcacaacctt
    20641 tctattcttc tattttatag aaacattgtg aaaaaatcaa caaaaataag aaaaaactaa
    20701 gaaaaatctt agttttgatg taaaaaatct gcatgagata gaaaacggta gaggtctccg
    20761 accagcccct gataaacttt tttgccccta aaagtcagag aagtcacata aagtgtatct
    20821 ggtaaggtta cacatcctga caaagtcaac atgagagcct catgatcctc atacttgaga
    20881 gtacgctcta catgatagca gtccttatag gtcagttcaa acattttggc tctatctttc
    20941 cgattttgta aagacaccac gttctaccaa gctatccatg aggaagtaga atttttcctg
    21001 atgaatatgg tggtcttctg atttgaaaat atcaactaga cgaaggccaa acttgtcagt
    21061 gatattgatt ttagcccctg taagttcctt gttaatgatg attttgagtt ggaagccttc
    21121 accgctgttt ggcacttttt ccaaaaggcg agtcagttca tagttaccaa ccttagtttc
    21181 aaaaaaggtg ttatctttga gggtgaattt tttaacagaa gggctaagag tgtaatcgta
    21241 acgacaattt tttaactgaa tgattttttc aaatgccata tggctaacct ccgataattt
    21301 cttttaaggt ttttgcgagg gtttgtaggt cttcaacggt attttgtggc gacaaactga
    21361 tgcgaaggga ttccttcaag cgttctgaat ttgcgccata catggcttta agaacatggc
    21421 tggattggac aacgcctgca gtacaggctg agccagtaga gattgaaatt ccagctaaat
    21481 ctagccgaag gagtaagagg tcatttttct gaccaggaaa tccaatattg agaacataag
    21541 ggagatgatg ttttcctcta ttcaagtaat actgaatgcc ctccagctct gccagaaagg
    21601 cagtttctag attttgtaca tgttgaaaat gttcttcttg tttttctagg tcttctttta
    21661 gggctgcaac catgcctacg atggcaggca gattttcagt tcctgcacgt tttttctgtt
    21721 cctgatctcc gccatgtaga taggaatcaa agtccatgct agatgcgtag agaaaaccga
    21781 ttcccttagg accatggaat ttgtgggcag aagcagtgag aaaatcaatg cccaattctt
    21841 ctgaatgaat tgggatttta ccaatagcct gaactgcatc aacatgatag gcagcagggt
    21901 gttgcttgag tatttggcca atttcagcga tgggaagtag gtttcctgtc tcattattgg
    21961 caaacatagt agaaaccaaa atcgtatcgt cacgtaaagc cttttgaatt tgctgggctg
    22021 tgatttcttg attttctggc tggataatgg ttgcttcaaa cccaaagtgt tgaaccaagt
    22081 aatcaattgt ttcaaggaca gcatggtgct cgatggcagt tgtgatgata tgttttcctt
    22141 gttcttggtg acgaagacag tagccaatga tggtagtatt attgccttca gtcccaccag
    22201 aagtgaaaaa gatatgttga ggttttgtcc ttagtaactg ggctagttcc tgacgggctt
    22261 ctcgcaagag tttgccagct tgacgaccat gaccatgaat actagaagga tttccgtggg
    22321 tttcttgcat aaccttggtc atagctgaaa tagcaactgc tgacatagga gtcgttgcag
    22381 cattgtccaa ataaatcaaa gaatcacctt atttcttttt attgtaggca aagagtgggc
    22441 tgactggttt tctttcgtga atacggacga tagcatcacc aattaactca ctagcagtga
    22501 tgtagcatac atttttagga gttttttctt ttgttgctac tgaatcagtc acaagaattt
    22561 ctttaatatt agtattgtca agaagctcag cagctccctc gacgaagaga ccgtggctag
    22621 aaacagcata aatttctgta gctccttcac gttcaacgat tttagaagct tcagagaagg
    22681 tacgtcctgt atttaaaata tcatcaatca agatagcttt cttaccttca acatcaccaa
    22741 taatataacc ttcgttacga gttgcatcgt cttgagggta gtcgataatg gcgataggag
    22801 catcaagata ttcagccagg ctacgcgcac gtttgacacc tgaattttta gggctaacga
    22861 caacaacatc tgaaccaagc aatcctttat cgcagtaatg ttttgcgaat aggggaacag
    22921 tgaaaagatt atccactgga atatcaaaga aaccttgaac ctgaacggca tgcaaatcaa
    22981 gagtcaggat acgatcaact ccagccttaa ccagcatatt ggcaactagt tttgctgtaa
    23041 gtggctcacg aggacaagca atgcggtctt gacgtgcata gccaaaatat ggaaggacaa
    23101 cgttgatact gtgggcactt gcacgcacac aagcatcgac catgattaac aattccatta
    23161 ggtggttgtt gacagggaaa cttgttgatt ggatgatgta aacatcataa ccacggacac
    23221 tttcttcgat atttacttgg atttctccgt ctgaaaattg acgtgatgat agttttccaa
    23281 gtgggacacc aacagcttgt gcaatttttt gtgcaatctc ttggttagag ttgagtgcga
    23341 aaagtttcat gttttttcta tctgacatta tagaccgtcc tctgtaaact ttataaatcc
    23401 tagttatatt taccttacat atatgaactg ggatttgtgt atttttatct tttctatttt
    23461 accaaaaaat ggagattatt tcagctattt ttcatacttt tgacaaatcg aaccaatttt
    23521 gaaggagctt tttggtagga aatctgattt ttctctaaaa attgtcggaa atcctgtttg
    23581 ccttgctcat gattttccac ttcaagctcc aattcgtaat ctgttatatc aaagtatcgg
    23641 ctctgatcca gtgccatgag accaatagct gttttcattt catagcgaag cgttgttaga
    23701 caaccaagaa cctgccagtt cttactttgg ataccatgtt tcgccaattc atccagtact
    23761 agcccttgag gaagttcttc cttactcaga tagttctcag catcttttag ttgcaatttt
    23821 tggttgtatt ccatgtttcc aacactctgc gggactttga gtgtcaactc agcccagtct
    23881 tcaaaggttc gaatgcgcat agcgactttc ttttctcgca gttcaaaatc aggcgtgtcg
    23941 atgtagtaat ttgtttgaag aacaggagtg acacctgtga actggtcttt tagacgattg
    24001 tattcatctt ttttcaatag tgttttcaat tcaatttcta aatgtttcat ttttcttacc
    24061 tttttttatc gttgaaagcg gatttatggt ataataagca ttgtatttat tgtatatgaa
    24121 tctggagaaa aaatcaaaga tatttttgac ggataatatg agaacaaggg agaatatatg
    24181 accttagaat gggaagaatt tctagatcct tacattcaag ctgttggtga gttaaagatt
    24241 aaacttcgtg gtattcgtaa gcaatatcgt aagcaaaata agcattctcc aattgagttt
    24301 gtgaccggtc gagttaagcc aattgagagc atcaaagaaa aaatggctcg tcgtggcatt
    24361 acttatgcga ccttggaaca cgatttgcag gatattgctg gcttacgtgt gatggttcag
    24421 tttgtagatg acgtcaagga agtagtggat attttgcaca agcgtcagga tatgcgaatc
    24481 atacaggagc gagattacat tactcataga aaagcatcag gctatcgttc ctatcatgtg
    24541 gtagtagaat atacggttga taccatcaat ggagttaaga ctattttggc agaaattcaa
    24601 attcgtactt tggccatgaa tttctgggca acgatagaac attctctcaa ctacaagtac
    24661 caaggggatt tcccagatga gattaagaag cgactggaaa ttacagctag aatcgcccat
    24721 cagttggatg aagaaatggg tgaaattcgt gatgatatcc aagaagctca ggcacttttt
    24781 gatcctttga gtagaaaatt aaatgacggt gtaggaaaca gtgacgatac agatgaagaa
    24841 tacaggtaaa cgaattgatc tgatagccaa tagaaaaccg cagagtcaaa gggttttgta
    24901 tgaattgcga gatcgtttga agagaaatca gtttatactc aatgatacca atccggatat
    24961 tgtcatttcc attggcgggg atggtatgct cttgtcggcc tttcataagt acgaaaatca
    25021 gcttgagaag gtccgcttta tcggtcttca cactggacat ttgggcttct atacagatta
    25081 tcgtgatttt gagttggaca agctagtgac taatttgcag ctagatactg gggcaagggt
    25141 ttcttaccct gttctgaatg tgaaggtctt tcttgaaaat ggtgaagtta agattttcag
    25201 agcactcaac gaagccagca tccgcaggtc tgatcgaacc atggtggcag atattgtaat
    25261 aaatggtgtt ccctttgaac gttttcgtgg agacgggcta acagtttcga caccgactgg
    25321 tagtactgcc tataacaagt ctcttggcgg tgctgtttta caccctacca ttgaagcttt
    25381 gcaattaacg gaaattgcca gccttaataa tcgtgtctat cgaacactgg gctcttccat
    25441 tattgtgcct aagaaggata agattgaact tattccaaca agaaacgatt atcatactat
    25501 ttcggttgac aatagcgttt attctttccg taatattgag cgtattgagt atcaaatcgc
    25561 ccatcataag attcactttg tcgcgactcc tagccatacc agtttctgga accgtgttaa
    25621 ggatgccttt atcggtgagg tggatgaatg aggtttgaat ttatcgcaga tgaacatgtc
    25681 aaggttaaga ccttcttaaa aaagcacgag gtttctaagg gattgctggc caagattaag
    25741 tttcgaggtg gagctattct ggtcaataat caaccgcaaa atgcaacgta tctattggac
    25801 gttggagact acgttaccat tgacattccc gctgagaaag gctttgaaac cttggaggct
    25861 attgagcttc cattagatat tctctatgag gatgaccact ttctagtctt gaataaaccc
    25921 tatggagtgg cttctattcc tagtgttaat cactctaata ccattgccaa ttttatcaag
    25981 ggttactatg tcaagcaaaa ttatgaaaat cagcaggttc acattgttac cagactagat
    26041 agggacactt ctggcttgat gctctttgcc aagcacggtt atgcccatgc acgattagac
    26101 aagcagttgc agaagaaatc tatcgagaaa cgctactttg ctttggttaa gggagatgga
    26161 catttggaac cagaagggga aattattgct ccgattgcgc gtgatgaaga ttccattatt
    26221 accagacgag tggctaaagg cggaaagtat gcccatactt catacaagat tgtagcttct
    26281 tatggaaata ttcacttggt ctatattcac ctgcacactg gtcgaaccca tcaaatccga
    26341 gtccattttt ctcatatcgg ttttcctttg ctgggagatg atttgtatgg tggtagtctg
    26401 gaagatggta ttcaacgtca ggctctgcat tgccattacc tatcctttta tcatccattt
    26461 ttagagcaag acttgcagtt agaaagtccc ttgccggatg atttcagtaa ccttattacc
    26521 cagttatcaa ctaatactct ataaaaactg tctcagagta taattattat cttaaaggag
    26581 aaaactcatg gaagtttttg aaagtctcaa agccaacctt gttggtaaaa atgctcgtat
    26641 cgttctccct gaaggggaag agcctcgtat tcttcaagca acaaaacgct tagtaaaaga
    26701 aacagaagtg attcctgttt tgcttggaaa tcctgaaaaa attaaaattt atcttgaaat
    26761 tgaaggaatc atggatggtt atgaggtcat cgaccctcaa cattatcctc aatttgaaga
    26821 aatggtttct gccttggtgg agcgtcgcaa gggcaaaatg actgaagaag atgtacgcaa
    26881 ggttttggtt gaagatgtca actactttgg tgtgatgttg gtttacttgg gcttggttga
    26941 tggaatggtg tcaggagcga ttcactcaac agcttcaaca gttcgcccag ctctacaaat
    27001 catcaaaact cgtccaaatg taactcgtac ttcaggagcc ttcctcatgg ttcgtggtac
    27061 ggaacgttac ctatttggag actgtgccat taatatcaat ccagatgcag aagccttggc
    27121 tgaaattgcc atcaactcag caatcacagc taagatgttt ggcatcgaac ctaaaattgc
    27181 catgttgagc tattctacta aaggttcagg gtttggtgaa agcgttgata aggtcgttga
    27241 agcaactaaa attgctcacg acttgcgtcc tgaccttgaa atcgatggtg agttgcaatt
    27301 tgatgcggcc tttgttcccg aaactgcagc tctgaaagct ccgggaagta cagtagctgg
    27361 tcaagcaaat gtcttcatct tcccaggtat cgaggcagga aatatcggtt acaagatggc
    27421 tgaacgcctg ggtggctttg cggctgtagg acctgttttg caaggtttaa acaagccagt
    27481 taatgatctt tctcgtggat gtaatgcaga tgatgtttac aagttgaccc tcatcacagc
    27541 agctcaagca gttcatcaat agtgaaaact ataaagtgat atactatgct atactgtagt
    27601 tatgaaacta tgtacgaaaa gcactgccat taattcctga gaactaaatt actgattggt
    27661 gtcaaaaagg aaaacttcca agcgatgata tcctgtctat acacgaccta tagaaatctg
    27721 taatatacat atccgtaaaa cgataaattc cctttttgat tttaaatgag tatgaaaaga
    27781 gaattttttg gctctttgtc aactgtagtg ggttgaagtc agctaagctc gagaaaggac
    27841 aaattt
//
LOCUS       DBERDK010000021        26734 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.021, whole genome shotgun sequence.
ACCESSION   DBERDK010000021 DBERDK010000000
VERSION     DBERDK010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 26734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26734
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.021"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(64..1212)
                     /locus_tag="ACL73L_000904"
     CDS             complement(64..1212)
                     /locus_tag="ACL73L_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000876726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGS0771903.1"
                     /translation="MKYNKTKYPNIYYYETAKGKRYYVRRSFFFRGKKREKSKSGFTT
                     LPQARAALVELEQQIQEQELGINTNLTLDQYWDIYSEKRLSTGRWNDTSYYLNDNLYK
                     NHIKTKFGSIQLKNLDRNEYELFIAEKLQNHTRYTVQTLNSSFMALLNDAVKNGNLLS
                     NRLKGVFIGQSDIPAANKKVTLKEFKTWIAKAEEIMPKQFYALTYLTIFGLRRGEVFG
                     LRPMDITQNDSGRAILHLRDSRSNQTLKGKGGLKTKDSERYVCLDDIGTDLIYYLIAE
                     ASKIKRKLGIIKEQHKDYITINEKGGLINPNQLNRNFNLVNEATELHVTPHMMRHFFT
                     TQSIIAGVPLEQLSQALGHTKVYMTDRYNQVEDELAEATTDLFLSHIR"
     gene            complement(1583..1990)
                     /locus_tag="ACL73L_000905"
     CDS             complement(1583..1990)
                     /locus_tag="ACL73L_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HGS0771904.1"
                     /translation="MALFGKKQDESLEVELFTEEPNERVFEFKKSKTVVRIDDYFIRI
                     ARKSNVSNVLLHGLDGEKSILLSEITAYQLKEPGSTVGYLQLVYPGSSDTKGGVFDAV
                     KDENTVTFTKDEKASILELKKAIEKALKDKVKK"
     gene            complement(2035..2418)
                     /locus_tag="ACL73L_000906"
     CDS             complement(2035..2418)
                     /locus_tag="ACL73L_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HGS0771905.1"
                     /translation="MTEKEIISHFQVRIVDFDGELIPDELGFYEKETNTAFLSNKLSK
                     KERVKVLLHELGHKDHTRSEYQNARLRCENEADRNMIHHLVKDALESLDDPKEFDYLK
                     FMSYYNLKTVTNEVMVKEEYQTLIG"
     gene            complement(2431..2796)
                     /locus_tag="ACL73L_000907"
     CDS             complement(2431..2796)
                     /locus_tag="ACL73L_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGS0771906.1"
                     /translation="MFLAFDRIKELADKQKISLNILEEKLGYSTNYLYSLKKGNPKSD
                     RLQEIADYFGVSTDYLLGRTENPNLADDTKEYIWQGKVLNVEEMASNVMMFGGRELTD
                     EKKKIIQSIIEGYLKEAGD"
     gene            3083..3229
                     /locus_tag="ACL73L_000908"
     CDS             3083..3229
                     /locus_tag="ACL73L_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771907.1"
                     /translation="MNEEDLKELLELLATDYGRGYLDGVVGGLSMLLKISKEAEKNRK
                     EEYE"
     gene            3273..3647
                     /locus_tag="ACL73L_000909"
     CDS             3273..3647
                     /locus_tag="ACL73L_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771908.1"
                     /translation="MIETLEIKINKKTYFISARADVPVRYKVIFGRNIFDDFSKIKNN
                     KDDTLSLCLLFCMTDKSSEEFCKFFIENNSYITNKHHYEAIRLFEKHFISKRVTYSLE
                     DIVQILTIYLENTKTQIGGKNE"
     gene            3640..3843
                     /locus_tag="ACL73L_000910"
     CDS             3640..3843
                     /locus_tag="ACL73L_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HGS0771909.1"
                     /translation="MSKELKIIKAKIKTRLIELDMTQAELAKQVSVASSVISELLKYG
                     KGSDYVKEKVVDILGIENPWRNH"
     gene            3986..4702
                     /locus_tag="ACL73L_000911"
     CDS             3986..4702
                     /locus_tag="ACL73L_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HGS0771910.1"
                     /translation="MNEIFNFQGQEVRTLTIDDKPWFVGKDVADILGYANSRKAIFDH
                     VDDEDKTDGVTIRDTMGRNQNPIIINESGLYSLILSSKLPQAKEFKRWVTSEVLPAIR
                     KQGGFIREDLDEDAFIALFTGQKKLREQQATMLEDIDYLKSEQPIHPSYAKSLLKKRK
                     ARVVACLGGIDSPAYADKTFAQSVFRQAEIDFKDHFNINRYDLLPKKFADAALAYWMT
                     WEPSTNTKMKIMELNSFDEG"
     gene            4715..4972
                     /locus_tag="ACL73L_000912"
     CDS             4715..4972
                     /locus_tag="ACL73L_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGS0771911.1"
                     /translation="MDNVLLSLSEWIKSIIKDTITRLVEIEKDSDHYPELMDVNTTCD
                     FLGIKYATFSDNYRYLKGFPKELPGKKWSKRAIKEWLSNQI"
     gene            5058..5378
                     /locus_tag="ACL73L_000913"
     CDS             5058..5378
                     /locus_tag="ACL73L_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771912.1"
                     /translation="MFEPPILDQLMGVGALLLGFAGACRHIKLQEQRKEEERREEQEF
                     ASMIIRGYNHAYERGREAERQEIRKNIRRPFKGFTYDNEPPIGLRPEPLALPEPKQSA
                     IRFL"
     gene            5394..5690
                     /locus_tag="ACL73L_000914"
     CDS             5394..5690
                     /locus_tag="ACL73L_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771913.1"
                     /translation="MEELIEWLDNLIMIVKELEGRESTSRHFITIWENDYKNLLLVKE
                     YLTDYEKLAKDYRYVTLKNKLLKIEKMELEGRYIYEDMRMKYRANRRKWGARYV"
     gene            5683..6489
                     /locus_tag="ACL73L_000915"
     CDS             5683..6489
                     /locus_tag="ACL73L_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HGS0771914.1"
                     /translation="MSEIKWIKITTDIFDDEKICLIDALPDPDAILVIWFKILTLAGK
                     HNSNGLLMMTDKVHYTDEMLATIFRRPLNTVRMAIGVFEQFGMIEIIDGIISLPNWEK
                     HQNVDGMEKIKEQTRNRVAKYRKKQKNLALGNVTGNVTVTDGNALEEDKDKNKNRLDK
                     DKNKKRITTTSSGSEENILELFQSEFRRLLSGFEIEEINHLLNENNVDLVKEALKTAI
                     NSGKPNIKYIGGILRNWQMNNVTTVEQVRQSEKKNKDKKEEQEAKDEWGY"
     gene            6629..7399
                     /locus_tag="ACL73L_000916"
     CDS             6629..7399
                     /locus_tag="ACL73L_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGS0771915.1"
                     /translation="MVDNVFEEIALSYRRNTEQQEEFCEKHNIPLIKILRTESVVCRM
                     CESERIHEENQARVNELADAENERERKYYLEKFSLYDEVLKNATLDNFETPTEKEAEK
                     LVFAKRICREWSEGARNNIVLQGEAGTGKSHLAFAMVKALSEYTKEIAIFINVTDLLM
                     KIKADFSQEEFLVNKIASAKFLVLDDLGMEKDSEWSFTILYNILNKRSNTIITTNLTS
                     ADIQKRYGRPFMSRLMKGVDKDHLMVFNDLTNKRKQYF"
     gene            7414..7608
                     /locus_tag="ACL73L_000917"
     CDS             7414..7608
                     /locus_tag="ACL73L_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771916.1"
                     /translation="MFILRHGTREDKPFLRSVVIGVTGLDISCSEEKKAMRFVSRGAA
                     VQVGKALRGSFGNFYPVEVE"
     gene            7608..7835
                     /locus_tag="ACL73L_000918"
     CDS             7608..7835
                     /locus_tag="ACL73L_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771917.1"
                     /translation="MLELYFVYNGHCKFYLGTFDNVDDLIEQMEDHQWVFSAITHPRF
                     QKHIGQRTTRFDYGSKDCYYLATFSGGEKND"
     gene            7962..8126
                     /locus_tag="ACL73L_000919"
     CDS             7962..8126
                     /locus_tag="ACL73L_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771918.1"
                     /translation="MVGVTYQEIHLFVEFLKEQYGQGRPDYIEALNDLDGLVEVSYRE
                     AIERFLEDEL"
     gene            8116..8325
                     /locus_tag="ACL73L_000920"
     CDS             8116..8325
                     /locus_tag="ACL73L_000920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771919.1"
                     /translation="MNYDKRTVIDGLKRTIEQNEEKIIEYSKPCDARKRRIRALERDL
                     LKKKNKELRRKAEELEDDGRVKEKS"
     gene            8297..8614
                     /locus_tag="ACL73L_000921"
     CDS             8297..8614
                     /locus_tag="ACL73L_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771920.1"
                     /translation="MMEELKKKVNEVYNWTVEDGKPQPPKQDLPQAVKDRADYFWEMA
                     EDGMTFMGAMECIFADEKPTDYDLGATKGWLPKSKEFDDWVGYSPGMSQVVIAVYLIY
                     GGN"
     gene            8616..9119
                     /locus_tag="ACL73L_000922"
     CDS             8616..9119
                     /locus_tag="ACL73L_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HGS0771921.1"
                     /translation="MNKQELIEKYKKLEGVWNAEGAELARQIFLQDLEQLDKPKPVKV
                     PQCVAEYIEFKKKNNFHVYGAMRVIEDHYDKKVPEWFYENNIEKFCLAWLDGYEVEKE
                     KRYFVKIKGNIKENMLVYGELLKRYFFTKSFSLDDVIYSHTRKELEDANFGWVFDCEG
                     FEIEEVE"
     gene            9119..9406
                     /locus_tag="ACL73L_000923"
     CDS             9119..9406
                     /locus_tag="ACL73L_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000194861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771922.1"
                     /translation="MTQTLEEGMKNQSKCIKVPREIRPFDIGYRIVNKYGQALALRNG
                     ASIFDLPFLAEKAIEKEFGKNDPDFDIGKHSVEEVAIVNLSKFHSYFEEVE"
     gene            9445..9567
                     /locus_tag="ACL73L_000924"
     CDS             9445..9567
                     /locus_tag="ACL73L_000924"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771923.1"
                     /translation="MAIGEIIVGSDGKTTTAILEIPDQSFYLEIELKLKEEVIN"
     gene            9564..9824
                     /locus_tag="ACL73L_000925"
     CDS             9564..9824
                     /locus_tag="ACL73L_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1372 family protein"
                     /protein_id="HGS0771924.1"
                     /translation="MKRFIAIWILLSAGLNIWQSIYIKKLEEKRPIVVYKADNAGAEI
                     FGKVVEKGRHGKLYTLTIRDYGVFVVTKDVYEKVKVGDEVML"
     gene            9821..10903
                     /locus_tag="ACL73L_000926"
     CDS             9821..10903
                     /locus_tag="ACL73L_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HGS0771925.1"
                     /translation="MKFLDLFAGIGGFRLGMESAGHKCIGFCEIDKFARESYKAIHDT
                     KGEIELHDITAVSDESIRGIGSVDIICGGFPCQAFSIAGNRRGFEDTRGTLFFEIARF
                     ASILRPKYLFLENVKGLLNHENGVTFETIISTLDELGYNVEWQVLNSKNFGVPQNRER
                     VFIIGHLRRERTRRFFPLSGKNQSTSNQSVMKIGNINPSGNGMNGEVYQADGLAPTLT
                     TNKGEGQKIAIKSNTIKQFGVLQPNFYQCGVVYETDGIAPTIRAYQGGGLEPKIIQRG
                     HGYNQGGEHDIAPTLTSNSYHENNHLSDGFRIRKLTPRECWRLQGFPDWAFDKAQEVN
                     SNSQLYKQAGNSVTVNVIAAIAKELS"
     gene            10900..11283
                     /locus_tag="ACL73L_000927"
     CDS             10900..11283
                     /locus_tag="ACL73L_000927"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771926.1"
                     /translation="MNTIEKVKQWFIDRDLENGRVVGLMVLSSKRRIYKMKRLGIIIG
                     AVFVIVVSPFVVQYGWNEIITTIVPVGKITVWQALGMDALLSFIWPVLSSKKESEEDY
                     SYAVKSSISKIITCAFLIWLASLFI"
     gene            11295..11696
                     /locus_tag="ACL73L_000928"
     CDS             11295..11696
                     /locus_tag="ACL73L_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HGS0771927.1"
                     /translation="MIPKFRGLSIDEKSKGEWQYGHLIEDRGRAFIINEVVEANEQYI
                     TIGSWCPVNPATLGQSTGLRDKNGKEVFIGDIVKCTRGCLHEVYLEKEYGGTFIGGMP
                     SIYLKGLLSGYAWTEDEEIIGNVYENLVYIF"
     gene            11714..12115
                     /locus_tag="ACL73L_000929"
     CDS             11714..12115
                     /locus_tag="ACL73L_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGS0771928.1"
                     /translation="MKKLSDEDLKTLDRELFKFQNIQRTIDLRRLELETRNPDAQSSP
                     SVGISKPTETIAVRIADDPTLKFLEGFKAIINKLLINLVDEDKEIFNLRWRYPQLRWE
                     EIAEQKFMSKATIYRRRRIILEQYAILKGEL"
     gene            12306..12848
                     /locus_tag="ACL73L_000930"
     CDS             12306..12848
                     /locus_tag="ACL73L_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000397549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HGS0771929.1"
                     /translation="MEFVSPIKDNDDIQAMKDYLREWNEMYYMLFITGLNTGLRVGDI
                     LTLKVKDVQGWHIKLRERKTGKQITRRMTKELKKEMRRYVEGKPFHHFLFKSRQGQNK
                     AITRERAYQIIHEAAEELGIDNVGTHTMRKTFGYKYYNKTKDVGTLQKMFNHSSPAIT
                     LRYIGIEQAELDDALRNFVI"
     gene            13225..13569
                     /locus_tag="ACL73L_000931"
     CDS             13225..13569
                     /locus_tag="ACL73L_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HGS0771930.1"
                     /translation="MYNKPIRPSLKSKKWEKFRDRIMRKHDYLCQESLRYGISVQAEM
                     VHHIFPVSEYPELEFVEWNCLPLTNKKHNTFHDRVNDRVINQGLYWQKKRKKEFLIPP
                     LFEKSFWPVGYR"
     gene            13649..14041
                     /locus_tag="ACL73L_000932"
     CDS             13649..14041
                     /locus_tag="ACL73L_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HGS0771931.1"
                     /translation="MAKPITAKSIKSKVVKQMKDLGTYRKEFEMIIDIFAGMLYQYQK
                     LAQDYADMGYPVTDTYVNKAGAENERKVPILTAMEILRKDILSYSNQLMMNPKSLGEV
                     VEQEGESVLTEVLKFKNEIKKKRVTGNG"
     gene            14034..15764
                     /locus_tag="ACL73L_000933"
     CDS             14034..15764
                     /locus_tag="ACL73L_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HGS0771932.1"
                     /translation="MGNLDKAKKYAQHVLTHREEHCEENILAAERFFRDLENPTFEMD
                     EDMVDFVIHFIENVIVHQQGDDMFAVSIRNKPLLLQPWQHFVVVNLFGFYYKGTNERR
                     FKEALIMLARKNGKTSFTAAIALAYQILDTDSGSKCYIVANSVKQAMEAFGFLRFNVE
                     RWNDKNIRIKDNNQEHSITANFGDEGSFFIQALANDESRLDSLNGNVIILDEAHTMRN
                     SKKHGLMKKTMSAYRNSMLFVISTAGDIPTGFLANRLKYCQKVLKQLVTDDSFFIFIC
                     KANQSADGDVVNYLDENILKMANPSWGVTVSFKALKEEAEQAMNDPQTRNEFFNKTLN
                     IFTNSMNAYFNPDEFIASDSCYDWSLEELARLPIRWYGGADLSRLHDLTAAALYGVYH
                     DGEKDVDICITHAFFPRINAQKKANDDGIPLFGWQSDGWLTMSNTPTVLYDDIVKWFI
                     KMREKGFKIAAVGMDRKFGREFLTKMKQARFKMIDQPQLFYLKSEGFRRIEFKVKNKE
                     FYYLHSDAYEYCVSNVRAIEKVDDAVQYEKLDGDGGTARIDLFDASVFACIQALANLG
                     KGGDVMRFFD"
     gene            15772..15990
                     /locus_tag="ACL73L_000934"
     CDS             15772..15990
                     /locus_tag="ACL73L_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771933.1"
                     /translation="MNEIVLSEHEINLLINKGRVKVILNGEVVTIRQRHMKNLMAETV
                     KWEKQVIDVSQNIVRNKHFDSLFQNTFR"
     gene            16008..17210
                     /locus_tag="ACL73L_000935"
     CDS             16008..17210
                     /locus_tag="ACL73L_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGS0771934.1"
                     /translation="MGIFEKFWKRNKPSKSINMLSHSDLGLSNLMDSYVPLARNPDVV
                     TAVNKIADLVSNMTIHLMENTDKGDIRIRDGLARKIDINPCKHMTRKSWIFKIVRDLL
                     LYGDGNSVLHVEYEPVTDYISNLRPFPMREVSFQTDKDSYVISFRGEEYSPDEVVHFV
                     INPDPDIPYIGTGFRVTLTDVVQSLNMATKTKKSFMNGKNIPSLIVKVDSSSAELDSE
                     QGRERIAEKYLSTSRVGAPWIVPEALLDIQQVKPLSLTDIALNESVELDKRTVAGLLG
                     VPAFILGVGEFNKTEYNNFVNTTVMSIATTITQTLTRDLLLSSNRYFKLNPRSLFSYN
                     ITELSAVAQQMANSAAMRRNEWRDWLGMAPDPEMEELIVLENFLPQEKLGDQNKLKGG
                     EEENAKAK"
     gene            17194..17769
                     /locus_tag="ACL73L_000936"
     CDS             17194..17769
                     /locus_tag="ACL73L_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HGS0771935.1"
                     /translation="MQKRNSYRATQFQTREEESGDLVLSGYFIKFDEETELWRGYHEV
                     IKRAGVEKAVTDADIRALFNHDDSLVLGRTGNGTLTLGVDDVGLFGDIIINKDDPQAV
                     GAYARVKRGDVIGCSFGFIPVKIETEEREDGSYLDTVLELEIFEVSPCTFPAYPQTEI
                     AARQKDFESQKRANREALDKRKKEIKEKFKL"
     gene            17766..18932
                     /locus_tag="ACL73L_000937"
     CDS             17766..18932
                     /locus_tag="ACL73L_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HGS0771936.1"
                     /translation="MNKALIFGARMRAKATKVVELEETIEELNKRSVVELEKLDRAKN
                     DEEVLAVEKTVDGLQREIEEKEAEKVQLENEIDELDKQIKEQNRKAPTPGKMEERGGK
                     TLGQREAFNHYLRTKEARADGFKSAEGEAIIPVELMTPKEAKQDKTDLTSLVNIVNVK
                     NASGKWSVVKLTDQEMNTVEELEENPELAKPTFTKVNYEIQTRRGHLPVSQEFIDDAD
                     YDVMGLVAKQTKNQERITKNKEIAKVLKTATSKSAAGLDGLKDILNVELKTYYNATIV
                     CTQSMFAALDKIKDKDGRYMLQTDITSPTGYKFAGRVIVVYPDDIIGESKGDLKAFIG
                     DVGEFATLFDRAQTTVKWQDDKIYGQYLATANRFDVVKVDGDAGFYVTYTDAVL"
     gene            18944..19213
                     /locus_tag="ACL73L_000938"
     CDS             18944..19213
                     /locus_tag="ACL73L_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771937.1"
                     /translation="MVYKVIRPFKDLRDPKKHCYKVGDNYPRVKHKLDKEFAETLLNG
                     ANSAGSIFLMNVEDKDMSKDDTLTTEVEKEETKVVDGKPEEGDEE"
     gene            19216..19497
                     /locus_tag="ACL73L_000939"
     CDS             19216..19497
                     /locus_tag="ACL73L_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000371962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771938.1"
                     /translation="MDNVQLLELLKLKLGIATKLRDKPLEKIIEAVKTELEDNLGVLL
                     DLDSSEDQMFVVDFAAFRYEGGVDMPRHLQWRLHNLQIASKKEVKNVES"
     gene            19484..19783
                     /locus_tag="ACL73L_000940"
     CDS             19484..19783
                     /locus_tag="ACL73L_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HGS0771939.1"
                     /translation="MWNHEITLISKEVTGKDKLLQPISEDVEVTLLCRKKRVTRSEFY
                     QANQVGLKPSLVVEIRNFEYENQELAKFEGKQYHILKTYPIDSEILELTLSEMLK"
     gene            19780..20139
                     /locus_tag="ACL73L_000941"
     CDS             19780..20139
                     /locus_tag="ACL73L_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HGS0771940.1"
                     /translation="MSLTSDLANEIAKAMAEYSAEVEDKIDLIAEDVVNEAVTELKAT
                     SPKRHGKYARNWRFKKNAKGSYVIYNAAPTYRLTHLLENGHVLRNGGRSRAFPHIKPV
                     EEKVKENFEKRIKEIGK"
     gene            20136..20459
                     /locus_tag="ACL73L_000942"
     CDS             20136..20459
                     /locus_tag="ACL73L_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771941.1"
                     /translation="MKLSDFAAILEQANLPVTYRAFKTGNAPDLPYLVYYESIPAINA
                     ADNTVNHQIKSVTVELAFESKDEDLEERLEELWTTHELFFDVQEETFIEIERLYVKSY
                     TVYLY"
     gene            20471..21049
                     /locus_tag="ACL73L_000943"
     CDS             20471..21049
                     /locus_tag="ACL73L_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HGS0771942.1"
                     /translation="MNQENKVTFGLKNVHVAPIKSIGADGVIAYDEIFRFPGAMELTL
                     DPRGESIPIKADDIDYHFMNSNEGYEGKFKISHIIEMFATKILGEIKDAQTGVLTEKA
                     DAEFTSFALMFEFSGDKNKTRHVLYYCSASRPGNGSKTKNGTNVNERELGFKASPRPL
                     DSVVKRSITSADNKEIYDNWFKKVYEPTAVAA"
     gene            21061..21480
                     /locus_tag="ACL73L_000944"
     CDS             21061..21480
                     /locus_tag="ACL73L_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771943.1"
                     /translation="MRKIVWVGVQEYELGTNGYTPIAYKQQFGKDYFQDLFSMLKNQS
                     FMNELNKLETDKELTATDIDISMLSDFDMTFFNRLFWTFAKSANPHIKPYEQFFMEME
                     VFPIQEVGPVLMEMLNASMTTKKHQMNQNQLAKKSSQ"
     gene            21757..24507
                     /locus_tag="ACL73L_000945"
     CDS             21757..24507
                     /locus_tag="ACL73L_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGS0771944.1"
                     /translation="MAGNIKGIKIEIDGDTQPLQKALKNVNKAATDASQELRQIDKAL
                     KFDTGNVTLLTQKQEVLQKQVSTTKEKLETLRQAQSQVEQQFKNGDIGADQYRAFQRE
                     VEVTQNVLKGYEGKLANVNQALNENGSATQNNKNQLKELQNEQSQLASEMVKVTSSFK
                     LQESALGSNASEAERNALAQKKIGVQSEIVSKQISNLEQQLEITKKEFGENSTQANRM
                     EAELNQAKTAFNHLNDEMKGTKSAADSTQESLSEISRNLRAELLQQFSEKLSAISEKL
                     VEVGKEALEAAAQMQASNAQFTTVFGDMETQAREALNAIGQEMDIVPERLQGSFTQMA
                     SFAKTSGLDTAEALDLTSRATRAAADGAAFYDKSIESVTESLQSFLKGNFANDAALGI
                     SATETTRNAAANELYGKSFKDLSEAQKQLTLLQMVEDGNKLSGALGQAARESDGLENV
                     MGNLKQAGTNALSAIGQPLLEMMIPVFQTLATIVKGVAELFNSLPDPVKDFIVILGVA
                     LTIVGALAPIFLTLQAVFMSSFGAMIAAALPIIGIISGVVVAIAAIVAIVKYLWETNE
                     GFRDAVTTVWNAILEVINAVVSEISNFVMSIFGTVVAWWTENQELIRASAETVWNAIY
                     TVISTILEILGPLIQTGWDNIQLVITTAWEIIKTVVETAINVVLGIIQAVMQIINGDW
                     SGAWETIKGVFSTVWQAIQSIVQTIFSAIQSYISNVLNGISGTVSNIWNGIKDTVSNV
                     LNGISGTVSSVWEGIKSTISGAINGARDAVSSAIEAIKGLFNFSISWPHIPLPHFYVS
                     GSANPLDWLSQGVPSIGIEWYAKGGIMTKPTIFGMNGNNIMVGGEAGNEAVLPLNDKT
                     LGAIGRGIAQTMGGTSPTINITITGNTVREEADISRIADEVAQRIADELQRKTQLRGG
                     FT"
     gene            24504..25226
                     /locus_tag="ACL73L_000946"
     CDS             24504..25226
                     /locus_tag="ACL73L_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGS0771945.1"
                     /translation="MIKHNELVIDGVRTSSFPFKVIVHDSPSIALGESKTALLEHGGI
                     SGAIVQTNKHRELVKKTYTIYLVKPTEEQMNQFMSLFIREKFWLESERVKTTRLWCYK
                     VNVSDLEEVQPGLYMTKATFTCHPTKYFKGSDTQRLTRSGTLTVQGSALAFPKITIVG
                     QSAVETSFTIAGQVIRLEKLSESLVMVNNPDNPSFKTTTGKPVKWSGDFITVDPAKVK
                     NIGVVLGPGIQSIEIETVWGWA"
     gene            25227..>26734
                     /locus_tag="ACL73L_000947"
     CDS             25227..>26734
                     /locus_tag="ACL73L_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000966215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HGS0771946.1"
                     /translation="MLYLLNKDVRTVRWNGEPLHEATSAIVKEIMNGDFTLTVKYPIS
                     DSGIYQLIQEDMLIKAPTPVLGAQLFRIKKPVEYNDHLEITAYHISDDVMQRSITPVS
                     VTSQSCGMALSRMVQNTKTALGDFSFNSDIQDRRTFNTTETETLYSILLDGKHSIVGT
                     WEGELVRDNFAITVKKSRGENRGVVITTHKNLKNYQRTKNSQNVVTRIHAKSTFKPEG
                     AEKETTIRVTVDSPLINSYPYINEKEYENNNAKTVEELQKWAQSKFSNEGIDKVSDAI
                     KIEAYELDGQVVHMGDTVNLKSWKHNVDAFKKAIAYEFDALKEEYISLTFDDKAGIGG
                     SRASGGLSSAADAILGVTESAQEIALEKALQNADLDFDHKAGLLRQEISDDIELAKAK
                     AEEVKRELSDTINQRFNSFDNGPLKETKRKAEEALRQAGASSSLAQEAKRIGLDSVAR
                     LEAFKSQTTSAQTALSGDLDALKRTIVNDIRPKQAQVEAEIAKQVEALVQTK"
BASE COUNT         8673 a         4477 c         6105 g         7479 t
ORIGIN      
        1 ataatttttc gatatttttc ggtatttttc ggtttttggt cggggaattg gcggggactt
       61 tttttagcga atatgactaa gaaataggtc tgtggtcgct tcagcaagtt cgtcctctac
      121 ttgattgtaa cgatccgtca tataaacctt tgtatgcccc agcgcctggc ttaattgttc
      181 aagcggaacc cctgcaataa tgctttgagt cgtgaagaag tggcgcatca tgtgaggtgt
      241 tacatgcaat tctgttgctt cattcactag attgaagttt ctatttagct gatttggatt
      301 gatgagacca cctttctcgt tgatagttat ataatccttg tgctgttcct tgataattcc
      361 taactttcgc ttaatcttag aagcttcagc tatcagataa tagatcaggt ctgttccgat
      421 atcatcaagg cagacatatc gctctgaatc cttcgtttta agccctcctt tcccttttaa
      481 ggtctggttg cttcgactgt ctctaagatg cagtatagcc cgtccgctgt cgttctgagt
      541 gatgtccatt ggacgcaatc caaagacttc tcctcttctc aatccaaaaa ttgtcagata
      601 ggtcagagcg tagaattgtt ttggcataat ctcttctgcc tttgctatcc aagtcttgaa
      661 ctctttgaga gtcactttct tgtttgcagc agggatatca ctctggccga tgaaaacacc
      721 tttcaagcga tttgagagca gatttccatt tttgacggca tcattcagca atgccatgaa
      781 gctggaattg agggtttgaa cagtgtatct ggtatggttc tgcaactttt cagcgataaa
      841 gagttcatac tcatttctat ccaaattttt aagctgaata gaaccaaatt tggttttgat
      901 atgattctta tagaggttat cattgaggta gtaggaagtg tcattccagc gccctgttga
      961 caatctcttt tcagaataaa tatcccaata ttgatcaagt gtcagattcg tattgatacc
     1021 taattcttgt tcttggattt gttgctcaag ctctaccaag gctgcacgag cttgagggag
     1081 agttgtgaaa ccacttttac ttttttctct ttttttacct cggaagaaaa aagaacgtct
     1141 gacatagtaa cgcttgcctt tagcagtctc atagtaatag atatttgggt attttgtttt
     1201 attatatttc attgtattct ccttgtttat cggcttctgg acaaggtcta aacattgaga
     1261 atattgacat cacccctttc atggtgtaaa atagggtata gaaaagaggc ctttttaatg
     1321 gctgattttt tataagggtg ggcttcacaa tcaaactttg gcgagggaga ttgtggagct
     1381 tttttgtttt taattcaaca aaaaacggta actaaattta tagttaccgt ttctgcgtgg
     1441 cagcttgtgc caaccaggtt atttgcacta ggatttcccc taggttagta actatatatt
     1501 atcaaatata tttcattttg tcaaaaatgg aaagttgagt cttcacactc agaatttaga
     1561 gatagagagt gtggggattt ttttatttct tgactttatc ttttaaagcc ttctctatgg
     1621 ctttctttaa ttccaaaata gatgcttttt catctttggt aaaggttact gtgttttcat
     1681 cttttacggc atcaaacaca ccaccttttg tatcagaaga accagggtaa acaagttgaa
     1741 gatagccaac agttgagcca ggttctttca attggtatgc tgtaatctca gagaggagaa
     1801 ttgacttttc gccatcaaga ccatgaagaa gaacattaga tacatttgac tttcttgcaa
     1861 tcctgataaa ataatcatcg attcttacaa cagtttttga tttcttaaac tcaaaaactc
     1921 gctcattcgg ttcctctgtg aagagttcaa cctctaaact ttcatcttgc tttttaccaa
     1981 acaatgccat aagtagttcc tttctttttc tgcttcagca gtttataaac atatttaacc
     2041 aattaaagtc tgatattcct cttttaccat gacttcattt gtcacggttt taagattgta
     2101 gtaggacatg aatttgaggt aatcaaactc tttggggtcg tctaagcttt ctagtgcgtc
     2161 ttttacgaga tgatggatca tattcctatc agcttcgttt tcacagcgta agcgagcgtt
     2221 ctggtattct gatcgtgtgt gatccttgtg tcctagttca tgaagtagga ccttaactct
     2281 ctcttttttg ctgagtttat tagacaagaa agctgtattg gtttcttttt cgtaaaatcc
     2341 aagttcatca ggtattagct cgccgtcaaa atcgacaatg cgaacttgaa aatgacttat
     2401 aatttctttt tcggtcacta agcagtacct ctaatcacca gcttctttga gataaccttc
     2461 aatgatagac tggatgattt tcttcttttc atctgttaat tctcggccac caaacatcat
     2521 gacattagat gccatttctt caacattgag aactttgccc tgccatatgt attcttttgt
     2581 atcatcggca agattaggat tttcagtacg gcctagtaag tagtctgtac taacaccaaa
     2641 ataatctgct atttcttgta atctatcaga ttttgggtta ccttttttca gactataaag
     2701 ataatttgtg ctgtatccta atttttcttc taaaatgttt aaagaaattt tctgtttatc
     2761 agccaattct ttgattctat cgaatgctaa gaacattgtt ttatcaacct ttctaagcac
     2821 tacgaaaaaa atattttaaa attaattata aaaactgttg acaaattcta aaactagttt
     2881 taaaatagta ttcgtaagct aaagagttag cgaactagac aactaaaaaa taaagcctta
     2941 caaaactgat tggcgtccgt tttctatagg taaaacttac tttagtaggt cttttctcta
     3001 tgtctttatt ctaaaactag ttttagaatt tgtcaagtag ttcgctaact ttttagataa
     3061 tttttttaaa aggaggtcag ggatgaacga agaagacctg aaagaattat tggaactctt
     3121 agcgacagat tatgggcgag ggtatctaga tggagtagtt gggggacttt caatgctttt
     3181 gaaaatttca aaagaagcag agaagaatag aaaggaagaa tatgagtaaa gcaaaattat
     3241 tagtcacata aagtaggtaa acgaggtagg aaatgataga aacactggaa ataaaaataa
     3301 acaaaaagac atacttcata tctgcaaggg cagatgttcc agtcaggtat aaagttatat
     3361 ttggcagaaa tatcttcgat gacttttcga aaataaaaaa caataaagat gacactctat
     3421 cactttgtct attattttgt atgacagaca aatcgtcaga agaattctgt aaatttttta
     3481 ttgaaaataa ttcatatata acaaataaac atcactatga ggcaataaga ttgtttgaaa
     3541 aacattttat ctctaagcgc gtaacatatt cattagagga tatagttcaa atcctaacaa
     3601 tatatctaga aaatactaaa acacagattg gaggaaaaaa tgagtaaaga actaaaaata
     3661 attaaggcta aaattaaaac tcgtttgatt gagttggata tgactcaagc cgaattggca
     3721 aaacaagtat ctgtagcatc atcagttatt tcagagctac tgaaatatgg aaaaggaagt
     3781 gattatgtga aagaaaaagt cgtagatatt ttgggtattg aaaacccttg gagaaatcac
     3841 tgagaggtcc gtacatgcaa gcgaaaataa tactgaattg gcagaagaaa aatcaccaac
     3901 ttagtcagat gatgatcgat agtcttgagg gactagatgt ttgggaaact attttaacac
     3961 taggaaaagt aagaagagga acattatgaa cgaaattttt aattttcaag gacaggaagt
     4021 ccgtactttg acaattgatg acaagccttg gtttgtcggt aaggatgttg cggatatctt
     4081 aggatatgcg aattcaagaa aagcaatttt tgaccatgta gatgatgaag ataagacaga
     4141 tggggtaacg attcgtgaca ccatgggtag aaatcaaaat cctatcatca ttaatgaatc
     4201 tggtctctac tccctcatct tatccagcaa gttgcctcaa gcaaaagagt tcaagcgttg
     4261 ggtgacatca gaggtcttgc cagctattag aaaacaaggc ggatttatcc gagaggactt
     4321 ggatgaagat gctttcatcg ctctgtttac tggacagaag aagttgcgtg agcaacaagc
     4381 gactatgctg gaagatattg actacctcaa gagtgagcaa ccgattcacc caagctatgc
     4441 taaatcgctc ctgaagaagc gtaaggctcg ggtcgtggct tgcttaggtg gcattgatag
     4501 tccggcttat gcggataaga ctttcgctca gtcagtcttt agacaagctg agattgactt
     4561 taaagaccac ttcaacatta atcgctatga cttgctaccg aaaaaattcg cagatgcagc
     4621 cttggcctat tggatgactt gggagccaag caccaatacc aagatgaaaa tcatggaatt
     4681 gaactcattt gacgaagggt aggaggggaa gaagatggac aatgttctac tttcactgtc
     4741 tgaatggatt aaatccatta tcaaggacac aatcacaagg ctagtcgaaa tagaaaaaga
     4801 tagtgaccac tatccagagc tgatggatgt gaacactacc tgtgattttc taggaattaa
     4861 gtatgccaca ttttcagata attatcgtta cttaaaggga tttccaaagg aattacctgg
     4921 taagaaatgg tcaaaaagag ccatcaaaga atggctctct aatcaaatat aataacttta
     4981 ctaaaaggct tctggacaag gtcttagcaa aattatttga ctatattata gcacaaaaag
     5041 aggataagga gataaaaatg tttgaaccac cgattttaga ccagctgatg ggggttggag
     5101 ccttactgct tggatttgca ggggcttgcc gtcatatcaa attgcaggaa caacgcaagg
     5161 aagaagaaag acgagaagag caagaatttg cgtctatgat tattcgaggc tataaccatg
     5221 cttacgaacg tggtagagag gctgaacgtc aagaaatccg caagaatatt cgtcgtccgt
     5281 tcaagggctt tacctacgac aatgaaccgc ctataggctt gcgtcctgag ccgttagctt
     5341 tgccagaacc taaacagtct gcaatcagat ttttgtaatg aggaggtcag gaaatggaag
     5401 aattgattga atggctggat aacctgatta tgattgttaa agaactggaa ggaagggaat
     5461 caacttcaag acattttatt acgatatggg aaaacgatta taaaaatcta ttactagtca
     5521 aagaatacct aaccgactat gaaaaactag caaaggacta tcgttatgtg acccttaaaa
     5581 ataagctgct aaagattgaa aaaatggagc tggaaggcag gtacatctat gaagatatgc
     5641 ggatgaagta tcgcgctaac cgtaggaagt ggggtgctag gtatgtctga aattaagtgg
     5701 ataaaaatca caaccgatat ttttgacgat gaaaagattt gcctgattga tgccttgcct
     5761 gatcctgatg ccatcttagt gatatggttc aagattttga cacttgctgg aaaacataac
     5821 agtaatggtt tgttgatgat gactgataag gttcactata cagatgaaat gttagctact
     5881 atttttcgta gaccattgaa tacagtaaga atggctattg gagtttttga acagtttggg
     5941 atgattgaga ttatcgatgg tattattagc ttgccaaatt gggaaaaaca tcaaaacgtt
     6001 gacggaatgg agaaaatcaa ggaacagaca cgtaaccgtg tagccaaata ccgtaaaaaa
     6061 cagaaaaatc ttgctcttgg taacgttaca ggtaacgtta cagtaacgga cggtaacgca
     6121 ctagaagaag ataaagataa gaataagaat agattagata aagataagaa taagaaaaga
     6181 ataactacta ctagtagtgg tagtgaagaa aatatcttag aactttttca atctgagttt
     6241 cgtagactct tatctggatt tgaaattgaa gaaatcaacc atctactaaa tgagaataat
     6301 gtggatttgg tgaaagaagc attgaagact gctattaact caggaaagcc gaacatcaaa
     6361 tatattggtg ggattttaag aaattggcag atgaacaatg ttaccactgt tgaacaggtt
     6421 cgtcaatcgg aaaagaagaa caaggataag aaagaagaac aggaggccaa ggacgaatgg
     6481 gggtactaga actaattgaa caattcgaga ttgactatta tccgttaagc tacgagaaga
     6541 aaactctttt agcagaccaa ccaattcatc aagtggttgc ctgcttgtct gaaatggcta
     6601 gctggcatga atgcggaggt cggctagtat ggtagacaat gtgtttgagg aaatcgcctt
     6661 atcttatcgt aggaatacag aacaacaaga agagttctgc gaaaagcata acatcccttt
     6721 gataaagata ttgaggaccg agagtgttgt atgtcgcatg tgtgaatctg agcggattca
     6781 tgaggaaaat caagcaagag tgaatgaatt ggccgacgct gagaatgagc gagagaggaa
     6841 atactatctt gagaagtttt ctctttatga tgaggttttg aaaaatgcga ctttggacaa
     6901 ttttgaaaca ccaactgaaa aagaagcaga aaagctagtt tttgcaaaga ggatttgtcg
     6961 tgagtggtct gagggtgcta ggaacaacat cgtgttacaa ggagaagctg gaacaggtaa
     7021 gagccatttg gcctttgcga tggttaaggc tctatctgag tacacgaaag agattgctat
     7081 cttcatcaac gtgacggact tgttgatgaa gattaaagct gattttagtc aggaggagtt
     7141 tctggtcaat aaaattgcca gtgctaagtt cttggttttg gatgatttgg gaatggaaaa
     7201 ggatagcgaa tggtcgttta ctattctcta caatatcctg aataagcgtt caaatacaat
     7261 cattaccacc aatttgactt ctgctgatat tcagaaaaga tatggcagac cctttatgtc
     7321 cagactgatg aagggtgtgg ataaagacca tttaatggtt ttcaacgact tgacaaacaa
     7381 gcggaagcaa tatttttaga atggaggtgg ctgatgttta ttttaagaca tgggacaaga
     7441 gaggataagc cgtttctgag gtccgtagtt attggtgtga ctggcttgga catttcatgt
     7501 tcagaggaga agaaagccat gcggtttgtt tctcgtgggg cagccgtaca ggttggtaag
     7561 gctttgaggg gttcctttgg gaatttttat cccgttgagg tggagtgatg ttagagcttt
     7621 acttcgtcta caacgggcac tgcaagtttt accttggaac gtttgacaat gtcgatgatc
     7681 tcattgaaca gatggaagat catcagtggg ttttctcggc tatcactcat ccaagatttc
     7741 agaagcacat tggtcagcgg acgacacggt ttgactacgg ttcgaaggat tgttactatt
     7801 tagcgacttt ttcaggagga gaaaaaaatg attgaactta ttaaagaatt tgtaatggct
     7861 attctgtggt tatttctcgg ctatttagtc ggggaacgtg cagcaagaaa ggaaaagaaa
     7921 gatgatcaat aacgttacat ttttagtggg aggtaagaaa tatggttgga gtaacctatc
     7981 aggaaattca tctctttgtt gaatttttga aagagcagta tggacaaggg cgtccagact
     8041 atattgaagc cctgaacgac ttagacggtc tggtggaagt ctcctacaga gaagctattg
     8101 aaagattttt agaagatgaa ttatgataaa cgaacagtca ttgatggact gaaacgcaca
     8161 atcgagcaaa acgaagagaa gataatcgag tattcgaagc cgtgtgatgc acgcaagaga
     8221 cggattagag cgctggagcg cgatttgttg aagaaaaaga ataaagaatt aagacggaaa
     8281 gcggaggagt tggaagatga tggaagagtt aaagaaaaaa gttaatgaag tatacaactg
     8341 gacggtagaa gacgggaagc cgcaacctcc caagcaagat ttaccacaag cggtgaaaga
     8401 ccgggcggac tatttttggg aaatggcaga agatggtatg acgtttatgg gagcgatgga
     8461 atgcatcttc gctgatgaaa agcctacaga ctatgatttg ggagctacta agggttggtt
     8521 gccaaaatct aaggagtttg atgattgggt tggctattcg ccaggaatgt ctcaggtagt
     8581 tattgcagtt tatttgattt atggaggaaa ctaagatgaa taagcaggaa ttgattgaga
     8641 aatataaaaa acttgagggt gtatggaatg ctgaaggagc agaactagct cgtcaaattt
     8701 ttctgcaaga cttggaacaa ctagataaac caaaaccagt caaagttccg cagtgtgtgg
     8761 cggaatatat agaatttaaa aagaaaaaca attttcatgt ttacggtgca atgagagtaa
     8821 ttgaagatca ttatgataag aaagttcctg agtggtttta cgaaaataac atcgaaaaat
     8881 tctgtcttgc ttggcttgac ggctacgagg ttgaaaaaga gaagcggtat tttgttaaga
     8941 ttaaagggaa tattaaagaa aatatgttgg tttatggaga acttttgaaa aggtatttct
     9001 ttacaaaaag ctttagttta gacgatgtta tatattccca cacccgtaaa gaactagaag
     9061 acgcaaactt cggctgggtg tttgattgtg aagggtttga gattgaggag gtggagtgat
     9121 gacacaaaca cttgaagaag gaatgaagaa tcaaagtaaa tgcataaaag tcccaaggga
     9181 aatcagaccg tttgatatag ggtatcgaat agtgaacaaa tacggtcaag cgctcgcttt
     9241 aagaaatggg gcaagtatat tcgatttgcc ttttctggct gaaaaagcta tagaaaaaga
     9301 atttgggaag aatgatccag actttgacat cggaaagcat tctgttgaag aggtcgctat
     9361 tgtcaattta agtaaatttc atagttattt tgaggaggtg gagtgatgag ccttacgcta
     9421 aatagcacaa ttggagactt agttttggca atcggagaaa ttatcgttgg ttctgatggt
     9481 aaaaccacta cagcgatact ggagatacct gatcaaagct tttacttaga gattgagctt
     9541 aaattgaagg aggaggtcat aaattgaaac gattcatagc tatctggatt ctgctatctg
     9601 ctggattgaa tatctggcag agtatctaca ttaaaaagtt agaagaaaag cgcccgattg
     9661 tcgtctataa agctgataac gcaggcgctg agatattcgg taaagtcgtc gagaaaggac
     9721 ggcatggcaa gctatatacg cttaccattc gtgactacgg ggtgttcgtg gttacgaagg
     9781 acgtgtatga gaaagtgaaa gttggggatg aggtgatgtt gtgaaattct tggatttatt
     9841 cgcaggaatt ggcggttttc gtttaggaat ggagtctgcc ggccataaat gtattggctt
     9901 ttgtgaaatt gacaagtttg ctagagaaag ctataaagcg atacacgata cgaaaggaga
     9961 aatagaatta catgacatca cagcagtatc agatgaatct attcgaggaa tcggaagtgt
    10021 ggacattatc tgtggaggat ttccgtgcca agctttctca attgcaggaa acagacgagg
    10081 ttttgaagat acacgaggaa ctttgttctt tgaaattgct aggttcgcat ctattctcag
    10141 acctaaatat ctattccttg agaatgttaa aggattgctc aatcacgaaa atggagttac
    10201 attcgagacc attatctcaa ccttggatga actggggtac aacgtggaat ggcaagtgct
    10261 taacagcaag aatttcggag tcccccaaaa tcgggaacga gtgttcatta tcggacatct
    10321 tagaagagaa cgtaccagaa gattttttcc tctcagcgga aaaaatcagt caactagtaa
    10381 ccaatcagtc atgaaaattg ggaatataaa cccatctggc aacggaatga atggggaagt
    10441 ctatcaagct gatggtctag ctcccacgct aacaacaaac aagggagagg ggcaaaagat
    10501 agctataaaa agcaatacta taaaacaatt tggggtattg caacccaatt tttatcaatg
    10561 tggggtggtt tacgaaacag acggcatcgc accaactatc agagcctatc aaggtggagg
    10621 acttgagcct aaaattattc aacgtgggca tggttataat caaggtggag aacatgacat
    10681 cgctcctact ttaactagca atagctatca cgaaaacaat catttatcag atggctttcg
    10741 aatcagaaag ctaacacctc gtgagtgctg gagattgcaa ggattcccag attgggcttt
    10801 tgacaaagcg caagaggtca actctaacag tcaattatat aaacaagcag gaaatagcgt
    10861 gacagttaat gtcatagcag caatagcaaa ggagttatca tgaacacaat agagaaagtc
    10921 aaacaatggt ttattgaccg tgatttagaa aacggtaggg tcgttggatt gatggtgctt
    10981 tcgtcaaaga ggaggattta taaaatgaaa agactaggaa tcattattgg ggcggtattt
    11041 gtaatcgttg tatcgccatt tgtagttcag tatggatgga atgagattat cacaacaatt
    11101 gttccagttg gtaaaattac agtctggcaa gcattaggga tggatgcact actatctttc
    11161 atctggcctg tgttatctag caaaaaagaa tctgaagagg attattcgta tgcggtaaag
    11221 agcagtattt caaaaatcat tacatgtgca tttttgatat ggttagctag tttgtttatt
    11281 taaggagtgt tagcatgata ccaaaattta gagggttatc cattgacgaa aaaagcaaag
    11341 gagaatggca atacggacat ttaattgaag atagaggaag agcatttatt atcaacgaag
    11401 tggtagaagc caatgaacaa tacattacta taggttcttg gtgtcctgta aatccagcaa
    11461 cattaggaca atcaacagga ctcagagaca agaacggcaa ggaggtattc atcggtgaca
    11521 tcgttaaatg tacaagagga tgtctccatg aagtatattt agaaaaagaa tacggtggca
    11581 cattcatagg cggaatgcct tccatatatc taaagggatt gctaagtggg tatgcgtgga
    11641 ctgaagacga ggaaatcatc ggcaatgtct acgaaaatct agtttacatt ttttaaaaac
    11701 ttggaggtgg aaattgaaaa aattgagcga cgaagacctc aaaacattag acagagaact
    11761 tttcaaattt caaaacattc aacgtacaat agatttgaga aggctagaat tagaaactcg
    11821 aaacccagat gctcagagta gtcccagcgt aggaataagc aaacctaccg aaactatcgc
    11881 agtcagaatc gcagatgatc caaccttaaa atttctcgaa gggttcaaag ctatcattaa
    11941 caaactcttg atcaatctag ttgatgaaga taaggaaatc tttaatctgc gctggagata
    12001 tcctcaactg agatgggaag aaatagcaga acagaaattc atgagcaaag ctacaatcta
    12061 tcgacgtaga aggattatct tagaacagta cgctatactg aaaggtgagt tgtaaataag
    12121 attgagacaa aagacatctt gaagtctcat aaaaaaaggt ctattatgat agcatgaact
    12181 tctgaaacaa aaacacacat cacacttgag gagtcatcct taattctagt ccaaaagttg
    12241 tccaacagaa gtatcgtcaa gagtcagcaa atgctggctt tttgtttttg gaaaggaggt
    12301 agaatatgga atttgtatca ccgataaaag ataatgacga cattcaggca atgaaagatt
    12361 atcttagaga gtggaatgag atgtattata tgctattcat tacaggcctg aatactggtt
    12421 tgcgagtcgg agatatactt accttgaaag ttaaagatgt tcaaggctgg cacatcaaac
    12481 tgagagaacg gaagactggc aagcagataa caagacggat gacaaaagaa ctcaagaaag
    12541 aaatgaggag atatgttgag ggcaaaccat ttcatcattt cttattcaag agtaggcaag
    12601 gtcagaataa agcaatcact cgtgagcgag cctatcaaat catacatgaa gcagctgaag
    12661 aacttggcat tgataatgtt ggcacacata caatgcgcaa gacattcggc tataaatatt
    12721 acaacaagac aaaggacgta gggacattac agaaaatgtt caatcactca tcacctgcaa
    12781 ttaccctgag atacataggg atagaacaag cagagctcga tgatgcttta cggaactttg
    12841 tcatttaatt ttttagatat tactttcaca taatgagtta agcataaact gaaaaaatga
    12901 aactctttaa aacccatgct tagtaagggt ttgagattta gagtgagttt aacaaaatat
    12961 aagatatgtg aaagtgaggg ataaaattgg tatagttaca agaggtgaaa aatggtaaaa
    13021 gaataccgta tttgtaaatg gaatgcatat accgttaaca gccatactag taaaatggaa
    13081 taattttttt aaagaatcag aatgagacaa aagacatctt gaagtctcac aaaaaaaggt
    13141 ttattatggt agcatagatt tcttgtatga ggaggggata ggtcaaaggc ctgtcccttt
    13201 tagcattgag aaaggaggtt tgagatgtat aacaaaccta tcagaccatc cttgaaatct
    13261 aagaagtggg agaagttccg tgataggata atgcgtaagc atgattatct ttgccaagaa
    13321 agtttgcgtt acggaatttc tgttcaagca gaaatggtac atcatatctt tcctgtgtct
    13381 gaatatcctg agcttgaatt tgttgaatgg aattgcttgc cattgacgaa taagaaacat
    13441 aatacgtttc atgatagagt gaacgataga gtaatcaacc aaggattgta ttggcagaaa
    13501 aaaagaaaaa aagaattttt aatcccccct ctttttgaaa aatcattttg gccagtaggg
    13561 taccggtgaa gggaactttt tccaagtcgg gggccttcaa acaaaaaggg ggtaaaaact
    13621 aagcgatttt gacgaaagga ggtagttttt ggctaaacca attacagcaa agtcgattaa
    13681 gtcaaaagtg gtcaagcaga tgaaagactt gggaacttat cgtaaagagt tcgaaatgat
    13741 cattgacatt tttgcaggta tgttatacca gtatcagaaa cttgctcaag attatgctga
    13801 tatgggttat ccagtaacag acacctacgt caataaggct ggtgctgaaa atgagcgcaa
    13861 agttccaatc ttgacagcga tggaaatttt gaggaaagat atactcagct actctaatca
    13921 gttgatgatg aatcctaagt cgctcggtga ggtagtagaa caagagggtg agtcagttct
    13981 tactgaggta ctgaagttca agaacgaaat caagaagaag cgagtgactg gcaatgggta
    14041 atcttgataa agctaaaaaa tacgctcaac acgtcttaac tcaccgagaa gaacattgcg
    14101 aagaaaacat ccttgctgct gaacgttttt tccgtgattt agaaaatcca acctttgaga
    14161 tggatgagga tatggtggat tttgttattc actttatcga gaacgtgata gttcatcagc
    14221 agggcgatga tatgtttgcg gtatctatcc gtaacaagcc attgcttttg caaccgtggc
    14281 aacatttcgt tgtagttaat ctgtttggtt tttactacaa gggtacgaat gagcgcaggt
    14341 tcaaagaagc gctcatcatg cttgctcgga agaatgggaa gacctcgttt actgctgcaa
    14401 tcgcacttgc ttatcagata ttagatacag atagcggttc caaatgctat atcgttgcta
    14461 actcagtcaa gcaagcgatg gaagcttttg gttttttaag attcaacgtt gaacgctgga
    14521 acgataagaa cattcgtatc aaggataata accaagaaca ctctatcacc gccaattttg
    14581 gtgatgaagg ttctttcttt atccaggctt tagcgaatga tgagagccgt ctggactctt
    14641 tgaacggaaa tgttatcatc ttggacgaag ctcacacaat gaggaacagt aagaaacatg
    14701 gtcttatgaa aaaaacaatg tcagcataca gaaacagtat gctttttgtt atctcaacgg
    14761 ctggggatat tcctaccggg ttccttgcta accgtctgaa atactgtcaa aaggtgctca
    14821 agcaattagt cactgatgat tcatttttca tcttcatctg caaggctaat caatctgctg
    14881 atggggacgt ggtgaactat ctggacgaga atatcctcaa gatggctaat ccgtcatggg
    14941 gtgtcacggt ttcgttcaag gctctcaagg aagaagcaga gcaggctatg aatgatcctc
    15001 agacaagaaa tgagtttttc aataagacct tgaatatctt cactaactct atgaacgctt
    15061 attttaatcc tgatgagttt attgcgtcgg atagttgcta cgattggagt ttagaagagc
    15121 tggcacgttt gcctattcgt tggtatggtg gtgcggactt gtcaagattg cacgacttaa
    15181 cagctgctgc tctctatggt gtctatcatg acggtgagaa agacgttgat atctgtatca
    15241 cacatgcttt cttccctcgg attaatgctc agaaaaaagc taatgatgac gggattccac
    15301 tctttgggtg gcagtctgac ggttggttga cgatgagcaa cactccgacc gtcctctatg
    15361 atgatatcgt caaatggttc atcaagatga gggagaaagg gttcaagatt gctgctgtcg
    15421 gaatggatag gaagtttggc cgtgagttct tgacgaagat gaaacaagct cggttcaaga
    15481 tgattgacca acctcagctt ttttatctga aatcagaggg attcagacgg attgagttta
    15541 aagttaagaa taaagaattt tactatcttc attctgatgc ttacgaatac tgtgtgagca
    15601 atgttagagc aattgaaaag gtggacgatg ctgtgcaata tgagaaatta gatggagacg
    15661 gtgggactgc aaggattgac ttgtttgatg ccagcgtctt tgcttgtata caggctcttg
    15721 ctaatcttgg caagggtggt gatgtgatga gattctttga ttaggtgaat tatgaatgaa
    15781 atagtattat cagaacatga aattaacttg ctgatcaaca aagggcgagt taaagtaatt
    15841 ttaaacgggg aagtagtaac tattcgtcaa agacatatga aaaatttgat ggctgaaaca
    15901 gtaaaatggg aaaaacaggt aattgatgtc agtcagaata tcgtaagaaa taaacacttt
    15961 gattcacttt ttcaaaatac ttttcgttag aaaggaggtg aggaaacatg ggtatttttg
    16021 aaaagttttg gaaacgaaac aagccaagta agtcaatcaa catgctgagt cattcagatt
    16081 tagggttgtc aaacctgatg gactcgtatg tacctttggc cagaaatcca gatgtggtga
    16141 cagcggttaa taagattgct gatttggtct ctaatatgac catccacttg atggagaata
    16201 cagataaagg tgatatcaga atccgtgatg ggcttgctag aaagattgat atcaatccgt
    16261 gtaaacacat gacaaggaag tcatggattt tcaagattgt gcgcgatttg cttttatatg
    16321 gcgatgggaa ctctgtccta catgtggaat atgaacctgt tacggattat atttctaatc
    16381 taagaccatt tccgatgaga gaggtttcat tccaaacaga taaggattcc tatgtaatct
    16441 catttagggg tgaagaatat tcccctgatg aagtagtcca cttcgtcatc aatccagatc
    16501 cagatattcc atacattggt actggtttta gggtgacgtt gacagatgtg gttcaaagtt
    16561 tgaacatggc taccaagact aaaaaaagct ttatgaacgg gaagaatatt cctagtctta
    16621 tcgttaaagt agactcgtct agtgctgaac tagattcgga gcaagggcgt gagcgtatcg
    16681 ctgagaagta tttgagtact agcagggttg gcgctccatg gattgttcca gaggcattgc
    16741 tggacattca gcaggtaaaa ccgcttagtc taacggacat cgctttaaac gagtctgtgg
    16801 aattggataa aagaacagtt gcaggtctat taggagtacc tgcttttatt ttgggtgtag
    16861 gagagttcaa caagacagag tataacaact ttgtaaatac gactgtcatg agtattgcta
    16921 ccactattac tcaaacacta accagggact tacttttgtc tagcaatcgt tacttcaagc
    16981 taaatcctcg ttcactcttc tcttacaaca ttacggagtt gtctgctgtt gctcaacaaa
    17041 tggcaaacag tgctgcaatg cgtcgtaatg agtggaggga ttggctaggg atggctcctg
    17101 atcctgagat ggaagagttg attgttcttg aaaactttct ccctcaggag aaactaggag
    17161 atcaaaataa actgaaggga ggtgaggaag agaatgcaaa agcgaaatag ctatcgtgcc
    17221 actcaatttc aaacgagaga agaagaatct ggagacttgg tgttgagtgg ctattttata
    17281 aaatttgatg aagagacgga gttgtggcgt ggttatcatg aagtaatcaa gcgtgctggt
    17341 gttgagaaag ctgtcacaga cgctgatatc agagctttat ttaaccatga tgatagcctt
    17401 gttctcggtc gaacaggtaa cggaactctg acactgggtg ttgatgatgt tggtcttttt
    17461 ggagatatca tcattaacaa ggatgatcct caagctgttg gggcctatgc ccgtgtcaag
    17521 cgtggagatg ttatagggtg tagctttggc tttatcccgg taaaaatcga aacggaagaa
    17581 cgtgaagatg gttcatattt ggacactgtc ttagagctag aaatctttga agtgagtcca
    17641 tgtactttcc cagcctatcc acaaacggaa attgctgcac gacaaaaaga cttcgaaagt
    17701 cagaagcgtg ctaatcgtga agcgctagac aagcgcaaga aagaaattaa ggagaaattt
    17761 aagctatgaa taaggcatta atctttggtg ctcgtatgcg agcaaaagca actaaggtag
    17821 ttgagttgga agaaactatc gaagaattga acaaacgttc ggttgttgag ttagaaaagt
    17881 tggatcgtgc taaaaatgat gaagaagttt tagcagttga aaagactgta gacggtcttc
    17941 aaagggaaat tgaagaaaaa gaagctgaaa aagtacagct tgaaaacgag atcgatgagt
    18001 tagataaaca aatcaaagag caaaatcgta aagcaccaac tccggggaaa atggaagaac
    18061 gaggaggaaa gacattggga caacgtgaag catttaatca ttacttacga acaaaagaag
    18121 cgcgtgctga tggtttcaaa tctgccgaag gggaagcaat cattcctgtt gaattgatga
    18181 cgcctaaaga agcgaaacaa gacaagacag atttgacttc attggtcaac atcgttaatg
    18241 tcaagaacgc aagcggtaaa tggtcagttg taaaattgac tgatcaagaa atgaacactg
    18301 ttgaagagtt ggaagaaaac cctgaattgg ctaaaccaac ctttacaaag gtgaactatg
    18361 aaatccaaac acgtcgtggt catttgccag tatctcaaga atttattgat gacgctgact
    18421 acgatgtcat gggattggtt gctaaacaaa ctaagaacca agaacgtatc actaagaata
    18481 aagagatcgc taaagttctc aagacagcta catctaaaag cgcagctggt ttggacggct
    18541 tgaaagatat tctcaacgtg gaattgaaaa catactacaa tgcaactatc gtatgtactc
    18601 aatctatgtt cgctgctctt gataaaatca aggacaagga cggtcgctac atgctccaaa
    18661 cagatatcac atctccaact ggctacaagt ttgctggtcg tgttattgtt gtttatccag
    18721 atgatatcat tggagaaagc aagggagatt tgaaagcctt catcggtgac gttggagaat
    18781 ttgcgacatt gtttgaccgt gcgcagacta ctgtcaaatg gcaagatgac aaaatctacg
    18841 gtcagtattt ggcaaccgct aatcgttttg atgtagtaaa agtagacgga gatgcaggat
    18901 tttatgtgac ctatacagat gctgttttgt aaggaggtag taaatggtat ataaggtaat
    18961 ccgtcctttt aaggacttac gagacccaaa gaaacattgc tataaggttg gggataatta
    19021 ccctagagtt aagcacaaac ttgataaaga gtttgcggag actctgctaa atggggcaaa
    19081 tagtgcagga tctatctttt tgatgaatgt agaagataag gatatgtcga aagatgacac
    19141 attaactaca gaagtagaaa aggaagaaac aaaggtagta gatgggaaac ctgaagaggg
    19201 agacgaggag tagttatgga caatgttcaa ttattagaat tgcttaagct aaaattgggt
    19261 atagcaacaa aactacgcga taagcccttg gagaaaatca tcgaagctgt caaaacagaa
    19321 ctagaagata acttaggagt tctacttgac ttagacagct cagaagacca aatgtttgta
    19381 gttgattttg cagcctttcg ctatgagggt ggggtggata tgccacgtca ccttcagtgg
    19441 cgactgcata atttacagat agcatcaaag aaagaggtca agaatgtgga atcatgaaat
    19501 cacactgatc tctaaggaag tcacaggtaa ggataaacta ctacaaccaa tctctgaaga
    19561 tgttgaagtt actctcctat gtcgcaaaaa gagggtcact cgctctgaat tttatcaggc
    19621 gaatcaggta gggcttaaac cgagcttggt cgttgagatt cgaaattttg agtatgagaa
    19681 tcaagagctt gcgaagttcg aaggcaagca atatcatatc ttgaaaacct atcctattga
    19741 ttctgaaatt ttagagttga ctttgtcaga gatgttgaaa tgagcttaac aagtgattta
    19801 gcgaatgaaa ttgcaaaggc aatggcagag tactctgctg aggtagaaga taagattgac
    19861 ctgattgctg aggacgttgt aaacgaagcc gttacggaat taaaagcgac tagtccaaaa
    19921 cgtcatggaa agtatgctag aaattggcgc ttcaagaaaa atgctaaggg gtcatacgtc
    19981 atctacaacg cagctccaac ctatcgttta actcacttac tagaaaatgg gcatgttttg
    20041 agaaatggcg gtcgtagtcg ggcatttcca catattaaac ctgttgagga gaaagttaaa
    20101 gaaaactttg agaagcgaat caaggagatt ggaaaatgaa gctatcagac tttgcagcta
    20161 ttttggaaca ggcaaacttg cctgtcactt atcgagcgtt taaaactggg aacgctcctg
    20221 acctacctta tctggtctat tatgaatcaa ttccagccat caatgcagct gacaacacgg
    20281 ttaatcatca gattaagagc gtgacagtag agctagcttt tgagagtaag gatgaagatt
    20341 tggaagaacg tctggaagag ctgtggacaa cccacgagct ctttttcgat gttcaagaag
    20401 aaacatttat cgagattgaa agactctatg tcaagtctta tacagtctat ctatactaag
    20461 gaggaatgac atgaatcaag aaaataaagt aacttttggt ttaaaaaatg ttcacgttgc
    20521 gccaattaaa tcaattggtg cagatggagt gattgcttac gatgaaattt tccgctttcc
    20581 tggagcaatg gaattgacat tggatccaag gggtgaatca ataccaatca aagcagacga
    20641 tatcgattat cacttcatga actcaaacga agggtatgaa gggaaattca aaatctctca
    20701 cattattgaa atgtttgcga ctaagatttt gggtgaaatc aaagatgctc agacgggtgt
    20761 tttgactgaa aaagctgatg cagaattcac atcatttgcc ttgatgtttg aattttcagg
    20821 ggacaagaat aaaacacgtc acgtccttta ctactgttca gcgagccgtc caggcaatgg
    20881 ttcaaaaacc aaaaatggta caaacgtcaa cgagcgtgaa cttggcttta aagcaagtcc
    20941 tcgccctctg gattcagttg ttaaacgttc tatcacatca gctgataaca aggaaattta
    21001 tgacaactgg tttaagaaag tgtatgaacc tactgcggtg gcagcttaag gagaaaatct
    21061 atgcgtaaaa tcgtttgggt tggcgttcag gagtatgagt tggggaccaa tggctatact
    21121 cctatcgcct acaagcaaca atttgggaaa gattattttc aagatttgtt ttcaatgttg
    21181 aaaaatcaat cattcatgaa tgaattgaac aagctggaaa ctgacaaaga gttgactgca
    21241 actgatattg acatttcgat gctgtcagat tttgatatga catttttcaa ccgtcttttt
    21301 tggacctttg ctaaatctgc aaatcctcac atcaagcctt atgaacaatt cttcatggaa
    21361 atggaagtct ttccgattca ggaagttggg cctgtattga tggaaatgct gaatgcgagc
    21421 atgacgacaa aaaagcacca gatgaatcag aatcagctag cgaagaaatc ttcacagtag
    21481 aatcctactt atcttgctgt aaagaaactg gtctttctat tgatgatcta aagcacattt
    21541 caatcggaat ggctctggat tatcagacgg attatgtgaa tctacggagc gaggataagg
    21601 gtggcgaacg gaaagccacg caagctgatt ttgacagttt ttaaagaaaa atgagtgctg
    21661 agagagcgat tctgagacca agttccttgg tctggctgca ttatcagtcg tagaagttct
    21721 ctcagcgctt ttctattttt tgagaaagga ggaaatatgg caggaaatat caaaggtatc
    21781 aaaattgaaa tcgatggcga cacgcaaccc ttgcagaagg cgctgaaaaa tgtcaataag
    21841 gccgctactg atgcaagtca ggagttgaga cagattgaca aagccttgaa atttgataca
    21901 gggaacgtaa cgctcctgac tcagaaacaa gaggtcttac aaaagcaagt ttcgacgacc
    21961 aaagagaaac tagaaacctt gagacaagct cagtctcagg tggaacagca attcaaaaat
    22021 ggcgatatcg gtgctgacca gtaccgtgct ttccaacgtg aagtagaagt tactcaaaac
    22081 gtcctaaaag gatatgaggg taagcttgca aatgtgaatc aggcgcttaa tgagaatggg
    22141 agtgctactc agaacaacaa gaaccaatta aaagagttgc aaaatgagca gagtcaactt
    22201 gcttcagaga tggtaaaggt gacaagctca ttcaaactgc aagaaagcgc tttgggttca
    22261 aatgctagtg aagccgagag aaatgctctt gctcagaaaa agattggtgt tcagtctgag
    22321 attgtaagta aacagatttc aaatctagaa cagcaattgg aaatcactaa aaaagaattt
    22381 ggtgagaact ccacacaagc caacaggatg gaagctgagc taaatcaggc taagacagca
    22441 ttcaatcatc tcaatgatga gatgaaagga acaaagtctg ctgctgatag cactcaagaa
    22501 agtttaagtg aaatctcaag aaatttaaga gcagaactac ttcaacagtt tagtgagaag
    22561 ttgagtgcta tttcagaaaa gcttgtggaa gtaggaaaag aagcgttaga agcagctgct
    22621 caaatgcaag ctagtaatgc tcaatttact accgttttcg gagatatgga aacccaagca
    22681 agagaagcgt tgaatgctat tggtcaggaa atggatattg ttccagagcg attgcaaggt
    22741 tcgtttacac agatggcttc atttgccaaa acttcaggct tggacaccgc tgaagcattg
    22801 gatcttactt ctcgtgcaac tagggcagct gcagatggtg cagcctttta tgacaagtct
    22861 attgagagcg tgacagaaag cctacaatct tttttgaagg gaaactttgc taacgatgcg
    22921 gctcttggta tctctgcgac agagacaact aggaatgccg ctgcaaatga attgtacgga
    22981 aagtcattca aggacttgag cgaagcgcag aagcaattga ccttgcttca gatggtcgaa
    23041 gacggaaata aactctcagg agctcttgga caggctgcaa gagaatcaga cggcctagaa
    23101 aacgtgatgg ggaatctgaa acaagctggg accaatgcat tatctgctat tggtcaacct
    23161 cttctagaaa tgatgattcc tgtttttcaa accttggcaa cgattgtgaa aggtgtggcc
    23221 gagctgttca attccttgcc tgatccagta aaagatttca ttgtcatctt aggtgtggct
    23281 ttgacaattg taggagcctt agcccccata ttcttaaccc tgcaagctgt gtttatgtcc
    23341 tcatttggcg caatgattgc agcagcatta ccaatcattg gaattatttc aggagttgta
    23401 gtggccatag cagcgattgt tgctattgtg aaatatctct gggaaactaa cgaaggtttt
    23461 cgagatgcgg tcacgaccgt ttggaatgcg attcttgaag ttatcaatgc agtcgtatca
    23521 gagatttcta attttgtcat gagtatcttt ggaacggttg ttgcttggtg gacggagaac
    23581 caggaactta ttcgagcaag tgctgagact gtctggaatg ctatctatac ggttataagc
    23641 acaattctgg aaattttagg tccactcatt caaactggtt gggataatat ccaacttgtc
    23701 attacaacag cttgggaaat cattaagact gttgttgaga ctgcaataaa cgttgtcctt
    23761 ggtatcattc aagcagttat gcagatcatc aatggtgatt ggtcaggcgc ttgggaaact
    23821 attaaggggg tattctctac tgtatggcaa gctatccaaa gcattgtcca gaccattttt
    23881 tcagctatcc agagctacat ttcaaatgtt ctcaatggta tttcaggaac tgtatcaaat
    23941 atctggaacg gcatcaagga tactgtctca aatgtgttaa atggtatatc tggcacagta
    24001 tcaagtgttt gggaaggtat taagagtacc atctcaggag ctatcaatgg tgcaagggat
    24061 gctgtatctt cagctattga agctatcaaa ggattgttta actttagtat cagctggcca
    24121 catatcccac tacctcactt ttacgtgagt ggttcggcca atccattaga ttggttgagt
    24181 caaggtgttc caagtattgg aatcgaatgg tatgccaaag gcggtatcat gacgaaacca
    24241 actatctttg gaatgaatgg taataacata atggttggtg gtgaagctgg gaatgaagca
    24301 gtattgccac ttaacgacaa aacgcttgga gccatcggtc gaggtatcgc tcaaactatg
    24361 ggtggaactt caccaaccat taacattacc attactggca acactgtcag agaagaagcc
    24421 gacatcagtc gtattgctga tgaggtggct cagcgtattg ctgacgagtt gcaacgtaag
    24481 acacaattga gaggagggtt tacatgataa agcataatga gcttgtgatt gacggtgtga
    24541 gaacatcgtc ttttccgttt aaagtcattg tccatgattc tccttcaatt gctctgggag
    24601 agagcaagac agctcttttg gagcatggtg gtatcagtgg agcaatcgtt cagacgaaca
    24661 aacataggga attggtcaag aaaacctata cgatttactt ggtcaaacct actgaagaac
    24721 agatgaacca atttatgagt ctgtttatcc gtgaaaagtt ctggctagag agtgagcgag
    24781 tcaaaacaac tcgtctttgg tgctataagg tcaatgtgag cgaccttgaa gaagtgcaac
    24841 ctggtcttta catgaccaaa gcaaccttca cttgccatcc tacaaaatac tttaaaggct
    24901 ccgatacaca gagattgaca agaagtggaa ccttgaccgt tcaaggttct gctcttgctt
    24961 ttcctaaaat cacaatcgtt ggtcagagcg ctgttgagac ttcatttaca atcgctggtc
    25021 aggtcattag gcttgaaaaa ctctcagaat cgcttgtgat ggttaataat cctgacaatc
    25081 ctagctttaa aacgacaaca gggaagccag tcaaatggtc aggggatttt atcacagttg
    25141 atccagcgaa agttaagaat attggggttg ttttaggccc aggtattcaa tcgattgaaa
    25201 tcgaaacggt ttgggggtgg gcataattgc tttatctact taataaagat gtaagaaccg
    25261 ttcgatggaa cggggagcca cttcatgaag cgacttcggc gattgttaaa gagatcatga
    25321 atggtgattt caccttaact gtgaaatatc ccatttccga ctctggtatt tatcagctca
    25381 tccaagaaga tatgttgata aaagcgccga ctcctgttct tggtgcgcag ctatttcgca
    25441 ttaagaaacc tgttgaatac aatgatcatc tggaaatcac agcctatcac atttcagacg
    25501 atgtgatgca acgttctatc acaccagtaa gtgtgactag tcagagttgt ggcatggctc
    25561 tttctcgcat ggtacaaaac accaaaacgg ctcttggaga cttctcattc aacagtgata
    25621 tccaggaccg taggaccttc aatacgactg aaacagaaac cctatactct atattgctgg
    25681 acggtaagca cagcattgtt ggtacatggg aaggcgagct ggttcgtgat aactttgcga
    25741 taactgtcaa gaagagtcgt ggggagaatc gtggtgttgt tattacaacg cacaaaaatc
    25801 tgaagaacta ccaacgcaca aaaaacagtc agaatgttgt cacaagaatt cacgcaaagt
    25861 cgacttttaa acctgaaggt gctgaaaaag aaacgactat cagagtgact gttgatagtc
    25921 ctcttatcaa ctcataccct tatatcaatg aaaaagagta tgagaacaac aatgctaaaa
    25981 ctgttgaaga gttacagaag tgggcacagt ctaagttctc aaatgaggga attgacaagg
    26041 tctctgatgc tatcaagatt gaagcttatg aacttgatgg gcaagtggtc catatgggcg
    26101 acacggtcaa tctcaagagc tggaaacata atgtcgatgc attcaagaaa gctattgctt
    26161 atgagttcga tgccttaaaa gaagaataca tctctctgac tttcgatgat aaggcaggta
    26221 ttggtggttc tagagcttct ggtggcctat ctagcgcagc cgatgcaatt cttggagtga
    26281 cagaatctgc acaagaaatc gcccttgaaa aggctcttca aaatgctgac ttagactttg
    26341 atcataaggc tggattgctt agacaggaaa tttctgacga tattgaactt gccaaggcca
    26401 aggcggaaga agtcaagcga gaactgtctg acactatcaa tcagcgcttt aatagctttg
    26461 acaacgggcc attgaaagaa actaagcgca aggctgagga agctttgaga caagctggcg
    26521 caagtagctc tcttgctcag gaagccaagc ggattgggct ggattctgtt gctagacttg
    26581 aagcgtttaa gtcgcagact acgagcgcac aaacggctct gtcaggtgac ttggacgctc
    26641 tgaaacggac tatcgtgaat gatattcgac cgaagcaagc acaggttgaa gctgagattg
    26701 ccaagcaagt tgaagcactt gttcagacca aaaa
//
LOCUS       DBERDK010000022        25531 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.022, whole genome shotgun sequence.
ACCESSION   DBERDK010000022 DBERDK010000000
VERSION     DBERDK010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 25531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25531
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.022"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            158..625
                     /locus_tag="ACL73L_000948"
     CDS             158..625
                     /locus_tag="ACL73L_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771947.1"
                     /translation="MIDKVVRNLLLTFFFCKMTKIINFLTTIIVKKKKMCYNEFKLRN
                     RKQKGVIMWVLVFILFMIFFYSNNSKKIKKLENKIKKLERKEKGNAEMSRLLQEMIGK
                     EPIITGVYIGPDNWEVVDVDEEWVKLRSVDKKGKEKFKLQRIEDIQTVEFDGE"
     gene            635..841
                     /locus_tag="ACL73L_000949"
     CDS             635..841
                     /locus_tag="ACL73L_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGS0771948.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            841..1038
                     /locus_tag="ACL73L_000950"
     CDS             841..1038
                     /locus_tag="ACL73L_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771949.1"
                     /translation="MNKYKVIYYVVVIALLVSVFLLIGMDLSWFNPYQSDQFVWVYFA
                     LIPVTEWIEKKSKNLASEKGE"
     gene            1041..1775
                     /locus_tag="ACL73L_000951"
     CDS             1041..1775
                     /locus_tag="ACL73L_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HGS0771950.1"
                     /translation="MKKKRRLLFLMSIVLGGFLGMFVGMFKARVESHEIILDVKALMP
                     WISAICLLIGFISMFLTFNFLKKSRKFHSLYQEEMDDDLNETYYVQMYRNLEFGTIAF
                     NITGVAIPLAIFISLSEVIILHTNPQTFFLSFLLFVVFLVAQKSLFKTIAIVRQFDLE
                     FFATPKDVLNYINSYDEGERQANLEQSFRILFQLHQYVLPALYIFLIILSFLTGEIQL
                     LAFLLVGAIHVYINVMQLPMVKRYFK"
     gene            1787..2428
                     /locus_tag="ACL73L_000952"
     CDS             1787..2428
                     /locus_tag="ACL73L_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HGS0771951.1"
                     /translation="MKLLKNLGWFLLALLSFFFIYGFIQGLATASLALGASPYAVTLL
                     YVVLAGVYVYGIYKWYQKAPVYIEKSGFNRFIWLPALVWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRPLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYISRKGLVYSISLHALNNLVGFLMILML"
     gene            2446..2676
                     /locus_tag="ACL73L_000953"
     CDS             2446..2676
                     /locus_tag="ACL73L_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771952.1"
                     /translation="MKRVILLAVIQAVVLFFIIGALAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
     gene            2718..2921
                     /locus_tag="ACL73L_000954"
     CDS             2718..2921
                     /locus_tag="ACL73L_000954"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771953.1"
                     /translation="MKIKEQTRKLTAGCSKHCFEVVDETDEVSSKHGFEIVDETDEVS
                     SKHGFEVVDETDEVSNRTTVRRR"
     gene            3072..4835
                     /gene="aspS"
                     /locus_tag="ACL73L_000955"
     CDS             3072..4835
                     /gene="aspS"
                     /locus_tag="ACL73L_000955"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HGS0771954.1"
                     /translation="MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAAREQANDKLPTGAVELNVTALIV
                     LNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEKEIQDITEGLIARVMKETKGIEVTLPFPRMKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVKGVDFKVFSEAPAVKAIVVKGAADNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALALEDKDLVLFVADT
                     LEVANATLGALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EETAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSAEEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETSKTN"
     gene            4813..5754
                     /locus_tag="ACL73L_000956"
     CDS             4813..5754
                     /locus_tag="ACL73L_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HGS0771955.1"
                     /translation="MKQAKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLSFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINALFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
     gene            complement(5796..5948)
                     /locus_tag="ACL73L_000957"
     CDS             complement(5796..5948)
                     /locus_tag="ACL73L_000957"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771956.1"
                     /translation="MKIKVQTRKLAAGCSKHCFEVVDETDEVSSKHGFEVADRTDEVS
                     NIYTAR"
     gene            complement(6090..7076)
                     /locus_tag="ACL73L_000958"
     CDS             complement(6090..7076)
                     /locus_tag="ACL73L_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGS0771957.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
     gene            complement(7086..7886)
                     /locus_tag="ACL73L_000959"
     CDS             complement(7086..7886)
                     /locus_tag="ACL73L_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HGS0771958.1"
                     /translation="MLPYPFSFFSSIWGFRKPLSKRFELNWFQLLFTSIFLISLSMVP
                     IAIQNSSQETYPLETFIDNVYEPLTDKVVQDLSEHATIVDGTLTYTGTVSQASSIVIG
                     PSQIKELPKDLQLHFDTNELVISKESKELTRISYRAIQTESFKSKDSLTQAISKDWYQ
                     QNRVYISLFLVLGASFLFGLNFFIVSLGASLLLYITKKSRLFSFRTFKECYHFILNCL
                     GLPTLITLILGLFGQNMTTLITVQNILFVLYLVTIFYKTHFRDPNYHK"
     gene            complement(8139..8981)
                     /locus_tag="ACL73L_000960"
     CDS             complement(8139..8981)
                     /locus_tag="ACL73L_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HGS0771959.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            complement(8983..10275)
                     /locus_tag="ACL73L_000961"
     CDS             complement(8983..10275)
                     /locus_tag="ACL73L_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HGS0771960.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLGVTIVFVLYFL
                     ALATPELSNLITLGDKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            complement(10386..11657)
                     /locus_tag="ACL73L_000962"
     CDS             complement(10386..11657)
                     /locus_tag="ACL73L_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HGS0771961.1"
                     /translation="MSSKFMKSTAVLGTATLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKVTLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIAGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKDAKTAANDAVTLIKETIKQKFGE"
     gene            12192..13724
                     /gene="malQ"
                     /locus_tag="ACL73L_000963"
                     /pseudo
     CDS             12192..13724
                     /gene="malQ"
                     /locus_tag="ACL73L_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
     gene            13750..16008
                     /gene="glgP"
                     /locus_tag="ACL73L_000964"
     CDS             13750..16008
                     /gene="glgP"
                     /locus_tag="ACL73L_000964"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HGS0771962.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLGLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVRSSR
                     SYQVPFADFTLTSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKTDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTARGIELDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAAVKEKLESIKAHNKRKLARHLKEHQGVEINSNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLQVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGEENIYIFGENSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERLYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(17053..17625)
                     /locus_tag="ACL73L_000965"
     CDS             complement(17053..17625)
                     /locus_tag="ACL73L_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771963.1"
                     /translation="MNKLMKFISVFLTSIVLIVSAIPSVSAVYASEQVSQIETNMELQ
                     PVTSLTEEQINTLANEIQSFHPDVSQQWIKEVINRQLQGDYTIPPTYSPFRAAWQGIT
                     VNQMGALLDTAIALALGGTTAGLANLIKVKGKHAAKSAIRSAISRYLGSWFVNDVALE
                     SAMNLLSPGTYLAQLWDKNDAIPNNGRINF"
     gene            complement(18248..19096)
                     /locus_tag="ACL73L_000966"
     CDS             complement(18248..19096)
                     /locus_tag="ACL73L_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative RNA methyltransferase"
                     /protein_id="HGS0771964.1"
                     /translation="MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDL
                     AKFGYVNLAPQIKQSANYDKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAN
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTESHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQTASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSQLTEITIEAEI
                     LVGKAF"
     gene            19248..19526
                     /locus_tag="ACL73L_000967"
     CDS             19248..19526
                     /locus_tag="ACL73L_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6110 family protein"
                     /protein_id="HGS0771965.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKILASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            19528..21591
                     /locus_tag="ACL73L_000968"
     CDS             19528..21591
                     /locus_tag="ACL73L_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HGS0771966.1"
                     /translation="MSFKVLHRGYQHIRLSSSFSLTLDIQDYLRSLARDEKGIESIQF
                     YMDQQHFTLRIKEGFSVLDNAEAFLKRIDKGKVSELMTLPIRREESAYSIVSGAAVKR
                     VLFRSFVPYPIRYIWTCYQALGYIREAYQTLARKELTMEVLDCSAILLSLFMNQSKTA
                     SNIMFMLDLGNHLDQWSLKKTTTDLEQSLLAKESDVFLVQGDTVVSIKSSDVQIGDVL
                     ILSQGNEILFDGQVVSGLGMVNESSLTGESFPVEKRESDLVCANTVLETGELRIRVTD
                     NQMNSRILQLIELMKKSEENKKTKQRYFIKMADKVVKYNFLGAGLTYLLTGSFSKAIS
                     FLLVDFSCALKISTPVAYLTVIKEGLNREMVIKDGDVLEKYLEVDTFLFDKTGTITTS
                     YPIVEKVLPFGDYSEEDILRISACLEEHIYHPIANAIVKQAEIEGIEHEEMHGKLQYI
                     ASKGIKSHIDGQPVLIGNYVLMQDEQIHISSEQNALIEEYKSHYNLLFLAYQNELIGM
                     FCIHTPLRKEAKTALDKLKAQGKKLILATGDTLIRTEELVKDLPFDQVYTDLKPDGKF
                     ELVEKLQKAGHTILMVGDGLNDSAALTLSDIGVVMNESADISKQMSDILLLDNRLDFF
                     QELDSLSSSLQTLIKKNIQDTVVVNSSLIGFGLFNWFSPSNLSILHNLTTLRIVLRSL
                     SIKNR"
     gene            complement(21669..22925)
                     /gene="tyrS"
                     /locus_tag="ACL73L_000969"
     CDS             complement(21669..22925)
                     /gene="tyrS"
                     /locus_tag="ACL73L_000969"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HGS0771967.1"
                     /translation="MHIFDELKERGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAVWLNPEKTSPYEMYQFWMNVMDADAVRFLKIFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEEAYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADEHNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQDLDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            23067..>25531
                     /gene="pbp1b"
                     /locus_tag="ACL73L_000970"
     CDS             23067..>25531
                     /gene="pbp1b"
                     /locus_tag="ACL73L_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP1B"
                     /protein_id="HGS0771968.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKS
                     AIFPAILLSIKALFNLLFVLGFLGGMLGVGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEI
                     LTTYLNVSPFGRNNKGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVTGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEATQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLREKGVDVKGYMEKMGYEIPEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNPTLANADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALDPSVVKSEVLKSTGQKPGKVS
                     VEGKEVEVTGSTVTSYWANKSGAPATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSSRARR"
BASE COUNT         7595 a         4617 c         5544 g         7775 t
ORIGIN      
        1 aagtcagctc aaaacatggt tttgaggttg tagatgaaac tgacgaagtc agctcaaaat
       61 actgttttga ggttgtagat agaactgacg aagtcagtaa ccatacctac ggcaaggtga
      121 agctgacgtg gtttgaagag attttagaag agtattaatg atagataaag tggtcaggaa
      181 cttactcctg acctttttct tttgcaaaat gacaaaaatc ataaactttt tgacaactat
      241 aattgtcaaa aagaaaaaga tgtgttacaa tgaattcaag ttaagaaata ggaagcagaa
      301 aggagttata atgtgggtac tagtatttat actatttatg attttctttt attctaataa
      361 ttctaaaaaa atcaagaaac tagagaataa aatcaaaaaa cttgagcgaa aagagaaagg
      421 aaacgcagaa atgtcgagat tattgcaaga aatgattgga aaggaaccaa ttataacggg
      481 agtgtatatt gggccagata actgggaagt tgtggatgtt gacgaggaat gggtcaaact
      541 acgtagtgtt gataaaaagg ggaaagaaaa attcaagttg caacgtattg aagatatcca
      601 aaccgttgaa tttgacggag agtaggtgtg taacatgcaa ctaaaaaatc gtctaaaaga
      661 gcttcgagct cgcgatggtc tcaatcaaac cgacctagcc aaactggcag gggtttccag
      721 acagaccatt agcctactag aacgggatga gtacacccca tccattatca ttgccctgaa
      781 aatatctcaa attttcaacg aaacagtcga atcggtattt cgtttggagg aggacgagtg
      841 atgaacaagt ataaagtgat ttattatgta gtggtcatag ctttattagt cagcgtattt
      901 ctactgattg ggatggacct aagctggttt aatccttatc aaagcgacca atttgtttgg
      961 gtctactttg ctctcatccc agtaactgaa tggattgaaa agaaatccaa aaatctagca
     1021 agtgaaaaag gagaatgaat atgaaaaaga aacgtagatt gttatttttg atgtctattg
     1081 tcttgggggg gttcttgggc atgtttgtag ggatgtttaa ggcacgtgtc gaatcccacg
     1141 aaattatttt agatgtaaaa gccttgatgc catggatatc agctatttgt ttactgatag
     1201 gtttcattag tatgtttttg actttcaatt tcttaaagaa aagcagaaaa tttcattcct
     1261 tgtatcaaga ggaaatggat gacgatctga atgaaaccta ttatgtgcaa atgtatcgga
     1321 atcttgagtt tggaaccatt gcttttaata ttacaggtgt agcgattcca ttggctattt
     1381 ttatttcatt aagtgaggtg attatattgc atacaaaccc tcaaacattt ttcctttctt
     1441 tcttactctt tgtggtattc ttagtcgctc aaaaatctct ttttaaaacc attgcgattg
     1501 ttcgtcagtt tgatttggaa tttttcgcta caccaaagga tgtcttgaac tatataaatt
     1561 cttatgatga aggggagcgt caggctaatt tggaacagag ttttcgaatt ttattccaat
     1621 tacaccaata tgtcttacca gccttatata tttttcttat tatcctttct ttcttgacag
     1681 gagagattca gttactagct ttcttgcttg taggagccat ccatgtttat atcaatgtga
     1741 tgcagttacc tatggtaaaa cgttatttca aataaaggag ttatctatga aattacttaa
     1801 aaaccttggc tggtttcttc tagcccttct atcctttttc tttatctatg gctttattca
     1861 ggggctcgcg actgcgtcgc ttgctttagg cgcttcaccc tatgctgtta ctctactcta
     1921 tgtggtcttg gctggagtat atgtgtacgg catttacaaa tggtatcaga aagctcctgt
     1981 ttatattgag aagagcggct ttaaccgatt tatttggctt cctgccttgg tttggttctt
     2041 atctctggtc gttcaattct tcttgccaga tgatccttca gtaaatcagc aaatagcgac
     2101 agacttgacc ttgtctcaac cacttttctc attctttgct gtggttattt ttgctccttt
     2161 gacggaagag attgttttta gagggatgtt agcacgctat ctctttccta agcaggacaa
     2221 tagtaaacga cccctgattt ttcttctggt atctagtctt ctatttgcct tgattcattt
     2281 tccaggtgat gtgcaacaat tttttgtcta ttttagcctt ggttttagtt tgggtttggc
     2341 ttacattagc agaaaaggtc tggtctacag tatttctctt cacgctttga ataatttagt
     2401 cggctttttg atgattctca tgctataata gagtcaggag gtcacatgaa acgagtaatt
     2461 ttattagcag tgattcaggc agtcgttcta tttttcatca ttggagcgct agcttatgcc
     2521 ttcaaaggcg atttcttcta taactatcta gcagttgtct ttgctcctat tgcaggtgta
     2581 ctgcgttttg ggacggctta cataacggaa attgtcttgc ctcgaaaggc agccgaaatc
     2641 gctgaaaagc gtaaagcagg caaaaattca aaataaaaga gtccggtgaa gttcatcgga
     2701 tttttgtatt atactcaatg aaaatcaaag agcaaactag gaagctaacc gcaggttgct
     2761 caaaacactg ttttgaggtt gtggatgaaa ctgacgaagt cagctcaaaa catggttttg
     2821 agattgtaga tgaaactgac gaagtcagct caaaacatgg ttttgaggtt gtagatgaaa
     2881 ctgacgaagt cagtaaccgt actacggtaa ggcgacgctg acgtggtttg aagagatttt
     2941 cgaagagtat tagcgctaat ttttagggct ttcacctgac ttttaaagac tggcaaactt
     3001 ttctgatgat tttcatgaag agcctgcgct tttatggtaa aatagtaaca gaataaaaga
     3061 ggagagaaat aatgaaacgt agtatgtatg ctggtcgtgt tcgtgaggaa cacatcggac
     3121 aagaaataac cttgaaagga tgggttggcc gtcgtcgtga ccttggtggt ttgatcttta
     3181 tcgatcttcg tgaccgtgaa ggaatcatgc agttggttat caaccctgaa aaagtatctg
     3241 cagaggttat ggcaacagct gaaagccttc gtagcgaatt tgttattgag gtgactggtc
     3301 aggtcgctgc gcgtgagcaa gccaatgata agttgccaac tggtgcggtt gagttaaacg
     3361 tgacagctct tattgtgctt aacacagcta agacaacacc atttgagatt aaggatggca
     3421 ttgaggcaaa tgacgataca cgtttgcgtt accgttacct tgaccttcgt cgtccagaaa
     3481 tgttggaaaa tcttaaactt cgtgccaagg tgacccactc tatccgcaac tacttggatg
     3541 agttggagtt tatcgacgtg gagacaccat tcctttctaa gtcaacgcct gaaggggcgc
     3601 gtgattattt agtgccgtct cgtgttaata aggggcattt ttacgctctt cctcaaagtc
     3661 cacaaatcac gaaacagctc ttgatgaatg ctggttttga ccgttactac caaatcgtta
     3721 aatgtttccg tgacgaggac ttgcgtggag accgccagcc tgaatttact caggtcgact
     3781 tggaaacgtc cttccttact gagaaagaaa tccaagatat tacagaaggc ttgatcgcgc
     3841 gcgtgatgaa ggaaacaaaa ggcatcgaag taacgctacc gttccctcgt atgaaatacg
     3901 atgatgctat ggctctttac ggttctgaca agccagatac tcgttttgac atgttgcttc
     3961 aggacttgac agaagtggtc aaaggtgtag actttaaagt cttttcagaa gcacctgctg
     4021 taaaagcgat tgtggtcaaa ggagctgcgg acaactattc acgtaaagac atcgacaaga
     4081 tgacggaagt agccaaacag tatggtgcca aaggtcttgc ttgggtcaag gtggttgatg
     4141 gagaattaaa cggaccagtt gccaagttct tgactggtat ccaagaagaa ttgacaacag
     4201 cgcttgctct tgaagataag gacttggttc tctttgtggc ggatacgctt gaagtggcta
     4261 atgcaacact gggtgccctt cgtggacgta ttgctaaaga gcttggcttg attgataatg
     4321 ataagttcaa cttcctttgg gtggttgact ggccgatgtt tgaatggtct gaagaagaag
     4381 gccgctacat gagcgcccac catccattca cacttccaca ggaagagact gctcacgaat
     4441 tagaaggtga tttggctaag gttcgtgcca ttgcttacga tatcgtcttg aacggttatg
     4501 agcttggtgg tggtagcctt cgtatcaacc aaaaagacct tcaagaacgc atgttcaagg
     4561 ctcttggttt ctcagctgaa gaagccaatg accagtttgg tttccttctt gaagccatgg
     4621 actatggttt cccaccacac ggtggtttgg ctatcgggct tgaccgtttt gtcatgttgc
     4681 ttgctggaga agaaaatatc cgtgaagtca ttgcctttcc taagaacaac aaggcaactg
     4741 acccaatgac acaagctcca tcaacagtcg ctctcaaaca actagaggaa ctcagcttac
     4801 aagtagaaga agatgaaaca agcaaaacga attaagcggt ggcgctatta tctgcgccgc
     4861 tttgcttatc agataaaaat tttacgtgtc ttacaaagta tctctcgaga aaagtatgat
     4921 gagaagattt cggcctctct ggtctatggt tttttatcag cagtagcagt taatttcttt
     4981 ttccaaccag ggcatgtgta ttcgagtggt gcaacaggtc tggcacagat tatctctgcc
     5041 ttgagtaatc actggtttgg ttttcatatt ccgatttcgc taagcttcta cgccattaac
     5101 ttccctttga tggtcttagc ttggtatcag attggccata agttcaccgt ctttaccttt
     5161 atcacggtat ctatgagttc cttctttatc cagtttgtcc ctgtggcaac cttgacggag
     5221 gatcccatta tcaatgccct ttttgggggt gttgttatgg gtttggggat tggttttgct
     5281 cttcgaaaca atatctccag tggtgggacg gatatcgtca gcctgactat tcgcaagaaa
     5341 acgggtaaga atgtcggtag tatttctttc ttggtaaatg gaactatcat gctgatagca
     5401 ggtttgacct ttggttggaa atacgctctt tattctatga ttaccatctt tgtctctagt
     5461 cgtgtgacag acgcagtctt tactaagcaa aagcgtatgc aggccatgat tgtgacaaat
     5521 catccagaga aggtaattga aaaaatccat aaaaaattgc accgcggagc aaccatgatc
     5581 cacgatgcag aaggaaccta taatcacgag agaaaggcag ttttaatcac tgtcattaca
     5641 cgtgcagagt ttaatgaatt taaacagatt atgacacaag tggatccaag ctcctttgtc
     5701 tctgtctcgg aaaatgttca tattctagga agatttgttg agatagataa ttagtgatta
     5761 atactcttcg aaaatctctt caaacctcgt cagcttcacc ttgccgtata tatgttactg
     5821 acttcgtcag ttctatctgc aacctcaaaa ccatgttttg agctgacttc gtcagtttca
     5881 tctacaacct caaaacagtg ttttgagcaa cctgcggcta gcttcctagt ttgcactttg
     5941 attttcattg agtataaaaa gatcttggga aaagactcaa tttcagtaga aaaggaagta
     6001 aatcttccca caataaaacg catagtatca agttttttca atacctgata gtatgcgttt
     6061 ttgcttttaa cgactttttg cccagtctct tatttttcga taattttgtg ggcgatcaat
     6121 tgacggtaac aaatttgttt attgttttta ttatcattaa taatctggag aatagtttca
     6181 agggaaacac gaccaagctc taaactattg atatcgacat aggctgccaa gttgagcttg
     6241 ggattaaccg agtcaaagct gagaacaggg acatccagct ggtgtttggc aatatagtta
     6301 caaacacctt cagctaggag gctatcggtt gtgatgatag catcaatttg tggatcgtgc
     6361 ttgaataatc gcttgctaaa tttatagccc ttttcttcca gaaactcgtc ggcaaagtag
     6421 atgcgattgt tgtcagtggt aagtttgtaa tgtttaagcg cctgttcata gcctgttaaa
     6481 cggtctttgg tcacgaagag ctttttactt cctccgataa aggcaatgcg tttgcagcct
     6541 tttttgatga aatattcagt cgcatcaaaa ccagcttgaa cattgtcgtt gtcgacaagt
     6601 gggatgaaag gagatagaga tttacctaag ataaggaagg ggaactgttc ttctgcgacg
     6661 agttttacga gagggtcttc ttcttgggca tagagaaaga ttagcccatc tacacgcttg
     6721 ccgtagacca tttgtgaaat agcgttgaga cgctccttct catctttccc tgttgctatc
     6781 tgaatggcat agtggttttc agatgcgact tgagagatgc cacgtagaac cgatggaaag
     6841 aaaggattct ggtagaaggc gtctgagtca tcaggaagaa ctaatccgat aacctgagta
     6901 tagctgctta ccaagctacg agcgttgagg tttgggtggt agttgagttc cttcatagct
     6961 ttgcgaacgc gtttttttgt ttcgtcgcta atggttgatt tattttgaat aacacgggtt
     7021 acggttgaag gcgaaacacc agcagccttg gccacgtctt taatcgtaac gggcataaaa
     7081 atctcctatt tatggtaatt tggatcacgg aaatgtgttt tataaaagat agtgaccaga
     7141 tacagaacaa aaagaatatt ttgtacagta atcagggttg tcatattttg gccaaataat
     7201 cccaaaataa gtgtaatcag agtcggcaat cctaaacagt tcaagataaa gtgatagcac
     7261 tctttaaagg ttctaaatga aaagaggcgt gatttcttag tgatataaag gagaagacta
     7321 gctcctagag agacgataaa gaaattcaaa ccaaagagga agctcgcacc gagaactagg
     7381 aagaggctga tatagacacg attttgttgg taccagtctt tagaaattgc ttgggtcaag
     7441 ctgtctttgc ttttgaaact ctcagtctga atggctcggt aagagatgcg ggtcagttcc
     7501 ttgctttcct tgctgatgac tagctcattt gtatcgaaat gcagttgcaa gtccttaggt
     7561 aattccttga tttgacttgg accaatcaca atagaagagg cttgactaac tgttccagta
     7621 taagttaatg tgccatcgac aattgtagca tgttcagaga gatcctggac aaccttatct
     7681 gtcagaggtt catagacatt atcgataaaa gtttctagcg gataggtctc ctgggagctg
     7741 ttttggatag caatgggtac catagacaag ctgataagga agatactggt aaagagaagt
     7801 tgaaaccagt tgagctcgaa acgtttggac aggggcttac gaaaccccca aatacttgaa
     7861 aaaaaggaaa atggatatgg aagcataggt atctttctaa aaggtgtttt ctatataagt
     7921 attattatat agagaaatgc gctttatttc aagtctagga gaaaaattgc ttgctacaag
     7981 gatattattt acagtgacaa ttgctaataa tcaatgaaaa tcaaagtgca aactagccac
     8041 aggttgctca aagcacaact ttgaggttgg agataaagct gacgtggttt gaagagattt
     8101 tcgaagagta taaaatgaaa aagggtggcg gggataaatt atcccttgtc gccaccactt
     8161 gtaagtcctg aaacaaagtt cttttgtagg aagaagaaga gaatacagat tggaagggcg
     8221 atgaggatag cacctgctga gaagtaggca atcttcaagt ttttcgcatt gttaacgaag
     8281 gtttggagac ctacggcaac agtaaagtat tctttctcac gaagcaagaa actagagagg
     8341 atgtagtccc cgaaaggtcc catgaaggcc cagagagctt gtacggcaac cattgggcga
     8401 acaagtggta gaacaatttg ccagaagcgg cggaagtgtc ctgcaccgtc tagttttgca
     8461 gattcgtcta aagacattgg cactgtatcg aagtagcctt tcatgagcca agcattcatc
     8521 gggataccac caccaacgta gaggaagatg aggaaccagt tgtggttaag ggcgttcaac
     8581 ataagcgcca taacgaagaa ggctgtcaaa gcggccatag ttggcaccat ttggatgatc
     8641 aagaagaaga ccaaactttg tttacgagcc aagaagttgt aacggctgta agcataacca
     8701 gcaagtacga tgatacttgt ttgaacagcc atggtaatta aggcgataat caaagtgttg
     8761 aggtaccaag taccgtacaa ggtttcagtg aagaggcctt taaagttatc aaaattgagg
     8821 tcgatattag tatctagttt aaaggctgag acgttacctg ctttaaaggc tgacataatg
     8881 gtaatcaaca gtggatagat aattacaatt gatagaccaa tcaggtaaag gtaagtaagg
     8941 ctttgagtca gtctacgttt gagtttaatt gagttattca tcttagacgt cctccatatc
     9001 aaatgcgtgt agtttcttga atgcgatcat agagattgag atgacaatga tagagataat
     9061 caaggtaaca gctgccgcca ttgagtattg aggagatgta cctgttgtca aacggtagat
     9121 ccatgagatc aagatatcgg ttgaaccagc tccacctccg acactaccag gtcctccacc
     9181 attgaagagg tacatgatag agaagttgtt aaagttgaag gtgtattggc taatcaaagt
     9241 aggtgccgca acagccaaaa tcattgggaa agtgatgttg cggaattttt gccaagcgtt
     9301 ggcaccgtca atataagctg cttcgtaaag gtcgttagga atagattgca agatacccaa
     9361 agtcaaaacg tagatgtatg ggaatccgag ccaaccttgc atcataatca aggcaatctt
     9421 agtccaagtt gggtctgttt tccaaggaat aagagctcca tcaaggaaag gaaggaattt
     9481 agccaagatt ggcaatactt gagtgttgat agcaccgaca ctatcgttaa acatatttga
     9541 gaatgtcaag atagtgatga aggctgggac agcccaagga agaaggaaaa taacaccaaa
     9601 gatacgtttt cctttgataa atggttggtt ggcaatgata gctgtgaaga taccaattac
     9661 gatttgtaaa gtagaagctg ccaaagccca aatgatagtc caagaaagaa cagaaccaaa
     9721 ggcagaacgg aaggtactca agctccaaat gtttgtaaag ttggtcaaac caacccagtc
     9781 caacaacttg tttggtggca agtgttggaa gtcgtagttg gtaaaggcga tcatcaaggt
     9841 tacgataact gggaagataa tcgcgaaggt catggcaaca taagatggaa tgatcaagag
     9901 gtaagggaag ccattttcat aaatcccttt gatcatgtct ttgagtgtgc gtggaactgg
     9961 aattccattg ttaatgcgtt ttgcaatcgt atgtgcatct ttgatatttg agaaataaaa
    10021 gagtacataa acgattacaa agattagatg gaaggcacca cgaatcagca taaagaggga
    10081 attatcacga cctggtttgt caccaagagt gatgaggttg ctcaattcag gggttgcaag
    10141 tgctaggaag taaaggacaa atacgatagt tacaccaagg aagataaaac ctttggcttt
    10201 ttgtttattg taaatctgtc ctaacccagg aatgatagac agcagggctg ctttactagg
    10261 ttgttgcttt tccatattaa ctcctttcat aggatactac ttttttataa gaaagctaaa
    10321 ttatttgtgt ctctattgat aaattcaaag ggggatttga tttccacccc ccttgaacaa
    10381 attttttatt caccaaattt ttgtttgatt gtttctttga tcaatgttac agcatcgtta
    10441 gcagctgttt tagcatcttt ttgaccactt acagcatcaa agagcatatt tttcgctgga
    10501 tcccaaactg cagacatttg agagatgttt ggcagtggtt gagtgttctt gaactgtttg
    10561 ataacagctg ttgtcaactc atcgttttta ccttcagcgt atgaacgagc ctcagtatta
    10621 gctgggattt cgttagtctt atcatataat actttttgtt gttcagttgc aacaaggaag
    10681 tctacaaatt tttgagaagc ttcaaggttc ttaacggctt gaggaatgac ccaagcttta
    10741 ccaccaccga atgcagcata ttcttttcca tttggaagag ttgggatagt tgcaactccg
    10801 tagtttactt tagcatcttt aaaggcttga gctttccaag gtccgtcgat gatagcagct
    10861 gttttacctt cttggaattg agtttggatt aagtttccag caccttctgt atcttgcata
    10921 cctttaggcc atttttcgta ccaagattta gcgtagttga tacctgcgat agaaccgtcg
    10981 tttgcaagac cgatgtcttt agcgtcttta ccgttttggc caaagacgta agcaccgtta
    11041 ccggcaagaa gtccatatgt atagtagaag tttgtccagt cagctaggaa ggcagtagtt
    11101 ttaccatctt caccagcgaa tgcgtatttg ctatctttag caaggttttc caagtcagca
    11161 aatgtttttg gagcatcttt caccaagtct ttgttgtagt acataacaag tgactcgata
    11221 acggcaggag caccgtaaac tttaccatta gcagctgtta caagagattt agttgtgtcg
    11281 tctgttttag caccatcgct caatttcact tctgaaagtt gtccgtcaga accaaggcta
    11341 cctacacggt cgtatggagc catcataaca tcagggacat taccagattg gttgtcaaga
    11401 gaaagtttat caagacctcc tagagcatca ccagttttaa gagtgacttt tactccagct
    11461 tctttttcat aagctttagc aacctcttca atatagctct tatatccctc gtctacatat
    11521 acagtgagtt ctttgacttc agatgaacca gaatcagcag gcttatcagc agttttgctt
    11581 ccgcaagcta ccaaaagcaa gctagcaagt gtagcagttc caagcaccgc agtgctcttc
    11641 ataaatttag atgacatagt gtattcctcc caaagaatag caagttttat tgataaggaa
    11701 acgcaaacgt tttcctttat gagcttagta tagcacaaat agaaaacggt tgcaagtatt
    11761 ttttgtaaaa attttaaaaa atttttaagc ttgatttgat agcataattt tgcattttaa
    11821 tagaagaaaa gtatgaaata gataagaata cagtattttt aataaaaata tatggaatct
    11881 gtctaaagag ggaaatcagg aggggctacc tccctgtttc taatccctat ctattttcta
    11941 tagacgtttg tgctttgttg catataaatt caagctccct aaggaggttt tgtactttgt
    12001 agactgtctc gtgagagtca aactttagga ttagatagtt ttaagttatc tttgctaaaa
    12061 tgagttagtc tccttaaaaa taatcaaaaa agttttttaa aaacgcttgc aattatgctt
    12121 gaaaaggagt atacttataa gtaacgcaaa cgtttgcgtc tgcaaaatac gcaacgttcc
    12181 attattttaa cacacgaggt gctattatga aaaaacgtca aagtggtgtg ttgatgcaca
    12241 tctcttctct tccaggagct tacggaatcg gatcatttgg tcaaagtgct tacgacttcg
    12301 ttgatttctt ggtccgtaca aaacaacgtt actggcaaat ccttccatta ggagcaacta
    12361 gttacgggga ttctccttac caatctttct cagccttcgc aggaaacact cattttatcg
    12421 atttagatat cttggtggag caaggtttgt tggaagcaag tgaccttgaa ggagttgact
    12481 ttggtagcga tgcgtctgaa gttgactatg ctaaaatcta ctatgcacgt cgtcctcttt
    12541 tagaaaaagc ggtgaaacgt ttctttgaag tcggagatgt taaagatttt gagaaatttg
    12601 ctcaagacaa ccaatcatgg cttgagctct ttgctgagta tatggctatc aaagagtatt
    12661 ttgacaatct tgcttggact gaatggccag atgcagatgc tcgtgctcgt aaagcttcag
    12721 cacttgaaag ctatcgtgag caattggcag acaagttggt ttaccaccgt gtgactcaat
    12781 acttcttctt ccaacaatgg ttgaaattga aagcttacgc taacgacaac cacatcgaaa
    12841 tcgttgggga catgccaatc tacgtagcgg aagattcaag tgatatgtgg gcaaatccac
    12901 atctcttcaa aacagatgtc aatggtaagg ctacttgtat cgcaggatgc ccaccagatg
    12961 agttttctgt aactggtcag ctttggggta acccaatcta tgactgggaa gcaatggaca
    13021 aagacggcta caaatggtgg attgaacgct tgcgtgaaag cttcaaaatc tacgatatcg
    13081 ttcgtatcga ccacttccgt ggcttcgaat cttactggga aatccctgct ggttccgata
    13141 cagcagcacc tggtgagtgg gtgaaaggtc caggctacaa gctttttgca gccgttaagg
    13201 aagaacttgg tgagctaaac atcatcgcag aagaccttgg cttcatgaca gatgaagtga
    13261 tcgaattgcg tgaacgtact ggcttcccag gaatgaagat tcttcaattt gccttcaacc
    13321 cagaagacga aagcattgat agcccacact tggcacctgc taactcagtt atgtacacag
    13381 gaacacacga taacaatacg gttcttggtt ggtaccgtaa tgagattgat gatgcgactc
    13441 gtgagtacat ggctcgttac acgaaccgta aagaatacga aacagtggta cacgctatgc
    13501 ttcgtacagt attttcatca gttagcttta tggcaattgc aactatgcaa gatttactag
    13561 aattggatga ggcagctcgt atgaacttcc catctaccct tggtggaaac tggtcttggc
    13621 gtatgactga agatcaattg acaccagctg tcgaggaagg tttgcttgac ttgacaacaa
    13681 tttatcgccg aattaatgaa aatttggtag atttaaagaa ataagacaat aatcaggaga
    13741 caactaaaca tgttatcact acaagaattt gtacaaaatc gttacaataa aaccattgca
    13801 gaatgtagca atgaagagct ttaccttgct cttcttaact acagcaagct tgcaagcagc
    13861 caaaaaccag tcaacactgg taagaaaaaa gtttactaca tctcagctga gttcttgatt
    13921 ggtaaactct tgtcaaacaa cttgattaac cttggtcttt acgacgatgt taaaaaagaa
    13981 cttgcagctg caggtaaaga cttgatcgaa gttgaagaag ttgaattgga accatctctt
    14041 ggtaatggtg gtttgggacg tttggctgcc tgctttatcg actcaattgc tactcttggt
    14101 ttgaatggtg acggtgttgg tcttaactac cactttggtc ttttccaaca agttcttaaa
    14161 aacaaccaac aagaaacaat tccaaatgca tggttgacag agcaaaactg gttggttcgc
    14221 tcaagccgta gctaccaagt accatttgca gactttactt tgacatcaac tctttacgat
    14281 attgatgtta ctggttatga aacagcgact aaaaaccgct tgcgtttgtt tgacttggat
    14341 tcagttgatt cttctattat taaagatggt atcaactttg acaagacaga tatcgctcgc
    14401 aacttgactc tcttccttta cccagatgat agtgaccgtc aaggtgaatt gctccgtatc
    14461 ttccaacaat acttcatggt ttcaaacggt gcgcaattga tcatcgacga agcaatcgaa
    14521 aaaggaagca acttgcatga ccttgctgac tacgcagttg tccaaatcaa cgatactcac
    14581 ccatcaatgg tgattcctga attgattcgt cttttgactg cacgtggtat cgagcttgac
    14641 gaagcaatct caattgttcg tagcatgact gcctacacta accacacaat ccttgctgag
    14701 gcgcttgaaa aatggcctct tgaattcttg caagaagtgg ttcctcactt ggtaccaatc
    14761 atcgaagaat tggaccgtcg tgtgaaggca gagtacaaag atccagctgt tcaaatcatc
    14821 gatgagagcg gacgtgttca catggctcac atggatatcc actacggata cagtgttaac
    14881 ggggttgcag cacttcatac tgaaatcttg aaaaattctg agttgaaagc cttctacgac
    14941 ctttacccag aaaagttcaa caacaaaaca aacggtatca ctttccgtcg ttggcttatg
    15001 catgctaacc caagattgtc tcactacttg gatgagattc ttggagatgg ttggcaccat
    15061 gaagcagatg agcttgaaaa actgttgtct tatgaagaca aagcagctgt caaagaaaaa
    15121 ttggaaagca tcaaggctca caacaaacgt aaattggctc gtcacttgaa agaacaccaa
    15181 ggtgtggaaa tcaattcaaa ttctatcttt gatatccaaa tcaaacgtct tcacgagtac
    15241 aaacgccaac aaatgaacgc tttgtacgtg atccacaaat accttgacat caaagctggt
    15301 aacatccctg ctcgtccaat cacaatcttc tttggtggta aagcagctcc agcctacaca
    15361 atcgctcaag acattatcca tttaatcctt tgcatgtcag aagttattgc taacgatcca
    15421 gcagtagctc cacacttgca agtagttatg gttgaaaact acaacgttac tgcagcaagt
    15481 ttccttatcc cagcatgtga tatctcagaa caaatctcac ttgcttctaa agaagcttca
    15541 ggtactggta acatgaaatt catgttgaac ggagctttga cacttggtac tatggacggt
    15601 gctaacgtgg aaatcgctga gttggttgga gaagaaaaca tctacatctt cggtgaaaat
    15661 tcagaaactg ttatcgacct ttacgcaaaa gcagcttaca aatcaagcga attctacgct
    15721 cgtgaagcta tcaaaccatt ggttgacttc atcgttagtg atgcagttct tgcagctgga
    15781 aacaaagagc gcttggaacg tctttacaat gaattgatca acaaagactg gttcatgact
    15841 cttcttgatt tggaagacta catcaaagtc aaagagcaaa tgcttgctga ctacgaagac
    15901 cgtgacgcat ggttggataa agtcatcgtt aacatttcta aagcaggatt cttctcatct
    15961 gaccgtacaa tcgctcagta taacgaagac atctggcact tgaactaata ctcttcgaaa
    16021 atctcttcaa accacgtcag ctttatctgc aacctcaaag cggtgctttg agcaacctgc
    16081 ggctagcttc ctagtttgct ctttgatttt cattgagtat aagatacaaa tttatactaa
    16141 tacattttgt aaaaaagcga gtttcgattg aaattcgctt ttttaaatgc tgtggatttg
    16201 ggtcaatctt gtctaaaaat agggaaatcc tagatacagt gaaggtttta aatgctggtt
    16261 tttactgtct tcagccttat attttttcgt agttggttac ctcatatctc ttgtattcgc
    16321 ttacataaag tattataata taattgtagg aaagaaggtg tttttatgat atacacactt
    16381 aaattggtgt tgtttattac ctttcttgta ataagcttgt tacctgataa gatttttgga
    16441 aaaaataaaa aaatttggaa aatagttttt gcaatattga cggcagtggc agcattgtca
    16501 tttatgtact aagttatttt aagaatgtag ggaaataaac cctacattct ttttagtttt
    16561 ttctgttttc taaattctat ttatccaagc gcttcaacat ttcttgcttc ttcgcttcaa
    16621 gttctgcatg cttttcttcg atttcggcat gttttttctc gagttcagaa caacttgcac
    16681 cattgctaaa ttcttttcgc catcaggaga tagggtgagt cgacatgtct attactcacc
    16741 caaagcagtc ctacaaagca ggaattttct gttacttttt tggaaatagt aacgtttata
    16801 cagctttgac acttcgtatc aaagcgccaa acacactccg agggatttac agaaagcaga
    16861 aaaggaatga tctggtataa gatcattcct tttctctctt tttctttaag taattatata
    16921 caatgtacga cgaagtcgtc attgcaatgc tgatccacca cctaaaggga actttaaaca
    16981 acattgataa gataaagaat ataaacaacg aaaatacgtt atacccaatt aattttattg
    17041 tatatctcat gattaaaagt taatccttcc gttgttagga atggcatcat ttttatccca
    17101 taattgtgct aaataagtcc ccggtgataa taaattcata gcggattcta aagcaacatc
    17161 atttacaaac caactaccta gatatctaga aattgctgaa cgaatagcac tttttgctgc
    17221 atgttttcct tttactttaa ttagatttgc aaggcctgca gtagttcctc ctaatgctaa
    17281 agctattgca gtatctaata gagcacccat ttgattaact gtaatacctt gccaagctgc
    17341 tctaaatgga gagtatgtag gtgggattgt ataatcgcct tgtaattgtc ggttaattac
    17401 ttctttgatc cattgttgtg agacgtctgg atgaaaagat tggatttcgt ttgcaagtgt
    17461 attgatttgt tcttctgtta gagaagtgac aggttgaagt tccatatttg tttcaatttg
    17521 tgatacttgt tcagaagcgt atacagctga aacacttgga atcgctgata caattaacac
    17581 aattgacgtc aaaaaaaccg aaataaattt cattaatttg ttcatgagct tttctccttt
    17641 ttatttgtat ctgcttacat tttattatat actgttatta tagtcaagaa aaatatgcta
    17701 ttatgttaaa aaaatatttt taaaaatata aatggacgga tttattttgg attttatttg
    17761 ttattttgac ctgcctctat attggtaacc atgatttgtt tactttcaat catcaagaat
    17821 tctcttttcg tggtagcgtt tggggtctgg tactggcctt atatcactta ttgttcattg
    17881 ataagtttgt aatttcgaat cgaaaataaa gattagaact atgattgact ggctactttt
    17941 aatattgatt ttggaggaag attttgtctc tattatttat tattttaaat ttatctattt
    18001 tgtacaagat ctattctttg agacgggaga aatcgaaata cttgatttat acggcctata
    18061 tcatatttgg ggtaaatgta gtatatggta ttcaatggtt attaagagaa ctgatttcaa
    18121 ctatttcccc ttaatgtgta tggaaactat aacaaaacgc ataagattaa ggtttgtgta
    18181 cctgttctta tgcgtttttg taatattgaa gattttcggt aagttttata cctttgggaa
    18241 gtctagttta gaatgctttc ccaaccaaaa tctctgcttc gatggtaatc tctgtcagtt
    18301 ggctccagtc aatcttgctc tggtcaacgt gaaagaggag aggagtcata ctgagtaggg
    18361 cttggagttg ttctgctgtg atagtcttag tcagagaggc agtttgacta gataggatgg
    18421 taaagtgttc ctggaaatgc tccttgatat cttggttaga atactccttg tttgtcagct
    18481 ggtcctgtac tctttgacgg atttctttga ggtgactttc agttgggata acctttatca
    18541 agataccgtc tttggataaa acgcgacgaa attctccata gttggcaggt gagaagatat
    18601 caagcagaat atccatgtta gcgtctttta taggaagtcg tgccaagtcg ccaacgaacc
    18661 aattgactgc ccagttgggt tcacttttag cagcgatttg gactgaatct ttggagatgt
    18721 caaaggcata gaaagttttt tcagagtgac tttcttgtag tttgcgagaa tagaatcctt
    18781 caccacaacc gatatccaaa attgttgtgg tagtttttga gcttgcaagc aagtcagata
    18841 cagcatctaa gatagcttgg taaaagccgg cttctaggat ttgttgacgg ttttgaaaat
    18901 tttctttgtc atagttagca gattgcttga tttgaggtgc tagattgaca tagccaaatt
    18961 tcgccaagtc aaaagaatga cggttgcagc acttgaaatt agtctctaac agagtcagat
    19021 tttcttgaca gataggacag gcaaaggcag tcgcagaagc aaaacgctga agtttgggct
    19081 tgagatttgt attcatagtt taagtgtatc aaaagaggca aatgaaagca actttaggaa
    19141 taagtagatg ggagattcag tagaaataaa actaattctc caatttatag aatgaaattg
    19201 cttttatatc taaattgcta tataataatg ataaggagga aataagtatg ttaaaagaag
    19261 tattaaccgt tgcaaaagtt gcgaaaaaat cttcattatt tttgggtggt gtcgcatttg
    19321 gtacccttgg tttgaaaatc ttagcaagta aggaagctaa aaaaggttat tctaaagctt
    19381 tggctaaggc ttacaagttg aaagacgagc tagatgcatc tgtttctgtt gtgaagcaac
    19441 atggagacga tgtcttgcaa gatgccaaat atttgtacga gcaagagaaa aaagaagagc
    19501 aattagatag ccttataggt gaataatatg tcttttaaag tgctacatag aggataccaa
    19561 catatccgac tatcatcttc tttttcactt accttggata ttcaagacta tcttcgttcc
    19621 ttggcgagag atgaaaaggg gattgagtct atccagtttt acatggatca acagcacttt
    19681 actctacgca taaaagaagg cttttctgta ttagataatg cagaagcctt tttaaaaaga
    19741 attgataaag ggaaagtttc tgagttgatg actcttccca ttcgtagaga agagagtgct
    19801 tattctattg tttcaggtgc agcggttaag cgtgtacttt ttcgtagttt tgtgccgtat
    19861 cctattcgct atatatggac ttgttatcag gctttgggtt atattagaga agcctatcaa
    19921 acactagcgc gtaaggaact aacgatggaa gtcttggact gttcggctat tttattgtcc
    19981 ttgtttatga accaatccaa gacagctagc aatatcatgt ttatgcttga tttggggaat
    20041 catttagatc agtggtcctt gaaaaaaact acaacagatt tagaacagag tcttcttgca
    20101 aaagagagcg atgtattcct agtacagggc gatacggttg ttagtataaa gagttccgat
    20161 gttcaaatag gagatgtctt gatcttatct caaggaaatg aaattctgtt tgatgggcaa
    20221 gtagtttcag gtttaggtat ggtcaacgaa agttccttga caggagagag ttttccagtt
    20281 gaaaaaagag agtctgattt ggtttgtgca aatacagtat tagaaactgg agagttacgc
    20341 attcgtgtaa cagataatca gatgaacagc cgtattttac agctgattga gttgatgaag
    20401 aaatctgaag aaaacaagaa aacgaaacaa cgctatttca tcaagatggc ggataaagtc
    20461 gtcaaatata atttcttggg ggctgggctg acttacctat tgacaggttc tttttctaag
    20521 gctatttctt tcctattggt cgatttctcc tgcgctttga aaatctctac tcctgtagct
    20581 tatttgacag ttatcaagga agggttgaac cgtgaaatgg tgattaagga tggagatgtt
    20641 ctggagaaat atctggaagt tgatactttc ttgtttgata agacaggaac aatcacaact
    20701 agttatccta tagttgaaaa ggtgttacct tttggagact atagtgagga agatattctc
    20761 agaatcagtg cctgtcttga ggaacacatt tatcatccta ttgctaatgc catcgtcaag
    20821 caagctgaga tagagggaat tgaacatgag gaaatgcatg ggaaactcca atatatcgca
    20881 agcaagggga tcaaatctca tatagatggc caaccagttc ttattgggaa ttatgtcttg
    20941 atgcaggatg agcagattca tatcagttca gaacaaaatg ctttaattga agagtacaag
    21001 agtcactaca atctcttatt cttggcttat caaaatgaat tgattggaat gttctgcatt
    21061 catactcctt tgagaaaaga agcaaaaaca gccttggata aacttaaggc acaggggaaa
    21121 aaattaattc tggcaacagg agacaccttg attaggacag aggaattagt caaagatttg
    21181 ccctttgatc aggtctatac agacttgaaa cctgatggga aatttgagtt agtagagaaa
    21241 ctgcagaaag caggtcacac tattttgatg gttggagatg gattgaatga ctcagcggct
    21301 ttaactctat cagatatcgg tgtggtgatg aatgagagtg cagatatttc taagcagatg
    21361 agtgatatct tattgttaga taatcgtttg gatttcttcc aagagttgga ttcgttatca
    21421 tcatctttgc aaacactcat caagaaaaat attcaagata ccgttgtcgt aaatagtagt
    21481 ttgattggct ttggcttatt taattggttc agtccttcaa atctctctat cttacataat
    21541 ctaacaacct tgcgcattgt cttgcgtagc ttgtctatta aaaatagata gatgagagtt
    21601 attaacagca aaaaggagtt acctttctcg aggttaactc ctttgtttat agataaatgt
    21661 tgaatagttt agtaagtcaa tacaaagtat tttttcttcc cacgacggat aacagtcagt
    21721 tcattttcta acttatcagc gtcactcaag acatagtcaa ggtcttggat gcggtcgccg
    21781 tttacgtaga tggctccgtt ttggacgtct tcacgggctt ggcgttttga gttaactata
    21841 ccagatgaga cgagcagttc cacgatattg ttatgttcgt ctgcctgtac ttggtagttt
    21901 ggcacaccac gaagtccttg tttgagctct ttgacagaaa ggtttttgat gtttcctgca
    21961 aagagttgct cagtgatgtt aagtgcttct ttgtaggctt cttctccgtg aacaagtgta
    22021 acaacttcac gagccaagac tttttgagcc aagcgttcgt gtggcgctgc ttcaaattgt
    22081 ttacgaatat cttcaatctc atcaagtgac aagaaagtaa agattttcaa gaagcgaaca
    22141 gcgtcagcgt ccatcacgtt catccagaat tggtacattt cgtatggaga agtcttttcg
    22201 ggattgagcc atacggcatt tccttctgat ttaccaaatt tcttaccagt tgcatctgtg
    22261 attagtggaa cagtgataac gtgaccagtc ttgtccgcct tacgacgaag caattcggta
    22321 ccagctgtca tatttcccca ctggtcagaa ccaccgattt gaagagtgac attatggtct
    22381 tggttaagga cgaagaagtc gtacccttgc atgatttggt aagcgaactc agtgtaagaa
    22441 attcctgttt cgatccgttt tttaacagat tccttactca tcatgtagtt gaccgtgaag
    22501 tattttccaa tatcacggag gaagtcaatg aagctgatgc tgccaaacca gtcgtagttg
    22561 ttgaccatga cagccttgtt ttcgccattt tcaaagtcaa gaaaacgaga aagttgtcct
    22621 tggatagact tgacccagcc atctactgtg tcttttgttt ggagactacg ttcagcatct
    22681 ttgaaggacg gatctccgat gagacctgta gcaccgccaa cgagcgcata aggtttgtga
    22741 cctgctagtt gcaagcgacg acttgtcaag attgcgacaa ggtggcctag gtgaaggctg
    22801 tcagcagttg gatcgtagcc agtataataa gaaacttgac cttcttctag ggctttacgc
    22861 aaagcttctt catcagtcgt ttgaaatatc aaaccacgct cttttagctc atcaaaaatg
    22921 tgcatgtgtc ttttctcctt tttaaaatta ttgtttctac ctattgtatc acaaacttgg
    22981 gcaatagcct agcagaatag ggaagaaagt ggctatttta ttgaaaattt cccttttgtg
    23041 gtataataga tagagtgagg aaatccatgc aaaatcaatt aaatgaatta aaacgaaaaa
    23101 tgctggaatt tttccagcaa aaacaaaaaa ataaaaaatc agctagacct ggcaagaaag
    23161 gttcaagtac caaaaaatct aaaaccttag ataagtcagc cattttccca gctattttac
    23221 tgagtataaa agccttattt aacttactct ttgtactcgg ttttctagga ggaatgttgg
    23281 gagttgggat tgctttgggg tacggagtgg ccttatttga caaggttcgg gtgcctcaga
    23341 cagaagaatt ggtgaatcag gtcaaggaca tctcttctat ttcagagatt acctattcgg
    23401 acgggacggt gattgcttcc atagagagtg atttgttgcg cacttctatc tcatctgagc
    23461 aaatttcgga aaatctgaag aaggctatca ttgcgacaga agatgaacac tttaaagaac
    23521 ataagggtgt agtacccaag gcggtgattc gtgcgacctt ggggaaattt gtaggtttgg
    23581 gttcctctag tgggggttca accttgaccc agcaactaat taaacagcag gtggttgggg
    23641 atgcgccgac cttggctcgt aaggcggcag agattgtgga tgctcttgcc ttggaacgcg
    23701 ccatgaataa agatgagatt ttaacgacct atctcaatgt gtctcccttt ggccgaaata
    23761 ataagggaca gaatattgca ggggctcggc aagcagctga gggaattttc ggtgtagatg
    23821 ccagtcagtt gactgttcct caagcagcat ttttagcagg acttccacag agtcccatta
    23881 cttactctcc ttatgaaaat actggggagt tgaagagtga tgaagaccta gaaattggct
    23941 taagacgggc taaggcagtt ctttacagta tgtatcgtac aggtgcatta agcaaagacg
    24001 agtattctca gtacaaggat tatgacctta aacaggactt tttaccatcg ggcacggtta
    24061 caggaatttc acgagactat ttatacttta caactttggc agaagctcaa gaacgtatgt
    24121 atgactatct agctcagaga gacaatgtct ccgctaagga gttgaaaaat gaggcaactc
    24181 agaagtttta tcgagatttg gcagccaagg aaattgaaaa tggtggttat aagattacta
    24241 ctaccataga tcagaaaatt cattctgcca tgcaaagtgc ggttgctgat tatggctatc
    24301 ttttagacga tggaacaggt cgtgtagaag tagggaatgt cttgatggat aaccaaacag
    24361 gtgctattct aggctttgta ggtggtcgta attatcaaga aaatcaaaat aatcatgcct
    24421 ttgataccaa acgttcgcca gcttctacta ccaagccctt gctggcctac ggtattgcta
    24481 ttgaccaggg cttgatggga agtgaaacga ttctatctaa ctatccaaca aactttgcta
    24541 atggcaatcc gattatgtat gctaatagca agggaacagg aatgatgacc ttgggagaag
    24601 ctctgaacta ttcatggaat atccctgctt actggaccta tcgtatgctc cgtgaaaagg
    24661 gtgttgatgt caagggttat atggaaaaga tgggttacga gattcctgag tacggtattg
    24721 agagcttgcc aatgggtggt ggtattgaag tcacagttgc ccagcatacc aatggctatc
    24781 agaccttagc taataatgga gtttatcatc agaagcatgt gatttcaaag attgaagcag
    24841 cagatggtag agtggtgtat gagtatcagg ataaaccggt tcaagtctat tcaaaagcta
    24901 ctgcgacgat tatgcaggga ttgctacgag aagttctatc ctctcgtgtg acaacaacct
    24961 tcaagtctaa cctgacttct ttaaatccta ctctggctaa tgcagattgg attgggaaga
    25021 ctggtacaac caaccaagac gaaaatatgt ggctcatgct ttcgacacct agattaaccc
    25081 taggtggctg gattgggcat gatgataatc attcattgtc acgtagagca ggttattcta
    25141 ataactctaa ttacatggct catctggtaa atgcgattca gcaagcttcc ccaagcattt
    25201 gggggaacga gcgctttgct ttagatccta gtgtagtgaa atcggaagtc ttgaaatcaa
    25261 caggtcaaaa accagggaag gtttctgttg aaggaaaaga agtagaggtc acaggttcga
    25321 ctgttaccag ctattgggct aataagtcag gagcgccagc gacaagttat cgctttgcta
    25381 ttggcggaag tgatgcggat tatcagaatg cttggtctag tattgtgggg agtctaccaa
    25441 ctccatccag ctccagcagt tcaagtagta gttctagcga tagcagtaac tcaagtacta
    25501 cacgaccttc ttcttcaagg gcgagacgat a
//
LOCUS       DBERDK010000023        24633 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.023, whole genome shotgun sequence.
ACCESSION   DBERDK010000023 DBERDK010000000
VERSION     DBERDK010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 24633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24633
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.023"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(4..300)
                     /locus_tag="ACL73L_000971"
     CDS             complement(4..300)
                     /locus_tag="ACL73L_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0771969.1"
                     /translation="MVKINKICSIQGSSVENEDIVGSQNQYFWIIGGATDLYNSKEEI
                     GYSVSEVVHILSESLSVNCKESKTLKQIFETALLEVKDEIGLNSYELTEYNKMK"
     gene            complement(284..2443)
                     /gene="pfbA"
                     /locus_tag="ACL73L_000972"
     CDS             complement(284..2443)
                     /gene="pfbA"
                     /locus_tag="ACL73L_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multi-ligand-binding adhesin PfbA"
                     /protein_id="HGS0771970.1"
                     /translation="MLKIVKKLEVLMKYFVPNEVFSIRKLKVGTCSVLLAISILGSQG
                     ILSDEVVTSSSPMATKESSNAITNDLDNSPTVNQNRSAEMIASNSTTNGLDNSLSVNS
                     ISSNGTIRSNSQLDNRTVESTVTSTNENKSYKEDVISDRIIKKEFEDTALSVKDYGAV
                     GDGIHDDRQAIQDAIDAAAQGLGGGNVYFPEGTYLVKEIVFLKSHTHLELNEKATILN
                     GINIKNHPSIVFMTGLFTDDGAQVEWGPTEDISYSGGTIDMNGALNEEGTKAKNLPLI
                     NSSGAFAIGNSNNVTIKNVTFKDSYQGHAIQIAGSKNVLVDNSRFLGQALPKTMKDGQ
                     IISKESIQIEPLTRKGFPYALNDDGKKSENVTIQNSYFGKSDKSGELVTAIGTHYQTL
                     STQNPSNIKILNNHFDNMMYAGVRFTGFTDVLIKGNRFDKKVKGESVHYRESGAALVN
                     AYSYKNTKDLLDLNKQVVIAENIFNIADPKTKAIRVAKDSAEYLGKVSDITVTKNVIN
                     NNSKETEQPNIELLRVSDNLVVSENSIFGGKEGIVIEDSKGKITVLNNQFYNLSGKYI
                     SFIKSNANGKEPVIRDSDGNFNIVTENGLYKIVTNNLSDKNEKEKNKEEKQSNSNNVI
                     DSNQKNGEFNSSKDNRQMNDKIDNKQDNKTEEVNYKIVGDGRETENHINKSKEIVDVK
                     QKLPKTGSNKIMELFLTVTGIGLLLTLKGLKYYGKDK"
     gene            complement(3574..4800)
                     /locus_tag="ACL73L_000973"
     CDS             complement(3574..4800)
                     /locus_tag="ACL73L_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HGS0771971.1"
                     /translation="MPNYNIPFSPPDITEAEIAEVADTLRSGWITTGPKTKELERRLS
                     LYTQTPKTVCLNSATAALELILRVLEVGPGDEVIVPAMTYTASCSVITHVGATPVMVD
                     IQADTFEMDYDLLEQAITEKTKVIIPVELAGIVCDYDRLFQVVEKKRDFFTASSKWQK
                     AFNRIVIVSDSAHALGSTYKGQPSGSIADFTSFSFHAVKNFTTAEGGSATWKANPVID
                     DEEMYKEFQILSLHGQTKDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYP
                     SLLQRRKDIVDRYDSGFAGSRIHPLAHKTETVESSRHLYITRVEGASLEERNLIIQEL
                     AKAGIASNVHYKPLPLLTAYKNLGFDMTNYPKAYAFFENEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTLSKK"
     gene            complement(4843..5535)
                     /locus_tag="ACL73L_000974"
     CDS             complement(4843..5535)
                     /locus_tag="ACL73L_000974"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HGS0771972.1"
                     /translation="MLKWEDLPVEMKSSEVESYYQLVSKRKGSLIFKRCLDWVLALVL
                     LVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRRFKIWKFRTMVTDADKKGSLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSPEMMATLLL
                     QAGITSPASINYKDEDTIISQMTEKGLSVDQAYVEHVLPEKMRYNLAYLREFSFFGDI
                     KIMFQTVFEVLK"
     gene            complement(5708..7459)
                     /locus_tag="ACL73L_000975"
     CDS             complement(5708..7459)
                     /locus_tag="ACL73L_000975"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0771973.1"
                     /translation="MQNKQEQWTVLKRLMSYLKPYGLLTFLALSFLLATTVIKSVIPL
                     VASHFIDQYLSNLNQLAVTVLLVYYGLYILQTVVQYVGNLLFARVSYSIVRDIRRDAF
                     ANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTALVLLFLPLIFLLVNLYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQAEFDEINQEHLAYANRSVALDALFLRPAMSLLKLLGYAVLMTYFGYRGLSIG
                     ITAGTMYAFIQYINRLFDPLIEVTQNFSTLQTSMVSAGRVFALIDERTYEPLQENGQA
                     KVKEGNIRFEHVCFSYDGKHPILDDISFSVNKGETIAFVGHTGSGKSSIINVLMRFYE
                     FQSGRVLLDDVDIRDFSQEELRKNIGLVLQEPFLYHGTIKSNIAMYQEISDEQVQAAA
                     AFVDADSFIQELPQGYDSPVSERGSSFSTGQRQLLAFARTVASQPKILILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEELLTLGG
                     TYHKMYSLQAGAMAYTL"
     gene            complement(7449..9194)
                     /locus_tag="ACL73L_000976"
     CDS             complement(7449..9194)
                     /locus_tag="ACL73L_000976"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0771974.1"
                     /translation="MSIIQKLWWFFKLEKRRYLVGIVALVLVSVLNLIPPMVMGRVID
                     AITSGQLTQQDLLLSLFYLLLAAFGMYYLRYVWRMYILGTSYCLGQIMRSRLFKHFTK
                     MSSAFYQTYRTGDLMAHATNDINALTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYATSRLGRKTHKAFGESQTAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLLFVGSSYVLTLLVGSLMVQEGQITVGN
                     LVTFISYLDMLVWPLMAIGFLFNTTQRGKVSYQRIENLLSQESPVQDPEFPLDGIENG
                     RLEYAIDSFAFENEETLTDIHFSLAKGQTLGLVGQTGSGKTSLIKLLLREYDVDKGAI
                     YLNGHDIRDYRLTDLRSLMGYVPQDQFLFATSILDNIRFGNPNLPLSAVEEATKLARV
                     YQDIVDMPQGFDTLIGEKGVSLSGGQKQRLAMSRAMILDPDILILDDSLSAVDAKTEY
                     AIIDNLKETRKDKTTIITAHRLSAVVHADLILVLQNGQIIERGRHEDLLALDGWYAQT
                     YQSQQLEMKGEEDAE"
     gene            complement(9400..9696)
                     /locus_tag="ACL73L_000977"
     CDS             complement(9400..9696)
                     /locus_tag="ACL73L_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HGS0771975.1"
                     /translation="MSASDVALKLRSQGIFQMKQVKRAVQEQNGQLIVVQMGDENPKY
                     PVVTDGVIQVDVLESIGRSEEWLLDNLSKQGHDNVANIFIAEYDKGAVTVVTYE"
     gene            complement(9849..10304)
                     /locus_tag="ACL73L_000978"
                     /pseudo
     CDS             complement(9849..10304)
                     /locus_tag="ACL73L_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 family protein"
     gene            10438..11265
                     /locus_tag="ACL73L_000979"
     CDS             10438..11265
                     /locus_tag="ACL73L_000979"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HGS0771976.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQE
                     TKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTVTFPEI
                     GAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDVKYAEYLAELDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL"
     gene            11277..11420
                     /locus_tag="ACL73L_000980"
     CDS             11277..11420
                     /locus_tag="ACL73L_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HGS0771977.1"
                     /translation="MDYNAVIPEFLVSNIEQSRSFYCGLLGFRIEYQRPEENFLFLLK
                     SVN"
     gene            11420..11659
                     /locus_tag="ACL73L_000981"
     CDS             11420..11659
                     /locus_tag="ACL73L_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HGS0771978.1"
                     /translation="MLEEGTKDQLAELTYPFGRGVNLSFGIKDVPKLYQKVMEANYPI
                     YRPLTKRKFRVSDPYIYPHKFAVLDPDGYFLRFSE"
     regulatory      12042..12137
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            12537..13118
                     /locus_tag="ACL73L_000982"
     CDS             12537..13118
                     /locus_tag="ACL73L_000982"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HGS0771979.1"
                     /translation="MKLLEERILKDGHILGDNILKVDSFLTHQVDFSLMREIGKVFAE
                     KFAATGITKVVTIEASGIAPAVFTAEALNVPMIFAKKAKNITMNEGILTAQVYSFTKQ
                     VTSTVSIAGKFLSPEDKVLIIDDFLANGQAAKGLIQIIEQAGATVQAIGIVIEKSFQD
                     GRDLLEKAGYPVLSLARLDRFENGQVVFKEADL"
     gene            13118..14380
                     /locus_tag="ACL73L_000983"
     CDS             13118..14380
                     /locus_tag="ACL73L_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HGS0771980.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSAEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLGVAFQSVAPLIMIGQSHGSGAMFGAL
                     IASGIYVVLVSGIFSKVANLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LAAITVLIILLINIFTKGFIKSISILIGLVVGTAIAATMGLVDFSPVAVAPLVHVPTP
                     LYFGMPTFEISSIVMMCIIATVSMVESTGVYLALSDITKDPIDSTRLRNGYRAEGLAV
                     LLGGIFNTFPYTGFSQNVGLVKLSGIKKRLPIYYAAGFLVLLGLLPKFGALAQIIPSS
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIVVASLLAIVLNAVLNHKKK"
     gene            complement(14431..14892)
                     /locus_tag="ACL73L_000984"
     CDS             complement(14431..14892)
                     /locus_tag="ACL73L_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DpnD/PcfM family protein"
                     /protein_id="HGS0771981.1"
                     /translation="MKTKQLVASEEVYDFLKVIWPDYETESRYDNLSLIVCTLSDPDC
                     VRWLSENMKFGDEKQLALMKEKYGWEVGDKLPEWLHSSYHRLLLIGELLESNLKLKKY
                     TVEITETLSRLVSIEAENPDEAERLVREKYKSCEIVLDADDFQDYDTSIYE"
     gene            complement(14889..15653)
                     /locus_tag="ACL73L_000985"
     CDS             complement(14889..15653)
                     /locus_tag="ACL73L_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-2 restriction enzyme DpnI"
                     /protein_id="HGS0771982.1"
                     /translation="MELHFNLELVETYKSNSQKARILTEDWVYRQSYCPNCGNNPLNH
                     FENNRPVADFYCNHCSEEFELKSKKGNFSSTINDGAYATMMKRVQADNNPNFFFLTYT
                     KNFEVNNFLVLPKQFVTPKSIIQRKPLAPTARRAGWIGCNIDLSQVPSKGRIFLVQDG
                     QVRDPEKVTKEFKQGLFLRKSSLSSRGWTIEILNCIDKIEGSEFTLEDMYRFESDLKN
                     IFVKNNHIKEKIRQQLQILRDKEIIEFKGRGKYRKL"
     gene            complement(15834..16238)
                     /locus_tag="ACL73L_000986"
     CDS             complement(15834..16238)
                     /locus_tag="ACL73L_000986"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HGS0771983.1"
                     /translation="MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMD
                     VDITNQEGRNVCKATFTMFVTGQRSEERRVSI"
     gene            complement(16308..17789)
                     /locus_tag="ACL73L_000987"
     CDS             complement(16308..17789)
                     /locus_tag="ACL73L_000987"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HGS0771984.1"
                     /translation="MTLVDKFVTHVISESSFEEMDRIYLTNRVLSRVGEGVLEVETNL
                     DKLIDLKDQLVEETVRLETIEDSQTAREILGTELMDLVTPCPSQVNRDFWEAYAYSPE
                     QAIEDFYQLSQKNDYIKLKAIARNIAYRVPSDYGELEITINLSKPEKDPKEIVAAKLV
                     QASNYPQCQLCLENEGYHGRVNHPARSNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVDQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKAFRFAGFEQVKAGIVKWPMSVLRLTSDSKEDLINLADKILQEWRQYSDSSVQ
                     ILAETDGTPHHTITPIARKRDGQFELDLVLRDNQTSAEHPDGIYHPHKDVQHIKKENI
                     GLIEVMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDLTDKEKAL
                     AIVKDSVGAIFVRVLEDAGVYKQTEQGQTAFMRFVEQVGILLD"
     gene            complement(17808..18986)
                     /locus_tag="ACL73L_000988"
     CDS             complement(17808..18986)
                     /locus_tag="ACL73L_000988"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HGS0771985.1"
                     /translation="MTQHLTAETLRKDFLAVFGQEADQTFFSPGRINLIGEHTDYNGG
                     HVFPAAISLGTYGAARKRDDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQEAGHVIDKGFDFYVYGNIPNGSGLSSSASLEILTGVVAEHLFDLKLERLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVALDIQTLGELDEWAVDQYSYLIKDENRLKRAR
                     HAVLENQRTLKAQAALQAGDLETFGRLMNASHVSLEHDYEVTGLELDTLVHTAWAQEG
                     VLGARMTGAGFGGCAIALVQKDTVEAFKEAVGKHYEEVVGYAPSFYIAEVAGGTRVLD
                     "
     gene            19150..20157
                     /gene="galR"
                     /locus_tag="ACL73L_000989"
     CDS             19150..20157
                     /gene="galR"
                     /locus_tag="ACL73L_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HGS0771986.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAI
                     KQVVDYFLSQGMDRIGILTGLEETTDQEEIIEDKRLENFRNYSQAKGIYHDELIFQGN
                     FTAQSGYDLMKEAIQNLGDQLPPAFFAASDSLAIGALRALQEAGISLPDRVSLISFND
                     TSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHGRKIPSLTMLGTRLTLRESTLNQ
                     E"
     gene            complement(20203..21240)
                     /locus_tag="ACL73L_000990"
     CDS             complement(20203..21240)
                     /locus_tag="ACL73L_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HGS0771987.1"
                     /translation="MKSAVYTKAGQVGLASIERPQIIEADDVIIRVVRACVCGSDLWR
                     YRNPETKAGHKNSGHEAIGIVEEAGEAITTVKPGDFVIVPFTHGCGECDACLAGFDGS
                     CDNHIGNNLGGDFQAEYIRFHYANWALVKIPGQPSDYTEGMLKSLLTLADVMPTGYHA
                     ARVANVQKGDKVVVIGDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATAVV
                     AERGQEGITKVREILGGGADAALECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRAL
                     GSTFMQNISVAGGAASATTYDKQFLLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDE
                     RKTIKSMIVIE"
     gene            complement(21241..21594)
                     /gene="nmlR"
                     /locus_tag="ACL73L_000991"
     CDS             complement(21241..21594)
                     /gene="nmlR"
                     /locus_tag="ACL73L_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stress response transcriptional regulator NmlR"
                     /protein_id="HGS0771988.1"
                     /translation="MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDI
                     EALEFIKCFRSAGVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTAL
                     NRLNLKIKLYKEGKF"
     gene            complement(21838..22728)
                     /gene="czcD"
                     /locus_tag="ACL73L_000992"
     CDS             complement(21838..22728)
                     /gene="czcD"
                     /locus_tag="ACL73L_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter
                     CzcD"
                     /protein_id="HGS0771989.1"
                     /translation="MKAKYAVWVAFFLNLTYAIVEFIAGGVFGSSAVLADSVHDLGDA
                     IAIGISAFLETISNREEDNQYTLGYKRFSLLGALVTAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINLLASLVVGKGKTKNESILSLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLVISFFILSKALPRFWSTLKIFLDAVPEGLDIKQVKSGLERLDNV
                     ASLNQLNLWTMDALEKNAIVHVCLKEMEHMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
     gene            22861..23397
                     /gene="sczA"
                     /locus_tag="ACL73L_000993"
     CDS             22861..23397
                     /gene="sczA"
                     /locus_tag="ACL73L_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator SczA"
                     /protein_id="HGS0771990.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKDYETTTVQDIIDLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISTEDYLAHLFLHFHKNQDHITSLLFS
                     KNDYFLRQLQRELEHHVYSVLADNLKEAHPNLPTSYLQHLVMSNFIETLTWWLKKGQD
                     FTDQEVVQFYLDLLIPKN"
     gene            complement(23563..24369)
                     /gene="pnuC"
                     /locus_tag="ACL73L_000994"
     CDS             complement(23563..24369)
                     /gene="pnuC"
                     /locus_tag="ACL73L_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HGS0771991.1"
                     /translation="MHTYLQKKIENIKTTLGEMSGGYRRMVAAMADLGFSGTMKAIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELVYEHRIVDWIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQAQFKKEKQEFVARK
                     LDGKGWTKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLLN"
BASE COUNT         7307 a         5498 c         4466 g         7362 t
ORIGIN      
        1 ttattatttc attttattat actctgttaa ttcatatgag tttaaaccga tttcatcttt
       61 aacctcgagt aaagcagttt caaatatttg tttaagagtt tttgattctt tacaattaac
      121 cgacaaactt tctgataaaa tatgtacaac ttctgagact gaataaccta tctcctcttt
      181 agaattatat aaatctgtag ctccaccaat aatccaaaaa tactgatttt gtgaacctac
      241 aatatcctca ttttctacgg aacttccttg tatcgaacaa attttattta tctttaccat
      301 aatacttcaa cccttttagt gtcaaaagta aaccaattcc tgtcactgtt aagaatagtt
      361 ccataatctt gttcgaacca gtctttggta atttttgttt tacatctact atttctttag
      421 atttattaat atgattttca gtttctctgc catctccaac tattttatag tttacttctt
      481 ctgtcttatt atcttgttta ttgtcgatct tgtcattcat ttgtctatta tctttacttg
      541 agttaaactc tccgttcttc tggttactat caattacatt atttgaatta gattgttttt
      601 cctctttgtt tttttctttt tcgtttttat cacttaaatt atttgttaca attttgtaaa
      661 gcccattctc cgttacaata ttgaaattac catcgctatc acgtataaca ggttctttcc
      721 cgtttgcatt agatttgatg aatgatatat acttaccgga taaattataa aattggttat
      781 ttaaaacggt tattttaccc tttgaatcct caataacaat tccttcttta ccaccgaata
      841 tactattctc tgacactact aaattatcac taactcgtaa taattcaata tttggttgtt
      901 ctgtttcctt agaattatta ttaattacat tttttgttac agtaatatct gatacttttc
      961 ctaaatattc tgcactatct tttgcaactc gtatcgcttt tgttttagga tcggcaatat
     1021 taaatatatt ttcggcgata accacctgtt tatttaaatc taataggtct ttagtgtttt
     1081 tatagctata agcatttact aaagctgctc cgctttctcg ataatgtaca ctctctcctt
     1141 taactttctt atcaaagcga tttcctttga ttaatacatc agtgaatcct gtaaaacgta
     1201 cacctgcata catcatgtta tcaaaatgat tatttaaaat tttaatatta gaggggttct
     1261 gtgtcgacaa tgtttgatag tgtgtgccaa ttgctgttac taattcccca gatttatcac
     1321 ttttgccaaa ataggaattt tgaatagtca cattttcaga ttttttccca tcatcattca
     1381 aggcataagg aaaacctttt ctagttaatg gttcaatctg aatgctctcc ttacttatga
     1441 tttgcccatc cttcatcgtt ttgggtaagg cttgcccaag aaaacgagaa ttatcaacta
     1501 atacattttt cgaacctgca atttgaatag catgcccttg ataactatcc ttgaatgtta
     1561 cattttttat agttacgtta tttgaattcc caatagcaaa tgcacctgaa gaatttataa
     1621 gtggtagatt ttttgcttta gttccttctt cattcaaagc accgttcata tcaatcgtac
     1681 caccagaata actaatatct tctgttgggc cccattctac ttgcgcacca tcatccgtaa
     1741 ataaacctgt cataaaaaca atggaagggt gattcttaat atttatacca tttagaattg
     1801 tagctttctc attcaattct aagtgtgtat gactttttaa aaaaacaatt tcttttacta
     1861 aataagttcc ttcaggaaaa tatacatttc ctccacctag cccttgagct gcagcatcta
     1921 ttgcatcttg aattgcttgt cgatcatcat gaatcccatc acctaccgca ccataatctt
     1981 ttacacttaa agcagtatct tcaaattctt ttttgataat tctgtcactt ataacatctt
     2041 ccttataact cttattttca ttagtagatg ttactgtaga ttcaactgtt ctgttgtcta
     2101 attgtgaatt ggaacgaata gtaccattag agctgatact attaacactt aacgaattat
     2161 ctaaaccatt agtggttgaa ttagaggcaa tcatttcagc agaacgattc tgattaacag
     2221 ttggtgaatt atctaaatca ttagtaattg cattagaaga ctcttttgta gccatcggtg
     2281 aagaactagt aacaacttca tccgataaaa taccttggct tcccaaaatt gaaattgcca
     2341 atagtaccga gcaagtcccc acctttaatt tacgaatact gaatacctca ttaggaacaa
     2401 aatatttcat cagaacctcc aattttttta ctatttttaa caattgatta ttacttttct
     2461 taaaatagat agaatgacac cgctgtcatt atttttatca tatattttcc aaattgaatt
     2521 gtcaaggaat aacacaagaa aatattaaaa tttatactct tcggaaatct cttcaaacta
     2581 ggtcagtgcc gccttgccct aagtctaggt tactgacttg gtcagttcta tagtagactg
     2641 aaactataat agtacacctc tacttctaaa acattgttag aaatcgattt aactgtcccg
     2701 atcaatttgt cctattcttg tttcatttta ctatatctac aacctcaaag cagggttttg
     2761 agctatctat ggccaacttc ctagtttgct ctttagcctt ccattcaaaa gtgatgctat
     2821 ctttcttagc ttgaatataa aatatcccat ttactatatt taaatttgtt gatatgttgt
     2881 aaattataaa tgaaagaaca aataccttgt catacaagca ttcgccaggg atatttacct
     2941 gaatatcgct catatggcgg attgaaacta aaacactaca atatatttct taaatagacc
     3001 ttctataaaa gctagattta acctcgataa aaatagatgc agtgcttcta aaatactgga
     3061 atagggcata taggtaatcg tagacaagtg ttttatagat tgtctcgcag gagatctaat
     3121 gatgtatagc aagcatcact aaacatatgg gatatttgcg ttattataat atcaacatgc
     3181 tataaattct atcatgcaaa agaatgacta gaacatgaaa gacaggcaca aaactttgaa
     3241 caatattctg aaaacaaaaa aaagacgcta gtttccacaa tctcactcat ggaaactcac
     3301 gtctttcaaa aaatagggtg aattgaaaac tagaacaaga cactagattt tctaaatgat
     3361 gtctagtaag gattctaaat gctataccaa attgctcaca tcttatttca aaccacggga
     3421 ctcccacaag aaaggaaaat cctataaaaa ttctctctct gaggagaaag aggcctcaac
     3481 tacaggtgta aactattatc aattgaaaac aacagttttt acactcaatt agacttttta
     3541 gggacaggat cactttcgct tgactgtagt ttgtcatttt tttgataaag ttagcacttt
     3601 ttcagaaact gttttgaaag tctcaatgat atagtctact tcttcatcgc ttaatttagt
     3661 atgaagaggg agggtaattt cattctcaaa gaaggcatag gccttaggat agttcgtcat
     3721 atcaaatcca agattcttat aggctgtcaa gagaggaagc ggtttgtagt gaacattact
     3781 tgcaattcct gctttagcca attcttggat gatgaggttg cgttcttcta ggcttgctcc
     3841 ttctacacgg gtgatgtaga ggtggcgtga agattcgaca gtttcagtct tgtgtgccaa
     3901 aggatggatg cgagaacctg caaaaccact atcatagcgg tccacaatgt ccttacggcg
     3961 ttgcaacaaa cttggatagc ggtccaattg taccaaacca agtgaagcca tgatatcggt
     4021 catgttgcac ttataggctg gtgtaacgat atcgtattcc catgacccca gttgcatctt
     4081 ggcaagagca tccttagttt gcccgtgaag ggaaaggatt tggaattcct tgtacatctc
     4141 ttcgtcatca atcactggat tggctttcca agtcgcactt ccaccttctg ccgttgtaaa
     4201 gttcttaaca gcatggaatg agaaggaagt aaaatcagcg atagaaccag aaggttgtcc
     4261 tttataagta gatcccaaag cgtgggcact atcagagaca atgacaatac ggttaaaggc
     4321 cttttgccac ttgcttgaag cggtaaagaa gtcacgtttt ttctccacga cttggaacaa
     4381 acggtcataa tcgcaaacaa tccctgcgag ctctactggg ataatcacct tagttttctc
     4441 agtgatagct tgctcaagca ggtcatagtc catctcaaac gtatctgctt ggatatccac
     4501 catgacaggg gttgctccca cgtgcgtaat gacactacat gaagccgtat aggtcatggc
     4561 tggaacgatg acttcatcac caggtcccac ttccaaaacg cgtaaaatca actccagagc
     4621 ggctgtcgca gagttgagac aaacagtctt aggtgtctgt gtgtaaagag acaagcggcg
     4681 ctccagttct tttgttttag gacctgttgt gatccaacca gaacgcaggg tatccgctac
     4741 ttcagcaatt tctgcttctg tgatatcagg cggtgaaaat ggaatattgt aatttggcat
     4801 tgatttgctc cttttatctg tactcatttt cttatgacta ctttatttta gtacctcaaa
     4861 cacggtttga aacatgattt tgatgtcccc aaagaaacta aactctcgga gataggcgag
     4921 gttatagcgc atcttttcag gaagaacatg ctccacatag gcctgatcaa ctgacagacc
     4981 tttctccgtc atttgactga tgatggtgtc ctcatccttg tagttgatgc tggctggaga
     5041 ggtaattcct gcttgcaaga gcaaggttgc catcatttca gggctatact gctctgtata
     5101 acgtggcact tcaggtcgtg taccgacaaa ggacatctca cctttaagga cattgaccaa
     5161 ctgaggcagt tcgtccaaac ggacacgtcg gatgaaattt ccaaccttgg taatacggct
     5221 atcgttagca gaagtcacca gacttccttt tttatccgca tccgtcacca tggtacggaa
     5281 cttccaaatc ttgaaccgac ggttgtactg ggtcacacgc tcttgcttgt aaatcactgg
     5341 ccccttgcta tccaacttga tccaaatgct caagatgaga aagatgggag aagttagaac
     5401 cagtaagacc aaggccaaaa cccagtccaa gcaacgcttg aaaatcagcg aacccttcct
     5461 tttagagaca agctggtagt aagactcaac ctcgcttgat ttcatttcca caggcaagtc
     5521 ttcccatttc agcatgctgt tctcctttgt ttttattata ctatttgtca acatttttca
     5581 ttataccaca aaatggaaaa ggcggtgaaa gaaatctgta atttgaagga tttccttcta
     5641 gtactcaatg aaaatcaaag tacagctttg agattgcaga taaagctgac atggtttaaa
     5701 gagattttca aagagtatag gccatggccc ctgcctgcaa actatacatc ttgtgatagg
     5761 ttcctcccag agtcaagagt tcctcatggg ttccactctc gataatgcgt cccttatcca
     5821 agacatagat gcagttggcg tcttgaatag tagaaaggcg gtgagcgata gcaatagttg
     5881 ttcggccctg tctcatcttc gccagagaag cttgaaccaa actttctgtt tcagagtcaa
     5941 tattggctgt cgcttcatcc aaaatcagga ttttaggctg gctggcgact gttctagcaa
     6001 aggcaagaag ctggcgctgc ccagtagaga agctcgaacc acgctcggaa acaggggagt
     6061 cgtacccctg aggaagttct tgaataaagg aatctgcatc cacaaaggct gccgcagcct
     6121 gaacctgctc atcactgatt tcttggtaca tggcgatatt ggacttaata gttccatgat
     6181 agaggaaggg ttcctgtaaa accaaaccga tgttttttct cagctcttct tgactgaaat
     6241 ccctgatatc cacatcatcc aagagaactc tccctgactg gaattcataa aagcgcatga
     6301 ggacattgat aatagacgat ttccctgaac ctgtatgacc tacaaaggca atggtttcac
     6361 ccttattaac agagaaagaa atgtcatcca gaatcggatg tttaccgtca tacgagaaac
     6421 acacatgttc aaaacggata ttgccttctt tgactttggc ttgcccattt tcttgaaggg
     6481 gctcataggt cctctcatcg attaaggcaa agacacggcc tgcagaaacc atagacgttt
     6541 gcagtgttga aaagttttgc gtcacctcaa tcaaagggtc aaagaggcgg ttgatgtact
     6601 ggataaaagc atacatggtc ccggccgtta tcccgataga aagaccacga tagccaaaat
     6661 aggtcatcaa gacagcataa cctagaagtt tcaacaaact catggcaggt ctcaaaaaga
     6721 gggcatccaa ggctacagaa cggttggcat aggccaagtg ctcttggttg atttcatcaa
     6781 attctgcctg caggcgcttc tcttgattaa aagcctgaat aatcctgatt ccctcgatat
     6841 tctctgccag cttactattg atatctgaca agagacttct ggttttctcg atgattttca
     6901 ctgacttttt tcgatagaga ttgaccaaaa ggaaaatcaa ggggagaaag agcaagacta
     6961 atgctgtcaa acgaaaatcc agcaccaaca tggtataaag ggttgtcaga aagataaaaa
     7021 ctgctgagat aaagctggat aaaatcccag aaaacatatc actaatcgtc tcggtatcgt
     7081 tggtcaaacg agaaacgata gaacctgctg gcgtcttgtc aaagtaagac atgcccagtt
     7141 tctccatatt ggcaaaggca tcccgacgaa tatccctaac aatactgtaa gacacgcgcg
     7201 caaagagaag attgccgaca tactgaacta cagtttgtag gatgtagaga ccatagtaga
     7261 ccagcaaaac ggtcacggct agttggttaa gattgctgag atactggtcg ataaagtggg
     7321 aagccacaag gggaatgaca cttttaatga ccgtcgtcgc taggagaaaa ctgagtgcca
     7381 aaaaggtaag gagtccataa ggcttgagat aggacatcaa gcgcttcaat acagtccatt
     7441 gttcttgttt attctgcatc ttcttctccc ttcatttcca gctgctgaga ctggtaggtt
     7501 tgggcatacc agccatccaa agctagcaag tcttcgtgcc tgcctcgttc gataatttga
     7561 ccattttgca ggactaaaat caaatctgca tggacaactg cactcaggcg atgggcagtg
     7621 ataatggttg tcttgtcctt tcgcgtctcc ttgaggttgt cgataatcgc atactctgtc
     7681 ttggcatcta cggcggataa ggaatcatcc aaaatcaaga tatcagggtc taaaatcata
     7741 gcccgactca tagccaaccg ttgcttttga ccaccagaaa gactgactcc tttttcacca
     7801 atcagcgtat caaatccttg aggcatgtct acaatatctt ggtaaactcg ggctagctta
     7861 gtagcttcct cgaccgctga aaggggcaag ttaggattgc caaagcggat attgtctagg
     7921 attgaagtcg caaaaagaaa ctggtcctga ggaacatagc ccatgagact gcgaaggtct
     7981 gtcagacgat agtcccgaat atcgtgaccg tttagataaa tggcgccctt atccacatcg
     8041 tattcacgca agaggagctt gattaaggac gttttcccag agcctgtctg cccaaccaag
     8101 cccagtgttt gcccttttgc caaactaaag tgaatatccg tcagtgtttc ctcattttca
     8161 aaagcaaagc tgtcaatggc atactccaaa cgcccatttt caataccatc cagaggaaac
     8221 tccgggtctt gtacaggaga ttcctgagac aaaagatttt caatccgctg gtaagaaacc
     8281 ttccctcgct gagtagtatt aaagaggaag ccgatggcca taagaggcca gaccagcata
     8341 tccaaatagc tgataaaggt gactagattc ccaactgtaa tctgcccttc ctgaaccatc
     8401 aaggagccaa ccaaaagcgt taaaacatag gacgaaccaa caaacaagag aaccataggg
     8461 tcaaagagac tatcatattt catggtttgc aggttctttt ggaaggttaa ttcattgact
     8521 gcctgaaaag acttcaactc gtctgcctga taaccgaaag acttggtcac tttgatacct
     8581 gatacggact cctgtacctt gttattgagt tcagaaaaag cagtttggga ttcgccaaag
     8641 gccttatgag tctttctccc taggcgacta gtcgcatagg ccatgaaagg tagggggaga
     8701 atggcaacaa gagtcatctg ccatgagatg ctaaagagca tggtcaacaa agtcaccaga
     8761 gccgtgatag aggcatccac cgcagacatg acaccgccac ctgctaaacg agtcaaggca
     8821 ttgatatcat tggttgcgtg tgccatcaga tcacccgtcc gataggtttg ataaaaggct
     8881 gacgacattt ttgtgaaatg cttaaacaag cgagaccgca tgatctgtcc caagcaataa
     8941 gaggtcccaa ggatatacat acgccacaca tagcgcaaat agtacatacc aaaggctgca
     9001 agtagcaagt aaaataggct aagaaggagg tcctgctggg ttaattgccc cgatgtgatg
     9061 gcatcaatga cccgccccat aaccatagga ggaatgagat tgaggacgga aaccaagacc
     9121 agggccacaa tcccgactag ataacggcgt ttttctaact tgaaaaacca ccaaagtttt
     9181 tgaataatgg acataaaatc cctttctgat tgcaaatgga agcctgaggc caatactcaa
     9241 tgaaaatcaa agagcaaact aggaaactag ccgcaggctg ctcaaagcac tgctttgagg
     9301 ttgtagatag aactgacgaa gtcagtaacc tacatacggc aaggcgacgt tgacgcggtt
     9361 tgaagagatt ttcgaagagt acaagacccc aggtttttct tattcataag ttacgactgt
     9421 aacagcaccc ttgtcatatt cagcaataaa gatattggct acattgtcat gcccttgttt
     9481 gctgaggtta tcaagcaacc actcttcgct acgaccaatc gattccaaga catctacttg
     9541 aatcacaccg tcagtcacaa ctggatactt aggattttca tctcccattt gcacaacgat
     9601 gagttgccca ttttgctctt gcacagctcg tttgacttgc ttcatctgga aaatcccttg
     9661 gctacgaagt ttgagggcta catccgatgc agacaaacca actgaacgac aggcttatgg
     9721 gtcaatattc ccatttttga taaggagagt tggcttacca tcaatcaaac gtttcataaa
     9781 gtgaacattg ttgttgaact atttgagagt caagaccagt ttgattaaaa tttcgatata
     9841 attaagtgtc atttgttcac atccaccagt tcaattgcgt ctgtcttata caattcgact
     9901 ttctctaaca ggtacttgtc tggctcactt ccgtttaggg cacgataata gcgatctccc
     9961 accttgataa gtgcgccatc tgtgtttgtg gaagtattaa catagacttc tgacttgtct
    10021 actcccaaat ctttggaaac aacctcgatg aaatgaagtg aagtttgaaa tttattgtta
    10081 gaaacttgat tgttttgata agtcgtgatg ctgaccaaaa gcagagctaa ataaggccag
    10141 agctgaaatc atgaccaact cacgaaattc ggttcccttt ttatcatggt atgccttaaa
    10201 agcaaaaaaa ctgtcacagc taatagaaca atcccaaagc caaccatgat accattttgc
    10261 tgaccgattt ggctgagtac atagtcataa gagtagaatt tcatgtattt tcactccctt
    10321 tcataaaatc attcttaatt ataaacgaaa gagagtaatt tttcaaacca tacgttgggg
    10381 aaatagatct ttttttggta taataaaatc tataatctga atgaaaaagg taactttatg
    10441 aaacttatct catggaatat tgattcccta aacgctgccc taactagtga ctcagctcgt
    10501 gccaaattgt cccaagaagt cctacaaacc ttggtcgctg aaaatgctga tatcattgct
    10561 atccaagaaa ccaagctttc tgccaaagga cctacaaaga aacacgtgga aattttagaa
    10621 gaactcttcc caggctacga aaacacgtgg cgctcttccc aagagcctgc ccgtaaaggc
    10681 tatgctggaa ccatgttcct ttataagaaa gaacttacac ccactgtcac tttcccagaa
    10741 atcggtgccc cttctaccat ggacttggaa ggccgcatca tcactctaga atttgatgca
    10801 tttttcgtga cccaagttta cacaccaaat gctggcgatg gtcttaaacg cttggaagaa
    10861 cgccaagtct gggatgtcaa atatgcggag tatttggctg aactagacaa agaaaaacca
    10921 gtccttgcga ccggtgacta caacgtagcc cacaatgaaa tcgaccttgc aaatcctgct
    10981 agcaaccgcc gttcacctgg atttactgac gaggaacgtg ctggatttac caacctattg
    11041 gcaactggat ttactgatac cttccgccat gttcatggcg atgttcctga acgctacact
    11101 tggtgggcac aacgcagcaa aacttctaaa atcaacaata caggctggag aatcgactac
    11161 tggctcacaa gtaaccgtat cgctgacaag gtcactaaat ctgatatgat tgactcaggt
    11221 gctcgccaag accatacacc gattgtcttg gaaattgact tgtaaggatt ttctatatgg
    11281 actacaatgc ggtcattccc gagtttcttg tatcgaatat cgaacagtcc cggtccttct
    11341 actgtggttt actgggtttt aggattgaat accaacgtcc agaagaaaac tttctctttc
    11401 tcttgaagag tgtcaactaa tgttagagga gggaacaaag gatcaactag ctgaactgac
    11461 ctaccccttt ggtcgtggag tcaatctctc ctttggcata aaggatgttc caaaactcta
    11521 tcaaaaagtg atggaggcaa actatcctat ttatcgtccc ttgaccaaaa gaaagtttcg
    11581 tgtcagtgat ccctatatct atcctcataa atttgcagtt ttggatccag atggctattt
    11641 tttaagattt agcgagtaga ataataaaaa ttgggaaaag tctttaaagt tagaaaaagg
    11701 catattgtca ggtgtttaaa aacttgatag tatgcctttt atgatggaaa aatcttctaa
    11761 tactcttcga aaatctcttc aagccacgtc aacgtcgcct taccgtaggt atatgatact
    11821 gactctgtca attctatcca caacctcaaa acagtgtttt gaggttgtgg atagcttcct
    11881 agtttgctct ttgattttca tttagtataa atgttagctt acaatggagt ttttgttcac
    11941 cctctcttct ttttgttcgc tttttaaacc aaaatactaa caaaatagtt gatttctaaa
    12001 gttatttagt gtaaaataaa aacattggct caagcctatt taaaattgaa tatcgtttta
    12061 cttgtttatg tcgtgaattg gcacgacgtt tctacaaggt gccggaacac ctaacaataa
    12121 gtaagtcagc agtgagatat ggccgttttt gccatgtcta tttaggggac ttacttagag
    12181 attaagtagg tcttttttga atatttttaa tatttccctt aattagtgcg gacgggaggt
    12241 gaaattatcc tgtgaataat ttcaggaatc cagggaattt catccctaat ttttgagccc
    12301 ctagctcttt catttctagg cttaggctaa aatagttccc cgaactattt tactctcaaa
    12361 aatcccgtca tagacaataa caagattatt gtctatgata ccactacaac gggaaatatt
    12421 tgataagact cgcttcgctc gtaatagttt tatcaaatat atagtattag caaaagggat
    12481 cactatagaa atatttaaac aacaagtaaa ttaactttta gactttagga ggtcttatga
    12541 aattattaga agagcgcatc ctcaaggatg ggcatatctt gggtgataac atcctcaagg
    12601 tagattcctt tttaacccac caagttgact ttagcttgat gcgagagatt ggtaaggttt
    12661 ttgcggaaaa atttgctgct actggcatta ccaaggtcgt aaccattgaa gcgtcgggta
    12721 ttgccccagc cgtttttaca gctgaagcct taaacgttcc catgattttc gccaaaaaag
    12781 ctaagaacat caccatgaac gaaggcatct taactgctca agtctactcc tttaccaagc
    12841 aggtgaccag cactgtttct atcgctggaa aattcctctc accagaggac aaggttttga
    12901 ttatcgacga tttccttgct aatggccaag ctgctaaagg cttgattcaa atcatcgaac
    12961 aggccggtgc cacagtccaa gctatcggta tcgtgattga gaaatccttc caagatggtc
    13021 gtgatttgct tgaaaaagca ggctaccctg tcctatcact tgctcgcttg gatcgttttg
    13081 aaaatggtca ggtcgtattt aaggaggcag atctctaatg caaactcaag aaaaacactc
    13141 gcaagcagcc gttctgggct tgcagcactt actagccatg tactcaggat ctatcctggt
    13201 tcccatcatg attgcgacag cccttggcta ttcagctgag cagttgacct acctgatttc
    13261 tacagatatc ttcatgtgtg gggtggcaac cttcctccaa ctccaactca acaaatactt
    13321 tgggattgga ctcccagtcg ttcttggagt tgcattccag tcggtcgctc ccttgattat
    13381 gattgggcaa agccatggta gtggcgctat gtttggtgcc cttatcgcat ctgggattta
    13441 cgtggttctt gtttcaggca tcttctcaaa agtagccaat ctcttcccat ctatcgtaac
    13501 aggatctgtt attaccacga ttggtttaac cttgatccct gtcgctattg gaaatatggg
    13561 aaataacgtt ccagagccaa ctggtcaaag tctcttgctt gcagctatta ctgttctgat
    13621 tatcctcttg atcaacatct ttaccaaagg atttatcaag tctatctcta ttttgattgg
    13681 tctggttgtt ggaactgcca ttgctgctac tatgggcttg gtggacttct ctcctgttgc
    13741 ggtagctcca cttgtccatg tcccaactcc actctacttt gggatgccaa cctttgaaat
    13801 ctcatctatt gtcatgatgt gtatcatcgc aacggtgtct atggttgagt caactggtgt
    13861 ttatctagcc ttgtctgata tcacaaaaga tccaatcgac agcacgcgcc ttcgcaacgg
    13921 ttaccgcgca gaaggtttgg cggtacttct cggaggaatc tttaacacct tcccttatac
    13981 cggattttca caaaacgttg gtttggttaa attgtcaggc atcaaaaaac gcctgccaat
    14041 ctactacgca gctggtttcc tggttctcct tggactgctt cctaagtttg gtgcccttgc
    14101 ccaaatcatt ccaagctccg tcctcggcgg tgccatgctg gtgatgtttg gttttgtatc
    14161 tattcaaggg atgcaaatcc tcgcccgagt tgactttgct aacaatgaac acaacttcct
    14221 tatcgcagct gtttcaatcg ctgcaggtgt cggtctcaac aacagtaatc tctttgtcag
    14281 catgccgaca gccttccaaa tgttcttctc aaacggaatc gtcgtagcca gcctactcgc
    14341 tattgtcctc aatgccgtat taaatcataa aaagaaataa gaaaaagagg tgcgagcctc
    14401 ttttttgtgt gacaaaataa aaacatctac ctattcatat atgctagtgt catagtcctg
    14461 aaaatcatct gcatcaagaa caatttcaca actcttatac ttttctctta caagtcgttc
    14521 ggcttcatct ggattttcag cctctatact tactaaacgt gataaagttt ctgtaatttc
    14581 tactgtatac tttttcagtt ttagattgct ttccaataat tcacctatta acaataatct
    14641 atgataggag ctatgtagcc actctggcaa tttatctcct acttcccacc catatttttc
    14701 cttcatcaaa gctagttgtt tttcgtcacc aaatttcata ttttcagata accatctcac
    14761 acaatcggga tctgataagg tacagacgat taaacttagg ttatcgtaac ggctttcagt
    14821 ttcataatca ggccagatga cttttaagaa atcatacacc tcttctgatg caacaagttg
    14881 tttcgttttc ataatttccg atactttcct ctacctttaa attctattat ttctttgtct
    14941 cttaatattt gaagctgttg cctaatcttt tctttgatat gattgttctt aacaaagata
    15001 ttttttaggt cactttcaaa acgatacata tcttcaaggg taaattctga accctctatc
    15061 ttatctatac aatttagaat ttctattgtc caacctcttg atgacagaga gctcttcctt
    15121 aaaaataaac cttgcttaaa ttcttttgta actttttctg gatctctaac ttgtccatct
    15181 tgcacaagaa atatccttcc tttagaaggt acttgtgata aatcaatgtt acaaccaatc
    15241 caacctgctc gtctagcagt tggtgcaagt ggttttcttt gaataatcga tttcggtgta
    15301 acaaattgct tcggaaggac aagaaagtta tttacctcaa aattttttgt gtaagttaaa
    15361 aaaaagaaat taggattatt atctgcctgc acacgcttca tcatcgttgc ataagcacca
    15421 tcattgattg ttgatgaaaa atttcctttt ttgctcttta gttcaaactc ctcactacaa
    15481 tgattacagt aaaaatctgc tacaggccga ttattttcaa aatgatttaa gggattgttc
    15541 ccacaattag ggcaataaga ctgacgataa acccaatcct ccgttaagat acgtgccttt
    15601 tggctattac ttttataggt ttctactaat tctaaattaa agtgtaattc catttaaaaa
    15661 ttcctcacta atatgagaac tcactcgata taagcaatta aaatctacta taactagtat
    15721 accacatata tgtacctatt acatttgtat ttcatcactc tcttttatat ttccttctaa
    15781 catatctcat aaattaaaaa tgagtgagat aaatttatcc cactcacctt tctttatata
    15841 cttacccgtc tttcctcaga cctttggcct gtgacaaaca tggtaaaggt tgccttacag
    15901 acatttctgc cctcttgatt ggtgatatcc acatccatca cacaggttgt acgaccttga
    15961 tggacacatt ctcctttaat ggtcaataca tcgtcgagtt ttcctgcctt aaggtagttg
    16021 atagaggatt ggagtgtcac cccatcaagt cccagcgaga taaccaccaa accactgatt
    16081 tggtcacata gcgtgaatag atagccacca tgggcattgc catagtagtt gagcgatgag
    16141 ttcactactt tggtcgtcac cacaacgtga ccatctctca ttttttcaat ttcgtaattt
    16201 tcaaaggcag atatagcgtc aaaatgaaag tctttcatcg tttcctcctg atatttcaaa
    16261 cgacactatg atactacttt ttataggact gtgcaaggag aaagctccta gtctagtaaa
    16321 attccgacct gttccacaaa gcgcataaag gctgtctgcc cttgttctgt ctgcttgtag
    16381 actcctgcat cctcaagtac acgcacaaag atagcaccca cagagtcctt gacgattgca
    16441 agggcttttt ctttatccgt taggtctgga tgttgggatt tgagttggtc tgcccactcc
    16501 tgatgataat cggcaactgt aacagcttct cctacaagat agctagcgac ttgctctact
    16561 tcttctttca gacgtggtgg caagattgcc aagcccatga cctcaatcaa gccgatattt
    16621 tccttcttga tatgttggac atccttgtgg ggatgataga tgccatcagg atgctctgct
    16681 gaagtctgat tgtctcgcaa gaccaagtcc aactcaaact gtccatcacg cttacgcgct
    16741 attggtgtga tggtgtgatg cggtgtcccg tctgtctctg ctaaaatctg cacactagaa
    16801 tctgaatact gacgccattc ctgcaaaatc ttatcagcca aattgatcaa atcctctttg
    16861 gaatccgaag tcaaacgtag gacagacatg ggccacttga caattccagc cttgacctgc
    16921 tcaaaaccag caaatcggaa ggccttttgc aagggagcca attccatagg aaatacgtga
    16981 cggcctccct gataatgatc atgagttaga atagagcccc ccacaatcgg caggtcggca
    17041 ttagatccag caaaatatcc tggaaactgg tctacgatag ccaacagacg ttcaaaactc
    17101 tgacgactaa tggccatggg acgatgctgg ccatctaaaa agatacaatg ctcattaaag
    17161 taagcatagg gcgaatactg gaaaccccat tcctgaccaa ccatttcaaa acggataata
    17221 cggtgattgc tacgagctgg gtggttaact cgaccatggt agccctcatt ctctagacaa
    17281 agctgacact gaggataatt actagcttgc accaacttgg ctgccacaat ctctttggga
    17341 tctttttcag gcttagagag attgatggta atttcaagtt ctccgtagtc agatggaaca
    17401 cgataagcga tatttctagc aatggccttg agtttgatgt agtcattttt ctgactgagt
    17461 tgataaaaat cctctatcgc ttgctcagga gagtaggcat aggcttccca gaagtcacga
    17521 ttgacctgac tcggacaagg agtcaccaag tccatcaatt cagtaccaag gatttcacgc
    17581 gcagtctgac tatcctcaat cgtctctaat cgaacggttt cctcaactag ctggtccttg
    17641 aggtcaatca atttatccag attggtctca acttccaaaa caccttctcc cactcgtgac
    17701 aaaacacggt tggtcaggta gattcgatcc atttcctcaa atgaactttc agaaatgaca
    17761 tgtgttacaa atttatctac taaggtcact atagagcctc cttttgacta gtcaaggacg
    17821 cgagtgccac ctgcaacttc agcgatatag aagcttggag cgtatccaac tacttcctcg
    17881 tagtgtttgc ctacagcttc cttaaaggcc tcaacagtat ctttttgaac caaggcaatg
    17941 gcacagccac caaaaccagc ccctgtcata cgagcaccga gaactccttc ttgtgcccaa
    18001 gctgtgtgaa caagggtatc taattccaaa ccagttactt cataatcatg ctccagagaa
    18061 acgtgtgacg cattcatcaa acgaccaaat gtttccaaat cacctgcttg aagggctgct
    18121 tgagctttaa gggtacgttg gttttcaagc acagcatggc gagcacgttt caaacgattt
    18181 tcatctttaa tcagatagct atattggtca acggcccact cgtccaattc acccagggtc
    18241 tgaatatcca aggcaacttg caattcttcc actgcttttt cacactcagc acgacgttca
    18301 ttgtatttag agtccgccaa ttcacggcgt ttgttggtgt tcatgataac aacgacattg
    18361 tccttcaaat caagtggcac caaatcatac tctaaggtgt tggtatcaag gtaaatagca
    18421 cgttgatcag cccccatacc aatagcaaac tggtccatga taccagagtt gactccgata
    18481 aagttatttt ctgtttgttt tccgatttta accaaatcga gacgctctag ttttaaatca
    18541 aagagatgct ctgccacgac tcctgtcaag atctccaagg atgctgaaga agacaagcca
    18601 gaaccatttg ggatattccc ataaacataa aaatcaaaac ctttgtcaat cacgtgccca
    18661 gcttcttgca agaaatgaag aacccctttt ggatagttgg tccagttgtg ctctttttca
    18721 aacttgaggt cagcgagagg cacttcaatt atgcccttgt cctcaaagtt agctgagtag
    18781 aaacgcaaga cttggtcatc acgcttgcga gctgcaccat aagttcctaa ggaaatagca
    18841 gctggaaaaa cgtgcccacc gttgtagtct gtgtgttcac caatcaaatt aatacggcct
    18901 ggtgaaaaga aagtttggtc tgcttcttga ccaaaaacag caagaaagtc tttgcgaaga
    18961 gtttcagcag taagatgttg tgtcatatga tttctccttt gactgtccgt taagccacct
    19021 taacgtgtaa tcgttttctt ctattgtatc caagggattt cccaaggtca atccttttta
    19081 ctaaaatttt actaaactat cagtaaaaag cagaaaaata tgatatacta acctaatcaa
    19141 ggaggattta tggctacctt aaaagacatt gcacagctag cctctgtctc tatcgcgacc
    19201 gtatcccgcg tcctcaaccg cgaccagagc ctatctgtta cagaagaaac cagacaccgt
    19261 attttaaccg ttgctgaaga gctgggctac accaagcacc tcaagacagg cgagtcccat
    19321 aaacccaagc aaaagattgc cattattcaa tgggtcagcg aacaagggga gctggacgac
    19381 ctctactact accagattcg cctaggaata gaaaaaagag cccaagagtt ggactatgat
    19441 atcttgcgct attttaatga ccatcctttt accttaagcg aggaagtgat tgggattctc
    19501 tgcatcggaa aatttagccg agctcagatt tctgcctttg aagaatacca aaaacccctg
    19561 gtctttctag acagtgatac actttccctg ggacatacct gcattattac ggacttttac
    19621 actgccataa aacaggttgt cgattatttc ctcagccaag gaatggaccg tatcgggatt
    19681 ctaacaggcc ttgaagaaac aactgaccaa gaagaaatca ttgaggataa gcggctggaa
    19741 aatttcagaa actacagtca agcaaaagga atctatcatg atgaactgat ctttcaagga
    19801 aactttactg cccagtctgg ctatgacttg atgaaggaag ccattcagaa cttgggagac
    19861 caactcccac cagcattttt cgcagccagc gatagtttag ccatcggtgc ccttcgtgcg
    19921 ctccaagaag ctggaatcag cctaccagac cgtgtcagcc tcatttcctt taacgacacc
    19981 agcctgacca agcaggttta tcctcccctc tccagcatca cagtttatac tgaagaaatg
    20041 gggcgagcag gtatggatat tcttaacaag gaagtcctcc acggtcggaa aatccctagc
    20101 ctgaccatgc tgggaaccag actgacatta agagaaagta ccctaaatca agaataggat
    20161 aacataaaaa acgaatagag ttctaaaact cctattcgtt ttttattcga ttacaatcat
    20221 agacttaatt gtcttacgtt catccatatc tttataggct tggtcgatat cttccagttt
    20281 ataacttgaa gtaaagacgc gacctggatt gatatcacca tcaaggacgg cttttagtaa
    20341 aaattgctta tcgtatgttg tagcagaagc tgccccacct gctacagaga tattttgcat
    20401 aaatgtcgaa ccaagagcac gattattata gtgtgggact cctacaaagc ccatacgccc
    20461 tccattatga agaacaccta gcgcctgttc tatagcagcc tccgtaccaa cacattcaag
    20521 tgctgcatct gctcctccac cgaggatttc acgcaccttg gtaattcctt cttgaccacg
    20581 ttctgcaaca acagctgtcg cacctgactc catagccatc ttttgacggt cttcatgacg
    20641 gctcataagg ataatttgtg atgctccacg catcttagcc gcgatgacag cacattgacc
    20701 aacagcccca tcaccgataa caacaacctt gtcccctttt tgaacatttg caacacgcgc
    20761 cgcatgatag cctgtcggca tgacatctgc aagagtcaaa agggacttga gcatcccttc
    20821 tgtatagtca gaaggttgac cagggatttt aaccagcgcc cagtttgcat agtggaagcg
    20881 aatatattct gcctgaaaat caccccccaa attattgcca atatgattgt cgcaagaacc
    20941 gtcaaatcca gcaagacagg catcacactc accacatcca tgtgtaaaag ggacaatcac
    21001 aaaatcacct ggtttcaccg tcgtaatggc ttccccagct tcttcaacaa tcccaatcgc
    21061 ttcgtgtcca ctatttttgt gtccagcttt cgtttctgga ttacggtacc tccataaatc
    21121 tgaaccacaa acgcacgcac gaaccacacg aataatcaca tcatccgctt ctattatttg
    21181 cggacgttca atgctagcaa gtccaacctg acctgccttt gtatatactg ctgatttcat
    21241 ttaaaatttt ccttccttat aaagtttaat tttgagattt aaacgattta aagctgtctg
    21301 tagctgagac aagcgctcct ctaatttttg cttttcctct tctaaaatac caagcctctc
    21361 ctctctcgtt tcatctccct tttggtagag cgacatatag tcaactaaac tatctacaga
    21421 gacacccgcc gaacgaaaac acttaataaa ttccagcgct tcgatatcct gatcttgaaa
    21481 atcacgaatc ccagtagcag tacgagtaat cggtggcaca agaccaaccc gttcataata
    21541 ccgaatcgta tccgctgaaa ttcccaacaa atcactggca gatttaatat tcacacgcta
    21601 acctcctctc atttcattca ctgaattcac gctattctaa ctcacttatc ttattatata
    21661 actttgagtt gactccaagt caagcatttt gtatctaatc gaaaaatttt tatcattaac
    21721 aatcatatat ctggagttta tacaatgctc ttctcttacc aatttttgaa atatttaaat
    21781 aataaagaaa ttactctatt ccaaataaga tagtacccaa gtatttttca cttttttcta
    21841 atgttgatgc tcataactcc gttccaagtc acacaccttt cgcttatggg tttggtgagt
    21901 ttctaggtca gcatcaattt caatggtaat attttgaaaa ccacaatctt ttaggaaaat
    21961 tcgaatagac tctttacaag tttccatatg ttccatttct tttagacaaa catgtacaat
    22021 ggcatttttt tccaaagcat ccatagtcca gagattaagc tgattaaggc tggccacatt
    22081 gtccaatcgc tccaggccac tctttacttg cttgatatca agaccttctg gcacagcatc
    22141 caagaaaatc ttgagtgtag accaaaaacg tggaagggct tttgaaagaa taaagaaaga
    22201 aatgacaagg gacaaaagag gatctaggat ataccagtcc gtaaatcgaa gaacaatcgc
    22261 catcaggata acagctaccc accctagcgt atcttccaga aaatgcaaac tcagaataga
    22321 ctcattcttt gtctttccct taccaaccac cagactcgct aacagattga tagtaatcgc
    22381 aataattcct aaccagagaa tcccctcatc attgactggt tgcggatgca aaatcttcgt
    22441 gacattttcc aaaatgacta gaacagagcc cgttacgaga atcacagctg ttaccaaggc
    22501 tcctagcagg ctaaaccgct tatagcccaa ggtgtactga ttgtcttctt cacgattgga
    22561 gattgtttct agaaaagctg atattccaat tgcaatcgca tctcccaagt catgcacaga
    22621 gtcagcaaga acagcgctag aaccaaatac tccacctgca ataaactcaa caatggcata
    22681 agtcaaattt aagaaaaaag ccacccaaac agcatatttt gccttcatat ttctcattcc
    22741 tttgttataa tagatttatg aacaccttgt tcattatcat tatccactaa aactcaaaga
    22801 aaggatagaa gcaaaacgtc agctttattc agttctgaac aatttgcctc aagtgtccat
    22861 atgactaaca ttgaccgccg tatcagcaaa accaaaaaag ccatctatca agcttttata
    22921 caacttttga atgctaagga ctacgagacc actactgttc aggatatcat tgatctcgca
    22981 gatgtgggac gatccacctt ttactgtcac tatgagagta aagagcttct tctggatcag
    23041 ctctgccgct acctcttcca tcatcttttt gaaagagagc aagccatttc aactgaggac
    23101 tacctcgccc acctctttct ccattttcat aaaaaccaag accatatcac cagtctgcta
    23161 ttttccaaaa atgactactt cctccgtcaa ctccagagag agcttgagca ccacgtctat
    23221 tccgtgctag ctgataattt aaaagaagcc caccctaatc tgcctacttc ttacctccaa
    23281 cacttggtca tgtccaactt tatcgagaca ttgacctggt ggctcaaaaa aggtcaagat
    23341 ttcacagacc aggaagttgt ccaattttat ctagaccttc tcattcctaa aaattgaata
    23401 tagagtaaag ctcagttgtc ttatttctag gttactgagt tttttatctt ttcaacaaca
    23461 aaagaggacc cgccgatcct ctttttcata ctataaatcc ttgattatca actatatctg
    23521 ttttaatcga aatctcaaaa cagcactttc aaacatcttt tcctagttaa gtaaatcagt
    23581 attctgctta gctgccttgc tccattgata ccaaccaact agactgttaa tgagataaat
    23641 tagatatttc ccttgaattt gcaggctttc tccccaccag agatagattg aaaaaacatt
    23701 ggtagccgcc cagaatatcc actgttcacg gtaaacagct gtcatgagga tttgccctac
    23761 cccattggtt gcatctgtga ttgaatcacg atagggacga ttggcaccaa tagactgata
    23821 aatgaagcca aaggccaacc accaaagcac actaatggaa agatactttg tccagccctt
    23881 tccgtccagt ttacgcgcga caaactcctg cttttccttc ttaaactgtg cctgataaat
    23941 ccaaactaga agtccaattg gctgcatgac tgtgaagtaa agtgtcgtca gcacctcacc
    24001 ataaaagcct ttctgtaggg ccaaaataag gtaaataaca gagttaatca agccaaaaag
    24061 ataattactt gctcgacctt ccgatacaaa gattacacag ataatccctg tcaagctaca
    24121 aatcatccca atccagtcaa caatacgatg ttcgtaaacc aactccagcc agagaggaaa
    24181 acttcctaaa accagcaaat aaatccactg ggcaaaacta cgatgggcaa agaggtcatc
    24241 ccagatagcc ttcatagttc ctgaaaatcc taaatcagcc atagccgcaa ccatacgacg
    24301 gtaaccacct gacatttcac ctagggttgt tttgatattt tcaattttct tttgcaaata
    24361 agtatgcatc atttctcctt ttgtttttaa agagccgtgt ctggatagac tttcggacgc
    24421 aacgctctat tagataatga actgcctata cacaagattt ctaaccttag tcgacatgag
    24481 ctgaaacctc ttatttgtta agtagttcac aaaatattat acacctattt tatgaatagt
    24541 caactgtctt tacagtaaaa ttttagaaaa tcatgaaaat tttctctttc tttccatttt
    24601 aagtgacatt cagtcattct cacatcaaaa aag
//
LOCUS       DBERDK010000024        24614 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.024, whole genome shotgun sequence.
ACCESSION   DBERDK010000024 DBERDK010000000
VERSION     DBERDK010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 24614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24614
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.024"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            357..1520
                     /locus_tag="ACL73L_000995"
     CDS             357..1520
                     /locus_tag="ACL73L_000995"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HGS0771992.1"
                     /translation="MNRIRVSKRVEKKLAKGLVLLEASDLENVNLKDQEVEVQGQEGN
                     FLGTAYLSQQNKGLGWFVSKDKVAFNQAFFETLFRKAKEKRSAYYQDDLTTTYRLFNQ
                     EGDGFGGLTVDLYGNYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGLDY
                     ESAHVYGQEAPDFFTVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLAMGKSLLN
                     MFSYTAAFSVAAAMGGASQTTSVDLAKRSRELSQAHFQANGLSTDEHRFIVMDVFEYF
                     KYAKRKDLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAA
                     NVSRQKFTEQIDKGFAGRSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            1517..2194
                     /gene="aroD"
                     /locus_tag="ACL73L_000996"
     CDS             1517..2194
                     /gene="aroD"
                     /locus_tag="ACL73L_000996"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HGS0771993.1"
                     /translation="MKLIVSVMPRSLEEAQALDATRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQLYQPDYIDFEYYSYKD
                     VFEEMLDFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEVSAPGQISLASMKKI
                     REILDEA"
     gene            2184..3038
                     /locus_tag="ACL73L_000997"
     CDS             2184..3038
                     /locus_tag="ACL73L_000997"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HGS0771994.1"
                     /translation="MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGAYVAWEI
                     EASDLAETVANIRRYQMFGINLSMPYKEQVIPYLDELSDEARLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRPYLDKLQEQTGFKVDLCALEYVSELQARIAESDLLVNATSVGMDGQSSPVPENIVL
                     PETLLVADIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIW
                     QSLTEKYQ"
     gene            3057..4124
                     /gene="aroB"
                     /locus_tag="ACL73L_000998"
     CDS             3057..4124
                     /gene="aroB"
                     /locus_tag="ACL73L_000998"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HGS0771995.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTGLNGSVESILEHAETLI
                     EHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     VAEEKGLMPAGITQSITEMCQKFGLPVDYENWKVDKLYQALTHDKKARGNTLKLVLVP
                     ELGSATIHPVSLEEMKDYLVK"
     gene            4134..5300
                     /gene="aroC"
                     /locus_tag="ACL73L_000999"
     CDS             4134..5300
                     /gene="aroC"
                     /locus_tag="ACL73L_000999"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HGS0771996.1"
                     /translation="MRYLTAGESHGPRLTAIIEGIPAGLPLTAEDINEDLRRRQGGYG
                     RGGRMKIESDQVVFTSGVRHGKTTGAPITMDVINKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLAELDMEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY"
     gene            5310..6413
                     /locus_tag="ACL73L_001000"
     CDS             5310..6413
                     /locus_tag="ACL73L_001000"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HGS0771997.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADVIILSLPIKQTIAFIKELANLDLREGVIISDAGSTKST
                     IVDAAEQYLAGKSVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSQDTLKE
                     MKDLLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNSEIILDRIEDFKERLEAIGQAISKGDEEQIWNFFN
                     QAREQRQAMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNAQDLERAEHLITENTDYTVVVK"
     gene            6424..6762
                     /locus_tag="ACL73L_001001"
     CDS             6424..6762
                     /locus_tag="ACL73L_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HGS0771998.1"
                     /translation="MSNIYDSANELSRGLRELPEYKAVKAAKDAIAADAEASKIFTDY
                     LAFQEEIQKLAQTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
     gene            6854..8137
                     /gene="aroA"
                     /locus_tag="ACL73L_001002"
     CDS             6854..8137
                     /gene="aroA"
                     /locus_tag="ACL73L_001002"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HGS0771999.1"
                     /translation="MKLKTNISHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVVTIQGVGMDGLKAPQNALDMGNSGTSIRLISGVL
                     AGADFEVEMFGDDSLSKRPMDRVTIPLKKMGVSISGQTERDLPPLHLKGTKKLAPIQY
                     ELPIASAQVKSALMFAALQAQGESVIIEKECTRNHTEDMLQQFGAHLSVDGKKITVRG
                     LQKLTGQKVVVPGDISSAAFWLVAGLIVPNSRLVLQNVGINETRTGIIDVIRAMGGKL
                     EITEIDPVAKSATLIVESSDLKGTEIGGALIPRLIDELPIIALLATQAQGVTVIKDAE
                     ELKVKETDRIQVVADALNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAI
                     AALLVADGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            8130..8606
                     /locus_tag="ACL73L_001003"
     CDS             8130..8606
                     /locus_tag="ACL73L_001003"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HGS0772000.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDPNYLDMDALIEKRLGMSIANFFA
                     EKGEAAFRHVESEVLADLLQTDQVVSTGGGVVISQRNRDLLKTNTDNIYLKADFETLY
                     LRIAADKDNQRPLFLNNSKEELVAIFQERQAWYEEVASRVLDVTKLSPEEIIEELR"
     gene            8603..9451
                     /gene="pheA"
                     /locus_tag="ACL73L_001004"
     CDS             8603..9451
                     /gene="pheA"
                     /locus_tag="ACL73L_001004"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HGS0772001.1"
                     /translation="MKIAYLGPKGSFSHHVVQTAFPHEELQAFANITDVIKAYEQGLV
                     NYSVVPVENSIEGSVHETLDYLFHQAHIQAVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYPEAQIEVTASTAYAARFISEHPDQPFAAVAPRSSAEEYGLELIA
                     EDIQEMEANFTRFWVLGAEKPSIPLQAQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESRPLKTALGEYFFIIDVDYTDKDLVHFAQKELEAIGIQYKILGAYPIYPISD
                     HGKERR"
     gene            9448..10722
                     /gene="psr"
                     /locus_tag="ACL73L_001005"
     CDS             9448..10722
                     /gene="psr"
                     /locus_tag="ACL73L_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HGS0772002.1"
                     /translation="MSKENPLSHHEQLRYDYLLKNIHYLNEREKNEFAYLQEKLTLAR
                     GNSSSSLEQEREEQVDLPSYANRSRSQSKSQALSFPPKKKRRKLRLKRIFMVIFSLLV
                     CVALAMVFMFLRGYQDASAKKTADARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFDLDIKYYALVDFQAFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSTLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLAQMGLR"
     gene            11001..11846
                     /locus_tag="ACL73L_001006"
     CDS             11001..11846
                     /locus_tag="ACL73L_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HGS0772003.1"
                     /translation="MNNMTDLKAIQARSLEMAEYFVAFCKEHDLLCYLCGGGAIGALR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSHKDFVDRNLFITIRDKET
                     TCIKPYQQDLDLPHGLALDILPLDYYPKNPAERKKQVRWALIYSLFCAQTIPEKHGAL
                     MKWGSRILLGLTPKSLRYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EDNLFLPFEGTEMPIPIGYDVYLRTAFGDYMTPPPADKQVPHHDAVIADMDKSYTEYK
                     GEYGG"
     gene            11836..12939
                     /locus_tag="ACL73L_001007"
     CDS             11836..12939
                     /locus_tag="ACL73L_001007"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGS0772004.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDPEKYDIDILEME
                     HFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIYFPRLTRRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTSNS
                     IKEVYPDYTSKLQTIYNGYDFQTILEKSQEKIDIEIAPKSICTIGRIEENKGSDRVVE
                     VIRLLHQEGKNYHLYFIGAGDMEEELKKRVKEYGIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTDVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSASNFDVDEASQFIQQFTITKQIEQVEKLLEE"
     gene            12941..13927
                     /locus_tag="ACL73L_001008"
     CDS             12941..13927
                     /locus_tag="ACL73L_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGS0772005.1"
                     /translation="METALISVIVPVYNVAQYLEKSIASIQKQTYQNLEIILVDDGAT
                     DESGRLCDSIAEQDDRVSVLHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQ
                     SLYEQLVQEDADVSSCGVMNVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTI
                     CNKLIKRQIATALSFPKGLIYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKP
                     YAEKDLAYIDIYQKFYNEVVKNYPDLKEVAFFRLAYAHFFILDKMLLDDQYKQFEAYS
                     QIHRFLKGHAFAISRNPIFRKGRRISALALFINISLYRFLLLKNIEKSKKLH"
     gene            13943..15376
                     /locus_tag="ACL73L_001009"
     CDS             13943..15376
                     /locus_tag="ACL73L_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HGS0772006.1"
                     /translation="MIDGKRLLFSLTIVSYALTLVSGIVYLFNNNNVSLLSTLLFLLV
                     SSLIACWNDIKYYLIHFIFYLTIFVFLVSRPTIDYFRDGALDTYHPIAYRFAFIVVMI
                     SILGLTTGGILARYFIARKKIKVANIGNSLKEVYIKRLRFVSLGVFLLTYPFYFIRLF
                     ERLLYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYLATKPKKLQATAVLVSFI
                     AANTIHLAIGTRNPFILSILFAFVYYFMREQTEKGKWIGFKEKLAIFVGSPILMLAMG
                     VLNYVRDNVQVSHTGFWDILLDFIYKQGTSFGVLARGFLFNSSLPYRDFRNFTFGPVL
                     DYFARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLVLNKEYLKGHGIGSSYIM
                     ELYTDYGMIGVFLLSFLLGVLFIAMLQVAYRSRTILFALSLLILNNLFFMPRSSFSES
                     FFNLFTMQFWGIVLVIIFVAKMLTKEN"
     gene            15405..16439
                     /locus_tag="ACL73L_001010"
     CDS             15405..16439
                     /locus_tag="ACL73L_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HGS0772007.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPKESAKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVEP
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDANDLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYLFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            16639..17376
                     /gene="mecA"
                     /locus_tag="ACL73L_001011"
     CDS             16639..17376
                     /gene="mecA"
                     /locus_tag="ACL73L_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HGS0772008.1"
                     /translation="MKMKQISDTTLKITMSLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFEDLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSLEQVEAKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVAFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFADDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
     gene            17527..18813
                     /locus_tag="ACL73L_001012"
     CDS             17527..18813
                     /locus_tag="ACL73L_001012"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HGS0772009.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNRLLAAGNDFNFVTNVDDILSDQDITIVVELMGRIEPAKTFITRALEAGKHVV
                     TANKDLLAVHGAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKITRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFAESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVAHKGIRNITPEDVAVAQELGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILALDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKARV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            18815..19684
                     /gene="thrB"
                     /locus_tag="ACL73L_001013"
     CDS             18815..19684
                     /gene="thrB"
                     /locus_tag="ACL73L_001013"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HGS0772010.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHD
                     LRVDTQGVRVEAK"
     gene            19760..19912
                     /locus_tag="ACL73L_001014"
     CDS             19760..19912
                     /locus_tag="ACL73L_001014"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772011.1"
                     /translation="MKIKEQTRKLAAGCSKQCFEVVDRTDDVSSRHCFEVVDRTDEVS
                     NIYTVR"
     gene            19979..20917
                     /gene="msrB"
                     /locus_tag="ACL73L_001015"
     CDS             19979..20917
                     /gene="msrB"
                     /locus_tag="ACL73L_001015"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HGS0772012.1"
                     /translation="MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLI
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKASLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
     gene            20975..22699
                     /locus_tag="ACL73L_001016"
     CDS             20975..22699
                     /locus_tag="ACL73L_001016"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0772013.1"
                     /translation="MKHLLSYFKPYIKESILAPLFKLLEAVFELLVPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGFAKELTNDLYRHILSLSKD
                     SRDRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISAELTFWFL
                     VMVAILTIVIVGLSRLVNPLYSSLRKKTDQLVQETRQQLQGMRVIRAFGQEKRELQIF
                     QTLNQVYARLQEKTGFWSSLLTPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLINSLNQSYISAKRIEEVFAESPENIHSELEQKQVTSGRVLQV
                     QELTFTYPDAAQPSLRDISFDMTQGQILGIIGGTGSGKSSLVQLLLGLYPVDKGNIDL
                     YQNGRSPLNLEQWRSWIAYVPQKVELFKGTIRSNLTLGFNQEVSDQELWQALEIAQAK
                     DFVSEKEGLLDALVEAGGRNFSGGQKQRLSIARAVLRQAPFLILDDATSALDTITESK
                     LLKAIRENFPNTSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYCEIN
                     ASQHGKED"
     gene            22701..24449
                     /locus_tag="ACL73L_001017"
     CDS             22701..24449
                     /locus_tag="ACL73L_001017"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0772014.1"
                     /translation="MKRQTVNQTLKRLAVDLASHPFLLFLAFLGTIAQVGLSIYLPIL
                     IGRVIDQVLVAGSSPVFWQIFLQMLLVVIGNTLVQWANPLLYNRLIFSYTRDLRERII
                     HKLHRLPIAFVDRQGSGEMVSRVTTDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKRSYHLFQKQTETRGIQTQLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYSSTVNPSTRFVNALIYALLAGVGAYRIMMGST
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSPEVAETGKEVLT
                     SDQVKRAISFKHVSFGYHPEKILIKDLSIDIPAGSKVAIVGPTGAGKSTLINLLMRFY
                     PISSGDILLDGQSIYDYTRVSLRQQFGMVLQETWLTQGTIHDNIAFGNPEASREQVIA
                     AAKAANADFFIQQFPQGYDTKLENAGESLSVGQAQLLTIARVFLAIPKILILDEATSS
                     IDTRTEVLVQDAFAKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIVEYGNHQELMDR
                     KGKYYQMQKAAAFSSE"
BASE COUNT         7218 a         4602 c         5572 g         7222 t
ORIGIN      
        1 taacaaaagg cttctctata aaatcaaatt ttccagatat ctactgaagc tgatttattt
       61 attcatcttt cttctactac ctttgtcact aaatttgaca gacacatatt gctgatacaa
      121 cctctctacc tataatatca cagttaacct gaggctagtt tcatagtttg ctatttgatt
      181 ttcgttgata aaaaaaatgc tcctccaaga cggaagagca ttttttattg tgattcttac
      241 acatttactt cctagttttg caggaagtct agtataccat tttttaaaag aagagtaaaa
      301 aagcaagggt agtttctttt ttgagaattg tctaaaagtt tgatataatg gtagttatga
      361 atagaataag agtcagcaaa agggttgaaa agaagcttgc taaggggcta gttttactag
      421 aagccagtga tcttgagaat gtcaatctta aggatcagga agtagaggtg cagggtcagg
      481 aaggaaactt tcttgggact gcctaccttt ctcagcaaaa caagggcttg ggctggtttg
      541 tcagcaaaga caaggtggcc ttcaatcaag ctttctttga aacgttgttt agaaaagcca
      601 aagaaaagag aagcgcctac tatcaagatg atttaacaac tacctatcgt ctctttaatc
      661 aagagggaga tggctttggg ggtctgacag tggaccttta tggcaactac gccgtctttt
      721 cttggtataa ctcttatgtt tatcagattc gtcagactat atcagaagcc tttagacagg
      781 ttttccctga ggttttaggg gcttatgaga aaatccgctt taaaggtttg gactatgaat
      841 ctgcccatgt ttatggtcaa gaagcacctg actttttcac tgttttagaa aatggtgtcc
      901 tgtatcaagt ctttatgaat gatggtttga tgacaggaat tttcctagac cagcatgagg
      961 ttcgcggtag tttagttgac ggcttggcta tgggtaaatc cttgctcaat atgttttcct
     1021 acacagcggc tttttcagta gctgcggcca tgggaggagc tagccagaca acttctgttg
     1081 atctagccaa acgttcacga gaattgtctc aagcgcattt tcaggcaaat gggctcagca
     1141 cagacgagca tcgttttata gtcatggatg tctttgagta tttcaaatat gccaaacgca
     1201 aagacttgac ctacgatgtg attgtcctag atccgcctag ctttgctcgg aataaaaaac
     1261 aaactttctc tgtggccaag gattatcaca agttgatttc ccagagtctt gagattttaa
     1321 atccgggagg gattatcatt gccagtacca atgctgccaa tgtttcccgc cagaaattta
     1381 cagaacaaat tgataaaggc tttgcaggaa gaagttacca gattttaaac aaatatggtc
     1441 ttccagcaga ttttgcctat aataaaaaag atgaaagtag taattacctc aaggtgatta
     1501 gtatgaaggt tagtaaatga aattaatcgt ttcagtaatg ccaagaagtt tagaggaggc
     1561 tcaggctctg gatgccacga ggtacctgga tgccgacatc attgaatggc gtgccgacta
     1621 tctgcctaaa gaagcgattt tgcaggtagc tccagccatt tttgaaaaat tcgcaggccg
     1681 tgagttggtt ttcacgctac gaactcgctc cgaaggtgga gaaatcgacc tttctccaga
     1741 agagtatatc catctaatca aggaagttgc gcaactctat caaccagact atattgattt
     1801 tgagtactat agctacaagg atgtttttga ggaaatgctg gacttcccaa atcttgtttt
     1861 gagttaccat aatttccaag aaacacctga gaacatgatg gaaatcttgt cagagttgac
     1921 gatcctaaat ccaaaacttg ttaaggttgc ggtgatggct cacacggagc aggatgtctt
     1981 agacttgatg aactatacac gaggctttaa aaccctcaat cctgaacagg aatatgtgac
     2041 catttctatg ggtaaggtgg gcaaggtctc tcgtatcact gcggatgtga ctggttcgag
     2101 ttggtctttt gccagtctgg atgaggttag tgcccctggg cagatttctc tagctagcat
     2161 gaaaaaaatc agggagattt tggatgaagc ttgatggcta tacacgttta gctgcagttg
     2221 ttgccaatcc tattaagcat tctatttctc ccttcatcca caatagagcc tttgaggcga
     2281 cagctaccaa cggtgcttat gtggcttggg agattgaagc gagtgacttg gcagaaacag
     2341 tggccaatat tcgtcgctac cagatgtttg gcatcaatct gtccatgccc tataaggagc
     2401 aggtgattcc ttatttggat gagctaagcg atgaagcgcg cttgattggt gcggttaata
     2461 cggttgtcaa tgagaatggc aatttaattg gatataatac agatggcaag ggatttttta
     2521 agtgcttgcc ttcttttaca atttcaggta aaaagatgac cctgctgggt gcaggtggtg
     2581 cggctaaatc aatcttggca caggctattt tggatggcgt cagtcagatt tcggtctttg
     2641 ttcgttccgt ttctatggaa aaaacaagac cttacctaga caagttacag gagcagacag
     2701 gctttaaagt ggatttgtgt gctttagaat atgtttctga actgcaagca aggattgccg
     2761 agtcggattt gctagttaat gccaccagtg tgggcatgga tggccaatcc tctcctgttc
     2821 ctgaaaacat agtcttacca gaaactctct tagttgcaga tatcatatac caaccctttg
     2881 aaacaccgtt tttgaaatgg gctagaagtc agggcaatcc agcagtcaat ggtctgggaa
     2941 tgttgctcta tcaagctgca gaagcttttc aactgtggac aggcaaggaa atgccgacag
     3001 aagagatttg gcagtcttta acagaaaaat accaataaag aaagggagac tctcctatga
     3061 aaatcagaat cgatattcct catcatcctt atgatattca gattgaaaaa ggttgtatgg
     3121 cccaggctgg tcagtggttg cgagaactct ggcaaccgca aaaggtagtc attgtgacag
     3181 ataaccatgt agcctctctc tatgcagaga aggtcaagct cagcctagaa gatgctggtt
     3241 ttcaggtagc tgtttttgat ttcttagaag gtgaagaaag aaagaattta actactgttc
     3301 agaaagtcta tgaatttcta gtcaagcaag gtctgactcg tagcgatgga atcgttgctc
     3361 ttggtggtgg cgttgttggg gacctggctg gctttgtagc ctctacctat atgcggggta
     3421 ttcactttgt tcagattccg actagtttga cagctcaggt tgattcttct atcggtggaa
     3481 agacaggcgt taacacacca tttgctaaaa atatggtggg gacctttgcc caaccagatg
     3541 gggttctgat tgatccactt gttcttgaaa ccctcggaaa aagagagttg attgaaggga
     3601 tgggtgaagt tatcaagtat ggcttgattg aggatccaga actgtgggct ctcttgacgg
     3661 gactgaatgg ttctgttgag agtattttgg aacatgcaga gaccttgatt gaacattctt
     3721 gtcaggtgaa gcgcaagatg gtggttgaag atgagttgga caatggtatt cgtctttacc
     3781 tcaattttgg ccacactatt ggccatgcca tcgaagcgac tgccggttat ggcaaggtca
     3841 tgcatggaga ggctgttgcc atgggaatgg tacagatttc caaggttgct gaggaaaaag
     3901 gcctcatgcc agctggcatt acccaatcta tcacagagat gtgtcagaaa ttcggcttgc
     3961 ctgttgacta tgaaaattgg aaagttgaca agctttatca ggctcttact catgacaaga
     4021 aagcgcgtgg taacaccttg aaattggtct tggtgccaga gcttggttca gcgaccattc
     4081 acccagtttc tctggaagag atgaaagact acttggtaaa ataaggagag tgtatgagat
     4141 atttaactgc aggagaatca cacggccccc gtctaacagc tattattgag ggaattccag
     4201 ctggacttcc attgacagct gaggatatca atgaggatct tagacgccgt cagggtggct
     4261 acggtcgtgg tggtcgtatg aagattgaga gtgaccaggt tgtctttact tcgggtgttc
     4321 gccacgggaa gacgacaggg gcgcctatta ctatggatgt catcaataag gaccaccaga
     4381 aatggctgga catcatgtct gcggaggaca ttgaagaccg ccttaaaagc aagcggaaaa
     4441 ttactcatcc tcgcccaggt catgccgatt tggttggggg gattaagtac cgttttgatg
     4501 atttgcgaaa ttctttggag cgttcatcag ctcgtgaaac caccatgcgg gtggcagttg
     4561 gtgcagtagc caaacgcctc ttggctgagc tggatatgga gattgccaac catgtcgtgg
     4621 tctttggtgg taaggaaata gatgttcctg aaaatctgac agtcgctgaa attaagcaaa
     4681 gagctgccca gtctgaagtt tctattgtca accaagaacg agaacaggaa atcaaggact
     4741 atattgacca aatcaaacgt gatggtgata ccatcggtgg ggttgtggag acagtcgtcg
     4801 gaggcgttcc agttggtctt ggttcctatg tccaatggga tagaaaattg gatgcaagat
     4861 tggctcaagc tgttgtctct atcaatgcct ttaaaggggt ggaatttggt cttggctttg
     4921 aggctggtta tcgtaaaggc agccaagtta tggatgaaat tctctggtct aaagaagacg
     4981 gttatactcg ccgtaccaat aatctaggtg gttttgaagg tggtatgact aatgggcaac
     5041 ccatcgttgt tcgtggggtc atgaaaccca ttcctactct ttataaacct cttatgagtg
     5101 tggatatcga aacccacgaa ccttacaagg caaccgtgga gagaagtgat ccgactgctc
     5161 ttccagctgc aggaatggtc atggaagcag ttgtagcaac ggttctggcg caagaaatcc
     5221 tcgaaaaatt ctcatcagat aatcttgagg aactaaaaga agcggtagcc aaacaccgag
     5281 actatacaaa gaactattaa ggagttccta tggcaaaaac aatctatatc gcaggtcttg
     5341 ggttgattgg agcctctatg gcacttggta tcaaacgcga tcatccagat tatgaaattt
     5401 taggttataa tcgtagtcaa gcttcgagag atatcgcctt gaaagaaggt atgattgacc
     5461 gtgcaacgga tgattttgct agttttgctc ctttggcaga tgtcattatc ctcagcttgc
     5521 caatcaaaca aactattgct ttcattaagg agttggccaa tttggatttg cgagaaggcg
     5581 ttattatttc agatgctggt tcgaccaagt caaccattgt ggatgcggcg gagcagtatt
     5641 tggctggcaa gtctgttcgc tttgtcgggg cccatcccat ggctggtagt cacaagacag
     5701 gggctgcttc ggcagatgtc aatctttttg aaaatgccta ttatatcttt acaccttcaa
     5761 gcctgacaag tcaggacacg cttaaggaaa tgaaggatct gctttcaggt cttcatgctc
     5821 gttttatcga gattgatgcc aaggagcatg atcgtgtcac ttctcagatt agccattttc
     5881 ctcatatttt ggcttctagt ctcatggagc agactgcggt ctatgctcaa gagcatgaga
     5941 tggcaaggcg ctttgcggca ggtggttttc gagatatgac ccgaattgcg gaaagcgagc
     6001 caggaatgtg gacctccatt ctcttgtcca atagcgagat cattctggat agaattgagg
     6061 atttcaagga acgtttggaa gcgattggtc aggccattag taagggagat gaagagcaaa
     6121 tttggaactt ttttaaccaa gcgcgtgaac aacgtcaggc catggaaatc cataagcgtg
     6181 gaggtgtgga tagttcttac gacctctatg tcgacgttcc cgatgaagaa gatgtcatct
     6241 tgaggatttt ggaactacta cgtggaactt ccttggtcaa tattcatatc aacgaggaaa
     6301 accgtgagga tattcacggg attctacaaa tttcatttaa aaatgctcaa gacttggaaa
     6361 gagctgagca tctcataaca gaaaataccg actacacagt cgttgtcaaa taaggagaaa
     6421 atcatgtcaa atatttacga tagtgcaaac gaacttagtc gcggtctacg cgaattacca
     6481 gaatacaagg ctgtcaaagc agctaaagat gcgattgcag cagatgctga ggcaagcaaa
     6541 atctttacag attatcttgc ctttcaagag gaaattcaaa aactagcaca gacaggtcaa
     6601 atgccagatg cgtccttcca agctaagatg gaaggctttg gcaagcagat tcaaggaaat
     6661 agcctcttgt cagaattctt tactaagcaa caacaattgg ccatttacct ttctgacatt
     6721 gaaaaaattg ttttcgaacc agtttcagaa ttgctaaaat aagaaaataa ctatcctaaa
     6781 gtcaggaatt tttaaggaat ttctggcttt ttatgataaa ataggtaaaa gtattgcaag
     6841 tatgaggtcc agtatgaaac taaaaacaaa cattagccac ttacatggca gtatccgcgt
     6901 cccaggtgac aagtctatca gccaccgttc cattatcttt ggaagtttag ctgagggaga
     6961 gaccaaggtt tatgatattt tgcggggtga agacgtcctt tcaaccatgc aggtctttcg
     7021 tgatcttggt gtagagattg aggataaaga tggggttgtt accattcaag gtgtcggtat
     7081 ggacggccta aaagctccac aaaatgccct tgatatggga aattctggaa cctcgattcg
     7141 cctgatttca ggtgtccttg ctggtgcaga cttcgaagta gaaatgttcg gagacgatag
     7201 tctttccaaa cgtcctatgg accgtgtgac gattccactg aaaaaaatgg gcgttagcat
     7261 ttcagggcaa actgagcgag acttgcctcc ccttcactta aaagggacga aaaaactagc
     7321 acctattcag tatgagttgc caattgcctc tgcccaagtc aagtcagcct tgatgtttgc
     7381 ggccttgcag gctcagggag agtcagtgat tatcgaaaaa gaatgcaccc gtaatcatac
     7441 tgaagatatg ctgcaacaat ttggtgctca tttaagtgtg gatggcaaga aaatcacagt
     7501 ccgaggactt caaaaactga caggacaaaa ggtggttgtt ccaggagata tttccagtgc
     7561 agccttttgg ttggtcgcag gtttgattgt tccaaattct cgtctagtgc tgcagaatgt
     7621 ggggatcaac gaaactcgca ccggtattat tgatgtcatt cgtgccatgg gtggaaaatt
     7681 ggaaataact gaaatcgatc cagtcgctaa atctgctacc ttgattgttg agtcttctga
     7741 cttgaaagga acagagattg gtggcgcttt gattccacgt ttgattgatg aattgcccat
     7801 tattgccctg cttgcgactc aagcccaagg tgtaacagtt atcaaggatg ctgaggagct
     7861 caaggtcaag gaaacagacc gtattcaggt tgtggcagac gccttaaata gtatgggagc
     7921 agatattact cctacggcag atgggatgat tatcaaaggg aaatcagctc ttcacggtgc
     7981 tagagtcaat acgtttggtg accaccgtat cggcatgatg acagctatcg cagccctctt
     8041 ggttgcagat ggagaggtgg agcttgaccg tgcagaagcc atcaatacca gctatcctag
     8101 tttctttgat gatttggaga gcttgattca tggctaaggt gttattaggg tttatggggg
     8161 ctggaaaatc gactattgca agaggcttgg accctaatta ccttgatatg gatgctctga
     8221 ttgagaagcg cctaggtatg tccattgcga attttttcgc tgaaaaggga gaagcagcct
     8281 ttcgtcatgt agaatcagaa gtcctagctg atttactaca aacagaccaa gtcgtgtcaa
     8341 ctggaggagg agtggttatt tctcagagaa atcgtgactt actcaagact aatacagata
     8401 acatctacct gaaagcagat tttgaaaccc tctacctacg tatcgcagct gataaggaca
     8461 atcagcgacc gctttttcta aataatagca aggaagaact agtagctatt tttcaagaaa
     8521 gacaggcttg gtatgaggaa gtggctagtc gggttttgga tgtgaccaag ctaagcccag
     8581 aggaaattat agaggaacta agatgaaaat agcttatcta ggtcccaagg gatcattttc
     8641 acaccacgtt gtgcagacag cttttcctca tgaggaattg caggcttttg ccaacattac
     8701 agatgtcatc aaagcttatg agcaaggatt agtgaactat tctgtggtgc cagttgaaaa
     8761 ttctattgag ggtagtgttc atgaaacctt ggactatctt tttcatcagg ctcacatcca
     8821 agcagtagca gaaatcgttc agcctattca tcagcagttg atggtggttc caggccatac
     8881 taagattgaa aagatttttt cacatccaca ggccttggct caaggaaaga aattcatcga
     8941 tgaacaatat ccagaggctc aaatcgaggt aacagctagt acagcttatg cggcccgttt
     9001 tatttccgaa catccagacc agccctttgc agcagttgca cctagaagtt ctgctgaaga
     9061 atatggattg gaactgattg ccgaggatat tcaggaaatg gaagccaatt tcacacgttt
     9121 ctgggttcta ggagctgaaa agcctagtat tcccttgcaa gcacaaactg aaaagatgag
     9181 tttggccttg acattacctg acaaccttcc aggtgcactt tataaggccc tgtcgacctt
     9241 tgcttggcga ggaattgact tgacaaaaat tgaaagtcgt ccactcaaga cagcactggg
     9301 tgaatacttt ttcattatcg atgtggatta taccgataag gacttggtcc actttgccca
     9361 aaaagaatta gaagcgattg gaatccagta taaaattctg ggtgcctatc ctatttatcc
     9421 aatatcagac catggaaagg agagaagatg agtaaagaaa atcccttaag tcatcatgag
     9481 cagttgcgtt atgattattt gctaaaaaat attcactacc tcaatgagag agaaaaaaat
     9541 gagtttgcct atttgcaaga aaagctaact cttgctaggg gaaatagtag ctctagcttg
     9601 gaacaagaaa gagaagagca ggttgactta ccaagctatg cgaaccggag tcgctcacaa
     9661 tccaaatcac aagcactctc tttccctcca aaaaagaaaa gacggaagct ccgtcttaaa
     9721 cgaattttta tggtgatttt ttcacttttg gtctgtgtgg ctttggccat ggtattcatg
     9781 tttttgcgtg gttaccaaga tgctagcgca aagaaaactg ctgatgcccg ggcagctcaa
     9841 gtagaagtct ttaatggtca ggacactaga gatggagtta atattttaat catgggtact
     9901 gatggtcgaa tcggccagaa cagtgttgag acacgaactg actctattat ggtattaaat
     9961 gtcgggggct cagataagaa aatgaagcta gtcagtttca tgcgtgataa tttggtctat
    10021 atagatggtt atagtcaagt gattaacggt agaaaacaga cagataacaa gttaaacgta
    10081 gcctacgagt taggagaaca agaggggcaa aaaggggcag aaatggttcg ccaagtcttg
    10141 aaagataatt ttgacttgga cattaagtac tacgccttgg ttgatttcca ggcctttgcc
    10201 acagcgattg acacgctttt ccctgatggg gtgacgattg atgctcaatt ttcaacattg
    10261 aatgggcgtc ccctaacaga agctacagtc ggagatgatt tatatgctac cgagactgag
    10321 tctccaaccc aaaccattaa agtcggaaaa cagcagatga atggctcgac cctgctcaat
    10381 tatgctcgtt tccgtgatga tgatgaggcg gattacggcc gtacaaaaag acagcaacaa
    10441 gttttaacag caattttaga gcaaattaaa gatcccacta aacttttcac tggttctgaa
    10501 gctcttggaa aggtttttgc tatgacctca accaacgtac cctatacttt cctcctaaca
    10561 aacggtttat ctgttttgga tggagcaaaa aatggtattg aaaaattgac gattccagaa
    10621 ctgggtgact gggtagatgc ctatgatgtt tatggaggct tgggcttgct ggttgatcaa
    10681 aacaaatatc aaaccaagct cgctcaaatg ggcttgagat aagataatac tcaatgaaaa
    10741 tcaaagtgca aactaggaag ctagccgcag gctgctaaaa acacagtttt gaggttgtag
    10801 ataagactga cgaagtcagt tacctatacc tacggcaagg tgaagctgac gtggtttgaa
    10861 gagattttcg aagagtataa gacagaaaaa tcaaagtttg atagctattt ggatagcagt
    10921 caggctttga ttttttacag tctgcaatag attttcaccc cttgtttgtg gtataatgaa
    10981 acgatacgat agaaagaata atgaacaata tgactgattt aaaagcaatt caggctcgta
    11041 gtctggagat ggctgaatat tttgtggcct tttgtaaaga acatgatttg ctctgttatc
    11101 tctgtggcgg aggcgctatt ggtgcccttc gaaacaaggg atttattcct tgggacgacg
    11161 acctagactt ttttatgcct cgtaaagatt atgagaaatt agcagaatta tggcctcgtt
    11221 atgcagatga acgttatttc ttgtcaaaga gtcacaagga ttttgttgat cgtaatcttt
    11281 ttattaccat tcgtgacaag gaaaccacct gtatcaagcc ttatcagcag gatttggatt
    11341 tgccacatgg tctggccttg gatattttgc ctttggatta ttatccgaaa aatccagctg
    11401 agcggaaaaa acaggttcgt tgggccttga tttattcact cttttgtgcg caaactattc
    11461 cagaaaagca tggtgctctc atgaaatggg gaagtcgcat tttactgggt ttgactccaa
    11521 aatctctccg ttatcgcatc tggaaaaaag ctgagaaaga aatgactaag tatgatttgg
    11581 ctgattgtga tggcattaca gaattatgct caggtcctgg ctacatgaga aacaagtacc
    11641 caatcacatc ttttgaagac aatcttttct tgccatttga aggaacagag atgcctattc
    11701 caatcggcta tgatgtctat ctcagaactg cttttgggga ttatatgacg cctccaccag
    11761 cagacaagca ggtaccgcat catgatgctg tcatcgctga tatggataag tcttatacag
    11821 aatacaaggg agaatatggt ggctaagaaa aaaatcttat tttttatgtg gtctttttct
    11881 cttggaggtg gtgcagagaa gattctatca accattgttt caaatctgga tccagaaaag
    11941 tatgatattg atattcttga aatggagcac tttgacaagg gatatgaatc tgttccaaag
    12001 catgtacgca ttttaaaatc ccttcaagat tatcgccaaa ccagatggtt acgagctttt
    12061 ttgtggagaa tgagaattta ttttccaaga ctgactcgtc gtttgcttgt aaaagatgat
    12121 tatgatgttg aagtttcttt taccattatg aatccaccac tgttgttctc taaaagaaga
    12181 gaagtcaaga agatatcttg gattcatgga agtattgaag aacttcttaa ggatagctct
    12241 aaaagagaat cacatagaag ccagttggat gctgcgaata caattgtagg gatttcaaaa
    12301 aagaccagca attctatcaa ggaagtttat ccagattata cttctaaatt acagacaatc
    12361 tacaatggat atgattttca gactattcta gaaaaatctc aagagaagat cgatatcgag
    12421 attgctccta aaagtatctg tactatcgga cggattgagg aaaataaggg ttctgaccgt
    12481 gtagtggaag tgatacgatt attacaccaa gagggaaaaa actatcatct ctattttatc
    12541 ggggctggtg atatggaaga ggaactgaaa aaacgagtca aagagtatgg gattgaggac
    12601 tatgtacatt tccttggtta tcaaaaaaat ccttatcagt atctatctca gacgaaagtt
    12661 cttttgtcta tgtctaaaca agaaggtttt cctggagtgt atgtggaggc cttgagtctg
    12721 ggactccctt ttatctctac ggacgttgga ggggctgagg aattatccca agaaggacga
    12781 tttggacaaa tcattgagag caatcaagag gcagctcagg cgattactaa ttacatgact
    12841 tctgcctcaa actttgatgt cgatgaggct agccaattca ttcaacaatt tacaattaca
    12901 aaacaaatcg aacaagtaga aaaactatta gaggagtagc atggaaactg cattaattag
    12961 tgtgattgtg ccagtctata atgtggcgca gtacctagaa aaatcgatag cttccattca
    13021 gaagcagacc tatcaaaatc tggaaattat tcttgttgat gatggtgcaa cagatgaaag
    13081 tggtcgcttg tgtgattcaa tcgctgaaca agatgacagg gtgtcagtgc ttcataaaaa
    13141 gaacgaagga ttgtcgcaag cacgaaatga tgggatgaag caggctcacg gggattatct
    13201 gatttttatt gactcagatg attatatcca tccagaaatg attcagagct tatatgagca
    13261 attagttcaa gaagatgcgg atgtttcgag ctgtggtgtc atgaatgtct atgctaatga
    13321 tgaaagccca cagtcagcca atcaggatga ctattttgtc tgtgattctc aaacatttct
    13381 aaaggaatac ctcataggtg aaaaaatacc tgggacgatt tgcaataagc taatcaagag
    13441 acagattgca actgccctat cctttcctaa ggggttgatt tacgaagatg cctattacca
    13501 ttttgattta atcaagttgg ccaagaagta tgtggttaat actaaaccct attattacta
    13561 tttccataga ggggatagta ttacgaccaa accctatgca gagaaggatt tagcctatat
    13621 tgatatctac caaaagtttt ataatgaagt tgtgaaaaac tatcctgact tgaaagaggt
    13681 cgcttttttc agattggcct atgcccactt ctttattctg gataagatgt tgctagatga
    13741 tcagtataaa cagtttgaag cctattctca gattcatcgt tttttaaaag gccatgcctt
    13801 tgctatttct aggaatccaa ttttccgtaa ggggagaaga attagtgctt tggccctatt
    13861 cataaatatt tccttatatc gattcttatt actgaaaaat attgaaaaat ctaaaaaatt
    13921 acattagaac gggggtgacc aagtgattga tgggaaacga ttattattta gtttgaccat
    13981 agtcagttat gccttgacgc tagtaagtgg aattgtgtat ctgtttaata ataataatgt
    14041 tagcttactt tctactttat tgttcttgtt ggttagtagc ttaattgctt gttggaatga
    14101 tatcaagtat tacttaatcc attttatttt ctatttaacc atttttgtat ttctggtatc
    14161 aagaccgacc attgattatt ttagggatgg tgctttggat acctatcatc caatagccta
    14221 tcgttttgcc tttatagttg tcatgatttc gattctgggc ttgaccacag gaggcattct
    14281 ggctcgttac ttcatagcta ggaagaaaat aaaagtagca aatataggaa attctctaaa
    14341 agaggtttat atcaagcggt tacgctttgt atcactagga gtttttcttc taacttatcc
    14401 tttctatttc attcggttat ttgaacggct cttgtatcgt ttgcagactt cctactatgc
    14461 ctactatgca aattttgaaa gtaaactgcc ttattttacc tacattttgt ctacctttac
    14521 ggtctatgca atgtgtatgt atctggcaac caagccaaag aaattgcagg ccacagcagt
    14581 gcttgtctcc tttattgcag ctaatactat tcatttggca attgggacac gaaatccctt
    14641 tattttaagt attttatttg cttttgttta ttactttatg cgggagcaaa ctgaaaaagg
    14701 aaaatggatt gggtttaaag aaaagttagc gatttttgta ggttctccta ttctcatgtt
    14761 agcgatggga gtactcaatt atgtacggga taatgtccaa gtttcccata caggtttctg
    14821 ggatatctta cttgacttta tctataaaca agggactagt tttggtgttc tggctcgagg
    14881 ttttctattt aacagtagcc tcccttaccg agatttccgt aattttactt ttggtcctgt
    14941 tcttgattat tttgcaaggg ggagtttggg agccattttc ggaggaaaag cctttgaaca
    15001 tacaaccaat agtgtggaac tagctattga tagtaatagt tatgcccaca atctatccta
    15061 tcttgtcttg aacaaggaat acttgaaagg gcatggtatc ggaagtagtt atatcatgga
    15121 gttgtatacc gactatggta tgattggagt ctttctgctt agtttcttac tcggcgtatt
    15181 atttatagcc atgctgcaag tagcctatcg ctcaaggaca atcctatttg ctttatccct
    15241 actcatcttg aataatctat tctttatgcc aagaagcagc ttttcagaaa gtttcttcaa
    15301 tttatttaca atgcaattct ggggaattgt tcttgtgatt atatttgtag caaaaatgct
    15361 tacaaaggaa aactagtatc tactaaacaa aggagaaaaa aatcatgttt gaacattatt
    15421 cagtagctga tttgtttgca aatctttata aaaagcgaaa agcaaatatt ttagctctca
    15481 tcgctttatt tgctctcatt gctgtaccat ttacaattaa agcagttagg aataaaaaca
    15541 ctgtcaaaga tacaacaagt tattcaactt atcttatcta taaaatcact cctccaaaag
    15601 agtcggccaa aacgattttg aatcatcaaa ttggtggtta tagtgatttt tatgggaaat
    15661 tgattgatgg taatttgaat ggagcttatc ttttcaatga tgtagaaccc agtgagttga
    15721 aaaaaattgc cagtgaatta gacacgacag aaacaacctt gaaaaattca acgaatgact
    15781 attggtggaa aaaattgacc gtctactata tgattgacga tgcaggggtt ggtgtgaaaa
    15841 ttttgacatc aagtaaagat gccaatgact tgttagagaa aaaaattgat gggttgattg
    15901 agaaatttaa acatgcctat gcaaatgtga aaattgaaaa actggaaacc atcaactcta
    15961 aagaattgaa cgcaaatggt gaaacagcgc ttggcttaaa tgtgaaaaat ctgattcttc
    16021 gtttagttgt tattggagtg gtttgtgtga ttttggttgt gatgggaaat gtgttagttt
    16081 atctctttaa tccaacaatc aatagagtag gtgatttttc tcagtatcaa attgattttg
    16141 taacagagat tacaacaatt gctaacctag cagatgtttt gtcatacaaa aatactggac
    16201 aggaattgac catcgttagc tcaaataaag ctatcttaga taaattgaaa cagagtcaag
    16261 aagctttaaa aggaatgcat tttgtagatt tacaggatgt atcatctctt ttggaaagag
    16321 atacagtcct tcttgttgaa gagtacggag tgactcgtta taagaaattt gagcaaagtc
    16381 ttcaaattct cagaaactta aatcgttcta tcctcggtgt agcaaccttt aaattataag
    16441 tattctgatg tataaggtct tcaaaatcgg ctcttagccg attttgaagg ctttttctat
    16501 ctgtctgcag aaattttctt ttggagtctc ctatagaaag accctaggga aaatctatcc
    16561 gtatagagga gccttctttt gataaaatcg taaaaatcta gtataataga tagaactgaa
    16621 agtatgaggt tactagcaat gaaaatgaaa caaattagtg atacaacttt aaaaatcacg
    16681 atgtctttag aggatttgat ggatcgtgga atggagattg ctgactttct cgttcctcaa
    16741 gaaaaaacag aagagttctt ttatgctatc ttggatgagc tagagatgcc tgatagcttt
    16801 ctggatacag gcatgttgag cttccgtgtg actccaaaac ctgataaggt agatgtcttt
    16861 gtgaccaagt caaagattga ccaaaatcta gattttgaag acttatcgga tttgccagat
    16921 atggaagaat tggctcaaat gtctccagat gaatttatca aaaccctgga aaaaagcatc
    16981 gcagacaaaa ccaaggatga tatcgaagcc attcaatctc ttgagcaagt tgaagccaag
    17041 gaagaagagc aagagcaagc tgaacaagaa gctgagagta agaaagaacc ttacatctac
    17101 tacatccttt cttttgctaa gttggctgac ttggtagctt ttgccaagac agtgactttt
    17161 gaaatggaaa cttctgaact ctacaaaatg aacgagcgct attatttgac cattttagtg
    17221 gatattgaaa atcatccaag cccatatcca gcttggctgt tggcccgtat gcgcgagttt
    17281 gcagacgata gtgatatcag tcgctcagtc ttacaagagt atggtcaagt cttgatgagt
    17341 cacgatgcag tgctcaatct gcaaaaaatc ggctaatcct ttctggaaag ctattttcag
    17401 attttaagaa gagcgaatcg tctgattcgt tttttctttt tatactgaaa tagtgattta
    17461 ctataatagg aattttcaca aaattctgtt ataatggcta tattagaaaa tttcgaggag
    17521 ataaagatga cagttaaaat tgctttacta ggatttggta ccgttgcaag tggtgtgcct
    17581 ttcctcctaa aggaaaatgg aggaaaaatc aatcaatcag cacattcaga tatcaaagtt
    17641 gctaaggtat tggtcaagga tgaagatgaa aaaaatcgct tgcttgcagc agggaatgac
    17701 tttaactttg taaccaatgt ggatgatatt ttatcagacc aggatattac tatcgtagtg
    17761 gaattgatgg ggcgtattga gcctgctaaa acctttatca ctcgtgcctt ggaagctgga
    17821 aaacacgttg ttactgctaa caaggacctt ttagctgtcc atggcgcaga attgctagaa
    17881 atcgctcaag ctaacaaggt agcactttac tacgaagcag cagttgctgg tgggattcca
    17941 attcttcgta ctttagcaaa ttccttggct tctgataaaa ttacgcgcgt gcttggagta
    18001 gtcaacggaa cttccaactt catggtgacc aagatggtgg aagaaggctg gtcttacgat
    18061 gatgctcttg cggaagcaca acgtctagga tttgcagaaa gcgatccgac gaatgacgta
    18121 gatgggattg atgcagccta caagatggtt attttgagcc aatttgcctt tggcatgaag
    18181 attgcctttg atgatgtagc ccacaaggga atccgcaata tcacaccaga agacgtagct
    18241 gtagctcaag agcttggtta cgtagtgaaa ttggttggtt ctattgagga aacttcttca
    18301 ggtattgctg cagaagtgac tccaaccttc ctacctaaag cgcacccact tgctagtgtg
    18361 aatggcgtaa tgaacgctgt ctttgtagaa tctatcggta ttggtgagtc tatgtactac
    18421 ggaccaggtg ctggtcaaaa accaactgca acaagtgttg tagctgatat tgtccgtatc
    18481 gttcgtcgtt tgaatgatgg tactattggc aaagacttca acgaatatag ccgtgacttg
    18541 gtcttggcaa atcctgaaga tgtcaaagca aactactatt tctcaatctt ggctctagac
    18601 tcaaaaggtc aggtcttgaa gttggctgaa atcttcaatg ctcaagatat ttcctttaag
    18661 caaatccttc aagatggcaa agagggtgac aaggcgcgtg tcgttatcat cacacacaag
    18721 attaataaag cccagcttga aaatgtctca gctgaattga agaaggtttc agaattcgac
    18781 cttttgaata ccttcaaggt gctaggagaa taagatgaag attattgtac ctgcaaccag
    18841 tgccaatatc gggccaggtt ttgactcggt cggtgtagct gtaaccaagt atcttcaaat
    18901 tgaggtctgc gaagaacgag atgagtggct gattgaacac cagattggca aatggattcc
    18961 acatgatgag cgtaatctct tgctcaaaat cgctttgcaa attgtaccag acttgcaacc
    19021 aagacgcttg aaaatgacca gtgatgtccc tttggcgcgc ggtttgggtt cttccagctc
    19081 ggttatcgtt gctgggattg aactagccaa ccaactgggt caactcaact tatcagacca
    19141 tgaaaaattg cagttagcga ccaagattga agggcatcct gacaatgtgg ctccagccat
    19201 ttatggtaat ctcgttattg caagttctgt tgaagggcaa gtctctgcta tcgtagcaga
    19261 ctttccagag tgtgattttc tagcttacat tccaaactat gaattacgta ctcgcgacag
    19321 ccgtagtgtc ttgcctaaaa aattatctta taaggaagct gttgctgcaa gttctatcgc
    19381 caatgtagcg gttgctgcct tgttggcagg agacatggtg accgctgggc aagcaatcga
    19441 gggagacctc ttccatgagc gctatcgtca ggacttggta agagaatttg cgatgattaa
    19501 gcaagtgacc aaagaaaatg gggcctatgc aacctacctt tctggtgctg ggccgacagt
    19561 tatggttctg gcttctcatg acaagatgcc aacaattaag gcagaattgg aaaagcaacc
    19621 tttcaaagga aaactgcatg acttgagagt tgatacccaa ggtgtccgtg tagaagcaaa
    19681 ataaagaata gaagatagga tggggaaact cttgaccaga gggtttcata tcctttttgt
    19741 gaaaagaagt ttatactcaa tgaaaatcaa agagcaaact aggaagctag ccgcaggctg
    19801 ctcaaaacag tgttttgagg ttgtggatag aactgacgac gtcagctcaa gacactgttt
    19861 tgaggttgtg gatagaactg acgaagtcag taacatatat acggtaaggt gacgctgacg
    19921 tggtttgaag agattttcga agagtattag ttaaaaacgt gataaaggag aaataaagat
    19981 ggcagaaatt tatctagcag gtggttgttt ttggggccta gaggaatatt tttcacgcat
    20041 ttctggagtg ctagaaacca gtgttggcta cgctaatggt caagtcgaaa cgaccaatta
    20101 ccagttgatc aaggaaacag accatgcaga aacggtccaa gtgatttacg atgagaagga
    20161 agtgtcactc agagagattt tactttatta tttccgagtt atcgatcctc tatctatcaa
    20221 tcaacaaggg aatgaccgtg gtcgccaata tcgaactggg atttattatc aggatgaagc
    20281 agatttgcca gctatctaca cagtggtaca ggagcaggaa cgcatgctgg gtcgaaagat
    20341 tgcagtagaa gtggagcaat tacgccacta catcctggct gaagactacc accaagatta
    20401 tctcaggaag aatccttcag gttactgtca tatcgatgtg accgatgctg ataagccatt
    20461 gattgatgca gcaaactatg aaaagcctag tcaagaggtg ttgaaggcca gtctatctga
    20521 agagtcttat cgtgtcacac aagaagctgc tacagaggct ccatttacca atgcctatga
    20581 ccaaaccttt gaagagggga tttatgtaga tattacgaca ggtgagccac tcttttttgc
    20641 caaggataag tttgcttcag gttgtggttg gccaagtttt agccgtccga tttccaaaga
    20701 gttgattcat tattacaagg atctgagcca tggaatggag cgaattgaag ttcgttctcg
    20761 ttcaggcagt gctcacttgg gtcatgtttt cacagatgga ccgcgggagt taggcggcct
    20821 ccgttactgt atcaattctg cttctttacg ctttgtggcc aaggatgaga tggaaaaagc
    20881 aggatatggc tatctattgc cttacttaaa caaataaaac agagagtggg gcttcccact
    20941 ttcttcattt ctagaatatg aatagaaggg atttatgaaa cacctattat cttacttcaa
    21001 accctacatc aaggaatcaa ttttagcccc cttgttcaag ctgttagaag ctgtttttga
    21061 gctcttggtt cccatggtga ttgctgggat tgttgaccaa tctttacctc agggagatca
    21121 aggtcatctc tggatgcaga ttggcctgct ccttatcttt gcagtaattg gcgttttagt
    21181 ggccttgata gctcaatttt actcagcaaa ggcagcagta ggctttgcta aggaattgac
    21241 aaacgatctt tatcgtcata ttctttcctt gtccaaggac agcagagacc gtctgacaac
    21301 ttctagtttg gtcactcgct tgacttcgga tacctaccag attcagactg gtatcaatca
    21361 attcctgcgt ctctttttgc gagcgcccat tatcgttttt ggtgccattt ttatggctta
    21421 tcgaatctca gctgagttga ctttctggtt cttagtcatg gttgccattt tgaccattgt
    21481 cattgtaggg ttatctcgat tggtcaatcc tctctacagt agtctcagaa agaaaacgga
    21541 ccaactggtt caggaaacgc gccagcaatt gcaagggatg cgggttattc gtgcttttgg
    21601 tcaagaaaaa cgagagttac agatttttca aacccttaac caagtttatg ctagattaca
    21661 agaaaagaca ggtttctggt ctagtttatt aacacctctg acctatctga ttgtcaatgg
    21721 aactcttctc gttattatct ggcaaggcta tatttcaatt caaggaggag tgctcagtca
    21781 aggtgctctc attgctctta tcaattacct cttacagatt ttggtggaat tggtcaagct
    21841 tgccatgctg atcaattccc tcaaccagtc ctatatctca gccaagagaa tcgaggaggt
    21901 ctttgcggaa tctcccgaaa acatccattc agaattagaa caaaagcaag ttaccagtgg
    21961 tcgggtttta caagtccaag aattgacttt tacctatcct gatgcggccc agccttctct
    22021 gagagacatt tcctttgata tgactcaagg acaaatcctt ggtatcattg gggggactgg
    22081 ttctggtaaa tcaagcttgg tgcaactctt acttggactt tatccagtag acaaggggaa
    22141 cattgacctt tatcaaaatg gacgtagtcc tcttaatttg gagcagtggc ggtcttggat
    22201 tgcctatgta cctcaaaagg tcgaactctt taaaggaacc attcgttcca acttgactct
    22261 aggtttcaat caagaagtat ctgaccagga actctggcag gccttggaga ttgcgcaagc
    22321 taaggatttt gtcagtgaaa aggaaggact cttggatgct ctagttgagg caggggggcg
    22381 aaatttctca ggtggacaaa aacaaagatt gtctatcgcc cgagcagtct tgcgccaagc
    22441 tccgtttctc atcctagatg atgcaacctc ggcactggat accattacag agtccaagct
    22501 cttgaaagct attagagaaa attttccaaa cacgagctta attttgatct ctcaacgaac
    22561 ctcaacttta cagatggcgg accagattct cctcttggaa aaaggtgagt tgctagctgt
    22621 tggcaagcac gatgacttga tgaaatccag ccaagtctat tgtgaaatca atgcatccca
    22681 acatggaaag gaggactaga atgaaacgac aaactgtaaa ccagacgctc aaacgtttag
    22741 ccgtagattt agcaagccat cctttcctcc ttttcctagc ctttctagga actattgccc
    22801 aagttggctt atcaatttac ctacctattc tgattgggcg ggtcattgac caagtcctag
    22861 tggctggttc atcaccagtt ttttggcaga tttttctcca gatgctcttg gtggtaatag
    22921 gaaatactct agtacaatgg gccaatcctc tcctctataa tcgtctaatc ttctcttata
    22981 ccagagattt acgggagcga atcatccata aactccatcg gttaccgatt gcctttgtag
    23041 ataggcaagg tagtggagag atggttagtc gtgtaaccac agacatcgaa cagttggcag
    23101 ctggcttgac catgattttt aaccaatttt tcattggtgt tttgatgatt ttggttagta
    23161 ttctagccat gctccaaatt catctcctca tgactctctt agtcttgctg ttgacgccac
    23221 tgtccatggt gatttcacgc tttattgcca agagatccta tcatctcttc cagaagcaaa
    23281 cagagacgag ggggattcag acccagttga ttgaagaatc gcttagccag cagactataa
    23341 tccagtcctt caatgctcaa acagagttta tccaaagatt gcgtgaggct catgacaact
    23401 actcaggcta ttctcagtca gccatctttt attcttcaac ggtcaatcct tcgactcgct
    23461 ttgtaaatgc actcatttat gcccttttag ctggagtagg agcttatcgt atcatgatgg
    23521 gttcaacctt gaccgtcggt cgtttagtga cttttttgaa ctatgttcag caatacacca
    23581 agccctttaa cgatatttct tcagtgctag ctgagttgca aagtgctctg gcttgcgtag
    23641 agcgtatcta tggagtctta gatagccctg aagtggctga aacaggtaag gaagtcttga
    23701 ccagtgacca agttaagaga gctatttcct ttaaacatgt ctcttttggc taccatcctg
    23761 aaaaaatttt gattaaggac ttgtctatcg atattccagc tggtagtaag gtagccatcg
    23821 ttggtccgac aggtgctgga aaatcaactc ttatcaatct ccttatgcgt ttttatccca
    23881 ttagctcggg agatatcttg ctggatgggc aatccattta tgattataca cgagtatcat
    23941 tgagacagca gtttggtatg gtgcttcaag aaacctggct cacacaaggg accattcatg
    24001 ataatattgc ctttggcaat cctgaagcca gtcgagagca agtaattgct gctgccaaag
    24061 cagctaatgc agactttttc atccaacagt ttccacaggg atacgatacc aagttggaaa
    24121 atgctggaga atctctctct gtcggccaag ctcagctctt gaccatagcc cgagtctttc
    24181 tggctattcc aaagattctt atcttagacg aggcaacttc ttccattgat acacggacag
    24241 aagtgctggt acaggatgcc tttgcaaaac tcatgaaggg ccgcacaagc ttcatcattg
    24301 ctcaccgttt gtcaaccatt caggatgcgg atttaattct tgtcttagta gatggtgata
    24361 ttgttgaata tggtaaccat caagaactca tggatagaaa gggtaagtat taccaaatgc
    24421 aaaaagctgc ggcttttagt tctgaataag cgattctctt ttgaaagttt atggacgaaa
    24481 aaagttgcct tcgagtgact tttttgttac aatagctaga aaaattgttc actgtaatac
    24541 tcaatgaaaa tcaaagagca aactaggaag ctagccgtag gttgctcaaa gcacagcttt
    24601 gaggttgtag ataa
//
LOCUS       DBERDK010000025        23782 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.025, whole genome shotgun sequence.
ACCESSION   DBERDK010000025 DBERDK010000000
VERSION     DBERDK010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 23782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23782
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.025"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(97..2355)
                     /locus_tag="ACL73L_001018"
     CDS             complement(97..2355)
                     /locus_tag="ACL73L_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGS0772015.1"
                     /translation="MLCQNCKINDSTIHLYTNLNGKQKQIDLCQNCYKIIKTDPNNSL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRPSSNTPPIPPTQSGGGYGGNGGYGSQNRGSAQ
                     TPPPSQEKGLLEEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGDVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNILKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILKGIQKKYEDYHHVQYTDAAIEAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPIISEKTIEHIIEQKTNIPVGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGFA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKDAIIIMTSNAGTGKTEASVGFGAAREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADVNKRLSSNNIRLDVTDKV
                     KEKLVDLGYDPKMGARPLRRTIQDYIEDTITDYYLENPSEKDLKAVMTSKGNIQIKSA
                     KKAEVKSSEKEK"
     gene            complement(2584..2814)
                     /locus_tag="ACL73L_001019"
     CDS             complement(2584..2814)
                     /locus_tag="ACL73L_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1797 family protein"
                     /protein_id="HGS0772016.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVAYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
     gene            3004..3687
                     /locus_tag="ACL73L_001020"
     CDS             3004..3687
                     /locus_tag="ACL73L_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGS0772017.1"
                     /translation="MNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQRS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQVGILGVDLSRLIPGILII
                     SMNSGAYVSETVRAGINAVPKGQLEAAYSLGIRPKNAMRYVILPQAIKNILPALGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KRMGQGDKK"
     gene            3687..4421
                     /locus_tag="ACL73L_001021"
     CDS             3687..4421
                     /locus_tag="ACL73L_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGS0772018.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGINLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKVIFEGVDITDKKNDLFAMREKMGMVFQQFNLFPNMTVMENI
                     TLSPIKTKGESKAVAEKRAQELLEKVGLPDKADAYPQSLSGGQQQRIAIARGLAMEPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQEQRTKDFLSKVL"
     gene            4575..5432
                     /locus_tag="ACL73L_001022"
     CDS             4575..5432
                     /locus_tag="ACL73L_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HGS0772019.1"
                     /translation="MTQIIDGKALAAKLQGQLAEKTEKLKEETGLVPGLVVILVGDNP
                     ASQVYVRNKERSALAAGFRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPK
                     HIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIDLEGKN
                     AVVIGRSNIVGKPMAQLLLAKNATVTLTHSRTHNLSKVAAKADILVVAIGRAKFVTAD
                     FVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASHITPVPGGVGPMTITMLMEQTYQ
                     AALRTLDRK"
     gene            5575..6447
                     /locus_tag="ACL73L_001023"
     CDS             5575..6447
                     /locus_tag="ACL73L_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HGS0772020.1"
                     /translation="MKVINQTLLEKVIIERSRSSHKGDYGRLLLLGGTYPYGGAIIMA
                     ALAAVKSGAGLVTVGTDRENIPDLHSHLPEPMAFSLQDQQLLKEQLEKAEVILLGPGL
                     GDNAFGEDLVKQVFAGLKQNQILIVDGGALTILARTSLSFPSSQLILTPHQKEWEKLS
                     GITIEKQKEDATASVLTSFPQGTILVEKGPATRIWEVGQSDYYQLQVGGPYQATGGMG
                     DTLAGMIAGFVGQFRQASLYERVAVATHLHSAIAQELSQENYVVLPTEISRYLSKIMK
                     IICQQDRVSKDKLV"
     gene            6729..7412
                     /gene="rpiA"
                     /locus_tag="ACL73L_001024"
     CDS             6729..7412
                     /gene="rpiA"
                     /locus_tag="ACL73L_001024"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HGS0772021.1"
                     /translation="MENLKKMAGIKAAEFVSDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQITAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRHFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQMVDKVIVAGRDGV
                     QISTSKKGK"
     gene            7426..8637
                     /locus_tag="ACL73L_001025"
     CDS             7426..8637
                     /locus_tag="ACL73L_001025"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019318858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HGS0772022.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     TVGLNVPNMAKIGLGNIPRETPLKTVAAESNPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFSPTVLDKLNEAGIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPAFKGNGLIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            8639..9181
                     /locus_tag="ACL73L_001026"
     CDS             8639..9181
                     /locus_tag="ACL73L_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HGS0772023.1"
                     /translation="MTRYALLVRGINVGGKNKVVMAELRQELTNLGLEKVESYINSGN
                     IFFTSIDSKAQLVEKLETFFAVYYPFIQSFSLLSLEDFEAELENLPAWWSRDLARKDF
                     LFYTEGLDVDQVIATVESLELKDEVLYFGKLGIFWGKFSEESYSKTAYHKYLLKVPFY
                     RHITIRNAKTFDKIGQMLKK"
     gene            9197..10006
                     /locus_tag="ACL73L_001027"
     CDS             9197..10006
                     /locus_tag="ACL73L_001027"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HGS0772024.1"
                     /translation="MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVV
                     VDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIGFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRAYTP
                     EYRATAHEVAKKLNIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVA
                     AHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGDFKGLLKAILAEL"
     gene            10496..10891
                     /locus_tag="ACL73L_001028"
     CDS             10496..10891
                     /locus_tag="ACL73L_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HGS0772025.1"
                     /translation="MLDSKEELKESENDAPKLETPLREEPRLAPQTLPEASEVLENKR
                     EESKVEITEPAQADDIRKVVGELAKDISITKLYMTGHSLGGYLAQIAAVEDYQKYPDF
                     YNHVLRKVTTFSAPKVITSRTVWDAKNGF"
     gene            10901..11476
                     /locus_tag="ACL73L_001029"
     CDS             10901..11476
                     /locus_tag="ACL73L_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000445853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HGS0772026.1"
                     /translation="MESRKLAVSGKIKHYVVDNDNVVTPLIHNNRDIVTFTGNSRFKH
                     RSRGYFESPMNDIPNFNIGKQATLDKHGYRDPKLDKVRFFKKQALPQSSSQPSAEPME
                     NIALGKQVTQSSTAFGGDARRAVDGKVDGNYGHNSVTHTNFQSKPWWQVDLAKEETIR
                     QINIYNRTDTAQDRLANFDVILLDSSGKEIE"
     gene            11501..12013
                     /locus_tag="ACL73L_001030"
     CDS             11501..12013
                     /locus_tag="ACL73L_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-activated cytolysin C-terminal
                     domain-containing protein"
                     /protein_id="HGS0772027.1"
                     /translation="MSAQITINHKKARYVRIELEGYNALSLAEVEVFRFIATNAETAT
                     QVSKPVQPISQTPVKDKTLTIQHSGAYIARYSITWEEVPVDKDGNQVVRSHSWEGNGC
                     NQTAGFVLNLPIKENMRNLRVKIEKKTGLLWNRWQTIYENRPILAQPHRKITHWGTTL
                     NSKVSDDDVL"
     gene            12669..13379
                     /gene="deoD"
                     /locus_tag="ACL73L_001031"
     CDS             12669..13379
                     /gene="deoD"
                     /locus_tag="ACL73L_001031"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HGS0772028.1"
                     /translation="MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLDDAVCFNEVRN
                     MFGYTGTYKGHCVSVMGTGMGMPSISIYARELIVDYGVKKLIRVGTAGSLNEEVHVRE
                     LVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKKLGMTTHVGNVLSSDVFY
                     SNYFEKNIELGKWGVKAVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEER
                     QNTFTDMMKVGLETLIAE"
     gene            complement(13402..13926)
                     /locus_tag="ACL73L_001032"
     CDS             complement(13402..13926)
                     /locus_tag="ACL73L_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HGS0772029.1"
                     /translation="MKIAIIGYSGSGKSTIAEKLSNYYSIPKLHMDTLQFQPGLQDSD
                     CEWMLTEIKNFLTKYKAWVIDGNYSWCYYQERMQEADQIIFLNFSPLTCLFRAFKRYL
                     KYRGKVRESMAVDCPERFDWEFIRWILWDGRSKTQKENYQKLCQEYSHKVTILRNQRE
                     LDQFLDKKRKSYNS"
     gene            complement(13979..14215)
                     /gene="rpsT"
                     /locus_tag="ACL73L_001033"
     CDS             complement(13979..14215)
                     /gene="rpsT"
                     /locus_tag="ACL73L_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HGS0772030.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSAKLVK"
     gene            complement(14283..15203)
                     /gene="coaA"
                     /locus_tag="ACL73L_001034"
     CDS             complement(14283..15203)
                     /gene="coaA"
                     /locus_tag="ACL73L_001034"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HGS0772031.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEVYDIVPEEKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDPDSYYYRFTQMPIGEVESFAHQVWISINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
     gene            15393..15796
                     /locus_tag="ACL73L_001035"
                     /pseudo
     CDS             15393..15796
                     /locus_tag="ACL73L_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698118.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            16003..16593
                     /locus_tag="ACL73L_001036"
     CDS             16003..16593
                     /locus_tag="ACL73L_001036"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGS0772032.1"
                     /translation="MSKMYYAENPDAAHDIHELRVDLLGEKMTFLTDAGVFSKKMVDF
                     GSQLLLKCLDVNQGETVLDVGCGYGPLGLSLVKVYGVQATMVDINTRALDLAQRNAEK
                     NNAKATIFQSNIYARVQGRFDHVISNPPIRAGKQVVHEIIEKSKDFLEIGGDLTIVIQ
                     KKQGAPSAKSKMEDVFGNCEILKKDKGYYILRSVKE"
     gene            16590..17867
                     /locus_tag="ACL73L_001037"
     CDS             16590..17867
                     /locus_tag="ACL73L_001037"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HGS0772033.1"
                     /translation="MRAVDLIQKKRDGQELTSSEIEWLIEGYVSGTVPDYQMSAFAMA
                     VYFKGMTTREISDLTMNMVKTGQEFDLSAIDGVKVDKHSTGGVGDKVTLILAPLVASF
                     GVPVAKMSGRGLGHTGGTIDKLESIKGYQVERSQEDFIRQVQDIGVSVIGQSDQLVKA
                     DKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEARELA
                     QTMVDLGKVVGRKTVAVITDMSQPLGRAIGNRLEILEALEILQGQGRQDITHFICELA
                     QIMLGLANVNKTVEEVRQHLENGQALAKFEEMVQAQGGDLEDLYRPVNVAHVVEIPAQ
                     ETGVISALPAMDFGLYAMRLGAGRAVKSDALDYETGIVFEKKIGDSVQKGEIVAKVYT
                     NEKIPPQLVTDFQKCVKISDEVKKIREIVEIIS"
     gene            17885..18547
                     /gene="deoC"
                     /locus_tag="ACL73L_001038"
     CDS             17885..18547
                     /gene="deoC"
                     /locus_tag="ACL73L_001038"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HGS0772034.1"
                     /translation="MKLNKYIDHTLLKQDAKKKQIDSLLSEAREYDFASVCVNPTWVE
                     HAKKGLEGTDVKVCTVVGFPLGATTSAVKAFETKEAIQNGADEIDMVINVGDLKSGNL
                     ALVESDIRAVVEASGDKLVKVIIEACLLTDQEKIVVCQLAQKAGADFVKTSTGFSTGG
                     ATIADVTLMRETVGSDMGVKAAGGARSYADALAFVEAGATRIGTSAGVAILKGELADG
                     DY"
     gene            18534..18923
                     /locus_tag="ACL73L_001039"
     CDS             18534..18923
                     /locus_tag="ACL73L_001039"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HGS0772035.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGNVYTGVNIE
                     NVSYPLTNCGERTAIFKAISEGQREFSELIVYGQTEKPISPCGACRQVMVEFFEQDLK
                     VTLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            19014..20066
                     /locus_tag="ACL73L_001040"
     CDS             19014..20066
                     /locus_tag="ACL73L_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HGS0772036.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQAWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALHNAVEEAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDIANKAEKGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVPEK"
     gene            20210..21745
                     /locus_tag="ACL73L_001041"
     CDS             20210..21745
                     /locus_tag="ACL73L_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0772037.1"
                     /translation="MAHENVIEMRDITKVFGGFVANDKINLHLRKGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVAENI
                     ILGSELTKNGVLDIAGASKEIKALSERYGLAVDPSAKVADISVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEIRAVSDRVTVIRRG
                     KSIETVEIAGATNADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SVGHVPEDRHRDGLILEMMISENIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RAASELVPAAALSGGNQQKAIIAREIDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQELGVLMAGGNLGKEK
                     SDV"
     gene            21738..22796
                     /locus_tag="ACL73L_001042"
     CDS             21738..22796
                     /locus_tag="ACL73L_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS0772038.1"
                     /translation="MSKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGLGFAVASRAGFFNVGLPGQALAGWILSGWFALSHPDM
                     PRPLMILATIVIALIAGGIVGAIPGILRAYLGTSEVIVTIMMNYIVLYVGNAFIHAFP
                     KDFMQSTDSTIRVGANATYQTPWLAELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGAVEGLGTFQNVYVQGSSLAI
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            22799..23755
                     /locus_tag="ACL73L_001043"
     CDS             22799..23755
                     /locus_tag="ACL73L_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS0772039.1"
                     /translation="MSIITLLPLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGSVFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPVIGDIFFKSTSLLGYL
                     AIAFSFLAWFILFKTQFGLRLRSVGEHPQAADTLGINVYKMRYLGIIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPAVYLQIAPYVLTILVLAAFFGKAVAPKADGINYIKSK"
BASE COUNT         6914 a         4308 c         5405 g         7155 t
ORIGIN      
        1 tcataatgga caaagggcaa gctgtcaaga aattacagtt attttagtaa aaaaagaagc
       61 agaaaaattt cattttctac tccttttcta taggatttat ttttcttttt cagaactttt
      121 aacttcagct tttttggcag atttaatctg aatgtttccc ttgctagtca taactgcttt
      181 gagatctttt tcgcttggat tttcaaggta gtagtcagtg attgtgtcct caatatagtc
      241 ttgaatagtc cgacgaagtg ggcgtgctcc catttttgga tcataaccta ggtcaaccaa
      301 cttttccttg accttgtcag ttacatccaa acgaatgttg ttgctagaga ggcgcttgtt
      361 aacatctgct agcatgagct cgacaatctg aaggaggtta tccttgctga gagccttaaa
      421 ttcgataatg ccatcaaaac ggttcataaa ctctgggcta aagaagttac cgagttcacc
      481 gaggacagaa ttggtacgtc cttctctagc agcaccaaat ccaacgctag cttcggtctt
      541 tcctgtacct gcatttgagg tcatgataat gatggcatcc ttgaagctaa cggtgcgtcc
      601 ttgcccgtct gtcaaacgac catcgtccaa gacttgaaga aacatgtgca taacatctgg
      661 gtgagctttt tccacttcat cgagaaggat gagagaatat ggattgtggc gaactttttc
      721 agttaattga ccagcctcat catagccaac ataacctgga ggggcgccga ccaacttagc
      781 cacactatgt ttttccatgt attcactcat atcaaagcga atcatactat cagcaaaacc
      841 aaaaagttcg atagccagtt gtttggaaag ttctgtctta ccgacaccag ttggcccaac
      901 gaagaggaag cttccgattg ggcggttagg ggtaccaagt ccgacacgat tacggcgaat
      961 agccttggca atcttatcga ctgcatcatc ttggccaata acatgagact tgagatcttc
     1021 ggctagatgg atgagttgag attgttcttt ctctttcaaa tcaccaacag ggatattggt
     1081 tttctgctcg ataatgtgct caatagtttt ctcgctgatg ataggagtat cctggtctgt
     1141 gatctttttc ttttgcattt ccttatactt ggcaatctgg tcgcggaagt aggccgcctt
     1201 ctcaaaatct tcttctcgtg tagcttgaga cttgagattt tcagcctcaa tcaagcgctg
     1261 atcaattact ttaggatcca caaaattcaa ggtcaagttc atcttagaac cagcttcatc
     1321 taggaggtca atggccttgt caggcaagaa gcgatcttgg atgtagcgat tggaaagagt
     1381 tgcagctgct tcaatcgcag catctgtata ttgaacgtgg tggtaatctt cgtatttctt
     1441 ttgaatccct ttgagaatag tgattgtttc gtccaccgtt ggttcatcga ctttaacagg
     1501 ctgcatacga cgctcgaggg cagcatcctt ttcaatgata cggtattcat tgagggtagt
     1561 agcaccgact agttgcagtt ctccacgagc aagggctggc ttgaggatat ttcctgcgtc
     1621 catattacca tcactcgcag aaccagcacc aacaatttca tggatttcat cgataaagag
     1681 gatgatgtct tcacgtttgc gaatttcttc catgagtttt tgcatgcgtt cttcaaattg
     1741 tcctcgaatc cccgttcctt gaactaagct aaccacatcc agacggatga cttgtttacc
     1801 ttggagttta tgtggcacat cgccatcgac aattttctga gctagacctt cgacaacggc
     1861 cgtttttccg acaccaggtt caccgataag gacaggatta ttcttggttc tacgattgag
     1921 aatctcgatg acacggataa tctcatcgtc gcgcccaata acggggtcaa tgtctccacg
     1981 acgggcaatt tcagttacat taataccaaa ttcttccagc aggccttttt cttggctagg
     2041 tggcggagtt tgagcagatc cacgattttg ggaaccataa ccgccgtttc caccgtaacc
     2101 tccacctgat tgggttgggg gaataggagg agtattgcta gaaggtctga aattgtttag
     2161 atcattgaag aaatcaccaa agggatcgaa gtcacgattg ttcagatccg tcataccttt
     2221 gaagaggcta ttgttaggat ctgttttgat aatcttatag cagttttgac agaggtcaat
     2281 ttgtttttgt tttccattga gattggtgta aagatgaatt gttgagtcgt taattttaca
     2341 gttttgacaa agcatacatc tacctcattt ctttctttag cctgacttgt ttaaaggtca
     2401 aaaatagtca aattctatac tctcattata acaggattgg ggtgtaaagc aagtaaaaag
     2461 attgaagctt tgagaagttg ttacaatgaa aatttttgtg aatttggact ataaaaaaat
     2521 cctatttgtg agacaaaaag gattttactt agacatgcag ggacaatccc agcttgtttt
     2581 agattagtag agaagttcgt aaatttccat tgcgattaag tcaatgctat caaacgtata
     2641 cgtttcgcga tcttctacgt cacgaagggt aaagattggg ccgttttctt cgtcgtggct
     2701 gtacgcaact tctgcaacaa caactccttc gcgttcaaaa ttacgtttta aatttccgcc
     2761 atcgtttgcc attgcttcaa ggcggttgat gattctaacc aaatgtgatt ccattttgat
     2821 tctccttcat ttcttatcgt tactatttta ccataaagga ggccaacttg actagaaaaa
     2881 actaagattt ctcgctaatt ctgctagttt aaagattttt aaataaattg gataaaaaat
     2941 ttaaatttta attcaaaaca tgttataatc atacagtatt ctattttaga aagcagtgtg
     3001 actatgaatt tttctttttt acctaagtat ttaccttatt ttaactatgg ggctgttgtg
     3061 acgattctta tttctatctg tgttatcttt ttgggaacta ttttgggtgt tgtcttggct
     3121 tttgggcaac gttcaaagtt taaaccgctt gtttggttgg ccaacttgta cgtttggatt
     3181 ttccgtggga caccgatgat ggttcaaatt atgattgcct ttgctcttat gcatatcaat
     3241 gctccgacta ttcaggttgg aattttaggt gttgatcttt ctcgtctgat tccagggatt
     3301 ttgattatct ctatgaacag tggtgcttat gtttctgaga ctgttcgtgc cggaatcaat
     3361 gcagttccta aggggcagtt agaggcggct tattctttag ggattcgtcc taaaaatgct
     3421 atgcgctatg taattttacc acaagctatc aaaaatatct tgccagcttt ggggaacgaa
     3481 tttatcacca ttatcaagga cagctccctc ttatcagcta tcggtgttat ggagttgtgg
     3541 aacggggcta caacagtttc tacaacaacc tatctacctt taacaccact tttatttgca
     3601 gctttttatt acttgattat gacttctatt ttgacagtag ccttgaaagc ttttgaaaaa
     3661 cgtatgggac aaggagataa gaaataatga cagaaacctt gataaaaatt gaaaatttac
     3721 ataaatcatt tggaaagaac gaagtattga agggcatcaa cctcgagatt aaaagaggag
     3781 aagttgtcgt tatcatcggt ccttcaggga gcgggaaatc taccttgctt cgctctatga
     3841 atttgttgga agaagcgacc aagggtaagg ttatctttga gggagtcgat attacggaca
     3901 agaagaatga cctctttgcc atgcgtgaga agatgggcat ggttttccaa caatttaacc
     3961 tctttcctaa tatgactgtg atggaaaata tcaccttgtc acctatcaag accaaaggtg
     4021 agagcaaggc tgttgctgag aagagagccc aagagctttt ggaaaaagtt ggtttgccag
     4081 ataaggcaga tgcttatcca cagagtttgt caggtggtca gcaacaacgg attgccattg
     4141 cgcgtgggtt ggccatggaa cctgatgttt tgctctttga cgagccaact tcagcactag
     4201 atcctgagat ggttggagaa gttctggctg ttatgcaaga tctagccaag tcaggaatga
     4261 ccatggttat cgtaacacat gagatgggat ttgcccgtga ggtggcagat cgtgtcatct
     4321 ttatggcaga cggtgtggtt gttgaagacg gaacaccaga acagattttt gaacaaaccc
     4381 aagaacaacg gactaaagac ttcttgagta aggttttata atctatttta aaatttcaag
     4441 acaagattag tgaaaagctc aaccagagct ttttcttata gtttgaaact ataggattgc
     4501 ctaggaaaga agtgttagag ggtgctggca gtttttggta taatggaaga tatttgaaag
     4561 aaaagagaag tgatatgaca cagattattg atggaaaagc tttagcggcc aaattgcagg
     4621 ggcagttggc tgaaaagact gaaaaattaa aggaagaaac aggtctagtg cctggtttgg
     4681 tagtgatttt ggttggggac aatccagcca gccaagtcta cgttcgcaac aaggagaggt
     4741 cagctcttgc agctggtttc cgtagcgaag tagtgcgagt tccagagacc attactcaag
     4801 aggaattgtt agacctgatt gctaaatata atcaggatcc agcttggcat gggattttgg
     4861 tccagttgcc attaccaaaa catatcgatg aagaggcggt tttattagcc attgacccag
     4921 aaaaggatgt ggatggtttc catcccctaa acatgggacg tctttggtct ggacatccag
     4981 tcatgattcc ctcgacacct gcaggaatta tggaaatgtt ccatgaatat gggattgact
     5041 tggaaggtaa aaatgcagtc gtcatcggtc gatccaatat tgtcggaaaa cctatggccc
     5101 agcttctttt ggcaaagaat gcaacagtaa ccttgactca ctcacgtact cataatcttt
     5161 ccaaggtggc tgcaaaagca gatattctgg ttgttgcaat cggccgcgcc aagtttgtga
     5221 ctgctgactt tgtcaaacca ggtgcggtag tcattgacgt tgggatgaac cgcgatgaaa
     5281 atggtaagct ctgtggggat gttgattatg aggcggttgc cccacttgct agccacatta
     5341 cgccagtccc tggaggtgtc ggtcctatga ccattactat gctgatggag caaacctatc
     5401 aggcagcact taggacattg gatagaaaat aagagaaaca ttcgattaat agagtgtatt
     5461 ttctatagct atatctaaca atggcagaaa tgaatattaa attttagata taagattaca
     5521 aaaggaggtc tgcgtctcct ttttattgta taataaaagt gagaggaaaa aaggatgaaa
     5581 gtgattaatc aaaccttatt agaaaaagtt attattgaac gttctcgttc aagtcataaa
     5641 ggagattatg gtcgtctgct gctgcttggt ggtacttatc cttatggagg tgccatcatc
     5701 atggctgctt tagcagctgt aaaaagcggt gcaggattgg tgaccgttgg aacggacagg
     5761 gaaaatatcc cggatctgca cagccattta cctgagccta tggccttttc tcttcaagac
     5821 cagcaattgt taaaagagca attggagaag gcagaagtta tcttgctggg gcctggttta
     5881 ggagacaatg cttttggaga agatttagtt aaacaggtct ttgctggctt aaaacagaat
     5941 cagattttga ttgtagacgg aggtgccttg accattcttg ctaggacaag tttgtcattt
     6001 ccctcaagcc agcttatcct aactcctcac caaaaagaat gggaaaaatt atctggaatt
     6061 acgattgaaa agcaaaagga agatgcaacg gctagtgttc tgacttcctt ccctcaagga
     6121 acaattttgg tggagaaagg cccagctact cgtatttggg aagttggcca atctgattat
     6181 taccagttac aggttggtgg tccttatcag gcgactgggg gaatgggaga tactctggct
     6241 gggatgatcg caggatttgt aggccaattc cgacaggcca gtctctacga gcgtgtggca
     6301 gtagcgaccc atcttcattc agccatagcc caagaactat ctcaagaaaa ttatgtggtc
     6361 ttaccgacgg aaatcagcag gtatctttcc aaaataatga aaattatttg tcaacaagat
     6421 agggtaagca aagataaact tgtttaaaat agacagataa atctacttcc ttctcttaat
     6481 ttatgattct tgaaatgcgc ctgctttaag gctaaactag acgtatcaaa gttaagagtt
     6541 tgtttttgta actggaaaga gatgaacaaa tcaaatacat aaaagataaa ctcattttta
     6601 ttcgtttggt aaaaataaaa tgcatatttt taaagaaaga ttgatggaaa atgtagattt
     6661 gatgttttta cttgaataaa aagcggtttc atagtaaaat aagaactgag aatgatggag
     6721 gtaaatcggt ggaaaatctg aagaaaatgg caggtatcaa ggctgctgag ttcgtgagcg
     6781 atggaatggt cgttggactt ggaacaggct cgactgccta ttattttgtc gaagaaatcg
     6841 gtcgtcgtat caaggaagaa ggattgcaga ttacagctgt aacaacttct agtgtgacca
     6901 ctaaacaggc agaaggcctt aatatcccgc tcaagtctat tgaccaagta gactttgtcg
     6961 atgtgacggt tgatggcgcg gatgaagtgg atagtcagtt taatggaatc aaaggcggtg
     7021 gtggtgccct tctcatggaa aaggtggtcg caacaccatc aaaagaatac atttgggtgg
     7081 tggatgaaag caagctggtc gaaaaactag gtgcttttaa attgccagta gaagtggttc
     7141 agtatggtgc agagcaggtc tttcgtcatt ttgaacgagc tggctacaaa ccaagtttcc
     7201 gtgaaaaaga cggccaacgt tttgtgacgg atatgcagaa ttttatcatt gacctcgcct
     7261 tggatgtcat tgaaaatcca attgcttttg gacaagaatt ggaccatgtc gttggtgttg
     7321 tggagcatgg tttattcaac caaatggtgg ataaggtaat cgttgctgga cgagatggag
     7381 ttcagatttc aacttcaaaa aaaggaaaat agaagggggc ataagatgtc taaatttaat
     7441 cgtattcatt tggtggtact ggattctgta ggaatcggtg cagcaccaga tgctaataac
     7501 tttgtcaatg caggggttcc agatggagct tctgacacac tgggacacat ttcaaaaaca
     7561 gttggtttga atgtcccaaa catggctaaa ataggtcttg gaaatattcc tcgtgaaact
     7621 cctcttaaga ctgtagcagc tgaaagcaat ccaactggat atgcaacaaa attagaggaa
     7681 gtatctcttg gtaaggatac tatgactgga cactgggaaa tcatgggact caacattact
     7741 gagcctttcg atactttctg gaacggattc ccagaagaaa tcctgacaaa aatcgaagaa
     7801 ttctcaggac gcaaggttat tcgtgaagcc aacaaacctt attcaggaac ggctgttatc
     7861 gatgattttg gaccacgtca gatggaaact ggagagttga ttatctatac ttcagctgac
     7921 cctgttttgc agattgctgc ccacgaagac attattcctt tggatgaatt gtaccgtatc
     7981 tgtgaatacg ctcgttcgat tacccttgag cgtcctgccc ttcttggtcg catcattgct
     8041 cgcccttatg taggtgaacc aggtaacttc actcgtacgg caaaccgtcg tgacttggct
     8101 gtatctccat tttccccaac tgttttggat aaattgaatg aggctggtat cgatacttat
     8161 gctgtgggta aaatcaacga tatctttaac ggtgctggta tcaaccatga catgggtcac
     8221 aacaagtcaa atagtcatgg aattgataca ctattgaaga ctatgggact tgctgagttt
     8281 gaaaaaggat tctcattcac aaacctagtt gactttgatg ccctttacgg ccatcgtcgt
     8341 aatgctcacg gttaccgtga ttgcttgcat gagtttgatg aacgcttacc tgaaattatc
     8401 gcagctatga gagagaatga ccttctcttg attactgcgg accatggaaa tgacccaacg
     8461 tatgcaggaa cggatcacac tcgggaatat attccattgt tggcctatag ccctgccttt
     8521 aaaggaaatg gtctcattcc agtaggacat tttgcagata tttcagcgac tgttgccgat
     8581 aactttggtg tggaaactgc tatgattggg gaaagtttct tagataaatt ggtataagat
     8641 gacgcgctat gctttgctgg tgagaggtat caatgttggt ggtaagaata aggtcgtcat
     8701 ggcggagctt cgtcaagaat tgacaaactt gggactggaa aaggttgaga gctacatcaa
     8761 cagtggcaat attttcttta cttcgataga ttccaaagcc caattggttg aaaagctaga
     8821 gactttcttt gcagtctatt atccatttat tcagagcttt tctttactga gtctagagga
     8881 ctttgaggcg gaacttgaaa atctaccagc ttggtggagc agagacttgg cacgaaaaga
     8941 ttttctcttt tacactgagg gtttggatgt ggaccaagtc atcgcgacag ttgaaagttt
     9001 agagctgaaa gatgaagtgc tttattttgg aaaacttggg attttctggg ggaaattttc
     9061 tgaagaatcc tattctaaga ctgcctatca taagtacttg ctgaaggtgc ctttctaccg
     9121 ccacattact attcgtaatg ctaaaacctt tgacaaaatt ggtcaaatgc taaaaaaata
     9181 ataaaggaga cacacaatga catttttaaa caaaatccat gaaactgcta ctttcctgaa
     9241 agaaaaggga attgcagccc ctgagttcgg tctaatcctt ggatcaggac ttggagaatt
     9301 ggcagaagaa atcgaaaatc cagttgtagt agactatgct gagattccaa actggggccg
     9361 ttcaacagta gtcggtcatg ctggtaaatt ggtatatggt gaactggcag gtcgcaaggt
     9421 cttggctctt caagggcgtt tccatttcta tgaagggaat cctctggaag tggtgacttt
     9481 cccagttcgt gtgatgaaag ttcttggatg tgaaggtgtt attgtaacca atgcagctgg
     9541 cggtatcgga tttggtcctg gtaccttgat ggctatctca gaccatatca acatgacggg
     9601 gcaaaatcca ttgatgggtg aaaacttgga tgactttggc ccacgtttcc cagatatgtc
     9661 tagggcttac acaccagaat accgtgccac tgcccatgaa gtggctaaaa aacttaatat
     9721 caagcttgat gaaggtgtct atatcggagt tactggtccg acttatgaaa caccagcaga
     9781 aattcgttcc tataagacac tgggagcaga tgcagttggt atgtctacgg ttcctgaagt
     9841 tatcgtggca gcccactctg gcttgaaagt tctgggaatt tcatgtatca ctaactttgc
     9901 ggccggtttc caagaagaac tcaatcacga agaagttgta gaagtgactg aacgtgttaa
     9961 aggtgatttc aaaggcttgc ttaaagcgat tcttgctgaa ttgtaagaaa aaagatttaa
    10021 aagggggagt gcctctgtct tttcaggatt gactgcctat ccgtattaaa gaagaaacag
    10081 aggaatacta tgagcttctt cctgctctta taactgaaag aagcggaaga ataggtatgt
    10141 ctgatctgat agccagcatt gtgaaggaca agattctagg atactagcat tagcttccta
    10201 gcattagctt cctagccaag cagactagta tgataaggag agatgagaat gaattgactt
    10261 tctgaatttc tcagtcttat catatatagc acaatgagat ttcgcttgag tctgcttgta
    10321 aataaacgaa aagaaagata agaaataatg aaaattggtc aacgaattat gcgctttggc
    10381 ataaaaaatt aagtatcgga gttgtatctg ttgtagtcgg ctttgatttc tagctccagc
    10441 tggaatttca gccaatgaag taaagcaaga tgtaacatct gaagtggtaa taggtgtgct
    10501 agattctaag gaggaattga aagagtcaga aaatgatgct ccaaaactag aaactcctct
    10561 tagagaggag ccaagactag ctcctcaaac gcttccggaa gcaagtgaag ttcttgaaaa
    10621 caaaagggaa gagtcaaaag tagagataac agagccagct caagcggatg atatccgcaa
    10681 ggttgttggg gaattagcca aggatataag tattactaag ttgtatatga caggtcattc
    10741 tcttggaggc tacctagctc agattgcagc ggttgaagat taccaaaaat atcctgattt
    10801 ttataaccat gtattgagga aagtgacaac tttcagtgct cctaaagtga ttacttccag
    10861 aactgtttgg gatgctaaga atggtttctg agatgttggt ttggaaagtc gtaaattagc
    10921 tgttagtgga aaaattaagc attatgtggt tgataatgac aatgttgtga ctcccttgat
    10981 tcataataat cgtgatattg ttacatttac aggtaattca cgctttaaac accgttctcg
    11041 tggctatttt gaaagtccaa tgaatgatat tcctaacttt aatattggta aacaagctac
    11101 cttggataaa catggttatc gtgatccgaa attggataaa gtgcgattct ttaagaaaca
    11161 ggctctacct caatcttcta gtcaaccaag cgctgaacca atggaaaata ttgccttagg
    11221 aaaacaggtt actcaaagtt cgacagcttt cggaggagat gctagaagag ctgtggatgg
    11281 caaagtcgat ggtaactatg gtcacaattc tgtcactcat acaaacttcc aatctaagcc
    11341 ttggtggcaa gtagatttgg ctaaagaaga aaccattcgc caaatcaata tttacaaccg
    11401 aacagacact gcccaggata gattggcaaa ctttgatgtc attcttttag acagttctgg
    11461 taaagaaatt gagtgaaaac gtataatatc tcctaaagat gtgtcagcac aaattacgat
    11521 taaccataaa aaagcgcgct atgttcggat tgagctagaa ggctataatg ccctcagtct
    11581 tgcagaagtt gaagttttcc gctttatagc tacgaatgct gaaacggcga cacaagtttc
    11641 taagccagtt caaccaatca gtcagactcc tgtgaaagat aaaacattga caattcaaca
    11701 cagtggagct tacattgccc gctactccat aacttgggaa gaagttccag tagataaaga
    11761 tggaaaccaa gttgttcgta gtcattcttg ggaaggaaac ggttgcaacc agactgcagg
    11821 ttttgtcctc aacctcccaa tcaaagaaaa tatgagaaat ctgcgagtta agattgagaa
    11881 aaagacgggc ctactatgga atagatggca aacaatctat gaaaacagac caattttagc
    11941 tcaaccccac cgtaaaatta cccattgggg tacgacattg aattccaagg tgagtgacga
    12001 tgatgtcttg taatctgatg gtacaatgac agttagtttg tctagtttat aagaaagtac
    12061 tacctgagct tgaatagaac tcaggtagct ctatgaaaga acaaaattaa tactcaatga
    12121 aaatcaaaga gcaaactagg aaactagccg caggttgctc aaagcactgc tttgaggttg
    12181 tagataagac tgacgaagtc agtcacatat ataatccaag gcgacgttga cgtggtttga
    12241 agagattttc gaagagtata aacagaaagg tagagcgcgt gttctaattt gaacacgagt
    12301 agaaaacttt tctaaaaaca aaaacgaaag gatgggtaaa ctgtattcgc tgaactgaat
    12361 acgggcgact ctcctctaaa tcaaaattaa gaaagaaagg aattgacccc accctaaaag
    12421 cagtgggaaa aagatagttg atctagcgag catcgctcac tgcgcccaac tcctattttc
    12481 ccttcgcttt ttgatgggtt tggtatcttt ctcaatataa aatataaaat aaagaaaggt
    12541 agagcgtgtg ttttgatttg aacacgagcg gaaaactcgg aaaatagata atctgactga
    12601 aaaatcagga tttctcgtca ggttcctaat tttcagtcgt tttcttctcg ctctttgtat
    12661 cataaattat gtctatccat attgctgctc agcagggtga aattgctgat aaaattcttc
    12721 ttcctgggga tcctcttcgt gctaagttta ttgcggagaa tttccttgat gatgctgttt
    12781 gttttaacga agtgcgtaac atgtttggtt acactggtac ttacaagggt cactgtgtat
    12841 ctgtcatggg aactgggatg ggaatgccat ctatttcgat ttatgcgcgt gagttaatcg
    12901 tagactacgg tgtgaagaaa ttgattcgtg tgggaactgc aggttctttg aatgaagagg
    12961 ttcatgttcg tgaattagtt ttggcgcagg cggctgcaac caactcaaac atcgttcgta
    13021 atgactggcc acagtacgat tttccacaaa ttgctagctt tgatttgctt gataaagcct
    13081 accatatcgc caaaaaactt ggtatgacta ctcacgttgg gaacgttttg tcatctgatg
    13141 tcttttactc aaattacttt gaaaagaata tcgagcttgg taaatgggga gtcaaggctg
    13201 tggaaatgga agcagcagct ctttactatc ttgctgccca ataccatgtt gatgcgctag
    13261 ctatcatgac catctctgat agcttggtca atccagacga agacacaact gcagaagaac
    13321 gtcaaaatac cttcactgat atgatgaagg ttggtttgga aaccttgatt gcagaataat
    13381 tatagccaaa aaggaagccc tttatgaatt gtaggacttc cttttcttat ccagaaattg
    13441 atctagctct ctctgatttc gaagaatagt gactttatgt gaatattctt ggcaaagttt
    13501 ttggtaattt tctttttgag ttttgctacg cccatcccaa agaatccatc tgataaactc
    13561 ccagtcaaag cgttcagggc aatctaccgc catactttct ctgacttttc cacggtattt
    13621 aagataacgc ttaaaggctc taaagagaca ggtcaatggc gaaaaattga gaaagatgat
    13681 ttggtcagct tcttgcattc gttcttggta gtagcaccaa gaataattac catcgatgac
    13741 ccaagcttta tacttggtga gaaagttttt tatctcggtt aacatccatt cgcagtcact
    13801 gtcttgcaaa ccaggttgaa attggagtgt gtccatgtgc agttttggaa tggagtagta
    13861 gttagataac ttttctgcta tagttgactt accagaacca gaatatccga taattgcgat
    13921 tttcattttc taccttttcc tatttggaga caaaaaaaca gcctctatgg actgtttctt
    13981 atttaacaag tttagctgaa agacgagctt tatcgcggct tgctttgttt ttgtgaatca
    14041 aacctttagt ttctgcttta tcgatagctg agctagcagc acggaaaagt tcttcagatg
    14101 ggtttgcttc gaaagctttt atagcagtac gcatagctga tttttgagct gagttctttt
    14161 cgttttgttt aacgttcaat tcagcgcgtt tgatagctga tttaatgttt gccaatgttc
    14221 ttacctccat atttactaac tataccatta tatatgaaaa cttacgtttt gacaagggaa
    14281 aattactttt ttaagtaaat ttcatcgatt tcatggttct tgcttttatg aagaatcact
    14341 tctgcacgat ttctggttgg ttcaatataa ttttgcagat ttgtgagatt gatactgatc
    14401 cagacctgat gggcaaagga ctccacttcc ccaatcggca tctgagtaaa acgataatag
    14461 tagctatcag ggtcgttttg ggctagactc agcattttca agaaacggtc cagataccaa
    14521 ctttcaatat catccactcc agcatctaca tagatggaaa agtcaaagaa gtcagtgata
    14581 tagagacgat cgttttgtgg attttgaaag acattgattc cctcaacaat tacaaaatca
    14641 gcagctttga cactttgttt ctcttcgggt acgatgtcgt aaacttcatg agaatagaca
    14701 ggaatatcta catcttgtcc atttttgatg cggtccaaga agttgagaag agcttccata
    14761 tcatagcttt caggaaatcc tttacgattt aaaatcccct gctcaatcaa ggtttgattg
    14821 ggatagagaa aaccatcagt tgtaaccaac tcaaccgtag catctgtaaa cgtacgggac
    14881 agtaggattt gaagtaggcg actggttgtg gattttccaa cggcaacact cccagaaacc
    14941 ccaataataa aaggttgaga tttactttca cgttggagga aaattccttt tgaaaaggct
    15001 aaatcttcct tagttcgctt gtaaatctga atcaaatgag ccaaggggag atagatatct
    15061 gtaacgtctt ggagactgat ttggtcatta aaactcttga tagattccaa ttcttcttct
    15121 gtcaaaggag gtgttgtctt tcgatgtaaa gattgccaag tctggcggct gattttttca
    15181 aaatgtaaaa attcgttggt catttgtttt cccctagata ttttgtctat cataccataa
    15241 aaagctaaaa aaataaagcg gtttcttgaa tgtggtaagt caatggtata tagtataagt
    15301 gatcaattta ttgatgaaat aggagataga ttctactgat ttataagctt ataaaggggg
    15361 ctttttagtt cacaacttat aggaggtgta ctatgaaaat cttaaaacgt tacatattgg
    15421 aactctgttt tattttaagt tttgctttac cttttataaa aggaaccaat gcagataatg
    15481 gtagatgctt tgtggaaacc tattacggtt ttactttttt gatggaacat gctattgtaa
    15541 cagctgtctt tatttgttcg ttcttaattg ctttcttact aaaaaacgat ggacgaaatg
    15601 gattgctgcg ggtagttatt gctttttagt cctacggatt gcgacagagg gttatttctt
    15661 ccacatgtca ctagaagatt tgatacgact ttggacaagt ttggaaatcc tgacacaaac
    15721 gtatcagttg ggcttttatc taaacatttt gttaggaatt ttgttgataa taaagtattt
    15781 taaggttaag caatagaaat aataatctgc ctgattatag atgataaact ttttgttttt
    15841 atctggactg tgatatttat agttctaaat ggtttattat ttataagtgt tgaaagagat
    15901 tgaagaaaat ggcttcgtga cttgctttct tcggtctttt tcgttttatt atcaatttgt
    15961 aaacgattta caatttgagt caaattatgg taaaatagtg tcatgagtaa aatgtactat
    16021 gcagaaaatc ctgacgctgc tcacgacatt catgagttga gagtggactt gttgggagaa
    16081 aaaatgactt ttttgacaga tgcgggtgtt tttagcaaga aaatggttga ctttggaagt
    16141 caactcttgc tcaagtgtct ggatgtcaac caaggagaga cagtcctaga tgtaggctgt
    16201 ggttatggtc cattaggttt gtccttggtc aaggtttacg gagttcaggc gaccatggtt
    16261 gatattaaca ctcgtgccct ggatttagct cagagaaatg ctgaaaaaaa taatgcaaaa
    16321 gcgacgatat ttcaatctaa tatttatgca agagttcaag ggcgatttga ccatgtcatt
    16381 tccaatcctc ctatccgtgc gggcaagcaa gtcgttcatg aaatcatcga gaaaagtaaa
    16441 gatttcttgg aaattggtgg agatttgacc atcgtcatcc agaaaaaaca aggagctcca
    16501 agtgccaagt ctaagatgga agatgtgttt ggcaattgtg aaatccttaa aaaagacaag
    16561 ggatactata tccttagaag tgtgaaagaa tgagagcagt tgatttaatc caaaaaaaac
    16621 gagacggtca agaactgact tcaagtgaaa ttgaatggct gatagaaggc tatgtatcag
    16681 gaactgttcc tgattatcag atgtctgcct ttgctatggc tgtttatttt aaaggaatga
    16741 cgactcgaga aatctctgat ttaacgatga atatggttaa gactgggcaa gagtttgacc
    16801 tgtcagccat tgatggtgtg aaagttgaca agcattctac tggtggtgtc ggcgataagg
    16861 taaccttgat tttagctcct cttgttgcta gcttcggtgt gcctgtagca aaaatgagtg
    16921 gtcgtggtct tggccataca ggcggaacaa ttgataaatt ggagtccatt aagggctatc
    16981 aagtagaacg tagtcaagag gattttattc gtcaggttca ggatattgga gtatctgtca
    17041 ttgggcaatc agaccagctg gtcaaagcag ataagcttct ctatgccctt cgtgatgtga
    17101 ccgcaactgt cgacacgatt cctttgattg cgagttcggt gatgagcaag aaaattgcgg
    17161 caggggcgga tgctattttg ctagacgtga ctgtcggtga gggtgccttc atgaagacgg
    17221 ttgatgaggc gcgtgagttg gctcaaacta tggtagatct tggtaaggtt gttggacgga
    17281 agacagtagc agtgattacg gatatgagcc agcctttggg acgagcgatt ggaaatcgtc
    17341 tggaaatcct tgaagcattg gagattttgc aaggtcaagg acgtcaggat attacccact
    17401 ttatttgtga attggctcaa attatgcttg gtttggcaaa tgttaacaaa actgttgaag
    17461 aagttcgcca acatcttgaa aatggtcaag cactggctaa gtttgaggaa atggtgcaag
    17521 cccaaggtgg agacttggaa gacctctatc gtcctgtaaa tgtagctcat gtggtggaaa
    17581 tccctgctca ggaaacgggt gtcatttcag ctcttccagc tatggacttt ggtctttatg
    17641 ccatgaggct gggtgctggt cgtgcagtta agtctgatgc cttggactat gaaacaggaa
    17701 ttgtttttga aaagaagatt ggagactccg ttcaaaaggg tgaaattgtt gcaaaagtat
    17761 acacaaatga aaaaattcca cctcaactag ttacagattt ccaaaaatgt gttaaaataa
    17821 gtgatgaagt gaaaaaaatc cgtgaaattg tagaaatcat ctcctaaatg gagggaaaac
    17881 cgaaatgaaa ttaaataaat atatagatca tacgctttta aaacaagatg caaagaaaaa
    17941 acaaattgat agtttgttgt ctgaggctag agagtatgac tttgccagtg tttgcgttaa
    18001 tccgacctgg gttgaacatg ctaaaaaagg acttgaaggc acagatgtta aggtttgcac
    18061 agtagtaggt ttccctttgg gagcaacaac ttcagccgtg aaagcatttg agacaaaaga
    18121 agctatccaa aatggtgcag atgagattga tatggtgatc aatgttggag atctcaaatc
    18181 aggtaattta gccttggttg agtcagatat tcgcgcagta gtggaagcaa gtggtgataa
    18241 gttagtgaaa gtcattattg aagcttgcct tctgacagac caagaaaaaa ttgttgtttg
    18301 ccaattggcc caaaaagctg gggctgactt tgtcaaaaca tctactggct tttcaactgg
    18361 tggtgctacg atagcagatg ttacattaat gcgtgaaaca gttggatctg atatgggagt
    18421 caaggctgct ggtggagctc gttcttatgc agatgctctt gcctttgtcg aagcaggtgc
    18481 gacccgtatc ggaacgtcag ctggggtagc tattttaaaa ggagaattgg cagatggcga
    18541 ctactgagtt gattgaactg gcaattgaaa ccagcaaaca tgcctatgtc ccctattctc
    18601 actttcctat cggagcagtt ttagtagcca aagacggaaa tgtttacacg ggagtgaata
    18661 tcgaaaatgt tagctatcct ttgaccaatt gcggtgagag aacagctatt tttaaggcta
    18721 tatctgaagg ccaaagagaa ttttcagaat tgattgtcta tggtcaaact gaaaaaccga
    18781 tttcaccatg tggtgcttgt cgccaagtaa tggtcgaatt ttttgaacaa gatttaaaag
    18841 tgaccttagt cgcaaaagat aaatcgacgg tcgagatgac ggtcggggag ttacttccat
    18901 attcttttac agacttaaat tagtctgagt cgctctttga gtggcaaggg tctttgtgac
    18961 caatcaatcc atacttgcaa catcgttgca catcttattt aggaggttca gtaatgaaca
    19021 agaaacaatg gctaggtctt ggcctagttg cagtggcagc agttggactt gctgcatgtg
    19081 gtaaccgctc ttctcgtaac gcagcttcat cttctgatgt gaagacaaaa gcagcaatcg
    19141 tcactgatac tggtggtgtt gatgacaaat cattcaacca atcagcttgg gaaggtttgc
    19201 aggcttgggg taaagaacac aatctttcaa aagataacgg tttcacttac ttccaatcaa
    19261 caagtgaagc tgactacgct aacaacttgc aacaagcggc tggaagttac aacctaatct
    19321 ttggtgttgg ttttgccctt cataatgcag ttgaagaagc agctaaagaa cacactgact
    19381 tgaactatgt cttgattgat gatgtgatta aagaccaaaa gaatgttgcg agcgtaactt
    19441 tcgctgataa tgagtcaggt taccttgcag gtgtggctgc agcaaaaaca actaagacaa
    19501 aacaagttgg ttttgtaggt ggtatcgaat ctgaagttat ctctcgtttt gaagcaggat
    19561 tcaaggctgg tgttgcgtca gtagacccat ctatcaaagt ccaagttgac tacgctggtt
    19621 catttggtga tgcagctaaa ggtaaaacaa ttgcagccgc acaatacgca gccggtgcag
    19681 atattgttta ccaagtagct ggtggtacag gtgcaggtgt ctttgcagag gcaaaatctc
    19741 tcaacgaaag ccgtcctgaa aatgaaaaag tttgggttat cggtgttgac cgtgaccaag
    19801 aagcagaagg taaatacact tctaaagatg gcaaagaatc aaactttgtt cttgtatcta
    19861 ctttgaaaca agttggtaca actgtaaaag atattgctaa caaggcagaa aaaggagaat
    19921 tccctggcgg tcaagtaatc gtttactcat tgaaggataa aggggttgac ttggcagtaa
    19981 caaacctttc agaagaaggt aaaaaagctg tcgaagatgc aaaagctaaa atccttgatg
    20041 gaagcgtaaa agttcctgaa aaataatgga tggaagtcat ttcttaggaa gcggccaagg
    20101 gccgcttttt taagagttga gacaaagtca ttttgtctca gtaaagcttg ctactagaca
    20161 aactagcaag ttttattgaa ataaaataag actctgaaag gaagagcaca tggcacacga
    20221 aaatgtcatt gagatgcgtg atattaccaa ggtgtttggt ggatttgttg ccaacgacaa
    20281 aatcaacttg cacctacgaa aaggtgaaat tcatgcactt ttaggagaaa atggggctgg
    20341 taagtccacg ctaatgaaca tgttagcagg ccttcttgaa ccaactagtg gtgaaatcgc
    20401 ggtcaacggt caagttgtca atctcgactc cccatctaaa gcagctagct tgggaatcgg
    20461 gatggttcac cagcacttta tgttggttga agccttcaca gtggctgaaa acatcatttt
    20521 aggtagtgaa ttgactaaaa atggtgtgct agatatcgct ggagctagca aagaaatcaa
    20581 ggctctttct gaacgttatg gcttagctgt tgacccttct gccaaggtag cagatatctc
    20641 agttggagcc caacaacgtg tagaaatttt aaaaacactt tatcgggggg ctgatatcct
    20701 tatctttgac gaaccaacgg ctgttttgac tccatcagaa attgatgagt tgatggctat
    20761 tatgaaaaat cttgtcaaag aaggaaaatc aattatcttg attacccaca aattggatga
    20821 aattcgcgca gtttctgacc gtgttacagt tatccgtcgt gggaaatcaa ttgaaaccgt
    20881 tgaaattgca ggggctacca atgctgattt ggcggaaatg atggtaggac gttctgtttc
    20941 ctttaaaaca gagaagcaag cctctaaacc aaaagaagtg gttttgtcta tcaaagattt
    21001 ggtggtcaat gaaaaccgtg gtgttccagc tgttaaaaat ctgtccttgg atgttcgtgc
    21061 tggagagatt gttggtattg cggggattga tggaaatggt cagtctgaac tgattcaagc
    21121 cattacaggt cttcgtaagg ttgaatctgg tagcattgag ctaaaaggag attcaattgt
    21181 aggcttgcac ccacgtcaga ttacagaact aagtgttggg cacgttccag aagaccgtca
    21241 ccgtgatggc ttgattttgg aaatgatgat atctgaaaat attgcccttc aaacctacta
    21301 taaagaacca catagtaaaa atggaatttt gaattattca aatattactt cttatgctaa
    21361 aaagctgatg gaagagtttg atgttcgcgc tgccagtgaa ttagttcctg cagctgcact
    21421 ctcaggagga aatcaacaaa aagcaattat tgctcgtgaa attgatcgag atcctgatct
    21481 ccttatcgtt agccagccaa ctcgtgggtt ggatgtcggt gccattgagt atatccacaa
    21541 acgcttgatt gaagagcgtg ataatggcaa ggctgtcctt gttgtcagct ttgaattgga
    21601 tgagatttta aacgtctcag accgtattgc cgttatccac gatggtaaga ttcaaggtat
    21661 tgtatcacca gaaacaacca ataaacaaga acttggtgtc ttgatggctg gtggaaactt
    21721 gggaaaggag aagagtgatg tctaaaaaat tacaacaaat ttcggttccc ttgatttctg
    21781 tatttctagg aattttactc ggagccattg tcatgtggat cttcggttat gatgctattt
    21841 ggggctacga agaattgttc tatacagcct ttggcagtct gcgtgggatt ggagaaatct
    21901 tccgtgctat gggtcctctg gtcttgattg gtcttggttt tgcggttgcc agtcgagctg
    21961 gtttctttaa cgtcggactt cctggtcagg ctttggcagg ttggattctc agtggttggt
    22021 ttgcgctgtc gcatccagat atgccccgtc ccttgatgat tctagcaacc atcgtgattg
    22081 ccttgattgc tggtgggatt gtcggagcga ttccaggtat tcttagggcc tatctaggga
    22141 cgtcagaggt tattgtaacc atcatgatga actacattgt cttgtatgta gggaatgcct
    22201 ttatccatgc tttccctaaa gacttcatgc aaagtacaga ttcgaccatt cgtgttgggg
    22261 ctaatgcaac ctatcagaca ccttggttgg ctgagttgac tggtaactca cggatgaata
    22321 ttggtatttt ctttgccatc attgccgttg cagttatttg gttcatgctc aagaaaacaa
    22381 ctcttggttt tgaaatccgt gcagttggtc ttaatccaca tgcttcagaa tatgctggta
    22441 tttctgccaa gcggactatt atcctatcta tgattatttc aggtgccttg gcaggtcttg
    22501 gtggagctgt tgaaggtttg ggaaccttcc agaacgtcta tgttcaaggt tcgtcattag
    22561 ctatcggatt taacggaatg gcggttagtt tgcttgcggc caactcacca attggtatac
    22621 tctttgcagc cttcctattt ggcgttctcc aagttggggc tcctggtatg aatgcggcgc
    22681 aggtaccatc tgagcttgtc agcattgtaa cagcgtctat tatcttcttt gtcagtgttc
    22741 attaccttat cgaacgcttt gtcaaaccga aaaaacaagt taaaggaggt aagtaaagat
    22801 gtctattata accttgctcc ccctcttggt gtcttctatg ctgatttact cagcacctct
    22861 catctttaca agtatcggtg gtgttttctc tgaacgtggt ggtgtggtaa acgttggcct
    22921 tgaaggaatt atggttatgg gtgccttttc tggagttgtc tttaaccttg aatttgcaga
    22981 acaatttgga gcagcaactc catggctatc cttgcttgta gcaggattgg ttggtagtgt
    23041 tttctctatc atccacgcag cagcgacggt tcatttccgt gcagaccatg ttgtcagcgg
    23101 tacggtattg aacttgatgg cgcctgcctt ggctgttttc ttagttaaag ttctttataa
    23161 caaaggacaa accgacaacc taagtcaaac ttttggacgc tttgatttcc cagtcttggc
    23221 aaatatccca gtgattggtg atatcttctt caagtcaact agtctacttg gttatctggc
    23281 gattgccttc tcattccttg cttggtttat tctctttaag actcaatttg gtcttcgtct
    23341 ccgctctgtc ggtgaacatc ctcaagcagc ggataccttg ggaatcaacg tctacaagat
    23401 gagatattta gggattatta tttcaggttt tctaggtgga attggcggag cgatttatgc
    23461 gcaatcaatc tcagttaact tctcagtgac aactattgtt ggacctggat ttatcgccct
    23521 tgctgcgatg atctttggga aatggaatcc aatcggtgct atgctttcta gtctcttctt
    23581 tggactttca caaagtttgg ctgttatcgg ttctcaattg ccgttcctac aaggagtgcc
    23641 agcggtttat cttcaaattg caccttatgt tttgacaatt cttgtcttag cagccttctt
    23701 tggaaaagca gtcgcaccaa aagcagatgg tatcaactac atcaaatcaa aataagcata
    23761 caaaaaaacg tcagtccaat ga
//
LOCUS       DBERDK010000026        23081 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.026, whole genome shotgun sequence.
ACCESSION   DBERDK010000026 DBERDK010000000
VERSION     DBERDK010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 23081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23081
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.026"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(103..453)
                     /gene="rbfA"
                     /locus_tag="ACL73L_001044"
     CDS             complement(103..453)
                     /gene="rbfA"
                     /locus_tag="ACL73L_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HGS0772040.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMLGD
                     LSVAKVYYTILSNLASDNQKAQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
     gene            complement(704..3496)
                     /gene="infB"
                     /locus_tag="ACL73L_001045"
     CDS             complement(704..3496)
                     /gene="infB"
                     /locus_tag="ACL73L_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HGS0772041.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKPAAAPKVEAKPAAPKVSAEKKTEKSEPAKPAVAKEEAKPAEPVAPKTEKVA
                     AKPQSRNFKAEREARAKEQAERRKQNKGNNRDQQQNGNRQKNDGRNGGKQGQSNRDNR
                     RFNDQAKKQQGQQKRRNERRQQEDKRSNQAAPRIDFKARAAALKAEQNAEYARSSEER
                     FKQYQAAKEALAQANKRKEPEEIFEEAAKLAEQAQQVQAVVEVVPEKKEPAVDTRRKK
                     QARPDKNRDDYDHEEDGPRKQQKNRSSQNQVRNQKNSNWNNNKKNKKGNNKNNRNQTP
                     KPVTERKFHELPTEFEYTDGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGET
                     IELLMVDYGIEAKQKVEVDNADIERFFVEDGYLNEDELVERPPVVTIMGHVDHGKTTL
                     LDTLRNSRVATGEAGGITQHIGAYQIVENGKKITFLDTPGHAAFTSMRARGASVTDIT
                     ILVVAADDGVMPQTIEAINHSKAANVPIIVAINKIDKPGANPERVIGELAEHGVMSTA
                     WGGDSEFVEISAKFNQNIEELLETVLLVAEIQELKADPTVRAIGTVIEARLDKGKGAV
                     ATLLVQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGD
                     HFAVYEDEKSARAAGEERAKRALMKQRQATQRVSLENLFDTLKAGELKSVNVIIKADV
                     QGSVEALSASLQKIDVEGVKVTIVHSAVGAINESDVTLAEASNAFIVGFNVRPTPQAR
                     QQAEADDVEIRLHSIIYKVIEEMEEAMKGMLDPEFEEKVIGEAVIRETFKVSKVGTIG
                     GFMVINGKVARDSKVRVIRDGVVIYDGELASLKHYKDDVKEVTNGREGGLMIDGYNDI
                     KMDDVIEAYVMEEIKR"
     gene            complement(3513..3812)
                     /locus_tag="ACL73L_001046"
     CDS             complement(3513..3812)
                     /locus_tag="ACL73L_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HGS0772042.1"
                     /translation="MNKQKISNLLGLAQRAGRIISGEELVVKAIQDGKAKLVFLAHDA
                     GPNLAKKIQDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            complement(3805..4098)
                     /locus_tag="ACL73L_001047"
     CDS             complement(3805..4098)
                     /locus_tag="ACL73L_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HGS0772043.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            complement(4120..5256)
                     /gene="nusA"
                     /locus_tag="ACL73L_001048"
     CDS             complement(4120..5256)
                     /gene="nusA"
                     /locus_tag="ACL73L_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HGS0772044.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            complement(5300..5779)
                     /gene="rimP"
                     /locus_tag="ACL73L_001049"
     CDS             complement(5300..5779)
                     /gene="rimP"
                     /locus_tag="ACL73L_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HGS0772045.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEIISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDELTMEYMDKTRKKTVRIPYSLVSKARLAVKL"
     gene            complement(5905..6540)
                     /gene="trmB"
                     /locus_tag="ACL73L_001050"
     CDS             complement(5905..6540)
                     /gene="trmB"
                     /locus_tag="ACL73L_001050"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HGS0772046.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVE
                     VGSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEVGVPNIKLLWVDGSDLTD
                     YFEDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            complement(6537..7331)
                     /gene="ccrZ"
                     /locus_tag="ACL73L_001051"
     CDS             complement(6537..7331)
                     /gene="ccrZ"
                     /locus_tag="ACL73L_001051"
                     /EC_number="2.7.1.15"
                     /EC_number="2.7.1.229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0051726 - regulation of cell cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell cycle regulator CcrZ"
                     /protein_id="HGS0772047.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            complement(7492..8103)
                     /locus_tag="ACL73L_001052"
     CDS             complement(7492..8103)
                     /locus_tag="ACL73L_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HGS0772048.1"
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGGFFQNESALILVSL
                     LVTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHL
                     AEVSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSN
                     VPSFIFYLLGGLLLGFAYKKSQNLWVSTLVHMLYNSWPLLYYL"
     gene            complement(8254..8487)
                     /gene="blpZ"
                     /locus_tag="ACL73L_001053"
     CDS             complement(8254..8487)
                     /gene="blpZ"
                     /locus_tag="ACL73L_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein BlpZ"
                     /protein_id="HGS0772049.1"
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFIGAGYVVGFWLLILNENQRAN"
     gene            complement(8529..9218)
                     /locus_tag="ACL73L_001054"
     CDS             complement(8529..9218)
                     /locus_tag="ACL73L_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HGS0772050.1"
                     /translation="MKKYQLLFKISAVFSYLFFVFGLSQMTLIIQNYWQFSSQIGNFV
                     WIQNFLSLLFSGVMIWILVKTGHGYLFHIPRKKWLWYSILTVLVVVLQISFNVQTAKH
                     VQSTAEGWAVLIGYSGTNFAELGIYIALFFLVPLMEELIYRGLLQHAFFKHSRFGLDL
                     LLPSILFALPHFSSLPSLLDIFVFATVGIIFAGLTRYTKSIYPSYAVHVINNIVATFP
                     FLLTFLHRVLG"
     gene            complement(9270..9653)
                     /locus_tag="ACL73L_001055"
     CDS             complement(9270..9653)
                     /locus_tag="ACL73L_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HGS0772051.1"
                     /translation="MKYRLFFVIFLSSVLDILLGTFLQISIVSIGWLVLYSGLFEAGV
                     FLLANKGVAVKIKEVDIRNRFKFIFGKTLWFQILLLIFLIIKLYLGLDARLILFYGHI
                     FIVFNALMYLLSSSQVSLKKNKLSS"
     gene            complement(10282..10601)
                     /locus_tag="ACL73L_001056"
                     /pseudo
     CDS             complement(10282..10601)
                     /locus_tag="ACL73L_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826248.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
     gene            complement(11121..11240)
                     /locus_tag="ACL73L_001057"
     CDS             complement(11121..11240)
                     /locus_tag="ACL73L_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000346296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PncF family bacteriocin immunity protein"
                     /protein_id="HGS0772052.1"
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKIFSKSSKKDTDSTL"
     gene            complement(11344..11493)
                     /gene="blpO"
                     /locus_tag="ACL73L_001058"
     CDS             complement(11344..11493)
                     /gene="blpO"
                     /locus_tag="ACL73L_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpO"
                     /protein_id="HGS0772053.1"
                     /translation="MNTKMMSQFSVMDNEMLACVEGGDIDWGRKISCAAGVAYGAIDG
                     CATTV"
     gene            complement(11737..11985)
                     /gene="blpK"
                     /locus_tag="ACL73L_001059"
     CDS             complement(11737..11985)
                     /gene="blpK"
                     /locus_tag="ACL73L_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpK"
                     /protein_id="HGS0772054.1"
                     /translation="MDTKMMSQFSVMDTEMLACVEGGGCNWGDFAKAGVGGAAVVAAL
                     GCAAGGVKYGKILGPWGAAIGGIGGAVVCGYLAYTATS"
     gene            complement(12054..12323)
                     /gene="blpJ"
                     /locus_tag="ACL73L_001060"
     CDS             complement(12054..12323)
                     /gene="blpJ"
                     /locus_tag="ACL73L_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpJ"
                     /protein_id="HGS0772055.1"
                     /translation="MNTKMLSQLEVMDTEMLAKVEGGYSSTDCQNALITGVTTGIITG
                     GTGAGLATLGVAGLAGAFVGAHIGAIGGGLTCLGGMVGDKLGLSW"
     gene            complement(12790..12987)
                     /gene="blpI"
                     /locus_tag="ACL73L_001061"
     CDS             complement(12790..12987)
                     /gene="blpI"
                     /locus_tag="ACL73L_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpI"
                     /protein_id="HGS0772056.1"
                     /translation="MNTKMMEQFEIMDNEELEIVSGGRGNLGSAIGGCIGAVLLAAAT
                     GPITGGAATLICVGSGIMSSL"
     gene            13268..13855
                     /locus_tag="ACL73L_001062"
     CDS             13268..13855
                     /locus_tag="ACL73L_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HGS0772057.1"
                     /translation="MTSYKRTFVPQIDARDCSVAALASIAKFYGSDFSLAHLRELAKT
                     NKEGTTALGIVKAADEMGFETRPVQADKTLFDMSDVPYPFIVHVNKEGKLQHYYVVYQ
                     TKKDYLIIGDPDPSVKITKMSKERFFYEWTGVAIFLAPKPSYQPHKDKKNGLLSKLPS
                     SDFQTKISHCLHCSLKLISHYYQYRWFLLSPRNLR"
     gene            13782..15425
                     /locus_tag="ACL73L_001063"
     CDS             13782..15425
                     /locus_tag="ACL73L_001063"
                     /inference="COORDINATES: protein motif:HMM:TIGR01193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HGS0772058.1"
                     /translation="MVLSSLLVTIINIGGSYYLQGILDEYIPNQMKSTLGIISVGLVI
                     TYILQQVMSFSRDYLLTVLSQRLSIDVILSYIRHIFELPMSFFATRRTGEIISRFTDA
                     NSIIDALASTILSLFLDVSILILVGGVLLAQNPNLFLLSLISIPIYMFIIFSFMKPFE
                     KMNHDVMQSNSMVSSAIIEDINGIETIKSLTSEENRYQNIDSEFVDYLEKSFKLSKYS
                     ILQTSLKQGTKLVLNILILWFGAQLVMSSKISIGQLITFNTLLSYFTTPMENIINLQT
                     KLQSAKVANNRLNEVYLVESEFQVQENPVHSHFLMGDIEFDDLSYKYGFGRDTLTDIN
                     LTIKQGDKVSLVGVSGSGKTTLAKMIVNFFEPYKGHISINHQDIKNIDKKVLRRHINY
                     LPQQAYIFNGSILENLTLGGNHMISQEDILRACELAEIRQDIERMPMGYQTQLSDGAG
                     LSGGQKQRIALARALLTKSPVLILDEATSGLDVLTEKKVIDNLMSLTDKTILFVAHRL
                     SIAERTNRVIVLDQGKIIEVGSHQELMQAQGFYHHLFNK"
     gene            15436..16797
                     /locus_tag="ACL73L_001064"
     CDS             15436..16797
                     /locus_tag="ACL73L_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HGS0772059.1"
                     /translation="MNPNLFRSVEFYQRRYHNYATVLIIPLSLLFTFILIFSLVATKE
                     ITVTSQGEIAPTSVIASIQSTSDNPILANHLVANQVVEKGDLLIKYSETMEESQKTAL
                     ATQLQRLEKQKEGLGILKQSLEKATDLFSGEDEFGYHNTFMNFTKQSHDIELGITKTN
                     TEVSNQANLSNSSSSAIEQEITKVQQQIGEYQELRDAIINNRARLPTGNPHQSILNRY
                     LVASQGQTQGTAEEPFLSQINQSIAGLESSIASLKIQQAGIGSVATYDNSLATKIEVL
                     RTQFLQTASQQQLTVENQLTELKVQLDQATQRLENNTLTSPSKGIVHLNSEFEGKNRI
                     PTGTEIAQIFPVITDTREVLITYYVSSDYLPLLDKGQTVRLKLEKIGNHGTTIIGQLQ
                     TIDQTPTRTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTTKKTYFDYFKDKILT
                     HSD"
     gene            16854..16982
                     /gene="blpC"
                     /locus_tag="ACL73L_001065"
     CDS             16854..16982
                     /gene="blpC"
                     /locus_tag="ACL73L_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="HGS0772060.1"
                     /translation="MDKKQNLTSFQELTTTELNQITGGGLWEDLLYNINRYAHYIT"
     gene            complement(17053..18393)
                     /locus_tag="ACL73L_001066"
     CDS             complement(17053..18393)
                     /locus_tag="ACL73L_001066"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGS0772061.1"
                     /translation="MNIAWILLYTLVTNGLEIVIFFKVDGIDLTFERIFKAFLLKILL
                     AFVFVMISYIVGNVYLSYFMEPLYGIGLSFLLLRGLPKKLLFFYGLFPMILVNLFYRG
                     VSYFVLPFLGQGQVYDGYSFTGLCIIIFNFFISLAFLKWLDYDFTSLRKEILDKAFQK
                     SLTQINWIMGGYYLVMESLSFFEYEQSIQSKTVRHLILVFYLLFFMGVIKKLDTYLKE
                     KLYERLEQEQALRYRDMERYSRHIEELYKEVRSFRHDYTNLLTSLRLGIEEEDMEQIK
                     EVYGSVLKDSSQKLQNNKYDLGRLVNIRDKALKSLLAGKFLKARDKNIVFNVEVPEEI
                     QVEGMSLLDFLTIVSILCDNAIEASVEASQPHVSIAFLKNGAQETFIIENSIKEEGID
                     ISEIFSFGASSKGEERGVGLYTVMKIVESHPNTSLNTTCQNQVFRQVLTVIHTE"
     gene            complement(18407..19144)
                     /locus_tag="ACL73L_001067"
     CDS             complement(18407..19144)
                     /locus_tag="ACL73L_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGS0772062.1"
                     /translation="MRIFVLEDDFSQQTRIETTIEKLLKAHHIIPSSFEVFGKPDQLL
                     AEVHEKGAHQLFFLDIEIRNEEMKGLEVARKIRDRDPYALIVFVTTHSEFMPLSFRYQ
                     VSALNYIDKALSAEEFESRIETALLYANSQDSKSLAEDCFYFKSKFAQFQYPFKEVYY
                     LETSPRAHRVILYTKTDRLEFTASLEEVFKQEPRLLQCHRSFLINPANVVHLDKKEKL
                     LFFPNGGSCLIARYKVREVSEAINKLH"
     gene            complement(19158..19487)
                     /locus_tag="ACL73L_001068"
     CDS             complement(19158..19487)
                     /locus_tag="ACL73L_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HGS0772063.1"
                     /translation="MKHMIIQTQKTVYKVNVDDIYYIQTHPTKAHTVQIVTEEASFNM
                     LQNLSNLENQCGETLMRCHRNCLVNLDKLKSIDFQERILFLGEEGQYAVKYARRRYRE
                     IRQKWLK"
     gene            19764..20093
                     /locus_tag="ACL73L_001069"
     CDS             19764..20093
                     /locus_tag="ACL73L_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HGS0772064.1"
                     /translation="MTDTDPIKRAHTLITDLNKAYQACKQASADDVRFQEQLNSILGF
                     LAKAETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKTKLIL
                     NENQRAN"
     gene            complement(20261..21310)
                     /locus_tag="ACL73L_001070"
     CDS             complement(20261..21310)
                     /locus_tag="ACL73L_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS0772065.1"
                     /translation="MKDLFLKRKQAFRKECLGYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQHFPENHWPILLFVGITSVLLLLWGGIATYMEAPDKLFLLVGEEEIKLHLKRQTG
                     ISLVFWIFVQTLFLLLFAPLFLAMGYGLPVFLLYVLLLGVGKYFHFCQKASKFFTETG
                     LDWDYLISQESKRKQVLLRFFALFTQVKGISNSVKRRAYLDFILKAVQKVPGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSVLAQVFIEQAWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQEVVRGLTSFVLLVELVVGLITFQEKLALLALLGAGLVL
                     LVLYLPYQVKRQMQD"
     gene            complement(21307..22038)
                     /locus_tag="ACL73L_001071"
     CDS             complement(21307..22038)
                     /locus_tag="ACL73L_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0772066.1"
                     /translation="MLEIKNLTGGYVHVPVLKDVSFTVESGQLVGLIGLNGAGKSTTI
                     NEIIGLLAPYSGSININGLTMQGDATSYRKQIGYIPETPSLYEELTLREHIETVAMAY
                     GIEQKVAFERVEPLLKMFRLDQKLDWFPVHFSKGMKQKVMIICAFVVDPSLFIVDEPF
                     LGLDPLAISDLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRAKGNLLQ
                     LREAFDMPEASLNDIYLALTKEEDL"
     gene            22106..22516
                     /locus_tag="ACL73L_001072"
     CDS             22106..22516
                     /locus_tag="ACL73L_001072"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HGS0772067.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPK
                     EHYRNLLEMDATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA"
     gene            22526..22813
                     /locus_tag="ACL73L_001073"
     CDS             22526..22813
                     /locus_tag="ACL73L_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772068.1"
                     /translation="MKLSNLLLFAGAAAGSYLVTKNRQTITDEVLNTTDRVQAIKDDV
                     DIIQNSLQIINQQKELIKEYQEDLTYKFKVLEKDIQTRLAVIKEMQGTEDK"
BASE COUNT         7251 a         5027 c         3936 g         6867 t
ORIGIN      
        1 agaggcgtac tattctagtt tcaatatatt ttaaaagaat atttttccat ttcaaattca
       61 acctattttc ctccaccaaa aaagaggggc aacccctctt ctttagttct tatccagatt
      121 gcgtagcatc tcgtcaatct tgtttccata ctcgatggac tcgtctttga cgaaggtcaa
      181 atctgggatt ttgtacaatt tcaaattgcg accaagttca cgtttgatgg taccagttgc
      241 tttttcaagc ccgatttggg ctttttggtt atccgaagca aggttactca aaatggtgta
      301 ataaaccttg gcaacagaca agtcacccaa catctgaaca tctgtgatgg tcacaccttg
      361 gacacgtgga tcacgaactt tcttttgcaa aatctcattg acttcacgct tgatttccat
      421 gcccacacga tctgtacgga aatgatttgc catgatattc ctttctctac tttgaaccaa
      481 aagctaggcc agcagaccta gctattttaa acttcttgat tttcagttta aattgaaacg
      541 aagttcaatt tgaagtcatc tgcttctttc gccgtggtga aagtgatgga actgatttat
      601 cagtcccatc acaggggatt tttgagaccc taggctcaaa aataagcaat gaaaccatcg
      661 gtttgcttgc gtccctcacc acctaagaaa ggagcaaaaa atcttatctc ttgatttctt
      721 ccatgacata cgcctcaatc acatcatcca tcttaatatc attgtagccg tcgatcatca
      781 atccaccttc acggccgttt gtaacttcct taacgtcatc tttatagtgt ttcaagcttg
      841 cgagttcacc atcatagata acgacaccat cacggataac acggacttta gagtcacggg
      901 caaccttacc gttgataacc ataaatccac cgatagttcc cactttagac accttgaagg
      961 tttcacggat aaccgcttca ccaataactt tttcttcaaa ttctggatca agcatccctt
     1021 tcatagcttc ttccatctct tcgataacct tgtagataat gctgtgaaga cggatttcca
     1081 catcgtcagc ttctgcttgt tgacgagctt gtggtgtagg gcgtacgttg aaaccaacga
     1141 taaaggcatt tgaagcttcg gcaagggtca cgtctgattc gttgatagca ccgaccgctg
     1201 agtggacgat agtcactttg acaccttcca cgtcaatctt ttgaagtgag gcagaaaggg
     1261 cttcaacaga accttgtaca tcagccttga tgataacatt aacagatttg agttccccag
     1321 ctttaagggt atcaaagagg ttttcaaggc taacacgttg ggtagcttga cgttgtttca
     1381 tgagggcacg tttggcacgc tcttcacctg ctgcacgcgc agatttttca tcctcgtaaa
     1441 cggcaaagtg gtcacccgcc atcggtgctt cgttcaaacc tgtgatagag actggtgttg
     1501 atggtccagc aactttaaca cgacgaccaa ggtcgttggt catagcacgg acacgaccga
     1561 aggtatttcc gacaacgatt gggtcttgaa cattcaaggt accttgttgt acaagaaggg
     1621 ttgcgaccgc accttttcct ttatccaagc gcgcttcgat aaccgtaccg attgcacgca
     1681 ctgttgggtc tgccttgagt tcttggattt cagctacaag aaggactgtt tccaacaatt
     1741 cttcgatatt ttggttgaat ttagctgaga tttcaacaaa ttcagaatct ccaccccaag
     1801 cggttgacat cacaccatgc tctgccaatt caccgataac gcgttctggg ttagcacctg
     1861 gtttatcaat cttgttaatg gctacgatga ttggaacgtt agctgctttt gagtggttga
     1921 tggcttcaat agtctgaggc ataaccccgt catctgccgc tacgaccaag atcgtaatat
     1981 cggtaacaga agcaccacgc gcacgcattg atgtaaaggc cgcgtgtcct ggtgtatcaa
     2041 ggaaggtaat cttcttacca ttttccacga tttggtaggc accgatatgc tgagtaatac
     2101 cacctgcttc acctgtcgca acacgtgagt tacgaagagt atccaaaagg gttgttttac
     2161 cgtggtcaac atgtcccatg atagtaacaa ctggtggacg ctcaaccaat tcatcttcat
     2221 tgagataacc atcttcgaca aagaaacgtt cgatgtcagc attatccact tcaacctttt
     2281 gtttggcttc gataccgtaa tccaccatga ggagttcaat tgtttcccca tctaaggatt
     2341 ggttttgtgt ggccatgaca cccatcatga aaagtttctt aacgatttca gctggttcac
     2401 gtttgatacg ttttgcgatt tccgcaacgg tcataccatc tgtatattca aattctgttg
     2461 gcaattcatg gaatttacgc tccgtaacag gttttggagt ctgattacgg ttgttcttgt
     2521 tattgccttt tttgttcttt ttgttgttat tccagttact attcttttga tttctcactt
     2581 gattttgact acttcgattc ttttgttgtt ttctaggacc atcttcttca tgatcataat
     2641 cgtcacgatt tttgtctggt cgagcttgtt ttttacgacg tgtatccact gcaggttctt
     2701 ttttctcagg gacgacttca accactgctt gaacttgctg tgcttgttca gctaacttag
     2761 ccgcttcttc aaagatttcc tctggttcct tgcgtttgtt agcttgagcc aaggcttctt
     2821 tagcagcctg atactgcttg aagcgttcct cacttgaacg agcgtactct gcattttgct
     2881 ctgcttttag ggctgctgca cgggctttaa agtcaatacg tggagccgct tgatttgaac
     2941 gtttgtcctc ttgctgacgg cgctcatttc tacgtttttg ctgaccttgc tgcttcttag
     3001 cttggtcatt aaagcgacga ttgtcgcggt tgctttgacc ttgttttcca ccattacggc
     3061 catcgttttt ctgacggttt ccgttttgtt gttggtcacg gttattgccc ttattttgct
     3121 tgcgtcgttc tgcctgctct ttagcacggg cttcacgctc agccttgaaa ttacggcttt
     3181 gcggtttcgc tgctactttt tctgttttcg gagcaactgg ctcagccggt tttgcctctt
     3241 ccttagctac agctggttta gctggctcag atttttcggt tttcttttct gcacttactt
     3301 ttggtgctgc aggttttgct tctactttcg gagcagctgc aggcttaaag ctggcagcaa
     3361 tttttgcagc gacagcttct tccacacttg atgagtggct tttcacatcc aagcccaact
     3421 cttttgcacg cgctacaact tctttacttt cttttccaag ttcttttgcg atttcgtaca
     3481 atcttttctt agacaaatca tgtcctcctc ttctattcca taagagacct cattttcttt
     3541 gtaaatccag catctgttac agccaaaacc tttctcgatt tcccgactgc tatgcttaat
     3601 tccagtgttg aaaacacggt tacaatttct acttgataat aatgactttt atcttgaatc
     3661 ttcttggcca gattgggtcc agcatcatga gctagaaaga ccaacttggc cttgccgtct
     3721 tgaatggcct tgaccaccaa ttcttcaccc gatatgatgc gccctgctcg ctgagcaagc
     3781 cccaagagat tacttatctt ttgcttattc aagtcccaac tctcttcttt tcactttgtg
     3841 atccacataa gcgatcaact cgtcataaaa gctttcttcc acttccatgc taaagctgcg
     3901 gttaaagacc ttcttctttt tcgcctctag ggcttctgca ttgtctagtt tgatataagc
     3961 gccgcggcca ttggccttgc ccgtaggatc aataaagact tgtccttcct tgttcttgac
     4021 aatgcggagc aaatcacgct tatcaatcac ttcgttagac acaacagact tgcgcaaagg
     4081 gatttttctt gttttcatct ttccctcctc tagcagcttt tattcttcta cagtatcgtt
     4141 ttctacttcc aactctactg aagcagcgtc ttccatggct tcaaattcgc tagcagactt
     4201 gatatcgata cggtaaccag tcaagtgagc cgccaagcgc acgttttgtc cacgacgacc
     4261 aatggcaaga gaaagcttgt tatctggaac aaccaccaag gcacgtttgc tgtcgttttc
     4321 atcaaagata acttggtcaa cctcagcagg agcgatggca ttgtagataa attcagctgg
     4381 atctgctacc cactcgataa catcgatatt ttcttcgatt ggtaccatgc ggtcattttt
     4441 agcatcgtaa cgagctgggt ggaatttgct agtaatcttc ttgatattag caccaccacg
     4501 tccaacgatt gtaccgatag catccacgtt tggattgtgg ctacgaacag caaccttcgt
     4561 acggtcacct gcttcacgag ccacgctcat gatttcaaca gttccatcat aaacttctgg
     4621 aatttcttgc tccatcaaac gtttgatcat ttctggatgg ctacggctaa caaagacgtt
     4681 cacaccacga gggttgtctt caaccttgta aacataaact tcgatacgat catgagaagc
     4741 aaaaacttct ccaggaattt ggtcttgttt tgacaattgg gcttcgatgc taccaaggtt
     4801 gacatagata aagcggttgt caaagcgttc tactgtacca gacatgattt cttgctcatg
     4861 ttctttgtaa gtattgtaag tgatggcacg tgtttgcttg cgcatttttt ccatgatggt
     4921 ttgtttggca gattgggctg ctacacgacc aaactcagct ggtgcttctt caaacttgat
     4981 tttgtctcca agttcataag ctgaattaat ggcaagagca tctttcaagc tgatttccaa
     5041 acggctatca aatacttcat caacaacttc acggacagta taaactgtaa agtcacctgt
     5101 tttttcgttg aagtcaatag ctacgctgtc tgactgacca tagcgtctgc gataagcgga
     5161 acgaagcgac tctactactg cgtcgatgat atcttctttt ttgattccct tgtcttcttc
     5221 caaaatgcgg aaggcctcta gcatttcttt actcatgttt tcttcacttt tgaatcctca
     5281 aaagctatcc tttctttttc tataatttaa ctgctaaacg tgcttttgat actaaactgt
     5341 atggaattcg gacggttttc ttacgcgtct tgtccatata ttccatagtc aactcgtcct
     5401 cttcgaaggc caacaaagtt ccttcaaaga ccttttgctt atcgatggct tggtagagtc
     5461 cgacatggat gtattttcca accgctccag cgacggcatc cttggttttc aaaggacgtt
     5521 ccaagcctgg actggtaatt tctaggaaat attgttctgg gaagggatct ggcttgatgg
     5581 tgtctaggac aggactgata atttctgtca agtctgccgt gtcgttcaag gtaattcctt
     5641 cgggtttatc tacaaaaata ctgagaatca tgtcactgcc aatctttcca tactcgatat
     5701 ccacgagttc aaaaggagct tctatgacag gttctacaac ttctctgact aattctacga
     5761 ttgttgcgat tgcgtccacc tcctcataag caagaggcga agatatttcc ccgcctcact
     5821 tttcatattc ttactatcag tatagcacgt ttgccaactt atgtaaagca aaagcacttg
     5881 tacagcctaa ttttaggcta tctcttaaaa ttctgcctca actcggtaga taacttgccc
     5941 cttgtttgag aatttttgct cgtattctgt catgacattg ccttcaaaat cactggcatg
     6001 caaatctagc cagacaccat tgagtttcat gccatattga gaaaagctca ctaaactgta
     6061 ctcaaacaag ccacggttat ccgtcttgaa atgaatttct ccattttcag gcaagatacg
     6121 tttgaaggtg tccaaaaagg tcttgtaggt caaacgacgc ttttcatggc gttttttcgg
     6181 ccatggatct gaaaagttca gatacaagcg atcaatctca ccgtcttcaa agtagtcagt
     6241 taagtcagaa ccatctaccc acaagagctt gatgttaggc actccaactt caagcacctt
     6301 gtccaaagcg tagctcaaaa cagacttttg aatatcaatc ccgatatagt tgatgtcagg
     6361 gttttgcttg gccatacctg aaacaaaggc accctttcca cttccaactt ccacatgaat
     6421 gggattatca ttgccaaaca agtcccgcca ttttgccttg gcttccaagg gattgaggac
     6481 cacatactgg ggatttgcct ctagtaattc tgttgcccct ttacgatttc taactctcat
     6541 cttctctttc catacttgtc tcggaaatga cgcaaaccat gaatctcccg attgacattt
     6601 tctaaatctt ggttcatata atacttggaa atctgactca aataagacaa ttgaccatac
     6661 caatacaatt tacttaatac cgtttgattg tacttgtaac cgtagtaggt caaccattcc
     6721 ttccactgat gttctgaaat ataatggcag agcatatggg ccacatcaaa catgcgatcg
     6781 gtcaagcgaa ccgaatccca atctactaaa taaatcaagc cactatctgt ctcaatccaa
     6841 ttactatgtc gtacatctcc atggacaatg gtcgcatggt cctctctaaa tcctggaata
     6901 gtctgacgta aatcagccat cacttcactg ataaaatgat ttttacgcaa agcatctgga
     6961 gccgtttcct gccaagactg tagtaaatct acaggtgttt ccatggcata gcccaaacga
     7021 ctcaattgtg tcatcaacgg acgtgagcga tgaagacggg ttaaaatatt gacgatttgc
     7081 ttacgattca tatcataggg ggtcaatatc ttgcctgtca accattcttg agcacacata
     7141 tcacgcccat ctgccaaacg acgactccat aataattgtg gagcaatttg ttctctagct
     7201 agaccaggta ggattggaga ggtgttcatt tttacaaaga tgcgcttccc atcaggatag
     7261 ctacccatat aagccttgcc acttttccca ggtatgggag tcagtgttag ctcattatca
     7321 cccaaatcca tttctttcct ccgtataaag tttccttact attctactag tttttggcct
     7381 attcgtcaac cactctaacc cgattctcaa agaaaagcct ctggattggc ttgggtatca
     7441 ttatagcttg aaaaaggtaa gcaccgtctt ctgcttacct tttcataatt tttataaata
     7501 atataagagt ggccaactgt tgtaaagcat atggactaga gtagaaaccc ataggttttg
     7561 actcttttta taagcaaagc ccagcaacaa acccccaagt agataaaaaa taaacgaagg
     7621 gacattagaa ggtccatgca tgaaagaaaa gagagaggaa gttagcacaa gccctaacca
     7681 agaattgtca aaaaccgcac cttgaagaag tcctctgaaa atgagttcct cctggatggg
     7741 gccaaaaact gaggtagtta aaatcaaaga cagggaaatt cctctgctga cttccgccaa
     7801 gtgttggacc cctgagccag aagaaacagc gaagaaatga atataaacca gcgtctcaag
     7861 tacacttaga agaaagattg caatgaaaag caaaagttgt ttcttgctta acatcccaaa
     7921 agaaatcatt tcaaactttc tagcataagc aacactcagc gaggttacaa gaagacttac
     7981 aagaatcaat gcagattcat tttgaaaaaa acccccatgg ttaatcgtat aaggaactgt
     8041 aatgacaatt attagtacta aaaatccaaa acacaaggca tgaaatttat caaaaaactc
     8101 cataaaatta tcctcctcaa caaacagact tcctacacgt cattctttag ttctagcctt
     8161 tccaaaacaa accattttat actcttcgaa aatcaaattc aaaccacgtc aacgtcgcct
     8221 tgccgtactc aagtacagcc tgcggctagt ttcctagttt gctctttgat tttcattgag
     8281 tattagtaac caaaatccga ccacatagcc agcccctatg aatatagcca ttaaagctag
     8341 catggaattt aggaaattaa aaaccaccgc agatacaaag gttagcacaa aaacattaaa
     8401 agcaatggtg tcagaagcca agactagaat atagggtgtc aaccgatcta aagttttgga
     8461 atctaggaaa aataagtgtt tatacatgat gacctcctct atggctgaaa agcaagcctt
     8521 ttgttttttt accccaagac cctatgtaga aaagtgagca aaaacgggaa ggtcgctaca
     8581 atattattga tcacatgcac cgcataggat ggataaatgc tcttggtata gcgggtcaaa
     8641 ccagcaaaga tgattccaac tgttgcaaag acgaagatat ctaacagact aggcaggctt
     8701 gaaaaatgag ggagagcaaa taaaatagaa ggaagaagca aatcaagacc aaatcgcgaa
     8761 tgcttaaaga aagcatgttg cagtaatcct ctataaatca attcttccat cagtggaacc
     8821 agaaagaaca gggctatata aatacctagc tctgcaaagt tagtcccact ataaccgatc
     8881 aatacagccc aaccttcagc agttgactga acatgtttag ctgtctgaac gttaaaagag
     8941 atctggagca ctaccactaa tactgtcaaa atcgaatacc aaagccattt ttttcttgga
     9001 atgtgaaaga gataaccatg acctgtctta accagaatcc aaatcatgac tccgctaaat
     9061 agcaaactca agaaattttg aatccagacg aaattgccaa tctgggaaga aaattgccaa
     9121 taattttgaa taataagcgt catctgagaa agaccaaata cgaaaaataa gtaagagaaa
     9181 actgcactta ttttgaatag aagttgatac tttttcatag aaatcctccc tactatgacc
     9241 tcaccttgtc aggctctact gctgtaagat taagaagaca gtttgttttt tttaaggcta
     9301 acctgactac tagataatag atacattaag gcattaaaga caatgaaaat atgtccatag
     9361 aataaaatca acctcgcatc caaaccaaga taaagtttga ttatcaaaaa gatgagcaaa
     9421 agaatttgaa accataaggt ttttccaaaa ataaatttaa agcgatttcg aatatctact
     9481 tccttgattt ttaccgccac ccctttatta gcaagaagga aaactcctgc ttcaaacaaa
     9541 ccactgtaaa gaacaagcca cccaatagat acgatagaga tttgtaaaaa tgtccctaaa
     9601 agaatatcca acacactact caagaaaata acaaaaaata atctgtattt catattaaat
     9661 acctccattc atttatttca ctaacaattt aatagagcct tctactcaaa tatcctgtca
     9721 gaaaaggata gaaagctact ttttataata cttcaagccc cacatgagca gaagcgtgat
     9781 aaacaagcag agaatacacc tatataagcg attagttgtt gatagaattc tgtttctgaa
     9841 atacctctat acaaacaaat gacaaacata aaatctgcca agccgataaa cataagttga
     9901 ttggttctag gactaaccaa atcatcattt acttatattt aagagtatct cttttatgtt
     9961 aatgtatgtt agcactgaaa agcaagacag gccaataata tttaaaatga acagtaacgg
    10021 ggttaagtct ctaaaaaaat tatctactga cactacaaga aatactatac atattatagt
    10081 cgaaactatc tttttcttat ccataattat ttactccttt cctaacaaat ccaacttatc
    10141 aatcaagagc gatttttaac ataatgtagc agcacccgtt gcaactttga caagtttagt
    10201 atatcattgt tttttaaaat ttttcatcca aatcttgaat tgtcatcgaa acctcctaaa
    10261 ttgcaaaaac ttcattagcc cctacttttt ataatacttc aaaccccaaa tgagtagaag
    10321 catgatgatc aagcaaagaa tagcaccaat ataagcgatt agttgttgat aagattctcc
    10381 tgctgtgata cctctataca aacaaataat agacataaag cctgtcaagc cgataaacat
    10441 gagttgattg gttctaggac taaccaaatc atcatcttca aactctctta atctcatttc
    10501 cctagtaaga taaacagtaa ccaaaataga agtcaagtta ataactacta aaagaaattg
    10561 gaaaactgac gaaatagaaa tagataagta gaaacaagca aaggcaactg acccaggaac
    10621 aatctcacaa agaatatgat tctttttaat caagaaaatc tttcatttct tttctttctt
    10681 tttaattaaa gaaaaataga tcataaccgc aatcatatag gctatggtat aaaatagatt
    10741 ataccaaata ctctccctaa gcggataaag aaagatggac atgatcagat acagaacgaa
    10801 aataatcagt atttttttct tcataagttt tcctcctaaa tgtgcgattt atcttagttg
    10861 agcaagaaca tttacactgc tagtatagca cttattttga ccttggatca ctcaaatcat
    10921 aaatggtcat caaaacctct tgaattgtaa aaattaaaaa agcaagcatg aaaaacatac
    10981 tttcctcttt atattgtatt gataccaact tgtttgtaga cttttcatcc tgctatcaca
    11041 tatcattttg acaggcgaaa caatattaaa gaaactcccc tgtaaattaa gctagcaaat
    11101 acaggggaga aatttatttt ttagagagta ctatccgtat cctttttgga agattttgaa
    11161 aatatttttc taattaagtc atccatataa ggaccaaata taccaactac taaaccaata
    11221 ataaaacttt taaaatccat aattaccacc aacatgttgc tgcataggct acacctccaa
    11281 gtatagctcc acctgcagca ccagttacac ctattcctat agcaaatggt cccaatagaa
    11341 atgtcaaacc gttgttgcac acccatcaat tgcgccatat gcaacccctg ctgcacaact
    11401 aatttttctt ccccaatcaa tatctccacc ttcaacgcaa gcaagcattt cattatccat
    11461 aacagaaaat tgtgacatca tttttgtatt catatagtat gtcacttttc agttacggaa
    11521 caagtttaat ataaaaatta tcaaaaaaac ataggcaata aagagaaaaa ttaatttatc
    11581 atagattaga aataatatga caaaacaatt caatgatgtt aattcaatag tcttttgttt
    11641 tttatcagag atacttatgg atagataaat aagataggtt tgaaaagcga agagaataat
    11701 aaagaatata gccttcataa aatttagctt tcatttttat gatgtagcgg tataggctaa
    11761 atatccacaa accactgctc ctccaattcc tcctattgca gcgccccatg gtcctagaat
    11821 tttcccatat ttcactccac ccgctgcaca acctaaagca gcaactacag ctgctcctcc
    11881 aacacctgct ttggcaaaat ctccccaatt gcatccgcca ccttcaacgc aagcaagcat
    11941 ttcagtatcc ataacagaaa attgtgacat catttttgta tccatgacaa atactccttt
    12001 tttaaaaaac taaaataaat cagaatagaa tcctcataat tttactataa gtcttaccaa
    12061 cttagtccca atttatcacc aaccatacct cctaagcatg ttaatccacc cccaattgca
    12121 ccaatgtgtg ctccaacaaa tgcaccagca agtccagcta ctcctaaagt ggccaaacct
    12181 gctccagttc caccagttat aattcccgta gtgactcctg taatcagtgc attttgacaa
    12241 tcagtggagc tatacccccc ttcaactttc gcaagcattt cagtatccat aacctctaac
    12301 tgtgacaaca tttttgtatt catgatgaat acctcctttt tattttcaat ttgttaccaa
    12361 agtcttaaat tcaataaaca aatagatttt ttatagtatc tttttgattt tcttaaaaaa
    12421 gtatatacgt ctactatctt cttaaaggta gcagtaccta ttttttagtc taagatttca
    12481 ataatcttga gtatctaaaa tatcttaatt tcgttattct ccttgcaata aaaagtttta
    12541 ctatactatt tattaacttg cagaaagcaa aaaatattag taaataatag tttatagtta
    12601 agttttttat tcctaccaat ccatcaacta agtaaagcat caacgattac ataaacgatt
    12661 gataatataa ttaaaatttt gctaactatc ttattctcat cattcttaga taactttgat
    12721 attttgtaag taagtaaata agacagtaaa ttaatagcga taataatact atatttaaga
    12781 atcataatct tacaaagagg acataattcc tgaacctaca caaataagtg ttgctgctcc
    12841 cccagttatc ggaccagtcg cagcagctaa tagtactgct ccaatacaac caccgattgc
    12901 agatcctaaa ttgcctcttc ctccactaac tatttcgagt tcttcattat ccataatctc
    12961 aaattgttcc atcatttttg tattcatgac aaatactcct ttttcttttt ttatttttgt
    13021 cttgttgtaa ctttgataag tttagtatat catcgttttt taaaattttt catccagatc
    13081 ttgaattgtc atcgaaacgt cttgaattag cttttttatt tcaagccacc tctaaatgtt
    13141 taaaaaaaat aatttctaat cactttttta ccattcagga agttttaatg actattcaag
    13201 atttcataaa atatgaactt agttttatga cataatagac ctatccacta tatgaaaaga
    13261 attgccaatg acttcttata aacgtacatt tgttcctcaa atagatgcga gagactgtag
    13321 tgtcgctgcc ttagcctcga ttgctaaatt ctatggttca gatttttctc tagctcactt
    13381 gagagaactt gcaaagacca ataaagaagg gacgactgct cttggcattg taaaagccgc
    13441 tgatgaaatg ggctttgaaa caagacctgt tcaagcagat aaaacgctct ttgacatgag
    13501 tgatgtcccc tatccattta tcgttcacgt taacaaagaa ggaaaactcc aacattacta
    13561 tgttgtctat caaacaaaga aagactatct gattattggt gatcctgacc cttctgtaaa
    13621 aatcactaaa atgtcaaaag aacgcttttt ctatgaatgg actggagtag ctatttttct
    13681 agctcccaaa cccagctatc aaccccataa agataaaaag aatggtctac taagcaagct
    13741 tccttcctct gattttcaaa caaaaatctc tcattgctta cattgttctc tcaagcttat
    13801 tagtcactat tatcaatata ggtggttctt actatctcca aggaatctta gatgaataca
    13861 tcccaaatca gatgaaatca actttaggaa tcatctcagt tggtctggtt atcacctata
    13921 tcctccaaca agtcatgagc ttctccagag attatctcct aaccgttctg agccagagat
    13981 taagcattga tgtgatttta tcctatattc gccatatttt tgaacttccc atgtctttct
    14041 ttgcgacacg tcgtacagga gaaatcattt cacggttcac agatgctaac tctattatag
    14101 atgccttggc ttctaccatt ctttctcttt tcctggatgt ttctattctg attcttgtag
    14161 ggggcgtctt actggcacaa aaccctaatc tcttccttct ttctcttatt tctattccta
    14221 tatacatgtt catcatcttt tcttttatga agcctttcga aaaaatgaac catgatgtca
    14281 tgcaaagtaa ttctatggtt agctctgcca ttatcgaaga tatcaacggg attgaaacta
    14341 taaagtcgct cacaagtgaa gaaaatcgct atcaaaatat agacagcgaa tttgtagatt
    14401 atttggaaaa atcctttaag ctcagtaaat attctatttt acaaacaagt ttaaagcagg
    14461 gaacaaaatt agttctgaat atccttatcc tatggtttgg cgctcaatta gtcatgtcga
    14521 gtaaaatttc tatcggtcag ctgattacct ttaacacact tctttcttac tttacaactc
    14581 ctatggaaaa tattatcaac ctccaaacca aactccaatc tgcgaaggtt gctaataacc
    14641 gtttgaacga agtctatcta gtcgaatctg aatttcaagt tcaagaaaac cctgttcatt
    14701 cacatttttt gatgggcgat attgaatttg atgacctttc ttataagtat ggttttggac
    14761 gagatacctt aacagatatt aatctcacga ttaaacaagg agataaggtt agcctagttg
    14821 gagttagtgg ttctggtaaa acaactttag ccaaaatgat tgtcaatttc tttgaaccct
    14881 acaaaggaca tatttccatc aatcatcagg atattaaaaa cattgataaa aaagtcttgc
    14941 gccgtcatat taattaccta ccacaacaag cctatatctt taatggctct atcttggaaa
    15001 acttaacttt gggcggtaat catatgatta gccaagaaga tattctaaga gcttgtgaat
    15061 tagctgaaat tcgtcaagac attgaaagaa tgcctatggg ctatcaaact cagctctctg
    15121 atggagctgg tctatcagga ggacaaaagc aacgaatagc cctcgctcgt gctcttttaa
    15181 ctaaatctcc tgttttaata ctagacgaag ctactagcgg tcttgatgtc ttgactgaga
    15241 aaaaggttat agataatctt atgtctctaa ctgataaaac cattctcttt gtagcccatc
    15301 gtctcagtat agccgaacga accaaccgtg tcattgttct tgaccagggg aaaatcattg
    15361 aagttggtag tcaccaagag ttaatgcagg cgcaaggttt ctaccatcat ctattcaata
    15421 aataaggaga atgtcatgaa tcctaatctt tttagaagcg tcgagtttta tcagagacgt
    15481 taccataact atgcgacagt gttaattata cctctttcat tactatttac tttcatcttg
    15541 attttctccc ttgttgccac aaaagaaatt actgttactt cccaaggaga aatcgcccct
    15601 acaagtgtca ttgcctccat tcagtcaacc agtgataatc ctatcctagc taatcattta
    15661 gtggcaaatc aagtagttga aaaaggggac ttactcatca aatactctga aacaatggaa
    15721 gaaagtcaga aaactgcctt agcaactcaa ttacaaagac ttgagaagca aaaagaagga
    15781 cttggaattt tgaaacaaag cttagaaaaa gcgactgatc ttttttctgg cgaggatgaa
    15841 tttggctacc ataatacctt tatgaatttt actaaacaat cccatgatat tgaactgggt
    15901 atcacaaaga ctaacaccga agtttcaaat caagctaatc tttccaatag cagttcatca
    15961 gctattgaac aagaaattac aaaagttcaa caacaaattg gagaatatca agagttgaga
    16021 gatgctatca taaataacag agcacgctta ccaactggca atccgcacca gtcaattttg
    16081 aatcgttatc ttgtagcctc acaaggacaa acacaaggaa cggcagagga gccattttta
    16141 tctcaaatta atcaaagtat tgcaggtctg gaatcatcta tcgcaagcct caaaattcag
    16201 caagctggta tcggaagtgt agcaacttat gataacagtt tagcaaccaa aattgaagta
    16261 ctccgcactc agtttttaca gacagcctca cagcaacaac taactgtgga gaatcaatta
    16321 acagaattaa aagtacaact agatcaagcc acacagcgtt tggaaaacaa taccttaacc
    16381 tccccaagta aaggtatcgt tcatctgaac agcgaatttg aaggtaaaaa tagaattcca
    16441 actggtacag aaattgctca aatattccct gtcatcacag atacaagaga agtactaatc
    16501 acttactacg tatcttctga ctatctacct ctactagata aaggacaaac tgtaagatta
    16561 aaactggaga agattggaaa tcacggcacc accatcatcg gccaacttca gacaattgat
    16621 caaactccta ccagaacaga gcaaggaaat ctctttaaat taaccgctct tgcaaaacta
    16681 tctaacgagg atagtaaact catccaatat ggcttacaag gtcgcgtcac tagtgtaact
    16741 acaaagaaaa catattttga ttatttcaaa gataaaattt taacacattc tgattaattt
    16801 tcagataaca ctctataact atttattatc ttatcaaaaa ggagaatcat aacatggata
    16861 agaaacaaaa cctaacttca tttcaagaac taacaactac cgaactcaac caaattacag
    16921 gtggaggatt gtgggaagat ttattatata acattaatag atatgctcat tacatcacat
    16981 aagaacttca tcatccaata caactataaa aaaataagac cgagaaacaa gtactctcgg
    17041 tcttattttt catcattctg tatgtatcac agtaagtacc tgacgaaaga cttgattttg
    17101 acaggtggta tttagactgg tattaggatg gctttccaca atcttcatga cggtatagag
    17161 accaactcct ctctcctccc ctttagaact ggctccaaag gagaagattt cagaaatatc
    17221 gatgccctct tctttgatgg agttttcgat gataaaggtc tcctgtgctc cattttttaa
    17281 aaaggcgatt gaaacatgag gttgactagc ttccacactg gcttcaatag cattgtcaca
    17341 aaggatagac acaatggtta gaaaatcaag tagactcatc ccctcgacct gaatctcctc
    17401 aggaacttcg acattaaaga caatgttctt atctctggct tttaaaaatt tccctgctag
    17461 aagacttttg agggctttat cacgaatatt taccaatctg cccaggtcat atttattgtt
    17521 ctgcaatttc tgactggaat cctttaagac ggagccatag acctctttta tctgctccat
    17581 atcctcctct tcaatgccca gacgtaagct agtcaagagg ttggtataat catgacgaaa
    17641 gctccgtact tccttgtaaa gctcctctat atgccgacta tagcgttcca tatctctata
    17701 gcgcagggcc tgctcttgtt ccaatctctc atagagtttt tccttcaaat aggtatccaa
    17761 tttcttgata acccccataa aaaagagtag gtaaaagact aggatgagat ggcgaacagt
    17821 ctttgattga atactttgtt catattcaaa aaaagacaga ctttccatga ctaaatagta
    17881 gccacccatt atccagttaa tctgagtcag ggacttttga aaggctttat cgagaatctc
    17941 ctttctcaag ctagtaaaat cgtagtccaa ccatttcaaa aaagctagag aaatgaagaa
    18001 attgaaaatt attatacata acccagtaaa tgagtagcca tcatatactt gcccttgtcc
    18061 caaaaatgga agcacaaaat aggagactcc tctataaaag agattcacca atatcattgg
    18121 aaagagacca taaaagaaaa ggagtttttt aggaagccct ctcaataata agaaagataa
    18181 gcctatgccg tacaagggtt ccataaaata agataggtaa acatttccta ctatatagct
    18241 aatcatcaca aaaacaaagg ccaacagtat cttcaaaaga aaggccttaa aaatcctctc
    18301 gaaagtaaga tcaattccat ccaccttaaa gaagatgaca atttctagtc cattagtaac
    18361 aagtgtatac aacaatatcc aagcaatgtt cataaattct cctagctcag tgtaatttat
    18421 tgatggcctc agacacttcc ctgaccttat aacgggcgat tagacaactt ccaccattgg
    18481 gaaagaaaag cagtttttct ttcttatcca aatgcaccac atttgcagga ttgatgagaa
    18541 aagagcggtg gcactgcaag agacggggct cctgcttgaa aacctcctct aaactcgctg
    18601 taaattccag cctgtctgtc ttggtataga gaataacacg atgggctctg ggcgacgttt
    18661 cgagatagta aacctcttta aaaggatact gaaattgggc aaattttgat ttaaagtaaa
    18721 agcaatcttc cgccagactt ttactatctt gactattggc atagaggagg gctgtctcga
    18781 tccgagattc aaactcctct gctgacaagg ccttatcaat gtagttcaaa gcagacactt
    18841 ggtagcgaaa agacaggggc ataaactccg agtgagtcgt cacaaagaca atcagggcat
    18901 aaggatcccg atcccgaatc tttctagcca cttccagtcc cttcatctct tcatttcgaa
    18961 tctcaatatc caaaaagaat agctgatggg cccccttctc atgcacttca gccagcagtt
    19021 ggtccggctt gccaaatacc tcaaaagagc taggaatgat atgatgtgct ttcaaaagtt
    19081 tctcaatcgt cgtttcaatt ctagtctgtt gggaaaaatc atcctctaaa acaaatattc
    19141 tcatcttttt actctcctta ttttaaccat ttttgacgaa tttctctata gcgacgtctg
    19201 gcatacttga cagcgtattg accttcttct ccgagaaaaa ggattctttc ttgaaaatca
    19261 atcgatttta atttatcaag attaaccaaa caatttcgat gacatctcat caaggtttcc
    19321 ccacattggt tctcaagatt acttaaattt tgaagcatat taaaactagc ttcttctgta
    19381 acaatctgta cggtatgggc tttagttgga tgtgtttgga tatagtagat atcgtcgacg
    19441 tttactttat agactgtttt ctgcgtctga ataatcatat gtttcattgg attctcctcc
    19501 taagaaaaga ataacagaaa aggtctagcc caagcccacc caatcctaaa cagtctttga
    19561 aagctcctaa aaggattatt tttctacaac atcatgatga gcttacaact attttatcat
    19621 aaaattcact cagcgcaatt caagacattt caatgacaat taaagatttg aataaaaaaa
    19681 aagaattaaa aatgataaac taacagtatc aaaacacagt tgccagaagt aagactggca
    19741 cccagattaa aggaggcatt cccatgacag atacagaccc catcaaaaga gctcatactt
    19801 tgattactga cttaaacaaa gcctaccaag catgcaaaca ggcaagcgct gacgacgtcc
    19861 gctttcagga gcaattaaac tctattcttg gttttctagc caaggctgaa acagtggata
    19921 atcgattctt gattgaacta gaaaaatttt accagacttc cagtcttctc atgggactca
    19981 gcgctcttga tccagatgct ccaactcgcg ccgcttggcg ggcctatgac cgcttccact
    20041 ttgaccaagt caagaccaag ttaatactca atgaaaatca aagagcaaac taggaagcta
    20101 gccgcaagct gtacttgagt acggtaaggc gacgctgacg tggtttgaag agattttcga
    20161 agagtataag tctctacgga ccaaccatta tcctgtagta acaaaaaaga agctgagaca
    20221 gactgaactc aacttctttt ttagtgtcgt atcagcaatg ttagtcctgc atctgacgtt
    20281 ttacctgata aggcaaatac aagactagta aaaccaaacc agctcctagt aaggctagaa
    20341 gggccagttt ttcttggaag gtaatcaacc caacaactaa ttccacaagt aaaacaaaac
    20401 tggtcaatcc tcgaactacc tcctgtaagc ctttttcctt ttgccccttg tccagcggaa
    20461 agagttgggt caaatactgg tagtcaaagg catgatagag ggccagcaac tggaagagca
    20521 agaggtagtt aaagagaact accactgctg tcgcaatcca agcttgctcg ataaaaacct
    20581 gcgccagtac ggaaagtaag agaagacgaa gactgagagc aaagaggtcg ccatttcgca
    20641 gataagaacg cagatagaga ttttgccaaa tcttcccagg caccttctga acagccttta
    20701 aaataaagtc cagataggca cgacgcttaa cgctgtttga aattcccttg acctgcgtaa
    20761 agagggcaaa gaaacgaagc aagacttgct tacgcttgct ttcttgagaa ataagatagt
    20821 cccagtccag tccagtttca gtgaaaaatt tgctggcctt ttgacaaaag tggaaatatt
    20881 ttcctacccc caataaaagc acatagagca gaaaaactgg caagccataa cccattgcta
    20941 aaaataaagg cgcaaataaa agcaagaaga gggtctggac aaaaatccaa aagactaggg
    21001 aaatgccagt ttgacgcttg agatggagct taatttcctc ttctccaact aagagaaaga
    21061 gcttgtctgg agcctccata taggtggcaa ttcctcccca aagtaaaagt aaaacagacg
    21121 taattcctac aaacaaaagg ataggccaat gattttcagg aaaatgttgt aagagttgac
    21181 tgtactggta ggctagaaag cccaacagga caagcaggaa caagacaaag tggtcattga
    21241 gcacatagcg cagataacca agacactcct tacgaaaggc ctgctttctc tttaaaaaca
    21301 agtctttcat aggtcctcct cttttgtcag agccaagtaa atatcattca aactagcctc
    21361 aggcatatca aaggcttcgc gtagttgcag gagattgcct ttggcacgca cctctccctt
    21421 gtgaagaatg acaaaggcat cacacatctt ctccgccgaa tccagcacgt gggtactcat
    21481 gagaatagac ttgccctttt gcttctccac ttccaaaagc tgaatcaaat cagaaatagc
    21541 tagcggatca agaccaagga aaggctcatc cacgatgaaa agacttggat ccaccacaaa
    21601 agcacagata atcatgacct tctgcttcat cccttttgaa aaatgaacag ggaaccagtc
    21661 taatttctgg tccagacgga acatctttaa caagggctct actcgttcga aagccacttt
    21721 ttgctcaata ccgtaagcca tagcaaccgt ttcgatatgc tctctgaggg tcaattcctc
    21781 atacagacta ggcgtctcag gaatgtagcc aatctgcttg cggtagctag tcgcatctcc
    21841 ttgcatagtc aggccattga tattgatgga gccactataa ggtgccaaca gaccgataat
    21901 ctcattgatc gtcgttgatt tcccagcacc attgagacca atcaaaccga ccaactgccc
    21961 actttcaaca gtaaaggaca catctttcaa aacaggaaca tgaacatagc cacctgtcag
    22021 gtttttaatt tctaacatat tttctccaaa tctggtataa tgtagctata ttatatcaaa
    22081 attcagtaca gtagaggtag attttatgtc agattgcatt ttttgtaaaa tcatcgcagg
    22141 ggaaattcct gcttcaaaag tatatgaaga tgagcaggtc cttgcctttc ttgatatctc
    22201 tcaagtaaca ctaggacaca ccttggtcgt gccaaaagaa cactatcgca atcttttgga
    22261 gatggatgct acgagcgcca gccaactctt tgcccaagta ccaaaagtag ctcaaaaagt
    22321 catgaaagtc actaaggctg ctggtatgaa tatcatttcc aactgtgaag aagtcgctgg
    22381 tcaaacagtt tttcatactc acgttcacct tgtgcctcgc tacagtgctg acgatgacct
    22441 caagattgat tttatcgccc acgaaccaga ctttgacaaa cttgctcaag tcgctgaaac
    22501 catcaaaaac gcttaaggag ttgctatgaa attatccaac ctactgctat ttgcaggagc
    22561 tgcagccgga agttatctgg ttacaaaaaa tcgccaaacc atcacagatg aagtcttgaa
    22621 taccactgac cgcgttcaag ctatcaagga cgatgtggat attatccaaa acagcctgca
    22681 aatcattaac cagcaaaaag aacttatcaa ggaataccaa gaagacttga cttacaagtt
    22741 taaggtcttg gaaaaggata tccagactag actagctgtg ataaaagaaa tgcagggaac
    22801 tgaagataag taaaaagagc cccccggctc ttttttaaaa cgtaaacaag ttatagtgaa
    22861 ttgaatttag aatagcacac catagtttct aaagcattgt tagagattac tttaaaatcc
    22921 tcttatcaat ttgttcatat tctattcaat ttgttcatat tctatttcaa tctattatat
    22981 atatcccatc aactatgcat cataatttag gtaactcata ctcaataaaa atcaaaaagc
    23041 aaactagaaa gctaggcaca gactgctcaa aacaccgttt t
//
LOCUS       DBERDK010000027        22764 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.027, whole genome shotgun sequence.
ACCESSION   DBERDK010000027 DBERDK010000000
VERSION     DBERDK010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 22764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22764
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.027"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            308..2461
                     /gene="comA"
                     /locus_tag="ACL73L_001074"
     CDS             308..2461
                     /gene="comA"
                     /locus_tag="ACL73L_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter ComA"
                     /protein_id="HGS0772069.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKT
                     TMDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKLTKLPRERFEEEWTGVTLFMAPSPDYKPHKEQKNGLLSFIPI
                     LVKQRGLIANIVLATLLVTVINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYIL
                     QQILSYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSII
                     DALASTILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNR
                     DTMEANAVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQK
                     ALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQT
                     AQVANNRLNEVYLVASEFEEKKTVEDLSLMKGDMTFKQVHYKYGYGRDVLSDINLTVP
                     QGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLGGVNLNQIDKKALRQYINYLPQQ
                     PYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISG
                     GQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLIALDKTLIFIAHRLTIAE
                     RTEKVVVLDQGKIVEEGKHADLLAQGGFYAHLVNS"
     gene            2474..3823
                     /gene="comB"
                     /locus_tag="ACL73L_001075"
     CDS             2474..3823
                     /gene="comB"
                     /locus_tag="ACL73L_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence pheromone export protein ComB"
                     /protein_id="HGS0772070.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQAEIGNLISQTEAKIRDYQTAKSAIETGTSLAGQNLAYSLYQSYK
                     SQGEENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKITASEDGVLHLNPETSDSSMVAEG
                     ALLAQLYPSLEREGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAGNQLFLDSTITSID
                     ATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            3993..4700
                     /gene="purC"
                     /locus_tag="ACL73L_001076"
     CDS             3993..4700
                     /gene="purC"
                     /locus_tag="ACL73L_001076"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HGS0772071.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAG
                     KGVLNNQISSFIFEKLNAAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIADDQQIAYLKEETRRINEL
                     LKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQGLK"
     gene            4757..8482
                     /locus_tag="ACL73L_001077"
     CDS             4757..8482
                     /locus_tag="ACL73L_001077"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HGS0772072.1"
                     /translation="MDKRIFVEKKADFQVKSESLVRELQHNLGLSSLKSIRIVQVYDV
                     FDLVEDLFAPAEKHIFSEQVTDHVLDEVSVQADLANYAFFAIESLPGQFDQRAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDATELEAVKNYLLNPVDSRFKDITIGIAKQEFS
                     ESDKTIPKLPFFESYTAEDFARYKAEQGMAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTATHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDTFVA
                     ECNKENIDAVVVATVTEKPNLVMHWNGETIVDLERRFLDINGVRVVVDAKVVDKDVKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTIFDCSVGRSTVNHPLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNPYVAEWSPYHGAAYAVIEATARLVAVGANWSKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFAQFEASQADHKVT
                     SASAVKYGGVVESLALATFGNYIGAEVTLPELKTALTAQLGGFVFTSPEEIAGVEKIG
                     QTKADFTLTVNGVKLDGHKLDSAFQGTLEEVYPTEFTQAKELEEVPAVASDVVIKAKE
                     KVEKPVVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDK
                     TNILFFAGGFSAADEPDGSAKFIVNILLNEKVRVAIDSFIARGGLIIGICNGFQALVK
                     SGLLPYGNFEDANNTSPTLFYNDANQHVAKMVETRIANTNSPWLAGVQVGDIHAIPVS
                     HGEGKFVVTAEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            8575..10017
                     /gene="purF"
                     /locus_tag="ACL73L_001078"
     CDS             8575..10017
                     /gene="purF"
                     /locus_tag="ACL73L_001078"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HGS0772073.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGTGAIGHVRYATAGEASVDNIQPFLFR
                     FHDMQFGLAHNGNLTNAASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWI
                     RDLKPGEIVIIDDEGIQYDSYTDDTQLAVCSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETRQIIGADSMTYLSIDGLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKTSFYK"
     gene            10054..11076
                     /gene="purM"
                     /locus_tag="ACL73L_001079"
     CDS             10054..11076
                     /gene="purM"
                     /locus_tag="ACL73L_001079"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HGS0772074.1"
                     /translation="MTNKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLVSSGIHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKALEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            11073..11618
                     /gene="purN"
                     /locus_tag="ACL73L_001080"
     CDS             11073..11618
                     /gene="purN"
                     /locus_tag="ACL73L_001080"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HGS0772075.1"
                     /translation="MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKTDYEAALVELLEEHQIDLVCLAGYMKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYPEVVKALFTD"
     gene            11702..12211
                     /locus_tag="ACL73L_001081"
     CDS             11702..12211
                     /locus_tag="ACL73L_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HGS0772076.1"
                     /translation="MLESKKTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIPFSEPLIVDGKIVFAEMLFNLISFIPLGVCFPLIKTNLSSLRIVGTGFLISLLFEC
                     LQYILAIGITDITDLTLNTLGVCVGLLIYQIFIRVFKSQTRKWINILGMLSLGFAYLV
                     LLLLHLIGV"
     gene            12236..13783
                     /gene="purH"
                     /locus_tag="ACL73L_001082"
     CDS             12236..13783
                     /gene="purH"
                     /locus_tag="ACL73L_001082"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HGS0772077.1"
                     /translation="MTKRVLISVSDKAGIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     ETIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAANENQIELIDLVVVNL
                     YPFKETILKPDVTYADAVENIDIGGPSMLRSAAKNHASVTVVVDPADYTVVLDELSAN
                     GETTYETRQRLAAKVFRHTAAYDALIAEYFTAQVGESKPEKLTLTYDLKQAMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDRPTVVALKHM
                     NPCGIGQADDIETAWDYAYESDPVSIFGGIAVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQV
                     VTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQA
                     KDRLDGAVLASDAFFPFADNVEEIAKAGIKAIIQPGGSVRDQESIEAADKYDLTMVFT
                     GVRHFRH"
     gene            13905..15167
                     /gene="purD"
                     /locus_tag="ACL73L_001083"
     CDS             13905..15167
                     /gene="purD"
                     /locus_tag="ACL73L_001083"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HGS0772078.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLEL
                     VNISISEHSKLIDFAKTNDVAWTFIGPDDALAAGIVDDFNQAGLKAFGPTRAAAELEW
                     SKDFAKEIMVKYGVPTATYGTFSDFEEAKAYIENHGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAHEMLLDNKFGDSGARVVIEEFLEGEEFSLFAFVNGDKFYIMPMAQDHKRAY
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPVLEGVIKEGRPYLGVLYAGLILT
                     ADGPKVIEFNARFGDPETQIILPRLTSDFAQNITDILDSKEPNIMWTDKGVTLGVVVA
                     SKGYPLDYERGVELPAKTEGDVITYYAGAKFAENSRALLSNGGRVYMLVTTADTVKEA
                     QASIYQELSQQKIKGLFYRTDIGSKAIK"
     gene            15424..15540
                     /locus_tag="ACL73L_001084"
     CDS             15424..15540
                     /locus_tag="ACL73L_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HGS0772079.1"
                     /translation="MKERLDDNPIVQGNWKTLGFQFRHETPLVAAAVPHKLR"
     gene            15570..16058
                     /gene="purE"
                     /locus_tag="ACL73L_001085"
     CDS             15570..16058
                     /gene="purE"
                     /locus_tag="ACL73L_001085"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009567721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HGS0772080.1"
                     /translation="MKPVISIIMGSKSDWATMQKTAEVLDRFGVAYEKKVISAHRTPD
                     LMFKHAEEARSRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALFALRLLSVEDRSIADALANFAEEQGKIAEESSNE
                     LI"
     gene            16045..17136
                     /gene="purK"
                     /locus_tag="ACL73L_001086"
     CDS             16045..17136
                     /gene="purK"
                     /locus_tag="ACL73L_001086"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HGS0772081.1"
                     /translation="MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPASRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAQVTVAPYKVVTSSLDLADIDLSKNYVLKTATGGYDGHGQKVIRSEAD
                     LEAAYALADSADCVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPA
                     RISESLADKAKAMAVRIAEQLNLSGTLCVEMFATDDDVIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPVIKLHAPAVMLNVLGQHVEAAEQYVTENSSAHLHMYGKIE
                     AKHNRKMGHVTLFSDVPDSVDELGEGIDF"
     gene            17146..17373
                     /locus_tag="ACL73L_001087"
     CDS             17146..17373
                     /locus_tag="ACL73L_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HGS0772082.1"
                     /translation="MIQIIVNTFIEKDKTGAVVEVLYASADQDKVQAKYEELAAQYPE
                     NYLAIYDVPLDTDLNTLDHYPSVFIEKEEFE"
     gene            17436..18734
                     /gene="purB"
                     /locus_tag="ACL73L_001088"
     CDS             17436..18734
                     /gene="purB"
                     /locus_tag="ACL73L_001088"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HGS0772083.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRAQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPL
                     LEADSEVTSRLTQEEIDEIFNPVYYTKRVDDIFERLGLGD"
     gene            complement(18789..22673)
                     /gene="strH"
                     /locus_tag="ACL73L_001089"
     CDS             complement(18789..22673)
                     /gene="strH"
                     /locus_tag="ACL73L_001089"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored beta-N-acetylhexosaminidase StrH"
                     /protein_id="HGS0772084.1"
                     /translation="MKHEKQQRFSIRKYAVGAASVLIGFAFQAQTVAADGVTPTTTEN
                     QPTIHTVSDSPQSSENRTEETPKAELQPEAPKTVETETPATDKVASLPKTEEKPQEEV
                     SSTPSDKAEVVTPTSAEKETANKKAEEASPKKDKPAKKDEAKAEADKPATEAGKERAA
                     TVSEKLAKKKIVSIDAGRKYFSPEQLKEIIDKAKHYGYTDLHLLVGNDGLRFMLDDMS
                     ITANGKTYASDDVKRAIEKGTNDYYNDPNGNHLTESQMTDLINYAKDKGIGLIPTVNS
                     PGHMDAILNAMKELGIQNPNFSYFGKESARTVDLDNEQAVAFTKALIDKYAAYFAKKT
                     EIFNIGLDEYANDATDAKGWSVLQADKYYPNEGYPVKGYEKFIAYANDLARIVKSHGL
                     KPMAFNDGIYYNSDTSFGSFDKDIIVSMWTGGWGGYDVASSKLLAEKGHQILNTNDAW
                     YYVLGRNADGQGWYNLDQGLNGIKNTPITSVPKTEGADIPIIGGMVAAWADTPSARYS
                     PSHLFKLMRHFANANAEYFAADYESAEQALNEVPKDLNRYTAESVAAVKEAEKAIRSL
                     DSNLSRAQQDTIDQAIAKLQETVNNLTLTPEAQKEEEAKREVEKLAKNKVISIDAGRK
                     YFTLDQLKRIVDKASELGYSDVHLLLGNDGLRFLLNDMTITANGKTYASDDVKKAIIE
                     GTKAYYDDPNGTALTQAEVTELIEYAKSKDIGLIPAINSPGHMDAMLVAMEKLGIKNP
                     QAHFDKVSKTTMDLKNEEAMNFVKALIGKYMDFFAGKTKIFNFGTDEYANDATSAQGW
                     YYLKWYQLYGKFAEYANTLAAMAKERGLQPMAFNDGFYYEDKDDVQFDKDVLISYWSK
                     GWWGYNLASPQYLASKGYKFLNTNGDWYYILGQKPEDGGGFLKKAIENTGKTPFNQLA
                     STKYPEVDLPTVGSMLSIWADRPSAEYKEEEIFELMTAFADHNKDYFRANYNALREEL
                     AKIPTNLEGYSKESLEALDAAKTALNYNLNRNKQAELDTLVANLKAALQGLKPAATHS
                     GSLDENEVAANVETRPELITRTEEIPFEVIKKENPNLPAGQENIITAGVKGERTHYIS
                     VLTENGKTTETVLDSQVTKEVINQVVEVGAPVTHKGDESGLAPTTEVKPRLDIQKEEI
                     PFTTVTRENPLLLKGKTQVITKGVNGHRSNFYSVSTSADGKEVKTLVNSVVAQEAVTQ
                     IVEVGTMVTHVGDENGQAAIAEEKPKLEIPRQPAPSTAPAEESKALPQDPAPVVTEKK
                     LPETGTHDSAGLVVAGLMSTLAAYGLTKRKED"
BASE COUNT         6132 a         4575 c         5493 g         6564 t
ORIGIN      
        1 tattttctag gtagaacgtt tgttctgcct atttttttat ccaaaaagtg cagttgggag
       61 ggagataggc tcatttggga aggaagtcca gtttttgttt agtgattggg gtaagatagt
      121 tgttatcaga tgagtttata ctcttcgaaa atcaaattca aaccacgtca acgtcgcctt
      181 gccgtatata tgtgactgac ttcgtcagtc ctatctacaa cctcaaaaca gtgttttgag
      241 cagcctgcgg ctagtttcct agtttgctct ttgattttca ttgagtatta gggaaaagga
      301 gatgaatatg aaatttggga aacgtcacta tcgtccgcaa gtggatcaga tggactgcgg
      361 tgtagcttca ttagccatgg tttttggcta ctatggtagt tattattttt tggctcactt
      421 gcgagaattg gctaagacga ccatggatgg gacgacggct ttgggcttgg tcaaggtggc
      481 agaggagatt ggttttgaga cgcgagccat taaggcggat atgacgcttt ttgacttgcc
      541 ggatttgact tttccttttg ttgcccatgt gcttaaggaa gggaaattgc tccactacta
      601 tgtggtgact gggcaggata aggatagcat tcatattgcc gatccagatc ccggggtgaa
      661 gttgactaaa ctgccacgtg agcgttttga ggaagaatgg acaggagtga ctctttttat
      721 ggcacctagt ccagactata agcctcataa ggaacaaaaa aatggtctgc tctcttttat
      781 ccctatatta gtgaagcagc gtggcttgat tgccaatatc gttttggcaa cactcttggt
      841 aaccgtgatt aacattgtgg gttcttatta tctgcagtct atcattgata cctatgtgcc
      901 agatcagatg cgttcgacac tagggattat ttctattggg ctagtcatcg tctacatcct
      961 ccagcaaatc ttgtcttacg ctcaggagta tctcttgctt gttttggggc aacgcttgtc
     1021 gattgacgtg attttgtcct atatcaagca tgtttttcac ctccctatgt ccttctttgc
     1081 gacacgcagg acaggggaga tcgtgtctcg ttttacagat gctaacagta tcatcgatgc
     1141 gctggcttcg accatccttt cgattttcct agatgtgtca acggttgtca ttatttccct
     1201 tgttctattt tcacaaaata ccaatctctt tttcatgact ttattggcgc ttcctatcta
     1261 cacagtgatt atctttgcct ttatgaagcc gtttgaaaag atgaatcggg ataccatgga
     1321 agccaatgcg gttctgtctt cttctatcat tgaggacatc aacggtattg agactatcaa
     1381 gtccttgacc agtgaaagtc agcgttacca aaaaattgac aaggaatttg tggattatct
     1441 gaagaaatcc tttacctata gtcgagcaga gagtcagcaa aaggctctga aaaaggttgc
     1501 ccatctcttg cttaatgtcg gcattctctg gatgggggct gttctggtca tggatggcaa
     1561 gatgagtttg gggcagttga ttacctataa taccttgctg gtttacttta ccaatccttt
     1621 ggaaaatatc atcaatctgc aaaccaagct tcagacagcg caggttgcca ataaccgtct
     1681 aaatgaagtg tatctagtag cttctgagtt tgaggagaag aaaacagttg aggatttgag
     1741 cttgatgaag ggagatatga ccttcaagca ggttcattac aagtatggct atggtcgaga
     1801 cgtcttgtcg gatatcaatt taaccgttcc ccaagggtct aaggtggctt ttgtggggat
     1861 ttcagggtca ggtaagacga ctttggccaa gatgatggtt aatttttacg acccaagtca
     1921 aggggagatt agtctgggtg gtgtcaatct caatcagatt gataaaaaag ccctgcgcca
     1981 gtacatcaac tatctgcctc aacagcccta tgtctttaac ggaacgattt tggagaatct
     2041 tcttttggga gccaaggagg ggacgacaca ggaagatatc ttacgggcgg tcgaattggc
     2101 agagattcga gaggatatcg agcgcatgcc actgaattat cagacagaat tgacttcgga
     2161 tggggcaggg atttcaggtg gtcaacgtca gagaatcgct ttggcgcgtg ctctcttgac
     2221 agatgcgccg gtcttgattt tggatgaggc gactagcagt ttggatattt tgacagagaa
     2281 gcggattgtc gataatctca ttgctttgga caagaccttg attttcattg ctcaccgctt
     2341 gactattgct gagcggacag agaaggtggt tgtcttggat cagggcaaga ttgtcgaaga
     2401 aggaaagcat gctgatttgc ttgcacaggg aggcttttac gcccatttgg tcaatagcta
     2461 gaaagaggag aggatgaaac cagaattttt agaaagtgcg gagttttata atcgtcgtta
     2521 ccataatttt tccagtagtg tgattgtacc catggccctt ctgctcgtgt ttttacttgg
     2581 ctttgcaact gttgcagaga aggagatgag tttgtccact agagctactg tcgaacccag
     2641 tcgtatcctt gcaaatatcc agtcaactag caacaatcgt attcttgtca atcatttgga
     2701 agaaaataag ctggttaaga agggggatct tttggttcaa taccaagaag gggcagaggg
     2761 tgtccaagcg gagtcctatg ccagtcagtt ggacatgcta aaggatcaaa aaaagcaatt
     2821 ggagtatctg caaaagagcc tgcaagaagg ggagaaccac tttccagagg aggataagtt
     2881 tggctaccaa gccacctttc gcgactacat cagtcaagca ggcagtctta gggctagtac
     2941 atcgcaacaa aatgagacca tcgcgtccca gaatgcagca gctagccaaa cccaagccga
     3001 aatcggcaac ctcatcagtc aaacagaggc taaaattcgc gattaccaga cagctaagtc
     3061 agctattgaa acaggtactt ccttggccgg tcagaatcta gcctactctc tttaccagtc
     3121 ctacaagtct cagggcgagg aaaatcccca aactaaggtt caggcagttg cacaggttga
     3181 agcacagatt tctcagttag aatctagtct tgctacttac cgtgtccagt atgcaggttc
     3241 aggtacccag caagcctatg cgtcagggtt aagcagtcaa ttggaatccc ttaaatccca
     3301 acacttggca aaggttggtc aggaattgac ccttctagcc cagaaaatct tggaggcaga
     3361 gtcaggtaag aaggtacagg gaaatctttt agacaagggg aaaattacgg caagtgagga
     3421 tggggtgctt catcttaatc ctgagaccag tgattctagc atggttgcag aaggtgccct
     3481 actagcccaa ctttatccat ctttggaaag agaagggaaa gccaaactca cagcctatct
     3541 aagttcaaaa gatgtagcaa gaatcaaggt cggtgattct gttcgctata ctacgactca
     3601 tgatgccggg aatcaacttt ttctagattc tactattaca agtattgatg cgacagctac
     3661 taagactgag aaagggaatt tctttaaaat cgaggcggag actaatctaa cttcggagca
     3721 ggctgaaaaa cttaggtacg gggtggaagg ccgtctacag atgattacgg gcaagaaaag
     3781 ttatctacgt tattatttgg atcaattttt gaacaaagag taatgttcgt gtttttagag
     3841 ttaaataatt tttaaactgt gagaaagatt cttcttgcag ttttttcttt acaatttttg
     3901 aaaaacatct gctatttatt cggttaaatt cttgtgtttt ttggtttttt gtggtaaaat
     3961 gtgctcaagt aatacgaaag gcgaacttta aaatgtcaaa acaattgatc tattcgggaa
     4021 aagctaaaga tatctataca actgaggatg aaaatcttat tatttcaact tacaaggacc
     4081 aggcgactgc tttcaatggt gtcaagaagg agcagattgc aggtaaggga gtcttgaata
     4141 atcagatttc atcttttatt tttgagaaat taaatgcggc tggtgtggcg actcactttg
     4201 tggagaaact ttcagacacg gaacaactca ataaaaaggt taagattatt cctttggaag
     4261 tcgtgctccg caactatact gctggttcct tttcaaaacg ttttggtgtg gatgagggaa
     4321 tcgccttgga gactccgatt gtcgaatttt actacaaaaa tgatgatttg gatgatccat
     4381 ttatcaatga tgagcatgtg aaattcctac agattgcgga tgaccagcag attgcctact
     4441 tgaaggaaga aacgcgtcgt atcaatgaac tattgaaagt ctggtttgct gagattgggc
     4501 ttaaattgat tgactttaag ctagagttcg gttttgacaa ggatggcaag attatcttgg
     4561 cagacgaatt ttcaccagat aactgccgct tgtgggatgc tgatggcaac cacatggata
     4621 aggatgtttt ccgtagagga ttgggagaac taaccgacgt ttatgagatt gtttgggaaa
     4681 agttgcaggg tttaaaataa caacctcaag gctgtttggg aatattgcaa gagctgaaat
     4741 aaaggaataa gaattgatgg ataaacgtat ttttgttgaa aaaaaggctg attttcaggt
     4801 caagtcagag agtttggtta gagagctcca gcacaacttg ggactgtcaa gcttgaaaag
     4861 tattcgtatt gtgcaagtat atgatgtatt tgacttggtt gaggacttgt ttgcacctgc
     4921 agagaagcac attttctctg agcaggtaac cgaccatgtt ttagatgaag tatctgtgca
     4981 ggcggatctt gctaactatg ctttctttgc cattgaaagt ctaccagggc agtttgacca
     5041 gcgtgcagct tcgtcacagg aagccttgct tttgttggga agttcgagtg acgtgacagt
     5101 caacacagcc caactttact tggtgaataa agatattgat gcgactgagt tggaagctgt
     5161 caaaaactac ctgctcaatc cagttgattc tcgtttcaag gatatcacga tagggattgc
     5221 caagcaggag ttttcagagt cagacaagac cattccaaaa ttgcctttct ttgaaagcta
     5281 tacagcagaa gactttgctc gctacaaggc cgaacaaggg atggccatgg aagtggatga
     5341 tttgctcttt atccaagact actttaagtc aatcgggcgc gtgccaactg agactgaact
     5401 caaggttttg gacacttact ggtctgacca ctgccgtcat acgacttttg agacagagtt
     5461 gaaacacatc gacttttcag cttctaaatt tcaaaagcaa ttgcagtcaa cctatgacaa
     5521 gtatattgcc atgcgcgagg aattaggtcg gtctgaaaaa ccacaaacct tgatggatat
     5581 ggcgactatt ttcggtcgtt atgagcgtgc taatggacga ttggatgata tggaagtctc
     5641 tgacgaaatc aatgcctgct cagttgaaat tgaagtggac gttgatggtg tcaaggaacc
     5701 ttggctcctc atgtttaaaa acgaaactca caaccatcca acagaaattg agccatttgg
     5761 tggagcggct acctgtatcg gtggagctat tcgtgatccg ttgtcaggcc gttcctatgt
     5821 ttaccaagcc atgcgtattt caggtgctgg tgatattaca gcaccgattt cggaaactcg
     5881 tgctgggaaa ttgccacaac aagtcatttc taaaacagca actcatggtt attcttcata
     5941 tggtaaccag attgggcttg ccacaaccta cgttcgtgaa tacttccacc caggctttgt
     6001 agctaaacgt atggaacttg gtgccgttgt tggtgcggct cccaagggca atgttgtccg
     6061 tgaaaaacct gaagcaggtg atgtgatcat ccttctcgga ggtaaaacag gtcgtgatgg
     6121 tgtcggtggt gcgacgggct cttctaaggt tcaaacagtt gagtctgtag agactgctgg
     6181 tgctgaggtt caaaaaggaa atgccatcga agaacgcaag attcagcgcc tcttccgtaa
     6241 tggcaatgtc actcgtctga tcaagaagtc caatgacttt ggggcaggcg gtgtctgtgt
     6301 ggctatcggt gaattggcag acggtcttga aatcgacctc aacaaggtgc ctcttaaata
     6361 ccagggcttg aatggtacag aaattgccat ctctgaatca caagaacgga tggcggtcgt
     6421 ggttcgtcct gaagatgtgg ataccttcgt tgccgaatgt aacaaagaaa atattgatgc
     6481 tgttgtggtg gcgacagtaa ctgaaaaacc aaatcttgtc atgcactgga atggtgagac
     6541 aatcgttgac ttggagcgtc gtttccttga catcaatggt gtgcgcgtgg ttgtcgatgc
     6601 caaagttgtg gacaaggatg tcaaactccc agaagagcgt caaacatctg ctgaaacact
     6661 ggaatcagat acccttacgg ttctatctga cctcaaccat gcaagtcaaa aaggattaca
     6721 gactatcttt gactgctctg ttggacgctc aacggttaat cacccacttg gtggtcgtta
     6781 ccaactcaca ccaactgagg catctgtgca gaaattgcca gttcaacacg gtgtgactca
     6841 tactgcgtcg gtcattgctc aaggtttcaa cccatatgta gctgaatggt ctccatacca
     6901 cggtgctgct tatgcggtta tcgaagcaac tgctcgtttg gtggctgttg gtgccaactg
     6961 gtccaaggct cgtttctctt accaagaata tttcgagcgt atggacaagc aagcagagcg
     7021 ttttggtcag ccagtagccg ctcttctagg ctctattgaa gctcagattc aacttggctt
     7081 gccatctatc ggtggtaagg actccatgtc tggtaccttt gaagaattga ccgttccgcc
     7141 aaccttggtt gcctttgggg tgacgacggc agatagccgt aaggtgctct ctccagaatt
     7201 taaagctgtt ggggaaaata tctactacat cccaggtcaa gccctctctg cagagattga
     7261 ttttgacttg attaagaaaa attttgctca gtttgaagca agccaagctg accataaagt
     7321 gacatctgca tcagctgtca aatacggtgg tgtagttgaa agtttggctc ttgctacctt
     7381 tggaaactat attggtgcag aggtgacctt gcctgaactt aaaacagctt tgacagctca
     7441 attaggcggc tttgtcttca catctcctga agaaattgct ggagtagaga agattggaca
     7501 aacgaaagca gactttacac tgactgtcaa cggtgtgaag ctagatggac acaagcttga
     7561 cagtgcattt caagggacat tggaagaagt ttacccaaca gaatttaccc aagcgaaaga
     7621 actagaagaa gtaccagctg tggcatcaga tgttgtgatt aaagccaaag aaaaggttga
     7681 aaaacctgtg gtttacatcc cagtctttcc aggaaccaac tcagaatatg attcagctaa
     7741 ggccttcgaa aaagaaggtg cagaggtcaa tttggtgcca ttcgtgacct tgaatgaaga
     7801 agctattgtc aagtcagttg aaactatggt tgacaatatc gacaagacta atattctctt
     7861 ctttgctggt ggattctcgg ctgcggatga accagatggt tcagctaagt ttatcgtcaa
     7921 tatcctgctt aatgaaaaag tgcgtgtggc tattgatagc tttatcgccc gtggtggttt
     7981 gattatcggt atttgtaatg gattccaagc cttagtcaaa tcgggtctcc taccctacgg
     8041 aaactttgaa gatgctaaca atactagccc aaccctcttc tacaatgatg ccaaccaaca
     8101 cgtggccaag atggtggaaa ctcgcattgc caataccaac tcaccatggt tggctggtgt
     8161 gcaagtgggc gatatccacg ctattcctgt ttcgcacggt gaagggaagt ttgtcgtgac
     8221 ggctgaggaa tttgcagagc tccgtgacaa tggacaaatt ttcagccaat acgttgactt
     8281 taacggtaaa ccaagtatgg attctaagta caatccgaat ggttctgtcc atgccatcga
     8341 aggaattacc agcaagaatg gtcaaatcat cggtaagatg ggccattcag aacgttatga
     8401 ggacggtctt ttccaaaata tcccaggcaa taaagaccag cacctgttcg cgtcagcggt
     8461 taaacatttc actggaaaat aagacttaca gattttctaa tagatagtat cagtaatgta
     8521 aaagtcatgt aaatctagct cttgatgatt acaaatgaaa attaggtata aaaaatgaca
     8581 tacgaagtaa aatctcttaa tgaagaatgt ggtgttttcg gtatttgggg acatccagat
     8641 gctgctaagt tgacctattt tggactccac agtcttcaac accgtggtca ggagggggca
     8701 ggaatcctct ccaatgatca aggacaactg aagcggcatc gcgatatggg tcttctatca
     8761 gaagtcttca gaaatcctgc taacttagat aaattgacgg gaactggtgc gattgggcat
     8821 gtgcgttacg cgactgctgg cgaagcttct gtagataata tccagccctt cctcttccgt
     8881 tttcacgata tgcagtttgg cttggctcat aatggaaatc tgaccaatgc agcctctctc
     8941 aagaaagaac tggaacaaag aggagcaatt ttcagcgcga cttcggactc tgaaatcttg
     9001 gctcacctca ttcgtcgcag tcataatcct agcctgatgg gcaaaatcaa ggaagcgctc
     9061 agccttgtca aaggtggttt tgcctatatc ttgctgtttg aggacaagtt gattgcggct
     9121 cttgacccaa atggattccg accgctttcg attggtaaaa tggctaatgg agcagttgtt
     9181 gtatcttctg aaacctgtgc ttttgaggtc attggtgccg agtggattcg tgatttgaag
     9241 ccaggtgaga ttgtgatcat tgatgacgag ggcattcagt atgacagcta tacagatgat
     9301 acccagttgg cggtttgttc tatggagtat atctactttg ctcgccctga ttctaatatc
     9361 cacggtgtca atgtccatac ggcacgtaag agaatgggag cgcaattggc gcgagaattt
     9421 aagcatgagg cagatattgt agttggtgtg cccaattctt ccctaagcgc ggctatggga
     9481 tttgcggaag aatcaggctt accaaatgaa atgggtctga tcaaaaacca atacacccag
     9541 cgaactttta tccaaccgac tcaagaattg cgggagcaag gagtgcggat gaaactgtct
     9601 gctgtttcgg gtgttgtcaa aggcaaacgt gtggtcatgg tggatgattc cattgtacgt
     9661 ggaacaacct ctcgtcgtat cgttcagctc ttgaaagaag cgggtgcgac tgaggttcac
     9721 gttgccattg gaagtcctgc actagcgtat ccatgtttct acgggattga tatccagacc
     9781 cgtcaggagc tgattgcagc caatcatacg gtcgaagaaa ctcgccaaat cattggtgcg
     9841 gacagtatga cttatctttc aattgatggc ttgattgagt cgattggtat cgaaacagat
     9901 gcgccgaacg gtggtctctg tgtcgcttac tttgacggtg actacccaac gcctctctat
     9961 gactacgaag aagactatcg tagaagtttg gaagaaaaga ccagttttta caagtaggcg
    10021 acagattctc cattaaagaa aaggaaaaaa caaatgacaa ataaaaatgc atatgcccag
    10081 tcgggtgtgg atgttgaagc gggttatgaa gttgttgaac ggattaaaaa gcacgtggcc
    10141 cgtacggagc gtgcaggtgt catgggagct cttggtggct ttggtggtat gtttgacctt
    10201 tccaagactg gggttaaaga acccgtcttg atttcaggga ctgacggtgt cggaaccaag
    10261 ctcatgttgg ctatcaagta cgacaagcac gataccatcg ggcaggactg tgtggccatg
    10321 tgtgtcaacg acatcattgc tgcaggtgcg gaacccctct attttctcga ctacgtagcg
    10381 acagggaaga atgaaccagc taagctagaa caagtggttg ctggtgtggc agaaggttgt
    10441 gtgcaggctg gtgctgccct catcggtggg gaaacggctg aaatgccggg catgtacggc
    10501 gaagacgact atgacttggc tggttttgcg gtcggtgtgg ctgaaaaatc tcaaatcatt
    10561 gacggttcaa aggtggtaga gggagatgtt cttctcggac ttgtttcaag tgggattcac
    10621 tcaaatggtt actctttggt tcgtcgtgtc tttgcggatt acacaggtga ggaagtccta
    10681 ccagaattgg aaggcaagaa acttaaggaa gttctacttg agccgactcg tatctatgtc
    10741 aaggctgtct tgccgctcat caaagaagag ttggtcaacg gcattgccca catcacaggt
    10801 ggtggcttta tcgaaaatgt ccctcgtatg tttgcagatg acctagctgc tgaaattgat
    10861 gaaagcaaag ttccagtgct tccaattttc aaagcccttg aaaaatatgg tcagattaaa
    10921 cacgaagaaa tgtttgaaat cttcaatatg ggtgtgggac ttatgttggc ggtcagccct
    10981 gaaaatgtag agcgtgtaaa agaattgttg gatgaagcag tctatgaaat tggtcgcatc
    11041 gtcaagaaag aaaacgaaag tgtcattatc aaatgaaaaa aatagcggtt tttgcctctg
    11101 gtaatggctc aaattttcag gtgattgccg aagaatttcc agtggagttt gtcttttcag
    11161 accatcgtga tgcctatgtg cttgagcgtg caaagcagct cggcgttctg tcctatgctt
    11221 ttgaactcaa ggagtttgag agcaagacag actacgaagc agcccttgtc gaactcttgg
    11281 aagaacacca gattgacttg gtttgcctag caggctacat gaaaatcgtt ggaccaacct
    11341 tattgtcggc ttatgaaggt cggattgtca acattcatcc agcctatttg ccagaatttc
    11401 caggagctca tgggattgag gatgcttgga atgctggcgt gggtcagtct ggtgtgacca
    11461 ttcactgggt ggattcgggt gtggatacag gccaggtcat caaacaggtt cgtgtgccac
    11521 gactagctga tgataccatt gacagatttg aagctcgcat ccatgaagca gagtacaggt
    11581 tgtatccgga agtagtgaag gctctattta cagattgact ttttgatgat tcatatgata
    11641 tctttgattt taaattggag tcagtgtttg ttgaagacgg cttcaaacgg aggtatttgt
    11701 aatgttagaa tctaaaaaaa caactcgata tgtattttat gtctatctga tgttattaac
    11761 ttggggaatc ttatttaagt ttgaaacaaa tcctgaattt atagcatttt tcttagctcc
    11821 aaggtatatc aattggattc cattttcaga accactaata gtcgatggaa aaattgtttt
    11881 tgctgaaatg ttatttaatc tgatttcctt tattccatta ggtgtttgtt tccctttgat
    11941 aaaaactaat ttatctagtt taagaatagt cgggacaggt ttcttgatta gtttattgtt
    12001 tgagtgctta cagtatattt tagcaatagg tataacagat ataacggatt tgactttaaa
    12061 tacgctaggt gtctgtgtag gcttactgat ttatcaaatt tttataagag tgttcaaatc
    12121 acagactaga aaatggatca atatcttagg tatgcttagc cttggttttg cttatcttgt
    12181 tttactgtta ctgcatttaa ttggtgttta actaatgatt aaaaaggaga atataatgac
    12241 taaacgcgtc ttaatcagcg tctcagacaa agcgggcatt gttgaatttg cccaagaact
    12301 aaaaaaactt ggttgggaga ttatctcgac aggtggaact aaggttgccc ttgataatgc
    12361 tggggtggag accattgcga tcgatgatgt gacgggcttc ccagagatga tggatggccg
    12421 tgttaagact cttcatccaa atatccacgg gggtcttctc gctcgtcgtg atttggatag
    12481 ccacttggaa gcagccaatg agaaccaaat tgagcttatt gaccttgtag tggtcaacct
    12541 ttatcctttc aaggaaacga ttctcaaacc agacgtgacg tacgctgatg ctgtggagaa
    12601 tattgatatt ggtgggccat ctatgcttcg ttcagcagcg aagaatcatg ccagcgtgac
    12661 ggttgtggta gaccctgctg actatacagt ggttttggac gagttgtcag ccaatggtga
    12721 aacgacttac gaaacgcgtc aacgtttggc agccaaggtt ttccgtcaca cagcggctta
    12781 tgacgctttg attgcagaat acttcacagc tcaagtgggc gaaagcaaac ctgaaaagtt
    12841 gaccttgacc tatgacctaa aacaagctat gcgttatggg gaaaatcccc agcaggatgc
    12901 ggacttctac cagaaagctt tgccgactga ttactccatt gcatcagcca aacagcttaa
    12961 cggtaaggaa ttgtcattca acaatatccg cgatgcagat gctgccattc gtattatccg
    13021 tgacttcaaa gaccgtccaa ccgttgtggc tctcaagcat atgaatccat gtggtatcgg
    13081 ccaggcggat gacatcgaga cggcttggga ttatgcttat gagtctgacc cagtgtctat
    13141 ctttggtggt atcgctgttc ttaaccgtga ggtggatgct gcgacagctg agaagatgca
    13201 cggcgttttc ctcgaaatca tcattgcacc aagctatacg gatgaagcgc tagccatttt
    13261 gatcaataaa aagaaaaact tgcgtatcct tgccttgcca tttaatgctc aagaggctag
    13321 cgaagtggaa gcagaataca caggtgtagt cggtggactt ctcgtgcaaa atcaagacgt
    13381 ggtcaaggaa agcccagctg actggcaagt ggtgaccaaa cgtcagccaa ccgagacaga
    13441 agcgactgct cttgagttcg cttggaaggc tatcaagtac gtcaaatcaa atggtattat
    13501 cgtgaccaac gaccacatga cacttggtgt tggtccaggt caaaccaacc gtgtggcttc
    13561 tgttcgcctt gccattgacc aagccaaaga tcgtctggac ggggcggtcc ttgcttcaga
    13621 tgccttcttc ccatttgcgg ataacgtgga agaaatcgcc aaagcaggaa ttaaggccat
    13681 catccagccc ggtggctctg tccgtgacca agaatccatc gaagcagcgg ataaatacga
    13741 cttgactatg gtctttacag gtgtgagaca ttttagacat taagaagata aaagggaaga
    13801 aaacagtttc tttccttttt tggcttaaaa tgctaactga aacaagatta aaacgaactt
    13861 ttttgatata atgttggtaa ataattcgca aaagaggttg aggaatgaaa ctacttgttg
    13921 tcggttctgg tggtcgtgag catgcgattg ctaaaaagtt acttgaatca aaagacgtgg
    13981 aaaaagtctt tgtagctcct gggaatgatg ggatgactct ggatggtttg gaattggtaa
    14041 atatctctat ttccgaacat tctaaattga ttgacttcgc aaagaccaat gatgttgctt
    14101 ggacctttat cggtccagat gatgcccttg ctgctggtat cgtggatgat tttaaccaag
    14161 ctggacttaa ggcctttggt ccgactaggg ctgcagcgga gctggagtgg tccaaggatt
    14221 tcgccaagga aatcatggtc aaatacggcg ttccgacagc aacatatggc acattttcag
    14281 atttcgagga agccaaggcc tatatcgaaa atcatggtgc gcctatcgta gtcaaggcgg
    14341 atggcttggc acttgggaag ggtgtcgtcg ttgctgagac ggttgagcaa gcggtcgaag
    14401 ccgctcatga gatgcttttg gacaataaat ttggtgactc aggtgcgcgc gtggttattg
    14461 aggaattcct tgaaggagag gaattttcac tctttgcctt tgtaaatggt gataagttct
    14521 acatcatgcc aatggctcag gaccacaaac gtgcctatga tggcgacaaa gggcctaaca
    14581 cgggtggtat gggtgcctat gcgccagtcc cacacttacc acagagtgta gttgatacag
    14641 cggttgacac cattgtcaag ccagttctag aaggggtgat taaagaaggt cgcccttatc
    14701 tgggagttct ttacgcaggg cttatcctga cagctgatgg accgaaagtc attgagttca
    14761 acgctcggtt cggagatcca gaaactcaga ttatcttgcc tcgcttgacc tctgactttg
    14821 ctcaaaatat cacagatatc ctggatagca aggagccaaa tatcatgtgg acggacaagg
    14881 gtgtgactct gggtgtggtt gtcgcatcca agggctaccc gctagactat gaaaggggcg
    14941 ttgagttgcc agcaaagaca gaaggcgatg tcatcaccta ctatgcaggg gctaagtttg
    15001 cggaaaatag cagagcactg ctctcaaacg gcggacgagt ttatatgctc gttaccacag
    15061 cagataccgt caaagaagcc caagccagca tctaccaaga actatcccaa caaaaaataa
    15121 aaggactctt ctaccgaaca gatatcggaa gcaaggcaat taagtaaaga tataagaata
    15181 acgcgccgta gtcgccaaac acgataatgg tcgtcgtggt gaaaagacca gaacagtgaa
    15241 tgttctggtc agggggaaac ttggagacct taggctcaaa gtttaggaat gaaaccgaag
    15301 gtttgcttcc gcctccatca cctaagacca ttatcaaaaa gaaaaataaa aattcacaaa
    15361 atacgttaat gatcgtatgg tttgcgagcg ttagcgagct aatatagaac aatcaccgcc
    15421 gttgtgaaag aacgattgga tgataatcca atcgttcagg gaaattggaa gaccttgggt
    15481 ttccaattta ggcatgagac acctttggtg gctgctgccg tccctcacaa gctaaggtga
    15541 ttgttgaaaa agaggaaaaa ggagaagaaa tgaaaccagt aatttccatc atcatgggct
    15601 caaaatccga ctgggcaacc atgcaaaaaa cagcagaagt cctagaccgc ttcggtgtag
    15661 cctacgaaaa gaaagttatt tccgcacacc gtacaccaga cctcatgttc aaacatgcag
    15721 aagaagcccg tagtcgtggc atcaagatca tcatcgcagg tgctggtggc gcagcgcatt
    15781 tgccaggcat ggtagctgcc aaaacaaccc ttccagtcat tggtgtgcca gtcaagtctc
    15841 gtgctcttag tggagtggat tcactctatt ctatcgttca gatgccgggt ggggtgcctg
    15901 ttgcgaccat ggctatcggt gaagctggag cgactaacgc agctctcttt gccctccgtc
    15961 tcctctctgt agaagatagg tccattgcgg atgcacttgc caactttgct gaagaacaag
    16021 gaaaaatcgc agaggagtcg tcaaatgagc tcatctaaaa caatcggaat tatcggtggc
    16081 ggtcaactgg gtcagatgat ggccatttct gctatctaca tgggccacaa ggttatcgcg
    16141 ctggatcctg cggcggattg cccggcctct cgcgtggcgg aaatcattgt ggcaccttat
    16201 aacgatgtgg acgccctccg tcagttggca gaccgttgcg atgtcctcac ttacgagttt
    16261 gaaaatgtcg acgctgacgg tttggatgcc gttatcaagg atggacaact ccctcaagga
    16321 acagatctgc tccgcatttc gcaaaatcgt atttttgaaa aggacttttt gtcaaacaag
    16381 gctcaagtca ctgtggcacc ctacaaggtc gtgacttcta gcctagactt ggcagatatc
    16441 gacttgtcga aaaactatgt cctcaagact gcgactggtg gctacgatgg tcatggacaa
    16501 aaggttattc gttcagaagc agacttggaa gcagcctatg cgctagcaga ctcagcagac
    16561 tgcgtcttgg aagaatttgt caactttgac cttgagattt ctgtcatcgt gtcaggaaat
    16621 ggcaaggagg tgacgttttt cccagttcag gaaaatatcc accgcaacaa tatcctgtct
    16681 aagaccatcg taccagcccg catttctgaa agtctagcag acaaggctaa agctatggca
    16741 gtgcgaatcg cagaacaact caacttgtct ggaactctct gtgtggaaat gtttgcgact
    16801 gatgatgacg ttattgtcaa tgaaatcgcc ccacgaccac ataactctgg gcactattct
    16861 attgaagcct gtgatttctc tcagtttgac acccatattc tgggtgttct cggagcacca
    16921 ttaccagtca tcaaactcca tgcgccagcc gttatgctta atgtcctcgg tcagcatgtc
    16981 gaggctgctg agcaatatgt gacagaaaat tcaagcgccc acctccacat gtatggtaaa
    17041 atagaagcga agcacaaccg caagatggga catgtgacct tgtttagtga tgtaccggat
    17101 agtgtggatg agcttgggga agggattgat ttttaggaca agtctatgat acaaattatc
    17161 gttaatacat ttattgaaaa ggataagact ggagcagttg ttgaagtgtt gtatgccagt
    17221 gctgaccaag ataaggtaca agctaaatat gaagaactag ctgcacaata ccccgaaaat
    17281 tatttagcta tctatgatgt accgctggat acggatttga atacactaga tcattacccg
    17341 tctgtgttta ttgaaaaaga ggagtttgag tagaaatctt ggtttaccta gatagcttat
    17401 tcccaacagc ttaagaagaa aggaaaaatt aacacatgat caaccgttac tctcgccctg
    17461 agatggcgaa tatttggagt gaagaaaata aataccgtgc ttggcttgag gtggaaatcc
    17521 tctctgacga ggcatgggct gagttggggg aaatccctaa ggaagatgtg gctttgattc
    17581 gcaagaaggc ggactttgac atcgaccgta ttttggaaat tgagcaggag acgcgccacg
    17641 atgtggtggc tttcacgcgt gcggtttctg agactcttgg tgaagagcgc aagtgggttc
    17701 actatgggtt aacttctacc gacgtggtgg atactgctta tggttacctc tacaagcagg
    17761 ccaacgacat catccgtcgt gaccttgaaa acttcactaa tatcatcgct gacaaggcca
    17821 aggagcacaa gttcaccatc atgatggggc gtactcatgg tgtgcacgct gagccgacaa
    17881 cctttggtct taaattggca acttggtaca gcgaaatgaa acgcaatatc gagcgtttcg
    17941 agcatgcagc tgctggtgta gaagctggta agatttctgg tgcggttggg aactttgcca
    18001 atataccacc atttgtagag gagtatgtct gcgataaact tggcatccgt gcccaagaaa
    18061 tctctacaca agtccttcct cgtgaccttc acgctgagta ctttgcggtt cttgccagca
    18121 ttgcgacttc aatcgaacgt atggcgactg agattcgtgg tctacaaaaa tctgagcaac
    18181 gcgaagtaga agagttcttt gctaaagggc aaaaagggtc ttcagcaatg cctcacaaac
    18241 gcaacccaat cggttctgaa aatatgactg gtctggcgcg tgtcattcgt ggtcacatga
    18301 ttacggctta tgaaaacgtc gctctctggc acgaacgcga tatttctcac tcatcagctg
    18361 agcgtatcat cacaccagat acgaccattt tgattgacta catgctcaac cgttttggaa
    18421 atatcgtcaa gaacttgaca gtcttcccag aaaatatgat ccgaaacatg aactcgactt
    18481 ttggtcttat ctttagccaa cgggctatgt tgacattgat tgaaaaaggc atgacccgtg
    18541 agcaagccta tgacttggtg caaccaaaaa cagcctactc ttgggacaac caagtagact
    18601 ttaaaccact tcttgaggca gattcagaag taacatcacg tctcactcaa gaagaaatcg
    18661 atgaaatctt caacccagtt tattacacca aacgagtgga tgatatcttt gaacgtcttg
    18721 gactaggtga ttaattaaaa aataaacagc gagcttcaat ctcgctgttt attttttatc
    18781 gaaaagactt agtcttcttt tcttttagtg agtccatagg ctgctagtgt ggacatgagt
    18841 cctgcgacta ctagtcctgc agaatcgtga gttcctgttt caggaagttt tttctctgtt
    18901 accacaggag ctggatcttg aggaagagct ttgctttcct cagcaggagc agttgatgga
    18961 gctggttgtc ttgggatttc tagttttggt ttttcttcag caatagcggc ttgtccgttt
    19021 tcatcgccta catgtgttac catagttccg acttcgacta tttgagtaac ggcttcctgt
    19081 gctacgacac tatttacaag tgttttcact tccttaccat cggcagaagt gctcacagag
    19141 tagaagttgc tacgatgtcc attgacgccc ttagtaatga cttgtgtttt tcctttgagt
    19201 aagagtggat tttcacgagt cactgtggta aatggaattt cttctttttg gatatccagt
    19261 ctaggtttta cctcagtagt tggtgcaaga ccactttcat cacccttgtg agttacagga
    19321 gcgccaactt caaccacttg gtttataact tctttggtta cctggctatc aaggactgtt
    19381 tctgttgttt ttccattttc agtgagtaca gagatgtaat gagttcgttc acctttgact
    19441 cctgctgtga taatattttc ctgaccagct gggaggttag gattttcttt cttgataact
    19501 tcaaatggaa tttcttcagt tcttgtgatg agttctggtc tggtttcaac attggcagcc
    19561 acttcatttt catctaggct tcctgaatga gttgcagctg gtttgaggcc ttgaagagcg
    19621 gcttttaggt tggctacaag tgtgtcaagc tcagcttgtt tattacggtt gaggttgtaa
    19681 tttagagctg ttttagctgc gtcaagggcc tcaagacttt ctttactata tccttctaag
    19741 tttgtaggaa ttttagctaa ttcttcgcgg agagcattat aattagcacg gaagtagtct
    19801 ttgttgtggt ctgcaaaggc agtcatgagt tcaaagattt cctcttcctt gtattcagcg
    19861 cttggtctat ctgcccagat tgaaagcata cttccgactg ttggaagatc tacttcagga
    19921 tatttggtag aagctagttg attgaatggt gtttttccag tattctcaat agctttcttg
    19981 aggaaaccac caccatcttc tggtttttga ccaagaatgt agtaccagtc accgttggta
    20041 ttcaagaatt tatagccttt gcttgctagg tattgaggtg atgcgaggtt atatccccac
    20101 cagcctttag accagtaaga aatcaagaca tctttgtcaa actgaacatc gtccttgtct
    20161 tcatagtaga agccatcgtt gaaggccatt ggttgaagcc ctctttcttt ggccatagct
    20221 gcgagggtgt tggcatattc ggcaaatttg ccatagagtt gataccactt gaggtagtac
    20281 cagccttggg cactagtcgc atcgttggcg tattcgtcag taccaaagtt gaaaatcttt
    20341 gttttacctg caaagaagtc catgtattta ccgatgaggg cttttacaaa gttcatcgct
    20401 tcttcgtttt tcaagtccat agttgttttt gaaactttat caaagtgggc ttgaggattt
    20461 ttaataccta atttttccat ggcaaccagc atagcatcca tgtgacctgg actgttaata
    20521 gctgggatga gaccaatgtc cttagattta gcgtattcaa ttagctctgt cacttctgcc
    20581 tgtgttagtg cagtaccgtt tggatcgtcg tagtaagctt tagttccttc gataatagct
    20641 tttttaacgt catcactagc ataggttttt ccgttggcag taatggtcat atcattgagt
    20701 agaaagcgaa gtccgtcatt tcctagaagg agatggacat cagaatatcc gagctcactg
    20761 gccttgtcta cgatgcgttt gagctggtcc agagtaaagt atttgcgtcc agcatcgatt
    20821 gagattacct tgtttttggc aagtttttca acctcacgtt tagcttcttc ttctttttga
    20881 gcttcaggcg tgagggtcaa gttgttgaca gtttcttgaa gtttagcaat ggcttgatca
    20941 atcgtatctt gttgggcacg gctaaggttg ctatcgagag agcgaatagc tttttcagct
    21001 tcttttacgg ccgcgacgct ttctgcagta taacggttca ggtcttttgg tacctcgtta
    21061 agtgcttgct ctgcagattc ataatcagct gcgaagtatt cagcgttggc atttgcaaaa
    21121 tgacgcatga gtttgaagag gtgtgatggt gaataacgtg cagatggagt gtcagcccaa
    21181 gcagctacca taccaccgat gattgggata tcagctcctt ctgtttttgg tacagaagtg
    21241 attggtgtgt ttttaatacc attgagcccc tgatcgagat tgtaccagcc ttggccatca
    21301 gcgtttcgtc caagaacgta gtaccaagca tcattggtat taaggatttg gtgacctttt
    21361 tcagctagta gtttagaaga agcgacatcg tagcctcccc aaccaccagt ccacatagaa
    21421 acgatgatgt ctttgtcaaa actaccaaag cttgtgtcgc tattgtagta gataccgtcg
    21481 ttaaaggcca ttggtttgag accgtgcgat tttacaatac gagcgaggtc attggcgtag
    21541 gcaataaatt tttcatagcc ttttacaggg tagccttcgt ttggatagta tttatcagct
    21601 tgaagcacac tccaaccttt agcatctgtc gcatcattgg catattcatc aagtccgatg
    21661 ttgaagattt cagtcttttt cgcgaaataa gcagcatact tgtcgataag ggcttttgta
    21721 aaagcgacag cttgttcgtt gtcaagatcg acagtacggg ctgattcctt cccaaaatag
    21781 ctaaagttag ggttttggat tcccaattct ttcatggcat tgagaatcgc atccatgtga
    21841 ccaggactat ttactgtcgg aatgagaccg atacctttat cttttgcata gttaatcaga
    21901 tctgtcattt gactttctgt taagtgattg ccgtttggat cgttgtaata atcatttgta
    21961 cctttttcaa tggcgcgttt gacatcgtca ctggcatagg tcttgccgtt agctgtgatg
    22021 ctcatatcgt ccaacatgaa acggagtcca tcatttccga ctaataggtg taaatcagtg
    22081 tagccataat gtttcgcttt atcgatgatt tccttgagct gttctggtga gaaatattta
    22141 cgtccagcat caatagaaac aattttcttt ttcgctagtt tttcacttac agttgcagca
    22201 cgttcctttc ctgcctctgt tgccggtttg tcagcctctg ctttcgcttc atctttttta
    22261 gctggtttat cctttttagg gctagcttct tctgcctttt tattagcagt ttctttttca
    22321 gcagaagttg gagttaccac ttctgcttta tcactaggag ttgaactaac ttcctcttgt
    22381 ggtttttctt ctgtttttgg aagactagct accttatcag tagctggagt ttctgtttct
    22441 acagtttttg gagcttctgg ttgaagctct gctttaggtg tttcctcagt ccgattttcg
    22501 gatgattgag gggaatcaga aaccgtatgg atggtcggtt ggttttctgt agtagtagga
    22561 gtaactccat cggctgcaac agtctgtgct tggaaggcaa atccaattag aacagaagct
    22621 gctcctacag cgtatttacg aatagaaaaa cgctgttgtt tttcatgttt cattgcaaaa
    22681 cctcctgatt gcattgttat attgatagcg attatataaa tcaacgcctt tattttattt
    22741 cttgttttaa cgagagtcaa gagg
//
LOCUS       DBERDK010000028        22136 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.028, whole genome shotgun sequence.
ACCESSION   DBERDK010000028 DBERDK010000000
VERSION     DBERDK010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 22136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22136
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.028"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            70..981
                     /locus_tag="ACL73L_001090"
     CDS             70..981
                     /locus_tag="ACL73L_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGS0772085.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSLVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCVEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPVYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVAVISASEPLSSALLSVLFLGLVV
                     DWSLLLAIALIILPMIFLSIEEAKESR"
     gene            1211..1909
                     /gene="dapD"
                     /locus_tag="ACL73L_001091"
     CDS             1211..1909
                     /gene="dapD"
                     /locus_tag="ACL73L_001091"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HGS0772086.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLG
                     NVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVEI
                     GDNAVIMMGAVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVRVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEDALRTL"
     gene            1977..3107
                     /locus_tag="ACL73L_001092"
     CDS             1977..3107
                     /locus_tag="ACL73L_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HGS0772087.1"
                     /translation="MLDLIQTRRALHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLPFASQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEDGAFGDWLPNQFYGLHVRPDLK
                     VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVN
                     PIEGAVVTFGVFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFD
                     MEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE"
     gene            3119..3658
                     /locus_tag="ACL73L_001093"
     CDS             3119..3658
                     /locus_tag="ACL73L_001093"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HGS0772088.1"
                     /translation="MKSELRKQVLHEMKALSQEQKQAIDQALTERILQHPFYQEAKVI
                     ATYLSFSHEFQTRELIEQALKDGKKVLIPKTYPKGRMDFVVYDPQQLVKTAFALLEPQ
                     GDLEVVDVSQIDLIHVPGLAFTTDGYRIGYGGGYYDRYLEHFSGHTLSTVHPCQIQDF
                     IPENHDIPVQEVLIDEGNL"
     gene            3642..4319
                     /locus_tag="ACL73L_001094"
     CDS             3642..4319
                     /locus_tag="ACL73L_001094"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HGS0772089.1"
                     /translation="MKEIFDRRYPVTSFFLLVTALVFLLMLVTAGGNFDRADTLFRFG
                     AMYGPAIRLFPEQVWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIVLRYATRNPYIQQLGQSYLTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRMYNTSQRLGAVVLFVGLAILL
                     FYKGMGM"
     gene            complement(4391..5290)
                     /gene="galU"
                     /locus_tag="ACL73L_001095"
     CDS             complement(4391..5290)
                     /gene="galU"
                     /locus_tag="ACL73L_001095"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HGS0772090.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKTDLLKLVDKTTDMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKAVPLTKQLMDDYERTHAST
                     IAVMPVPHDEVSAYGVIAPQGEGKDGLYSVETFVEKPAPEDAPSDLAIIGRYLLTPEI
                     FQILENQAPGAGNEIQLTDAIDTLNKTQRVFAREFNGARYDVGDKFGFMKTSIDYALK
                     HPQVKDDLKNYLIQLGKELTEKE"
     gene            complement(5312..6328)
                     /locus_tag="ACL73L_001096"
     CDS             complement(5312..6328)
                     /locus_tag="ACL73L_001096"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HGS0772091.1"
                     /translation="MKKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     HHTNKHYFKDVVLDENIIAYTDLAETLKDVDAILFVVPTKVTRLVAQQVAQTLDHKVI
                     IMHASKGLEPDSHKRLSIILEEEIPEHLRSDIVVVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQELFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGASPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRAAYELAQELGVYMPITQAIYQVIYHETNIKDTIYDIMNNEFKAE
                     NEWS"
     gene            6540..6980
                     /locus_tag="ACL73L_001097"
     CDS             6540..6980
                     /locus_tag="ACL73L_001097"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGS0772092.1"
                     /translation="MREFGEKIKRLRLAKKISRSEFCGDESELSIRQLIRIENGESRP
                     TLTKLKYIAERLGVEDYKLMPSYIELDKEYLELKYFLMRTPTYEDETIVQKKESVFAK
                     IFEEYYDRLPEEERFIIPNYSYLALTNYTVQKLPEKLVEILSFW"
     gene            complement(7095..7526)
                     /locus_tag="ACL73L_001098"
                     /pseudo
     CDS             complement(7095..7526)
                     /locus_tag="ACL73L_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7588..8238)
                     /gene="phoU"
                     /locus_tag="ACL73L_001099"
     CDS             complement(7588..8238)
                     /gene="phoU"
                     /locus_tag="ACL73L_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HGS0772093.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKALLALASKDKEMAEL
                     IINKDHAINQGQSAIELTCARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPDEEQLHQMGKLSLSMLADLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
     gene            complement(8253..9005)
                     /gene="pstB"
                     /locus_tag="ACL73L_001100"
     CDS             complement(8253..9005)
                     /gene="pstB"
                     /locus_tag="ACL73L_001100"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HGS0772094.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWEEVKDDLKKSAMSLSGGQQQRLCIARA
                     LAVEPDILLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            complement(9007..9822)
                     /gene="pstA"
                     /locus_tag="ACL73L_001101"
     CDS             complement(9007..9822)
                     /gene="pstA"
                     /locus_tag="ACL73L_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HGS0772095.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLIIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDSMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            complement(9815..10678)
                     /gene="pstC"
                     /locus_tag="ACL73L_001102"
     CDS             complement(9815..10678)
                     /gene="pstC"
                     /locus_tag="ACL73L_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HGS0772096.1"
                     /translation="MIKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFMANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     VYGFLKSAINLMAAIPSIVYGFFGLQLLVPWIRSFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNQPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            complement(10796..11671)
                     /locus_tag="ACL73L_001103"
     CDS             complement(10796..11671)
                     /locus_tag="ACL73L_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HGS0772097.1"
                     /translation="MKFKKMLTLAAIGLSGFGLVACGNQSAASKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGEYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            complement(11838..13169)
                     /gene="pnpS"
                     /locus_tag="ACL73L_001104"
     CDS             complement(11838..13169)
                     /gene="pnpS"
                     /locus_tag="ACL73L_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system histidine kinase PnpS"
                     /protein_id="HGS0772098.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMALTWISKGIGLFLLALSLGLGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSGLKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            complement(13170..13877)
                     /locus_tag="ACL73L_001105"
     CDS             complement(13170..13877)
                     /locus_tag="ACL73L_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGS0772099.1"
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGIEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGEQEGDDSDNIADDSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKA
                     PQFIRTIRGYGYKFKEL"
     gene            complement(13961..14446)
                     /locus_tag="ACL73L_001106"
     CDS             complement(13961..14446)
                     /locus_tag="ACL73L_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HGS0772100.1"
                     /translation="MAPTDKEDLIRTLELAPHVEGGYFRQTKKASDTINLAGKGRALY
                     TSIYFLLEETNPSHFHRLTADEIWYFHAGSPLTVHMITADGHYEAVTLGLDISKGQQL
                     HYCVPKGTIWGSTVDKDYALVSCLVAPGFEFEDFELFERVDLLATYPEHKEMIERLTR
                     Y"
     gene            14432..14989
                     /locus_tag="ACL73L_001107"
                     /pseudo
     CDS             14432..14989
                     /locus_tag="ACL73L_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(15304..16995)
                     /gene="argS"
                     /locus_tag="ACL73L_001108"
     CDS             complement(15304..16995)
                     /gene="argS"
                     /locus_tag="ACL73L_001108"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HGS0772101.1"
                     /translation="MNIKELIASELSSIIDSLDQEAVLKLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIAAELAEKMNSQAFEKVVATGPYVNFFLDKSAISAQVLQAVTTEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGDEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NGDEEALALWQWFRDESLVEFNRLYNELKVEFDSYNGEAFYNDKMDAVVDILSEKGLL
                     LESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTVAEAVSR
                     AKVQIEAKNPELENKDQVAHAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPETAGNYSLNDTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQSFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
     gene            17131..17577
                     /gene="argR"
                     /locus_tag="ACL73L_001109"
     CDS             17131..17577
                     /gene="argR"
                     /locus_tag="ACL73L_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HGS0772102.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRD
                     LREIGLTKVKKNDMVYYVLVNETEKIDLVEFLSHHLEGVARAEFTLVLHTKLGEASVL
                     ANIVDVNKDEWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK"
     gene            17628..20162
                     /gene="mutS"
                     /locus_tag="ACL73L_001110"
     CDS             17628..20162
                     /gene="mutS"
                     /locus_tag="ACL73L_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HGS0772103.1"
                     /translation="MAIEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSSKPDSQNNFLVSIDCEGNQFGLAYMDLVTGDFYVTGLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSRQMNLVLSYEKESFEDLHLLDLRLATVEQT
                     ASSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIVQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPTLAYLIAQLDAIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRCVLREGTSWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREKSA
                     NLEYEIFMRIREEVGKYIQRLQALAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQID
                     IRKGRHAVVEKVMGAQTYIPNTIQMAEDTSIQLVTGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTESPPPMRQT
                     SAVTEQISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL"
     gene            20309..22003
                     /gene="patA"
                     /locus_tag="ACL73L_001111"
     CDS             20309..22003
                     /gene="patA"
                     /locus_tag="ACL73L_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatA"
                     /protein_id="HGS0772104.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLDALLTG
                     KYEAIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVRMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGMMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTEV
                     SDELFGQNLYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMVQSDPSVVGSIASFVNYL
                     SQIIFTIVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDIPDEELVGSLSFENVTF
                     TYPMDKEPMLKDVSFTIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQNGAIKIGGKDI
                     REVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANIAQASEFIHRM
                     EKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAKSERLVQEALN
                     KDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVYREIYETQK"
BASE COUNT         6280 a         4383 c         4838 g         6635 t
ORIGIN      
        1 gataattctc taaatgaagt ggccaatcaa tggattgcca cttttttcct ttcgtgttac
       61 aataagggta tgaatcagta tcagaaaaag attgttaacg gaaaaattta ttcgctccta
      121 tccggcttaa tatggggaat ctgtggaatt ttaggagagt acttctttac tcattatcag
      181 gtgtcttcgg gctggattac ctctatgcgt ttgacactgg cagggagtct tgtactcatt
      241 tggtctgcaa tacaattaaa atcgcaagtg ctagatattt ggcgagacaa gaaaaattac
      301 ctgccctttt tagcttatgc tattttgggg attttttcag tccagtattt tttctatctc
      361 tgtgtagaat actcaaatgc tacgacagca actattttac agtttattag ccctgtcttt
      421 atcctctttt acaatcgctt ggtttatcaa aaacgagcgt caaaaagcgc tgttttctat
      481 gttttggttg ccatgctggg tgtttgcttg atggcgacaa agggagatct ctctcagtta
      541 tccatgacgc cgctagctct tataacaggt ttgctgagtg ccatgggtgt tatgtttaat
      601 gttatcttgc cccaaccttt tgctaagcgt tatggttttg ttcctacggt tgggtggggg
      661 atgattttgg caggtttgtt tagcaatgtc ctctcgccgg tttatcagct ttcctttact
      721 cttgatattt ggagtatctt gatttgcctc attatcgctt tctttggaac ggcttttgct
      781 tttttcattt ccatgaaggc tgtgtccttg gtttctcctt tggtggttgc cgttatcagt
      841 gccagtgaac ctctctcttc tgctctcttg agtgttttgt tcttaggatt agtagtggat
      901 tggtccctcc ttctagctat agccttgatt attttaccca tgatattttt gtctatagaa
      961 gaagcgaaag aaagtagata aaaagtcttt tcttaattct aaaagtgtgc tatactagaa
     1021 tagtcaataa aacacgggga gatagttgtg aagagtgttt ttaggttgta tcggtagggg
     1081 cttgctttta ccgacagcac gttttatctc acatccctaa aaaatcatac ctaaaaacag
     1141 acaaaaaggt ttcaaatttg aggccttttt tggtagaata ggtatcatta taaagaatga
     1201 gaggcctttt atgactgcta caaaaatgaa tgctcaagaa attatccaat ttatcgccaa
     1261 tgctgaaaag aaaaccagtg tcaaagtaac ctttgagggg caactcgcaa ctgctgtgcc
     1321 tagctctgtt gtcaaactag gaaatgtcct attcggagac tggaaggacg tggctccgct
     1381 tcttgaaggt ttggtagaaa atcaagatta tgttgtcgag caagatgctc gtaattctgc
     1441 agttcctttg ctagataagc gtgctatcaa cgctcgtatc gagccaggtg ctattatccg
     1501 tgaccaggtg gaaattggtg acaatgctgt tatcatgatg ggagctgtta tcaatatcgg
     1561 tgctgaaatc ggtgctggaa ccatgattga catgggtgcc atccttggtg gccgtgctat
     1621 cgttggaaaa aatagccacg ttggtgcagg tgcagttttg gcaggtgtga ttgagccagc
     1681 tagtgctgaa ccagtccgtg tcggagacaa tgttcttatc ggtgctaatg cagtggttat
     1741 cgaaggagtc caaatcggta gtggttcagt tgtcgctgca ggagctatcg ttacccaaga
     1801 tgtcccagaa aacgtggtag tagcaggtgt tccagctcgt atcatcaaag aaattgatgc
     1861 ccaaacccaa caaaaaacag cgctagagga tgcgcttcgt accttgtaat tgtaaaagta
     1921 aaaaagaggc ggaacccttt ttccagcctc tttctgctat atcggaggac agatagatgt
     1981 tagatttgat tcagactaga cgagctttac accagattcc agagattggc ttggaggagt
     2041 tcaagactca ggcttatttg ctggatgtga ttgagaaatt gactacgggc aaggattttg
     2101 ttcaaattcg tacttggcgg acagggattt tggtctacct gcagggaagt cagccggagc
     2161 gaaccattgg ttggcggaca gatattgatg gcctgcctat cgtcgaacaa acaggtctac
     2221 cttttgcttc tcaacaccaa ggtcgcatgc atgcctgtgg ccatgatttt catatgacca
     2281 ttgccttagg ctgtcttgag cgcgcccttg aggagcaacc caagaataat ctgctctttc
     2341 tatttcagcc tgctgaagaa aatgaagctg gtggtatgct catgtatgag gatggtgctt
     2401 ttggagattg gttgccaaac caattctatg gtctccatgt tcgtccagat ttgaaagtcg
     2461 gccagattgc gactaatact catacactct ttgcaggaac ttgcgaggtg aagatccgtt
     2521 tcaaaggcaa aggtggccac gcagcttttc ctcatgaagc caatgacgcc ttggtggcgg
     2581 ctagttactt tgtgacccaa gtgcagtcag ttgttagccg caatgtcaac ccaatcgagg
     2641 gagcagtggt gaccttcggc gtttttcaag ctggaaccac caacaatgtc atcacagaca
     2701 cagctttttt acatggaact attcgtgcct tgacacagga catgagtctc ttggtgcaaa
     2761 agagggtaaa aacagttgca gaaggggttg cagctgcctt tgatatggaa gtcgaagtgg
     2821 aactcaaaca aggtggatac ctacctgttg agaacaatcc agccttggcg cgtgaactga
     2881 tggacttctt tgacgaaaaa gacggaatcg agttgattga tatcgaacct gctatgacag
     2941 gcgaggactt tggttatctc ctttcaaagg ttgatggcgt gatgttttgg ctaggtatcg
     3001 atagtcccta cgctcttcat caccctcaaa tgagtcctaa ggaagaagtc ttagctattg
     3061 gggtggctgc ggtgtctagt ttcctgaaaa agaaggcagc agaatagagg gcttgtatat
     3121 gaaatcggaa ttacgcaagc aagtcttgca tgaaatgaag gctttatctc aagaacaaaa
     3181 acaggctata gaccaagctt taaccgagcg aattttacaa cacccctttt atcaagaagc
     3241 caaggtcatc gcaacctacc tctctttttc tcatgagttt caaacgcggg aactgattga
     3301 gcaggcgctg aaggacggca agaaggtttt aatacccaaa acttatccca aggggcgcat
     3361 ggactttgta gtctatgatc cgcagcagtt ggtaaaaact gcctttgcct tactggagcc
     3421 acagggagat ttggaagtgg tggatgtatc tcagattgat ttgattcatg ttcctggcct
     3481 ggcttttacg acggatggat atcggattgg atatggtgga ggttattatg accgctatct
     3541 ggaacatttt tctggtcata ctttgagtac ggttcatcct tgtcaaattc aggactttat
     3601 acctgaaaac catgatattc ctgttcagga ggtattaatt gatgaaggaa atctttgata
     3661 gacgttaccc tgtgacgagt ttcttcctct tagtgacggc cttggtattt ttactaatgt
     3721 tggtcactgc aggcggaaac tttgacaggg cagatacatt atttcgattt ggagccatgt
     3781 atgggccagc tattcgcctc tttcccgagc aggtttggcg tctcttgtct gccatttttg
     3841 ttcatattgg gtgggaacat ttcattgtta atatgctttc actttattat cttggaaggc
     3901 aggtagagga gattttcggc tctaagcagt tcttttttct ctatctctta tcaggaatga
     3961 tgggcaatct ctttgttttt gtatttagtc ctaaatcctt agcagcaggc gcctctacct
     4021 ctctttatgg gctatttgcc gcgattattg ttcttcgcta tgcaactcgc aatccttata
     4081 tccaacagct agggcaatcc tatctgacac tttttgtggt taacattatt ggaagtgttc
     4141 tgattccagg aatcagccta gcaggccata tcggtggtgc agttggtggc gcatttctag
     4201 cagttatctt tccagttaga ggagaaaaac ggatgtataa caccagccag agattaggag
     4261 cggtagtctt gttcgtagga ctcgccattt tgcttttcta caagggaatg ggaatgtgac
     4321 gaagttgaaa gaaaatttgt gtaatgaaaa aagataaggc attttttgaa ccttatcttt
     4381 ttattttatt ttattccttc tcagtcaatt cttttccaag ttggatgagg taattcttca
     4441 aatcatcttt gacttgtggg tgtttgaggg cgtagtcgat ggatgttttc atgaagccaa
     4501 acttgtctcc gacatcgtaa cgagccccat tgaactcacg agcaaataca cgttgtgttt
     4561 tattgagggt gtcgattgca tctgtcagct gaatttcatt tcctgcacct ggagcttggt
     4621 tttcgaggat ttggaaaatt tcaggcgtga ggaggtagcg tccgataata gcaaggtcgc
     4681 taggagcgtc ctctggagct ggtttttcaa caaaggtttc aacactgtaa agaccatctt
     4741 ttccttcgcc ttgcggagca ataaccccgt aagcagatac ttcgtcatga gggactggca
     4801 tgacagcgat agtagacgcg tgggtacgct cgtagtcatc catgagttgt ttggtaagtg
     4861 gaacagcctt ttcgtctgtg atatccatca agtcatcacc aagcataacg acaaaaggtt
     4921 catttccgac gaaagcctta gcttgcaaaa cagcatctcc gagaccgcgt ggatgagttt
     4981 ggcggataaa atgcagacgc atgtcagttg ttttatcaac tagcttcaaa agatctgttt
     5041 tccctttttc tttgaggtta tattccaatt cgaagtttga atcgaagtgg tcctcaatag
     5101 aacgttttga tttaccagtg acaactagaa tatcttcaat acctgatttg agagcctctt
     5161 ccacgataaa ctggatagtt ggtttgtcta cgattggcaa catttctttg gcaagggcct
     5221 tggttgctgg taaaaatcga gttcctagtc cagcagcagg gatgactgcc tttctaactt
     5281 ttgatgtcat aagaatcctt tctatagagg gttaagacca ctcattttct gctttaaatt
     5341 cattgttcat gatgtcataa atggtatctt tgatattggt ttcgtgataa ataacttggt
     5401 aaatggcctg tgtaatgggc atatagactc caagttcttg ggctagttca taggctgctc
     5461 gagtcgttga aattccttcg attaccatgc ccatattagc ttctatatca gctagggatt
     5521 ctcctcgtcc gagagcatct ccagctctcc agttacgaga gtggatggaa gttcccgtta
     5581 cgatcaaatc tcccacacca gataagccgc tataggtcaa tggactggcc ccgagagcca
     5641 cccctaggcg agtaatttca gccaagcctc gagcgatgat ggctgcctta gcattatcac
     5701 caaatccaag accatgtaaa gctccagcac cgacagcaat aatattttta agagcaccag
     5761 cagtttcaac cccgataaca tccgtattgg tataaagtcg gaagtagtga ttactaaata
     5821 gctcctgaac gtattgagct gtttgtaaat ctttagaagc agcagttatt aaagttaggt
     5881 cacgcacaat ggtctcttct gcatgactag gtcctgaaac aacgacgata tcactacgga
     5941 gatgttcagg aatttcttct tcaagaatga ttgataatcg tttatggcta tcaggttcta
     6001 atccctttga tgcgtgcatg atgataacct tatggtccaa ggtttgtgca acttgctggg
     6061 caacaagtcg tgtcactttt gttgggacaa caaacaaaat tgcatccaca tctttcaatg
     6121 tttctgctaa gtcggtgtag gcaatgatat tttcgtctag aacgacatct ttaaagtagt
     6181 gcttattagt atggtgtgta ttaatttcat tgatttgctc gggaagattt ccccaaatac
     6241 gtacctcgtg tccattgtca tttaagactt gtgaaagggc ggttccccaa gaaccaggcc
     6301 ccaagacggc gacagtttgt tttttcatga tttcctcctt gatagggttc tttctcttat
     6361 tctatcatat tctaggggat tttgcacttg cattactggg gtatggtgga aaaatacaca
     6421 aaaaccgaga cgaagggata aactcttagt ctttagttaa aatgagaaga aatctgatag
     6481 atagtttgat tcagtatatg cttataatag aggtaaacaa ctcaggaggt tcttgtaggt
     6541 tgcgagagtt tggcgaaaaa attaaaagat tacgtttggc taaaaaaatc agtcgttcag
     6601 aattttgtgg tgatgagtct gaattaagta tccgtcaatt aattagaatt gaaaatggag
     6661 aatccagacc aacactaaca aagttaaaat atatcgctga acgtttgggg gttgaagatt
     6721 acaagttgat gccaagttat atagagttgg ataaggaata cctagaattg aagtatttct
     6781 tgatgaggac tcctacatac gaagatgaaa ctatcgtcca aaagaaagag agtgtttttg
     6841 ctaagatttt tgaagagtat tatgataggc tacctgagga agaaagattt atcatcccaa
     6901 attattcata tctggcacta acgaactaca cagttcaaaa attaccagaa aagctagttg
     6961 aaatactgtc cttctggtga ttttgcttaa tttctgatgt tcgagacatg attgaggaga
     7021 tttgtcttga ttttttccta aaaaagggca gactcctccc ttggtttgat gctcgatttt
     7081 taaaataagg tgacttattc tgttaggcat gtttccccct gccctctagc tctatatgga
     7141 acagggagta tcagatttat ctggcagatt gtatcaaata aggcttgaaa ctgttttgaa
     7201 taggcgtata gacttgagaa tttgagaata tgtcgtctag cagtagagac atattttccg
     7261 gcaatatgaa ggaagagacg tcgaaaacgc ttgatagtca aggcctttac ttcatcacca
     7321 gctagctgct ttaaaaagag gtagagatta taagccagac agcccatcat catacgtact
     7381 tcattcttaa tcatggtcga actatctgtc ttatccccaa agaatcctgc tttcctttct
     7441 ttgataaagt tttcagcatt ccctcgttca cgatagaatt gaaacagctc ctcactatct
     7501 acactgctta aattgttcaa atgaactttt ttacaaccat aaaaaagagc acttaatcgt
     7561 actcttctct tttaaggatt agttgaatta attcaaatcc actagttctc ctgtttctag
     7621 gtagactagg cgttcacaaa tgttagcaat gtaatcagcg aagcgttcca gatgccctat
     7681 gatataaagg tattgagttc cattgggaat tgaggtttct tggtctttca taagtccaat
     7741 gatttccttt gataaggcat aataatattg gtcaatctgt tcatcttttt gagcaatact
     7801 aatagctttt gaggcttggt gcaaaggaaa ggcaaccaat aaatcggcta gcatgctgag
     7861 ggataattta cccatttggt gtaactgttc ttcgtcaggg gctagttgat tttcttttag
     7921 ttgcaaaaca gctttggcaa tgcctgccat atggtctccc atacgttcaa ggtctgaaca
     7981 agaagacatg atgctaatca caaatcgaag gtcagacact tgtggctgct gcaaggccaa
     8041 caaacgggca caggtcaatt cgatagcgct ttgaccttgg ttgatagcat gatccttatt
     8101 gataattagc tctgccatct ccttgtcttt ggaggctaag gccagtaagg cttttgaagc
     8161 tgtttcaagg acaagttgcc ctagtcctaa aaaggattgt tctaattcat gcaattctaa
     8221 gtcaaattga tttctcatag gtttttcctt ccttatccga accgtcctga aatatagtct
     8281 tctgtgcgct gatcttttgg attggtaaac acgtcaacgg tatctccaaa ttcgcaaatt
     8341 tctcctgtta agaaaaaagc agttttatct gaaatacgtg aagcttgttg catgttatgg
     8401 gtaacaatga taatcgtata atcctttttt agttgctgaa tgaggtcttc aatttttaaa
     8461 gtggagatag ggtctaaggc tgaagtcggc tcatccatta acagaatatc aggttctact
     8521 gctaaagctc gcgcaatgca aaggcgttgc tgctgaccgc cagataagga catggcactc
     8581 tttttaagat catctttgac ttcttcccaa atggctgccc cttttaaaga tttctccact
     8641 aaggcatcta attgtttttt gtctcgaata ccatgtgtcc ttggaccata agccacgtta
     8701 tcatagatag acatggcaaa gggattaggc tgttgaaaaa ccatccctac acgcttgcgt
     8761 agctgattaa ggttgaattt gctactataa atatcttgct catctaagag gacttggcct
     8821 tcaatatggc aagaaggaac caaatcgttc atccgattaa gggtttttag aaaagttgat
     8881 ttgccacaac cagatgggcc tatcaaggca gtaatctgtc tttctggtaa ttgaatcgaa
     8941 atatttttta aggcttgaaa atccccgtaa aataagtcta ggtgtctaac tgaaaatgtt
     9001 cccatactag gaagctcctt tcacaagttt tcgagataat aagcttgata gagtatttat
     9061 cattaaaaca gtaataatca aaatcacacc ggtagcatag gcttcattga catgtagccc
     9121 ctcacttgac agcatataca tatgcagggc tagagaacga cctgaagaca tgagactact
     9181 tggcgtattg gtagaggtac ctaatgtata catgagggca gctgtctcac caacgatacg
     9241 gccaatagct agtatcactc cagctaaaat acctggcatg gcaactggta gaacaattct
     9301 aaaaacagtc cgtaacttac ctgccccaag tccataactt gcttgacgca tgctatcact
     9361 aacagataaa agggcttctt ctgttgagcg aataatgact ggcaacacca tgataactga
     9421 ggttaagatt cctgataaca gagagtattg aaaacctaag aagactacaa agaagagcat
     9481 gccaaacaga ccaaaaacaa tggaaggaat cccagacaag gtatctgagg ccaatcgcat
     9541 gattttaaca caaagggaat ctttttttgt atattccaca agataaaaac cagcaaaaat
     9601 ccctatgggc aaggctaaaa gaagagcacc aaagaccaga ataacggtgg aaataatcgc
     9661 tggcataagg gaaatgttct cagaagtata agtccaagaa aagagggata gacttagatg
     9721 aggtaagcct ttgatgagga taaaaccaat gattaaaaag agagagccaa aggttaaagc
     9781 tgaaaaacaa taaacgagaa gttttagcag gtatttactc ataagatgat tttcctttca
     9841 agtaggcaaa gtaggcatta atcaagagaa taaggaaaaa gagaactgct gaggttgcaa
     9901 taagggcttc cctatgctga cctgatgcgt aagccatttc cagaacaata ttggttgtta
     9961 aggttctggt tcctgaaaag agtccacttg gaataatcgg ctggttgcct gccaccaaaa
    10021 taactgccat ggtttcacct actgcgcgac cgattcctaa aataactgct gataaaatac
    10081 cagatctcgc agctggcaag atgacactaa aaatactccg ttcatgacta gctcctagag
    10141 ccaagctacc agaataatac gttttgggaa ctgttcggat agcagattct gacaaactga
    10201 taatggttgg caaaatcatt attcctaata gtaacgaagc ggttaggaca ctcatgccat
    10261 ttcctaaaaa gcttctaatc caaggcacca ataattgtag gccgaaaaaa ccataaacaa
    10321 tagatggaat ggccgccatc aagttgatag ctgattttaa gaagccatag acgggctttg
    10381 gacaataata aaccataaac accgatgtca agatgcctgt tggcacccca atcacaatcg
    10441 ctcctaaggt aattaataag gaaccaacga tcattggtaa aataccatag cttgccggaa
    10501 tgttcgttgg cgaccaatca ctgcctaata aaaaacgggc aaagccgtag ttagccatga
    10561 aaggtaagcc attactaaaa ataaagaaac agattagcaa aatagctaca acagctactg
    10621 ttgcactcat gaaaaaaatt gccctaaaaa ctgcttcttt gaaggcttgt tttatcacat
    10681 cttgtccttt ctagtgaaga aagtaaggga gatacgacac ctccctactt gccttcttta
    10741 tcttattgta cgatgaaacg tctgcatctc tttagagatt tatggagcaa acattttatt
    10801 taatcttgtc ccaggtggtt aatttgccac taaaaacgtc tgcaagttca gccatactga
    10861 cttggcttgc cttattgtca ttattgacca caacagcaat accgtctaaa gcaatagcat
    10921 catgggtgag actcttacct tcttcaggag ttaattccct agaaaccata ccaatatcag
    10981 cggttttctc cttaacagcg gtaatacctg ctgaagaccc attagaggta atatcaatcg
    11041 taacttctgg attttctttt ttataagctt ctgctaattt ttccattaaa gaagatactg
    11101 aagtggaacc tacaacagac aacttgcctg ataagtgttg gcttgtatat tctgtggttt
    11161 cggttttagc ttcaataaat ttattatctg tgaccacttg ttgaccttgt ttggagtgga
    11221 taaagctgat aaaatcttga cctagcttgg aaagattaga agaccaaaca atgttgaagg
    11281 gacgttgaag agggtattca ccatctaaaa ctgtgtctcg actagccttg acaccatcaa
    11341 tctctaaagc cttgacagat ttcgttaaag atcccaagga gatgtagccg atagcattag
    11401 cattcccttg aactgctgag agaacacctt ctgtactatt ttgaatcaca gctgttttgg
    11461 cagtgttgtc aattttttta tcaccgtctt ttttgagaat ccctgtgatt tctgtgaagg
    11521 caccccgtgt tccagagcca ttttctcgtg aaatcacctc aatcgttcct gaagctgact
    11581 gtttggaagc agctgactga ttgccacagg caacaagccc aaatcctgat aagccaatgg
    11641 ctgcaagagt aagcattttt ttgaatttca taataatcac ctttatctct atgtattttt
    11701 cttgtgtagg cttactacat ttatagtcta acaagtcttt gtaaaggttt atccctgatt
    11761 catgtaaaga ttgtgtaaag aatcaaaaaa agccactttt gaaaaatggc tgctcctaaa
    11821 aatagcttta aaaattatta gtcctgtgcg aaagattggt taggaagaaa aatcgtgaag
    11881 caactgcctc tgccaagctg actcgtcacc gtgacttggc cacctaataa ttgactgagt
    11941 tctttgacaa tggcaaggcc aagaccagtg ccaccagttt gtctgcttcg acctttatta
    12001 actcggtaaa aacgttcaaa aatacgatcc tgctctaatt gactaatacc aatccctgta
    12061 tctgatacag aaatcttaat gccttcgttc accttttggg tcttgacctc aattttcccc
    12121 ccttgttcag tgtaacggat ggcattggat aaaagattga gtaagatttg ggaaagtaat
    12181 tgactatctg atacgagggt gacatcatct ggcacctgca cctttagctg taaatccttc
    12241 ttcttgagct gaggttgcaa gctttgagtc aaatcctgta caaattctgc caaagaaagg
    12301 gtcgtccatt gtataggcat ttgttgagcc ttagataagg taagaagatg ctcaacaata
    12361 tgctcaagac gcaaactttc tttgtaaata atgtctagaa agtcatcctt gagcgcttct
    12421 tcttcagctg acatcccctt aatggtttca gcaaagccct taatcgaagt aactggtgtc
    12481 ctcaattcat gggaggcatt tgagacaaag gctaaattta acttttcata agttctaatc
    12541 gttgttaaat catatagcaa gacgagcaca gcttccacag attgggtggg gctaaaaacg
    12601 ggaactgctg tcacttctaa aatcaagtca ccctcatgaa acccacttac ttcttgtttt
    12661 aaccttgttt tttgatcaaa ggcttggtga actaaattcc gaatatccat ccgtttgagg
    12721 tcatcaagtg aacttatgtc gccgtccaca tcgggaaaat aatgaggcag agagcgactg
    12781 gataataaca tctgaccttg agcggaaact aaaaacgtcc ccatggttag gtgcgacaga
    12841 agaacctcca ttgtttcggc tagatccttg tattgctgat cctgttggga gactttggtt
    12901 tttaggccag acacatactg agccaaagac tttaagtctt cttgcccttt ttctaaaaag
    12961 tattcactac tggtcaagag aggttggtgc aaggtctcaa aagcaacttc ccatttccaa
    13021 aggcaaaaga gccagtagcc acctagtccc aaagaaaggg ctagaagaaa gagaccgatg
    13081 cctttactga tccaagttaa tgccatccct gcaatcagaa tgaggctaac acttagattg
    13141 actagccaaa attgaaggta gcgtttcatc tataactcct tgaacttata accataaccc
    13201 cgaatggttc gaataaattg aggggcttta ggattgtctt caattttttc cctcaactta
    13261 ccaatatgaa cgtccaccaa acgtgtttcc tgcccaaagt cataccccca gatacgttcc
    13321 aaaagacgct ctctagtcag tgtcatgttg ggatgtttca taagatagag caagagttca
    13381 aattcttttg gggtcaaact cagtaactta ttcgccttgt agacttcatg acgctcaggg
    13441 tatactttca aggtcccaaa tagccaagaa tcgtcagcga tattatctga atcatctcct
    13501 tcttgttctc ctttagttcg cctgaggaca gccttgacac gcgccagcaa ttctctaggg
    13561 ctaaaaggct tggtcaggta gtcatcagcc cctaattcca aggccaaaac cttatcaaat
    13621 tcatcacttt tcgcagaaac catcataatt ggagttttga cgcctttggc tctcagccgc
    13681 ttacaaactt ctatgccatc taattgtggt aacatgatat caagcaagat aaaatcaaag
    13741 ggttctgttt ctgccaaagc taaggccttc cgtccatttg tcaccaattg agtagaaaag
    13801 ccttccttac ttaaatggta gtcaagcaat ttcagaatgt gttcttcatc atccactaat
    13861 aagacttgtt ttgtcatcta ttatctccta ttggtaacat tataacacaa ttatcagaaa
    13921 tcctaacatt gctaaatcag attaaatttg cctatcaaga ctagtatctg gtcaaacgct
    13981 caatcatctc cttgtgctct ggataggtcg ccagtagatc taccctttca aataattcaa
    14041 aatcctcaaa ttcaaaacca ggagcaacaa gacaagaaac cagagcataa tccttatcaa
    14101 ctgttgatcc ccaaatagtg cccttaggaa cacagtagtg aagttgttgc cctttggata
    14161 tgtccaggcc taaagtgact gcttcgtagt gaccatctgc tgtaatcatg tgaacagtaa
    14221 gtggggatcc tgcatgaaaa taccagattt catctgctgt caatcggtga aaatgtgaag
    14281 gattcgtttc ttctaataag aaataaatac tggtataaag cgcccttccc ttaccagcaa
    14341 ggtttatagt gtctgaagct ttttttgttt gtctaaaata gccaccttca acatggggag
    14401 ctaactctag agttcttatc aagtcttctt tatccgtcgg agccaatggg ttgaagtaac
    14461 tcttgttcaa agtggtttta cgatttcaag aactcctctc agttctgagg acacggtaat
    14521 gattgatgcg acggaagtac aaatcaatcg ccctaaaaaa agaattagcg aattattctg
    14581 gtaaaaaaaa tgccacgcta tgaaggctca agcgattgtc acaagtcaag ggagaattgt
    14641 ttctttggat atcgctgtga actattgtca tgatatgaag ttgttcaaaa tgagtcgcag
    14701 aaatatcgga caagctggta aaatcttggc tgacagtggt tatcaagggc tcatgaagat
    14761 atatcctcaa gcacaaactc cacgtaaatc cagcaaactc aagccactaa cagttgaaga
    14821 taaagcctat aaccatgcgc tatccaagga gagaagcaag gttgagaaca tctttgccaa
    14881 agtaaaaacg tttaaaatga tttcaacaac ctatcgaaat catcgtaaac acttcggatt
    14941 acgaatgaat ttgattgctg gcattatcaa tcatgaacta ggattctagt tttgcaggaa
    15001 gtctattatt tggttaggtg aattagtgaa gcgtttaggc aagtgtctct ggttacgacg
    15061 tcatggactc taaatcgatt atatttaggg gtcatgacta gtgaagcagt tagctagttc
    15121 gcatataagc ggctagcgtc taacaattag gaactttagt tccaataact ttaagattac
    15181 gacgttttag gacataaatc gatcatattt atgtcctaaa actagtgaag cgcctagcca
    15241 aagtccgaat aggatttggc gttagttact tagattgctt tgcaatcaag taactttggc
    15301 gatttacatc ttctctggcg cttctactcc aagcaagcga agggcttctt tgagaacgac
    15361 tgcggttgcg tagctgaggg ctagacggct gtcgcgttct gggctttcat ccaagatacg
    15421 tgtatgtgca tagtatttgt taaaggattg agccaggcta attgcaaatt tagcaatgat
    15481 agaaggttca aagttatctg ccgcacggtt gataatacgt gggaagtctt gaatgagttt
    15541 aatgatttcc cagctttcag tatcattcaa gctatagttg ccagctgttt ctggtttgaa
    15601 atcggctttg cgtaagatag attggatacg agcgtaggca tattgaacgt aaggtccagt
    15661 ttcaccctcg aaggatacca tagcctctag gtcgaagtcg tatccatttg tacggtcggt
    15721 tttgaggtca tagaatttaa tggctccaac cccaacagca tgtgctactt ggtctttgtt
    15781 ttctagttca ggatttttag cctcgatttg gaccttggca cggctaacag cctctgcaac
    15841 agtaggctct agcaagatga cattcccttt acgagtagag agcttcttcc cttcttttgt
    15901 aaccagacca aaaggaacgt gggtaatgtc gtcactccaa tcgtagccca tctcttgcaa
    15961 gacagctttg agttgtttaa agtgggcaga ttgctcttga ccaacgacgt agatagattt
    16021 agcaaattgg tattcatttt tacggtagag ggctgcagcc aagtcacgtg tgatatagag
    16081 agttgcacca tcagacttct tgatgagagc tggatgttca attccatatt tctcaagatt
    16141 cacaacttgg gcaccttctg attcaagaag tagtcctttt tcagaaagaa tgtctacaac
    16201 tgcatccatc ttatcattgt agaaggcttc tccgttatag ctgtcaaatt caaccttcaa
    16261 ttcattgtaa aggcggttaa attccactaa actttcatcg cggaaccatt gccaaagagc
    16321 gagagcttcc tcatctccat tttcaagttt acggaaccat tcgcgcgctt cttcatccaa
    16381 gctagggtca ttttcagctt cagcgttgat gcggacatag agtttaagga gttcatcgat
    16441 tggatgagct tttacagctt cttcgtcgcc ccatttttta taggcaacaa tcaacatccc
    16501 aaattgttta ccccagtctc ccaaatggtt gaccttgacc gtttgataac cgattttttg
    16561 gaaaatatgt gacaagctat ctccgataac agttgaacgc aggtggccaa tagaaaatgg
    16621 tttagcgata ttcggactag acatgtcgat aacaacattt tcttgtttac caatattttg
    16681 gtcagcatag tgttcttttt cagtggtaac agcttgcaat acttgagcag aaatggcaga
    16741 tttatcaagg aaaaagttaa cgtaaggtcc tgttgcgaca actttttcaa aggcttggct
    16801 gttcattttt tcagccagtt cagccgcaat catttgtggt gctttacgtt cgacttttgc
    16861 aagagaaaaa gcagggaaag caatgtctcc catttctgag tttttagggg tttccagtaa
    16921 ctttaaaaca gcctcttggt ccaggctatc aatgatgcta gataattcgc tagcaatcaa
    16981 ttcttttata ttcattaaga gctccttttt ggacttttct actattttat cacaatttta
    17041 aagaaagaag aaaaaatttt tgaaatctcc tgtttttttg gtataatatg gttataaata
    17101 tagttataaa tatgcacgca agaggatttt atgagaaaaa gagatcgtca tcagttaata
    17161 aaaaaaatga ttactgagga gaaattaagt acacaaaaag aaattcaaga tcggttggag
    17221 gcgcacaatg tttgtgtgac gcagacaacc ttgtctcgtg atttgcgcga aatcggcttg
    17281 accaaggtca agaaaaatga tatggtgtat tatgtactag taaatgagac agaaaagatt
    17341 gatttggtgg aatttttgtc tcatcattta gaaggtgttg caagagcaga gtttaccttg
    17401 gtgcttcata ccaaattggg agaagcctct gttttggcaa atattgtaga tgtaaacaag
    17461 gatgaatgga ttttaggaac agttgctggt gccaatacct tattggttat ttgtcgagat
    17521 cagcacgttg ccaaactcat ggaagatcgt ttgctagatt tgatgaaaga taagtaaggt
    17581 cttgggagtt gctctcaaga cttatttttg aaaaggagag acagaaaatg gcgatagaaa
    17641 agctatcacc cggcatgcaa cagtatgtgg atattaaaaa gcaatatcca gatgcttttt
    17701 tgctctttcg gatgggtgat ttttatgaat tattttatga ggatgcggtc aatgctgcgc
    17761 agattctgga aatttcctta acgagtcgca acaagaatgc cgacaatccg atccctatgg
    17821 cgggtgttcc ctatcattct gcccaacagt atatcgatgt cttgattgag cagggttata
    17881 aggtggctat cgcagagcag atggaagatc ctaaacaagc agttggggtt gttaaacgag
    17941 aggttgttca ggtcattacg ccagggacag tggtcgatag cagtaagccg gacagtcaga
    18001 ataatttttt ggtttccata gactgcgaag gcaatcaatt tggcctagct tatatggatt
    18061 tggtgacggg tgacttttat gtgacaggtc ttttggattt cacgctggtt tgtggggaaa
    18121 tccgtaacct caaggctcga gaagtggtgt tgggttatga cttgtctgag gaagaagaac
    18181 aaatcctcag ccgccagatg aatctggtac tctcttatga aaaagaaagc tttgaagacc
    18241 ttcatttatt ggatttgcga ttggcaacgg tggagcaaac ggcatctagt aagctgctcc
    18301 agtatgttca tcggactcag atgagggaat tgaaccacct caaacctgtt atccgctacg
    18361 aaattaagga tttcttgcag atggattatg cgaccaaggc tagtctggat ttggttgaga
    18421 atgctcgctc aggtaagaaa caaggcagtc ttttctggct tttggatgaa accaaaacgg
    18481 ctatggggat gcgtctcttg cgttcttgga ttcatcgccc cttgattgat aaggaacgaa
    18541 tcgtccaacg tcaagaagta gtgcaggtct ttctcgacca tttctttgag cgtagtgact
    18601 tgacagacag tctcaagggt gtttatgaca ttgagcgctt ggctagtcgt gtttcttttg
    18661 gcaaaaccaa tccaaaggat ctcttgcagt tggcgactac cttgtctagt gtgccacgga
    18721 ttcgtgcgat tttagaaggg atggagcaac ctactctagc ctatctcatc gcacaactgg
    18781 atgcaatccc tgagttggag agtttgatta gcgcagcgat tgctcctgaa gctcctcatg
    18841 tgattacaga tgggggaatt atccggactg gatttgatga gactttagac aagtatcgtt
    18901 gcgttctcag agaagggact agctggattg ctgagattga ggctaaggag cgagaaaact
    18961 ctggtatcag cacgctcaag attgactaca ataaaaagga tggctactat tttcatgtga
    19021 ccaattcgca actaggaaat gtgccagccc actttttccg caaggcgacg ctgaaaaact
    19081 cagaacgctt tggaaccgaa gaattagccc gtatcgaggg agatatgctt gaggcgcgtg
    19141 agaagtcagc caacctcgaa tacgaaatat ttatgcgcat tcgtgaagag gtcggcaagt
    19201 acatccagcg tttacaagct ctagcccaag gaattgcgac ggttgatgtc ttacagagtc
    19261 tggcggttgt ggctgaaacc cagcatttga ttcgacctga gtttggtgac gattcacaaa
    19321 ttgatatccg gaaagggcgc catgctgtcg ttgaaaaggt tatgggggct cagacctata
    19381 ttccaaatac gattcagatg gcagaagata ccagtattca actggttaca gggccaaaca
    19441 tgagtgggaa gtctacctat atgcgtcagt tagccatgac ggcggttatg gcccagctgg
    19501 gttcctatgt tcctgctgaa agcgcccatt taccgatttt tgatgcgatt tttacccgta
    19561 tcggagcagc agatgacttg gtttcgggtc agtcaacctt tatggtggag atgatggagg
    19621 ccaataatgc catttcgcat gcgaccaaga actctctcat tctctttgat gaattgggac
    19681 gtggaactgc aacttatgac gggatggctc ttgctcagtc catcatcgaa tatatccatg
    19741 agcacatcgg agctaagacc ctctttgcga cccactacca tgagttgact agtctggagt
    19801 ctagtttaca acacttggtc aatgtccacg tggcaacttt ggagcaggat gggcaggtca
    19861 ccttccttca caagattgaa ccgggaccag ctgataaatc ctacggtatc catgttgcca
    19921 agattgctgg cttgccagca gaccttttag caagggcgga taagattttg actcagctag
    19981 agaatcaagg aacagagagt cctcctccca tgagacaaac tagtgctgtc actgaacaga
    20041 tttcactctt tgatagggca gaagagcatc ctatcctagc agaattagct aaactggatg
    20101 tgtataatat gacacctatg caggttatga atgtcttagt agagttaaaa cagaaactat
    20161 aaaaccaaga ctcactagtt aatctagctg tatcaaggag acttctttga caattctcca
    20221 cttttttgct agaataacat cacacaaaca gaatgaaaag gagctgacgc attgtcgctc
    20281 ccttttgtct attttttaag gagaaagtat gctgattcag aaaataaaaa cctacaagtg
    20341 gcaggccttg gcttcgctcc tgatgacagg cttgatggtt gctagttcac ttctgcaacc
    20401 gcgttatctg caggaagtat tagacgccct ccttactggg aaatatgaag ctatttatag
    20461 tatcggggct tggttgattg gtgtggccgt agtcggtcta gttgctggtg gactcaatgt
    20521 tgtcctcgca gcctatattg cccaaggagt ttcatccgac cttcgggagg atgccttccg
    20581 taaaattcaa accttttctt atgctgatat tgaacaattt aatgcgggaa atctagtcgt
    20641 tcgaatgaca aatgatatca accagattca gaacgttgtc atgatgacct tccaaattct
    20701 tttcagactt cccctcttgt tcatcggttc gtttatccta gcggttcaaa ccttaccttc
    20761 tctgtggtgg gtgattgttc tcatggtagt cttgattttt ggtttgactg ctgtcatgat
    20821 gggaatgatg gggcctcgtt ttgccaagtt tcaaaccctt cttgagcgca tcaatgccat
    20881 tgccaaggaa aatttacgtg gcgttcgtgt ggtcaagtcc tttgtccaag aaaaagagca
    20941 atttgctaag tttacagagg tctcagacga gctttttggt caaaaccttt acattggtta
    21001 tgccttttca gtagtggaac cctttatgat gttggttggt tacggggcgg tcttcctctc
    21061 tatttggctg gtcgcgggaa tggttcagtc ggatccgtct gttgttggtt ccatcgcttc
    21121 ttttgttaat tacctaagcc agattatctt taccattgtt atggttggat ttttgggaaa
    21181 ttctgtcagc cgtgccatga tttccatgcg tcgtattcga gaaattcttg acgcagagcc
    21241 agctatgacc ttcaaggata tcccagatga agagttggtt ggaagcctta gctttgaaaa
    21301 tgtgaccttt acctatccaa tggacaagga accgatgctg aaagatgtga gctttactat
    21361 tgaacctggt caaatggttg gtgtagttgg agcgactggt gcaggaaagt caaccttggc
    21421 tcaattgatt ccacgtctct ttgatccaca gaacggggcc attaaaatcg gtggcaagga
    21481 tattcgagaa gtgagtgaag gaaccctgcg taaaacagtt tccatcgttc tccaacgtgc
    21541 cattcttttt agtggaacga ttgcagataa cttgagacag gggaagggaa atgctactct
    21601 atttgaaatg gagcgcgcag ccaatattgc ccaggctagt gaattcattc atcgtatgga
    21661 gaaaaccttt gaaagtccag ttgaagaacg gggaaccaat ttctctggtg gacaaaaaca
    21721 aaggatgtcg attgcgcgtg ggattgtcag caatccacgt attctgattt ttgacgattc
    21781 gacctcagcc ttggatgcca aatcagagcg cttggtgcaa gaagctttga ataaggactt
    21841 gaaggggacg acaaccatta ttattgctca aaaaattagc tcggttgtcc atgcagacaa
    21901 gatcttggtt ctaaatcaag gacgattgat tggtcaaggc acgcatgcag acttggttgc
    21961 caacaatgcc gtttaccgtg aaatctatga aacacagaaa tgaaagacaa actataagaa
    22021 aagtcaatag ttttatctaa attatttctt atttcaattt gatgatttgg cgatgatttt
    22081 agagcacggc aaaaagccct tgaaaaagtc cattttttca aaggtaatcc tgtgtt
//
LOCUS       DBERDK010000029        21947 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.029, whole genome shotgun sequence.
ACCESSION   DBERDK010000029 DBERDK010000000
VERSION     DBERDK010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 21947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21947
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.029"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            94..6366
                     /locus_tag="ACL73L_001112"
                     /pseudo
     CDS             94..6366
                     /locus_tag="ACL73L_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750039.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
     gene            6362..10096
                     /locus_tag="ACL73L_001113"
                     /pseudo
     CDS             6362..10096
                     /locus_tag="ACL73L_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000472960.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ZmpA/ZmpB/ZmpC family metallo-endopeptidase"
     gene            10157..11878
                     /gene="pabB"
                     /locus_tag="ACL73L_001114"
     CDS             10157..11878
                     /gene="pabB"
                     /locus_tag="ACL73L_001114"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HGS0772105.1"
                     /translation="MHRKTVIDFRTLGERYTFIQPIKELKTRNVEEVADLLAQVESYQ
                     EQGYYVVGYVSYEAAPAFEEKLAVHKDPLLGEYLLYFTVHDRVETSPIPLTYEDIDLP
                     SNWQEVTSAENYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLSANPFAIYNRMVVEQEA
                     GYNAYVEHDEMAVISMSPELFFEQNDRELTTRPMKGTTQRGVTDQEDLEQASWLEQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVERLCQVEQYSTVWQMTSTIKSQLREDVDLV
                     KIFRSLFPCGSITGAPKIATMEIIKDLEPQPRGVYCGTIGLLLPNGRRIFNVAIRTIQ
                     LHKGQAIYGVGGGITWDSTWESEYREVHQKAAVLYRKQARFQLITTGEISQKNLLFED
                     KHLERLRKASRYFAFPFDAEDLGHKIEEECQDCEANQDYRLRISLSKSGEIEVNRQVL
                     TPLSTSFCQAQVCLQEAALNQSFTYFKTTHRPHLSLGEQEKIYHNKSGELLETSIGNL
                     VLKIAGKLYTPPIRLGILPGIYRQYLLETGQVEEKVLTLADLAQAEAIYGCNAVRGLY
                     ELSLEEN"
     gene            11879..12496
                     /locus_tag="ACL73L_001115"
     CDS             11879..12496
                     /locus_tag="ACL73L_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGS0772106.1"
                     /translation="MKLIFLHGLGQSAESWKEVRNLLTDYPSEAIELFPSGVSNYQQA
                     KERVYQYLAQETEPFVLIGLSLGAALALELSSYDLPNLRALILSGCPLKLAGNILFYL
                     QLLIFKLLPKRVFEKQGADKALMVGVSEELKTLDLTDIAGTCPYPTLLICGIKDKPNL
                     SSMRSLHKLISESQFQIISDGPHVLNKAKPKEFVEKTRSFLELLK"
     gene            12546..13424
                     /locus_tag="ACL73L_001116"
     CDS             12546..13424
                     /locus_tag="ACL73L_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HGS0772107.1"
                     /translation="MNKRLFLKMSLVTLPILALFSQPVLAEENIHFSSCKEAWANGYS
                     DLHEGEPGYSAKLDRDHDGVACELKNAPKGTFKAKQAATTQTDTTSSTASGWVKQDGS
                     WYYFDGNGNLVKNAWQGAYYLKSNGKMAQGEWVYDSSYQAWYYLKSDGSYARNAWQGA
                     YYLKSNGKMAQGEWVYDATYQAWYYLKSDGSYAYSTWQGNYYLKSDGKMAVNEWVDGG
                     RYYVGADGVWKEGQASTASSSNDSNSEYSAALGKAKSYNSLFHMSKKRMYRQLTSDFD
                     KFSNDAAQYAIDHLDD"
     gene            13551..14510
                     /locus_tag="ACL73L_001117"
     CDS             13551..14510
                     /locus_tag="ACL73L_001117"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HGS0772108.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTAGEIQGKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDRDKGTVIGAYNLNWKTLQPIKQKIE
                     KSLGIPFFIDNDANVAALGERWMGAGDNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPISCTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFDLAKEGDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENTFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            14607..15446
                     /locus_tag="ACL73L_001118"
     CDS             14607..15446
                     /locus_tag="ACL73L_001118"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HGS0772109.1"
                     /translation="MTKADTIFKENIERILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPITTLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLLKQLKTNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRVDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(15494..15664)
                     /locus_tag="ACL73L_001119"
     CDS             complement(15494..15664)
                     /locus_tag="ACL73L_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002263068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HGS0772110.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            15785..16669
                     /gene="miaA"
                     /locus_tag="ACL73L_001120"
     CDS             15785..16669
                     /gene="miaA"
                     /locus_tag="ACL73L_001120"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HGS0772111.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKRFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVLHHLIDVREITESYSAFDFVSEAKMTIEDIHSRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHLVEQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQETLYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRREQTLEEARESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            16662..17900
                     /gene="hflX"
                     /locus_tag="ACL73L_001121"
     CDS             16662..17900
                     /gene="hflX"
                     /locus_tag="ACL73L_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HGS0772112.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMEELASLAKTAGAVVVD
                     SYRQKREKYDSKTFVGSGKLEEIALMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESSTFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPTELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTVLSIMKDLDMEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKIHDLESVAILEERDYQEDG
                     EVITGYISEKNKWRLEEFYD"
     gene            17893..18516
                     /locus_tag="ACL73L_001122"
     CDS             17893..18516
                     /locus_tag="ACL73L_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HGS0772113.1"
                     /translation="MTDIKTLALKYGGYTSLDKVYLDQLLAGKTEQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKSFIRLNLSGKSFGFCY
                     ESDGFGRIFSENEEKISDDLLFEIAQIFPHQLVFEESGKIYMKPVEDEEVVSLESLTA
                     LTDLERLADGRKRLKGYSQEDLLQEAAAFSGKRYFRSENRTAMLYID"
     gene            18531..19460
                     /gene="rnz"
                     /locus_tag="ACL73L_001123"
     CDS             18531..19460
                     /gene="rnz"
                     /locus_tag="ACL73L_001123"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HGS0772114.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTIRPRKVSKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
                     GTLDAKKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTDASVRLAVNADVLVHESTYGKGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            19481..20236
                     /locus_tag="ACL73L_001124"
     CDS             19481..20236
                     /locus_tag="ACL73L_001124"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HGS0772115.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLAQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEADAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLAGETFNYK"
     gene            complement(20290..21198)
                     /locus_tag="ACL73L_001125"
     CDS             complement(20290..21198)
                     /locus_tag="ACL73L_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGS0772116.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDD
                     NHLLAHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            21262..21531
                     /locus_tag="ACL73L_001126"
     CDS             21262..21531
                     /locus_tag="ACL73L_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HGS0772117.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            21531..21911
                     /locus_tag="ACL73L_001127"
     CDS             21531..21911
                     /locus_tag="ACL73L_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HGS0772118.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQ
                     LIDLRDPAEFHRKHILGARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
BASE COUNT         7371 a         3702 c         4676 g         6198 t
ORIGIN      
        1 tagataatac ttagtaaaac tttttataac aaaggctagc aaagttaaag tttattatct
       61 attggaagtt atataaatat gtaaggaatt aaaatgaaaa aaagtacagt attgtcatta
      121 accacagctg cagttatttt agcagcctat gcccctaatg aggtagtctt agcagacaca
      181 tctagctctg aagatgcttt aagcatctct gataaagaaa aagtagcaga aaataaagag
      241 aaacatgaag atatccatag tgctatggaa acttcacagg atactgaaga aaagaaaaca
      301 acaattattg aggaaaaaga agttgttagt aaaaatcctg tgatagacac taaaactagc
      361 aatgaagaag caaaaatcaa agaagaaaat tccaataaat cccaaggaga tcatacggac
      421 tcatttgtga ataaaaacac agaaaatccc aaaaaagaag ataaagttgt ctatattgct
      481 gaatttaaag ataaagaatc tggagaaaaa gcaatcaagg agctatccag tcttaagaat
      541 acaaaagttt tatatactta tgatagaatt tttaacggtg gtgctattga aacaactcaa
      601 gataatctga ataaaattaa acaaatagaa ggtattacat cggttgaaag ggcacaaaaa
      661 gtccaaccca tgatgaatca tgccagaaag gaaattggag ttgaggaagc tattgattac
      721 ctaaagtcta tcaatgctcc gtttgggaaa aattttgatg gtagaggtat ggtcatttca
      781 aatatcgata ctggaacaga ttataggcat aaggctatga gaatcgatga tgatgccaaa
      841 gcctcaatga gatttaaaaa agaagactta aaaggaactg ataaaaatta ttggttgagt
      901 gataaaatcc ctcatgcgtt caattattat aatggtggca aaatcactgt agaaaaatat
      961 gatgatggaa gggattattt tgacccacat gggatgcata ttgcagggat tcttgctgga
     1021 aatgatactg aacaagatat caaaaacttt aacggcatag atggaattgc gcctaatgca
     1081 caaattttct cttataaaat gtattctgac gcaggatctg ggtttgcggg tgatgaaaca
     1141 atgtttcatg ctattgaaga ttctatcaaa cacaacgttg atgttgtttc ggtatcatct
     1201 ggttttacag gaacaggtct tgtaggtgag aaatattggc aagctattag ggcattaaga
     1261 aaagcaggca ttccaatggt tgtcgctacg ggtaactatg cgacttctgc ttcaagttct
     1321 tcatgggatt tagtagcaaa taatcatctg aaaatgaccg acactggaaa tgtaacacga
     1381 actgcagcac atgaagatgc gatagcggtc gcttctgcta aaaatcaaac agttgagttt
     1441 gataaggtta acattggagg acaaagtttt aaatacagaa atataggggc ctttttcgat
     1501 aagaataaaa tcacaacaaa tgaagatgga acaaaagctc ctagtaaatt aaaatttgta
     1561 tatataggca aggggcaaga ccaagatttg ataggtttgg atcttagggg caaaattgca
     1621 gtaatggata gaatttatac aaaggattta aaaaatgctt ttaaaaaagc tatggataag
     1681 ggtgcacgcg ccattatggt tgtaaatact gtaaattact acaatagaga taattggaca
     1741 gagcttccag ctatgggata tgaagcggat gaaggtacta aaagtcaagt gttttcaatt
     1801 tcaggagatg atggtgtaaa gctatggaac atgattaatc ctgataaaaa aactgaagtc
     1861 aaaagaaata ataaagaaga ttttaaagat aaattggagc aatactatcc aattgatatg
     1921 gaaagtttta attccaacaa accgaatgta ggtgacgaaa aagagattga ctttaagttt
     1981 gcacctgaca cagacaaaga actctataaa gaagatatca tcgttccagc aggatccaca
     2041 tcttgggggc caagaataga tttactttta aaacccgatg tttcagcacc tggtaaaaat
     2101 attaaatcca cgcttaatgt tattaatggc aaatcaactt atggctatat gtccggaact
     2161 agtatggcga ctccaatcgt agcagcttct actgttttga ttcgaccaaa attgaaggaa
     2221 atgcttgaaa aacccgtatt aaaaaatctt aaaggtgatg ataaaataga ccttacaagt
     2281 cttacaaaaa tagccctaca aaatactgca agacctatga tggatgcaac ttcttggaaa
     2341 gaaaaaagtc aatactttgc atcacctaga cagcaggggg cggggctaat taatgtggcc
     2401 aacgctttga gaaatgaagt tgtagcgact ttcaaaaaca cggattctaa aggtttggta
     2461 aattcatatg gttccatttc tcttaaagaa ataaaaggtg ataaaaaata ctttacaatt
     2521 aagcttcaca atacatccaa caggccttta acctttaaag tttcagcatc agcggtgact
     2581 acagattctc taactgacag actaaaactg gatgaaacat ataaagatga aaaatctcca
     2641 gatggtaagc aaattgttcc agaaattcat ccagaaaaag tcaaaggggc aaatattaca
     2701 tttgagcatg acactttcac tataggggca aattctagct ttgatttaaa tgcggttata
     2761 aatgttgggg aggctaaaaa taaaaataaa tttgtagaat catttattca ttttgagtca
     2821 gtagaagaaa tggaagcatt aagctccaat ggcaagaaaa ccgacttcca accatcttta
     2881 tcaatgcccc taatgggatt tgctgggaat tggaaccacg aaccaatcct tgataaatgg
     2941 gcttgggaag aaggatcaaa atcaaaaaca atggaaggtt atgatgatga cggtaaacca
     3001 aaaattccag gaaccttaaa taaggggatt ggtggagaac atggtataga taaatttaat
     3061 ccagcaggag ttatccaaaa tagaaaagat aaaaatagaa catctctaga tcaagatcca
     3121 gacttatttg cctttaacaa ccaaggtgtt catgcagaat ccactagtgg ttctaagatt
     3181 gctaacattt atcctttaga ttcaaatgga aatcctcaag atgctcaact agaaagagga
     3241 ctaactccat ctccacttgt tctaagaagt gcagaagaag gactaatttc aattgtaaac
     3301 accaacaaag aaggagaaaa ccaaaaagac cttaaagttg tatcaagaga gcacttcata
     3361 agaggaattc taaattctaa gggaaatgac gctaagggta tcaaatcatc caaactaaaa
     3421 gtttggggtg acttgaaatg ggatggactc atctataacc caagaggtag agaagaaaat
     3481 gccccagaaa gtaaggataa ccaagatcca gctactaaga tacgaggaca atttgaaccg
     3541 attgcggaag gtcaatattt ctataaattt aaatatagat taactaaaga ttacccatgg
     3601 caggtttcct atattcctgt aaaaattgat aacacagccc ctaagattgt ttcggttgat
     3661 ttttcaaatc ctgaaaaaat taagctaatc acaaaggata cttatcacaa ggtaaaagat
     3721 caatacaaga atgaaacttt atttgctaga gatcaaaaag aacatcctga aaaatttgat
     3781 gagattgcaa atgaggtgtg gtatgcagga gctgctcttg ttaatgaaga tggagaggtt
     3841 gagaaaaatc ttgaagtaac ttatgcaggt gagggtcaag gaagaaatag gaagcttgat
     3901 aaagatggga atactatcta tgaaattagc ggagcaggag atctaagagg aaaaatcatc
     3961 gaagtaatcg ccctagatgg agcaagcaac tttaccaaaa tccataggat taaatttgct
     4021 aatcaagctg atgaaaaagg gatgatttcc tattatctag tagatcctga tcaggattca
     4081 tctaaatacc aaaagcttgg cgaaattcct gaatctaaat ttaagaattt aaaaaatgtt
     4141 aaagatgata gcctaaacaa agagacagct gaggtggaaa ataacctttt agtagataat
     4201 cagtctatcg aaggaaaatc tctctttaat attcataaaa ctatttcaac aattagagac
     4261 tttgaaaata aagacctaaa gaaactcatt aaaaagaaat ataagcaaga agacgacttt
     4321 gtaaatggtg gaacaagaac agtagagcgt gattataaat acgatgataa aggaaatata
     4381 atagcctacg atgatgggac tgatctagaa tatgaaactg agaaacttga cgaaatcaaa
     4441 tcaaaaattt atggtgttct aagtccgtct aaagatggac actttgaaat tcttggaaag
     4501 ataagtaatg tttctaaaaa tgccaaggta tattatggga ataactataa atctatagaa
     4561 atcaaagcga ccaagtatga tttccactca aaaacgatga catttgatct atacgctaat
     4621 attaatgata ttgtggatgg attagctttt gcaggagata tgagattatt tgttaaagat
     4681 aatgatcaga aaaaagctga aattaaaatt agaatgcctg aaaaaattaa ggaaactaaa
     4741 tcagaatatc cctatgtatc aagttatggg aatgtcatag aattagggga aggagatctt
     4801 tcaaaaaaca aaccagacaa tttaactaaa atggaatctg gtaaaatcta ttctgattca
     4861 gaaaaacaac aatatctgtt aaaggataac atcattctaa gaaaaggcta tgcactaaaa
     4921 gtgactacct ataatcctgg aaaaacggat atgttagaag gaaatggagt ctatagcaag
     4981 gaagatatag caaaaataca aaaggccaat cctaatctaa gagtcctttc agaaacaaca
     5041 atttatgctg atagtagaaa tgttgaagat ggaagaagta cccaagctgt attaatgtcg
     5101 gctttggacg gctttaacat tataaggtat caagtgttta catttaaaat gaacgataaa
     5161 ggggaagcta tcgataaaga cggaaatctt gtgacagatt cttctaaact tgtattattt
     5221 ggtaaggatg ataaagaata cactggagag gataagtcca atgtagaagc tataaaagaa
     5281 gatggctcca tgttatttat tgataccaaa ccagtaaacc tttcaatgga taagaactac
     5341 tttaatccat ctaaatctaa taaaatttat gtacgaaatc cagaatttta tttaagaggt
     5401 aagatttctg ataagggtgg ttttaactgg gaattgagag ttaatgaatc ggttgtagat
     5461 aattatttaa tctacggaga tttacacatt gataacacta gagattttaa tattaagctg
     5521 aatgttaaag acggtgacat catggactgg ggaatgaaag actataaagc aaacggattt
     5581 ccagataagg taacagatat ggatggaaat gtttatcttc aaactggcta tagcaatttg
     5641 aatgctaaag cagttggagt ccactatcag tttttatatg ataatgttaa acccgaagta
     5701 aacattgatc ctaagggaaa tactagtatc gaatatgctg atggaaaatc tgtagtcttt
     5761 aacatcaatg ataaaagaaa taatggattc gatggtgaga ttcaagaaca acatatttat
     5821 ataaatggaa aagaatatac atcatttgat gatattaaac aaataacaga caagacacta
     5881 aacattaaga ttgttgtaaa agattttgca agaaatacaa ccgtaaaaga attcatttta
     5941 aataaagata cgggagaggt aagtgaatta aaacctcata gggtaactgt gaccattcaa
     6001 aatggaaaag aaatgagttc aacgatagtg tcggaagaag attttatttt acctgtttat
     6061 aagggtgaat tagaaaaagg ataccaattt gatggttggg aaatttctgg tttcgaaggt
     6121 aaaaaagacg ctggctatgt tattaatcta tcaaaagata cctttataaa acctgtattc
     6181 aagaaaatag aggagaaaaa ggaggaagaa aataaaccta cttttgatgt atcgaaaaag
     6241 aaagataacc cacaagtaaa ccatagtcaa ttaaatgaaa gtcacagaaa agaggattta
     6301 caaagagaag atcattcaca aaaatctgat tcaactaagg atgttacagc tacagttctt
     6361 gataaaagat attataagaa cggatttaat caaatatgaa aaccaagttg aaactgatga
     6421 aactcgattg acttcagtgc caactgattt gtcaaattat tatttaaaag ttacctctaa
     6481 tgaccataag accaatctac tagcggttca aaaaattgaa gagtcaacaa ttgatggaaa
     6541 acaagtctac aaggtaatag cagtagcgga taacttggtc caacgtaatg tcgataatcg
     6601 ctttgaagaa acttatacct actatattga aaaacccaaa gctagtcagg ctggtatttt
     6661 ctacgacttt gaagaattga tccaagccat tcagacaaat cctgccggtg aatttaaact
     6721 gggtcaaagc atgagcgctc gtaacgttgt tcctaaaggg aaatcttata ttacttcaga
     6781 gtttactggt aaactattga gtgatggtga taaacgctat gctatccata atttggaaca
     6841 tcctttattc gataagatta aggctggaac tgttaaaaat ataaattttg aaaatattga
     6901 tattgattta tctaatcaag ataatatcgc tgcaatagca aatgaaatca gtggaacgac
     6961 acgtatagaa aatatcaaag caatcggaac cataacagct agaaataatg ttgctgggtt
     7021 cgtaaataac ataaaagaag acgctacttt agaaaatgtg gcatttatcg gtagtataaa
     7081 ttctgctggt aataattcaa cagttggagg tattgccggt tctaactata tggggtttgt
     7141 taatagagcc tacgttgatg ctactattac agcaaataac gcaaatgcaa gtatgcttgt
     7201 tcctttcgtt caatataaag ctcatagctg gctatcaggg acaaaaggaa aacttacaaa
     7261 ctctgtagca aaaggtattc ttgatgttaa aaatactaaa tatgttggtg gaataacctc
     7321 taaaacttgg ccatatggag cggttcaaga taatgtaaca tatgcgaaag ttattaaagg
     7381 tcaggaaata ttcggttcaa acgatgttaa tgatggagat gcaaatcctt ttgtgtctaa
     7441 tttatttgga gttattggat atagctcttc tgaagatgga actggtaaag ataataaaaa
     7501 caaaaataaa ttaaaacact tatctaaatc tgaggctgat gcaaaagttg ctagtttcaa
     7561 tattactgct gctaaattcg taagtgaacc ttatgaattg aacaaattga ataatgttag
     7621 cagtcgtgaa gcagcttacg agtctattca agattataat gcagactata aacttgctta
     7681 taagaatatt gaaaaactac aaccattcta taacaaagat tttatcgtta atcaagctaa
     7741 taaactagat aagaacaaca agttaaatac taaagagatt ctatcggtta ctcctatgaa
     7801 caataataac tttgtaactg atttaggtga tgcaaacaaa attatcgttc attatgctga
     7861 tggaacaaaa gactacttca acctatcttc tagtgatgaa ggtctaagta atgttagaga
     7921 atacactata actgacttag gtattaaata tacacctaat atcgttcaaa aagatcatag
     7981 tgctttaatt aacggtatcg taaatatctt aagccctatt gagcttcaat ctaatccaat
     8041 ttaccaaaaa cttggcagaa cagggtctaa tagagtaaat gcaattaaag acctttattt
     8101 agaagaaagt tttgaccatg taaaagctaa tttaaacata ttagtaacta aattagttga
     8161 aaatgaagat catcaactga ataattcatc tgcagctcaa caaatgattc ttgataaagt
     8221 tgagaaaaat aaagctgccc tattattagg tttaacatac ttaaaccgtt actacggtgt
     8281 taaattcggt gatgttaaca ttaaagaatt aatgttattc aaaccagatt tctatggtaa
     8341 aaatgtaagt gtattagata gattaattga aatcggttca agagaagata atataaaagg
     8401 tcatcggaca tatgatgctt tcagcgagat tatttctaag acaaccttat cagaaaattt
     8461 ggtaaacttt ttaaattata atcgtcgact atttactgca gatgtaaata tttatgattg
     8521 gtttaagaaa gctacagaag ggaatgtcta tatagcggag cgtgcttcgg aagtcgatga
     8581 aattaaagcg gcgaaatata aagtttggga taatttacaa tctgctactc atcggaagat
     8641 gatactccca ttattaaatc taaataaatc acatgtgtat ttaatctcga attatagtgc
     8701 aatggctgtt ggaacagcag aaaagttagg gcatagctcc ttcgatggaa taaaaaatca
     8761 gatcgaaaaa gcagcggaca gttatcgaaa ttactttgat ttttggtata gattatcttt
     8821 agataaggtt aagaatatgc tagtcaaaaa tacagtaact cctctttggg aaggttatca
     8881 agcacctggt ggatggcctg ataagtatgg taagaggaat aataaagaag actatgcccc
     8941 attgagagag ttgtttggac ctattggtaa gtattatggt aacaatggaa caggtgctta
     9001 tgcagttatt tgggataatc ctttggatac gcgaacagaa gtaaattata ttgcattaag
     9061 tatgattgat gagtttggaa tctctgttta tacacatgaa actactcatg ttaatgatcg
     9121 tgcaatctat ttaggaggat atggacgtcg ctcagggact catgcagaag cctatgcaca
     9181 aggaatgtta caaacaccag taccaagtac atggtttgat gaatacggtg ctttgggaat
     9241 taatatgact ttctatcgtc caaatgatgg aaatcagtgg tatattaccg atccgaagac
     9301 tcttaaaaca agagaagaca ttgataatta tatgaaaggt tatatagata ccttgtctct
     9361 tcttgattat gttgaaggtg atgcagtcat tagtaaacat gatgataaat taaatcaact
     9421 gtggtttaag aaagttgatc ctattgaacg cactgtaggg tcaaaagatc aatatgacaa
     9481 ggttcgaaat atgagttctg atgattggaa aggaataaca ttaaacagtg ttaatgactt
     9541 agttgataat aatctgatga ctaatcgtat gatgaataat aatatatata agccagaagt
     9601 atttgatcca ggtattgcct atgtaggaat taaaatgatg aatagtatct atgcaggtaa
     9661 cacaagtgag ggtgcacccg gtgctgttac atttaagaat aatgcattta gactctgggg
     9721 atattatggc tatgataaag gattcttagg atatgcttca gataaattaa aatctcaagc
     9781 taataaagaa gggaaacaat ttggagatca atatattata caaaaaatat ctgacggcag
     9841 attccaatcg tttgctgatt ttaagaaaga atattataaa gaattgttag aaaaagccaa
     9901 cgctggtttt actcctttta ctattgatgg tgagaccatt cataactatg atgaattaaa
     9961 atcattattt gccaaagctc ttgaagaaga tatggttact aataaagtta atggtaaact
    10021 tgtgcaagta aaagaaaaag tttataagaa acttcttcaa caaacaaata gctttaaaac
    10081 ttcaatcttt aaataattga aatctctcat ctgctttgcg gatgagggtt ttcttatttt
    10141 atgctatact taaggtatgc atagaaaaac agtgattgat tttaggactt tgggggagag
    10201 atataccttt atccagccta ttaaagagtt gaaaacgaga aatgtagaag aagtggcaga
    10261 tttgctggca caagtggaaa gctaccaaga gcaaggttat tatgtggtgg ggtatgtcag
    10321 ctacgaggct gcacctgctt ttgaggaaaa attagcagtt cacaaggatc ccctactggg
    10381 agagtacctg ctttacttta ctgttcatga tagggtagag acctccccta ttcctctgac
    10441 ttatgaggat attgatttgc cctcaaattg gcaggaagta acgtctgcag agaactatga
    10501 aaaggccatt gcccagatac accatcactt gaggcaggga gacacctatc aggtcaacta
    10561 caccgtccaa ctcaagcaaa agttaagtgc caatcctttt gccatctaca atcgtatggt
    10621 ggtagagcag gaagcgggct acaatgccta tgtggaacat gacgagatgg cagtgatttc
    10681 catgagccca gagctctttt ttgagcaaaa tgatcgcgag ttgacaacac gaccaatgaa
    10741 ggggacgact cagcgtgggg taactgacca agaagatctt gaacaggcca gttggttgga
    10801 acaggatccc aaaaatcgct ctgaaaatat gatgattgtg gatctcttgc gcaatgatat
    10861 gaaccgtatt tctgaagttg ggagcgagca cgtggagcgt ctgtgccagg tagagcagta
    10921 ttcaactgtt tggcagatga cttcgaccat caagagtcag ttgcgagagg atgtggacct
    10981 tgttaaaatc ttccgctcac tctttccttg tggttccata acgggtgcac cgaaaattgc
    11041 gacaatggag attatcaagg acttggagcc tcaaccgcgt ggagtctact gtggaacgat
    11101 tggtctcttg cttccaaatg gacgacggat ttttaatgtg gccattcgta ccattcaact
    11161 tcacaaaggt caagctattt atggagttgg cggagggatt acttgggata gcacatggga
    11221 atctgaatac cgagaggttc atcaaaaggc agcagttctt tatcgtaaac aagctcgctt
    11281 ccaactgatt acgacagggg aaatcagcca gaagaacctg ctgtttgaag ataaacatct
    11341 ggaaagactg agaaaagcta gtcgttattt tgcctttcct tttgatgcag aagacttggg
    11401 acacaagatt gaggaagagt gtcaggattg tgaagctaat caagattacc gcttgcgaat
    11461 cagccttagc aaatctggag agatagaagt caatcgtcaa gtattaaccc ctctcagtac
    11521 aagcttttgt caggcccaag tctgccttca ggaagctgct ttgaatcaat cctttaccta
    11581 ctttaaaacc actcaccgac cgcatttgag cctaggagaa caagagaaga tttaccacaa
    11641 taagtcagga gaactgcttg aaacctctat aggaaatttg gttctgaaaa tcgctggaaa
    11701 actctacaca ccgcctatcc gacttggaat cttgccagga atttaccgtc agtatttgct
    11761 agaaacagga caggtagaag agaaagtctt gaccttggca gatttagccc aagcagaagc
    11821 tatttacggc tgtaatgcag tgagaggctt gtatgaacta agccttgagg agaactagat
    11881 gaaactgata tttttgcatg ggttagggca gtcagcagag tcttggaaag aagttcggaa
    11941 tctactgaca gattatcctt ctgaggctat cgagttattt ccttctggag ttagtaacta
    12001 tcaacaagct aaggagcgag tttatcagta cctagctcaa gagacagaac cttttgtttt
    12061 gattggattg tccttaggtg ctgccctggc actagaactt tccagttacg atttaccaaa
    12121 tcttcgagct ttgattctgt caggatgccc gcttaaatta gcagggaata tcctatttta
    12181 tcttcagttg ctgattttta aactacttcc caaaagagta tttgaaaaac agggagcaga
    12241 caaggctctt atggtcggag tttctgagga gttgaagaca cttgatttaa cggatatagc
    12301 aggaacttgc ccttatccaa ccttactaat ttgtggtatc aaagataaac cgaatcttag
    12361 ttctatgaga agtcttcata aactaatatc agaatcccaa tttcagatta tctcagatgg
    12421 ccctcatgtc ttgaataagg caaaaccaaa ggagtttgta gaaaagacca gaagtttcct
    12481 tgaattgctg aaataagttt gataaaataa tattgaaatc gatatcataa ttatgggaga
    12541 aagaaatgaa taaacgtcta tttttaaaaa tgagtctggt gacgttgcca attttagcct
    12601 tgttttcaca acctgtgtta gcagaggaaa acattcattt ttcgagttgt aaggaagctt
    12661 gggctaatgg atattcggat cttcatgagg gagaacctgg ttattctgcc aagttagacc
    12721 gcgatcatga tggtgtggct tgcgaattga aaaatgctcc taagggtact tttaaagcaa
    12781 aacaagcggc tacaactcaa actgatacaa cttcatccac agcaagtggt tgggttaagc
    12841 aggacggttc ttggtactat tttgatggaa atggaaatct agtgaaaaat gcatggcaag
    12901 gagcttatta ccttaaatca aacggtaaaa tggcacaagg tgagtgggtt tatgattctt
    12961 cttaccaagc atggtattac ttgaaatcag atggttctta tgctcgcaat gcatggcaag
    13021 gagcttatta ccttaaatca aacggtaaaa tggcacaagg tgaatgggtt tatgatgcca
    13081 cctatcaagc ttggtattac ttgaaatcag atggttctta tgcttacagt acatggcaag
    13141 gaaattacta tctaaaatcg gatggtaaaa tggctgtcaa tgaatgggtt gatggtggac
    13201 gttattatgt tggtgctgac ggagtttgga aggaaggtca agcaagtaca gcttcttcta
    13261 gtaatgatag caatagtgaa tattctgctg ctttaggaaa agcaaaaagt tataattcgt
    13321 tattccatat gtcaaaaaaa cgtatgtata gacaattaac ttctgatttt gataaatttt
    13381 caaatgatgc agctcaatat gccattgatc atttagatga ttaacagata ctggtaggcg
    13441 aaaaaattcc ttataattca tttacgcaaa cctttgcata agtgaatgga ttttgatata
    13501 ctagtactgt aagcattttc aattactaca caatgaaaaa ggagaatatg atgagtcaaa
    13561 agattattgg gattgacctt ggtggaactt ctatcaaatt tgcaatctta acaacagcag
    13621 gagaaatcca aggaaaatgg tcaatcaaga ccaacatttt ggatgaggga agtcatatcg
    13681 ttgatgatat gattgagtct attcaacatc gtttggactt gcttggattg gcagcagcgg
    13741 acttccaagg cattggaatg ggatcaccag gtgtggttga ccgtgacaaa gggactgtta
    13801 tcggtgccta caacttgaac tggaaaaccc ttcaaccaat caaacaaaag attgaaaaat
    13861 ctttgggcat tccatttttc atcgataatg atgccaacgt agcagctctt ggtgagcgct
    13921 ggatgggtgc tggtgataac caaccagatg ttgtctttat gacactcggt actggtgttg
    13981 gtggcggtat cgtcgcagaa ggcaaattgc ttcacggtgt tgctggtgca gcaggtgagc
    14041 ttggtcacat cactgttgac tttgaccagc caatctcatg tacttgcggt aagaaaggct
    14101 gtcttgagac agtcgcttct gcaacaggaa ttgtcaactt gactcgtcgc tatgccgatg
    14161 aatacgaagg cgatgcagcc ttgaaacgct tgattgataa cggagaagaa gtaactgcta
    14221 agactgtctt tgatctcgca aaagaaggag acgaccttgc tttgattgtt taccgtaact
    14281 tctcacgtta cttgggaatc gcttgtgcta acattggttc aatcctaaac ccatcaacaa
    14341 tcgtcatcgg tggtggtgtg tcagctgcgg gagaattcct tctacaaggt gttcaaaaag
    14401 tctatgacga aaatactttc ccacaagtac gcacatctac taaattagct cttgcaactc
    14461 taggaaatga cgctggagtt atcggagcag catcacttgt attgcaataa gataatgaga
    14521 gaggaaaatg ctaacctaga gttagtaatg ttcctctttt attttttatg ctatactagt
    14581 taggacaatg aatgaggtga gagtaaatga caaaagcaga tacgattttt aaagagaata
    14641 ttgaacgcat tctaaaggaa ggtgttttct cagagcaggc tcgtcctaag tacaaggatg
    14701 ggactgttgc taattccaag tatgtaactg gtgccttttc cgagtacgac ttatctaaag
    14761 gagaatttcc tatcacaacc ttgcgtccca ttgcaatcaa atccgccatc aaagaagttc
    14821 tctggattta ccaagaccag tcaaatagct tagaagtgct taatgacaag tacaatgttc
    14881 actactggaa tgactgggaa gttggagaca cgggaaccat tggtgagcgc tatggtgccg
    14941 ttgttaagaa acacgacatt atcaataagc ttctcaaaca gttgaaaacc aatccttgga
    15001 accgccgcaa tattatttct ctctgggatt accaagcttt cgaagaaaca gatgggctgc
    15061 tcccgtgcgc ctttcagacc atgtttgatg ttcggcgtgt tgatggggaa atctatctgg
    15121 atgcgacctt gacccagcgc tccaatgata tgctggtggc ccaccacatc aacgctatgc
    15181 agtatgtggc tttgcagatg atgattgcca aacattttgg ctggaaggtt gggaagttct
    15241 tctacttcat caacaacctc catatctatg ataatcaatt tgaacaagct caggaattgc
    15301 tccgtcggga gccgtcaaac tgccaaccac gcttggtttt aaatgttcct gatgggacta
    15361 atttctttga tatcaaagca gaagattttg agttggtgga ttatgaccct gttaagccac
    15421 agttgaagtt tgacctagct atttaaaaga atagaaaaaa gaagttgaga ataatcccaa
    15481 cttcttttgt ttcttaacgt gatacgcggc gacgagctgc ttttttacgg ttttcttcga
    15541 tgaaagctgc tttttgctct tctggttcga ttactttctt tttaaatgcg tatactgcac
    15601 ctgcaacggc agcgacagtt cctgcgacac ctgttacaag acctttagcg aatcctttag
    15661 ccatgagtct tcctccttta tattctcaat cagccagcct cctcaagagg tcacattttt
    15721 ctgactgacc tttttgtgtt ataataatag taacgaaaaa atgggaattt ttcaaggaaa
    15781 aaagatgaaa acaaaaataa ttgtgattgt tggaccgact gctgttggaa agacggctct
    15841 agctattgaa gttgcaaagc gttttaatgg cgaagtggtt agtggagata gccagcaagt
    15901 ctatcgagga cttgatattg ggacggccaa ggctagtcca gaagagcagg cagctgttct
    15961 tcatcattta atcgatgtta gagagataac cgagtcttac tcggcttttg attttgtttc
    16021 agaagctaag atgactattg aggatattca cagccgtggc aagctagcca ttatcgccgg
    16081 tgggactgga ctttatatcc agagcttgct agaaggttac cacctaggtg gggagactcc
    16141 tcatgaggag attttagctt atcgagctag tttggagcca tattcagatg aggaattagc
    16201 ccatttggtg gagcaagcag gccttgagat tccccagttt aatcgtcgtc gtgctatgcg
    16261 tgccttagaa attgcccatt ttggtcagga tttggaaaat caagagacct tgtatgaacc
    16321 gctgattatc tgcttggatg atgaacgtag tcaactttat gagcgtatca accatcgagt
    16381 ggatttgatg tttgaggctg ggcttttgga tgaggccaag tggctgtttg accattcccc
    16441 taatgtacag gctgctaaag gaattggcta taaggaactc tttccttatt tccgtagaga
    16501 gcagaccttg gaggaagctc gtgagagtct caaacaggct actcgtcgtt ttgccaagcg
    16561 tcagctgacc tggttccgta atcgcatgca ggtcaccttt tatcagattg gagaatctgg
    16621 tgtacaagac cgcattttaa gccagataga ggagttttta gatgattgaa acggagaaaa
    16681 aagaggagcg ggtcctgctg attggtgtgg aattgcaggg tatggacagt tttgacctct
    16741 ccatggaaga attggctagt ttagcgaaaa cggcaggggc agtcgttgta gatagctaca
    16801 gacaaaaacg tgaaaaatat gattccaaga ccttcgtcgg ctctggtaag ttggaagaga
    16861 ttgcgcttat ggtggatgca gaagaaatca ctactgtcat cgtcaacaac cgtctgaccc
    16921 caaggcagaa tgtcaatcta gaggaagttc tcggtgttaa ggtcattgac cgtatgcagt
    16981 tgattttgga tatctttgcc atgcgggctc gaagccatga agggaagctc caagtccacc
    17041 tagcccaact caaatacctc ttgcctcgct tggttggtca ggggattatg ctcagccgtc
    17101 aggcaggggg aattggttcc cgtggtcctg gtgaaagcca actggagctg aaccgtcgta
    17161 gcgttcgcaa tcaaatcacg gatatcgagc gccagcttaa ggtggttgag aaaaatcgtg
    17221 cgactgtcag agaaaaacgt ttggagtcta gcacctttaa gattggtttg attggttata
    17281 ccaatgctgg gaaatcaact atcatgaaca tcttgaccag taagacccag tatgaagcag
    17341 atgagctctt tgcgactctg gatgcgacaa ccaagagtat tcatctagga ggcaatctcc
    17401 aagtaacttt gacagatacc gttggcttta tccaagattt gccgacagag ttggtgtcca
    17461 gtttcaagtc aaccttggaa gaaagcaagc atgtggacct tctggttcat gttatcgatg
    17521 ctagcaatcc ttaccacgag gagcatgaaa aaacggttct ctccatcatg aaagacctgg
    17581 acatggaaga tattcctcac ttgacgcttt ataataaagc ggatttggtg gaggatttca
    17641 cgcctactca aacgccatat accctcattt ctgccaagtc tgaggacagt cgtgaaaact
    17701 tgcaagcatt attgctagat aagattaagg aaatttttga agcatttacc ctgcgagtgc
    17761 ctttttcaaa gtcctacaag attcatgatt tagagagtgt tgcaattctg gaagaacgtg
    17821 attatcagga agacggcgaa gtgattacag gctacatttc cgagaaaaat aaatggaggt
    17881 tagaagaatt ttatgactga tattaaaact ttggctctaa agtatggagg ttatacaagt
    17941 ctggacaagg tctatctgga tcagcttcta gctggtaaga cagagcagga gcagttaaca
    18001 cttattacac ctccgccaag tgttgttaat gcctactttg cagaactcta tcagaaaaaa
    18061 agtcctcagg ctgcgacgga ttattttgca gaactcagtc aggaactgaa tctttacaat
    18121 actgagccaa gtttcaccct agaaaataag tcttttattc ggcttaatct gtctggtaaa
    18181 tcctttggtt tttgttatga gagtgacggc tttggtcgga ttttctctga aaatgaggag
    18241 aaaatctcgg atgacttgct ctttgaaatt gcgcaaattt tcccccatca attagtcttt
    18301 gaagagtctg gtaagattta catgaagcct gtcgaggacg aggaagttgt cagcctagaa
    18361 agtctcacag ctttgactga tttggaaaga ttggctgatg gtcgaaaacg tctcaaaggc
    18421 tacagccaag aggatttatt gcaagaagct gctgcttttt ctggcaaacg ctatttccga
    18481 tcggaaaacc gcacagccat gttatatatt gattaattag aaagtatcga atggatattc
    18541 aatttttagg aacgggggct ggtcagccct ctaaagcccg caacgtttca agtctcgccc
    18601 tgaaactttt ggatgagatt aacgaagttt ggctctttga ctgtggagaa ggtacgcaaa
    18661 atcgcattct ggaaaccaca attcgaccac gtaaggtcag caaaatcttt attacccatc
    18721 tgcatggaga ccacattttt ggtttgccag gtttcctttc tagccgtgcc tttcaggcca
    18781 atgaagagca gacagatttg gaaatctacg gacctcaagg aatcaagtca tttgtcttaa
    18841 ccagccttcg tgtgtcaggt tctcgtctgc cctaccgcat tcatttccat gagtttgacc
    18901 aagattctct gggtaaaatt cttgaaaccg ataaattcac tgtgtatgca gaggagctgg
    18961 accacactat tttctgtgtt ggctatcgtg tcatgcaaaa ggatctagaa gggacgctgg
    19021 atgctaaaaa actcaaggct gctggtgttc cgttcggccc gctttttggt aaaatcaaaa
    19081 acggccagga tcttgttttg gaagacggaa ctgaaatcaa ggcagcagac tatatctcag
    19141 cgccacgtcc aggtaagatt atcactattt taggagacac tcgaaaaacg gatgccagtg
    19201 tgcgtctggc tgtcaatgca gatgtcctag ttcatgagtc cacttatggc aagggtgatg
    19261 aaaaaattgc tcgtaaccat ggtcactcaa ctaatatgca agctgcacaa gtagcggtag
    19321 aagcaggtgc caaacgcctc ctactcaacc atatcagtgc ccgtttcctc tcaaaagata
    19381 taagcaaact caagaaagac gctgccacaa tttttgaaaa tgtccatgtg gtcaaagact
    19441 tggaagaagt ggaaatctag cagtcacaga aaggataagt atgcctacta ttctcattac
    19501 cggagctagc ggtggtctag ctcaagaaat ggtcaaactc ctgcccaatg atcaactcat
    19561 cttgcttggt agaaataagg aaaaattagc ccaactctac ggaaattatt cccatgcaga
    19621 attgattgaa attgatatta ccgacgattc agccctagaa gctctggtaa ctgatcttta
    19681 tctccgctat ggcaagattg atgtcttgat taacaacgct ggttacggga tttttgaggg
    19741 atttgaccag attgctgata aagatattca ccagatgttt gaggtcaata cctttgccct
    19801 gatgaatctg tctcgtcacc ttgcggctcg tatgaaggaa agcagcaaag ggcatatcat
    19861 caacatcgtc agcatggcag gtctaatagc tactggcaag tctagtcttt actcagcgac
    19921 caagtttgcg gctattggtt tttcaaatgc tctgcgactc gaacttatgc cctatggagt
    19981 ctatgtgaca acagtcaatc caggtccaat ccgaacagga ttttttgacc aagctgaccc
    20041 agatggaact tatcttaaat cggttgaccg cttcctctta gaggcagatg cagtggctaa
    20101 aaagattgtc aagattatag gcaaaaataa acgagaactc aatctcccga ttttgttgaa
    20161 cctagcccat aagttttata ctctctttcc caagctagct gataagttgg caggggaaac
    20221 ttttaattat aagtaaaaag aaccaatgtg caggttgttg ctagcctaca tattggttct
    20281 tatcatttct caactatcaa actgaacttc ttctagtaga tagtcgataa agcgttcgcc
    20341 catcttagat aggctggttt tctcatgctg gatatagacc agctcaatcg ggtcatcaat
    20401 atccagtggg atagaaacga tattgtctcc gtttaggttg ctgttcaaaa tccctgtcgc
    20461 aatggtataa ccatccaaac caatcaagag attaaagagg gtggcacggt cactgaccac
    20521 aatggatttc ttgtggtgtt cttgagaaag aatctcttct gaaaagtaga aggagttgtg
    20581 cgtcccttgg tcatagctga ggtaagggaa attctccaaa tcagacagtt tcaccttgtc
    20641 tttctttgcc agagggttgg tcttgctgac aaagatatgc ggttgcgctg tgaagagatg
    20701 gtgggctagc aggtgattgt catccagcat cttggttaaa acatcacggt tgtaactgtt
    20761 taagaagagg accccgacct cactgcggaa gttcttgacg tcgtcgataa tctcccaagt
    20821 ccgagtttca cgaaggaaga gttcgtattt ctccatatcg cttttcttga gcaaagagac
    20881 aaaggcattg accacaaagg catagtgttg agacgaaacg ctaaagagtt cgcggtgggc
    20941 gacaggattt ttatagcgtt cctccagaag ctgggtctgc tcgacaacct gacgggcata
    21001 agagagaaac tccatcccat cacgggtcaa ggtgattccc ttgggattgc ggataaagat
    21061 ctcaatgccc atttcatttt ccaaatctcg cactgcattg gagagacttg gctgagtgat
    21121 aaagagttgc ttggctgcct cattcatgga gccagtttcg acgattttga taatataatg
    21181 taattgttga attctcatgt ttttattgta ccatacttgc cttagaatcg ctaaagaaga
    21241 taggaaaata aggagcattg tgtgagtctc ttcttgaaaa aaacacaaaa ttttgctaga
    21301 atgagtctta tgaaaacatt ctatgatgtg cagcaattcc tcaaacgatt tggtattatt
    21361 gtttacatgg gaaaacgatt atatgatatt gaactgatga agttggaact atctcggatt
    21421 tacgatgcag ggttgatgga taaattagac tatctagagg cagaagcagt tcttcgcaga
    21481 gagcacaagg tagaattgga ttatattgag aaaaatggag aaaagaacta atggttactt
    21541 ggattttgtg ggcacttata ctagcaatgt tggcgtggat gggctttaac tatcttcgta
    21601 ttcgccgtgc ggctaaaatt gtggacaatg aggagtttga agccttgatt cgtacgggtc
    21661 aattgattga tttgcgcgac ccagcagaat tccacagaaa acatatcctt ggtgcacgca
    21721 atattccttc aagtcagttg aaaactagtc ttgcagccct tcgtaaagat aaacctgtcc
    21781 ttctctacga aaaccaacgt gcgcaacgag ttacaaatgc agctctttac ttgaaaaaac
    21841 aaggtttttc tgagatttat atcctttctt atggcttgga ttcttggaaa gggaaagtga
    21901 agactagcta accttatttc caaattagtc gaatgatagg atgaaga
//
LOCUS       DBERDK010000030        21070 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.030, whole genome shotgun sequence.
ACCESSION   DBERDK010000030 DBERDK010000000
VERSION     DBERDK010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 21070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21070
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.030"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(198..416)
                     /locus_tag="ACL73L_001128"
     CDS             complement(198..416)
                     /locus_tag="ACL73L_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001207345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGS0772119.1"
                     /translation="MALNYKPLWIQLAKKGLKKTDVIAMAGLTTNVMAQMGKDKPITF
                     KNLERICKALSCTPNDIISFEDNFSDEE"
     gene            complement(662..940)
                     /locus_tag="ACL73L_001129"
     CDS             complement(662..940)
                     /locus_tag="ACL73L_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772120.1"
                     /translation="MKKDKEVYDKVCEYLSPHLNKDMEVFELACWFRVITVIEANSYV
                     NIRSSRLLPKFKTQKINSWCAAYNIHFNNGYCKQHTNEFKDSLKSLIL"
     gene            complement(1154..2776)
                     /gene="rlmD"
                     /locus_tag="ACL73L_001130"
     CDS             complement(1154..2776)
                     /gene="rlmD"
                     /locus_tag="ACL73L_001130"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HGS0772121.1"
                     /translation="MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKILMR
                     VLKVNKKIGFGKVEKYLVQSPHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKDS
                     LYKIAGIADVEVAETLGMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHNLMPLEDFF
                     IQDPVIDQVVVALRDLLRRFDLKPYDEKEQSGLIRNLVVRRGHYSGQIMVVLVTTRPK
                     VFRVDQLIEQVIKQFPEIVSVMQNINDQNTNAIFGKEWRTLYGQDYITDQMLGNDFQI
                     AGPAFYQVNTEMAEKLYQTAIDFAELKKDDVVIDAYSGIGTIGLSVAKHVKEVYGVEL
                     IPEAVENSKKNAQLNNISNAHYVCDTAENAMKNWLKDGIQPTVILVDPPRKGLTESFI
                     KASAQTGADRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVETVALLSK
                     LDVDKHISVEIELDLTSAESKATYAQIKEYVWNKFELKVSTLYIAQIKKKCGIELREH
                     YNKSKKDKQIIPQCTPEKEEAIMDALRHFKMI"
     gene            complement(2850..3824)
                     /locus_tag="ACL73L_001131"
     CDS             complement(2850..3824)
                     /locus_tag="ACL73L_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HGS0772122.1"
                     /translation="MRKKLFLTSAAILWAVTAMNSVHAATDVQKVIDETYVQPEYVLG
                     SSLSEDQKNQTLKKLGYNASTDTKELKTMTPDVYSKIMNVANDSSLQLYSSAKIQKLG
                     DKSPLEVKIETPENITKVTQDMYRNAAVTLGMEHAKITVAAPIPVTGESALAGIYYSL
                     EANGAKVPQANKDLAQEELKALSDINAENKDKSGYDANKLNVALADIKSGLAKAKESK
                     GNLTEEDVRKIVEDTLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKTLNDL
                     KQSIVSQAGDSFKNINLNFDADKALEDGGNFLSSLWQALVNFFKSFGS"
     gene            complement(3898..4512)
                     /gene="pmp23"
                     /locus_tag="ACL73L_001132"
     CDS             complement(3898..4512)
                     /gene="pmp23"
                     /locus_tag="ACL73L_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HGS0772123.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     QDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGIQTLTGNLY
                     LAQKKGVDIWTAVQAYNFGPAYIDFIAQNGKENTLALAKQYSRETVAPLLGNRTGKTY
                     SYIHPISIFHGAELYVNGGNYYYSRQVRLNLYIIKCFTLFSTSG"
     gene            complement(4512..5486)
                     /locus_tag="ACL73L_001133"
     CDS             complement(4512..5486)
                     /locus_tag="ACL73L_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HGS0772124.1"
                     /translation="MDIYIKKAIIHQFSPDDTELFLADKFLNITPKIEEYLRKKIEHV
                     YSDEAKTGIFEEENPFFNHITDDLLETSVTLANLWKEEFSISENLKTNDLIFVQFSKE
                     GVEHFAFLRIALRETLTHLGGEVDNPIKLTQNNLPGFGTGADEALVVNLQSRKYHLIE
                     KRIKYNGTFLNYFSDNLLAVAPKISPKKSIKELEKTAQRIAESFNTDDFQFQSKVKSA
                     IFNNLEESNELSPEKLANDLFDNNLTARLSFIDQVKEAVPEPVQFDEIDASRQLKKFE
                     NQKLSLSNGIELIVPNNVYQDAESVEFIQNENGTYSILIKNIEDIQSK"
     gene            complement(5494..6750)
                     /gene="glyA"
                     /locus_tag="ACL73L_001134"
     CDS             complement(5494..6750)
                     /gene="glyA"
                     /locus_tag="ACL73L_001134"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HGS0772125.1"
                     /translation="MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMSLIEPGDTVMGMDLASGGHLTHGAPVSFSGQTYNFVSYSVDPETELLDFDAIL
                     KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPV
                     PYAHITTTTTHKTLRGPRGGLILTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFK
                     EVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQ
                     NLLDEVNITLNKNSIPYETLSPFKTSGIRIGAAAITARGFGEEESRKVAALIIKTLKN
                     SENEAVLEEVRSAVKELTDAFPLYED"
     gene            complement(6812..7243)
                     /locus_tag="ACL73L_001135"
     CDS             complement(6812..7243)
                     /locus_tag="ACL73L_001135"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014947335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS0772126.1"
                     /translation="MLRDLQETDVKAICDINQEALGYTFSPEETASQLARLSQDSHHF
                     LLGYEDAANHVLLGYVHAEVYESLYSKAGFNILALAVSPQAQGQGIGKSLLQGLEEEA
                     KRCGYGFIRLNSANHRLGAHAFYEKVGYTCDKMQKRFIRIC"
     gene            complement(7245..7844)
                     /locus_tag="ACL73L_001136"
     CDS             complement(7245..7844)
                     /locus_tag="ACL73L_001136"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HGS0772127.1"
                     /translation="MDRIRQELENGGAVVLPTETVYGLFAKALDEKAVDHVYQLKRRP
                     RDKALNLNIASFEDILHFSKNQPAYLQKLVETFLPGPLTIILEANDRVPYWVNSDLAT
                     IGFRMPSHPITLDLIRETGPLIGPSANISGQASGVTFEQILKDFDQEVLGLEDDAFLT
                     GQDSTIVDLSGDKVKILRQGAIKREDILARLPEISFEEA"
     gene            complement(7831..8670)
                     /gene="prmC"
                     /locus_tag="ACL73L_001137"
     CDS             complement(7831..8670)
                     /gene="prmC"
                     /locus_tag="ACL73L_001137"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HGS0772128.1"
                     /translation="MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQ
                     QEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVEL
                     ILTENLETNLSVLDIGTGSGAIALALAKNRPAWSVTAADVSQEALELASENASNQNLN
                     IFFKKSDCFAEISEKYDIIVSNPPYISREDESEVGLNVLHSEPHLALFADEDGLAIYC
                     RIAEDAKDYLKDGGKIYLEIGYKQGQSVPELFRKHLPEKRVRTLKDQFGQNRMVVVDD
                     GQD"
     gene            complement(8670..9749)
                     /gene="prfA"
                     /locus_tag="ACL73L_001138"
     CDS             complement(8670..9749)
                     /gene="prfA"
                     /locus_tag="ACL73L_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HGS0772129.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEASNR
                     DTVIAYREYKQVLQNIVDAEEMIKESGGDADLEELAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLEELNK"
     gene            complement(9746..10336)
                     /locus_tag="ACL73L_001139"
     CDS             complement(9746..10336)
                     /locus_tag="ACL73L_001139"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HGS0772130.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GVGYVSSRIGMKRPALAIEETTDIFGYIRDLPEKPYCVLVDEAQFLKRHHVYDLARVV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFAPEINKENEEK"
     gene            10459..10641
                     /locus_tag="ACL73L_001140"
     CDS             10459..10641
                     /locus_tag="ACL73L_001140"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HGS0772131.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAVVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            10777..12150
                     /gene="mnmE"
                     /locus_tag="ACL73L_001141"
     CDS             10777..12150
                     /gene="mnmE"
                     /locus_tag="ACL73L_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HGS0772132.1"
                     /translation="MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIAQKIFKGKDL
                     SQVASHTLNYGHIIDPLTGKVMDEVMVGAMKSPKTFTREDIIEINTHGGIAVTNEILQ
                     LAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNIRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTKLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEADLVLLVLNASEPLTAQDRQLLEISQDTNRII
                     LLNKTDLPETIETSKLPEDVIRISVLKNQNIDKIEERINNLFFENAGLVEQDATYLSN
                     ARHISLIEKAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLF
                     SQFCLGK"
     gene            complement(12197..13132)
                     /gene="dapA"
                     /locus_tag="ACL73L_001142"
     CDS             complement(12197..13132)
                     /gene="dapA"
                     /locus_tag="ACL73L_001142"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HGS0772133.1"
                     /translation="MSYQDLKECKIITAFITPFHEDGSINFDAIPALIEHLLAHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKVVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKAIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     HPNIIGVKECTSLANMAYLIEHKPEEFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFTAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAILNYMGFEAGPTRLPL
                     VPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY"
     gene            complement(13188..14264)
                     /locus_tag="ACL73L_001143"
     CDS             complement(13188..14264)
                     /locus_tag="ACL73L_001143"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HGS0772134.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEESTLPIDKIRYLASARSAGKS
                     LKFKDQDITIEETTETAFEGVDIALFSAGSSTSAKYAPYAVKAGVVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGVKPCDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIAAF
                     PGAVLEDDVAHQIYPQAINAVGSRDTFVGRIRKDLDAEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            complement(14607..14679)
                     /locus_tag="ACL73L_001144"
     tRNA            complement(14607..14679)
                     /locus_tag="ACL73L_001144"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14644..14646),aa:Thr,seq:ggt)
     gene            complement(14779..15288)
                     /locus_tag="ACL73L_001145"
     CDS             complement(14779..15288)
                     /locus_tag="ACL73L_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HGS0772135.1"
                     /translation="MKKLTIRDVADIAIVAAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLLDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIRKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     regulatory      complement(15286..15382)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            15411..15842
                     /locus_tag="ACL73L_001146"
     CDS             15411..15842
                     /locus_tag="ACL73L_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HGS0772136.1"
                     /translation="MKIPIQKFFNNEILAYLFFGIVTTLVAILLRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLFLTFLFVTQFP
                     HIIGQFVTENIDKINAIETIIAQIFIIIINYILSKVYIFRK"
     gene            15924..16301
                     /gene="mscL"
                     /locus_tag="ACL73L_001147"
     CDS             15924..16301
                     /gene="mscL"
                     /locus_tag="ACL73L_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HGS0772137.1"
                     /translation="MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKEE
                     KTAEKKPTELEVLQEIKALLEKK"
     gene            complement(16343..17437)
                     /gene="hemH"
                     /locus_tag="ACL73L_001148"
     CDS             complement(16343..17437)
                     /gene="hemH"
                     /locus_tag="ACL73L_001148"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HGS0772138.1"
                     /translation="MKKAILMMTFGSPEEITFEGVADFFTNIRRGMRPQDHEIQTLYD
                     NYVRIGGTPLQKITRQEVALVEARLGNEYSVYFANKFSSPFIPDVIGQMEADGIEQCI
                     CLILEPHYSFYSVMGYEKFLESKQIQFLVIKDWYQEEALLNYWADEIAKILKEEVKQD
                     SFKVIFSAHSVPIFALDFGDPYIDQIFENSKLVAEKLGLSSEQYTNTWQSESDIGIPW
                     IKPDVLEYLREQTEHPDHYIFVPISFISEHIEVLFDNDVECYDLCQEFGVNYHRPPMP
                     NTDSRLIDALVNTVRVNENQEFKEFLPEEETFDELVPSDETKNILAESQDLQMPEFVK
                     KLIEKKGRENVKMPYLIKKMLEKAGKLPKE"
     gene            17592..18815
                     /gene="pepT"
                     /locus_tag="ACL73L_001149"
     CDS             17592..18815
                     /gene="pepT"
                     /locus_tag="ACL73L_001149"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HGS0772139.1"
                     /translation="MTYPNLLDHFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPE
                     MKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDG
                     GVIELGNSGFKLDPADFKSLEKYPGQTLITTDGTTLLGADDKSGIAEIMTAIEYLTAH
                     PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAEL
                     HFQGRNVHPGTAKGQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEV
                     RASYIIRDFEKDAFEARKASMQSIADKMNEELGSDRVTLNLTDQYYNMKEVIEKDMTP
                     ITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTM
                     ERAVDTIIGIVAYKG"
     gene            complement(18851..19504)
                     /locus_tag="ACL73L_001150"
                     /pseudo
     CDS             complement(18851..19504)
                     /locus_tag="ACL73L_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672614.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family hydrolase"
     gene            19502..19666
                     /locus_tag="ACL73L_001151"
                     /pseudo
     CDS             19502..19666
                     /locus_tag="ACL73L_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057566.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(19743..20386)
                     /locus_tag="ACL73L_001152"
                     /pseudo
     CDS             complement(19743..20386)
                     /locus_tag="ACL73L_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672611.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            complement(20493..21053)
                     /locus_tag="ACL73L_001153"
                     /pseudo
     CDS             complement(20493..21053)
                     /locus_tag="ACL73L_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672571.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
BASE COUNT         6085 a         4387 c         3926 g         6672 t
ORIGIN      
        1 ttcaaggatt attgctacat cattcatatt tttaggtaaa taccaaccat ctggcttatc
       61 tgaatatccc ttgaagccta attgattaaa agtagttatt tgaccagtac cttgattgat
      121 gttttcttca acttcattaa agcctatact tctcttgcat aatccctaac ttgatcttct
      181 gtccttaaag tcattttcta ttcctcgtca ctaaaattat cttcaaaact aataatatca
      241 ttaggagtgc aagataaagc cttacatatt ctttctaaat tcttaaatgt aattggttta
      301 tcctttccca tttgtgccat aacatttgtt gtaagtcctg ccatagctat tacatctgtt
      361 ttctttagtc ctttttttgc taactgtatc cataatggtt tatagttaag tgccataata
      421 tccctctttt tttcttcatt agatttattt aattatagca taaatattga tttcattcaa
      481 tcttacattg atttagtgat ttgttttaga gaatttgttg tgttattccg tccttatttt
      541 gccataaatt gttttaagtg ggacctctta tccttaccat ttttacctcc taattttgag
      601 tataaaaaag acgatagagt ttttaatttc tatcgtctga gatttacata ttaaattttc
      661 atcacaaaat aagactcttt aaagaatctt taaattcatt ggtatgctgc ttacaatatc
      721 cattattaaa atggatattg taagcagcac accaactatt tattttttga gttttaaact
      781 ttggtagaag tcttgaactc cttatattta cataactatt tgcctctata actgttatga
      841 cacgaaacca acaagcaagt tcaaacacct ccatatcttt attcaaatga ggactaagat
      901 attcacaaac tttatcataa acctctttat cttttttcat aaaaggggca tacaacttag
      961 caaatttatc ctaaaatttt ttgttgcctt tatcaatcat ttcaacgctc ctttaaatat
     1021 aaaaatgatc ctatcaatct ttatattgat cctattttct aattgatacc ttgcaagaaa
     1081 ggaataatag ctgttcctat accaagtagg aatgtaataa atgcagaaag tcatatactg
     1141 tcattctttt ctattaaatc attttgaagt gtctcaaagc atccatgatg gcttcttctt
     1201 tttcaggtgt acactgtgga ataatttgtt tatccttttt agacttgttg taatgttctc
     1261 gtaattctat tccacatttc ttttttatct gtgcaatata taatgtcgaa acttttaatt
     1321 caaatttatt ccaaacatat tctttgattt gagcatatgt tgctttgctc tccgcacttg
     1381 tcaaatccag ctcaatttca acacttatgt gcttatcgac atcgagtttg gacaaaagtg
     1441 ctaccgtctc gacgtgatgc gtttgaggaa atagatccac cggctggact ttcttcaatt
     1501 catatcccaa ctcttggtat agtttaatat cacgcgccat ggttgcgaca ttgcaggaga
     1561 tataggcgat gcgatcggct cctgtttggg cgcttgcttt gataaagctt tctgtcaagc
     1621 ccttgcgtgg aggatcaacc aagataacgg ttggttgaat cccatcttta agccaattct
     1681 tcatagcatt ttcagctgtg tcacagacat agtgggcgtt tgaaatattg ttcagctgag
     1741 catttttttt actattctca accgcttctg gaatcagttc aacaccgtag acttctttga
     1801 catgcttggc gactgataaa ccaatggttc caataccaga ataagcatca ataaccacgt
     1861 catctttttt taactctgca aagtcaatgg ctgtttgata gagtttctcc gccatttcag
     1921 tattgacttg gtaaaaggct gggccagcga tttggaagtc atttcccaac atctggtccg
     1981 taatatagtc ttgaccataa agagtgcgcc actccttacc aaaaatcgca ttggtattct
     2041 ggtcgttgat attttgcatg acagacacaa tctctgggaa ctgcttgata acttgttcaa
     2101 tcaattggtc aacacgaaaa acttttggac gagttgtcac caaaacgacc atgatttgtc
     2161 ctgaatagtg accacgacgc accacaagat tccgaatcaa tccagactgt tccttttcgt
     2221 cataaggttt taaatcaaaa cgacggagca ggtctcgaag agctactacg acttggtcaa
     2281 tgacaggatc ctggataaag aaatcttcaa ggggcatgag gttgtgcgaa ttcttacgga
     2341 aaaatcctgt ttccaagaca ccattcactc gacgaacggg cacctgcgcc ttattgcgat
     2401 acttgactgg atgttccata ccaagcgttt cagcaacttc tacatctgca attccagcaa
     2461 tcttgtagag actgtccttg acttgcttgg ttttaaactt gagctgttct ggataagaaa
     2521 ggtgtcctaa atccgcgatt cctgaacgca ggtaagccaa atctagatct tgattacggt
     2581 gtggtgactg gacaaggtat ttttcaactt ttccaaagcc aatcttttta ttgaccttga
     2641 ggacacgcat gagaattttt tcactcggta aagcattctc tacaaaaaag accaaaccat
     2701 ctaccttggc aactcctgcc ccttcatggg tcaaatcaac aatttcaact tctacaatat
     2761 catttttctt taacattctt ttctctttct attggccgac aaaaaagacg gcaacgttac
     2821 ggttaccatc cattatacca tgaaatttct taagaaccaa aactcttgaa gaagttgaca
     2881 agggcttgcc agagggagct taagaagtta ccaccgtcct ctagcgcctt atccgcatca
     2941 aagttaaggt tgatattttt aaaactgtcg ccagcttgtg atacgatgct ttgtttaagg
     3001 tcatttaggg ttttagtgaa atctgcattg ctgaggatat cactctttga gagattcaag
     3061 gcaaaattga tgatgatatt gatctggttt cctgttatga cctgatcaag tttgtaattt
     3121 tttaaggtat cttcaacaat cttgcggaca tcttcttctg tcagatttcc cttgctttct
     3181 ttagctttgg caagtcctga cttgatatca gctagggcaa cgtttaattt attagcatca
     3241 tagcctgatt tgtccttgtt ttcagcattg atatctgaca aagccttcaa ctcttcttga
     3301 gccaaatctt tattagcttg tggcaccttg gctccattag cctctagcga atagtaaatc
     3361 cctgctaagg cactttctcc tgtaaccgga ataggggctg ctacagtgat tttggcatgt
     3421 tccataccca gcgttactgc tgcgtttcgg tacatatcct gagtcacctt agtgatattt
     3481 tctggtgttt caatcttgac ctcaagtggc gatttgtcac ctagcttttg aatcttggct
     3541 gatgaataca actgtaagct agagtcattg gccacattca tgattttaga ataaacatca
     3601 ggtgtcatgg tcttgagttc tttggtatct gttgaggcat tgtagcccag ttttttaaga
     3661 gtttgatttt tttggtcttc agatagggaa gaacctagga catattcagg ttggacatag
     3721 gtttcatcga taactttttg aacatctgtt gctgcatgaa cgctattcat agctgttact
     3781 gcccacaaga tcgcagcgct agtcagaaag agtttctttc tcatagggaa ttgcctcctt
     3841 tacttcttta gagtaatata tctatcttaa agaaaactta taacaaaaac acctggtcta
     3901 gccagatgtt gaaaagagag tgaaacattt gatgatgtaa aggttaagtc gtacctgtct
     3961 agaataataa tagtttcctc catttacata gagttcagca ccgtgaaaaa tggaaatggg
     4021 gtgaatataa ctataagtct ttccagtcct attaccaagc aagggggcaa cagtctcacg
     4081 agagtactgt ttggctagag ccagggtatt ttccttgcca ttttgggcga taaaatcgat
     4141 ataggcaggt ccaaaattat aggcttgaac agctgtccag atatctacac ccttcttctg
     4201 cgccagatag agattgcctg tcagagtttg aatgccttgc cgaatgctag aggcattatc
     4261 attgatggtg ttggtggaac cacttgcaga ctcactagac tgcataacat cgccttcttt
     4321 tccttttgtt tcagtataaa tcatagcaag cacaagctct tcgtttgctg gggtgtcttg
     4381 ttcactcaat atttctcgca ccatgggttg ataggtcatg acttgtttga catcttgatg
     4441 aacgcggtaa gctttatagc cagcaaaaag gaagactgct agtacaagca ctcttcgaat
     4501 tcgtttaaac attatttact ttggatatcc tcgatatttt tgattaagat agagtaggtt
     4561 ccattttcgt tttggataaa ctcaacagac tcggcgtctt gatagacgtt attgggaacg
     4621 atgagctcaa ttccatttga taaggagagt ttttggtttt caaatttctt taattggcga
     4681 ctggcatcaa tttcatcaaa ttgaacaggt tctggtacgg cttctttgac ttggtcaata
     4741 aagctcaaac gagccgtcag attgttgtca aaaaggtcat tagccaattt ctcaggtgac
     4801 aattcattgc tttcttctag gttgttgaaa atagctgatt tgaccttgga ttgaaattga
     4861 aaatcatctg tgttaaaaga ttcagcaatt ctctgggctg ttttttccag ttccttgata
     4921 gattttttag gagaaatctt aggagcgaca gcaagaagat tatctgaaaa atagttcaaa
     4981 aaagtcccgt tgtacttgat tcgtttttca atcaggtgat acttgcgact ctgaagattg
     5041 accaccaagg cctcgtcagc acccgttcca aatccaggca ggttattctg agtcagcttg
     5101 attggattat caacttctcc tccgaggtgg gtcaaggtct cccgcagggc aattcgcaag
     5161 aaagcgaaat gttctacacc ttctttagaa aattgaacaa aaatcaagtc attggtcttg
     5221 agattttcag aaatgctaaa ctcctctttc caaagattag ccagcgttac tgatgtctcc
     5281 aacaaatcgt ctgtaatatg attgaagaag ggattttctt cttcgaaaat cccagtcttg
     5341 gcttcatctg aatacacatg ttcaattttt ttacgtaggt attcttcgat ttttggagta
     5401 atattgagaa acttatctgc taagaacagc tcggtatcat ccggactgaa ctggtgaata
     5461 atggctttct taatataaat gtccataaaa gttttagtcc tcgtataatg ggaaggcatc
     5521 tgtcaattct ttgactgcac ttctcacttc ttctaataca gcctcatttt ctgaattctt
     5581 aagggtttta atgatgagtg cagccacttt gcgactttct tcttcaccaa atccacgtgc
     5641 agtaatagct gctgctccga tacgaatccc acttgtcttg aatggtgaca aggtttcgta
     5701 agggattgaa tttttattta gggtaatatt gacttcatcc agcaagtttt gagcaacttt
     5761 tccgttttct acaaccttag tcacatcaac aaggaagaga tggttttcag ttccacctga
     5821 aataatacgg aaatcagggt cttgcaagaa gacatctgcc atagccttgc tgttcttaat
     5881 tacattggca gcatattcct tgaaggctgg atccaaaact tctttgaagg aaactgcctt
     5941 agccgccaca acatgctcta aaggaccgcc ctgaatacct gggaaaatag ctgaattgat
     6001 ttttttagca agttcttcgt cattggtcaa aatcaaacca ccacgaggtc cacgaagggt
     6061 tttgtgggtc gttgttgttg tgatatgagc gtatggaact gggcttggat gaaggccagc
     6121 cgcaaccaaa ccagcgatat gggccatgtc caccatgagc ttcgcaccga cagcatctgc
     6181 gatttcacgg aattttgaaa aatcgataat ttgagaatat gctgaagcac cagctacaat
     6241 cagttttggt tttacttctt gggcttgttt caagatagca tcaaagtcta agagttctgt
     6301 ttcaggatcc acactataag aaacaaagtt gtaggtttga ccagagaagc taacaggagc
     6361 cccatgagtc aaatgaccac ctgatgccaa atccattccc ataaccgtat cacctggctc
     6421 aatcaaggac atgtaagccg cacagttagc ttggcttcct gaatgtggtt ggacattggc
     6481 aaatttagca ccgaaaattt cttttgcgcg ttcaatagca agagtctcta caacgtctac
     6541 tacatcagtt ccaccataat aacggcgtcc tgggtaaccc tcggcatatt tatttgtcaa
     6601 gatagaccct tgagctgcca taacagcctt ggaaactacg ttttccgaag caattaactc
     6661 gatattattt tgttggcgtt cttcttcttt ggcaatagca ttccagagat cagcatcata
     6721 tgctttaaaa tcatctttgt caaaaatcat aggtcttctc ctttattgtg tgactagtcc
     6781 attagtttga ttttacaata agaaaatcaa actaacagat gcgaataaac cgtttctgca
     6841 ttttatcaca agtatagcca actttttcat aaaatgcatg agcacccaga cgatgattgg
     6901 cagaatttaa gcggataaac ccataaccac atctttttgc ttcttcttcc aacccttgta
     6961 gtaaactttt accaatacct tgaccttgcg cttgaggtga aactgctaaa gctaagatat
     7021 taaatcctgc tttggaatag agtgattcgt aaacttcagc gtggacatat ccaagtaaga
     7081 catgattagc tgcatcctca tagccaagta ggaaatgatg ggaatcctga gacagtctag
     7141 ctagttggct agccgtttcc tctggactaa aagtataacc caaagcctct tggttgatgt
     7201 cacatatcgc tttcacatct gtttcttgca aatctcttag catttcaagc ctcctcaaaa
     7261 gaaatctctg gcaaccgagc aagaatatct tctcgtttaa ttgcgccttg gcgtaagatt
     7321 ttcaccttgt ctccagacaa atccacaata gttgaatcct gtccagttag aaaagcatcg
     7381 tcttccagac ccagaacctc ttggtcaaaa tccttcagaa tttgttcaaa ggttacacca
     7441 cttgcctgac ctgagatatt ggcagacggc ccaatcaagg gacctgtctc tcgaattaaa
     7501 tccagtgtga tagggtgact gggcatccga aatccaatag ttgcaaggtc agaatttacc
     7561 caatagggaa ctcggtcatt ggcttcgaga ataatggtca agggacctgg caaaaaggtc
     7621 tctacaagtt tttgtagata agctggctga ttcttagaaa agtgcaagat gtcctcgaaa
     7681 gaggcgatat tgagattgag tgccttatct ctaggacgac gtttgagttg gtaaacatgg
     7741 tcgacagctt tttcatctaa ggccttagca aaaagaccgt aaaccgtctc tgtaggtaga
     7801 acgacagctc caccattttc caactcttgt ctaatcctgt ccatcatcaa ccacaaccat
     7861 cctgttttga ccaaattggt ccttgagtgt tcgaactctt ttttcaggaa gatgtttcct
     7921 aaaaagttcg ggaacacttt gaccttgctt gtatccaatt tcaaggtaaa tcttaccacc
     7981 atctttgaga tagtcttttg catcttccgc aattctacag taaatagcta ggccatcctc
     8041 gtctgcaaag agagctagat gaggctccga atgcaaaaca ttcaagccta cctctgactc
     8101 atcttcacga gagatatagg gtggattgga tacaattata tcatattttt cagaaatttc
     8161 tgcaaaacaa tcagattttt taaaaaatat attaagattt tgattgctag cattctctga
     8221 tgcaagctct aaggcctctt gagaaacatc tgctgctgtc actgaccaag ctggtctatt
     8281 ttttgctaga gcgagagcaa tagctccact acctgttcca atatccagaa ctgagagatt
     8341 cgtctcaaga ttttcagtta gaataagctc cactaactcc tctgtttctg gacgaggaat
     8401 caaaacccgt tcatccacct ttaaatgcat tccataaaaa tctgcctgtc caatgatgta
     8461 ctgagctggc ttgtgagctg ctagttgctg gtaaatatct tctacaaatt gtttttcttc
     8521 ctctgttgtc acctcctgct ggagggcaaa aataaagtct gtaaaagata gatttttcag
     8581 actacgatag acaaaagaga ggctttccgc ttcctctcct tgtcttatca actcttcttc
     8641 aaaatttgaa aataattgag ctaatttcat tatttgttta attcttctag tttttgtgtt
     8701 tggtcataaa gcaccaaggc atccacaact tcgtccaatt taccagacaa aatcgtatct
     8761 agtttttgga gggtcaagcc gatacggtgg tctgtgacac ggttttgtgg gaagttataa
     8821 gttcggatcc gttctgaacg gtcaccagta ccgattgtcg acttacgctc agcgtcctgc
     8881 tcatcttgag caatctgagc aaagtggtca gcaacacggg cacggatgat tttcatggcc
     8941 ttctcacggt ttttctgctg ggtacgttct tcttgcatct caaccttgat attggttggc
     9001 aagtgaacga tacgcacggc agtcgcaacc ttattgacgt tctgaccacc agcacctgat
     9061 gcgtggtaaa tatcaacacg aagatctttt ggatcgatat cgtattctac ttcttcgact
     9121 tcaggcatga caagaactgt ggcagttgag gtatgaacac gaccttggct ttctgtcacg
     9181 gggacacgct gcacacggtg ggcacctgat tcatacttaa gcttagagta tacagactga
     9241 cctgaaacca tagcaaccac ttctttaaaa ccaccgacac cattcataga ggcttccatg
     9301 acttcaaagc gccaaccttg ggcttccgca tacttttggt acatagttag caaatctcca
     9361 gcgaaaagtg ccgcttcgtc tccaccagct gctccacgga tttcaaggat gatattcttg
     9421 tcatcgtttg gatcctttgg aaggagcaaa attttcagtt tttcttcata ttcttctttt
     9481 tcagccttgg catctttgag ttcttgcttg gccaattctt ccaagtccgc atctccgcct
     9541 gattccttaa tcatctcttc ggcatcgacg atattttgaa ggacttgttt atactcacgg
     9601 taggctatta cggtgtcacg attggaagct tcttcttttg aaagctccat aaaacgcttg
     9661 gtgtctgaaa cgacatcagg gtcactcagc aattctccta gttcttcata acggtcttct
     9721 acagcttgta gttgatcata gatgttcatt tttcttcatt ctccttattg atttcagggg
     9781 caaaataatg tttacggcaa accgagatat aggtttcatt accaccaatc tggatctgtt
     9841 ctccatcgta aacgggcagt ccatcctgtg ttcgcaacac catggtcgcc tttttcttgc
     9901 aatactgaca gatggtcttg atttcgtcaa tcttgtctgc taaaagcaag agatatttgg
     9961 aaccttcgaa caattcattg cgaaagtcat ttttcaagcc aaaagccatg acgggtatgt
    10021 ctaactcgtc cacaacacga gctaggtcat aaacatggtg gcgtttgaga aactgggctt
    10081 catcgaccaa aacacagtaa ggtttttctg gtaggtctcg gatatagcca aagatatccg
    10141 ttgtttcctc aatagcaagg gcagggcgtt tcatgccaat tcgactcgac acatagccaa
    10201 caccgtcacg cgtatccaga gccgaggtca taatcacaac accttttcct tgctcctcgt
    10261 agttataggc cactttgaga atctcaatcg ttttaccaga gttcatggtc ccataacgat
    10321 agtacaactg tgccatgttt cttgcttcac gtccatttct aaatttttgc tacattctag
    10381 tatatcataa ttttcttaag ctttaaacgg caaaatgtgg taaaatggaa gaaatcaaaa
    10441 ctagtggagg aagctattat gccatttgta cgcatcgatt tatttgaagg acgcacgctc
    10501 gagcaaaaga aagctcttgc taaggaagta acggaagcag ttgtccgcaa cactggagca
    10561 cctcaatctg ctgtccatgt catcatcaac gacatgccag aaggaactta cttcccacaa
    10621 ggggaaatgc gtactaaata agctagctta agcagaattg cttaggcttt ttcaatctcc
    10681 aagtagcacc cattgaagaa atagtctaaa tttgttacaa tttgaaaaga gacttggaaa
    10741 attccaagaa aagagcaatt aattaaagga aacattatga ttacacgtga atttgatacc
    10801 atcgctgcta tctctactcc actaggtgaa ggagctattg gtattgtccg cctgagcgga
    10861 acagacagtt ttgctattgc gcaaaagatt tttaaaggaa aagacttgag tcaggttgct
    10921 agccacactc tcaactacgg tcacattatt gatcctctga ctggtaaagt catggacgag
    10981 gttatggttg gggctatgaa gtctccaaag accttcactc gtgaggatat tatcgagatt
    11041 aacacccacg gtggtattgc ggtgaccaat gagattctcc agctagctat ccgtgaagga
    11101 gctcggttgg cagaacctgg tgaatttacc aaacgtgcct ttttaaacgg tcgcgtagat
    11161 ttgacacagg ccgaggcggt gatggatatc atccgagcca agacagacaa ggccatgaac
    11221 atcgcggtca aacaacttga tggttccctt tctgatctta tcaataatat ccgtcaagaa
    11281 atccttaaca cacttgccca agttgaggtc aatatcgact atcctgagta tgacgatgtt
    11341 gaggaagcca ctactgctgt tgtccgagag aagacaatgg agtttgagca attactaacc
    11401 aaactcctta ggacagcacg tcgtggtaaa atccttcgtg aaggaatttc aacggctatc
    11461 attggacgtc ccaacgttgg gaaatcaagc cttctcaaca acctcttgcg tgaggacaag
    11521 gctatcgtaa cagatatcgc tgggacaaca cgagatgtca tcgaagagta cgtcaacatc
    11581 aatggtgtac ctctcaaatt gattgataca gccggtattc gtgaaacaga tgacatagtg
    11641 gaacaaatcg gtgttgagcg ttcgaaaaaa gccctcaagg aagccgactt ggttctacta
    11701 gtgctaaatg ccagtgaacc actgactgcg caagacagac aacttcttga aattagccaa
    11761 gataccaatc gcattattct acttaataaa accgacctgc cagaaacgat tgaaacttcg
    11821 aaactacctg aagacgttat ccgtatttca gtccttaaaa accaaaacat cgacaagatt
    11881 gaagagcgaa tcaacaacct cttctttgaa aatgctggct tggtcgagca agatgctact
    11941 tacttgtcaa acgcccgtca catttccctg attgaaaaag cagttgaaag cctacaagcc
    12001 gttaatcaag gtcttgagct ggggatgcca gttgatttgc ttcaagttga cttgactcgt
    12061 acttgggaaa tcctcggaga aatcactggg gatgctgctc cagatgaact catcacccaa
    12121 ctctttagcc aattctgttt aggaaaataa gaaaaatcca tgatccttca ttcggtcatg
    12181 gattttattg tctttattag taatctggtc ttaagacccc tgttacagtt gccttagttg
    12241 cttcgtagtc gccatctacg acaaccttga taatgcgttt ggcatcttct tctggtgctg
    12301 gaacaagagg tagacgagtg ggtccagctt caaatcccat atagttaaga attgccttaa
    12361 ctggagcagg acttggataa gagaagagag cattaacctt aggaatgaat ttacgctgaa
    12421 ttgctgcggc tttcttcata tcgctttctg caatggcagt aaacatctcg tgcatttcat
    12481 ccccatttgt atgagaggca acagaaataa ccccatccgc cccaaggttc atggcatgga
    12541 aagcatctcc atcctcacct gtataaatca agaactcttc aggcttgtgc tcaatcaagt
    12601 aagccatatt agccaagcta gtacattctt tgacaccgat aatatttgga tggtcagcca
    12661 agcgaagcat ggtttctgga gtcaattcga caactacacg ccctggaatg ttatagataa
    12721 taattggtag gtcagaagca tctgcaatag ccttaaagtg ctgatacatc ccttcttgag
    12781 aaggtttgtt gtagtaagga acaatagcaa gcccagctgc gaaaccacca aattccgcta
    12841 cttctttgac aaactcaata gagtcacgcg tatcattagt acctacaccc gcaatcaaag
    12901 gaacgcgtcc attgacaacc ttttgtacag ccgcaaacaa ctccaactcc tcatcgtggg
    12961 tcaaagttgg actctcagca gtcgttcctg cgagaagaat tccatccgta tgatgggcca
    13021 ataaatgctc aatcaaggct ggaatagcat caaagttaat ggaaccatcc tcatggaagg
    13081 gggtaataaa ggctgtaatg attttacatt cttttaaatc ttgataagac acgaaaacac
    13141 ctctctattt caaagaagga gttcatctga actcctaaac gatatgacta ttttaattca
    13201 aatttcaatt cggctgttgg acgaaccaat ccacgttcat gaagagtttc agcaatctga
    13261 actgagttcc aagcagcacc tttgagaagg ttatctgaaa caacccacat gtgaattcct
    13321 ttttctgcat ccaagtcttt acggatacga ccaacaaagg tatcacgcga accaactgca
    13381 ttgatagctt gaggatagat ttgatgagct acatcatctt caagaacagc acctgggaag
    13441 gctgcgatag ctgcttttac ttcttcgatt ggagccactt cttttgtttc gatataaaca
    13501 gactcagagt gagctgacaa gactggaata cgcacacatg ttgcagatac tgcaatgcta
    13561 tcatcttcca taattttctt agtttccttg gtcatcttca tctcttcgta cgtgtaatca
    13621 ttatcagtga aaacatcaat ttgtggaaga gcgttaaagg cgataggata atgtttcttg
    13681 tcaccacctg aaggcaaaat ttccgcatgc aaatcacatg gtttcacacc atcattcaag
    13741 acttcacgaa gttcacgttg tgtctcaaga attgctccca taccagcacc tgaaacggct
    13801 tgataagttg aaacaatgat acggtccaag ccccattttt ggcgaaccgg ctcaagagcc
    13861 accatcattt ggattgttga acaattaggg caggcaatga ttccgttgtg agcatcaagt
    13921 gcatgagcat tgacctctgg aacaaccaaa ggaacatctg gattttgacg gaaataagat
    13981 gtattatcta ctactaccac gccagctttt actgcgtatg gtgcatactt agctgatgta
    14041 gaactacctg ctgaaaagag agcaatatca actccttcaa aagctgtttc agtcgtttct
    14101 tcaattgtaa tatcttgatc tttaaatttc aatgacttac ctgctgaacg tgcagaagca
    14161 aggtaacgga ttttatcgat tggaagtgtt gattcttcca acatttttat catctgagca
    14221 ccgacggcac ctgtcgcgcc gactacagca actgtatatc ccataaataa cctctttcgg
    14281 aattttctaa aaatttctat aatagaagta ttatactact ttttccatga attgcaaagc
    14341 tttttcatca ttttttggaa aataaaaatc ccagtcgcta gactagggac taagaggcat
    14401 cttcatgtga tgagcaggtt cacacaactc atcaaggtcc gctcctgcgt tatgacctcc
    14461 ttatgctcaa tagtagtccg aagactacct atcgactcat cgcatattct atattactag
    14521 agatacttga ttttgtcaat aagaaatttt ttcgatcgag tataacaaaa aaactctcgt
    14581 attcaagagt ttccctatac atatcatgct aattgccggg attgaaccgg cgacctcatc
    14641 cttaccatgg atgcgctctg ccaactgagc taaatcagct tacttaagaa gtatactata
    14701 ttcagaaaaa cttgtcaagt ttccttagaa attttccata ggaaaaagcc aggtaagaac
    14761 tacctagctt atcttcttct atttacttaa atcaattcga cgaccaattt taccaataat
    14821 aatcgctcca ataatcaatg aagcaaattc accgattcct gttgtaaacc aagtaaggaa
    14881 gaatggtaat tctgctacaa tatttaactc tgcagcgata gtaaacatgg aaattgaaaa
    14941 gaggattgaa aagaagaaat gatcttttcg aatcaatccg ttaaagaggt aatccttgct
    15001 atattttgca aaaagccaaa cacctaaact gaggaatact aaggttgaac caccaccaac
    15061 aaagacatct agcagtccga agctaaagaa attagcaatc atacaaccga tggtaactcc
    15121 gatgatgtac ttaggattgt aaaaagccat aaagttcatc atttctgaaa tacgaaactg
    15181 ataagcacca tagctgatgg catttagggg cggtgtgaca gtcaaaacga cgtagatagc
    15241 agcgacgatt gcaatatctg caacatcacg aatagttaat tttttcatct tttctccttt
    15301 ggcggttgcc cgcgtgtaat atgcttggtg aaagaagcta agcaccaagg gttgctttat
    15361 tcaaccctac tagtatagca caatagcctg ctttatgcta tacttaacac atgaaaattc
    15421 ctattcaaaa attttttaac aatgaaatct tagcctatct cttttttggt atagtgacta
    15481 ctcttgttgc tatactatta cgattagtca tttatcagct aagtcatcaa gaactccttg
    15541 ctacggcctt ggcaaatatt atcggtattt tatttgcctt tatcacaaat gatacaattg
    15601 tatttaagca aaaaaagaaa aatagactta tccgtttagt gaaattttct cttgctcgcc
    15661 tttctacttt tctgttagac ttgtttttaa cttttctctt tgtaacccaa tttcctcaca
    15721 tcatagggca attcgtaact gaaaacattg ataagataaa tgctattgaa acaataattg
    15781 cacaaatatt cattattatc atcaattata tcttgagcaa ggtctatatt tttagaaaat
    15841 aatgctccat ttatgagcct tttaattgcc ttctataatg atttaggata caatgaaaca
    15901 cgaaacattg aaaggaaaac agtatgttaa aaaatctaaa atcgttcttg cttcgaggaa
    15961 atgttattga ccttgctgtc ggtgttgtaa ttgcctctgc ttttggtgct atcgttactt
    16021 cacttgtaaa cgacattatc actcctctta ttttaaatcc cgctttgaaa gctgctaaag
    16081 ttgaacgtat cgctcaactt tcttggcatg gagtcggcta tggtaacttc ttaagtgcta
    16141 ttatcaattt tatctttgtg ggtaccgccc tcttctttat tatcaagggc attgaaaaag
    16201 cacagaaact gactggcata aaggaagaaa aaactgccga aaaaaaacca accgaattgg
    16261 aagtccttca agaaataaaa gctctccttg agaaaaaata atcgattaaa aggatctagc
    16321 gcaaagctaa atcctttttt ctttactctt tgggtaactt gcctgctttt tcaagcattt
    16381 ttttgataag ataaggcatc ttaacattct cccgcccttt tttctcaatc agttttttca
    16441 caaattctgg catttgtaaa tcttgagatt cggctaagat atttttagtc tcatctgaag
    16501 gcactaactc atcaaaggtt tcttcttctg ggagaaattc cttaaactct tgattttcat
    16561 tgactctaac tgtattgacc aaggcatcaa tcaaacgaga atctgtattt ggcattggtg
    16621 gacgatggta gtttacccca aattcctgac ataaatcata acattccaca tcattgtcaa
    16681 acaagacctc aatatgctca ctgatgaaac taataggtac aaaaatataa tggtctggat
    16741 gttctgtctg ttctctgaga tactccaaaa catctggttt aatccatgga ataccaatat
    16801 cactctcact ctgccaagtg ttggtatatt gttctgaact caagcccagt ttttcagcga
    16861 ctagcttgct attttcgaaa atttggtcta tataaggatc tccaaagtcc aaggcaaaaa
    16921 taggcacact atgggctgaa aagatgactt taaagctgtc ttgctttact tcttctttta
    16981 aaatcttagc aatttcatct gcccaatagt ttaagagcgc ttcttcctga taccagtcct
    17041 taatgaccaa aaattgaatt tgtttgcttt ctaaaaattt ttcatagccc atgacagagt
    17101 agaaagaata atggggctcc aaaatcaagc aaatacactg ttcaattccg tctgcttcca
    17161 tttgaccgat cacatctgga ataaagggac tggaaaactt attagcaaag tagacactgt
    17221 attcattccc cagcctagct tctaccaagg caacctcttg acgggtaatt ttttgcagag
    17281 gcgtgcctcc aattcgtaca taattatcat agagagtctg aatctcgtgg tcttggggtc
    17341 tcatcccacg acgaatattt gtgaaaaaat cagctacacc ttcaaaggta atctcttctg
    17401 gtgaaccgaa ggtcatcatt aaaattgctt ttttcatgac tgcttccttg tgatgttttt
    17461 atcaagttta ttttatcatt aattcatcga taagtaaacg ctagcatagg gacattctca
    17521 atcttctgtc ttttgttttt ctcttctttc tatgatacaa tggaaaaaat aaattcaaaa
    17581 ggagtttttt tatgacttat ccaaatctct tggaccactt cttaacctac gttaaggtca
    17641 acacgcgctc tgatgaacac tctactacta ctccaagtac acagagtcag gttgacttcg
    17701 caacaaatgt cctaattcct gaaatgaaac gtgttggact gcaaaatgtt tactatctac
    17761 cgaatggttt tgctattgga accttgccag ccaacgatcc gtctttaaca cgtaagattg
    17821 gttttatatc gcacatggat actgctgatt ttaatgctga aggagtcaat ccacaggtaa
    17881 ttgaaaacta cgatggtggt gtgattgaac tagggaattc tggtttcaaa ctcgatccag
    17941 ctgacttcaa gagtcttgaa aaatatccag gacaaacgct catcacaaca gatggaacaa
    18001 ccttgctagg tgctgatgac aagtcaggaa ttgctgaaat tatgacagcc attgaatatc
    18061 taactgctca tcctgaaatt aagcactgtg agattcgtgt tggttttggt ccagatgaag
    18121 aaatcggtgt tggtgccaat aaatttgatg cagaagattt tgatgtggat tttgcctaca
    18181 ctgttgacgg tggtccacta ggtgaacttc agtacgagac tttctcagcc gctggtgctg
    18241 aattgcattt ccaaggccgt aatgtccacc ctggtactgc caaagggcag atggtcaatg
    18301 cccttcagct agcaattgat tttcataatc aacttccaga aaatgaccga cctgagttaa
    18361 ctgaaggtta ccaaggtttt taccatctaa tggatgtgac aggtagtgtt gaggaggtgc
    18421 gtgcaagcta catcattcgt gattttgaaa aagatgcctt tgaagcgcgt aaagcatcca
    18481 tgcaatctat cgctgataag atgaatgaag aacttgggag cgaccgtgtc actctcaact
    18541 tgacagacca gtactacaat atgaaagaag tcattgaaaa agatatgact ccaattacca
    18601 ttgctaaagc cgttatggaa gatctaggta tcacgcctat tatcgaacca atccggggtg
    18661 gaacagacgg ctctaagatt tcctttatgg gaatcccaac tccgaatatc tttgcaggtg
    18721 gcgaaaatat gcacggacgt tttgaatacg ttagccttca gactatggaa cgtgcagttg
    18781 ataccatcat tggcatcgta gcttataaag gctaaaaaga cgaggtagct cagctacttc
    18841 gcctttcttt ttattctact ggtttttctt gatttccagt agttgtagaa gattctgttg
    18901 tttcattttc tgaagttgat tcagcaggtt tagaatctct tgtattgctt ggtttgtttt
    18961 cgtcgctagc agtttcaatg ttagattctg cagttgcgtt tggttggttc tcagcactgg
    19021 tgttatcact atttgcttca gcatttcttg ctggacttgt ttcttcactt gcgctagctt
    19081 ttgactggat ttgatgattc aaaactagaa tagcttttgt cgattcaagt aaagctgttt
    19141 tgtctttact cttagcagaa agttgatcta ataatgcatc caccttatca aagtccgcat
    19201 cagatccatt attactttct aaataagagt gaagcgacat gagaatatcg tagagttttt
    19261 gatagagtac aagtgtctga gaatcttgct cagcattttc cttttcttgt tgaagggcgc
    19321 tagcgatacg agtcaaaaca tcttttacct gactgtttac ttcatccaag tctgcatcag
    19381 ccttgtttgt ggcagctttt agattttcta cttcttctgc caaggattgt ctgattcctt
    19441 cttcatggat ttgttccaag agttgatttg ccttgttcaa aaaactttct acttcttcct
    19501 tgctatctgt cgcagattat tggttgctat ctaccatgta ctcctaaaac aggagagtta
    19561 taatccaaga ttacaaggcc ttacagaaat aagaaatcca gataagacaa tgttcgtcca
    19621 agacgctatt cgcttcgcac agcagcacgg attcaatata ctttaatttt aaagtttagg
    19681 tgtcaagacc tctttttagt gtgcccaaaa tttagagaag taatcaatca actaactttt
    19741 atttttttca aactttcagt aaactgacct aaagctaact caatctgtct ttgttcgata
    19801 ggcttgtctt tgtagatgct tctgctatca gctagaagtt gatctacttt tgccaagact
    19861 gccttctcat caaaagttcc aggttgatag ttggattgca gggatggaat cttgtttttc
    19921 aaagccgctt catatccctt agtttgaacc ttgatgtagt gattgtggtc gccacgagga
    19981 atcacaaaac cttctgaatc ttcacttata attcgattgg catcaaaacc atgaccatct
    20041 tcttcctcat ggtggacatg tagtgacgga ttacttaata cagaactaga agaacttcct
    20101 accttttccg tgttagagtg tgatggggga ttgttaagag atgacttagg aatatagtga
    20161 tagtgacccc atgtcttact atataagcat cacctgtatc tctgacaata tcattagggt
    20221 taaagacata accatcatct gctgcagaaa caccattatt cggtgtcacc gacaaagatt
    20281 gactgagagc tgtagtattc tctgataatt atacttttgc agctgctaat tcacctgccg
    20341 acaagtcact ctcaggaatg aaatgatagt gaccaccatg tggtactata gtagattgaa
    20401 atagaatatg agcaaattga taaggggatt ttaaagtaat ttctaacaat gatttagaaa
    20461 ctatgatgtg ctattctaaa ttcaactcac tatatataac catcatcggt agtataacgt
    20521 ccctgtaatt ttgctacaga tacttctgca ctagctcctt tatcgtcttt accatgttct
    20581 tgtttttggc gattgatttc atcttttatt cgtacatttt ctgcatgagc ttgatcttta
    20641 aggtaaacat aatactttcc atctacctta ataatatatc ctcccttaac ctaactgacg
    20701 atatcttgat ctttcggctg atagttgggg gctttcatta atagctcttc actaaagagc
    20761 gcatacaaag gaactttacc attatagcag tgataatgat cgccatgaga agttacataa
    20821 ccttgatctg taatcttaat aacaatttgt tttgcttgaa ttccttcttt ttgactaacc
    20881 tagtctggag tcaaattttc agtcttctta gtgtctttat tactgtttac atatgaaaca
    20941 cgatttttat ctgtattggc ctgttagcta tgttggttca gagcataaac acacagactt
    21001 aaggaaagga taacaacaga tccagctgtt atatatttct ttttaaattt cataattacc
    21061 tcatttctat
//
LOCUS       DBERDK010000031        20975 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.031, whole genome shotgun sequence.
ACCESSION   DBERDK010000031 DBERDK010000000
VERSION     DBERDK010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 20975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20975
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.031"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..614)
                     /locus_tag="ACL73L_001154"
     CDS             complement(<1..614)
                     /locus_tag="ACL73L_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000530076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HGS0772140.1"
                     /translation="MEQLHFITKLLDIKDTNIQIIDVVNRDSHKEIIAKLDYDAPSCP
                     ECGNQLKKYDFQKPSKIPYLETTGMPSRILLRKRRFKCYHCSKMMVAETPLVKKNHQI
                     PRIINQKIAQKLIEKISMTDIAHQLAISTSTVIRKLNDFHFEHDFSRLPKIMSWDEYA
                     FTKGKMSFIAQDFDNLNIITVLEGRTQAVIRNHFLRYDRAVRCQV"
     gene            796..2175
                     /gene="bglA"
                     /locus_tag="ACL73L_001155"
     CDS             796..2175
                     /gene="bglA"
                     /locus_tag="ACL73L_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HGS0772141.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSDKTSTFYENWERDIELLLETGHTAFRTSIQWSRIFPQGCGKVNPQGVDFYRK
                     VFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
                     WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDA
                     TEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPESPLLPEHFYEPYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWF
                     KELSDNNGF"
     gene            2367..4175
                     /gene="glmS"
                     /locus_tag="ACL73L_001156"
     CDS             2367..4175
                     /gene="glmS"
                     /locus_tag="ACL73L_001156"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HGS0772142.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVLDGADNHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSETERFVLVHNGVIE
                     NYLEIKEKYLAGHHFKGQTDTEIAVHLIGKFAEEDGLSVLEAFKRALHIIRGSYAFAL
                     IDSENPDVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDQELVIVKADS
                     VEVQDYDGNRRERASYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDEAGQ
                     VVVDPDIIKAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYGMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREANYTMLLHAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQAFDLVHELSIVAQSIESTLSEKET
                     IEAKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLALLSDPVLANHTRGNIQEVAARGAKVLTIAEENVAKDTDDIVLTTVHPYLS
                     PISMVVPTQLVAYFATLHRGLDVDKPRNLAKSVTVE"
     gene            complement(4252..4389)
                     /locus_tag="ACL73L_001157"
     CDS             complement(4252..4389)
                     /locus_tag="ACL73L_001157"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772143.1"
                     /translation="MKIKEQTRKLAAGCSKQCFEVVDRTDEISNHTYGKATLTWFEEI
                     F"
     gene            4446..5495
                     /locus_tag="ACL73L_001158"
     CDS             4446..5495
                     /locus_tag="ACL73L_001158"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HGS0772144.1"
                     /translation="MVELGISTFGEITELEGTGQTYSHAERIRQLVAEIELADKVGLD
                     VYGIGEHHRADFAVSAPEIVLTAGAVNTKKIRLTSAVSILSSMDPIRLFQQYVTIDAL
                     SNGRAEIMAGRGSFTESFPLFGYDLKDYDSLFDEKLDLLQLVNEKTKLDWQGRLTQTI
                     AGKEVYPRPVQDKLPLWIATGGHVESTVKIAQAGLPIVYAIIGGNPRYFKKLIQAYRE
                     IGSEAGHADKDLKVGAHSWGWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQ
                     YLEQVGPNGAMFVGNPDQVAEKLIRMIEDLGLDRFMLHLPLGSMPHDQVLRAIELFGT
                     QVAPKVRAYFAMKEA"
     gene            complement(5595..9392)
                     /gene="spuA"
                     /locus_tag="ACL73L_001159"
     CDS             complement(5595..9392)
                     /gene="spuA"
                     /locus_tag="ACL73L_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HGS0772145.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISS
                     DESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNESDISSPRNANASLEKTEEKPATEPT
                     TSTSPVTTETKSEEPIEDNYFRIHVKKLPEENKDAQGLWTWDDVEKPSENWPNGALSF
                     KDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNLTGDKSVEKLVPKMNEAWLDQDY
                     KVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVKNPSSAQWPDGTDFTATGKYGRY
                     IDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFTDLKNHSQIFLKDDDESIYTNPY
                     YVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKHSNITNHLGNKVTITDVAIDEAG
                     KKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWRLKDETYSYDGKLGADLKEEGKQVDLT
                     LWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTWKQTLDSTNKLGITDFTGYYYQY
                     QIERQGKTVLALDPYAKSLAAWNSDDAKIDDAHKVAKAAFVDPAKLGPQDLTYGKIHN
                     FKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEAFIEKLDYLKDLGVTHIQLLPVL
                     SYYFVNELKNHERLSDYASSNSNYNWGYDPQNYFSLTGMYSSDPKNPEKRIAEFKNLI
                     NEIHKRGMGAILDVVYNHTAKVDIFEDLEPNYYHFMDADGTPRTSFGGGRLGTTHHMT
                     KRLLVDSIKYLVDTYKVDGFRFDMMGDHDAASIEEAYKAARALNPNLIMLGEGWRTYA
                     GDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGYPNEGQPAFITGGKRDVNTIFKN
                     LIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSIKKDPSKAENYAEIHRRLRLGNL
                     MVLTAQGTPFIHSGQEYGRTKQFRDPAYKTPVAEDKVPNKSHLLRDKDGNPFDYPYFI
                     HDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKGLIALRQSTDAFRLKSLQDIKDR
                     VHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVNADEKAREFNLGTAFAHLRNAEV
                     LADENQAGSVGIANPKGLEWTEKGLKLNALTATVLRVSQNGTSHESTAEEKPDSTPSK
                     PEHQNEASHPAHQDPAPEARPDSTKPDAKVADAENKPSQATADSQAEQPAQEAQASSV
                     KEAVRNESVENSSKENIPATPDKQAELPNTGIKNENKLLFAGISLLALLGLGFLLKNK
                     KEN"
     gene            9808..10221
                     /gene="rpsL"
                     /locus_tag="ACL73L_001160"
     CDS             9808..10221
                     /gene="rpsL"
                     /locus_tag="ACL73L_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HGS0772146.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            10241..10711
                     /gene="rpsG"
                     /locus_tag="ACL73L_001161"
     CDS             10241..10711
                     /gene="rpsG"
                     /locus_tag="ACL73L_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HGS0772147.1"
                     /translation="MSRKNRAPKRDVLPDPLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            11136..13217
                     /gene="fusA"
                     /locus_tag="ACL73L_001162"
     CDS             11136..13217
                     /gene="fusA"
                     /locus_tag="ACL73L_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HGS0772148.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLIEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEETRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEK
                     AKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
     gene            13321..17712
                     /locus_tag="ACL73L_001163"
     CDS             13321..17712
                     /locus_tag="ACL73L_001163"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HGS0772149.1"
                     /translation="MSNSFEILMNQLGMPAEMRQAPALAQANIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFEIKARSQEFSNQLLQSYYREAFSEGP
                     CASQGFKSLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFPTFNCQ
                     VEKNDVLTQEQEEAFHAENEQIVQAANEEALRAMEQLEQMTPPPAEEKPVFDFQAKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLTMNVQDLQEVVHYERKDLMPE
                     GERRVEFHAHTNMSTMDALPEVEEIVATAAKWGHKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGMEANIVEDRVPIVYNEVEMDLSEATYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGHPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNATFDVGFMNANYERHDLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLARLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVSRIPRTVLDAHREGLILGSACSEGEVFDVVVSQGVD
                     AAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKSLIEVGDRLGKPVLATGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFEPVEVVKGDLYTPFIDKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPHKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVERLAQGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQ
                     TTHFNFHDIDENVLKLDVLGHDDPTMIRKLQDLSGIDPNEIPMDDEGVMALFSGTDVL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQD
                     LIKQGIADLSTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNDYIE
                     AMKANKVPEWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAK
                     AFDIKTMGAGLEVIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLD
                     LYCSQATEFLIDGDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSST
                     LVEKMDEMGILGNMPEDNQLSLFDELF"
     gene            17814..18077
                     /locus_tag="ACL73L_001164"
     CDS             17814..18077
                     /locus_tag="ACL73L_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HGS0772150.1"
                     /translation="MSKMSISIRLDSEVKEQAQQVFSNLGMDMTTAINIFLRQAIQYQ
                     GLPFDVRLDENRKLLQVLTDLDQNRNMSQSFESVSDLMEDLRA"
     gene            18070..18348
                     /locus_tag="ACL73L_001165"
     CDS             18070..18348
                     /locus_tag="ACL73L_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HGS0772151.1"
                     /translation="MLKIRYHKQFKKDFKLAMKRGLKAELLEEVLNFLVQEKEHPARY
                     RDHSLTASKHFQGVRECHTQPDWLLVYKVDKSELILNLLRTGSHSDLF"
     gene            18369..18521
                     /locus_tag="ACL73L_001166"
     CDS             18369..18521
                     /locus_tag="ACL73L_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772152.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLSGAIALAGIF
                     SALREK"
     gene            18543..19784
                     /locus_tag="ACL73L_001167"
     CDS             18543..19784
                     /locus_tag="ACL73L_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HGS0772153.1"
                     /translation="MVLPNFKENLEKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYALGAHEVIVQWTDDVINREKFLHAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGALNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NAASDEEAVDFLWDQIFKTCRVYEADPVKAWEEHAAILKSKADMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAVNAQGEGFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGQIVDITAEKGDQVMKDLVFENAGARALGECALVPDPSPISQSGI
                     TFFNTLFDENASNHLAIGAAYATSVVGGAEMSEEELEAAGLNRSDVHVDFMIGSNQMD
                     IDGIREDGTRVPLFRNGNWAN"
     gene            19794..20024
                     /locus_tag="ACL73L_001168"
     CDS             19794..20024
                     /locus_tag="ACL73L_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HGS0772154.1"
                     /translation="MLGSMFVGLLVGFLAGAMTNRGERMGCFGKMFLGWIGAFLGHLL
                     FGTWGPVLSGTAIIPAVLGAMIVLAIFWRRGS"
     gene            20247..20969
                     /locus_tag="ACL73L_001169"
     CDS             20247..20969
                     /locus_tag="ACL73L_001169"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HGS0772155.1"
                     /translation="MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKLQIDEK
                     IDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKHRTVLDLLDDLARSKEVFPVGRL
                     DIDTHGLLLLTNDGQLAHVLLSPKRHVDKTYLAQVKGIMTQEDVETFAEGIPLKDFTC
                     QPAILELVSIDTEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDEN
                     LERGEWRRLTKEELEILRANII"
BASE COUNT         5896 a         4027 c         4953 g         6099 t
ORIGIN      
        1 acttgacaac gaacggctct atcgtagcga agaaagtgat ttcggatgac agcttgtgtt
       61 ctgccttcaa gaacagtgat aatattaaga ttatcaaaat cttgcgcaat gaaactcatc
      121 tttcccttag tgaaggcata ctcatcccaa gacataatct ttggaagccg agaaaaatca
      181 tgctcaaagt gaaagtcatt gagcttgcga atgacagttg aagttgaaat ggccagctga
      241 tgggcaatat cagtcataga aattttttca attaactttt gagcaatttt ttggttgatg
      301 atacgaggga tttggtgatt tttctttacc aggggagtct cagcaaccat catttttgaa
      361 cagtgatagc acttgaaacg gcgttttcta aggagaattc tagaaggcat accagtcgtt
      421 tcaagataag gaattttaga aggtttttga aagtcatatt tcttcaattg gtttccgcac
      481 tcagggcaag atggggcgtc gtagtccagt ttggcgatga tttccttgtg tgaatcccta
      541 ttgacgacat ctataatttg gatatttgtg tctttgatat cgagtagttt tgtgataaaa
      601 tgtaattgtt ccatatgaat ctttctaatg agttgtttgg tcgcttttca ttatagatct
      661 tatgggactt ttttctacaa taaaataggc tccataatat ctatagtgga tttacccact
      721 acaaatatta tagagccgtt tttaccttgt tttttctgta taatgggaga aatgagtaga
      781 taaagaggta aatgtatgct aagatttcca aaggattttg tctggggatc ctctacttct
      841 ggaccgcaaa cagaaggacg tgtagctggt gacggtaagg gagacaatct ctgggattac
      901 tggttccaag tggagccaaa tcgttactat aatgggattg gttcagataa gacatcgact
      961 ttttatgaaa attgggagcg ggatattgag cttttgttag agactggtca cacagccttt
     1021 cggacttcta ttcagtggtc acggattttt ccacaaggct gtggaaaagt caaccctcaa
     1081 ggtgtggatt tttatcgtaa ggtctttgag gctattaagg ctaaaggaat tcgtctgtta
     1141 gtcaatctct atcattttga tttacctttt gcccttcaag aggatggtga tggttgggaa
     1201 aataaggcga cagtctcagc ctatgaagac tatgctcgtt tttgttttga gacttatgga
     1261 gatttagtgg atcaatggat tacctttaac gagcccatcg ttcctgtaga atttggttat
     1321 ttttatgatg cccattatcc acataaggtg gatgcagagg cagctgttaa ggtagcctat
     1381 catacacaat tggccagcag ccgggctgtt aaggcttgcc atgaactttt gcctgattcc
     1441 aagattggga ttgtcctcaa cttgacaccg gcttatccac gtagccagca tcctgctgat
     1501 gtcaaggcag ctcgtattgc ggcccttttt caggcccaat ctttcttaga tccatctgtc
     1561 ttgggaactt atccacagga gttagtagaa atcttgcatg aacacggtct tttacctgat
     1621 gctacagagg aagagttgga actcattcgt gataatacgg tggacttcct tggtgtgaac
     1681 tactatcaac ctttgcgtgt tatggctcct cgatttgcta agcatccaga gagtccactc
     1741 ttaccagaac atttttacga gccttatgtg atgcctggac gtaaaatcaa ttctcaccgt
     1801 ggttgggaga tttatgagca agggatttat gacatcgccc aaaatatcaa ggaaaattat
     1861 ggcaatattg agtggatgtt gactgagaat ggtatgggtg ttgaagggga agaaaaattc
     1921 cgtcaagatg gaatgattca agatgattac cgtattgact ttgtaaaagg tcatcttcgt
     1981 gaacttcacc gtgccattga agatggtgcc aactgtaagg gctacttgat ttggaccttt
     2041 attgactgct ggtcatggct caacagctat aaaaatcgct atggtttggt cgaattagac
     2101 ttggaaacgc aagaacgtcg tctgaagaaa tcaggccact ggttcaaaga attaagcgat
     2161 aataatggat tttaaaaagg actctgactg attatcccaa tcggtcagag ttttttgctt
     2221 acaggagctt aatttgaact ataccaattt ttactcttga caagtgaaag aaacaagtat
     2281 atactgtttt tgctgattct ataaaagatg aattagacta taccaatttt aaggagaaag
     2341 cacagcttgc ctgtgtcgta tatactatgt gtggaattgt tggtgttgtt ggaaacacaa
     2401 atgcaactga tattttgatt caagggcttg aaaagcttga ataccgtggc tatgattctg
     2461 cgggaatttt tgtcctagat ggtgctgata accatttggt gaaggctgtt ggtcgtatcg
     2521 cagaattgtc tgccaagaca gctggtgttg agggaacaac tggtatcgga catactcgtt
     2581 gggcaactca tgggaaacca acggaagaca atgctcaccc acaccgatct gagacagaac
     2641 gttttgtctt ggttcacaat ggggtaattg aaaactacct tgaaattaaa gaaaaatacc
     2701 ttgctgggca ccacttcaaa gggcaaacag atacggaaat cgccgtacat ttgattggaa
     2761 aatttgcgga agaagacggt ctctcagttc ttgaagcctt taaaagagct cttcatatta
     2821 tccgtggttc atatgccttt gccttgattg actctgaaaa tccagatgtc atctatgtag
     2881 cgaaaaacaa atctccactt ttgattggtc ttggggaagg ctacaatatg gtctgctcag
     2941 acgccatggc tatgattcgt gaaactaacc aatacatgga aatccatgac caagagttgg
     3001 taatcgtcaa ggctgatagt gtggaagttc aagactatga tggaaatcgt cgtgagcgtg
     3061 ctagctacac tgctgaactt gacttgtcag atatcggtaa gggaacttac ccttactaca
     3121 tgctcaagga aattgacgag caaccaactg ttatgcggaa actcatccaa gcctacacag
     3181 atgaggctgg tcaagttgtc gtagacccag atatcattaa ggctgttcaa gacgcagacc
     3241 gcatctacat ccttgcagct ggaacatctt accatgcagg atttgcttct aagaaaatgt
     3301 tggaagaatt gacagataca ccagttgaac ttggaatctc atctgagtgg ggctacggta
     3361 tgccacttct cagcaagaaa ccactcttca tctttatcag ccaatctggt gaaacagcgg
     3421 atagtcgtca agttttggtc aaggctaatg aaatgggaat tccaagctta acagtgacaa
     3481 acgttccagg ttcaaccctc tcacgtgaag ccaactatac catgctcctt cacgcaggtc
     3541 ctgaaattgc cgtggcatca actaaagcct atacagcgca aatcgcagcc cttgccttcc
     3601 ttgcaaaagc agtcggagaa gcaaatggta atgctaaagc gcaagccttt gacctggttc
     3661 atgaattgtc aatcgtagct cagtctatcg aatcaactct ttcagagaaa gaaaccattg
     3721 aagccaaggt tcgtgaactt cttgaaacaa ctcgtaacgc cttttacatc ggacgtggtc
     3781 aagattacta cgtagccatg gaagcaagtc tcaaactcaa agagatttct tatatccagt
     3841 gtgaaggttt tgcggcagga gaactcaagc acggaaccat tgccttgatt gaagaaggaa
     3901 cgcctgtttt ggctctcttg tcagatccag ttcttgccaa ccatactcgt ggaaatatcc
     3961 aagaggtcgc agcccgtggt gccaaagtcc tcactatcgc agaagagaat gtagccaaag
     4021 ataccgacga tatcgtcctt acgaccgtac atccatacct ctcaccaatt tcaatggtcg
     4081 taccaacgca attagtcgct tactttgcaa ccctccaccg tggcctcgat gtggataaac
     4141 cacgtaacct tgctaaatca gtaacggtag aataagctaa aaaagtctag ttatctaggc
     4201 tttttctaat gagcaaattg gttgcttgta cttaagattc gtgttatact cttagaaaat
     4261 ctcttcaaac cacgtcaacg tcgccttgcc gtaggtatgg ttactgattt cgtcagttct
     4321 atccacaacc tcaaaacact gttttgagca acctgcggct agcttcctag tttgctcttt
     4381 gattttcatt gagtattaga ataatttttc aaattgaagg acagagatag acaaggagaa
     4441 tcacaatggt agaattggga atttcaacat ttggggaaat aacggagctt gaagggactg
     4501 gacaaactta cagtcatgct gaacgcattc gccagttggt ggcagagatt gagctggctg
     4561 acaaggttgg tttggacgta tatgggattg gcgagcacca tcgggcggat tttgcagtat
     4621 cagccccaga gattgtcctg accgctgggg cagtcaatac caagaaaatc cgtttgacca
     4681 gtgcagtcag cattctgtca agcatggacc cgattcgttt gttccaacag tatgtcacta
     4741 tcgatgcttt gtcaaatgga cgagcggaga ttatggctgg aaggggctct ttcacggaat
     4801 ctttccctct gtttggttat gacttgaaag actacgactc tctttttgat gagaaattag
     4861 acttgcttca gttagtcaat gaaaagacca agttagactg gcaaggtcga ttgactcaaa
     4921 cgattgctgg taaagaagtt tatcctcgtc cagttcagga caaattgccc ttgtggatag
     4981 ctacaggtgg tcatgtcgaa tcaacagtga agattgctca ggctggtcta ccgattgtat
     5041 atgctattat tggtggtaat ccacgttatt ttaaaaagtt gattcaggct tatcgtgaga
     5101 ttggaagcga agcgggccat gctgacaaag acttgaaagt gggagcccat tcttgggggt
     5161 ggattgcgga agatggtgag caggcggtga aagattattt ccatccgact aagcaagtgg
     5221 tggatgctat ttccaaagac cgtccgcact ggcaggaatt gcgttatgag caatatttgg
     5281 agcaggtagg gccaaatggc gccatgtttg ttggcaatcc agatcaggtg gccgaaaaat
     5341 tgattcgcat gatagaggat ttaggattgg accgcttcat gcttcatcta ccgcttggtt
     5401 ctatgcctca tgaccaggtt ctgagagcta ttgaactctt cggaacacaa gttgcaccca
     5461 aagtacgagc ttattttgct atgaaagagg cttaataaaa aatcctaatc aagtttatta
     5521 ggacaacaaa tattgtacaa aaattaatcc cccgagctat aatgcttggg ggatttttct
     5581 ataggagggc tagtttagtt ctctttttta ttttttagta agaaaccgag accaaggagc
     5641 gcaaggaggc tgattcctgc aaataggagt ttgttttcgt ttttgattcc tgtatttgga
     5701 agttcagctt gtttatctgg ggttgcaggt atattttcct tgctagagtt ttctaccgat
     5761 tcgtttcgaa ccgcttcttt tacagatgat gcttgtgctt cttgtgctgg ttgttcagct
     5821 tgtgaatcag ctgtagcttg gctaggttta ttttccgcat cagctacttt ggcatctggt
     5881 ttagtagaat caggtctagc ttctggagct gggtcttgat gtgcagggtg agaagcttca
     5941 ttttgatgtt caggcttgga aggggttgag tctggtttct cttctgcagt tgactcatgg
     6001 ctagttccat tttgagagac tcgaagaaca gtagctgtaa gggcattcaa tttcaagcct
     6061 ttttcagtcc attcaagtcc tttcgggttg gcaattccga ctgatcctgc ttggttttca
     6121 tctgccaaaa cttccgcatt tcttagatgt gcaaaggcag ttcccaaatt aaattcgcga
     6181 gctttttcat ccgcattgac aaagactgcg tagatatcgc cgtttggagc agtgatttgg
     6241 tagccaatca ctacatcctc tttttccaca ccattttggc ctgggacagt gatgaggtgg
     6301 acacggtctt tgatatcttg gagactctta agtcggaagg catctgtaga ttgacgaagg
     6361 gcaatcaaac ctttcatata gtcacggctc ttgacatttt caggataagc tttaccatct
     6421 gtagccttag tccagtcaaa cttgttgact gcgtcgctag aatcgtaaga atcatggatg
     6481 aagtaaggat agtcaaatgg gttgccgtcc ttatcacgca acaagtgaga tttgtttgga
     6541 accttatcct ctgctactgg agtcttgtag gctgggtcac ggaattgttt agtacgtcca
     6601 tattcctgac cggagtggat aaatggagtt ccttgagctg tcaagaccat gagatttcca
     6661 agtcgtaaac gacggtggat ttcagcatag ttctcagcct tgcttgggtc ttttttgata
     6721 gactgggcaa tgatgtcaaa gagggtcaag ttatcatggg ctgcgatgta ttggatgaca
     6781 tctccagggc tgtcagcttc aaagttagtt ggttgagcaa tgagattttt aaagatggtg
     6841 ttgacatcac gcttgccacc tgtgataaag gcaggttgac cttcgtttgg ataaccagat
     6901 ttgaggttgt tacggatgtc atctgaaaag acagcgacag tatcggtatg tttcatccaa
     6961 tcttggtcag cagctttagt aggcatgttt tcatcaccgg cataggttct ccaaccttca
     7021 ccaagcatga tgaggtttgg attgagggcg cgtgcagcct tgtaagcttc ttcgatagaa
     7081 gcggcgtcat ggtctcccat catatcgaag cggaagccat ccactttgta ggtatcaact
     7141 aggtatttga tagagtcaac taggagccgt ttggtcatat ggtgggttgt ccccaagcgt
     7201 ccaccaccaa agctagttcg aggtgtgcca tcggcatcca taaagtggta gtagtttggc
     7261 tctaggtctt caaagatatc gactttggct gtgtggttat aaacgacatc taggatagct
     7321 cccataccac gtttgtggat ttcgttgatg aggtttttaa attctgcgat tcgtttttct
     7381 ggattcttag gatcgcttga gtacatacca gtcaaggaga agtagttttg agggtcatat
     7441 ccccagttgt agttgctgtt gcttgaagcg tagtcagaca agcgttcatg gtttttcaat
     7501 tcattgacaa agtagtaaga caagactgga aggagctgga tatgggttac acccaagtct
     7561 ttgagatagt ctagtttttc aatgaaggct tcaaaagtcc caaatggttt ggtcaagtct
     7621 tttgcaatgg caggatctga agtgaagtca cgcacatgag cttcgtagat aacggcgtct
     7681 tcacgagtct tgaaattgtg aatcttacca taagtcaagt cttgaggtcc gagtttagct
     7741 ggatctacaa aggcggcttt agccacttta tgggcatcgt caattttggc atcgtcgcta
     7801 ttccaagcag caagagattt agcgtaagga tcgagtgcaa gaacagtttt accttgacgc
     7861 tcgatttggt attgataata gtagccagtg aaatctgtga ttccgagttt gtttgtgctg
     7921 tctagagttt gtttccaagt tcctctttcc cctttttcga gagcgacagt tccaactact
     7981 ttgtcagggt cattcttgtc gtagacaaca acagaaacct tatcagcact tggtgaccaa
     8041 agggtcaaat caacttgttt tccttcttct tttaggtcag ctcccagttt gccatcatag
     8101 ctgtatgtct catctttcag gcgccagctt gttttggtag tgaattggtc ggaattgtag
     8161 ctaacagtat aaggatgttt tgtgtcagag aaatctccgc tgtaggtcac tttcttacca
     8221 gcttcatcga ttgcaacatc ggtaatagtt accttgtttc ctaggtgatt agtgatgttg
     8281 gagtgtttga ggatatcttc ttttttagca ccgacaagtg ttgaaaagct actttcaatg
     8341 ctagaagtgc ctacgtgttg ggctcctgtc atacggatat catggacata gtatggattt
     8401 gtgtaaatcg attcatcatc gtcttttagg aaaatttggc tatgattttt caaatctgtg
     8461 aacttataat tttctttacg gattttcacg tcgtctcctt gtttgctctc atctagtaat
     8521 aaaaatccaa attctcttgc ggcttcatta agaggaatgt cgatatagcg gccatatttg
     8581 cctgtagccg taaagtctgt tccgtcaggc cattgagcgc tacttggatt ttttacatct
     8641 ccccagtacc agagagattt cttgtcatag ttgccatctg tgcggtagta gttgacgcga
     8701 acagttcctg caggctgtgg ctcgtaagag aaaaccttgt aatcttggtc taaccaagcc
     8761 tcgttcattt ttggaactag tttttctaca gatttatcgc cggttagatt ttttccagct
     8821 gtattgttga tgaggaagct aattttcttg gcttgttctc cctttaattt gacatctagg
     8881 taatagccgt agtcatcttt cttggcatcc ttgaaggaca aagctccgtt tggccagttt
     8941 tcagatggtt tttcaacatc gtcccaagtc catagtcctt gagcatcctt gttttcttca
     9001 ggaagttttt tgacatggat acggaagtag ttatcttcga tgggctcttc tgacttagtt
     9061 tcagttgtta ctggactagt agaagtagtt ggctctgttg caggtttttc ttctgttttc
     9121 tctagggaag cgtttgcatt tctaggtgaa gaaatatcac tttcgttctt accagagcca
     9181 gctgctgtat cagtaagagg ttgggcaagg gtggttgtat ttctgttgtt gggttcgtta
     9241 gtggttggtg tactttcgtc ggatgagata gttggtgtag ccatggcaag caagacaagg
     9301 cttgcgccga taaggacaga accagttcca tttttgaggg aacggatgct gtagaccatt
     9361 tttttctcag tgtgagatgg tgtttttctc ataatgattc tccaatcaat aaatttctat
     9421 atcagtatag catgtagtaa aacaatatgc aagcgttttc tttgatttgt gaaaaaattc
     9481 ttacttttga gaagttttct gtactatttt atatatcagt ataaatgacg gagataaaaa
     9541 tgtcacataa gccaagccgt attattagga agatgatgtg ttcccagcta tttttattca
     9601 gaaattgatc tctaacatta ccaataaaaa agaaaaatat cttgacaagc aagggaaaat
     9661 tttgttacaa taatagacgg tactttttac ttttggtctc tcaagagtgt acagggacgt
     9721 gctgacaaat gttgcaaaag tacacacaga tgatatctgt caccaagtgt atcatcacca
     9781 aaaataaaaa aacacaggag aatgtagatg cctacaatta accaattggt tcgcaaaccg
     9841 cgtaaatcaa aagtagaaaa atctaaatca ccagctttga acgttggtta caatagtcat
     9901 aaaaaagttc aaacaaacgt ttcttcacca caaaaacgtg gtgttgcaac tcgtgttgga
     9961 acaatgacac ctaaaaaacc taactcagcc cttcgtaaat tcgctcgtgt acgtttgagc
    10021 aaccttatcg aagttactgc ctacatccca ggtatcggac acaacttgca agagcacagc
    10081 gtggtgcttc ttcgcggtgg acgtgtaaaa gaccttccag gggtacgtta ccatatcgtc
    10141 cgtggtgcac ttgatactgc aggtgttaac gatcgtaaac aaggtcgttc taaatacggt
    10201 actaaacgtc caaaagcata aggaaagggg ataaagagaa atgagtcgta aaaatagagc
    10261 tccaaaacgt gacgtattgc cagatccgct atacaattca caactagtta ctcgtcttat
    10321 caaccgcgtt atgcttgatg gtaaacgtgg tactgctgct tcaatcgttt acggtgcttt
    10381 tgagcaaatc aaagaagcta ctggcaacga tgcacttgaa gtatttgaaa cagctatgga
    10441 aaacatcatg cctgtacttg aagtacgtgc acgtcgtgtt ggtggttcta actaccaagt
    10501 cccagttgaa gttcgtccag aacgtcgtac aacacttgga cttcgttggt tggtaacaat
    10561 cgctcgtctt cgtggtgaac acacaatgca agaccgtctt gcaaaagaaa tcttggatgc
    10621 tgctaacaac actggtgcag cagttaagaa acgtgaagat actcaccgta tggctgaagc
    10681 taaccgtgca ttcgcacact tccgttggta agataggatg cgaaagcgtt aagaaagtcc
    10741 cagagaaaat agggaatcga agcaggttgc ggttgcaacc aatgagattc atctttttct
    10801 ccagactttt agcttgagct caactaaatc atgatgctag gaacggtaag gatgcaaggt
    10861 gaaaatagga aactgacgca gtattcgacg aatacaagga gttttatctt tttcacgcag
    10921 catcccgttc cagctcacat cggctaacta actttagccc gggttcaaat tagctaaatc
    10981 gattagtatt agctataact cagcttacca tctcgtaagt tgaaaccaac aatagcatga
    11041 aaacattgag aacgggtagg tcctgcctat ccgtttttat taaaatcgtg ttataataga
    11101 atagaaatca aaaataaata ggagaaacaa acctcatggc acgcgaattt tcacttgaaa
    11161 aaactcgtaa tatcggtatc atggctcacg tcgatgccgg taaaacaaca actactgagc
    11221 gtattcttta ctacactggt aaaatccaca aaatcggtga aactcacgaa ggtgcgtcac
    11281 aaatggactg gatggagcaa gagcaagaac gtggtatcac gatcacatct gctgcgacaa
    11341 cagctcaatg gaacaaccac cgcgtaaaca tcatcgacac accaggacac gtggacttca
    11401 caatcgaagt acaacgttct cttcgtgtat tggatggtgc ggttaccgtt cttgactcac
    11461 aatcaggtgt tgagcctcaa actgaaacag tttggcgtca agcaactgag tacggagttc
    11521 cacgtatcgt atttgccaac aaaatggaca aaatcggtgc tgacttcctt tactctgtaa
    11581 gcacacttca cgatcgcctt caagcaaatg cacacccaat ccaattgcca atcggttctg
    11641 aagatgactt ccgtggtatc attgacttga tcaagatgaa agctgaaatc tatactaacg
    11701 accttggtac ggatatcctt gaagaagaca tcccagctga ataccttgac caagctcaag
    11761 aataccgtga aaaattgatt gaagcagttg ctgaaactga cgaagaattg atgatgaaat
    11821 acctcgaagg tgaagaaatc actaacgaag aattgaaagc tggtatccgt aaagcgacta
    11881 tcaacgttga attcttccca gtattgtgtg gttcagcctt caaaaacaaa ggtgttcaat
    11941 tgatgcttga tgcggttatc gactaccttc caagtccact tgacatccca gcaatcaaag
    12001 gtattaaccc agatacagac gctgaagaaa ctcgtccagc atctgacgaa gagccatttg
    12061 cagctcttgc cttcaagatc atgactgacc cattcgtagg tcgtttgaca ttcttccgtg
    12121 tttactcagg tgttcttcaa tcaggttcat acgtattgaa tacttctaaa ggtaaacgtg
    12181 aacgtatcgg acgtatcctt caaatgcacg ctaacagccg tcaagaaatc gacactgttt
    12241 actcaggtga tatcgctgct gccgttggtt tgaaagatac tacaactggt gactcattga
    12301 cagatgaaaa agctaaaatc atccttgagt caatcaacgt tccagaacca gttatccaat
    12361 tgatggttga gccaaaatct aaagctgacc aagacaagat gggtatcgcc cttcaaaaat
    12421 tggctgaaga agatccaaca ttccgcgttg aaacaaacgt tgaaactggt gaaacagtta
    12481 tctcaggtat gggtgaactt caccttgacg tccttgttga tcgtatgcgt cgtgagttca
    12541 aagttgaagc gaacgtaggt gctcctcaag tatcttaccg tgaaacattc cgcgcttcta
    12601 ctcaagcacg cggattcttc aaacgtcagt ctggtggtaa aggtcaattc ggtgatgtat
    12661 ggattgaatt tactccaaac gaagaaggta aaggattcga attcgaaaac gcaatcgtcg
    12721 gtggtgtggt tcctcgtgaa tttatcccag cggttgaaaa aggtttggta gaatctatgg
    12781 ctaacggtgt tcttgcaggt tacccaatgg ttgacgttaa agctaagctt tatgatggtt
    12841 catatcacga tgtcgactca tctgaaactg ccttcaagat tgcggcttca ctttccctta
    12901 aagaagctgc taaatcagca caaccagcta tccttgaacc aatgatgctt gtaacaatca
    12961 ctgttccaga agaaaacctt ggtgatgtta tgggtcacgt aactgctcgt cgtggacgtg
    13021 tagatggtat ggaagcacac ggtaacagcc aaatcgttcg tgcttacgtt ccacttgctg
    13081 aaatgttcgg ttacgcaaca gttcttcgtt ctgcatctca aggacgtggt acattcatga
    13141 tggtatttga ccactacgaa gatgtaccta agtcagtaca agaagaaatt attaagaaaa
    13201 ataaaggtga agactaatcc gtcctcactc tagaaggaag tcacttagtg gcttcctttt
    13261 gtctttagaa aatacctcta aatatggtaa aatagtagaa gaataatgtg aggaaaatga
    13321 atgtcaaata gttttgaaat tttgatgaat caattgggga tgcctgctga aatgagacag
    13381 gctcctgctt tagcacaggc caatattgag cgagttgtgg ttcataaaat tagtaaggta
    13441 tgggagtttc atttcgtatt ttctaatatt ttaccgattg aaatcttttt agaattaaag
    13501 aaaggtttga gcgaagaatt ttctaagaca ggcaataaag ctgtttttga aattaaggct
    13561 cggtctcaag aattttcaaa tcagctcttg cagtcctact atagggaagc tttctctgaa
    13621 ggtccatgtg ctagtcaagg ttttaagtcc ctttatcaaa atttgcaagt tcgtgctgag
    13681 ggtaatcagc tatttattga aggatctgaa gcgattgata aggaacattt taagaagaat
    13741 catcttccta atttagccaa acaacttgaa aagtttggtt ttccaacttt taactgtcaa
    13801 gtcgagaaga atgatgtcct gacccaagag caggaagagg cctttcatgc tgaaaatgag
    13861 cagattgttc aagctgccaa tgaggaagcg ctccgtgcta tggaacaact ggagcagatg
    13921 acacctcctc cagcggaaga gaaaccagtc tttgattttc aagcgaaaaa agctgcagct
    13981 aaacccaagc tggataaggc ggagattact cctatgatcg aagtgacgac agaggaaaat
    14041 cgtctggtat ttgaaggggt tgtttttgat gtggagcaaa aagtgactag aacaggtcgt
    14101 gttttaatca actttaaaat gacggactat acttcaagtt tttctatgca aaagtgggtt
    14161 aaaaacgagg aagaggccca gaagtttgac ctcatcaaga agaattcttg gctccgagtt
    14221 cgagggaatg tggagatgaa taacttcaca cgcgatttga ctatgaacgt acaggatctg
    14281 caggaagttg ttcactatga gcggaaggat ttgatgccag aaggtgagcg tcgggttgag
    14341 tttcatgctc atactaacat gtcgactatg gatgctttgc cagaggtcga agagattgtt
    14401 gcaacagctg ctaagtgggg acacaaggcg gttgctatca cagaccatgg gaatgtccag
    14461 tcctttccac atggctataa ggcggctaag aaagcgggaa tccagctgat ctatgggatg
    14521 gaagccaata tcgtggagga ccgtgtccct atcgtctata acgaagtgga gatggactta
    14581 tcagaagcaa cctacgtggt ctttgacgtg gaaacgacgg gactttcagc tatctataat
    14641 gacttgattc aggttgcggc ctctaagatg tacaagggga atgttattgc tgaatttgat
    14701 gaatttatca atcctgggca tcccttgtca gcttttacta cagagttaac tggaattaca
    14761 gatgatcatg tcaaaaatgc caaaccacta gaacaagttt tgcaagaatt ccaagaattt
    14821 tgcaaggata cggtcctagt tgcccacaat gctacctttg acgttggctt tatgaatgct
    14881 aattatgagc gtcatgatct tccaaagatt agtcagccag ttattgatac gctggagttt
    14941 gctagaaacc tctatcctga gtataaacgc catggtttgg ggcctttgac caagcgtttt
    15001 ggtgtggcct tggaacatca ccacatggcc aactacgatg cggaagcgac tggtcgtctg
    15061 cttttcatct ttatcaaaga ggtagcagaa aaacatggtg tgaccgattt agctagactc
    15121 aacattgatc taatcagtcc agattcttac aaaaaagctc ggatcaagca tgcgaccatc
    15181 tatgtcaaga atcaggtagg tctaaaaaat atctttaagc tggtttcctt gtctaatacc
    15241 aagtattttg aaggagtgtc acggattccg agaacggttc tagatgccca tcgagagggc
    15301 ttgattttag gttcagcctg ttcagagggt gaagtttttg acgtggtcgt ttctcaaggt
    15361 gtggatgcgg cggttgaggt ggccaagtat tatgacttta tcgaggtcat gccaccggct
    15421 atctatgcgc ccttgattgc taaagagcag gtcaaggata tggaggaact ccagaccatt
    15481 atcaagagtt tgatagaggt tggagaccgc cttggcaagc ctgttctggc tacgggaaat
    15541 gttcactata tcgaaccgga agaagagatt tatcgtgaaa ttatcgtccg tagtttggga
    15601 cagggtgcga tgattaaccg aactatcggt catggtgaac atgcccaacc agcaccactt
    15661 ccaaaggctc attttcgaac gactaatgag atgttggatg aatttgcctt tttgggagag
    15721 gaactggctc gtaaactggt tattgaaaac accaatgcct tggcagaaat atttgaaccc
    15781 gttgaagtcg ttaagggtga cttgtatacg cctttcatcg acaaggctga agaaacagtt
    15841 gctgagttga cctataagaa agcttttgag atttatggaa atccgctgcc agatattgtt
    15901 gatttgcgga ttgaaaaaga attaacatcc atactgggga atggatttgc tgtgatttat
    15961 ctggcatcgc agatgctggt gcaacgttct aatgaacggg gttatttggt tggttctcgt
    16021 gggtctgtcg gatctagttt cgttgcgacc atgattggga ttacggaggt caatcctctc
    16081 tctcctcact atgtctgtgg tcagtgtcag tacagtgagt ttatcacaga tggttcgtac
    16141 ggctcaggat ttgatatgcc ccataaggac tgtccaaact gtggtcacaa actcagtaaa
    16201 aacggacagg atattccgtt tgagaccttc cttggttttg atggggataa ggttcctgat
    16261 attgacttga acttctcggg agaagatcag cctagcgccc acttggatgt gcgtgatatc
    16321 tttggtgaag aatatgcctt ccgtgcggga acggttggta cggtagctgc caagactgcc
    16381 tatggatttg tcaagggtta cgagcgagat tatggcaagt tttatcgtga tgcagaagta
    16441 gaacgcctcg ctcaaggagc ggcgggtgtc aagcggacaa caggccaaca cccgggggga
    16501 atcgttgtta ttccgaacta tatggatgtc tacgatttta cgcctgtcca gtatccagca
    16561 gatgatgtca cggctgaatg gcagaccact cactttaact tccacgatat cgatgagaac
    16621 gtcctcaaac tcgatgtact ggggcatgat gatccgacca tgattcgaaa acttcaggat
    16681 ttgtctggta ttgaccctaa tgaaattcct atggatgacg aaggcgtgat ggcactcttt
    16741 tctgggactg atgtgctagg ggtaacacct gaacaaattg gaacgcctac gggtatgttg
    16801 gggattccag agtttggaac aaatttcgta cgtggaatgg tagacgaaac ccatccgaca
    16861 acctttgcgg aattgcttca gctgtctggt ctgtcccacg gtactgacgt ttggttgggg
    16921 aatgctcagg atctgattaa gcaaggaata gcggacctat cgactgttat cggttgtcgg
    16981 gacgacatca tggtttacct catgcatgcg ggtctggaac ctaagatggc ctttaccatt
    17041 atggaacggg tacgtaaggg tttgtggcta aagatttcag aagaggagag aaatgactat
    17101 atcgaagcca tgaaggctaa taaggtgcca gagtggtata tcgaatcctg tgggaaaatt
    17161 aagtacatgt tccctaaggc ccatgcggca gcctacgtta tgatggcctt gcgtgtagct
    17221 tacttcaagg ttcaccatcc tatttattac tactgtgctt acttctccat tcgtgctaag
    17281 gcttttgata tcaagaccat gggtgcgggc ttggaggtca tcaagcgcag aatggaagaa
    17341 atctctgaaa aacggaagaa caatgaagcc tctaatgtgg aaatcgatct ctatacaact
    17401 cttgagattg tcaatgagat gtgggaacga ggtttcaagt ttggtaaatt agatctctac
    17461 tgtagtcagg cgacagagtt cctcatcgac ggggataccc ttatcccacc atttgtagca
    17521 atggatggtc tgggagagaa cgttgccaag caactggtgc gggcgcgtga agagggagaa
    17581 ttcctctcta aaacagaact acgcaagcgt ggtggactct catcaacctt ggttgaaaag
    17641 atggatgaga tgggtattct tggaaatatg ccagaggata accagttgag tttgtttgat
    17701 gagttgtttt aaaaaattgc ttaataatct attaaaagag gctaacgtat atccaataga
    17761 tttacattag ctttcttttt tgttaaaata gtctatggaa agagggtgag agtatgtcaa
    17821 agatgagtat aagcatccgt ctggatagtg aggttaagga gcaggcccaa caggtgttta
    17881 gtaatctggg aatggatatg acaacagcta ttaatatttt ccttcgtcag gcaattcaat
    17941 atcagggatt accttttgat gttagactag acgaaaatcg gaagttgctc caagtgttaa
    18001 cggatttaga ccaaaatcgt aatatgagcc agtcttttga atcagtctca gatttgatgg
    18061 aggacttacg tgcttaagat tcgttatcat aaacagttta aaaaagattt taagttggct
    18121 atgaagcgtg gtttgaaggc agaattatta gaagaagttt tgaattttct ggttcaagaa
    18181 aaagaacatc ctgccagata tcgtgatcat tcattgacgg catccaagca ttttcaagga
    18241 gttcgtgaat gccataccca gccagattgg cttttggttt ataaagtaga caagtcggaa
    18301 ttgattttaa atttgctgag gacaggcagt cacagtgatt tattttaatc tattttaagg
    18361 gggttctcat gaaactaaga atatttgcgg aagataagcc ggctaagaag gtatttgaat
    18421 atcaattaga acttgctgat cgtacaattc ttctatcgac agcactcttg tcaggtgcta
    18481 ttgctttagc aggaatcttt tctgctttga gagaaaaata aaaatagaaa agagaaaaca
    18541 gaatggtttt accaaatttt aaagaaaatc tagaaaaata tgcgaaattg ttggttgcga
    18601 acggaattaa cgtgcaacct ggtcacactt tggctctctc tattgatgtg gagcaacgtg
    18661 aattggcaca tctaatcgtg aaagaagctt atgccttggg tgcgcatgag gtcatcgttc
    18721 agtggacaga tgatgtgatt aaccgtgaga aattcctcca tgccccgatg gagcgtttgg
    18781 acaatgtgcc agaatacaag attgctgaga tgaactatct cttggagaat aaggctagcc
    18841 gtcttggagt tcgttcatct gatccaggtg ccttgaacgg agtggacgct gacaagcttt
    18901 cagcttctgc taaagctatg ggacttgcca tgaagcctat gcgtatcgca actcaatcta
    18961 acaaggttag ctggactgta gcagctgcag caggacttga gtgggctaag aaagtcttcc
    19021 caaatgctgc gagcgacgaa gaagcagttg atttcctttg ggaccaaatt ttcaaaactt
    19081 gccgtgtcta cgaagcagat cctgttaagg cctgggaaga gcatgcagct atcttgaaga
    19141 gcaaggccga tatgcttaat aaggagcaat tttcagccct tcactacaca gcgccaggaa
    19201 cagatttaac acttggtttg ccaaagaacc acgtttggga atcagctggt gctgtcaatg
    19261 cacagggcga aggattcttg ccaaatatgc cgacagaaga ggtcttcaca gcgcctgact
    19321 tccgtcgtgc agatggttat gtcacttcta caaaaccgct tagctacaac ggaaatatca
    19381 tcgaaggtat taaggtaacc tttaaggatg gacaaatcgt agatatcact gctgagaagg
    19441 gtgatcaggt tatgaaagac cttgtctttg aaaatgcggg tgcgcgtgcc ttgggtgaat
    19501 gtgccttggt accagatcca agtccaattt ctcagtcagg cattaccttc tttaacaccc
    19561 ttttcgatga aaatgcgtca aaccacttgg ctatcggtgc agcctatgcg actagcgtcg
    19621 ttggtggagc agagatgagt gaagaagagc ttgaagctgc gggacttaac cgttcagatg
    19681 ttcacgtgga ttttatgatt gggtctaatc agatggatat cgatggtatc cgtgaggatg
    19741 gaactcgagt acctcttttc cgtaatggga attgggcaaa ttaaggagat aatatgttag
    19801 gaagtatgtt cgttggtctc ctagtgggat ttttagcagg tgctatgacc aatcgtggag
    19861 agcgaatggg atgttttgga aaaatgtttc tcggttggat cggagccttt ctaggtcact
    19921 tgctctttgg aacttggggg ccagttttat caggaacagc tattatccca gctgttttag
    19981 gtgccatgat tgtcttagct attttttgga gacgaggaag ttaatttcct aaatctgata
    20041 ctcaatgaaa atcaaagagc aaactaggaa actagccgca ggttgctcaa agcactgctt
    20101 tgaggttgca gatagaactg acgaagtcag taacatatat acggcaaggc gacgttgacg
    20161 cggtttgaag agatttttga agagtatgaa acgaaaagga ggttggtcat tgtactagcc
    20221 tccttttgat atgatataat agttctatga gattagataa atttttagtt gcctgtgcgg
    20281 tgggaagtcg gactgaggtc aaaaacttgc tcaaggctgg gcgcgtgact gtaaatggta
    20341 aaaaagaaaa atcagctaaa ttgcagattg atgaaaaaat agatgagatt cgctttgatg
    20401 ggcaagtgtt ggagtatgaa gagtttgtct actacatgat gaacaagccc aaaggagtta
    20461 tctcagcgac tgaggatccc aagcacagaa ccgttctgga cttgttggat gacttggcgc
    20521 ggagtaagga agttttccca gtaggacgct tggatattga cacgcatggt cttttgctct
    20581 tgaccaatga tggtcagctg gctcatgttc ttctttcgcc caagcgtcat gtggacaaga
    20641 cttatctggc acaagtcaag ggaatcatga cccaagaaga tgtggagaca tttgctgagg
    20701 gtattcctct caaagacttt acctgtcaac ccgctatact ggagcttgta tccatagata
    20761 cagaaaagaa tcaaagccaa atccgtgtga ccattgcaga agggaagttt catcagatca
    20821 agcgtatggt gggctactgt ggcaaggaag tggtggactt gcaacgattg actatgggaa
    20881 ctttagtatt agatgagaac ctggaacgag gggaatggcg tcgcttgacc aaggaagaat
    20941 tagaaattct ccgcgctaat attatttaat ttagt
//
LOCUS       DBERDK010000032        20920 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.032, whole genome shotgun sequence.
ACCESSION   DBERDK010000032 DBERDK010000000
VERSION     DBERDK010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 20920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20920
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.032"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            69..566
                     /locus_tag="ACL73L_001170"
     CDS             69..566
                     /locus_tag="ACL73L_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HGS0772156.1"
                     /translation="MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPN
                     TVFAGFYLFDGKELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYIS
                     CDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDAMDRDYLEQFVAILLEKTTWDFTM
                     FEEKS"
     gene            566..2221
                     /gene="dnaX"
                     /locus_tag="ACL73L_001171"
     CDS             566..2221
                     /gene="dnaX"
                     /locus_tag="ACL73L_001171"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HGS0772157.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYAPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIKEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTAISEEITGTISLSALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTDLLHYLRDLLIVQTGGENTHHSSVFVENLALPQKNLFEMIRLATVSLADIK
                     SSLQPKIYAEMMTVRLAEIKPEPALSGAVENEIATLRQEVARLKQELSNVGAVPKQVA
                     PALSRPATGKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLAGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQTEKEVEESLIPEGFEFLADKVKVEED"
     gene            2249..2443
                     /locus_tag="ACL73L_001172"
     CDS             2249..2443
                     /locus_tag="ACL73L_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HGS0772158.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGRYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
     gene            2593..3363
                     /gene="sufC"
                     /locus_tag="ACL73L_001173"
     CDS             2593..3363
                     /gene="sufC"
                     /locus_tag="ACL73L_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HGS0772159.1"
                     /translation="MSVLEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEDDEKISVREFITKLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            3391..4653
                     /gene="sufD"
                     /locus_tag="ACL73L_001174"
     CDS             3391..4653
                     /gene="sufD"
                     /locus_tag="ACL73L_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HGS0772160.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIETLELPVIERVKFY
                     RWNLGDGTITENEPSANVPDFTALDHHLKLVQVGTQTVFEQTPVELAEQGVVFTDFHS
                     ALEEIPELIEEFFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEITEPIEGIFYQDS
                     DSNVPFNKHIMIIAGKNSKISYLERLESRGEGSDKATANITVEVIARSGAQVKFAAID
                     RLGENVTAYISRRGKLGNDASIDWAIGVMNEGNVVADFDSDLIGNGSHADLKVVALSS
                     GRQVQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGF
                     LGSVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            4664..5890
                     /locus_tag="ACL73L_001175"
     CDS             4664..5890
                     /locus_tag="ACL73L_001175"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HGS0772161.1"
                     /translation="MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKANNS
                     YYEQDNANVHRGVHTLAERATASYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVAR
                     FAEEILTEGDQVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMEDLRAKLTD
                     KVKFVSLAHASNVLGVVNPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFA
                     FSGHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQSASWKELPWKFEAGTPN
                     MAGAIGLATAVDYLEKIGMDAIEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVI
                     AFNLGDLHPHDLATALDYEGVAVRAGHHCAQPLLQYLEVPATARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            5877..6317
                     /gene="sufU"
                     /locus_tag="ACL73L_001176"
     CDS             5877..6317
                     /gene="sufU"
                     /locus_tag="ACL73L_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HGS0772162.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAEQISLNNPTCGDVI
                     NLSVKFDAEDRLEDIAFLNSGCTISTASASMMTDAVLGKTKQEILELATIFSEMVQGQ
                     KDERQDQLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            6367..7779
                     /gene="sufB"
                     /locus_tag="ACL73L_001177"
     CDS             6367..7779
                     /gene="sufB"
                     /locus_tag="ACL73L_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HGS0772163.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSA
                     AKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFDDLIYYQKPSDKPARSWDDVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
                     LFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFE
                     RTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(8010..9251)
                     /gene="pbp3"
                     /locus_tag="ACL73L_001178"
     CDS             complement(8010..9251)
                     /gene="pbp3"
                     /locus_tag="ACL73L_001178"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase PBP3"
                     /protein_id="HGS0772164.1"
                     /translation="MKKIFLTLLTVSLLGGASTAVAQDFTIAAKHAIAVEANTGKILY
                     EKDATQPVEIASITKLITVYLVYEALENGSITLSTPVDISDYPYQLTTNSEASNIPME
                     ARNYTVEELLEATLVSSANSAAIALAEKIAGSEKDFVDMMRAKLLEWGIQDATVVNTT
                     GLNNETLGDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFAGMTI
                     TSTNYMLEGMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNADHQDNNPY
                     ARFTATSSLMDYISSTFTLRKIVQQGDAYQDSKAPVQDGKEDTVTAVAPEDIYLIERV
                     GNQSSQSVQFTPDSKAIPAPLEAGTVVGHLTYEDKNLIGQGYITTERPSFEMVADKKI
                     EKAFFLKVWWNQFVRFVNEKL"
     gene            9354..10463
                     /gene="cozE"
                     /locus_tag="ACL73L_001179"
     CDS             9354..10463
                     /gene="cozE"
                     /locus_tag="ACL73L_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell elongation protein CozE"
                     /protein_id="HGS0772165.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVSVLNTIMIP
                     FLLGGFLYYLTNPIVTFLNKVCKLNRLLGILITLCTLVWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRVQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDRLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYALVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLIAVIYMLVVQQVDGNILYPRIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSRLYENHKIMKERERELAK"
     gene            11109..11456
                     /gene="ssrA"
                     /locus_tag="ACL73L_001180"
     tmRNA           11109..11456
                     /gene="ssrA"
                     /locus_tag="ACL73L_001180"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(11628..11715)
                     /locus_tag="ACL73L_001181"
     tRNA            complement(11628..11715)
                     /locus_tag="ACL73L_001181"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11679..11681),aa:Ser,seq:gga)
     gene            11877..12617
                     /locus_tag="ACL73L_001182"
     CDS             11877..12617
                     /locus_tag="ACL73L_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGS0772166.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     AENKLRRVHGGAELPHSLQEEETIQEKSVKNLQEKKLLAQKAVSLIKEKDVIFIDAGT
                     TTAFLIHELVNKNVTVVTNSIHHAAQLVEKQIPTVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGMNGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQVIKEKTEVIEV"
     gene            12614..13525
                     /gene="pfkB"
                     /locus_tag="ACL73L_001183"
     CDS             12614..13525
                     /gene="pfkB"
                     /locus_tag="ACL73L_001183"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HGS0772167.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLNIPNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEIN
                     GTGPTVELVQLEELKAILSSLTAEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGALLVTSEGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDAVEAFKWGVACG
                     TATTFSDDLATAELIKETYGKVEVEKR"
     gene            13522..15474
                     /locus_tag="ACL73L_001184"
     CDS             13522..15474
                     /locus_tag="ACL73L_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HGS0772168.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIDEMIKNLTDHGYVTDFETF
                     KEGILAREALTSTGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALAELSQYLMKDGFADKLRQATSADQVIELFDQASEKTEELVQAP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAEMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKAVEMDRFDGKPLINRPVADGIRKTEELINLALSGDTEVYRAANGAK
                     AATASNEKQSLGGAFYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALSVPNENLGNLG
                     SYHELASMFMKIGGAAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFGKIP
                     YAAGGEATSTLAGVSSGFLGALVGGFIAGALVLAIKKYVKVPRSLEGAKSILLLPLLG
                     TILTGFVMLAVNIPMAAINTAMNDFLGSLGGGSAVLLGIVLGGMMAVDMGGPVNKAAY
                     VFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTNIIM
                     GLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALTSNA
                     LLYLVSVLVGAIVSGVVYGYLRKPQA"
     gene            15611..17863
                     /locus_tag="ACL73L_001185"
     CDS             15611..17863
                     /locus_tag="ACL73L_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HGS0772169.1"
                     /translation="MERKEAIQRMLISLGIAILLIFAAFKLGAAGITLYNLIRLLVGS
                     LAYLAIFGLLIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSVLKGT
                     MAQVVTDLTGFRTTSFAGGGLIGVALYIPTAFLFSNIGTYFIGSILILVGSLLVSPWS
                     VYDIAEFFSRGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKALLDLPP
                     VDMETGEILTEEAVQNLPPIPEEKWVEPEIILSQAELKFPEQEDDSDDEDVQVDFSAK
                     EALKYKLPSLQLFAPDKPKDQSKEKKIVRENIKILEATFASFGIKVTVERAEIGPSVT
                     KYEVKPAVGVRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFR
                     ELWEQSQTKAENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASI
                     LMKARPDQVKFMMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYEL
                     FAKVGVRNIAGFNAKVEEFNSQSEYKQIPLPFIVVIVDELADLMMVASKEVEDAIIRL
                     GQKARAAGIHMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLL
                     GRGDMLFKPIDENHPVRLQGSFISDDDVERIVNFIKTQADADYDESFDPGEVSENEGE
                     FSDGDAGGDPLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEMAGVIGPAE
                     GTKPRKVLQQ"
     gene            complement(17906..18337)
                     /locus_tag="ACL73L_001186"
     CDS             complement(17906..18337)
                     /locus_tag="ACL73L_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6556 family protein"
                     /protein_id="HGS0772170.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTITTIGSILGLITAGITIMN
                     ALDNNNKNTKKEPTTSQTTTFVKEIQKESPQENTTPNKENNTSQEKTQQEETPKSSVK
                     EEKKEDQKTATQDSTTPATSKPATENEKQPNTPTSENKSNQ"
     gene            18475..19617
                     /locus_tag="ACL73L_001187"
     CDS             18475..19617
                     /locus_tag="ACL73L_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HGS0772171.1"
                     /translation="MIYFDNSATTKPYPEALETYMQVASKILGNPSSLHRLGDQATRI
                     LDASRQQIADLIGKKIDEIFFTSGGTEGDNWVIKGVAFEKAQFGKHIIVSAIEHPAVK
                     ESALWLKSQGFEVDFAPVDKKGLVDVEALAGLIRPDTILVSIMAVNNEIGSIQPIEAI
                     SEFLADKPTISFHVDAVQALAKIPTEKYLTERVDCATFSSHKFHGVRGVGFVYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIHQALL
                     NYPDIFVFSDEEDFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGT
                     LIAMGVDKDKAKSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            19626..20840
                     /gene="thiI"
                     /locus_tag="ACL73L_001188"
     CDS             19626..20840
                     /gene="thiI"
                     /locus_tag="ACL73L_001188"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HGS0772172.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYTQVKVT
                     ADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSSVQEIMRDIYKE
                     GMTFKISSKRSDHNFELDSRELNQTLGGAVFEAIPNVQVQMKSPDINLQVEIREEAAY
                     LSYETIRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAE
                     KDEVDDLIDNLL"
BASE COUNT         6133 a         3880 c         4642 g         6265 t
ORIGIN      
        1 aaaatccttt gtttactaaa caagggattt ttcttttgtc tctgctcctt ttttgatata
       61 atagttctat gttaaaatca gaaaaacaat cacgttatca aatgttaaat gaagaattgt
      121 ccttcctatt ggaaggcgaa accaatgttt tggctaatct ttccaacgcc agtgctctta
      181 taaaatcacg ttttcctaat accgtatttg caggctttta tttgttcgat ggaaaggaat
      241 tggttttagg tcccttccaa ggaggtgttt cctgcatccg tattgcacta ggcaagggtg
      301 tttgtggtga ggcagctcac tttcaggaaa ctgttattgt tggagatgtg acgacctatc
      361 tcaactatat ttcttgtgat agtctagcta aaagtgaaat tgtggtgccg atgatgaaga
      421 atggtcagtt acttggagtt ctggatctgg attcttcaga gattgaggat tacgatgcta
      481 tggatcgaga ttatttggaa caatttgtcg ctattttgct tgaaaagaca acatgggact
      541 ttacgatgtt tgaggaaaaa tcttaatgta tcaagcactt tatcgaaaat atagaagtca
      601 aaacttctcc cagttagttg gtcaagaagt tgtggctaag actcttaaac aagcggtgga
      661 gcaagagaaa ataagtcacg cttatctttt ttctggtcct cgtggaacgg gaaaaaccag
      721 tgttgctaaa atctttgcca aggctatgaa ctgtcccaat caagtgggtg gcgaaccttg
      781 caataactgc tatatttgtc aagcagtgac ggacggtagt ttagaagatg tcattgaaat
      841 ggatgcagct tctaataatg gggtagatga aattcgcgaa attcgtgata aatctaccta
      901 tgcacctagc cttgctcgtt ataaggttta tatcatagat gaggttcaca tgctgtctac
      961 aggggctttt aatgccctcc taaagacgct ggaagaacca acacagaatg tagtctttat
     1021 tttggccact actgaattgc acaagattcc tgctactatt ctatcccgtg tgcaacgttt
     1081 tgagtttaaa tcaattaaga cacaggatat taaggaacat attcactata tcttagaaaa
     1141 agaaaatatc agttctgaac cagaggctgt ggaaatcatt gccagacggg cggaaggtgg
     1201 aatgcgggac gccttgtcta ttttggatca agccctgagt ttgacacagg gaaatgagct
     1261 gacgactgct atctctgaag aaattactgg caccattagc ctatcagcct tggatgatta
     1321 tgtggcggcc ttgtctcaac aggatgttcc caaagctttg tcttgcttga atcttctttt
     1381 tgacaatggt aagagcatga ctcgttttgt gaccgatctt ttgcactatt taagagactt
     1441 gttaattgtt caaacagggg gagaaaatac tcatcatagt tcagtctttg tagaaaattt
     1501 ggcacttcct caaaaaaatc tgtttgaaat gattcgctta gcaacagtga gtttagcaga
     1561 tattaagtct agtttgcagc ccaagattta tgctgaaatg atgaccgtcc gtttggcgga
     1621 aatcaagccc gaaccagctc tatcaggagc ggttgaaaat gaaattgcta cgctgagaca
     1681 ggaagttgcc cgtctcaaac aagagctttc taatgtaggt gcggttccta aacaagttgc
     1741 accagctctt agtcgaccag ctacgggcaa aacagtctat cgtgtcgatc gcaataaagt
     1801 gcaatctatc ttacaagagg ccgtcgaaaa tcctgattta gcacgtcaaa atttaattcg
     1861 tttgcagaat gcctggggag aggtaattga aagtctaggt gggccggaca aggctctgct
     1921 agctggttct caaccggttg ctgccaatga acaccatgct attcttgctt ttgagtctaa
     1981 cttcaatgct ggtcaaacta tgaaacgaga caatctcaat accatgtttg gtaatatcct
     2041 cagtcaggcg gcaggttttt cacctgagat tttagctatt tccatggagg aatggaaaga
     2101 agttcgcgca gccttttcag ccaaagccaa atcttctcaa actgaaaaag aagtagaaga
     2161 aagcctgatt ccagaaggat ttgaattttt ggctgataaa gtgaaggtag aggaagacta
     2221 aagaaagatt tcatgataca ataagtttat gaataaacaa caatttatta ttatggcgct
     2281 gtttacagct gctgagacct attttttcaa tgaagcctgg atgactggcc gctatattat
     2341 ggcagccttt tgggcaattt tactctttag aaatttccga gtcagttatg tgatgggcaa
     2401 aatcgttgat gtcatcgatc agcattttaa taggaaagac tagccctcag cttccagaca
     2461 aaatcaaagc cttttaggct tttttttgtt atactagaaa agtatattta tagaattttt
     2521 gctctatttc tggggaaatc agacgttttt ctagtaagta ctgtaaaagt tttgaaaaag
     2581 aaaggaacta tcatgtcagt attagagatc aaagatcttc acgttgagat tgaaggaaaa
     2641 gaaattttaa aaggggttaa cctgaccctg aaaacaggag aaattgccgc tatcatggga
     2701 ccaaatggta caggtaaatc gactctttct gccgctatca tgggaaatcc aaactatgaa
     2761 gtaactaaag gtgaagtttt gtttgatggc gtaaacatcc ttgagttgga agtggatgag
     2821 cgtgcgcgta tgggactttt ccttgctatg caatacccat cagaaatccc tggaattacc
     2881 aatgctgagt ttcttcgtgc cgctatgaat gcgggtaaag aagatgatga gaagatttca
     2941 gttcgtgagt ttattactaa gctagatgaa aaaatggaat tgctcaacat gaaagaagaa
     3001 atggcagagc gttacctcaa cgaaggcttc tctggtggtg agaaaaaacg caatgaaatt
     3061 cttcaacttt tgatgttgga gccaacattt gctcttttgg acgagattga ctcaggtctt
     3121 gatattgacg ctcttaaagt tgtgtctaaa ggtgtcaatg ccatgcgtgg tgaaggtttt
     3181 ggtgctatga tcatcactca ctaccaacgt cttttgaact atatcacacc tgatgtggta
     3241 cacgtgatga tggaaggtcg tgttgtcctt tctggtggtc cagaattggc tgcgcgtttg
     3301 gaacgtgaag gatacgcaaa attagctgaa gaacttggct acgactacaa ggaagaattg
     3361 taatttcctc gtatctttta ggagaagtaa atgactagag aaaatattaa acttttttca
     3421 gaaatgcacg ctgaaccaag ctggttggct gatctccgtc aaaaagcttt tgacaagatt
     3481 gagactttgg aattaccagt tattgagcgt gtcaaattct accgttggaa tctgggtgat
     3541 ggaacgatta cagaaaatga gccatcagca aatgttccag atttcacagc tttagatcat
     3601 cacttgaagt tggtgcaagt aggaactcaa actgttttcg aacaaactcc agttgagtta
     3661 gctgaacagg gtgttgtctt cacagacttt cactcagctt tagaagaaat tccagagctg
     3721 atcgaagaat tcttcatgtc atctgttaag tatgatgatg acaagttggc ggcttaccac
     3781 acagcttact ttaacagtgg tgctgtactc tatattccag ataatgtaga aattacagag
     3841 ccaattgaag gaattttcta ccaagatagc gatagcaatg tgccgtttaa caagcatatt
     3901 atgattatcg ctggtaaaaa ttctaagatt agttatctgg agcgtttaga gtcacgcggt
     3961 gaaggaagtg acaaagcaac tgccaatatc acagtggaag tgattgcacg ttctggtgcg
     4021 caagtcaagt ttgctgctat cgaccgtcta ggtgaaaacg tcactgccta cattagccgt
     4081 cgtggtaaat taggcaacga tgcaagtatt gactgggcta tcggtgtcat gaacgaagga
     4141 aatgtcgttg ctgattttga tagtgacttg attggtaatg gtagccatgc tgacctcaag
     4201 gttgtagctc tttcaagtgg tcgtcaggta caagggattg atactcgtgt aactaactat
     4261 ggctgcaact caatcggaaa cattctacaa catggggtta tccttgaaaa agcaactttg
     4321 actttcaatg gtatcggcca catcatcaag ggtgctaagg gagcagatgc gcaacaagag
     4381 agccgtgttc tcatgctttc agaccaagcg cgttcagatg ctaacccaat tcttttgatt
     4441 gatgaaaatg acgtaactgc aggacatgca gcctctattg gtcaggtaga tccagaagac
     4501 atgtattacc tcatgagtcg tggattggat aaggcaactg cagagcgttt ggttgttcgt
     4561 ggtttccttg gatctgttat cgtggagatt ccagtcaagg aagttcgtga tgaaatgatt
     4621 gcaactatcg aagagaaatt gtcaaaacgc taaggggaag cctatgttag atgtagaagc
     4681 gattcgcaag gattttccaa ttttagatca gattgtcaat gatgaacctc tggtctatct
     4741 ggacaatgct gcgacgacac aaaaaccact agtagttctg aaagctaata acagctacta
     4801 tgagcaggac aatgccaatg ttcaccgtgg tgtccatacc ttagcggaac gagcgacagc
     4861 ttcttatgaa gctgctcgtg aaaccattcg taagtttatt aatgcaggct ctacaaagga
     4921 agttctcttt accagaggaa cgacaaccag ccttaactgg gtggcacgct ttgctgagga
     4981 aattctcact gagggagacc aggtcttgat ttcagtaatg gaacaccatt ctaatatcat
     5041 tccatggcag gaagcttgtc gaaagactgg agcagagctt gtctatgtct atcttaaaga
     5101 cggtgccttg gatatggagg atttgcgagc taaattgact gataaggtta aatttgtttc
     5161 cctagctcat gcctccaatg ttcttggtgt ggtcaatccg atcaaggaaa tcactcaatt
     5221 agcccaccaa gttggggcaa tcatggtagt ggatggtgct caatctacac ctcatatgaa
     5281 gattgatgtc caggacttgg atctggactt tttcgccttt tcgggtcaca agatggctgg
     5341 tccgactggt atcggtgtcc tttacggcaa agaaaagtat cttgagcaaa tgtctccagt
     5401 agaatttggc ggcgagatga ttgattttgt ctacgagcaa tctgctagtt ggaaggaatt
     5461 gccttggaaa tttgaggctg gaacgccaaa tatggcagga gctattggac ttgcgactgc
     5521 agttgattat ctggaaaaga ttggtatgga tgccattgaa gctcatgaac aggaattgat
     5581 tgcgtacgtc tatccaaaac tgcaggcaat tgagggattg accatttacg gttctcagga
     5641 tttggctcaa cgttcgggtg ttattgcctt taacctaggt gatctccatc ctcacgatct
     5701 tgcgacggct ctggattatg aaggagtggc tgttcgtgct ggtcaccatt gtgcgcaacc
     5761 cttgcttcag tatttggaag tcccagcaac agctcgtgca agtttttata tctacaatac
     5821 caaggcagat tgcgacaaac tagtcgatgc cctacaaaag acaaaggagt ttttcaatgg
     5881 cactttctaa actagatagc ctttatatgg cagtggtagc agaccattcg aaaaatccac
     5941 atcaccaagg gaagttagaa gacgctgagc aaatcagtct caacaatccg acttgtgggg
     6001 atgtcatcaa cctctctgtc aagtttgatg cagaggaccg tttggaagat attgcttttc
     6061 taaattcagg atgcacgatt tcaactgctt ctgctagtat gatgacagat gccgttttag
     6121 gaaaaaccaa acaagaaatt ttagaactgg cgactatttt ttctgaaatg gttcaagggc
     6181 aaaaagatga gcgtcaagac caacttggag acgcggcatt cttgtcaggt gttgccaaat
     6241 tccctcaaag aatcaagtgt gcaaccctag cttggaatgc ccttaagaaa acaattgaaa
     6301 atcaagaaaa acagtaagac aagtttcttt tgtcttatga attattagaa atgaagaaag
     6361 gatattatgg ctgaagaaag agtagaacca aaaccaattg accttggtga atataaattt
     6421 ggtttccatg acgatgtaga gcctgtctta tcgacaggaa aaggactcaa cgaaggtgtt
     6481 attcgtgaat tatctgctgc taagggtgag cctgagtgga tgttggagtt ccgtttgaag
     6541 tcttatgaaa ccttcaaaaa aatgcccatg caaacttggg gagcagactt gtcagagatt
     6601 gactttgatg acttaatcta ctaccaaaaa ccatctgaca aaccagcccg ttcttgggat
     6661 gatgtacctg aaaagattaa agaaaccttt gaacgtatcg ggattccaga agctgaacgt
     6721 gcttatttag caggggcttc tgcccagtac gagtcagaag tggtttacca caacatgaag
     6781 gaagagttcc aaaaattagg tattatcttt acagatacag attccgcact caaggaatac
     6841 ccagacttat ttaaacaata ctttgcgaag ttggtaccgc cgacagataa caagttggca
     6901 gccctcaact cagcagtatg gtcgggtgga acttttatct acgtgccaaa aggtgtcaag
     6961 gtagatattc cacttcaaac ttatttccgt atcaataacg aaaatatagg tcagttcgaa
     7021 cgtaccttga ttatcgttga tgagggagca agcgtccact acgtagaagg atgtacagca
     7081 ccaacatatt caagcaatag cttacacgct gccattgtag aaatttttgc tttggacgga
     7141 gcttatatgc gttatacaac tatccaaaac tggtctgata acgtctataa cttggtaaca
     7201 aagcgtgcta aggctcaaaa ggatgccact gttgagtgga ttgatggaaa cttgggtgcc
     7261 aaaacgacta tgaaatatcc atctgtttac cttgatggag aaggagcgcg tggtaccatg
     7321 ctctctatcg cctttgctaa tgcagggcaa caccaagaca cgggtgctaa gatgattcac
     7381 aatgctccac ataccagctc gtctattgtg tctaaatcca tcgctaaagg tggaggaaag
     7441 gttgactacc gtggacaagt cacctttaac aagaactcta agaaatctgt ttcccacatt
     7501 gaatgtgata ccattatcat ggatgacttg tcagcatcag atactattcc atttaatgaa
     7561 attcacaact cgcaagtggc tttggaacac gaagccaaag tatctaagat ttcagaagag
     7621 caattgtatt atctcatgag ccgtggattg tcagaatctg aggcaactga aatgattgtc
     7681 atgggatttg tagaaccctt tacaaaagaa cttccaatgg aatacgcagt tgagctgaac
     7741 cgcttgatta gctatgaaat ggagggatca gttggataaa atttgatttt atactcttcg
     7801 aaaatctctt caaaccacgt cagcatcgcc ttaccgtatg tatggttact gacttcgtca
     7861 gtttcatcta caacctcaaa acagtgtttt gagcaacctg cggctagctt cctagtttgt
     7921 tctttgattt tgagtattag atttactcaa aatcaaggat tttgaagatg aacttgtatc
     7981 aaaaaatcgc ggtttaaaat cgcgattttt tataatttct cgttaacaaa gcggacaaac
     8041 tgattccacc aaacttttaa gaagaaggct ttttcaattt tcttgtctgc taccatttcg
     8101 aaactagggc gctctgtggt gatgtaacct tgaccaatca agttcttgtc ttcataagtc
     8161 aaatggccaa ccactgttcc agcttcaagt ggtgctggga ttgctttgga atcaggtgtg
     8221 aattgaacag attgggaaga ttgattccca acacgttcta ttagatagat atcctctgga
     8281 gccactgcag ttactgtatc ttcttttcca tcttgtacag gggctttgct atcttgatag
     8341 gcatcgcctt gttgaacgat tttgcgaagt gtaaatgtag aagaaatata atccattagg
     8401 gaagatgtag ctgtaaatcg agcgtaagga ttattgtctt gatgatctgc atttaaaaca
     8461 actgtgatga ctctcatgcc tttttcgaca gtagtaccaa caaaagactc tccagcctta
     8521 tctgttgttc ctgtttttag cccatcaaaa ccaccacggt aagcaggcat accttctaac
     8581 atgtagttgg ttgaagtgat tgtcatccca gcaaaagtag aagaaggttt tttggtgatt
     8641 tctaagactt gtgggtattt tttgatgagg ttgcgagcaa cgatagcgac atcataagca
     8701 ctaagcttat tttcctcatc ttttttagaa cctgggtaaa tgttatcccc tagagtttca
     8761 ttgttaagac ctgtcgtatt gacaacagtg gcatcctgaa ttccccattc caagagtttt
     8821 gcccgcatca tatcgacgaa atctttttct gagccagcaa ttttctcagc tagggcaata
     8881 gcggcgctgt tggcactaga taccagagtt gcttcaagca actcttcgac agtataatta
     8941 cgggcctcca taggaatatt actggcttca gaatttgtcg tcaattgata aggataatca
     9001 gaaatatcta caggagtgga gagggtaata cttccgtttt ccaaagcttc atagaccaga
     9061 taaacagtaa tcaattttgt tatggaagca atttcgacag gttgcgttgc atccttctca
     9121 tagagaattt taccagtatt tgcctcaaca gcaatcgcat gtttagcggc aatggtaaaa
     9181 tcttgagcaa cagcagtaga agcaccccct aaaagagaga cagttaacaa agttaaaaat
     9241 atttttttca tagtagtttt attctatcat aaagaaaaaa aatattcttg ctttaataat
     9301 tcatctgtta agctttttga aaatatggta aaataaagtg agggaggtaa ctcatgtttc
     9361 gtagaaataa attatttttt tggaccacag aaattttact cttaaccatc atcttttacc
     9421 tatggagaca gatggggtct ttgattaacc cttttgttag cgtgcttaat acaattatga
     9481 ttccattttt attagggggc tttctttatt atttgacaaa ccctattgtt actttcttaa
     9541 ataaagtctg taaactcaat cgtttgcttg gtattttaat taccttgtgt actttggtct
     9601 ggggaatggt cataggtgtt gtctatctct tacctatttt gattaatcag ttatctagtt
     9661 tgattatatc tagtcaaact atttatagtc gagtacaaga cttaatcata gacttatcta
     9721 attatcctgc gctccagaat ttggatgtag aagctacaat tcagcagtta aacttatcct
     9781 atgttgatat tcttcaaaat atcctaaata gcgtatcaaa tagtgtgggg agcgtcttgt
     9841 cagctcttat cagtactgtt ttgattttga ttatgactcc agtttttttg gtttatttct
     9901 tattagatgg acataaattc ttgcccatgc ttgaaagaac gattctaaag agggatcgct
     9961 tgcatattgc aggcttatta aagaatttaa atgcgacgat tgctcgctat attagtggag
    10021 tttcgattga cgcaatcatt ataggttgtt tggcttatat tggctatagt attattggtt
    10081 taaaatatgc tttagttttt gccatttttt ctggtgtagc caatttaatt ccttatgtgg
    10141 ggccaagtat tggtttgatt cctatgatca tcgcaaatat attcactgat ccccatagac
    10201 tgctgattgc agtgatttat atgcttgttg ttcagcaggt agatggcaat atcttatatc
    10261 ctcgaatcgt aggaagtgtt atgaaggttc atccaatcac gattttagtt ttacttttgt
    10321 tgtcaagcaa tatctatggt gtagttggaa tgattgtcgc agtgccaacc tattctatct
    10381 tgaaagaaat ttctaagttc ttatcccgtt tgtatgaaaa tcataaaata atgaaagaac
    10441 gagaaagaga attagctaag taaaagtcag gagaaccctg atttttcttt actggaagtg
    10501 gcctttagat tagaagactg aaaataagtt aaagtcttaa actaattttc acagctaaga
    10561 atagtagaag ttaatctgat aaaaatcgaa aaaaccagtg gaattccgtg tcagggtaag
    10621 ttccactggt tttcatagtc tattaaagtt cgaatgaaac ctatttatag tatattgaaa
    10681 ctagaatagt acacctctaa ttctaaaaca ttgttagaaa tcgatttgac tgtcctaatc
    10741 tattcgtcct attcttattt cattttacta tattttggtg caataagtga aaagtagtcc
    10801 gaataatata aggattgatt ttatagtttt taaactcaaa tgaattaaaa taaagagagt
    10861 acgaaaattc tcatctgaaa gtattttaga ataattctct tcgtgaattt cttcaaaaca
    10921 gatagcttca tcttaggtat gtgttaattg gatttcgtaa gctctattta caatcgtaaa
    10981 gttatgcact gagaaagttg tagttagtct tccagtttgc taaatttttc gaatagtatc
    11041 ttttttaggt atgtgatttc tttttgcatt tttgagttag ataaggtata atggttttat
    11101 tgtcttttgg ggtcgttacg gattcgacag gcattatgag gcatattttg cgactcgtgt
    11161 ggcgacgtaa acgctcagtt aaatataact gcaaaaaata acacttctta cgctctagct
    11221 gcttaaaaac cagcaggcgt gacccgattt ggattgctcg tgttcaatga caggtcttat
    11281 tattagcgag atacgattaa gccttgtcta gcggcttgat aagagattga tagactcgca
    11341 gtttctagac ttgagttatg tgtcgagggg ctgttaaaat aatacataac ctatggttgt
    11401 agacaaatat gttggcaggt gtttggacgt gggttcgact cccaccggct ccattattcc
    11461 tttgcattcc ttggtaaaac gttgttaaat caacgttttt tatttttatc tttggtattc
    11521 ctttgcattc ttttgctaaa aagggagtca caaacagacc ctattttaaa aaaggataga
    11581 aaaaaggata caacatttgt cgcatcctaa aaataatctt ttttccacgg aagacatggg
    11641 attcgaaccc acgcacgcta ttacacgcct accgcgtttc caacacggcc tcttaagcct
    11701 cttgagtaat cttccaatac ttactcaaat agtctaccat aaaggctctt atcttgcaat
    11761 aaaaattcta gaaataagaa aaatgataga ttttgaaaga aaatgataaa aaatgcttga
    11821 cttcaaaaga aagtatgata gaatgaatag tgtaaacgat aacaggaggt gattcggtgt
    11881 taaaaacaga gcggaagcaa ctgattttag aggagttaaa tcaacatcat gtagtttctc
    11941 tagaaaaatt agttagtttg ctagaaacgt cagaatcaac ggttcgaaga gacttggatg
    12001 agttggaagc ggaaaacaag cttcgtcgtg tgcatggtgg agcagaactc ccccactcct
    12061 tacaggaaga agaaaccatt caagaaaaat ctgtcaaaaa ccttcaagaa aagaagttgc
    12121 tggctcagaa agcagtctct ctcattaaag aaaaagatgt catctttatc gatgctggaa
    12181 caacaactgc ttttttgatt catgaattgg tcaataagaa tgttacagtt gtgaccaact
    12241 ccattcacca tgccgctcag ttggttgaaa agcagattcc aactgtcatg gttggaggaa
    12301 acgtcaagac ggcgacagat gctagtatcg ggggcgttgc tcttaaccag attaaccaat
    12361 tgcactttga ccgtgccttt atcggaatga atggtgttga cgatggctat tatacgactc
    12421 ctgatatgga ggagggagct gtgaaaagag ctattttgga gaatgccaag cagacctacg
    12481 tcttggtgga ttcgtcaaaa attggacaaa cttgctttgc caaggtagcc ccactcaaac
    12541 gcgctatcgt tatcactagt caagggcatg agctcttgca ggttattaag gagaaaacgg
    12601 aggtaataga agtatgattt atacagtcac actcaatcca tccattgact atatcgttcg
    12661 tttggaccaa gtcaaagttg gtagtgtaaa tcgtatggac agtgatgata agtttgctgg
    12721 tgggaaagga atcaatgtca gccgtgtctt gaaacgtttg aatataccaa atacagcgac
    12781 gggatttatc ggtggcttta ctggtaaatt tatcacagat actttagcag aggaagaaat
    12841 cgagacacgt tttgtccagg tggcagaaga tactcgtatc aatgttaaaa ttaaagcaga
    12901 ccaagaaaca gaaatcaacg gaacgggtcc aactgttgaa ctggttcagc tagaagaatt
    12961 gaaagctatt ttatctagtc tgacagcaga agatacagtt gtctttgcag gttcaagtgc
    13021 taaaaatcta ggtaatgtta tctataagga tttgatttcc ttgacgcgcc agactggtgc
    13081 gcaagtggtc tgtgactttg aaggacagac cttaattgat agtttggact accagcctct
    13141 tcttgtaaaa ccaaacaatc atgaacttgg agcgattttt ggggttaaac tcgaaagttt
    13201 agatgaaatt gagaaatacg ctcgtgagtt actggctaag ggtgctcaaa atgttattat
    13261 ctctatggct ggggacggtg ctcttcttgt cacatctgag ggagcttact ttgctaaacc
    13321 aatcaaagga acagtcaaaa attcagttgg ggctggtgat tctatggttg ctggattcac
    13381 aggtgaattt gtcaaatcaa aagacgcagt agaagccttc aaatggggag tggcttgcgg
    13441 aacggcaact accttctcag atgacttggc aacggcagaa cttattaaag aaacatatgg
    13501 aaaagttgag gtagaaaaac gatgaaaatt caagacctat tgagaaaaga tgtcatgttg
    13561 ctagatttgc aggcaactga aaaaacagct gtcatcgacg agatgattaa aaatttgaca
    13621 gaccacggtt atgtaacaga ttttgaaaca tttaaagaag gaattttggc gcgtgaagct
    13681 ttgacttcta ctggtttggg tgatggaatc gcaatgcctc acagcaaaaa cgctgctgtc
    13741 aaagaagcga cagttctatt tgctaagtca aataagggtg ttgactacga gagcttggat
    13801 ggacaagcaa ctgacctctt cttcatgatt gcagctccag aaggtgccaa tgatactcac
    13861 ttggcagcct tggcagaatt gtctcaatac ttgatgaaag acggttttgc agacaaactt
    13921 cgtcaagcaa catctgcaga ccaagttatc gaactttttg accaagcttc agaaaaaact
    13981 gaggaacttg ttcaagcacc tgctaatgac tctggtgact ttatcgtagc tgttacagct
    14041 tgtacaacag gtattgccca cacttacatg gcccaagaag cccttcaaaa agtagctgct
    14101 gaaatggggg ttggtatcaa ggtcgaaacc aacggtgcta gcggtgttgg aaatcaacta
    14161 actgcagaag atatccgtaa ggctaaagct attatcattg cagcagacaa ggccgttgaa
    14221 atggatcgat ttgatggaaa accattgatc aatcgtccag ttgctgacgg tatccgtaag
    14281 acagaagagc taattaactt ggctctttca ggagatactg aagtctaccg tgccgctaat
    14341 ggtgcaaaag ctgcaacagc ctctaacgaa aaacaaagcc ttggtggtgc cttctacaaa
    14401 cacttgatga gtggtgtatc tcaaatgtta ccattcgtta tcggtggtgg tatcatgatt
    14461 gcccttgcct tcttgattga cggtgctttg agtgttccaa atgaaaacct tggcaatctt
    14521 ggttcttacc atgagttagc ttctatgttc atgaaaattg gtggagctgc ctttggtttg
    14581 atgcttccag tctttgcggg ttatgttgcc tactctattg ctgaaaaacc aggtttggta
    14641 gcaggtttcg tggctggtgc tattgccaaa gaaggttttg cctttggtaa aattccttat
    14701 gccgcaggtg gtgaagcaac ttcaactctt gcaggtgtct catctggttt cctaggtgcc
    14761 cttgttggtg gatttatcgc aggtgccttg gttcttgcca tcaagaaata cgttaaagtt
    14821 cctcgttcac tcgaaggtgc taaatcaatc cttctattgc cacttcttgg aacaatcttg
    14881 acaggatttg ttatgttagc tgtgaatatc ccaatggctg caatcaacac tgctatgaat
    14941 gacttcctag gcagtcttgg aggaggttca gctgtccttc ttggtatcgt ccttggtgga
    15001 atgatggctg ttgacatggg tggaccagtt aataaagcag cttatgtctt tggtacaggt
    15061 acgcttgcag caactgtttc ttcaggtggt tctgtagcca tggcagcagt tatggctgga
    15121 ggaatggtgc caccacttgc aatatttgtc gcaactcttc ttttcaaaga taaatttact
    15181 aaggaagaac gtaactctgg tttgacaaac atcatcatgg gcttgtcatt tatcactgag
    15241 ggagcgattc catttggtgc cgctgaccca gctcgtgcga ttccaagctt catccttggt
    15301 tcagcagtag caggtggact cgttggtctt actggtatca aactcatggc gccacacgga
    15361 ggaatcttcg ttatcgccct tacttcaaat gctctccttt acctcgtttc tgtcttggta
    15421 ggagcaatcg taagtggtgt ggtttatggt tacctacgca aaccacaagc ataaaaaata
    15481 gaaaaatgaa aagattggac cgtttggtgc agtctttttc tcttcccgaa atgcctgtga
    15541 aatatggtat aatagaagaa tggcaaacaa gaatacaagt acaacaagac ggagaccgtc
    15601 taaagcagaa ctggaaagaa aagaagcgat tcaacgaatg ttgatttcgt taggaattgc
    15661 gattttattg attttcgcag ccttcaaatt aggggctgca ggtataaccc tttataattt
    15721 aattcgcttg ctagtgggta gcctagctta tctggcgata ttcggcctat taatctatct
    15781 cttctttttc aagtggatac gaaaacagga aggactctta tctggctttt tcaccatatt
    15841 tgctggctta ctcttgattt ttgaggccta cttggtttgg aaatatggtt tggacaagtc
    15901 cgttctaaaa gggaccatgg ctcaggttgt gacagatctg actggttttc gaacgactag
    15961 ctttgctgga gggggcttga tcggggtcgc tctttatatt ccaacagcct ttctcttttc
    16021 aaatatcgga acttacttta ttggttctat cttgatttta gtgggttctc tcctagtcag
    16081 cccttggtct gtttacgata ttgctgaatt tttcagtaga ggctttgcca aatggtggga
    16141 agggcacgag cgtcgaaaag aggaacgctt tgtcaaacaa gaagaaaaag ctcgccaaaa
    16201 ggctgagaaa gaggctagat tagaacaaga agagactgaa aaagccttac tcgatttgcc
    16261 tcctgttgat atggaaacgg gtgaaattct gacagaggaa gctgttcaaa atcttccacc
    16321 tattccagaa gaaaagtggg tggaaccaga aatcatcctg tctcaagctg aacttaaatt
    16381 ccctgaacag gaagatgact cagatgacga agatgttcag gtcgattttt cagccaaaga
    16441 agcccttaaa tacaaacttc caagcttaca actctttgca ccagataaac caaaagatca
    16501 gtctaaagag aagaaaattg tcagagaaaa tatcaaaatc ttagaagcaa cctttgctag
    16561 ctttggtatt aaggtaacag ttgaacgggc cgaaattggg ccatcagtga ccaagtatga
    16621 agtcaagccg gctgttggtg taagggtcaa ccgcatttcc aatctatcag atgacctcgc
    16681 tctagccttg gctgccaaag atgtccggat tgaagcacca atccctggga aatccctaat
    16741 cggaattgaa gtgcccaact ccgatattgc cactgtatct ttccgagaac tatgggaaca
    16801 atcgcaaacg aaagcagaaa atttcttgga aattccttta gggaaggctg ttaatggaac
    16861 cgcaagagct tttgaccttt ctaaaatgcc ccacttgcta gttgcaggtt caacgggttc
    16921 agggaagtca gtagcagtta acggcattat tgctagcatt ctcatgaagg cgagaccaga
    16981 tcaagttaaa tttatgatgg tcgatcccaa gatggttgag ttatctgttt acaatgatat
    17041 tccccacctc ttgattccag tcgtgaccaa tccacgcaaa gccagcaagg ctctgcaaaa
    17101 ggttgtggat gaaatggaaa accgttatga actctttgcc aaggtgggag ttcggaatat
    17161 tgcaggtttt aatgccaagg tagaagagtt caattcccag tctgagtaca agcaaattcc
    17221 gctaccattc attgtcgtga ttgtggatga gttggctgac ctcatgatgg tggccagcaa
    17281 ggaagtggaa gatgctatca tccgtcttgg gcagaaggcg cgtgctgcag gtatccacat
    17341 gattcttgca actcagcgtc catctgttga tgtcatctct ggtttgatta aggccaatgt
    17401 tccatctcgt gtagcatttg cggtttcatc aggaacagac tcccgtacga ttttggatga
    17461 aaatggagca gaaaaacttc ttggtcgagg agacatgctc tttaaaccga ttgatgaaaa
    17521 tcatccagtt cgtctccaag gctcctttat ctcggatgac gatgttgagc gcattgtgaa
    17581 cttcatcaag actcaggcag atgcagacta cgatgagagt tttgatccag gtgaggtttc
    17641 tgaaaatgaa ggagaatttt cggatggaga tgctggtggt gatccgcttt ttgaagaagc
    17701 taagtctttg gttatcgaaa cacagaaagc cagtgcatcc atgattcagc gtcgtttgtc
    17761 agttggattt aaccgtgcga cccgtctcat ggaagagctt gagatggcag gtgtcattgg
    17821 tccagctgaa ggtaccaagc caagaaaagt attgcagcaa taaaaaatag cttctttcca
    17881 aggttggaag gagctatttt actggctatt gattactttt attttctgaa gttggagtat
    17941 tgggctgttt ttcattttca gtggcaggtt tacttgtagc aggtgtagta gagtcctgag
    18001 ttgctgtttt ctgatcttct tttttctctt ccttgacgct agattttggc gtttcttctt
    18061 gttgtgtttt ttcttgagaa gtgttatttt ccttatttgg agtagtattt tcctgagggg
    18121 attctttttg aatttctttg acaaaggttg tcgtctggct tgtcgtaggt tcttttttag
    18181 tatttttatt attattatcc aaggcgttca tgatcgtgat acccgctgta atcaaaccaa
    18241 ggatactacc gatagtagta atggtttttt gaaaaacagt aaagcctttt ttgatatgtt
    18301 ctgttttcgg ttttgaagtt ctacgatagt tagacataca ttctctcctt tgattatgat
    18361 ttattatact ctgtttcttt aaaaaaatct taaaaaaaga gaaattgccc tttcttgtcg
    18421 caagctccgt ttcatgatac aatagaggga gtgattttag aaagcgtgag aaacatgatt
    18481 tactttgata attcagcgac gaccaagcct tatcctgaag cccttgaaac ctatatgcag
    18541 gtcgcttcaa aaattttagg aaacccatct agtctccatc gtttgggaga ccaagcaaca
    18601 cgaatcttag atgcttctcg ccaacagatt gcagatttaa tcggtaagaa aatcgatgaa
    18661 atcttcttta cctctggtgg aacagaaggg gataactggg ttatcaaggg tgtggccttt
    18721 gaaaaagctc agtttggcaa gcacatcatt gtttcagcca ttgaacatcc agcagtcaaa
    18781 gagtcagccc tctggttgaa aagtcaagga tttgaagtgg attttgctcc agttgataag
    18841 aaaggcttgg tcgatgttga ggcgttagca ggtttgatac ggcctgatac aatcctcgtt
    18901 tccatcatgg ctgtgaacaa tgaaatcggc tctatccaac ctattgaggc tatttcagaa
    18961 ttcttggcag acaagccgac tatttccttc cacgttgatg cggttcaggc gcttgccaaa
    19021 attccgactg aaaagtatct gacagaacgg gtggattgcg cgaccttctc gagtcataaa
    19081 tttcatggtg tccgtggtgt tggctttgtc tatatcaaat ctggcaagaa gattacacct
    19141 cttcttacag gtggtggcca ggagcgagat tatcgttcga caactgaaaa tgtggcaggg
    19201 attgcagcga cagccaaggc tctccgttta tctatggaaa agctagatat ctttaggagc
    19261 aagactgggc agatgaaggc agtgattcac caagctcttc tgaactatcc ggatattttt
    19321 gtcttttcag atgaggaaga ctttgcacct catattctga cttttggaat caaaggtgtt
    19381 cgaggtgaag tcatcgttca cgcctttgaa gactatgata ttttcatctc aacaacctca
    19441 gcttgttcat ctaaggcagg aaaaccagcc ggtaccttga ttgccatggg agtggacaaa
    19501 gataaggcca agtcagctgt gcgtcttagc ctagacttgg aaaatgatat gagtcaggtc
    19561 gagcagtttt tgaccaagtt aaaattgatt tacaatcaaa ctagaaaagt aagataggag
    19621 cattcatgca gtattcagaa attatgattc gctacggaga gttgtcaacc aagggtaaaa
    19681 accgtatgcg tttcatcaat aaacttcgta ataatatttc ggacgttttg tctatctata
    19741 cccaagttaa ggtaacagca gatcgcgacc gtgcccacgc ttacctcaat ggagctgatt
    19801 acacagcagt tgcagaatct ctcaaacaag tttttggaat tcaaaacttt tctcctgttt
    19861 ataaggttga aaaatctgta gaagttttga agtcttctgt ccaagagatt atgcgggaca
    19921 tctacaagga aggtatgacc tttaagattt ctagcaagcg tagcgaccac aactttgaac
    19981 ttgatagtcg tgaactcaac caaacacttg gaggggctgt attcgaagcc attccaaatg
    20041 tgcaagttca aatgaaaagt cctgacatca atcttcaggt ggagattcgt gaagaagcag
    20101 cctatctttc ttatgaaacc attcgtgggg ctggtggttt gccagttgga acttcaggta
    20161 aagggatgct catgttgtca ggagggattg actcacctgt agcaggttat cttgctctta
    20221 agcgtggggt ggatatcgag gcagttcact ttgctagtcc accatatact agtcctggtg
    20281 ccctcaagaa agcgcaggac ttgacccgta aattgaccaa gtttggcgga aatatccagt
    20341 ttatagaggt gcctttcaca gagattcaag aggaaatcaa agccaaagcg ccagaagctt
    20401 atttgatgac tctaactcgt cgctttatga tgcggattac tgaccgtatt cgtgaggtac
    20461 gaaatggttt ggttatcatc aatggggaaa gtctaggtca agtagccagc caaacccttg
    20521 aaagtatgaa ggctatcaat gctgttacca acactcccat cattcgtcct gtggttacca
    20581 tggacaagtt ggaaatcatt gacatcgccc aggaaatcga tacctttgac atttcaatcc
    20641 aaccgtttga agactgttgt accatttttg caccagatcg tccaaaaaca aatcctaaaa
    20701 ttaagaatgc ggagcagtac gaagcgcgta tggatgttga aggcttggtt gagcgagcag
    20761 tggctggaat catgattact gaaatcacac ctcaagccga aaaagatgaa gttgatgact
    20821 tgattgataa tctactctaa ttcagaaaat ccaaaagaat agcgaaaatc agtaaaaaaa
    20881 gttagttttt tctctaaaaa caggtaaaaa actaactttt
//
LOCUS       DBERDK010000033        20671 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.033, whole genome shotgun sequence.
ACCESSION   DBERDK010000033 DBERDK010000000
VERSION     DBERDK010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 20671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20671
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.033"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(128..1264)
                     /gene="lctO"
                     /locus_tag="ACL73L_001189"
     CDS             complement(128..1264)
                     /gene="lctO"
                     /locus_tag="ACL73L_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HGS0772173.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TLRENIRAFNHKLIVPHTLCDVENPSTEIEFAGEKLSSPIIMAPVAAHKLANEQGEVA
                     TARGVHEFGSLYTTSSYSTVDLPEISEALQGTPHWFQFYFSKDDGINRHIMDRVKDEG
                     YKAIVLTADATVGGNREVDKRNGFVFPVGMPIVEEYLPEGAGKSMDFVYKSAKQRLSP
                     RDVEFIAEYSGLPVYVKGPQCREDVERSLAAGASGIWVTNHGGRQIDGGPAAFDSLQE
                     VAEAVDRRVPIVFDSGVRRGQHVFKALASGADLVAIGRPVIYGLALGGSVGVRQVFEH
                     LNAELKTVMQLSGAQTIEDVKHFKLRHNPYNPTFPVDPRDLKLY"
     gene            complement(1888..3378)
                     /gene="lysS"
                     /locus_tag="ACL73L_001190"
     CDS             complement(1888..3378)
                     /gene="lysS"
                     /locus_tag="ACL73L_001190"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HGS0772174.1"
                     /translation="MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQEL
                     KDKYANLDKEQLHDKNETATIAGRLITKRGKGKVGFAHLQDREGQIQIYVRKDAVGEE
                     NYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
                     YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINEPFKRVHMVDAIREITGVD
                     FWQDMTLEEAKAIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVS
                     PLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATG
                     IDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            complement(3518..3652)
                     /locus_tag="ACL73L_001191"
                     /pseudo
     CDS             complement(3518..3652)
                     /locus_tag="ACL73L_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            3868..4548
                     /locus_tag="ACL73L_001192"
     CDS             3868..4548
                     /locus_tag="ACL73L_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGS0772175.1"
                     /translation="MDWSIVEQYLPLYQKAFFLTLHIAVWGILGSFLLGLIVSIIRHY
                     RIPVLAQVATAYIELSRNTPLLIQLFFLYFGLPRIGIVLSSEVCATLGLVFLGGSYMA
                     ESFRSGLETISQTQQEIGLAIGLTPLQVFRYVVLPQATAVALPSFSANVIFLIKETSV
                     FSAVALADLMYVAKDLIGLYYETDIALAMLVVAYLIMLLPISLVFSWIERRTRHAGFG
                     NPSTLSRK"
     gene            4508..5185
                     /locus_tag="ACL73L_001193"
                     /pseudo
     CDS             4508..5185
                     /locus_tag="ACL73L_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154125.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            5186..5943
                     /locus_tag="ACL73L_001194"
                     /pseudo
     CDS             5186..5943
                     /locus_tag="ACL73L_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000401.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            5955..6197
                     /locus_tag="ACL73L_001195"
     CDS             5955..6197
                     /locus_tag="ACL73L_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HGS0772176.1"
                     /translation="MKLFKPLLTVLALAFALIFITACSSGGNAGSSSGKTTAKARTID
                     EIKKSGELRIAVFGDKKPFGYVDNDGSYQGYATILN"
     gene            6239..6820
                     /locus_tag="ACL73L_001196"
     CDS             6239..6820
                     /locus_tag="ACL73L_001196"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HGS0772177.1"
                     /translation="MSVDAANRAEYLISNKVDITLANFTVTDERKKQVDFALPYMKVS
                     LGVVSPKTGLITDVKQLEGKTLIVTKGTTAETYFEKNHPEIKLQKYDQYSDSYQALLD
                     GRGDAFSTDNTEVLAWALENKGFEVGITSLGDPDTIAAAVQKDNQELLDFINKDIEKL
                     GKENFFHKAYEKTLHPTYGDAAKADDLVVEGGH"
     gene            complement(6900..7547)
                     /locus_tag="ACL73L_001197"
     CDS             complement(6900..7547)
                     /locus_tag="ACL73L_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0772178.1"
                     /translation="MNEIIILKNIELKLKKTCVFQNLNFSCKQGEIIGITGANGSGKS
                     VLFKLIAGLYSPSYGEVLINGENIVPERKIPANLGALIEEPGFINYYSGFKNLQYLAS
                     IRGVVGNQEINDTLKIVGLYEQKDQKVKTYSLGMRKKLGIAQAIMENPSILLLDEPMN
                     ALDKSSVENMRTLFRKLSSEKGTTILIASHSEEDIRILCDKVYAIEDKVCTLCSD"
     gene            complement(7544..8242)
                     /locus_tag="ACL73L_001198"
     CDS             complement(7544..8242)
                     /locus_tag="ACL73L_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772179.1"
                     /translation="MGKLSSHMWRLNQIIYTKYFWGYVLFWILICLGLWYWLEGNDRL
                     VIEILKGPNLSQNSFLVLSIWLLHWFIIHTFFLAVVYRRRASDFFMEVIRFSSIKLWI
                     RYQIWTCFLYGLILIMVKVLVIQFMLQLPNWDIGVLFIVDSLNACVLVLFCFMLYALG
                     ANVQMNFACVSFFLLMIVFGGLFVGNRTNYLFYILNRGNGDIGRDLFLQLLFLVFLFQ
                     SIFYFTRQKRRFIE"
     gene            complement(8252..9003)
                     /locus_tag="ACL73L_001199"
                     /pseudo
     CDS             complement(8252..9003)
                     /locus_tag="ACL73L_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9003..9767)
                     /locus_tag="ACL73L_001200"
     CDS             complement(9003..9767)
                     /locus_tag="ACL73L_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772180.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESHFLTAQLCRISKKKYLAIVG
                     STVIISSFLVFFLPYLLLLGINLLVTPYQEIYIGDYSGALKELFDSNQFLYSLVTTLW
                     YGVWGTVFSIFGLASALLVKKKIGAIFIPVAYMMVGGIFWAILGLSYLEPVTTLALGY
                     QKDISLSLVSGHLAFILFVSCLVVYGTFFLHSEDYV"
     gene            complement(9863..9985)
                     /locus_tag="ACL73L_001201"
     CDS             complement(9863..9985)
                     /locus_tag="ACL73L_001201"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772181.1"
                     /translation="MYYNGGGDNYIGSGTRLAMAPQFGNHVRIHTSGSWNPDSY"
     gene            complement(10446..11078)
                     /gene="pyrE"
                     /locus_tag="ACL73L_001202"
     CDS             complement(10446..11078)
                     /gene="pyrE"
                     /locus_tag="ACL73L_001202"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HGS0772182.1"
                     /translation="MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPETRTLIENGFVEAIKEAFPEVEVIAGTATAGIPHGAIIADKMDLPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
                     KNFTDAGVKLVTLSNYSELIHLAQEEGYITPEGLDLLKRFKEDQENWQEG"
     gene            complement(11112..11813)
                     /gene="pyrF"
                     /locus_tag="ACL73L_001203"
     CDS             complement(11112..11813)
                     /gene="pyrF"
                     /locus_tag="ACL73L_001203"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HGS0772183.1"
                     /translation="MREHRSIIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKILSQLGVDMTNVHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
                     QVIKQATNPDFICLTPGIRPAGVAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPV
                     AAYHAIKDEWTQDWN"
     gene            complement(12046..13245)
                     /gene="divIB"
                     /locus_tag="ACL73L_001204"
     CDS             complement(12046..13245)
                     /gene="divIB"
                     /locus_tag="ACL73L_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HGS0772184.1"
                     /translation="MSKDKKNEDKETLEELKELSEWQKRNQEYLKKKAEEEAALAEEK
                     EKERQARMGEESEKSEDKQDQESETDQEDSESAKEESEEKVASSEADKEKEEIEESES
                     KEKEEQDKKLAKKAIKEKPAKAKIPGIHILRAFTILFPSLLLLIVSAYLLSPYATMKD
                     IRVEGTVQTTADDIRQASGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQFPTK
                     FTIKVKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYLSVLFNDSEQIKVFV
                     SELAQISPELKAAIQKVELAPSKVTSDLIRLTMNDSDEVLVPLSEMSKKLPYYSKIKP
                     QLSEPSVIDMEAGIYSYTVADKLIMEAEEKAKQEAKEAEKKQEEEQKKQEEESNRNQT
                     TQRSSRR"
     gene            complement(13255..14313)
                     /locus_tag="ACL73L_001205"
     CDS             complement(13255..14313)
                     /locus_tag="ACL73L_001205"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HGS0772185.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGDKRGIEHQ
                     EILKSGLDVTFHSIATGKLRRYFSWQNMLDVFKVGWGIVQSLFIMLRLRPQTLFSKGG
                     FVSVPPVIAARVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLAKVEHVGA
                     VTKVSDKNTPEPDELVDIQTHFNPKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELRQNLFRVDYVTDLYQPLMELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQIENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            complement(14317..15669)
                     /gene="murD"
                     /locus_tag="ACL73L_001206"
     CDS             complement(14317..15669)
                     /gene="murD"
                     /locus_tag="ACL73L_001206"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HGS0772186.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     DNPAAQSLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYNNPMIEKALAKGIPVLTE
                     VELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIASD
                     KDTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGSFSEYVAAKWNIQNKMTAAD
                     FLVLNFNQDLAKDLTSKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPG
                     SHNVENALATIAVAKLRDVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNI
                     LATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKA
                     GVAYVEATDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
                     "
     gene            complement(15773..16414)
                     /locus_tag="ACL73L_001207"
     CDS             complement(15773..16414)
                     /locus_tag="ACL73L_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0772187.1"
                     /translation="MIELKNISKKFGSRQLFSDTNLHFEGGKIYALIGTSGCGKTTLL
                     NMIGRLEPYDKGQIIYDGTSLKDIKPSVFFRDYLGYLFQDFGLIESQTVKENLNLGLV
                     GKKLKEKEKISLMKQALNRVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILAD
                     EPTASLDPKNSEELLSILESLKNPNRTIIIATHNPLIWEQVDQVIRVTDLSHR"
     gene            complement(16416..17834)
                     /locus_tag="ACL73L_001208"
                     /pseudo
     CDS             complement(16416..17834)
                     /locus_tag="ACL73L_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
     gene            18001..19347
                     /locus_tag="ACL73L_001209"
     CDS             18001..19347
                     /locus_tag="ACL73L_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISSpn5 family transposase"
                     /protein_id="HGS0772188.1"
                     /translation="MNSLPNHHFQNKSFYQLSFDGGHLTQYGGLIFFQELFSQLKLKE
                     RISKYLVTNDQRRYCRYSDSDILVQFLFQLLTGYGTDYACKELSADVYFPKLLEGGQL
                     ASQPTLSRFLSRTDEETVHSLRCLNLELVEFFLQFHQLNQLIVDIDSTHFTTYGKQEG
                     VAYNAHYRAHGYHPLYAFEGKTGYCFNAQLRPGNRYCSEEADSFITPVLERFNQLLFR
                     MDSGFATPKLYDLIEKTGQYYLIKLKKNTVLSRLGDLSLPCPQDENLTILPHSAYSET
                     LYQAGSWSHKRRVCQFSERKEGNLFYDVISLVTNMTSGTSQDQFQLYRGRGQAENFIK
                     EMKEGFFGDKTDSSTLIKNEVRMMMSCIAYNLYLFFKHLAGGDFQTLTIKRFRHLFLH
                     VVGKCVRTGRKQLLKLSSLYAYSELFSALYSRIRKVNLNLPVPYEPPGRKASLMMH"
     gene            complement(19537..20145)
                     /locus_tag="ACL73L_001210"
                     /pseudo
     CDS             complement(19537..20145)
                     /locus_tag="ACL73L_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
     gene            complement(20216..20515)
                     /locus_tag="ACL73L_001211"
                     /pseudo
     CDS             complement(20216..20515)
                     /locus_tag="ACL73L_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
BASE COUNT         6292 a         4449 c         3700 g         6230 t
ORIGIN      
        1 aaatcgattt aactgtcctg attaatttgt cctattctta tttcatttta ttatagtttt
       61 cgatagcgat ttattcttcc aatacacgaa gaaaaacctc cacattcagt ggaggcaatc
      121 tgttttatca atacaatttt aagtcacgag ggtcaactgg gaaggttggg ttgtatggat
      181 tgtgacggag cttaaagtgt ttgacatctt caatggtctg agctccagac aattgcataa
      241 ctgtcttcaa ttccgcattc aagtgttcaa agacttgacg cacaccgaca ctaccaccga
      301 gagccaagcc ataaataaca gggcgaccaa tagctaccaa gtctgctcct gaagccaagg
      361 ctttaaagac gtgttgacca cgacgaacac cagagtcaaa gacaatcggc acacgtctat
      421 caactgcttc tgccacttct tgaagcgagt caaaggcagc tggtccaccg tcgatttgac
      481 gaccaccgtg gttggttacc cagataccag aagctcctgc agcaagcgaa cgttcaacgt
      541 cctcacggca ttgtggtccc ttgacataca caggaagacc agagtattca gcgataaatt
      601 ctacatcgcg tggagacaag cgttgtttag ctgatttgta aacaaagtcc attgatttac
      661 cagcaccttc tggcaggtat tcttcaacaa tcggcatgcc aactgggaag acaaaaccat
      721 tacgcttatc cacttcacga ttccccccta cagtagcatc tgccgtcaag acaatcgctt
      781 tataaccttc atccttcaca cggtccatga tgtggcggtt gataccgtca tccttactaa
      841 agtaaaattg aaaccaatga ggtgtccctt gaagggcttc agaaatctct ggaaggtcaa
      901 cagtagagta agaactggtt gtataaagag aaccaaactc atgcacacca cgcgcagtcg
      961 ccacttcccc ctgttcattt gccaatttat gagccgcaac aggtgccata atgattggcg
     1021 aagatagttt ttcacctgca aattcaatct ctgtacttgg attttcaaca tcgcaaagtg
     1081 tatgaggaac gatgagtttg tggttaaagg cgcggatatt ctctcttaaa gtgaaagtat
     1141 cttccgcccc actagcgata tagccaaatg ctgctttagg aataacttgt tgcgccattg
     1201 gctccaaatc ataggtattg atgaagtcta catgaccttc tgcattgctt gttttgtatg
     1261 acataaactg tcctcctcga ttaagtaagc gtttgctttt tactatacaa ctataataac
     1321 tcttttttca atcattttca agagttttgc ttaaaaacca tcttattttt tcatacaata
     1381 tactagccct actttcctta aactctaaac cttagtctat attaagtgaa tcgttgagat
     1441 tttttgctgc caggcatttg aactactttt ttatttgctg attctatcta ctaatactat
     1501 aaaaaacaag tgaataagac accagaacat cctctaatag gacctatgga cttatatcag
     1561 tcccatatat actgtttaag caagatacta tcctagttga cgctaacttc ctatcccact
     1621 tattaatttt ttatttatag tgaatctgaa caaatcaatt ggggaatcca agtcaatttc
     1681 tagtactttt tgaaaagttt cagcgtacta tcgtaaaatg aaataaaaca tgcacaaatt
     1741 gattagggaa tttaaaccaa tttctaacaa tgtcttagaa atcaaagtgt actatttcaa
     1801 tttcaataca ctacacttca ttttctcctt aaattcagtc tctttttgaa taaaaaaatc
     1861 ctctggtaag agccagagga taagaattta tttcattgtt gggaagagca atacatcacg
     1921 gatagttgtt gtatcagtga ggagcatgca gagacggtcg ataccgattc ccaaaccacc
     1981 tgttggtggc ataccgtatt caagagcttc aatgtagtca tagtcgattc ctgtcgcttc
     2041 atcatcacca agttctttgg ctttagcttg ggcttcaaaa cggctaagtt ggtcgattgg
     2101 gtcgttgagc tcagtaaagg cattaccgta ctccttagtc atgataaaga gctcgaaacg
     2161 gtcagtaaag cgttggtctt caggattttt cttagcgagt ggagatacag ctactggatg
     2221 tccatagaca aaggttggtt ggattaaagt ttcttcaaca aactcttcaa agaaggcatt
     2281 gatgatgtga ccaacctcag tgtagtgttt ctcaactgga actttcttct cagcagcgat
     2341 agctttagct tcttccaaag tcatgtcttg ccagaaatcg acaccagtaa tttctctgat
     2401 agcatccacc atatgaacac gcttaaatgg ttcgttaatc ttgatttcag taccttgata
     2461 gttgactggg ccatcgccct taactgattt agcagcatgt tggataatgc cttccgtcaa
     2521 gtccatgata tcttggaagt ctgcataagc ttggtaaact tcgatagaag tgaactcagg
     2581 gttatgagta gcatccattc cttcgttacg gaagatacgc ccaatttcat agacacgttc
     2641 cataccaccc acgataaggc gttttaagtg aagctcagtc gcgatacgaa gcaccatgtc
     2701 aatgttttgg gcattgtggt gggtgataaa tggacgggca gcagcaccac cagcttcgtt
     2761 gtgaagaaca ggtgtttcca cttcaaggaa tcctttttgg tcaaggtaac gacggatttc
     2821 agagatgatt tttgaacgag tgacaaaacg ttcaaagctt tcacgattag aaatcaagtc
     2881 aaggtaacgt ttacggtaaa ttgtttcaac gtctgtcaaa ccatggaatt tctcaggaag
     2941 aggacgaaga gccttagaca agtgtgtgat gtgggttgcc ttgatagaga gttctcccat
     3001 atccgtacgc atcacttcac cttcgacacc aaggaagtca ccaagatctg cttttttgaa
     3061 gatttcgtag ttttcttcac cgacagcatc cttacgaacg tagatctgaa tctggccttc
     3121 gcggtcttga aggtgggcaa aaccaacttt tcctttacca cgtttggtta tcaagcgtcc
     3181 tgcgatagta gctgtttcgt ttttatcgtg taattgttct ttatcgaggt tggcatattt
     3241 atcttttaat tcttgtgaat ttgcagtacg ttcaaaacgt tttccgaaag gatcgattcc
     3301 ttgttcgcgg agcgcagcca ttttttcacg gcgaacgatc tgctggtcat ttagttcttc
     3361 catatgttct gtagacatgg gatcctccta gttttactca aaattgaata cgatgagtca
     3421 ttttttgcga tactcccatt ttatcataaa taagcaagaa attcacggat aatctttaaa
     3481 aactaaaaag aacctgacgc taaaatcact ggaaagaagt gaaagtatct cccgccccac
     3541 tagcgatata gccaaatgct gctttaggaa taacttgttg cgccattggc tccaaatcat
     3601 aggtattgat gaaatctaca tgaccttctg cattgcttgt tttgtatgac ataaaatgtc
     3661 ctccttaata agtaagcgtt tactttgtat attataaaaa tatcttaact cttttttcaa
     3721 aacttttcaa atattttgtt tggaaatttc agaaatttta tgtctatggt aaaaaatcct
     3781 tataacggca ataaaaaata gatattatcc aaagaagatt ttaagtgcta caataactgt
     3841 attatttcta gatgggaggt tctatttttg gattggtcca ttgttgaaca atatctacca
     3901 ctatatcaaa aggcattctt tctgaccttg catattgcag tttggggaat tttgggatcc
     3961 tttctgctcg gtttaatcgt tagtatcatc cgacattatc gaatccctgt tttggcgcaa
     4021 gtagcgacag cctacattga attgtcacgt aatacgcccc ttttgattca actcttcttt
     4081 ctctacttcg gtcttccccg aatcggcatt gtcctatctt cagaagtctg tgcaacgctt
     4141 gggcttgtct ttttaggagg ctcctatatg gcagaatctt tccgaagtgg gctggaaacc
     4201 atcagtcaaa cccagcagga gattggcctc gctattggtc tgacacctct acaggtcttt
     4261 cgctatgtgg ttcttccgca agcaacagcg gtggcactcc cctcctttag tgccaatgtc
     4321 attttcctta tcaaggaaac ctctgttttc tcagcagtgg ctttggccga cctcatgtac
     4381 gtcgccaagg atttgattgg tctctactat gagacagaca ttgcgctagc tatgttggta
     4441 gttgcttatc tgatcatgct gctacccatc tcactggtct ttagctggat agaaagaagg
     4501 acccgccatg caggattcgg gaatccaagt actctttcaa ggaaataatc tcctgagaat
     4561 cttacagggg ttgggcgtta cgattgggat atccatcctg tctgtcctct tatccatgat
     4621 gttcggaaca gtcatgggaa tcatcatgac ctcccattct agaatcatac gatttttaac
     4681 acgattgtat ctggacttta tccgtatcat gccccagctg gtgctactct tcatcgttta
     4741 ctttggcttg gctcgaaact ttaatatcaa tatctcaggt gagacttcag ctattatcat
     4801 ttttaccctc tggggaacag ctgaaatggg agacttggta cgtggagcta tcacttctct
     4861 ccctaaacat cagtttgaaa gtggataggc actcggcttg actaatgttc aactttacta
     4921 ccacatcatc atcccacaag tcttaagaag actgctaccg caggctatca atcttgtcac
     4981 tcggatgatt aaaaccactt cattagttgt tttgattggg gttgtggaag tgaccaaagt
     5041 tggacaacaa atcatcgata gcaatcgcct gaccatccca actgcttcat tttggattta
     5101 tggaaccatt ctagtcttat atttcgcagt ttgctaccct atttccaaac tatccactca
     5161 cttagaaaaa cattggagaa actaaatgtc tgaaactatc ttagaaatca aggaactaaa
     5221 aaaatccttc ggagacaatc ccatcctcca aggactttct ctagaaatca aaaaagggaa
     5281 gttgttgtca tcctagggcc atctggttgt gggaaaagta ccctccttcg ttgcctcaac
     5341 ggcttagaaa gtattcaagg tggagatatt cttctggatg gtcagtctat cgttgaaaat
     5401 aaaaaagatt ttcacctagt tcgccaaaag attggcatgg tctttcaaag ttatgaactc
     5461 tttccccatc tggatgtctt acaaaacctc atcctaggcc ctatcaaagc tcaaggaagg
     5521 gacaagaaag aagtaacgga agaagctttg caattactag agcgtgtcgg tttgctggat
     5581 aaacaacata gctttgcccg tcaattatct ggtggacaga agcaacgtgt tgcaattgtc
     5641 cgtgccctcc taatgcatcc agaaatcatc ctttttgacg aggtgactgc ttcgctggat
     5701 ccagaaatgg tgcgtgaggt gctggaactt atcaatgatt tggcccaaga aggccgtacc
     5761 atgattttag taacccacga aatgcagttt gcccaagcca ttactgaccg gattatcttc
     5821 ctcgaccaag ggaaaatcgc tgaagaagga acagctcaag ccttctttac caatctgcaa
     5881 accaaacgag cccaggaatt tttaaacgtc tttgacttta gccaattcgg ctcatatcta
     5941 taaaggagat tcttatgaaa ctattcaaac cactcttaac tgttttagca cttgcctttg
     6001 cccttatctt tatcactgct tgtagctcag gtggaaacgc tggttcatcc tctggaaaaa
     6061 caactgccaa agctcgcact atcgatgaaa tcaaaaaaag cggtgaactg cgaatcgccg
     6121 tgtttggaga taaaaaaccg tttggctacg ttgacaatga tggttcttac caaggctacg
     6181 ctacgatatt gaactaggga accaactagc tcaagacctt ggtgtcaagg ttaaatacat
     6241 ttcagtcgat gccgccaacc gtgcggaata cttgatttca aacaaggtag atattactct
     6301 tgctaacttt acagtaactg acgaacgtaa gaaacaagtt gattttgccc ttccatatat
     6361 gaaagtttct ctgggtgtcg tatcacctaa gactggtctc attacagacg tcaaacaact
     6421 tgaaggtaaa accttaattg tcacaaaagg aacgactgct gagacttatt ttgaaaagaa
     6481 tcatccagaa atcaaactcc aaaaatacga ccaatacagt gactcttacc aagctcttct
     6541 tgacggacgt ggagatgcct tttcaactga caatacggaa gttctagctt gggcgcttga
     6601 aaataaagga tttgaagtag gaattacttc cctcggtgat cccgatacca ttgcggcagc
     6661 agttcaaaaa gacaaccaag aattgctaga cttcatcaat aaagatattg aaaaattagg
     6721 caaggaaaac ttcttccaca aggcctatga aaaaacactt cacccaacct acggtgacgc
     6781 tgctaaagca gatgacctgg ttgttgaagg tggccattaa ctcttaaaag ggactggatt
     6841 ttaagctcca atcccttttt aagattttac ctataacatc ctgagtctat ctaagatgtt
     6901 caatctgaac acagtgtaca tactttatct tctattgcat atactttatc acataagata
     6961 cgaatatcct cttcactatg actagcaatc aaaattgttg tccctttttc actagagagc
     7021 tttctaaaca atgttctcat attttctaca cttgatttat ccaaggcatt cataggttca
     7081 tctagtaaaa gaatagaggg attctccata attgcttgag caatccctag ctttttcctc
     7141 atacctagcg aataagtttt aactttctgg tctttttgct catatagacc aactattttc
     7201 agtgtatcat tgatttcctg attaccaact actcctcgta tgcttgccaa atattgtaaa
     7261 ttcttaaagc cactataata atttataaaa ccaggttctt caatcaaagc tcccaaatta
     7321 gctggaattt ttctctcagg aacaatattt tccccattga ttaacacttc tccataagac
     7381 ggactatata aaccagctat taatttaaac aatacacttt tccctgagcc attcgcacca
     7441 gtaattccta taatttcccc ctgtttacaa ctaaagttaa ggttttgaaa aacacatgtc
     7501 ttttttaatt tcaactcaat attttttaat atgattattt cattcattct ataaacctcc
     7561 tcttttgacg agtgaaatag aaaatgcttt gaaaaagaaa gactaaaaat agcaactgaa
     7621 gaaataaatc tcgtcctata tctccattcc ctcgattcaa aatataaaat agataattag
     7681 ttcgatttcc tacaaataga ccaccaaaca caatcatgag taaaaagaaa ctaacgcaag
     7741 caaagttcat ttgtacattc gctcctagtg cgtataacat aaagcaaaac aagactaaca
     7801 cacaagcatt caaagaatca actataaaca aaactcctat atcccagttt ggtaactgta
     7861 acataaattg aatcactaga acttttacca tgattaaaat gagtccataa agaaaacagg
     7921 tccaaatctg atacctaatc cagagcttaa tagaagaaaa tcgaatcact tccataaaga
     7981 aatcggatgc tcttctacga tatacaactg ctagaaaaaa tgtatgaata ataaaccaat
     8041 gaagcaacca tatagataag actaaaaaag agttttgact cagattaggc ccttttaaaa
     8101 tttctataac aagtctatca tttccttcta accaatacca taatcctaaa caaatcaata
     8161 tccaaaaaag aacataaccc caaaagtact tggtatagat tatctgattc aacctccaca
     8221 tgtgactact taactttccc atttccaact tttacactct cctccagttt ctatgaacag
     8281 aaaatacagt caatggaatt atgatagata gtaatgtaag ccaataaatc aatgcatacg
     8341 gattggaatt taccatatac tgaaaaattg gcaataatcc aattatccca ttgtccaact
     8401 gataaatcca gaataaggat tctacaaata aaaaagtaaa cagaaatact aagctcatgt
     8461 ttgatttttt aaaccaagaa aaactgctaa tagtaagtaa ggataaaaag aaatagtatg
     8521 ctatataaga gaaaaaaaat cctataaaga aactagcatt gtttgcaata cttataccat
     8581 aaaattctct taaaaaatca acctccttta tctccaaaga gaagctaaaa ccattactaa
     8641 atgcaattag aaaaatcaat aaaaataaag tcgccgtcca aatccccgta ctaagagctg
     8701 ctaatttgaa actaaaactg gtaaagtgct taattgattt cagacgaatg cgacactcca
     8761 acctattaaa atagttattc atcaaataaa aaaagaacaa tatatatgtg aacggaaagc
     8821 aatatactcc agtcgtcata tcttgaagta aaactaagat ccattctaat acatttggat
     8881 ggattgaata ttggcgacag cgcaataaat atactgtact agataaaaca caggatagca
     8941 gtaatataaa taaaccaata ctgataaaaa atcttttttg taaaattgaa caaattgttt
     9001 cattatacgt agtcctctga atgtagaaaa aatgtaccat aaacaaccaa acaactaaca
     9061 aataaaataa aagcaagatg cccactaact aaggaaagac tgatatcttt ctgatatccc
     9121 aaagctaatg ttgtcacagg ttctaagtaa gatagcccta aaatagccca aaaaatacca
     9181 ccaaccatca tataggcaac tgggatgaaa atagctccta tttttttctt cactagcaaa
     9241 gcactagcta gtccaaaaat agagaacaca gtgccccaaa ctccatacca gagagtcgtt
     9301 acaagactat agagaaactg attggaatca aataattctt ttaaggcacc actataatct
     9361 ccaatataaa tttcctgata aggagtcact aaaagattaa ttcctaataa taataaatag
     9421 gggagaaaaa agactagaaa agaagaaata attacagtac tacccacaat agccagatac
     9481 ttctttttag aaattcgaca caattgtgct gttagaaaat gactctcagc atcctctatt
     9541 atctgactag aatagggcag tgtacagata agtgcagcta ctaggctaat cggtgaaaat
     9601 cctcgaatag aagaagcggc aaaaaaaatc gataaacctt caattttata aataccattg
     9661 aaagcaagga aatttcctaa actcatgcaa agaagggtca aaaataaaga catataaaat
     9721 cgaggtgatt gaacgactcg tacaagatta caaatgaaaa attccatcct tactcctcct
     9781 tataataaaa atagggtgta gcattctttt ttcatgctac acccacaatc aaccatctct
     9841 aaggcttact ctgacaagta agttaataag aatctggatt ccaagaacct gaagtatgaa
     9901 ttcttacatg atttccaaat tgtggcgcca tagctaatct agtaccagaa ccaatataat
     9961 tgtcaccacc tccattatag tacatgacaa tcctagagcc agaccccaat gaatataccg
    10021 gggtaatatc tgacccacta taggcgctac gaatagaggt atttaacctt ttaccgccac
    10081 cagtgctgtc attattatta attccagcag aggcgttttc tttctcaagc tcactagttg
    10141 tatccgaacc ccaacgcggc gacgttaaat cataatctcc aatctgtcta gctaaaactg
    10201 tagggagaaa caaactagat gcaattgtaa ctacgcaaaa aaggaatata tttttcttct
    10261 tttcatgaca tcgtcctctt aagaagtgat aaacaagata gttgattgat caatttgata
    10321 catattacat tttcattata tcaataaaag aaagcgttgt caattatttt atttaaaata
    10381 ttgtcatatt ttaaataaaa tggtaaaaga gactcggtag cctcgtctct ctttatctta
    10441 ctgacctaac cttcttgcca attttcttgg tcttctttaa agcgttttag aagatcaagg
    10501 ccctctggcg tgatgtaacc ttcttcttgg gctagatgga taagctcgct atagtttgaa
    10561 agcgtcacaa gtttaacacc agcatctgta aagttcttat ctgcttttgg caattggtag
    10621 ctgaaaatcg ctacaactcc aagtacatct gctccttctc gcttggctgc tgctacagct
    10681 tcaagaactg aaccacccgt tgaaataagg tcttcaacca ctaccatttt ttgaccttga
    10741 gctacgcgac cttcgatttg attaccagct ccgtggtctt ttggtttgct acggatgtag
    10801 gcaaaaggca agtccatctt atcagcaata atggctccgt gtggaatccc tgctgttgca
    10861 gttcctgcaa tcacttctac ttcaggaaag gcttctttga tagcttccac aaaaccattt
    10921 tcaattaggg tacgagtttc tggataggct agtgtcacac gattatcagt gtaaatcggt
    10981 gacttgatac cagatgccca agtgaagggt tcctctggtt tgaggtaaac ggcttggatt
    11041 ttcaagaggt ggctagcgat atctttagca agtgtcatgg tattctcctt ttatttttct
    11101 aatctagttc tttaattcca gtcctgtgtc cattcatcct tgatggcatg ataagctgca
    11161 acaggatcct cagcttgggt aatgggacgt cctactacga tatagtcact gccgatttga
    11221 taggcatcag caggtgtcat gactcgtttt tgatctccaa ctgcaacacc agctggacga
    11281 atccctggtg tcagacagat aaaatctgga ttggtagcct gcttgatgac ttgtacttcc
    11341 tgagccgagc aaacaacacc atccaagcca gcttcagctg tcttcttggc atagtgaatc
    11401 acagactctt gcagactggt ttggatattt tgaaactcct gcatctgagc ttctgacgtt
    11461 gatgtgagct gagttacagc gatcaattta gcttgacttc caagaccttc acgcgcagcc
    11521 ttcatcatct ctacaccacc agccgcatga acattggtca tatccacacc aagctgagat
    11581 aggatcttca tggctgactt gactgtatta ggaatgtcat gaagtttgag atccaaaaag
    11641 acactatgac ccaaaccttt taagtaggac acaatctcag gccccgctgc gtaataaagc
    11701 tccatcccta ccttgagata aaggctttct tctgctggga aaagagctaa aaattccttg
    11761 accgcctcaa aactaggaaa atcaagagca atgattgaac gatgttctcg cataggtttc
    11821 tccttttacc tttactagtg agagagtctg gccaacagaa gccacgaaaa aaggaacctg
    11881 ccaacagcaa ggttccacga aaaatgggta gtctccaccc tttcaccgtc taaccttagc
    11941 tgcctctctg gactgcttta aaggttttct attattctat tatttctact agcacttgtc
    12001 aagtaaaaac atggtcacta aagaactata agagaaaagg taaacctagc gacgcgatga
    12061 acgctgggtc gtttggtttc gattgctctc ttcctcttgt tttttctgtt cttcttcttg
    12121 ttttttctca gcttccttgg cctcttgttt ggctttttcc tcagcttcca taattaattt
    12181 atccgccaca gtgtaactgt aaattccagc ttccatgtca atcacactcg gttctgacaa
    12241 ttgtggctta atcttactgt aatatggcag tttcttactc atttcagata gaggaaccaa
    12301 gacttcgtcc gaatcattca tggtcaatcg aattaaatcg gatgtcacct tgcttggggc
    12361 taattccacc ttttggatag ccgccttgag ttctgggcta atttgagcaa gttctgagac
    12421 aaaaaccttg atttgttcac tatcattaaa gagaactgat aaataagttt ctggtaaact
    12481 gttcagactc acagaactag tctcaagctg accactggaa agaataggat aatgattttc
    12541 accagaaata tagtaggcca caatatcata ttccttgacc ttaatagtga acttagttgg
    12601 aaattgatag acaagttgag ctgattcaac ccaatagtta gacttaatct gcttttcata
    12661 ttttgccttg tctagcagaa ggttaatcgt ataatccgaa tcctgaatgc ctgaagcctg
    12721 tcgaatatca tcagctgtag tttgcaccgt tccctcaaca cgaatatctt tcatggtcgc
    12781 ataaggactg agcaagtagg cagagacaat caataaaagc agacttggaa ataaaatcgt
    12841 gaaggctcgc aagatatgga taccaggaat ctttgctttg gctggttttt cctttatagc
    12901 ctttttagca agctttttat cctgttcctc cttctcttta gactctgatt cttctatctc
    12961 ttctttttct ttgtcagcct ctgaggatgc cactttttct tcagactctt ccttagctga
    13021 ttctgaatct tcctggtctg tttcactctc ctggtcctgt ttatcctctg acttctcaga
    13081 ttcttctccc attcgagctt gtctttcctt ttccttctcc tcagctagag ccgcctcttc
    13141 ttcagccttc ttttttagat attcttggtt tcgtttctgc cattctgata actctttcaa
    13201 ttcttcgagg gtttctttgt cctcattttt cttatctttt gacatttact ttccttatga
    13261 taaatctttt ttcaacaatt gataaaaatc tgctagagat ttcaattcct tagaagcttt
    13321 catcttagct tggtaatctt ccttgtgact tagtaagtga gaaagcttct cttccaaact
    13381 atccaaggtc aaatcgcttt cttgaaggtc ttctgcatag ccttttttta caaagtaagc
    13441 tgcattttca atctggtcac cacgactagc ttcacgacca agcggcacaa tgacatgcaa
    13501 ttttgctatc gccaagagct caaaaatcgt attggcacca cctcgtgtca caacaatatc
    13561 agccaattcc atcaagggtt gatagagatc ggtcacatag tcaacacgaa aaagattttg
    13621 cctcaactca ttcagactag aatctccagt tagattgata atattgtagc gctctgttag
    13681 ttctttctta tggtctgtca ccaattggtt aaagacacga gcgcctgcag aaccgccgac
    13741 aaacaataca gttggcaatt tgggattaaa gtgggtttga atatccacca attcatctgg
    13801 ttctggagtg tttttgtccg aaaccttggt caccgctccc acatgctcga ccttagccaa
    13861 actcgaagct tgttcaaagg ttgaatacat cttagtcgca aatttatagg cgattttatt
    13921 ggccaagccc atagacaggt cagattcgtg aataaagaca ggcactcctg acacacgcgc
    13981 agcgataaca ggcggtactg agacaaagcc cccctttgaa aaaagggtct gtggacgcag
    14041 tcgcaacatg ataaagagcg attggacgat tccccagcca actttgaaga cgtccagcat
    14101 attttgccaa gagaaatagc gacgcaattt cccagtcgca atagaatgga aggtgacatc
    14161 caaacctgac ttaaggattt cttggtgttc gataccacgc ttgtccccga tatagtggac
    14221 ttcccaacca tcttcgatga acttgggcat taacaaaaga ttgagggtaa cgtgtccaac
    14281 cgtcccccca cctgtaaaga caattttttt catattttat tcttttaact ccgctactgt
    14341 gtcgataaag aggtcgccac gtacttcaaa gttagcatac atatcccagc tggcattggc
    14401 aggactaaga agaaccacat ctccttgagt cgcaagttca taggccttac gggtcgcatc
    14461 tgcaatatct gtcgcctcca cataagcgac accagccttg tctgctgccc gtttgacacg
    14521 ttctgcagat tgacccagga tgaccatctt cttgagtcca gtaatgtctg gcaccaattc
    14581 gtcaaactca ttgccacggt ccaaaccacc tgcaatcaag acgaccttgc tgttgtcaaa
    14641 tcctgataag gctttttgag tagccaagat attagttgat ttactgtcgt tatagaattt
    14701 aacacccttg atgtcatcca caaactggag acggtgtttg acaccaccga aggctgaaag
    14761 agtttccttg atggtttgat tgtccacatc acgaagcttg gctacagcaa tagtcgcaag
    14821 ggcattttcc acattgtggc tacctggaac accgatttca ttcgctgcca tgactacttc
    14881 accacggaag tagagttgac catcttccag ataagctcca tcaacctttt caagtgttga
    14941 aaatggtaca acagtggctt ctgtcttgga agtcaagtct tttgccaagt cttgattaaa
    15001 gttcaagaca aggaaatcag ctgctgtcat cttgttctgg atattccact tggctgctac
    15061 atattccgaa aatgacccat ggtagtcgat atgagttggc atgaggttgg taataaccgc
    15121 aatctctgga tggaattctt gaacacccat gagttggaaa gaagaaagtt ccataacaag
    15181 cgtgtcctta tctgatgcta tttgagcaac ctgactagct ggatagccga tattccctga
    15241 taaaagacca tgttgcccag cagcagtcaa aacttcccca atcatagtcg ttgtggttgt
    15301 cttaccgttc gatcctgtga taccaataat cggtgcttct gaaatcaaat aagccaattc
    15361 cacctcagtc aagactggaa ttcccttggc caaagccttt tcaatcatgg gattgttgta
    15421 ggggatacct ggatttttca ccataagggc aaactcttca tccaagagtt ccaaaggatg
    15481 gccacctgta atgaccttga tcccttcttc cagcaaactt tgggcagctg gattgtcctc
    15541 gaaaggtttc ccatcattta ctgtcacaat ggcacctagc ttgtccaaca aacgagctgc
    15601 agattcacca gacttggcca agcctaaaac aaggactttc ttatttttaa attgatctat
    15661 tactttcatg tctcgaactc catttctact cctactattt taccattttt atggaaataa
    15721 aaaagccaca aagtgtgttt gtgactcttt cttctaactg aatcttacca tatcatctat
    15781 gtgataaatc ggtaactcga atgacctgat ccacttgctc ccaaatcaga ggattatggg
    15841 tcgcaataat aatggtccga ttcggatttt ttaaagattc taggatggaa agtaattcct
    15901 cagagttttt ggggtctaag gaagcggttg gttcatctgc gaggatcaaa ggtggatcct
    15961 ttaaaattat cttcgctagt gcaacacgtt gtgcttctcc tcctgataac tcaaatatag
    16021 gttgcttcaa atccaaataa gagaggttta cacggtttag agcttgtttc atcaaagaga
    16081 ttttctcttt ttccttcaac tttttaccaa ctaaacccag attgagattc tctttgacgg
    16141 tttggctttc aattaagcca aaatcttgaa ataagtatcc taagtaatct ctaaagaaaa
    16201 cagaaggctt aatgtcctta agagaagtgc catcatatat gatttgccct ttgtcatatg
    16261 gctctaatcg tccaatcata ttcaagagtg ttgtcttacc acagccactt gtaccgatta
    16321 aggcataaat tttcccacct tcaaaatgaa gattcgtatc tgaaaatagc tgacggcttc
    16381 caaatttttt agatatattc tttagttcaa tcatcctatt ttcctttcat aattgtcata
    16441 gaaacacgag attctttctg cgcttgacgg taaagcgtca aaactgcact aactagaaag
    16501 accaataaag tgagcaagcc aatcaccaag tctcgactgc ttaaaataaa gagactagca
    16561 ccaaatacaa aactggcaaa ttggctaacc atatactgag catgtgtttc aaaaaatcgt
    16621 aaacctgaaa ttcgtttaat caagatatct cggcggaatt gctcgaaata tagaagattg
    16681 acagaataaa agagtaacaa ggaactggct attccaacaa tagctcctaa gattaaagtt
    16741 gctgtttcag tttgaacttc attataacga gttagataaa cacttcttcc ttctttaaga
    16801 taggatactt gctcataaat tccagctttc ttcaagagtt ctagcccact ctcatatcct
    16861 ttgataaaga gttgttttcc agcattgata gaccaactag ataaggatat aaaactatca
    16921 cctgtagaag tcggcgtgaa tacaactaaa atcggatcag tcaaatactg agtagatacg
    16981 ggattctcac cgttattata aacaaaccgc ttttctccca ttgaaagata actaacgtgc
    17041 gctttcatct cataatccaa aggagcactt gcctcctcac cagattttcc ataataactc
    17101 aatctttctt caaaaacttt cttaagttct gcttctcgag agcgcaaatg ttctgggagc
    17161 aagaggataa actcaccttt ttggagatgg gctaacttct gtttggtctc agcatctacc
    17221 acgacctttt ccttgtccaa ataactggga ctaacataga gcgtattagc atctggacta
    17281 taggtatcca gtgtctctcc ctgttcattt tttccttgtg gattggcaaa atggagcaga
    17341 ttatccttta cataaagagc ttgttcttct tcgattgctt ccttggcaaa ggcataccac
    17401 ttgctctgat tttctgtatc ttttcctcta tcacctaagc caaaggaaat ctggtaatag
    17461 tctgctctgt cctgccatgc ttgttttgaa atttcaagtt ctttcaatcg ttggtaagac
    17521 gtcaaacctg tcttaacagc gtagcctact gtaaaaacag ctactaactg acacaatagg
    17581 gttaaagcca tcaagcgttt aaggggtaat cttcccttaa taacgggaac taatgctttg
    17641 taactcaaac tcattaggta aaggagcatt agtaaaattg aaatcgccaa taaaaacaac
    17701 agatagaaac taatcccaaa accataggtg gctaacaaga taggataaaa caaaccttga
    17761 ctaaaaagaa cgactccccc acctaggaag gaaaggaggg ctgatagaag gagccacttg
    17821 atatcagtag ataaacccca attattcacc cctaatctaa aaaccatcca gaatccttgc
    17881 cttagcttag atcctggatg gtttcttttt tcacccaatg ggtgtttttt actagacaaa
    17941 aaagagtttc ccctttatgg tataagtgta gaaaaaaaca caaaaagaaa ggaaactcat
    18001 atgaacagtt taccaaatca tcacttccaa aacaagtctt tttaccaact atctttcgat
    18061 ggaggtcatt taacccagta tggtggtctt atcttttttc aggaactttt ttcccagttg
    18121 aaactaaaag agcggatttc taagtattta gtaacgaatg accaacgccg ctactgtcgt
    18181 tattcggatt cagatatcct tgtccagttc ctctttcaac tgttaacagg ctatggaacg
    18241 gactatgctt gtaaagaatt gtcagctgat gtctactttc caaaattgtt ggaaggaggg
    18301 cagcttgctt cacagccaac cttatcccgt tttctttcca gaactgacga ggaaacagtc
    18361 catagtttgc gatgcctcaa ccttgaattg gtcgaattct ttttacagtt tcaccagcta
    18421 aaccaactca ttgtagatat cgattctacc catttcacaa cttatggcaa gcaagaaggt
    18481 gttgcttata acgcccacta tcgtgctcat ggctatcatc ctctttatgc tttcgagggg
    18541 aagacaggtt attgtttcaa tgcccagctt cgtcctggta atcgttattg ttctgaagag
    18601 gcagacagct ttatcacacc tgttttagaa cggtttaatc aacttctctt tcgaatggat
    18661 agtggctttg cgaccccaaa attatacgat ttaattgaaa aaacagggca atactacctc
    18721 ataaaactca agaaaaatac tgttctgagc cgtcttggag acctttccct cccttgccca
    18781 caggatgaga acttaaccat cttgccccac tccgcctact cagaaactct ctatcaagca
    18841 ggatcttggt cgcacaagcg tcgtgtctgc cagttctctg aacgaaaaga aggaaacttg
    18901 ttctacgatg ttatttctct cgttacaaat atgacgagtg gaacaagcca agaccagttt
    18961 cagctttatc gtggacgtgg tcaagccgag aatttcatca aggagatgaa ggagggattt
    19021 tttggcgata aaacggatag ttcaacctta atcaaaaacg aagttcgtat gatgatgagc
    19081 tgtatcgcct acaatctcta tctttttttc aaacatctag ctggaggtga cttccaaact
    19141 ttaacaatca aacgcttccg ccatcttttt cttcacgtgg tgggaaaatg tgttcgaaca
    19201 ggacgcaagc agctcctcaa attgtctagt ctctatgcct attccgaatt gttttcagca
    19261 ctttattcta ggattagaaa agtcaacctg aatcttcctg ttccttatga accacctgga
    19321 agaaaagcgt cgttaatgat gcattaaaga acagtcgaga tgaaaaaatc gtgtgacgca
    19381 ccaagggagg agtctgccct tttgaggaaa tctagcgagg aaaaacgata ctggaacagc
    19441 agaaagtaaa actgacctca tgaggaggaa gaaagtggct catgaggtca ggggttttgt
    19501 aagttacatc tagttgagag aggtatgaat gatttgggat aaagaatccc ccatgatgga
    19561 taagagagtc tgaccagaaa agagttttat acctgctgct ctcatttcct taatccgagt
    19621 gataatcact aaagcaaaga aagataagcc aaatattgct aaactaatta aaataagggg
    19681 atttagtaat attcgaaaag caagaaaata gggcggtatc tttcggtcag cacttgcttt
    19741 ataacccaaa tctcctaatt tatcggcaag cttttctttc gtcaaggagc ctgacaaaag
    19801 gagataacta tttagcggat cactacgttc acgactttct tggctagctt cttggaattc
    19861 ttttggtaaa gttccctgac cataagttgc ataagtaaag tgagtcgtcc catccttact
    19921 cggctctaca attcttctag ctattaaact ctgttctgag tttgcaaaat tctccaattc
    19981 ctgttcaaat acctcacgcg tcggttcctg agtatctttt ttgacacgaa gtaaagaaac
    20041 ggaatcatag cttgcatata aatattgtgg cgcacgtaag acaataatcc aagcaaggaa
    20101 gaagctgaga aaaaaagttg ataataatat gaatagtttc ttcatagtag actccttgta
    20161 aacaaaattc cccctgtaat ttcttacaag gggaacgatt taaatcaatg aacgattagt
    20221 cataatcaca gtaaaatgct acttgttctc cccatttagt ccaaatccat gcataggagg
    20281 tttttccagc aggagcttct cctttatcag aatgaccatc ccatctgctc acgatggatg
    20341 aataatgata ttttttacca tgatagtaat ttgaaaaagc ctaaccacct cctgaacctt
    20401 ctccatatgt ccatactcct ccatctggat attatacagc agctgatgca gctcccaata
    20461 atgtaaaact tgaaataaga gctagagcaa gtaatctatg ttttttcgtt ttcattttat
    20521 tttttctttc aaaaaaatca caccttgagc aacaatgcaa caaaataaat cctcctctct
    20581 cttttattga aaccgctttc ttgtgtgata agaataacat ttttattatt tgttgtcaag
    20641 gaaaaaatcg aattttttag atgttttact a
//
LOCUS       DBERDK010000034        20420 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.034, whole genome shotgun sequence.
ACCESSION   DBERDK010000034 DBERDK010000000
VERSION     DBERDK010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 20420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20420
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.034"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..164)
                     /locus_tag="ACL73L_001212"
     CDS             complement(<1..164)
                     /locus_tag="ACL73L_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteinase"
                     /protein_id="HGS0772189.1"
                     /translation="MNIKKRVLSAGLTFASALLLAACGQSGSDTKTYSSTFSGNPTTF
                     NYLLDYYADN"
     gene            complement(323..1930)
                     /locus_tag="ACL73L_001213"
     CDS             complement(323..1930)
                     /locus_tag="ACL73L_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HGS0772190.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNGDGVGDLPGITSKLDYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIAAIFGTMEDMDQLIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENTDSPERDYYIWRDEPNDLDSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENEKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQATFGDKDLLTVGETWGATPEIAKLYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELGVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLDQVEDIESLNYAREALEKGVPIEEIMDS
                     IRVIGRDNARTPMQWDESKNAGFSTGQPWLAVNPNYEMINVQEALANPDSIFYTYQKL
                     VQIRKENSWLVRADFELLDTADKVFAYIRKDGDRRFLVVANLSNEEQDLTVEGKVKSV
                     LIENTAAKEVLEKQVLAPWDAFCVELL"
     gene            2083..3567
                     /locus_tag="ACL73L_001214"
     CDS             2083..3567
                     /locus_tag="ACL73L_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HGS0772191.1"
                     /translation="MKKQAFSSEQYLNLQRDHILERINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVGSVVITQYAGQPAADAFLNQLEKNGIDSYLHYPIKGYPTDMDHIISPEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSNMYHDQINGIKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYGETTVNYNRDIEIFPVLKRMLERILGKSPYA
                     SPTDMGVNMVGFAITDDEAAVEASKQEIIRRYYQTVLDFKAEKVGEAAVKKIELLMND
                     LGITPADRKVAVVARQKAEETGGPALAFELPNGEIVTGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPEVVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDAARAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLGINVTFDPYYQYDRLYRK"
     gene            3662..4144
                     /locus_tag="ACL73L_001215"
     CDS             3662..4144
                     /locus_tag="ACL73L_001215"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HGS0772192.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDIISNYDIRLV
                     QPNEDSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAKIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWAKLILEQGISDDAFERHVI
                     "
     gene            complement(4440..6545)
                     /locus_tag="ACL73L_001216"
     CDS             complement(4440..6545)
                     /locus_tag="ACL73L_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGS0772193.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGREVTPEEF
                     AHYRATGQLPGNAETDVQMPQQASGMKQDGVLAKLGRNLAAEAREGKLDPVIGRNKEI
                     QETSEILSRRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISGL
                     EAGTQYRGSFEENVQNLVNEVKEAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAENTFKILQAIRDLYQQ
                     HHNVILPDEVLKAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     ETEKDKQEKAVEAEDFEAALNYKTRIAELEKKIENHTEDMKVTASVNDVAESVERMTG
                     IPVSQMGASDIERLKDMAHRLQDKVIGQDKAVEAVARAIRRNRAGFDEGNRPIGSFLF
                     VGSTGVGKTELAKQLALDMFGTQDAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVIIATS
                     NAGFGYEANLTEDADKPELMDRLKPFFRPEFLNRFNAVIEFSHLTKEDLSKIVDLMLA
                     EVNQTLAKKDIDLVVSQAAKDYITEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     AKHLEADMEDGVLVIREKA"
     gene            complement(7154..8134)
                     /gene="mraY"
                     /locus_tag="ACL73L_001217"
     CDS             complement(7154..8134)
                     /gene="mraY"
                     /locus_tag="ACL73L_001217"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HGS0772194.1"
                     /translation="MFISISAGIVTFLLTLVEIPAFIQFYRKAQITGQQMHEDVKQHQ
                     AKAGTPTMGGLVFLITSVLVAFFFALFSSQFSNNVGMILFILVLYGLVGFLDDFLKVF
                     RKINEGLNPKQKLALQLLGGVIFYLFYERGGDILSVFGYPVHLGFFYIFFALFWLVGF
                     SNAVNLTDGVDGLASISVVISLSAYGVIAYVQGQMDILLVILAMIGGLLGFFIFNHKP
                     AKVFMGDVGSLALGGMLAAISMALHQEWTLLIIGIVYVFETTSVMMQVSYFKLTGGKR
                     IFRMTPVHHHFELGGLSGKGNPWSEWKVDFFFWGVGLLASLLTLAILYLM"
     gene            complement(8136..10388)
                     /gene="pbp2X"
                     /locus_tag="ACL73L_001218"
                     /gene_synonym="pbpX"
     CDS             complement(8136..10388)
                     /gene="pbp2X"
                     /locus_tag="ACL73L_001218"
                     /gene_synonym="pbpX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2X"
                     /protein_id="HGS0772195.1"
                     /translation="MKWTKRVIRYATKNRKSPAENRRRVGKSLSLLSVFVFAIFLVNF
                     AVIIGTGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDE
                     NYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQPNLKQVSFGAKGNGITY
                     ANMMSIKKELEAAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     MESSLNSILAGTDGIITYEKDRLGNIVPGTEQVSQRTMDGKDVYTTISSPLQSFMETQ
                     MDAFQEKVKGKYMTATLVSAKTGEILATTQRPTFDADTKEGITEDFVWRDILYQSNYE
                     PGSTMKVMMLAAAIDNNTFPGGEVFNSSELKIADATIRDWDVNEGLTGGRMMTFSQGF
                     AHSSNVGMTLLEQKMGDATWLDYLNRFKFGVPTRFGLTDEYAGQLPADNIVNIAQSSF
                     GQGISVTQTQMIRAFTAIANDGVMLEPKFISAIYDPNDQTARKSQKEIVGNPVSKDAA
                     SLTRTNMVLVGTDPVYGTMYNHSTGKPTVTVPGQNVALKSGTAQIADEKNGGYLVGLT
                     DYIFSAVSMSPAENPDFILYVTVQQPEHYSGIQLGEFANPILERASAMKDSLNLQTTA
                     KALEQVSQQSPYPMPSVKDISPGDLAEELRRNLVQPIVVGTGTKIKNSSAEEGKNLAP
                     NQQVLILSDKAEEVPDMYGWTKETAETLAKWLNIELEFQGSGSTVQKQDVRANTAIKD
                     IKKITLTLGD"
     gene            complement(10392..10709)
                     /gene="ftsL"
                     /locus_tag="ACL73L_001219"
     CDS             complement(10392..10709)
                     /gene="ftsL"
                     /locus_tag="ACL73L_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HGS0772196.1"
                     /translation="MAEKMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFM
                     QTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIR
                     IAE"
     gene            complement(10721..11671)
                     /gene="rsmH"
                     /locus_tag="ACL73L_001220"
     CDS             complement(10721..11671)
                     /gene="rsmH"
                     /locus_tag="ACL73L_001220"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HGS0772197.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNNYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQAREVKPIETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLKPKMELVSRKPILPSAEELEANNRSHSAKLRVARKIHK"
     gene            11837..12031
                     /locus_tag="ACL73L_001221"
     CDS             11837..12031
                     /locus_tag="ACL73L_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGS0772198.1"
                     /translation="MNRVKEFRKELGISQLELAKDIGVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWEEDF"
     gene            12047..12589
                     /locus_tag="ACL73L_001222"
     CDS             12047..12589
                     /locus_tag="ACL73L_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HGS0772199.1"
                     /translation="MKKETFTEKLIKRTYGISGPLDEYKRREADRIGNQVFIVLFYLM
                     IFGNIIPLLLAYKYPQEVALIYPPLILVIALIASGYVTYQMKKTGITVIEPDMLNEKE
                     SKQLHYPGLKAGLFFGLWMFFITPLLSILIDEGQDYFHSLLTIRNGVSSILGSIFFGA
                     SIQFLISRRIAKTKKNQDED"
     gene            12600..12842
                     /locus_tag="ACL73L_001223"
     CDS             12600..12842
                     /locus_tag="ACL73L_001223"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772200.1"
                     /translation="MKKEKKQLRYPGLKAGSIYGTVIFFIIPLIDTLTSENPNFISSL
                     LNTKHIFKTILGAFFFGVMIHIVDSLRIARAKKDQD"
     gene            12853..13188
                     /locus_tag="ACL73L_001224"
     CDS             12853..13188
                     /locus_tag="ACL73L_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772201.1"
                     /translation="MKSLARLLIIHVFISIFLFFALTSGAISHTVLLLLLLFLPALNK
                     GLEKIQSKRIPVLNAALFFLLISFPQLLTNPVQWKFSIFLVVTIISSLAYFYNFYQVV
                     KEVDQKQLI"
     gene            complement(13298..14299)
                     /locus_tag="ACL73L_001225"
     CDS             complement(13298..14299)
                     /locus_tag="ACL73L_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGS0772202.1"
                     /translation="MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVI
                     HETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQIVKGIEDIASQNGYQVMIGNSN
                     YSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
                     KTNNYDAVYDMTQSCIEKGYEHFLLITADTSRLSTRIERASGFVDALTDANMRHASLT
                     IEDKHTNLEQIKEFLQKEIDPDEKTLVFIPNCWALPLVFTVIKELNYNLPQVGLIGFD
                     NTEWTCFSSPSVSTLVQPSFEEGQQATKILIDQIEGRNQEERQQVLDCSVNWKESTF"
     gene            complement(14359..16260)
                     /locus_tag="ACL73L_001226"
     CDS             complement(14359..16260)
                     /locus_tag="ACL73L_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HGS0772203.1"
                     /translation="MKEERRQFFERVDGNQCRDYILSHCSKDYEKVKSSLERLMDNRF
                     MFDSPWDMESCSKIHQIQPMVWDQVFEDDPEWSYMLNRQEYLLQFMIGYLVEGDKDYI
                     QKCKFFLFDWIEQVREFSPQSLMTRTLDTGIRSFTWLKLLLLLLKFDLLEEKELEKIL
                     VSLEKQIDFMKSHYRAKYTLSNWGILQTIPMLAIYHFFADKMDLEEAYHFASEELKQQ
                     IETQILGDGSQFEQSILYHVEVYKALLDLCLLLPDLQDSYQELLEKMATYIQMMTGLD
                     GRTLAFGDSDSTETTEMLSLSAVVLNKEDLLNGLDVKVDLLSLLFLGREKVKRLQEFE
                     KRAWQPRSMIFEDSGHVCIKDEHRYLFFKNGPLGSAHSHSDENSFCLQYQGQPIFIDA
                     GRYSYREIYERYLLKSAWSHSTCIVDGKAPERITGSWEYEYYPHSLFCHHKEREGVHY
                     IEGAYWSAEPDLPYLHKRKILMLVEDVWLLVDDIRCQGQHEALTQFILDKDVTYQDGK
                     INQLRLWSEVDFDLEDTIISPKYNELERSSKLTKRQFFENQMLDYTIIAHESFEIIRH
                     SVYQTDDREVENALAFEVKNDETDKLILLLSKDICVGEKLCLVDGTKMRGKCLVYDKI
                     NERMIRLQC"
     gene            complement(16282..16575)
                     /gene="yajC"
                     /locus_tag="ACL73L_001227"
     CDS             complement(16282..16575)
                     /gene="yajC"
                     /locus_tag="ACL73L_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HGS0772204.1"
                     /translation="MDTTLFYGIVIVLAVSPLLLSSFHSIRQQKLLRKQMEQRQEYLA
                     SLTSGDEVLLLSGIHGKIISIKDDLVSLQIAKGVVIYVEKESVMGKTKELLFK"
     gene            complement(16575..17393)
                     /locus_tag="ACL73L_001228"
     CDS             complement(16575..17393)
                     /locus_tag="ACL73L_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HGS0772205.1"
                     /translation="MTGSNKLTKRDYLKTSLRAFFCQNGFNYSNYQGLGYANVMYPAL
                     KKHYGEDQEGFYQALEENCEFYNTNPHFLPFITSLHLVMLENGRPTKETRSIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASFAQEGLVVGPILFFLAMNTILTAIKLSTGLYGYK
                     LGTTVIDKLSEQMATISRIANIIGVTVIAGLAATSVKIMVPITFAAGEVKADAKQSIV
                     SIQGMLDKVAPALLPALFTLLVYYLIKEKKWTTYKLVILTVIIGIIGSWLKIIA"
     gene            complement(17380..18159)
                     /locus_tag="ACL73L_001229"
     CDS             complement(17380..18159)
                     /locus_tag="ACL73L_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HGS0772206.1"
                     /translation="MSINVFQAILIGLWTAFCFSGMLLGIYTNRCIVLSFGVGIILGD
                     LPTALAMGAIGELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVSPEAALALST
                     PIAVAIQFLQTFAYTVRAGAPETAMKHLKNHNLKKFKFTLNATIWLFAFIGFTLGCLG
                     ALSMDTLLKLVDYIPPVLLTGLTVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLNIPTIGIAIVGTIFALIEFYNKPKTADHVVEEEAHDDWI"
     gene            complement(18174..18665)
                     /locus_tag="ACL73L_001230"
     CDS             complement(18174..18665)
                     /locus_tag="ACL73L_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGS0772207.1"
                     /translation="MTMPNIIMTRIDERLIHGQGQLWVKYLGCNTVIVANDEVSTDKM
                     QQTLMKTVVPDSVAMRFFPLQKVIDIIHKANPAQTIFIVVKDVKDALTLVEGGVPIKE
                     INIGNIHNAPGKEQVTRSIFLGEEDKAALKELSQTHQVTFNTKTTPTGNDGAVQVNIM
                     DYI"
     gene            complement(18676..19866)
                     /locus_tag="ACL73L_001231"
     CDS             complement(18676..19866)
                     /locus_tag="ACL73L_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HGS0772208.1"
                     /translation="MIKKVTIEKIKSPERFLEVPLLTKEEVGQAIDKVIRQLELNLDY
                     FKEDFPTPATFDNVYPIMDNTEWTNGFWTGELWLAYEYSQQDAFKNIAHKNVLSFLDR
                     VNKRVELDHHDLGFLYTPSCMAEYKINGDGETREATLKAADKLIERYQEKGGFIQAWG
                     DLGKKEHYRLIIDCLLNIQLLFFAYQETGDQKYYDIAESHFYASANNVIRDDASSFHT
                     FYFDPETGQPFKGVTRQGYSDDSCWARGQSWGVYGIPLTYRHLKDESCFDLFKGVTNY
                     FLNRLPKDHVSYWDLIFNDGSDQSRDSSATAIAVCGIHEMLKHLPEVDADKDIYKHAM
                     HAMLRSLIEHYANDQFTPGGTSLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDW
                     NLYW"
     gene            complement(19878..20312)
                     /locus_tag="ACL73L_001232"
     CDS             complement(19878..20312)
                     /locus_tag="ACL73L_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGS0772209.1"
                     /translation="MKIVLVGHGHFATGIYSSLQLIAGNQENVEAIDFVEGMSADELK
                     QKILLAISNEEEVLILSDLLGGSPFKVSSTIMGENPAKTMNVLSGLNLAMLMEAVFAR
                     MAHSFDEVVNKSVVAAQGGVVNGKELFSTDAEEEEEDFESGI"
BASE COUNT         5938 a         4643 c         3670 g         6169 t
ORIGIN      
        1 atattatcag cgtaatagtc taatagatag ttaaatgtag ttggatttcc actaaaggtt
       61 gatgagtaag tttttgtatc tgaacctgat tggccgcaag cagctaaaag caaagcagat
      121 gcaaaagtca ggcctgcact aaggacacgc ttctttatgt tcatcttctt tctccttaat
      181 agtgggaatt tgtaaagtta attgaatttc aagaatgaaa gtttataaac tttggttata
      241 aaaaacaaag gatttctgtc ttttatacag tcctcccttg tttttatacg atttcaattt
      301 taaatttttc tgcaaaaaat atttatagta attccacaca gaaagcatcc catggagcca
      361 agacctgttt ttcaagtact tctttagccg cagtgttttc aatcaagaca gatttgactt
      421 ttccttctac tgtcaagtct tgctcttcat tggacaagtt agccacaact aggaagcgac
      481 ggtcgccatc cttacgtata taagcaaaga ccttatcagc cgtatcaagc aattcaaagt
      541 cagctcgaac tagccagcta ttctccttgc gaatttggac cagtttctga taggtataga
      601 aaatagaatc tggatttgcc agcgcttctt ggacattgat catctcgtaa tttggattaa
      661 ccgccaacca aggttgacct gttgagaaac cagcgttttt gctctcgtcc cattgcatag
      721 gggtacgggc attgtcacgt ccaataacac ggatactgtc catgatttct tcaatcggaa
      781 cacctttttc aagagcctca cgcgcatagt tgagagattc aatatcttct acttgatcca
      841 gtgtttcaaa cggatagttg gtcatcccaa tctcctcacc ttggtagata taaggagttc
      901 ctctcataag atgaagcaag attgcaaagg ctttggcaga tttttcgcgg tattcttggt
      961 catttcccca gattgagacg atacgaggga ggtcatgatt gttccagaag agggaattcc
     1021 aaccgtcctc aactcctaac tctgtctgcc atttgttgaa gatttctttt aacttagcga
     1081 tattcagctc tttttgatag tgccatttag gctgaccttc ctgatactga agaccgatat
     1141 gttcaaactg gaagaccata gacaattctt gcccctttgg atcagagtaa agcttggcaa
     1201 tctctggcgt tgctccccaa gtctccccta ctgtcaagag atccttatct ccaaaggtcg
     1261 cctgattcat ttccttgaga tagggatgga gcataggacc attattgact accttctcgt
     1321 caggaatttt gccaatcatg tcaataacat ccatacggaa accaccaata cctttatcaa
     1381 tccagaagtt catcatctca taaattttct ggcgaagttt ttcattttcc cagttgagat
     1441 ccggctgttt cttgctgaaa aagtggagat agtattgacc tgacttttca tcgtattccc
     1501 aagcagaccc actaaagata gaatctaggt cattgggttc atcgcgccag atatagtagt
     1561 ctcgctcagg gctgtcagta ttttcacagg cttcgacaaa ccaagcatgt tcatctgagg
     1621 tatgattaac caccaagtcc atgatgatac gaatgtcacg cttcttagct tctgcaatca
     1681 gctgatccat gtcctccatg gttccaaaaa tagccgcaat cgcttgataa tcagcaatat
     1741 catagccatt atcatccata gggctgtcat aaacgggaga aagccaaatt gctgtgattc
     1801 ctagcttggc tagatagtcc aacttactgg taatacctgg caaatcacca actccatctc
     1861 cattactatc cataaaactc tttggataga cttgatagac tacggcattg tgccaccatt
     1921 tttcttgcat ctgtttacct cattatttta ataatctata gtatatgata gcacttttta
     1981 aaattttgtg caagcgtttg cttaatcttt ttaatttatt ttttagctcc gtagtttcct
     2041 aacttccaat tttttattat aatagagtcc agaggtaaaa aaatgaaaaa acaagctttt
     2101 agttctgaac agtatttgaa tttacaacgc gaccatattt tggagcgcat taaccaattt
     2161 gacggcaagc tctacttaga gtttggcggt aaaatgttag aagatttcca cgctgctcgt
     2221 gtccttcctg gttatgaacc tgacaacaaa atcaagctct tgcaagaatt gaaagagcag
     2281 gttgaggttg tgattgccat taatgctagc aacattgaac attccaaagc acgtggcgac
     2341 ttgggcattt cttatgacca agaagttctt cgtttgattg acaaattcaa tgaattaggg
     2401 atttttgttg gttccgttgt cattacacaa tacgctggcc aacccgctgc agatgccttc
     2461 ctcaaccaac ttgagaaaaa cggaattgat tcttatcttc attatccaat caaaggatat
     2521 ccgacggata tggatcacat catttcccca gaaggcatgg gcaaaaacga ctacatcaaa
     2581 accagtcgca acttgattgt cgtaaccgct cctggacccg gttctggaaa attggcaacg
     2641 tgtatgtcca atatgtacca cgaccaaatc aatggcataa agtctggcta cgctaaattt
     2701 gaaaccttcc ctgtttggaa tcttcccctt catcatccag ttaacttggc ttatgaagct
     2761 gccacagctg accttgatga tgttaacatg attgacccct tccatcttca aacctatgga
     2821 gaaaccactg tcaactacaa ccgtgatatc gaaatcttcc cagtgctcaa gcgcatgttg
     2881 gaacgtattc tcggaaaatc accatacgct tcaccgacag atatgggtgt caacatggtt
     2941 ggtttcgcta ttacagatga cgaggctgct gtcgaagctt ctaaacaaga aatcatccgc
     3001 cgttactatc aaacagttct tgacttcaaa gctgaaaaag ttggcgaagc tgccgtcaag
     3061 aaaattgagt tgctcatgaa cgacctcggt atcacacctg cagaccgtaa ggttgctgtc
     3121 gttgcgcgcc aaaaagcaga agaaactggt ggaccagccc tagcctttga attgccaaat
     3181 ggggaaatcg tcactggtaa gaactcagaa ctctttggcc ctacagccgc tgccttgatc
     3241 aacgccatca aaaaatcagc tgacatcgct aaagaagtaa aactaatcga gcctgaagtt
     3301 gttaagccaa tccaaggtct taaaatcgat catctcggta gccgcaatcc acgccttcat
     3361 tcaaatgaaa tcctgattgc acttgctatc acagctacag aaaatcctga tgctgcccgc
     3421 gctatggaag aactcggcaa cctcaaagga agcgaagccc actcaaccat catcttgact
     3481 gatgaagaca agaatgtcct tcgtaaactg ggtatcaacg taacctttga cccatactac
     3541 caatacgacc gcttatatcg taagtaaaga tttcaacctc tctagcctcg gagaggtttt
     3601 ctctctgtct caggaacaac ctttatgata taatgagaga agaaaactga aaggatttac
     3661 catgtcaaaa gaagttattg tcgaaagttt tgaacttgac cacaccattg tcaaagcacc
     3721 ctatgttcgc ttgattgggg aagaaacagg accaaaagga gacatcatct ccaattatga
     3781 tattcgcttg gtgcaaccaa acgaagactc tatccctact gccggccttc acactatcga
     3841 gcacctctta gccaaactca tccgtacccg cattgacggc atgattgact gttcaccatt
     3901 tggttgccgc acaggcttcc acatgattat gtggggacgt cacaccagtg ctaaaatcgc
     3961 agctgttatc aaggattcgc tcaaggaaat cgctgaaact actacttggg aagatgtccc
     4021 agggacaacc atcgaatctt gcggaaacta caaggaccac agcctcttct ctgctaaaga
     4081 atgggcaaaa ctcatcttgg aacaagggat ttcagatgat gcctttgaac gtcatgtgat
     4141 ttaaaatcga gtaggggata atctctagcc cttctcacac caccgtacgt gccgttcggc
     4201 atacggcggt tcaactaact tttaacgcat gtcgttcaag gtaataatcc aaacacgaaa
     4261 ccagtccacg tttctcaagg actggttttg atatagcacg tttaagtacc gacttctgag
     4321 ctactatagt agattgaaac tagaataata cacttctact atacgacatg cccgtcgtac
     4381 ctcaaaagga accagttttt ttcagctggc tccttctttt ttatcctcaa aattctatct
     4441 taggctttct cacgaataac caaaacgcca tcttccatat ctgcttccag atgtttagca
     4501 tctaaatgat ccaagtggaa gtctgtcacc ttatcacgaa tttcttgttc aaccactcga
     4561 cggagaggac gaactcccat gacttcgtcg taaccttctt ctgtgatata atctttagcc
     4621 gcttgactga ctaccaagtc aatgtctttc ttagccaagg tttggttaac ttcagccaac
     4681 atcaaatcta caatcttaga aaggtcttcc ttagtcaagt gtgagaattc gatgactgca
     4741 ttaaagcggt tgaggaattc tggacggaag aagggtttca aacggtccat caattctggt
     4801 ttatccgcat cttctgtcaa gttggcttca tagccaaatc cagcatttga ggtcgcaata
     4861 atgacagtgt tcttgaagtt tactgtattt ccttgaccat ctgtcaaacg accatcatct
     4921 agaacttgga ggagaagggt aataacttga gggtcagcct tttcaatttc atccaagaga
     4981 atgatagagt atggattgcg acgaacacgt tctgttaagg tattgctatt gtcatcataa
     5041 cccacatagc ctgctgttgt accaattagc ttagaaacag ctgtgcggtc actgtattca
     5101 gacatatcca aacggataat cgcatcctgg gttccaaaca tatcgagtgc caattgctta
     5161 gcaagctccg tcttaccaac cccagtagac cctacaaaga ggaagctgcc gattgggcga
     5221 tttccttcat caaaaccagc acggttacga cggatagcac gagctacagc ttctacggcc
     5281 ttatcttgac caatcacctt gtcttgcaag cgatgagcca tatctttcaa acgttcgata
     5341 tctgaagctc ccatttgcga tactgggata cctgtcattc gttccacaga ttcagccaca
     5401 tcgttgacac ttgcagtcac tttcatatct tctgtgtggt tttcgatttt cttttccaat
     5461 tctgcaatgc gtgttttata gtttagagct gcttcaaaat cttctgcttc aactgctttt
     5521 tcttgcttgt ctttttccgt ttcgatttct cgttcaacag catgcacatc tgttactgga
     5581 tgttgagccg ccaagtgagc agccgttaca tcgacaaggt caatagcctt atctggcaag
     5641 ctacgttgag gaatgtattg aacagaataa tccaccgctg ctttcaagac ttcgtctggc
     5701 aagatgacat tgtggtgttg ttgatagagg tcacgaatgg cttgaagaat tttaaaagta
     5761 ttctctgctg aaggagcatt gaccttcact tcgttgaaac gacgagcaag agcagcattc
     5821 ttcaagatgg tgttacggta ttcgtcttga gttgttgccc caatcactgt caattctcca
     5881 cgagagagag ctggcttgag aatatccgca agtcctttag aaccactgtc tccaccagtg
     5941 ctaccagcac caagaatttg gtgaatttca tcaaagaaga ggataatatt ccctgcttct
     6001 ttcacttcat tgactaagtt ttggacattt tcttcaaagc taccacggta ttgagtacca
     6061 gcctcaagac ctgagatatc aatagaaata atttccttgt tcttgatagc agcaggaaca
     6121 tctccgttca caatggcttg cgctagacct tcgacaactg ctgtcttacc aacacctgca
     6181 tctccgacca aaacaggatt gttcttggtg cgacgtgaga ggatttcaga tgtttcttga
     6241 atttccttgt ttcgtccgat aacaggatcc aacttgccct cacgcgcttc tgctgccaag
     6301 tttcgaccta gttttgcaag gacaccgtct tgtttcatac ctgatgcctg ttgtggcatt
     6361 tgcacatcag tttctgcatt tcctggtaat tgaccagtcg cacgatagtg agcaaattcc
     6421 tcaggtgtga cttcgcgtcc attaattaag taacggcgat tttcagaact atatcctcgc
     6481 ataccaccca tcaattggtt aaataaatca tccatgttat taaaattatt aaagttgtta
     6541 ttcatattct ttacctcttt ttgtttactt agttatgact actgatattg actatctttg
     6601 accattgttt taaaaaattt agactagcta aaccgctacc ctacgtacta tttctggttt
     6661 ttctttttca agcaggagta gactatcttt acttctgaaa atttctagat gaaacataga
     6721 cccacctctt tctattttac tttaaataag tgttctagta aggctttcat ttaccttacg
     6781 ttcataatat actacattgg tcagaaaagg tcaagagttt tgactttaac tgaccaatat
     6841 ttttttacac tcttcgaaaa cctcttcaaa ccacatcagc ttctccttat ctacaacctc
     6901 aaaaccatgt tttgagctga cttcgtcagt tctatcaacc atctcaaaac tatatattaa
     6961 aatgaaataa gaacaggaca aatcgatcag gacagtcaaa tcgatttcta acaatatttt
     7021 agaagcagat gtgtactatt ctagtttcaa tctactatat tttgagcagc ctaaagttag
     7081 ctttctagtt tgttctttga ttttcattgt gtatttaaat acaaaaacac cctgaaacat
     7141 cagggtgcca ttcttacatt aaatacaaaa ttgctagggt caggagactt gctagaagtc
     7201 ccactcccca aaagaagaag tcaaccttcc actcgctcca aggatttcct ttaccagaca
     7261 atcccccaag ctcaaaatgg tgatgtacag gcgtcatacg gaaaatacgt ttaccacctg
     7321 tcagtttgaa ataactgact tgcatcataa cagaagttgt ttcaaaaaca tatacaattc
     7381 cgataatcaa gagagtccat tcttggtgga gagccataga gatagctgcc agcatcccac
     7441 ctagggccaa acttcccaca tcacccataa agaccttggc aggcttatgg ttaaagatga
     7501 agaaaccgag caaaccacca atcatggcaa gaatcactag aagaatatcc atctgacctt
     7561 gcacataggc aataactcca taggcagaca aactaatcac aacggaaata ctagctaaac
     7621 cgtcaacacc gtctgtcaag tttactgcgt ttgaaaaacc gactagccag aaaagagcga
     7681 agaaaatata gaaaaatccc aaatgaactg gataaccaaa gacagacagg atatcgccac
     7741 cgcgctcata gaaaagatag aagataactc cacctagaag ctgaagagct aatttttgct
     7801 taggattaag cccctcattg attttacgaa agaccttgag aaagtcatct aaaaatccga
     7861 ccaagccata caagaccaag atgaacaaaa tcattcccac attattgctg aattggctac
     7921 taaatagggc gaaaaagaaa gcaaccaaaa cagaagtaat caagaaaacc aaacctccca
     7981 ttgtaggagt cccagctttt gcctgatgct gtttgacatc ctcatgcatc tgctggcctg
     8041 taatttgcgc ctttctataa aattggataa aggccggaat ttctactaaa gttagtaaaa
     8101 atgtcacaat tccagcactg atggaaataa acatattagt ctcctaaagt taatgtaatt
     8161 tttttaatgt ccttgatagc tgtgttagca cgaacatctt gcttctgcac agtagagccc
     8221 gaaccttgaa attcaagttc tatattgagc cacttagcaa gggtctcagc agtctccttt
     8281 gtccaaccat acatatctgg aacctcctct gctttatcag ataagataag gacttgctgg
     8341 ttcggggcaa gattcttccc ttcttcagca gaactgtttt taatcttcgt tcctgttccc
     8401 acaacgatgg gttgtacaag attgcgacgc aattcttctg ctaaatcacc aggtgaaata
     8461 tccttgacac taggcatagg ataaggactt tgttgactta cttgctctaa agccttagct
     8521 gttgtttgaa gattgagaga gtctttcata gctgaagccc gctccaagat aggattggca
     8581 aattctccca actgaatacc tgaataatgt tcaggttgtt ggaccgtcac atacaagata
     8641 aaatcaggat tttcagccgg actcatcgat acagccgaga aaatatagtc ggttaacccg
     8701 actagataac caccattttt ctcgtcagca atctgagccg taccagactt gagggctaca
     8761 ttttgcccag gaacagttac agttggcttg cctgtgctgt ggttatacat ggttccataa
     8821 accggatccg tccctaccaa aaccatgtta gtccgagtta gactagctgc atctttagaa
     8881 acaggatttc ccacaatttc tttttgagat ttccgagcag tttgatcatt tggatcataa
     8941 atggcactaa taaatttagg ctccagcatg acaccgtcat tagcaatagc tgtaaaggca
     9001 cgaatcattt gcgtctgggt cactgaaatc ccttgtccaa atgagctttg cgcaatgttg
     9061 acaatattat ccgcaggaag ctgaccagca tactcatccg tcaaaccgaa acgggtcgga
     9121 actccaaatt taaaacgatt aagataatca agccaggtag catctcccat cttttgctca
     9181 aggagagtca tcccaacgtt acttgagtgt gcaaaacctt gagaaaaagt catcattctg
     9241 ccaccagtca atccttcatt aacgtcccaa tctcgaatcg tggcatctgc aatttttaac
     9301 tcactactat taaagacttc tcctcctgga aaggtattat tatcaatagc agcagccaac
     9361 atcatcactt tcatagtgga acctggctca tagttacttt ggtaaaggat atcacgccaa
     9421 acaaagtcct ctgtaatgcc ttcttttgta tctgcatcaa aggtcggtcg ttgcgttgtt
     9481 gccagaattt cccctgtttt agcactgacc aaagtcgctg tcatgtactt tccttttacc
     9541 ttctcttgaa aagcatccat ctgggtttcc ataaaggact ggagggggct ggaaatggtt
     9601 gtataaacat ccttaccgtc catcgttcgt tgggaaactt gttctgttcc gggtacaata
     9661 ttacccagac gatccttttc ataggtaata atgccgtctg tccctgcaag aatactgttc
     9721 aaggaactct ccattccaga ggttcccagc aagctcttgc ttccatcttc attttcatgg
     9781 agctgagcta ggccgataaa actagaagca aattgtccgt ttgggtaact acgattggga
     9841 ctggttgtaa aatcaatccc cttgacctct gcagcttcca attctttttt gatagacatc
     9901 atattggcat aggtaatccc atttcccttt gctccaaagg aaacttgctt gagattaggt
     9961 tgcgagagtt gctctcttac ataggattct tccatgtcca gatacttatg aaagacctct
    10021 gcaaccttgt taaattgtgt tttttctacg taaagaatct tacccgttgc tgacttatag
    10081 ttctcatcaa tgaccgcata gacattatag gaggttgcat cctcagcaat cgggactcca
    10141 tttcggtcat aaatagtccc acgtttggca ggaactgtac gggtggtttg atgaaccttc
    10201 ttagcttcct tcgctaaatc tgttccaaag cgagtgcctg tcccaataat gaccgcaaaa
    10261 ttgactaaaa aaatggcaaa aacaaagaca gataataaac tcagactttt tccaactctg
    10321 cgtctgtttt cagccggcga tttccgattt ttggtcgcat aacggattac tctttttgtc
    10381 cacttcatat cttactccgc tattctaata ttttcattgt ttaattgcaa atcgtgtgaa
    10441 ttggcaattt ctttcaaacg ttctgcacgt aatagttcat tgacctcttg cttggcatcg
    10501 tccaattcgg tcttcttttc ctctatctgc gcattgattt ttgtcaaatc attctgcact
    10561 tgcaagagct tggtctgcat aaaaataata ctaatggcta caataagagt ggttacagca
    10621 atggaaaagt aaaaagcttt ttccacacgc gaaaaccgtt taagttgcat ctgtagtatt
    10681 tgacctgttt tttccatttt ttctgccatc tttttccctc ttacttgtga atttttctgg
    10741 ccacgcgcaa cttggctgag tgcgagcggt tattggcttc taactcttcc gcacttggca
    10801 agattggctt acgggacacc aattccatct tgggcttgag atcatctggg atgaaaggca
    10861 agccttttgg aacttcaact gttgaagctt ccttgaacaa ttgcttggtc aagcggtctt
    10921 ctaaggaatg aaaggtaatc actgaaattc taccatccag agccaacata tccatagcct
    10981 gctggatgga ctcatctgcc gcccccagtt catcattgac ttcaattcga atagcctgga
    11041 aaatctgctt agcaggatgc cccttcttct tgagttcctt ggcaggtttg accaacttga
    11101 taatctctgc taactcagtc gttgtctcaa tcggcttcac ttcacgcgct tgctcaatct
    11161 tacgcgcaat ctgtttagag aatttgtcct ctccatactt gaagaaaata cgaaccaagt
    11221 catgatagtc ataattgttc accacttcat acgctgtcag actagcatcc tgattcatcc
    11281 gcatgtccag tggcgcatcc tttttataag aaaaaccacg ctcacgctgg tctaattgag
    11341 gactagacac tcccaagtca taacaaattc catcaatttc ctgaacacca gcttcgcgca
    11401 aacgtgcctg taaatgacgg aagttgtcct tgataaaggt caccattccc ttctcaatgt
    11461 aaggtgccaa gcgtttttgc gcattgtcaa tggcattctg atcctggtca aaggcataga
    11521 gatggccttt ttcacttaat ttacttaata aatactcgct atggcctgct ccacccaaag
    11581 tcgcatcaac gtagataccg tcaggtttta cgtcaagcat atcaatcgtt tcgtggagta
    11641 agaccgttac atgatgaaat tcttttgtca tatcttatct attttaccac aaatccgacc
    11701 agcttgcact tgtcagacaa ataggacaag gagactacta tttcttcaaa aaatatgtca
    11761 aaaatacttg acactttatg tttaaaagac tataatatag tcaaatatat acgacataaa
    11821 tcagaaagga gaccagatga atcgtgtgaa agaatttcgc aaggaattgg gtatttccca
    11881 gctcgaactc gccaaggata tcggtgtctc gagacagact atcaatatga ttgaaaacga
    11941 caagtacaat ccaaccttgg aactctgtct caatctcgcc cgcagcctcc aaactgacct
    12001 caacagtctc ttttgggagg aagattttta aaaaaggagc aaactcatga aaaaagaaac
    12061 cttcactgaa aaactgatca aacgcacata cggtatttct ggtccccttg acgaatacaa
    12121 acggcgtgag gccgatcgta ttgggaacca agtctttatc gtcctctttt atctgatgat
    12181 tttcggaaat attattccac tccttctggc ctataaatac cctcaagaag tggctctaat
    12241 ctatcctcct ctgattttag tgattgccct catcgcttct ggctatgtca cctaccaaat
    12301 gaaaaaaaca ggcatcacag tcattgaacc agatatgctg aatgagaaag aaagcaagca
    12361 actacactac cctggtctga aagcaggttt gttctttggt ctatggatgt tttttataac
    12421 tcctcttctc agtatactca tagatgaagg tcaggactat tttcattctc ttctcactat
    12481 aagaaatggt gtatcaagta ttctcggttc tattttcttc ggagcgagca tacagttcct
    12541 catctcccgt cgcattgcaa aaactaagaa aaatcaagat gaggattagg aggaaactca
    12601 tgaaaaaaga aaagaaacag ttacgttatc ctggtctgaa agcaggttcg atttatggaa
    12661 cagtgatatt ttttataatt ccactcattg ataccctaac aagtgaaaat ccaaatttta
    12721 tcagttctct tctaaataca aaacatattt ttaaaactat actgggagct ttctttttcg
    12781 gagtgatgat acatattgtc gactcacttc gtattgcaag agctaaaaaa gaccaggatt
    12841 aggaggtgcc ttatgaaatc actagctaga ctactgatca ttcatgtttt tatcagtatt
    12901 ttccttttct tcgcccttac ttcaggagct atttctcata cagttttact actcctactc
    12961 ctctttcttc ctgcgctcaa taaaggactt gagaaaatac aatcaaaacg gatacctgtc
    13021 ctcaacgcag ccctcttctt tctcctcata tcctttccac aacttttaac caaccctgtc
    13081 caatggaaat tttcaatatt cctagtcgta accatcattt caagtttggc ctacttctat
    13141 aacttttatc aagtagttaa agaagtagat caaaaacagt tgatttagga gcttgctccc
    13201 ataatttctc attttatagg caaataatcc cacctagctt gttctaggtg ggattatttt
    13261 atttcttaac agagattgca agtcattttc cttcatttta gaaagtcgac tctttccaat
    13321 tcacactaca atccaagact tgttgccttt cttcttgatt gcgaccttca atctggtcaa
    13381 tcaaaatctt tgtagcctgt tgtccttcct caaaggaggg ctgaaccagc gtcgaaacac
    13441 ttggagaaga aaagcaagtc cactccgtat tgtcaaaacc aatcaaccca acttgtggca
    13501 agttataatt caactctttg ataacggtaa agactagagg tagggcccaa cagttaggga
    13561 taaataccag agttttttca tcgggatcga tttctttttg taaaaattcc ttaatttgtt
    13621 ccaaattcgt atgcttatct tcaatggtta gactggcgtg gcgcatatta gcatctgtta
    13681 aagcatccac aaaaccactt gcccgctcaa tccgagtact caaacgactc gtatccgctg
    13741 taatcaagag aaaatgctca taaccttttt cgatacagga ctgggtcatg tcataaacgg
    13801 catcatagtt attggtttta acccagctag tccggtgttc atagagctga ctatcaaaaa
    13861 agaccatttt cttctttttc tcatcgatga tacgagaata ttttcggaaa ttagaggtcg
    13921 gctgaataat aaagccgtct actcccaaga gaagcatgct ttcaatatac cggtcctcac
    13981 tctcttggct gtaattacta tttcctatca ttacctggta gccattctgg ctggcgatat
    14041 cctcaattcc cttaacaatt tggtttgaga aactgttggt aatatcaccg atcaaaacac
    14101 cgattaattt tgttcgtttg gagtttaagc tacgcgcaac aatgctcggt ttgtaatttg
    14161 tttcatgaat aactttttca atcttttcac gtgtctcttg ggacattttt tcatatttcc
    14221 cgtttaggta aaatgacacg gttgttttcg aggtctgagc catttccgca atgtctttta
    14281 tggtcagttt cttctccaat ctaaaacacc tcctactaat acttattata acatattcac
    14341 tattcaaaat gcctatttct agcactgcaa gcgaatcatt ctctcattta ttttatcata
    14401 tactagacat tttccacgca tttttgttcc gtcaacgagg cacaattttt cacctacaca
    14461 aatatccttg cttaataaca gaatcagctt gtctgtttcg tcatttttca cttcaaaagc
    14521 cagagcattt tccacttcac gatcatctgt ctggtagaca gaatgacgga tgatttcaaa
    14581 actctcatgc gcaatgatgg tataatccag catctgattc tcaaagaatt ggcgcttggt
    14641 gagtttgcta cttctttcaa gctcattgta tttaggagaa atgatggtat cttccaaatc
    14701 aaaatcaact tcactccata gtctcaactg attgattttc ccatcttgat aggtcacatc
    14761 cttgtcaagg ataaactgag tcaacgcctc atgctgacct tgacacctga tgtcatctac
    14821 caagagccag acatcctcta ccaacatgag gatttttctc ttgtgaagat aaggcaaatc
    14881 aggttctgct gaccaataag ccccttcaat ataatgcact ccctcccttt ctttatggtg
    14941 acaaaacagg gagtgaggat agtattcata ttcccaggat cccgtgattc tttccggagc
    15001 tttcccatct acaatgcagg tcgaatgact ccaagcactc tttaagagat aacgttcata
    15061 tatctcccga taagaataac gcccagcatc tatgaaaata ggttggcctt gatactgtaa
    15121 gcaaaaacta ttctcgtcac tatggctatg ggcacttcct agcggaccat ttttgaaaaa
    15181 tagataacga tgttcatcct taatgcagac atgtccagag tcttcaaaga tcatggacct
    15241 aggctgccaa gctctctttt caaattcctg cagtcgcttg accttttctc gccccaggaa
    15301 caagaggcta agcaaatcga ctttaacatc cagaccgtta agaaggtctt ccttgttcaa
    15361 aaccacagca gacaggctca acatttctgt cgtttctgta gaatcgctat caccaaaagc
    15421 caaagttcgt ccatctaagc ctgtcatcat ttgaatatag gtcgccatct tttccagcaa
    15481 ctcttggtaa ctatcttgca agtctggaag caagagacac aaatccagca aggctttata
    15541 aacctctaca tgatagagaa tcgactgttc aaactggctt ccatctccta aaatctgtgt
    15601 ctcaatttgc tgtttcaact cctctgaagc aaaatggtaa gcttcttcta ggtccatctt
    15661 atctgcaaag aaatgataga tagcaagcat cggaattgtt tgtaaaattc cccagttact
    15721 aagggtgtac ttggcgcgat agtggctttt cataaagtca atctgctttt ctagactgac
    15781 caaaattttc tctagttctt tctcctctag caagtcaaat ttcaagagga gcaagagtag
    15841 tttcaaccaa gtaaaggaac gaatacccgt atccaaggtt ctagtcatca aggattgagg
    15901 agaaaattct ctcacctgct caatccaatc aaatagaaag aacttgcact tttgaatata
    15961 gtccttatct ccttctacca gataccctat cataaactgc aagagatatt cttgtcgatt
    16021 gagcatataa gaccattctg gatcatcttc aaatacttga tcccatacca tcggctggat
    16081 ttgatggatt tttgaacaag actccatatc ccaaggacta tcaaacataa aacgattgtc
    16141 catcaagcgt tcaagggaac tcttgacttt ctcatagtct tttgaacagt gggacaagat
    16201 ataatcacga cattggtttc catcgactct ttcaaaaaat tgtcttcttt cttctttcat
    16261 tatctattac cagaaaaaga actacttaaa aagcagttct tttgtctttc ccattacact
    16321 ttccttttct acatagatga ccacaccttt tgcaatctgc aaggagacca agtcatcttt
    16381 gatagaaatg atttttccat gaattccaga caataacaac acttcatcac cagatgttaa
    16441 agaagctaaa tactcttgtc gttgctccat ctgtttgcga agcaactttt gctgacgaat
    16501 agaatgaaag cttgacagta aaaggggact cactgccaag acaatcacta ttccataaaa
    16561 caatgttgta tccattaagc tataatctta agccagcttc cgataattcc gatgataact
    16621 gttaaaataa cgagtttata tgttgtccat ttcttttctt tgatcaagta gtaaactaaa
    16681 agtgtaaata gggctggtag aagagctgga gcaaccttat caagcattcc ctgaatactt
    16741 acgatacttt gtttagcgtc tgctttaact tcccctgcag caaaggtaat cggcaccata
    16801 atcttaacag atgtcgctgc caaaccagca attacggtta caccgataat attggcaata
    16861 cgagaaatcg ttgccatctg ctcgcttagt ttatcaatca cagttgttcc tagtttgtat
    16921 ccatacagac cagttgacaa tttaatcgct gttaaaatcg tattcatcgc aaggaagaac
    16981 aagattggac cgacaaccaa gccttcttga gcaaacgaag ctgcgatggt tgagaacaat
    17041 ggagctaaac agaattgaga aagagaatcc ccaatacctg ccaatggtcc catcaaggcc
    17101 atcttgatgc tacgtgtttc ttttgttggt cgaccatttt ccaacattac aagatgcaag
    17161 ctggtaataa aaggcaggaa gtgtgggttg gtattataga attcacagtt ttcttccaag
    17221 gcttggtaga aaccttcctg atcctctcca tagtgttttt tcaaagcagg atacatcaca
    17281 ttggcatatc ccaacccttg atagttacta tagttaaatc cattttgaca aaagaatgcc
    17341 cgcaaagacg ttttaagata atcacgtttt gttaatttgt tagatccagt catcgtgtgc
    17401 ttcctcctct accacatgat ccgctgtttt tggcttgtta taaaattcaa tcaaagcaaa
    17461 gatagtacct acaattgcaa taccaattgt tgggatgttt agataagctg cacaaacata
    17521 tcccaacaag acaaagggaa tcaactcttt cttagccatc actgacaaga tcatcgcaaa
    17581 accgatagct gggagcattt taccagcaac tgtcaaacct gtaagtaata ccggtggaat
    17641 gtagtctacg agtttcaaca aggtatccat tgaaagggca cccaagcaac ccaaggtaaa
    17701 tccaataaag gcaaacaacc aaattgttgc atttagagtg aacttgaatt tcttcaaatt
    17761 atggtttttc aagtgcttca tcgccgtttc aggcgcacca gcacgtacag tgtaggcgaa
    17821 agtttgtaag aattgaatcg ccacagcaat cggagtagag agggcaagag ccgcttctgg
    17881 actgacttta ccagcactag tgatagccat caaggtacca aagataccag gtccgattgg
    17941 gtttggtgga acagtacctc cagcaccaac accgaatccc atataagcca attcaccaat
    18001 agctcccatt gcaagagcag taggcagatc acctagaata attccgacac caaatgacag
    18061 aacaatacat ctattggtgt aaattcctaa cagcattcca ctaaaacaga aagctgtcca
    18121 taatccaatt aaaatcgctt gaaatacatt aatcgacata acgatctcct cttttaaata
    18181 tagtccataa tgttgacttg aacagctcca tcatttcctg ttggagttgt tttcgtatta
    18241 aatgttactt gatgagtttg gcttaattcc ttgagggctg ccttgtcctc ttcacccagg
    18301 aagatggagc gtgtcacttg ctctttacca ggggcattgt gaatgttccc aatattgatt
    18361 tctttgatag ggacaccacc ttctaccaag gttaaagcgt cctttacatc ctttacaacg
    18421 ataaagatcg tttgagcagg attagccttg tgaatgatat caatcacctt ttgcaaaggg
    18481 aagaaacgca tggcaactga gtctggcaca actgttttca tcagagtttg ttgcatcttg
    18541 tccgtgctta cttcgtcatt ggcaacaatg accgtattac aacctaggta ttttacccaa
    18601 agttgtcctt gtccatgaat caaccgttca tcgatacggg tcataataat atttggcatt
    18661 gtcatatttc tcctcctacc aatataggtt ccagtctttg tagaaacgga taagggcttc
    18721 taggtaatag tagtcacccc agatattgcc ttcatccact cctttacctg aatgccatga
    18781 gtacacaccg tggaggagac ttgtcccacc aggggtaaat tgatcatttg cataatgttc
    18841 gatcaaggaa cgaagcatgg catgcatagc atgtttataa atatctttgt cagcatccac
    18901 ctctgggaga tgttttagca tttcatgaat cccacagacg gcgatagctg ttgctgaaga
    18961 atctcgtgat tgatcactac catcattaaa aatcaaatcc caataggaca catgatcttt
    19021 tggcagacga ttcaagaaat aattggtcac acccttaaac aagtcaaagc aggactcgtc
    19081 ttttaagtga cgataagtca aaggaatacc atagactccc catgattgac cacgtgccca
    19141 gcatgaatca tcactatacc cttgtctcgt tacaccttta aagggttgac ctgtctcagg
    19201 atcaaaatag aaggtgtgga aggacgaagc gtcatcacgg attacattat tagctgaagc
    19261 atagaaatgg ctttctgcaa tatcgtagta tttttgatcg cctgtttctt gataagcaaa
    19321 gaataagagt tggatattga gcaagcagtc gataatcaaa cggtaatgct ctttcttgcc
    19381 caagtctccc caagcttgaa taaaaccacc tttttcttga tagcgttcaa tcaacttatc
    19441 tgcagctttc aaggttgctt ctctagtctc tccatctcca tttatcttat attcagccat
    19501 acaagacggt gtgtacaaga agccgagatc atgatgatcc aattctactc tcttattgac
    19561 acgatccagg aaagaaagaa catttttatg agcgatgttt ttaaatgcat cctgttgact
    19621 gtattcataa gccaaccaca gttctcctgt ccagaaacca ttggtccatt ccgtgttatc
    19681 catgattgga tagacattat caaaggtagc tggcgtcggg aaatcttcct tgaaatagtc
    19741 aaggttgagt tctaactgcc gaataacctt atcgattgcc tggccgactt cttctttcgt
    19801 cagaagtggt acttctaaga agcgctcagg cgactttatt ttttcaatcg taaccttttt
    19861 tatcattctt tcacccttta aatacccgat tcgaaatctt cttcctcttc ctctgcatcc
    19921 gttgaaaaca attctttacc atttacgact ccgccctggg ccgccactac tgatttatta
    19981 acaacctcat caaagctatg agccattcta gcaaagactg cttccattaa catggctaag
    20041 ttcaaacccg agagaacatt cattgttttg gctggatttt ctcccattat ggtagaagaa
    20101 accttgaatg gcgatcctcc caagagatca cttaggatta aaacttcttc ttcatttgaa
    20161 attgcaagta agattttttg cttgagttca tctgctgaca ttccttccac aaagtcaatc
    20221 gcctccacat tttcttgatt gcctgcaatc aattgtaaag aactataaat ccctgtagca
    20281 aaatgtccat gccctacaag tacaattttc atcttttctc ctttcttgtt taggtttacc
    20341 ggtttacttt atattcccca ttgtaatcct ttatttttat cttgtcaata gtttttttaa
    20401 aaaaatatta gcgctttcat
//
LOCUS       DBERDK010000035        19734 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.035, whole genome shotgun sequence.
ACCESSION   DBERDK010000035 DBERDK010000000
VERSION     DBERDK010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 19734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19734
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.035"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(166..780)
                     /locus_tag="ACL73L_001233"
     CDS             complement(166..780)
                     /locus_tag="ACL73L_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HGS0772210.1"
                     /translation="MGQTIISAIGVYISTSIDYLIILIILFAQLSQNKQKWHIYAGQY
                     LGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIPIYLGIRFAIVGEDAEEEEEEIIERL
                     EQSKANQLFWTVTLLTIASGGDNLGIYIPYFASLDWSQTLVALLVFVIGIIIFCEISR
                     VLSSIPLIFETIEKYERIIVPLVFILLGLYIMYENGTIETFLIV"
     gene            complement(1596..1865)
                     /gene="rpsO"
                     /locus_tag="ACL73L_001234"
     CDS             complement(1596..1865)
                     /gene="rpsO"
                     /locus_tag="ACL73L_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HGS0772211.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRKNDVNRYRELINSLGLRR"
     gene            complement(2006..2635)
                     /locus_tag="ACL73L_001235"
     CDS             complement(2006..2635)
                     /locus_tag="ACL73L_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HGS0772212.1"
                     /translation="MKLAVIAANGQAGKAIVEEAVKRGHEVTAIVRSENKSQAESIIK
                     KDLFELTKDDLTGFDAVISAFGAYTPDTLPLHSKSIELFNQLLAGTQTRFLVVGGAGS
                     LYIDETKTTRLLDTSDFPEEFKSLAKDQADELDLLRTKNNLNWTFVSPAVDFIPDGEK
                     TGNYILAGEIFTTNEKGISQISYADYAIGLVDELEKGHHIKERISLLEK"
     gene            2874..3248
                     /locus_tag="ACL73L_001236"
                     /pseudo
     CDS             2874..3248
                     /locus_tag="ACL73L_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397621.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3505..3645
                     /locus_tag="ACL73L_001237"
     CDS             3505..3645
                     /locus_tag="ACL73L_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HGS0772213.1"
                     /translation="MELQELVERSWAIRQAYHELEVKHHDFKWTVEEDLLALSNDIGN
                     FQ"
     gene            3652..3828
                     /locus_tag="ACL73L_001238"
     CDS             3652..3828
                     /locus_tag="ACL73L_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HGS0772214.1"
                     /translation="MTKQGRYYDETPYTLEQKLSENIWWLLELSQRLDIDILTEMENF
                     LSDKEKQLNVRTRK"
     gene            3886..5829
                     /gene="thrS"
                     /locus_tag="ACL73L_001239"
     CDS             3886..5829
                     /gene="thrS"
                     /locus_tag="ACL73L_001239"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HGS0772215.1"
                     /translation="MINITFPDGAVREFESGVTTFEIAQSISNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHEDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGF
                     YYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAREIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFDKKDLKNYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRE
                     LERYIVNKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVDYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVDYAWEVA
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(5878..6852)
                     /locus_tag="ACL73L_001240"
     CDS             complement(5878..6852)
                     /locus_tag="ACL73L_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HGS0772216.1"
                     /translation="MLDWKQFFLAYLRSRSRLFIYLLSLAFLVLLFQFLFASLGIYFL
                     YFFFLCCFVTILFFTWDILVETQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLVLKQVQIEDLVKEIIRKYALFFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQELCIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LTQQSSGLGLYLSKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
     gene            complement(6845..7522)
                     /locus_tag="ACL73L_001241"
     CDS             complement(6845..7522)
                     /locus_tag="ACL73L_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGS0772217.1"
                     /translation="MHKILLIEDDQVIRQQVGKMLSEWGFEVVLVEDFMEVLSLFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYAGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDDLMRELWNSDFFIDDNTLSVNVARLRKKLEEQGLVGFIETKKGI
                     GYGLKHA"
     gene            7678..8751
                     /locus_tag="ACL73L_001242"
     CDS             7678..8751
                     /locus_tag="ACL73L_001242"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HGS0772218.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLDLAPQVPRDPTMLTSKNRLQLLDELAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEDFHLTYMKEIPAQKHALRYLKNFFAHHPKQKV
                     ILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIF
                     IPQGSIIGMMLEIPAHQIIVQSTALGGIAQHDTFSWQIEDKHFVQLDKTNSDSQQVDT
                     TFKEWVATVPDEELQLYFDLFFGTILEAGISSINDLASLKALEYIHHLFVQAQSLTPE
                     ERETLGRLTQLLIDTRYQAWKNR"
     gene            complement(8842..9144)
                     /locus_tag="ACL73L_001243"
     CDS             complement(8842..9144)
                     /locus_tag="ACL73L_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772219.1"
                     /translation="MYLVIGIVLAFIVSFWKDNRSLWNPVLFLLSLISSYFYLSYLFY
                     KNGYENVQLAFYVFAFVLLPFLLFLSGIFLIYNGVILLKREGRSKPHYLSMLFDFY"
     gene            complement(9212..9649)
                     /locus_tag="ACL73L_001244"
     CDS             complement(9212..9649)
                     /locus_tag="ACL73L_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HGS0772220.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKESKVTSDFLAASVGVNPVIIRK
                     ILSQLKKAELISVARGTGGTEIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQTSLEKVVADAESQMKD"
     gene            complement(9725..10567)
                     /locus_tag="ACL73L_001245"
     CDS             complement(9725..10567)
                     /locus_tag="ACL73L_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HGS0772221.1"
                     /translation="MTKIALLSDIHGNTTALEAVLADAQQLGVDEYWLLGDILMPGTG
                     RRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEE
                     IEVLHNQPLQIHRQFGDLTVGISHHLPDKNWGRELIHTGKQEEFDRLVTHPPCDIAVY
                     GHIHQQLLRYGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDDKGLVDMDFRR
                     VDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFLRELAQKEGCDRELDDWLK
                     SGND"
     gene            10679..11329
                     /locus_tag="ACL73L_001246"
     CDS             10679..11329
                     /locus_tag="ACL73L_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HGS0772222.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEVLE
                     HTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRVHD
                     NFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNMDIAKQLYVEKIN"
     gene            11450..12157
                     /locus_tag="ACL73L_001247"
     CDS             11450..12157
                     /locus_tag="ACL73L_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0034257 - nicotinamide riboside
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter family
                     protein"
                     /protein_id="HGS0772223.1"
                     /translation="MKKSLKIFATSKWFDLFGVALVVGIAIASGYLNSRLDKFVDWGP
                     WTALVPFGLISVTNVGISMLSTRFTGKLSKWGNYFSIVNTILSGAIDYILGNKAAIIT
                     YPVTFLIYTFAIKKWKASQEGRPNQMSQKQVKLTVIIISIIAFLFAFVTNYIGYEGKM
                     NLLAYVTTIAFALSLIANALNALKLTTQWGFWLIYNFVQLTKAGIQGNFANIGKYIFY
                     ILNAIGALFVWNDEEVR"
     gene            12288..12701
                     /locus_tag="ACL73L_001248"
     CDS             12288..12701
                     /locus_tag="ACL73L_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772224.1"
                     /translation="MKKLYRIHFIAIAVIDLLLFAFFIIRLETSFEWLLLSGLIFFLA
                     QGLLLFLLIVRLKHQFAEIYPQINKKIRFYYLGVLTIDFLFFVLLAFISSQRFSSLMP
                     IITACHSTFYYMTADYLRENYPDFYDKHISLWECL"
     gene            complement(13173..13616)
                     /gene="dtd"
                     /locus_tag="ACL73L_001249"
     CDS             complement(13173..13616)
                     /gene="dtd"
                     /locus_tag="ACL73L_001249"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HGS0772225.1"
                     /translation="MKIIIQRVKKAQVSIEGQIQGKINQGLLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDAEGKMNLSVKDIEGEILSISQFTLFADTKKGNRPAFTGAAKLDMA
                     SDFYDAFNQKLAQEVPVQTGIFGADMQVELVNNGPVTIILDTKKR"
     gene            complement(13646..15868)
                     /locus_tag="ACL73L_001250"
     CDS             complement(13646..15868)
                     /locus_tag="ACL73L_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HGS0772226.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVHFVHKALVYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRVIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTERHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEV
                     AEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMMELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IIANPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYSSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
     gene            16862..17491
                     /locus_tag="ACL73L_001251"
     CDS             16862..17491
                     /locus_tag="ACL73L_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HGS0772227.1"
                     /translation="MKIHKTVNPVAYENTYYLEGEKHLIVVDPGSHWEAIRQTIEKIN
                     KPICAILLTHAHYDHIMSLDLVRETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFRFKVLPTPGHSIGGVSLVFPDAHLVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLPNYDVYPGHGPATTIAHEKAFNPFF"
     gene            complement(17614..19506)
                     /gene="pepO"
                     /locus_tag="ACL73L_001252"
     CDS             complement(17614..19506)
                     /gene="pepO"
                     /locus_tag="ACL73L_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HGS0772228.1"
                     /translation="MTRYQDDFYDAINGEWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVPEDAILENFVKYHRLVRDFDKREADGITPILSLLKEFQELETFADF
                     IAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVAAVVLSNEESSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEEAWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKADVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRPEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
BASE COUNT         5783 a         4463 c         3638 g         5850 t
ORIGIN      
        1 ttcttatttc attttactat atttataaga atctcctaat caaatcaaaa gccactctac
       61 aatagagtaa ctttaagatt gtccatttat actcaatgaa aaagccctct gaaaatcaga
      121 gagctgattt gagctcgggc taaaatccta gtgaaacaaa aaaatctaca cgatcagaaa
      181 agtctctatc gtgccatttt catacatgat gtatagtcca agtagaatga atactaaggg
      241 cacaatgatt cgctcgtatt tttcaattgt ctcgaatatt aacggaatag aggataacac
      301 cctactaatc tcgcaaaaga taattatgcc gattacaaac acaagcaagg ccacgagggt
      361 ctgtgaccaa tctaacgaag caaaataagg tatatagata cctaaattat ctccgccaga
      421 cgcaattgtc agcaatgtaa ctgtccaaaa cagttgattt gccttgcttt gttctaatct
      481 ttcaataatt tcttcctctt cttcttccgc atcttctcca acaattgcaa agcgaatccc
      541 taaatagata gggattaaac caagcaatcc aaccatccat tcttcaggca cgaaattaac
      601 gacataagca gcaactaaac tcgcccctac aagtaagcct gtgcctagat attgccccgc
      661 ataaatatgc catttctgtt tattctgtga tagctgtgca aataaaataa ttaaaataat
      721 taaataatcg atactggtgg aaatataaac accaatagca gatatgattg tctgtcccat
      781 aaaaaaaacc tcctgtatta gtcagtaata aatcagcaga ttttaggaga gcacagacac
      841 attaatttag ctatcgctag tgggtaatgt cgaacgtagg aaagcatttt actcctcctc
      901 aaaacgtagt tacaaagtaa tttctaactg gaattcagtg actacttcca tgtaaagtat
      961 aacatactat actttacttt gtaaagagtg ggctcttcta gggggcttgc atacagctac
     1021 tcggcttgaa aagccgagta actgcgcacc tttatggtgt aattagctga taccgtttga
     1081 ggtttttgta gtctccaaaa ttgttaccga taaaacctca ataaaaaagc cctctgaagt
     1141 cagagagctg atttgagccc gggctaaaat cctagtgaaa aagatgaaac tccttgtgct
     1201 catcgaacac tgtgtcattt ccctattttc atacggattt ttgacgccct tagcatcatg
     1261 attcttgcta gataaatcgt ttatagacta ggcgacgagg cgaagatcgt acgaaacttt
     1321 cagtgaaaca aaaaaaatct ccccgaaggg agaaaagctg gtttatttta ccttacagtg
     1381 ctaggaaccg taaccaaaga ttatacttga gtatatcgag gtaaggtgac aacgcaaaaa
     1441 agctacccaa tttagagtag cttcattatc aagatatgct caattgaaca cggcctaagc
     1501 acttggcaaa aaagataaaa tctcctagaa actgaagttt cttcgtcaat tttcctattt
     1561 ttgctttgtg cttttgacag catttgtatc ttgaattagc gacgaagtcc tagagagttg
     1621 atcaactcac ggtaacggtt aacgtcgttt ttacgcaagt atgcaagcaa gttacgacgg
     1681 cgaccgattt ttttcatcaa tccacggtaa gtagcgtggt cttttttgtg ttgtttgatg
     1741 tgttcgttaa ggtggttgat ttcccaagta aggacagcaa cttgaacctc tactgaacct
     1801 gtatcacctt cgtgacgtgc atattgtgca atgatttcat tttttttctc ttttgagatt
     1861 gccatgatgt ttctcctttt tatttggctt catccgagtg acaggttggc atgcctgtaa
     1921 ccaagaagaa gttatttgtc tttacgacaa tccctatttt accaagattt aaattttctg
     1981 tcaaacatta atactctcat agttattatt tttcaagtaa agagatacgt tcttttatat
     2041 gatgaccttt ttcaagttca tctaccaatc caatagcata atctgcataa ctaatctggc
     2101 ttattccttt ttcattagta gtaaaaattt ctcctgctag aatgtagttt cctgtttttt
     2161 caccatcggg aataaaatct acagcaggac tgacaaaagt ccagttgaga ttattttttg
     2221 tacgaagaag atccaattca tccgcttgat cttttgctag tgacttaaat tcttcaggaa
     2281 aatcagacgt atctaaaagt cgtgtcgttt ttgtttcatc tatataaaga cttcctgctc
     2341 cccctacaac tagaaaacgt gtctgtgttc cagcaagaag ctgattaaat agttcgattg
     2401 atttgctgtg gagcggtagc gtatctggtg tataagcacc aaacgctgaa ataacagcat
     2461 caaatccagt aagatcatct tttgtcaact caaataaatc ttttttaata atagactcag
     2521 cttgactttt gttttcagaa cgaacaatag ccgttacttc atgtcctcgt ttgactgctt
     2581 cttcaacaat tgctttcccc gcttgtccat ttgctgcaat aactgctagt ttcatttttt
     2641 atacctctct tgttgtaatt attttagtta caataattgt gacactctta ataatcaatg
     2701 tcaatagtct tgcttaatta ttatcaaaat atttctacca agaaaactaa ctatgattct
     2761 agtgaaaaga aaaatcttct ttgtcaacaa atttactttc ttgttttaaa catgctataa
     2821 taatcatagc aagagatcta agttgtctgt ttttttaaaa cgaggtgatt atcatgcgta
     2881 gattctattc ccatctcccc tactatctgg tcatattatt cttttattgg ccactttatg
     2941 agttgttctt actagttgtt tctgaccccc ttacactcaa ggaactctat ataaacaatc
     3001 ttctcttctt tacacctctg gtaatcttga ttatatcgtt actctatagc taccgtttcc
     3061 gtttctcact ttgatggtta gttggtaacg gactgctctt ttactttact atcataacct
     3121 ttggtgagtt tatactaatt tacttgctaa tctatgaaac agttgctctg gtcggcatgg
     3181 attctggtat tagcatcaag catattctac aaaaaatgaa aaacaaaaaa ctttcacaaa
     3241 atccttgaaa aatctcacaa tcatgctata ataatccata gagacaagtc acttagtccc
     3301 tttctactag agagtgcgtg gttgctggaa acgcatagga agtctaaact gatactactc
     3361 ttgagttttt tatgaaaaca taaaacggtg gccacgttag agccgatcag aggtgtccct
     3421 ctcttttgag gtacataaat gaaggtggaa ccacgttgcg acgtcctttc gaggatgtcg
     3481 cattttttta ttaggatact aattatggag ttgcaagaat tagtggagcg cagttgggca
     3541 atccgacaag cttatcacga actggaagtt aagcatcatg atttcaagtg gacggtagaa
     3601 gaagacctct tggctttatc taatgatatt ggaaatttcc aatgactggt gatgacaaag
     3661 caaggacgct actatgatga aacaccctac acactggaac aaaaactttc agaaaatatc
     3721 tggtggctat tagaactttc tcaacgtttg gatatagaca ttctgacgga aatggaaaac
     3781 ttcctctctg ataaagaaaa gcaattgaac gttaggactc ggaagtagtc tgctgataaa
     3841 aaatcaatgc ttagaaacta tgaaataata aaaaaggaga acatcatgat taacattact
     3901 ttcccagatg gcgctgttcg tgaattcgaa tctggcgtaa caacttttga aattgcccaa
     3961 tctatcagca attccctagc taaaaaagcc ttggctggta aattcaacgg caaactcatc
     4021 gacactactc gcgctatcac tgaagatgga agcatcgaaa ttgtgacacc tgatcacgaa
     4081 gatgcccttc caatcttgcg tcactcagca gctcacttgt tcgcccaagc agctcgtcgt
     4141 cttttcccag acattcactt gggagttggt ccagccatcg aagatggttt ctactacgat
     4201 actgacaaca cagctggtca aatctctaac gaagaccttc ctcgtatcga agaagaaatg
     4261 caaaaaatcg tcaaagaaaa cttcccatct attcgtgaag aagtgactaa agacgaggca
     4321 cgtgaaatct tcaaaaatga cccttacaag ttggaattga ttgaagaaca ctcagaagac
     4381 gaaggcggtt tgactatcta tcgtcagggt gaatatgtag acctctgccg tggacctcac
     4441 gttccatcaa caggtcgtat ccaaatcttc caccttctcc atgtagctgg tgcgtactgg
     4501 cgtggaaaca gcgacaacgc tatgatgcaa cgtatctacg gtacagcttg gtttgacaag
     4561 aaagacttga aaaactacct tcaaatgcgt gaagaagcta aggaacgtga ccaccgtaaa
     4621 cttggtaaag agcttgacct ctttatgatt tcacaagaag tgggacaagg tttgccattc
     4681 tggttgccaa atggtgcgac tatccgtcgt gaattggaac gctacatcgt aaacaaagag
     4741 ttggcttctg gctaccaaca cgtctacact ccaccacttg cttctgttga gctttacaag
     4801 acttctggtc actgggatca ttaccaagaa gacatgttcc caaccatgga catgggtgac
     4861 ggggaagaat ttgtccttcg tccaatgaac tgtccgcacc acatccaagt ttttaaacac
     4921 catgttcact cttaccgtga attgccaatc cgtatcgctg aaatcggtat gatgcaccgt
     4981 tacgaaaaat ctggtgccct cactggcctt caacgtgtac gtgaaatgtc actcaacgac
     5041 ggtcacctat tcgttactcc agaacaaatc caagaagaat tccaacgtgc ccttcagttg
     5101 attatcgatg tttatgaaga cttcaacttg actgactacc gcttccgcct ctctcttcgt
     5161 gaccctcaag atactcataa gtactttgat aacgatgaga tgtgggaaaa tgcccaaact
     5221 atgcttcgtg cagctcttga tgaaatgggc gtggactact ttgaagccga aggtgaagca
     5281 gccttctacg gaccaaaatt ggatatccag attaaaactg cccttggaaa agaagaaacc
     5341 ctttctacta tccaacttga tttcttgttg ccagaacgct tcgacctcaa atacatcgga
     5401 gctgatggcg aagatcaccg tccagtcatg atccaccgtg gggttatctc aactatggaa
     5461 cgcttcacag ctatcttgat tgagaactac aagggggcct tcccaacatg gctggcacca
     5521 caccaagtaa ccctcatccc agtatctaac gaaaaacacg tggactacgc ttgggaagtg
     5581 gccaagaaac tccgtgaccg cggtgtccgt gcagacgtag atgagcgcaa tgaaaaaatg
     5641 cagttcaaga tccgtgcttc acaaaccagc aagattcctt accaattaat tgttggagac
     5701 aaagaaatgg aagacgaaac agtcaacgtt cgtcgctacg gccaaaaaga aacacaaact
     5761 gtctcagttg ataattttgt tcaagctatc ctagctgata tcgccaacaa atcacgcgtt
     5821 gagaaataag tgtctagcat aaaagcctcc aatctggagg ctttttctca tctattttta
     5881 ctcaaggact aagttcactt gagcaaactg aatccgcact gtcgttcctt ttccgacctc
     5941 agactcgata cgaatctggt gccccagttc ttcagaaatt ttcttagata gataaaggcc
     6001 aagtccagag gactgctggg tcaaacggcc attgtatcct gaaaagccac gttcaaatac
     6061 tcggaggaca tcactgtttt ttatcccgat tcccgtatct ttgatacaaa gctcttggtc
     6121 atccatataa atctccagac caccttcctt ggtgtacttg agactgtttg agatgatttg
     6181 ctcaataacc actagcagcc actttttatc cgtcacgatt tctttatcaa ggtcatgtag
     6241 attgacattt aagccttttt gaataaagaa aagagcatat ttacgaatta tttccttgac
     6301 caagtcctca atttgaacct gctttaagac caaatcatca tggaaacttt ctaaacgcag
     6361 gtactgtaaa actaggttgg tataggagtc gattttgaaa atttcctgtt ctagctgctg
     6421 cttcagttgg cggtcgacca cttctgcaac taagagttga ctggctgcaa tgggggtctt
     6481 tatctgatgg acccacaagg tatagtaatc cagcaaatcc gtcagttttc tttctgcttt
     6541 tgacctctgc tgatagagtt ccatctcacg cgcttctaat ttttctgcta aagctatttc
     6601 caaaggagac ttggcttccc tctctccata gagaagttcc tggcgataga cctgcgtttc
     6661 caccaatatg tcccaagtga aaaataagat ggttacaaag caacacaaga agaaaaagta
     6721 gaggaagtaa attcctagac tggcaaataa aaactgaaag agtaagacaa gaaatgccaa
     6781 agaaagcaga tagataaaaa gacgactacg ggagcgcaga taggctagaa aaaattgttt
     6841 ccaatcaagc atgcttcaat ccgtacccta ttcctttctt ggtctcgata aatcctacca
     6901 atccctgctc ctccaacttt ttacgcaaac gagccacatt gacagagagg gtattatcat
     6961 caatgaaaaa gtcactgttc caaagttccc gcatcaggtc gtcacgtgct acgatgttgc
     7021 ctgcatgctc aaataacacg cgtaaaatct ggaattcatt cttggtcaaa ttcaagactt
     7081 gcccttgata atgtaaatcc atggatttgg tattgaggat aacaccagca tattccagca
     7141 aactctcatc acgcccaaac tcataggaac gacgcaacaa gccctgaacc ttagctaaaa
     7201 gaacctgctg gtcaaaaggc ttggtcacaa agtcatccgc ccccatattg attgccatga
     7261 caatatccat agcctggtct ctcgaagaaa gaaacatgat aggtaccttg gaaatcttgc
     7321 ggatttcctg acaccagtga taaccattaa acaagggcaa accaatatcc atgaggacca
     7381 gatgaggttc cgactgaaca aatagactca aaacttccat aaagtcttct accaggacca
     7441 cttcaaatcc ccattcagag agcattttcc cgacctgttg acgaatgacc tgatcatctt
     7501 ctattaataa aatcttgtgc atgcgcttct ccttttctat tattataaca gatttttcca
     7561 tgccagatgg tctgaaactg aatttgaaat agcctgtttt tagccagtac aaacaggcaa
     7621 tgctactagc taatttgagg gaaatttgct aagataaata aaaagaaagg agctcttatg
     7681 gccaatattt ttgactatct gaaagatgtc gcatatgatt cttattacga ccttcccttg
     7741 aatgagttag acattctaac cttaatagaa atcacctacc tctcctttga taatctggtc
     7801 tccacacttc ctcaacgtct tttagatcta gcacctcagg ttccaagaga tcccaccatg
     7861 cttactagca aaaatcgcct tcaattatta gatgaattgg ctcaacacaa gcgcttcaaa
     7921 aattgcaaac tctcccattt tatcaacgac atcgaccctg aactgcaaaa gcaatttgcg
     7981 gctatgactt atcgtgtcag cctcgatacc tatctgattg tctttcgtgg gacagatgac
     8041 agtatcattg gctggaagga agatttccac ctgacctata tgaaggaaat tcctgctcaa
     8101 aagcacgccc ttcgctattt aaagaacttt tttgcccatc atcctaagca aaaggttatt
     8161 ctagctgggc attccaaggg aggaaatctc gctatctatg ctgctagcca aattgagcaa
     8221 agtttgcaaa atcagatcac agcagtttat acatttgatg cacctggtct ccatcaagaa
     8281 ttgacacaga ctgcgggtta tcaaaggata atggatagaa gcaagatatt cattccacaa
     8341 ggttccatta tcggtatgat gctggaaatt cctgctcacc aaatcatcgt tcagagtact
     8401 gccctgggtg gcatcgccca gcacgatacc tttagttggc agattgagga caagcacttc
     8461 gtccaactgg ataagaccaa cagtgatagc cagcaagtag acacaacctt taaagaatgg
     8521 gtggccacag tccctgacga agaacttcag ctctacttcg acctcttctt tggcactatt
     8581 cttgaggctg gtattagctc tatcaatgac ttggcttcct taaaggcgct tgaatacatt
     8641 catcatctct ttgtccaagc tcaatccctc actccagaag aaagagaaac cttgggtcgc
     8701 cttacccagt tattgattga tactcgttac caggcatgga aaaatagata atactcttga
     8761 caattaaatg tatacaaaac aaaagaccta gaatacatac tttcatgtgc attctaagtc
     8821 tttttaaata gaatctaata gtcaataaaa atcaaagagc attgagagat aatggggctt
     8881 ggaacgtccc tctcgcttca acaaaatgac cccattatag attaaaaaga tgccacttag
     8941 aaaaagcaaa aaaggaagta agacaaaggc aaatacataa aaagctaact gaacattctc
     9001 gtatccattt ttataaaaaa ggtaggatag ataaaaataa cttgaaatga gggataataa
     9061 aaataatact ggattccaca aacttctatt atccttccaa aatgacacta taaaggctaa
     9121 tacaattcct ataacgagat acatttctta ctcctttaat agctacattt tatcataatt
     9181 atccaaagaa aaaagagggc atttatccct cttaatcctt catctgactc tctgcatcgg
     9241 ccacgacttt ttctagactg gtttgaccaa gttctgcctc catagtcaac tgaattctct
     9301 ccaatttttg atccaaaaca tcatgaatat gagctcctac agggcaattt ggattcggat
     9361 tgtcatggaa actgaagagt tgacctgtct taccaagaca ttcgaccgcc tgataaacat
     9421 ctaaaagact aatatcctta aggtccttga caatctctgt tccgcccgtt ccacgcgcta
     9481 ctgaaatcag ctctgccttc ttcaactggg acaagatctt tctgataatg acaggattga
     9541 ccccgacact agcagccaga aaatcactgg tcaccttgct ttccttcccc tcgagggcaa
     9601 tgattatcag catatgagtc gcaatggtaa atctacttgg aatttgcatc ctcttctcct
     9661 ttttacgagg ctaccctgcc tctactcttc tttttctatt attataccct ttttagttgt
     9721 aatgtcaatc gttaccactt ttcaaccagt cgtctaactc ccgatcgcag ccctctttct
     9781 gagccaattc tctcaaaaat tcctgatgat gagtatggtg gatcccattg accagacttt
     9841 catagtaaac ctcaaaatag ggaagtctca ggtctttagc cagctgcaat tcagctgcca
     9901 catcgtaatc tacccgtcgg aagtccatat ctaccaggcc cttgtcatca aactccaaaa
     9961 tcatatactg ggcccgcaag tccttccgta gctgagcgtc caaaaagaaa ggttggccaa
    10021 tcgaacccgg attgacaatc aattgcccac cagtcccgta acgaagcaac tgctggtgaa
    10081 tatgtccata aacagcaata tcacagggag gatgagtcac caagcggtca aactcctctt
    10141 gtttgccagt atgaatcaac tctcgccccc agttcttatc aggcagatga tggctaattc
    10201 ccaccgtcaa atccccaaac tgacgatgaa tttgaagagg ttgattgtgg agcacttcaa
    10261 tttcttctag ggaaatttcc tctaaaacat actggcactg gcgcaagaga tagcgttgac
    10321 tggggcgagt actgtccaat tccttacgga caccatgcca aagactgtct tcccagtttc
    10381 ccaaaactct agccgtaatc ggtagttgat ccaacaagtc caaaatcctt ctacgtcctg
    10441 tccctggcat gagaatatct cccaaaagcc agtattcatc cactcctagc tgctgagcat
    10501 ctgccaaaac agcctccaag gcggtggtat ttccatgaat atctgaaaga agagctattt
    10561 tcgtcatatc catctcctcg ttttttctct tgcaataagt ataacataaa aagtcacagc
    10621 tagagaaatc tagctttttt tgatatacta gataaagata ttagacaaga ggaaacgaat
    10681 gaccccaaac aaagaagact atctaaaatg tatttatgaa attggcatag acctgcataa
    10741 gattaccaac aaggaaattg cggctcgcat gcaagtctct ccccctgccg taactgaaat
    10801 gatcaaacga atgaaaagtg aaaatctcat cctaaaggac aaggaatgtg gctatctact
    10861 gactgacctc ggtctcaaac tggtctctga gctctatcgt aagcaccgct tgattgaagt
    10921 ttttctagtt catcatttag actatacaag tgaccagatt cacgaggaag ctgaggtctt
    10981 ggaacacact gtctctgacc tgttcgtgga aagactagat aaactgctag gtttccctaa
    11041 aacctgcccc cacggaggaa ctattcctgc caagggagaa ctcctcgttg aaatcaataa
    11101 cctcccacta gctgatatca aggaagctgg cgcctaccgc ctgactcggg tgcacgataa
    11161 ttttgacatt ctccattatc tggacaagca ctcacttcac atcggtgacc agctccaagt
    11221 caagcagttt gatggcttca gcaatacctt cactatcctc agtaacgacg aggatttaca
    11281 agtgaatatg gacattgcaa aacaactcta tgtcgagaaa atcaactaat ttctcaagtc
    11341 ccctaccaac cctgaaagtt ttattttcag ggttttattt ctatcatttt attttacttt
    11401 catttggtgg tagaatattt gaaaaaaaaa aagaaaggta gtttaaccta tgaaaaaatc
    11461 actaaaaatt tttgctacat ctaaatggtt tgacctcttc ggggttgctt tggtcgttgg
    11521 gattgcgatt gcatctggtt acctcaactc acgcctcgat aaatttgtag actggggacc
    11581 atggacagct cttgtaccct ttggattgat ttccgtaacc aacgttggga tttccatgtt
    11641 gtccactcgt ttcacgggga aattaagcaa atggggaaat tactttagta ttgttaatac
    11701 cattttgtcc ggtgctattg attatatcct tggaaataag gcggccatca ttacctatcc
    11761 cgtcaccttc ctcatttata cctttgctat aaagaaatgg aaagcttcgc aagaaggcag
    11821 acccaaccaa atgagccaaa aacaggtaaa attgacggtc atcatcattt ccatcatcgc
    11881 cttcctcttt gcctttgtga ccaactatat cggatatgag ggcaagatga atctccttgc
    11941 ctacgtaaca actattgcct ttgcactatc cctcattgcc aatgctttga atgcattaaa
    12001 actaacaact cagtggggct tttggttgat ttacaatttc gttcagctga caaaagctgg
    12061 tattcaagga aacttcgcca atatcggtaa atacatcttt tatatcctca atgcaatcgg
    12121 agctttattt gtctggaatg atgaagaagt tagataatag aaggaaactc aaataaagaa
    12181 tactagccaa cttaagagaa tctcccttct gctattttta tcattttagt tgtaactccc
    12241 attcttttgg agtagaatga agttagataa aagagggagg tcttgtcatg aaaaaactct
    12301 atagaatcca ttttatagca attgcagtta ttgatttgct actatttgca ttttttatca
    12361 ttagactcga gacatctttt gagtggctct tgctctctgg tttaattttc ttccttgctc
    12421 aaggactcct gctatttctc ttgatcgtcc gactcaaaca tcaattcgct gagatttatc
    12481 ctcaaatcaa taaaaagatt cgcttctact atttaggggt tctcaccatt gattttctat
    12541 tttttgttct cttagccttc attagttctc agcgtttttc atctcttatg ccaatcatca
    12601 ctgcttgcca ttctactttt tattatatga cagctgacta cctaagagaa aactatccag
    12661 acttttacga caaacacatc tctttatggg agtgtctcta aggaaaagga ggttttagca
    12721 tgaaaaaaat catcttcatc aaaaccattc aactccttgt cattgatgga atcatgctag
    12781 catttttgac atttaaaagg ggggcttact tgggactgga ttttgattta tagcggttgg
    12841 ctcattttct ttcatcctgt gctattgacc tatctttcaa accaactttg tgaccacttt
    12901 agttaactct attcccagat tagaccgaga ttctggcgtt ttgctttaca aattctccta
    12961 tgggatagcc tgatgattct ctccttggtg tctttaagtg atattccact tttccttcag
    13021 ggaactctcc tcatcctagg acatctcatc ccttcctatc gcatctgcca aagcctgaaa
    13081 agagacttcc cccaagcata tcaagaaccg atttcttttt ggagtatttt atgatagatg
    13141 agaaagacca agccgactgg gcttggtctt tcttatctct ttttagtatc taggataatg
    13201 gtaacaggtc cgttattaac cagctcaacc tgcatatctg ctccaaagat gcctgtctga
    13261 acgggcactt cttgcgctaa tttttgattg aaagcatcat agaagtctga tgccatatca
    13321 agtttagctg cccctgtaaa ggctggacga ttgcctttct tagtatccgc aaagagggta
    13381 aactgagaaa tagagaggat ttctccttca atatctttga cagacaggtt catcttgcct
    13441 tctgcgtctg aaaaaatccg catattgacc agttttctca cagcatagtc caaatcttcc
    13501 tcttggtcct ctggtccaac accaaccagc aataaaagtc cctgattgat ttttccctga
    13561 atctggcctt ctatactcac ttgggctttt ttaacccgtt ggataatgat tttcataata
    13621 gcctttctag taagagctag gacaactagc cgttggtccg tttgacagag taaacttctg
    13681 gcacactctt aattttatcg acaaccgtgg tcagtgtaga gaggttggca ataccgaagg
    13741 acacatggat attagcaaac ttcatatcct tggttggttg ggcattgacc gttgaaatat
    13801 tcttggttgt atttgaaaga acttgcagta catcgttcaa cagtcctgta cggttgagac
    13861 cgtagatatc gatatgggcc atatactcct tatttgagct agagtactgg tcttcccatt
    13921 ccacatcaag gagacgttgc tcgtagtttt cttgggcacg caggttcata cagtccacac
    13981 ggtgaatagc cacaccacga cccttggtaa tgtagccaac aatatcgtca ccaggcacgg
    14041 ggttacaaca cttagcaatc cgcactagga gaccagaagc accttcaata accactcccc
    14101 cctcatgctt gaccttgaga gtttctttat tttcaacctt gacctcgcca cctttgacaa
    14161 gctcctctgc ctcagccttg gccttggcac gctcttcctc acggcgttcc ttttcagtca
    14221 gacggttaaa gacggtaatc gcaccgattt ccccaaaacc aatggccgca aagagggagt
    14281 cttctgtctt gtaactggtc ttttgcagaa cttgatccat gtggcgcttg tccataaatt
    14341 tatttgccac atagccattt tcttggaact gagccatcag catctcacga cccttgttga
    14401 cagacaattc cttatcttgg tttttaaaga actggcgaat cttattgcgc gccttgctag
    14461 tcttgaccat attgagccag tcacggctag gtccaaagga gttcgggttg gcgataattt
    14521 caacctgatc ccctgtcttt aacttggttg tcagtggaac catgcggcca ttgaccttgg
    14581 caccagttgc tttttcaccg accttggtat ggatttcgta ggcaaaatca atcggtcctg
    14641 aatctttggg aagggaacgg acagctccat ctggggtaaa aacgtaaatc tcctcagcca
    14701 aatagttttc cttaacagag tccacaaatt ccttagcatc atcagcctgg tcttggagct
    14761 ccatcatctc cttgatccag ttcattccaa tagctgattc cttgctgtta acttgcccct
    14821 ttataccttt cttataagcc cagtgagccg caaccccgta ctcagccacc tcgtgcattt
    14881 ccttggttcg aatctggaat tcaatcggcc cttttggtcc ataaacagtc gtatggatag
    14941 actgataacc attggccttg cggttggcaa tatagtcttt gaagcgacct ggcatcggtt
    15001 tccaaaattc atgcacgtaa ccaagcatgg cataaacatc actttgggta tctaaaatac
    15061 aacgaatagc aatcagatca tagatttcct caaaccgttt tctcttgtcc tgcattttgc
    15121 ggaaaattga gtaaatatgc ttgggacgac cataaatctt ccctttcaag tgacgttctg
    15181 tcgtgtactc ctctaatttt gtgactacct catccaccaa ggcctcacgc tccctgcgct
    15241 tttccttcat catatgggta atcttgtaaa actccgttgg attgagataa cggaaagaca
    15301 agtcttctaa ttcccatttg acactggaaa tccccaaacg atgggcaagt ggggcataga
    15361 tttccatggt ttctttggaa atacgctcct gcttgtcttt tcgaagatgt ttcagggtcc
    15421 gcatattgtg caagcggtca gacagtttga ccaaaataac gcggatgtcc tcagacatgg
    15481 ccatgagcat cttgcgatga ttttccgcta attgctcctc gatcgatttg tactcgacct
    15541 tgccaagctt ggtaactccg tcaacaatca cccgcacatc aggaccaaac tctctttcca
    15601 aatcgtccaa ggtcgcatct gtatcttcca ccacatcatg caagaatcca caagctactg
    15661 ttacagcatc cagctttagc ttagctaaaa tacctgccac ttggataggg tgaatgatat
    15721 aaggctcgcc tgatttgcga tattgaccac tgtggcattc aacagcatag accaaggcct
    15781 tatggacaaa atgaacatcc tcttccgtta aatattcttt ggttaaagcg acaacttctt
    15841 cgcctgttaa attcacttct ttcggcatct ctactctcca attcttccta ccattttatc
    15901 acttttttaa gaatatgaaa actagattgg aacagaataa gaaaaaaata attcaaaatt
    15961 gcttgataat tctgaattat tggcccataa tatactacga agttagattt taaacttagg
    16021 tgatagaagg agagatagaa gaacggaaac catattgtaa cccaaagact ttctgatttc
    16081 cccaattcca ttgaagatac gaaagataaa cggtggaact cgtatcacat acactggtac
    16141 cttgactgga ttttggaatt aatactcaat gaaaatcaaa gagcaaacta ggaaactagc
    16201 cgcaggttgc tcaaagcact gctttgaggt tgtagataag actgacaaag tcagtcacat
    16261 atatacggca aggcgacgtt gacgcggttt gaagagattt ttgaagagta taaaaatcct
    16321 caagatactt tcttctatcc tttagtttat aaggagaata cctatgaaaa aaactgctat
    16381 ttctatcttt gctctcctaa tgttaggagt ttgctgcctg ttcctattca gccagcaaag
    16441 ctataaaaaa ctcgttcaat actatgctaa cgaccagaac ctgcccagta ggataactta
    16501 tagtgaatat agcgacaaat gagaagccaa ctacggtagc actctaaaca tcacgtctat
    16561 caaacaagct aatgacggag tttatgcaac ctatgaaggg caattgacac ctttccaata
    16621 ttgataaatt gataaccagc ctgtcttcat ctagtcatgc tggtttttaa gttcatttta
    16681 aatccttacc tattctccct aactgtgcta tacttaattt atactcaatg aaaatcaaag
    16741 agcaaactag aaagctagcc gcaggctgtt caaagcactg ctttgaggtt gcagataaag
    16801 ttgacgcggt ttgaagagat tttcgaagag tattagtaca ttctttgaga ttggagctag
    16861 tatgaaaatc cataaaaccg tgaatcctgt tgcctatgaa aatacctatt atctagaagg
    16921 cgaaaagcac ctcatcgtcg tcgatcctgg tagtcattgg gaagccattc gtcagacaat
    16981 cgagaagatc aacaaaccga tctgtgctat tctcttgacc cacgcccatt atgaccatat
    17041 catgagtctg gacttggttc gcgagacgtt tggcaatcct cctgtctata tcgcagagag
    17101 cgaagccagc tggctctaca ctcctgtcga taatctctcc ggtctccctc gccacgatga
    17161 tatggcagat gtggtcacaa aacctgcaga acacaccttt gtctttcacg aagaatacca
    17221 actagaggaa tttcgtttta aggttctacc gaccccaggg cactctatcg gtggtgtttc
    17281 cctagtcttt cctgatgctc acctagtctt gacgggagat gctctattcc gcgaaactat
    17341 cggacggacc gaccttccga ctggtagcat ggagcaactc cttcatagta tccagaccca
    17401 actcttcacc ctaccaaact acgatgtcta tccaggacat ggtccagcta ctactatcgc
    17461 tcacgaaaag gccttcaatc cctttttcta gcaagatgat gacaatcgaa atttaagtaa
    17521 actatccagc aaatctttct attacaaaag gcatcctatc aaggttttca cacgtgattg
    17581 gatgcctttt ttctgatgac tagatttttt gtattaccaa ataatcacgc gctcctctgg
    17641 tgaacgccac attccgtctc cttctttgac atcataggtt gtaaagaaat cgtcgaagtt
    17701 tggtacttgc acattgacac ggagtttggc tggtgcgtgc acatcgacgc tagccaaaag
    17761 tttcataaat tctggtcgac ctttcatgcg ccagatgcga ccgaagttgt agaagaactc
    17821 ttctgctgag aagtctgctt ctctcttagc tgcttcaagc gctgctgcga ttcctcccaa
    17881 gtcagccacg ttttctgata cagtcaattt accgttaatg gttgctccat aagaatcctg
    17941 tccatcaaat tggtcaatga ctttttgtgt tttctccttg aaggcagcat agtcgctctc
    18001 tgtccaccaa tccttgaggc taccattttc gtcaaaggaa gccccgttag tatcaaaggc
    18061 gtgggaaatt tcatgggcaa tcactgcccc aataccaccg tagttagcag aagatgactg
    18121 atgcaagtca tagaaaggcg cctgtaaaat ggccgctgga aagacaatca ggttcttctg
    18181 aggattgtag taggcattga ccatatgagc aggcatgccc cattccttat aatctacagg
    18241 ctggttccac ttactccaac tgtgcttgat ttccacacgc gcaaaggcta gagcattctc
    18301 aaaaagactg gcagtttcat tcactacctt atccttgtaa cgtgcaggca attcttctgg
    18361 atagccaata taaggtttga tcacattgag cttcacgata gcctgtttac aggtttctgg
    18421 agtgagccag tcattcttaa gcagacgctc cttatagaca tcaatcatgg ttgccacttt
    18481 tttctccaca tccgccttgg cttctggaga gaacttctca cgggcgtacc aaagacccag
    18541 ggcttgcttg aaaggttctt gtgctagatg ataagctgct ttgaccttat cttttgcctc
    18601 tggaactcca gaaagggcac ggctgtaggc accagacaaa acacggatat cctctgttaa
    18661 atagctggtt gaaagattga caacactcaa aatcaaggtt gctttaagga gagaccaggc
    18721 ttcctcactg tagaattgct ctgctgcttg ccagaaacgt tcctcgtcta caataacctt
    18781 gtctggtaat tgcccaataa ctgctttgaa gaagtcatcc aaaggtaggg caggcgcgaa
    18841 tttcttgaaa tcttcgtaag aatatggatg atagagttta gcatattctg aactttcttc
    18901 attagagagc accactgccg caactcggcg gtccaattca agtctttttt ctagcaagtc
    18961 ttcaatttct tcatcagaga aatcataagc cttgaggaga tttgcgctgc tttctttcca
    19021 aagagtcaag agctcttcgc gctgaggatg ttcttctgca tagtaggtcg tatctggcaa
    19081 gattgtgctt ggagcgctag cccatagaac attgattcta gcatccataa agtctggcga
    19141 tacaccaaaa ggaaggaagt ttggttttcc tgcaagctca aactctgcta gtttagctat
    19201 aaaatccgca aaagtctcca attcttgaaa ttctttaagg agtgataaga taggtgtgat
    19261 accgtcagct tctctcttgt caaaatcacg aactaggcgg tggtatttga caaagttttc
    19321 caagatagca tcctcaggca cttcttcacc tgctaaccac ttgtctgttg tcgccagcat
    19381 caagtcttca atttcctggt ctaaatcaac aaaacctcct gtttgagact tatctgctgg
    19441 gatttcagct gtctgttgcc attctccatt gatagcatca taaaaatcat cttgataacg
    19501 tgtcatcttg ttctcgcttt catttgtatt tgcctttatc ttaacaaaaa tcactaggga
    19561 atgcaattag taaccattta actattattt tttagctatt ataaagaatc tttccatgat
    19621 aaaacgcata attccaagtt ttttacactt gatactatgc atactatgcg ttttataatt
    19681 tagaaattta tcctgaaagt tatctttaga atctattttt cagttattat ttta
//
LOCUS       DBERDK010000036        19263 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.036, whole genome shotgun sequence.
ACCESSION   DBERDK010000036 DBERDK010000000
VERSION     DBERDK010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 19263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19263
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.036"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            76..5379
                     /locus_tag="ACL73L_001253"
     CDS             76..5379
                     /locus_tag="ACL73L_001253"
                     /EC_number="3.2.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033926 -
                     endo-alpha-N-acetylgalactosaminidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpGH101 family
                     endo-alpha-N-acetylgalactosaminidase"
                     /protein_id="HGS0772229.1"
                     /translation="MNKGLFEKRCKYSIRKFSLGVASVMIGAAFFGTSPVLADSVQSG
                     STANLPADLATALATAKENDGRDFEAPKVGEDQGSPEVTDGPKTEEELLALEKEKPAE
                     EKPKEDKPVAAKPETPKTVTPEWQTVEKKEQQGTVAIREEKGVRYNQLSSTAQNDNAG
                     KPALFEKKGLTVDANGNATVDLTFKEDSEKGKSRFGVFLKFKDTNNNVFVGYDKDGWF
                     WEYKSPTTSTWYRGSRVAAPETGSTNRLSITLKSDGQLNASNNDVNLFDTVTLPAAVN
                     DHLKNEKKILLKAGSYDDERTVVSVKTDNQEGVKTEDTPAEKETGPVVDDSKVTYDTI
                     QSKVLKAVIDQAFPRVKEYSLNGHTLPGQVQQFNQVFINNHRITPEVTYKKINETTAE
                     YLMKLRDDAHLINAEMTVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDERKLLSSISF
                     LGNALVSVSSDQTGAKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVSTDKLA
                     AGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFPEYTKE
                     LPSAKVVITEDANADKKVDWQDGAIAYRSIMNNPQGWEKVKDITAYRIAMNFGSQAQN
                     PFLMTLDGIKKINLHTDGLGQGVLLKGYGSEGHDSGHLNYADIGKRIGGVEDFKTLIE
                     KAKKYGAHLGIHVNASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDAAYDLA
                     HGRLARWEDLKKKLGDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRFAIEWG
                     HGGEYDSTFHHWAADLTYGGYTNKGINSAITRFIRNHQKDAWVGDYRSYGGAANYPLL
                     GGYSMKDFEGWQGRSDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDNGSTYK
                     WTPEMRVELVDADNNKVVVTRKSNDVNSPQYRERTVTLNGRVIQDGSAYLTPWNWDAN
                     GKKLSTEKEKMYYFNTQAGTTTWTLPSDWAKSKVYLYKLTDQGKTEEQELTVKDGKIT
                     LDLLANQPYVLYRSKQTNPEMSWSEGMHIYDQGFNSGTLKHWTISGDASKAEIVKSQG
                     ANDMLRIQGNKEKVSLTQKLTGLKPNTKYAVYVGVDNRSNAKASITVNTGEKEVTTYT
                     NKSLALNYVKAYAHNTRRNNATVDDTSYFQNMYAFFTTGSDVSNVTLTLSREAGDEAT
                     YFDEIRTFENNSSMYGDKHDTGKGTFKQDFENVAQGIFPFVVGGVEGVEDNRTHLSEK
                     HDPYTQRGWNGKKVDDVIEGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYRVTFEY
                     EAGSDNTYAFVVGKGEFQSGRRGTQASNLEMHELPNTWTDSKKAKKVTFLVTGAETGD
                     TWVGIYSTGNASNTRSDSGGNANFRGYNDFMMDNLQIEEITLTGKMLTENALKNYLPT
                     VAMTNYTKESMDALKEAVFNLSQADDDISVEEARAEIAKIEALKNALVQKKTALVADD
                     FASLTAPAQAQEGLANAFDGNLSSLWHTSWGGGDVGKPATMVLKEPTEITGLRYVPRG
                     SGSNGNLRDVKLVVTDESGKEHTFTATDWPDNNKSKDIDFGKTIKAKKIVLTGTKTYG
                     DGGDKYQSAAELIFTRPQVAETPLDLSGYEAALAKAQKLTDKDNQEEVASVQASMKYA
                     TDNHLLTERMVEYFADYLNQLKDSATKPDAPTVEKPEFKLSSLASDQGKTPDYKQEIA
                     RPETPEQILPATGESQSDTALFLAGVSLALSALFVVKTKKD"
     gene            complement(5546..7705)
                     /gene="pbp1a"
                     /locus_tag="ACL73L_001254"
     CDS             complement(5546..7705)
                     /gene="pbp1a"
                     /locus_tag="ACL73L_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP1A"
                     /protein_id="HGS0772230.1"
                     /translation="MNKPTILRLIKYLSISFLSLVIAAIVLGGGVFFYYVSKAPSLSE
                     SKLVATTSSKIYDNKNQLIADLGSERRVNAQANDIPTDLVKAIVSIEDHRFFDHRGID
                     TIRILGAFLRNLQSNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAIQLEQK
                     ATKQEILTYYINKVYMSNGNYGMQTAAQNYYGKDLNNLSLPQLALLAGMPQAPNQYDP
                     YSHPEAAQDRRNLVLSEMKNQGYISAEQYEKAVNTPITDGLQSLKSASNYPAYMDNYL
                     KEVINQVEEETGYNLLTTGMDVYTNVDQEAQKHLWDIYNTDEYVAYPDDELQVASTIV
                     DVSNGKVIAQLGARHQSSNVSFGINQAVETNRDWGSTMKPITDYAPALEYGVYDSTAT
                     IVHDEPYNYPGTNTPVYNWDRGYFGNITLQYALQQSRNVPAVETLNKVGLNRAKTFLN
                     GLGIDYPSIHYSNAISSNTTESDKKYGASSEKMAAAYAAFANGGTYYKPMYIHKVVFS
                     DGSEKEFSNVGTRAMKETTAYMMTDMMKTVLTYGTGRNAYLAWLPQAGKTGTSNYTDE
                     EIENHIKTSQFVAPDELFAGYTRKYSMAVWTGYSNRLTPLVGNGLTVAAKVYRSMMTY
                     LSEGSNPEDWNIPEGLYRNGEFVFKNGARSTWNSPAPQQPPSTESSSSSSDSSTSQSS
                     STTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP"
     gene            complement(7702..8298)
                     /gene="recU"
                     /locus_tag="ACL73L_001255"
     CDS             complement(7702..8298)
                     /gene="recU"
                     /locus_tag="ACL73L_001255"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HGS0772231.1"
                     /translation="MVNYPHKVSSQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLAVIHKKPTPIQIVQVDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEVKETKQ
                     KRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKGQK
                     SMPLEYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            8364..8891
                     /locus_tag="ACL73L_001256"
     CDS             8364..8891
                     /locus_tag="ACL73L_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HGS0772232.1"
                     /translation="MATALVLGYSAFDLGLFSDKDPRLKLIKKAIRKDLEAMAADGVS
                     WLVFTGSLGFEYWVLEVAQEMKTEYGFQLATIFAFETHGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYQQFLLENTTSSYLFYDEENETKLAYFYQKMKNQEDYFIKRL
                     TFDQLNELAENFSEN"
     gene            8961..9290
                     /gene="gpsB"
                     /locus_tag="ACL73L_001257"
     CDS             8961..9290
                     /gene="gpsB"
                     /locus_tag="ACL73L_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HGS0772233.1"
                     /translation="MASIIFSAKDIFEQEFGREVRGYNKVEVDEFLDDVIKDYETYAA
                     LVKSLRQEIADLKEELTRKPKPSPVQAEPLEAAITSSMTNFDILKRLNRLEKEVFGKQ
                     ILDNSDF"
     gene            9311..9691
                     /gene="rnpB"
                     /locus_tag="ACL73L_001258"
     ncRNA           9311..9691
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACL73L_001258"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            9776..10933
                     /locus_tag="ACL73L_001259"
     CDS             9776..10933
                     /locus_tag="ACL73L_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HGS0772234.1"
                     /translation="MKKEFNLIATAAAGLEAVVGREVRELGYDCQIENGRVRFQGDVR
                     AIIETNLWLRAADRIKIIVGTFPAKTFEELFQGVFALDWENYLPLGARFPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAVMIARK
                     MAPGLRRSFAFEEWNWISDRLIQEVRTEAAKKVDRELELDIMGCDIDARMVEIAKANA
                     QAAGVAGDITFKQMRVQDLRSDKINGVIISNPPYGERLSDDAGVTKLYAEMGQVFAPL
                     KTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            10946..12316
                     /gene="mapZ"
                     /locus_tag="ACL73L_001260"
     CDS             10946..12316
                     /gene="mapZ"
                     /locus_tag="ACL73L_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HGS0772235.1"
                     /translation="MSKKRRNRQKKEGQEPQFDFDEAKELTVGQAIRKNEEVEAGVLP
                     EDSILDKYVKQHRDEIEADKFATRQYKKEEFVETQSLDDLIQEMREAVEKSEASSEEV
                     PSSEDILLPLPLDDEEQGLDPLLLDDENPTEMTEEVEEEQNLSRLDQEDSEKKSKKGF
                     ILTVLALVSVIICVSAYYVYRQVARSTKEIETSQSTTANQSDVDDFNTLYDAFYTDSN
                     KTALKNSQFDKLNQLKTLLDKLEGSREHTLAKSKYDSLVTQIKAIQDVNAQFEKPAIV
                     DGVLDANAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSSSQASSNTTS
                     EPKPSSSNETRSSRSEVNMGLSSEGVAVQRSASRVAYNQSAIDDSNNSAWDFADGVLE
                     QILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGYFVGNGAGH
                     ADDLDY"
     gene            12392..13816
                     /gene="gndA"
                     /locus_tag="ACL73L_001261"
     CDS             12392..13816
                     /gene="gndA"
                     /locus_tag="ACL73L_001261"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HGS0772236.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIQALLPHLDKGDILIDGGN
                     TFYKDTIRRNEELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVSDVLEEIS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAE
                     IFTEWNKGELDSYLIEITADILSRKDDEDQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKEERVHASKVLPKPAAFNFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
     gene            13828..14517
                     /locus_tag="ACL73L_001262"
     CDS             13828..14517
                     /locus_tag="ACL73L_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGS0772237.1"
                     /translation="MGKRILLLEKERNLAHFLSLELQKEQYRVDLVEEGQKALSMALQ
                     TDYDLILLNVNLGDMMAQDFAEKLSRTKPASVIMILDHWEDLQEELEVVQRFAVSYIY
                     KPVFIENLVARISAIFRGRDFIDQHCSLMKVPRTYRNLRIDVEHHTVYRGEEMIALTR
                     REYDLLATLMGSKKVLTREQLLESVWKYESATETNIVDVYIRYLRSKLDVKGQKSYIK
                     TVRGVGYTMQE"
     gene            14615..15580
                     /gene="cbpC"
                     /locus_tag="ACL73L_001263"
     CDS             14615..15580
                     /gene="cbpC"
                     /locus_tag="ACL73L_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpC"
                     /protein_id="HGS0772238.1"
                     /translation="MKLLKKMMQVLLAVFFFGLLATNTVFANTTGGRFVDKDNRKYYV
                     KDDHKAIYWHKIDGKTYYFGDIGEMVVGWQYLEIPGTGYRDNLFDNQPVNEIGLQEKW
                     YYFGQDGALLEQTDKQVLEAKTSENTGKVYGEQYPLSAEKRTYYFDNNYAVKTGWIYE
                     DGNWYYLNKLGNFGDDSYNPLPIGEVAKGWTQDFHVTIDIDRSKLAPWYYLDPETGIM
                     RTGWQYLGNNWYYLRSSGAMATGWKYVRGKWYYLDNKNGDMKTGWYQDGSTWYYLDPS
                     NGDMKIGWTKVNGKWYYLNSNGAMVTGSQTIDGKVYNFASSGEWI"
     gene            15599..16597
                     /gene="cbpJ"
                     /locus_tag="ACL73L_001264"
     CDS             15599..16597
                     /gene="cbpJ"
                     /locus_tag="ACL73L_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpJ"
                     /protein_id="HGS0772239.1"
                     /translation="MKLLKKTMQAGLTVIFFGLLATNTVFADNSEGWQFVQENGRTYY
                     KKGDLKETYWRVIDGKYYYFDSLSGEMVVGWQYIPFPSKGSTIGPYPNGIRLEGFPKS
                     EWYYFDKNGVLQEFVGWKELEIKTQDSVGRKYGEKRENSEDKEEKRYYTNYYFNQNHS
                     LETGWLYDQSNWYYLAKTEINGENYIGGERRAGWINDASAWYYLDPATGIMQTGWQYL
                     GNKWYYLRSSGAMATGWYQEGTTWYYLDHPNGDMKTGWQNLGNKWYYLRSSGAMATGW
                     YQDGSTWYYLNAGNGDMKTGWFQVNGNWYYAYSSGALAVNMTVDGYSVNYNGEWVR"
     gene            complement(16715..17947)
                     /locus_tag="ACL73L_001265"
     CDS             complement(16715..17947)
                     /locus_tag="ACL73L_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGS0772240.1"
                     /translation="MKLFWTNNIYRQLLLNSCFSSFGDSIFYLAIINYVAQYNFAPLA
                     ILLISISEVVPLLSQLFLGILGDFQENRVKHALWIAKIKILLYAILTVFLVLSPFSLV
                     SVIMIVIINLISDTLSYLSAYMMNALYISVIKDDLHDAMGFRQSLMRVVRIVANLAGA
                     FLINVMSIQTISLINTLTFVIAFLGLYVIRHTLYEVEKRIEMSHTALSFKKYFQHLKQ
                     SLAVLLRLKDTVILLFLTTSMIAILDVSPRLIALRFIQQTLAQLSIGQLFSLLSIIMS
                     CGAILGNMTSSNLFKNIRFTHLLVFCEISLLALITSILCQAYIVIFVTSFISSTIIGI
                     LSPRLQAAVFAHIPSDKMGTVGSALSTVDILAPSLLSLLALSIASGVSVQLALIFLYL
                     ILIALIFCQWLVKFNTHN"
     gene            complement(17958..18410)
                     /locus_tag="ACL73L_001266"
     CDS             complement(17958..18410)
                     /locus_tag="ACL73L_001266"
                     /inference="COORDINATES: protein motif:HMM:NF017000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772241.1"
                     /translation="MFYNDLHTFHTELKKLLEKVTSNTENLGNLQLSWCKGISGIILY
                     LCMYDCDGNKDIISKYQEFVFNHHLKMMTGYCHGITSLLQTTVYNQNKLLMKKIQQVI
                     LACSERDDHGLLMFQGDSGKADLFDFGIGSMGVYWCLLNNKFPFDVQT"
     gene            complement(18478..18866)
                     /locus_tag="ACL73L_001267"
                     /pseudo
     CDS             complement(18478..18866)
                     /locus_tag="ACL73L_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865604.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase LanC family protein"
BASE COUNT         5974 a         3462 c         4366 g         5461 t
ORIGIN      
        1 cactttcaga ggaaggagta ttttttaaaa agaaaatgta aacgcttgct caaaaatgaa
       61 aggatttaga agtttatgaa taaaggatta tttgaaaaac gttgtaaata tagtattcgg
      121 aaattttcat taggtgttgc ttctgttatg attggagctg cattctttgg gacaagtccg
      181 gttcttgcag atagcgtgca gtctggttcc acggcgaact taccagctga tttagctact
      241 gctcttgcaa cagcaaaaga gaatgatggg cgtgattttg aagcgcctaa ggtgggagaa
      301 gaccaaggtt ctccagaagt tacagatgga cctaagacag aagaagaact attagcactt
      361 gaaaaagaaa aaccggctga agaaaaacca aaagaggata aacctgtagc tgctaaacct
      421 gaaacaccta agacggtaac ccctgaatgg caaacggtag agaaaaaaga acaacaggga
      481 acagtcgcta tccgagaaga aaaaggtgtc cgctacaacc aactatcctc aactgctcaa
      541 aatgataacg caggcaaacc agccctgttt gaaaagaagg gcttgaccgt tgatgccaat
      601 ggaaatgcaa ctgttgattt aaccttcaaa gaagattctg aaaagggcaa atcacgcttt
      661 ggtgtcttct tgaaatttaa agataccaat aataatgttt ttgtcggtta tgacaaggat
      721 ggctggttct gggagtataa atctccaaca actagcactt ggtatagagg tagtcgtgtt
      781 gctgctcctg aaacaggatc aacaaaccgt ctctctatca ctctcaagtc agacggtcag
      841 ctaaatgcca gcaataacga tgtcaatctc tttgacacag tgactctacc agctgcggtc
      901 aatgaccatc ttaaaaatga gaagaagatt cttctcaagg cgggctctta tgacgatgag
      961 cgaacagttg ttagcgttaa aacggataac caagaggggg taaaaacaga ggatacccct
     1021 gctgaaaaag aaacaggtcc ggttgttgac gatagcaagg tgacttatga cacgattcag
     1081 tctaaggtcc tcaaagcagt gattgaccaa gccttccctc gtgtcaagga atacagcttg
     1141 aatggacata ctttgccagg acaggtgcaa cagttcaacc aagtctttat caataaccac
     1201 cgaatcaccc ctgaagtcac ttataagaaa atcaatgaga caacagcaga gtacttgatg
     1261 aagcttcgcg atgatgctca cttaatcaat gcggaaatga cagtacgctt gcaagttgtg
     1321 gacaatcaat tgcactttga tgtgaccaag attgtcaacc acaatcaagt cactccaggt
     1381 caaaagattg atgacgaaag aaaactactt tcttctatta gtttcctcgg caatgcttta
     1441 gtctctgttt ctagtgatca aactggtgct aagtttgatg gggcaactat gtcaaacaat
     1501 acgcatgtca gtggagatga tcatatcgat gtaaccaatc caatgaaaga cctagccaag
     1561 ggttacatgt atggatttgt ttctacagat aagcttgctg ctggtgtttg gagtaactct
     1621 caaaacagct atggtggtgg ttcgaatgac tggactcgtt tgacagctta taaagaaaca
     1681 gtcggaaatg ccaactatgt aggaatccac agctctgaat ggcaatggga aaaagcttat
     1741 aagggcattg ttttcccaga atacacgaag gaacttccga gtgctaaggt tgttatcact
     1801 gaagatgcca atgcagacaa gaaagttgat tggcaagatg gtgccattgc ttatcgtagc
     1861 attatgaaca accctcaagg ttgggaaaaa gttaaggata tcacagctta ccgtatcgcg
     1921 atgaactttg gttctcaagc acaaaatcca ttccttatga ccttggatgg tatcaagaaa
     1981 atcaatctcc acacagatgg tcttgggcaa ggtgttctcc ttaaaggata tggtagcgaa
     2041 ggccatgact ctggtcactt gaactatgct gatattggta agcgtatcgg tggtgtcgaa
     2101 gacttcaaga ctctaattga gaaggctaag aaatatggag ctcatctagg tatccacgtt
     2161 aacgcttcag aaacttatcc tgagtctaaa tacttcaatg aaaaaattct ccgtaagaat
     2221 ccagatggaa gctatagcta tggttggaac tggctagatc aaggtatcaa cattgatgct
     2281 gcctatgacc tagctcatgg tcgtttggca cgttgggaag atttgaagaa aaaacttggt
     2341 gacggtctcg actttatcta tgtggacgtt tggggtaatg gtcaatcagg tgataacggt
     2401 gcctgggcta cccacgttct tgctaaagaa attaacaaac aaggctggcg ctttgcgatc
     2461 gagtggggcc atggtggtga gtacgactct accttccatc actgggcagc tgacttgacc
     2521 tacggtggct acaccaataa aggtatcaac agtgccatca cccgctttat ccgtaaccac
     2581 caaaaagatg cttgggttgg ggactacaga agttatggtg gtgcagccaa ctatccactg
     2641 ctaggtggct acagcatgaa agactttgaa ggctggcaag gaagaagtga ctacaatggc
     2701 tacgtgacta acctctttgc tcatgacgtc atgactaagt acttccaaca cttcactgta
     2761 agtaaatggg aaaatggtac accggtgact atgaccgata acggtagcac ctataaatgg
     2821 actccagaaa tgcgagtgga gttggtagat gctgacaata ataaagtagt tgtaactcgt
     2881 aagtcaaatg atgtcaatag cccacaatat cgcgaacgta cagtaacgct caacggacgt
     2941 gtcatacaag atggttcagc ttacttgact ccttggaact gggatgcaaa tggtaagaaa
     3001 ctttctactg agaaggaaaa gatgtactac ttcaatacgc aggccggtac aacaacttgg
     3061 acccttccaa gcgattgggc aaagagcaag gtttaccttt acaagctaac tgaccaaggt
     3121 aagacagaag agcaagaact aactgtaaaa gatggtaaaa ttaccctaga tcttctagca
     3181 aatcaaccat acgttctcta tcgttcgaaa caaaccaatc ctgaaatgtc atggagtgaa
     3241 ggcatgcaca tctatgacca aggatttaat agcggtacct tgaaacattg gaccatttca
     3301 ggcgatgctt ctaaggcaga aattgtcaag tctcaagggg caaacgatat gcttcgtatt
     3361 caaggaaaca aagaaaaagt tagtctcact cagaaattaa ctggcttgaa accaaatacc
     3421 aagtatgccg tttatgtcgg tgtcgataac cgtagtaatg ccaaggcgag catcactgta
     3481 aacactggtg aaaaagaagt gactacttat accaataagt ctctcgccct caactatgtt
     3541 aaggcctacg cccacaatac acgtcgtaac aatgctacag ttgacgatac aagttacttc
     3601 caaaacatgt acgccttctt tacaactgga tcggacgtat caaatgttac tctgacattg
     3661 agtcgtgaag ctggtgatga agcaacttac tttgatgaaa ttcgtacctt tgaaaacaat
     3721 tcaagcatgt acggagacaa gcatgataca ggtaaaggca ccttcaagca agactttgaa
     3781 aatgttgctc agggtatctt cccatttgta gtgggtggtg tcgaaggtgt cgaagacaac
     3841 cgcactcact tgtctgaaaa acacgatcca tatacacaac gtggttggaa tggtaagaaa
     3901 gtcgatgatg ttatcgaagg aaattggtca ctcaagacaa atggactagt gagtcgtcgt
     3961 aacttggttt accaaaccat cccacaaaac ttccgctttg aagctggtaa gacctaccgt
     4021 gtaacctttg aatacgaagc aggttcagac aatacctatg cttttgtagt cggtaaggga
     4081 gaattccaat caggtcgtcg tggtactcaa gcaagcaact tggaaatgca tgaattgcca
     4141 aatacttgga cagattctaa gaaagccaag aaggtaacct tccttgtgac aggtgcagaa
     4201 acaggggata cttgggtagg tatctactca actggcaatg caagtaatac tcgtagtgat
     4261 tctggtggaa atgccaactt ccgtggttat aacgacttca tgatggataa tcttcaaatc
     4321 gaagaaatta ccctaacagg taagatgttg acagaaaatg ctctgaagaa ctacttgcca
     4381 acggttgcca tgactaacta caccaaagag tctatggatg ctttgaaaga ggcggtcttt
     4441 aacctcagtc aggccgatga tgatatcagt gtggaagaag cgcgtgcaga gattgccaag
     4501 attgaagcct tgaagaatgc tttggttcag aagaaaacgg ctttggtagc agatgacttt
     4561 gcaagtctta cagctcctgc tcaggctcaa gaaggtcttg caaatgcctt tgatggaaac
     4621 ttatctagtt tatggcatac atcatggggc ggaggagatg taggcaagcc tgcaaccatg
     4681 gtcttgaaag aaccaactga aataactgga cttcgttatg ttccacgtgg atcaggttca
     4741 aatggtaact tgcgtgatgt gaaacttgtt gtgacagatg agtctggcaa ggagcatacc
     4801 tttactgcaa ctgattggcc agataacaat aagtcaaaag atattgactt tggtaagaca
     4861 atcaaggcta agaaaattgt ccttactggt accaagactt acggagatgg tggcgataaa
     4921 taccaatctg cagcggaact catctttact cgtccacagg tagcagaaac acctcttgac
     4981 ttgtcaggct atgaagcagc tttggctaag gctcagaaat taacagacaa agacaatcaa
     5041 gaggaagtag ctagcgttca ggcaagcatg aaatatgcga cggataacca tctcttgacg
     5101 gaaagaatgg tggaatactt tgcagattat ctcaaccaat taaaagattc tgctacgaaa
     5161 ccagatgctc caactgtaga gaaacctgag tttaaactta gctctttagc ttccgatcaa
     5221 ggtaagacgc cagattataa gcaagaaata gctagaccag aaacacctga acaaatcttg
     5281 ccagcaacag gtgagagtca atctgacaca gccctcttcc tagcaggtgt tagcctagcc
     5341 ctatctgctc tctttgtagt aaaaacgaag aaagactagt atttagtaaa acctcttaac
     5401 aagattacgg aagcagtctc tatcttttcc aatgaggttt atagtacaga aaaagcctga
     5461 gaagatgtct tctcaggctt ttgttaagca cataaataca atagtgctat gacaaaatca
     5521 cctagaaaaa tctgggtgat aaatgttatg gttgtgctgg ttgaggattc tgattttgtt
     5581 gatcaggggt tgtatttgat tgttgcgtat tattgttagg attggtagtc gtactattat
     5641 ttgtgcttgg agtggttgag ctagactgtg aagttgaact atctgatgat gagcttgaac
     5701 tttcagttga tgggggttgt tgtggagcag gtgagttcca cgtagaacga gcaccatttt
     5761 taaatacgaa ttctccattt ctgtagagcc cctctggtat attccaatct tctggattgc
     5821 ttccttcaga caggtaggtc atcatagagc ggtaaacttt ggcagcgacc gtaaggccat
     5881 tgcctacaag tggtgtcaga cggttagaat agcctgtcca tacagccatt gaatatttac
     5941 gcgtatagcc agcaaatagt tcatcaggtg ctacaaattg agaggtcttg atgtggtttt
     6001 caatttcctc gtctgtatag ttagaggttc ctgttttacc agcctgagga agccaagcaa
     6061 gataggcatt tcgtccagtt ccataagtca agactgtttt catcatgtcg gtcatcatat
     6121 aggctgtcgt ttctttcatg gcacgagttc cgacattaga gaactctttt tcactcccat
     6181 cactaaagac gactttatgg atatacattg gtttatagta agttccacca tttgcaaagg
     6241 cagcgtaagc agcagccatc ttttcactac ttgctccata ttttttgtct gattcggttg
     6301 tgttacttga aatggcattt gagtagtgaa tacttgggta gtcgattccg agaccattta
     6361 ggaaagtctt ggcgcggttg agtccgacct tgtttagagt ttccacggct gggacgtttc
     6421 gcgattgttg cagggcgtat tgcaaggtga tgttgccaaa gtagccccta tcccagttat
     6481 aaacaggagt atttgtccca gggtaattat agggctcatc gtgaacgata gtagcagttg
     6541 aatcgtagac accgtactcc aaggcaggag catagtctgt gatcggtttc atagttgatc
     6601 cccagtcgcg gtttgtttct actgcttggt taattccgaa ggaaacatta cttgactgat
     6661 ggcgtgctcc tagctgggca atgactttac cgttagaaac atcaacaatg gtagaagcga
     6721 cttgcaattc atcgtctgga taggcaacgt attcgtctgt attgtaaata tcccacagat
     6781 gtttttgagc ttcttggtct acatttgtgt agacatccat cccagttgtg agcaggttat
     6841 agcctgtttc ttcttcaact tgattgatga cttccttgag gtaattatcc atgtaagcag
     6901 ggtaattact tgctgatttg agactttgta gtccatcagt aattggtgta ttgactgctt
     6961 tctcatactg ttcagcagag atgtagcctt gatttttcat ttcagataag accaagtttc
     7021 ggcggtcttg ggctgcttct ggatgtgaat aggggtcata ttggtttggt gcctgaggca
     7081 ttccagccag caaggctaac tgaggtaaac ttaaattatt gaggtcttta ccatagtagt
     7141 tttgagctgc tgtctgcatt ccatagttcc cattagacat gtagacctta tttatatagt
     7201 aggtcaagat ttcttgcttg gttgcttttt gttctaactg aatcgctaac caagcttcct
     7261 gagccttacg agaaatagtc tggtcggaag tcgaagttga aaagtaagtc aacttaatca
     7321 actgttgggt gagagttgat ccaccttgga gggaattgct ttgcagattg cgcaagaaag
     7381 ctcccaggat acggatggta tcaatccccc tgtggtcgaa gaagcgatgg tcttcgatag
     7441 aaacgattgc cttaaccaaa tctgtgggaa tatcattagc ttgggcattg acgcggcgtt
     7501 cagaacccaa gtcagcaatg agttgatttt tattgtcgta gattttacta gaagttgttg
     7561 caactagttt actctcggat aggctaggag ccttgctaac gtagtagaaa aaaactcctc
     7621 cgcctaagac aatggctgcg ataaccaagc ttaagaagct aatgctcaga tacttgatta
     7681 ggcgcagaat cgttggtttg ttcatcttgt tttaccacct aataaatgtt ctttgataac
     7741 attgagataa ggaatttgag ggaaggcacc agccttgatt tcatatccat attctcgaat
     7801 atattcaagt ggcattgatt tttgtccctt atcttgatga tagaagcgaa tcaaatcgaa
     7861 tgccggcaat aagtaggttt cttgctgaga agaaaagtga agaaggacaa agcagattcc
     7921 ttgttgggca aggacttgtt ccatatgctg aatctgatgt ggatgaaaat ttttcatcgg
     7981 aatcgcacgt ttttgttttg tttccttgac ttcaaagtcg atgtaatatc cattataaac
     8041 gccagaatag tccgtcgttg aagcttgtcg aaaataggct tcaacaatct tggcacgact
     8101 tcgttgtgga tagtccactt gtacgatttg aataggagtt ggtttcttat gtataacagc
     8161 caagccctga gacaaatagt agtcgttggt agcattgatc atcttttcaa aagacattcc
     8221 tcgatttgcg aaatttttgg gttgagaaag agatgtttgt cttttttgtg atgaaacttt
     8281 atgtggatag ttgaccataa ttctccttat tggtacaata acatcacttt attataccat
     8341 aatttgcaca gaaagggtta aaaatggcta cagctttggt tttaggctat tctgcttttg
     8401 atttgggttt attttctgat aaggatcctc gtcttaaatt gattaaaaaa gcgatacgta
     8461 aagatttaga ggctatggca gcagatgggg tgtcttggct tgtgtttaca ggaagtttgg
     8521 gttttgaata ttgggtttta gaggttgctc aggagatgaa aaccgaatac ggtttccagt
     8581 tggccaccat ttttgctttt gaaacccatg gggaaaattg gaatgaaggc aatcagatga
     8641 aactgagtcg ctttaagcag gtagactttg tcaaatatgc ttatcctcgc tatgaacaca
     8701 aggggcagtt aagagattac cagcagtttt tgctggaaaa cacgactagt tcctatcttt
     8761 tttatgatga agaaaatgaa acaaaactag cttattttta ccaaaagatg aaaaatcaag
     8821 aggactattt tataaagaga ttaacatttg atcaattaaa tgaacttgct gaaaattttt
     8881 ccgaaaattg aagctttgac cttgattttt gtttgccttt ttttatataa taatactagc
     8941 aagcgagaat ggagagagac atggcaagta ttattttttc agcgaaagat atttttgaac
     9001 aagagtttgg acgtgaagtc cgtggctata ataaagtaga agttgacgag tttttagacg
     9061 atgtcatcaa ggactatgaa acctatgctg ccttggtcaa gtcacttcgt caggaaattg
     9121 cggatttgaa ggaagaatta actcgtaaac cgaaaccttc accagttcaa gcagaacccc
     9181 ttgaagcggc aattacaagt tctatgacga attttgatat tttgaaacgc ctgaatagat
     9241 tggaaaaaga agtttttggt aaacaaattt tagataactc agatttttaa gtagttattt
     9301 gagatgtgca atttttggat aatcgcgtga ggagaattgc ttctcatgag gaaagtccat
     9361 gctagcacag gctgtgatgc ctgtagtgtt tgtgctaggc gaaaccataa gcctagggac
     9421 gaggaatcgt tacggcagtt gaaatggcta agtccttgga taggccagag taggcttgaa
     9481 agtgccacag tgacgaagtc tttctggaaa cagagagagt ggaacgcggt aaacccctca
     9541 agctagcaac ccaaattttg gtcggggcat ggagtacgcg gaaacgaacg tagtattctg
     9601 actgctatca gctagagctg ttagtggtag acagatgatt atcgaaggaa gtggtcctag
     9661 tcacttctgg aacaaaacat ggcttataga aaattgcata taggttgggg ctgagaaatt
     9721 ttctcaacct cattttttaa agtggacata tagaaaggtc ttgcaagact gtaacatgaa
     9781 aaaagaattt aatttaattg caactgcggc agcagggctt gaggctgtcg ttggtcgtga
     9841 agtgcgagag ttgggctacg attgtcagat tgaaaatgga cgtgttcgtt ttcaaggaga
     9901 cgtgagagct attatcgaaa ccaacctttg gctgcgggca gcagatcgta tcaaaattat
     9961 cgtaggaacg ttccctgcta agacttttga agagctattt cagggagttt tcgctttaga
    10021 ttgggaaaat tatttaccac ttggagctcg gttcccgatt tcaaaagcta aatgtgttaa
    10081 gtccaaactt cacaatgagc ccagtgttca ggctatttct aagaaagctg ttgtcaagaa
    10141 attgcagaaa cactatgctc gcccagaagg ggttcctctg atggagaatg gcccagagtt
    10201 taagattgag gtctctattc tcaaagatgt ggcaactgtc atgattgata cgaccgggtc
    10261 tagcctcttt aaacgtggtt atcgtaccga aaaaggtggc gctcctatca aggaaaatat
    10321 ggcagcagcc attttacaac tttctaactg gtatccagac aagcctttga ttgatccgac
    10381 ctgtggttcg gggactttct gtattgaggc agttatgatt gctagaaaga tggcgccagg
    10441 tcttcgtcgc tcttttgcat ttgaggaatg gaactggatc agcgatcgct tgattcaaga
    10501 agtgcgcaca gaagcggcta aaaaagtaga ccgtgagctt gagctggata tcatgggctg
    10561 tgatattgat gctcgcatgg tggaaattgc taaggccaat gctcaggcag ctggtgttgc
    10621 aggagacatt acttttaagc agatgcgcgt gcaggattta cgttccgata aaatcaatgg
    10681 agtaatcatt tccaatccgc cttatggtga acgtttgtca gatgatgcag gggtgaccaa
    10741 gctctatgct gagatggggc aagtatttgc accgctgaaa acttggagca aatttatcct
    10801 gactagtgat gaagcttttg aaagcaagta tggtagccaa gcagataaga agcgtaagtt
    10861 atacaacgga accttgaaag tggatctgta tcaatatttt ggtcagcgtg tcaaacggca
    10921 agaggtaaaa tagaaaggga tactcatgag taaaaaaaga cgaaatcgtc aaaaaaaaga
    10981 aggtcaagaa ccgcaatttg attttgatga agcaaaagag ctaacagttg gtcaagctat
    11041 tcgtaaaaat gaagaagtgg aagcaggagt cttgcctgag gattccattt tggacaagta
    11101 tgttaagcaa cacagagatg aaattgaggc ggataagttt gcgactcgtc aatacaaaaa
    11161 agaggagttc gttgaaactc agagtctgga tgatttaatt caagagatgc gtgaggctgt
    11221 agagaagtca gaagcttctt cggaggaagt tccatcttct gaagacatct tactaccctt
    11281 gcctctggac gatgaggagc aaggcttgga tcctctattg ctagatgatg aaaatccaac
    11341 agaaatgact gaagaagtgg aagaggagca aaacctttct cgtctggatc aagaggactc
    11401 agaaaagaaa agtaaaaaag gctttatttt gaccgttttg gcgcttgtat cagtaattat
    11461 ttgtgtcagt gcttattatg tctaccgtca agtggctcgt tcgaccaagg aaattgaaac
    11521 ttctcaatca actacagcca atcaatcgga tgtggatgat tttaatacac tttatgacgc
    11581 cttttacaca gatagcaata aaacggcttt gaaaaatagc cagtttgata aactgaatca
    11641 actcaagact ttacttgata agctggaagg tagtcgtgaa catacgcttg ccaaatctaa
    11701 atatgatagt ctagtaacgc aaatcaaggc tattcaagat gtcaatgctc aatttgagaa
    11761 accagctatt gtggatggtg tgttggatgc caatgccaaa gccaaatcgg atgctaaatt
    11821 tacggatatt aaaactggaa atacggagct tgataaagtg ctagataagg ctatcagtct
    11881 tggtaagagc cagcaaacaa gtacttctag ctcaagttca agtcaagcaa gctcaaatac
    11941 gacaagtgag ccaaaaccaa gtagttcaaa tgagactaga agtagtcgca gtgaagtcaa
    12001 tatgggtctc tcgagtgaag gggttgctgt tcaaagaagt gccagtcgtg ttgcctataa
    12061 tcagtctgct attgatgata gtaataactc tgcctgggat tttgcggatg gtgtcttgga
    12121 acaaattcta gcgacttcac gttcacgtgg ctatatcact ggagaccaat atatccttga
    12181 acgtgtcaat atcgttaacg gcaatggtta ttacaacctc tataagccag atggaaccta
    12241 tctctttacc cttaactgta agacaggcta ctttgtcgga aatggcgctg gtcatgcaga
    12301 tgacttggac tactaagcag tcgttacaaa attctttctt ttcaaaagta aaaatgataa
    12361 aataaaacaa atgaaacaag aggagtgtca aatgacaaaa gctaactttg gtgtcgtagg
    12421 tatggccgta atgggtcgta accttgccct taatattgaa tcacgtggtt acacagttgc
    12481 tatctacaac cgtagtaaag aaaaaacgga agatgtgatt gcttgccatc ctgaaaagaa
    12541 ttttgtacca agctatgacg ttgaaagttt tgtaaactca atcgaaaaac ctcgtcgtat
    12601 catgctgatg gttcaagctg gacctggtac agatgctact atccaagccc ttcttccaca
    12661 ccttgacaag ggtgatatct tgattgacgg aggaaacact ttctacaaag ataccatccg
    12721 tcgtaatgaa gaattggcaa actcaggtat caactttatc ggtactggag tttctggtgg
    12781 tgaaaaaggt gcccttgaag gtccttctat catgcctggt ggacaaaaag aggcctacga
    12841 attggtttcg gatgttcttg aagaaatctc agctaaagca ccagaagatg gcaaaccatg
    12901 tgtgacttat atcggtcctg atggagctgg tcactatgtg aaaatggttc acaacggtat
    12961 cgagtacggt gatatgcaat tgatcgcaga aagctatgac ttgatgcaac acttgctagg
    13021 cctttctgca gaggatatgg ctgaaatctt tactgagtgg aacaagggtg aattggatag
    13081 ctacttgatc gaaatcacag ctgatatctt gagccgtaaa gacgatgaag accaagatgg
    13141 accaatcgta gactacatcc ttgatgctgc aggtaacaag ggaactggta aatggacgag
    13201 ccaatcatct cttgaccttg gtgtaccatt gtcactgatt actgagtcag tgtttgcacg
    13261 ctacatttca acttacaaag aagaacgtgt acatgctagc aaggtgcttc caaaaccagc
    13321 tgccttcaac tttgaaggag acaaggctga attgattgaa aaaatccgtc aagcccttta
    13381 cttctcaaaa atcatttcat acgcacaagg atttgctcaa ttgcgtgtag cctctaaaga
    13441 aaacaactgg aacttgccat ttgcagatat cgcatctatc tggcgtgatg gctgtatcat
    13501 ccgttctcgt ttcttgcaaa agattacaga tgcttacaac cgcgatgcag atcttgccaa
    13561 ccttcttttg gacgagtact tcttggatgt tactgctaag tatcaacaag cagtgcgtga
    13621 tatcgtagct cttgcggttc aagctggtgt gccagtgcca actttctcag cagctattac
    13681 ttactttgat agctaccgtt cagctgacct tccggctaac ttgatccaag cacaacgtga
    13741 ctactttggt gctcacactt accaacgtaa agacaaagaa ggaaccttcc actactcttg
    13801 gtatgacgaa aaataagtag gtcagccatg gggaaacgga ttttattact tgagaaagaa
    13861 cgaaatctag ctcatttttt aagtttggaa ctccagaaag agcagtatcg ggttgatctg
    13921 gtagaggagg ggcaaaaagc cctttccatg gctcttcaga cagactatga tttgatttta
    13981 ttgaacgtta atctgggaga tatgatggct caggattttg cagaaaaatt gagtcgaact
    14041 aaacctgcct cagtcatcat gattttagat cattgggaag acttgcaaga agagctggaa
    14101 gttgttcagc gttttgcagt ttcatacatc tataagccag tctttatcga aaatctggta
    14161 gcgcgtattt cggcgatctt ccgaggtcgg gacttcattg atcaacactg cagtctgatg
    14221 aaagttccaa ggacctaccg caatcttagg atagatgttg aacatcacac ggtttatcgt
    14281 ggtgaagaga tgattgctct gacccgccgt gagtatgacc ttttggcgac acttatggga
    14341 agcaagaaag tattgactcg tgagcaactg ttggaaagtg tttggaagta tgaaagtgcg
    14401 accgagacaa atatcgtaga tgtctatatc cgctatctac ggagcaagct tgatgttaaa
    14461 ggtcaaaaaa gctacattaa aaccgtgcgt ggtgttggtt acaccatgca agaatagaaa
    14521 agcagttgca gttgtgtaac tgcttttttg aggagttttt atatattgac atgcagtcgg
    14581 gtctttgcta caatcgtttt aggaggatat atagatgaaa cttttgaaaa aaatgatgca
    14641 agttctacta gcagtctttt tctttggttt gctagctaca aatacggtat ttgcgaatac
    14701 cacaggtggc cgatttgttg ataaggataa tagaaaatat tatgtaaaag atgatcataa
    14761 agcaatctat tggcataaaa tagacggtaa aacttactat tttggtgata ttggagagat
    14821 ggttgtcggt tggcaatact tagaaattcc tggaacaggt tatcgtgata atttattcga
    14881 taaccaacca gttaatgaaa ttggccttca ggagaagtgg tactattttg gacaagatgg
    14941 tgctttgcta gaacaaacag ataaacaagt actagaggca aaaacgtctg aaaatacagg
    15001 aaaagtatac ggtgaacaat atcctctatc tgctgaaaag agaacttatt attttgataa
    15061 taattatgct gtaaagacag gctggattta tgaagacggc aattggtatt atttaaataa
    15121 gctaggaaat tttggcgatg attcttacaa tccactacca attggtgaag ttgctaaggg
    15181 ttggactcaa gattttcatg ttactattga cattgataga agcaaacttg ctccatggta
    15241 ctacctagac ccagaaactg gcattatgcg aacaggatgg caatatctag gtaataactg
    15301 gtactacctc cgttcatcag gagcaatggc aacaggttgg aaatatgtac gaggcaaatg
    15361 gtattactta gataataaaa atggtgatat gaagactggt tggtatcaag atggttcaac
    15421 ttggtactat ttagatcctt ctaatggaga tatgaaaata ggttggacaa aagtaaatgg
    15481 aaaatggtat tatctcaatt caaatggagc aatggttaca ggtagtcaaa ctatcgatgg
    15541 taaagtttat aacttcgctt catctggtga gtggatttaa tgttggagga tatataaaat
    15601 gaagcttttg aaaaaaacta tgcaagctgg actaacagta attttctttg gtttgctagc
    15661 tacgaataca gtatttgcag ataactctga aggatggcag tttgtccaag aaaacggtag
    15721 aacctattac aaaaaggggg acctcaaaga aacctactgg cgagtgattg atggtaagta
    15781 ctattatttt gattctctat ctggagagat ggttgtcggc tggcaatata tcccgtttcc
    15841 atctaaaggt agtacaattg gtccttaccc aaatggtatc agattagaag gttttccaaa
    15901 gtcagagtgg tactactttg ataaaaatgg agtgctacaa gagtttgttg gttggaaaga
    15961 attagagatt aaaactcaag acagtgttgg aagaaagtat ggggaaaaac gtgaaaattc
    16021 agaagataaa gaagagaagc gttattacac gaactattac tttaatcaaa atcattcttt
    16081 agagacaggt tggctttatg atcagtctaa ctggtattat ctagctaaaa cagaaattaa
    16141 tggagaaaac tatattggtg gtgaaagacg tgcaggttgg ataaatgatg cttcagcttg
    16201 gtactaccta gatccagcaa ctggtattat gcaaacaggt tggcaatatc taggtaataa
    16261 gtggtactac ctccgttcct caggagcaat ggccactggc tggtatcagg aaggtaccac
    16321 ttggtattat ttagaccacc caaatggcga tatgaaaaca ggttggcaaa accttgggaa
    16381 caaatggtac tatctccgtt cctcaggagc tatggcaact ggttggtatc aagatggttc
    16441 aacttggtac tacctaaatg caggtaatgg agacatgaag acaggttggt tccaggtcaa
    16501 tggcaactgg tactatgctt atagctcagg tgctttggca gtgaatatga ccgtagatgg
    16561 ctattctgtc aactataatg gcgaatgggt tcggtaatga cagggcgctt ataattatat
    16621 tagaacactc gcttaatcaa agaagtgact ttgttgtact tgacctgacg attggggaga
    16681 ttaaaagcat gtgaaatcta cacatgcttt ttcgttagtt atgagtgttg aacttgacta
    16741 accattgaca aaagataaga gcaatcaaaa taagatacaa aaatatcaat gctaactgca
    16801 ccgaaacgcc cgatgctatg gatagggcta atagggagag cagggacggg gcgagaatgt
    16861 ctactgtgct cagagcagag cccaccgtcc ccatcttgtc actggggata tgggcaaaga
    16921 cagctgcttg taggcgaggg ctgagaatgc cgataatcgt agaactgatg aaactggtca
    16981 cgaaaattac gatataggct tgacaaagga tactagttat tagagccaat agggaaatct
    17041 cacagaaaac caagaggtgc gtgaaacgga tatttttaaa tagattactg ctggtcatat
    17101 tgccaaggat agctccacaa gacatgatga tggagagcag ggagaagagt tgcccaatgc
    17161 tcagttgtgc tagtgtctgt tggatgaagc ggagggcaat cagccgaggg gacacatcca
    17221 agatggcaat catactggtc gtcagaaaca gtagtatgac ggtatctttt aacctcagga
    17281 gcacagccag cgactgttta agatgttgaa aatatttctt aaaactcagt gctgtatgtg
    17341 acatttcaat ccttttttca acctcataca aggtatgtcg aataacatac aggcccaaaa
    17401 aggcaatgac aaaagtcaga gtgttgataa gggaaatagt ttgaatactc ataacattga
    17461 taaggaatgc gccagctaga ttggcgacaa tgcggacaac cctcatcaga gactgcctga
    17521 accccatggc atcatgcagg tcgtccttaa ttacactgat gtagagggcg ttcatcatgt
    17581 aggcagacag gtagctcaag gtgtcagaga tgaggttgat gatgacaatc ataatgactg
    17641 aaactaatga aaagggcgac aagacgagaa atactgtcaa aatagcgtag agcaggattt
    17701 tgattttggc aatccagagt gcgtgtttga ctctattttc ttgaaaatct cctagaattc
    17761 cgagaaagag ttgcgatagt aggggaacca cctctgaaat ggaaatcagt aaaatcgcta
    17821 gcggagcgaa attgtactga gccacataat tgataatggc aaggtagaaa atactgtcgc
    17881 cgaatgatga aaaacagctg tttagcaaca actgtctata tatgttgttt gtccaaaata
    17941 atttcataca tttctcctta tgtctgcaca tcgaatggga atttattatt taatagacac
    18001 caatataccc ccatgcttcc tattccgaag tcaaacaaat ctgctttacc actatctcct
    18061 tgaaacatca gtaaaccgtg atcatctcgt tcagaacatg ctaaaattac ctgttggatt
    18121 tttttcatca gtaatttgtt ttgattgtag acagtggttt gtagtaagct agttattccg
    18181 tggcaatatc ctgtcatcat ttttagatga tggttaaaaa caaattcttg atatttacta
    18241 ataatatctt tgtttccgtc acaatcgtac atacaaagat ataagattat tccggaaatt
    18301 cctttgcacc aagaaagttg taaatttcct aaattttcag tattagaagt aactttttct
    18361 aataattttt ttaattcagt atggaatgta tggagatcat tatagaacat agaaggttct
    18421 aagactttag aataggcaaa taaagcctat gcaattccac aatatccatg agcaaagcta
    18481 tagtctaaaa tgctttcttt ggctatatct ctttgtagga aataatgcta aatttcccct
    18541 atactattgt ggatgaaaat tttaagagta ttgtcattgg taaattgata gtattttatt
    18601 aatgataata ggacaccaga ttttcccaat gcaaagtcta tttcctctag agtatcataa
    18661 ttctctatta atttcttagc ggtgacgttt gctaattgta gatagtattg atttttactg
    18721 atagaaaata aatgtaggag taaccataga taacctgcat caccaaatag cagtgagtat
    18781 tgatattcat atgtgtgtgt cgttctaatt gtcactgcct tcttgatgat agagagtatt
    18841 ttagaatcca gttctgggtg gtacaattta tttagaggaa acagtagtcc agctattccg
    18901 tgttggaatg ataaattact tacgaaattt ccaaattctg ttgaagaagt gatccttcct
    18961 tcagaattta cagttttatc tagtaaaaat cgtagaacac tgttacattc tgttttgtaa
    19021 tcatatttat gttcctctat ttttttaatt ttgactaaat tatttgtttg acttaaattt
    19081 agtagacatt gtaaccaaaa tagttgctca tctgtgagct gaccttgagt atgtgatagt
    19141 tctagtaatt gaataggatc ctttagttag attcaatcaa tctataacac aacaacataa
    19201 taatactttt aggataactt atcaaatatt atagtaaaat gagataataa caggataaat
    19261 cga
//
LOCUS       DBERDK010000037        18912 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.037, whole genome shotgun sequence.
ACCESSION   DBERDK010000037 DBERDK010000000
VERSION     DBERDK010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 18912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18912
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.037"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..281)
                     /locus_tag="ACL73L_001268"
     CDS             complement(<1..281)
                     /locus_tag="ACL73L_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGS0772242.1"
                     /translation="MNFFSKLFNLKQNNHNRDTNSDCNNFYLNELECGLTPGQLILID
                     WTQKTGRNYNFPRYFKYSLQIDPESTHNQLYKLGYFTKNKTLSYLTVVE"
     gene            complement(324..1130)
                     /gene="yidA"
                     /locus_tag="ACL73L_001269"
     CDS             complement(324..1130)
                     /gene="yidA"
                     /locus_tag="ACL73L_001269"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HGS0772243.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDQGDYVVTFNGALVQETATGHEIISESLTYEDYLDMEFLSRK
                     LGVHMHAITKDGIYTANRNIGKYTVHESTLVSMPIFYRTPEEMAGKEIVKCMFIDEPE
                     ILDAAIEKIPAEFYERYSINKSAPFYLELLKKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRAMLEVVGNPVVMENGNPEIKKIAKYITKTNDESGVAHAIRTWVL"
     gene            complement(1143..2447)
                     /locus_tag="ACL73L_001270"
     CDS             complement(1143..2447)
                     /locus_tag="ACL73L_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HGS0772244.1"
                     /translation="MNEKVFRDPVHNYIHVNNQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTMTAALLHDLGHGAYS
                     HTFENLFDTDHEAITQEIIQNPETEIHQVLLQVAPDFPEKVASVIDHTYPNKQVVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPVENGIAFQRNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPEDKDFFARTSPHLLPFFEKNVTLTD
                     YLALDDGVMNTYFQLWMTSPDKILADLSHRFVNRKVFKSITFSQEDQDQLTSMRKLVE
                     DIGFDPDYYTAIHKNFDLPYDIYRPESENPRTQIEILQKNGELAELSSLSLIVQSLAG
                     SRHGDNRFYFPKEMLDQNSIFASITQQFLHLIENDHFTPNKN"
     gene            2519..2896
                     /locus_tag="ACL73L_001271"
     CDS             2519..2896
                     /locus_tag="ACL73L_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HGS0772245.1"
                     /translation="MKIRMRNTIQFDEQLEVIDQLYDVEVHEKGDYSYLLFYNEEKEK
                     VVIKFHGQELVMSRFSNPKTIMRFLKDSDSLAYIPTPMGMQEFIIQTSRYQVDGQKIE
                     LAYQLQNQEGHPFASYQLEITWG"
     gene            2986..3318
                     /locus_tag="ACL73L_001272"
     CDS             2986..3318
                     /locus_tag="ACL73L_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HGS0772246.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILERYPKDDFLQEQIEI
                     LTSIDNRE"
     gene            3330..4901
                     /gene="ffh"
                     /locus_tag="ACL73L_001273"
     CDS             3330..4901
                     /gene="ffh"
                     /locus_tag="ACL73L_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002902912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HGS0772247.1"
                     /translation="MAFESLTERLQNVFKNLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHEVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRPAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKALAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVRHITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKVDERQIARKRAIVSSMTPEERENPD
                     LLNPSRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMPDLSALGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(5096..6379)
                     /locus_tag="ACL73L_001274"
     CDS             complement(5096..6379)
                     /locus_tag="ACL73L_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HGS0772248.1"
                     /translation="MKQESTVDLLLDVDQRPSDGKGILLSFQHVFAMFGATILVPLIL
                     GMPVSVALFASGVGTLIYMIATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGV
                     ILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
                     KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLKANWFE
                     IPGFYLPFSTGGAFKEYNLYFGPEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEP
                     GLHRTLLGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIALSFL
                     GKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGL
                     GGAILKLGPVTLSGTALSAMTGIILNLILPYENKD"
     gene            complement(6682..7395)
                     /gene="rsmG"
                     /locus_tag="ACL73L_001275"
     CDS             complement(6682..7395)
                     /gene="rsmG"
                     /locus_tag="ACL73L_001275"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HGS0772249.1"
                     /translation="MKPKTFYNLLAEQNLPLSDQQKEQFERYFELLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
     gene            7489..8049
                     /locus_tag="ACL73L_001276"
     CDS             7489..8049
                     /locus_tag="ACL73L_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HGS0772250.1"
                     /translation="MTWIILGVIALIVIFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLIETVKGYAKYEGSTLEKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNVKLETFPSNIIAGMFG
                     FKAADFLQTPEEEKSVPKVDFSGLGD"
     gene            8051..8950
                     /gene="htpX"
                     /locus_tag="ACL73L_001277"
     CDS             8051..8950
                     /gene="htpX"
                     /locus_tag="ACL73L_001277"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HGS0772251.1"
                     /translation="MLFDQIASNKRKTWILLLVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGAREVDEQTAPDLYHVVEDMALVAQIPMPRVFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTIAV
                     ALASAITMLSSMAGRMMWWGGAGRRRSDDDRDGNGLEIIMLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSALYINDPKKG
                     GGFQKLFYTHPPISERIERLKQM"
     gene            complement(8994..10553)
                     /locus_tag="ACL73L_001278"
     CDS             complement(8994..10553)
                     /locus_tag="ACL73L_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HGS0772252.1"
                     /translation="MHYEHSRKGNHMIKINHLTITQNKDLRDLVSDLTMTIQDGEKVA
                     IIGEEGNGKSTLLKILMGEALSDFTIKGNIQSDYQSLAYIPQKVPEDLKKKTLHDYFF
                     LDSIDLDYSILYRLAEELHFDSNRFASDQEIGNLSGGEALKIQLIHELAKPFEILFLD
                     EPSNDLDLETVDWLKGQIQKTRQTVIFISHDEDFLSETADTIVHLRLVKHRKEAETLV
                     EHLDYDSYSEQRKANFAKQSQQAANNQRAYDKTMEKHRRVKQNVETALRATKDSTAGR
                     LLAKKMKTVLSQEKRYEKAAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLS
                     IDDRVLVQKLQLTVRGQEKIGIIGPNGVGKSTLLAKLQRLLNDKREISLGFMPQDYHK
                     KLQLDLSPIAYLSKTGEKEELQKIQSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDL
                     VLRKPNFLLLDEPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRFLKEVCSIIYRMTE
                     HGLKLVNLEDL"
     gene            complement(10792..11109)
                     /locus_tag="ACL73L_001279"
     CDS             complement(10792..11109)
                     /locus_tag="ACL73L_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HGS0772253.1"
                     /translation="MEDKKLIQLLSKLNKSYQNCKQGTADDIRLQELLNTTMQELKKT
                     EKLNNSILIDLEKFYQPTSPLIGLGSLKLNNQARTAWRNYDKIHYEHVKHVLSLYGPV
                     FGF"
     gene            11279..11821
                     /locus_tag="ACL73L_001280"
     CDS             11279..11821
                     /locus_tag="ACL73L_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HGS0772254.1"
                     /translation="MKLNIQEIRKQSEGLNFEQTLDLVDDLRARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGATNHLNQEILDDD
                     LVLPIENGALDLAESVSDNILLNIPIKVLTAEEEAGQGFVSGNDWQIMTEEEYQAQQA
                     VKKEENSPFASLQGLFDGDE"
     gene            11821..12450
                     /gene="nth"
                     /locus_tag="ACL73L_001281"
     CDS             11821..12450
                     /gene="nth"
                     /locus_tag="ACL73L_001281"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HGS0772255.1"
                     /translation="MVLSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQT
                     TDAAVNKATPGLFVAFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            12661..13182
                     /gene="pyrR"
                     /locus_tag="ACL73L_001282"
     CDS             12661..13182
                     /gene="pyrR"
                     /locus_tag="ACL73L_001282"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HGS0772256.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVTDREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            13201..14124
                     /locus_tag="ACL73L_001283"
     CDS             13201..14124
                     /locus_tag="ACL73L_001283"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HGS0772257.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
                     IAGDLDHSRVAKSNMQILKRLGAELFFAGPEEWRSQEFADYGQFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            14174..15253
                     /locus_tag="ACL73L_001284"
     CDS             14174..15253
                     /locus_tag="ACL73L_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HGS0772258.1"
                     /translation="MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLK
                     AKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEVFKQSN"
     gene            15565..18741
                     /gene="carB"
                     /locus_tag="ACL73L_001285"
     CDS             15565..18741
                     /gene="carB"
                     /locus_tag="ACL73L_001285"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HGS0772259.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKEGPDALLPTLGGQTGLNMAMEL
                     SKNGILDELGVELLGTKLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAVAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPAQTMSDYENQMLRDASLSIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPVTGSTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVHHNEVPELAAVSDDALIEKVVKAQDDRLFYVSEAIRRGYTPEEIAE
                     LTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLKTAKLNGFSDRKIAELWGTTDDKVRQ
                     LRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGWENESIRSDKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQP
                     PGQTATNEEEAALAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPDH
                     PVLVDSYIVGQECEVDAISDGKNVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQE
                     TIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVA
                     TKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     ATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAA
                     FFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEH
                     GVPLFTALDTANAMLKVLESRSFVTEAI"
BASE COUNT         5523 a         3632 c         4233 g         5524 t
ORIGIN      
        1 aattctacta ctgtaagata tgataaagtc ttatttttag tgaagtatcc taatttgtat
       61 aattgattgt gtgtagattc agggtcaatt tgaagagagt atttaaaata tcttgggaaa
      121 ttataatttc tccctgtttt ttgagtccaa tctatgagta tgagttgccc aggagtaagg
      181 ccacattcta attcgtttag gtaaaaattg ttacaatcag aatttgtgtc tctattatga
      241 ttattttgtt ttaaattaaa tagtttgcta aaaaaattca ttggatttta ttgctaatca
      301 attcttattg aaaaatgata cttttacagt acccatgttc ggatggcatg ggcaacgccg
      361 gattcgtcat ttgttttggt gatgtatttg gcgatttttt tgatttctgg atttccattt
      421 tccatgacaa cggggtttcc aacgacttcc agcatggcac ggtcattttc ttcatcaccg
      481 attgccatgg tttcatcttt ggtcaatccg agtttttcag ccaagtgagt aatggctgaa
      541 cccttgtcta cattcttttt aaggagttcg aggtagaaag gagcagattt gttgatggag
      601 tagcgctcgt aaaattctgc tggaattttt tcaatcgcag catcgagaat ttctggttca
      661 tcgataaaca tacatttaac aatttctttg ccagccattt cttcaggggt acggtagaag
      721 ataggcatgc tgacgagggt tgattcgtgt acagtgtatt ttccgatatt gcgatttgca
      781 gtatagatac cgtccttggt aatggcatgc atgtggacac cgagcttgcg actgaggaat
      841 tccatatcca gataatcctc ataggtcaag gattcgctga taatctcatg gccagtagca
      901 gtttcttgga caagggcacc attgaaggtc accacatagt caccttggtc tctcaactgc
      961 aagtcgtcca gaagtttggc aacacctgcg atggggcggc cagttgcaat cacgactttg
     1021 acaccagctt ctttggcatc ttggatggca gaaaagactt caggagtgat ttccttttgg
     1081 ctgttgacta gggttccgtc aatatcgacg gcaattagtt taatactcat aaaattcctc
     1141 ttctagtttt tatttggggt aaaatgatcg ttctcaatca agtgtaaaaa ttgctgggta
     1201 atgcttgcga agatgctgtt ttggtctaac atttctttag ggaaatagaa acgattatct
     1261 ccgtggcgac tgccagcaag ggattggacg ataagagaca ggctagagag ttcggccagt
     1321 tctccatttt tttgtaaaat ctcaatctgt gtccgtgggt tttcagattc gggacgatag
     1381 atatcataag ggaggtcaaa gttcttatga atggcagtgt agtagtcggg atcaaagccg
     1441 atatcctcaa ccaattttct catgctagta agttgatctt ggtcctcttg tgaaaaggta
     1501 atggatttaa agaccttgcg gttgacaaag cgatgcgata aatctgcaag aatcttgtca
     1561 ggactggtca tccaaagctg gaagtaggta ttcatcacgc catcatccag agccagatag
     1621 tcagtcaagg tcacattttt ttcgaagaaa ggcaggaggt gtggagaagt tcgggcaaag
     1681 aaatccttgt cctcaggata gagttccttg gcgcgcttga gaagattctg taggagaact
     1741 tccatggcgc gtgttgctgg gtggaaataa acctgcatgt acatctggta gcgactgagg
     1801 acgtagtctt cgatggcgtg catgccattg cgctgaaagg cgataccatt ttcgacagga
     1861 cgaatgactc ggaggatgcg agtcaggtca aattccccat aggatgctcc tgtaaaatag
     1921 gagtcgcgca agagatagtc catgcgatct gcgtcaatct gactagaaat gagttgtacg
     1981 acctgcttgt taggataggt atggtctatg acactggcaa ctttctctgg aaaatcgggc
     2041 gctacttgta gcaggacttg gtgaatctct gtctcaggat tttgaataat ctcctgagta
     2101 atggcttcat ggtctgtatc aaagagattt tcaaaagtat gggagtaggc accatgccca
     2161 aggtcgtgta ggagagcagc ggtcatggtc aagagagact cggcaggatt ccattcctca
     2221 ggatattttt cttcgaaaat ctctgtgatg cgtcgtgcaa tttcatagac tcctagacag
     2281 tgagagaagc gactgtgttc tccaccgtgg aaggtataac tggaagttcc cagttgtttg
     2341 atccggcgca aacgctgaaa ttcttttgta ttaatcaagt catagatgat ttgattattg
     2401 acatggatgt agttgtgaac tgggtcacgg aatacttttt cgttcatatg accattatag
     2461 caaaatcctg ctctttttgg taaaatagta ggacaagaga caagaaagag gacctgatgt
     2521 gaagattcgg atgcgaaata caattcagtt tgatgagcag ttggaagtga ttgaccagct
     2581 ttatgatgtg gaagtgcatg aaaaaggaga ttatagctac ctgcttttct ataatgagga
     2641 aaaggaaaaa gtagttatta aatttcatgg tcaagaactg gtgatgagcc gattttccaa
     2701 tcccaagacc attatgcgct ttttaaagga tagtgatagt ttggcctata ttcctacccc
     2761 tatgggcatg caggaattta tcattcaaac gagccgttat caagtagatg ggcaaaagat
     2821 tgagctagct tatcaactac aaaatcaaga ggggcatccc tttgccagct atcaattgga
     2881 aattacttgg ggctaggatc tcgtttttct caaatctact ttctatcttc aaggattggc
     2941 tccctagtgt ggtagattag gctatttttt ggtataataa aagttatgga aatcgaaaaa
     3001 accaatcgta tgaatgcgct ctttgaattt tatgcggcgc ttttgacaga taagcaaatg
     3061 aattatatag agctttacta cgctgatgat tacagtcttg ctgagatagc tgaggagttt
     3121 ggtgttagtc gtcaggctgt ctatgacaat atcaagcgaa cagaaaagat tctggaagat
     3181 tatgagatga aattgcacat gtactcggac tacattgtcc gtagtcagat ttttgaccaa
     3241 atcttggagc gctatcccaa ggatgatttt ctgcaggagc agatagaaat tttaacaagc
     3301 attgataata gagaataaga ggaagaaaca tggcatttga aagtttaaca gaacgtttgc
     3361 agaacgtctt taaaaatctc cgtaaaaaag gaaaaatctc agagtctgat gtccaagagg
     3421 caaccaaaga aattcgcttg gccttgcttg aggccgacgt tgctttgcct gttgtaaagg
     3481 actttatcaa gaaagttcgt gagcgtgcag tcgggcatga ggtcattgat acacttaatc
     3541 ctgcgcaaca gattattaaa atcgttgatg aggaactgac agccgtttta ggttctgata
     3601 cggcagaaat tatcaagtca cctaagattc caaccatcat catgatggtt ggtttacaag
     3661 gggctggtaa aacaaccttt gctggtaaat tggccaacaa actcaagaaa gaagaaaatg
     3721 ctcgtccttt gatgattgcg gcggatattt atcgtccagc tgccattgac cagcttaaga
     3781 ccttgggaca acagattgat gtgcctgtct ttgcacttgg aacagaagta ccagctgttg
     3841 agattgtacg tcaaggtttg gagcaagccc aaactaatca taacgactat gtcttgattg
     3901 atactgcggg tcgtttgcaa attgatgagc tcctcatgaa tgagcttcgt gatgtgaaag
     3961 cattggctca accaaatgaa attctcctcg tcgttgatgc catgattggt caggaagcag
     4021 ccaatgttgc gcgtgagttt aatgctcagt tggaagtgac tggggtcatc cttaccaaga
     4081 ttgatggcga tactcgtggt ggtgcggctc tatcggttcg tcacatcact ggaaaaccaa
     4141 tcaagttcac tggtacaggt gaaaaaatta cagatatcga aaccttccac ccagaccgta
     4201 tgtctagccg tatccttggc atgggggata tgctcacttt gattgagaaa gcttctcagg
     4261 aatacgatga acaaaaagcc cttgaaatgg ctgagaagat gcgcgaaaac acctttgatt
     4321 ttaatgattt catcgatcaa ttagatcagg tgcaaaatat ggggccgatg gaagacttgc
     4381 tcaagatgat tccaggtatg gccaacaatc cagcacttca aaacatgaag gtggatgaac
     4441 gccagattgc tcgtaaacgt gccattgtgt cttcgatgac acctgaagaa cgtgaaaacc
     4501 cagatttgtt aaatccaagc cgtcgccgtc gtattgctgc tggttctgga aatacattcg
     4561 tcgaagtcaa taaatttatc aaggacttta accaggctaa acagctcatg cagggtgtta
     4621 tgtctgggga tatgaataaa atgatgaagc aaatggggat taatccaaat aaccttccta
     4681 aaaatatgcc aaatatggga ggaatggata tgtctgccct tgaaggaatg atgggacaag
     4741 gcggtatgcc tgacttatca gctctcggag gagcaggaat gccagatatg agccagatgt
     4801 ttggtggcgg tttgaaaggt aaaattggtg aatttgccat gaaacagtcc atgaaacgta
     4861 tggctaacaa aatgaagaaa gcgaagaaga aacgcaagta agacaagaga ttgatgcaaa
     4921 tctcatttga tgagaaaata aagtaaaaat ttctatgata aaacgtacag tatcaaggtt
     4981 ttagaatatc tgatattata cgttttttga ttttaataat tttgttaaga cacttttatt
     5041 atgatatacg aaaaatccac tcagctgtct gagtggatta ggtgtattta gactcttagt
     5101 ctttattttc gtatggcaag atcaagttca agatgattcc tgtcatggct gaaagggcag
     5161 tacctgaaag tgtaactgga ccaagtttaa ggatagctcc tccaagtcca agaaccaaca
     5221 tagcacttgc gatgatgagg tttcgcattt gagcgaaatc aacacgttct ttaatcaaga
     5281 ctttcaaacc attgctggcg ataaccccat agagaaggat tgacatacca ccaagtacag
     5341 cgtttggaat agttgaaatc aaggcagtga atttaccaag gaagctgagg gcaatcgcga
     5401 tgaaggcagc gttacggata actgagacag aagcgatacg agtcataccg ataacccctg
     5461 tattttctcc gtaagttgta ttggctggtc caccaaggaa ggcagaaaca gaagttgcga
     5521 taccgtcacc aagaagagta cggtgaagac ctggttcttt taagaattga cgaccacaga
     5581 tttgacccaa aacagtatgg tctccgatat gttcagaaat tgttacgata gcgattggca
     5641 agatagcgat ggcttctgga ccaaagtaaa gattgtactc tttaaaggca ccacctgtgc
     5701 taaatggcaa gtagaaacca ggaatttcga accagttggc tttaagaact ggtgtaaagt
     5761 caaccaagcc aagagttagt gcgaaaaggt aaccaccgat aatggcaaag aggaatggaa
     5821 tgattcgtag gaagcctttt ccttttgtat tgataaaggc agcaattagg aaagtaacaa
     5881 cggctaccag agcatttttc caatttccgt ctgctacaag acctgcattg gtaacagctg
     5941 aacctgcaag tccaagaccg ataacgatga tcataggacc gataatgatt ggtggcaaga
     6001 gtttatcaat ccattttgtt cctacaaatc ggatgctggt agcaacaagg acatagacca
     6061 aaccagtcaa gataacccct gtttgggcag cagatacatc cccccccatt tctttcatag
     6121 ccagtgacat agctgtgata aaggcaaatg aagaacctag ataaactgga actttaaaac
     6181 cagtagcaat catgtagatg agtgttccaa cacctgaagc aaaaagggca acagatacag
     6241 gcattcccaa aatcaatggt accaagatgg tcgcaccaaa catggcgaaa acgtgttgga
     6301 agctaaggag aattcctttt ccatccgaag gacgttggtc aacgtctagt aacaaatcaa
     6361 cagttgattc ttgtttcata taaacctcac ttttgggcat caaaaaagag cccattattt
     6421 tacagcaata ggccctcaga gtaagacttc tttaaaattt agattttaaa gagtttcaaa
     6481 tatttctgcg tcttcgtcac ctcacgggat gacattaaaa acctttgctt aggatagtat
     6541 agcagaaaaa aatgattttg taaatcattt tttcccgagc ctagaaataa aagagcgagg
     6601 ctgattttgt aaatcatttt ttcccgagcc tagaaataaa agagcgaggc tgattttgta
     6661 aacatttgtt tactaaaaaa tttaaagtgg gcgtttattt ggcataccag ccttacgcgg
     6721 atatttattt ggtgtttctt ttttcttttc taccactgtg atatagcgcg gatctctatt
     6781 cggtagggcg tagctgagat tgtcttcgac cttactaaaa aggagattga gggcattctt
     6841 agcttctaat aattcctcag gcgcattgct ggccttgagt gccaatagtt ttccaccaac
     6901 cttaaggtag ggaatggtca attcagatag gacctgcata cgggcaaccg cacgagctgt
     6961 tacaaaatca tattgagcac ggaagttctt gtcttgggca aaatcttcgg cacgtccgtg
     7021 gtagaaatga actccgttca aatccagttc ttgagccaag agttgtagga agttgatgcg
     7081 cttattgagt gaatcaataa tggtcacatc taactccgga tagagaattt tcattggtag
     7141 actaggaaat cctgccccag ccccgatatc aagaagtttg atagtttcat tgggaatcaa
     7201 accttgaaga atgggtgcaa tcgaatcgta aaaatgtttg agataaactt cttccttgtc
     7261 cgtaatcgcc gtcaaattaa tcttctcatt ccactcgacc aagagctcaa aataacgttc
     7321 aaattgttct ttttgctggt ccgaaagtgg aagattctgc tcggcaagca agttgtaaaa
     7381 tgtttttggt ttcatagtta tttccttctt tagtatcatc ttattttacc acaatcatag
     7441 aaaatttgat atactggtac taatctaaaa gcagaaagga cttatattat gacttggatt
     7501 attcttggag ttatcgctct tattgttatt tttgtgattg ttagctataa cggtttggtt
     7561 aaaaatcgta tgcaaaccaa ggaggcttgg agtcagattg atgttcagtt gaaacgtcga
     7621 aatgacctct tgccaaactt gattgagact gtaaaaggtt atgccaaata tgaaggttct
     7681 acccttgaaa aggtggcaga actacgtaac caagtggcgg cagcgacttc accagcagaa
     7741 gctatgaaag ccagtgatgc cctcactcgt caggtttcag gtatttttgc agttgcagaa
     7801 agctatccag atttgaaagc tagtgctaac tttgttaaat tgcaagagga gttgacaaac
     7861 acagaaaata aaatttctta ctctcgtcaa ctctataaca gtgttgtcag caactacaat
     7921 gtaaaattag aaactttccc gagcaatatt atcgctggaa tgtttggatt taaagcggca
     7981 gatttccttc aaacacctga agaggaaaag tcggttccta aagttgattt tagcggttta
     8041 ggtgactaag atgttgtttg atcaaattgc aagcaataaa cgaaaaacct ggattttgtt
     8101 gctggtattt ttcctactct tagctcttgt tggttatgcg gttggttatc tctttataag
     8161 atctggactt ggtggtttgg ttattgcact gattatcggc tttatctacg ctttgtctat
     8221 gatttttcaa tcgacagaga ttgtcatgtc catgaatgga gcgcgtgagg tggatgagca
     8281 aacggcacca gacctctacc atgtagtgga agatatggct ctggtcgctc agattcctat
     8341 gccccgtgtt ttcatcattg atgatccagc cttaaatgcc tttgcgacag gttctaatcc
     8401 tcaaaatgcg gctgttgctg cgacttcagg tctactagct atcatgaatc gtgaagaact
     8461 agaagctgtt atgggacatg aagtcagtca tattcgtaat tatgatatcc gtatttcgac
     8521 tattgcagtt gcccttgcta gtgctatcac catgctttct agtatggcag gtcgtatgat
     8581 gtggtggggt ggagcaggtc gcagacgaag tgatgatgac cgagatggaa atggtcttga
     8641 aatcattatg ctagtggttt ccctactagc tattgtactg gcacctctcg ctgcaacctt
     8701 ggttcagctc gctatttctc gtcagaggga atttctggca gatgcatcta gtgtcgagct
     8761 gactcgcaat ccccagggaa tgattaatgc cctagataag ttggacaata gcaaacctat
     8821 gagtcgccac gtcgatgatg ctagcagtgc cctttatatc aatgatccta agaaaggtgg
     8881 ggggttccaa aaactctttt atacccaccc acctatctca gaacggattg aacgtttaaa
     8941 acagatgtaa aagaatccag aggtcgtctc tggatttttg ctatgttgca aatttataaa
     9001 tcttctaaat taactagctt caaaccgtgt tctgtcatgc gatagatgat cgagcagact
     9061 tcttttaaga aacgacggtc atgcgaaaca gtgatgagac cgcctggata ggtagcaaag
     9121 agttttctga tttggggttg agaagtggga gaaaagtttc gtgtgggttc atccagcagg
     9181 agaaagtttg gtttgcgcag gactaaatcc aaaagcagga gttttccctg ttgtccgcca
     9241 gataaggagc gaatttgatg ctgcatttct ggataactga aattgagact agctaggtga
     9301 gattggattt tctgtagttc ctctttttcc ccagttttac tgagataggc tattggggat
     9361 aaatccaatt gcagtttttt gtggtaatct tgtggcataa aaccaagtga aatctctctt
     9421 ttatcattca gaagtctctg taacttggct aacagagttg atttcccaac accatttggc
     9481 ccgataatac cgattttttc ttggccacgg acagttagtt gtagtttttg aaccaaaact
     9541 cggtcgtcaa tggacaaatt ttctttttcc agttggacta agactttaga agctggtaat
     9601 ggttggatgt ctgaaaagaa aagttggatt tgttcctctt caagtggctt ttgagtcatg
     9661 gactgagctg ccttttcgta gcgtttttct tgtgagagga cagttttcat ctttttagcc
     9721 aataggcgac cggcagtact atctttggta gctcgaagcg cagtttctac attttgctta
     9781 actctccgat gtttttccat ggttttatcg taggctcttt ggttgttagc agcttgctga
     9841 ctttgtttgg caaaattagc ctttctctgc tcactatagc tatcatagtc taaatgctct
     9901 actagcgttt ccgcttcttt acggtgtttg accagtcgca agtgaacaat agtgtctgcc
     9961 gtttcagaaa gaaagtcttc atcatgggaa atgaaaataa cggtttgcct ggtcttttga
    10021 atctggcctt ttagccaatc aactgtctca aggtctaggt catttgaagg ttcatctaaa
    10081 aatagaatct caaagggttt ggctaactca tggataagct gaattttcaa agcttcgccc
    10141 cctgatagat tgccaatctc ttggtcactt gcgaaacgat tgctatcaaa atgcaattcc
    10201 tccgccaaac gatagaggat actgtagtct aaatcaatag aatctaaaaa gaagtagtcg
    10261 tgtaaagttt tcttttttag gtcctcaggg actttttgag gaatgtaggc cagtgactga
    10321 tagtcagatt ggatgtttcc cttgatagtg aaatcagaca aagcttcccc cattaaaatt
    10381 ttaagtaagg ttgatttgcc atttccttct tcaccaataa tagcaacctt ttccccgtct
    10441 tggatggtca tggttaggtc agatacaaga tctcgtaaat ctttgttttg tgtgatggtt
    10501 agatgattga tttttatcat atgatttccc tttctagaat gttcatagtg cataacaaaa
    10561 agctctactt tacctagtag agcccaaagt ctatgtcaaa aaaactctat taacatgcca
    10621 gaaaaagagc ataaaagagc acacaaaaag agacaaaaac aagatctact aaataaaggt
    10681 tctttatttg atagacttag ttgttcatgt ctcccttacc tccgagtatt agtatcttta
    10741 tactatacct ttaaggaaat tttgtcaaca gaaaactgga aattctatgc tctaaaatcc
    10801 aaaaacaggt ccatagagac ttagtacgtg tttgacatgc tcgtaatgga ttttatcata
    10861 gtttcgccaa gcagtgcgtg cttgattgtt tagttttagg ctacccagtc caatcagagg
    10921 actggtgggt tggtaaaatt tctcaagatc aattaagata ctgttgttca acttttccgt
    10981 ttttttgagc tcttgcatag tagtgtttag cagctcttgt agtcgaatat catctgccgt
    11041 accctgttta cagttttggt agcttttatt taacttggat aggagttgaa tgagtttctt
    11101 gtcttccatg tgagcatcct ccttgatctg atgtactctt tccctatagt atagcactaa
    11161 aaaacaaaac tatcatggta aatatgttaa aattttgaat tttaattaca aagtttggtg
    11221 tgctgatttg tagccttttc atggtataat caagagagta aatctttatg gaggaagtat
    11281 gaaattaaat attcaagaaa ttcgtaagca gtctgaaggt ttgaactttg aacaaacgtt
    11341 agatttagtt gatgacctgc gtgcacgtaa tcaagaaatt ttagatgtaa aagatatcct
    11401 tgcagttggg aaagtacaat atgaagaccg tatgtatttc ttagattatc aactatctta
    11461 taccattgtt cttgcttcga gtcgcagtat ggagccagtt gagttagttg aatcttatcc
    11521 agtcacggaa gttttcatgg aaggcgcaac taaccatcta aatcaagaaa ttttagatga
    11581 tgacttggtc ttgcccatcg aaaatggggc gcttgacctt gctgagagtg tatcagacaa
    11641 tatcctgcta aacattccta tcaaggtctt gacggctgaa gaagaagctg gtcaaggatt
    11701 tgtctcagga aatgactggc aaatcatgac agaggaagaa taccaagctc aacaagcagt
    11761 aaagaaagaa gaaaacagtc cttttgctag cttacaagga ctatttgacg gagatgaata
    11821 atggtcttgt caaaaaaacg agcacgaaag gtgctagaag aaatcattgc cctcttccca
    11881 gatgccaagc ctagtcttga ttttaccaat cattttgaac tcctggttgc ggtcatgtta
    11941 tcagcccaga cgacagatgc agcggtaaat aaggccacac caggtctctt tgttgccttt
    12001 ccaacacccc aagctatgtc tgtagcgaca gagagtgaga ttgcttcaca tatttctcgc
    12061 ttgggattgt atcggaataa agctaaattc cttaaaaaat gtgcccaaca gttactagac
    12121 gattttgatg gtcaagtccc tcagacacgt gaagaattgg agagtttggc aggtgttggt
    12181 cgcaagacag ccaatgttgt catgagtgta ggatttggga ttccagcctt tgcagtggat
    12241 actcatgtgg agcgtatttg caaacaccac gatattgtca aaaaatcagc gacgccactt
    12301 gaggtggaaa agcgggtcat ggatatcttg ccgcctgagc agtggttagc tgcccatcag
    12361 gccatgattt attttggaag agccatttgc catccaaaaa atccagagtg tgatcagtac
    12421 ccacaattat atgattttag caatttgtaa gatgctacat tgaaactttt tgcttgattt
    12481 taagcatgct ttgtgctata gtaaatgata actaaatatt cctttaaacc tgtcccgtga
    12541 ggcaggcaag gagcgcgata aagtagaagg gtgaagtcta tactgtgtgc agtagggctc
    12601 gcttggctat aaatttcaag tctccttgtt taaggagact tttctttttg gaggtttgtc
    12661 atgaaaacaa aagaagttgt agacgaattg accgtcaaac gagcgattac gcgtattact
    12721 tatgagatta tcgaacgcaa caaagatttg aataagatcg tcttggctgg tattaaaact
    12781 cgtggtgtct ttattgccca ccgaatccaa gaacgtttga agcagttaga aaatctttca
    12841 gttcctgttg tggaattgga tactaaacct ttccgtgatg atgttaaaag tggagaagat
    12901 acttctttgg tttctgtcga tgtgacagac cgcgaagtta tcttggtgga tgatgtgctc
    12961 tatacaggtc gtaccatccg tgctgctatt gataatattg tcggtcatgg tcgtcctgcg
    13021 cgtgtgagtt tagcagttct agtcgatcgt ggacatagag aattgccaat ccgtccagat
    13081 tacgttggaa aaaatatccc aaccagtcgt tctgaagaaa tcatcgtaga gatgacagaa
    13141 cttgatgacc aagacagagt tctgattact gaagaagctt agaaagttaa aggagtagcc
    13201 atgtcagaaa atcaacaagc attgaaccat gtggtgtcca tggaagacct cactgtcgat
    13261 caagtaatga aattgatcaa gcgaggaatt gagtttaaaa atggagccca gcttccctat
    13321 gaagaccatc cgattgtttc caatctcttc tttgaggatt ctacacggac acataagtcc
    13381 tttgaagtcg cagagattaa acttggattg gaacgacttg actttgatgt gaagactagc
    13441 tcggttaata agggtgagac actttatgac accattttga ctctgtctgc tttaggagtg
    13501 gatgtctgtg tgattcgcca tccagaggtc gactactata gagaattgat tgctagtcca
    13561 acgattacga cttccatcat caatggtgga gatggttcgg gccaacaccc tagccagagc
    13621 ttgcttgatt tgatgaccat ttatgaggaa tttggccact ttgagggtct taaagttgct
    13681 attgcaggtg acttggacca ctcacgcgtt gccaaatcca atatgcagat tttgaaacgc
    13741 ttgggagctg aactcttttt cgctggacct gaggaatgga gaagtcaaga gtttgcagac
    13801 tatggacagt ttgtaaccat tgatgaaatc attgatcagg ttgatgtcat gatgtttctc
    13861 cgtgtgcaac acgaacgcca tgatagtgga gctgtatttt caaaagaaga ctaccatgcc
    13921 caacatggct tgactcaaga acgttacgat cgtttgaaag aaacagcaat cctcatgcac
    13981 ccagctccaa tcaatcgtga tgtagaaata gcagaccact tggttgaagc accaaaatca
    14041 cggattgtcc aacaaatgac caatggtgtc tttgttcgaa tggcaatctt agaatccgta
    14101 ctagcgagta gaaacgccaa ctaaaacaca agacgttaaa agacgccagt gagcgtccac
    14161 gagaggtgaa aatatgacaa aaagaattct agtattagaa gatggcacag tttttgaagg
    14221 caaggccttc ggagcagata ttgatgtaac aggcgaaatc gtctttaata cagggatgac
    14281 cggctaccaa gaatccatta cagaccagtc ttataatgga caaatcttga cctttactta
    14341 tcctttggta ggaaattatg gtattaatcg tgatgattat gaatccatta ttccaacttg
    14401 taagggagtc gttgtttttg aagaagcgcg tagagctagc aactggcgca accaaatgac
    14461 cttggatgaa tttttgaaag ccaagaaaat tccagggatt tcaggaattg atacgcgtgc
    14521 tcttaccaag attatccgta agcatggtac tatgcgtgca accttgaccc atgttgggga
    14581 cagtatggac catgtgacgg accagctcca agcaacagtt ttaccgacag acaatatcaa
    14641 acaggtttct actaaaacgt cttatccagc tccaggagtt ggtttgagcg tggtgctagt
    14701 ggactttggt ctcaagcact caatcctacg tgaactttct aaacgcaact gtaacgtgac
    14761 agttgttcct tattcgacaa cggctgaaga aattctccat ctcaatcctg acggagttat
    14821 gttgtcaaat ggtccaggta acccagaaga cgttccacaa gcactcgaca tgattcgcgg
    14881 tgtacaagga aaaattccaa tctttggtat ttgtatggga caccaactct ttgcaatggc
    14941 aaacggggct aagacctata agatgaagtt tggccaccgt ggattcaacc atgcggtacg
    15001 tgaaatcgca acaggacgag tagacttcac cagccagaac catggttatg cagtcagccg
    15061 cgaggatttg ccagagcact tgattattac ccacgaagaa atcaatgaca agtcagtgga
    15121 aggtgtgcgc cacagatacc aacctgcttt ctctgttcaa taccacccag atgcagctcc
    15181 tgggccacac gacgctagct acctatttga cgagtttatc gagatgatgg aagtttttaa
    15241 acaatcaaac taagaacgag taaaagctcg tcggagaatt tatgtaactg aacacggacg
    15301 gaaagctcag aatgtagaga aaccaacttg gattgtcaat ccttccttga tttcctaaaa
    15361 ttcaatcgct ttctattcgt cctttgtatc ttatttaatg tcgccctgag aggcgacgaa
    15421 tagaaaggag aagatacatt gttcgcggaa gtgaacacgc cctaagaact gtgtgaaaaa
    15481 gataaacatc gtcttgacgc tgaaggcgtc tgcgccgagt ttcctatttt cactgtgttc
    15541 tttacgggct ttgtatcata aactatgcct aaacgtactg atattcaaaa aattatggtg
    15601 attggttctg gtccgattat tattggtcag gctgctgagt ttgactacgc tgggactcag
    15661 gcttgcttgt cgttgaaaga ggaaggttat gaggttgttt tggttaactc aaatcctgca
    15721 accatcatga cggacaagga aattgctgac aaggtttaca ttgaaccgat tacacttgag
    15781 tttgtgacac gtattcttcg taaggaaggt ccagatgcct tgctaccaac actcggtggt
    15841 cagacaggtc tcaacatggc catggaatta tctaaaaatg gtatcctaga cgagcttggc
    15901 gttgaacttc tgggtaccaa attatctgcc attgaccaag cggaggaccg tgacctcttt
    15961 aaacaattga tggaagagct tgagcaaccc attccagaat ctgaaattgt taacacagta
    16021 gaagaagcag ttgcctttgc agcgacaatt ggctacccag tcatcgttcg tccagccttt
    16081 acccttggtg gtactggtgg tggtatgtgt gccaacgaaa aagaattgcg tgaaatcact
    16141 gaaaatggtt tgaaattgtc acctgttacc caatgtttga ttgagcgttc gattgccggt
    16201 ttcaaggaaa ttgaatacga agtgatgcgt gactcagctg acaatgcctt ggttgtttgt
    16261 aacatggaaa actttgaccc agttgggatt cacacagggg attccattgt atttgcccct
    16321 gcgcaaacca tgtcagacta tgaaaaccaa atgcttcgtg atgcgagctt gagcattatt
    16381 cgcgccctca agattgaagg tggatgtaat gttcagctag ctcttgatcc taacagcttc
    16441 aagtattatg ttatcgaagt aaaccctcgt gtatcacgtt cgtcagccct tgcttctaaa
    16501 gcgacaggat atccgattgc taagttggct gccaagattg cagtaggttt gaccttggat
    16561 gaggttataa acccagttac aggttcaacc tatgccatgt ttgaaccagc ccttgactac
    16621 gtagttgcta agattccacg tttcccattt gacaagtttg aaaagggtga acgccgtctc
    16681 ggtacacaaa tgaaggcgac tggagaagtc atggcaatcg gtcgtaacat cgaggaatct
    16741 cttctcaagg cctgccgatc ccttgaaatt ggggtgcacc acaatgaagt acctgaactt
    16801 gcagcagttt ctgatgatgc cttgattgaa aaggttgtca aagcccaaga tgaccgtctc
    16861 ttctacgtat cagaagcaat tcgtcgtggc tacacacctg aagaaattgc agagcttaca
    16921 aaaattgata tcttctatct tgataaactc ttgcatatct ttgaaattga gcaagaattg
    16981 ggtgctcatc cacaagatct agaagttttg aaaacagcca aacttaatgg tttttcagac
    17041 cgtaagattg ctgaactctg gggaacgact gatgataaag ttcgccaact tcgtttggaa
    17101 aacaagattg tcccagtcta taagatggta gatacttgtg cggcagagtt cgactccgaa
    17161 acgccatatt tctattcaac ctatggttgg gaaaatgaat ctatcagatc tgataaagaa
    17221 tctgtacttg tcctaggttc aggtccaatc cgtatcggtc aaggggttga gtttgactac
    17281 gcaaccgttc actctgttaa ggctatccag gcagctggtt atgaagctat catcatgaac
    17341 tcaaacccag agaccgtttc aacagacttc tctgtatcag ataagcttta ctttgagcca
    17401 ttgacattcg aagatgttat gaacgtcatt gacttggaac aaccaaaggg agttatcgtt
    17461 cagttcggtg gtcaaacagc catcaacctt gcggagccat tggcaaaagc aggtgtgacc
    17521 atccttggta cacaggttgc tgacctagac cgtgccgaag accgtgacct cttcgagcaa
    17581 gctctcaaag aattggatat tccacagcca ccaggacaaa cggctaccaa tgaagaagaa
    17641 gcagctcttg cagctcgtaa gattggcttc ccagttcttg tccgcccatc ttatgtactt
    17701 ggtggacgtg ccatggaaat cgttgaaaac gaagaagacc ttcgttctta catgcgaact
    17761 gctgttaagg ctagtccaga ccatccagtt cttgtcgact cttatatcgt tgggcaagag
    17821 tgcgaagttg atgccatttc agatggtaaa aatgtactca ttcctggtat catggagcat
    17881 atcgaacgtg ctggtgtcca ctcaggtgac tcaatggctg tttacccacc acaaaccttg
    17941 tcgcaaaagg ttcaggagac catcgcagac tacaccaaac gcttagcaat cggtcttcac
    18001 tgccttggaa tgatgaacat ccagtttgtt atcaaggatg aaaaagtcta cgttattgag
    18061 gtcaatccac gtgccagccg taccgttcca ttcctatcta aggtaaccaa tattcctatg
    18121 gctcaagtag cgaccaagct cattcttggt caaagtttat cagaacttgg ctaccaaaat
    18181 ggactttacc ctgaaagcac ccgtgttcat atcaaggcac ctgtcttctc ctttaccaaa
    18241 ctagctaagg tagacagctt gctcggtcct gaaatgaagt caacaggtga agttatgggt
    18301 tctgatgcta ctttggaaaa agctctctat aaagcctttg aagcttctta tctacacttg
    18361 ccaacttttg gtaatgtcgt ctttaccatc gctgatgatg ccaaagaaga agccttgaac
    18421 ttggctcgtc gtttccaaaa cattggttat ggaatccttg cgacagaagg gacagcagcc
    18481 ttctttgcta gtcatggctt gcaagcccaa cctgttggta agattggtga tgacgataag
    18541 gatatcccaa gttttgtacg caaaggaaga attcaagcta tcattaacac agtaggaaca
    18601 aaacgaactg ctgacgaaga tggtgagcaa attcgtcgtt cagccattga acacggggtg
    18661 cccctcttca cagcgctaga tacagctaat gccatgctta aggtacttga aagtcgtagc
    18721 tttgttacag aagcgattta aatagaaaaa taaggtatag cagactgaat ttagaacagt
    18781 acacagggat actaaaatat ttctagaaat taatttgaat tttctaattg atttgttcgc
    18841 atcttatttc aatctactat agaaaaaagt ctttaaaatt ataaaacgca tcatatcaag
    18901 atttttcaaa aa
//
LOCUS       DBERDK010000038        18905 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.038, whole genome shotgun sequence.
ACCESSION   DBERDK010000038 DBERDK010000000
VERSION     DBERDK010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 18905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18905
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.038"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(1..499)
                     /locus_tag="ACL73L_001286"
                     /pseudo
     CDS             complement(1..499)
                     /locus_tag="ACL73L_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
     gene            complement(536..664)
                     /locus_tag="ACL73L_001287"
                     /pseudo
     CDS             complement(536..664)
                     /locus_tag="ACL73L_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CD1845 family protein"
     gene            complement(726..1595)
                     /gene="rsmI"
                     /locus_tag="ACL73L_001288"
     CDS             complement(726..1595)
                     /gene="rsmI"
                     /locus_tag="ACL73L_001288"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HGS0772260.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQTLKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKIPDLIGFLKAGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGASAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFGL
                     KKDYPETQIFYESPHRVADTLENMLEVYGDRSVVLVRELTKIYEEYQRGTISELLESI
                     AETPLKGECLLIVEGASQGVEEKDEEDLFVEIQTRIQQGVKKNQAIKEVAKIYQWNKS
                     QLYAAYHDWEEKQ"
     gene            complement(1598..1915)
                     /gene="yabA"
                     /locus_tag="ACL73L_001289"
     CDS             complement(1598..1915)
                     /gene="yabA"
                     /locus_tag="ACL73L_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HGS0772261.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            complement(1955..2845)
                     /locus_tag="ACL73L_001290"
     CDS             complement(1955..2845)
                     /locus_tag="ACL73L_001290"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HGS0772262.1"
                     /translation="MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFGSLEIAQF
                     LAKSLFCTDKVGVLPCEKCRSCKLIEQEEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLANQASFWTLVDESERLLT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARK
                     MWQANVSFQNAMEYLVLKEI"
     gene            complement(2842..3480)
                     /gene="tmk"
                     /locus_tag="ACL73L_001291"
     CDS             complement(2842..3480)
                     /gene="tmk"
                     /locus_tag="ACL73L_001291"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HGS0772263.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLPILEEKGVEVLTTREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLDLHKKVRQGYLSLLDKEGNRIVKIDASLPLEQVVETTKAVLFDGMGLAK"
     gene            complement(3700..4497)
                     /gene="proC"
                     /locus_tag="ACL73L_001292"
     CDS             complement(3700..4497)
                     /gene="proC"
                     /locus_tag="ACL73L_001292"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HGS0772264.1"
                     /translation="MKIGFIGLGNMGASLAKSVLQTRTSDEILLANRSQAKVDAFIAD
                     FGGQASSNEEMFAEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLTLEKL
                     ASLIPSQHRIIRMMPNTPASIGQGVISYALSPNCRAEDSELFYQLLAKAGLLVELGES
                     LIDAATGLAGCGPAFVYLFIEALADAGVQTGLPREIALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIAGVASLEAHAFRGTVMEAVHQAYKRTQELGK"
     gene            complement(4501..5763)
                     /locus_tag="ACL73L_001293"
     CDS             complement(4501..5763)
                     /locus_tag="ACL73L_001293"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HGS0772265.1"
                     /translation="MVSRQEQFEQVQAVKKSINTASEEVKNQALLAMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAMGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTAL
                     KKGLETTTIHPNVIQLVEDTSRESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADEDKALSIINNAKTSRPSVCNAMEVLLVHENKAASILPRLDQ
                     MLVAERKEAGLEPIQFRLDSKASQFVSGQAAETQDFDTEFLDYVLAVKVVSSLEEAVA
                     HIESHSTHHSDAIVTENAEAAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVAGDGQIRE"
     gene            complement(5773..6882)
                     /gene="proB"
                     /locus_tag="ACL73L_001294"
     CDS             complement(5773..6882)
                     /gene="proB"
                     /locus_tag="ACL73L_001294"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HGS0772266.1"
                     /translation="MKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAMLHEAGHEL
                     ILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQIVSAQILL
                     TQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDELKVGDNDTLSAQVAAMVQ
                     ADLLVFLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTGGMLTKIK
                     AATIATESGVPVYICSSLKSDSMIEAAEETEDGSYFVAQEKGLRTQKQWLAFYAQSQG
                     SIWVDKGAAEALSQHGKSLLLSGIVEAEGAFSYGDIVTVFDKESGKSLGKGRVQFGAS
                     ALEDMLRSQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            complement(6983..8866)
                     /gene="cbpE"
                     /locus_tag="ACL73L_001295"
                     /gene_synonym="pce"
     CDS             complement(6983..8866)
                     /gene="cbpE"
                     /locus_tag="ACL73L_001295"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HGS0772267.1"
                     /translation="MKKKLTSLALVGAFLGLSWYGNVQAQESSGNKIHFINVQEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWREGIETSYKHVLTDRVFRRLKELGVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDSRITNSERLWDNLYGYDKVLQT
                     AAEKGVSVIQNITQGDAHFQFGDMDIQLYNYENETDSSGELKKIWDDNSNSLISVVKV
                     NGKKIYLGGDLDNVHGAEDKYGPLIGKVDLMKFNHHHDTNKSNTKDFIKNLSPSLIVQ
                     TSDSLPWKNGVDSEYVNWLKERGIERINAASKDYDATVFDIRKDGFVNISTSYKPIPS
                     FQAGWHKSAYGNWWYQAPDSTGEYAVGWNEIEGEWYYFNQTGILLQNQWKKWNNHWFY
                     LTDSGASAKNWKKIAGIWYYFNKENQMEIGWVQDKEQWYYLDVDGSMKTGWLQYMGQW
                     YYFAPSGEMKMGWVKDKETWYYMDSTGVMKTGEIEVAGQHYYLEDSGAMKQGWHKKAN
                     DWYFYKTDGSRAVGWIKDKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVSIEKS
                     ATIKTTSHSEIKESKEVVKKDLENKETSQHESVTNFSTSQDLTSSTSQSSETSVNKSE
                     SEQ"
     gene            complement(8869..9756)
                     /locus_tag="ACL73L_001296"
     CDS             complement(8869..9756)
                     /locus_tag="ACL73L_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HGS0772268.1"
                     /translation="MEIKIETGGLRLDKALSDLSELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDLPLEIVYQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHLVLAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKPAVTRFHVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHPVAGDEVYGPRKTLKGHGQFLHAKTLGFTHPRTGKTL
                     EFKADIPEIFKETLERLRK"
     gene            complement(9746..10207)
                     /gene="lspA"
                     /locus_tag="ACL73L_001297"
     CDS             complement(9746..10207)
                     /gene="lspA"
                     /locus_tag="ACL73L_001297"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HGS0772269.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMVLGLTLIIAGGLG
                     NFIDRVSQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAMLKEEINGN"
     gene            complement(10204..11112)
                     /locus_tag="ACL73L_001298"
     CDS             complement(10204..11112)
                     /locus_tag="ACL73L_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGS0772270.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLCEGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            11105..11302
                     /locus_tag="ACL73L_001299"
     CDS             11105..11302
                     /locus_tag="ACL73L_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HGS0772271.1"
                     /translation="MFIFYPQLLYLFIIPYFRSFPKRKKEHPILLNYLHYLPFSRQSY
                     FQNQFKKISGYTPLQYRKEKR"
     gene            complement(11354..12025)
                     /locus_tag="ACL73L_001300"
     CDS             complement(11354..12025)
                     /locus_tag="ACL73L_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HGS0772272.1"
                     /translation="MKKRAIQILLALSLIFYKSTWFWRVFNHLAKPYLPASREFFQIL
                     LLMESGVLFLAVIYLLVFAGKKFFHFKWQLRYFIYLLLGYIISYMSDFLFSYFISLSS
                     NQISLNETVEMMGRQEFPYFLLIVCFIAPIAEELIYRGVLMTTFFKNSPWYGDVLLSA
                     IIFGYIHINFALTPLAFFIYASGGLILALLYRMTKNLYYPILVHILINITAFWDVWLL
                     LFSGS"
     gene            complement(12079..12300)
                     /locus_tag="ACL73L_001301"
     CDS             complement(12079..12300)
                     /locus_tag="ACL73L_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0924 family bacteriocin-like peptide"
                     /protein_id="HGS0772273.1"
                     /translation="MILMTKNINLTNEELELIQGGADSYGKNPNGRYDWEIEPVLTLP
                     VHGFCPRGTYDLGYIGGGNHLCKGSAVRF"
     gene            12572..13381
                     /locus_tag="ACL73L_001302"
     CDS             12572..13381
                     /locus_tag="ACL73L_001302"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HGS0772274.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPQAHIAAIHQAIEKGVKLVLCTGR
                     PLFGVLPYYKKLGLDLQNEYVIVNNGCSTHQTSDWSLVDWQELSPADIEYLYDLAEKS
                     DVQLTLFDESHYFVLGGKPNQVIENDAKLVFSDLTEISLEEATSGKFRMFQGMFLGTK
                     EQTDDFEQRFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASDYVKSLADAVTSSNEEDGVARAIEKYIL"
     gene            complement(13562..14437)
                     /gene="aguB"
                     /locus_tag="ACL73L_001303"
     CDS             complement(13562..14437)
                     /gene="aguB"
                     /locus_tag="ACL73L_001303"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0050126 - N-carbamoylputrescine amidase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HGS0772275.1"
                     /translation="MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKVIAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGEVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYDLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(14447..15532)
                     /gene="aguA"
                     /locus_tag="ACL73L_001304"
     CDS             complement(14447..15532)
                     /gene="aguA"
                     /locus_tag="ACL73L_001304"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047632 - agmatine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HGS0772276.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKAAKRAF
                     TQIIETIAEGERVYLLVEQAYLSEAQSYLGDKVVYLDIPTNDAWARDTGPTILVNDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEALEKPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVA
                     AFVGPAELVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPAVRQVVTE
                     EDLPGYIYEEGEEERYAGERLAASYVNFYIANKAVLVPQFEDVNDQVALDILSKCFPD
                     RKVVGIPARDILLGGGNIHCITQQIPE"
     gene            complement(15529..16656)
                     /gene="nspC"
                     /locus_tag="ACL73L_001305"
     CDS             complement(15529..16656)
                     /gene="nspC"
                     /locus_tag="ACL73L_001305"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0045312 - nor-spermidine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HGS0772277.1"
                     /translation="MKLEQVPTPAYVIDLAKLEANCRILQYVQEEAGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDEDMEELLGITDHIVF
                     NSERQLRKHGSRCREAGVSVGLRLNPQCSTQGDHALYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGLHFHTLCEQGADDLQTTLKAVEEQFGPYLHEVKWLNMGGGHHITREGYDVDLL
                     ISEIKRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPPLRNGFESQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            complement(16656..17915)
                     /locus_tag="ACL73L_001306"
     CDS             complement(16656..17915)
                     /locus_tag="ACL73L_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HGS0772278.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTRIETAALDADKVEEVIALIESYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGKWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDAINFNGQDIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV
                     KDGVEKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLPWVVVENPQMVD"
     gene            complement(17912..18772)
                     /gene="speE"
                     /locus_tag="ACL73L_001307"
     CDS             complement(17912..18772)
                     /gene="speE"
                     /locus_tag="ACL73L_001307"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HGS0772279.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPF
                     GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQAFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDKEGWKKRQLFTEYYTANLHVGAFMLPKYVE
                     DILEEEEGKK"
BASE COUNT         5470 a         4420 c         3407 g         5608 t
ORIGIN      
        1 ttttctgatt ttagttctga aagttccaaa aggtgcaacc gtatttacat gaataaactt
       61 atacacttcc caagtcgctg ttttagtcgc ttcatcagcc cactttctca tatgcggttc
      121 aaataattct tcctcactca acgaatcaat cattgcagag atagaattaa tattttcatt
      181 taattttgct ttcaactctt gcagagataa atgagcgtag gtatctgtga accactgata
      241 taaattcacc aagttgattc catttaaatt tatccgatgg tgtttttact tgaagcccct
      301 ttctttcatc ttcttcccat ttaagaacca agttggtcca gccaacctga taagcaaggt
      361 tttctgctgg agttctatca acttcatcaa ctctcttatc ttttaaattt tctggaatat
      421 tatcaaattc tgaaatatat ttctcaaatg ttttctctat ctcagctttt agttcttctt
      481 tattttcgta tgttcgcaaa aaatcacctt ctttctgatt tatcaatcct ttgatttaaa
      541 atgaccttat ttttctattt ataccttcta ggaacactat aactgctgct ccaaccatcg
      601 gtattccgta tgggcttaat aaaaaactta ataccaaagc ttctatacca agtgatacat
      661 ctttatatta tcatcactaa ttaagttaaa cagaaacaga cagaattatt acatcctgtc
      721 tccctttatt gtttttcttc ccagtcgtgg taggcagcgt agagctgact tttattccac
      781 tggtaaatct tagcgacttc cttgatagct tggtttttct tcacaccttg ctggatgcgg
      841 gtttgaattt ctacgaacaa gtcttcctcg tccttttcct ccacaccctg actggcaccc
      901 tcaacaatga gaagacattc gcccttgagt ggcgtttcag caatgctttc taataactca
      961 gagatagtac ctcgttggta ttcttcatag attttggtca attccctgac caagacaact
     1021 gagcggtcac cgtagacttc taacatattt tccaacgtgt ctgctacacg atgaggtgat
     1081 tcataaaaaa tctgtgtttc aggataatct tttttcaagc caaaaaattg cttctgctga
     1141 cctgattttc tcggtaaaaa accgtaaaag atatgtggct gtggcgctaa accactggca
     1201 atcaaggcag aaattcctgc agaggcacct ggaactgtca caactgcaat ttcttcctca
     1261 atagctgcct taactaaatc atgaccaggg tctgaaatgc taggcaaacc ggcatcagag
     1321 acctgagcaa tactttgccc tgctttcaag aaaccaatca aatcaggaat tttttccttg
     1381 gcattgtgct catgaaaact gatctgctta gtggaaatgt caaaatgctt gagcaaaaga
     1441 cctgtattgc gcgtatcctc agcagcaatc caatctactt ctttcaaggt ctggatagct
     1501 cgaaaggtca tatcatctag attgccaatc ggcgttgcca ctagatacag cttgccatag
     1561 ggagactgcc ccttaaaact tttttgaatc tgcatgccta ctccctgtat agcaactcgt
     1621 cacaaaacat acattcctcg tcctgctctc gacgttgtcc ataaaaatca ttacatacgt
     1681 gaaatccatc acggtaaatg cgacggacac tttcacgaac atgcttggcc ttgacaggag
     1741 catctgcttc cacctcaccc aagcgttctc gcaacttact attttccaag cgaagagctg
     1801 tattttcctc taccaggctc ttgagatttt tcttgatggc ttccacatcg gctaaggtta
     1861 ccaataattg ttgggaaaaa tcatccagcg cgtcaaataa ttcttttttg tccataaaac
     1921 agccctttcc tttctttatc attcattttt gagtttatat ttctttcaag accagatatt
     1981 ccatggcatt ttgaaagctg acattagctt gccacatttt tctagcttct agtaaatctt
     2041 gtagaatcac tcttactctt acctgcaaga ggtcctgccc acagagaact tcaagaatcc
     2101 gtaaaacctg atcctgtttt tccttatcat ctgccaagtt ggctaatttg gcaacctgta
     2161 gataactttc ttttttctta gctactaacc aagtcagcag gcgttcactt tcatcgacca
     2221 aggtccaaaa acttgcctga ttagccaact tttctgcttc agctcgcgat tgactaaact
     2281 tagctaaaag agtcgctttt ttcttaacaa gtcccatttg ttctaagagt aagataagtt
     2341 tttcttcttg ctttttaaag tggaagatct gagtccgact tcggattgtc ggtaacatct
     2401 tttcctcatc gctagtcaag aagaaaatat aaacttcact ctggggttct tcgatgacct
     2461 tgagcagaga attggctgcg ttgggatgca ttttatccgc ttgctcgatg ataaagacct
     2521 gttgctggct ttcaatccct gcttgagaaa actgtcccac caattcccga atgcgttctg
     2581 tcttgatgac ctgattgact ggcttaatca aggtgacatc tggaaactct tcctgttcaa
     2641 tcagcttgca acttcggcat ttctcacatg gtaagacgcc aactttatcc gtacaaaaga
     2701 ggctcttagc taaaaattgc gctatttcca agcttccaaa gaaacctgaa aagagatagg
     2761 cgtgattgag ctggtcttgt tctaagatac ggacaaaacg gtcaaactga gctggttgcc
     2821 aagcctttag ttgatcttgt ttcatttggc caagcccatt ccgtcaaaca agacagcctt
     2881 ggtagtttcc acaacttgct ccaaagggag actagcatca atcttgacaa tgcgatttcc
     2941 ctctttatcc agaagagaaa ggtagccttg acgaactttt ttatgcaagt ccaacccttc
     3001 caaatccaaa cgattaacct cgcggtcact attagcagca atacgagcca gcccttcttc
     3061 cacctcgatg tcaaaataga gtgtcaaatc gggtttgagg ccatctgtcg caaactgatt
     3121 gagccagtca atggcttcaa tatctaagcc acgaccaaat ccctgatagg caacagaact
     3181 atcgataaaa cgatccatga tgaccaactt gccagcttca agtgctggaa gaactttttc
     3241 caccaaatgc tgtctgcgac tggcaatata gagaagtagc tctgttttag catccatctg
     3301 agtatgactt ggatccaaaa tcacttcccg aatcttctcc ccaatcaaga ctccgccagg
     3361 ttcacgggtc gtcaacacct ctactccttt ttcctctaaa attggtagca gagcctctaa
     3421 aacactggtc ttgcctgctc cctctggtcc ctcaagagag actaaaaatc cttttgacat
     3481 gtctaactca tttctttttt actacttcta ttctatcaaa aaaatagggg tttgtgacaa
     3541 tatttttgtc ttctcatttc atactcaatg aaaatcaaag agcaaactag gaagctagcc
     3601 gcaggctgta cttgagtacg gcaaggtgaa gctgacgcag tttgaatttg attttcgaag
     3661 agtatcagcc accataaaag aggcagtcaa agctacctct tatttaccta gttcttgtgt
     3721 tcgtttatag gcttgatgaa ctgcctccat gactgtgcct cgaaaagcat gcgcttctag
     3781 gcttgctacg ccagcaatag tcgaaccgcc tggactacaa acttgatctt tcaaaactcc
     3841 aggatgttgc tgactttcaa ggaccaattg cccagctcct accacagttt gtgctgccat
     3901 tttcaatgct atttctcgtg gtaatcctgt ctgaacacct gcatctgcca aggcctcgat
     3961 aaaaagatag acaaaggctg gtccacaacc tgcaagacct gtcgctgcat cgattaaact
     4021 ttctcctagt tcaaccaaga gaccagcctt ggctaaaagc tgataaaaga gctcactgtc
     4081 ctcagccctg caattaggag acaaggcata actaatcact ccttgcccga tagaagcagg
     4141 ggtattaggc atcatacgaa taattcggtg ttgacttggg ataagacttg ctagtttttc
     4201 taaggtcaat ccagctgcca tcgaaatcaa aagaagactt tctctttttt caaggatggt
     4261 ctggtattga gaaagcagtt cagaaaactg agcaggctta actcctagaa aaatcacatc
     4321 tgcttctgca aacatttctt cattgctgga agcctgacca ccaaagtctg caatgaaagc
     4381 atctacctta gcttgactac gattggcaag gagaatctca tctgacgtcc tagtctgcaa
     4441 gacagatttt gccaagctag cacccatatt ccccaaaccg ataaatccaa tcttcatctc
     4501 ttactccctt atctgcccat caccggcaac cacatacttg tagctggtca actctttcaa
     4561 gcccatggga ccacgcgcgt gcaatttctg agtagaaatc cccatttcac aaccaagacc
     4621 aaattgtcct ccatctgtga aacgagttga ggcattaaca tacaccgctg cagagtccac
     4681 ttgatctgta aagtatgctg cagcttcagc attttccgtc acaatagcat ccgaatgatg
     4741 ggtgctgtgg gattcaatgt gcgcaaccgc ttcttctaaa ctgctcacaa ccttaacagc
     4801 aaggacatag tctaaaaact cggtgtcaaa gtcttgggtc tcagctgctt gacctgaaac
     4861 aaactggctt gctttgctat ccaagcggaa ttgaattggt tccagtccgg cttccttacg
     4921 ctctgcaacc agcatttgat ccaagcgagg aaggatgctt gctgccttgt tttcatgaac
     4981 cagcagaacc tccatggcat tacaaacaga aggacgactg gttttagcat tgttgatgat
     5041 agacagcgcc ttgtcttcgt ctgcatcctt atccacatag acatggacaa tcccagtccc
     5101 tgtctcgata acaggtacaa tcgcattctc aaccactgca ttgatcaagc cagctcctcc
     5161 acgaggaatg agaaggtcta gatagccctt ggccttcatc atagcataac tactttcacg
     5221 gctagtatcc tccaccagtt gaatcacatt tggatgaata gtagtcgtct ccaagccctt
     5281 cttcaaggct gtgacaatgg catgggttgt ttgataggca tccttaccac tacgaagaac
     5341 aaccgcattt ccactcttaa gagtcaaagc agccgcatca gacgtcacat ttggacggct
     5401 ttcatagata ataccgatga cacccatagc tacacgtttt tttgtgataa ccaaaccatt
     5461 ttcaagctga cttgtttcta aaacttcacc gattggatct ggtaaggcaa ccacttcacg
     5521 aattcctctt gccatcgctt ctatacgatc tgcatccaaa taaagacgat ccaacatcac
     5581 atctgagatt ttccccttag ccgctgccat atcgagggca ttagccgcta aaatttcctc
     5641 agtagcagcc actaagtgat cagccatggc tagcaaggct tggtttttca cttcttcact
     5701 agctgtgtta atcgattttt taacagcctg tacctgttca aattgttctt gtctactcac
     5761 catagtttac ctctaaaatt ctgtaaaaag tagttggatt tcaggagtaa tggaaatcca
     5821 gtcgtcacgg taaatcaaga cacccttggc tttttgagaa cgcaacatat cctccaaagc
     5881 agatgctcca aattgcacgc gtccttttcc aagtgatttt ccactttcct tgtcaaatac
     5941 tgtcacgata tcaccgtaag aaaaggctcc ttctgcttca acgataccag ataagagaag
     6001 actctttcca tgttgagaga gagcttccgc agccccttta tcaacccaaa tagaaccttg
     6061 actctgagca tagaaggcaa gccattgttt ctgggtacga agccccttct cttgagcaac
     6121 aaagtaagaa ccatcctcgg tctcctctgc cgcctcaatc atggaatctg atttcaagga
     6181 tgagcagata taaacaggaa ctcctgattc cgtcgcgata gttgcagcct tgattttggt
     6241 taacataccc ccagttccgt ttgacgaacc agctccacca gccatatcaa taatctcacg
     6301 attgatggtc tcgattctct ccaagcgttt ggctcttgga tctgaattag gatttccagt
     6361 atagagaccg tccacatctg tcaagaaaac taaaaggtct gcttggacca tcgccgctac
     6421 ttgagcactt agagtgtcat tgtccccaac cttgagctca tcaataacga cactatcatt
     6481 ctcattgatg ataggaattg ccccacggtt gagcaaaacc gacaaagcct gatgggcatt
     6541 tttataacga cgcttatcca caaagtcatc ttgggtcagc aagatttgtg cagaaacgat
     6601 ttgacgcaag agaagattgg tcgtatattc ttccaacaaa agtccctgcc ctaccgctgc
     6661 tgaagcctgt ttatcagcaa ttttagtcgg acgcttttta aatcctaagg ctccaaaacc
     6721 agccgcaatg gcacctgaag acaccaaaat caactcatga ccagcctcgt gcagcatagc
     6781 caactgctgg gtaatatcct ttaccttact acgtgataaa cttccatcct catttgtcag
     6841 agaagaagta cccaccttaa agacaatccg tttgtatttc atagtctcac tccgattatt
     6901 catatttatc cattataaca tgaataacag gaaatagaaa agagttgata ggaaaaagaa
     6961 ggccctaaaa ccttcttttc tactactgtt ctgattccga tttgtttaca ctcgtttcag
     7021 agctttgtga agttgaggat gtcaaatctt gactagttga aaaatttgta acactttcat
     7081 gttgactcgt ttctttattt tcaagatcct ttttcactac ttctttggat tcttttattt
     7141 ctgaatgact tgtagtttta attgtagcag atttctcgat cgaaacatcc actaaccagg
     7201 caccacttga atccacagta taaccttctg gtgtcttacc gttcacaagt aattgaccat
     7261 tttctttcaa gaagtaccat ttatccttgt ccttgatcca acccacagct cgtgaaccgt
     7321 ctgtcttgta gaaataccaa tcatttgcct ttttatgcca gccttgcttc atagctcctg
     7381 aatcttccag ataataatgt tgaccagcaa cttctatctc acctgtcttc atgacaccag
     7441 tagaatccat atagtaccag gtttctttat cttttaccca gcccattttc atttcccctg
     7501 atggagcaaa gtaataccat tgccccatat attgaagcca tcctgtcttc atagaaccat
     7561 caacatccaa ataataccac tgctctttat cttgaaccca accaatttcc atctgatttt
     7621 ctttgttaaa ataataccag attccagcga ttttcttcca atttttagca gaagcaccag
     7681 agtctgtcaa atagaaccaa tgattgttcc attttttcca ttgattctgt aacaagatac
     7741 ccgtttggtt aaagtaatac cattcacctt cgatttcatt ccaaccgaca gcatactctc
     7801 ctgtagaatc aggcgcttga taccaccagt tcccatatgc actcttatgc caaccagctt
     7861 gaaaacttgg aatcggcttg taggatgttg aaatattgac aaaaccgtct tttcgaatat
     7921 caaaaactgt tgcatcatag tctttgctgg ctgcgttgat tctctcaatt cctcgttctt
     7981 tgagccaatt aacatactca ctatcaacac catttttcca aggtagacta tccgaagttt
     8041 gaacaatcaa actcggactc aaatttttaa tgaaatcctt ggtatttgat ttgttggtat
     8101 catggtgatg attaaacttc atcaaatcaa cttttccaat gagaggacca tacttgtctt
     8161 ctgctccatg aacattatct aaatcgcccc caaggtaaat tttcttgcca ttgactttca
     8221 ccacgctaat caaggaattg gaattgtcat cccaaatttt ctttaattca cccgatgaat
     8281 cagtttcatt ttcataatta tagagctgaa tatccatgtc cccaaactga aaatgagcat
     8341 ccccttgtgt gatattttga ataactgaaa cacctttttc tgcagcagtc tgtaaaacct
     8401 tatcatagcc atacagatta tcccatagac gttcagaatt agtaatacga ctatcactat
     8461 atttcttaag atagactcgg tcaactggat aggtagacag taattcatca acatttccaa
     8521 tatgatcact gtgggtatgg gtcaccaaaa taaaatcaag tttttggaca cccaattcct
     8581 tcaaacgacg aaagacacgg tctgttagaa catgcttata agacgtttca attccttctc
     8641 tccatggata acgagaatca cttccatctg ggaaatcata atcttctcct gtatccacca
     8701 tggcaaaatg tccattgctt tcaagaataa tcgcatcact gccaccttct tgaacattga
     8761 taaagtggat tttatttcct gaactttctt gagcctgaac attcccatac catgacaaac
     8821 ctaaaaaagc gcctacaagt gctaaactag ttaatttctt tttcattctt actttctcaa
     8881 tctctccaag gtttccttaa aaatctctgg gatatctgct ttaaattcca aggtcttacc
     8941 tgttctcgga tgagtaaaac ctaaagtctt ggcatgaaga aattgtccat gtcctttcaa
     9001 agtcttgcga ggaccataga cctcatcacc agcgactgga tggccgatat aagccatgtg
     9061 gacacggatt tgatgagtgc gccctgtctc cagttgcaac tctactaagc tataatcgcc
     9121 aaagcgttcc aagacgtgaa aacgcgtcac tgcaggcttc cctttagcag ttacagcctg
     9181 tttcttacgg tctttttcac tccggccaat cggcgcttca attacaccac gatcattagg
     9241 cagatttcca tgaacaatcg cccaatattt gcggagagac tttttatctt tgagttcttg
     9301 ggcaagtact agatgcgcat catcgttttt agcaatcatg agaagacctg acgtatcctt
     9361 atcaatacgg tgaacaatcc ctggacgcag aaccccattg atacccgaca agtccttaat
     9421 atgatacatg agggcattta ctagggttcc actggtatga ccagcactcg ggtgcacaac
     9481 cattccctga ggtttgttaa cgacagccac atcctcatct tggtagacta tttctagcgg
     9541 aagatcctca gccacatact ctaatacctc tggttctggc acatggtaag tgacgacatc
     9601 accctcttgg actgtgtatt tagctttctt gacttgacca ttgaccaaga cctggcctga
     9661 tttaatttgt tcattcgcga gactacgtga taattctgac aaatctgaca aagccttatc
     9721 caaacgcaga ccaccagttt caattttaat ttccatttat ttcctctttt agcattgcaa
     9781 tcaataaaat aatcactcca accgtcagat agctatctgc cacattgaaa attgcaaagt
     9841 tgataaagtc aaggtggaac atatccacaa caaagccctg actgaccctg tcaataaagt
     9901 ttccaagacc acccgcgatt attagagtca aacccaagac catccagaat gagtcctcca
     9961 tgtgtttatg taaataccaa atggcaccta tcacgacaac cagagtaatg acagcgaata
    10021 acagctgctg atcttgtaag atagaaaagg ctgcacctcg attttgcagg taggtcaagc
    10081 taacgaaatt gggaatccag gagcgcactt cacccagtgg aatctgctgg acgatatagg
    10141 atttgaccaa ctgatccagc ccaattaaaa gcagtacaat gactgccact attgctcttt
    10201 ttttcatgat ttcctctttt gatcaaaata ttcttgcatg acttctacga agagagtccc
    10261 agcttgacta agctccactt cttcacgttt aacatagacc atgcggttat ctaggttatc
    10321 cttgagacga ataactgtaa tgccattaac actgtcacta tctaaaaatc cagaacctgt
    10381 cgcataggcg tccgtccgct ccaaaatacc attcaaggtg gcacggtctg tcacattaaa
    10441 catctgtgag ctagcgctgg tatcgacaaa gttctctgaa taataaaggt actcgtcttt
    10501 ctcttgagtg aaacgaaccg ttggtaaatc cgctaaatcc tccatgacta attcctcttt
    10561 ctgggctaaa ggatgaccct cacagagata aatatgggta tggaaaggaa tcaattcgat
    10621 gacctccaga cctaactttt caacccgttg cataatcccc tttttatttt gattgttgag
    10681 gtagataatc ccaatctcac tatgcccttg cgccacttca tctaatattt gaacagtagt
    10741 tgattcaaaa atacggaagt tcttatagtc aggatagcgc tctgaaaagg ccgtaatagt
    10801 tggtggtaag aagtcatagt gctggctagc aacggaaaat tcatcttttt cttcttcagg
    10861 attggcatac tgattttgaa aaatatcaaa tcctttaacc aattcttgcg ctttttcata
    10921 aaattccatc ccacgacggg tcaagaaagt ccctgagctg gtccgacgga aaatcttaaa
    10981 gcccaactct ttttccaaat cacgaacaga aatagacaga ctcggctgac taacatacat
    11041 cttttcagca gcttcacgaa aagtaccact attggcaata gccacaacat agcgtaattg
    11101 ttgaatgttc atcttctacc cccaacttct ttatcttttc attataccat attttagaag
    11161 ttttccaaaa aggaaaaaag aacatcctat tcttcttaac tatcttcact atctgccttt
    11221 ctcacgccaa tcttattttc aaaatcaatt caaaaaaata agtggctaca caccacttca
    11281 gtatcggaaa gaaaaacgtt gactatttgt gaaaaaagaa aattccggaa agttccgaca
    11341 ttattttagt aagctaactt cctgaaaata ggagcaacca cacatcccag aaggcagtga
    11401 tattaatgag aatatgaact agtattggat agtagagatt tttagtcatg cgatacaata
    11461 gagctaaaat aagacctcca ctagcataaa tgaaaaaagc aagaggagtt aaagcaaaat
    11521 tgatatgaat ataaccgaaa ataatagcag aaagcaaaac atctccgtac caaggcgagt
    11581 ttttgaaaaa ggttgtcata agcacaccgc gataaatcaa ttcctcagca ataggggcga
    11641 tgaagcaaac gatgagcaag aaataaggga actcctgtct ccccatcatt tctaccgttt
    11701 cattcaaaga aatctgattt gaagacaggg atatgaaata cgaaaagagg aagtcagaca
    11761 tatatgaaat gatgtagccc agtaaaaggt agatgaagta cctcagctgc cacttgaaat
    11821 gaaaaaattt ttttcctgca aaaaccagta gatagatgac cgctaagaaa agaactccgc
    11881 tctccatcaa aagcagaatc tgaaaaaatt cacgacttgc tggtagatag ggctttgcga
    11941 ggtgattgaa aactctccaa aaccaagttg atttgtaaaa aattaaggac aatgctagta
    12001 aaatttgaat agcccgtttt ttcatattaa attctctgct ttctccactt gtttcttcat
    12061 attactaata aattttactt aaaatctcac agcacttcct ttgcaaagat gattgcctcc
    12121 tccaatatat cctaaatcat aggtgcctct gggacaaaat ccatgaaccg gcagagttaa
    12181 tactggttct atttcccaat cgtacctacc attaggattt ttaccatatg aatctgctcc
    12241 accttgtatc agctctaatt cttcatttgt tagatttata ttttttgtca ttaaaatcat
    12301 gatatatttc ctccattatt cttcagatta gttgagtaat ctgatactca tacctactta
    12361 caaaaaaact attatattaa gttggttttt taatttgtct agttgcaaca ttgacaaaca
    12421 tagtatagca tatctttgcc aaatatcctc ttcaaatcat gaattgtcat caaaacatct
    12481 taaactataa aatcaattag tctcaagctt tctatcaatt tcttctccaa aatatgctat
    12541 aatactacca aaagataaag aaggaagacc tatgattaaa ctactagcct tggatatgga
    12601 tggaaccctc ctcaatgaag ccaaggaaat cccacaggct cacatcgctg ctattcacca
    12661 agccattgaa aaaggtgtca aactggttct ctgtacgggt cgcccccttt tcggtgtcct
    12721 cccctactac aaaaaactgg ggcttgacct ccagaacgaa tatgttattg ttaacaacgg
    12781 ttgttcaact caccagacca gtgattggag tctagttgac tggcaagaac tgagtccagc
    12841 tgacatcgaa tacctctatg accttgctga aaagagtgat gttcagttga cactttttga
    12901 cgagtcacat tattttgttc tcggtggaaa gcccaatcaa gttattgaaa atgatgctaa
    12961 actagtattt tcagacctga ctgaaatttc tcttgaagaa gcgactagtg gaaagttccg
    13021 gatgttccaa ggtatgtttt taggaacaaa agaacaaaca gacgattttg agcagcgttt
    13081 tgctgaagag ctttgccaac gattcagtgg agttcgttcg cagcctgtca tttatgaagc
    13141 aatgccgctt ggaacgacaa aggctactgc tctttcacga ctagctgaga ttttgaagat
    13201 tgattcctca gagattatgg ccatgggcga tgctaataac gatatcgaaa tgctccagtt
    13261 tgcagggctt gggattgcaa tgggaaatgc cagcgattat gtcaaatctc ttgcggatgc
    13321 cgttacctca agcaacgaag aagacggcgt tgcgcgtgct attgagaaat atattctata
    13381 aaaaagaaaa gcaaatagac agaagttact gctatttgct tttttgctaa tattttaaaa
    13441 tacggactaa tcaatagaca agaatagcga atcataaaaa tcgatttact agatgccata
    13501 gtatcttata gttgctaata agaagttagt ctagcagaat gaatctctca tttctcccac
    13561 actaatctgt aatttgtcta tacatttctg gtcttctatc tcgaaacaag ccccagttta
    13621 ggcgttcact tgctcccttg tctaggtcat aagtagctaa cagaacagct tcttcttgtc
    13681 tttcagctcg ttctagaata gctcctgttt catccgtcat aaaggaggaa ccgtagaagt
    13741 caagactgga actctgtccg ccattttcct cactaggagt aacctcctct aaaccataac
    13801 gattggctgc gatgactgga acaatattcg ctgctgcgtg cccttgcata gtacgttgcc
    13861 agtgaccaca actatctgta tccaaaattg gctctgaacc gatagctgta ggataaaaga
    13921 gcaattcagc accattcaat gcaagacagc gcgctgtttc agggaaccat tgatcccaac
    13981 agataccgat accaatctta gcatagcgag tattccagac cttgaaacca gtgttaccag
    14041 gcgtgaaata gaatttttct tgataataat ggtcatctgg tatgtgggtc tttcgataaa
    14101 cgcccagcac ttccccatct gcatcaatga cggcaataga gttatacaag acattaccat
    14161 ctttttcata gaaactgatt ggtaaaacaa cttgtagttc cttagcaatc accttaaaat
    14221 gctgaatggc agtattttcc gctacagatt gggcatactg gtagtagtca tactgacgtt
    14281 cctgacagaa atagggatgt tcaaacaact cgggcaagag aataatttgg gctccttgct
    14341 cagcagcctg acgtactaaa cgctctgcgg tttggatatt tgttgccaca tccttagcgc
    14401 attgcatctg aatggttgca actcttacat ttctcatctt tttctcctat tctggaattt
    14461 gttgggtgat acagtggata ttgccaccac ctaagagaat atctctggct ggtattccga
    14521 caactttacg gtctgggaaa cacttgctga ggatatctaa ggccacttgg tcgtttacat
    14581 cctcaaactg tggaaccaag acagccttgt tggcgatata aaagtttacg taggaagctg
    14641 ctagtcgttc acctgcgtat cgctcttctt ctccttcttc atagatgtag cctggcaaat
    14701 cttcttctgt cacaacttgt cgaactgcag ggataggcaa tttatgaatg gtgaagtgac
    14761 aaccttttgc atctgtttcc tgttctaaga gttcgagatc tgcttttgac atggcatact
    14821 ggggatcatt ttcgtcatct gtccaagcca aaacaagctc agcaggacca acaaaggcag
    14881 caacattatc gacgtgttca ttggtttcat cctgataaat accataagga agccaaataa
    14941 ctttttcagc accaagactt tctaataatg tgttttcaat ctcctcttta gtcaggttag
    15001 gattgcgacc aggactaagc aagcaacttt cagttacgag aatagttcct tgaccatcgc
    15061 tatggattgc gcctccttcc agtacaaaag gtttagcatc atagacaggc ttttccaagg
    15121 cctcagcaaa acgactggct acttggtcat cctcttcata atcttgataa agaccatcat
    15181 aggtgcctcc ccaagcattg aaggcccaat ccacggctaa tttcttacct ttatcattga
    15241 cgagaatggt tgggccagta tcacgcgccc aggcatcatt ggtgggaatg tctaaataaa
    15301 caaccttgtc tccaagatag gattgggctt cagatagata ggcctgctcc accaaaagat
    15361 agactctttc cccttctgct atggtctcga tgatctgagt aaatgctctt ttagcagcct
    15421 ttccttgaaa aggccatgat cctggtcgag tcggccatat catgagggta ccatgatggg
    15481 gttcgtactc tgctggcatg tgatagccta atttttttgg actgtccatc atgataatct
    15541 ccctttaaag tcttgatagc caaaagcttt gagtaagcta cagtctccct gttcgtccat
    15601 gagatagaga cttggcaatc caataccatt aaaggtatta tttttgacaa aagaataaat
    15661 ggccatgtct tgaaaataaa gtctgtctcc gatttggact ggattttcaa aactataatc
    15721 accaatcaca tcgccagtca gacaggtatt agaagaaagt ctgtaggtat gggctttttc
    15781 ctgtgactca aagccatttc tcaaaggtgg acgatagggc atctcaagta catcaggcat
    15841 atggcaggtc gcagaggcgt ctaaaaccaa gatttccata ccgttttcta caatatctaa
    15901 tacctcagtt gctaaataac ccgcattaag cgcaatggct tcaccaggct cgatatagat
    15961 ttcaagattg taagtttttc ggatacgctt gatttctgaa atcagcaaat ccacatcgta
    16021 accttctctt gtaatatgat gaccaccacc catattgagc cattttacct catgtaagta
    16081 gggaccaaac tgttcttcta ctgctttcaa agttgtttgt aaatcatctg ctccctgctc
    16141 gcaaagggta tgaaaatgaa gtccgtcaac caaatctagc aaatcactcg gaatcttgtc
    16201 tatagtaact ccaaagcgag aaccaggtgc acaagggtca tagagcgcgt gatctccttg
    16261 agttgaacac tgagggttga ggcgcaaacc aacactgaca ccagcctctc gacaacgcga
    16321 accatgctta cgcaactgtc tctctgagtt aaagacgata tggtctgtta tcccaagcaa
    16381 ttcctccatg tcctcatcct tgaaagctgg tgcaaaaaca tggacttcac caggaaattc
    16441 ttcccttgcc aatttggcct catagagtcc actagctgtc gtacctgata gatactggct
    16501 aatcaaggga taagttttgt agagggaata tgccttctgg gcaagcaaga ccttgcaacc
    16561 ggcctcttct tgtacatatt gtagaatgcg gcaattagct tctaacttgg ccaagtcaat
    16621 aacataggct ggtgttggta cttgttctaa cttcattagt ccaccatttg tggattttca
    16681 accacaaccc atggcaaacc atactcattc aaagcttcca tgaatggatc tggatctaac
    16741 tcctcaaggt tatacactcc agcttgtttc caagtaccgt tcataactaa ttttgtccca
    16801 atcatggctg gaacgcctgt tgtgtaagaa atagcttgtg aaccaacttc tgcgtaacat
    16861 tcctgatggt cgcaaacatt gtagatatag atggtctttt caacaccgtc cttgacacct
    16921 gtaaagatac aaccgatatt ggttttacca acagtgcgcg ggccaaggct tgcagggtct
    16981 ggaagcaagg cttttaagaa ttgaatcgga acaatgtctt gaccgttgaa gttaatagca
    17041 tctgtacgaa ggagtccaac attttcaaga catttcatgt gcgtcaagta agattgacca
    17101 aaggtcataa agaaacgaat acgtttaaca cctggaatgt tcttagccaa tgattcgatt
    17161 tcttcgtggt gaaggagata catgtctttt tgtccaactt gagggaaatc gtactcgcgc
    17221 ttgatagaca tggcttcaac ctcgacccat tttccatctt cccagtaaga acctggcgca
    17281 gaaacctcgc gtagattgat ttctgggtta aagtttgtcg caaatggata accgtggtca
    17341 ccaccattac agtctaaaat gtcgatatag tggatttcat caaaatagtg tttgagggca
    17401 taagccgaga aaacactagt cacacctggg tcaaaaccag aaccaagcag agcggtcaag
    17461 ccagcttctt tgaatttctc ctgataagcc cattgccatg agtagtcaaa gtaggctgta
    17521 aaaccaagtt ccttgcaacg tttttcataa atagcacgcc actcagggtc ttctgtatcc
    17581 tcagcctcgt agttggctgt atcgatatag tgaacacctg ttgccaagca agcatccatg
    17641 atggtcaagt cttgataagg caaagctacg ttcaacacag cttctggttt gtagctttca
    17701 atcagggcaa tcacttcttc aaccttatca gcatcaagag ctgctgtctc aatccttgta
    17761 cttgttttgc cttccagttt agctttcaag tcatcacatt ttgacttagt acggctagca
    17821 atcataatct ctgtaaaggt ttcgctatct tgacaaatct ttgaaatagc aacttgggca
    17881 acgcccccac atccaataac tagtaaacga ctcatttttt tccttcctct tcttctaaaa
    17941 tgtcctcaac atacttgggc aacataaagg ctcccacgtg taagtttgca gtgtagtatt
    18001 ctgtgaaaag ctgacgtttt ttccagcctt ccttgtcaaa atctttgaca gggtggtatt
    18061 tttttgatgc aaatccaaac aaccaatagc cagctggact ggttgggata tgggcctgat
    18121 agacccgact gattggaaag gcttgattaa ccttgcgatg catgcttcgg caagctgact
    18181 catcctcatc aaagaaggga ctcccatgct gataaatcat gataccatct tcctttagag
    18241 ctcgataact attaccgtaa aattccttgg taaagagccc ttccgtatgg ccaaatggat
    18301 ctgtcgcatc gttgatgata atatcgtagt catcttcgca gtttcgcaaa aagcgtagcc
    18361 cattttgata ataaatggta acacgaggat catctagccc tgcggcaaaa tctgggaaat
    18421 actcacgaca gacttcaacc aacatctcat ctggttccac aatgtcgatt tgttccagct
    18481 caggatagag tgttaatact tgggcaacac cgccgtcacc acctccaata accaatactt
    18541 tctttggatt tgggtggaca gccatgggaa cgtggacggt catttcattg tagacaaaat
    18601 catccgcatc tgaaaacaaa acatgcccat ttaaaatcag tattttccca aaagctggcg
    18661 tatccaagac ttcgatatcc tgccattcac tttttccagc gtaaagttgc ttggctgttc
    18721 tcagagacaa tttgacatct ggagtatgaa cttcagaaaa ccataaatcc atctatttct
    18781 ccttatatta tatttagcgc agttagctac tgccaaagcc caagtggtat acttggaata
    18841 agccactgtg gattagttca tttcctccaa tcgcttgacg atttccgaaa ggctctaacc
    18901 acagc
//
LOCUS       DBERDK010000039        18900 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.039, whole genome shotgun sequence.
ACCESSION   DBERDK010000039 DBERDK010000000
VERSION     DBERDK010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 18900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18900
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.039"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            177..2105
                     /gene="glgB"
                     /locus_tag="ACL73L_001308"
     CDS             177..2105
                     /gene="glgB"
                     /locus_tag="ACL73L_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HGS0772280.1"
                     /translation="MDNREALKTFMTGENFYLQHYLGAHREELNGEHGYTFRVWAPNA
                     QAVHLVGDFTNWIENQIPMVRNDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPF
                     AVRYEARPGTGAILTELPEKKWKDGLWLARRKRWGFEERPVNIYEVHAGSWKRNSDGS
                     PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQ
                     DFVEECHTHNIGVIVDWVPGHFTINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDL
                     GKNEVQSFLISCIKHWIDVYHLDGIRVDAVSNMLYLDYDNAPWTPNKDGGNLNYEGYY
                     FLQRLNEVIKLEYPDVMMIAEESSSATKITGMKEIGGLGFDYKWNMGWMNDILRFYEE
                     DPIYRKYDFNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFASLRNL
                     YTYQICHPGKKLLFMGSEYGQFLEWKSEEQLEWSNLEDPMNAKMKYFTSQLNQFYKDH
                     RCLWEIDTSYDGIEIIDADNRDQSVLSFIRKGKKGEMLVCIFNMVPVERKDFTIGLPV
                     AGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTLPAMGASVWKIKRRL
                     KSTKTVTNKNQKGVENEK"
     gene            2095..3237
                     /locus_tag="ACL73L_001309"
     CDS             2095..3237
                     /locus_tag="ACL73L_001309"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HGS0772281.1"
                     /translation="MKNEMLALILAGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGIHNVGVVTQYQPLALNNHIGNGSSWGLDGINSGVSILQPYSASEGNRWFEGT
                     SHAIYQNIDYIDSVNPEYVLILSGDHIYKMDYDDMLQSHKDNNASLTVAVLDVPLKEA
                     SRFGIMNTDANNRIVEFEEKPAQPKSTKASMGIYIFDWQRLRNMLVAAEKSKVGMSDF
                     GKNVIPNYLESGESVYAYEFSGYWKDVGTIESLWEANMEYISPENALDSRNRQWKIYS
                     RNLISPPNFLGANAHVEDSLVVDGCFVDGTVKHSILSTGAQVREGAEVLDSVIMSGAI
                     IGQGAKIKRAIIGEGAIISDGVEIDGTDEVQVVGYNEVVGVATDED"
     gene            3227..4366
                     /gene="glgD"
                     /locus_tag="ACL73L_001310"
     CDS             3227..4366
                     /gene="glgD"
                     /locus_tag="ACL73L_001310"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HGS0772282.1"
                     /translation="MKIDKYSAILGNTVGFHNMSTLTDHRPVASLPFGGKYRLIDFPL
                     SSLANAGVRSVFGIFQQDNISSVFDHIRSGREWGLSTLLSHYYLGIYNTRVESSTVGK
                     EYYQQLLTYLKRSGSNQTVALNCDVLINIDLNQVFHLHSTTKEPITVVYKKLAKKDIS
                     EVNAILDVDETDHVLSHKLFDSKSTAETFNMSTDIFVVDTPWLIEHLEEEAKKEHPEK
                     FRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDMLESQKFYSLFSPNQKIYTK
                     VKNEEPTYYANTSKVSTSQFASGSIIEGQVANSVLSRNIHVHKDSLVKDSLLFPRVVI
                     GEGAQVEYAILDKGVEVEHGVVIRGTAEHPVVVKKGAKVTEDIHS"
     gene            4363..5796
                     /gene="glgA"
                     /locus_tag="ACL73L_001311"
     CDS             4363..5796
                     /gene="glgA"
                     /locus_tag="ACL73L_001311"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HGS0772283.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYY
                     DMVEAKFGNQIEDVLHFEVSVGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLAAIEAMERIDFIPDLLHVHDYHTAMIPFLLKEKYRWIQAYEDIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGFERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDQILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWFAQIYPDKLSTNITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            5855..6229
                     /locus_tag="ACL73L_001312"
     CDS             5855..6229
                     /locus_tag="ACL73L_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HGS0772284.1"
                     /translation="MSQVKGLCVLDVDGTLILEEVIDLLGREAGHEAEISQITSRAMR
                     GELVFESSLRKRVSLLEGLPILVFDNVFNSIHLSLNVPEFISILQKNGILVGLVCGVW
                     WIPIVGEISKIPWYCLFHCQPA"
     gene            6174..6503
                     /locus_tag="ACL73L_001313"
     CDS             6174..6503
                     /locus_tag="ACL73L_001313"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HGS0772285.1"
                     /translation="MERLAKSLGIAYFTANQLEVKEGLLTGKLVEQIISPQVKKETLE
                     KWRKKLKLSKERTVAIGDGVNNLLMLKSAELGIAFCAKEVLKKEIPHHVDKRDFLEVL
                     PLIDCLE"
     gene            6512..7611
                     /locus_tag="ACL73L_001314"
                     /pseudo
     CDS             6512..7611
                     /locus_tag="ACL73L_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
     gene            complement(7608..8054)
                     /locus_tag="ACL73L_001315"
     CDS             complement(7608..8054)
                     /locus_tag="ACL73L_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HGS0772286.1"
                     /translation="MTDLSKQLLEKAHGGLKINPDEQRRYLGTFEERVLGYVDIDTAN
                     SPQLEKGFLFILENLQEKAEPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHIT
                     SPFGLVIHSNAPVQVEEKDLRLAFPKLWEVKKEEPAKTSLWKKWFS"
     gene            8218..9522
                     /gene="eno"
                     /locus_tag="ACL73L_001316"
     CDS             8218..9522
                     /gene="eno"
                     /locus_tag="ACL73L_001316"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-displayed alpha-enolase"
                     /protein_id="HGS0772287.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
     gene            complement(9660..10043)
                     /locus_tag="ACL73L_001317"
     CDS             complement(9660..10043)
                     /locus_tag="ACL73L_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGS0772288.1"
                     /translation="MFVSYFNLLFSIDYINCLSETTDKNWHKSDRIFVTNTGKPVHSS
                     ILSKSLQRANERLKKPIPKHLSPHIFRHTTISILSENKIPLKTITDRVGHSDSEVTTS
                     IYTHVTKNMKDEAINVLDKVMKKIF"
     gene            10141..10296
                     /locus_tag="ACL73L_001318"
                     /pseudo
     CDS             10141..10296
                     /locus_tag="ACL73L_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
     gene            10496..13771
                     /gene="rexB"
                     /locus_tag="ACL73L_001319"
     CDS             10496..13771
                     /gene="rexB"
                     /locus_tag="ACL73L_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HGS0772289.1"
                     /translation="MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKER
                     AVLECLSQQASFSITVTRFAQMARYLVLNDLPVKTTLDDIGLGLAFYKCLAELDPKDL
                     RVYGAIKQDPQLIQQLIELYHEMTKSQMSFLDLENLTDEDKRTDLLLIFEKVTAYLNQ
                     GQLAQGSQLSHLIEAIENDKVSSDFNQITLVIDGFTRFSAEEERVVDLLHGKGVEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKVSRLLE
                     SSYDFSELALDVDEKDRENLQIWSCLTQKEELELVARSIRQKLHENSDLSYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEAMAHHPLTQFVESILALKRYRFRQEDLINLL
                     RTDLYTDLSQSDIDAFEQYIRYLGINGLPAFQQTFTKSHHGKFNLERLNVLRLRILAP
                     LETLFASRKQKAENLLQKWSVFLKEGAVTKQLQDLTTTLEAVEQERQTEVWKAFCHVL
                     EQFATVFAGSQVSLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLTADFVYA
                     IGLTQDNLPKISQNTSLLTDEERQNLNQATEEGVQLLIASSENLKKNRYTMLSLVNSA
                     RKQLFLSAPSLFNESESKESAYLQELIHFGFRRREKRMNHKRLSKEDMGSYHSLLSSL
                     VAYHQQGEMSDTEQDLTFVKVLSRVIGKKLDQQGLENPAIPTSPSSKTLAKDTLQALY
                     PAKQEFYLSTSGLTEFYRNEYSYFLRYVLGLQEELRLHPDARSHGNFLHRIFERALQL
                     PNEDSFDQRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPAIE
                     TIKEEANFGGKDQAFIQLDNGRSVFVRGKVDRIDRLKANGAIGVVDYKSSLTQFQFPH
                     FFNGLNSQLPTYLAALKREGEQNFFGAMYLEMAEPVQSLMAVKNLAGAVVEASKSMKY
                     QGLFLEKESSYLGEFYNKNKANQLTDEEFQLLLDYNAYLYKKAAEKILAGRFAINPYT
                     ENGRSIAPYVQQHQAITGFEANYHLGQARFLKKLDLADSKRLVGEKLKQAWLEKIREE
                     LNR"
     gene            13768..17418
                     /gene="addA"
                     /locus_tag="ACL73L_001320"
     CDS             13768..17418
                     /gene="addA"
                     /locus_tag="ACL73L_001320"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HGS0772290.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSGQNIL
                     VSASAGSGKTFVMAERILDQLARGVEISQLFISTFTVKAATELKERLEKKISKKIQET
                     DDVELKQHLSRQLADLPNAAIGTMDSFTQKFLGKHGYLLDIAPNFRILQNQSEQLLLE
                     NEVFHEVFEAHYQGKQKETFSHLLKNFAGRGKDERGLRQQVYKIYDFLQSTSNPQKWL
                     SESFLKGFEKADFTSEKEKLTEQIKQALWDLESFFRYHLDNDAKEFAKAAYLENVQLI
                     LDEIDSLNQESDSQAYQAVLARVVAISKEKNGRALTNASRKADLKPLADTYNEERKTQ
                     FAKLGQLSDQIAILDYQERYHGDTWKLAKTFQSFMSDFVEAYRQRKRQENAFEFADIS
                     HYTIEILENFPQVRESYQERFHEVMVDEYQDTNHIQERMLELLSNGHNRFMVGDIKQS
                     IYRFRQADPQIFNEKFQRYAQNPQEGKLILLKENFRSSSEVLSATNDVFERLMDQEVG
                     EINYDNMHQLVFANTKLTPNPDNKAEFLLYDKDDTGEEEESQTETKLTGEMRLVIKEI
                     LKLHQEKGVAFKEIALLTSSRSRNDQILLALSEYGIPVKTDGEKNNYLQSLEVQVMLD
                     TLRVIHNPLQDYALVALMKSPMFGFDEDELARLSLQKAEDKVHENLYEKLVNAQKMAS
                     SQKGLIHTALAEKLKQFMDILASWRLYAKTHSLYDLIWKIYNDRFYYDYVGALPNGPA
                     RQANLYALALRADQFEKSNFKGLSRFIRMIDQVLEAQHDLASVAVAPPKDAVELMTIH
                     KSKGLEFPYVFILNMDQDFNKQDSMSEVILSRQNGLGVKYIAKMETGAVEDHYPKTIK
                     LSIPSLTYRQNEEELQLASYSEQMRLLYVAMTRAEKKLYLVGKGSREKLEAKQYPAAK
                     NGKLNSNTRLQARNFQDWLWAISKVFTKDKLNFSYRFIGEDQLTREAIGELETKSPLQ
                     DSSQADNRQSDTIKEALEMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGV
                     EIAGQGQSVGKKISFDLPDFSTKEKVTGAEIGSATHELMQRIDLSQQLTLASLTETLK
                     QVQTSQAVRDKINLDKILAFFDTVLGQEILANTDHLYREQPFSMLKRDQKSQEDFVVR
                     GILDGYLLYENKIVLFDYKTDRYDEPSQLVDRYRGQLALYEEALSRAYSIENIEKYLI
                     LLGKDEVQVVKV"
     gene            17588..18766
                     /locus_tag="ACL73L_001321"
     CDS             17588..18766
                     /locus_tag="ACL73L_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772291.1"
                     /translation="MTLPVRKSLHNAVLQASEADTWEQATKEWNEVSLIFNGIGRSNC
                     VCGNVIKYAYELFNGVTGQRLFPIGSDCVRHFHRLSLDQQLEEEEKLLRKVENLTRKA
                     QKKEKIKVNKSDFDERLLKWLWEKGVFKPNRGNQFAPERDYQLFLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERAIKAKQYQKELE
                     KDQRIASQDPIERKQKQTWLLNSYFRELPEEKARFSRLLLEYRKSGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDHRSSAQGSWIEGILLNCLKPFLERLVI
                     "
BASE COUNT         5760 a         3616 c         4096 g         5428 t
ORIGIN      
        1 aaaaagaaag gcgttttcgc aattttttga aaaaatattg atttacaatt gaacgagtaa
       61 gtgaacttgt tgtagatttt caaaataatt aaagaagtat agtaatttac ttgaatcttc
      121 tctaaaaagg tagtataata aaaacataga aaacgcttac aggagaaggg gagtatatgg
      181 ataatagaga agcattaaaa acctttatga cgggtgaaaa tttttatctc caacattatc
      241 taggagcaca tagggaagaa ctaaatggag agcatggcta taccttccgt gtttgggcac
      301 ctaatgctca ggctgttcac ttggttggtg attttaccaa ctggattgaa aatcagattc
      361 caatggtaag aaatgatttt ggggtctggg aagtctttac caatatggct caagaagggc
      421 atatttacaa atatcatgtc acacgtcaaa atggtcatca actgatgaag attgaccctt
      481 ttgctgtcag gtatgaggct cgtccaggaa caggggcaat cttaacagag cttcctgaga
      541 agaaatggaa ggatggactt tggctggcac gaagaaaacg ttggggcttt gaagagcgtc
      601 ctgtcaatat ttatgaagtt cacgctggat catggaaaag aaattctgat ggcagtcctt
      661 atagttttgc ccagctcaag gatgaactca ttccttatct cgttgaaatg aactatactc
      721 atattgagtt tatgcccttg atgtcccatc ctttgggctt gagttggggg tatcagctta
      781 tgggttactt cgctttagag catgcttatg gcagaccaga ggagtttcaa gattttgtcg
      841 aggagtgtca tacccataat attggggtta ttgtggactg ggtaccaggt cactttacca
      901 tcaacgatga tgccttagcc tattatgatg ggacaccgac ttttgaatac caagaccata
      961 ataaggctca taaccatggt tggggtgccc ttaattttga ccttggaaaa aatgaagtcc
     1021 agtccttctt aatttcttgc attaagcatt ggattgatgt ctatcatttg gatggtattc
     1081 gtgtggatgc tgttagcaac atgctctatt tggactatga taatgctcca tggacaccta
     1141 ataaagatgg cggaaatctc aactatgaag gttattattt tcttcaacgc ttgaatgaag
     1201 ttattaagtt agaatatcca gatgtgatga tgattgcaga agaaagttcg tctgcgacca
     1261 agattacggg aatgaaagag attggtggtc taggatttga ctacaaatgg aacatgggct
     1321 ggatgaatga tatcctccgt ttctacgaag aagatccgat ttatcgtaaa tatgacttta
     1381 acctggtgac tttcagcttt atgtatgttt tcaaggagaa ttatctcttg ccattctcgc
     1441 acgatgaagt ggttcatggc aagaagagta tgatgcataa gatgtgggga gatcgttaca
     1501 atcaattcgc aagcttgcgc aatctctata cgtaccaaat ttgtcaccct ggtaagaaat
     1561 tgctcttcat gggtagcgaa tacggtcaat tcctagaatg gaaatctgaa gaacagttgg
     1621 aatggtctaa cctagaagac ccaatgaatg ctaagatgaa gtatttcact tctcagctaa
     1681 accagtttta caaagatcat cgctgtctgt gggaaatcga taccagctat gatggtattg
     1741 aaatcattga tgcggataat cgagaccaga gtgttctttc ctttattcgt aagggtaaaa
     1801 agggagaaat gttagtctgt atctttaaca tggtacctgt tgagcggaaa gattttacaa
     1861 tcggactacc cgttgcagga atttacgaag aagtatggaa tactgagttg gaagagtggg
     1921 gaggcgtttg gaaagaacat aatcaaactg ttcaaacgca agaaggccta tggaaagatt
     1981 atgagcagac cttaaccttt actctaccgg ctatgggagc aagtgtatgg aaaatcaaac
     2041 gtcgcttgaa atctactaaa accgtcacaa ataaaaacca aaaaggagta gaaaatgaaa
     2101 aatgaaatgt tagctttgat tcttgctggt gggcaaggaa ctcgtctcgg taaactcact
     2161 caaagcatcg caaaaccagc tgtgcaattt ggtgggcgct accgtatcat tgactttgcc
     2221 ctatcaaact gtgccaactc agggattcat aatgttgggg tcgttacaca gtatcaacca
     2281 cttgctctca acaaccatat tgggaatggt tcaagctggg gactagacgg tattaattca
     2341 ggcgtctcta ttcttcaacc ttattctgca agtgaaggaa atcgttggtt tgagggaact
     2401 agtcacgcta tttaccaaaa tatcgactat atcgacagtg tcaatcctga gtatgtcttg
     2461 attttgtctg gggaccacat ctacaaaatg gactatgatg atatgctcca gtctcataag
     2521 gataataatg ccagcttgac agtagcagtt ttagacgtcc ctcttaaaga agcaagccgt
     2581 tttggtatca tgaacacaga tgctaacaat cgtattgttg aatttgaaga aaaaccagct
     2641 caacctaaat ctacaaaagc ttctatggga atctacattt ttgattggca acgccttcgt
     2701 aatatgttag tcgctgctga aaagagcaag gttggcatgt cagactttgg taaaaatgtc
     2761 attccaaatt accttgagtc aggtgaaagt gtttatgctt acgaatttag tggttattgg
     2821 aaagatgttg gtactattga gtcactttgg gaagcgaaca tggagtatat ttctccagaa
     2881 aatgccttgg atagtcgtaa ccgtcaatgg aagatttact caagaaactt gatttcacca
     2941 ccaaacttcc tcggggcaaa tgctcatgtg gaagactcat tagttgtaga cggatgtttc
     3001 gttgatggaa ctgttaaaca ttctatcctt tcaacaggcg cgcaagttcg cgaaggagcg
     3061 gaagtccttg attcagttat catgagtgga gctatcattg gtcaaggagc taagattaaa
     3121 cgtgccatta ttggtgaagg tgcgattatt tctgacggtg tcgaaattga tggaacagat
     3181 gaagtacaag ttgtaggata taatgaagta gtgggggtag caacagatga agattgataa
     3241 atattctgcc attttaggaa atacagttgg ttttcacaat atgtcgacat tgacagacca
     3301 tcgtccagta gcaagtttgc catttggtgg gaaatatcgt ttgattgact tcccactttc
     3361 aagccttgct aatgcaggtg ttcgtagtgt ctttggtatt ttccagcagg ataatatcag
     3421 ctcagtattt gaccatattc gttcaggacg cgagtggggc ttatcaaccc ttcttagtca
     3481 ttactatcta ggaatttaca atacccgtgt agaaagtagt acagttggaa aagaatacta
     3541 ccaacagctt cttacttatt tgaaacgttc tggctcaaac caaacggttg ccctcaactg
     3601 cgatgttctt attaacattg atttgaacca agttttccac ctacatagta caacaaaaga
     3661 gcctatcact gtagtttata aaaaactagc taagaaagat atttcagaag taaatgcaat
     3721 cttggatgta gatgaaacag accatgttct ttctcataaa ctctttgata gcaagtcaac
     3781 agctgaaacg ttcaatatgt ctacagatat ctttgtcgtt gatacacctt ggttgattga
     3841 acacttggaa gaagaagcta aaaaagaaca tccagagaaa tttcgctatg ttttacggga
     3901 tttggctgta aaagagggag ctttcgccta cgagtacacg ggctatctag ccaatattca
     3961 ctctgttaaa tcttattatc aagcgaatat cgatatgctt gaatcacaaa aattctattc
     4021 tcttttctca ccaaaccaaa agatttatac aaaggtcaaa aacgaagagc caacttacta
     4081 tgctaataca tctaaggtaa gcacttctca gtttgcctct ggtagtatta ttgaaggtca
     4141 agtagctaat tctgttctat cacgtaatat tcatgtccat aaggatagct tggttaaaga
     4201 tagcctgctc ttccctcgtg ttgttattgg agaaggggct caggtcgaat atgctatctt
     4261 ggacaaaggg gttgaagttg agcatggtgt tgtgattcga ggaactgcag aacatccagt
     4321 tgtggttaag aaaggtgcta aagtaacaga ggatattcat tcatgaaaat tttatttgta
     4381 gcagcagagg gtgcaccctt ttcaaaaaca ggtggtttgg gagacgtcat tggcgctctt
     4441 ccaaaatcac tggtaaaagc tgggcacgaa gttgcagtga ttttacccta ctatgatatg
     4501 gtagaggcta aatttggaaa tcagattgaa gatgtacttc attttgaggt gagcgttggt
     4561 tggcgcagac agtattgtgg aattaagaaa acagtattaa atggtgtaac cttctacttt
     4621 attgacaatc aatattattt cttccgtggt catgtttacg gtgattttga tgacggagaa
     4681 cgctttgcct ttttccaact ggctgccatt gaggctatgg aaaggattga ctttattcct
     4741 gatcttctcc atgttcatga ctaccataca gctatgattc ctttcttgtt gaaggaaaaa
     4801 taccgttgga ttcaagccta tgaggacatt gaaacagttt taaccattca taatttagaa
     4861 ttccaaggac aattttcaga aggaatgttg ggtgatttgt ttggagttgg ctttgaacgt
     4921 tacgctgatg gcacccttcg atggaacaac tgtctgaact ggatgaaggc aggtattctc
     4981 tatgcgaacc gtgtttcaac cgtttcacct agctatgctc atgaaattat gactagtcag
     5041 tttggatgta atttggatca gattcttaaa atggagtctg gtaaagtatc tggtatcgtg
     5101 aatgggattg atgctgatct ttataatcct cagacggatg ctcttttaga ctatcatttc
     5161 aatcaggaag atttgtctgg gaaagccaaa aataaggcaa aattgcaaga aagagttggc
     5221 ttgcctgtta gagcagacgt tccactggtg ggaattgttt ctcgtttgac acgtcaaaaa
     5281 ggttttgatg tggtggtcga aagtcttcac catatcttgc aagaagatgt tcagattgtt
     5341 cttttgggaa ctggcgatcc agcctttgaa ggagctttct catggtttgc tcagatttac
     5401 ccagacaagc tatcaacaaa tatcactttt gatgtcaaac ttgctcagga aatctacgct
     5461 gcttgtgacc tcttcctcat gccaagtcgt tttgaaccgt gtggcttgtc tcaaatgatg
     5521 gctatgcgtt atggaacctt gccattggtc catgaagttg gaggcttgcg agatacagtt
     5581 cgcgctttca atccaatcga aggaagcggt actggcttta gctttgacaa tctatctcct
     5641 tattggttaa attggacttt ccaaacagca ttggacttgt atagaaacca tccagacatt
     5701 tggagaaacc tacaaaaaca agctatggag agtgacttct catgggatac agcctgcaag
     5761 tcataccttg acttgtacca tagtttagtt aattaataga taaaatcgta tgatgacttc
     5821 atacgatttt taggctgttt agagaggaga actgatgtct caagtaaaag gcttgtgtgt
     5881 tttggatgta gatggaacct taatcctaga agaagtgatt gatcttttag ggagagaggc
     5941 aggtcatgag gcggaaattt cgcagattac aagtcgggca atgcgaggag agttggtctt
     6001 tgaaagcagt ttaagaaaaa gagtgtcctt gttggaaggt cttcctattt tggtctttga
     6061 taacgtcttc aactcaattc atctatcgct aaatgtccca gagttcatct ctattctcca
     6121 aaagaatggc atcctagttg gtctggtgtg tggtgtctgg tggataccaa tagttggaga
     6181 gattagcaaa atcccttggt attgcctatt tcactgccaa ccagcttgaa gtcaaagaag
     6241 gtcttttaac aggaaaatta gttgaacaaa ttataagtcc ccaggtcaaa aaagagactc
     6301 tggaaaaatg gagaaagaaa ctaaaacttt ctaaagaaag aacggtggca atcggtgatg
     6361 gggtcaataa tctattaatg ttgaagtcgg cggagttagg aatcgccttt tgtgccaaag
     6421 aagtgctcaa aaaagaaata ccacatcatg ttgacaagag ggatttttta gaagttcttc
     6481 ctttgattga ctgtttagaa tgagggaaaa tatgaagatt gtaattgcac cagattcgtt
     6541 taaggaaagt ttgacagctc aacaggtagc tgaagcaata aaaagaggct tccaacaatc
     6601 gatagcagat gtagaatatc tcctctgccc tgttggtgat gggggagaag gtactgtaga
     6661 tgctatccga cattctcttg acctaaaatg acagaccctt ttggacaaaa agaagccatg
     6721 cgctattttc aaaaagggga actggcacta tttgaagtag ctgacttggt tggtcttgga
     6781 aaaattccgc tagagaaacg aaatccactt caaatccaaa cttatggtat tggagagttg
     6841 attctccatc tcatttctaa agggattaaa gatatttata tcggtgttgg tggcacggcc
     6901 agtaatgatg gaggactggg gattgctgct ggtttaggtt atcaatttta tgatagggat
     6961 ggaaatgtct tgcctgcttc cggtcaatcc ttattagact tagcttctgt ttcaacagaa
     7021 aattgctata aaattcctga aggtgttcaa attcatattc tagcagatgt cgtgagtccc
     7081 ttatgtggtc atcaaggtgc gacttacact tttggcaatc aaaaaggtct acatccgact
     7141 atgtttgcag tcgtagatca ggcgatccaa gatttttatg aaaaattctc acctgcaaca
     7201 ttagaaatta aaggagcagg agcaggtgga ggccttgctg gtggtttgtg tgcctttgct
     7261 caggcaagta tcgtgtctgg aattgatacc tgcttggact taatcaactt tgataagaaa
     7321 gtttcagatg ctgacttggt tgttgttgga gaaggaagac ttgatagtca aagctttgct
     7381 gggaaagcgc ctataggtgt tgcaaaaaga acccctgttg gagttcctgt tattgctatt
     7441 tgcggtagtc ttgctgaaga tttacctccc ctaccatttg aaaatatcca agctgtcttt
     7501 tctattttgg agaaaagtga acctttagaa gatagtttga aaaatgccag tctctatctg
     7561 gagcgcacgg ctgccaatat tgggcactta ttaaatatgt gcaagattta gctaaaccat
     7621 ttcttccata aggatgtttt ggctggttct tcctttttaa cttcccaaag ttttggaaaa
     7681 gcaagtcgaa ggtctttttc ttctacttga actggtgcat tgctatgaat aaccaggcca
     7741 aaaggagaag taatatgctc ttcagatact atggtggctt gactatcagt ttcttttgct
     7801 tcttttaagt agaaaacttg cttatcaaat tcgatagttg gtgaaatctt cacaaatagt
     7861 ggctctgctt tttcctgaag gttttctaaa ataaataaaa agcctttttc taactgaggg
     7921 ctatttgctg tgtcaatatc tacatatcca agaactcttt cctcaaaagt accaagatag
     7981 cgtctttgct catccggatt tatttttaac ccaccatgag ctttttcaag taattgtttt
     8041 gataaatctg tcatgaaaac agtatatcat aaaagttaga ataaaacaga caaaagaaag
     8101 cgattacttt ataaagttaa ggaaaagttg cacaaagata acgttttttc ttgaaaaagg
     8161 aggggtttta aggtattata gaggtgtaaa aaatttaact cataaggaga gtaaaaaatg
     8221 tcaattatta ctgatgttta cgctcgcgaa gtcctagact cacgcggtaa cccaacactt
     8281 gaagtagaag tttacactga atcaggtgct ttcggacgtg gtatggttcc atcaggagct
     8341 tctactggtg aacacgaagc agttgaactt cgcgacggtg acaaatctcg ttacggtggt
     8401 cttggtacac aaaaagctgt tgacaacgta aacaacatca ttgctgaagc tatcattggc
     8461 tacgatgtac gtgatcaaca agctattgac cgtgctatga tcgcacttga cggtactcct
     8521 aacaaaggta aattgggtgc gaatgcaatc ctcggtgtgt ctatcgctgt agctcgtgct
     8581 gctgctgact accttgaaat cccactttac agctaccttg gtggattcaa cactaaagtt
     8641 cttccaactc caatgatgaa catcatcaac ggtggttctc actctgacgc tccaatcgct
     8701 ttccaagagt tcatgatctt gccagttggt gcgccaacat ttaaagaagc ccttcgttac
     8761 ggtgctgaaa tcttccacgc tcttaagaaa atccttaaat cacgtggttt ggaaactgcc
     8821 gtaggtgacg aaggtggatt cgctcctcgt ttcgaaggaa ctgaagatgg tgttgaaact
     8881 atccttgctg cgattgaagc tgctggatat gtaccaggta aagacgtatt tattggattt
     8941 gactgtgctt catcagaatt ctacgataaa gaacgtaaag tttacgacta cactaaattt
     9001 gaaggtgaag gtgctgctgt tcgtacatct gcagaacaaa tcgactacct tgaagaattg
     9061 gttaacaaat acccaatcat cactattgaa gatggtatgg atgaaaacga ctgggatggt
     9121 tggaaagctc ttactgaacg tcttggtaag aaagtacaac ttgttggtga cgacttcttc
     9181 gtaacaaaca ctgactacct tgcacgtggt atccaagaag gtgctgctaa ctcaatcctt
     9241 atcaaagtta accaaatcgg tactcttact gaaacttttg aagctatcga aatggctaaa
     9301 gaagctggtt acactgctgt tgtatcacac cgttcaggtg aaactgaaga ttcaacaatc
     9361 gctgatatcg cagttgcaac taacgcagga caaatcaaga ctggttcact ttcacgtaca
     9421 gaccgcatcg ctaaatacaa ccaattgctt cgtatcgaag accaacttgg tgaagtagct
     9481 gaatatcgtg gattgaaatc attctacaac cttaaaaaat aaaatagttc agtgaactat
     9541 tttatcccga accatgaaat tcaaaagttc ggccgttgat ttaacaacgt ttctagcccc
     9601 tcggatttta tccgaagggc tatttttgta tttagaggac aaaaaagggg caaaactttt
     9661 taaaaaatct ttttcataac tttatccagt acattgattg cttcatcttt catgttcttt
     9721 gtgacgtggg tgtagatgga agtagtgact tcagagtcgg aatgaccaac cctgtccgtg
     9781 attgttttta aaggaatttt attttctgat aagatgctaa tagtggtgtg tctgaagatg
     9841 tgaggggaca gatgtttggg aattggtttt ttaaggcgtt cgtttgctcg ctggagagac
     9901 ttgctcaaga tagaactatg aactggtttt cctgtattgg taacaaaaat acggtcgctt
     9961 ttgtgccaat tcttatcggt tgtttcgctt agacaattta tatagtcaat tgaaaatagt
    10021 agattgaaat aagatacgaa cagcttgatt agggaaatca aattaatttc tagaaatatt
    10081 ttagcatcca cggtgtacta ttatagtttc aatttactat agttcaatca ttctatcaag
    10141 aatattgaat atataaaggc acgtaatatt aaaacttctt ctgatatcga attagagtcc
    10201 gatgttgatg gcaataaatc agacaatcta cccgtcgaca ttaagacact gggacaacac
    10261 attgaagtgt attccgtacc taagaaacaa tcataaaact caaagccttg ttaccgaggc
    10321 ttttttatta tatgattttc agtggccaga cttattctat ctaatacaag taaagctaag
    10381 tatttaagat ttagcagatg atgaagaaaa gaaaggatgg cctaaatatt ttaacagatt
    10441 tacgaggcta atcatggtat aatatagggt gatgaaaaat tccaacgagg ctgagatgaa
    10501 attactttat actgatattc gaacttcttt gacagaaatt ctaacaagag aggcagaaga
    10561 gctagttgca gctggcaagc gggtcttcta cattgccccc aactctcttt cttttgaaaa
    10621 ggaacgcgcc gtgctggaat gcttgtccca gcaggcttct ttttcgatta ccgtcacgcg
    10681 ctttgctcaa atggctcgtt atctggtctt gaatgattta ccagttaaaa ctactcttga
    10741 tgatatcggt cttgggttgg ccttttacaa atgccttgcc gaactcgatc ccaaggactt
    10801 gcgtgtttat ggcgctatta agcaggatcc tcaattgatc cagcagttaa ttgagcttta
    10861 ccatgagatg accaaatctc agatgagttt tttggacttg gagaatttaa cagatgagga
    10921 taagaggacg gatttactct tgatttttga gaaagtaaca gcctatctta atcaaggtca
    10981 gttagcccag ggaagtcagt tgtcccattt gattgaggct attgagaatg acaaggtaag
    11041 tagtgatttt aatcaaatca ccttggtcat tgacggcttt actcgttttt ctgctgagga
    11101 agagcgggtt gtggacttac ttcacggcaa aggtgttgag attgttatcg gggcttatgc
    11161 tagtaagaaa gcctatacca gtccttttag cgagggcaat ctctaccaag ccagcgtaaa
    11221 atttctccat catctggctt ctaaatacca aacgcctgct caggactgtt ctcaaactca
    11281 tgagaagatg gatagttttg acaaggtctc tcgtttgttg gagtcttctt atgacttttc
    11341 agaactcgct ttggatgtcg atgagaaaga ccgtgaaaat ttacaaatct ggtcttgttt
    11401 gacgcaaaag gaggagttgg agctagtagc ccgtagtatt cgtcagaaat tacatgagaa
    11461 ctcagacctg agctacaagc attttcgtat tctcttgggg gatgtagctt cttaccagtt
    11521 atctctcaaa actatttttg accagtatca gattcctttt tatcttggta gaagcgaagc
    11581 catggctcat catcccttga ctcagtttgt cgagtctatt ttagctttaa aacgttaccg
    11641 ttttcgtcag gaggatttga ttaatcttct tagaactgat ttgtatactg acctcagtca
    11701 gtctgatatt gatgcttttg agcaatatat ccgctatctt ggtatcaatg gcttgccagc
    11761 ctttcagcaa accttcacca aatcccacca tggaaaattt aatcttgagc gtttgaatgt
    11821 cctccgcctg agaattttag cacctcttga aaccctcttt gccagccgaa aacaaaaggc
    11881 tgaaaacctc ctacaaaaat ggagtgtctt tctaaaagaa ggagctgtga ctaagcagtt
    11941 acaagatttg acaaccactt tggaagctgt agaacaggaa agacaaaccg aagtttggaa
    12001 ggctttctgc catgttttag aacaatttgc gactgttttt gctggttcac aggttagtct
    12061 ggaagacttc ctagccttgc tccattctgg aatgagtttg tcccaatacc gtaccattcc
    12121 agcaacagtg gacactgttc tggtgcagag ttacgatttg attgcaccat tgactgctga
    12181 ctttgtctat gctattggac taactcagga caatttacca aaaatttctc aaaacaccag
    12241 tcttctgaca gatgaagaaa ggcaaaacct aaaccaagcg accgaagaag gcgttcaatt
    12301 actgattgcc agcagtgaaa atctcaagaa aaatcgctac actatgcttt ccttggtcaa
    12361 ttctgctcgt aagcagttgt tcttgtcggc tccaagcctt tttaacgaaa gtgaaagtaa
    12421 ggaatctgcc tatcttcaag agttgatcca ttttggattt aggcggagag agaagaggat
    12481 gaatcacaaa agactgtcta aggaggatat ggggtcctat cacagtcttt tgtctagtct
    12541 ggttgcctat caccagcagg gtgagatgag cgatactgag caagatttga cttttgtcaa
    12601 ggttctgtcg cgtgtcatag gtaaaaaact agatcagcaa ggtctggaaa atccagctat
    12661 cccaaccagt ccaagcagca agaccttagc caaggacacc ttgcaagctc tctatcctgc
    12721 caaacaggag ttttacctgt ctacgtcggg tttgacagag ttttatcgca atgaatacag
    12781 ttatttccta cgctacgttt taggcttgca ggaggaatta cgtttgcatc ctgatgcccg
    12841 tagtcacggg aatttcttgc atcgtatctt tgaacgcgcc ttacagttgc ctaatgaaga
    12901 ttcctttgac caacgtctag aacaagctat tcaagaaacc agtcaagaac gcgaatttga
    12961 agctatttat caagaaagtt tggaagccca gtttaccaag gaagttttgc ttgatgttgc
    13021 acggacgact ggacatattc tccgacacaa tccagccatc gaaaccatca aagaagaagc
    13081 aaattttggt ggaaaagacc aagcctttat tcaattagac aatggacgca gtgtctttgt
    13141 acgaggcaag gtggaccgga ttgaccgttt gaaagctaat ggagcgatag gagtagtaga
    13201 ctacaaatcc agtctgactc agttccagtt tcctcatttc tttaatgggc tcaattctca
    13261 gttaccaacc tatcttgctg ccctaaaaag agaaggggag cagaactttt tcggcgccat
    13321 gtacttggaa atggctgaac ctgtccaatc tctgatggcg gtaaaaaatc ttgcaggagc
    13381 agtggtagaa gccagcaaat ctatgaaata ccaagggctc ttcttggaaa aagaaagcag
    13441 ttatttaggc gaattttata acaaaaacaa ggctaatcaa ctgacagatg aggaatttca
    13501 gctcctactg gactacaatg cctatcttta caagaaagct gctgagaaga ttttagcagg
    13561 ccggttcgcc atcaatcctt atactgaaaa tggcagaagc attgccccat acgtccagca
    13621 acatcaggct attacaggct ttgaagccaa ttaccatctg ggccaagccc gtttcctaaa
    13681 aaagttggac ctagctgata gcaagcgtct ggtcggagaa aaactcaagc aagcttggct
    13741 tgaaaaaata agagaggagt tgaatcgatg aagcttattc cctttttaag tgaggaggag
    13801 attcaaaaac tgcaagaagc agaagcaaat tcgagcaagg aacagaagaa aactgccgag
    13861 caaatcgaag ctatctacac ttctggccag aatatcctgg tctcagcatc ggctggttct
    13921 ggaaagacct ttgtcatggc agagcgcatt ctggaccaat tggcgcgtgg tgtcgaaatt
    13981 tctcaactct ttatctcaac ctttaccgtc aaggctgcaa ctgaacttaa agaacgttta
    14041 gagaaaaaaa tcagcaagaa aatccaagaa acagatgatg tcgagctcaa acaacacttg
    14101 agtcgccagt tggcagacct acccaacgct gccatcggaa ccatggactc tttcacacaa
    14161 aaattccttg gcaaacatgg ttatctgctt gatattgcac ctaatttccg tattttacaa
    14221 aaccaaagcg agcaacttct tctcgaaaac gaagtctttc atgaggtctt tgaagcgcat
    14281 taccaaggta aacagaaaga gacctttagt catttgctga aaaactttgc tgggcgtggc
    14341 aaggacgaac ggggtctgcg ccagcaggtc tataaaatct atgacttcct ccaatccacc
    14401 agtaatcctc aaaagtggct gagtgaatct ttcctcaaag gatttgagaa agctgatttt
    14461 accagtgaaa aagaaaaact gactgagcaa atcaaacaag ccctttggga tttggaaagc
    14521 tttttccgtt accatctgga taacgatgcc aaggagtttg caaaggctgc ctatttagaa
    14581 aatgttcagt taattctgga tgaaattgac tccctaaatc aggagtccga tagtcaggct
    14641 tatcaggcag tgcttgcgcg tgttgtcgcc atctctaagg agaaaaacgg tcgagctctg
    14701 actaatgcca gccgtaaggc tgatttgaag cccctggctg atacctacaa cgaagagaga
    14761 aagacccagt ttgctaaact aggacaatta tcagaccaga tagcgattct cgactatcaa
    14821 gaacgttatc atggagacac ttggaaacta gctaaaacct tccaatcttt catgagcgat
    14881 tttgtagagg cttatcgtca gagaaaacga caggaaaatg cctttgaatt cgctgatatc
    14941 agccattaca ccattgagat tttagagaat ttcccacaag ttcgtgagtc ttatcaggag
    15001 cgcttccatg aagtcatggt cgatgagtat caggatacca accatattca agaacggatg
    15061 ctggaattgt tgtctaatgg ccacaatcgc tttatggtgg gagatatcaa gcaatccatc
    15121 tatcgtttcc gtcaggcaga cccgcagatt ttcaatgaga aattccaacg ctatgcgcaa
    15181 aatcctcaag aaggcaagct cattctcctc aaggaaaatt tccgtagtag ttcagaagtg
    15241 ctgtcagcaa ccaatgatgt ctttgaacgt ctcatggacc aagaggtcgg cgaaatcaac
    15301 tatgataaca tgcaccagct tgtttttgcc aataccaaac tgactcccaa tccagacaac
    15361 aaggccgaat ttctcctcta cgataaggac gatacaggtg aggaagaaga gagtcaaaca
    15421 gaaacgaaac taacaggcga aatgcgctta gttatcaagg agattctgaa acttcatcaa
    15481 gaaaaaggtg ttgcctttaa ggaaattgcc cttctgacct ccagccgcag tcgtaatgac
    15541 cagattctcc tcgccctgtc tgagtacgga attcctgtca aaactgacgg agagaaaaac
    15601 aattatctcc aatccctaga agtgcaagtc atgctagaca ctcttcgtgt cattcacaat
    15661 cccctgcaag actacgcctt ggttgccctt atgaagtctc caatgtttgg ttttgatgag
    15721 gatgagctag cacgtttgtc ccttcagaaa gcagaggata aagtccacga aaatctctat
    15781 gagaaactgg tcaatgcaca aaaaatggca agtagtcaaa aaggtttgat tcacacagct
    15841 ctagctgaaa aactaaagca attcatggat atcctagctt cttggcgctt gtatgccaaa
    15901 acccactctc tctatgactt gatttggaag atttacaacg accgttttta ttatgactat
    15961 gttggggctt tgccgaatgg tcctgctagg caggccaatc tctatgccct agcactgcgt
    16021 gctgatcaat ttgaaaagag caatttcaaa ggtttatcgc gttttattcg tatgattgac
    16081 caagtcttag aagcccagca cgatttggca agcgtggccg tcgcaccgcc aaaagatgca
    16141 gtagagctca tgaccatcca caagagtaag gggctggagt ttccttacgt ctttatcctc
    16201 aatatggatc aagatttcaa caagcaagac tctatgtcag aagtcattct cagtcgtcag
    16261 aatggtcttg gtgtcaaata tattgccaag atggagacag gggcagtaga agaccactat
    16321 cctaaaacca tcaaactctc cattcctagc ctgacctata ggcagaatga agaggaatta
    16381 cagctagcaa gctattctga gcagatgcgt ttgctgtatg ttgctatgac gcgggctgag
    16441 aaaaagctct atcttgtcgg caagggttct cgtgaaaagc tggaagccaa gcaataccca
    16501 gcagccaaaa atgggaaact aaatagcaat actagactgc aagcacggaa tttccaagat
    16561 tggctttggg ctatcagtaa agtgtttact aaggacaagc tcaactttag ttatcgtttt
    16621 attggcgaag atcagttgac cagagaagct atcggagagt tggaaaccaa gagtcctctc
    16681 caagatagct cccaagcaga caatcgtcag tcagatacca tcaaagaagc tctggaaatg
    16741 ctgaaggagg tggaagttta taatactctt caccgcgcag ctattgaact tcctagtgtt
    16801 caaaccccaa gtcaaatcaa gaaattctac gaaccagtta tggatatgga aggtgtcgag
    16861 attgctggtc aaggtcagtc agtaggcaag aaaatcagct tcgatttgcc agatttttca
    16921 acaaaagaaa aggtaactgg agctgagatt ggtagtgcta ctcacgaact catgcagaga
    16981 attgacctca gccagcaact aacccttgct agcctaacag aaacactcaa acaagttcaa
    17041 actagccaag ctgtcagaga caagatcaat cttgataaaa ttcttgcttt ctttgacaca
    17101 gtactcggtc aggaaattct tgctaatacc gaccatctct atcgcgagca acctttctcc
    17161 atgctcaaac gagaccaaaa gagtcaggaa gactttgttg tccgtggtat ccttgatggc
    17221 tatctgcttt acgaaaacaa aattgttctg ttcgactaca agacagaccg ctatgatgaa
    17281 ccaagtcaac tcgtagaccg ctatcgtggt cagttagctc tatacgaaga ggctttatca
    17341 cgagcctatt cgattgaaaa tattgaaaaa tacttgattt tactcggtaa agacgaggtt
    17401 caagttgtaa aagtataact tatactcttc gaaaatctct tcaaaccacg tcagcttcgc
    17461 cttgctgtat atatggttac tgacttcgtc agttctatcc acaacctcaa aacagtgttt
    17521 tgagctgact gcggctagct ttctagtttg ctctttgatt tttattgagt attagaaagg
    17581 agacctcatg acacttccag ttagaaaatc cctacacaat gcggttttac aggcttcaga
    17641 agcggatact tgggaacaag ctaccaagga atggaatgaa gtttccttga tttttaacgg
    17701 tatcggccgt agcaattgtg tctgtggaaa tgtcatcaaa tacgcttatg aactctttaa
    17761 cggtgtcaca ggccaacgcc tctttccaat tggtagcgac tgtgttcgtc attttcatcg
    17821 attgtccctt gaccagcaat tggaagagga agaaaaactg ctcagaaagg ttgaaaatct
    17881 aaccagaaaa gctcagaaaa aggaaaaaat caaggtcaat aaaagtgact ttgacgagcg
    17941 acttctaaaa tggctctggg aaaaaggtgt tttcaaaccc aatcgtggca atcaatttgc
    18001 gcctgagaga gactaccagc ttttcctaga agtctttcag ggaggaagtt ggaccaaggc
    18061 ggaaccaaag aaaaaggctc ggatggaaga agtccttgaa aagtgtatca aacccttttt
    18121 acttgggaaa tcggatgacc aactctacct tgtaaagcta ggcaaggaga aaattgacta
    18181 cgaacaagaa ctccgtatcc aggcagagaa agaacgcaag aagagggata aaatcgccaa
    18241 gcaatatgca gacaatctgg ttctagccat gggacccgca gagcgtgctt atcaagatta
    18301 ctttggcttt acagagaccc tgactcaaga agaacgcaag tgggaaaaaa ttctttttgg
    18361 taaaaataga gcagaacggg ctatcaaggc caaacaatac caaaaagagc tggaaaaaga
    18421 ccaacgaatt gccagtcagg atccaattga aagaaaacag aagcagactt ggctcctcaa
    18481 ttcctatttt cgtgagcttc ctgaagaaaa agctcgattt tctagactct tattagaata
    18541 tagaaaaagt ggtgaagtac ccttttcaac tgaatacctg tcagatcatc tcatcgactt
    18601 tttctataaa atgaaagcct ttgaatttga aatcgcacca gaacaagtcc gagattttct
    18661 aaaaaaaagc ctgcaggaag accatcgctc atcagcacaa ggaagctgga tagaaggaat
    18721 tctcctcaac tgcctcaaac catttttaga acgattagtg atatgagtaa cacctaccgt
    18781 gtaaatttca tggtaggtgt tattgtacct ccaaataata taaaacaaag tatgaaagcg
    18841 catctcaatt ttaagacttt tttgtaaatt aagagtataa ttaaggtata ctgccttttc
//
LOCUS       DBERDK010000040        16695 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.040, whole genome shotgun sequence.
ACCESSION   DBERDK010000040 DBERDK010000000
VERSION     DBERDK010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 16695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16695
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.040"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            59..637
                     /locus_tag="ACL73L_001322"
                     /pseudo
     CDS             59..637
                     /locus_tag="ACL73L_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558202.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(736..2358)
                     /gene="groL"
                     /locus_tag="ACL73L_001323"
     CDS             complement(736..2358)
                     /gene="groL"
                     /locus_tag="ACL73L_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HGS0772292.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISHRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALANVIPAVATLELTGDEVTGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAELGIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
     gene            complement(2374..2658)
                     /gene="groES"
                     /locus_tag="ACL73L_001324"
     CDS             complement(2374..2658)
                     /gene="groES"
                     /locus_tag="ACL73L_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HGS0772293.1"
                     /translation="MLKPLGDRVLLKIEEKEQTVGGFVLAGSAQEKTKTAQVVATGQG
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDEKYIIVGEANILAIIEE"
     gene            complement(2814..3209)
                     /locus_tag="ACL73L_001325"
     CDS             complement(2814..3209)
                     /locus_tag="ACL73L_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGS0772294.1"
                     /translation="MYNKVIMIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGER
                     EADFVNMVLWGRLAETLASYATKGSLISVDGELRTRRFEKNGQMNYVTEVLVTGFQLL
                     ESRAQRAMRENNAGQDLADLVLEEEELPF"
     gene            complement(3287..4048)
                     /locus_tag="ACL73L_001326"
     CDS             complement(3287..4048)
                     /locus_tag="ACL73L_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HGS0772295.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEALKA
                     EFAETFPNQTVWTFLLDVTDMTIVKTVCSDILETIGQIDILVNNAGLALGLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTRCFLPAMVKANQGHIINMGSTAGIYAYAGAAVYSATKAA
                     VKTFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIEALQAQDI
                     ADTVVYVTSQPRRVQITDMTIMANQQATGFMIHKK"
     gene            complement(4081..4707)
                     /gene="ytpR"
                     /locus_tag="ACL73L_001327"
     CDS             complement(4081..4707)
                     /gene="ytpR"
                     /locus_tag="ACL73L_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HGS0772296.1"
                     /translation="MIFTYNKEHVGDVLMVIVKNSGDAKLNMERKGKVARVFLKENGE
                     TVAWNIFEVSSLFEIAERGQVFLSDEQVARLNQELQAEGFTEEIVNDKEPKFVVGEIV
                     EMVAHPDSDHLNICQVAVASDKIVQIVAGAPNARVGLKTIVALPGAMMPKGNFIFPGE
                     LRGEKSFGMMCSPRELHLPNAPQKRGVLELSEDQVVGTPFDPAKHWTA"
     gene            complement(4723..5040)
                     /locus_tag="ACL73L_001328"
     CDS             complement(4723..5040)
                     /locus_tag="ACL73L_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HGS0772297.1"
                     /translation="MIQPASLEELASLVEKAGKKVFLFVADWCGDCRYIYPALPEIEE
                     TNPEFTFIRMDRDQYMDLAKLWDVYGIPSLVVLEKDKEIGRFVNRDRKSKEQINDFLA
                     GLK"
     gene            complement(5037..5336)
                     /locus_tag="ACL73L_001329"
     CDS             complement(5037..5336)
                     /locus_tag="ACL73L_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HGS0772298.1"
                     /translation="MNGMKAKKMWMAGLALLGIGSLALATKKVADDRKLMKTQEELTE
                     IVRDHFSDMGEIATFYVQVYESSLESLVGGVIFEDGRHYTFVYENEDLVYEEEVL"
     gene            complement(5534..5731)
                     /locus_tag="ACL73L_001330"
                     /pseudo
     CDS             complement(5534..5731)
                     /locus_tag="ACL73L_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724444.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
     gene            complement(5973..6647)
                     /locus_tag="ACL73L_001331"
     CDS             complement(5973..6647)
                     /locus_tag="ACL73L_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772299.1"
                     /translation="MKKKAFGIVLLVLAAWILLQGNFGIPSLDGKIWPLLGIVFFAYK
                     SIESILRRHLTSAVFTGLLALIIANYAYDLLPVTNHSLIWASILVVLGVGYLTHSSKF
                     WNEKKWWYNGKKTVVTDKEVAFGSGTFYKQDQDLVDDQVEVAFGDAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWRVDLKVETSFGAAKADAPVAPTSKTLIIRGDVAFGKL
                     EIVYVK"
     gene            complement(6653..7099)
                     /locus_tag="ACL73L_001332"
     CDS             complement(6653..7099)
                     /locus_tag="ACL73L_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HGS0772300.1"
                     /translation="MKLRIEIDGNLEETEIVIKTPTLTDEIADLQRLLQESKAPRLTF
                     YKGTGEYYLDLSEILFFETEGSKIYAHNQKEAYEVRLKLYELESILPRYFNRVSKSTI
                     ANIRQIYSVDKSFSGTGTISFYQTHKEVHVSRHYQSLLKENLRNMR"
     gene            complement(7213..7716)
                     /locus_tag="ACL73L_001333"
     CDS             complement(7213..7716)
                     /locus_tag="ACL73L_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HGS0772301.1"
                     /translation="MKNYQEWYRNISSRLTSRPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLGQQVGVYVLIPASGFVALSLFRKKINHPRPYETWDICPLLDKDSSGRSM
                     PSRHVFSATIISMACLHASLPIGLVCLIFSALLGLVRVLGGVHYPKDVLVGYACGLVW
                     GFLFFLF"
     gene            complement(7713..8075)
                     /locus_tag="ACL73L_001334"
     CDS             complement(7713..8075)
                     /locus_tag="ACL73L_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HGS0772302.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGGKAESVHQVTSWLTGYEVS
                     DVLACLDRDVTYGDFFRQAPYYVPERIAITGKICGVRIEEIDDPLMQEIRRLDKLVDW
                     LAKGKTSQQVLEKYEKHK"
     gene            complement(8141..9172)
                     /locus_tag="ACL73L_001335"
                     /pseudo
     CDS             complement(8141..9172)
                     /locus_tag="ACL73L_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155117.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(9179..10000)
                     /locus_tag="ACL73L_001336"
     CDS             complement(9179..10000)
                     /locus_tag="ACL73L_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS0772303.1"
                     /translation="MILLAILFTCFSVYLELEVPTYISKITDLLGSQGTNLDELWQPA
                     SMMMGMLFLAFLSVVAVGFFASRVAASYTSRLRSDIFNRVLDYSQTEIKKFSIPSLLT
                     RTTNDITQVQMLITMGLQVVTRGPIMAIWAIGKILGHSEYWLWAVLVAVIVNVLMTTV
                     LMTLDFPKQSLIQGLIDKLNSITRESLTGIRVVRAYNAEDYQNEKFAAVNDELTRLNL
                     FVNRLMAILNPIMMGISSGLSVAIYWIGAYVINDAAPIARLPLFSDMIVFMSYAM"
     gene            complement(10016..10487)
                     /locus_tag="ACL73L_001337"
                     /pseudo
     CDS             complement(10016..10487)
                     /locus_tag="ACL73L_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360543.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
     gene            complement(10611..11327)
                     /locus_tag="ACL73L_001338"
     CDS             complement(10611..11327)
                     /locus_tag="ACL73L_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGS0772304.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHIIDKAIDKAKGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEDLETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
     gene            complement(12208..13623)
                     /gene="ply"
                     /locus_tag="ACL73L_001339"
     CDS             complement(12208..13623)
                     /gene="ply"
                     /locus_tag="ACL73L_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HGS0772305.1"
                     /translation="MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQ
                     LKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTV
                     TVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQT
                     EWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTS
                     FLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWNELSYDHQGKEVL
                     TPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRK
                     RTISIWGTTLYPQVEDKVEND"
     gene            complement(13634..14044)
                     /locus_tag="ACL73L_001340"
     CDS             complement(13634..14044)
                     /locus_tag="ACL73L_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772306.1"
                     /translation="MIKRGDVVALYLPFPTISSDLAVKNHMYICIDNSMTKNKELVKN
                     QTFKPALLTRRLVKNFMIEEPDLARNPFTRPTLIDLDKVFMLDNTVIPTSYLARRRRN
                     VSEELYEEILDHLVQPRLISLNKSEFMQLNPGTY"
     gene            complement(14037..14645)
                     /locus_tag="ACL73L_001341"
     CDS             complement(14037..14645)
                     /locus_tag="ACL73L_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772307.1"
                     /translation="MSQSSYLSPLLWLKKEADKEKMSATQCQIFFFYYQMFELLFARE
                     SDMKDLCLGTKGFYFSQLEKNLLSGVSRFLKNLEGKVTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQTIGRLENPSLLSSQDRQHLMWIYQSALEKDYIVKVIGDKHFVL
                     KRQDATKLTARQTQTLEILSQSEDLVNPVYVTLGEKGVLLLD"
     gene            complement(14658..15116)
                     /locus_tag="ACL73L_001342"
     CDS             complement(14658..15116)
                     /locus_tag="ACL73L_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HGS0772308.1"
                     /translation="MTPEEMYLTERLDVQIAHFLKKSVQHRRRYKVLKITEIVAGFLI
                     AVFCAIPMPGDRYRLISVALSSLGLLCEGIINLYNAKENWISYQKTAQLLEKEKFLYQ
                     CQTEKYAGKTKAFALFVKTCEGLISEEINQWESIQSKEVAASADAPVKKE"
     gene            15241..15640
                     /locus_tag="ACL73L_001343"
                     /pseudo
     CDS             15241..15640
                     /locus_tag="ACL73L_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(16404..16664)
                     /locus_tag="ACL73L_001344"
     CDS             complement(16404..16664)
                     /locus_tag="ACL73L_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772309.1"
                     /translation="MRERSATGAQDLSKSIKKHLNDLTRLTASLLGDEKLSAITSSSA
                     VKADMHRFVIELEPVKSTILQNDDISLDQNEIFEILKNFLDG"
BASE COUNT         4918 a         3813 c         3000 g         4964 t
ORIGIN      
        1 gctttcaaat ttctaggctc aggctgaaac agtctcccag actgtttcac tcccgaatgc
       61 taaaatcgtt cttgatcgct ttcacattgt acaacatctt agccgtgcta tgagtcgtgt
      121 gcgtgttcaa attatgaacc aattggatcg aaaatcccat gaatacaagg ctatcaagcg
      181 ctactggaag ctcatacaac aggatagccg taaactgagt gataagcgat tttatcgtcc
      241 tacttttcgt atgcatttaa ccaataaaga gattttagac aagcttttga gctattcaca
      301 agacttgaaa catcactatc agctctatca actcttgctg tttcactttc agaataagga
      361 accggagaaa tttttcggac ttattgagga caatcttaag caagttcatc ctatttttca
      421 gactgtcttt aaaaccttcc tcaaagataa ggaaaagatt atcaacgccc ttcaactaca
      481 ctattctaac gccaaaccgg aagcgaccaa taatctcatc aaacttatca agcgcaatgc
      541 ctttggtttt cgaaactttg aaaacttcaa aaaacggatt tttatcgctt tgaacatcaa
      601 aaaagaaagg acgaaatttg tcctttctcg agcttagctg acttcaaccc actacagttg
      661 acaaagagcc tataaaaaga agggttagtc attccctcct tttgtgtttt ttataagttg
      721 ttttctatag aaagcttaca tcatcccgcc catcatgctt ggatccattg ctggagctgg
      781 ggctactggt tctggtttat tggctacgac tgcttctgtt gtcaaaatca agctggctac
      841 agatgctgca ttttgtaggg ctgaacgact cactttaact ggatcaatga taccttgatc
      901 aatcatgtta acccactcgc cagttgctgc gttgaatcct ataccaagct cagcattttt
      961 caaacgatcg ataacgatag atccttcaaa tcctgcattg tgagcaattt gacgaacagg
     1021 ttcttccaaa gcacggagaa caatattacg tcctgttact tcatctcctg tcaattccaa
     1081 ggtagcaaca gctggaatca cattggcaag agctgttcca ccacctgcaa caataccttc
     1141 ttcaacagct gcacgagtag cgttgagggc atcttcaatg cggagtttca tttctttcaa
     1201 ctcagtttca gttgcggctc cgaccttaat aaccgctaca ccacctgaca atttggccaa
     1261 gcgttcttgc aatttttcac ggtcaaattc agaagttgta gtttcgattt gagacttgat
     1321 aaccgcaaca cggtgagaaa tcgcttcagg atttcctgca ccttctacaa taaccgtgct
     1381 atctttgtcc acggtcactc tcgctgcttg accaagagct tcaattgtcg catctttcaa
     1441 ctcaagacca aggtcttctg tgataactgt tccgcctgtt aagatggcga tatcttcaag
     1501 catggctttg cgacggtcac caaaaccagg tgccttgact gctactacgt tgaaggttcc
     1561 acgaatcttg ttcaaaacaa gagttggaag agcctcacca tccacatcat ccgcaataat
     1621 caagagtgga cgattgcttt ggagaatgct ttccaaaagt ggcaagattt cttggatatt
     1681 ggaaattttc ttgtctgtaa tcaaaatgta cggattttca aggtcagcca ccattttttc
     1741 gctatctgtc accatgtact gtgaaaggta accacggtca aactgcattc cttctacgac
     1801 ttcaagctct gtttccatac cacgtgactc ttcgatggtg atgacaccgt ctttgccaac
     1861 tttttccatt gcttcagaga tgtactcacc aactttttca gaacgagaag atacggctgc
     1921 aacttgagcg atagcttctt tattggcaac agggatggcg ttgtttttca aagcttcaac
     1981 tgctgcggca actgctgttt caatcccacg acgaataccg attggatttg cacctgctgt
     2041 gacgtttttg attccttcac ggacgatagc ttgggtcaag actgttgcag tcgtagttcc
     2101 gtcacctgcg atatcattgg ttttagaagc tacttctgat actaacttag cacccatatt
     2161 ttcaaaatgg tcttccaatt cgatttcttt ggcaatggtc acaccgtcat tggtaatcaa
     2221 gggtgaaccg aatgactttt caagaacgac attgcgacct tttggtccca aggttacttt
     2281 aacagtgtct gcaaggatat cgacaccacg aaccatggct gaacgggcat ctgatgaaaa
     2341 tttaatttct tttgacatac ttactttctc cttctattcc tcaatgattg ccaaaatgtt
     2401 agcttcgcct acgatgatgt acttttcatc gccatctttg acatcaagac ctgcgtgggc
     2461 ttcaactaag acacgatctc cagttttaac acttggagca accaagtcac cgttcaaggt
     2521 acgaacacct tgtccagtag ccacaacttg agctgttttg gttttttctt gggctgagcc
     2581 tgcaaggaca aagcccccaa cggtttgttc tttttcttct atttttaaga gcacacggtc
     2641 ccctaatggt ttcaacatct gatttcctcc ataatgagat agatattatt agcactcttt
     2701 gaactggagt gctaattcat aattctattg tatcacttgg tcagaaatag tcaagaaaaa
     2761 agtctgactt tctcaagata aaaagcctga gaccaactca gactttttaa ttcttaaaat
     2821 ggcaattctt cctcttccaa gaccaaatct gccaaatctt ggcctgcatt attttcacgc
     2881 atagcacgtt gggcacgact ttccaagagt tggaatcctg tcacaaggac ttcggttaca
     2941 taattcatct gaccattttt ctcaaagcga cgggtacgca attctccatc aacggaaatg
     3001 agactacctt tggttgcgta gcttgccaaa gtttctgcta gtctgcccca tagaaccata
     3061 ttaacaaaat cagcttcacg ttcaccgttt tggtctttgt aacgacggtt gacagcgata
     3121 gttgctcgcg ctaccgactt gtcattgttg gttttgtgca attctggtgt agacgttaaa
     3181 cgcccaatca tgataacttt attatacata ttttcttcct cctacttatc tattcgtagg
     3241 aaatcaaaaa aagttacaga aatttgtaac ttttcgagga aatttcttat tttttatgaa
     3301 tcatgaaacc tgtcgcctgt tgattggcca taatggtcat atctgtaatc tgaacacggc
     3361 gaggctgact ggtcacatag actactgtgt ctgcaatatc ctgagcttgc aaggcttcta
     3421 ttccttggta aacggacgca gctcgttctt tatcaccatg aaaacgcact gtagaaaaat
     3481 ctgtttcgac aattccaggc tgaatggtcg tcaccttgat atccgttgcg atggtatcaa
     3541 ttcgcagtcc atctgaaaag gtcttaactg ccgccttggt ggctgagtaa acagctgcac
     3601 cagcataggc ataaattcct gcggttgacc ccatattgat aatatgacct tgattggctt
     3661 ttaccattgc tggcaagaaa cagcgagtga ctgccatcaa acctttgaca ttggtatcca
     3721 acatggtcag catatccaac tcttcatagt cttgataggg agctaagcca agagccagtc
     3781 ctgcgttatt gaccaggatg tcaatctgac ctatcgtttc taaaatatca gagcagacag
     3841 tctttactat tgtcatatcc gtgacatcta ggagaaaagt ccaaactgtt tgatttggaa
     3901 aagtttctgc aaactccgcc ttaagagctt ctagtctgtc tatccgtcgt cctgttagaa
     3961 cgacatcctc accctgctcc agataagcac gcgcaatcgc ttcaccgatt cctgaggttg
     4021 ctcccgtaat cactacattt tttgccatct tatttccttc tatcagatgc tgacaagttc
     4081 ctaggcagtc cagtgtttcg ctgggtcgaa cggtgttccg acaacttggt cttctgataa
     4141 ttcaagcacc ccacgttttt gcggagcatt tggcaaatgc aattcacgag gactacacat
     4201 catcccaaaa ctcttttcac cacgaagttc gcctgggaaa atgaaattcc cttttggcat
     4261 catggcacca ggaagagcca caatggtttt caacccaaca cgcgcattag gtgctcctgc
     4321 aacgatttgc actatcttgt cacttgcgac tgcaacttgg cagatgttga ggtggtcact
     4381 gtctggatga gctaccatct cgacaatttc accaacaaca aacttaggtt ccttatcatt
     4441 aacaatttct tctgtaaaac cttccgcctg taattcttgg ttcaaacgag cgacttgctc
     4501 atctgataaa aagacttgac cgcgctctgc aatttcaaac aagctcgaaa cctcaaaaat
     4561 gttccaagct actgtttccc cattttcttt gaggaaaaca cgggctacct tgcctttgcg
     4621 ttccatattc agtttggcat ctccgctatt tttcacgatg accataagga catcaccgac
     4681 atgttctttg ttatacgtaa aaatcattgt ttcctttttc tcctatttca atcctgctaa
     4741 aaaatcgtta atttgctcct tacttttacg gtcgcgattg acaaaacgac cgatttcctt
     4801 gtccttttct agaacaacaa ggctaggaat tccgtaaaca tcccagagtt tggccaaatc
     4861 catatactga tctcggtcca ttcgaataaa ggtgaactct ggattggtct cctcaatctc
     4921 tggtaaggca ggataaatat aacgacaatc gccacaccag tctgccacaa aaaggaagac
     4981 cttcttgccc gctttttcca ctaaagatgc taattcttct aaacttgctg gctgtatcat
     5041 aagacttcct cctcatagac taggtcttca ttttcataga caaaggtata atgacggcca
     5101 tcctcaaaaa tgacgccacc aaccaagctc tccagactgc tttcgtaaac ttgaacatag
     5161 aaggtcgcaa tttcccccat gtcggaaaaa tggtctcgca caatctctgt caactcttcc
     5221 tgagtcttca tgagcttacg gtcatctgca acttttttcg tggcaagagc aaggcttccg
     5281 atacctagca gagccaagcc tgccatccac atttttttag ctttcatacc attcatttta
     5341 acacaaaaaa ggcttcagga caaatgagga agcagcagaa aagcaagtaa aaagcctctt
     5401 cctttaagga aaaggacttc ttatactaag agcaaactag gaagctagcc gcaggctgct
     5461 caaaacacgg ttttgaggtt gtggataagg cgacgctgac gtggtttgaa gagattttca
     5521 aagagtatta ttcttattgc caggcgcctg agttgccaac gtagtaacca tcaggtgtgt
     5581 agatattgcg aagcatctta cctgatgaag ctagataata ccacttgcca ttgtctttga
     5641 cccaatcatt cgctagcatg gctccagaag aacttacata ataccattct cccttgtcat
     5701 aaacccaagt gcttgctttc atggctcctg agcaattaaa ggttcaaaaa actgtccaat
     5761 atcattcatt tttttaaagc attttacact acatcatcca cgctgtagga catagataca
     5821 tcaaatgtaa gagccaaaat atcgaaaaat atacaacttt taaaggcaac atttcatttt
     5881 ttaaagtcta acaaccatag aactgaaatc agagcaaggc atcaacttcc tactcttagt
     5941 acgtctattt tgatggttga agtgaagatt ttttatttaa cgtagacaat ttccaacttc
     6001 ccaaaagcca catctccacg gataatcaag gttttgctgg ttggggctac aggagcgtca
     6061 gccttagctg caccaaagga ggtttctact ttcaaatcta cacgccagtg ttgtggaaca
     6121 tagacggttg catttccgaa ggccacttca atatttaaag ttgcaaaatc acctagcatc
     6181 tctgcattat catagtagat tttagcatcc ccaaaagcga cttccacttg gtcatctacg
     6241 agatcttgat cttgcttata gaaggtcccg ctaccaaaag cgacttcctt atccgtgacg
     6301 actgtttttt tcccattgta ccaccatttt ttttcattcc agaacttact tgaatgcgtc
     6361 agataaccaa caccaagtac caccaagatg ctagcccaaa taagagaatg attggtaact
     6421 ggtaacaagt cataagcgta atttgcaatg atgagcgcca gtaaacctgt aaaaactgcc
     6481 gaagtgagat gacgtctaag gatggactca atggacttat aagcaaaaaa aacaatacct
     6541 agtaaaggcc atattttacc atccaaagaa ggaattccaa aattcccttg cagcaagatc
     6601 caagctgcta aaaccaataa aacaatacca aatgctttct ttttcatgtt ttttacctca
     6661 tgtttcttag attttctttt aggagggatt ggtaatgccg tgagacatga acctccttgt
     6721 gcgtctgata aaaggaaatg gtgcccgttc ctgaaaagga cttgtccact gagtaaatct
     6781 gacggatgtt tgcgatcgtt gacttggaaa ctcgattaaa atagcgaggc aagatagact
     6841 ccaactcata gagcttgagg cgaacctcat aagcttcctt ctggttatga gcgtagatct
     6901 tgctcccttc tgtttcaaag aagagaattt ctgacaggtc tagataatat tcacctgtcc
     6961 ccttgtaaaa agtcaacctc ggagcctttg actcttgcaa gagccgttgc aagtctgcaa
     7021 tttcatctgt caaagtgggg gtcttgatga caatttcagt ctcctctaaa ttgccgtcaa
     7081 tctcaattcg taacttcatc acatctcctt tctgactcta ctatatcaaa gctaagttag
     7141 gaatacaagg gcaaaaaagc aagtggtcaa tttgaagcag taagtggttg taggactagc
     7201 ttaacttaca tgtcagaata ggaagaaaag gaatccccac accaaaccac aagcatagcc
     7261 aaccaagaca tccttgggat aatgaacacc ccctaaaacc ctcaccaacc ccagcaaagc
     7321 tgaaaagatc aagcatacta agccaatagg tagactagca tgcagacagg ccatggagat
     7381 gatagttgcc gaaaagacat ggcgactggg catcgaccgt cccgaactat ccttatcaag
     7441 aaggggacag atatcccaag tttcataagg tctcgggtga ttgattttct tacgaaacag
     7501 ggacaaggcc acaaaacctg atgcaggaat aagcacataa accccaactt gctgaccaag
     7561 tcctagttgc aggtaagtgg tgactagcaa gatcagatag accaggggca tggcgactgt
     7621 catcaaacga ttgaaactgc gtaataggaa taaaagaatt ggacgactag ttagtctgga
     7681 gctgatattt cgataccatt cttgataatt tttcatttat gcttctcata cttttctaga
     7741 acttgttgag atgttttccc cttggcaagc caatccacta gcttgtccaa gcgacgtatt
     7801 tcctgcatca gaggatcatc aatttcctca atacggacac cgcaaatctt ccctgtaatt
     7861 gcaattcgtt cagggacata atatggtgct tgtctaaaaa aatctccgta agtaacatcc
     7921 ctatccagac aagcaagaac atcactcact tcataccctg tcaaccaact ggtcacttgg
     7981 tgaactgact ctgcctttcc accctttcgc tcaacttttg caatcaaggc taagtagaca
     8041 gcagcaaatt tcatattgta taatttctga ctcatacttc tctctttcta tgtgtactag
     8101 tataaataag aaaaagaagg ccgtcaagcc ttcttttgat ttattcttct gcttcatctt
     8161 ctgtaaattg actattgtac aagtcagcgt agaagccacc ttgcgccatc agttcctcat
     8221 agttgccttg ctcgatgata tttccatctt tcatgaccaa gatcaagtct gcatttcgga
     8281 tggttgacaa gcggtgggca atgacaaagg atgtgcgtca ttccatcaaa cggtccatgg
     8341 ctttttggat caattcctct gtccgtgtgt caacagaaga agtcgcctca tccaaaatca
     8401 aaagcggtgc atccttaaga agggcacgag caatagtcaa tagttgtttt tgtcctacag
     8461 acaaggtcac ggtgtcatcc aagatggtat catagccatc tggcaaggtc ataataaagt
     8521 ggtgaattcc cacagcctta ctagcttcca tcattcgttc atcactaatc cctatttgat
     8581 tatagatgag attgtctcga atagttcctt caaagagcca ggtatcctgc aagaccattg
     8641 aaaaggcatc atgcacttct gaacgcgtca tagccttggt atccacacca tcaatgcgaa
     8701 tacttccctt atcaatctca tagaatttca tcaaaagatt gacaatggtt gtcttaccag
     8761 ccccagtcgg cccaacaatg gcaacctttt gaccagcatg agctgtcgca gagaagtcat
     8821 agtcttgaac attgacaccg tccaccagaa tttctcctgc tgacacgtcg tagaaacgtg
     8881 gaatcagatt gaccagagtt gatttaccag aacctgttga cccaataaag gccactgttt
     8941 gaccagtttc tgctttaaag ctaacatgtt caataactgc ctccgaattt gccgcatagc
     9001 ggaaggtcac atccttaaac tcgacctgac ctttgaagtt ttcatcagtc agctgcactt
     9061 gaacagggtt ttggatagaa gaatgcaaat ctaaaacttg attaatccgc ttagcagaga
     9121 ccatagttcg gggaagaacg atgaagagtg ctcccatgag aaggaagccc atgacaacct
     9181 acatggcata agacatgaaa acaatcatgt cactaaagag aggcagacgc gctatcggag
     9241 cagcgtcgtt aatcacatag gccccaatcc agtaaatcgc tacactcaaa ccacttgaaa
     9301 tccccatcat gataggattc aaaatagcca taagacggtt gacaaacaaa ttcaaacggg
     9361 tcaattcatc atttactgct gcaaattttt cattttgata atcctctgca ttgtaggcac
     9421 gaacgacacg aatacctgtt aaactctcac gagtgatact gttcagttta tctatcagcc
     9481 cctgaatcaa ggactgtttt ggaaagtcta gcgtcatcaa aacggtcgtc atcaggacgt
     9541 tgacaatcac tgccacaagt acggcccaaa gccagtattc tgaatgacct aaaatcttcc
     9601 caatagccca aatagccata attggaccac gcgttaccac ttgcaagccc atagtaatca
     9661 acatttgaac ttgagtaatg tcattggtag tacgcgtcaa gaggctagga attgaaaatt
     9721 tcttaatctc tgtctgcgag taatccaaaa ctcggttaaa aatatcactt ctcagcctac
     9781 tagtataaga agccgccact cgggatgcaa aaaatccaac tgcaactacg gacaagaagg
     9841 caagaaagag cattcccatc atcatgcttg ccggctgcca caactcatct aaattagttc
     9901 cttgactacc tagcaaatcc gtaattttcg agatataggt cggcacttcc aactctagat
     9961 agaccgaaaa gcaagtaaag agaatggcta gtaaaatcat ccccattctt ttctactaat
    10021 tcttttggct aatttcttta ttctctcctc ctattccctt gatattttgc ctgtagttga
    10081 ccgagaactt tctcaaaaat cagtaattca tcttcatcaa tgtcttccat caactgcttg
    10141 tctatgcgtt caaaaaaagc cttaacctgt tgcatctgag aacgtgcttt gtccgtcaga
    10201 cgaacaaact tagcccgctt atcaacagga ctcgcctcca attccaccaa accattttgc
    10261 actatacgct taaccagatt actagcaaca gacttggtaa tattgagttc ctgctcgata
    10321 tctttaatca agaccaagtc ttggtttttc tcgcgattat ccaaaaaacg cacaacctga
    10381 ccttgcggcc cacccataaa ttcaatgccg caacgtttgg cttccttttg caccatcagg
    10441 taaatttgat gaccaaaacg cttaaagact aacatcggtt tatccataat ctcccccttc
    10501 taaataaaaa tagttctctg gagaataatt aaatttctat gagaactatt ttcttgatta
    10561 aaaaaatccc aagtgatttt ctcacttagg atcatgttct ataggttaaa ttaaaaccca
    10621 tctacgttcg tataaatctt ttggacgtct tcgtcgtctt caagaacgct gtaaagtttt
    10681 tcaaaggttt caaggtcttc gcctgacaat tccacttctg actgaggaat catttccaat
    10741 tcagtcactt ggaattcttc aataccagac tcacggagag caacgatagc cttgtgaagg
    10801 tcagttggcg ctgtgtaaac tgtgattgta ccttcttgtg cttctacgtc atccacatcc
    10861 acatccgctt cgagcaattg ctcaaagact gcgtccgcat cttcacctcc aaatacaata
    10921 acacctttgt tgtcaaaaag gtaagaaaca gaacctgaag cgcccatgtt tccgccgttt
    10981 ttaccaaagg ctgcacggac attggctgct gtacggttaa cgttagaagt caaagtatcc
    11041 acaattagca tagagccatt tggcccaaaa ccttcgtaac gtccttctgt aaaggtttcg
    11101 tctgtgtttc ctttggcttt atcaatcgct ttatcgataa tgtgttttgg cacttgggct
    11161 tgtttagcac ggtcgataac gaatttcaaa gctgagtttg attctggatc tggatcacct
    11221 tttttagctg ctacatagat ttctacacca aattttgcat atactttaga gttagctcca
    11281 tctttagccg ttttcttggc tacgatattg gcccatttac gtcccattag gaatctcctt
    11341 ttttcacatt ttaatctttc ttattataac acaagttttt ttgattttca ctagaggaaa
    11401 tggattttat tagcaaatca agctaggata gtactttacc tgctaagatg gtcttgcctt
    11461 tctatcttta tcaacagaca ctcatccaca ttcaaaaaac aaactagacc attatctgca
    11521 aatagaaagt ttcagccaag tttgacaaag tcagctcaaa ttactgtttg aagtttgtag
    11581 atataagcga caaaaacaat catactgcac cttttgttga cagtctactc cagacatatc
    11641 atagttccag taaatacttt gaaatccaac agttcttata ggcgctattg tattctaaga
    11701 aatcaataga agagtttcta agcaaacctc taatactcaa taaaaatcaa agagcaaact
    11761 agaaagctag cctcaggttg ctcaaaacac tgttttgagg ttgcggatag ggctgacatg
    11821 gtttgaagag attttcaaag agtataattt acgtgttccc aagatggaga agttagacta
    11881 gtacactggc acttctaaac cattgctagc aattgatttg ttcatattta atttcatttt
    11941 ttccataaat gggtattaga tataaacagc aaaatatttc cgatacgtgt cgttcttgaa
    12001 tttccaatca tctaaaacaa gtaaaggata atcaatcccc tgtatatcaa ggaattggct
    12061 acccttttta cttttttaca cattctgttt gatagattca ttttaacatc acgagcatac
    12121 tccaatggaa atcgctaggc aagagataaa ctttcagata tccgcagaga gatcatcgcc
    12181 tctttttgtc gcaagcattc tcctctccta gtcattttct accttatctt ctacctgagg
    12241 atagagagtt gttccccaaa tagaaatcgt ccgcttacgc actagtggca aatcggtttt
    12301 ttcataaacc gtacgccacc attcccaggc aagcccggta cactctctaa ttttgacaga
    12361 gagattacga acattccctt ttaaaggaat actagtggta aagtgagccg ttaaatcctg
    12421 cccatttctg tcccaagcct taggagtcaa gacttcctta ccttgatgat cataggataa
    12481 ttcattccaa gtaatataat attgggcaac ataggcacca ctatgatcca gcagtaaatc
    12541 tccgtttctg taagctgtaa ccttagtctc aacatagtct gtactatttt gaaaggtcgc
    12601 aactacattg tcacgtaaaa aagaagttgt ataggaaatc ggcaagcctg gatgatctgc
    12661 tgtaaagcga ctgccttctt gaatcaagtc ctctaccata tccaccttgc ctgttacaac
    12721 tcgggcaccc gaacttgggt cgccccctaa aataaccgcc ttcacttctg tattgtccaa
    12781 aatctgcttc cactctgtct gaggagctac cttgactcct tttatcaaag cttcaaaagc
    12841 agcctctact tcatcactct tactcgtggt ttccaacttg agatagactt ggcgcccata
    12901 agcaacactc gaaatataga ccaaaggacg ctctgcagaa attcctctct gttttaaatc
    12961 ctctaccgtt acagtatctt gaaacacatc tcctggattt ttaacagcgt ctacgctgac
    13021 tgtataataa atctgcttaa aattaacaat ctgaatctgc ttttcgcctg aatggacaga
    13081 gttaaaatca atatcaagag aattccctgt cttttcaaag tcagaaccaa acttgacctt
    13141 gagttgttcc atgctgtgag ccgtgatttt ttcatactgc attctagctg ggacattatt
    13201 gacctgacca taatcttgat gccacttagc caacaaatcg tttaccgctc cgcgaacact
    13261 tgaattgctg gggtcttcca cttggagaaa gctatcgcta cttgccaaac caggcaaatc
    13321 aatactataa gtcatcggag cacgatcgac cgcaagaaga gtgggattat tctctaacaa
    13381 ggtctcatcc actacgagaa gtgctccagg atagaggcga ctgtcgttgg tagctgttac
    13441 agaaatatca cttgtatttg tcgacaagct ccgcttcttt ctttcgataa caacaaactc
    13501 atcgggtagc tgattaccct ctttgatgaa acgattttca atactttctc cctgatgggt
    13561 caagagtttc tttttatcgt aattcatagc tagtataaag tcatttactg ctttatttgc
    13621 catcttctac ctcctaataa gttcctggat tgagttgcat aaactcagac ttgttcagcg
    13681 aaatcagccg tggttggact aagtgatcca aaatttcctc gtacaattct tctgagacat
    13741 tgcgtcgccg tctggctaga taagaagtcg gaataaccgt attatccaac ataaatacct
    13801 tatctaagtc aatcaaggtt ggtcttgtaa aaggattacg agctagatcc ggctcttcta
    13861 tcataaagtt cttgaccaaa cgtctggtca agagagctgg tttgaaggtc tgatttttaa
    13921 ccaactcttt gtttttagtc atgctgttgt caatacagat atacatatga ttcttcacag
    13981 ccaaatcgct actaatagtc ggaaaaggca aataaagagc tacaacatct cctctcttaa
    14041 tcaagcaaga gcaccccctt ttctcctaat gtaacataga caggattgac caagtcttct
    14101 gattgactca gaatttccaa agtttgagtt tggcgcgctg tcaatttagt agcatcttgt
    14161 ctcttcaata caaaatgctt gtcgccaata accttgacaa tataatcctt ctccaaagct
    14221 gactggtaaa tccacatcag atgttgtctg tcctgagaac tcaagagaga aggattttca
    14281 agcctcccga tagtctgata aaaatcaaaa acaggagcta actcctgcca atctgattgg
    14341 ctagttgtca aggctagaaa aagggctttg cgagctgata cttcttggtt agccttgaga
    14401 gttactttcc cctccaagtt ttttagaaat cgggaaactc cagaaagcaa atttttctct
    14461 aactgcgaga aataaaaacc tttcgttccc agacataagt ctttcatgtc gctttctcta
    14521 gcaaataaga gctcaaacat ttgatagtaa aagaaaaata tctggcactg ggtcgcgctc
    14581 atcttttcct tatcggcttc tttttttaac cagagcaagg gcgacaggta gctggattga
    14641 gacatttcct ctacctccta ctctttttta actggagcat ctgcactagc tgccacttct
    14701 tttgactgga tactttccca ctggttaatc tcctctgaga taagaccttc gcatgtcttg
    14761 acaaataggg caaaagcctt ggtctttcct gcatatttct ccgtttggca ttgatagagg
    14821 aatttttctt tctccaggag ttgcgcagtt ttttggtaag aaatccaatt ttcctttgca
    14881 ttatacaaat tgataatccc ctcacacagc aagccgagac tggataaggc aaccgaaatc
    14941 aaacggtagc gatcacctgg cataggaata gcacaaaaga cagctatgag gaaacctgcc
    15001 acgatttctg ttatttttaa taccttatag cgcctacgat gttgaacgct tttctttaaa
    15061 aaatgagcta tctgtacgtc taatcgctct gtcaggtaca tttcttctgg cgtcatattc
    15121 gtaactcctt tcatttactt tgataaccat agtataacat atttgaatat caaagtatag
    15181 taaaaactgg actataactt attagacttc ctgcgaaaca aaatatggta tagtagttct
    15241 atgaattatg aagcaagtaa acaactaact gatacacgat ttaagcgtct tgttggtgtt
    15301 cagcgtacca cttttgaaga gatgttagct gtattaaaaa cagcttatca aaaaagtcgg
    15361 acaagctggt aaaatcttgg ctgacagtaa ttatcaaggg ctcatgaaga tatatcctca
    15421 agcacaaact ccacgtaaat ccagcaaact caagccgcta atagctgaag ataaagccta
    15481 taaccgtgcg ctatctaagg agagaagcaa gattgagaac atctttgcca aagtaaaaac
    15541 gtttaaaata ttttcaacaa cctatcgaaa tcatcgtaaa cgcttcggat tacgaatgaa
    15601 tttgagtgct ggtattatca atcatgaact aggattctag ttttgcagga agtctattat
    15661 atatatgcac agacatatga aatctttttg atacagcctt ctttttacat agagagacag
    15721 cagttatgat atatttatcc atacacaaca tcttctgtac caatactaca atattggtac
    15781 attttgatta cacaatcgtt tcaaaaaata tgatggaagc ctgagtattt tatagtaatt
    15841 cccttcctat caactagatc aaccagttga gtgttgaatg ttgaaacacc ctagcctcag
    15901 caaaggacta tgatcagcat ttcctagcca ataggacgct ccaaatcacc tattctaagt
    15961 ccataactag cttggtcagt aagatacaga ctgcaaggcg agactgacgt ggttgatgag
    16021 atttttgaag agtattacaa tcatagattc accagtagac ttgaacgcag atttaactat
    16081 agtagattga aactagaata gtacacctct gcttctaaaa cattgttaga aatcgatttg
    16141 actgtcctga tcaatttgtc ctgttctcgt ttcattttac tatactaaaa aaccacctga
    16201 aacaaatcaa gtgactagag gataaaaaca aagactgggc aaaaagtttt gaaaagcaaa
    16261 aaacgcataa tatcaggtct tgaaaaacct tgatactata cgtttgattg tgggaagatt
    16321 gacttcattt tctcctgaaa ttgaagtttt ttctagtttc cagatataga cgaattcaat
    16381 atagccatct cgctacaatt attttaacca tcgagaaaat ttttcagaat ttcaaaaatt
    16441 tcattttgat ccaatgaaat gtcatcattt tgaagaatag ttgacttcac aggctctaat
    16501 tctatcacaa agcggtgcat gtctgctttt accgcactac ttgatgttat agccgataac
    16561 ttttcatctc ctagcaagga agctgtcaaa cgggtaaggt cattcaaatg ctttttaatg
    16621 gacttactta aatcttgagc acctgtagca gagcgctctc tcatatccag ccaagcttta
    16681 gccttaaaaa taatt
//
LOCUS       DBERDK010000041        16460 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.041, whole genome shotgun sequence.
ACCESSION   DBERDK010000041 DBERDK010000000
VERSION     DBERDK010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 16460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16460
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.041"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(126..842)
                     /locus_tag="ACL73L_001345"
     CDS             complement(126..842)
                     /locus_tag="ACL73L_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGS0772310.1"
                     /translation="MAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSIT
                     VVRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNK
                     LVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRF
                     KLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYS
                     ETYKRSDYYKIKFISCDRDH"
     gene            1190..2977
                     /locus_tag="ACL73L_001346"
     CDS             1190..2977
                     /locus_tag="ACL73L_001346"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HGS0772311.1"
                     /translation="MTRFEIRDDFYLDGKSFKILSGAIHYFRVPPEDWYHSLYNLKAL
                     GFNTVETYVAWNLHEPREGEFHFEGDLDLEKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPAWLLTKNMRIRSSDPAYIEAVGRYYDQLLPRLVPRLLDNGGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEECGVTCPLFTSDGPWRATLKAGTLIEEDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRDPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFETLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDAEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKWLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHSYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKGENL"
     gene            2974..3450
                     /locus_tag="ACL73L_001347"
     CDS             2974..3450
                     /locus_tag="ACL73L_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGS0772312.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPVEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVPLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            3478..4383
                     /locus_tag="ACL73L_001348"
     CDS             3478..4383
                     /locus_tag="ACL73L_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HGS0772313.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERFDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQSVVDFVEAYKWVADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVAGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVAVAAPSTPSESGEIEDDEF"
     gene            4370..5185
                     /locus_tag="ACL73L_001349"
     CDS             4370..5185
                     /locus_tag="ACL73L_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HGS0772314.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATVINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALALSALGHFALGM"
     gene            5192..5596
                     /locus_tag="ACL73L_001350"
     CDS             5192..5596
                     /locus_tag="ACL73L_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGS0772315.1"
                     /translation="MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEE
                     FTAKFEAVIEGLDDFLVFADLLGGTPCNVVSRLIMEGRDIDLYAGMNLPMVIEFINAS
                     LTGADADYKSRAAESIVKVNDLLAGFDDDEDE"
     gene            5895..7061
                     /locus_tag="ACL73L_001351"
     CDS             5895..7061
                     /locus_tag="ACL73L_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HGS0772316.1"
                     /translation="MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAKREEIAA
                     FLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVAKPAT
                     YLKKDVATVLVSFARSGNSPESVATVDLAKSLVDELYQVTITCAADGKLALQAHGDDR
                     NLLLLQPAVSNDAGFAMTSSFTSMMLTALLVFDPTEFAVKSERFEVVSSLARKVLDKV
                     EDVKELVDLDFNRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INDNTVVLVFGTTTAYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVALGCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEYQK"
     gene            7177..8214
                     /locus_tag="ACL73L_001352"
     CDS             7177..8214
                     /locus_tag="ACL73L_001352"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HGS0772317.1"
                     /translation="MKAYTERVFGNVEGEDVLAYRFETDGGYQLEVMTYGATILRYVA
                     PDKAGNFANVILGFDDFDSYVGNSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNAS
                     NCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYEI
                     IYKVTTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSIAPDGVPAKTPEAN
                     RDVVKHIYNGTLLKDIFAEEDEQIQLASGLDHPFALPAGHDNAGFLYDQNSGRFLLFK
                     TEAPCFVVYTANFVDESVIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTF
                     TSKTRYELVVK"
     gene            8422..9276
                     /locus_tag="ACL73L_001353"
     CDS             8422..9276
                     /locus_tag="ACL73L_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGS0772318.1"
                     /translation="MTYEYKSHIYLAETVLNVKDLASQTAFYQQVIGLEILSQTETES
                     ILGLGGKVLVQLIQAQESGEVREHYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA
                     DHGYSEAIYLEDLEGNGIELYRDKPVSTWNIREDGRIIGVTEALAAQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSSQFYQKVLGLENKFSVPGASWIAAGDYHHHLAVNEW
                     GGKGLASRKQGLPGLAYYVIEVAHKKELLTIAQRAQEVDAPIKWMTSSQLEITDSDGI
                     VTRIRLAR"
     gene            9426..9683
                     /locus_tag="ACL73L_001354"
     CDS             9426..9683
                     /locus_tag="ACL73L_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HGS0772319.1"
                     /translation="MTNSKYITCLKRSEGQLCGIQKMIEGDCDCADIVTQLTAVRSSV
                     ERVIEMIITENLTECINQPLDDSEAQKERLEKAIRYLIKRK"
     gene            complement(9725..10087)
                     /locus_tag="ACL73L_001355"
     CDS             complement(9725..10087)
                     /locus_tag="ACL73L_001355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772320.1"
                     /translation="MKIKVQTRKLAAGCSKHCFKVVDGTDEVSSKHCFEVVDRTDEVS
                     NHIYGKVKLTWFEEIFEEYKSLHNKTHITKVLMNCTPKVRQKKSNFLGSVHSTPDTMR
                     FFNFKDFLQLLLTHIKLF"
     gene            complement(10141..10437)
                     /locus_tag="ACL73L_001356"
     CDS             complement(10141..10437)
                     /locus_tag="ACL73L_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HGS0772321.1"
                     /translation="METSISMADFYGKYQNENLELIDVREAHEFQAGHAPGAKNLPLS
                     TLEQGYKELKPDHEYYVICQGGVRSASTCQFLSSQGLTVTNVEGGMNAWPGQVE"
     gene            complement(10427..10609)
                     /locus_tag="ACL73L_001357"
                     /pseudo
     CDS             complement(10427..10609)
                     /locus_tag="ACL73L_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691454.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
     gene            10658..11422
                     /locus_tag="ACL73L_001358"
     CDS             10658..11422
                     /locus_tag="ACL73L_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HGS0772322.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLGEEI
                     DRYAREVGANCVGEFVSATKTYPVYVINYKGEEVCLAQAPVGSAPAAQFMDWLIGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPCRYMEMQPEAIAAIEEVLE
                     DRGIPYEEVMTWTTDGFYRETAEKVAYRKEEGCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL"
     gene            complement(11662..11814)
                     /locus_tag="ACL73L_001359"
     CDS             complement(11662..11814)
                     /locus_tag="ACL73L_001359"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772323.1"
                     /translation="MRTKASLRKLVTGCSKHCFEVVDRTDEVSSKHCFEVADRTDEVS
                     NIYTAR"
     gene            12032..13411
                     /locus_tag="ACL73L_001360"
     CDS             12032..13411
                     /locus_tag="ACL73L_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HGS0772324.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLMTFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVYSIFLTTFLVESLGAILLSFRLIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVF
                     ARSELLGLPHANVARRTIAPRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLV
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
     gene            13425..14090
                     /locus_tag="ACL73L_001361"
     CDS             13425..14090
                     /locus_tag="ACL73L_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HGS0772325.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQDMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQSWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
     gene            14400..14939
                     /locus_tag="ACL73L_001362"
     CDS             14400..14939
                     /locus_tag="ACL73L_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGS0772326.1"
                     /translation="MKLLSIAISSYNAAAYLHYCVESLVIGGEQVGILIINDGSQDQT
                     QEIAECLASKYPNIVRAIYQENKCHGGAVNRGLAEASGRYFKVVDSDDWVDPRAYLKI
                     LETLQELESKGQEVDVFVTNFVYEKEGQSRKKSMSYDSVLPVRQIFGWDQVGNFSKGQ
                     YTMMHSLIYRTDLLRASQF"
     gene            15006..15416
                     /locus_tag="ACL73L_001363"
     CDS             15006..15416
                     /locus_tag="ACL73L_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HGS0772327.1"
                     /translation="MYYLPVDFYRYLIGREDQSVNEQVMIKCIDQQLKVNRLLIDQLD
                     LSQVSHPKMREYLLNHIELTTVISSTLLNRSGTAEHLAKKRQLWTYIQQKNPEVFQAI
                     RKTMLSCLTKHSVLPDRKLSNVVYQITKSVYGFN"
     gene            15532..>16460
                     /gene="pavB"
                     /locus_tag="ACL73L_001364"
     CDS             15532..>16460
                     /gene="pavB"
                     /locus_tag="ACL73L_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HGS0772328.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADV
                     VNPTPGQVLPEETSGTKEGDLSEKPGDTVLTQAKLEGVTGNTNSLPTPTERTEVSEET
                     NSSSLDTLFEKDEEAQKNPELTDVLKETVDTADVDGTQASPAETTPEQVKGGVKENTK
                     DSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAK
                     NPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRANGTQTYKATVKVYGNKDGK
                     ADLTNLVATKNVDININGLVAKETVEKAVKDNV"
BASE COUNT         4616 a         3082 c         3718 g         5044 t
ORIGIN      
        1 ttggttctat ttctataaaa tattccacaa attatagaat cttccaaaaa taggtaagcg
       61 ctaccttttt ggtgtggtat aataagcata gaaaaagccc gagcgattag gctcaggctt
      121 tcttcttagt gatcacggtc acatgagatg aatttaatct tgtagtaatc agatcgtttg
      181 taagtttcac tgtattctaa aacttggcca gttgattcga gtttggtgat tttagtttgt
      241 aggacagtag ggaattgttc atcgactccg aggactgaag ccgcatgttc tggagttgga
      301 aagactattt cgttgatttc ttcaaagtgt tcatcattca tgtgaatgtg gtagtctaac
      361 ttgaaacgat tatagataga actatagtat tcaaggtttg gataatttgc gttgatatat
      421 tgttctggga tgtaggatgt atggtagata taaacgacac cgtttgattc gcggatacgt
      481 tcaatcttgt agtagaattg atcgccgcgt agacccaatt tttccaagta aacaagcttg
      541 tttccgcgtt caattgaaag aacagttacc ttatcatctt tagcattgaa gagttcaata
      601 tctgaaaact ctacaagctt gtgtttgcgt gcacgtgaaa cgaaggttcc ttttccttgt
      661 tggcggacaa tatagccatc tttggcaagg tcgtttaagg cgcgaacaac tgtgatagag
      721 ctgacatcgt acattgaaat gagttctgct tcagtgtaaa atttatctcc actgctaaac
      781 tgcccagaga tgattttatt ttttaattcg tcttttatgt attgatattt gggaattgcc
      841 atatttttac ctctattttc cttctccata gaaaatttta ccataaaagc gaaaaaaata
      901 gtagtctttt gaataaaaaa ttagaataat agtttgaaaa attaagcgat atagggattg
      961 ctcatatttt atcaatgtat tctatacaaa attattttag atcctatatt ccgaatttaa
     1021 agagcgtttg aaatgggtct aaaaatgttt gaaactaatt gaatgtgtgt ataatagtaa
     1081 aaatgcagta aatatcccga caatttctat tgacaattca aacagattgg tttatgatta
     1141 atataacaac aaatgaaatc gcaaactttc gcggtcggaa ggtagtttta tgacacgatt
     1201 tgagatacga gatgatttct atctcgatgg aaaatcattt aagattttat ctggtgccat
     1261 tcattatttt agggttcctc cagaggattg gtatcattcg ctctataact tgaaggctct
     1321 tggttttaat acggtagaga cttatgttgc ttggaattta cacgagcctc gtgaaggtga
     1381 gtttcatttt gaaggtgatc tggatttaga gaaatttctc caaatagcgc aggatttggg
     1441 tctctacgca attgtgcgtc cgtctccatt tatctgtgcg gaatgggaat tcggtggctt
     1501 accagcttgg ctcttgacca agaacatgcg aattcgctca tccgacccag catatatcga
     1561 ggcagttggt cgctactatg atcagttatt gccaagactg gtgcctcgtt tgttggacaa
     1621 tggtggcaat attctcatga tgcaggttga aaatgagtat ggttcttacg gagaagataa
     1681 ggcttacctg agagcgattc gacagctaat ggaagagtgt ggcgtaacct gtcccctctt
     1741 tacatcagat ggtccatggc gagctactct gaaagctgga accttaattg aagaggacct
     1801 ctttgtaaca ggaaactttg gttctaaggc accttacaac ttttcgcaga tgcaggaatt
     1861 ctttgatgaa catggtaaga aatggccact catgtgtatg gagttctggg atggttggtt
     1921 caatcgctgg aaagaaccga ttatcacacg ggatcctaag gaattggcag atgcagttcg
     1981 agaggttttg gaacaaggct ctatcaatct ttacatgttc cacggtggta caaactttgg
     2041 tttcatgaat ggttgctcag ctcgaggaac tttggacctg ccacaagtta cgtcttatga
     2101 ttacgatgcc cttctggatg aagaaggaaa tccaactgct aaatatcttg cagtcaagaa
     2161 gatgatggca acacattttt cagagtatcc gcagttggaa ccactctaca aagagagtat
     2221 ggagttggat gctattccac tagttgaaaa agtttctttg tttgaaacct tagatagctt
     2281 gtcaagtcct gtagaaagtc tctatcctca aaagatggag gagctgggac aaagttatgg
     2341 ctacctactt tatcgaacag aaacaaactg ggatgcagaa gaagaaagac ttcgtatcat
     2401 tgatggtcga gatagggccc agctgtatgt cgatggtcag tgggttaaaa ctcaatatca
     2461 gacagagatt ggggaagata ttttttatca aggtaaaaag aaatggctat ctaggttaga
     2521 tatcttgata gaaaatatgg ggcgtgtcaa ctatgggcat aagttcttag cggatacgca
     2581 acgtaaggga attcggacag gggtctgtaa ggatctgcat ttcttactaa actggaaaca
     2641 ctatccactc ccactagaca atcctgagaa aattgatttt tcaaaaggat ggactcaagg
     2701 acaaccagcc ttttacgctt atgactttac agtcgaagag ccaaaagata cttacctaga
     2761 cttgtctgag tttggtaagg gggttgcctt tgtcaatggg cagaatctag gacgtttttg
     2821 gaacgttggc ccaactctct cactttatat ccctcatagc tatctcaagg aaggtgccaa
     2881 ccgcatcatt atctttgaaa cagaaggtca atataaagaa gagattcatt taactcgtaa
     2941 acctacacta aaacatataa agggggaaaa cttatgacaa ttgtaggatg ccgtattgat
     3001 ggacgtttga tccacggaca agtagccaat ctttgggctg gaaaactaaa tgtttcacgc
     3061 attatggttg tagacgacga agttgtcaac aacgatattg aaaagagtgg tttgaaactt
     3121 gcgacaccac caggtgtgaa attgagtatt ttgccagttg agaaagctgc agccaatatt
     3181 cttgctggca aatacgatag ccaacgtctc tttatcgtgg ctcgtaaacc agaccgcttc
     3241 cttggtttgg tagaagcagg tgtaccactt gaaaccctta atgttgggaa tatgtctcaa
     3301 acaccagaaa ctcgttctat tacacgttct atcaacgtag tagacaagga tgtggaagac
     3361 ttccacaaac tggcagaaaa aggtgttaaa cttactgctc agatggttcc aaatgatcca
     3421 atttcagact ttttgagctt attaaaatag gaaaaaattt tttaggaggt cattgttatg
     3481 atacaatggt ggcaaatttt acttctcact ttgtactcag cttatcaaat ctgtgatgag
     3541 ttgacgatcg tttcatctgc aggttcccct gtatttgctg gtttcattac tggtttaatc
     3601 atgggagatg tgactactgg tttacttatc ggtggtaact tgcaactgtt cgttcttggg
     3661 gttggtacct tcggtggtgc ttctcgtatc gacgcaactt ctggtgcggt tcttgcgaca
     3721 gccttctctg tttcacaagg aattgatgca ccgcttgcaa ttactacaat cgctgtacca
     3781 gtagcagctc tcttgactta cttcgacgtt cttggtcgta tgactactac cttcttcgct
     3841 caccgtgtgg atgctgcaat cgaacgcttt gactataaag gtattgaacg caactacttg
     3901 cttggtgcga ttccgtgggc tctatctcgt gcccttccag tcttctttgc ccttgctttt
     3961 ggtggtgcct ttgtacaatc agtagtagac ttcgttgaag cctacaaatg ggttgcagat
     4021 ggcttgacac ttgcaggacg tatgcttcca ggtcttggat ttgcaatctt gcttcgttac
     4081 cttccagtta aacgtaacct tcactacctt gctatgggat ttggtttgac agctatgttg
     4141 actgttcttt actcatatgt aacaggtctt ggtggcgctg ttgctggtat cgtaggtact
     4201 cttcctgctg aagttgctga aaaaattggt ttcgtgaaca acttcaaagg tttgtctatg
     4261 attggtattt ctatcgtagg tattttcctt gcagtgcttc acttcaaaaa tagccaaaaa
     4321 gtagctgtag cagcaccttc tacaccatca gaaagtgggg aaatcgaaga tgacgaattc
     4381 taattacaaa cttacaaaag aagattttaa tcaaatcaac aaacgtagct tgtttacttt
     4441 ccaattaggt tggaactacg aacgtatgca agcttctggt tacctttaca tgatcttgcc
     4501 tcagttgcgt aaaatgtatg gtgatggaac tcctgaattg aaagaaatga tgaaagttca
     4561 tactcaattc ttcaatactt caccattctt ccataccatt atcgctggtt ttgaccttgc
     4621 catggaagaa aaagatggtg taggttcaaa agacgctgtt aacggtatca agacaggttt
     4681 gatgggacca ttcgctcctc ttggggatac aatctttggt tcacttgtac ctgctatcat
     4741 ggggtcagtc gcagcaacta tggctatcgc tggccaacct tgggggatct tcctttggat
     4801 tgcagttgca gtagcgtatg acatcttccg ttggaaacag ttggaatttg cttacaaaga
     4861 aggggttaac cttatcaaca acatgcaaag taccttgaca gctttgattg acgctgcatc
     4921 tgtacttggt gtcttcatga tgggtgctct tgtagcaaca gtgattaact ttgaaatttc
     4981 ttacaagttg ccaatcggtg aaaagatgat tgatttccaa gacatcttga accaaatctt
     5041 cccacgtttg cttccagcaa tctttactgc ctttatcttc tggttgcttg gtaaaaaagg
     5101 tatgaactct actaaagcta tcggtattat tatcgtactt gctttggctc tttctgccct
     5161 tggtcacttt gcacttggaa tgtaattcct tatgactaaa tcattaattt tggtgagcca
     5221 tggtcgcttc tgtgaggagc ttagaggtag cacagaaatg attatgggcc cacaagacaa
     5281 catttacaca gtagctcttc ttccagaaga tggcccagaa gaatttactg ctaaatttga
     5341 agctgttatt gaaggattgg atgatttcct agtctttgcg gatcttctcg gtgggacacc
     5401 ttgtaatgtg gtgagtcgct tgatcatgga aggtcgtgat attgaccttt acgcagggat
     5461 gaatcttcca atggtgattg aatttatcaa tgcgagcctt acaggcgcag atgcggacta
     5521 caagagccgt gctgcagaaa gcattgtgaa agttaatgac ctgttagcgg gcttcgatga
     5581 tgacgaagat gaataatact cttcgaaaat ctcttcaaac tacgtcaacg tcgccttgcc
     5641 gtaggtatat gttactgact tcgtcagtct tatccggcaa cctcaaaacg gtgttttgag
     5701 ctgacttcgt cagtcttatc cggcaacctc aaagcagtgc tttgagcagc ctgcggctag
     5761 tttcctacag attttagttg gaactcgatt caattcatgt gacaacgtga aaatcgttag
     5821 agcattttat atagaatata catgggaatg gagcttactc ccattcccat atttaataga
     5881 aaaagaggaa ctcaatgcta cattatacaa aagaagactt gctcgaattg ggtgcagaaa
     5941 tcactacgcg tgaaatctac caacagcctg atgtatggag agaagctttt gaattttatc
     6001 aagcaaaacg tgaagaaatt gcagcctttc tacaagaaat cgctgataaa catgactata
     6061 ttaaggttat cttgacaggt gctgggactt ctgcttatgt gggagatacc ttgctacctt
     6121 actttaagga agtctatgac gaacgcaaat ggaatttcaa tgctattgcg acaacagata
     6181 tcgttgccaa accagcaacc tatttgaaaa aagatgtggc aactgtcctt gtgtcttttg
     6241 ctcgtagtgg gaactcgcct gaaagtgtgg cgactgttga tttggccaaa tccttggtgg
     6301 atgagcttta tcaagtgacg attacttgtg cagcagatgg taaattggct cttcaagctc
     6361 acggtgatga ccgtaatctc ttgctcttgc aaccagctgt ctctaatgat gctggatttg
     6421 ccatgacttc tagctttacg tctatgatgt tgacagctct cttggtcttt gatcctacag
     6481 aatttgctgt taagtctgaa cgttttgaag ttgtatctag tcttgcccgt aaagttttag
     6541 acaaggtaga agatgtcaaa gagctcgttg atttagactt taaccgtgtc atctatctag
     6601 gcgctggtcc tttctttgga cttgctcatg aagctcagct caagattttg gaattaactg
     6661 ctggtcaagt tgcgaccatg tatgaaagcc cagttggctt ccgtcacggt ccaaaatctc
     6721 ttatcaacga caatacagtt gttttggtct ttggtacaac gacagcctac actcgtaagt
     6781 acgacttgga cttggttcgt gaagttgctg gtgaccagat tgctcgtcgt gttgtgcttt
     6841 tgagtgatca agcttttggt cttgaaaatg tcaaagaagt ggcccttggt tgtggcggtg
     6901 tcttgaatga tatttaccgt gtcttccctt acatcgttta tgcccaactc tttgccctat
     6961 tgacttcact caaggtagaa aataaaccag atacaccgtc tcctacaggt acagtaaacc
     7021 gtgtagtaca aggtgtcata attcacgaat atcaaaagta agacagtgtt tatgaattct
     7081 tgacaagagg atttgtaaat tatcagataa accatagatt gtcagtacgc tttctatggt
     7141 ttgtttgctt gagagaaata gtaaaaggag aacagaatga aagcatacac agagcgtgta
     7201 tttggaaatg ttgagggcga ggatgtcttg gcctatcgat ttgagacaga cggtggctac
     7261 caacttgagg ttatgactta tggcgcgacc atcttgcgct atgtcgcacc tgacaaggct
     7321 ggaaattttg ccaatgttat cttgggattt gatgactttg atagttatgt aggcaatagt
     7381 cccaagcatg gagcaagtgt aggtcctgta gcgggtcgta ttgcaggtgc gacctttgag
     7441 ctcaatggta agacctatga ccttgaggtt aataatgcta gcaactgtaa tcacagtggt
     7501 tcaactggtt gggattccag cttgtttgaa gttgaagaag taagcgatca tggcttgact
     7561 ctctacacag agcgtacaga tgggacagga gggttccctg gaaatctcaa gatttggatc
     7621 agttatcact tggaagaaac tggtgcctat gaaatcatct acaaggtaac gaccgatcag
     7681 gatacgctgg tcaatccaac caaccacagc tatttcaact tgtctggtga tttcacgcag
     7741 acgattgacc gtcatgtctt ccaactaaac acagagggca tttactcaat cgctcctgac
     7801 ggtgttcctg ccaaaactcc agaagccaac cgtgatgtgg tcaaacacat ctacaatggt
     7861 accttgttga aggatatctt tgcagaagaa gatgagcaaa tccagctggc atcaggtttg
     7921 gatcacccat ttgcccttcc tgcaggccat gacaatgctg gattccttta tgaccaaaat
     7981 tcaggtcgct tcctgctttt caagacagaa gctccttgct ttgtggtcta cacagcaaac
     8041 tttgtggatg aaagtgtcat cataggaggt cagccaatgc tacagcacaa tgggattgct
     8101 cttgaagcgc aagctttacc agatgccatt cacagtgacc ttaaaggcca agtcattctt
     8161 aaagctggtc aaaccttcac cagtaagaca cgttatgaac ttgttgtgaa ataaaaaaag
     8221 ctggtcaaaa agtctttaaa attaaaataa cgcatattgt caggtgatga aaagtttgat
     8281 aatatgcgtt ttattatgaa atgatttact ttatcttatc ctgaaatggt gttatttttg
     8341 agttatctgg cttgactttc ttgatggaaa ttatataata gttgtaataa caataattac
     8401 aacgagaaaa gaggaccatt tatgacctat gaatacaaga gtcacattta tttggcagag
     8461 acagttttaa atgtaaagga tttggccagt caaacagcct tttatcagca agttattggt
     8521 ttagaaatct tatctcaaac tgagacagag tccattctag gccttggtgg aaaagtcttg
     8581 gtacagctga ttcaagcaca agagagcgga gaagtgaggg aacattatgg tctttaccat
     8641 ttggccattc tcttgcccac acgcaaggct ttggctgatg tcttgaaaca cctgacagat
     8701 ttgcaaattc ctcttgtcgg cggtgcagac catggttaca gtgaagctat ttacttggaa
     8761 gatttggagg gaaatggcat tgaactctat cgagataagc cagtttccac atggaatatt
     8821 cgagaagatg gacgcattat cggagtgacg gaggctcttg cggcgcagga catttatgag
     8881 ttgggggaaa gagtagagcc ctttatcctg gcagaaggta caagaatggg gcatattcat
     8941 ctttccgtca aggatagtcg aaagtccagc cagttttatc aaaaggtgtt agggctagag
     9001 aataaattca gtgtgcctgg cgctagttgg attgcggctg gagattacca tcatcattta
     9061 gcagttaacg aatggggagg aaaaggtctg gcttcgcgta agcaaggctt accaggttta
     9121 gcctactatg tcatcgaagt cgcacacaaa aaagaactat taacgattgc ccaacgagca
     9181 caagaagttg acgcaccaat caaatggatg acatcgagcc aattggaaat cacagactca
     9241 gatggcatcg tgacccgtat tcgtttagca aggtaaaatt aggaattttt aataagttat
     9301 aagtattgct agtttgcttt ttgaacggtc tttggtaaca gaactgcgtg agctggatgg
     9361 gtgagaattt tgacaaagaa gctgattaga gatataatga agaaaaatcg agcaaggaaa
     9421 aagaaatgac aaattcaaag tatattactt gtttgaaacg ttcagagggc cagttgtgtg
     9481 ggattcaaaa gatgattgaa ggagattgtg actgtgctga tattgtgacg caactgacag
     9541 cagtgagatc tagtgtagag cgcgtgattg agatgataat taccgaaaat cttactgaat
     9601 gtatcaatca gcccctagat gattctgaag ctcaaaagga acgcctagaa aaggctatcc
     9661 gatacttgat taaacggaaa taaatgaatg ttttgagacg atatgtgcaa aggtctatta
     9721 actattagaa tagtttaatg tgagtcaaaa gaagttgtaa aaaatcttta aaattaaaaa
     9781 aacgcatagt atcaggtgtt gaatgtactg accccaaaaa gttagatttt ttctgtctaa
     9841 cttttggggt gcagttcata agaaccttgg taatatgcgt tttattatgt agagacttat
     9901 actcttcgaa aatctcttca aaccacgtca gtttcacctt gccgtagata tggttactga
     9961 cttcgtcagt tctatctaca acctcaaaac agtgttttga gctgacttcg tcagttccat
    10021 ccacaacctt aaaacagtgt tttgagcagc ctgcggctaa cttcctagtt tgcactttaa
    10081 ttttcattga gtattacttc atcttctcct gaaattgagt ttttgcccag ctcctcttga
    10141 ttattctact tgaccgggcc aagcattcat accaccttct acgttggtaa cggtgaggcc
    10201 ttgggagctg agaaattggc aggtagatgc agagcggact cctccttgac aaataacata
    10261 gtattcatgg tcaggtttga gttctttgta gccttgctct aaggtactta atggaagatt
    10321 tttggcacct ggtgcatgtc ctgcttggaa ttcatgtgct tcacggacat cgataagttc
    10381 tagattttca ttttggtatt ttccataaaa gtcagccatg ctgatactag tttccatatt
    10441 ctactccttc tgggttaacc attttgtata gtgaataagc gccgtcaagg ttttgggcgg
    10501 taaatcctgc ttgcttgagg ataggctctg cgatatagct gcgcaaacca ctgtggcagc
    10561 tgacgatgta ggcttgtttt ttgtccagtt catcagcttc ctccttgaca ctttgtcagt
    10621 ttttgataca atagtacaaa attagaggag gcaggctatg attcagaaac atgcgattcc
    10681 tattttagag tttgatgaca atcctcaggc ggttatcatg cccaatcacg aggggttgga
    10741 cttgcagttg ccaaagaagt gtgtctatgc ttttttaggt gaggagattg accgctatgc
    10801 gagggaagta ggggcgaact gtgttggcga attcgtttct gctaccaaga cctatccagt
    10861 ctatgtcatc aactacaagg gtgaggaggt ctgtctggct caggctcctg ttggctccgc
    10921 tccagcagcc cagtttatgg attggttgat tggctatggt gtggagcaga ttatctctac
    10981 tgggacctgt ggtgtcctag ctgatataga ggaaaatgcc tttctagtcc ctgttcgcgc
    11041 tttgcgagat gagggagcca gttaccacta tgtggcacct tgtcgttata tggaaatgca
    11101 gccagaggct attgctgcta ttgaggaagt tttggaagac agagggattc cttatgaaga
    11161 agtcatgacc tggacgacag acggttttta ccgagaaacg gctgaaaagg tggcttatcg
    11221 taaggaagaa ggctgtgctg ttgtggagat ggagtgttct gctcttgcgg cagtagctca
    11281 attgcgtggg gttctctggg gtgaattgtt gttcacagca gattctctag cggacttgga
    11341 ccagtacgac agtcgtgact ggggctcgga agcttttaat aaggcgctag aactgagttt
    11401 agcaagtgtt caccaccttt agttgtacag gcaaaggatt tgttttatca taaaatgtct
    11461 agatcatact tttcaaaaat atgtttaaac gaggtcacct tcctcttgtc ctaggcatgt
    11521 tgaggttggg aaaaatcttt aaaatcagaa aaacgtatca tatcaggtga tgaaaatttt
    11581 gatactatgc gttttatgtt ggtaagattt agagtgagat gaaatgatac tcttcgaaaa
    11641 tctcttcaaa ccaggtcagc ttcaccttgc cgtatagatg ttactgactt cgtcagttct
    11701 atctgcaacc tcaaaacagt gttttgagct gacttcgtca gttctatcca caacctcaaa
    11761 acagtgtttt gagcaacctg tgactagctt tctaagcgat gccttggttc tcattgccta
    11821 taatcaaaaa gagaaatttt ctcctgaaaa gcatatagag tagctggcgt taaaagctcc
    11881 tgtcttgctt ttttgatcta tagtcacatc tatcaagtat tgttcttgcc taagctatca
    11941 ataaaaaggt ggcatttttt aggcttggtg ttagtgaatt ttgccttatc ctatctaagt
    12001 catttcgagc tttttatggt acaatggaaa catgttattc aaattatcta aggaaaaaat
    12061 agagctaggc ttatctcgtt tatcgccagc ccgtcgtatt tttttgagtt ttgccttggt
    12121 cattttacta ggctctcttc ttttgagctt gccctttgtc caagttgaaa gctcacgagc
    12181 gacttatttt gatcatcttt tcactgctgt ctctgcagtc tgtgtgacag gtctctcaac
    12241 ccttccagta gctcacacct ataatatctg gggccaaata atctgtttgc tcttgattca
    12301 gatcggtggt ctagggctca tgacttttat tggggttttc tatatccaga gcaagcagaa
    12361 acttagtctt cgtagccgtg caactattca ggatagtttt agttatggag aaactcgatc
    12421 tttgagaaag tttgtctatt ctatttttct cacgaccttt ttggttgaga gcttgggagc
    12481 tattttgctt agttttcgcc ttattcctca acttggctgg ggacgtggtc tttttagttc
    12541 catttttcta gcgatctcag ccttctgtaa tgccggtttt gataatttag ggagcaccag
    12601 tttatttgct tttcagaccg atttactggt caatctggtg attgcaggct tgattattac
    12661 aggcggcctt ggttttatgg tctggtttga tttggctggt catgtaggaa gaaagaaaaa
    12721 aggacgtctg cactttcata cgaagcttgt actattattg actataggtt tgttgttatt
    12781 tggaacagca actactctct ttcttgagtg gaacaatgct ggaacgattg gcaatctccc
    12841 tgttgccgat aaggttttag ttagcttttt tcaaacagtg acgatgcgaa cagctggctt
    12901 ttctacgata gattatactc aggctcatcc tgtgactctt ttgatttata tcttacagat
    12961 gtttctaggt ggggcacctg gaggaacagc tgggggactc aagattacga cattttttgt
    13021 cctcttggtc tttgcacgaa gtgagcttct aggcttgcct catgccaatg ttgcgagacg
    13081 aacgatcgcg ccgcgaacgg ttcaaaaatc ctttagtgtc tttattatct ttttgatgag
    13141 cttcttgata ggattgattc tgctagggat aacagccaaa ggcaatcctc cctttatcca
    13201 cctcgtattt gaaaccattt cagctcttag tacagttggt gtaacggcaa atctgactcc
    13261 tgaccttggg aaattggctc tcagtgttat catgccactt atgtttatgg gacgaattgg
    13321 tcccttgacc ttgtttgtta gcttggcaga ttaccatcca gaaaagaaag atatgattca
    13381 ctatatgaaa gcagatatta gtattggtta agaaaggaaa gagcatgtca gatcgtacga
    13441 ttggaatttt gggcttggga atttttggga gcagtgtcct agctgcccta gccaagcagg
    13501 atatgaatat tatcgctatt gatgaccacg cagagcgcat caatcagttt gagccagttt
    13561 tggcgcgtgg agtgattggt gacatcacag atgaagaatt attgagatca gcagggattg
    13621 atacctgcga taccgttgta gtcgcgacag gtgaaaatct ggagtcgagt gtgcttgcgg
    13681 ttatgcactg taagagtttg ggggtaccga ctgttattgc taaggtcaaa agtcagaccg
    13741 ctaagaaagt gctagaaaag attggagctg actcggttat ctcgccagag tatgaaatgg
    13801 ggcagtctct agcacagacc attcttttcc ataatagtgt tgatgtcttt cagttggata
    13861 aaaatgtgtc tatcgtggag atgaaaattc ctcagtcttg ggcaggtcaa agtctgagta
    13921 aattagacct ccgtggcaaa tacaatctga atattttggg tttccgagag caggaaaatt
    13981 ccccattgga tgttgaattt ggaccagatg acctcttgaa agcagatacc tatattttgg
    14041 cagtcatcaa caaccagtat ttggataccc tagtagcatt gaattcgtaa agagggatga
    14101 cccctctttt ttgatgccta agatggcaaa tagagacaga agccccttgt cttctagtaa
    14161 aagttcttca aaggctggac tttatggtaa aatagaaaga agtgacaaga gagagtaata
    14221 ctcaatgaaa atcaaagatc aaactaggaa actagctacg ggctgctcaa aacactgttt
    14281 tgaggttgca gatagaactg acgaagtcag taacatctat acggcaaggc gacgttgacg
    14341 cggtttgaag agattttcga agagtataag aaaaaatcag tcccctaaag gagtagatta
    14401 tgaagttatt gtctatcgca atttctagct ataatgcagc agcctatctt cattactgtg
    14461 tggagtcgct agtgattggt ggtgagcaag ttgggatttt gattatcaat gacgggtctc
    14521 aggatcagac tcaggaaatc gctgagtgtt tagctagcaa gtatcctaat atcgttagag
    14581 ccatctatca ggaaaataaa tgccatggcg gtgcggtcaa tcgtggcttg gcagaggctt
    14641 ctgggcgcta ttttaaagta gttgacagtg atgactgggt ggatcctcgt gcctacttga
    14701 aaattcttga aaccttgcag gaacttgaga gcaaaggtca agaggtggat gtctttgtga
    14761 ccaattttgt ctatgaaaag gaagggcagt ctcgtaagaa gagtatgagt tacgattcag
    14821 tcttgcctgt tcggcagatt tttggctggg accaggtcgg aaatttctcc aaaggccagt
    14881 ataccatgat gcactcgctg atttatcgga cagatttgtt gcgtgctagc cagttctaac
    14941 tgcctgaaca tactttttat gtcgataatc tctttgtctt tacgcccctt cagcaggtca
    15001 agaccatgta ctatctgcct gtcgatttct atcgttattt gattgggcgt gaggaccagt
    15061 ctgtcaatga gcaagtgatg attaagtgca ttgaccagca actcaaggtc aatcgactct
    15121 tgatagacca acttgatttg tcccaagtga gtcatcccaa aatgcgagaa tatctgctga
    15181 atcatattga actcacgacg gtgatttcca gtaccctgct caaccgatct ggaacagcgg
    15241 agcatctggc aaaaaaacgc caattgtgga cctatattca gcagaaaaat ccagaagtct
    15301 ttcaggctat tcgtaagacc atgttgagct gtttgaccaa acattctgtc ttgccagatc
    15361 gcaaactgtc caatgtcgtc tatcaaatca ccaaatctgt ttatggattt aattaatata
    15421 agtgttttat aagagggatt taagaaaaat tttaactttt tcttagtcct ttttaatttc
    15481 aggagattat actagagtca tcaaataaag aaagactcta aggagaatcc tatgaaattc
    15541 aatccaaatc aaagatatac tcgttggtct attcgccgtc tcagtgtcgg tgttgcctca
    15601 gttgttgtgg ctagtggctt ctttgtccta gttggtcagc caagttctgt acgtgccgat
    15661 gtggtcaatc cgaccccagg tcaagtctta cctgaagaga catcgggaac gaaagagggt
    15721 gacttatcag aaaaaccagg agacaccgtt ctcactcaag cgaaacttga gggcgttact
    15781 ggaaatacga attcacttcc gacacctaca gaaagaactg aagtgagcga ggaaacaaac
    15841 tcttctagtc tggatacact ttttgaaaaa gatgaagaag ctcaaaaaaa tccagagcta
    15901 acagatgtct taaaagaaac tgtagataca gctgatgtgg atgggacaca agcaagtcca
    15961 gcagaaacta ctcctgaaca agtaaaaggt ggagtgaaag aaaatacaaa agacagcatc
    16021 gatgttcctg ctgcttatct tgaaaaagct gaagggaaag gtcctttcac tgccggtgta
    16081 aaccaagtaa ttccttatga actattcgct ggtgatggta tgttaactcg tctattacta
    16141 aaagcttcgg ataatgctcc ttggtctgac aatggtactg ctaaaaatcc tgctttacct
    16201 cctcttgaag gattaacaaa agggaaatac ttctatgaag tagacttaaa tggcaatact
    16261 gttggtaaac aaggtcaagc tttaattgat caacttcgcg ctaatggtac tcaaacttat
    16321 aaagctactg ttaaagttta cggaaataaa gacggtaaag ctgacttgac taatctagtt
    16381 gctactaaaa atgtagacat caacatcaat ggattagttg ctaaagaaac tgtcgaaaaa
    16441 gctgttaaag ataacgttaa
//
LOCUS       DBERDK010000042        16403 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.042, whole genome shotgun sequence.
ACCESSION   DBERDK010000042 DBERDK010000000
VERSION     DBERDK010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 16403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16403
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.042"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            85..399
                     /locus_tag="ACL73L_001365"
     CDS             85..399
                     /locus_tag="ACL73L_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003037418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HGS0772329.1"
                     /translation="MAKALIICAAGMSSSLMAQKTTEFLKGKGKDIVVDAISATEGGK
                     AIAAAAFDLYLVSPQTKMYFKQFEEAGSKAGKPVVQIPPQAYIPIPMGIEKMANLILE
                     NI"
     gene            517..2496
                     /locus_tag="ACL73L_001366"
     CDS             517..2496
                     /locus_tag="ACL73L_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HGS0772330.1"
                     /translation="MNVLNIKEKDILQFLIKNKERFVTSKELAEYLSCSDRTVRNVLK
                     LIEKTMIIQGVRLISKQGQGYQIFFENQGAYQEFRQTYELEEDYTKTAVSKGDDRLVF
                     ILNKLLFEQVPVLFDDLADELYVSRSTLSHDFRKIRVMLSEYNLSIESRANKGVYVSG
                     EERDKRRFIINYFLENQFFKTIHCYVKFNFFDQTVPLEEFARIVLDECQEANLKLSDF
                     VLQNLVVHIALSVIRLKSGFEIKNIDCQMTDDAIERKVAQRILSKVREVTNQEFPVQE
                     IDYITLHLLAKSQQCQKNQKNISEEVLKKSLLKTFQNLGLDDMYNFSSDFQLIEGLIT
                     HLMTLQVRLESRITLNNPLVDEIKQNYSDIFFMTREILANMDKFLEWTISDDEVAYVS
                     LHFLAAMERSKESTKFNILAICATGFGAAQMLRNRLETEFGKRVEVVDVIGYYELNQE
                     KLKGIDFIVSAVDLSNLYFQIPVFKVSVFLKSDEMEMIRKAMDQMQVSSYVQSSKINK
                     FENNGFRQYFSKENFLICTESDKVNLLEKMVESLSVGESNEFEQSLLYGIKQREELSS
                     VVFSEKIAVPHPIQSFGTEGKVSVAICKDSLLWDNQSSHVQLVFLLSPSIYGNEGLAT
                     VTKKIVSLTENDELQNQLISCNNFEDFINIFEKIK"
     gene            2506..2820
                     /locus_tag="ACL73L_001367"
     CDS             2506..2820
                     /locus_tag="ACL73L_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HGS0772331.1"
                     /translation="MNQEELQVAAFEIILHSGNARSEIHEAFAKMREGSFDDAESKLN
                     QSNEIILEAHHAQTKLLQEYASGVEIKIEIIMVHAQDHLMTTMTLLEVAKEMLALYKK
                     VN"
     gene            2851..3357
                     /locus_tag="ACL73L_001368"
     CDS             2851..3357
                     /locus_tag="ACL73L_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772332.1"
                     /translation="MDKKERQKIEQQRREMALTNTFFNRYLLLRYSIALFFFGNIYWL
                     LSQFISPSPIIIFPIMLIVFSILATVEQFKLYGNRKEKLGITLMFVRIQMLISIGLLV
                     LTWTSWFKNLFPIFENNQVARLFVFVVLLLGLVLSLLDIRRIKKIYKRTDKAYQQFVQ
                     LEKNSLSL"
     gene            3437..4783
                     /gene="celB"
                     /locus_tag="ACL73L_001369"
     CDS             3437..4783
                     /gene="celB"
                     /locus_tag="ACL73L_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HGS0772333.1"
                     /translation="MDQQNGLFGFLENHVMGPMGKLAQFKVVRAITAAGMAAVPFTIV
                     GSMFLVFSILPQAFSFWPIVADIFSASFDKFTSLYMVANYATMGSLSLYFVLSLAYEL
                     TKIYAEEEELNMNPLNGALLALMAFVMTVPQIIFDGGMMKTVTSLKEGAVIADGWAMG
                     NGVARFGTTGIFTAIIMAIVTVLIYRMCVKHNWVIKMPEAVPEGVSRGFTALVPGFVV
                     AFVVIFINGLLVAMGTDIFKVIAIPFGFVSNLTNSWIGLMIIYLLTQLLWIVGIHGAN
                     IVFAFVSPIALANMAENAAGGHFAVAGEFSNMFVIAGGSGATLGLCLYIAFASKSEQL
                     KAIGRASVVPALFNINEPLIFGLPIIYNPALAIPFILAPMVTATIYYVANSLNFIKPI
                     IAQVPWPTPVGIGAFLGTADLRAVLVALVCAFAAFLVYLPFIRVYDQKLVKEEQGI"
     gene            5162..5338
                     /locus_tag="ACL73L_001370"
     CDS             5162..5338
                     /locus_tag="ACL73L_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772334.1"
                     /translation="MSLDIDKEKMTIMGIAFENRSVFKSVWYALSTNMIEGWRPTVSD
                     VEKLRDEALALGMT"
     gene            5740..7953
                     /locus_tag="ACL73L_001371"
     CDS             5740..7953
                     /locus_tag="ACL73L_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HGS0772335.1"
                     /translation="MKIFKGEFYRISVLTDKLVRLEYSQTGSFEDRTTQLIYNRDFGQ
                     ISLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIELKGNFAVYGSRWYFGESIETLK
                     GTARTLDKADGAISLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
                     DYRGAIRDFYHLTGSTPLLPRYALGNWWSRYWPYTSDEYLDLIDRFETEKIPLSIGVL
                     DMDWHITDIPARFGSGWTGYSWNRNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYE
                     EAYPQIAKRLGLNVELEEPAIFDFFNPSFREAYFKDVHYELEKQGVDFWWIDWQQGTQ
                     GMLDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTIISWNSLRFQPY
                     FTATASNIGYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPW
                     FFSETTSKIMKKYLRLRHQMIPYLYTMNVQTHEEGAPLISPIYYFYPENDESYNVPNQ
                     YFFGTELMVAPIVEKMDLTFQSAKVDVWFPEGEWYDFFSEKKYTGGVKLSVYRDISTT
                     PVFAKSGAIIPLVGSEIGMGVDLPEVVDWYVFPGKQHSFEMLEDQNGQRYKTRLSIDW
                     EMGMVELALQGDSSIVPSNRKHRIHFKGTNVSIIELPNKNDTAKFEWKDNKRTSLNDE
                     VFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQDKELRGRLLEMIFTSQN"
     gene            complement(8175..8651)
                     /locus_tag="ACL73L_001372"
     CDS             complement(8175..8651)
                     /locus_tag="ACL73L_001372"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HGS0772336.1"
                     /translation="MTSLYDFSVLNQNNQATPLDSYRGKVLLIVNTATGCGLTPQYQG
                     LQELYERYQDQGFEILDFPCNQFMGQAPGSAEEINAFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLQDQKSGPLGKRVEWNFAKFLIGRDGQVFERFSSKTDPKQIEEAIQTLL"
     gene            8874..12074
                     /locus_tag="ACL73L_001373"
     CDS             8874..12074
                     /locus_tag="ACL73L_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored hyaluronate lyase"
                     /protein_id="HGS0772337.1"
                     /translation="MQTKTKKLIVSLSSLVLSGFLLNHYMTIGAEETTTNTIQQSQKE
                     VQYQQRDTKNLVENGDFGQTEDGSSPWTGSKAQGWSAWVDQKNSADASTRVIEAKDGA
                     ITISSHEKLRAALHRMVPIEAKKKYKLRFKIKTDNKIGIAKVRIIEESGKDKRLWNSA
                     TTSGTKDWQTIEADYSPTLDVDKIKLELFYETGTGTVSFKDIELVEVADQLSEDSQTD
                     KQLEEKIDLPIGKKHVFSLADYTYKVENPDVASVKNGILEPLKEGTTNVIVSKDGKEV
                     KKIPLKILASVKDAYTARLDDWNGIIAGNQYYDSKNEQMAKLNQELEGKVADSLSSIS
                     SQADRTYLWEKFSNYKMSANLTATYRKLEEMAKQVTNPSSRYYQDETVVRTVRDSMEW
                     LHKYVYNSEKSIVGNWWDYEIGTPRAINNTLSLMKEYFSDEEIKKYTDVIEKFVPDPE
                     HFRKTTDNPFKALGGNLVDMGRVKVIAGLLRKDDQEISSTIRSIEQVFKLVEQGEGFY
                     QDGSYIDHTNVAYTGAYGNVLIDGLSQLLPVIQKTKSPIDKDKMQTMYHWIDKSFAPL
                     LVNGELMDMSRGRSISRANSEGHVAAVEVLRGIHRIADMSEGETKQRLQSLVKTIVQS
                     DSYYDVFKNLKTYKDISLMQSLLSDAGVASVPRTSYLSAFNKMDKTAMYNAEKGFGFG
                     LSLFSSRTLNYEHMNKENKHGWYTSDGMFYLYNGDLSHYSDGYWPTVNPYKMPGTTET
                     DAKRADSDTGKVLPSAFVGTSKLDDANATATMDFTNWNQTLTAHKSWFMLKDKIAFLG
                     SNIQNTSTDTAATTIDQRKLESSNPYKVYVNDKEASLTEQEKDYPETQSVFLESSDSK
                     KNIGYFFFKKSSISMSKALQKGAWKDINEGQSDKEVENEFLTISQAHKQNGDSYGYML
                     IPNVDRATFNQMIKELESSLIENNETLQSVYDAKQGVWGIVKYDDSVSTISNQFQVLK
                     RGVYTIRKEGDEYKIAYYNPETQESAPDQEVFKKLEQAAQPQVQNSKEKEKSEEEKNH
                     SDQKNLPQTGEGQSILASLGFLLLGAFYLFRRGKNN"
     gene            12454..12830
                     /gene="tnpA"
                     /locus_tag="ACL73L_001374"
                     /pseudo
     CDS             12454..12830
                     /gene="tnpA"
                     /locus_tag="ACL73L_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002458050.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            13012..13074
                     /locus_tag="ACL73L_001375"
                     /pseudo
     CDS             13012..13074
                     /locus_tag="ACL73L_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596946.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(13218..13847)
                     /locus_tag="ACL73L_001376"
     CDS             complement(13218..13847)
                     /locus_tag="ACL73L_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HGS0772338.1"
                     /translation="MTKSDTIIELKKQKIVAVIRGNTKEEGLQASIACIKGGIKAIEI
                     AYTNQYAGQIIKELVDLYQDDQSVCIGAGTVLDAVTARDAILAGANYVVSPSFHAETA
                     KMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPGSTLSPAYISAVKAPIPQVSVMV
                     TGGVGLNNIPQWFAAGADAVGIGGELNKLASQGNFDRISEIAQQYVTLR"
     gene            complement(13857..14858)
                     /locus_tag="ACL73L_001377"
     CDS             complement(13857..14858)
                     /locus_tag="ACL73L_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HGS0772339.1"
                     /translation="MTKILLFGEPLIRISPLDATSIGDHVASSTYFGGSEINIACNLQ
                     ALGISTKVFTALPANEIGDRFLTFLKQHQIDTSSICRLGDRIGLYYLENGFGCRQSEV
                     FYDRKHTSISQIRPNMLDMDSLFQGISHFHFSGITIAIGQEVRAILLLLLEEAKRRGI
                     VVSMDLNLRTKMISVLEAKYEFSKFARFTDYCFGIDPLMIDDQNLEMFPRDSASLEEV
                     ENRMRLLKEAYGFKAIFHTLRSSDEQDKNVYQAYALEERFEESVQLKTAVYQRIGSGD
                     AFISGALYQLLHHSSLKTTIDFAVASATLKCTLPGDHLSTSATSIENLLANAQDIIR"
     gene            complement(14889..15530)
                     /locus_tag="ACL73L_001378"
     CDS             complement(14889..15530)
                     /locus_tag="ACL73L_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HGS0772340.1"
                     /translation="MKIALINENSQASKNHIIYDSLKEATDKKDYQLFNYGMRGEEGE
                     SQLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGVVCGLAVDPTDAYLY
                     SQINGGNALSIPYAKGFGWGAELTLKLMFERLFAEEMGGGYPRERVIPEQRNARILNE
                     VKQITHNDLMTILKTIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEVLAK"
     gene            complement(15549..16364)
                     /locus_tag="ACL73L_001379"
     CDS             complement(15549..16364)
                     /locus_tag="ACL73L_001379"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HGS0772341.1"
                     /translation="MTNTSFSIEQFSLKGKIALITGASYGIGFAIAKSYAEAGATIVF
                     NDINQDLVNKGIEAYREVGIEAHGYVCDVTDEDGIQAMVKQIEQEVGVIDILVNNAGI
                     IRRVPMCEMSAADFRKVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRET
                     VSAYAAAKGGLKMLTRNIASEYGGANIQCNGIGPGYIATPQTAPLRELQEDGSRHPFD
                     QFIIAKTPAARWGNPEDLMGPAVFLASDASNFVNGHILYVDGGILAYIGKQPE"
BASE COUNT         5279 a         2668 c         3407 g         5049 t
ORIGIN      
        1 acgctttatt ttataataaa aatagataac aaataagcca acttagcaca gctttgattg
       61 gatttaagaa aaggagaaaa tattatggca aaagctttaa ttatttgtgc agcaggaatg
      121 tcttcatcac ttatggcaca gaaaacaaca gagtttctaa aaggaaaagg gaaagatatt
      181 gtagttgatg ctattagtgc tactgaagga gggaaagcta tcgcagcagc agcttttgat
      241 ttgtatcttg tcagtcctca aacaaaaatg tactttaagc aatttgaaga ggctggttct
      301 aaagcaggaa aaccagttgt acaaatacct cctcaagcct atattcctat tccaatggga
      361 attgaaaaaa tggctaattt gattcttgag aatatttgat gaacacatga aatgtcaata
      421 aatatttaag ttaagataaa ttgttttgag tttgatataa attgatttaa gtgtataata
      481 tgcttcaata tatgaaaaca tacgttttag gagggaatga atgtgttaaa cattaaagag
      541 aaagatatcc ttcaattttt aataaaaaat aaggaacgtt ttgttacaag caaagaactg
      601 gcagaatatt tatcctgttc agatagaact gtccgtaatg tactgaaatt aattgagaag
      661 acaatgatta ttcaaggagt ccggttgatt tcaaaacaag gtcaagggta tcaaatattt
      721 tttgagaatc aaggcgctta ccaggagttt agacaaactt atgaactgga agaagactat
      781 acgaaaactg cggtttcaaa aggtgatgat cgtttagtat ttatcttaaa taaattatta
      841 tttgagcaag ttccagttct ttttgatgat ttagctgatg aactttacgt tagccgttca
      901 acattatctc atgatttcag aaagatacga gtgatgttat cagagtataa tttgtccatt
      961 gagagtaggg cgaataaagg agtttatgtc tcaggcgagg aacgtgataa acgacgtttt
     1021 atcataaatt atttcctaga gaatcaattt tttaaaacaa tacattgcta tgtaaaattt
     1081 aacttttttg atcaaactgt tccattagaa gaatttgcta gaattgtttt ggatgaatgc
     1141 caagaggcca acctaaaact atctgatttt gtactacaga atttagtagt tcacatagca
     1201 ttgtctgtga ttcgtttaaa atccggattt gaaataaaaa atatagactg tcagatgact
     1261 gatgatgcaa tagagagaaa ggttgcccaa agaattcttt ctaaagtaag agaagtaaca
     1321 aatcaggaat ttccagttca ggaaatagac tacattactt tgcatttgtt agctaagagt
     1381 caacaatgtc aaaaaaatca gaagaatatt tctgaagaag ttttaaaaaa atctttactt
     1441 aaaacatttc agaatttagg attagatgat atgtataact tttcttcaga tttccaacta
     1501 attgaaggat tgataactca tctaatgaca ctacaagtac gtttagaaag ccggattacc
     1561 ttaaataacc ctttggtaga tgaaattaaa cagaattata gtgatatatt ctttatgact
     1621 agagaaattc ttgcaaatat ggacaagttc ttggagtgga caatatcaga tgatgaagta
     1681 gcatatgtaa gtcttcactt tttagctgct atggaacgta gcaaagagag tactaagttt
     1741 aatattcttg ctatttgtgc aacaggattt ggtgcagccc aaatgttaag aaatcgtttg
     1801 gaaacggagt ttgggaagcg agtagaagtt gttgatgtaa taggctatta tgaattgaat
     1861 caagaaaaac ttaaaggaat agattttata gtgtcagctg ttgatttatc aaatctatat
     1921 tttcaaatcc cagtttttaa agttagtgtg tttcttaaga gtgatgagat ggaaatgatt
     1981 agaaaagcta tggatcagat gcaagtttct agttatgtac aatcatcaaa gattaacaaa
     2041 tttgaaaaca acggctttag acagtatttt agtaaagaaa actttttaat atgtactgaa
     2101 tcagacaagg taaatttact tgaaaagatg gttgagagtt tatctgttgg tgaatcaaat
     2161 gagtttgaac aatctttact ttatggaatt aaacagcgcg aagaattaag ttcagttgtt
     2221 ttttctgaaa aaattgcagt tccgcatcct attcagtcct ttggtacaga gggaaaggtt
     2281 tcggttgcaa tttgtaaaga ttcgttactc tgggataatc aaagttcaca tgtccagcta
     2341 gtatttcttc tatctccatc aatatatggg aacgaaggtt tggcaactgt gactaaaaag
     2401 atagtctctc tgacagagaa tgatgaattg caaaatcagc taatatcttg caataacttt
     2461 gaagatttta ttaacatatt tgagaagata aaataggagg ttcatatgaa tcaagaggaa
     2521 ctgcaggttg cagcatttga aattatcctg cattcgggaa atgcaaggtc agaaatacat
     2581 gaagcatttg caaaaatgag agaaggtagt tttgatgatg cagaaagtaa attaaatcaa
     2641 tcaaatgaga taattcttga agcgcatcat gcacaaacca aactcttgca agaatacgca
     2701 agtggagtgg aaattaaaat tgaaattatc atggtacatg ctcaagatca tttaatgacc
     2761 actatgacat tattagaagt agcaaaggaa atgttagctc tttataagaa agttaactaa
     2821 atatgtgttg ttatataagg aggaaatgat atggataaaa aagaacgaca aaagattgaa
     2881 caacaacgtc gagagatggc tctaactaat acttttttca atcgatatct tcttctacgt
     2941 tattcaatag cactcttctt ttttgggaat atttattggc ttttgagtca gtttattagt
     3001 ccctcgccta ttattatttt ccccattatg ctcatagtat tttctatttt agcaactgtt
     3061 gaacaattta aactctatgg aaataggaaa gaaaagttgg gaataacact aatgtttgtt
     3121 agaatacaaa tgcttatttc aataggatta cttgttttaa cttggacaag ttggtttaag
     3181 aatctttttc cgatttttga aaataaccaa gtagcacgtt tatttgtttt tgtagttctt
     3241 ctattaggtt tagttctgag tttgttagac attagaagaa ttaaaaaaat ttataaacga
     3301 acagataagg cttatcaaca atttgtccaa ttggaaaaga actcacttag tttataatcc
     3361 tgatctagta taatttgtat ttgaaactag tctttagaaa gtaaaaatta ttatgtttta
     3421 ttaaggaggt ctatttatgg atcaacaaaa cgggttgttt ggttttcttg aaaaccatgt
     3481 tatgggacca atgggcaaac ttgctcagtt taaagtagta cgtgctatca cggctgcagg
     3541 tatggctgct gtaccattta ctattgtagg atcaatgttt ttggtattca gtattttgcc
     3601 acaagctttc tcattttggc caattgtggc agatattttc tctgcttcat ttgataaatt
     3661 cacatcactt tacatggttg caaactatgc gactatgggt tctctatctc tttatttcgt
     3721 tctatcactt gcatatgaat tgacaaaaat ttatgcagag gaggaagaac tcaatatgaa
     3781 tcctcttaat ggtgccttgc ttgccttgat ggcttttgtc atgacagtac cgcaaatcat
     3841 ttttgatggt ggaatgatga agactgtgac aagtctaaaa gaaggagcag taattgcaga
     3901 tggatgggca atgggaaatg gagtcgcacg ttttgggaca acagggattt ttaccgcaat
     3961 cattatggca attgtgactg ttcttattta tcgtatgtgt gttaaacata attgggttat
     4021 taaaatgcct gaagctgttc cagaaggagt ttctcgtgga tttaccgctt tggttccggg
     4081 atttgttgtt gcatttgttg ttatctttat caacggtctt cttgtagcaa tgggaacaga
     4141 tatttttaaa gtcattgcaa ttccatttgg ttttgtatcc aatctgacta attcgtggat
     4201 tggtttaatg attatttatc tattgactca actactttgg attgtaggta tccacggtgc
     4261 gaacattgtt tttgcatttg ttagtccaat tgctcttgct aacatggctg aaaatgctgc
     4321 tggcgggcac ttcgctgttg caggtgaatt ttctaatatg tttgtaattg caggtggttc
     4381 tggtgcaact ttaggactat gtttatatat tgcttttgcc tctaaatctg aacagcttaa
     4441 agcaatagga cgagcatctg tagttccagc cttatttaat attaatgaac cattaatttt
     4501 tggattacct attatctata atccagcctt ggctatacca tttattttag caccaatggt
     4561 tactgctact atttattacg tagcgaattc tctaaacttt attaagccaa ttatcgcaca
     4621 ggttccatgg ccaactccag tagggattgg agctttctta gggacagcag atcttcgagc
     4681 tgtattagtt gctctagtat gtgcatttgc agcattctta gtctatcttc cattcatccg
     4741 tgtatatgat caaaaattgg tgaaagaaga gcaaggtatc taataagtaa aaaagtatac
     4801 tcgatgatag aatccatata gtttaaataa tgagcgttaa cattagcagt gaagaggtct
     4861 ttttaagata ttcttctctg ctttttcata ttttttcgct atgtagggta tgaagcaaaa
     4921 tagaaagtag ttcgaatagt tgataaacag ggaattgctg ttatttaaaa aaacaataaa
     4981 gtaaagtatg ttatcgtaga atacgataca atagtaaaaa agcaaaaaga atgcgtttta
     5041 tatttgggtt tgaggtttat aaaataggat aagcgtgctg aaaacttaga tatgacaaac
     5101 aaaataaaat ttttaaaagc tacagagaaa catggtataa tgtatctaaa ggaggtagca
     5161 tatgtcctta gatatagata aagaaaagat gacaatcatg ggaatagctt ttgaaaaccg
     5221 atccgttttt aagagtgttt ggtatgcttt aagtacaaat atgattgaag gatggcgacc
     5281 tacggttagt gatgttgaaa aattacgaga tgaagctctt gctctgggga tgacttaatg
     5341 aagcgcaact gttatgagtc ttatgactat gttgatccgg ataatttata taccgatccg
     5401 ggttcttcag ttctaagaaa taagcaggaa ggaaaaaagc acgcgagctt gaatatcgta
     5461 tgattgctag taagagtttg aaattattta tcaatcctat cttagttaat agtttaaaag
     5521 tagtttgttt cacaactgca taagcttaga aaagttgaaa agtgataact ctttaataaa
     5581 ctcttggttt gttaaaagat attttgtcca ctacattttt ttcaattgac tattatttgt
     5641 catagatata acagatagaa cattgcattc agaataatga aaaacgcatc aaaaatggtg
     5701 cgttctgttt ttatgctacc ataaaaggag gaaaacaaat tgaaaatttt taagggagag
     5761 ttttatcgaa tctctgtatt aacagacaag ctagtaaggt tagaatactc tcaaactgga
     5821 agttttgagg atagaacgac acaacttatc tataatagag attttggcca aatttcgtta
     5881 gattatatcg agacatcaaa cgtactagat attatgacgg actattttca tctgcacttt
     5941 aataaaggag aatttaacgc cgaaaattta tttatagaat taaaaggaaa ttttgccgta
     6001 tatggtagtc gctggtattt tggtgaatct attgaaacgt taaaaggaac agctcggact
     6061 ctggataagg cagatggagc aatctcgtta gaagatggaa ttattagccg aaatggtata
     6121 gccttattgg atgattctca aggatttatt tgggatgaac aatctggtta tattgagaga
     6181 gaaaatcaaa ttgacctgta tttctttgcc tatgggcatg attatagagg agcaatcaga
     6241 gatttttacc atttgactgg ttcaacaccc ttgttgccaa gatatgcttt aggcaattgg
     6301 tggagtagat attggcctta tacgtcggat gaatacttgg atttaataga cagatttgaa
     6361 acagagaaaa ttccattatc tatcggtgtg ttagatatgg attggcatat aactgacatt
     6421 ccagctcgct ttggaagtgg ctggacagga tatagttgga atagaaactt aataccaaat
     6481 ccagaacagt tattgcaaca acttcatgat agaaagctaa aactctcctt aaatgtccat
     6541 cctgctgatg ggatacgggc ttatgaagaa gcttatcctc aaatcgcaaa acggttgggg
     6601 ttaaatgtag aactagaaga acctgctatt tttgattttt ttaatccctc ttttagggaa
     6661 gcctacttta aagatgttca ttatgaacta gaaaagcagg gagtagattt ttggtggatt
     6721 gactggcaac aagggacaca aggtatgcta gatccacttt ggcttttaaa ccattatcac
     6781 tatcaggata gttgtaaaaa tgcagaaggt ggtttgattt tatcaagata tgcaggtcct
     6841 ggtagtcacc gctaccctgt tggtttttca ggggatacta ttattagttg gaattcctta
     6901 agatttcaac cctattttac agcgacagca tctaatatcg gttatagttg gtggagtcat
     6961 gatatcggtg gacatatgtt gggggattat gacgaagagc tacaaactag atggctacag
     7021 tttggtgttt ttagtccgat tactcgatta catagttcta gaagtccttt taatagtaaa
     7081 gaaccttggt ttttttcaga aacaacatct aagattatga agaaatacct tcgtttgaga
     7141 catcagatga ttccctatct atataccatg aatgtacaga cacatgagga aggtgcccca
     7201 ttaatcagtc caatctatta tttctatcca gagaatgatg agagctataa tgttccaaac
     7261 caatactttt ttggaacaga actgatggtg gctcccattg tagaaaagat ggatttgaca
     7321 ttccaatctg caaaagtaga tgtatggttc cctgaaggtg aatggtatga cttcttttca
     7381 gagaaaaaat acacaggtgg tgtgaagtta agtgtttata gggacatctc gactacgcct
     7441 gtgtttgcaa aaagtggtgc aatcattccc ttggttggtt ctgagatagg tatgggtgtt
     7501 gatttacctg aagttgtaga ttggtatgta ttcccaggaa aacaacattc ttttgaaatg
     7561 cttgaagatc aaaatggtca aagatataaa acaagattat caatcgattg ggaaatggga
     7621 atggtagagt tagcattaca aggagattct agtatcgttc caagcaatag aaaacataga
     7681 attcatttta aaggaacgaa tgtgtctata attgaattgc caaataagaa tgatacagct
     7741 aaatttgaat ggaaagataa taaaaggaca tctttaaatg acgaagtttt tagactacta
     7801 aagacagctt ctcttccata tgaattaaaa gatagattgt taaatcaatt catcaatgcg
     7861 aaaaactctc atgacttaat gaatatcttg catcatcagg ataaggaatt gagagggcgt
     7921 ttgttggaaa tgatatttac tagccaaaac taattgaaat aattagaaag tcatttgttt
     7981 agtgtatatg aagattctgt atggtattcg ttatcactgg tcacattgaa attgtgaaat
     8041 aagcacctac aacatagact ttacaacact tcatgtttca tatcatgggg tgtttttcta
     8101 tttaagtcaa agcccatcaa aaaatctcac tattcatcag gttaaaggaa acctaatcat
     8161 agtgagattg tgaattatag tagagtttgt atcgcctctt caatttgttt tgggtctgtt
     8221 tttgaagaaa agcgttcaaa gacttgccca tctcgaccga tgagaaactt agcgaaattc
     8281 cattcgactc gttttcctag tgggccggat ttctggtctt gtaaccagac atagagaggg
     8341 tctgcttcct taccgttgac cttaatcttg gcaaaacgtg ggaaggtggt ttgaaaatgt
     8401 aggctacaga aggcgttgat ttcctctgcg ctgccgggtg cttgtcccat aaactgattg
     8461 caagggaaat ccaatatttc gaagccctga tcttgatagc gttcatagag ttcttgaagt
     8521 ccctggtact ggggcgttaa accacatcca gtagcagtgt tgacaatcaa gagaactttg
     8581 ccacgatagc tatccaaggg agttgcttgg ttgttttggt tcaaaacgga aaaatcataa
     8641 agtgaagtca tgagggcctc ttttcttttt ctacattgta acagttttta cttttttcgt
     8701 cgagtcggat ggaagatatt gttgaagaat aagccttcaa tatgatagaa accgtttaca
     8761 aataacttta aaaatggtat gatttaatta aaccggttta cttagttgct ataaatctct
     8821 aatttgagat ttagttccta ttgattttac aatatgttta ttggagtgta tacatgcaaa
     8881 caaaaacaaa gaagctcatt gtgagtttgt cttcacttgt tttatcagga tttttattaa
     8941 accattatat gacaattgga gcggaagaaa cgactacgaa taccattcag caaagccaga
     9001 aggaagttca gtatcagcaa agggatacaa aaaatttagt tgaaaatggt gattttggtc
     9061 agacggagga cggaagcagt ccgtggacag gaagcaaagc tcaggggtgg tcagcttggg
     9121 tagaccagaa gaatagtgca gatgcctcaa ctcgagtcat tgaggctaag gatggggcta
     9181 tcactatctc aagccatgag aaattaaggg cagcgcttca ccgtatggtt cctattgaag
     9241 ctaagaaaaa gtataaactg cgtttcaaga ttaaaacaga taataaaatc gggattgcca
     9301 aagttcgtat cattgaggaa agtggtaagg acaagcgatt gtggaattct gcaacgacgt
     9361 caggaacaaa ggactggcag accattgaag cagactatag cccgacttta gatgttgata
     9421 aaatcaagct ggagttattc tatgaaacag gaactgggac tgtttccttt aaggatattg
     9481 agctggtaga ggtagcagac cagctttctg aggattctca aacagataaa cagcttgagg
     9541 aaaagattga tttaccaatt ggaaaaaaac atgttttttc tcttgcggac tatacttata
     9601 aggtagaaaa tcctgacgtt gcttcagtca aaaatggaat tttagaacct cttaaggaag
     9661 ggacaaccaa tgtcattgtc agtaaagatg gcaaggaagt gaaaaagatt cctttgaaga
     9721 ttctagcctc tgttaaggat gcatacacag cccgtttgga tgactggaat ggcatcatcg
     9781 ctgggaatca atactatgat tctaaaaatg aacagatggc caaattaaac caggaattgg
     9841 aaggaaaggt agctgatagc ctatctagta tttcaagtca ggcggaccgc acctatttgt
     9901 gggaaaaatt ttcaaattat aaaatgtctg caaatctgac tgccacttat cggaaattgg
     9961 aggagatggc caagcaagtg accaatcctt cttctcgtta ttatcaagat gaaactgtcg
    10021 ttcgaacagt cagggattcc atggaatggc tgcataaata tgtctacaat agtgaaaaga
    10081 gcattgttgg gaactggtgg gattatgaaa tcggtacacc tcgtgccatc aacaatacct
    10141 tgtctctgat gaaagaatac ttctctgatg aggaaattaa aaaatataca gatgtgattg
    10201 aaaaatttgt accagatccc gaacatttcc gaaagacgac tgataaccca ttcaaggctc
    10261 taggtggaaa cttagttgat atgggaaggg taaaagtaat agctggttta ctgcgtaagg
    10321 atgatcaaga aatttcttct accattcgct cgattgagca agtgttcaag ttggtagagc
    10381 aaggtgaagg tttttatcaa gatggatcct atatcgacca caccaatgtt gcctatacgg
    10441 gtgcttatgg gaatgttttg attgatggcc tgtctcaact gttgccagtc attcaaaaga
    10501 ccaagagtcc aatcgataaa gataaaatgc aaaccatgta ccactggatt gataaatcgt
    10561 ttgctccttt gctggtgaat ggagagttga tggatatgag tcgtggacgc tcgatcagtc
    10621 gtgcaaatag cgaggggcac gtggccgcag tagaagtact aagagggatt caccgaatag
    10681 cggatatgtc tgaaggagaa accaaacaac gtttgcagag tcttgtgaag accattgttc
    10741 aatcggatag ttattatgat gtctttaaga atttgaagac ttataaggat atcagtttga
    10801 tgcaatcctt gttaagtgat gcaggagtcg caagtgttcc aagaacaagt tacctatctg
    10861 cctttaacaa gatggataaa acagccatgt acaacgcaga gaaagggttt ggatttggct
    10921 tgtcactctt ttccagtcgt accttgaatt acgaacacat gaacaaggaa aataaacatg
    10981 gttggtatac gagtgatggg atgttctatc tttacaatgg cgatttgagt cactatagcg
    11041 atggctactg gccaacagtt aatccatata agatgcctgg tacaacagag acggatgcta
    11101 agagagcgga tagcgataca ggtaaagttt taccgtctgc tttcgttgga acgagcaaac
    11161 tagatgatgc caatgcgaca gcaaccatgg atttcaccaa ctggaatcaa acattgactg
    11221 ctcataagag ctggtttatg ctaaaggata agatcgcctt tttaggaagc aatatccaaa
    11281 acacttcaac agatactgct gcaactacaa ttgaccagag aaaactggaa tcaagtaatc
    11341 catataaagt ctatgtcaat gataaagaag cctcccttac agaacaagaa aaggattatc
    11401 ctgaaaccca aagtgtcttt ttagaatcgt ccgattcgaa aaagaatatt ggttactttt
    11461 tctttaagaa gagttcaatc agtatgagta aggctttgca aaagggagcc tggaaggata
    11521 tcaatgaagg acagtcagac aaggaagttg aaaatgaatt tcttacgatt agtcaggctc
    11581 ataagcaaaa tggagattct tatggctata tgcttatccc taacgtggat cgtgccacct
    11641 tcaatcaaat gataaaagag ttagaaagta gcctcatcga aaataacgaa acccttcagt
    11701 ctgtttatga tgctaaacaa ggagtttggg gcattgtgaa atatgatgat tctgtctcta
    11761 ctatttccaa ccaattccaa gttttgaaac gtggagtcta tactattcga aaagaggggg
    11821 atgaatataa gattgcctac tataatcctg aaacccagga atcagctcca gatcaggaag
    11881 tctttaaaaa gctagagcaa gcagctcagc cacaagtaca gaattcaaaa gaaaaggaaa
    11941 aatctgaaga ggaaaagaac cattcggatc aaaagaatct ccctcagaca ggagaaggtc
    12001 agtcaatctt ggcaagtcta gggttcttgc tacttggggc gttttatcta ttccgtagag
    12061 gaaagaacaa ctaatttgtt catcatctag atgaattaca tgaaactgtg gaaaatagaa
    12121 aaaatccgaa gaaggccaaa tatcttcgga tttttctttt aaaaaagata agttgatact
    12181 tatccttttt tacttcatta tgtgactata gcgttttgaa actagaatag gacgtcataa
    12241 ctgctaaaag atttctataa attcatttga ttttcctaat caatttgttc gtatcctatt
    12301 tcactccact atattagtcc gtctcgctcc gtgaggtgcg agacaaaaca accacccgct
    12361 atacgggtgc gcgtcgaagg ttataccaaa aaaactccaa acgcgataca ataaaggtgt
    12421 tcaatccaat tgtaaagcga aaggagaaaa aatatggcac aaaagactca tagtttatca
    12481 tacacaaagt ggatgtgttc tatcatattg tgttcacccc taagtataga cgaaaagtca
    12541 tctataatca atatagaagt agtttaggcg aaatatttca tcgcttgtgt agttataaag
    12601 gtgttgaaat tatcgagggt cacttaatgc cagaccatgt acacatgtta gtcagtattc
    12661 cactaaggct aagtgtttca agttttatgg ggtatttaaa aggcaaaagt gcactcatga
    12721 tgtttgacaa acacgccaat ctcaagtaca agtttgggaa tcggcatttc tgggcggaag
    12781 gttattatgt aagtacagta gggcttaata aagccacact taagaaatat agtcaagatt
    12841 aaagaaatta tccagtggat gatttcttca cgagtatgaa aatttgagaa ctagtaaagt
    12901 atgatatata gtaaaatgaa ataagaacag gacaaaatcg atcaggacag tcaaatcgat
    12961 ttttaacaat gttttagaag tagaggtgta ctattccagt ttcaatctac tatagcacta
    13021 gataaattga gtgtaaaaga atatgaggat ccctttaggg atagtggtaa gtaataccaa
    13081 agtctcttaa agaggcaagt gacgagtcaa gagcaataag gcttgaacaa cgtgaaagcc
    13141 agcgtcttta ggcgctggct gatgatttgg gcttatagct ctgagataaa ccacccgtta
    13201 gacgggtagt tatgatttta tctgagtgta acatactgtt gggcaatctc gctgatgcgg
    13261 tcaaagttgc cttgggaagc gagtttattg agttcgccac caattccaac ggcatctgca
    13321 ccagcagcga accattgagg gatgttgttt aggccgactc ctccggttac cattacggaa
    13381 acttgtggga tcggtgcctt gactgcagag atatatgctg gactgagagt actacctggg
    13441 aagagtttga tgatttcact accggcttca agtgcagtcg tgatctctgt gagggtaata
    13501 cagcctggaa tgtacggtgt gctgtagaga ttgcacattt tcgcagtttc agcatggaaa
    13561 gatggagaaa caacgtaatt tgctccagct agaatggcat ctctagcagt tacggcatca
    13621 agcacagtac ctgcaccgat acaaacactc tgatcgtcct gatacaagtc tacaagttcc
    13681 ttgatgattt gtcctgcata ctgattggta taggcgattt caatagcttt gataccgccc
    13741 ttgatacaag caatcgaggc ttgtagtcct tcttcctttg tatttcctcg aatgacagcg
    13801 acaattttct gtttttttag ttcaataatc gtatctgatt tggtcatgta attctcctaa
    13861 cgaatgatat cttgtgcatt tgccagtaaa ttttcaatac tagttgcgga agtggagaga
    13921 tggtctcctg gaagagtgca tttgagagtt gcgctcgcaa ctgcaaagtc aatggtagtt
    13981 tttagggagg aatgatggag tagttggtaa agggcaccag atataaaggc atccccgcta
    14041 ccaattcgtt gatagactgc agtttttagt tggacagact cttcaaatcg ttcttccaga
    14101 gcataggctt gatagacatt tttgtcttgc tcatcactag atcggagggt atggaaaatg
    14161 gccttgaaac cataggcttc ttttaaaagt cgcatgcgat tttccacctc ttctaggcta
    14221 gcactgtctc ttggaaacat ctctagattt tggtcatcaa tcatgagagg atcaataccg
    14281 aagcaatagt cagtaaaacg tgcaaactta gaaaattcat acttggcttc taggactgaa
    14341 atcatctttg ttctcagatt gagatccatt gaaacgacaa ttcctcggcg cttggcttct
    14401 tccaagagta ggagaaggat cgcacggacc tcttgaccga tagctatggt gattccacta
    14461 aaatgaaaat ggctaatccc ctgaaagaga gaatccatat ctagcatgtt tggccgaatc
    14521 tggctgatac tcgtatgctt acgatcgtag aaaacttcac tttgacgaca accaaagccg
    14581 ttctccaaat agtagaggcc gattcgatcg ccaagccgac agattgaact ggtatcgatt
    14641 tggtgctgtt tcaagaatgt gagaaaacga tctccaatct cgttggcagg gagtgcggta
    14701 aagactttcg ttgagatacc cagggcttgc aaattacaag cgatgttaat ttctgatccg
    14761 ccaaaataag tcgaactggc aacatgatcg ccgatactgg tggcatctaa tggtgagatt
    14821 cgaattaatg gttcgccaaa cagtaatatt ttcgtcatcc attagttcct ttctggttta
    14881 gaatagcttt acttggctaa tacttctttc aaataagcag cgacttcatc atcttggcag
    14941 ttttcaaaga agtattcttg gaagtatttg ccagagatgg tgtctttgag gaagtcttgg
    15001 tcgattgttt taaggatggt catcaaatca ttgtgggtga tttgtttcac ctcgtttaag
    15061 atacgagcgt tgcgttgttc agggattaca cgttctcttg ggtagccacc gcccatttct
    15121 tcagcaaata agcgttcaaa catcaatttg agggtcagtt ctgcccccca gccaaatcct
    15181 ttggcataag ggatagacaa ggcgttacca ccattgattt gagaataaag gtaagcgtca
    15241 gttgggtcca ctgctagacc acagacaaca ccagggaagc tgtttaaagc aagcatagcc
    15301 cctacacccg taccacagcc ggtaacaaca aagtcaactg cctttgtatt taaaaggatg
    15361 gcagccatta gtccgttctg cacataagtt aattgacttt ctccttcttc tccacgcata
    15421 ccatagttaa ataattggta gtctttttta tctgtcgctt cttttagact atcgtaaata
    15481 atgtgattct tgctagcttg actattttca ttgattaatg cgattttcat aagatcttct
    15541 ttctattttt actcaggttg ttttccgatg taggctaaga taccgccatc tacatatagg
    15601 atgtggccat tgacaaaatt gctggcatca ctagcgagaa agacagcagg gcccatcaaa
    15661 tcttcaggat ttccccaacg tgcagcaggt gtttttgcaa tgatgaactg gtcaaatggg
    15721 tggcgagaac catcttcttg caactcacga agaggtgctg tttgaggagt ggcaatataa
    15781 cccggtccaa ttccgttaca ttggatattg gctccaccgt attcagacgc aatgttgcgg
    15841 gtcaacattt tcaagccccc tttagcagca gcataagcgc taactgtttc acgtcccagt
    15901 tcgctcatca tcgaacaaat attgataatc tttccatgcc ctttctttat catagaagga
    15961 ataactgcct ttgaaacgat aaatggtgcg tttaagtcaa tatcgatgac cttacggaaa
    16021 tcagcggcgc tcatttcgca cattggaact cggcggataa taccagcgtt attaacgagg
    16081 atgtcaatga caccaacctc ttgttcgatt tgcttgacca tggcttggat accgtcctcg
    16141 tctgtcacgt cacagacata tccatgggct tcgatgccaa cttcacgata agcttcaatc
    16201 cctttattga ccagatcttg attgatatcg ttaaagacaa tagtagcgcc ggcttcagcg
    16261 taggatttgg caatagcaaa tccaattcca taagaagcgc cggtgatgag agcaattttt
    16321 ccttttaaag aaaactgctc aattgagaat gatgtatttg tcatttgata cttcctttca
    16381 aatttgttaa gacttctcta taa
//
LOCUS       DBERDK010000043        14463 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.043, whole genome shotgun sequence.
ACCESSION   DBERDK010000043 DBERDK010000000
VERSION     DBERDK010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 14463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14463
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.043"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(44..1180)
                     /gene="dnaJ"
                     /locus_tag="ACL73L_001380"
     CDS             complement(44..1180)
                     /gene="dnaJ"
                     /locus_tag="ACL73L_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HGS0772342.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKEPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGANGGFGGDGGFGGFNGAGGFGGFEDIFSSF
                     FGGGGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPGT
                     SPVTCGRCHGAGVINVDTQTPLGMMRRQVTCDVCHGQGKEIKYPCTTCHGTGHEKQAH
                     SVHVKIPAGVETGQQIRLAGQGEAGFNGGPYGDLYVVVSVEASDKFEREGTTIFYNLN
                     LNFVQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRSKGAPSLRGGAVGDQYVTVN
                     VVTPTGLNDRQKVALKEFAAAGDLKVNPKKKGFFDHIKDAFDGE"
     gene            complement(1582..1938)
                     /locus_tag="ACL73L_001381"
     CDS             complement(1582..1938)
                     /locus_tag="ACL73L_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine-N7)-methyltransferase"
                     /protein_id="HGS0772343.1"
                     /translation="MSVLDDEYLKNTRKVYNDFCNQADNYEAAKGFIDNIPVECLTIY
                     REIILAEHDSCVKNDEAVRNFVTSVLLSAFVSAMVSAMISLEIQTYKFVIPFIIGMIW
                     TVVVFLMINWNYIGKY"
     gene            complement(1940..3763)
                     /gene="dnaK"
                     /locus_tag="ACL73L_001382"
     CDS             complement(1940..3763)
                     /gene="dnaK"
                     /locus_tag="ACL73L_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HGS0772344.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLTRAKFD
                     DLTRDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRNE
                     VDQAIFATEKTIKETEGKGFDAERDAAQAALDDLKKAQEDNNLDDMKAKLEALNEKAQ
                     GLAVKLYEQAAAAQQAQEGAEGAQATGNAGDDVVDGEFTEK"
     gene            complement(4243..4767)
                     /gene="grpE"
                     /locus_tag="ACL73L_001383"
     CDS             complement(4243..4767)
                     /gene="grpE"
                     /locus_tag="ACL73L_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HGS0772345.1"
                     /translation="MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFEN
                     KYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKG
                     LAMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPADDEHPVDTIAQVFQKGYKL
                     HDRILRPAMVVVYN"
     gene            complement(4794..5828)
                     /gene="hrcA"
                     /locus_tag="ACL73L_001384"
     CDS             complement(4794..5828)
                     /gene="hrcA"
                     /locus_tag="ACL73L_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HGS0772346.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKTLQESINSSSATIRNDM
                     AELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFDRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTDLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKRIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVMAGKVNLLNFANLAAYQFFDQPQKVALEIREGLREDQMQNVRVADG
                     QESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQVNVVNRVLTMKLTDFYR
                     YLSSNHYEVH"
     gene            complement(5992..6501)
                     /locus_tag="ACL73L_001385"
     CDS             complement(5992..6501)
                     /locus_tag="ACL73L_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HGS0772347.1"
                     /translation="MNSRVEFRIFTIVDLDKKEEYLHEMHLKGWRYRTSRFGLFYFEQ
                     GQPDDVIYRIYDSRFLKEHKRELQDFRDRGWELIGAGSCSILRKSSSDLLPEDQVYMS
                     KGLKWEVMRSRLRSCTATFLGGLVVCMSLFREDLSMSFFLIFVLYAFLISYLIHGYFR
                     LKRKYRVDE"
     gene            7110..9443
                     /gene="hsdR"
                     /locus_tag="ACL73L_001386"
     CDS             7110..9443
                     /gene="hsdR"
                     /locus_tag="ACL73L_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EcoAI/FtnUII family type I restriction enzme
                     subunit R"
                     /protein_id="HGS0772348.1"
                     /translation="MVEFIKSKKEMSEEDIKANFITPAIVSKGWKNGEHIAYEEYFTD
                     GRIEVRGDKARRKEGKKSDYSLYYQFGTRIAIVEAKDNKHSVRAGLQQAIEYGEILDV
                     PFVYSSNGDGFIEHDRITREERELELDEFPTREELFSRMTKEKGLTYEITEAISTPYY
                     TDAFSMKTPRYYQQIAINRTIETVARGQKRVMFVMATGTGKTFMAFQIIHRLRKAGLA
                     KRVLFLADRNILVDQTMAEDFKPFEKVMTKITPKLLTAPEKLNSFEIYLGLYQQLTGE
                     DGTETHYQKFDKDFFDLIVIDEAHRGSAKEDSNWRKIIDYFSSATQIGMTATPKETKN
                     ASNTEYFGEPIYTYSLKQGIEDGFLAPYRVMRVNLDVDVDGYRPETGKVDANGQLIED
                     RYYGRKDFDKTIVIDDRTQRVAKFVSDYMKQNNARFDKTIVFCVDIDHAERMRAALVK
                     ENLDLVQEDYRYVMQVTGDNAEGKAQLDNFMDVNSNFPAIVTTSKLLTTGVNAKTCRL
                     IVLDSNIQSMTEFKQIIGRGTRLYPQKGKEFFTIIDFRNVTNLFADPDFDGDPVKVLE
                     TGAKTVSGSTPGFVDEEGDPVEKYIVTDKQVTILNSTVQVLDENGKLITESLTDYTRK
                     NILGSYATLNDFITVWHTADKKKLILDELYKKGVYLDAIRESEGISEQEIDDFDLLLK
                     LAYGQKELTKTERINKLKQSGYLYKYSEEARAVLEILLNKYMDKGIGELESIETLKLP
                     EFQIYGGTFKIINTYFGDKKRYLQAIKELEQELFTVA"
     gene            9456..10919
                     /locus_tag="ACL73L_001387"
     CDS             9456..10919
                     /locus_tag="ACL73L_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HGS0772349.1"
                     /translation="MSITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREM
                     VWELEEDEYESIIPEELKWRNWAHAQNGERVLTGDELLDFVNNKLFKELKELEITSNM
                     PIRKTIVKSAFEDANNYMKNGVLLRQVINVIDEVDFNSPEDRHSFNDIYEKILKDIQN
                     AGNSGEFYTPRAATDFIAEVLDPKLGESMADLACGTGGFLTSTLNRLSSQRKTSEDTK
                     KYNTAVFGIEKKAFPHLLAVTNLFLHEIDDPKIVHGNTLEKNVREYTDDEKFDIIMMN
                     PPFGGSELETIKNNFPAELRSSETADLFMAVIMYRLKENGRVGVILPDGFLFGEGVKT
                     RLKQKLVDEFNLHTIIRLPHSVFAPYTGIHTNILFFDKTKKTEETWFYRLDMPDGYKN
                     FSKTKPMKSEHFNPVRDWWENREEILEGKFYKSKSFTPSELAELNYNLDQCGFPKEEE
                     EILNPFELIQNYQAERATLNHKIDNVLADILQLLEDK"
     gene            10919..12487
                     /locus_tag="ACL73L_001388"
     CDS             10919..12487
                     /locus_tag="ACL73L_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGS0772350.1"
                     /translation="MTPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLI
                     SEGKIKRDKKETEIFRGDDGKHYGKFADGSTQEIDVPYDIPDTWEWVRFSTLVEIVRG
                     GSPRPIKDYLTSEVDGINWIKIGDTEKGEKYINNVKEKIKKSGLNKTRFVKKGTFLLT
                     NSMSFGRPYILNVDGAIHDGWLAISNYENSLNKDYLFYILSSNVVYSQFLSLISGAVV
                     KNLNSDKVASILIPLPPLSEQQRIIEAIESALEKVDEYAESYNRLEQLDKEFPDKLKK
                     SILQYAMQGKLVEQDPNDESVEVLLEKIRAEKQKLFEEGKIKKKDLDISIVSQGDDNS
                     YYEEVPCEIPESWEWVRLNDITSYIQRGKSPKYSNIPIYPVIAQKCNQWSGFSIDLAR
                     FIDPETVHSYQKERLLRDGDLMWNSTGLGTLGRLAIYHENKNPYVWAVADSHVTVIRV
                     LSGVINCHFIYNFLSSPIVQSVIEEKASGSTKQKELLTKTIKEYLIPLPPLPEQSRIV
                     DKIEQFFAHIDALI"
     gene            12544..13341
                     /gene="psrA"
                     /locus_tag="ACL73L_001389"
     CDS             12544..13341
                     /gene="psrA"
                     /locus_tag="ACL73L_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase PsrA"
                     /protein_id="HGS0772351.1"
                     /translation="MKDFELHLKKAGLAENTVRSYLYGVRFFLENYELKMEDLFEYKG
                     YLLDNFKPKTVNLRLQGVNKYLAFIGHDDLKLKFVKVQQKPFLEDVISHADYLFLKRS
                     LKKDGILKWHFVVWFLGATGARVSELIKLKVEHVEIGYFDIYSKGGKIRRLYIPKKLR
                     NSCLSWLESENRRSGYLFLNKFNEPITARGVAQQLKNYADKYKMNPKVIYPHSFRHLF
                     AKNFLAKYNDIALLADLMGHESIETTRIYLRKTATEQQNIVDKIVNW"
     gene            13352..13960
                     /locus_tag="ACL73L_001390"
     CDS             13352..13960
                     /locus_tag="ACL73L_001390"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001859489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGS0772352.1"
                     /translation="MVKLTTCYFCDYGSYYGNKDETTSYPIYEIPEAWRYIKFASLVN
                     FRIGKTPPRSEATFWGTEIPWVSISDMPISGYVTNTRESISKLALKSKKIDISPKGTL
                     LMSFKLSIGKVAILDIPATHNEAIISIFPYANKENIIRDYLMIFLPLISTLGDSKDAI
                     KGKTLNSTSISELLIPISNHEEMKRIIFKVDLLFQKVSQLFE"
     gene            complement(13914..>14463)
                     /locus_tag="ACL73L_001391"
     CDS             complement(13914..>14463)
                     /locus_tag="ACL73L_001391"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGS0772353.1"
                     /translation="NIPMNWVVIKIKDIFSMNTGLSYKKGDLSINNKGVRIIRGGNIK
                     PLEFSLLDNDYYIDTQFISSEQVYLKHNQLITPVATSLEHIGKFARIDKDYDGVVAGG
                     FIFQLTPFESSEIISKFLLFNLSSPLFYKQLKAITKLSGQALYNIPKTTLSELLIPLA
                     PFEEQELITQKVEKLFEKVNQL"
BASE COUNT         4578 a         2682 c         2726 g         4477 t
ORIGIN      
        1 gcaagccgac gctgacgtgg tttgaagaga ttttcaaaga gtattattct ccatcaaagg
       61 catctttaat atgatcaaag aagccttttt tctttggatt tactttcaag tcaccagcag
      121 ccgcgaattc tttcaaggct actttttggc ggtcgttcaa gcctgtcggt gttacgacat
      181 taacagtaac gtattggtca ccaactgcac cgccacgaag gctcggtgcc cccttactac
      241 gtaggcggaa tttcttacca gtctgagttc cctctggaat aaccaattca acatcaccgt
      301 gaacagttgg aatatctact gtatcaccaa gagccgcttg gacaaagttg aggttgagat
      361 tgtagaagat agtcgttcct tcacgttcaa acttgtcgct agcttccaca gaaactacta
      421 catacaagtc accataaggt ccaccgttaa agcctgcttc accttgacca gcgaggcgaa
      481 tttgttgacc tgtttccaca ccagcaggga ttttcacatg tacgctatga gcttgtttct
      541 catgacctgt tccatgacag gttgtacatg gatatttgat ttcttttcct tggccgtgac
      601 agacatcaca ggttacttgg cgacgcatca taccaagagg agtctgcgta tcgacgttaa
      661 tgacaccagc gccatgacag cgtccacaag tgactggact tgtccctggc ttagcaccag
      721 agccattaca tgtacgacag ccagcttcac gatgatactt aacttccttc tcagttccga
      781 agatagcttc ttcaaaggtc aaattgacac gatactggag atcatctcct tggcgaggag
      841 cgtttggatt gcgcgaagaa ccgcctccgc cgaagaaact tgagaaaata tcctcaaaac
      901 caccgaagcc acctgcccca ttgaaaccgc cgaaaccacc atctccacca aaaccaccat
      961 tggcgcctgc agcaccatac tggtcatagg cagcacgttt ttggtcgtca ctcaaagtct
     1021 cataggcttc ttgaacttcc ttgtacttgt cctcagcacc aggctccttg ttgatatctg
     1081 ggtgatattt tttggaaagc ttacgataag cctttttgat ttcgtctgcc gaagcgtttt
     1141 tggatacccc cagacgatca taaaattcag tattgttcat aattcaagat acaaagggcg
     1201 tcaaacggac acagccatct aggagttttg acgacggacg cttatgtcct agaaaaaact
     1261 atctttttgg cacagtccgt agcccgtgtt cagttacgaa cacccttatt cctttctatt
     1321 gatgttctga atcgaaccac catttaggtc ggattcaatt cctttctttt atatttagta
     1381 agaaattctg aaacccgtgt tcagttacga acactctttg ttcctttcta ttgatgagac
     1441 aaaacccaaa caaatgagat tccgtccctt tttatcgaaa aacagaggct gggttgcaac
     1501 ctctgcaaat aagtaaatta actttatttt gattttaaat atcctataag tacagaaata
     1561 tttatatatt tttgtctctt cttagtattt gcctatataa ttccaattga tcataagaaa
     1621 tacaactact gtccaaatca taccaattat gaacggtatt acaaatttat atgtttgtat
     1681 ttctaatgat atcatagctg ataccatcgc cgatacaaat gcagacaaca atactgaagt
     1741 aacaaaattc cttaccgctt catcattttt gacacaacta tcatgctcag ctaatataat
     1801 ttctctatat atagtcaaac attctactgg aatattatct ataaaacctt ttgctgcttc
     1861 atagttgtca gcttgattac aaaaatcatt ataaactttt cgtgtatttt ttagatactc
     1921 atcatccaat acactcatct tatttttccg taaactctcc gtctacgacg tcatcgcctg
     1981 cgtttcctgt tgcttgtgcg ccttctgctc cttcttgagc ttgttgcgct gctgcggctt
     2041 gttcgtagag tttcacagca aggccttgag ctttttcgtt caacgcttca agtttcgctt
     2101 tcatgtcgtc caagttgttg tcttcttgag ctttcttaag gtcatcaagg gcagcttggg
     2161 cagcgtcacg ttctgcgtcg aagcctttac cttcagtttc cttgattgtc ttttcagtcg
     2221 caaagattgc ttggtctact tcattacgaa ggtctacttc ttctttacgt ttcttatcag
     2281 cttcagcgtt tgcttctgca tctttcatca tgcggtcgat ttcttcgtca gtcaaacctg
     2341 agttcgattg gatgacaata gtttgttctt tttgagttcc aaggtctttg gccttaacag
     2401 acacgatacc gttcttgtcg atgtcaaatg ttacttcgat ttgaggaatt ccacgaggtg
     2461 cagctgggat atcagtcaat tggaagcgtc caagagtctt gttatctgct gccattgggc
     2521 gttcaccttg aagaacgtgg atatcaacgg ctggttggtt gtctgctgct gttgagaaga
     2581 cttgtgattt agatgttggg attgtagtgt tgcgatcgat aagttttgta aatactccac
     2641 ccattgtttc gataccaagt gacaatggcg ttacatcaag aaggacaacg tccttgacat
     2701 caccagtaat cacaccacct tggatagccg cacccatagc aactacttca tcagggttta
     2761 ctgatttgtt tggttcttta ccagtttcag ctttaacagc ttcaacaacg gcagggatac
     2821 gagttgaacc accaacaagg ataacttcgt cgatttctga caagctcaaa cctgcatctg
     2881 aaagggcttg acgaactgga acttttgtac gttcaacaag gtcacgagtc aaatcgtcaa
     2941 atttcgcacg agtcaaagtc atttccaagt gaagaggtcc agcctcacct gcagtgataa
     3001 atggcaagct gatttgtgtt gaagttacac cagaaaggtc tttcttcgct ttttcagccg
     3061 catctttcaa acgttgcatt gccatcttgt cagtagacaa gtcgataccg ttttctttct
     3121 tgaattctgc taccaagtgg tcaatgattt tttggtcaaa gtcgtcacca ccaagtttgt
     3181 tgtcccctgc agttgacaat acgtcgaaga caccgtcacc caattcaagg atagagacgt
     3241 cgaatgtacc accaccaagg tcaaatacca agattttttc ttctttgtca gtcttgtcca
     3301 aaccataagc aagagctgct gcagttggtt cgttaacaat acgttctact tcaagaccag
     3361 caattttacc agcgtctttt gttgcttgac gttgagcgtc gttgaagtaa gccggaactg
     3421 tgataacagc tttggttact ttctcaccaa ggtagtcttc agcgtagcct ttcaagtatt
     3481 gaaggatcat agctgagatt tcttgtggag tgtattcttt tccatttgca gaaacttttt
     3541 cagaagttcc catcttagat ttgatagaga taactgtatc tgggtttgta actgcttgac
     3601 gttttgcagc atcaccaacg atgatttctc cgtttttgaa tgagactaca gatggagttg
     3661 tgcggtttcc ttctgggttt gcgatgattt tgctttcagt tccttcaaga actgcaactg
     3721 ctgagtttgt tgtacctaag tcaataccga taattttaga catgtgtttt tctccttgtt
     3781 agttaatttt ctatttttct ttttgatact cttcttaagt ggcgacgccg tcagagcttg
     3841 acttcgtcaa tctctttgac taaactttga gcctaaggtc tcaaaatttg cgcaaatagc
     3901 accacggcga agagtatcgt ctttggttcg cttcgctcac tattacaaag ccaaacatat
     3961 tttatctttc ttcatagttt attgtcgttt cggacaagtt ttctattatg taaattgcga
     4021 cacgaggagt cgaaatcgat tttatttcga cgacgagtta gtaaggaagc taggcaaacg
     4081 ccatagcgat tgccgttttc tgacgagttg ctttagctac cgtcagaatt gccgaatcga
     4141 acacgcccta agctctgagt aaaaaagata gattttcttg tgttcatcga acacttcgtc
     4201 aatctcctat ttttactgcg agctttttac gagctttgta tcttagttat acaccactac
     4261 cattgctggg cgtaggatgc ggtcatggag tttgtagcct ttttgaaaga cttgggcgat
     4321 ggtatctact gggtgttcat cgtctgctgg gagagtttgg atggccatat ggtagttatg
     4381 gtcaaattcg ccatctgctg cgatttcttc aattccttct tctttcaaag cgtgaatcaa
     4441 gctttcttgc accatcgcca agcccttctt cacatcatct gtcaaacctt caactgcaag
     4501 tgcacgctca aggttgtcaa gagatggtaa gattgctttt gccaagtcct ggctacgata
     4561 acgttgcaag ttttgacgtt cttcattggc acggcgttgg atattttgca tttctgcatg
     4621 agcgcgaaga tatttgtttt cgaactcatc tgcacgttca tttgccaagt ccaactcaga
     4681 cttttcagga gttgtttctt cagctgtttc cacaacttcc tcttcttgaa cttcttctac
     4741 ttcttcattt tttatatctt gggccatgtt cgcctccttt aatgatttca atcttaatgt
     4801 acttcgtaat gattactgct gaggtagcgg taaaaatctg tcaacttcat ggtcaaaaca
     4861 cggttgacca cattgacttg attgattagc tgttggtaat ccagattaac tggaccgata
     4921 atggctagaa ttccaactcc ccgataagga atgaggaact tactactaat caccgctagg
     4981 tcagctaaac aggactcttg accgtctgca acacgaacat tttgcatctg atcctcacgc
     5041 aacccctcac gaatctccaa ggccaccttt tgcggttggt caaagaactg ataggctgct
     5101 agattggcaa aattcaagag attgaccttg cccgccatca caatgttttc gttgaacatt
     5161 tccttaaaga tgtgttcaaa gagatcgatg acattatccg ttgttgtaaa gtaacgctgg
     5221 ataatctgcg gaatctccgt ccgaatcttg tagtgaatat ctaaaacggt gtgaccgagg
     5281 aaacgttcct gaatgattct cttcagtttc agcaaatcct cctgcaagaa gttccttgga
     5341 atcagaaact gactagtaac cgttcgcgac tcgtctaggg taaataccgc caaggctgta
     5401 tgttgcccca aaacaacgat atcaaaggct gtcaaccgtt gcctgctcgg ctcaacatcc
     5461 agtgccacta ccgtacagcc actcaggtct gttagcaagt tagcagcctc ttgcagaata
     5521 tcctccaatt tgaagaattc ctgatcaaag gctttgacaa tctcatatac ctcattttca
     5581 gccagtcggt caaaatccag tgagtgtttc acatagtact gaaaaccagc aacacttggc
     5641 atccgaccgc ttgaagtatg agccttctca agcaaccctt gtttttctag ttccgccatg
     5701 tcattacgaa tggttgcact gctagagtta atagactctt gcaaggtttt tgatccgaca
     5761 ggttcgtgcg ttttggtaaa gatgtcaata atcagattta aaatatcctg ctgacgctct
     5821 gtaaccatct catcactcct ctctgtctga tcaattagca ctcaatgcat ccaagtgcta
     5881 actcatgatt ctattataca cccttagaag agaatgtcaa gacaaaaact caaaaaatta
     5941 gcactctttt atcaagagtg ctaaaaatca atctgaagac ctagaacctt gctattcatc
     6001 tactcggtat ttccttttta gtctgaaata accatgaatt agataagaaa tcagaaaagc
     6061 atataaaaca aaaataagaa agaaagacat agaaagatcc tctctaaaca aactcataca
     6121 aacaacaaga ccacctaaga aagtagctgt acaggaacga agtctagatc gcataacttc
     6181 ccatttgagc cccttactca tatagacttg atcctctgga agtaaatcag aagacgattt
     6241 acgaagaatc gaacaagaac ctgctcctat caattcccaa cctctatctc taaaatcttg
     6301 cagttcacgc ttatgctctt taagaaatct agaatcatag atacgataga taacatcgtc
     6361 cggttgacct tgctcaaaat agaacaaacc aaaacgactc gttctatacc tccaaccttt
     6421 caaatgcatc tcatgtaaat attcttcttt cttgtccaaa tcaacaatgg tgaaaatccg
     6481 aaattctact ctgctattca ttgtcttacc ccaaaattag aaaacatgcc tggcgttatt
     6541 tagttagata attctttcca cttttgactc aatctccaaa aaatataaga aatctgaatc
     6601 gcaaaaacta tcaataaaac ccaatctatt atgaaaatca aaaacacttt ccaactgaaa
     6661 gaactacctc cagtaacaaa ctttgagaaa aacggtagta gagctaaaaa gagaagtaaa
     6721 ataggaagca tccgcattgt taaaatccgt ttggcataaa aaaatcttta tttaaacgaa
     6781 aatattatag caaaatttac gccagttttt gaacggctga tgtagatatt ttatactttc
     6841 aaaatgtttc aatgtgatta tttatttttg aaaaatagct caccagcccg actgaaagtg
     6901 cttatagaat gataataagt cgcctgccga aaacagcgga aaatagcggt gttatgcgga
     6961 gataatctga cgcgatgcga aagtatattg catacttatt ttcaacaatt tagcagagta
     7021 tttttataag tgtgatataa tagaagtata atttgttctg atagtttatt ttatggagaa
     7081 gtagattttt agaatgcgga gggttcaata tggttgagtt tataaagtct aagaaagaaa
     7141 tgagtgagga ggatattaaa gcaaatttca tcactcctgc tattgtatcc aaaggatgga
     7201 aaaatggtga gcatatcgct tacgaagaat acttcactga tggtcgaatt gaggttagag
     7261 gagataaggc tcgtcgtaaa gaaggaaaaa aatcagacta ttcactgtat taccaatttg
     7321 gaactcgaat tgcaattgtt gaggcaaagg ataataaaca cagcgttcga gcaggattac
     7381 aacaagctat tgaatatgga gagattttag atgttccatt tgtttattct tcgaatggtg
     7441 atggctttat tgaacacgac cgtatcacga gagaagaacg tgagctggag ttagacgaat
     7501 tccctactcg tgaagaatta ttttctcgta tgacgaagga aaaaggattg acgtacgaaa
     7561 ttacagaagc tatctcaact ccatactata cagacgcctt ctcaatgaaa acgccacgct
     7621 attatcagca aatagctatc aaccgtacta ttgaaacagt tgccagagga caaaaacgag
     7681 taatgtttgt gatggcaaca ggaacgggga aaacgttcat ggcttttcaa attattcatc
     7741 gccttcgaaa agctggtttg gctaaacgag ttttattctt agcagataga aacatcttag
     7801 tagaccaaac gatggctgaa gactttaagc cattcgaaaa ggtaatgacg aaaattacac
     7861 caaaactttt gactgctcct gaaaagttaa attcttttga aatttatcta gggctttatc
     7921 agcaactaac tggtgaagat ggaactgaaa cacattatca aaaatttgac aaagacttct
     7981 ttgatttaat cgtaattgat gaagcgcacc gtggttcagc taaggaagac agtaactggc
     8041 gtaagataat tgattatttc agttctgcga cacagattgg gatgaccgct actcctaaag
     8101 aaaccaagaa tgcttccaat acggaatact ttggtgagcc aatctatact tatagtttaa
     8161 aacagggaat cgaggatggt tttttggctc catatcgtgt tatgagggtt aatttagatg
     8221 tggatgtgga tggttatcgt ccagaaactg gaaaagttga tgctaacgga caattaatag
     8281 aagataggta ctacggcagg aaagattttg ataaaaccat tgtcattgat gatagaacgc
     8341 aaagagttgc caagtttgtt tctgattata tgaagcaaaa caatgcacga tttgataaaa
     8401 caattgtttt ttgtgttgat attgaccatg ccgagcgaat gcgtgctgca cttgtaaaag
     8461 agaatctaga cttagtccaa gaagactatc gttatgtcat gcaagtaact ggtgacaacg
     8521 ctgaaggaaa agctcaactg gataacttta tggatgtcaa ttctaatttt cccgctattg
     8581 taacaacgtc taaattatta acgacaggag ttaatgctaa aacatgtcgt ttgattgttt
     8641 tagactctaa tatccaatcc atgactgaat ttaaacaaat tattggtcgt ggcacacgtc
     8701 tttatcctca aaaggggaaa gaatttttta cgattattga ttttcgaaat gttaccaatt
     8761 tgtttgctga ccctgatttt gatggtgatc cagtgaaggt gctagaaaca ggtgcgaaaa
     8821 cagtcagtgg ttctacgccc ggtttcgtag atgaggaagg tgacccagta gaaaaatata
     8881 tcgttacaga caagcaggtt accattctta attctactgt tcaagtattg gatgaaaacg
     8941 ggaaactgat taccgaaagc ctgaccgact acactcgaaa gaatatctta ggtagctacg
     9001 ccactttgaa cgattttatc acagtttggc atacggcaga taagaagaag cttatcttag
     9061 acgaacttta taaaaaagga gtttatctag atgctattcg agagtcggag ggaatatcag
     9121 aacaagaaat cgatgatttt gatttactcc taaaacttgc ctatggtcaa aaagaattaa
     9181 ccaaaacgga acgtatcaat aaactcaaac aaagcggata tttatataaa tatagtgagg
     9241 aagcgcgtgc tgttttggaa attttactga acaaatacat ggataaaggt attggagaac
     9301 tcgaaagcat tgaaacatta aaacttccag aatttcagat atatggtgga accttcaaaa
     9361 tcatcaatac ttattttgga gataaaaaac gatatttaca agcaattaaa gaattggagc
     9421 aagagctatt tacagtagct taatgaaagg aaagtatgtc aattacatca tttgtaaaaa
     9481 gaattcaaga tatcactcga aacgatgctg gtgttaatgg tgatgctcaa cgtattgagc
     9541 aaatgtcttg gttattattc ttaaaaattt atgatagccg tgaaatggtt tgggaattag
     9601 aagaagacga gtatgagtca attatcccag aggaattaaa atggcgaaat tgggctcatg
     9661 ctcaaaatgg ggaacgggta ttgacaggcg atgaattact tgattttgtc aataacaagt
     9721 tattcaaaga gttgaaagag cttgaaataa cttcaaatat gcctattcga aaaacgattg
     9781 ttaaatcagc ttttgaagat gcgaacaact atatgaaaaa tggcgtcttg ttacgccaag
     9841 tcatcaatgt tattgatgaa gttgatttca atagccctga agatcgtcat tcgtttaatg
     9901 atatttacga aaaaattctt aaagatattc aaaatgctgg gaactcagga gaattttata
     9961 cgccacgtgc agcgactgat tttattgccg aagttcttga cccaaaactt ggagaatcaa
    10021 tggcagacct tgcttgcgga acaggaggct tcttgacttc gactctgaac cgtttaagta
    10081 gtcaacgtaa aactagtgaa gataccaaaa aatataatac agctgttttt ggtattgaaa
    10141 agaaagcatt tcctcatctt ttagcagtta caaatctgtt tcttcacgaa attgatgacc
    10201 ctaaaattgt tcatggaaat actttggaga aaaatgttcg tgaatatacg gatgatgaaa
    10261 aatttgacat tattatgatg aatccacctt ttggagggtc agaattagaa acaataaaaa
    10321 ataactttcc agcagaatta cggagttctg aaacagctga tttatttatg gctgtcatta
    10381 tgtatcgttt gaaagaaaat ggtcgtgttg gagttatttt acctgatggt tttctatttg
    10441 gtgaaggtgt aaaaactcgc ttgaaacaaa aactggtaga tgagttcaac ttgcatacga
    10501 ttattaggtt gcctcatagt gtctttgcac cgtatacagg aatccatacg aacattcttt
    10561 tctttgataa aacaaagaaa acagaagaaa cttggtttta tcgtttagat atgccagatg
    10621 gttataaaaa tttctcgaaa actaagccga tgaagtcaga acacttcaat cctgttcgtg
    10681 actggtggga aaatcgtgaa gagattctgg aaggtaagtt ctacaaatct aaatcattta
    10741 cacctagtga attggctgag ttgaattata atttagacca gtgtggtttt ccaaaagagg
    10801 aagaggaaat cttaaatccc tttgagttga ttcagaatta tcaagcggaa agagcaactt
    10861 taaatcataa gattgataat gtattagctg atattttgca gttgttggag gacaaataat
    10921 gacaccagaa caacttaaag caagtattct ccaaagagcg atggaaggga aattagtgcc
    10981 gcaaaatccc aatgacgaac ctgcaagtga attattaaag agaattaaag ctgaaaaaga
    11041 aaaacttatc agtgaaggaa aaatcaaacg agataaaaag gaaactgaga tatttcgtgg
    11101 tgatgatggg aaacattatg ggaagtttgc tgatggaagt actcaagaaa ttgatgttcc
    11161 ttatgatatt cctgatactt gggagtgggt gaggttttct acattggttg aaattgtcag
    11221 aggtggctct ccacgaccaa tcaaagatta tcttacttct gaagtagatg gaataaattg
    11281 gataaaaata ggtgatactg aaaagggtga aaagtatata aataatgtta aagaaaaaat
    11341 caaaaaatca gggcttaaca aaactagatt tgtaaaaaaa ggtacatttt tgttaactaa
    11401 ttctatgagt tttggtagac cttatatttt gaatgttgat ggtgcaatac acgatggatg
    11461 gttggctatt tcgaactatg aaaactcatt aaataaagat tacctattct atattctttc
    11521 atcaaatgta gtttattctc aatttctatc tctaattagt ggagctgttg tgaaaaactt
    11581 gaatagtgat aaagttgctt ctattcttat ccctctcccc ccactatccg aacaacaacg
    11641 cataatagaa gcaatcgaat cagctttaga aaaagtagat gaatatgctg aaagttataa
    11701 tagactagaa cagctagata aagaatttcc agataaacta aaaaaatcta ttcttcaata
    11761 tgctatgcaa ggaaaattag ttgaacaaga cccaaatgat gaatcagtcg aagttttact
    11821 tgaaaaaata cgagcagaaa aacaaaaact ctttgaagaa ggcaagatta aaaagaaaga
    11881 tttggacatt tctattgttt cccaaggaga tgataactct tattatgagg aagtaccttg
    11941 tgaaatacca gaaagttggg aatgggtgag gttaaacgat ataacctcct acattcaaag
    12001 agggaagtct ccaaaatatt caaatattcc tatataccct gtgattgctc agaaatgtaa
    12061 tcaatggtct ggcttttcta ttgacttagc aagatttatt gatcccgaaa cagttcatag
    12121 ttaccagaaa gaaagattac taagagatgg tgatttaatg tggaattcaa caggtctagg
    12181 tacgttagga cgattagcta tttaccatga aaataaaaat ccttatgtct gggctgttgc
    12241 agatagccat gttacagtta taagagtctt atcgggagtt attaattgcc atttcatcta
    12301 taattttctt tcctctccta ttgttcagtc agttattgaa gaaaaagcgt cgggtagtac
    12361 aaaacaaaaa gaattattaa cgaaaactat aaaagaatat ttaatccccc tcccacccct
    12421 tcctgaacaa tccagaatcg ttgataaaat cgaacaattt ttcgcccata ttgacgcact
    12481 aatttagtat aatagtttta cgtgcggtgg aatttctatc aaacataaga taggagtttt
    12541 acgttgaaag attttgaatt acatttgaag aaagctggtt tagctgaaaa tactgtgcgt
    12601 tcgtaccttt atggtgttcg atttttcttg gagaattacg aactcaaaat ggaagattta
    12661 tttgaataca aaggatattt attagacaat tttaaaccta aaactgtcaa tttgcgacta
    12721 caaggagtaa ataaatatct ggcttttatt ggtcatgatg atttgaaatt aaaatttgtc
    12781 aaagtacagc aaaaaccgtt tttagaagat gtcattagcc atgctgatta cctttttctt
    12841 aaacgtagtt tgaaaaaaga tggtatttta aaatggcatt ttgtcgtttg gtttttaggt
    12901 gcaactgggg ctcgtgtaag tgaacttatt aaactaaaag tagagcatgt agaaatcggt
    12961 tattttgaca tttactcaaa aggtggtaaa attcgtagac tgtacattcc taaaaaatta
    13021 agaaatagtt gtcttagttg gttagaatca gaaaatcgcc gaagtggtta tttatttttg
    13081 aataaattta acgaaccaat tacagcaaga ggagttgctc aacagttaaa aaattatgct
    13141 gataaataca aaatgaatcc taaagtaatt taccctcatt cttttaggca tttatttgct
    13201 aagaattttt tagcgaagta taatgatatt gccttgcttg cagatttgat gggacacgaa
    13261 agtatagaaa ctactcgaat ttatctaagg aaaacagcta ctgaacaaca aaatattgta
    13321 gataaaattg ttaattggta aaaaataaca ggtggtcaaa ctgactacct gctatttttg
    13381 tgattatggc tcttattatg ggaacaaaga cgaaacaacc tcttatccta tatatgaaat
    13441 cccagaagca tggagatata ttaaatttgc tagcctagtt aacttcagaa taggaaaaac
    13501 tcctccacgc agtgaagcta ctttttgggg aactgaaata ccttgggtat ctatatcaga
    13561 tatgcctata tctggttatg taactaatac gagagaatct atttctaaat tagcattaaa
    13621 atcaaagaaa atagacattt cacctaaagg aactttatta atgagtttta aattatctat
    13681 tggaaaagtt gccatattgg atattcctgc cactcataat gaagccatta tatcgatatt
    13741 cccctatgct aataaagaaa atattatcag agattactta atgatatttt tgccacttat
    13801 ctctacttta ggtgattcaa aagatgctat caaagggaaa acattaaata gtaccagtat
    13861 ctccgaatta ttaatcccta tttctaatca tgaagagatg aaaagaatca ttttcaaagt
    13921 tgatttactt tttcaaaaag tttctcaact ttttgagtaa taagttcctg ttcctcaaaa
    13981 ggagctaacg gaattaatag ctcgctcagt gtagttttag gaatattata taaagcttga
    14041 cctgatagtt tagttattgc tttcaattgt ttataaaata acggagagga caagttaaat
    14101 aatagaaatt ttgaaataat ctctgaactt tcgaatggtg ttaattggaa aataaatcca
    14161 ccagccacaa caccatcata gtctttatcg attcttgcaa actttccaat atgttctaaa
    14221 gaggttgcta caggtgttat tagctgatta tgttttaaat aaacttgctc agaggagatg
    14281 aattgtgtat caatgtagta atcattatcc aacagagaaa attctaaagg cttaatatta
    14341 ccaccacgta taattctaac acctttatta ttaatgctta aatcgccctt cttgtaagaa
    14401 agacctgtat tcattgaaaa aatatctttt atttttataa caacccaatt cataggtata
    14461 ttc
//
LOCUS       DBERDK010000044        14443 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.044, whole genome shotgun sequence.
ACCESSION   DBERDK010000044 DBERDK010000000
VERSION     DBERDK010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 14443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14443
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.044"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(120..1409)
                     /gene="hisS"
                     /locus_tag="ACL73L_001392"
     CDS             complement(120..1409)
                     /gene="hisS"
                     /locus_tag="ACL73L_001392"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HGS0772354.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIAMAAHFLKEIGIQG
                     VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVA
                     VENAPSILDFLDEESQAHFDAVRQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQGVALPIENALDVYIA
                     VLGDGANVKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFAAKTLITLGESEVES
                     GQVTVKNNQTREEVQVSLETISQNFSEIFEKLGFYTQ"
     gene            complement(1487..2734)
                     /locus_tag="ACL73L_001393"
     CDS             complement(1487..2734)
                     /locus_tag="ACL73L_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HGS0772355.1"
                     /translation="MKLLFRNPAYRILTLSRFFNAFGASIFNLVFIVYASTLSQASFA
                     VAMANIVMILPTFFTVFAGIRADYTRDKVKWMVYSGLFQAVLFFLAALVVQQASLFAF
                     SSLCLINVISDIISDFAGGLRMPLIKEKVAEDDLMEAYSFSQFITYISAIGGQAFGVW
                     LLALSVNNFSLVAGINACFFLVSATILFLGKSKLSLSMSSADGENLKNEKFSIKDQFL
                     TIYRNLRLVFLKSGQKNFGFMLFAVLLINSLGGALGGIYNIFFLSHSLLNFSYTEALF
                     INQFCVLVAIIISSLTGNDYFGKQSLPRLMMWATVGLSLVGLANLFNQVVLGLLFLFF
                     TLYVSGKVQPKISAMLMKNLAPEVLARTSNFLDLLFTLSIPVGTACFSLVAVWNMQLT
                     WMLFVGLSLLAIFLTILNLKNDI"
     gene            complement(2925..3776)
                     /gene="rgg"
                     /locus_tag="ACL73L_001394"
     CDS             complement(2925..3776)
                     /gene="rgg"
                     /locus_tag="ACL73L_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434947.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
                     /protein_id="HGS0772356.1"
                     /translation="MEQIGKVFRQLRESRNISLRQATGGQFSPSMLSRFETGQSELSV
                     EKFLFALENISASVEEILFLARGFQYDTDSELRKEITDVLEPKNIAPLEDLYRREYQK
                     HAHSHNKQKHILNAIIIKSYMKSIDERVELTAEEGKVLHDYLFSTEIWGIYELNLFSV
                     SSPFLSVSLFTRYVREMVRKSDFLMEMSGNRNLFHTILLNGFLASIECEEFTNAYYFK
                     RVIEEHFYKENETYFRIVYLWAEGLLDSKQGRVKEGQKKMEDAVRIFEMLGCNKSAEY
                     YRNTTEC"
     gene            3900..4241
                     /locus_tag="ACL73L_001395"
     CDS             3900..4241
                     /locus_tag="ACL73L_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HGS0772357.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIKDIKVEVTWSPAWKITRISR
                     YGRIALGLPPR"
     gene            4305..4469
                     /locus_tag="ACL73L_001396"
     CDS             4305..4469
                     /locus_tag="ACL73L_001396"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772358.1"
                     /translation="MKIKGQTRKLAAGCSKHCFEVMDRTDEVSPKHCFEVVDRTDEVS
                     NHTYGKATLT"
     gene            complement(4495..6198)
                     /gene="ilvD"
                     /locus_tag="ACL73L_001397"
     CDS             complement(4495..6198)
                     /gene="ilvD"
                     /locus_tag="ACL73L_001397"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HGS0772359.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETSIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKRADGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIIDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQ
                     DTKEISMAVSEEELEKRKAETTLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGK
                     K"
     gene            complement(6431..7363)
                     /locus_tag="ACL73L_001398"
     CDS             complement(6431..7363)
                     /locus_tag="ACL73L_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HGS0772360.1"
                     /translation="MMRSTKELRYVYRDFLLEANQSYSDIVVLEADLSSSMATHNLEK
                     DFGDRYVNVGIMEAEMVGLAAGLSIQGFRPYLHTFGPFSSRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIPKSIIFEATDDIQFREILNQTLDLKGLK
                     YIRTIRKAPEAVYQGGEDFSKGYIELRHGEDLVIVASGIMVAPSIRVADELSKLGYSV
                     GVIDLFRIKPIPEQIKTMLSGKTIFTVENHNQIGGIGSALCELFSDDGITKIHRMGVQ
                     ERFGQVGKMDYLLNEYGLSESNIKEKVLEIYNAR"
     gene            complement(7360..8217)
                     /locus_tag="ACL73L_001399"
     CDS             complement(7360..8217)
                     /locus_tag="ACL73L_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HGS0772361.1"
                     /translation="MILSKNREDELRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNPGDFVEKFSAFGFESIRVKGSDIR
                     EIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQELEEMKSNHHLRPTVEERQMLTSVVER
                     LSQELEETE"
     gene            complement(8221..9567)
                     /locus_tag="ACL73L_001400"
     CDS             complement(8221..9567)
                     /locus_tag="ACL73L_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HGS0772362.1"
                     /translation="MMKFILDIVSTPAILVALIAILGLVLQKKKLPDIIKGGIKTFVG
                     FLVVSGGAGIVQNSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATAMIMFAGM
                     VFNILIARFTRFKYIFLTGHHTLYMACMIAVILSVAGFTSLPLILLGGLALGIIMSIS
                     PAFVQKYMVQLTGNDKVALGHFSSLGYWLSGFTGSLIGDKSKSTEDIKFPKSLAFLRD
                     STVSITLSMAVIYIIVAIFAGSEYIEKEISSGTSGLVYALQLAGQFAAGVFVILAGVR
                     LILGEIVPAFKGISERLVPNSKPALDCPIVYTYAPNAVLIGFISSFIGGLVSMVIMIA
                     SGTVVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFLQGILISFLPVFLMPVLGGL
                     GFQGSTFSDADFGLSGIILGMLNQFGSQAGIVIGLVLILAVMFGVSFIKKPSAKEE"
     gene            complement(9580..9864)
                     /locus_tag="ACL73L_001401"
     CDS             complement(9580..9864)
                     /locus_tag="ACL73L_001401"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGS0772363.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELITKLCEEKGLI"
     gene            complement(9868..11898)
                     /locus_tag="ACL73L_001402"
     CDS             complement(9868..11898)
                     /locus_tag="ACL73L_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HGS0772364.1"
                     /translation="MFDYRALVILMTLMDHCEVSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NNHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTYDLIDQVVKDYYEKLQLVPIVNQLKVYLFGLVF
                     ILCRYQRDVERVCLSETLVSPVIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEHYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKCYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKASSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKRYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYNDIKMIIKQED"
     gene            12090..13097
                     /locus_tag="ACL73L_001403"
     CDS             12090..13097
                     /locus_tag="ACL73L_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HGS0772365.1"
                     /translation="MTEKLINSKPNGVFALILIELTLVLGIFIFIMGVGSENIFGIII
                     GPLLIVIAGLAHAGLKVVKPQEALVLTLFGNYTGTIKEPGFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKSPFLGAKSSNDNDVNLEIGKKHISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQSRVEDAGLEILEARITYLAYAPEIAAVMLQRQQASAIIDA
                     RKMIVDGAVGMVEMALERLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            13100..13300
                     /locus_tag="ACL73L_001404"
     CDS             13100..13300
                     /locus_tag="ACL73L_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HGS0772366.1"
                     /translation="MAEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYVSETIDEPFEIDI"
     gene            13452..13634
                     /gene="rpmF"
                     /locus_tag="ACL73L_001405"
     CDS             13452..13634
                     /gene="rpmF"
                     /locus_tag="ACL73L_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HGS0772367.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKITKAASAE"
     gene            13650..13799
                     /gene="rpmG"
                     /locus_tag="ACL73L_001406"
     CDS             13650..13799
                     /gene="rpmG"
                     /locus_tag="ACL73L_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HGS0772368.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(14291..>14443)
                     /locus_tag="ACL73L_001407"
     CDS             complement(14291..>14443)
                     /locus_tag="ACL73L_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772369.1"
                     /translation="WYYLNESGSMATGWFTVSGKWYYTYNSGDLLVNTTTPDGYRVNA
                     NGEWVG"
BASE COUNT         4647 a         3058 c         2480 g         4258 t
ORIGIN      
        1 gtcagtttca tctacaacct caaaaccatg ttttgagctg acttcgtcag ttctatccac
       61 aacctcaaaa cagtgttttg agcaacctgc ggctagcttc ctagtttgct ctttgatttt
      121 cattgagtat aaaatcctag tttttcaaag atttctgaga agttttggct gattgtctca
      181 agtgacactt gcacttcttc tcgggtttgg ttgttcttga ccgtcacttg tccgctttcg
      241 acttcgctct ctcctagggt gatgagggtc ttagccgcaa agacatctgc tgacttgaac
      301 tgagctttga gcttacggtt gaggtaatca cgctctgctt tgaaaccttg ttggcgaaga
      361 gcttggacta gttctagggc tttgacattt gctccatcac ccaagactgc gatatagaca
      421 tctagggcgt tttcgatagg gagggccacg ccttgttttt caaggatgag aagcaggcgc
      481 tctacaccaa gcccaaaacc aaatccagca gtttcagggc ctccaaagta agcaaccaaa
      541 ccatcgtagc gaccacccgc acagactgtc aagtcattgc cctcaatctc tgtgataaac
      601 tcgaaaatgg tgtggttgta gtagtccaga ccacgcacca tattggtatc gatgatgtaa
      661 tctactccaa gattttccaa catctgacgc acagcatcaa aatgagcttg gctttcttca
      721 tcaagaaagt ccaagataga cggcgcattc tctactgcta ccttgtcttc tttttcctta
      781 gagtccaaga cacgaagagg attttcctcc aagcgacgtt ggctatcctt agacaaggtc
      841 tccttgagcg gtgtcaaata gtcaatcaag gcttggcggt aggctgcacg gctctcagga
      901 tttccaagag tgttgaggtg caatttgaca ccttgaatac cgatttcctt caaaaaatgg
      961 gctgccatag cgattgtttc cacatcggta gctggattgc tagagccaaa acactcaaca
     1021 ccaatctggt ggaattggcg caagcgccct gcctgtggac gctcataacg gaacataggt
     1081 cccatgtagt agaacttgct tggcttttgc acttctgggg cgaagagttt attttccaca
     1141 taggaacgga caacgggcgc agttccttct ggacgaaggg taatatggcg gtcacccttg
     1201 tcatagaaat cgtacatttc cttggttaca atatctgttg tatctccgac agagcggctg
     1261 ataacctcgt aatgctcgaa aataggcgta cgcacttctg cgtagttgta gcgtttgaaa
     1321 atttcacggg caaagccctc aacgtactgc cacttggcag attcagcagg taaaatatcc
     1381 tgcgttcctt ttggtttttg taatttcata gggaatcctc tttaaactta atagtcttat
     1441 tttaccataa atagagggat taaaacagtg agaaaaaaat taggatttag atatcatttt
     1501 tgagattaag aattgttaaa aaaatagcta gcaaggaaag accaacaaat agcatccaag
     1561 tcaactgcat gttccatacg gctactagtg aaaaacaagc tgttcccaca ggtatggata
     1621 aggtaaacaa tagatctaaa aaattactag tacgagctag aacctctgga gctagatttt
     1681 tcatgagcat ggcactaatc tttggttgaa ctttaccaga cacatacaga gtaaagaaga
     1741 gaaatagcaa accaagcacg acttgattga ataaattagc caaaccaact agactaagtc
     1801 ctacggtcgc ccacatcatc aatctaggca aggactgctt cccaaaataa tcattgcccg
     1861 taaggctact gatgatgatt gctactaaaa cacagaattg attgataaat agtgcctctg
     1921 tataagaaaa attcaagaga gaatggctca aaaagaagat attataaatt ccacccaaag
     1981 cgccacccaa ggaattaata agcaagacag caaagagcat aaaaccaaag tttttctgtc
     2041 cacttttaag aaaaacgaga cgtaaatttc ggtaaattgt taggaactgg tctttgatag
     2101 aaaacttctc attttttaag ttttcaccat cagcagatga cattgacagg ctcaatttgc
     2161 tttttcctaa aaagaggata gtggctgata ctaggaaaaa gcaggcattg attcccgcaa
     2221 cgagagaaaa attgttgacc gatagagcta agagccagac tccgaaagct tgaccaccaa
     2281 tagctgaaat ataggtgatg aactgtgaaa aagaataagc ctccatcaga tcatcttcag
     2341 ctactttttc cttaataaga ggcatacgca ggccacctgc aaaatcactg atgatatcac
     2401 taatgacatt gatcaaacac aggctagaaa aggcaaagag actagcttgc tgaacaacta
     2461 gggctgctag aaaaaataga accgcctgaa acaaaccgct atagaccatc catttgacct
     2521 tgtccctcgt gtaatctgcc cgaatccctg caaaaactgt aaagaaggtc ggaagaatca
     2581 tgacaatatt cgccatagca acagcaaaag atgcttgtga caaggtcgat gcatagacga
     2641 taaagaccag gttgaaaatc gaagcaccaa aagcattgaa gaagcgtgat aaagtcaaaa
     2701 ttctataagc tggatttcta aacaatagtt tcatagataa tctccctcct tgttctaagt
     2761 tctacatcaa gaatagcttt agtatagcac ttatgaattt gcgaaacgat tcttttgaat
     2821 atatgcaaca aacaaaatga gcaaaaaaac gatagaactt ttgtaatgac ctgagagagg
     2881 gcaacagttc tatcgcttca aattttttct tggtttgcag atactcaaca ttcggtcgta
     2941 tttctatagt attcggcaga tttattacag ccaagcatct caaaaatacg gacagcatcc
     3001 tccatctttt tctgaccttc cttgactcta ccttgcttgc tatcaaggag accttctgcc
     3061 cacagataaa caattcggaa ataggtctca ttttccttgt agaaatgctc ttcgataaca
     3121 cgtttaaaat aataggcatt ggtaaattct tcacactcaa tactagctaa aaagccattc
     3181 aatagtatag tatgaaaaag gtttcgattg ccagacattt ccattagaaa atcagattta
     3241 cgtaccattt ctcgtacata tctagtaaaa agagaaacag ataaaaatgg agaactgact
     3301 gaaaataaat tgagttcata gattccccag atctcggtag aaaacaaata atcatgaagg
     3361 acttttcctt cctctgctgt taactctacc ctttcatcta tgctcttcat ataagacttg
     3421 ataataatgg catttagaat atgtttctgt ttgttgtgag aatgggcatg cttttgatac
     3481 tccctgcgat ataagtcctc aagaggtgct atattctttg gttccaagac atctgtaatt
     3541 tcttttctca actcagaatc tgtatcatac tggaaacctc tcgccagaaa gaggatctcc
     3601 tccacactgg cagatatatt ttccagagca aatagaaact tttccaccga aagctcactc
     3661 tgacctgttt caaaacggga caacatagac ggcgaaaatt gtcctccggt tgcttgtctc
     3721 agtgagatat ttcttgactc tcgtaattgt ctaaagactt ttccaatctg ctccatagac
     3781 ttccccttga ttccgtattt tcttcatttt atcatatttt tcagaaaatt catcaaaaac
     3841 ttgccaaatt gtcagaatta tgagaaaata gaggatattt atcacgtgga gggactgcta
     3901 tgagagacga tatcaaaatc aatgaccgtg ctttggcctt gcaagaccaa atcatcgaaa
     3961 aactagagaa agtttttgat acagatgtgg aattggatgt ttacaatcta ggtctgattt
     4021 atgaaatcaa tctggatgaa acggggctct gcaagattgt catgaccttc accgatactg
     4081 cctgtgattg cgccgaaagc ctgcctattg aaatcgtggc aggtctgaaa caaatcgagg
     4141 gtatcaaaga tatcaaggtt gaagttacct ggtcgcctgc ttggaaaatc acacgaatca
     4201 gtcgctatgg ccgtattgcc cttggactac cacctcgtta agcaggccaa tcatttttaa
     4261 agatgaaaaa agcaagccaa aaatggcttg ctttttgata ctcaatgaaa atcaaagggc
     4321 aaactagaaa actagccgca ggttgctcaa aacactgttt tgaagttatg gatagaactg
     4381 acgaagtcag cccaaaacac tgttttgagg ttgtggatag aactgacgaa gtcagtaacc
     4441 atacctacgg caaggcgacg ttgacgtgat ttgaagagat tttcaagtat gagtttattt
     4501 tttacctgac ttgtccatat tccagaagtc tgtcacggct ccgcgtgaag cagatgatac
     4561 gatatgggca tatttaccga ggacaccacg gctgtaaagt ggtggcaagg ttgtttctgc
     4621 cttgcgtttt tcaagttctt cttcggatac ggccatggaa atttctttgg tatcttggtc
     4681 aaccgtaacg atatcgccgg tacggagata ggcaattggt ccaccatcct gagcttcagg
     4741 agcgatatgt ccaacaacca gaccataagt accaccagag aaacgtccgt ccgtcaagag
     4801 ggccacctta tctccctgac ctttaccaac aatcattgaa gaaagtgaca gcatctcagg
     4861 cataccagga ccacctttag gtccaacaaa acgaacaacg actacatcgc catcaatgat
     4921 ttcatctgtc aggacggcct gaatcgcatc ttcttctgag tcaaagacct tagctggccc
     4981 aacgtgacga cgcactttaa cacctgatac cttggcaact gcaccgtcag gagcaaggtt
     5041 cccgttcaag atgataagcg gaccatccgc acgttttgga ttttcaagtg gcatgataac
     5101 tttttggcct ggagtcaagt ctgcaaagtc agccaagttt tcagctacag tcttaccagt
     5161 acatgtgatg cgatctccgt gaaggaaacc atttgccaac aagtacttca taaccgcagg
     5221 gacaccaccg acttcgtaga ggtcttggaa gacatactga ccagatggtt tcaagtcggc
     5281 caagtgaggc acacgttctt gaatcgtatt gaagtcctca agtgacaagt caacatttgc
     5341 ggcatgggca atggcgagca agtgaagagt ggcgtttgta gaaccaccga gagccatcgt
     5401 tacagtaata gcatcttcaa aggcttcacg agtcaagata tctgatggtt tgagaccaag
     5461 ttccaacatc ttaacaacag cacgtcctgc tgcttcgata tcttctttct tatcagctga
     5521 ttcagctggg tgagaggatg accctggcaa actcatccct agaacttcga tagcagttgc
     5581 catggtatta gcagtataca taccaccaca accaccaggg ccagggcagg cattacattc
     5641 aagacgtttc acgtcctcag ctgtcatgtc accgtggttc cattttccga taccttcaaa
     5701 gacagaaacc aagtcgatat ctttaccatc aagatttccc ggtgcaatag ttccaccata
     5761 ggcgaaaata gctgggatat ccatattagc aatagcaatc atagatccag gcatgttctt
     5821 gtcacagcca ccgatagcga cgaaggcatc cacgttgtga ccactcatag ccgcctcgat
     5881 ggagtccgcg atgatgtcac gagatgttag agagaaacgc ataccaggcg ttcccatagc
     5941 gatcccgtcc gctacggtaa tggttccaaa ctgtacaggc caagcgcctg cagatttgac
     6001 accttcttta gccagtttcc cgaaatcatg caagtgaatg ttacatggtg tattttccgc
     6061 ccaagtcgaa atcactccca caatcgatgt ttcaaagtcc ttatctgtca taccagtcgc
     6121 acgaagcata gcacggttag gtgatttaac catgctgtca tagatgctac tgcggtgacg
     6181 tttatctaat tcagtcatct tatccctccc atttcagttt ttactattat agcacaattt
     6241 aaaggcaatg aacagaataa aattcttgaa ttttcagaaa attctatacg aatgtgaaat
     6301 atttaaaatt aaaaacaaca aagcggatta gtgcactttc tgatgaccag aatatgcttt
     6361 ttaatccgct ttctttaaat aacgtaatgt aatttttaca gaaattcttt cagataagtg
     6421 tatttaacat ctatcttgca ttataaattt ctagaacctt ctcttttata ttcgattcac
     6481 tcaaaccata ctcattaaga agataatcca ttttccctac ttgaccaaat ctttcttgaa
     6541 cacccatccg atgaattttt gttattccat catcagagaa taattcacat aaagcactgc
     6601 caattccacc tatctgattg tggttttcta cagtaaatat agtttttcca cttaacattg
     6661 tttttatctg ttctggtatc ggtttgattc taaataaatc tatcacacct actgaataac
     6721 ctaatttaga cagttcatct gcaactcgaa tacttggagc aaccattata ccagaagcaa
     6781 cgattacaag atcttcacca tgccttaact caatgtagcc tttagaaaaa tcttctccac
     6841 cttgatatac agcctctgga gcttttctaa ttgttcgaat atattttagt ccttttaagt
     6901 ctaatgtctg gttcaatatt tcacgaaatt ggatatcatc agttgcttca aaaatgatcg
     6961 atttaggaat taaacgtaac aatccaattt cttcaaatgg catatgtgtc ccaccattca
     7021 tttctgcagt tactcctgca tctgatccaa tcacagtggc atccaattgt gcgtatccaa
     7081 gagaaataaa taattgatca aatacccttc gtgaagaaaa aggaccaaat gtatgaagat
     7141 aaggtctaaa cccctgaata gacaagcctg ctgcaaggcc gaccatttct gcttccataa
     7201 tcccaacatt cacataacgg tctccaaagt ccttttcaag attatgagta gccatcgaac
     7261 ttgacaaatc ggcttctaag actactatat cagaataact ttgattagcc tctaaaagga
     7321 agtctctata tacataccgt aattctttcg tacttctcat cattctgttt cctccaattc
     7381 ctgacttaat ctttctacaa ctgaagttaa catttgtctc tcctctacag tagggcgaag
     7441 atgatgattg gatttcattt cttccagctc ttgaacccct tgacctttaa tagtatctaa
     7501 tacaatgcac ttaggtgatg aattatttga ctgttttaat tggacaatcc cttcataaat
     7561 ttctctaata tctgaaccct tgaccctaat ggattcaaat ccaaatgctg aaaatttttc
     7621 tacgaaatca cctggattac aaatatcctt tgtaaaacca tctaattgtt ttttgttatc
     7681 atcaacaaat acaattaagt tggataactg ttgatgagaa gcaaactgta tagcctccca
     7741 acattgtccc tcatttaact caccatctcc aacaatagca taagtataaa agggactctt
     7801 tcttattctc tgaccatatg caagtccagt tgcaacacta attccttgtc ctaaagagcc
     7861 cgttgtcata tctatgcctg gcgttagatt tctatcagga tgagacggta atttggttcc
     7921 atttgtattt aaagaatata agaattcttt gtcaaagaaa ccattcaaat agagtgtact
     7981 gtatagagct ggtcctccat gaccttttga taatatgaaa taatctctat ctcgtgctgc
     8041 aaatatttct ggagtcattg gcattatttc accataaagc actgctaaaa cttctacgat
     8101 agacagactt cctccgtaat gtccgaatcc aagatgattc aatgttctaa gagtatttaa
     8161 tcggatgttg gtcgcaaatt ttcttaactc atcttctcta tttttactta aaatcatccc
     8221 ttattcctcc tttgcagatg gctttttaat aaaggatact ccaaacataa ctgctaaaat
     8281 aagaacaaga ccaatcacaa tgcctgcttg tgagccaaat tgatttaaca ttcctaaaat
     8341 aattcctgat agaccaaaat ctgcatctga gaaagttgat ccttggaaac cgagtcctcc
     8401 caaaactggc attaaaaaga ctgggagaaa actgattaaa ataccttgta aaaatgctcc
     8461 aatagtggct ccacgaacac caccagatgc attcccaata acacctgcag tcgctccaca
     8521 gaagaaatga ggcacaacgc ctggtaagat aacaaccgtt cctgaagcaa tcataattac
     8581 catacttact aaaccaccaa taaaactaga gataaatcca attagaactg cattaggtgc
     8641 ataagtataa acaatcggac aatccaaagc aggttttgaa ttaggtacaa gacgctctga
     8701 aataccttta aaggctggaa caatttcgcc caaaataagg cgaacacctg ctaaaataac
     8761 aaatacccct gctgcaaatt gacctgctaa ttgtaaagca taaactagac cacttgtacc
     8821 actactgatt tctttttcta tatattctga ccctgcaaag atagctacaa taatgtaaat
     8881 aactgccata gataaagtga tactaacagt actatcacgt aaaaaagcta aactctttgg
     8941 aaatttaatg tcctctgttg attttgattt gtcaccgata aggctaccag taaaaccact
     9001 caaccaatat cccaaagaac tgaaatgacc taaagccacc ttgtcatttc cagttaattg
     9061 aaccatatat ttttgcacaa atgctgggga aatactcata ataataccga gtgctaatcc
     9121 tcctagtaag atgagaggca agctagtaaa gccagcaact gataaaatga ccgcaatcat
     9181 acatgccata tatagagtgt ggtgccctgt taaaaaaata tatttaaatc gagtaaaacg
     9241 agcgattaag atattgaaca ccattcctgc aaacataatc attgcagtag ctgagccata
     9301 tgttgttaaa gctacagcta caattgcttc attattcggc acaacgccag ataaatgaaa
     9361 agcatgctca aacatggtac caaatggatt taaagaattt tgtacaattc ctgcaccacc
     9421 agatacaact aagaaaccaa caaaggtctt aattccacct ttaataatat caggtaattt
     9481 cttcttctga agaactaatc ctaaaattgc aattaaagct actaaaatag ctggtgtact
     9541 aacaatatcc aatatgaact tcatcatgac gctagcctcc tatataagtc ctttttcttc
     9601 acaaagttta gtaattaatt ctcgtagttc atccatatca ataatactat ttaagatacg
     9661 aacatctcca agatgactag ctgaatcagc tagatcacga ccaacaatcc aaatatcagc
     9721 tgcatttgga tctgctccac ctaaatcata atgttcaact tctacatccg aaacattcaa
     9781 atcactcaat acagattcaa tattcatctg taccataaaa cttgaaccta atcctgaacc
     9841 acaagctgta ccaattttta acattatcta atcctcctgt ttaattatca ttttaatatc
     9901 attatagttt tttgatgata ttaaagtttg aacatgattt ttatctctta aaattgttgt
     9961 taaatgtgac aaagccttta aatgactctc attatcaatg gctgcaatac aaatcaacaa
    10021 tcttacctct tgttctggat tatccaataa ataaatcggt tcttccaaaa ctaacattga
    10081 cattcctatt tcattcacac cttcatctgg ccgagcgtga ggaattgcta ctcccttccc
    10141 taaattaata aaaggtccaa actcttctac tttttgaatc attgcctcag ggtagttctc
    10201 agttatctta tcttgatcca aaagcggttt agctgctaaa cgaatcgcct ccttccatcc
    10261 taatttttgc gaactaacct gataggtttc tttggtaatg agttgttcta gcactggtac
    10321 aatttccttt ctatcatttt tttggtaaag ataattcttt aacgccaatc ttaattccaa
    10381 ttcttgtgta ataattccat atcttttgac aatattcagg atttgttcaa tctcaaaatc
    10441 cccatactct aaattcggaa aatcttttaa cactagttct actagttgta ttgcttgctc
    10501 ttcagtcatc ataaccgaaa ctagataatt tggcttttct gtctccacct ttatggtaga
    10561 aaaaaccata tcatagtcac tactagcttt cacctgtaaa tcatcaatct ttgaggttcc
    10621 tataaactca atttgaggaa ataatgctaa tagattctct tttaacatca atgaagaact
    10681 aacaccatta ggacaaatga ttgctgcttt atagcatttt tgaggcaaag tatctgcttt
    10741 ctttaaataa cctccgaaat ggataacaaa atatgctgtt tcactatcag gtatgggatt
    10801 gtcaatagcg tccatcaagg gcatcaaaga atctttgact aattcaaata aatcaggata
    10861 atgttcttta acatgcaata catattcatt tgaactaggt aggccgaact ttaatctata
    10921 gtaagccggt ataaggtggc ggcgaagatt ttctctcaat ccttcccttt gtttaaaatg
    10981 taacaaagaa atatcttcca ttctacttat aatagcctct gttaattgat taaagtaaac
    11041 cggagcaaca tctacttcac cttcaaagca acttgataat aaaacggtga tatagcgata
    11101 atcatcctca gaaaacaccg tatctataat tcccaaatca accactgtat ccaataaaat
    11161 agtcgttata tcttgaataa caggagatac taatgtctct gaaagacata ctctttcaac
    11221 atccctttga tacctacaca gaatgaatac taaaccgaaa aggtaaactt ttaattgatt
    11281 aacaataggt actagctgta gcttctcata ataatcttta actacctgat caatcaaatc
    11341 ataagttaat gaataccccc aactggataa aacataatcc aaaccccaaa tccctatgga
    11401 ggattccagc aactcactaa ccatttgaaa agctaaacgg tgcttattcc actctgaacc
    11461 gtgtaaagta taaccttttg ctctactgta ccctagctcc aaatcattat ctaacataat
    11521 ctttcttaat gattgaatat cagataaggt tgtattctta cttactttca aaaagtcttg
    11581 gtaatgatta ttcgatataa aatctaatcg gcaaaaagtg taaagataga ttaaagctaa
    11641 gcgagtcgac tttggtaaaa ccaattcatc cgacttaata atatctgtca aagactgctt
    11701 cgtacgattt gataaactat agcgaccttg ctttttatcc agcactatcc ctttattagc
    11761 taaataaggc actaaataat ctattccttc tttgacttcc tttataggta agctcacctt
    11821 aacagataac tcatataatg atacctcaca atgatccatc aaagtcatca aaataactag
    11881 tgctctataa tcaaacattt ctcttccttt ctaactacaa ttatagtgta aaagttataa
    11941 gaataaaaga gtatttgagc acaatataat ctcacctttt aaaagaaatt attgggctga
    12001 aagtttttag tgattatttt tcaactttct attgactttt aaaaacaaag tgatatcatt
    12061 tagatatcaa ataatgggag gtcattctta tgactgaaaa actaatcaat tcaaaaccaa
    12121 atggtgtatt cgcattgatt ctcattgaat tgacgctcgt acttggtatc tttatattta
    12181 taatgggtgt tggttcggaa aacatttttg gaattattat cggaccttta ctaatcgtaa
    12241 ttgcaggtct agctcatgct ggtttaaaag ttgtcaaacc tcaggaagct ctggttctga
    12301 cactctttgg taattataca ggtaccatca aagaacctgg tttttacttt gtcaatccct
    12361 tcagcgtagc agtcaaccct gcaaaccaca ctcgacttgg acaaagtggt gatgttagca
    12421 caaaatctcc ttttttaggt gctaaatcat caaatgacaa tgatgtaaat ctggaaattg
    12481 gcaagaaaca catttccctc aaagtcatga ccttgagcaa ttctcgtcaa aaaatcaatg
    12541 attgcttagg aaaccctgta gaaatcggta tcgcggtaac ttggagagtt gtcgataccg
    12601 ctaaggcagt cttcaacgtt gataactaca aagaatatct ttcattgcaa tgtgatagcg
    12661 ccctccgtaa tattgtccgc atctatcctt acgatgtgtc tcctaatgtg gatactacgg
    12721 gtgacgggca agcagatgaa ggtagtctcc gtggctctag cgaaattgtt gctaaccgta
    12781 ttcgtgaaga aatccaaagt cgtgttgagg atgctggctt ggaaatcctt gaagcacgta
    12841 tcacttacct agcttatgct ccagaaattg ctgccgttat gcttcaacgc caacaagcat
    12901 ctgccattat tgatgcacgg aagatgattg tagatggtgc tgtaggaatg gttgaaatgg
    12961 cactagaacg gctcaatgaa ggggaattgg tagaacttga cgaagaacga aaagctgcca
    13021 tggtttcaaa tctccttgtc gttctttgtg gcaatcatga tgcacaacca attgtcaaca
    13081 caggaagtct ttactaaaga tggctgaagc taaaaaaaag cagatccccc ttcgactctc
    13141 aacaaagtta tacgctgcac tcgcatcatg ggcagaagac gactttcgtt cagtcaatgg
    13201 gcaaatcgaa tatctcctca cagagtgtgt caagcaacgg aagaaagacg gaaaatacgt
    13261 atcagaaacc attgacgaac catttgagat tgatatttaa caccttctcc tgtctgccgt
    13321 gtcggatagg agaatttttt catctttttt tgtcaagtaa ctttacttta caaaaaaaat
    13381 gtgttatcct agtatggttg atgaagatca gtagattgaa tcgaatataa atacctaaag
    13441 gagaaatcaa aatggcagta cctgcacgtc gcacttcaaa agcgaagaaa aacaaacgtc
    13501 gtacacacta caaagtaaca gctccatctg taaactttga cgaaactact ggagattact
    13561 cacgttctca ccgtgtatca cttaaaggat actacaaagg acgtaaaatc actaaagctg
    13621 catcagctga ataatagaag ggagatacca tgcgcgtaaa tattacactt gaacacaaag
    13681 aatctggtga acgcttgtac cttacttcta aaaacaaacg taacactcca gaccgccttc
    13741 aattgaagaa atactcacca aaacttcgca aacacgttgt gtttacagaa gttaagtaag
    13801 cattcaatac gaatcaatac agaagaaaaa cgcttgatca gcgttttttc tttttgttaa
    13861 tttcactata ttgcaaggga aatcattcga atgactacct ttttcactac cttttttgaa
    13921 ataagaatac ggattcggac aattaaaagc aagaggaact caaatgtaag ttggggttcc
    13981 ttttttgtat atttttcaaa taacgcctct acactatttg tagcaaattc accaactaca
    14041 gttgtatctt agttaaaata agttagaata tgtaagtgag taccagatat accaagacat
    14101 cgtcaccatc taaggtatat tcaaaataca aaagttgacc aactagattt ctgaatatcc
    14161 ttatatatcc attcttaaaa ttggtttaaa tagcgtagtc ttttaaacta gttttgagaa
    14221 tccaaaaaat cttcctacat atgtaagaag attttttagt tcagaatgat tagatttagc
    14281 taatggatac ctatcctacc cactcaccgt tagcattgac tcgatagcca tcgggtgtag
    14341 tcgtgtttac taataaatct cctgaattat aggtatagta ccatttacca gaaactgtaa
    14401 accaaccagt agccattgaa cctgattcat ttaagtaata cca
//
LOCUS       DBERDK010000045        14316 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.045, whole genome shotgun sequence.
ACCESSION   DBERDK010000045 DBERDK010000000
VERSION     DBERDK010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 14316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14316
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.045"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(48..791)
                     /gene="srtA"
                     /locus_tag="ACL73L_001408"
     CDS             complement(48..791)
                     /gene="srtA"
                     /locus_tag="ACL73L_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HGS0772370.1"
                     /translation="MSQKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIVW
                     NTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWDAQKLPVIGGIAIPE
                     LEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLASHHIFGVDNANKMLFSPLDNA
                     KNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDLAATERIIV
                     KGDLKETKDYSQTSNEILTAFNQPYKQFY"
     gene            complement(791..3259)
                     /gene="gyrA"
                     /locus_tag="ACL73L_001409"
     CDS             complement(791..3259)
                     /gene="gyrA"
                     /locus_tag="ACL73L_001409"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001152996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HGS0772371.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDAVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIVLRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMVGQVLSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLDEDESIQTVINVESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLAEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLVITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVMIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEA"
     gene            3453..4439
                     /locus_tag="ACL73L_001410"
     CDS             3453..4439
                     /locus_tag="ACL73L_001410"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HGS0772372.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALAFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGQYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQASAKELQAIIDEAWKNPEFQEASKN"
     gene            complement(4740..5483)
                     /locus_tag="ACL73L_001411"
     CDS             complement(4740..5483)
                     /locus_tag="ACL73L_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HGS0772373.1"
                     /translation="MNKKWLFADYYDTTIILLALISVILVLLGFAEMIDLDNPPYSII
                     DLVIWGVFVIDYSWRFFTTKRKWRFILENIFDLLAILPLNAIFTVFRLGRIFRLAKLT
                     KLLKLTRLLRIIGLTGKLERKISRFLRTNGLIYILYVNIFIVLVGSSILSVVEEKSFS
                     NSLWWALVTVTTVGYGDIVPASIFGKWLAVLLMLVGIGTIGMLTSALTNFFVKDNPDE
                     QIKLDKLKDELSSQRILLEKQSEKIEELH"
     gene            complement(5530..5784)
                     /locus_tag="ACL73L_001412"
     CDS             complement(5530..5784)
                     /locus_tag="ACL73L_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGS0772374.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQTNVLEDPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHRRDIY"
     gene            complement(5786..6028)
                     /gene="relB"
                     /locus_tag="ACL73L_001413"
     CDS             complement(5786..6028)
                     /gene="relB"
                     /locus_tag="ACL73L_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HGS0772375.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE"
     gene            complement(6119..6928)
                     /locus_tag="ACL73L_001414"
     CDS             complement(6119..6928)
                     /locus_tag="ACL73L_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HGS0772376.1"
                     /translation="MSKIGFKYSILASGSSGNSFYLETSKKKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI"
     gene            complement(6930..8279)
                     /gene="vicK"
                     /locus_tag="ACL73L_001415"
     CDS             complement(6930..8279)
                     /gene="vicK"
                     /locus_tag="ACL73L_001415"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HGS0772377.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQI
                     NQKVKDLIAGDYSKVLDMQGGSEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     HLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            complement(8272..8976)
                     /gene="yycF"
                     /locus_tag="ACL73L_001416"
     CDS             complement(8272..8976)
                     /gene="yycF"
                     /locus_tag="ACL73L_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HGS0772378.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKALLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            complement(9032..10207)
                     /gene="mutY"
                     /locus_tag="ACL73L_001417"
     CDS             complement(9032..10207)
                     /gene="mutY"
                     /locus_tag="ACL73L_001417"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HGS0772379.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATAPEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     FIEVDNFSQEEQFDLFHQVAEESVNFGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQAYAQANLDSSQD"
     gene            complement(10535..12205)
                     /locus_tag="ACL73L_001418"
     CDS             complement(10535..12205)
                     /locus_tag="ACL73L_001418"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HGS0772380.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELGIDQRR
                     ILWKRVVDLNDRALRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKRR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKTPNLPTSPDAVVIVATLRAL
                     KMNGGVAKDALTEENVEAVRAGFANLKRHVENIRKFGIPAVVAINEFVSDTEAEIAVL
                     KELCASIDVPVELASVWADGAEGGVALAETVVKTIAENPANYKRLYDNDLSVQEKIEK
                     IVTEIYRGSKVNFEKKAQTQIAQIVQNGWDKLPICMAKTQYSFSDNPNALGAPENFEI
                     TIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF"
     gene            12435..13124
                     /gene="coaB"
                     /locus_tag="ACL73L_001419"
     CDS             12435..13124
                     /gene="coaB"
                     /locus_tag="ACL73L_001419"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HGS0772381.1"
                     /translation="MKILVTSGGTSEAIDSVRSITNHSTGHLGKIITETLLSAGHEVC
                     LITTKRALKPEPHPNLSIREITNTKDLLIEMQERVQDYQVLIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQAKISSTDEVQVLFLKKTPKIISLVKEWNPTIHLIGFKLL
                     VDVTEDHLVDIARKSLIKNQADLIIANDLTQISAGQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
     gene            13136..13687
                     /gene="coaC"
                     /locus_tag="ACL73L_001420"
     CDS             13136..13687
                     /gene="coaC"
                     /locus_tag="ACL73L_001420"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HGS0772382.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLKKQGHQVTVLMTQAATEFIQ
                     PLTLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLIAPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETIDEKTL"
     gene            13671..14234
                     /locus_tag="ACL73L_001421"
     CDS             13671..14234
                     /locus_tag="ACL73L_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HGS0772383.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGVTLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILTLAIVPRLQTLKK"
BASE COUNT         4271 a         3189 c         2481 g         4375 t
ORIGIN      
        1 tgaaaatctt aaaaaatcag tgaaatcatg gatttcactg atttgtatta ataaaattgt
       61 ttatatggtt gattgaaagc tgttaggatt tcattagatg tttgtgaata atcttttgtt
      121 tctttcaaat cacctttgac aataatacgt tctgtagcag caaggtcttc acaggttact
      181 aatgtgattt cattgacccc atctctatca tcaacttcat caacacgatc cggtgtcaca
      241 cgtttgactt cacgtatttc ataagtataa actttatttt tatcggttag ataaatcttc
      301 atgccatttt tagcattatc taaaggagaa aataacattt tattagcatt atcaacacca
      361 aagatatggt gactagctag actataattt ccttctccca ttacttgctc gcgtttcatt
      421 gtaccagctc cgtagaagag attaacatta tcaagtcctt taaaaatcgg caaattcatt
      481 tccaattcag gaattgcaat tcccccaata actggtaatt tttgagcatc ccattgagaa
      541 gttagaacag cttccgaaga gatagctttg acagaatcaa agtcaaaatt gccttctgta
      601 tcctgatttt cttctaattt ttcttttgat acctggctaa cttgatactt attggtattc
      661 cagactatga aaatatttcg aatttgagta ttaaaaatca aagccagtga cagtaatatc
      721 agaaatcctg ctaggatatt tgtcagcaaa ttttttcgct tgtttttctt tttattattt
      781 ttttgagaca ttatgcttca ccttctgttt cgttttctgt cccaacttct tctttttctg
      841 ccaccgcaac cgttgtgaaa gtcactatct gagcatcttg atccaggcgc attactttaa
      901 ctcccatagt tgcacgtcct gtttgtgaaa tattggcaag attggttcga atcatgacac
      961 ctgtatcagt gataatcatc aaatcctcat ccccttgaac agtcataaga ccggccagca
     1021 agccattttt ttcggtaatt ttagctgtct gcattccctt accaccacga ccttttgttg
     1081 ggtattcagt agcgactgta cgcttaccat atcctttttc tgtgataaca agaacctcat
     1141 cttgatcagt aatcaagctg gcaccaacaa ctgtgtctcc ttcacgaagg ttaacacctt
     1201 tcacaccagt ggcgatacgg ctcataccac gaacggctga ttgattaaag cgaactgcat
     1261 aaccaaactt ggtaccaatg ataatatcca tatctccttc tgccaacaag acattgatta
     1321 actcatcttc atcctttaaa ttcagcgctt tgagaccatt ttgacgaata ttggcaaact
     1381 ccttaacact ggttctcttc acaataccgt gacgggttgt aaagaagaga taagcatcat
     1441 cactgcgatc agactcaaca ttgataaccg tctgaatact ttcgtcttca tccaatttca
     1501 agagattgac tactggtagc cctttggcag tccgaccata ctcaggaatt tcataacctt
     1561 taagacgata gacacgtccc ttgtttgtga agaagagcag atgatcatgg gtgctagttg
     1621 acactaactc acgaacaaag tcatcatctt tcactcccgt tccttggaca ccacgacccc
     1681 cacgtttttg agcagtgaac tcgtcctgat ccaaacgctt aatgtagcct ctgttagaaa
     1741 gggtaatcaa gacatccgat tcttcaatca agtcctcatc ctcgagactc aagacctgtc
     1801 caaccatcaa ctctgtacgg cgtttatcag aaaatttacg tttaacttca tccaattcgt
     1861 ctttgataat ttgagaaaca cgttcaggct tagcaagaat atctgctaaa tccgcaatca
     1921 gagccaagag gtcatcatac tcagattgaa tcttatcgcg ttccaaacct gtcaaacgac
     1981 gaagacgcat atcaaggata gcttgacttt gacgttcaga aagcttaaac ttgctcatca
     2041 actcagcttg agcttccgca tccgtttcac tagcacggat gatacgaatc acttcgtcga
     2101 tatggtctag cgcaatcaag agaccttcta agatatgagc gcgcgcttcc gctttttcct
     2161 tatcaaaacg tgtacgacga acaaccactt ctttttggtg ctcgatataa gcatccaaaa
     2221 tctgacgaag agacaaaatt ttcggtatac cattttggat agcgagcata ttgaaaccaa
     2281 aattggtttg catttgggtc attttgaaga ggttattgag aataacattg gctgaggcgt
     2341 cgcgcttgac ttcaataaca aatcgaacac cttcacggtt tgactcatca cgtactgctg
     2401 tgataccctc aatgcgtttt tcctgaacca agcgaacaat atgctcatgc accttggttt
     2461 tattgaccat gtaaggaaat tctgttacaa cgatacgctc acgaccagtc ttagtcgttt
     2521 caatctctgt acgagaacgt aggacaatcg aacctttacc tgtttcataa gccttatgga
     2581 tacctgattt ccccatgaca agagcaccag ttggaaaatc tggtccaggc aagacttcca
     2641 tcaagtcctt ggtagtcact tcaggattat ccatgaccaa cttcactgca tcaatggttt
     2701 cacccagatt atgaggtgga atattggttg ccatcccaac cgcgatacca gttgctccat
     2761 taaccaaaag gtttggaaaa cgcgctggca agaccaaggg ttcccgttca ttggcatcat
     2821 agttatcaac gaaatcaact gtatttttgt tgatatcacg aagcatttcc agagcaatct
     2881 tgctcatacg tgcctcggta taacgttgag cggcagcact atctccatcc atggaaccaa
     2941 aattcccatg accatctaca agcatgtaac ggtagctcca ccattgagcc atacggacca
     3001 tggcttcata aatagaggaa tccccgtgtg ggtgatattt acccatgaca tcccctgtaa
     3061 tacgagcaga ttttttatgg ggtttgtctg gggtcacacc caattcattc attccgtaga
     3121 gaatgcgacg gtgaacaggt tttaagccat ctcgaacatc aggaagagct cgcgctacga
     3181 taacactcat ggcgtagtcg ataaaacttg ccttcatctc ctttgtcaga ttgacattca
     3241 ctaaattttt atcctgcatt aataaatgcc tcatttcaca attagtaagt aacaacatta
     3301 taccataaat tcccatctat ttcagcctct aaaccactaa aacgtttaca tcgagaacta
     3361 taaggcatat tcgtgacaaa gttttttaaa agtgatagaa tgaagttgtc tagggaaaat
     3421 cccctaataa aattaaggag atgtttaaaa caatgacttc aactaaacaa cacaaaaaag
     3481 ttatccttgt cggtgatggt gctgtaggtt catcttacgc ttttgcactt gttaaccaag
     3541 gaattgcaca agagcttgga attatcgaaa ttccacaatt gcatgaaaaa gctgttggtg
     3601 atgcgcttga ccttagtcac gcccttgcct tcacttcacc taaaaaaatc tatgcagctc
     3661 aatactctga ctgtgcagac gctgaccttg ttgtgatcac tgcaggtgca cctcaaaaac
     3721 caggtgaaac tcgtcttgac cttgtaggta aaaaccttgc tatcaacaaa tcaatcgtaa
     3781 ctcaagttgt tgaatctggt ttcaaaggta tcttccttgt tgctgctaac ccagttgacg
     3841 ttttgactta ctcaacttgg aaattctctg gtttccctaa agaacgcgtt atcggttcag
     3901 gtacttcact tgactcagct cgtttccgtc aagcacttgc tgaaaaattg gatgtggatg
     3961 ctcgttcagt gcacgcctac atcatgggtg aacacggtga ctctgagttc gctgtttggt
     4021 cacacgctaa catcgctggt gtaaaccttg aagaatttct taaagacact caaaatgttc
     4081 aagaagctga attgattgaa ttgttcgaag gtgttcgtga tgcagcctac acaatcatca
     4141 acaaaaaagg tgcaacatac tacggtatcg cagtagccct tgctcgtatc actaaagcaa
     4201 tccttgacga tgaaaacgca gtacttccac tttcagtatt ccaagaaggt caatacggag
     4261 ttgagaatgt ctttatcggt caaccagctg ttgttggtgc acatggtatc gttcgtccag
     4321 taaacattcc attgaacgat gcagaaactc aaaaaatgca agcatctgct aaagaattgc
     4381 aagctattat tgacgaagca tggaaaaatc cagaattcca agaagcttct aaaaactaat
     4441 ttaactatag acaactcctt gaatcatcag ggagttgttt tttcctgtat ttttttctgt
     4501 attatagtag gttgaagttg aaatagcacc ctatcactct aaaacgttgt tataaaatgc
     4561 tttaaattcc taattgattt gttcagattt tattttattt ctctataaaa cgcatagtat
     4621 caagttttta tacctgataa tatgcgttct attgtgtcaa gacttacttg atttctcctc
     4681 gaatcaagtt ttgttttgtc tcacctttat gatatttttt caactaaatc ttttatcatt
     4741 caatgtagtt cttcgatttt ttcagattgt ttctctagta atattctttg gctcgataat
     4801 tcatctttga gtttatcaag ttttatctgt tcatctggat tatcctttac aaaaaagttc
     4861 gtcaaagcac ttgttaacat ccctattgtt ccaataccga caagcatcag taaaactgct
     4921 agccatttcc caaaaatgga agcaggaaca atatccccat atccaacagt agttactgtc
     4981 acaagagccc accaaagact atttgagaag gatttttctt caacaacgga taaaatcgaa
     5041 ctccctacta aaacaataaa gatattaaca tacaagatat aaatcaggcc atttgtccgt
     5101 aagaatctac taatttttct ttctaattta cctgtaagtc caattattct aagtaaacga
     5161 gtcagtttca gtaattttgt tagtttagct aaacgaaaga tacgccctaa tcgaaacact
     5221 gtaaaaatag catttaaagg gagaatagcc aataaatcaa aaatattttc aagaataaag
     5281 cgccattttc ttttagtcgt aaaaaagcgc caactataat caattacaaa aacaccccag
     5341 attactaaat caataatact ataaggcgga ttgtccaaat caatcatctc agcaaatcca
     5401 agtaatacca gaataacaga gattaaagca agtaaaataa ttgttgtatc ataataatct
     5461 gcaaataacc attttttatt caaattttta ctatccttac tttctatact gttattactc
     5521 agctttaatt caataaatat ctctccgatg accaacttct agagtagcaa cgactaattc
     5581 atcatctaca atttgtacga taactcgata attaccaatt ctatagcgcc attgaccaac
     5641 gcgattacca accaaagcct ttccgtgtcg tcttgggtct tccaaaacat tggtttgtaa
     5701 atagtttgta attagcttct gcgtataacg gtccaatttt ttcaattgct tgatgaaacg
     5761 tcttgttgga actaatttat acaaattatt catccttcaa gcctaaatca tgcatcattt
     5821 cttcccaagt aatgggttca actccttttt ccaagtcttc taaatactct tgataggcta
     5881 aatctgccac acgagcatcg tattcatctt ctagggcttc aagagttttg gtgcgaataa
     5941 gttccgaaag ggaaactcct tcaaacttag ccattgcttt cataaatgtt ttatcagctt
     6001 cagaaacttt taatgtaata gtagtcatct tttgtgctcc cttttttaat ggtaacacca
     6061 ttgtattact ttttaggtgt tcagtcaata taaaaagaac accttctcag cgttctttct
     6121 atatctctgt caatggtgtt gcggtatctg gtgaggtatc ataaacctta aagtctactc
     6181 cgactcccag atcagcttga gccagctgat tgaccatggt catatgagcc agttccttga
     6241 tattgttttc cttagataaa tgcccaaggt aaatcttctt agtacgattt cctagcgtcc
     6301 gaatcatagc ttcagcaccg tcctcgttag aaaggtgacc aaggtcagat aggattcgtt
     6361 gtttgagtcg ccaagcgtaa gaacctgatc gcaaaatctc tacatcatgg ttggcctcga
     6421 taagatagcc atccgcattt tcgacaatgc ccgccatacg gtcactgaca taacctgtat
     6481 ctgtcaaaag gacaaaactc ttgtcatcct tcataaagcg atagaactgc ggtgcgactg
     6541 catcatggct tacaccaaaa ctctcgatgt cgatatctcc aaaagttttg gttttaccca
     6601 tttcaaaaat atgcttttgc gaagaatcca ccttgccaag atatttacta ttttccatag
     6661 cttgccaggt cttttcattg gcataaagat ccataccata cttgcgagcc aaaacgccta
     6721 ctccatggat atgatctgaa tgctcatggg taatcaagat ggcatccagg tcttctggct
     6781 tgcggttaat ttcagctagc agactggtaa ttttcttgcc agacaagcct gcatccacta
     6841 aaagcttctt ttttgaggtt tccagataaa aagaatttcc actggaaccc gacgctaaaa
     6901 tactgtattt aaagcctatt ttactcattc tagtcttcta cttcatcctc ccatacttct
     6961 tctttcactg catccttatc ataagggagc acaatggtaa aggttgaacc cttgccgtat
     7021 tcactcttgg cccaaataaa gcccttatgt tgtttaataa tttctttagc gatagacagt
     7081 cctagacctg taccaccttg tgcacgactt ctagcacgat ccacacgata gaaacggtca
     7141 aagatacgtg gtaaatcctg cttaggaatc cccaaaccgt ggtcagaaat ggataaaatc
     7201 atctggtctt cagttgtctt cattctgaca gtgattttac ccccatctgg cgaatactta
     7261 atagcattat ttaaaatatt gtcgacaacc tgcgtcatct tatctgtatc aatttccatc
     7321 cagatagaat tgatgggata atctctcacc aactcatatt ttttctcctt ttcctgtcct
     7381 ttcatcttgt caaaacgatt gaggataaag gtaataaaag cagtgaagtt aatcagttcc
     7441 acatctaggt gactggtagc attatcaata cgtgaaagat ggaggagatc cgtcaccatg
     7501 cgcatcatac ggttggtctc atcaagagaa accttgataa agtctggtgc tacagtttca
     7561 cacaaagccc cctcatccaa ggcttcaaga taggatttta cgctagtcag aggagtccgt
     7621 aactcatggc taacattgga aacaaagagt cttcgttcgc gttcttcctt ctcctgctcc
     7681 gtcgtatcat gcaaaacagc caccaaacct gaaataaagc cagactctcg acgtatcaag
     7741 gcaaagcgaa ctcgaaggtt caaatattca ccattgatat cttgggaatc tagcaacaat
     7801 tctggacttt gggtaatcaa atcacgcaat tcatagtttt cttctatctt gagcaattcc
     7861 aaaatgcttc tattcagaac atcttcctta accaacccca gttgcttctt ggctgtatcg
     7921 ttaatcatga taatctgacc ccgacggttg gtcgcaagaa ccccatctgt catataaaac
     7981 agaatactat ttagcctctt actctcttgt tctagatttt cctgagtgag acgaataacc
     8041 tccgacaagt cattcaaatt attggtaata ttggtgattt cagacccacc ttgcatatca
     8101 agaaccttgg aataatctcc tgcaatcaaa tctttaacct tttgattgat ttgcttcaac
     8161 tgaatattat cacgtctatt ttccagtaat aagagggtca caacaaggat gaaacctaac
     8221 aaaatcagga taaagataaa atctctggta aaaatggttt gtttcagtaa atcaagcatt
     8281 atttctcatg taatacccta caccacggcg cgtcaagata tactctggtc ggctgggcgt
     8341 atcttcaatc ttctcacgca gacgtcgtac agtcacatca actgtacgga catcaccaaa
     8401 atagtcataa ccccagacag tctcaagcaa gtgttcgcgc gtgatgactt gacctgtatg
     8461 cgatgctaaa tgatacaaaa gctcaaattc acgatgggtt aagtctagtt cttcgccata
     8521 ttttttagcc acgtaggcgt ctggaacaat ttctaaatcc ccaatttgga taggttgagg
     8581 tttactatct gcttcctgac catctactgg cataggttga gaacgacgca gaagagcttt
     8641 aacacgcgcc tgcaactcac gattggagaa gggtttcgtt acatagtcat ctgccccaag
     8701 ttccaaaccg ataaccttat caaattcact atctttggct gaaagcataa gaatgggcac
     8761 actgcttgtc ttacgaatgg tcttagcaac ttctaaacca tcaatttctg gaagcatcaa
     8821 atccagaata ataatatctg gttgctctgc ttcaaattgc tctagcgctt cacgaccatt
     8881 aaaagcagtt acaacttcgt aaccttcctt ggtcatatta aacttgataa tatccgagat
     8941 tggtttctca tcatctacaa ttagtatttt tttcatatgt tcaccttttt ctctactatt
     9001 ataccaaaaa aatagtcaga agacacaata gctagtcttg gctactgtct aagttggctt
     9061 gtgcataagc ctgccagatt ttttgttggg gtttggcaag tgggtaattc ttgaattctt
     9121 ctggtgaaag ccaacgaact tccctatctg aaaaatcatg gaagtcactc acctgacctg
     9181 ctacaatctg tacatgccat tttcgatgac taaaaacatg ctggactgta tcaaaacaaa
     9241 catcaagcca atcaacatct aggtcatagt cctgctggaa actctcttct ggactgggac
     9301 caaagttcac actttcttcc gcaacctgat gaaagaggtc aaactgctct tcttgcgaaa
     9361 agttatcaac ttctataaag gggaaatgcc aaaaacctgc caagagcttt tcgctttcat
     9421 ttttttcaag taaaaattgt ccttgagaat ttttcacaac taaggcttta agataaatag
     9481 gaaccggctt tttcttagga gatttaattg gataacggtc catggttcca ttctgatatg
     9541 ccgcactaaa gtccttgact gggctttctt caggtctggg atttacagga gactcaatat
     9601 cagaccctaa gtccatcaat gcttgattaa aatcacccgg acgatccgga ttaatcaaga
     9661 tctccatcat tgcctgaaaa atttttcgat tacttggaat cccaatatcg tggttgactt
     9721 caaacagacg cgccaagacc cgcatgacat taccatctac agctggctca ggcaagttaa
     9781 aagcaatact ggaaatggct cctgctgtgt aaggtccaat ccctttcaag ctggaaattc
     9841 cttcataggt atttggaaat tggccaccaa agtcagtcat aatctgctgg gctgcagcct
     9901 gcatattgcg aactcgagaa taatagccca agccctccca agctttcagt aaactctcct
     9961 caggcgcagt tgccagactt tcgacagttg gaaaccagtc caaaaatctt tcgtagtaag
    10021 ggataactgt atccaccctg gtctgctgaa gcatgatttc agatacccag atgtgataag
    10081 gatttttact tctcctccaa ggcaaatctc ttttgttttc atcataccaa gcgagaagtt
    10141 tctcacggaa agaaatgact ttctcctccg gccacatgac gataccgtat tctttcaaat
    10201 ctaacatatc tctagtataa cacagaaggt ttcacctgtc tttgtatctg atttataata
    10261 ttttcaatag atagtatata acttttctat ctacttatac tcaatgaaaa tcaaagagca
    10321 aactaggaag ctagccgcag gttgctcaaa acactgtttt gaggttgtgg atagaactga
    10381 cagagtcagt atcatatacc tacggcaagg tgaagctgac gtagtttgaa gagattttcg
    10441 aagagtataa atcttattga tgaactgctt gcagtctgag aaaaaatgag cttggatatt
    10501 atttccaaac tcacttaaag tcaatttcaa tccactagaa caagcctagt acagttccat
    10561 cgctttcaac atccatgttg agagctgctg gacgttttgg aagacctggc atggtcataa
    10621 catcaccagt taaggcaacg atgaagcctg cacctaattt tggtaccaat tcacgaatgg
    10681 taatttcaaa gttttctggt gctccaagcg catttggatt gtctgagaaa ctgtattgag
    10741 ttttagccat acagattggc aatttgtccc aaccgttttg aacgatttga gcaatttgtg
    10801 tttgagcttt cttctcaaag ttcactttgc taccacgata gatttcagtg acaatttttt
    10861 caatcttttc ttggacagaa aggtcgttat catacaaacg tttatagtta gctggatttt
    10921 cagcaattgt cttaacaact gtttcggcaa gtgctactcc accttctgct ccatcagccc
    10981 agacactagc caattcaact ggtacatcga ttgaggcaca gagttctttt aagactgcaa
    11041 tttcagcttc tgtatcagat acaaattcgt taatagctac aactgctgga ataccgaact
    11101 tacggatatt ttcaacgtgg cgtttcaagt tagcaaaacc tgcacgaact gcctctacat
    11161 tttcttcagt cagagcgtct ttagccacac caccattcat cttaagggca cgaagggttg
    11221 cgacaataac aactgcatct ggagatgttg gcaagtttgg tgtcttgata tcaaggaatt
    11281 tctcagcacc aaggtccgca ccaaaaccag cttcagtaac agtgtaatca gccaagtgaa
    11341 gggctgttgt cgtcgccaaa acagagttac agccatgagc gatattggca aatggaccac
    11401 cgtgtacaaa ggcaggtgta ccgtaaattg tctgaaccaa gtttggctta atagcatcct
    11461 tcaaaatcaa agccaaggca ccctcaacct gcaaatcacc tacagaaaca ggcgtacggt
    11521 catagcgata accaataacg atattcgcca aacgacgttt caagtcctcg atgtccgttg
    11581 ccaagcaaag aattgccatg atttctgaag caactgtaat atcaaaaccg tcctcacgtg
    11641 gaataccgtt tagaggacca ccaagaccaa cagtcacatg gcggagcgca cggtcgttca
    11701 agtccacaac gcgtttccag aggatacgac gttgatcaat tcccagctca ttcccttggt
    11761 gcaagtggtt gtcaatcaag gcagaaaggg cattgttggc agttgtaata gcatgcatat
    11821 ctccagtaaa gtggaggttg atgtcttcca ttggcagaac ttgtgcatac ccaccaccag
    11881 cagcaccacc cttgatcccc atgactggac caagagacgg ttcgcggata gcaatcatgg
    11941 tttttttgcc aatcttgttc aaggcatccg caagaccaat ggtaagcgtc gactttcctt
    12001 cacctgcagg tgttgggttg atggcagtaa ccaagatcaa tttaccgact ggattgctct
    12061 caactgcacg aattttatca aagctgagtt tagccttgta ctttccgtac aactccaaat
    12121 cgtcgtaaga aataccaagt ttctctacaa catcaacaat tggcttcaac tcaatactct
    12181 gtgcgatttc aatatctgtt ttcattcaaa attcctctaa cctcttatat gataattcat
    12241 tatatcacaa aacaagattt ttaacatcct aaaactctct aaacgttcgt aaatatctct
    12301 gtttttaagg cttttagagt cctttcttaa attttatatg gctttatagt ttgaaactat
    12361 agtaaatctt cgtttttacc aaaaatttat cactttcatt ttacttaccg cttatttttg
    12421 tgtacaatag tgctatgaaa attttagtta catcgggcgg taccagtgaa gctatcgata
    12481 gcgtccgctc tatcactaac cattctacag gtcacttggg gaaaattatc acagagactt
    12541 tgctttctgc agggcatgaa gtttgtttaa ttacgacaaa acgagctctg aagccagagc
    12601 ctcatcctaa cctaagtatt cgagaaatta ccaataccaa ggaccttcta atagaaatgc
    12661 aagaacgtgt tcaggattat caggtcttga tccactcaat ggctgtttct gactacactc
    12721 ctgtttatat gacagggctt gaggaagttc aggctagctc caatctaaaa gaatttttaa
    12781 gcaagcagaa tcatcaggcc aagatttctt caactgatga ggttcaggtt ttgttcctta
    12841 aaaagacacc caaaatcata tccctagtca aggaatggaa tcctactatt catctgattg
    12901 gtttcaaact gctggttgat gttaccgaag atcatctggt tgacattgca cgaaaaagtc
    12961 ttatcaagaa tcaagcagat ttaatcatcg cgaatgacct gactcaaatt tcagcaggtc
    13021 agcaccgagc tatatttgtt gagaaaaatc agcttcaaac agtccagact aaagaagaaa
    13081 ttgcagaact cctccttgaa aaaattcaag cctatcattc ttagaaagga aaactatggc
    13141 aaacattctc ttggctgtaa cgggttcaat cgcctcttat aagtcggcag atttagtcag
    13201 ttctctaaaa aaacaaggcc atcaagtcac tgtcttaatg actcaggctg ctacagagtt
    13261 tatccaacct ttgacactac aggtactctc acagaatcct gtccacttgg atgtcatgaa
    13321 ggaaccctat cctgatcagg tcaatcatat cgaacttgga aaaaaagcag atttatttat
    13381 cgtggtacct gcaactgcta acactattgc aaaactagct cacggatttg cggacaacat
    13441 ggtaaccagt acagctctag ccctaccaag tcatattccc aaactaatag ctcctgctat
    13501 gaatacaaaa atgtatgacc atccagtaac tcagaataat ctgaaaacat tagaaaccta
    13561 cggctatcag ctgattgctc ctaaggaatc tctactagct tgtggagacc acggacgagg
    13621 agctttagct gacctcacaa ttattttaga aagaataaag gaaactatcg atgaaaaaac
    13681 gctctaatat tgcacccatt gctatctttt ttgctaccat gctcgtgata cactttctga
    13741 gctcacttat ctttaacctt tttccatttc caatcaaacc gaccattgtt catattcctg
    13801 tcattattgc cagcattatt tatggtccac gagttggggt tacacttgga tttttgatgg
    13861 gattacttag cttgacggtt aacacgatta cgattctacc gacaagctac ctcttctctc
    13921 ccttcgtacc aaacggaaac atctactcag ctatcattgc catcgtccca cgtattttga
    13981 ttggtttaac tccttactta gtctataaac tgatgaaaaa caagactggt ctgattttag
    14041 ctggagccct tggttccttg acaaatacta tctttgtcct tggaggaatc ttcttcctat
    14101 ttggaaatgt ttataatgga aatatccaac ttcttctggc aaccgttatc tcaacaaatt
    14161 caattgctga attggtcatt tctgcaattc taaccctagc cattgttcca cgactacaaa
    14221 ccttgaaaaa ataaaaacaa tctccgcttt caaacctgaa ggtggagatt ttgctctata
    14281 cagtttcttt ttaatgaaat ctttactata gtaaaa
//
LOCUS       DBERDK010000046        13518 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.046, whole genome shotgun sequence.
ACCESSION   DBERDK010000046 DBERDK010000000
VERSION     DBERDK010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 13518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13518
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.046"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            101..970
                     /locus_tag="ACL73L_001422"
     CDS             101..970
                     /locus_tag="ACL73L_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HGS0772384.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLATAILGVVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLGPISLTNVQLMILAISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAALGGFVIGLLETFATAFGMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            974..1930
                     /locus_tag="ACL73L_001423"
     CDS             974..1930
                     /locus_tag="ACL73L_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HGS0772385.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAAAIIGSTSPTYGAFFGAMLVGALLSG
                     VVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVAS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            1930..2694
                     /locus_tag="ACL73L_001424"
     CDS             1930..2694
                     /locus_tag="ACL73L_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0772386.1"
                     /translation="MALLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKAKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLEYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            2694..3404
                     /locus_tag="ACL73L_001425"
     CDS             2694..3404
                     /locus_tag="ACL73L_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0772387.1"
                     /translation="MSMLKVENLSVHYGMIQAVRDVSFEVNEGEVVSLIGANGAGKTT
                     ILRTLSGLVRPSSGKIEFLGQEIQKIPAQKIVAGGLSQVPEGRHVFPGLTVMENLEMG
                     AFLKKNREENQANLKKVFSRFPRLEERKNQDAATLSGGEQQMLAMGRALMSTPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAISDRGYVLETGKIVLSG
                     TGKELASSEEVRKAYLGG"
     gene            3712..4368
                     /locus_tag="ACL73L_001426"
     CDS             3712..4368
                     /locus_tag="ACL73L_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HGS0772388.1"
                     /translation="MAVKDFMTRKVVYISPDTTVSHAADLMREQELHRLPVIENDQLV
                     GLVTEGTIAQASPSKATSLSIYEMNYLLNKTKVKDVMIRDVVTVSGYASLEDATYLML
                     KNKIGILPVVDNHQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIISL
                     IVEENLNISHTVNIPRKDGKVIIEVQIDGSIDLPALKEKFEANGIQVEEIARTSAKVL
                     "
     gene            4476..5571
                     /gene="prfB"
                     /locus_tag="ACL73L_001427"
     CDS             join(4476..4547,4549..5571)
                     /gene="prfB"
                     /locus_tag="ACL73L_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HGS0772389.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTELD
                     FWNDNIAAQKTSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQEKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSFEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            5589..6281
                     /gene="ftsE"
                     /locus_tag="ACL73L_001428"
     CDS             5589..6281
                     /gene="ftsE"
                     /locus_tag="ACL73L_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HGS0772390.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDVPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKRRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            6274..7200
                     /gene="ftsX"
                     /locus_tag="ACL73L_001429"
     CDS             6274..7200
                     /gene="ftsX"
                     /locus_tag="ACL73L_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HGS0772391.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYHKVYDSLKNMSTVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVEANTPNDVKTIAEDAKKIEGV
                     SEVQDGGANTEKLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMISPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLKI"
     gene            7486..9666
                     /locus_tag="ACL73L_001430"
     CDS             7486..9666
                     /locus_tag="ACL73L_001430"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HGS0772392.1"
                     /translation="MMKDTFKNVLSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVM
                     INPIFAPLVITGGILEQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINR
                     ITGTIFGVSGDMLKNPDAMVTTFFGGSIKVADYFISVLEAPALNMGVFVGIISGFVGA
                     TAYNKYYNFRKLPDALSFFNGKRFVPFVVILRSAIAAILLAAFWPVVQTGINNFGIWI
                     ANSQETAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYDILTGAAKGTQVFG
                     QDPLWLAWVTDLVNLKGTDASQYQHLLDTVHPARFKVGQMIGSFGILMGVIVAIYRNV
                     DADKKHKYKGMMIATALATFLTGVTEPIEYMFMFIATPMYLVYSLVQGAAFAMADVVN
                     LRMHSFGSIEFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPG
                     RNGNYETAEGSEETSSEVKVAAGSQAVNIINLLGGRVNIVDVDACMTRLRVTVKDADK
                     VGNAEQWKAEGAMGLVMKGQGVQAIYGPKADILKSDIQDILDSGEIIPETLPSQMTEA
                     QQNTVHFKDLTEEVYSVADGQVVALEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGT
                     VSSIFPTKHAFGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVTAGDLLVTADL
                     DAIRAAGRETSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
     gene            9718..10533
                     /locus_tag="ACL73L_001431"
     CDS             9718..10533
                     /locus_tag="ACL73L_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HGS0772393.1"
                     /translation="MKFLTLNTHSWMEKEAEEKFQILLEDILEKDYDLICFQEINQEM
                     TSSEVEVNDLYQALPAAEPIHQDHYVRLLVEKLLEQGKNYYWTWAYNHIGYDRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVALAETVVDGKELAVASVHLSWWDKGFQEE
                     WARFEAVLKKLNKPLLLAGDFNNPAGQEGYQAILASPLGLQDAFEVAQEKSGSYTVPP
                     EIDGWKGNTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAILNWK"
     gene            10673..12016
                     /locus_tag="ACL73L_001432"
     CDS             10673..12016
                     /locus_tag="ACL73L_001432"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGS0772394.1"
                     /translation="MSFTKFQFKNYIREALKELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVARQISAHSDVEVRV
                     VNYVGGTDKARQIEKLASNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLETVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDKNAQIYQLTQLMQPYLAMIFVNTKTRADELHSYLTAQGLKVAKIHGDI
                     APRERKRIMNQVQNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLGIKFSPKMVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
     gene            12231..13421
                     /gene="metK"
                     /locus_tag="ACL73L_001433"
     CDS             12231..13421
                     /gene="metK"
                     /locus_tag="ACL73L_001433"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HGS0772395.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
                     AELRKSGEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVIDK
                     VIKEVIPSSYLDDKTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAADLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
BASE COUNT         4010 a         2403 c         3020 g         4085 t
ORIGIN      
        1 ggctagtttc ctagtttgct ctttgatttt cattgagtat aagaacctat caaaaagtga
       61 gggaaacccc tcggaattat aaatagaaag agtgaatctt atgctacaac aactcgtaaa
      121 tggtttgatt ctaggtagtg tttacgcgct gttagcccta ggatatacca tggtttacgg
      181 aattatcaag ctcatcaact tcgcccatgg tgatatttat atgatgggag cctttatcgg
      241 ttatttcttg atcaattctt tccaaatgaa tttctttgta gcgcttattg tagctatgct
      301 agcgacagct attcttggtg tcgtgattga gtttcttgct taccgacctt tgcgccactc
      361 tactcgtatt gctgttttga ttacggctat tggggtttct ttcctattgg agtatggaat
      421 ggtctatctg gttggtgcca atacccgtgc cttccctcaa gcgattcaaa cagttcgata
      481 tgatttggga ccaattagct taacaaatgt gcagttaatg attttggcca tttccttgat
      541 tttgatgatt ttgttacaag tcattgtcca aaagactaag atggggaaag ccatgcgtgc
      601 agtatcagta gatagcgacg cggcgcaatt gatggggatc aatgtaaacc gtacgattag
      661 ctttaccttc gctttgggtt ctgctcttgc gggtgcggct ggtgttctga ttgctcttta
      721 ttataactct cttgagcctt tgatgggggt tactccaggt cttaaatctt tcgttgccgc
      781 agtacttggt ggtatcggaa ttattcctgg tgcggctctt ggtggctttg tgattggtct
      841 attggaaacc tttgcgactg cctttgggat gtcagatttc cgtgatgcca ttgtttatgg
      901 aatcttgttg ttgatcttga ttgtccgccc agctggtatc cttggtaaga atgtgaaaga
      961 gaaggtgtaa acgatgaagg aaaatttaaa agttaatatt ctatggttac tccttttgtt
     1021 agctggctat agcttgatta gtgtactggt ttcagtcgga gtacttaatc tattctatgt
     1081 acagatttta caacaaattg gaattaatat tattttggct gttggtctca acttaatcgt
     1141 tggtttttca ggacaatttt cacttggtca tgctggtttc atggcgattg gtgcctatgc
     1201 agcagctatt attggttcta catcaccaac ctacggtgcc ttctttggag ctatgcttgt
     1261 aggggctttg ctttcaggag tagttgcctt acttgtcggc attccaacct tgcgcttgaa
     1321 gggggactat cttgcggtag caactctggg tgtttctgaa attatccgta tctttatcat
     1381 caatggtgga agtcttacaa atggtgcggc aggtatctta gggattccta actttacaac
     1441 ttggcaaatg gtttacttct ttgtcgtgat tacaaccatt gcaaccttga acttcttgcg
     1501 tagcccaatt ggccgttcaa ccctctctgt tcgtgaagat gaaatcgctg ctgagtcagt
     1561 tggggttaat acgactaaaa ttaaaatcat cgcttttgtc tttggtgcca ttactgcaag
     1621 tattgctggg tcacttcagg caggatttat cgggtctgtt gtaccgaaag attacacctt
     1681 catcaactca atcaacgttt tgattattgt tgtatttggt ggactcggtt ccattacagg
     1741 tgcgattgtt tcggctattg ttctgggaat tttgaatatg cttctccaag atgttgctag
     1801 tgtgcgtatg attatttacg ctttggcctt ggtattggta atgattttca gaccaggtgg
     1861 actccttgga acgtgggaac tgagcctatc acgtttcttt aaaaaatcta agaaggagga
     1921 acaaaactaa tggcattact tgaagtaaaa cagttaacca aacattttgg tggtctaaca
     1981 gctgttggag atgtgactct tgaattgaac gaaggggaac tggttggatt aatcggtcca
     2041 aacggagctg ggaaaaccac ccttttcaac cttttgaccg gtgtttatga accaagcgag
     2101 ggaacagtaa ccctagatgg tcaccttttg aatgggaaat caccttataa gattgcctct
     2161 ttgggacttg gacgtacttt ccaaaatatc cgtcttttta aagatttaac agttttagac
     2221 aatgttttga ttgcttttgg aaaccatcac aaacagcatg tttttactag tttcttacgc
     2281 ttaccagctt tttacaagag tgaaaaagaa ttaaaggcta aagctttgga attgttgaaa
     2341 atctttgatt tagatggtga tgcagagact cttgctaaaa atctttccta cggacaacaa
     2401 cgtcgtttgg aaattgttcg tgcccttgct acggaaccta aaattctctt cttagatgaa
     2461 ccagcagcag gtatgaaccc acaggaaaca gccgaattga ctgagttaat tcgtcgtatc
     2521 aaagatgaat ttaagattac aatcatgttg attgaacacg atatgaatct ggtcatggaa
     2581 gtaacagaac gtatctacgt acttgaatat ggccgtttaa ttgctcaagg aactccagac
     2641 gaaattaaga ccaataaacg cgttatcgaa gcttatctag gaggtgaagc ctaatgtcta
     2701 tgttaaaagt tgaaaatctt tctgtgcatt acggtatgat ccaagcagtc cgtgatgtaa
     2761 gctttgaagt taatgaagga gaagttgttt cccttatcgg tgccaacggt gcaggtaaga
     2821 caactattct tcgcaccttg tcaggtttgg ttcgaccaag ttcaggaaag attgaatttt
     2881 taggtcaaga aatccaaaaa ataccagctc agaaaatcgt ggcaggtggt ctttcacaag
     2941 ttccagaagg acgccacgtc tttcctggct tgactgttat ggaaaatctt gaaatgggag
     3001 ctttcttaaa gaaaaatcgt gaagaaaatc aagctaactt gaagaaggtt ttctcacgct
     3061 ttcctcgtct tgaagaacgg aagaaccaag atgcagctac tctttcagga ggggaacaac
     3121 aaatgcttgc catgggacgc gctcttatgt caacaccaaa acttcttctt ttagatgaac
     3181 catcaatggg acttgcccca atcttcatcc aagagatttt tgatatcatt caagatattc
     3241 agaagcaagg aacaaccgtc ctcttgattg aacaaaatgc caataaagca cttgcaatct
     3301 ctgaccgagg atatgtactg gaaacaggga aaatcgtcct atcaggaaca ggaaaagaac
     3361 tcgcttcatc agaagaagtc agaaaagcat atctaggtgg ctaaaacaat ccagtggatt
     3421 gttttagtcg gcagatggag attacgaagt aatcatcaat atagtccggg ggaccttttt
     3481 agtcggtaga ttgagattgc aaacaaatct gcatctacat tgaaagctta atttctaata
     3541 attgaaaaaa tcgaatgaaa agtttcttac cttcattcac agagctcgat tttagagctc
     3601 tttttgctag cttattcata cttttctaaa ttttgaaaaa gaaatgtaag cgtttgatag
     3661 atttgcaaaa agattgtata atagggataa gaatagaaaa ggagaagtct catggcagtt
     3721 aaagatttta tgacccgcaa ggtagtttat attagtccag atacaacagt atctcatgca
     3781 gcagatttga tgagagagca agaattgcac cgcttgcctg ttatcgaaaa tgatcaatta
     3841 gttggtttgg tgactgaggg aaccattgca caagcaagtc catctaaagc aacaagtctt
     3901 tctatctatg agatgaatta tcttctgaat aagacaaaag taaaagatgt catgattcgc
     3961 gatgttgtca ctgtctcagg ctatgctagt ctagaagatg caacttatct gatgttgaaa
     4021 aataagattg gtattctccc tgtcgtagat aaccatcaag tatacggagt tattactgac
     4081 cgtgacgttt tccaagcctt tcttgaaatt gcaggttatg gcgaagaagg gattcgtgta
     4141 cgctttgtta cagaagatga agttggtgtt cttggaaaaa ttatttcttt gattgtagaa
     4201 gaaaatttga atatatccca tacagtcaat attccgcgta aggatggtaa ggtcattatc
     4261 gaagtgcaaa tcgatggatc aattgattta ccagccttga aagaaaaatt tgaagcaaat
     4321 ggtattcaag tggaagaaat cgctcgcact tcagcaaaag tcttgtaaga agggaagccc
     4381 aaaggcttct tttttcatga aaaggggatt agagcaaaag atggaaagaa atgataaaat
     4441 atgctataat gaaataatgt aaaaaaggag tatttatgga catttcagta attcgtcaga
     4501 aaattgacgc aaatcgtgaa aaattagctt ctttcagggg gtctctttga cctcgaaggg
     4561 ctagaggaag agattgccat cttggaaaac aagatgacag aacttgattt ttggaacgat
     4621 aatattgcgg cccaaaaaac gtcgcaagaa ttgaatgaat taaaaaacac ttacaatacc
     4681 ttccataaga tggaagagtt gcaggatgaa gtcgaaattt tattggattt tttggctgaa
     4741 gacgagtcag tgcatgatga actggtagcg cagttagccg aacttgataa gataatgacc
     4801 agctacgaga tgactctact cttgtcagaa ccttatgacc acaacaatgc catcttggaa
     4861 atccatccag gttctggtgg tactgaggcg caggactggg gtgatatgtt gcttcgtatg
     4921 tatactcgtt atggtaatgc taaaggcttt aaagtggaag tgttggatta ccaagcaggt
     4981 gatgaggctg gtattaagtc ggtaacttta tcatttgaag ggcctaatgc ctatggtctc
     5041 ctcaagtcag aaatgggtgt tcaccgctta gtgcgaatct caccatttga ctctgccaaa
     5101 cgtcgccata cctctttcac atctgtagaa gtgatgccag aattggatga tactattgaa
     5161 gtggaaatcc gtgaagatga tatcaagatg gataccttcc gttcaggtgg tgccggtgga
     5221 caaaacgtca ataaggtttc aacaggtgta cgtttaactc acattccaac tggaattgtt
     5281 gtccaatcaa cagtggatcg tacccagtat ggaaatagag atcgtgccat gaagatgttg
     5341 caggctaagc tctatcaaat ggagcaagag aagaaggctg cggaggtaga ttctctcaaa
     5401 ggtgagaaaa aggagatcac ttggggaagc caaatccgtt cttatgtctt cacgccttat
     5461 actatggtaa aagatcaccg aactagcttt gaggttgctc aggtagataa ggttatggat
     5521 ggggatctag atggttttat cgatgcttat ctcaagtggc gaattagcta agatagaaag
     5581 gaactcacat gtcaattatt gaaatgagag atgtcgttaa aaaatacgac aacggaacaa
     5641 ctgctctacg cggtgtttcg gtcagcgttc aaccggggga atttgcttac atcgtaggac
     5701 cttcaggagc agggaagtca acttttattc gttctctgta tcgtgaagta aaaatcgata
     5761 aaggaagcct atcagttgct ggttttaatc tggttaagat caaaaagaaa gatgtcccgc
     5821 ttctacgtcg tagtgttggg gttgtcttcc aggattataa attgttacca aagaaaactg
     5881 tctatgaaaa tattgcttac gctatggaag taatcgggga aaatcgccgt aatatcaaaa
     5941 gacgagtgat ggaagttttg gacttggttg gattgaagca taaggttcgt tctttcccaa
     6001 atgaactctc aggtggggag caacagcgga ttgcgattgc gcgtgcaatt gtaaataatc
     6061 ccaaagtatt gatagctgat gagccaacag gaaatctgga tccggataat tcatgggaaa
     6121 ttatgaatct cttggaacgg attaacctac aaggaacaac tattttgatg gcgactcata
     6181 atagccagat tgtaaatacc ttgcgccacc gtgtcattgc cattgaaaat ggccgtgtcg
     6241 ttcgtgacga atcaaaagga gagtatggat acgatgatta gtagattttt tcgccattta
     6301 tttgaagcct taaaaagttt gaaacgaaat ggttggatga cagtagctgc tgtcagttca
     6361 gtcatgatta ctttgacctt ggtggcaata tttgcatctg ttattttcaa tacagcgaaa
     6421 ctagctacag atattgaaaa taatgtccgt gtagtagttt atatccgaaa ggatgtggaa
     6481 gataatagtc agacaattga aaaagaaggt caaactgtta caaataatga ctaccacaag
     6541 gtatatgatt ctttgaagaa catgtctacg gttaaaagtg ttaccttttc aagtaaagaa
     6601 gaacaatatg aaaaattaac cgagataatg ggagataact ggaaaatctt tgaaggagat
     6661 gccaatcctc tctatgatgc ctatattgta gaggcaaaca ctccaaatga tgtaaaaact
     6721 atagccgaag atgctaaaaa aattgaaggt gtctctgagg ttcaagatgg cggtgccaat
     6781 acagaaaaac tcttcaagtt agcttcattt atccgtgttt ggggactagg gattgctgct
     6841 ttgttaattt ttatcgcagt tttcttgatt tcaaatacca ttcgtattac cattatttcc
     6901 cgcagtcgcg aaattcaaat catgcgcttg gtcggagcta aaaacagtta tatccgtgga
     6961 ccgttcttgt tagaaggagc ctttatcggt ttattgggag ctatcgcacc atctgttttg
     7021 gtctttattg tttatcaaat tgtttaccaa tctgtcaaca aatcgttggt agggcaaaat
     7081 ctatccatga ttagtccaga tttatttagt ccgttgatga ttgccctact atttgtgatt
     7141 ggggttttca ttggttcatt gggatcagga atatccatgc gccgattctt gaagatttag
     7201 gtaaaatagc tgcttttatg aggagattgt aaaatctcct tttttgctac aagagttttt
     7261 gaaaagagat gcgcagaaga aaagagcttc caaagaagtc ccccagagaa gacttcttga
     7321 tggctaattt gaacttgaaa tttggcgcac tagaaatttt ttagaaaaaa gtgtaatttt
     7381 tttataaaag cctttacaaa aaaaattaaa gtggtataat taaatcatag aaagtgcaaa
     7441 cgttttcgta aaacgtttgc ctaaataaaa ataaaggaga tttgaatgat gaaagataca
     7501 ttcaaaaatg tcttgtcttt cgaattttgg caaaaattcg gtaaggcttt gatggtagtt
     7561 atcgcggtta tgccggctgc tggtttgatg atttcaatcg gtaagtctat cgtgatgatt
     7621 aacccaatct ttgcaccact tgtcatcaca ggtggaattc ttgagcaaat cggttggggg
     7681 gttatcggta accttcacat tttgtttgcc ctagccattg gaggaagctg ggctaaagaa
     7741 cgtgctggtg gtgctttcgc cgctggtctt gccttcatct tgattaaccg tatcactggt
     7801 acaatttttg gtgtatcagg cgatatgttg aaaaatccag atgctatggt aactactttc
     7861 tttggtggtt caatcaaagt tgctgattac tttatcagtg ttcttgaagc tccagccttg
     7921 aacatggggg tattcgtagg gattatctca ggttttgtag gggcaactgc ttacaacaaa
     7981 tactacaact tccgtaaact tcctgatgca ctttcattct tcaacgggaa acgtttcgta
     8041 ccatttgtag ttattcttcg ttcggcaatc gctgcaattc tacttgctgc tttctggcca
     8101 gtagttcaaa caggtatcaa taacttcggt atctggattg ccaactcaca agaaactgct
     8161 ccaattcttg caccattctt gtatggtact ttggaacgtt tgctcttgcc atttggtctt
     8221 caccacatgt tgactatccc aatgaactac acagctcttg gtggtactta tgacatttta
     8281 actggtgcag ctaaaggtac tcaagtattc ggtcaagacc cactatggct tgcatgggta
     8341 acagaccttg taaaccttaa aggtactgat gctagtcaat accaacactt gttagataca
     8401 gtacatccag ctcgtttcaa agttggacaa atgatcggtt cattcggtat cttgatgggt
     8461 gtgattgttg ctatctaccg taatgttgat gctgacaaga aacataaata caaaggtatg
     8521 atgattgcaa cagctcttgc aacattcttg acaggggtta ctgaaccaat cgaatacatg
     8581 ttcatgttca tcgcaacacc tatgtatctt gtttactcac ttgttcaagg tgctgccttc
     8641 gctatggctg acgtcgtaaa cctacgtatg cactcattcg gttcaatcga gttcttgact
     8701 cgtacaccta ttgcaatcag tgctggtatt ggtatggata tcgttaactt cgtttgggta
     8761 actgttctct ttgctgtaat catgtacttt atcgcaaact tcatgattca aaaattcaac
     8821 tacgcaactc cagggcgcaa cggaaactac gaaactgctg aaggttcaga agaaaccagc
     8881 agtgaagtga aagttgcagc aggctctcaa gctgtaaaca ttatcaacct tcttggtgga
     8941 cgtgtaaaca tcgttgatgt tgacgcatgt atgactcgtc ttcgtgtaac tgttaaagat
     9001 gcagataaag taggaaatgc agagcaatgg aaagcagaag gagctatggg tcttgtcatg
     9061 aaaggacaag gggttcaagc tatctacggt ccaaaagctg acattttgaa atctgatatc
     9121 caagatatcc ttgattcagg tgaaatcatt cctgaaactc ttccaagcca aatgactgaa
     9181 gcacaacaaa acactgttca cttcaaagat cttactgagg aagtttactc agtagcagac
     9241 ggtcaagttg ttgctttgga acaagtaaag gatccagtat ttgctcaaaa aatgatgggt
     9301 gatggatttg cagtagaacc tgcaaatgga aacattgtat ctccagtttc aggtactgta
     9361 tcaagcatct tcccaacaaa acatgctttt ggtattgtga cggaagcagg tcttgaagta
     9421 ttggttcaca ttggtttgga cacagtaagt cttgaaggta aaccatttac agttcatgtt
     9481 gctgaaggac aaaaagttac agcaggagat ctccttgtca cagctgactt ggatgctatc
     9541 cgtgcagcag gacgtgaaac ttcaacagta gttgtcttca caaatggtga tgcaattaaa
     9601 tcagttaaat tagaaaaaac aggttctctt gcagctaaaa cagcagttgc taaagtagaa
     9661 ttgtaatata cttgaggttg gaagctgtat tccaacctct tattttggga gaaaagaatg
     9721 aaatttttaa cactcaatac tcacagttgg atggagaaag aagcagagga aaaattccag
     9781 attttgcttg aagatattct tgaaaaggac tatgatttga tttgtttcca agaaatcaat
     9841 caggagatga cctcgtcaga ggtggaggtt aatgaccttt atcaagcttt gccagcagct
     9901 gagcctattc accaagacca ttatgttaga ctcttggttg aaaagttgtt agagcaaggg
     9961 aaaaattact actggacctg ggcttataac catatcggct atgaccgcta ccatgaaggt
    10021 gtggctatct tgtctaaaac acctattgaa gccagagaaa ttttggtttc agatgtggat
    10081 gatccaacag actatcatac tcgccgtgtt gccctagctg aaactgtagt cgatggcaag
    10141 gagctagcag ttgccagtgt tcatctctct tggtgggata aaggtttcca agaagaatgg
    10201 gcacgatttg aggctgtctt gaaaaaattg aacaagccac ttttactagc tggagatttt
    10261 aacaacccag caggtcagga aggttaccaa gctattttag ctagtccatt aggcttacaa
    10321 gacgcatttg aagttgctca agagaaaagt ggtagctata ctgttccgcc tgaaattgat
    10381 ggctggaaag ggaacactga accccttcga atcgattatg tctttactac caaagagtta
    10441 gcggtggaaa atttacatgt cgtatttgat ggtaacaaga gtccacaagt gagtgatcac
    10501 tatggcttga atgctatatt aaactggaaa taataactga aaagaggttg gaactataaa
    10561 attccagcct tttcttacta gagaagctac tggaaatagc ctaaataagt gagactactg
    10621 taatggaata aaatatggta taattgataa ggtagataga atcgaggatg ttatgtcatt
    10681 tacgaaattt caatttaaaa actatattag agaagccttg aaggagttaa aatttacaac
    10741 tccaacagag gtgcaagaca agttgattcc tattgttttg gcaggtcgtg acctagtagg
    10801 agaatcaaaa acaggttcag gtaagactca tactttcttg ttaccgattt tccagcaatt
    10861 agatgaagct agcgatagtg tacaagcagt gattactgca ccgagtcgtg agttggctac
    10921 tcaaatttac caagtagcgc gtcagatttc agctcactca gatgtcgaag ttcgtgtggt
    10981 taattatgtg ggtggtacgg ataaggctcg ccagattgag aaattggcaa gcaatcagcc
    11041 tcatattgtt attggaacac caggccgtat ctacgacttg gttaaatctg gtgatttagc
    11101 tattcataaa gccaagacat ttgttgttga tgaagcagat atgaccttgg atatgggatt
    11161 cttggaaact gttgataaga ttgctggcag tcttccaaaa gacttgcaat tcatggtctt
    11221 ctcagcgact atcccacaaa aactgcaacc attcttgaaa aaatacttat caaatcctgt
    11281 tatggagaaa attaagacca aaacggttat ttctgacacc attgataatt ggttgatttc
    11341 gaccaaggga catgataaga atgctcaaat ttaccagttg actcagttga tgcagccgta
    11401 tttggcaatg atttttgtta acactaaaac gcgtgctgat gaattgcatt catatctgac
    11461 tgctcaaggc ttgaaggttg caaaaatcca tggcgatatt gcccctcgtg aacgcaagcg
    11521 aatcatgaat caggtgcaaa atctggattt tgagtatatt gtcgcaacag atttggcagc
    11581 gcgtgggatt gacattgaag gtgtcagcca tgtcatcaat gatgccattc cgcaagactt
    11641 atcttttttt gttcatcgtg ttggtcgtac tggacgaaat ggcctaccag gtacagctat
    11701 taccctttat cagccaagtg atgactcgga tatccgtgag ttggagaaat tgggaatcaa
    11761 gtttagtcct aagatggtca aagacgggga atttcaagat acctatgacc gtgatcgtcg
    11821 tgccaaccgt gagaaaaaac aagataaact tgatatcgaa atgattggtt tggttaaaaa
    11881 gaaaaagaaa aaagtcaaac cgggttataa gaagaaaatt caatgggcgg ttgatgaaaa
    11941 gcgccgtaaa accaagcgtg ctgaaaatcg cgctcgcggt cgtgcagagc gtaaagctaa
    12001 acgccaaaca ttttaataga aattgttgga gtattgagct ccaacttttt gctttatgag
    12061 aacgaactat ctaaaccgaa acactacatt aaagactgca aattgcgatt aaaaatggta
    12121 taatgataaa gttatatagt cccgataaga tggtaggtat ttattacgaa gagttttcct
    12181 atcagtactt tgtaactcta taacaatatt ttttaagggg ggacattttt atgtcagagc
    12241 gtaaattatt cacgtctgaa tctgtatctg aggggcatcc ggataagatt gcagaccaaa
    12301 tttcagatgc gattttggat gctattttag caaaggatcc agaggcgcac gttgctgctg
    12361 aaacagctgt atatactggt tctgtccacg tttttggtga aatttctaca aatgcctatg
    12421 tggatattaa ccgtgtggtt cgtgatacca ttgcagagat tggttatacc aatacagaat
    12481 atggattttc tgctgagacg gtgggagtac acccatcttt ggtggaacaa tctcctgaca
    12541 tcgctcaagg tgttaacgaa gccttggagg ttcgtggaaa tgctgatcaa gatccactgg
    12601 acttgattgg agcaggtgac caagggctca tgtttggatt tgcagtagat gaaacagaag
    12661 agcttatgcc attgccaatt gcactcagtc ataaattggt tcgtcgtctg gcagaacttc
    12721 gtaagtctgg agaaattagc tatctccgtc cagatgcaaa atcacaagtt acagttgagt
    12781 acgatgaaaa tgaccgtccg gtacgtgtag atacagtcgt tatttctact cagcatgatc
    12841 cagaggccac taatgaacaa atccatcaag atgtgattga caaggtcatc aaagaagtta
    12901 ttccatcttc ttatcttgat gataagacaa aattctttat caatccgaca ggtcgttttg
    12961 taatcggtgg tcctcaaggg gactcaggtt tgactggtcg taagattatt gtagatactt
    13021 atggtggcta ctctcgtcat ggtggtggtg ccttctctgg taaagatgcg actaaggtgg
    13081 atcgttcagc ctcttatgcg gctcgctata ttgccaagaa tatcgttgca gcagaccttg
    13141 ctaagaaggc agaagtgcag ttggcctatg ctatcggtgt tgcgcaacct gtttctgttc
    13201 gtatcgatac tttcggtaca ggaacagtag ctgaaagtca acttgaaaaa gcggctcgtc
    13261 aaatctttga ccttcgccct gcagggatta tccaaatgct ggacctcaag cgtccaattt
    13321 accgtcaaac atcggcttac ggtcacatgg gacgtacaga tattgatctt ccatgggaac
    13381 gtttggataa ggtagatgct ttgaaagaag cagtaaaata agattttaag aggggaacgt
    13441 cctctctttt ttatagtttt taactatact gggatactgt tctgaaaatc cattttgcga
    13501 aagtagagat ttacggct
//
LOCUS       DBERDK010000047        13403 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.047, whole genome shotgun sequence.
ACCESSION   DBERDK010000047 DBERDK010000000
VERSION     DBERDK010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 13403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13403
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.047"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            148..804
                     /locus_tag="ACL73L_001434"
     CDS             148..804
                     /locus_tag="ACL73L_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HGS0772396.1"
                     /translation="MPTALGYVSIGLACGTIGAPYVTPVEMGLMSLFVYAGSAQFAML
                     ALIVVQAPVAAIAMTVFLINLRLFLLSLHASTYFRHTSLWYNIGMSSILTDETYGVLM
                     GELAHTDKVNPMWMHGNNLNSYVAWFVGTVVGTALGGLLPNPEIFGLDFALVGMFIGI
                     FASQFQIMQRRIPVRNLLIILAVVAVSFFLLLTVVSQSLAVLFATLLGCSMGVVLDGQ
                     "
     gene            794..1117
                     /locus_tag="ACL73L_001435"
     CDS             794..1117
                     /locus_tag="ACL73L_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HGS0772397.1"
                     /translation="MVSKYLLLAVIFSGLVTWILRMIPFILVKYKGLPAIVERFLKFL
                     PVSIIFALILSSVVTGKVGSLPQIKWLDFLAVFPTAWVAFRYRNLVGTVLFGVVLIAI
                     LRLVF"
     gene            1221..2051
                     /locus_tag="ACL73L_001436"
     CDS             1221..2051
                     /locus_tag="ACL73L_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HGS0772398.1"
                     /translation="MKKIVKYSSLAALGLVAAGVLAACSGGAKKEGEAASKKEIIVAT
                     NGSPRPFIYEENGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYNMAV
                     NNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
                     EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAQGQDELKSFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            2205..3059
                     /locus_tag="ACL73L_001437"
     CDS             2205..3059
                     /locus_tag="ACL73L_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HGS0772399.1"
                     /translation="MKIKKWLGLVALATVAGLALAACGNSEKKADNAITIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKE
                     NGKDLVAIADTYISPIRLYSGLNGSANKYTKVEDIPANGEIAVPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKALFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEESS
                     DGLDQPVW"
     gene            3159..4532
                     /locus_tag="ACL73L_001438"
     CDS             3159..4532
                     /locus_tag="ACL73L_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HGS0772400.1"
                     /translation="MVFPSEQEQIEKFEKDHVAQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVW
                     TEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESA
                     STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEE
                     VSRIYGLELPLLQEERMAFLKRFFFEPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYYTHIELVE
                     ELIRSYE"
     gene            4525..5586
                     /locus_tag="ACL73L_001439"
     CDS             4525..5586
                     /locus_tag="ACL73L_001439"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HGS0772401.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTLVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSELSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILALLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEEGSVLEIFSNPKQPLTQDFISTATGIDEAMVKIEKQEIVEHLSENSL
                     LVQLKYAGASTDEPLLNELYKHYQVMANILYGNIEILDGTPVGELVVVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGVQ"
     gene            5588..6280
                     /locus_tag="ACL73L_001440"
     CDS             5588..6280
                     /locus_tag="ACL73L_001440"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HGS0772402.1"
                     /translation="MESLIQTYLPNVYKMGWAGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAVPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIIIILIIFAIQF
                     LGDFLTKKLSHK"
     gene            complement(6310..6879)
                     /locus_tag="ACL73L_001441"
     CDS             complement(6310..6879)
                     /locus_tag="ACL73L_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HGS0772403.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPRFGAITTIGLVIAFFFLGTKHGAGSFLPGIICGLLADEVAHLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVTPNPGTV
                     LLFIASIVIGALVGALIGQALSKKFAQKI"
     gene            7014..7628
                     /locus_tag="ACL73L_001442"
     CDS             7014..7628
                     /locus_tag="ACL73L_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HGS0772404.1"
                     /translation="MGRFLDFVFNRFFLGMIATAFFWLLTLAGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSLYKQNFVSSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATLLNVLVVALIFLAYTVSLKLQVYFALSYRNSLKLSLIGIFMSLAAVAKVLLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLEPVYEIIHEKLATK"
     gene            7606..9276
                     /locus_tag="ACL73L_001443"
     CDS             7606..9276
                     /locus_tag="ACL73L_001443"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGS0772405.1"
                     /translation="MKNWRQNRMKQFWLHTLLRTYSSVMIIIIASFAILLSYADWDSR
                     EKEAQRVAQRVTARTVSEIEYYHRESTQIAQALVENQARIEGIYKYFSLSMPDYFYWQ
                     LERKASPYISVSLYENVDDLYVRNDFVTGVAIAFQDYKEVYVSTKDKRSGEKIRAEDF
                     KPAGNSFAIPVSDPVSDQDLGVIYISLDPAVLYHAIDNTRGHTPMAVTVTSPFDTEIF
                     HMGETVDKESENWLVGLTSHGYQVQVAVPKNFVLQGTVTSSALIVGLSLLFIVILYLT
                     LRQTFANYQKQVVDLVDSIQAIAQGQEGLRIDTLEKDQELLLIAETTNDMLDRLEKNI
                     HDIYQLELSQKDANMRALQAQINPHFMYNTLEFLRMYAVMQSQDELADIIYEFSSLLR
                     NNISDERETLLKQELEFCRKYSYLCMVRYPKSIAYGFKIDPELENMKIPKFTLQPLVE
                     NYFAHGVDHRRTDNVISIKALKQDGFVEILVVDNGRGMSAEKLANIREKLSQKYFEHQ
                     ASYSDQRQSIGIVNVHERFVLYFGDRYAITIESAEQAGVQYRITIQDE"
     gene            9288..10574
                     /locus_tag="ACL73L_001444"
     CDS             9288..10574
                     /locus_tag="ACL73L_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HGS0772406.1"
                     /translation="MYKVLLVDDEYMVTEGLKRLIPFDKWDMEVVATASHADEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKEILPDAAYILLSGYQEFDYVKRAMNLSVVDY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDEAGFVSYLGDKENWWIGLSKEK
                     QGSFTIPYYVLGQDWQIFISDQPLDGLVVTPFEAPYQEHFERWKLNAEKTLFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLLEKVVLENTPRVPITKQLFIQFVMDVF
                     HLFEHLKADDLTDIVKTIHAIQSFDELVPYIKETLSSFFGQYRMNENVVSVLEVIGRD
                     YQKELSLKDISKALFINPVYLGQLIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     ICYAVGYSNLGYFYKVFRKLCGKSPKAYRKQVETIL"
     gene            10626..11159
                     /locus_tag="ACL73L_001445"
     CDS             10626..11159
                     /locus_tag="ACL73L_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS0772407.1"
                     /translation="MKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRAAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDESMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKALAEEEETEELT"
     gene            11237..11707
                     /gene="nrdI"
                     /locus_tag="ACL73L_001446"
     CDS             11237..11707
                     /gene="nrdI"
                     /locus_tag="ACL73L_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HGS0772408.1"
                     /translation="MKTISLVYISLSGNTESFVTRLKDYLLSQYKGIEVQKIHIKDLV
                     KEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEKEN"
     gene            complement(12000..13262)
                     /locus_tag="ACL73L_001447"
     CDS             complement(12000..13262)
                     /locus_tag="ACL73L_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NRAMP family divalent metal transporter"
                     /protein_id="HGS0772409.1"
                     /translation="MSQAISLNQSTWASKLKAMGPGILMATAAVGGSHIVSSTQAGGS
                     YGWSLLLLVILANVFKYPFFRFGAEYTADTGKTLVEGYAEKGKLYLWIFFILNVFSAM
                     VNTAGVAILCSAIIASAFPMIGLSITQWSLILVAIIWAMLLFGGYKLLDGMVKWIMSA
                     LTIATVLAVIIAAVKHPEYSSDFVEKTPWQMAALPFIVSLLGWMPAPIEISAINSLWS
                     AEKRKTVNFNTEDALFDFNTGYIGTAILAVFFVALGALIQYPTGQAVEAASAKYISQF
                     VGMYASVLGEWSRYLITFIAFLCIFGTVITVIDGYSRVNQESLRLLISQKEDNRKSLN
                     IWMTITAIIGIVIIKFFAGQVSTMLRFAMIGSFLTTPFFALLNYALVTRENKNLPSWL
                     KHLAIAGLIFLFGFAIFFIYALAIGKAG"
BASE COUNT         3924 a         2513 c         3084 g         3882 t
ORIGIN      
        1 tcagttctat ctgcaacctc aaaacagtgt tttgagcaac ctgcggctag tttcctagtt
       61 tgctctttga ttttcattga gtattattaa gacaatatga ggagaaagat gaaagaaaaa
      121 ggattttagg agggggcgca ggcagccatg ccaacggctc ttggctatgt cagtatcggc
      181 ctggcctgtg ggactatcgg tgcgccctat gtgacacctg ttgagatggg cttgatgagt
      241 ctctttgttt atgctgggag tgcccagttt gccatgttgg cactgattgt ggttcaagct
      301 cctgtggcag ctattgctat gacggttttt ctaatcaact tgcgtctctt tttgttgagt
      361 ttacacgcat cgacttattt ccgtcatacc agtctttggt acaatatcgg tatgtctagt
      421 atcttgacag atgagaccta tggcgttttg atgggtgaat tggcccatac agacaaggta
      481 aatcctatgt ggatgcacgg aaacaatctt aacagctatg tggcttggtt tgtggggaca
      541 gtagtcggaa cggctctggg tggcctgcta ccaaatccag aaatctttgg cttggatttt
      601 gccctggttg ggatgtttat tggtattttt gcttcgcaat ttcagattat gcaaagacgg
      661 attcctgtcc gcaatctgct cattatccta gcagttgttg cggtgtcctt ctttttgctc
      721 ttgacagtgg tgtctcagtc actagctgtt ctgtttgcga cgctacttgg ttgtagcatg
      781 ggggtggttt tagatggtca gtaagtatct tttattagca gttattttct ctggcttggt
      841 gacttggatt ctccgtatga ttcccttcat cttagtcaag tataagggct tgcctgcaat
      901 cgttgagcgt tttttgaagt tcttgcccgt ttccattatc tttgccttga ttctttcaag
      961 cgtagtgaca ggtaaggttg ggagccttcc tcaaattaaa tggctagact tcttagccgt
     1021 ctttccaaca gcttgggtag cctttcgcta ccgcaatcta gtcggaacag ttctctttgg
     1081 agtggtcttg attgccatct tgcgtttggt cttttaaatt acccataaaa aaaacttatc
     1141 acagagatag atatcatata atggcgtaaa tgctcttttt ctgttaagat tataaggtat
     1201 tctattttgg aggaaatgac atgaaaaaaa tcgttaaata ctcatctctt gctgccctag
     1261 gacttgttgc tgcaggtgtg cttgcggctt gctcaggggg tgctaagaaa gaaggagaag
     1321 cagctagcaa gaaagaaatc atcgttgcaa ccaatggatc accaaggcca tttatctatg
     1381 aagaaaatgg cgaattgact ggttacgaga ttgaagtcgt tcgcgctatc tttaaagatt
     1441 ctgacaaata tgatgtcaag tttgaaaaga cagaatggtc aggtgtcttt gctggtcttg
     1501 acgctgaccg ttacaatatg gctgtcaaca atcttagcta cactaaagaa cgtgcggaga
     1561 aatacctcta tgccgcacca attgcccaaa atcctaatgt ccttgtcgtg aagaaagatg
     1621 actctagtat caagtctctc gatgatatcg gtggaaaatc gacggaagtc gttcaagcca
     1681 ctacatcagc taagcagtta gaagcataca acgctgaaca cacggacaac ccaactatcc
     1741 ttaactatac taaggcagat ttccaacaaa tcatggtacg tttgagcgat ggacaatttg
     1801 actataagat ttttgataaa ataggtgttg aaacagttat caagaaccaa ggtttggaca
     1861 acttgaaagt tatcgaactt ccaagcgacc aacaaccgta cgtttaccca cttcttgctc
     1921 aaggtcaaga tgagttgaaa tcatttgtag acaaacgcat taaagaactc tacaaagacg
     1981 gaacgcttga aaaattgtct aaacaattct tcggagatac ttatctacca gcagaggctg
     2041 atattaaata atttcttgaa atcatccatt ctgagtaaga tgggtgattt ctcagttttt
     2101 agggatatag ttttaaacta tatatctgat tatctaataa caatttgtga aatcaaacaa
     2161 attgtgagat actagtacgg tattattttt aaggagaaag aatcatgaaa atcaaaaaat
     2221 ggcttggcct agtagccctt gctacagtcg caggtttggc tcttgcagct tgcggaaact
     2281 cagaaaagaa agcagacaat gcaataacta tcaaaatcgc aactgttaac cgtagcggtt
     2341 ctgaagaaaa acgttgggac aaaatccaag aattggttaa aaaagacgga attaccttgg
     2401 aatttacaga gttcacagac tactcacaac caaacaaagc aactgctgat ggcgaagtag
     2461 atttgaacgc tttccaacac tataacttct tgaacaactg gaacaaagaa aacggaaaag
     2521 accttgtagc gattgcagat acttacatct ctccaatccg cctttactca ggtttgaatg
     2581 gaagtgccaa caagtacact aaagtagaag acatcccagc aaacggagaa atcgctgtac
     2641 cgaatgacgc tacaaacgaa agccgtgcgc tttacttgct tcaatcagct ggcttgatta
     2701 aattggatgt ttctggaact gctcttgcaa cagttgccaa catcaaagaa aatccaaaga
     2761 acttgaaaat cactgaattg gacgctagcc aaacagctcg ttcattgtca tcagttgacg
     2821 ctgccgttgt aaacaatacc ttcgttacag aagcaaaatt ggactacaag aaagcacttt
     2881 tcaaagaaca agctgatgaa aactcaaaac aatggtacaa catcattgtt gcgaaaaaag
     2941 attgggaaac atcacctaag gctgatgcta tcaagaaagt aatcgcagct taccacacag
     3001 atgacgtgaa aaaagttatc gaagaatcat cagacggttt ggatcaacca gtttggtaat
     3061 aagaaacagg gaggtgggag agagaattcc acctcttgct tttgtataga gcacggagtg
     3121 taaagaagac tattcgttca tagaaaggta gagagaatat ggtttttcct agcgaacaag
     3181 aacagattga aaaatttgaa aaggatcatg tagcccagca ttattttgag gttttgcgta
     3241 ccttgatttc taagaaatca gtctttgccc agcaggttgg actcaaggaa gtcgcaaatt
     3301 atctgggtga gattttcaag cgtgttggag ctgaagtgga gattgatgag agctatacag
     3361 cgccctttgt catggcacat ttcaagagtt cgcgtccaga tgccaagacc ttgattttct
     3421 ataaccacta tgacactgtg ccagcggatg gggatcaggt ctggacagag gatcctttta
     3481 cgctttcggt ccgcaatggc ttcatgtatg ggcgtggagt tgatgacgac aagggtcata
     3541 tcacagctcg cttgagtgct ttgagaaaat atatgcagca ccatgatgat ttacctgtca
     3601 atatcagctt tatcatggag ggagcggagg aatcggcttc aacagaccta gataagtatt
     3661 tggaaaagca tgcagacaaa ctccgtgggg cggatttgtt ggtctgggaa caagggacca
     3721 aaaatgcctt ggaacagctg gaaatttctg gtggcaataa ggggattgtg acctttgatg
     3781 ccaaggtaaa aagcgctgat gtggatatcc actcgagtta tggtggtgtt gtggaatcag
     3841 ctccttggta tctcctccaa gccttacagt ctcttcgtgc tgcggatggc cgtatcttgg
     3901 ttgaaggctt gtacgaagaa gtacaagagc ccaatgaacg agaaatggcc ttgctagaaa
     3961 cttatggtca acgaaaccca gaggaagtta gtcggattta tggattggag ttgcctctct
     4021 tacaggagga gcggatggcc tttctaaaac gtttcttttt cgagccagcg cttaatatcg
     4081 aaggaatcca gtctggttat caaggtcagg gtgttaagac tattttacct gcagaagcca
     4141 gtgccaagct agaggttcgt ctggttccgg gcctagaacc gcatgatgtt ctggaaaaaa
     4201 ttcggaaaca gctagacaaa aatggctttg ataaggtaga attatactat accttgggag
     4261 agatgagcta tcgaagcgat atgagcgcac cagccattct caatgtgatc gagttggcca
     4321 agaaattcta tccacagggc gtttcagtct tgccgacgac agcggggaca ggacctatgc
     4381 atacggtctt tgatgcccta gaggtaccaa tggttgcatt cggtctagga aatgccaata
     4441 gccgagacca cggtggagat gaaaatgtgc gaatcgctga ttattacacc catatcgaat
     4501 tagtagagga gctgattaga agctatgagt agagatatta tcaagttaga tcagatcgat
     4561 gtgacttttc accaaaagaa gagaaccatt acagcggtta aggatgtgac cattcacatc
     4621 caagaagggg atatctacgg aatcgttgga tattctggag cagggaaatc aacccttgta
     4681 cgggtgatta acctcttgca aaaaccatct gcagggaaaa ttaccattga cgacgatgtg
     4741 atttttgacg gcaaggtgac cttgacggca gagcagttgc gtcgtaaacg tcaagatatc
     4801 ggaatgattt tccagcattt taacctgatg agccaaaaga cagcagagga gaatgtagcc
     4861 tttgccctta aacactctga actcagcaag gaagaaaaga aggctaaagt agctaagttg
     4921 ttggacttgg ttggtttggc agatcgtgct gaaaactacc cttcacaact atctggaggg
     4981 caaaaacagc gtgtggcaat tgcgcgtgcc ttggccaatg atccaaaaat cttgatttca
     5041 gacgagtcaa cttctgccct tgatccgaag acaaccaagc agattttggc cttgttgcaa
     5101 gatttgaacc aaaaattagg cttgactgtt gtcttgatta cgcatgaaat gcagattgtc
     5161 aaagacattg ccaaccgtgt tgcagttatg caggatgggc atttgattga agagggtagt
     5221 gtgcttgaaa tcttctcaaa ccctaaacaa cctttgactc aagactttat ctcaacagct
     5281 acaggtattg acgaagccat ggtcaaaatc gagaagcaag aaatcgtgga acacttgtct
     5341 gaaaacagtc tcttggtgca actcaagtac gctggagctt caacagacga gccacttttg
     5401 aatgaattgt acaagcatta ccaagtaatg gctaatattc tctatgggaa tatcgaaatt
     5461 ctcgatggta ctcctgttgg agaattggtg gtggttttgt caggtgaaaa agcagcgttg
     5521 gcaggtgccc aagaagccat tcgtcaagca ggtgtacaac taaaagtatt gaagggagta
     5581 cagtaagatg gaatcattga ttcaaaccta tttaccaaat gtctataaga tgggttgggc
     5641 tggtcaggca ggctggggaa cggctatcta cttaactctt tatatgacag ttctttcctt
     5701 cattatcgga ggcttcttgg ggctagtggc aggtctcttt ctcgtcttga cagcgccagg
     5761 tggtgtcttg gagaataaag tcgtattctg gattttagac aaaattacct caatttttcg
     5821 tgcggttccc tttatcatcc tcttggcaat cttgtcacca ctttctcact tgattgttaa
     5881 aacaagtatc gggccaaatg cagcccttgt cccactttct tttgcagtct ttgccttctt
     5941 tgcccgtcag gtgcaggttg tcttggctga attggatggc ggtgtcattg aggcggctca
     6001 agcgagcgga gcgactttct gggacatcgt gggtgtttac ctatcagaag gtcttccaga
     6061 tttgatccgt gtgacgactg tgaccttgat ttcccttgtt ggggaaacag ctatggccgg
     6121 tgcggttgga gctggtggta tcggtaacgt agccatcgct tatggattta accgctacaa
     6181 tcacgatgtg accatcttgg caaccatcat tatcattttg attatctttg caatccaatt
     6241 cttgggagat ttcttgacta agaaattgag ccataaataa aaaagagccg tgtggctctt
     6301 tttaactgat cagattttct gggcaaattt tttactcaag gcttgtccaa tcaaggcacc
     6361 cactagggct ccgatgacaa tacttgcgat aaatagaagg acagttccag ggtttggagt
     6421 gaccatgata cggtcgatat attcttggga ttttcctctt gccagaagag tagccatata
     6481 ggctttgggc gcaatccaca taagcaagat tggtcctgtt gtactaaagg cgaaaataat
     6541 gaaagaaagg aagttctttg ttttgtcctt gtattttcct aaatgagcta cttcatctgc
     6601 taggaggcca cagataattc caggaaggaa ggaaccagca ccgtgtttag ttcccaagaa
     6661 aaagaaggca atgacaaggc cgatagtggt aatggctcca aagcgcggaa cttttgcgac
     6721 taggatcata tagacgctac cgccgacaag ggcagtaaag gcaggcgcat aaaacatgtt
     6781 tccagtttgg tcaaagagat tgcccaaaag gacaccaatc cccatgcaga ggaagtaaag
     6841 aactgcaaaa agcagtgtag ttaagataga ttttttcatg aattgtctcc tgataatttt
     6901 ttcacaattc tcattgtacc acggtttggt gggattgtaa atggatacta gaagtttttg
     6961 aaaacgcttg aaatatgata taatggtttc atagttattt ttaggggaat atcatgggta
     7021 gatttttaga ctttgtcttt aatcgtttct ttttagggat gattgcgaca gccttctttt
     7081 ggctattaac tttagcagga gggattatcc ttggtctagc gccggctagt gccaccttga
     7141 tgagcttata tgcagaacat ggttatagct ttcgggaata cagtttgaag gaggcttggt
     7201 ctctttacaa gcaaaatttt gtctcaagca acctgatttt ctatagcttt ttaggtgtgg
     7261 gtctagtttt gacctatggt ttgtatctct tggtgcaatt gcctcatcag accattgttc
     7321 atttgattgc gacccttttg aatgtcctag tagttgccct gatctttttg gcttatacag
     7381 tatctttaaa attacaagtt tattttgcct tgtcctatcg aaatagtctc aaattatcct
     7441 tgattggcat ctttatgagt ctagcagctg tggccaaggt tctccttggg actgtgctac
     7501 ttgtagcaat tggttactat atgcctgccc tgctattttt tgtaggaatt gggatgtggc
     7561 atttctttat cagtgatatg ttggaacctg tctatgaaat catccatgaa aaattggcga
     7621 caaaatagaa tgaagcagtt ttggctacat acgcttctaa gaacctatag ttcagtgatg
     7681 atcattatca ttgcgagttt tgcaatctta ctctcttacg ctgactggga ttcacgtgaa
     7741 aaggaagccc agagagtagc ccagcgtgta actgctagaa cagtgagtga aattgaatat
     7801 taccatagag agtcaaccca gatagctcag gctttagttg aaaaccaagc tcgtattgag
     7861 ggaatctata aatactttag ccttagcatg ccagactatt tttactggca attagagcgg
     7921 aaagcttcgc cttatatatc agtctctctg tatgaaaatg ttgatgacct ctatgttcga
     7981 aatgattttg taactggggt ggccattgct tttcaagatt acaaggaagt ctatgtttct
     8041 actaaagaca aacgtagtgg agaaaaaatc agggctgagg atttcaaacc agcaggaaat
     8101 agttttgcca ttccagtgtc agatccagtg tcagatcaag acttaggagt gatttacatc
     8161 tccttggatc ctgctgtttt ataccatgcc attgataata ctagaggtca tactccgatg
     8221 gcagtaacag tgacctcacc ttttgatacg gagatttttc atatgggtga gacagttgat
     8281 aaggagagtg aaaattggct agttggctta acttctcatg gttatcaggt tcaggtggca
     8341 gttcccaaaa actttgtttt acaaggaacg gtgaccagct ctgctttgat tgtgggcttg
     8401 agccttctct ttattgtcat tctttatctg actttgaggc agacctttgc taattatcaa
     8461 aagcaggtag tggatttggt ggattccatc caagctattg cccaaggaca agaaggtctt
     8521 cgcattgata cgcttgaaaa ggatcaggaa ttgctcctaa tcgcggagac gaccaatgat
     8581 atgttggatc gattggaaaa gaatatccat gatatttacc agttagaact cagtcaaaaa
     8641 gatgccaata tgcgggcctt gcaggcgcaa atcaaccctc acttcatgta taatacgctg
     8701 gagttcttgc gtatgtacgc agtcatgcag agtcaagatg agttggcaga tatcatttat
     8761 gaattcagta gtctcttgcg taacaatatt tccgacgaaa gagagaccct cctcaaacag
     8821 gaattagaat tttgccgtaa atacagctat ctctgcatgg ttcgctatcc caagtccatt
     8881 gcctatggtt tcaagataga tccagagtta gagaatatga agattcccaa gtttaccttg
     8941 caaccgctgg tagaaaacta tttcgcgcat ggtgttgacc acaggcggac agataatgtg
     9001 attagcatca aggctcttaa acaggatggg tttgtggaaa ttttggtggt cgataatggt
     9061 agaggaatgt cggctgaaaa gttggcaaat atccgagaaa aattaagtca gaaatatttt
     9121 gaacaccaag ccagctacag tgatcaaagg cagtctatcg ggattgtcaa tgtacacgag
     9181 cgttttgtgc tctattttgg agaccgctat gccattacta tagagtctgc agagcaagcc
     9241 ggtgttcagt atcgtattac aattcaagat gagtagaaag ggagaaaatg tataaagtat
     9301 tattagtaga tgatgagtac atggtgacag aaggtctgaa gcgtttgatt ccctttgata
     9361 agtgggatat ggaggtcgtc gcaacagcca gtcatgccga tgaagctcta gaatatgttc
     9421 aggaaaatcc tgtcgatgtc atcatttccg atgtcaatat gccagacaaa acagggcttg
     9481 atatgattcg ggagatgaaa gagatcttac cagatgctgc ctatatcctg ctctcaggtt
     9541 atcaggagtt tgattatgta aaaagagcaa tgaaccttag tgtggtggac tatttggtca
     9601 agcctgttga taaggtagag ctgggaaatc tgctggagaa gattgcaggt cagctcggcg
     9661 agagagggaa gaaaagtcag actcttagtc aagaattaga cgaggctgga tttgttagtt
     9721 atttagggga taaggagaat tggtggatag gtctatccaa ggaaaaacaa ggttccttca
     9781 ccattcccta ctatgtcttg ggccaagact ggcagatttt catttctgac caacccctag
     9841 atggtttagt cgttacacct tttgaagctc cttatcaaga acactttgaa cgctggaagc
     9901 tgaatgctga gaaaaccctc ttttacggtt ctgtaaattt gcagcagtct gagagtctct
     9961 ttgcctatta cgaaccgatt tatagggtta tcattcaggg aaatctcaat caaatcgtag
    10021 aagagttaaa tctcttggag aaggtagttc ttgaaaatac gccgcgtgtt ccgattacta
    10081 aacagctttt tattcagttt gtcatggatg ttttccattt atttgaacat ctcaaagctg
    10141 atgacctgac agacatcgtc aaaaccattc atgctattca atccttcgat gaattggttc
    10201 cttatatcaa ggaaactctg agcagctttt tcggtcaata ccgtatgaat gaaaatgtgg
    10261 tcagtgtgct ggaagtcatt ggtcgtgatt accaaaaaga gctttccctc aaggatatca
    10321 gtaaggctct ctttatcaat cctgtctatc tagggcagtt gattaagcgt gaaaccgatt
    10381 cgacttttgc agagttacta aacaaacaac gtattaaggc tgcccaacaa cttttgcttt
    10441 caactagtga cagcatcgaa gatatttgtt atgctgttgg ttacagtaac cttggatatt
    10501 tctataaagt tttccgaaaa ttgtgcggaa aatcgccaaa agcctaccga aaacaggtag
    10561 aaactatact ataagatttg tattccttta caaaaggtgc tataatatta tcaggaggtc
    10621 ctatcatgaa aaagaaacca atttatctat gggtcttgct aatcttgtct gcccttattt
    10681 cagttccgtc tctgtttgga attgtgagtc ccttgcctag taaagaagcc cttcgtgctg
    10741 ctcagaaaca agttgcaggg gtcaatgctc agcaattaga agaccagctt aattatacct
    10801 atagagtagc agaagcatct cattctattt ttaatgttgc tttgattgtg ctatctacta
    10861 ttttagtggt cgtagcgatt gtcttccttg ttcgtaaaaa tttgcaatat gcaaactata
    10921 cttacgttgg ctatgttttg ttagctatta ttggttcgat ttatggctat gttggtttac
    10981 aagacgctgt gcagttggtt caagatgaga gcatgcgttt gacagtgagt attggctcaa
    11041 aagctgttag cattttctat atcgtcatca atgttctctt cctagccctt gtcttctata
    11101 agatgtggcg acaacagaaa gccttggcag aagaagagga aacagaagag cttacctaag
    11161 tattgacaaa aaagaactat caagatacaa tcttggtagt tgttttttga ttatagataa
    11221 aatcatggag gtacatatga agacaatttc tttagtttat attagtttga gtggcaacac
    11281 tgaaagtttt gtgacgcgct tgaaagacta tctcttgtcc cagtacaaag ggattgaggt
    11341 tcaaaagatt catatcaagg atttggtcaa ggaaggcaaa aatttctatg aaatggacca
    11401 tccctatgtc gcttttttgc cgacctatct cgaaggtggg aatggcgtgg ataacggaga
    11461 tgttgagatt ttgacgacac cggtgggaga ttttattgcc tatggtaaca atgctagtaa
    11521 gtgttttggt gtggttgggt caggaaatcg taactttaat aaccaatact gcctaacagc
    11581 caagcaatac agtcaacgct ttggcttccc tgtattggct gactttgaaa tgcgaggcat
    11641 gctggaagat atcaaacatg tcgcagctat tatcgcagat ttgtatgagt tggaaaagga
    11701 gaattaatac tcaatgaaaa tcaaagagca aactaggaag ctagtcgcag gttgctcaaa
    11761 gcacagcttt gaggttgcag atagagctga cgtggtttga agagattctc gaatagtata
    11821 aagttcttat aagaggtggt tgaagttgtt caactgtttt ttgagtataa acagtcttta
    11881 agaacgtaaa aaagcataag ttcaggtctt caaaaccttg ttcttatgct ttttatcata
    11941 gaaatatttg ctgaattttc tcttgaaatg aaaccttatc ttcatctcgc gcttgtccct
    12001 taccctgctt ttccgattgc gagtgcgtag ataaagaaga tggcgaagcc aaagaggaaa
    12061 atcaatcccg caatggcaag gtgtttgagc caagaaggaa gatttttgtt ttcacgcgtt
    12121 accaaggcgt aattcaaaag agcaaagaaa ggtgttgtca ggaaagatcc aatcatggca
    12181 aagcggagca tggttgaaac ctgaccagcg aagaacttga taatgacgat accgatgata
    12241 gcagtgatgg tcatccagat gttcaaagat ttacgattgt cctctttttg actgattagc
    12301 agtcggagag attcctgatt aacgcgagaa tagccatcga taacagttat aactgttcca
    12361 aagatacaga ggaaggcaat aaaggtaatc aagtaacggg accattcgcc aagaacagag
    12421 gcatacatgc ccacgaattg agagatgtat ttggctgaag cagcttcaac cgcctgccct
    12481 gtaggatact gaatcagtgc tcccagtgcc acaaagaaga cggctaggat agctgttcca
    12541 atataaccag tgttaaagtc aaacagagcg tcttctgtgt taaagttgac ggtctttctc
    12601 ttttcagctg accaaagtga attgatggct gaaatttcaa taggagccgg catccatcct
    12661 aggagggaga cgatgaaggg cagagctgcc atttgccaag gtgtcttctc gacaaaatca
    12721 gaactgtatt ctggatgctt gaccgccgca atgataactg caagaacagt cgcaatggtt
    12781 aaggcagaca taatccattt gaccatgccg tctaaaagtt tgtagcctcc aaagagtagc
    12841 atagcccaaa tgattgcaac gagaatgagg gaccactgag taatgctaag tccaatcatt
    12901 gggaaggcac tggcgatgat agctgagcac agaatggcaa caccagccgt gttgaccata
    12961 gccgaaaaga cattgagaat aaagaaaatc cagagataga gttttccttt ttcggcataa
    13021 ccttcaacca aagtctttcc agtatcagct gtgtattcag caccaaaacg gaaaaatgga
    13081 tatttaaaga cattggctaa gatgaccaag agaagtagag accaaccgta agaaccgcca
    13141 gcttgagttg aggatacaat gtgggaacct ccaacagcgg cagttgccat taggattcca
    13201 ggtcccattg cttttagctt gcttgcccag gttgattgat ttaaggaaat agcttgcgac
    13261 atgataaata ctcctttttg aattttcaga ataatctgta tatgcaatct atattctaca
    13321 aaaaattttg gaaaaatgca agaatatttt gaaaaaagat agaaaattta caaagaaaat
    13381 ctgaaataac tgttatgata tag
//
LOCUS       DBERDK010000048        13339 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.048, whole genome shotgun sequence.
ACCESSION   DBERDK010000048 DBERDK010000000
VERSION     DBERDK010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 13339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13339
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.048"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            153..500
                     /locus_tag="ACL73L_001448"
     CDS             153..500
                     /locus_tag="ACL73L_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HGS0772410.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     AGTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFIHITDGQINVRCDS
                     SMSAQEIKDFAGL"
     gene            complement(779..1615)
                     /locus_tag="ACL73L_001449"
     CDS             complement(779..1615)
                     /locus_tag="ACL73L_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HGS0772411.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADF
                     EANPELLKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGARKEDTTLVKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKE
                     GQ"
     gene            complement(1628..2257)
                     /locus_tag="ACL73L_001450"
     CDS             complement(1628..2257)
                     /locus_tag="ACL73L_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGS0772412.1"
                     /translation="MLELRNINKVFGDKQILSNFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLSVLENLTLSPVKT
                     MGMKQEEAEKKASGLLEQLGLGGHAEAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            complement(2267..2908)
                     /locus_tag="ACL73L_001451"
     CDS             complement(2267..2908)
                     /locus_tag="ACL73L_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGS0772413.1"
                     /translation="MSYLFEILPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLVPMFLAGAIYLILIGIVTIISKKVEKKYSYYR"
     gene            complement(3054..4283)
                     /locus_tag="ACL73L_001452"
     CDS             complement(3054..4283)
                     /locus_tag="ACL73L_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HGS0772414.1"
                     /translation="MNNSQQMLQALEEQDLTKAEHYFAKALENDSSNLLYELATYLEG
                     IGFYPQAKEIYLKIVEDFPEVHLNLAAIASEDGQIEEAFTYLEEIQADSDWYVSSLVL
                     KADLYQLEGLTDVAREKLLEALTYSEDSLLILGLAELDSELENYQAAIQAYAQLDNRS
                     IYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELASLYFDQEEYQK
                     ATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALRIAKQGLEKNPFETRLLLAASQ
                     FSYELHDASGAENYLLTAKEDAEDTEEILLRLATIYLEQERYEDILDLQSEEPENLLT
                     KWMIARSYQEMDDLDTAYEHYQELTGDLKDNPEFLEHYIYLLRELGHFEEAKVHAHTY
                     LKLVPDDVQMQELFERL"
     gene            complement(4273..5439)
                     /locus_tag="ACL73L_001453"
     CDS             complement(4273..5439)
                     /locus_tag="ACL73L_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HGS0772415.1"
                     /translation="MEQKEKHFSLSWFFKWFLDNKAITVFLVTLLLGLNLFILSKISF
                     LFSPVLDFLAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVFVIIALFIIWG
                     LAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQAT
                     VLASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNYLTQFIPRKLK
                     EPVGQVLSDVNQQLSNYVRGQVTVAIIVAVMFIIFFKIIGLRYAVTLGVTAGILNLVP
                     YLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPINVL
                     FVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFEWYKVVSGLYELEGEEVKSEQ"
     gene            5583..6596
                     /locus_tag="ACL73L_001454"
     CDS             5583..6596
                     /locus_tag="ACL73L_001454"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HGS0772416.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEEGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHSGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VITYDLDQEGKLSKLYTYHSKPGAGSRHIIFHNHYKIAYLICELNNTIEVLIYDGVGE
                     FERMQVISTLPEAYEGFNGTAAIHLSKDGKYLYASNRGHDSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPDQKFLIVVHQDSDNATVFKRNCDNGRLAELSNDFHVPEAVCIR
                     FAP"
     gene            complement(6641..7060)
                     /locus_tag="ACL73L_001455"
     CDS             complement(6641..7060)
                     /locus_tag="ACL73L_001455"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HGS0772417.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDDKDHVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AERAIEEAQDKHLIDQERRAKIALQRAINRINVGNRL"
     gene            complement(7071..8477)
                     /gene="atpD"
                     /locus_tag="ACL73L_001456"
     CDS             complement(7071..8477)
                     /gene="atpD"
                     /locus_tag="ACL73L_001456"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HGS0772418.1"
                     /translation="MSSGKIAQVIGPVVDVLFAAGEKLPEINNALVVYKNDERKTKIV
                     LEVALELGDGMVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKTLVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMGF"
     gene            complement(8563..9441)
                     /locus_tag="ACL73L_001457"
     CDS             complement(8563..9441)
                     /locus_tag="ACL73L_001457"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HGS0772419.1"
                     /translation="MAVSLNDIKTKIASTKNTSQITNAMQMVSAAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLADQPSFDQVRKIISKTV
                     EMYQNELFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            complement(9457..10962)
                     /gene="atpA"
                     /locus_tag="ACL73L_001458"
     CDS             complement(9457..10962)
                     /gene="atpA"
                     /locus_tag="ACL73L_001458"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HGS0772420.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENVMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYAGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALTHGFLDTVPV
                     DDIVRFEEEFHTFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            complement(10977..11513)
                     /locus_tag="ACL73L_001459"
     CDS             complement(10977..11513)
                     /locus_tag="ACL73L_001459"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HGS0772421.1"
                     /translation="MDKKTVKVIEKYSMPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDVLVDCLNRLE
                     KETNRFEVTITSAHPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            complement(11513..12007)
                     /gene="atpF"
                     /locus_tag="ACL73L_001460"
     CDS             complement(11513..12007)
                     /gene="atpF"
                     /locus_tag="ACL73L_001460"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HGS0772422.1"
                     /translation="MHVTVGELIGNFILITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKALIDQYIDQ
                     LGEA"
     gene            complement(12021..12737)
                     /gene="atpB"
                     /locus_tag="ACL73L_001461"
     CDS             complement(12021..12737)
                     /gene="atpB"
                     /locus_tag="ACL73L_001461"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HGS0772423.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNVLEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKLQTI
                     DGTNWWSSPTANLQYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQAIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            complement(12772..12972)
                     /locus_tag="ACL73L_001462"
     CDS             complement(12772..12972)
                     /locus_tag="ACL73L_001462"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HGS0772424.1"
                     /translation="MNLTFLGLCIACMGVSVGEGLLMNGLFKSVARQPDMLSEFRSLM
                     FLGVAFIEGTFFVTLVFSFIIK"
     gene            complement(13047..>13339)
                     /locus_tag="ACL73L_001463"
     CDS             complement(13047..>13339)
                     /locus_tag="ACL73L_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HGS0772425.1"
                     /translation="EFNRLFDVFSEEHIYYAHPYASWERGTNENHNRLIRRWLPKGTK
                     KMTPKEVAFIEKWINNYPKKCLDYKSPREDFWMANLNLKFSKMEIIFIKRFQ"
BASE COUNT         4105 a         2956 c         2373 g         3905 t
ORIGIN      
        1 gctccataat atctataagg gatttaccca ctacaaatat tatagagcct aaaagatgag
       61 accaaccaac ctcatctttt ttcgtatcaa atctaagcaa ttttagaaac tttatggcat
      121 actagactta tcaatgaaag cgaggtaatc tcatggaaca atttttagat aacatcaaag
      181 accttgaagt cactacagtt gtgcgtgcgc aagaagctct tgataaaaaa gaaactgcaa
      241 ccttctttat cggtcgcaaa acttgccctt actgccgtaa atttgcaggt acattgtcag
      301 gtgtcgtagc tgaaaccaaa gctcacattt acttcatcaa tagtgaagaa ccaagccaac
      361 tcaatgattt gcaagcattc cgctcacgct atggaatccc aactgtacca ggttttattc
      421 atattacaga tggacaaatc aacgtccgtt gcgactcttc aatgtcagca caagaaatca
      481 aagatttcgc aggattgtaa aaaatcagct aacaagtctt aaaatcacaa aaacgcataa
      541 tgtaaggtgc aaaaaccttg atattatgcg ttttattgtt cctaaaacag ttacatcagc
      601 atttcattac agaacatttt aattttggaa gtcaaatcaa tttttaacaa tattttagaa
      661 atcacagtgg gctattccat attcaattat ccagattcaa tcgctattat cgttttccta
      721 gattacaaaa aaaactacgt tttgctaatc aaaacgtagt ctcagccact attttatctt
      781 actgtccttc ttttacttct ttggttgcta catcttctcc aaaccatttt tggctgattt
      841 cttggaactt gccgtccttg taaagactag aaaaagcttc atttatcttc ttaaccaagg
      901 ttgtatcttc cttacgggct ccaaccgcaa aagcttctgt ttctagtcca actgtaaaga
      961 cattataatc gtttaaaaca ccttctgctt ctaaataata gtttgcatag acacggtcaa
     1021 tcaatagacc atcaattcga tcgtttttca aatcaatcaa ggcttcatta aaggtttggt
     1081 attgattcgc ttccttatta gcgacaatat tcttcaaaag ttctggattt gcttcaaagt
     1141 ccgcataacc agatgaacca gcttgagctc ctaatgtctt tccagtcata tcctttgcag
     1201 tcgtgatacc agatgatttc ttcgtaacca atacctgctc attcttcata tatgagttac
     1261 tgaaagccac cttttcacgg cgttcgtctg tagcggaata gccattccaa atcagatcaa
     1321 tcgttccttt tgtcaattca gcttctttca aatcccaatc aatcggttgc caatttaccg
     1381 tgattccgta tttttcaaaa acagctgtag ctaaatcaat atcaaatcct gcataagaac
     1441 catctttctg agcaaatccc attggaacaa aagtactatc aaatccaata gtaatagact
     1501 tgttagactg gtactttgac caattatctc cactagtttc gctagaattt ttcccacaag
     1561 ctactaagaa caaagcagtc atcagactga ctaatacaag catccatttt ttcatccatt
     1621 tttcctccta tttaggttct actttcaata atacatctgc gatattttca gcaaactgca
     1681 aatcatgggt aaccacaatc tgggtcatcc caagttccct attttgcaag attagcttct
     1741 ccacttccaa acgtaattct ggatccaggg cagaagttgg ttcatcgtag ccaatgattt
     1801 ctgggtcaat catcatagca cgcgccaaag ccactcgctg cttttgccca ccagatagtg
     1861 agaaaggata ggcctctgcg tgtcctccta gtcctaactg ttccaagagt ccactcgcct
     1921 tcttctcagc ctcttcctgc ttcattccca tggtcttcac aggcgataaa gtcaaatttt
     1981 ccagaactga tagatgagga aatagttgaa aatcttggaa gacaaatccc agtaggttgc
     2041 gcttctgcaa ttcatccagc tctaaaggtt gtccattata aaagatttgc cctgaatcaa
     2101 tggtttcaag acctgcaagc atacgtaaaa gagttgtctt acctccacca gaaggtccaa
     2161 cgatagccag gatttgcttt tcaggaatac ttagactgaa attagacagg atttgtttgt
     2221 ctccaaagac tttattgata tttcgtaatt ctaacatggc agcctcctat ctataataac
     2281 tatacttctt ctcaactttt ttggaaataa ttgtcacaat cccaatcaaa atcaaataaa
     2341 tggctcctgc caagaacata ggaactagac tagcatcgcg gttagcagct gttcgactag
     2401 ccaagataag gtctgaaatt ccgagagcat agaccaaaga agtatccttg accaaactca
     2461 taacttcatt aaagacacta ggaagaacaa tcttggtcac ttggggcaag ataatatagc
     2521 gcactctgtc aaaagggcta aactttaaga ccttggcagc ctcatactgt cctcttggaa
     2581 tagtgtcaat tcccccacgg aaaatttctg caaagtaagc tgcatagttg agaacaaagg
     2641 caataatagc tgcaggaagg cggtctaaac gaatcccaat acttgggagc acataataga
     2701 taaaaatcag ttgcaagagt aagggggttc ctcgcataac ccagatgtaa atgttaatca
     2761 gataatggag gggcttccaa tggacttgca aggcaaaggc aatcaaaacg cccaagggaa
     2821 tcgaaaatag caagaccagt gcaaagacct gtacagtcgt gctcgcacca ttcagtaaac
     2881 tcggtaatat ctcaaataaa taagacatac tgcacctcct aaaagtaatt gttcctatta
     2941 tagcatgaat aaacaagatt accaagtatt tttgtaaaaa gattctacca tatacaaata
     3001 aaaaccgtag ctatcaaaag agataaacta cagttctata tgtttaaaca ttcttacaat
     3061 ctctcaaaca gttcttgcat ttgcacatca tctggaacca gttttaagta agtgtgagca
     3121 tggacttttg cttcttcaaa atgtcccaat tcacgcaaga gatagatata gtgttccaga
     3181 aattctggat tgtccttcaa atctcctgtc aactcttgat aatgctcata agcagtatcc
     3241 aaatcgtcca tttcttgata agaacgagca atcatccact tggtcaaaag attttctggc
     3301 tcctcactct gcaagtctag aatatcctca taacgctcct gctccagata aatagtggct
     3361 aaacgaagca agatttcttc tgtatcctca gcgtcttctt ttgcagtaag gagataattt
     3421 tctgcaccac tagcatcatg caattcataa gaaaattgtg aagcagctag caagaggcga
     3481 gtttcaaagg gatttttctc taatccttgc ttagcgatac gcagggcttc ttgaacttga
     3541 tgttccttat gtaaagcctg actgtaccca tactcatagc cttcaaagtc aggagaaatg
     3601 gtatcaagct gcttaaagta gagggtggct ttttgatatt cttcttgatc aaaataaaga
     3661 ctggccaact caaaagctgt taagtcatcg tattctaact ccagggcttt ttctaaaaac
     3721 tcagtagccg tttcaaattt ccctaactga gcataggcaa agccaattcg ttgataggtg
     3781 gaaatgcccg tttgctcata aatcgagcga ttatctaact gggcataggc ttgaatagcc
     3841 gcttggtaat tttccaactc actatccaac tctgccaaac ccaatatcaa gagagaatcc
     3901 tctgagtagg tcaaggcctc caataatttc tcacgtgcca catctgtcaa accttccagc
     3961 tggtaaaggt ctgccttcaa aaccaaagac gagacatacc agtcactgtc agcttggatt
     4021 tcctcaagat aggtaaaggc ttcttctatt tgaccatcct cgctagcaat tgcagctaga
     4081 ttaagatgaa cctctggaaa atcctctaca attttcaggt aaatttcctt ggcctgagga
     4141 tagaaaccaa tcccttcaag ataagttgcc aactcataca gaagattact tgaatcattt
     4201 tctaaagctt tggcgaaata atgctcagcc ttagttaaat cttgctcctc caaagcctgt
     4261 aacatctgtt gactattgtt cactcttgac ttcctcaccc tctaattcat atagaccact
     4321 gactacctta taccattcga aaatggctga aatgacaacc ttagcagagg cataaaccgg
     4381 aataccaagt aaaactcccc agataccaaa catagatcct gaagttaaca aaacaaagag
     4441 aacattaata gggtggatgt ttaattgact tcccaaaatc aatggagaga caaaacggcc
     4501 ttcaatagtt tgttctacga taaagacaat cactactttc aaaagcatga ctggaccagc
     4561 aatcaaaccc aatactagag caggaagcat ggctagaaag ctaccaagat aagggaccag
     4621 atttaaaata ccagcagtaa cccccagcgt aaccgcatag cgtagaccaa taatcttgaa
     4681 gaagatgata aacattactg ctacaataat agccactgtc acttgccctc gaacatagtt
     4741 ggacaactgt tgattcacat ctgataaaac ttgtccaaca ggttccttca attttcttgg
     4801 aatgaattgg gtcaaatagt tacgcaagcc tttcccatca cgcaagagat aaaagagcat
     4861 gaaaggaacg ataatcaagg caacaatcac ttgagaagcc ccgctaataa aggcactcac
     4921 ccagttgact gcctgagatg aaaccttact tgccaaaact gtagcctggc tagaaaaatt
     4981 ggtcaaaact tgctctaatt gaggtctgaa atcatctggc aggtgctggg ctaccaatcc
     5041 attaacaatc ctatctatat cttctaagta aacaggaacg tttcttgcaa aggtcaaaac
     5101 ctgacgttgc agatttggaa tggcgactgc caagccccaa atgataaaga gagcgatgat
     5161 aacaaagaca atagtgatag ctataacacg attaacctta tgcttctcca tccaatcaac
     5221 aataggattc aacaaataat ataacaaacc agacaaaatg actggcaaca tcacaactgc
     5281 taaaaagtct aaaacaggtg aaaatagaaa actaatctta cttaaaataa aaagattcag
     5341 tcccaataat aaggttacta aaaataccgt aattgccttg ttatctaaaa accacttgaa
     5401 aaaccaagat aggctaaaat gtttctcttt ttgctccata aatacttcct ttctattgtt
     5461 ctctcattat accatttttg aaacaaaata actctttttt aaagtgctta catctaattg
     5521 gatatcttta atttacttgt ttttgtgata taatagtctt atctttacac agaggtatag
     5581 taatgaaaga aactgtttat tttggaactt atacacgtcg tacttctcaa gggatttaca
     5641 aggcagactt tgatacagaa actggtcagc tttcaaatct agaacttttt gcagctgagc
     5701 caagtccaac ctaccttgcc tttgaccagc accaacattt atacactgtt ggtagccaag
     5761 acgataaggg gggaattgca gcctatcaaa ctgacgggac tgtgttaaat catgttgttg
     5821 aagaaggagc tccccactgt tatgttgctg tcgatgaaaa gcgtgatttg gtttacgcag
     5881 ctaactatca caagggacaa gtccttgttt ataaacgcca ggaagatggt agtcttctac
     5941 ttagtgatat ggatcaacac agtggccaag gtccacatga aaatcaagct tccccccatg
     6001 ttcactatac agatttaaca cctgaccact atctagtgac ctgcgacttg ggaactgacc
     6061 aagtcatcac ctatgacctc gatcaagaag gaaaattatc taagctctat acctatcaca
     6121 gcaagccagg agcaggctca cgccatatca ttttccataa ccactataaa atcgcttatc
     6181 tcatttgtga actcaataat actatcgaag ttttaatcta cgatggcgtt ggcgaatttg
     6241 aacgtatgca ggtcatttca actttaccgg aagcttacga aggctttaat ggaaccgctg
     6301 ctattcatct ctctaaagac ggtaaatacc tctacgcttc taaccgtggc catgattcta
     6361 tcgcagtata taccatcctt gcggacggta gcttagagtt attagaaatc gttccaacgc
     6421 atggtcagac tccacgtgat tttgatttga cacccgacca aaaatttctc attgttgtcc
     6481 atcaagactc tgacaatgca actgtcttta aacgtaattg tgacaatggt cgtctagcag
     6541 aactctccaa cgacttccat gttcccgaag cagtctgcat ccgttttgct ccttaaaaca
     6601 ccataaaaaa tgaacttggt attttcaagt tcattttttc ttatagtcta tttccgacat
     6661 taatacggtt aatggcacgt tgcaaagcaa tcttagcacg acgttcttgg tcaatcaaat
     6721 gtttgtcttg tgcttcttca attgcacgtt ctgcacgaag tttggcacgt tctgcacgac
     6781 tgatatcgat atcacgagca cgttctgcag agtcagcgac gattgtgatc atatcattgg
     6841 caatttcaat aacgccaccg tttactgcaa tccagttcac gtgatcttta tcatcgatac
     6901 gttttacctt tacttcatca actgctaaaa ccgcaatcat attttcatgt cgtggcaaga
     6961 tccccatctc accatccaga gttcgaaccg atacatagct ggcatggtga tcatagacga
     7021 gaccatctgg tgtcacgatc tggacagtta actgagccat agatcacctc ttaaaatccc
     7081 attttttcag cttttgcaat cacatcttcg atagaaccta caccacggaa ggcatcttct
     7141 ggcaagtggt cgtatttacc atcaaggatt tccttaaagc cacgtacagt ttcagcaact
     7201 ggaacataag aacctggctg accagtaaat tgttccgcaa cgttgaagtt ttgtgacaag
     7261 aagaactgga tacgacgggc gcgagcaacc aaggtctttt cttcatcaga aagctcatcc
     7321 ataccaagga tagcaatgat atcttgcaat tcatggtaac gttgaaggac acgttttact
     7381 tcagcagcaa ctgcatagtg ctcttctcca acgatttcag gtgccaaggc acgtgagctt
     7441 gaagcaagtg ggtcaacggc tgggtagata cccaattgta ccaacttacg ttccaagttt
     7501 gttgttgaat ccaagtgagc gaaggctgtt gctggcgctg ggtcagtata gtcatccgct
     7561 ggcacataga tagcctggat agaggttaca gaacccttct tggttgatgt gatacgttct
     7621 tgcaattgac ccatttccgt agcaagtgtt ggttggtaac caacggctga tggcatacga
     7681 cccaaaaggg cagatacttc tgaaccagcc tgagtgaaac ggaagatatt atcgataaag
     7741 agaagcacgt cttggccttc cacatcacgg aagtattcag cgattgtcaa accagtaagg
     7801 gcaacacgca tacgtgctcc tggtggctca ttcatctgac caaagaccat ggctgttttc
     7861 tcgataacgc ctgattcttt catttcccag taaaggtcat tcccctcacg agtacgttcc
     7921 ccaacaccag taaatactga aataccaccg tgctcttggg caatgttgtg aatcaattct
     7981 tggattaaga cggttttacc aactccggca ccaccgaaaa gtccaacttt accaccttta
     8041 aggtaagggg caagaaggtc aataaccttg atccctgttt caaggatttc agaagaggta
     8101 gacaactcat caaaagttgg agctttttta tgaattggct gacgctctgc gtcttctgta
     8161 aaaggagctt ccaaatcaat ggtatctccc aaaacgttga agacacgtcc caaagtttct
     8221 ttacctactg gtacagagat tggacgacct gtgtccaata cttccattcc acgagtcaac
     8281 ccatctgttg attccatggc gatagtacga accataccat ctcctaactc caaggctact
     8341 tcaaggacga tttttgtttt tctttcgtca tttttgtaga cgacaagtgc attgttaatc
     8401 tcaggaagtt tttcccctgc tgcaaacaaa acgtctacaa cgggaccgat aacctgagca
     8461 attttacctg aactcatctc cttctcctat tctatataag atactgtcgg ttcctagctc
     8521 aactaggtcc taagttcatt ttcatacgag ctggactaga gcctattcta aggcactagc
     8581 acctgctacg atttctgtaa tttcttgtgt aatcgccgcc tgtctggcac ggttatactg
     8641 aattgtcaaa tcattgatga ctttcttagc attatctgtc gctgtttgca tggctgtcat
     8701 acccgcagca ttctcagctg tcttggcatc gataatggca ccgtaaatca tactttctgc
     8761 aaactgaggc aacaactgct ccagaatttc ttctcggctg gtttccaatt caaaagtcaa
     8821 gctgtactct tcatccgctt catttggatc caagtcaaca atcggaagca tttgttccac
     8881 acgcatttga ctggttagcg tattgacatg gtggttgtag caaacataaa gctcatcaaa
     8941 gagttcattt tggtacattt caacagtttt tgaaataatc ttacgaactt gatcaaagct
     9001 aggttggtct gccaagccac gtaattcata aagtggttga ataccgcgag ccttaaagaa
     9061 atcagctccc atcccaccga tacagatcat ttcaaaacct ttaccgtctg ggtggtattc
     9121 ttctttcaac tccataacag ctttcaaaat agaggaatta taacctccaa ccaaaccgcg
     9181 gtctgaagtg ataacgatat agcctgtctt cttcacagaa cggctaatca acatcggatt
     9241 agttgaagca ccagctccat taccatgaag gatatctgtc aaaagtttac gcactttctg
     9301 agcgtaaact tggaagttgc gagcagcttc ttcagaacga cctagcttag cagccgatac
     9361 catttgcatg gcattagtga tttgactcgt attttttgtt gaggcgattt ttgttttaat
     9421 atcatttaga gatactgcca tctgacacct ctattcttat tggaagctag attgattgag
     9481 aaactctgta atcgcagcat ccaagactgc ttcttctggc aagtcttttg tatcacgaat
     9541 ggtttccaaa atctctggat gttgagcatc aaagaaggta tggaactctt cctcgaaacg
     9601 aacaatatca tctactggaa cagtatccaa gaaaccatgt gtcaaagcat aaagaatggt
     9661 tacttgtttc tcaacaggta atggtttgtg aacaggttgt ttcaagacct caacggtacg
     9721 acgtccacgg ttcaacttag cctgtgttgc tgcgtccaag tcagaaccaa acttagtaaa
     9781 ggcttccaac tcacggtatg aagcaaggtc gatacgaagt gtaccagcaa ccttcttcat
     9841 ggctttgatt tgtgcagaac cacctacacg agatacagat gaacccgcat cgatggctgg
     9901 acgaatacct gcattgaaga ggccatcgcc aaggaagatt tgtccatcag tgatagaaat
     9961 cacgttggtt gcgatatagg ctgagatatc tcctgcttgt gtctcgataa atggtagggc
    10021 tgtaattgat ccaccaccaa gttcatcaga aactttagct gagcgctcaa gcaaacggct
    10081 gtggagatag aaaacatccc ctgggaaggc ttcacgacct ggaggacgac gaagcaagag
    10141 cgacagttca cgataagcta ccgcttgttt tgatagatca tcgtatacaa tcaaaacatg
    10201 cttaccttga tacataaatt cttccgccat agcaacccca gcataaggag ctaggaagag
    10261 caatggagat ggttgtgaag cagaggctgt cacaacgatt gtgtagtcca aggcaccgta
    10321 ctgacgaagt gtttctactt gcgtacgaac tgttgattct ttttgtccaa tcgcgacgta
    10381 gatacagatc atatcttgat ctttttggtt caagattgta tcaatcgcaa tggttgtttt
    10441 ccctgtctga cggtcaccga taatcaactc acgttgacca cgaccaatcg gtacaagggc
    10501 gtcaatagct ttcaaaccag tttgcaatgg ttctgaaaca gacttacgtt gcataacacc
    10561 aggagctggt gcttctactg gacgagtttt atcagtgtgg atttctccaa gaccgtcaac
    10621 tggacgacca agcggatcca caacacgacc aatcagactt tcacctacag ggacttccat
    10681 gattttccct gtacggcgga ttgtatcgcc ttcacggata tctgtaaagt cacctaggat
    10741 gataatacca acgtctgttg actccaagtt ttgagccata ccataagagc cgttttcaaa
    10801 attcaacaac tctccactca tgacattttc aaggccgtga gcacgcgcga taccgtcccc
    10861 gatataggtt acaacacctg tttcagtcac atcaaaattg ggtttgaaat tttcaatttg
    10921 ttgcttaatt aaagcgctga tttcttgtgc gttaattgcc aaaagaacac cactttctat
    10981 ttcaaatttt ctttaacaac tttaagttgt tgtttaatac tcacatcaat tgtcttgtga
    11041 ttggcaaaaa tgacaaaacc accaatgaga ctttcatcga tttgttcttt tacactcctt
    11101 actttcagag acattttttt ctcaatcaaa gggagcaaac gagtcttctg ttcatcagtt
    11161 agaggatgag cagacgtaat cgtcacttca aatcgatttg tttctttttc aagtcggttc
    11221 aagcaatcta caagcacatc ataaaaaaga tttgctctat gattgtaggc cagaacctgg
    11281 ataaagtttt gtaataaagg tgacacagaa tcttggaaaa aagcaattgt tttttcctta
    11341 tccgactcgt ctactgccac ttgctttaaa aaagaaggca gacctgtttt ttcaacaact
    11401 tgcttgattt gagtcaagtc tgaaaagata cggtcttctt ctcctttttc aagtaccaat
    11461 tggacaaaag gcatgctata tttttcaatt acctttactg ttttcttgtc cattaagctt
    11521 ctcctagctg atcgatatac tgatcaatga gtgctttatg ggcatgactg tcaaggtttt
    11581 gtgagatgat tttaccagct aagctgatgg tcaaatctgc gacctcaccc ttaacactct
    11641 gtaaagcttc tactttattt tgagcaattt cttgattggc tttttctttt aagtgtcctg
    11701 cttctagttt agcatctgct aagatattag ccttactttg ctcagctgtt tcctttgcat
    11761 tttcaatgat tgtcttagct tctttacggc taccagccaa ttcatcttcg cgtttttgag
    11821 ccaatacttc tgctttttga cgggcttctt cagctctgtc aatatctgaa gcaatttttt
    11881 cagctctttc ttcgaaaatg cctgtaatat tagaccatgc aaatttttta atcaagacta
    11941 gcaaaagaat aaaagagcca gtgattaaaa taaaattacc aattaattca cctactgtta
    12001 cgtgcatcag ttactccttt ctattcctct tcaatattaa tcttattccc taaatacaca
    12061 gatgtcaaaa gagtaaaaac ataagcttgg atgcaggaaa taaagacaga aaatgcagtc
    12121 caagccaaat tagctccaaa ggctactgga taccaataaa tagcttggtg ggaaagaaga
    12181 agtaacaaac tcgtcatgac ctctcctgca aagatattcc caaaaatccg caaagccaaa
    12241 gataagaagt ttgtaaattc ttccaagata ttcatcggta tgacaaaaac aggactcata
    12301 aaagatttta tacttttttt aaatccacga cgacgaacgc tttctatatg tgtcaacaaa
    12361 atgacaagaa aagataaggt taagtcatac tgtaaattag cggttggcga actccaccag
    12421 ttagtcccat cgatcgtttg aagctttgtc attaagccaa ggttattggc aatcaccatg
    12481 aaaaggaata aacaaaggaa aaagagtgag taatctttca tgtagcgcga accaatgtta
    12541 ggttctgtaa atccaataac aaagtcatag acatactcaa gtacattttg ctttcctttg
    12601 ggtttcaagg tcatattgcg gcttgcccaa taaataaaga caaaaataac tcccacaatc
    12661 aacaaagtca tggctaacat agtcagattg aagataacag gaccaataga gatgattgga
    12721 ttaatacttt cttccatcta aaatcctccc ttttcttctc gttccatgta tttatttgat
    12781 aataaatgag aagacaagag ttacaaagaa agttccttca ataaaggcaa cacctaaaaa
    12841 catcaaacta cgaaactcag aaagcatatc tggttggcgt gctactgatt taaacagtcc
    12901 attcatcaat aaaccttcac cgacagatac gcccatacag gcaatacata agcctaaaaa
    12961 tgttaaattc ataacaaatt ctcctttaaa ataaaattta cttacttagt gtagtcctaa
    13021 aatttaaatt tgtcaacttt taaccgctat tggaagcgtt taataaaaat tatttccatt
    13081 ttgctaaatt tcaagttcaa gttagccatc cagaagtctt ctctgggtga cttgtagtcc
    13141 aagcattttt taggatagtt gttaatccac ttttcgatga atgcgacttc tttgggagtc
    13201 attttcttgg ttcccttagg taaccatcta cgaatgagcc tgttatgatt ctcattagtt
    13261 cccctttccc aagaggcata ggggtgcgca taatagatgt gctcctcaga aaatacatca
    13321 aacaagcgat tgaattccg
//
LOCUS       DBERDK010000049        13221 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.049, whole genome shotgun sequence.
ACCESSION   DBERDK010000049 DBERDK010000000
VERSION     DBERDK010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 13221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13221
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.049"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            124..2289
                     /locus_tag="ACL73L_001464"
     CDS             124..2289
                     /locus_tag="ACL73L_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HGS0772426.1"
                     /translation="MKKKFLAFLLILFPIFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKISKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALSEMKKDGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQAGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTVGVKNGTASQTFLTENQ
                     SKYGYKIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQGQKLKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRVISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQATKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYAILAIFYLVIITLLTRLAKRLEKRIR"
     gene            2289..3029
                     /locus_tag="ACL73L_001465"
     CDS             2289..3029
                     /locus_tag="ACL73L_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGS0772427.1"
                     /translation="MAKLKIDVNDLHKHYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEKLGMELLEKVGLADKANANPDSLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPRLKEFLDKVLNV"
     gene            3178..4665
                     /gene="zwf"
                     /locus_tag="ACL73L_001466"
     CDS             3178..4665
                     /gene="zwf"
                     /locus_tag="ACL73L_001466"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HGS0772428.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYKSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDSEHYIALRQLQAELN
                     EKYQAEHNKLFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYATANKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDQSGALRDMVQNHTLQLLSLLAMDKPASFTKDEIRAEKIKVFKNLYHPTD
                     EELKEHFIRGQYRSGKIDGMKYISYRSEPNVNPESTTETFTSGAFFVDSDRFRGVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTDATATGASPEPYEKLIYDVLNNNSTNFSHWDEVCASWKLIDRIEKLWA
                     ENGAPLHDYKAGSMGPQASFDLLEKFGAKWTWQPDITYRQDGRLE"
     gene            complement(4711..5988)
                     /gene="ftsY"
                     /locus_tag="ACL73L_001467"
     CDS             complement(4711..5988)
                     /gene="ftsY"
                     /locus_tag="ACL73L_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HGS0772429.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEDVDPTFTEVEEVS
                     QEEAEVEIVEQAVFQEEEIQDTVEESLDLEPVVEVSQKEVEEFPHSEEGNTEFLETIE
                     ENNSEVLEPERPQAEETVQEKYDRSLKKTRTGFGARLNAFFANFRSVDEEFFEELEEL
                     LIMSDVGVQVASNLTEELRYEAKLENAKKPDALRRVIIEKLVELYEKDGSYDESIHFQ
                     DNLTVMLFVGVNGVGKTTSIGKLAHRYKQAGKKVMLVAADTFRAGAVAQLAEWGRRVD
                     VPVVTGPEKADPASVVFDGMERAVAEGIDILMIDTAGRLQNKDNLMAELEKIGRIIKR
                     VVPEAPHETFLALDASTGQNALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAIREEL
                     NIPVKLIGFGEKIDDIGEFNSENFMKGLLEGLI"
     gene            complement(5992..6810)
                     /locus_tag="ACL73L_001468"
     CDS             complement(5992..6810)
                     /locus_tag="ACL73L_001468"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HGS0772430.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKETLKAARDRGIKVVLTT
                     GRPIKAMDFFLHELGTDGQEDEYTITFNGGLVQKNTGEILDKTVFSYDDVARLYEETE
                     KLSLPLDAISEGTVYQIQSDQESLYAKFNPALTFVPVDFEDLSSQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPKNVHKATGLAKLISHLGIDQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVPEVKAAANVVTPMTNDEEAVAWAIEEYVLKEN"
     gene            complement(6810..7604)
                     /locus_tag="ACL73L_001469"
     CDS             complement(6810..7604)
                     /locus_tag="ACL73L_001469"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HGS0772431.1"
                     /translation="MTIKLVATDMDGTFLDGNGRFDMDRLKSLLASYKEKGIYFAVAS
                     GRGFLSLEKLFADVRDDIIFIAENGSLVEYQGQDLYEATMSRDFYLATFEKLKTSPYV
                     DINKLLLTGKKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEAGEAWVNEHVPGVKAMTTGFESIDIVLDYVDKGVAIVELAKKLGITMDQVMAFGDN
                     LNDLHMMQVVGHPVAPENARPEILELAKTVIGHHKERSVIAYMEGL"
     gene            complement(7601..11140)
                     /gene="smc"
                     /locus_tag="ACL73L_001470"
     CDS             complement(7601..11140)
                     /gene="smc"
                     /locus_tag="ACL73L_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HGS0772432.1"
                     /translation="MYLKEIEIQGFKSFADKTKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDVIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HIYRSGDSEYKIDGKKVRLRDIHDLFLDTGLGRDSFSIISQGKVEEIFNSKPEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENARKFLDL
                     EGQRKAIYLDVLVAQIKENKAELESTEEELAQVQELLMSYYQKREKLEEENQTLKKQR
                     QDLQAEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLAALEDKRNS
                     LSKEKYDKESSLALLEGNLVQNNQKLNRLEAELLAFSDDPDQMIELLRERFVALLQEE
                     ADVSNQLTRIENELENSRQLSQKQADQLEKLKEQLATAKEKASQQKDELETAKVQVQK
                     LLADYQAIAKEQEEQKTSYQAQQSQLFDRLDSLKNKQARAQSLENILRNHSNFYAGVK
                     SVLQEKDRLGGIIGAVSEHLTFDVYYQTALEIALGASSQHIIVEDEESATKAIDFLKR
                     NRVGRATFLPLTTIKARTISSQNQDAIAVSPGFLGMADELVTFDTRLEAIFKNLLATT
                     AIFDTVEHAREAARQVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIFIKPELEQLQK
                     EIAADEASLGSEEAALKTLQDQMAALTERLEAIKSQGEQARIQEQGLSLAYQQTSQQV
                     EELETLWKLQEEEIDRLSEGDWQADKEKCQESLATIASEKQNLEAEIEEIKSNKNAIQ
                     ERYQNLQEEVAQARLLKTELQGQKRYEVADIERLGKELDNLNIEQEEIQRMLQEKVDN
                     LEKVDTELLSQQAEESKTQKTNLQQGLIRKQFELDDIEGQLDDIASHLDQARQQNEEW
                     IRKQTRAEAKKEKVSERLRHLQNQLTDQYQISYTEALEKAHELENLNLAEQEVQDLEK
                     AIRSLGPVNLEAIDQYEEVHNRLDFLNSQRDDILSAKNLLLETITEMNDEVKERFKST
                     FEAIRESFKVTFKQMFGGGQADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGVTMQESGVSKIVSVKLKDLESIEG"
     gene            complement(11131..11829)
                     /gene="rnc"
                     /locus_tag="ACL73L_001471"
     CDS             complement(11131..11829)
                     /gene="rnc"
                     /locus_tag="ACL73L_001471"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HGS0772433.1"
                     /translation="MKELQTVLKNHFEIEFADKKLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFDQFIKL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKDVAKVKEFIYQVMIPKVEAGEFEMITD
                     YKTHLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
     gene            complement(12012..12083)
                     /locus_tag="ACL73L_001472"
     tRNA            complement(12012..12083)
                     /locus_tag="ACL73L_001472"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12048..12050),aa:Arg,seq:ccg)
     gene            complement(12147..13133)
                     /locus_tag="ACL73L_001473"
     CDS             complement(12147..13133)
                     /locus_tag="ACL73L_001473"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HGS0772434.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENVAEQLAKGGYFYIMHRFDEAGRIPFIKRMHDQGLIASISVGVKDYEY
                     DFVSQLKADAPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMIMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPAKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
BASE COUNT         3754 a         3015 c         2437 g         4015 t
ORIGIN      
        1 atagcaattc aagaaagaaa aggaaaaatc aatgttatat tttctgacac gaaaaatata
       61 acaatttgcc tttttttact ttttctgata taatgagaga atattcggaa aaggagacta
      121 aaaatgaaga aaaaatttct agcatttttg ctaattttat tcccaatttt ctcattaggt
      181 attgccaaag cagaaacgat taagattgtt tctgataccg cctatgcacc ttttgagttt
      241 aaagattcag atcaaactta taaaggaatt gatgttgaca ttattaacaa agtcgctgag
      301 attaaaggct ggaacattca gatgtcctat cctggatttg acgcagcagt caatgcggtt
      361 caagctgggc aagccgacgc tatcatggca gggatgacaa agactaaaga acgtgaaaaa
      421 gtcttcacca tgtctgatac ttactatgat acaaaagttg tcattgctac tacaaagtca
      481 cacaaaatta gcaagtacga ccaattaact ggcaaaaccg ttggtgttaa aaacggaact
      541 gccgctcaac gtttccttga aacaatcaaa gataaatatg gctttactat taaaacattt
      601 gacactggtg atttaatgaa caacagcttg agtgctggtg ccatcgatgc catgatggat
      661 gacaaacctg ttatcgaata tgccattaac caaggtcaag acctccatat tgaaatggat
      721 ggtgaagctg taggaagttt tgctttcggt gtgaaaaaag gaagtaaata cgagcacctg
      781 gttactgaat ttaaccaagc cttgtctgaa atgaaaaaag atggtagtct tgataaaatt
      841 atcaagaaat ggactgcttc atcatcttca gcagtgccaa ctacaactac tctcgcagga
      901 ttaaaagcta ttcctgttaa ggctaaatat atcattgcca gcgattcttc ttttgcccct
      961 tttgttttcc aaaattcaag caaccaatac actggtattg atatggaatt gattaaggca
     1021 atcgctaaag accaaggttt tgaaattgaa atcaccaacc ctggttttga tgctgctatc
     1081 agtgctgtcc aagctggtca agccgatggt atcatcgctg gtatgtctgt cacagatgct
     1141 cgtaaggcaa cttttgactt ctcagaatca tactacactg ctaataccat tcttggtgtc
     1201 aaagaatcaa gtaatattgc ttcttatgaa gatctaaaag gaaagacagt cggtgttaaa
     1261 aacggaactg cttctcaaac cttcctaaca gaaaatcaaa gcaaatacgg ctacaaaatc
     1321 aaaacctttg ctgatggttc ttcaatgtat gacagtttaa acactggtgc cattgatgcc
     1381 gttatggatg atgaacctgt tctcaaatat tctatcagcc aaggtcaaaa attgaaaact
     1441 ccaatctctg gaactccaat cggtgaaaca gcctttgccg ttaaaaaagg agcaaatcca
     1501 gaactgattg aaatgttcaa caacggactt gcaaacctta aagcaaacgg tgaattccaa
     1561 aagattcttg acaaatacct agctagcgaa tcttcaactg cttcaacaag tactgttgac
     1621 gaaacaacgc tctggggctt gcttcaaaac aactacaaac aactccttag cggtcttggt
     1681 atcactcttg ctctagctct tatctcattt gctattgcca ttgtcatcgg aattatcttc
     1741 ggtatgttta gcgttagccc atacaaatct cttcgcgtca tctctgagat tttcgttgac
     1801 gttattcgtg gtattccatt gatgattctt gcagccttca tcttctgggg aattccaaac
     1861 ttcatcgagt ctatcacagg ccaacaaagc ccaattaacg actttgtagc tggaaccatt
     1921 gccctctcac tcaatgcggc tgcttatatc gctgaaatcg ttcgtggtgg tattcaggcc
     1981 gttccagttg gccaaatgga agccagccga agcttgggta tctcttatgg aaaaaccatg
     2041 cgtaagatta tcttgccaca agcaactaaa ttgatgttgc caaactttgt caaccaattc
     2101 gttatcgctc ttaaagatac aactatcgta tctgctatcg gtttggttga actcttccaa
     2161 actggtaaga ttatcattgc tcgtaactac caaagtttca agatgtatgc aatccttgct
     2221 atcttctatc ttgtaattat cacacttttg actagactag cgaaacgctt agaaaagagg
     2281 attcgttaat ggcaaaatta aaaattgatg taaatgattt acacaagcac tatggaaaaa
     2341 atgaagtcct aaaaggaatt acgactaagt tctatgaagg agatgttgtt tgtatcatcg
     2401 gtccttcagg ttctggtaag tcaactttcc tccgtagcct caatctttta gaagaagtca
     2461 ctagcggtca catcactgtg aacggctatg atttaactga aaaaacaacc aatgttgacc
     2521 acgtccgtga aaatatcggc atggtattcc aacacttcaa cctcttccct catatgtctg
     2581 tattggacaa catcaccttt gctcctattg agcacaagtt gatgactaag gaagaagctg
     2641 agaaattggg aatggagttg cttgaaaagg ttggactagc agataaagct aatgccaatc
     2701 cagatagcct atcaggtggt caaaaacaac gtgtggccat agctcgtggc ctagcaatga
     2761 atccagacat catgctcttc gatgaaccaa cttctgccct tgaccctgag atggttggag
     2821 acgtacttaa cgttatgaag gaattggctg agcaaggcat gaccatgatt atcgtaaccc
     2881 atgagatggg atttgctcgt caggttgcca accgcgttat ctttactgca gatggcgagt
     2941 tccttgaaga cggaacacct gaccaaatct ttgataaccc acaacaccct cgtctgaaag
     3001 agttcttaga taaggtctta aacgtctaaa ctcaaactgt aaggatttcc ttgcagtttt
     3061 tctacctcgt attggaattt ttgatttttc ggaaaattat gttagaatta agtttatgaa
     3121 atgaggtttc ctcataccta gcaagactag gaataaaaat agaaattagg tagctagatg
     3181 tcatctaagg ttattgttac aattttcggt gcgagtggag acctggctaa acgcaagctc
     3241 tacccttccc tttttagact atataaatcc ggcaatcttt ccaagcactt tgccgttatt
     3301 ggaactgccc gtagaccttg gagtaaggaa tattttgaat ctgtagttgt cgagtccatc
     3361 cttgatttgg cagatagtac cgagcaagcc caagaatttg ctagccactt ctactatcaa
     3421 agccatgatg tcaatgattc ggaacattat attgctttgc gtcaattaca agctgagctt
     3481 aatgaaaaat accaagctga acacaataag ctcttcttct tgtctatggc acctcagttc
     3541 tttggaacca ttgccaaaca cctcaaatct gaaaacattg tcgatggcaa aggttttgag
     3601 cgcttgatcg ttgaaaaacc atttggtaca gattacgcaa ctgcaaacaa gttgaatgac
     3661 gaactcctag caacatttga cgaagaacaa attttccgta tcgaccatta tcttggtaag
     3721 gaaatgatcc aaagcatctt tgcagttcgc tttgcaaact tgatttttga aaacgtttgg
     3781 aacaaggatt ttatcgacaa tgttcaaatt acctttgcgg agcgcttggg tgtagaagaa
     3841 cgtggtggct actatgacca atccggtgcc ctccgtgaca tggtccaaaa ccacactcta
     3901 caacttcttt cgctcctcgc catggacaaa ccagcaagct tcacaaaaga cgagattcgt
     3961 gctgaaaaga ttaaggtctt taaaaacctc tatcatccaa ctgatgaaga actcaaagaa
     4021 cactttatcc gtgggcaata ccgctctggt aagattgatg gcatgaaata catctcttat
     4081 cgtagcgagc caaatgtgaa tccagaatca acaactgaaa cctttacatc tggtgccttc
     4141 tttgtagaca gcgatcgatt ccgtggtgtt cctttctttt tccgtacagg taaacgactg
     4201 actgaaaaag gaactcatgt caacatcgtc tttaaacaaa tggattctat ctttggagaa
     4261 ccacttgctc caaatatttt gaccatctat attcaaccaa cagaaggctt ctctcttagc
     4321 ctaaatggga agcaagtagg agaagaattt aacttggctc ctaactcact tgattaccgt
     4381 acagatgcga ctgcaactgg tgcttctcca gaaccatacg aaaaattgat ttatgatgtc
     4441 ctaaataaca actcaactaa ctttagccac tgggatgaag tttgtgcgtc atggaagttg
     4501 attgaccgta ttgaaaagct ctgggctgaa aatggtgccc cacttcatga ctataaagct
     4561 ggaagcatgg gacctcaagc cagctttgac ctacttgaaa aattcggtgc caaatggact
     4621 tggcaaccag atatcaccta tcgtcaagat ggtcgcttag aataaaaaaa tttcctgcaa
     4681 gtttatgcct tgcaggattt ttgcttctga ttagattaaa ccttccaaga gacctttcat
     4741 aaagttttct gagttaaact ctccaatatc atcgattttt tcaccaaaac caatcaattt
     4801 tacaggaata ttgagttctt cacgaatggc tagaaccaca cctcctcgag cagttccatc
     4861 aatcttagtc aaaacaattc ccgttaaagg tgtgattttc gaaaattctt tggcctgtac
     4921 tagggcattt tgacctgttg atgcatcaag tgccaagaag gtttcatgtg gtgcttctgg
     4981 cacaacacgt ttgataatac gaccaatctt ttccaactca gccataaggt tatccttatt
     5041 ttgcagacga ccagcagtat caatcatgag aatatcgata ccttcagcca cggcacgttc
     5101 cataccatca aagaccacgc tggctggatc agctttttca ggtccagtta ctactggaac
     5161 atctactcgt cggccccatt cagctagctg agctactgca cccgcacgga aggtatctgc
     5221 tgcaaccagc atgaccttct taccagcttg tttgtagcgg tgggctagtt ttccgataga
     5281 agttgttttc ccaacaccat tcacaccaac aaagagcata actgtcaagt tatcttggaa
     5341 gtggatgctt tcatcgtagc taccatcctt ttcataaagc tcaaccaatt tctcaatgat
     5401 gacacgacga agtgcatcag gtttcttggc attttcaagc ttggcttcgt aacgtagttc
     5461 ctccgttaag ttagaagcga cttggacacc aacatcactc ataatcagca gttcttccag
     5521 ttcctcgaaa aattcttcgt caacagagcg gaagttagca aagaaggcat tcaagcgggc
     5581 accgaaacct gtacgagttt tcttaagact gcggtcatat ttttcctgaa cggtttcttc
     5641 tgcttgaggc ctttctggtt caagaacttc agaattattt tcttctatag tctctagaaa
     5701 ctcagtattc ccttcttctg agtgtggaaa ttcttcgact tctttttgag aaacttctac
     5761 aactggctct aaatccagac tttcttcaac tgtgtcttgg atttcctctt cttggaacac
     5821 agcttgttca acaatttcaa cctctgcttc ttcctgagaa acttcctcaa cttctgtgaa
     5881 ggtaggatca acatcttcag acaaatcaag attttccaga gcttctttta caacttcttc
     5941 gattttaggt tcttcttttt ttccgaatag acggtcaaac aatcccatat cttagttctc
     6001 ctttagcaca tattcttcga tagcccaggc gacagcttcc tcatcgttgg tcatcggcgt
     6061 cactacattt gcggctgcct ttacttcagg aacagcgttt tgcatagcaa caccaagacc
     6121 tgcccattca atcatagaga ggtcattggc ctcgtcacca caagccatca cttgactttg
     6181 gtcgattcca agatggctga ttagttttgc caaacctgtt gctttatgaa cattctttgg
     6241 tgaccattct agcaacattt cacgtgattt aaagatttca tattggtcaa acaattctgg
     6301 agaaatcttc tgaatggctg catccaaggg ttcttgagca aaggcagtca cgcatttgtt
     6361 gtaggtcatt tgactagata agtcttcaaa gtccactgga acaaaggtca aagctggatt
     6421 gaatttggca taaagacttt cttggtccga ttggatttga taaactgttc cttctgagat
     6481 ggcatcaaga ggcagtgata atttctctgt ttcttcatac aaacgtgcca catcatcata
     6541 tgaaaagact gttttatcaa ggatttctcc tgtatttttc tgaactaatc caccattaaa
     6601 agtaatggta tactcatctt cctgaccgtc agtccctaac tcatggagaa agaaatccat
     6661 ggcttttatg ggacgaccag ttgtcaatac gaccttgata ccacgatcac gcgcagcttt
     6721 caaggtttcc ttggtacgat ccgtcagcct tttatcagta gtcagcaagg tcccgtccaa
     6781 gtccaatgca atcaatttta tatctgccat tataagccct ccatataagc tataaccgac
     6841 cgttccttat ggtgaccaat cacagtcttt gctaattcta aaatttcagg tcgtgcattt
     6901 tcaggagcta caggatgtcc cacaacctgc atcatatgta agtcattaag attgtctcca
     6961 aaagccatga cctgatccat tgtgatacca agttttttag ctaattcaac aatagctaca
     7021 cccttatcga catagtccag aacaatatca atagattcaa agccagttgt catggcctta
     7081 acaccaggga catgttcatt aacccaagct tcaccagctt ctagcgtttc ttctgtgaag
     7141 ttggttgtaa atttgaaaat gtcatctgtg atatcttcca aactcgctac tttttggata
     7201 ttttcattat aatgctgact cactttcaaa taggtctcat caaccgtatc tagaacatat
     7261 gaacccttct tacccgtcaa gagcagttta ttgatatcta cataaggtga cgttttcagc
     7321 ttttcaaaag ttgccagata aaagtcacga gacatagtcg cttcatacaa gtcctgacct
     7381 tgatactcta ccaaactgcc attttccgcg atgaaaataa tgtcatcacg aacatcagca
     7441 aataattttt ccagagacag aaatccccga cccgaagcca ccgcaaagta aatccctttt
     7501 tccttgtagg aagccaagag agacttgaga cgatccatat caaagcgtcc attcccatct
     7561 aggaaggttc cgtccatatc cgttgctact agtttaattg tcatccttca atactttcta
     7621 aatcttttaa cttaactgaa acaatctttg aaacacccga ttcttgcatg gtcactccat
     7681 agatggaatc agccgctgcc atggttccct tacggtgggt tacaacgata aactggctgt
     7741 ccttgtcaaa acggttgagg taatccccaa aacgtttaac attggcttca tccagcgcag
     7801 cttccacctc atccaagatg acaaaaggaa tggtcttgac acgaataatg gagaaaagca
     7861 aggcaagagc cgatagagct ttttcaccac cactcatgag gttaagcgac tggattttct
     7921 tacctggagg ttgaacagaa atctccacac cagctgttaa aaggtcgccc tcagtcaata
     7981 tcaagtctgc ctgacctccg ccaaacatct gcttgaaggt cactttaaag gactcacgaa
     8041 tagcttcaaa ggttgattta aagcgttcct tgacctcatc attcatctct gtaatggttt
     8101 caaggagcag atttttcgct gacaaaatat catctcgctg actatttaga aagtccagac
     8161 ggttgtgaac ttcttcgtac tggtcaatag cttccaagtt gacaggtccc aatgagcgaa
     8221 tagccttctc taaatcctgc acctcttgct ctgccagatt gaggttttcc aattcatgtg
     8281 ccttttctag tgcttcagta tagctaatct ggtactggtc tgttaattga ttttgtagat
     8341 ggcgcaagcg ctcgctgacc ttttctttct tggcttcagc acgtgtttgc ttgcgaatcc
     8401 actcctcatt ctgctggcga gcttgatcca agtgactggc aatatcatcc agttgacctt
     8461 ctatatcatc caactcaaac tgcttgcgaa tcaaaccttg ttggagattt gttttctgag
     8521 ttttggattc ttccgcctgt tgactgagca attctgtatc aaccttctca agattgtcaa
     8581 ctttttcttg gagcatgcgc tgaatttctt cttgttcgat attaagattg tccaattcct
     8641 tgcctaaacg ctcaatatca gctacttcat aacgtttttg cccttgcagt tctgtcttaa
     8701 gcaagcgagc ttgcgccacc tcttcctgca aattttgata gcgttcttgg atggcgtttt
     8761 tattagactt aatttcttca atctcagctt ccagattttg cttttcactg gcgatagtag
     8821 caaggctctc ttgacatttt tccttatccg cttgccaatc tccctcagaa agacgatcta
     8881 tttcctcttc ttggagtttc caaagagttt ccagttcttc aacttgctga ctagtttgct
     8941 gataagcgag ggacaagcct tgctcctgaa tacgtgcctg ctctccttga gatttgatgg
     9001 cttctaatct ttctgtcaat gcagccatct ggtcttgcaa ggtcttcaaa gccgcttctt
     9061 ctgaacccaa gcttgcttca tctgcagcaa tttctttttg taattgctcc agttctggct
     9121 tgatgaaaat actgttattt tggcgattgg caccacccgc ataggaacca cccgtgcgta
     9181 attctgtccc atccaatgtc accatacgaa cctgataacg aacttgtcga gcagcttcac
     9241 gcgcatgttc tacggtatca aagatagccg tcgtagctag caagttcttg aaaatggctt
     9301 ccagtctagt atcaaaagtc accaactcat ctgccatccc aaggaaacct gggcttacag
     9361 cgatagcatc ttggttctga ctagaaatcg tacgcgcctt aatagtggtc aaaggaagaa
     9421 aggttgcacg accgactctg tttcgtttga ggaaatcaat agctttggtt gccgactctt
     9481 catcttctac gatgatatgc tggctacttg cccctaaggc aatctctagg gcagtttgat
     9541 aataaacatc aaaggtcaga tgctcactga ctgcaccaat aatcccacct aggcgatctt
     9601 tttcttggag aacactctta acacctgcat aaaagttact atgatttctc aggatatttt
     9661 ccaaactttg agctctggcc tgcttgtttt tgagactatc cagacggtca aagagttgac
     9721 tttgttgagc ttgataggaa gttttctgct cctcttgctc cttggcaata gcttgatagt
     9781 cagccaataa tttctgaacc tgcaccttgg cagtttcaag ctcgtctttt tgctgactag
     9841 ccttctcttt agctgtagct aattgctctt tcagcttttc tagttgatct gcttgttttt
     9901 gagaaagctg acgactattt tccaactcat tctcaatacg ggtcaactgg tttgagacat
     9961 ccgcttcttc ttgtaaaaga gctacaaagc gttcacgtaa gagctcaatc atctgatcag
    10021 gatcgtctga gaaagccagc aattcagctt ctaaacgatt gagtttttga ttattttgga
    10081 ctagatttcc ctctaacaga gctaaagagc tttctttatc atacttttct ttgctgagtg
    10141 aatttctctt atcctccaaa gcagccaaac gagcttgtgc ctcctgttga ttcagggcca
    10201 cttgctcgga ctccagtttc gatagggcta attttctttc taaatcacta atcagactag
    10261 tcaagtccat caaactgcct tggtctttgg ccatttcagc ctgtaaatct tggcgttgct
    10321 ttttaagagt ttgattttct tcttctaatt tttcacgctt ttggtaataa ctcatcaaga
    10381 gttcttgaac ctgagccaac tcttcttctg tcgactctag ttctgcctta ttttccttga
    10441 tttgagcaac cagaacgtct aaataaatag ccttacgttg tccttccaag tctaaaaact
    10501 tacgggcatt ctcagcttgc ttctcaagag gcttgatttg attatccaac tcgtagataa
    10561 tgtcctctaa gcggtccaga ttatcctgag tttgctgcag tttactctcg gtttcttttc
    10621 tgcgagtctt gtattttaaa actccagcag cttcttcaaa aatagctcgt cgttcctcag
    10681 gcttggaatt aaaaatctcc tcaaccttcc cttgggaaat aatagagaag gaatctcgtc
    10741 ccaatccagt atccaagaag aggtcatgaa tatcacgcag acggactttc ttgccgtcaa
    10801 tcttgtattc gctatctcca ctacgataga tatggcgttc caccctgatt tcttgacctg
    10861 catccttgat aaatccgtca tgattatcca gagtcacaac tacagaagca taattgagcg
    10921 gtttgcgact ttctgttcca gcaaagatga catccggcat cttgccccca cggagactct
    10981 tgacactaga ctcccccaaa gcccaacgca gactttctgt aatattggac tttccagatc
    11041 cattgggtcc aacaactgcc gtcacacctt ggtcaaaaac gaccttggtc ttatcagcaa
    11101 aagacttgaa cccctgaatt tcgatttcct ttaaatacat gaatccagcc ctttctcaac
    11161 ggcatttttg gcagcttcct gctctgctaa tttcttagaa cgaccttggc cttgaccgat
    11221 gctcttacct tcaacaagaa cttctacatc aaagacctta tcgtgagcag gccctgtttc
    11281 agaaatcacc tgataacgaa tagccacatc accattgacc tgaagcaact cttggagatg
    11341 ggttttatag tctgtaatca tctcaaattc gcctgcttct accttaggaa tcataacttg
    11401 gtagataaat tccttgacct ttgccacatc cttatccaaa agaagggcac caagaaaggc
    11461 ttcaaaggca tcaccaagaa tggtgtcacg attgcgacca ccagattttt cttccccttt
    11521 acccaacttg ataaactggt caaactggca atcacgcgca aaaccagcta aactctcctc
    11581 acggacaatc atagcacgga gttttgatag gtcaccttca ggctttttag gatatttttt
    11641 atatagatat tctgaaatca ataactgtag aacagcgtct cctaaaaatt ccaagcgttc
    11701 attgtgtgaa atttttaaga ggcggtgctc attggcataa ctcgtatgag taaaggcagt
    11761 ttccagtaac tttttgtctg caaattcgat ttcaaaatga ttctttagta cagtttgtaa
    11821 ttctttcata ccaacttctt tctaactgat aatagtcctt tttattatat caaaaaaagc
    11881 cccctgagtc actctaaaac gggactggaa agcatttggg aattctttag acagagattc
    11941 tcagttttag cggcaaattt gggtcaggat aaagaaaaaa gccctattaa aggcttttta
    12001 ggatgtttac atccaccctg agggaatcga acccccatct caagaaccgg aatcttacgt
    12061 gatatccatt acactaaggg tggaaacttg ttttattata acagaaattt gctctaataa
    12121 caagtttttt ggtcaaagac cccgtcttag tgggaagcat ccccattcca gatggagttt
    12181 ttcacgatca cataatcaac gtgtttaagg tcagcaacct gacgtccacc tgcataagaa
    12241 atagcacttt gaaggtcttg ttccatctca gttaaagtgt cttgcagatg acctttagca
    12301 ggaagcaaga tacgtttgcc ttccacattt ttgtaagcac ctttttgata ttgtgaggct
    12361 gaaccataat attctttgaa ctgttcacca tcgacttcaa tcgttttccc tggactttca
    12421 atgtgtcctg caaagaggga accaatcatg atcatgctag caccgaagcg gatagacttg
    12481 gcaatatcac cgtgagtacg aattcctcca tcagcgataa tcggtttacg cgcagccttg
    12541 gcacaccagc gtagagcagc caactgccaa ccacctgtac caaaaccagt cttaaccttg
    12601 gtgatacaaa ccttaccagg accgattccg accttagtag catccgcacc agcattttcc
    12661 aattcacgca cagcttctgg tgttcccaca tttccagcaa tgacaaaggt atctggcaat
    12721 tctttcttga tgtgttgaat catagaaatc acgctatccg catgaccatg agcaatatca
    12781 atagtgatat actcaggagc atcagccttg agctggctaa caaaatcata ctcatagtcc
    12841 ttaacaccga cagagataga agcaatgagt ccttgatcgt gcatgcgttt aataaaagga
    12901 atgcgtcctg cctcatcaaa acggtgcata atgtagaagt aaccaccttt agccagttgc
    12961 tctgctacat tttcatccaa aatcgtctgc atattcgctg gcacaacagg tagtttaaag
    13021 gtgtgatttc ctaaagtgac acttgtatcc gcttctgcac ggcttttaat gacacattta
    13081 tttggaatca attgaatatc ttcgtaatca aaaattggaa attcatttaa catatcgatg
    13141 tctcgtttct tttgtaatga cctacctatg ctctcgcatc actacgcctt ttccttaatt
    13201 tattataaac taaagtacag t
//
LOCUS       DBERDK010000050        12714 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.050, whole genome shotgun sequence.
ACCESSION   DBERDK010000050 DBERDK010000000
VERSION     DBERDK010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 12714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12714
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.050"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            26..1162
                     /gene="dnaN"
                     /locus_tag="ACL73L_001474"
     CDS             26..1162
                     /gene="dnaN"
                     /locus_tag="ACL73L_001474"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HGS0772435.1"
                     /translation="MIHFSINKNLFLQALNTTKRAISSKNAIPILSTVKIDVTNEGIT
                     LIGSNGQISIENFISQKNEDAGLLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQN
                     QIVLTSGKSEITLKGKDSEQYPRIQEISASTPLILETKLLKKIINETAFAASTQESRP
                     ILTGVHFVLSQHKELKTVATDSHRLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDD
                     IETVEIFFANNQILFRSENISFYTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSME
                     RARLLSSATQNGTVKLEIKDGVVSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYL
                     IDSLKALNSEKVTISFISAVRPFTLVPADTDEDFMQLITPVRTN"
     gene            1227..1421
                     /locus_tag="ACL73L_001475"
     CDS             1227..1421
                     /locus_tag="ACL73L_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HGS0772436.1"
                     /translation="MYQVGNFVEMKKPHACTIKSTGKKANRWEITRVGADIKIKCSNC
                     EHVVMMGRYDFERKINKIID"
     gene            1505..2620
                     /gene="ychF"
                     /locus_tag="ACL73L_001476"
     CDS             1505..2620
                     /gene="ychF"
                     /locus_tag="ACL73L_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HGS0772437.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELDDEDKKEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQAAGIIHSDFEKGFIRAVTMSYED
                     LVKYGSEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            2691..3260
                     /gene="pth"
                     /locus_tag="ACL73L_001477"
     CDS             2691..3260
                     /gene="pth"
                     /locus_tag="ACL73L_001477"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HGS0772438.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     QADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTQVFNRVKIGIGRPKNGMSVVHHVLSKFDRDDYIGI
                     LQSVDKVDDSVNYYLQEKKFEKTMQRYNG"
     gene            3261..6770
                     /gene="mfd"
                     /locus_tag="ACL73L_001478"
     CDS             3261..6770
                     /gene="mfd"
                     /locus_tag="ACL73L_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HGS0772439.1"
                     /translation="MVTLLDLFSENDQIKKWHQNLTDKKRQLILGLSTSTKALAIASS
                     LEKEDRIVLLTSTYGEAEGLVSDLISILGEELVYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIAASRLILPSPNAFKDSIVKISVGEEYDQHAFIHQLKENG
                     YRKVTQVQTQGEFSLRGDILDIFEISQLEPCRIEFFGDEIDGIRSFEVETQLSKENKT
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADSRK
                     FLSLCYDKTWTVFDYIEKDTPIFFDDYQKLMNQYEVFERDLAQYFTEELQNSKAFSDM
                     QYFSDIEQIYKKQSPVTFFSNLQKGLGNLKFDKIYQFNQYPMQEFFNQFSFLKEEIER
                     YKKMDYTIILQSSNSMGSKTLEDMLEEYQIKLDSRDKTSICKESVNLIEGNLRHGFHF
                     VDEKILLITEHEIFQKKLKRRFRRQHVSNAERLKDYNELEKGDYVVHHIHGIGQYLGI
                     ETIEIKEIHRDYVSVQYQNGDQISIPVEQIHLLSKYISSDGKAPKLNKLNDGHFKKAK
                     QKVKNQVEDIADDLIKLYSERSQLKGFAFSADDDDQDAFDDAFPYVETDDQLRSIEEI
                     KRDMQASQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVVILVPTTVLAQQHYTNFK
                     ERFQNFAVNVDVLSRFRSKKEQTATLEKLKNGQVDILIGTHRVLSKDVVFADLGLMII
                     DEEQRFGVKHKETLKELKKQVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPV
                     QTYVLEKNDSVIRDAVLREMERGGQGYYLYNKVDTIVQKVSELQELIPEASIGYVHGR
                     MSEVQLENTLLDFIEGQYDILVTTTIIETGVDIPNANTLFIENADHMGLSTLYQLRGR
                     VGRSNRIAYAYLMYRPEKSISEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNLLG
                     KSQSGFIDSVGFELYSQLLEEAIAKRNGNANANTRIKGNAELILQIDAYLPDTYISDQ
                     RHKIEIYKKIRQIDNRVNYEELQEELIDRFGEYPDVVAYLLEIGLVKSYLDKVFVQRV
                     ERKDNKITIQFEKVTQRLFLAQDYFKALSVTNLKAGIAENKGLMELVFDVQNKKDYEI
                     LEGLLIFGESLLEIKESKEENSI"
     gene            6828..7094
                     /locus_tag="ACL73L_001479"
     CDS             6828..7094
                     /locus_tag="ACL73L_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HGS0772440.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIRFGNKLLLVKVLEMKDSTKKEDAAGMYEIISETRVEENV"
     gene            7087..7455
                     /locus_tag="ACL73L_001480"
     CDS             7087..7455
                     /locus_tag="ACL73L_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HGS0772441.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGGVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATKLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            7460..7582
                     /locus_tag="ACL73L_001481"
     CDS             7460..7582
                     /locus_tag="ACL73L_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0009 family protein"
                     /protein_id="HGS0772442.1"
                     /translation="MENLLDVIEQFLSLSDEKLEELADKNQLLRLQEEKERKNA"
     gene            7575..8843
                     /locus_tag="ACL73L_001482"
     CDS             7575..8843
                     /locus_tag="ACL73L_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HGS0772443.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFGIY
                     FREKLSSPMVYGEVPVYANEDLVVESGKLTPKTSFQITEWRLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKVWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQY
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKED
                     NKEYSLKDLITKVSKESDNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISS
                     KMAGKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYA
                     DSPFILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            8840..10117
                     /gene="tilS"
                     /locus_tag="ACL73L_001483"
     CDS             8840..10117
                     /gene="tilS"
                     /locus_tag="ACL73L_001483"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HGS0772444.1"
                     /translation="MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETILMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYD
                     LAIAELSNNINVEDLQQLFSYSESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKSKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEKEDELVLHYQNQVAYQGYLFSFGLPLEG
                     ELIQQIPVSRETSIHIRHRKTGDVLIQNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKTKNDIMNTVLYIEKIDR"
     gene            10121..10663
                     /gene="hpt"
                     /locus_tag="ACL73L_001484"
     CDS             10121..10663
                     /gene="hpt"
                     /locus_tag="ACL73L_001484"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HGS0772445.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKEREAASVKIATLLDKPEGRVVEIEADYTCFTIPNEFVVGYGLDY
                     KENYRNLPYIGVLKEEVYSN"
     gene            10679..12637
                     /gene="ftsH"
                     /locus_tag="ACL73L_001485"
     CDS             10679..12637
                     /gene="ftsH"
                     /locus_tag="ACL73L_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HGS0772446.1"
                     /translation="MKKQNNGLIKNPFLWLLFIFFLVTGFQYFYSGNNSGGSQQINYT
                     ELVQEITDGNVKELTYQPNGSVIEVSGVYKNPKTSKEGTGIQFFTPSVTKVEKFTSTI
                     LPADTTVSELQKLATDHKAEVTVKHESSSGIWINLLVSIVPFGILFFFLFSMMGNMGG
                     GNGRNPMSFGRSKAKAANKEDIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAAPAIIFIDEIDAVGRQRGVGLGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFV
                     GADLENVLNEAALVAARRNKSIIDASDIDEAEDRVIAGPSKKDKTVSQKERELVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGGR
                     VAEEIIFNVQTTGASNDFEQATQMARAMVTEYGMSEKLGPVQYEGNHAMLGAQSPQKS
                     ISEQTAYEIDEEVRSLLNEARNKAAEIIQSNRETHKLIAEALLKYETLDSTQIKALYE
                     TGKMPEAVEEESHALSYDEVKSKMNDEK"
BASE COUNT         4383 a         1913 c         2591 g         3827 t
ORIGIN      
        1 actaaaaata aataaaggag aatccatgat tcatttttca attaataaaa atttatttct
       61 acaagcatta aatactacta agagagctat tagttctaaa aatgccattc ctattttatc
      121 aacagtaaaa attgacgtga ccaatgaagg tattacttta attggttcaa atggtcaaat
      181 ttcaattgaa aattttattt ctcaaaaaaa tgaagatgct ggtttgttaa ttacttcttt
      241 aggttcgatc cttcttgaag cttctttctt tatcaatgta gtatctagtt tacctgatgt
      301 aactcttgat tttaaagaaa ttgaacaaaa tcaaattgtt ttaaccagtg gcaaatcaga
      361 aattacccta aaaggaaaag atagcgaaca atatccacga atccaagaaa tttcagcaag
      421 cactccttta atacttgaaa caaaattact caagaaaatt attaatgaaa cagcctttgc
      481 tgcaagtaca caagagagtc gtccgatttt aacaggtgtc cacttcgtat tgagtcaaca
      541 caaagagtta aaaacagttg caacagactc tcatcgccta agccagaaaa aattgactct
      601 tgaaaaaaat agtgatgatt ttgatgtcgt aattcctagc cgttctctac gcgaattttc
      661 agcggtattt acagatgata tcgaaactgt agagattttc tttgccaata accaaatcct
      721 ctttagaagc gaaaatatta gcttctatac tcgtctccta gaaggaaact atcctgatac
      781 agatcgcttg attccaacag actttaacac tactattact tttaatgtgg taaacttacg
      841 ccagtcaatg gagcgtgccc gtcttttatc aagtgcgact caaaatggta ctgtgaaact
      901 tgaaattaag gatggggttg ttagcgccca tgttcactct ccagaagttg gtaaagtaaa
      961 cgaagaaatc gatactgatc aggttactgg tgaagatttg accattagtt tcaacccaac
     1021 ttacttgatt gattctctta aagctttaaa tagcgaaaag gtgactatta gctttatctc
     1081 agctgttcgt ccatttactc ttgtgccagc agatactgac gaagacttca tgcagctcat
     1141 tacaccagtt cgtacaaatt aagtgaaaga ggttgagcct ggctcgcctc ttttatgata
     1201 taatcgaaaa agaaaaggag agtagtatgt atcaagttgg aaattttgtt gagatgaaaa
     1261 aaccacacgc ttgtacaatc aagtcgactg gtaaaaaggc taatcgttgg gaaattacac
     1321 gtgtaggagc agatatcaaa ataaaatgca gtaattgtga gcatgttgtc atgatggggc
     1381 gatatgattt tgagcgaaaa ataaataaaa ttattgactg agaaccctta gttagagggt
     1441 tagcacttta tctctttttg tgttataata ttagggattg aaatgaaaac ggagaatgag
     1501 aaatatggct ttgacagcag gtatcgttgg tttgccaaac gttggtaaat caacactatt
     1561 taatgcaatt acaaaagcag gagcagaggc agcaaactac ccatttgcga ctattgatcc
     1621 aaatgttgga atggtggaag ttccagatga acgcctacaa aaactaactg aaatgataac
     1681 tcctaaaaag acagttccca caacatttga atttacggat attgcaggga ttgtaaaagg
     1741 agcttcaaaa ggagaagggc tagggaataa attcttggcc aatattcgtg aagtagatgc
     1801 gattgttcac gtagttcgtg cttttgatga tgaaaatgtg atgcgcgagc aaggacgtga
     1861 agacgccttt gtagatccac ttgcagatat tgatacaatt aatctggaat taattcttgc
     1921 tgacttagaa tcagtgaaca aacgatatgc gcgtgtagaa aagatggcac gtacgcaaaa
     1981 agataaagaa tcagtagcag aattcaatgt tcttcaaaag attaaaccag tcctagaaga
     2041 cgggaaatca gctcgtacca ttgaatttac agatgaggaa caaaaggttg tcaaaggtct
     2101 tttccttttg acgactaaac cagttcttta tgtagctaat gtggacgagg atgtggtttc
     2161 agaacctgac tctatcgact atgtcaaaca aattcgtgaa tttgcagcga cagaaaatgc
     2221 tgaagtagtc gttatttctg cgcgtgctga ggaagaaatt tctgaattgg atgatgaaga
     2281 taaaaaagag tttcttgaag caattggttt gacagaatca ggtgtagata agttgacgcg
     2341 tgcagcttac cacttgcttg gattgggaac ttacttcaca gctggtgaaa aagaagttcg
     2401 cgcttggact ttcaaacgtg gtatgaaggc tcctcaagca gctggtatta tccactcaga
     2461 ctttgaaaaa ggctttattc gtgcagtaac catgtcatat gaagatctag tgaaatacgg
     2521 atctgaaaag gccgtaaaag aagctggacg cttgcgtgaa gaaggaaaag aatatatcgt
     2581 tcaagatggc gacatcatgg aattccgctt taatgtctaa aaattaataa atggtgtcaa
     2641 ttaggttgga aaaaaattcc aacccttttg gcttttgaaa ggaaaaataa atgaccaaat
     2701 tacttgtagg cttgggaaat ccaggggata aatattttga aacaaaacac aatgttggtt
     2761 ttatgttgat tgatcaacta gcgaagaaac agaatgtcac ttttacacac gataagatat
     2821 ttcaagctga cctagcatcc tttttcctaa atggagaaaa aatttatctg gttaaaccaa
     2881 cgacctttat gaatgaaagt ggaaaagcag ttcatgcttt attaacttac tatggtttgg
     2941 atattgacga tttacttatc atttacgatg atcttgacat ggaagttggg aaaattcgtt
     3001 taagagcaaa aggctcagca ggtggtcata atggtatcaa gtctattatt caacatatag
     3061 gaactcaggt ctttaaccgt gttaagattg gaattggaag acctaaaaat ggtatgtcag
     3121 ttgttcatca tgttttgagt aagtttgaca gggatgatta tatcggtatt ttacagtctg
     3181 ttgacaaagt tgacgattct gtaaactact atttacaaga gaaaaaattt gagaaaacaa
     3241 tgcagaggta taacggataa atggtgacct tattagattt attctcagaa aatgatcaga
     3301 ttaaaaaatg gcatcaaaat ttaacagata agaaaagaca actaatactt ggtttatcaa
     3361 catctactaa ggctcttgca attgcaagca gtttagaaaa agaagatagg attgtgttat
     3421 tgacgtcaac ttatggagaa gcagaaggac ttgttagtga tcttatttct atcttgggtg
     3481 aggaactcgt ctatccattt ttggtagatg atgctcctat ggtggagttt ttgatgtctt
     3541 cacaggaaaa aattatttca cgggttgaag ccttgcgttt tttgactgat tcatctaaga
     3601 aagggatttt agtttgtaat atcgcagcaa gtcgattgat tttaccgtct cccaatgcat
     3661 tcaaagatag tattgtaaaa atctcagttg gtgaagaata tgatcaacac gcgtttatcc
     3721 atcagttaaa ggaaaatggc tatcgaaaag ttactcaagt acaaactcag ggcgaattta
     3781 gtcttcgagg agatatttta gatatttttg aaatatccca gttagaacct tgtcgaattg
     3841 agttttttgg tgatgaaatt gatggtatca ggtcatttga agtagaaaca caattatcga
     3901 aagaaaataa gacagaactc actatctttc cagctagtga tatgcttttg agagaaaagg
     3961 attatcaacg aggacagtca gctttagaaa aacaaatttc aaaaacttta tcacctattt
     4021 tgaaatcata cctagaagaa attctttcaa gttttcacca aaaacaaagt catgcagact
     4081 ctcggaagtt tttatctttg tgctatgata agacatggac tgtctttgat tatattgaaa
     4141 aagatactcc aatattcttt gatgattatc aaaaattgat gaatcagtat gaagtctttg
     4201 aaagagactt agcgcagtac tttacagaag aattacagaa tagtaaagca ttttctgata
     4261 tgcagtattt ttctgatatt gaacaaatct ataaaaaaca aagtccagtg acctttttct
     4321 ctaatcttca aaagggttta ggaaatctca aatttgacaa aatttatcaa ttcaatcaat
     4381 atcctatgca ggaatttttc aatcagtttt cttttctaaa agaagaaatt gaacgatata
     4441 aaaaaatgga ttacaccatt attctgcagt ctagcaattc aatgggaagt aaaacattgg
     4501 aggatatgtt agaggaatat cagattaaat tggattctag agataagaca agtatctgta
     4561 aagaatctgt aaatttaata gagggtaatc tcagacatgg ttttcatttt gtagatgaaa
     4621 agattttatt gataactgaa catgagattt ttcaaaagaa attaaagcgt cgttttcgaa
     4681 gacaacatgt ttcaaatgca gagagattaa aagattacaa tgaacttgaa aaaggggact
     4741 atgttgtcca tcatatccat gggattggtc aatatctagg aattgaaacc attgaaatca
     4801 aggaaattca tcgtgattat gtcagtgtcc agtaccaaaa tggtgatcaa atttctattc
     4861 ccgtggaaca gattcatcta ctgtccaaat atatttcaag tgatggtaaa gctccaaaac
     4921 tcaataaatt aaatgacggt cattttaaaa aggccaagca aaaggttaag aaccaggtag
     4981 aggatatagc tgatgattta atcaaactct actctgaacg tagtcagttg aagggttttg
     5041 ctttctcagc tgatgatgat gatcaagatg cctttgatga tgctttccct tatgttgaaa
     5101 cggatgatca acttcgtagt attgaggaaa tcaagaggga tatgcaggct tctcagccaa
     5161 tggatcgact tttagttggg gatgttggtt ttggaaagac tgaagttgct atgcgtgcag
     5221 cctttaaagc agtcaatgat cacaaacagg ttgtcattct agttccgacg acggttttag
     5281 cgcaacagca ctatacgaat tttaaggaac gattccaaaa ttttgcagtt aatgttgatg
     5341 tgttgagtcg ctttagaagt aaaaaagagc agactgcaac acttgaaaaa ttgaaaaacg
     5401 gtcaagtcga tattttgatt ggaacacatc gtgttttgtc aaaagatgtt gtgtttgctg
     5461 atttgggctt gatgattatt gatgaggaac agcgatttgg tgtcaagcat aaggaaactt
     5521 tgaaagaact gaagaaacaa gtggatgtcc taaccttgac cgctacgcca atccctcgta
     5581 ccctccatat gtctatgctg ggaatcagag atttgtctgt tattgaaact ccgccgacta
     5641 atcgctaccc tgttcagacc tatgttttgg aaaagaatga tagtgtcatt cgtgatgctg
     5701 tcttgcgtga aatggagcgt ggaggtcaag gttactatct ttacaacaaa gttgacacaa
     5761 ttgttcagaa ggtttcagaa ttacaggagt tgattccaga ggcttcgatt ggatatgttc
     5821 atggtcgaat gagtgaagtc cagttggaaa atactctatt agactttatt gagggacaat
     5881 acgatatctt ggtgacgact actattattg agacaggggt ggacattcca aatgctaata
     5941 ctttatttat tgaaaatgcg gaccatatgg gcttgtcaac cttatatcag ttaagaggaa
     6001 gagtcggtcg tagtaatcgt attgcttatg cttatctcat gtatcgtcca gaaaaatcaa
     6061 tcagtgaagt ctctgaaaag agattagaag cgattaaagg atttacagaa ttgggctctg
     6121 gctttaagat tgcaatgcga gatctttcga ttcgtggagc aggaaatctt ttaggaaaat
     6181 cccagtctgg tttcattgat tctgttggtt ttgaattgta ttcgcagtta ttagaggaag
     6241 ctattgctaa acgaaacggt aatgctaacg ctaacacaag aatcaaaggg aatgctgagt
     6301 tgattttgca aattgatgcc tatcttcctg atacttatat ttctgatcaa cgacataaga
     6361 ttgaaattta caagaaaatt cgtcaaattg acaaccgtgt caattatgaa gagttacaag
     6421 aggagttgat agaccgtttt ggagaatacc cagatgtagt agcctatctg ttagagattg
     6481 gtttggtcaa atcatacttg gacaaggtct ttgttcaacg tgtggaaaga aaagataata
     6541 aaattacaat tcaatttgaa aaagtcactc aacgactgtt tttagctcaa gattatttta
     6601 aagctttatc cgtaacgaac ttaaaagcag gcatcgctga gaataaggga ttaatggagc
     6661 ttgtatttga tgtccaaaat aagaaagatt atgaaatttt agaaggtttg ctgatttttg
     6721 gagaaagttt attagagata aaagagtcta aggaagaaaa ttccatttga tatttttctt
     6781 ctataaaata gataaaaatg gtacaataat aaattgaggt aataaggatg agattagata
     6841 aatatttaaa agtatcgcga attatcaagc gtcgtacagt cgcaaaggaa gtagcagata
     6901 aaggtagaat caaggttaat ggaatcttgg ccaaaagttc aacggacttg aaagttaatg
     6961 accaagttga aattcgcttt ggcaataagt tgctgcttgt aaaagtacta gagatgaaag
     7021 atagtacaaa aaaagaagat gcagcaggaa tgtatgaaat tatcagtgaa acacgggtag
     7081 aagaaaatgt ctaaaaatat tgtacaattg aataattctt ttattcaaaa tgaataccaa
     7141 cgtcgtcgct acctgatgaa agaacgacaa aaacggaatc gttttatggg aggggtattg
     7201 attttgatta tgctattatt tatcttgcca acttttaatt tagcgcagag ttatcagcaa
     7261 ttactccaaa gacgtcagca attagcagac ttgcaaactc agtatcaaac tttgagtgat
     7321 gaaaaggata aggagacagc atttgctacc aagttgaaag atgaagatta tgctgctaaa
     7381 tatacacgag cgaagtacta ttattctaag tcgagggaaa aagtttatac gattcctgac
     7441 ttgcttcaaa ggtgataaaa tggaaaattt attagacgta atagagcaat ttttgagttt
     7501 gtcagatgaa aagctggaag aattggctga taaaaatcaa ttattgcgtt tacaagaaga
     7561 aaaggaaagg aagaatgcgt aaattcttaa ttattttgtt gctaccaagt tttttgacca
     7621 tttcaaaagt cgttagcaca gaaaaagaag tcgtctatac ttcgaaagaa atttattacc
     7681 tttcacaatc tgactttggt atttatttta gagaaaaatt aagttctccc atggtttatg
     7741 gagaggttcc tgtttatgcg aatgaagatt tagtagtgga atctgggaaa ttgactccca
     7801 aaacaagttt tcaaataacc gagtggcgct taaataaaca aggaattcca gtatttaagc
     7861 tatcaaatca tcaatttata gctgcggaca aacgattttt atatgatcaa tcagaggtaa
     7921 ctccaacaat aaaaaaagta tggttagaat ctgactttaa actgtacaat agtccttatg
     7981 atttaaaaga agtgaaatca tccttatcag cttattcgca agtatcaatc gacaagacca
     8041 tgtttgtaga aggaagagaa tttctacata ttgatcaggc tggatgggta gctaaagaat
     8101 caacttctga agaagataat cggatgagta aagttcaaga aatgttatct gaaaaatatc
     8161 agaaagattc tttctctatt tatgttaagc aactgactac tggaaaagaa gctggtatca
     8221 atcaatatga aaagatgtat gcagccagcg ttttgaaact ctcctatctc tattatacgc
     8281 aagaaaaaat aaatgagggt ctttatcagt tagatacgac tgtaaaatac gtatctgcag
     8341 tcaatgattt tccaggttct tataaaccag agggaagtgg tagtcttcct aaaaaagaag
     8401 ataataaaga atattcttta aaggatttaa ttacgaaagt atcaaaagaa tctgataatg
     8461 tagctcataa tctattggga tattacattt caaaccaatc tgatgccaca ttcaaatcca
     8521 agatgtctgc cattatggga gatgattggg atccaaaaga aaaattgatt tcttctaaga
     8581 tggccgggaa gtttatggaa gctatttata atcaaaatgg atttgtgcta gagtctttga
     8641 ctaaaacaga ttttgatagt cagcgaattg ccaaaggtgt ttctgttaaa gtagctcata
     8701 aaattggaga tgcggatgaa tttaagcatg atacgggtgt tgtctatgca gattctccat
     8761 ttattctttc tattttcact aagaattctg attatgatac gatttctaag atagccaagg
     8821 atgtttatga ggttctaaaa tgagggaacc agatttttta aatcattttc tcaagaaggg
     8881 atatttcaaa aagcatgcta aggcggttct agctctttct ggtggattag attctatgtt
     8941 tctatttaag gtattgtcta cttatcaaaa agagttagag attgaattga ttctggctca
     9001 tgtgaatcat aagcagagaa ttgaatcaga ttgggaagaa aaggaattaa ggaagttggc
     9061 tgctgaagca gagcttccta tttatatcag caatttttca ggagaatttt cagaagcgcg
     9121 tgcacgaaat tttcgttatg atttttttca agaggttatg aaaaagacag gtgcgacagc
     9181 tttagtcact gcccaccatg ctgatgatca ggtggaaacg attttgatgc gcttgattcg
     9241 aggaactcgc ttgcgctatc tatcaggaat taaggagaag caagtagtcg gagagataga
     9301 aatcattcgt cccttcttgc attttcagaa aaaagacttt ccatcaattt ttcactttga
     9361 agatacatca aatcaggaga atcattattt tcgaaatcgt attcgaaact cttacttacc
     9421 agaattggaa aaagaaaatc ctcgatttag ggatgcaatc ttaggcattg gcaatgaaat
     9481 tttagattat gatttggcaa tagctgaatt atctaacaat attaatgtgg aagatttaca
     9541 gcagttattt tcttactctg agtctacaca aagagtttta cttcaaactt atctgaatcg
     9601 ttttccagat ttgaatctta caaaagctca gtttgctgaa gttcagcaga ttttaaaatc
     9661 taaaagccag tatcgtcatc cgattaaaaa tggctatgaa ttgataaaag agtaccaaca
     9721 gtttcagatt tgtaaaatca gtccgcaggc tgatgaaaag gaagatgaac ttgtgttaca
     9781 ctatcaaaat caggtagctt atcaaggata tttattttcc tttggacttc cattagaagg
     9841 tgaattaatt caacaaatac ctgtttcacg tgaaacatcc atacacattc gtcatcgaaa
     9901 aacaggagat gttttgattc aaaatgggca tagaaaaaaa ctcagacgtt tatttattga
     9961 tttgaaaatc cctatggaaa agagaaactc tgctcttatt attgagcaat ttggtgaaat
    10021 tgtctcaatt ttgggaattg cgaccaataa tttgagtaaa aaaacgaaaa atgatataat
    10081 gaacactgta ctttatatag aaaaaataga taggtaaaaa atgttagaaa acgatattaa
    10141 aaaagtcctc gtttcacacg atgaaattac agaagcagct aaaaaactag gtgctcaatt
    10201 aactaaagac tatgcaggaa aaaatccaat cttagttggg attttaaaag gatctattcc
    10261 ttttatggct gaattggtca aacatattga tacacatatt gaaatggact tcatgatggt
    10321 ttctagctac catggtggaa cagcaagtag tggtgttatc aatattaaac aagatgtgac
    10381 tcaagatatc aaaggaagac atgttctatt tgtagaagat atcattgata caggtcaaac
    10441 tttgaagaat ttgcgagata tgtttaaaga aagagaagca gcttctgtta aaattgcaac
    10501 cttgttggat aaaccagaag gacgtgttgt agaaattgag gcagactata cctgctttac
    10561 tatcccaaat gagtttgtag taggttatgg tttagactac aaagaaaatt atcgtaatct
    10621 tccttatatt ggagtattga aagaggaagt gtattcaaat tagaaagaat aatctttaat
    10681 gaaaaaacaa aataatggtt taattaaaaa tccttttcta tggttattat ttatcttttt
    10741 ccttgtgaca ggattccagt atttctattc tgggaataac tcaggaggaa gtcagcaaat
    10801 caactatact gagttggtac aagaaattac cgatggtaat gtaaaagaat taacttacca
    10861 accaaatggt agtgttatcg aagtttctgg tgtctataaa aatcctaaaa caagtaaaga
    10921 aggaacaggt attcagtttt tcacgccatc tgttactaag gtagagaaat ttaccagcac
    10981 tattcttcct gcagatacta ccgtatcaga attgcaaaaa cttgctactg accataaagc
    11041 agaagtaact gttaagcatg aaagttcaag tggtatatgg attaatctac tcgtatccat
    11101 tgtgccattt ggaattctat tcttcttcct attctctatg atgggaaata tgggaggagg
    11161 caatggccgt aatccaatga gttttggacg tagtaaggct aaagcagcaa ataaagaaga
    11221 tattaaagta agattttcag atgttgctgg agctgaagaa gaaaaacaag aactagttga
    11281 agttgttgag ttcttaaaag atccaaaacg attcacaaaa cttggagccc gtattccagc
    11341 aggtgttctt ttggagggac ctccggggac aggtaaaact ttgcttgcta aggcagtcgc
    11401 tggagaagca ggtgttccat tctttagtat ctcaggttct gactttgtag aaatgtttgt
    11461 cggagttgga gctagtcgtg ttcgctctct ttttgaggat gccaaaaaag cagcaccagc
    11521 tatcatcttt atcgatgaaa ttgatgctgt tggacgtcaa cgtggagtcg gtctcggcgg
    11581 aggtaatgac gaacgtgaac aaaccttgaa ccaacttttg attgagatgg atggttttga
    11641 gggaaatgaa gggattatcg tcatcgctgc gacaaaccgt tcagatgtac ttgaccctgc
    11701 ccttttgcgt ccaggacgtt ttgatagaaa agtattggtt ggtcgtcctg atgttaaagg
    11761 tcgtgaagca atcttgaaag ttcacgctaa gaataagcct ttagcagaag atgttgattt
    11821 gaaattagtg gctcaacaaa ctccaggctt tgttggtgct gatttagaga atgtcttgaa
    11881 tgaagcagct ttagttgctg ctcgtcgcaa taaatcgata attgatgctt cagatattga
    11941 tgaagcagaa gatagagtta ttgctggacc ttctaagaaa gataagacag tttcacaaaa
    12001 agaacgagaa ttggttgctt accatgaggc aggacatacc attgttggtc tagtcttgtc
    12061 gaatgctcgc gttgtccata aggttacaat tgtaccacgc ggccgtgcag gcggatacat
    12121 gattgcactt cctaaagagg atcaaatgct tctatctaaa gaagatatga aagagcaatt
    12181 ggctggctta atgggtggac gtgtagctga agaaattatc tttaatgtcc aaaccacagg
    12241 agcttcaaac gactttgaac aagcgacaca aatggcacgt gcaatggtta cagagtacgg
    12301 tatgagtgaa aaacttggcc cagtacaata tgaaggaaac catgctatgc ttggtgcaca
    12361 gagtcctcaa aaatcaattt cagaacaaac agcttatgaa attgatgaag aggttcgttc
    12421 attattaaat gaggcacgaa ataaagctgc tgaaattatt cagtcaaatc gtgaaactca
    12481 caagttaatt gcagaagcat tattgaaata cgaaacattg gatagtacac aaattaaagc
    12541 tctttacgaa acaggaaaga tgcctgaagc agtagaagag gaatctcatg cactatccta
    12601 tgatgaagta aagtcaaaaa tgaatgacga aaaataaccc tgagagaggc tggagcctct
    12661 cttttttgtg cagtttagga gctaaaggga acagaatgga gaaaatggaa caaa
//
LOCUS       DBERDK010000051        12581 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.051, whole genome shotgun sequence.
ACCESSION   DBERDK010000051 DBERDK010000000
VERSION     DBERDK010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 12581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12581
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.051"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..183
                     /locus_tag="ACL73L_001486"
     CDS             <1..183
                     /locus_tag="ACL73L_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGS0772447.1"
                     /translation="KIVNALQLPYSNAKLEATNNLIKLIKRNAFGFRNFENFKKRIFI
                     ALNIKKERTNFVLYRA"
     gene            complement(268..1470)
                     /gene="rpsA"
                     /locus_tag="ACL73L_001487"
     CDS             complement(268..1470)
                     /gene="rpsA"
                     /locus_tag="ACL73L_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HGS0772448.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFVDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSLKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPAQEEGQKEEKRAARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
     gene            complement(1632..1703)
                     /locus_tag="ACL73L_001488"
     tRNA            complement(1632..1703)
                     /locus_tag="ACL73L_001488"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1669..1671),aa:Gln,seq:ttg)
     gene            complement(1713..1793)
                     /locus_tag="ACL73L_001489"
     tRNA            complement(1713..1793)
                     /locus_tag="ACL73L_001489"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1757..1759),aa:Tyr,seq:gta)
     gene            complement(1843..2073)
                     /locus_tag="ACL73L_001490"
     CDS             complement(1843..2073)
                     /locus_tag="ACL73L_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HGS0772449.1"
                     /translation="MSKKDKKIEIQVADAKVNVGKDSFEGYTLTIGKKVIGEIAELDG
                     QFAIIKNGNVDSFYKKLEKAVEILIENYNLAK"
     gene            complement(2241..2885)
                     /gene="pcp"
                     /locus_tag="ACL73L_001491"
     CDS             complement(2241..2885)
                     /gene="pcp"
                     /locus_tag="ACL73L_001491"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HGS0772450.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYFSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIEAIVDYKDKDLQLVGGETH"
     gene            complement(2900..3823)
                     /locus_tag="ACL73L_001492"
     CDS             complement(2900..3823)
                     /locus_tag="ACL73L_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0859 family membrane protein"
                     /protein_id="HGS0772451.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMPNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPQLLGALGAIFTVAGVGDVISHLISGIVPSDSRFIGVLAYVLGMVLFTMIMG
                     NAFAAFTVITAGVGVPFVFALGANPIVAGALAMTAGYCGTLLTPMAANFNALPAALMD
                     MKDQNGVIKAQAGVALVMIVIHIFLMYFLAF"
     gene            complement(3820..4509)
                     /locus_tag="ACL73L_001493"
     CDS             complement(3820..4509)
                     /locus_tag="ACL73L_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0858 family membrane protein"
                     /protein_id="HGS0772452.1"
                     /translation="MEWIRLIGIAIIVVGFILKFDTIATVVLAGLVTALVSGVSLVEF
                     LEILGKEFSNQRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFI
                     RELAGFFSIRLGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGN
                     FFAQNTFVGAGGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKL
                     ISKKTRGGEQK"
     gene            complement(4574..5278)
                     /locus_tag="ACL73L_001494"
     CDS             complement(4574..5278)
                     /locus_tag="ACL73L_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HGS0772453.1"
                     /translation="MVLSTSAILVACGKTDKEADAPTTFSYVYAVDPASLGYSIATRT
                     STTDVIGNVIDGLMENDKYGNVAPSQKDYDLNSTGWAPSYQDPASYLNIMDPKSGSAM
                     KHLGITKGKDKDVVAKPGLDKYKKLLEDAVSETTDLEKRYEKYAKAQAWSTDSSLLMP
                     TASSGGSPVVSNVVPFSKPYSQVGIKGDPYIFKGMKLQKDIVTTKEYNEVFKKWQKEK
                     LESNSKYQKELEKHIK"
     gene            complement(5334..6356)
                     /locus_tag="ACL73L_001495"
     CDS             complement(5334..6356)
                     /locus_tag="ACL73L_001495"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HGS0772454.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVVRANEEYVPPYGTGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEG
                     WIVKVD"
     gene            complement(6488..8959)
                     /gene="parC"
                     /locus_tag="ACL73L_001496"
     CDS             complement(6488..8959)
                     /gene="parC"
                     /locus_tag="ACL73L_001496"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HGS0772455.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKNTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDAAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVITEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQVGIVPILSSYIAHRREVILARSRFDKEKAEKRLHIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFTEEQAEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLA
                     AIIGDERTMYNLMKKELREVKKKFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEEILYVEVLDQFDDATTYFAVTRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILATSRAKRGLQVLRELKN
                     KPHRVFLAGAVAEQGFVGDFFSTEVDVNDQTLLVQSNKGTIYESRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLQEVVTEDK"
     gene            complement(9160..9364)
                     /locus_tag="ACL73L_001497"
                     /pseudo
     CDS             complement(9160..9364)
                     /locus_tag="ACL73L_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014166.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
     gene            complement(9379..11322)
                     /gene="parE"
                     /locus_tag="ACL73L_001498"
     CDS             complement(9379..11322)
                     /gene="parE"
                     /locus_tag="ACL73L_001498"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HGS0772456.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFENGGKPVTTLK
                     KIGTAPKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLTDKRTDE
                     AIEFHYENGVQDFVSYLNEDKEILTPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGLKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLAAVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVINTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRRKWIEDNVKFTLEEATVF"
     gene            11458..12099
                     /gene="plsY"
                     /locus_tag="ACL73L_001499"
     CDS             11458..12099
                     /gene="plsY"
                     /locus_tag="ACL73L_001499"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HGS0772457.1"
                     /translation="MITIVLLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIIFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVIFGFAPIFCLYLAIIFFGALYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILSNYDSLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
     gene            complement(12144..>12581)
                     /locus_tag="ACL73L_001500"
     CDS             complement(12144..>12581)
                     /locus_tag="ACL73L_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGS0772458.1"
                     /translation="QKLYLSPVLDGFNSEIIAYNLSCSPNLEQVQTMLEQAFTEKHYE
                     NTILHSDQGWQYQHDSYHQFLEGKGIQASMSRKGNSPDNGMMESFFGILKSEMFYGYE
                     KTFKSLNQLEQAIIDYIDYYNNKRIKVKLKGLIPVQYRTKSFG"
BASE COUNT         3636 a         2834 c         2209 g         3902 t
ORIGIN      
        1 aaaatcgtca acgctcttca attaccttat tccaacgcaa aattggaagc gaccaataat
       61 ctcatcaaac ttatcaaacg aaacgccttt ggttttcgaa actttgaaaa cttcaaaaaa
      121 cggattttta tcgctctgaa tatcaaaaaa gaaaggacga attttgtcct ttatcgagct
      181 tagctgactt caacccacta cagttgacaa agagccagaa aaatcccttg tttagtaaac
      241 aaaggatttt gtgaattttc aatttgatta aagttcgatg tcaccaaaca aatcagccat
      301 tgaaaatcct gtttgtgttt ctggaagttc gaaatcacgc ttttcttgac gtcttggacg
      361 acgtggacga gcagcacgtt tttcttcttt ttgtccttct tcttgggctg gacgttcttc
      421 aagagcttta atagaaagtg acacgcgttc tgcatctgcg ttaacttcaa gaactttaac
      481 ttgaacttct tgaccaactt taagagcttc ttttggattt tcaatccgtt tgtgtgaaat
      541 ttgtgatacg tgaacaagtc catcgatacc tggcaatact tcaacaaatg caccgaagtc
      601 agtcaaacgt ttaactgttc cttctactac atcaccttta gccaattttt gctcaacgcc
      661 atcccatggt cctggtactg ttgctttaag tgaaagtgat acacgtcctt cttcttcgtt
      721 aagatcaagg attttcactt caatttcttc accaacagtt acaactgatt ttggtgatac
      781 gttacgttca tgtgacaatt cagtcaagtg aaccaatccg tcaacaccac caaggtcgac
      841 gaaagcgccg aagcttgtga tacgagcaac tttaccagtt acaacatcac caacagccaa
      901 tttaccgaat acttcagcgc gagctgctgc agtagctgct tcaacaactt cacgacgtga
      961 aaggatgaag cggttttctt tagcgttaac ttctttgatt ttagtatcaa attcttgacc
     1021 tacaaaacgc tcagcattac gtacgaaacg agtatccaac attgaagctg ggataaatcc
     1081 acgaacacct tcaaattcta ctgaaagtcc acctttaacg gcacgcgttc ctttaacagt
     1141 aacaacttct tcttcgcgac caacaagttt gtcccatgct ttgcgagctt caaggcgttt
     1201 tttagataca aggtatgtaa ctgtatcagt atctttacca actacttgac gaagtacaag
     1261 aacatccaat acttctccta ctttaacaaa gtcattgata tctgcatcac gatcgtttgt
     1321 caattcgcga agagtcaaga caccttcaac accagttcca gagattgcaa cgttagcttg
     1381 agtcgcatca actgtcaata cttcagcact aacaacatca ccagtctcaa cttgactaac
     1441 gctatttagc aaatcttcaa attcgttcat ctaaaaaatc ctccaacaat caagtttttc
     1501 ttaaacttga catacttata atttttttcc taagcacccg caaggacatg ttcatatcat
     1561 tcacgtcttt caattataaa aataaaatac cctaagatat tttgcttttt atcttgatta
     1621 ttacctaaga actggggtag ctggattcga accaacgcat gagggagtca aagtcccttg
     1681 ccttaccgct tggctatacc ccattaatga aatggagaga gagggattcg aacccccgaa
     1741 cccgaaggag cggatttaca gtccgccgcg tttagcctct tcgctatctc tcctacaatc
     1801 aacatggact attatatcat gaaaatttca aaaaaacaag acttattttg ctaaattata
     1861 attttcaatc aaaatttcca cagctttttc caatttttta taaaaactat cgacattccc
     1921 attctttata atggcaaatt gtccgtctaa ttcggcaatt tctccgataa cttttttacc
     1981 gatagtcaat gtataacctt caaaactgtc tttaccaaca ttaactttgg catccgctac
     2041 ttgaatttcg atttttttat ctttcttgct catttcatac ctctcttcac tttttctatt
     2101 ttacaagaaa attctaaaac tagcaagaaa aaactctata tcaggcttct ctgatataga
     2161 gttttatgta aacaacctca attgatttag catagttaat agttagtaaa caagcataat
     2221 tgactatcct ttttaggtta tcagtgagtc tcaccgccta ctaactgcaa atccttatct
     2281 ttgtagtcta caatagcttc aatagctacc tctatgcctc ttacaatatc acatagactc
     2341 attcctgcag tattcggctt atttactact tgttccatca tatatggaat atgcatgaaa
     2401 cctgctctca tattagggaa ttttttatcc actaaataaa gagcttggta catcaaatga
     2461 ttgcaaacaa aggttcctgc actattggat acaactgccg gaagtccctg ttttttgata
     2521 gcttgtacca tcgctttgat aggtaaacta ctaaaatagg ccgatgctcc atcaatacga
     2581 atcggtgtat caattggttg attgccttcg ttatcaggta tgcgagcatc atcttgatta
     2641 atagccactc gttcaggtgt taagccggtc ctgccgcctg cttgtccaat acaaagtaca
     2701 gcatctggtt gatatcgtaa tatttctgcc tctaaaactt ctgacgactt ataaaaaacc
     2761 gttggaattt ctacccagcg aacttcagcc ccattaatct cagatggtaa taattttaca
     2821 gcctccaaag ctggattaat cttttcacct ccaaaaggat taaaacctgt aaccaatatt
     2881 ttcattttat tttcctttac taaaatgcga gaaagtacat taagaatatg tgaataacaa
     2941 tcattactag agcaacacct gcttgagcct ttataacgcc attctgatct ttcatatcca
     3001 tcaatgctgc tggtagagcg ttaaaattag cagccattgg ggtcaataaa gtcccacaat
     3061 aacctgctgt catggcaaga gcaccagcca caattggatt agctcccaga gcaaatacaa
     3121 agggaactcc aacacctgct gtaataacgg tgaatgctgc aaaagcattt cccataatca
     3181 ttgtgaatag aaccattcca agaacatagg ccaaaactcc tataaagcga ctatctgaag
     3241 gaacaatacc gctaatcaga tgagagataa catcaccaac acctgctaca gtaaaaatag
     3301 cccccaaagc ccctaataat tgaggaacaa tcccacttgt tgaaacttgc tgagtcattc
     3361 gattattttc tgataacaga ctcttagggt gactattggt aatcacaaga acagaaattg
     3421 tagcaaacaa ggcggcaagg ctaatcgaaa tcttgctaaa ttctggaatc atttgcgcta
     3481 agaccaacgc aagtattgcc atcagcataa ctggaataaa aattttattt ttcaacctgt
     3541 tagattcaat attggctttc atttcatcta aggatggcaa ggttccgata cggacttgct
     3601 taaacaatgt taacagcgat aataggatta caataatacc aatactcata tttggcatat
     3661 aggaaccacc tataaacgta atagacaata gagtccaaaa tgcagatgtc ccaagtcgaa
     3721 ctgggtttgt tttatcttta taactacaat aggctgtatg gagaaattga caaccaatca
     3781 caatataggt caactctaat agttgctttg ccaactctgt catttttgtt ctcctcccct
     3841 agtctttttt gatatcaatt ttttatcaaa taaataattg taaatcccca ctacaataag
     3901 tgttataaca gcaacaataa tagatgtaga agcaatccct gcataattgc tttcatagcc
     3961 taactgatct aatgttcccc ctatcaagag gactccccca gcacctacaa acgtattttg
     4021 agcaaagaaa tttccaaaat tttcattcgc agccgcacgc gcttttattg tctcatcttc
     4081 aacctctgtt aactttctac ctaattgaga ctctgcagct gcttctccca taggttgaac
     4141 caaaggtctg acaaactgag ggtgtcctcc tagacgaatt gaaaagaaac cagctaactc
     4201 tcgaataaag aaataaactg tatagaagtt tccaactgtc agacctttaa tctttcgaat
     4261 caaatcgatt gatcgttgct tgagtccaaa ggtttctgac agccccacaa gaggcaaggt
     4321 aaccataaaa atcgtgagca ctcgctgatt gctaaattct tttcccaaaa tctccaaaaa
     4381 ttcaacgaga gaaacacctg aaactaaagc tgtaaccaaa ccagctaaga ctactgttgc
     4441 aattgtatca aattttaaaa taaaacccac aacaatgatt gctattccta ttaatctaat
     4501 ccactccata tcaaactcct ttatattcaa aatgacagta tttttaaaat tttatcaaga
     4561 tcaataccat tccttattta atgtgttttt ctagttcttt ttggtatttg ctattggatt
     4621 ccaatttttc tttttgccat tttttaaaaa cctcgttata ttcttttgtt gtaacaatat
     4681 ctttttgcaa tttcattcct ttaaagatat atggatcccc cttaatacca acttgtgagt
     4741 atggttttga gaatggtact acgttactta caactggaga accaccagat gaagctgttg
     4801 gcatcaataa tgaactatct gtcgaccaag cttgagcttt ggcatatttt tcatatctct
     4861 tctctaggtc agtggtctca gaaacagcat cttctaacaa tttcttatat ttatccaaac
     4921 caggtttagc tacaacatcc ttatcttttc ctttcgtaat accaaggtgt ttcatggcag
     4981 aaccagattt tggatccata atattcaagt aagacgctgg atcttgatag cttggagccc
     5041 atcctgtact attcaaatca tagtcttttt gagaaggagc aacattgccg tatttatcat
     5101 tttccatcaa accatcaata acatttccaa taacgtctgt cgtcgatgtt cgagtcgcta
     5161 tactgtagcc caatgatgct ggatctactg catagacata agaaaatgtt gtcggtgcat
     5221 ctgcttcttt atcagttttt ccacaagcca ctaaaatagc tgacgtgctc aggaccactc
     5281 ctgctgttaa gagccacttt ttctatttca taaaaaatct cctttggttt attttaatct
     5341 acttttacaa tccaaccttc tggcgcttca atatcgccaa actgaatacc cgtcaattca
     5401 ttatataatt tacgcgtcac aggacctact tctgtttcac tatagaatac atggaaatca
     5461 tcaccatgtt gaatacctcc aattggagaa ataaccgctg ctgtaccaca ggcacctgcc
     5521 tctacaaaac ggtcaagatt atcaattgga acatcaccct caataggagt taatcccaag
     5581 cgatgttctg ccaaataaag caaggaatac ttggtaatag atggcaagat agatggactc
     5641 aatggtgtta caaattcatt atcagctgta attccaaaga agttagctga tccgacttct
     5701 tcaatctttg tatgagttga tgggtccaga tagataacat ctgagaaatg acgtgacttg
     5761 gccatttttc ctggtaagag acttgcagca tagtttccac caaccttagc cgcacctgta
     5821 ccatttggtg ctgcacggtc gtactcatcc tgaatcaaga agttggttgg gaccaaacca
     5881 cctttaaagt aatttccaac tggcatagca aagatggtga aaatgtactc ttctgccggt
     5941 tttaccccga taatatctcc gacaccaatc aaaagagggc gaagatataa ggttccacct
     6001 gttccgtatg gtggtacgta ttcttcattc gcacggacaa ctgctttaca agcttctaca
     6061 aacatgtctg tcggaacttg tggcatcaag agacggtcac atgtacgttg cagacgttta
     6121 gcattttcat caggacggaa cagttgaaca ctgccatcct tagtacgata agctttcaaa
     6181 ccttcaaatg cttgttgtcc atagtgaaga cttggagaag actctgaaat atgcaaagtt
     6241 gcatcctctg taagctctcc ttgatcccat tgtccatttt tgaaatgagc aagatagcga
     6301 taaggtaatt tcatatagga aaaaccgagg ttttcccaat caatcgttac tgtcatattc
     6361 cactccttat tctaaaaacc tatttcttat attctacact atttttctaa aatagcaagt
     6421 atattttgta attttcagaa aatttctcca ataaaaacca gctcttagaa ctgattcttc
     6481 atttcactta tttatcttca gtaactactt cctgaagata agcgtcaaaa acttcttcat
     6541 ctgaaatcgt gtcagaaatg aagcttccat tgctagtgcg ttctgacaag ttcaagtctt
     6601 gcaatcggct ttcatagatt gttcctttat tggattggac aagcagagtt tggtcgttca
     6661 catccacttc cgtactgaag aaatcgccaa caaatccttg ctctgcaact gctcctgcca
     6721 agaagacacg atgcggtttg tttttcaact cacgcaagac ttgtaatcct cgtttggcac
     6781 ggctggttgc tagaatttcc tcaatagaaa cacgtttcaa gcttccacgc tgggtcaaga
     6841 ggtagaagga cgaagtatta cagataaagc cagattggag gacatcatct tctttcaaat
     6901 tcatagcctt gacacctgct gccttagcac cgacaaccgg aacctcttcg atattgaaac
     6961 gcagggcata accattttga ctaaccaaga caacatcatc tagtttaatc ggagccactg
     7021 ctacaatctg atctgtatcg tctttgagct tagcatactt gacagactta gatctatagg
     7081 tccgccatgg agtgaattct tttcgctcta cccgtttgat ttgaccaagg cgagtcactg
     7141 caaagtaggt tgtcgcatcg tcaaactgat ccagtacttc cacataaagg atttcttcat
     7201 tcgtttcaaa gtttgtgatg gtttggctca gatgctctcc gatgtccttc caacgaatat
     7261 ctgccaactc atggattggt ctgtagatga catttccaag acttgtgaac atcaagaggt
     7321 gctgggttgt cttggcagat tgaacaaaaa tcaaacggtc atcatcacgc ttgccaattt
     7381 cttccaaggt ggaagccgca aaggaacgtg gactggtacg cttgatgtaa cctgccttgg
     7441 tcacgctgac gtaggtatct tcctcagcga taagactagc tgtatcaatc tcaattgctt
     7501 tcgcagtgtc ttctaaagaa ctcaaacgag gagttgcaaa tttcttcttg acctcacgaa
     7561 gttctttctt catgagattg tacatagtcc tttcatcacc gataatagcc gccagcatag
     7621 caatcttctc acgaagctct gcttcttctt cctgcaagac aaccacatcg gtattggtca
     7681 aacggtacag ttgcaaagtt acgatagcct cagcctgttc ttccgtaaaa tcatagctaa
     7741 ctttgaggtt ttccttggcg tccgccttat tctcagaagc acggataaga gcaatgactt
     7801 catccaaaat cgaaatcaca cgaatcaaac cttcgacgat atggagacgt ttctcagcct
     7861 tttctttgtc aaagcgtgaa cgcgccaaaa tcacttctcg acggtgagcg atgtagctag
     7921 acaggattgg aacaatccca acctgacgag gtgtgaaatt gtcaatcgcc accatattaa
     7981 agttgtagtt gatttgtagg tcggtgtact taaataagta gttgagaaca agctcagtat
     8041 tagcgtcttt cttaagttcg atagcgatac gaagaccatc acggtcagac tcatcacgaa
     8101 cctcagcaat cccagctacc ttgttattaa cacgaacatc atcgattttc ttgactagat
     8161 tggccttatt gatttcataa ggaatctcag taataacgat ttgttcctta ccacctttta
     8221 gcttttcaat ttcagtcttg gaacgaacaa ccacgcgccc tttcccagtc tcataagctt
     8281 tcttgatttc atcacgaccc tgaataatag cccctgtagg gaagtctggt ccaggcaaga
     8341 attccatgag tttatcaatc tttgcagttg ggtggtcaat catgtaaact gcagcatcta
     8401 tgacctcagc taaattatgg ggaggaatgt ctgtggcata accagccgaa atcccagtcg
     8461 aaccattgac caagaggttt ggaaaggctg ctggcaagac cgttggttct ttctccgtat
     8521 cgtcaaagtt ccatgcaaaa ggaactgtat ttttctcgat atcctgaaga aggtaacctg
     8581 caatttcaga caaacgtgcc tcagtataac gcatagccgc aggaggatct ccgtccatag
     8641 aaccgttatt accgtgcatt tcaactagaa tctcacgatt tttccagttc tgtgacatac
     8701 gaaccatggc atcatagata gaagaatccc cgtgtgggtg gaaattcccc atgatgttcc
     8761 cgactgactt ggccgactta cggtagctct tgtcaaaagt attgctatcc ttattcatag
     8821 aataaagaat acggcgctga accggcttca acccatcacg aatatctggc aaagcccggt
     8881 cttgaataat gtacttggag tagcgaccaa agcgctctcc catgatgtcc tccagggaca
     8941 tgttttgaat gttagacata agatacaaag cccataaaat accaagtgaa aatagaaaat
     9001 tcttgaagta agcaaactca caagagaatt tatctttttc acacagtatc tagggcgtgt
     9061 tcaactcctt tcaaagaatg tagagtaggt ttttatgcag taaaagatat tttacgggaa
     9121 ttcctcccgt gttcagttac gataagtaac caaactatcc tgtttgtatt tttcaatatg
     9181 aaaatctggt tttccaaaat tagtcttagt ttgtgtctta accgctccct taagcgcctc
     9241 tttgagataa gcactcatag cagattcttc attaataatc ctgcaatttt ttcaaaccaa
     9301 gattttcaaa ctgctttttc acatagtcat tcacatccga ctctaatttc cagtttacta
     9361 acatattatt ttctttcatt aaaacactgt cgcttcttct agcgtaaact tgacattatc
     9421 ttcaatccat ttacggcgtg gttctacctt atctcccatg agaacattga cgcggcgttc
     9481 ggcgcgcgct aaatcttcaa ttgtgacacg gatgagggta cgtgtttctg ggttcatggt
     9541 tgtttcccag agctggtccg cattcatctc accaagtcct ttgtatcgtt ggagggtagc
     9601 gcctttaccg aactgtttac ggagttcttc tagttctccg tccgtccaag cgtaggccac
     9661 ttcttctttc ttgcctttac ctttggacat cttgtaaaga ggtgggaggg caatatagac
     9721 atgacctgcc tcgactagcg gacgcatgta acggtagaaa aatgtcaaga gcaaggtctg
     9781 gatatgggca ccgtcggtat ccgcatcggt catgataatg atcttatcat agttggcatc
     9841 ttcaatagag aagtctgctc caacacccgc accaatggta taaatcatgg tattgatctc
     9901 ttcatttttg aggatatccg ccatcttggc cttggctgta ttgataacct taccacgaag
     9961 aggtagaata gcctggaact tgcggtcacg accttgtttg gcagaaccac cggcagagtc
    10021 cccctcaact agatagagtt cattcttagc aggattctta gattgggctg gggtcaattt
    10081 cccagacaac aagcccttat ctttcttgtt tttcttccca tttcggctct catcacgcgc
    10141 cttacgtgct gcttcacgag catcacgggc cttgatagcc ttgcggatga ggttagaagc
    10201 taattcccca ttttccataa ggaaaaaggt caacttatca gccactattc catccacaac
    10261 tgggcgagct agggggcttc ctagtttatc cttggtctgt ccttcaaact gcaagtgttc
    10321 ttcaggaact aagatagaaa gaacggccgc tagtccctca cgatagtctg aaccttcaag
    10381 gtttttatct ttttccttga gaagacctgt tttacgtgca tagtcattca tgaccttggt
    10441 aatggcagac ttgagtcctg tctcgtgcgt tccaccgtcc ttggtgcgaa cgttattgac
    10501 aaaggataga atgttatctg agaatccgtc attgtactgg agggctactt ccacttgaaa
    10561 accattgtct tccccttcaa agtaaagaac tggcgtcaag atttccttat cttcgttgag
    10621 ataagaaaca aaatcttgta ctccattctc atagtggaac tcaatcgctt catctgttcg
    10681 cttgtccgtt aaagacaagg tcacattttt caagagaaag gctgattcat taaggcgctc
    10741 tgaaatggta ttgtacttga aatctgtcgt agaaaagata gtcgcgtcag gcataaaagt
    10801 aactttggtg cctgttttag acttgggtgc tgtaccgatt ttcttcaaag tcgtgacagg
    10861 ttttccacca ttttcgaaac gttgcttgta aactgcgcca tcacgggtaa tttcaacttc
    10921 taaccagcta gaaagggcgt taacaacgga agaacccact ccgtgaagtc cacctgatgt
    10981 cttatagcca ccttgaccga atttccctcc ggcatgaaga atggtaaaga taacctcaac
    11041 agttggaatt cccatagcgt gcatacctgt cggcatccca cgtccatggt cttgaaccgt
    11101 tagactaccg tctttattga tggttacatc gatacgatca ccaaacccag acaaggcttc
    11161 atcgactgca ttatcaacga tttcccaaac taggtgatga agaccagcgc catcggtcga
    11221 tccaatatac atccctggac gttttcggac cgcatccaac ccttctagca cctgaatagc
    11281 atcatcatta taattgttaa tattgatttc cttttttgac acaaggaacc tcctattcgt
    11341 tcatctttac tattctacag gttttccaag gattttgcaa aatttttctt tctccgatgt
    11401 gacaatttta gcagagattc tctgcttttc tttcccaatt catgatataa taggagtatg
    11461 attacaatag ttttattaat cctagcctat ctgctgggtt cgattccatc tggtctctgg
    11521 attggacaag tattctttca aatcaatcta cgcgagcatg gttctggtaa cactggaacg
    11581 accaatacct tccgcatttt aggtaagaaa gctggcatgg caacctttgt gattgacttt
    11641 ttcaaaggaa ccctagcaac gctgcttccg attatttttc atctacaagg cgtttctcct
    11701 ctcatctttg gacttttggc tgttatcggc cataccttcc ctatctttgc aggatttaaa
    11761 ggtggtaagg ctgtcgcaac cagtgctgga gtaattttcg gatttgcgcc tatcttctgt
    11821 ctctaccttg cgattatctt ctttggagct ctctatcttg gcagtatgat ttcactgtct
    11881 agtgtcacag catcgatcgc ggctgttatc ggggttctgc tctttccact ttttggtttt
    11941 atcctgagta actatgactc tctcttcatc gctattatct tagcacttgc tagtttgatt
    12001 atcattcgtc ataaggacaa tatagctcgt atcaaaaata aaactgaaaa tttggtccct
    12061 tggggattga acttaaccca tcaagatcct aaaaaataaa atgccagttc tgtgaactgc
    12121 accccaaaag ttagacaatt aatttatcca aaggatttag ttctgtattg cacagggata
    12181 agtcctttta gttttacctt aattcgttta ttgttgtagt aatcaatata gtctataata
    12241 gcttgttcca attgattaag tgatttaaat gttttctcat aaccgtaaaa catttccgat
    12301 ttcagaatcc caaagaagga ctccatcata ccattgtctg ggctgttacc cttacgtgac
    12361 atggatgctt gaattccctt accctcaaga aactgatgat aagaatcgtg ttgatattgc
    12421 cagccttggt cactatggag aatcgtattc tcgtagtgct tctctgtgaa tgcctgttcc
    12481 aacattgttt gtacttgttc taaattaggc gaacaagaaa gattataggc gataatttcg
    12541 ctattaaagc catctaaaac tggtgataag taaagctttt g
//
LOCUS       DBERDK010000052        12433 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.052, whole genome shotgun sequence.
ACCESSION   DBERDK010000052 DBERDK010000000
VERSION     DBERDK010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 12433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12433
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.052"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(121..468)
                     /locus_tag="ACL73L_001501"
     CDS             complement(121..468)
                     /locus_tag="ACL73L_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGS0772459.1"
                     /translation="MNLNQLDIIVSNVPQVCADLEHILDKKADYADDGFAQFTIGSHC
                     LMLSQNHLVPLENFQSGIIIHIEVEDVDQNYKRLNELGIKVLHGPTVTDWGTESLLVQ
                     GPAALVLDFYRMK"
     gene            complement(579..2354)
                     /gene="spxB"
                     /locus_tag="ACL73L_001502"
     CDS             complement(579..2354)
                     /gene="spxB"
                     /locus_tag="ACL73L_001502"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HGS0772460.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAVSKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
     gene            complement(2561..4804)
                     /locus_tag="ACL73L_001503"
     CDS             complement(2561..4804)
                     /locus_tag="ACL73L_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HGS0772461.1"
                     /translation="MTEIVKASLENGIQKIRIRSEKGYHPAHIQLQKGIPAEITFHRA
                     TPSNCYKEILFEEEGILEPIGVDEEKVIRFTPQELGRHEFFCGMKMQKGSYTVVEKTR
                     KSLSLLQRFWITSIFTVPLVILMIGMLTGSISHQVMHWGTFLATTPIMLVAGKPYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEVLSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDVIGDEVEVFGLAASLEDASQHPLAEAIVKRASEAGLEFQTVENFQALHGKGV
                     SGRINGKQVLLGNAKMLDGMDISNTYQDKLEELEKEAKTVVFLAVDNEIKGLLALQDI
                     PKKNAKLAISQLKKRGLGTVMLTGDNAGVARAIADQIGIEEVIAGVLPEEKAHEIHKL
                     QQSGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNLLGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLALNPELAGLAMAFSSVSVLTSSLL
                     LNFSKID"
     gene            complement(4814..5185)
                     /locus_tag="ACL73L_001504"
     CDS             complement(4814..5185)
                     /locus_tag="ACL73L_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HGS0772462.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKNPEKSGQKAQQKNGYQEIRVE
                     VMGGYTPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHTNLPMGEEYVVEITPE
                     QAGEFGFACGMNMMHGKMIVE"
     gene            complement(5196..5591)
                     /locus_tag="ACL73L_001505"
     CDS             complement(5196..5591)
                     /locus_tag="ACL73L_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HGS0772463.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTVQTLL
                     ARLVEKECLTRKKEGKSFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     regulatory      5775..5873
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5957..6748
                     /gene="thiD"
                     /locus_tag="ACL73L_001506"
     CDS             5957..6748
                     /gene="thiD"
                     /locus_tag="ACL73L_001506"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HGS0772464.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQARDVYGVAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLESVFSDIPPQAVKTGMLATTEIMEIIQPYLKKLDCPY
                     VLDPVMVATSGDALIDSSARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEDMQRA
                     GRLILKEFGPQSVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVI
                     TAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD"
     gene            complement(6958..7590)
                     /gene="thiE"
                     /locus_tag="ACL73L_001507"
     CDS             complement(6958..7590)
                     /gene="thiE"
                     /locus_tag="ACL73L_001507"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HGS0772465.1"
                     /translation="MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARQVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPI
                     VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLTKLHDILS"
     gene            complement(7583..8386)
                     /locus_tag="ACL73L_001508"
     CDS             complement(7583..8386)
                     /locus_tag="ACL73L_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HGS0772466.1"
                     /translation="MQEFTNPFPIGSSSLIHCMTNEISCEMLANGILALGCKPVMADD
                     PREVLDFTKQSQALFINLGHLSAEKEKAIRIAASYAAQVCLPMVVDAVGVTASSIRKS
                     LVRDLLDYRPTVLKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYHG
                     MSFLVTGPKDLVVSKNQVAVLGNGCAELDWITGTGDLVGALTAVFLSQGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSLLRRDENWLDTIKGEVYE"
     gene            complement(8386..8910)
                     /gene="thiW"
                     /locus_tag="ACL73L_001509"
     CDS             complement(8386..8910)
                     /gene="thiW"
                     /locus_tag="ACL73L_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HGS0772467.1"
                     /translation="MRKHQLQVHKLTILSMMTALDVVLTPIFRIEGMAPMSSVVNILA
                     GIMMGPVYALAMATVTAFIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFYFSALGE
                     IVGTGIIGSIVSYPVMVLFTGSAAKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFSERID"
     gene            complement(8915..9607)
                     /gene="tenA"
                     /locus_tag="ACL73L_001510"
     CDS             complement(8915..9607)
                     /gene="tenA"
                     /locus_tag="ACL73L_001510"
                     /EC_number="3.5.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050334 - thiaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HGS0772468.1"
                     /translation="MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKAFSEIYHLLADKTSNQEMKRLLKQNAQGLVEGELFIRQQFFKELEINDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDAFHISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEE"
     gene            complement(9618..10268)
                     /locus_tag="ACL73L_001511"
     CDS             complement(9618..10268)
                     /locus_tag="ACL73L_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HGS0772469.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFIPSIALNLSVVAFCILFMLY
                     YRRFKMLAWMIILAILPSFANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSISLK
                     ELLLYLAQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQKLHFWSPLIYSKV
                     LMTVFRWRHLYLRALSAHGYDEHAQLKNSYRTFYIPKKTKLIYLLFFLLLQTSLFL"
     gene            complement(10270..11655)
                     /locus_tag="ACL73L_001512"
     CDS             complement(10270..11655)
                     /locus_tag="ACL73L_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGS0772470.1"
                     /translation="MGLELRAIQSPIFSEPFDFTFHAQAFTLLVGSSGSGKSSLFQVI
                     AQVSSLPYSGQVLIDGSEVSQLSIIARVQKVGILFQNPNHQFTMESLFEELIFTLENI
                     GYHLQEIDSKIAEVVQQCRCEAILHRPIHHLSGGEKQKAALAVLFAMNPRVYLLDEPF
                     ASIDRKSRIEILEILKELALDGKTVILCDHDLSDYKAYIDHMVELRDGKLREVFQIPS
                     YEMTQVASKEVASSPELFHMDRTTCEFGNRPLFSIADFTFYQGISCILGDNGVGKSTL
                     FRSILQFQKYKGRIAWKGSVLKKKKSLYRDLTGVVQEAEKQFIRVSLREELQLDGPDS
                     ERNQRIFQALRYFDLEQAVDKSPYQLSGGQQKILQLLTILTSKASVILLDEPFAGLDD
                     RACHYFCKWIVEERNQGRSFLLISHRLDPLISVVDYWIEMTSQGLRHVKEVTITKPLT
                     SQSSNTQGEVR"
     gene            complement(11656..12216)
                     /locus_tag="ACL73L_001513"
     CDS             complement(11656..12216)
                     /locus_tag="ACL73L_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HGS0772471.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNILSLLLTPLGLQ
                     AFANEILFGLWCMAAPIAAIFVPRVGSATIGEVLAALAEVLYGSQFGLGALLSGLVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGITLVSFVYEYIKLGYYAFSLPFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
BASE COUNT         3738 a         2762 c         2385 g         3548 t
ORIGIN      
        1 atgagtatta tatgcaattt ctttatataa aaagaccgga ttgctccgat ctttcaatag
       61 ttcatattct caatttctat tttaaaaata gctaaggtta acgtcaaatg actacgcgac
      121 ctatttcata cgataaaaat caagcactag agcagcaggt ccttgaacta ataaggactc
      181 tgttccccaa tcggttacag ttggtccgtg taaaaccttt ataccaagct cgttcaaccg
      241 tttgtagttc tggtctacat cctcaacctc gatatgaata atgattcctg actgaaagtt
      301 ttccaaagga accaaatgat tttgtgacaa cataaggcag tgactaccaa tcgtaaactg
      361 agcaaaacca tcatcagcat aatctgcctt tttatccaag atatgctcca agtcagcaca
      421 gacttgggga acatttgaaa cgataatatc taattgattt aaattcattt actctcctcc
      481 ataaaaagac cggattgctc cgatctttta aagttctgct ctatgaaaat caaagaataa
      541 agtctacaag tttcatattt gattttcggc gagaggaatt atttaattgc gcgtgattgc
      601 aatccttctt cttccaagaa gagacggaat ggtacgagtt cttctgcttc gtatttttcc
      661 ttgaaggctt tgatagcttc ttctgagtga agttttggat ccaattcaag tacttctact
      721 ggaagtggac ggtgttgagt gatgcgagca tcgatgacaa cagttttacc ttctttgttc
      781 aatttaacag cttctgcaac aactgcatcg atgtcttcga tacggtcaac tgtgaatcca
      841 acagctcctt gagcttccgc aattttagcg tagtcagcgt ttgtgaagtc tacaccaaac
      901 aagtgtttgt ttgtatcttc gtatttgttc ttgatgaagc cgtactcagc atttgagaag
      961 acaaggttga taactggaag gtcgtattga acgtttgtga taacgtctgg gtagcacatg
     1021 ttgaatgctc cgtcacccat gatgttccat acttggcgat ctggattgtc tttcttagca
     1081 gcgataccac caggaagggc aatacccatt gtcgcaaaga gtggagatgt acgccacatg
     1141 ttcttaggtg tcatgtgaag gtgacgagta gatgtttgag tagtgttacc tacgtcgatt
     1201 gagtagatag cgtcttgatc agcatgtttg ttgattgcat tgtaaacttg atacaattgc
     1261 aattcaccct cagttttacc ttcgagtttg ttcatgtaat cacgccagtt ttggttgttc
     1321 ttaacgtttg cacgccacca tggagttgat tcaactgggt ttactttgtc aaggatagct
     1381 ttagctgctt gaccagcatc accaaggatt gaagcgtcaa gggcatgacg tttaccaagt
     1441 ttgtaagggt cgatatcgac ttggataaat ttttcagtgt tcttgaatgc ttcgtaaact
     1501 tcagcaaatg ggaagtttga accaaggaaa agaactgtgt ctgcttcaaa gaccacttcg
     1561 ttggctggtt tccaaccaac acggtaagca gaacctgtca aaccttcata gttccattcg
     1621 aaagcttcaa agtttttacc agttgtgatg attggtgctt tgattttacg tgacaattca
     1681 gtaatcactt caccagcttt aacaccacca aatccagcat agataactgg gcgttcagca
     1741 ttgttcaaga tttcaacagc tttgtcgatt tcaacttcgt tcaaagcagg agcgatgaat
     1801 gagcgttcgt atgaacctga accgtagtat gagttttcgt cgatttcttg gaaaccgaag
     1861 tttactggaa tttcaacaac agctggacct tttttagaaa ctgcagcacg gcaggcttcg
     1921 tcaattactt ttggcaattg ctcagcgtaa gctacacgtt tgttgtaaac agcgataccg
     1981 ttgtacattg ggttttggtt aagctcttgg aaagcatcca tgttcaattc gttaactgga
     2041 cgtgatccaa ggattgctag gaatggagtg ttatccatag ctgcatcgta aacaccgtta
     2101 atcaagtgag tcgcacctgg accacctgaa ccaactgcaa ccccgattga gccgccgaat
     2161 ttagcttgca taaccgctgc aagagcacct gtctcttcgt ggcgaacttg taagaagcgg
     2221 atatctttgt cttcagccaa agcgtccatc aatgagctga gtgttcctga tgggataccg
     2281 tagattgtat ctacgcccca tgttttcaat acgttaagca ttgctgcaga tgcagtaatt
     2341 ttcccttgag tcataatgat aactctcctt caattttttt agacttggag aatacgatta
     2401 catagaattg gaaacgttct ccaaattttt actattccac tgtatcatat ttatgctgac
     2461 ttttctaaaa atctgctcaa aactctctat tctctattct aatacagttt tgaaagtgtc
     2521 gtcatttctg ttttataaca aaaaaatcta gtcattactt ttagtctatt ttactaaaat
     2581 ttaacagaag ggaactggtc agaacagata cagaactaaa ggccatggct agacctgcca
     2641 attctgggtt gagagccagt ccaacacctg aaaagactcc tgctgcaatc ggaattccga
     2701 caacattgta gataaaagcc cagaaaagat tgagcagaat acgatgaaag gttttcttac
     2761 tcatgtcgaa ggcacgcacc actcctaaaa gattattggt tgtcaacacc aaatctgctg
     2821 actcgatggc gatatctgtt ccagctccca tagcaatccc cacatctgct acactaaggg
     2881 caggagcgtc attgatccca tcaccaacaa aggctacttt acctgactgt tgcagtttat
     2941 ggatttcatg ggctttttct tctggcaaga cgcctgcaat gacctcttca attccgattt
     3001 gatctgcaat agcacgcgcc acaccagcat tgtctcctgt cagcatgact gtcccgagac
     3061 cacgtttttt tagctgactg atggctagct tagcattttt cttaggaata tcttgcaaag
     3121 caagcaagcc tttgatttca ttgtcaacag ctaagaacac aactgtctta gcttcttttt
     3181 ctagttcttc tagtttatct tgataagtat tagaaatatc catgccatcc agcattttag
     3241 catttccaag taaaacttgt tttccattga ttcgccctga aacacctttc ccgtgcaagg
     3301 cctgaaaatt ttcaacagtt tgaaactcaa gtccagcttc actcgctcgc ttaacgatag
     3361 cctcagccag tgggtgttga gaagcatctt ccaaggaggc tgccaatcca aacacttcta
     3421 cttcgtcgcc gatgacatct gttaccacag gtttcccttc cgtcaaagtc ccggtcttat
     3481 caaagacaag ggtttgaact ttctggattt cctgtaagac agttccattt ttgaggagaa
     3541 cccccatctt ggcactacgt cctgtcccca ccataagggc tgtcggtgtt gcaagtccca
     3601 aggcacaagg acaggcgata atcaaaaccg ccactccgta gagaagagag gacacaaagc
     3661 tagctccaag cacaaccaca ctatccctga gcaagacgaa ccaaacccaa aaggtcatga
     3721 ttcctaaaat gacaactact gggacaaaaa tccctgaaat cttatccgtc aagtcctgaa
     3781 tcggcgcacg acttgtctga gctttcttca caaaatccac aatctgagcc aaaacagtct
     3841 ctgagccaac tttttctgct ctaaagacaa gcgttccact atgattgatg gttgagccaa
     3901 tgacagtatc tccaactgtc ttgtccacag gcagactctc acctgtcacc atggattcgt
     3961 caatactaga gacaccttct actacgacac catcaacagc aatcttttca ccgggacgca
     4021 ctcgaatcag gtcgcctacc ttgacttgtt ccaaaggaac ttggacataa ctatcatcac
     4081 tcaagacttc tgcggtttta gcttgcaagt ccagtaattt ctccacagct tgggacgtat
     4141 tttttctcat tttttcctca aaaactgctc ccaaaagaac gaaaaagagg ataaatccag
     4201 cactttcgaa gtaaacaggg agaccagcaa agagagcaac taggctatag aaataagcca
     4261 ctagagttcc cagcgcaacc aaggtatcca tgttggcatt gtgcttttta aaactggccc
     4321 aagcactctg gatatatggc ttacctgcaa ctaacataat aggcgttgtt gctagaaagg
     4381 ttccccaatg catgacttga tgactaatgc tacctgtcaa catcccaatc atgagaatca
     4441 caagaggcac agtaaagata ctagtaatcc aaaaacgttg caggagagat agagattttc
     4501 gagtcttctc aacgactgta tagcttccct tttgcatctt catgccacaa aaaaattcat
     4561 gtcgccctaa ttcttgaggt gtaaaacgaa tgactttctc ctcatctacg ccgattggtt
     4621 ccaagatacc ttcttcttca aacagaattt ccttataaca gtttgaagga gtagcacgat
     4681 gaaaggtaat ctcagctgga attccctttt gaagctggat atgggctgga tgatagcctt
     4741 tttcagatcg gatacggatt ttttgaatgc cattttctaa gcttgctttc acaatttctg
     4801 tcatagtctc cacctactct acaatcatct tgccgtgcat catgttcata ccacaagcaa
     4861 agccaaattc tccagcctgt tcaggcgtga tttccactac atactcttcc cccattggca
     4921 ggttcgtatg tacaccaaaa tctggaaaaa caatttgatc cagacatggt gaaggatcct
     4981 tgcggtcaaa gacaatgcgg gctggcactg atttcttgag gacaatcaac tcaggagtat
     5041 agcctcccat gacttccact cgaatctctt ggtatccgtt tttttgctgg gctttttgtc
     5101 cagatttttc aggatttttg aaaaaccaaa acaagataaa cgcgataagg gcaatacaaa
     5161 taatggttac aatactattt aacatgacgt ctcctttaca tacaattaca tcttacttct
     5221 gttacagcac ttgatttctt ctctgaaatc acagcttcca agtcttccaa gtcagtctga
     5281 gtaaattcac attctacaat caagtcagcc aacaaattcc taatcctacg ggaacaaacc
     5341 ttgtctttga tatcttggac aagtaaatcc cgactttggt ctagagttaa aagcgctgaa
     5401 taaacaaagg acttgccttc ttttttccga gtcaaacact ctttctcaac tagacgagcc
     5461 aaaagtgtct gaaccgtgga cttggaccag tcaaaccgct ctgccaaaac cctaatcaaa
     5521 tctgtactgg tctgctcccc ctgcatccaa ataatcttca tgacctgcca ttctgcatct
     5581 gaaatctgca ttaccatacc tccaaaatct acatttgtca attacactca acagtatact
     5641 cccaaaatct acatttgtca attatagaaa taatattttc ttcgaaaaat agaattttaa
     5701 tcatttgaaa aacgatttgc agtcaaatat tactatataa acaataaaaa tatgctatac
     5761 taaagaaaaa agaaaacaac cactaggggt gcgtaaagct gagattaacg actgttagat
     5821 ccctctgact caatctaggt aatgctagct gatggaagtg gaaatgataa tggggactag
     5881 cagtcttcta ttgcctttct aaaacagact agcttgttct taagaataca aacttcagtt
     5941 ggttgggagg ttttagatga cttatttacc cgttgctttg accattgcag ggactgaccc
     6001 tagtggtggt gctggcatta tggcagattt aaagtcattc caagcgagag atgtctatgg
     6061 agtggctgtt gtaaccagtc ttgtcgctca aaataccaga ggtgttcagc taatcgagca
     6121 cgtttctcct caaatgttga aagcccaatt ggagagtgtc ttttctgata ttccacctca
     6181 ggctgtaaaa actggaatgt tggctactac tgaaatcatg gaaatcatcc aaccctatct
     6241 taaaaaactg gattgtccct atgtccttga tcctgttatg gttgctacaa gtggagatgc
     6301 cttgattgac tcaagtgcta gagactatct caaaacaaac ttactacctc tagcaactat
     6361 tattacgcca aatcttcctg aagcagaaga gattgttggt ttttcaatcc atgaccccga
     6421 agacatgcag cgtgctggtc gcctgatttt aaaagaattt ggtcctcagt ctgtggttat
     6481 caaaggcgga catctcaaag gtggtgctaa agatttcctc tttaccaaga atgaacaatt
     6541 tgtctgggaa agcccacgaa ttcaaacctg tcacacccat ggtactggat gtacctttgc
     6601 tgcagtgatt actgctgaac tagccaaggg caagagtctt taccaggcag ttgataaggc
     6661 caaggccttt atcacaaaag ctattcaaga tgcccctcaa ctcggtcatg gttctggtcc
     6721 agtcaaccat acaactttta aagattaaga aaaaaaactc tctagttccc actttaaggg
     6781 aattagagag tttttatact cttcgaaaat ctcttcaaac tacgtcagct tccatctgca
     6841 acttcaaaac actgttttga gctgacttcg tcagtcttat ctaaaacctc aaggcagtac
     6901 tttgagcaac ctgcgactag ctttctagtt tactctttga ttttcattga gtattaatta
     6961 ggaaagaatg tcatgcaact ttgttaaaaa ggcttgcgtt tttgcctcaa tatcttctgc
     7021 ttgcatcaaa tcacgtacaa cagctacacc agctatgcca gtgcccataa gctgatcaat
     7081 attctccgaa gtcaagccac caatagcaac tattggaatg gcaaccgttt ggcaaattgt
     7141 tttcaaggtt gaaatcaggg tgatgggtgc attttccttg gtggttgtcg gaaaaatggc
     7201 tcctgtcccc aagtaatctg cacctgattt ctccgcttcc agagctcttt taaccgtttt
     7261 agcggtgaca ccgaggattt tttcaggacc caagacttgt cgggcaaccg aaactgggag
     7321 ttcgtcatct ccaatatgca gaccagcagc atcaaccgca agacaaacat ccaaccgatc
     7381 atcgattatc aagggtacct gataagcatc tgttatttcc ttgacttgtt ttgccagttg
     7441 ataatattga ttggttgtga gatttttttc tcgcaattgg actatggtaa cccctgaacg
     7501 gcaggccgtc tcaacttttg caagaaagct ttccacggaa tcttgatagc gattggttac
     7561 cagatatagt ctaagtgctt ctctattcat aaacctctcc tttgatggta tctagccaat
     7621 tttcatctct tcttaggagc gaaagctgat tgagtacttg gtaacgaaat tcttccaatc
     7681 ccattccttg aacaactatt ttctcagcag cgatattgag ataagagact gctaagcaag
     7741 aagcttcaaa accagtcttt ccttggctga gaaaaacggc tgttaaggct ccaaccaaat
     7801 ctcctgtccc tgttatccag tctaattctg cacagccatt tcccagtaca gcgacctgat
     7861 ttttcgaaac gacgaggtcc ttgggacctg tgactaagaa agacatacca tgataggtct
     7921 gacaccagtc tttcaagact tgaagcaaat cctccgtttc ttgatcttta gcactcgcat
     7981 cgaccccaac gccgtggtgc tttaatccaa caagacttcg aatttctgac atgtttcctt
     8041 taaggaccgt aggtctatag tctaaaaggt ctctgactaa gctcttacga atggatgaag
     8101 ctgttacgcc aaccgcatct actaccattg ggagacaaac ttgagctgca taagaagctg
     8161 ctatacggat tgctttctcc ttctcagctg acaaatgccc caaattgatg aagagagcct
     8221 gactttgctt agtaaaatca agaacctcac ggggatcatc tgccatgaca ggtttgcatc
     8281 ccagagccaa aatcccattt gccagcatct cacaagaaat ctcattggtc atacagtgaa
     8341 tgagggaact agagcctata ggaaagggat ttgtaaattc ctgcatcagt ctatcctttc
     8401 actaaagaaa tatccctgca cttttttaaa gaattcctgc ttgattaaaa atcgaaaggc
     8461 aataaaggaa atcgctgtac caatcaaggt tgctccgaaa aatcgaggcg tgtagataaa
     8521 ccagctaagc ttagcagctg atcctgtaaa gagtaccata acaggatagg aaacaataga
     8581 accaataata cctgttccca caatttctcc caaggcagaa aagtaaaatt ttcgaccgta
     8641 cttataaaag agacctgcta gaagggctcc aaaagtcgct cctgtgagag ctaaaggcgg
     8701 aatcccttga gtcgtcatac ggataaaggc tgtcactgta gccatggcca aggcataaac
     8761 aggtcccatc atgattcccg ctagaatatt gactacactg gacatcggtg ccattccctc
     8821 aatccgaaag ataggtgtaa ggactacatc aagggcagtc atcatagata aaatggtcaa
     8881 tttgtgaact tgtagttggt gctttctcat gtttctattc ttctcctttt tctaaagact
     8941 gtaaatcgct cttccatgtc tggtgttggt aagccatctc ccaaaacttg gcttccatat
     9001 gaacactgat gtggaaggca tctagcattt tttgcttatc tgtctcatca ctttctcgat
     9061 agagctgatt gaccagtgct ccctcctctc tgatctgttg ctctaactca tccgtaatat
     9121 aagtttcaat ccattgttga tagagaggat ttggtgatgg tttaagatta agtgatttgc
     9181 ctatatcatg gtataaccaa ggacaaggaa gcaagcttgc aaaagctatg gctaagttcg
     9241 gttctgcaaa ttgcctataa atatgagaaa tgtaatgata acaggttgga gcgattggat
     9301 gttgctccat ttcctggtcg ttgatttcca attccttgaa aaattgttgg cgaataaata
     9361 actcaccctc cactaaaccc tgagcatttt gtttcaagag tcttttcatc tcttggtttg
     9421 aagtcttatc agccaaaaga tgatagattt ctgagaaagc cttcagatag taggcatcct
     9481 gaatcaggta atagcggaaa atggcaggtt ctaaattccc ctcttgtaat tgtaaaataa
     9541 agggatgatg aaaggaagcc tgccaagctt tcttggataa ttccatcgca atatctgtaa
     9601 attccataat aactccttta taaaaataga ctggtttgaa gcaataaaaa gaaaagcagg
     9661 tagattaatt ttgttttttt aggaatataa aaagtccgat agctattctt caactgtgca
     9721 tgttcgtcat atccgtgagc agatagagct ctcaggtaaa gatggcgcca cctaaagact
     9781 gtcatcagaa ccttactgta aatcaagggc gaccaaaaat gtagtttttg accacgtaat
     9841 aggcaagctt ctttgaggga cttgatttct tgctgaatga gaggaaaaga attgaatacc
     9901 acaatcaagg cataggacca agagcgtgat agcccctttt gagccaagta caagagaagc
     9961 tcttttagtg aaatagagga aacaaagaca aggccgatac aaactgtcac aaaggccctc
    10021 gttccaagca tgactgcctg tgaagcatct ccgtgtaact gaactgccca gtagttggca
    10081 aaagatggta aaatggcaag tatgatcatc caagctaaca ttttaaatcg acggtaatag
    10141 agcataaaga gaatacaaaa tgcgactacc gaaagattca gagcaatcga aggaatgaaa
    10201 gatgtttcca aggataaaat cagcaagaag agactgataa tcggtgtctg ggttgctact
    10261 ttgaccatac tatctcacct ccccttgggt attgctactc tgagatgtaa gtggtttggt
    10321 aatggtcact tctttcacat gccgaagacc ctgactagtc atctcaatcc aataatcaac
    10381 cacagaaatc aaagggtcta aacgatgact aatgagcaga aaacttcttc cttgattcct
    10441 ctcctccaca atccacttgc aaaaataatg gcaggctcta tcatccaaac ctgcaaaagg
    10501 ttcatctagc aagatcacgg aagccttact ggtcaagatg gtcaggagct gaagaatttt
    10561 ttgctgacca ccacttaatt gatagggact cttatcgact gcctgctcca aatcaaaata
    10621 tcgtaaagct tgaaaaatcc gctgatttct ttcagaatca ggtccatcta attgaagctc
    10681 ctctcgcaga ctgactcgga taaactgctt ctcagcttcc tgaacaacac cagtcagatc
    10741 acgatacaaa ctctttttct ttttcaggac cgaacccttc catgcaatgc gccccttata
    10801 cttttgaaat tgaagaatag accgaaagag ggttgatttc ccgacaccat tgtcacccag
    10861 gatacaggaa atcccttgat agaatgtgaa atcagcaatt gaaaagaggg ggcgatttcc
    10921 aaactcacaa gtcgttctat ccatatggaa tagttccggg ctagaagcaa cttcctttga
    10981 agcaacctgt gtcatctcat aggaagggat ttgaaacact tcccttagtt ttccgtctct
    11041 tagctcaacc atatggtcga tataggcttt atagtcagat aaatcatggt cgcacaaaat
    11101 aactgtcttt ccatcaagag ccaactcttt tagaatctcc aatatctcga ttctgctctt
    11161 gcggtcaatg gaagcaaaag gttcatccaa gagatagacc ctaggattca tggcaaagag
    11221 gacagccagc gctgcttttt gcttttcccc acctgatagg tgatgaatgg gacggtgtaa
    11281 gattgcctcg caacgacatt gctggacaac ctctgctatt ttagaatcaa tttcctgaag
    11341 gtgatagccg atattctcca aggtgaaaat cagctcctca aacaagctct ccatggtaaa
    11401 ttgatgattg ggattttgaa agagaatgcc aaccttctgg acacgtgcga tgatagaaag
    11461 ttgactgacc tcgctcccat ctatcaggac ttgaccacta tagggaagag aactgacttg
    11521 agcaatgact tgaaaaaggc tggattttcc tgaaccactg ctcccaacta acaaggtaaa
    11581 ggcttgcgca tgaaaagtaa aatcaaacgg ctcagagaag attggggact gaatcgctcg
    11641 tagttccaga cccatctatg cttttcctcc agttgcaaac tgatgataga gtttgacaat
    11701 ggcacgaacc aagatggtac agaagaaata aacagaaata aaacgtacca caagtaagga
    11761 aaggacaaac ggaagggaaa aggcgtagta acctaactta atgtattcat agacaaagct
    11821 aacaagcgta atcccaatac tattagcagt tagagagagc caactttcat agcgattctt
    11881 agttacgata aaaccaaatt cacttcccaa accttgaacc aagccagaca aaagagctcc
    11941 tagaccaaat tggctaccat aaaggacttc agcaagcgca gctagcactt ctccaatcgt
    12001 tgcacttccg actctcggaa caaagatggc agcaatgggc gcagccatac accagagacc
    12061 gaagaggatt tcattggcaa aggcctgcaa accaagaggt gttaagagta gactgagaat
    12121 attatacaca tatcctgaac caacgaaaac cccaccaaaa aagatagaca agaaagcaag
    12181 caagataaca tcttttaact gccatttttt caacataaaa aactcctttt tttaaagaaa
    12241 agtggggcac tcaagaagac cgacctaaat actttgtata gcagactgaa tttagaacag
    12301 tacacaagaa tgctaaaata tttctagaaa ttaatttgaa ttttctaatt gatttgttcg
    12361 catcttattt caatctacta tagaaaaaag tctttaaaat tataaaacgc atcatatcaa
    12421 gatttttcaa aaa
//
LOCUS       DBERDK010000053        12333 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.053, whole genome shotgun sequence.
ACCESSION   DBERDK010000053 DBERDK010000000
VERSION     DBERDK010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 12333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12333
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.053"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            127..3228
                     /locus_tag="ACL73L_001514"
     CDS             127..3228
                     /locus_tag="ACL73L_001514"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HGS0772472.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRAAKEYGYTHLAMMDIDNLY
                     GAFDFLEITKKYGIHPLLGLEMTVFVDDQEVNLRFLALSSVGYQQLMKLSTAKMQGVK
                     TWSVLSQYLEDIAVIVPYFDRVESLELGCDYYIGVYPETLASEFHHPILPLYRVNAFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASSLEKLFQECFPQALDNLEKLISG
                     ISYDLDTSLKLPRFNPARPAVEELRERAELGLVQKGLTSKEYQDRLDQELSVIHDMGF
                     DDYFLVVWDLLRFGRSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPE
                     YELSAITKKISFRDNLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLA
                     ETEGIHLKIEEIDLEDKETLALFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDPVLEDILAPTYGIMLYQEQVMQVAQRFAGF
                     SLGKADILRRAMGKKDASAMHEMRASFIQGSLEAGHTVEKAEQVFDVMEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQIMLNSSNSDYLIDALEAGFEVASLSINTIPYH
                     DKIANKAIYLGLKSIKGVSNDLALWIIENRPYSNIEDFIAKLPENYLKLPLLEPLVKV
                     GLFDSFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQESEDWTEQEKFYMEQELLG
                     IGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVQKIKVIRTKKGENMAFLQADD
                     SKKKLDVTLFSDLYRQVGQEIKEGAFYYVKGKIQLRDGRLQMIAQEIREAVAERFWIQ
                     VKNHDSDQEMSRILDQYKGPIPVIIRYEEEQKTIVSPHHFVAKSDELEAKLNGIVMKT
                     IYR"
     gene            3310..4317
                     /gene="pfkA"
                     /locus_tag="ACL73L_001515"
     CDS             3310..4317
                     /gene="pfkA"
                     /locus_tag="ACL73L_001515"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HGS0772473.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSAAEFGQKLKEAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPHKADIELSSLNKSL
                     S"
     gene            4374..5879
                     /gene="pyk"
                     /locus_tag="ACL73L_001516"
     CDS             4374..5879
                     /gene="pyk"
                     /locus_tag="ACL73L_001516"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HGS0772474.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
                     VAKDDATREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATNSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAIEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
     gene            complement(6098..6451)
                     /locus_tag="ACL73L_001517"
     CDS             complement(6098..6451)
                     /locus_tag="ACL73L_001517"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HGS0772475.1"
                     /translation="MTLQKANTKLEKPINQPLSSHIFRHTLLSTLAEKNAIMVRVEHK
                     NAKTVNNIYTHVSKRMEQATLEVLNTISLNRKYIRSNLDKYITIAKTFVEIHLNFLIN
                     LCISYFILNENQRAN"
     gene            complement(6621..6836)
                     /locus_tag="ACL73L_001518"
     CDS             complement(6621..6836)
                     /locus_tag="ACL73L_001518"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HGS0772476.1"
                     /translation="MEPKIRNSDYLFIKNTPQIDYNTIGIFQVDGTNYVKKLRQGYLK
                     KTQSRILRHPPRRKQPYSYHRESCQAI"
     gene            7684..8556
                     /locus_tag="ACL73L_001519"
     CDS             7684..8556
                     /locus_tag="ACL73L_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HGS0772477.1"
                     /translation="MKKSRKLATLGICSALFLGLAACQQQHATSEGTNQRQSSSAKVP
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDAFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFDSQDKEEFDILFSRVPTEATTDVKVHAEKMETFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPADDGFLFVEKLTFEEPYQAIKFASKEDCYKYLDTKYADYTGEGL
                     AKPFIMDNDKWVKL"
     gene            complement(8705..9045)
                     /locus_tag="ACL73L_001520"
                     /pseudo
     CDS             complement(8705..9045)
                     /locus_tag="ACL73L_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            9188..9499
                     /locus_tag="ACL73L_001521"
     CDS             9188..9499
                     /locus_tag="ACL73L_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772478.1"
                     /translation="MELKDFTEKEQEMIKKRLTMSNISDKETTEKILALVPQDLIKRI
                     PFFVRKHATTRTIKRISIEYPELYAVAQTSGEIPEKEREELRQIITTIFEQKMNKHSI
                     K"
     gene            9503..10219
                     /locus_tag="ACL73L_001522"
     CDS             9503..10219
                     /locus_tag="ACL73L_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGS0772479.1"
                     /translation="MKKYFISGLGSNAYHSKDFFQELDSQVYFLNPYEKHLRDETELK
                     SWFKNEIVEEESICLIGHSLGGDLARYFASEFEEVKKLILLDGGYLDLDKILPLDTEL
                     EETKNYIKSQIVSDLALLISKEKSEAKHWSENMEKAVRQSYHWNVEYNRYELAINYEN
                     IEAILRLRRKIQAFKREVGDTLFISPRYPNGATWREEALKELPDYFNTIFLENFGHEL
                     YTQAPKEIASLMNEWLAYFL"
     gene            complement(10349..11164)
                     /locus_tag="ACL73L_001523"
     CDS             complement(10349..11164)
                     /locus_tag="ACL73L_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HGS0772480.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKQVKTHSHLNSRLAKMTSISLLAIPLVIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTDLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSIQITNEN
                     YMEVMEAIYDYPDEFEGKTIQFTGFVYNDPSHANSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWITAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
     gene            complement(11161..12066)
                     /locus_tag="ACL73L_001524"
     CDS             complement(11161..12066)
                     /locus_tag="ACL73L_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HGS0772481.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIVSGLIEVYIT
                     PDKVYHFLPRNRWGRIFFGTFVGILFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSILVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSYLSSAKKVFQVFVQAIDEFFDTGRYLVFGCLFASIIQVYVPTRILTSISATPLFA
                     ILLLMLLAFLLSLCSEADAFIGASLLSSFGLAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLAYSLLIGVIL"
     gene            complement(12067..12198)
                     /locus_tag="ACL73L_001525"
     CDS             complement(12067..12198)
                     /locus_tag="ACL73L_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HGS0772482.1"
                     /translation="MAVKFTKRDDLDKMFEEFAKLPDLKQVTFPDDKEKKVKAEKKN"
BASE COUNT         3855 a         2149 c         2621 g         3708 t
ORIGIN      
        1 aaaacgatga caaaaataca aatgaataga aaataaatta gtaagctgat gaaatttttc
       61 tcaagagaag ctatttatag gtgaaaatgg tataatatag tgagaaggat agaggagaag
      121 tgtaaattga tcgcacaact agatacaaaa acagtctata gttttatgga aagcgtcatt
      181 tcgatcgaaa agtatgtgag agcagctaaa gaatacggct acactcattt ggctatgatg
      241 gatattgaca atctttatgg cgctttcgac tttctagaga ttacaaaaaa atacggcatt
      301 catcctttgc tagggcttga aatgacagtg tttgtagatg atcaggaagt gaatttgcgc
      361 tttttagctc tatctagtgt gggctatcag cagttgatga agctttcgac agccaagatg
      421 cagggggtga aaacttggtc agtcctgtcc cagtacctgg aggatatcgc ggtcattgtg
      481 ccttattttg atagagttga gtcgttagaa ctaggctgtg attactatat aggggtttat
      541 ccagaaacac tagcaagcga atttcatcat cctatcttac ctctttatcg ggtcaacgct
      601 tttgaaagca gggatagaga agttcttcaa gttttaacag cgattaaaga aaatctaccg
      661 ctcagagaag ttcccttgcg ttcgagacaa gatgtcttta tatcagcaag ttctttagag
      721 aaactattcc aagagtgttt tccgcaagct ttggacaatt tagaaaagct tatttcaggc
      781 atttcttacg acttggatac tagtctgaaa ctgcctcgtt ttaatccagc tagaccagca
      841 gtagaggagt tgagagagcg tgctgaactg gggcttgttc agaaggggtt gactagtaaa
      901 gaatatcaag atagactaga ccaagaattg tctgttattc atgatatggg ctttgatgat
      961 tatttcttgg ttgtttggga tttgttgcgt tttggacgat cgaatggcta ttatatggga
     1021 atgggaaggg gttctgcagt aggcagtttg gtttcttatg ccttagacat cacggggatt
     1081 gacccagtag agaaaaatct gatttttgaa cgctttctta atcgtgaacg ctataccatg
     1141 cctgatattg atattgatat cccagatatt tatcgtccag attttatcag atatgttggt
     1201 aataaatatg gtagtaaaca tgcggcacaa atcgttactt tttcaacctt tggagccaag
     1261 caagctcttc gagatgtttt gaaacgcttt ggtgtgccag agtatgaatt atctgcaatt
     1321 actaagaaaa tcagttttcg tgacaatctt aagtcggcct atgagggcaa tctccagttt
     1381 cgtcagcaaa tcaatagtaa gttagaatac caaaaagctt ttgagattgc ttgcaagata
     1441 gagggctatc caaggcaaac ctctgtccat gcggctggtg ttgtaattag tgaccaagat
     1501 ttaaccaact acattcctct aaagtatggt gatgaaattc cactgactca gtatgatgct
     1561 catggagttg aggctagcgg acttttgaag atggactttc tgggactacg aaatttgacc
     1621 tttgtccaga agatgcaaga gttgcttgct gaaacagaag gtattcatct gaaaattgaa
     1681 gaaatcgatt tagaagacaa agaaacgtta gctttatttg cctctggtaa tacaaaaggt
     1741 atctttcaat ttgagcaacc aggtgccatt cgtctgctta agcgtgtgca accagtctgt
     1801 tttgaagatg tcgtcgcgac tacttctcta aatcgaccgg gtgctagtga ctatatcaat
     1861 aattttgtgg caagaaagca tgggcaggaa gaagtgactg ttctggatcc agtactggag
     1921 gatattttgg ctccaaccta cggcataatg ctctatcagg agcaggttat gcaggttgcc
     1981 cagcgatttg ccggatttag tcttgggaaa gccgatattt tgcgtcgggc tatggggaaa
     2041 aaggatgcct ctgccatgca tgagatgagg gcttccttta ttcaaggttc attagaagct
     2101 ggtcatactg tggaaaaagc agagcaggtc tttgatgtta tggagaagtt tgcaggctat
     2161 ggttttaaca ggtcacacgc ctatgcctac tcagcattgg ccttccagtt ggcttatttt
     2221 aaaacacatt atccagccat attttatcag attatgttaa attcttccaa cagtgattac
     2281 ttaatagatg cacttgaagc aggttttgaa gtagcctctc tatccatcaa caccattccc
     2341 tatcacgata aaattgccaa caaggccatc tatctaggtt tgaaatccat taaaggagtc
     2401 agtaatgatt tagctctctg gattattgaa aatagacctt attctaacat tgaagatttt
     2461 atagctaaat tacctgagaa ttatctgaaa cttcctctgc tagaaccttt ggtaaaagtt
     2521 ggtcttttcg attcatttga aaaaaatcgt caaaaagtat ttaataactt agctaatcta
     2581 tttgaatttg tgaaagagtt gggaagtttg tttggagatg ctatttatag ttggcaggaa
     2641 tcggaagatt ggacggaaca agaaaaattt tatatggaac aagagctttt agggataggt
     2701 gtcagcaaac atccactaca agctattgca agtaaggcta tttacccgat taccccaatc
     2761 ggaaatttgt cagaaaatag ctatgctatt atcttggttg aagttcagaa aataaaagtg
     2821 attcgtacca aaaagggtga aaatatggcc ttcttacagg cagatgatag taagaaaaaa
     2881 ttggatgtca ctctcttttc agacttatat cgtcaggttg gacaggaaat aaaagaggga
     2941 gccttctact atgtaaaagg aaaaatacaa ttacgtgatg gccgtctgca aatgattgca
     3001 caagaaataa gagaagcagt tgctgaacgc ttttggattc aggttaaaaa tcatgactcg
     3061 gatcaagaga tgtcacgtat tttagaccag tataaaggcc caatcccagt catcatccgg
     3121 tatgaagagg aacagaaaac catcgtttct ccccatcatt ttgtagctaa atctgatgaa
     3181 ttagaggcga aattgaatgg aatagttatg aaaacgattt atcgctaaaa ttacggaaaa
     3241 tagaagaatt ttcaacgtaa atgtggtata atcagtaaga atgttaaaag aaaaaggagc
     3301 ataaccaata tgaaacgtat tgctgttttg actagtggtg gagacgcccc tggtatgaac
     3361 gctgccatcc gtgcagttgt tcgtcaagca atttcagaag gaatggaagt gtttggtatc
     3421 tatgacggat atgctggtat ggttgccggt gaaattcatc ccctagatgc agcttcagta
     3481 ggggacatca tttctcgtgg tggtactttc cttcactcag ctcgttaccc agagttcgct
     3541 caacttgaag ggcaacttaa agggattgag caattgaaaa aacacggaat tgaaggtgta
     3601 gttgttatcg gtggtgacgg atcttaccac ggcgctatgc gtttgactga acatggcttc
     3661 ccagctattg gtcttccagg tacaatcgat aacgatatcg ttggtactga ctttacaatc
     3721 ggttttgaca cagcggttac tactgccatg gacgctatcg ataagattcg tgatacatca
     3781 tcaagtcacc gtcgtacttt tgtaatcgaa gttatgggac gtaacgctgg tgatatcgct
     3841 ctttgggctg gtattgcaac tggtgctgat gaaatcatca tccctgaagc aggcttcaag
     3901 atggaagata tcgtagcaag catcaaagct ggttatgaat gtggtaaaaa acacaatatc
     3961 atcgtcttag ctgaaggtgt gatgtcagcg gctgaatttg gtcaaaaact taaagaagct
     4021 ggagatacaa gcgaccttcg tgtaacagaa cttggacata ttcaacgtgg tggttctcca
     4081 actgcgcgtg accgtgtttt ggcgtcacgt atgggtgcac atgctgttaa acttcttaaa
     4141 gaaggtatcg gtggtgttgc ggttggtatt cgtaacgaaa aaatggttga aaatccaatt
     4201 cttggtactg cagaagaagg ggcattgttt agccttactg cagaaggtaa gattgtggtt
     4261 aacaacccgc acaaagccga tattgagcta tctagcttga ataagagctt gtcataattt
     4321 acaatttagt taataagacc aaaaaggtca tatataaagg agtcacaaaa atcatgaaca
     4381 aacgtgtaaa gatcgttgca actttgggtc ctgcggtaga aatccgtggt ggtaaaaaat
     4441 tcggtgagga cggatactgg ggtgaaaaac ttgatgttga agcttcagct aaaaacattg
     4501 ctaaattgat tgaagctggt gctaacacat tccgattcaa cttctcacac ggcgaccacc
     4561 aagaacaagg tgagcgtatg gcaactgtta aacttgcgga aaaaattgca ggtaaaaaag
     4621 ttggtttcct tcttgataca aaaggacctg aaatccgtac agaattgttc gaaggtgaag
     4681 ctaaagaata ttcatacaaa actggtgaaa aaattcgtgt tgcaactaaa caaggaatca
     4741 aatcaactcg tgaagtgatt gcgttgaacg ttgctggtgc tcttgatatc tatgatgatg
     4801 ttgaagttgg tcgtcaagtt ttggttgacg atggtaaact tggtcttcgt gtggttgcta
     4861 aagatgatgc aactcgtgaa tttgaagttg aagttgaaaa cgatggtatc atcgctaaac
     4921 aaaaaggtgt gaacatccct aacactaaaa ttcctttccc agctcttgct gaacgcgata
     4981 acgacgatat ccgtttcggt cttgaacaag gtatcaactt catcgcaatt tcattcgtac
     5041 gtactgcaaa agatgtgaac gaagttcgtg caatctgtga agaaactgga aacggacatg
     5101 ttcaattgtt cgctaaaatc gaaaaccaac aaggtatcga taacttagac gaaatcatcg
     5161 aagcagctga tggtattatg attgctcgtg gtgatatggg tatcgaagta ccgttcgaaa
     5221 tggttccagt ttatcaaaaa atgattatca agaaagtcaa tgctgcaggt aaagttgtta
     5281 tcactgcaac aaacatgctt gaaacaatga ctgaaaaacc acgtgcaact cgttcagaag
     5341 tatcagacgt attcaacgct gttatcgacg gaactgacgc tacaatgctg tcaggcgagt
     5401 ctgcaaacgg taaataccca ctcgagtcag taactacaat ggctacaatc gacaagaacg
     5461 ctcaagctct tcttaatgaa tacggacgtc ttgattcaga ttcatttgag cgtaactcta
     5521 agacagaagt aatggcttct gctgttaaag atgctactaa ctcaatggat atcaaattgg
     5581 ttgtaactct tactaagaca ggtcatactg cacgtttgat ttctaaatac cgtccaaatg
     5641 ctgacatctt agcattgaca tttgacgaat tgacagaacg tggcttgatg ttgaactggg
     5701 gtgttatccc aatgttgaca gatgctccat cttcaactga cgatatgttc gaaatcgctg
     5761 aacgtaaagc gatagaagca ggtctcgttg agtcaggcga tgatatcgtt atcgttgctg
     5821 gtgtgccagt aggagaagct gttcgcacaa acacaatgcg tatccgcaca gtacgttaag
     5881 aaaaatataa aaacctatca tatccagctt tagagcttgt gtgataggct ttttgtatag
     5941 agggtaagaa ataggcaaaa ctttcataat ggattgatac tcttcgaaaa tcaaattcaa
     6001 accacgtcag cgtcgcctta ccgtatatat gttactgact tcgtcagttc tatctacaac
     6061 ctcaaagcag tgctttgagc aacctgcggc tagcttccta gtttgctctt tgattttcat
     6121 tgagtatgaa ataagatatg cacaaattga ttaggaaatt caaatgaatt tctacaaatg
     6181 ttttagcaat cgtaatgtac ttgtctagat tcgatctgat atattttcga tttaatgata
     6241 tggtatttaa aacctccaaa gtagcttgct ccattctttt acttacgtga gtgtagatgt
     6301 tatttactgt tttagcgttt ttgtgttcca ctctaaccat tatagcattc ttctcagcta
     6361 gtgtactaag gagtgtgtgc ctgaaaatat gggaactaag gggctggttt atcggtttct
     6421 ctagtttagt atttgccttt tgcaaagtga tcttaaatgc ctttctctaa atttacatat
     6481 cactattgtt taacaaaatc taatctattt taggtcactt attctttttt tgaaatgtag
     6541 aatgaacttt ttcaaagttt ttcgaatctt ttaaaatctg tttgttttat atcgccattc
     6601 tccccctttt ttaattctcc ctatatagcc tgacaacttt cccgatggta cgaatatggt
     6661 tgctttcgtc taggtggatg tcggagtatt cgggattgag tttttttgag gtagccttgg
     6721 cggagtttct tgacatagtt agtgccgtct acttggaaga tgccgatggt attatagtca
     6781 atctgtgggg tattcttgat aaataggtag tcgctgtttc ttatctttgg ctccatggac
     6841 ttgctgacga cataagcgat tgggtcgtag tcgtctggga taatggaaac tccataacta
     6901 aatcgtcgtc ctgcatcaag cggctacctg cagagataaa ctacctaaca cgagagtaag
     6961 tagtctgtct gtagtcgtcc agtctgatga tttttacgat acttcgtttt tctgatcata
     7021 cagttgcctc tcggcatagg tcagaacttt accttgtctg ggtggtgccc gttggtcgta
     7081 gatagattgg atatcgctag gagaatcctt ttgaactgga ggaaagaggg catcaatcaa
     7141 gctactgaat actttaacta agtcaaatat agtatttttc ttagtagacc taaccctttt
     7201 ttcataattt ccaatggtgt ttttacttat accaatctta gtacccaatt cttattgagt
     7261 ccaaccatta ctagtctata ttgttttata gttgattgag tttggaatag tacgctgtag
     7321 ctgctaaaac atttctagga attaatttgc ctttcctaat agagttgttc atatcttatt
     7381 tcaatctact atatttttcg cctctaacaa tcgcaatctc ttctttatct acgaatgaaa
     7441 tcgctttcta tttttgtaag taaagcatca cacgaaatcc acgaaaatga aaacctttgt
     7501 tgtgttttcg taaaaaattt gttgacagag cacgaaacgc ctactataat taaatcaagc
     7561 ttaaggaaat aacaaaaaaa caaatcggag ggaaagacga tgaacatatt aaatgagaaa
     7621 gtaatcaata tctgtaaagc agtagttaaa gaaactttaa tccaagacat ttaggagaaa
     7681 gaaatgaaga aatcaagaaa attagccaca ttaggaatct gttcagcttt gtttttaggc
     7741 ttggctgctt gtcaacaaca acatgctact tctgagggga cgaatcaaag gcaaagcagt
     7801 tcagcgaaag ttccatggaa agcttcatac accaacctaa acaaccaggt aagtacagaa
     7861 gaggtcaaat ctctcttatc agctcacttg gatccaaata gtgttgatgc attttttaat
     7921 ctcgttaatg actataatac cattgtcggc tcaactggct tatcaggaga tttcacttcc
     7981 tttactcaca ccgaatacga tgttgagaaa atcagtcatc tctggaatca aaagaagggc
     8041 gattttgttg ggaccaactg ccgtatcaat agttattgtc ttttgaaaaa ttcagtcacc
     8101 attccaaagc ttgaaaagaa tgaccagttg cttttcctag ataatgatgc gattgataaa
     8161 ggaaaggtct ttgattcaca agataaggaa gagtttgata ttctattttc gagagttcca
     8221 actgaggcaa ctacagatgt caaggttcac gctgaaaaga tggaaacatt cttctcacaa
     8281 tttcaattca atgaaaaagc tcgaatgctg tctgtagtct tgcacgacaa tctggatggc
     8341 gagtatctgt ttgtaggcca cgttggggtc ttagtacctg ctgatgacgg tttcttattt
     8401 gtagagaaat tgactttcga agagccctac caagcgatta aatttgctag taaggaagat
     8461 tgctacaagt atttggacac caagtatgcg gattatacag gcgagggact ggctaagcct
     8521 tttatcatgg ataatgataa gtgggttaaa ctttaatagt gttatgatat gacagtaatc
     8581 ttaggatttt tatttctatt tttgatgagt tggtgggtta tttcaatcgt taatagccag
     8641 aaaccacgca aaaatgaaac atttattggc tatgtccaaa gatacgatat tgatgggaat
     8701 ggctatcaat cattaccgtg tcctcagaac tgagaggagt tcttgaaatc gtaaaaccac
     8761 tttgaacaag agttacttca acccattggc tccgacggat taagttgctt tcgtgaatac
     8821 caaaatcagc cgcaatttct tcataagtgc ggtattctcg cacatattga aagtggccat
     8881 aagaaggtct tctaggctta atttaggttt tcgtccacct tttgcgtgtt taagttgata
     8941 agctgttttt aatacagcta acatctcttc aaaagtcgtg cgctgaacac caacaagacg
     9001 cttaaatcgt gcatcagtta gttgtttact tgcttcataa ttcatagaac tattatacca
     9061 tgttttgttt cgcaggaaat ctaatgctaa aatcttatca gtgagaagat ttcttaaagt
     9121 ttattctttg tagtttcatc tgtattagta cataatttaa cttagagaaa ataggaggac
     9181 taatcgaatg gaattaaaag attttacaga aaaagaacag gaaatgatta agaaaaggct
     9241 tacaatgtct aatattagtg acaaagaaac tactgagaag attctggcgc tagtaccaca
     9301 agacttgatt aagcgaatcc cgttttttgt cagaaaacat gctacaacac gtacgattaa
     9361 acgcatttca attgaatacc ctgaactcta cgctgtagct caaacaagtg gtgagattcc
     9421 agaaaaagaa cgcgaagaat tgcgtcagat tatcacgact atctttgaac aaaagatgaa
     9481 taagcatagt attaagtaga gaatgaaaaa atattttatt agcggtttgg gaagcaatgc
     9541 ctatcatagc aaggattttt ttcaagaact agattcgcag gtctattttc taaatccata
     9601 tgaaaagcat cttcgagatg aaacagaatt gaaatcatgg tttaaaaatg agattgtaga
     9661 ggaagaatct atctgtctga taggccattc tcttggagga gatttagctc gttatttcgc
     9721 atcggaattt gaagaagtaa agaaactgat tcttttggat ggtggctatc tagatttaga
     9781 taagatttta cctttggata cagagttaga ggaaactaaa aattatatca aatctcaaat
     9841 tgtttcggac ttagctcttc ttatttctaa agagaagtcc gaagcaaagc attggtcaga
     9901 aaatatggag aaagctgtaa gacagtccta tcactggaat gttgagtata atagatatga
     9961 gttggctata aattatgaaa atatagaagc gatactccgc ctacggagaa aaatacaagc
    10021 ttttaagaga gaagtgggag ataccttgtt tattagtcct cgctatccta atggagctac
    10081 atggagagag gaagccctaa aagaattgcc agactatttt aatactattt ttttagaaaa
    10141 ctttggccat gagctttata ctcaagcacc taaagaaatc gctagtctaa tgaatgagtg
    10201 gctcgcttat tttctatgag ctacttgtat tgctggaaat ggtataagat gaaaatctac
    10261 aagtcactaa ttttcatata acaaaaaaac gaacaggctc tttttctgtt attcagaaga
    10321 gaacttgttc gtttttatct tgattttttt aaaaagctct atatacatag ggattttctg
    10381 gtttatcgac tttggtaaag ctgtcgattt ccaaggttgg aaggttttgt ttgagttctt
    10441 tatggtagtg attgaccagt tttcctttgg ctgtaatcca ggtgttgttt tcatactgcc
    10501 gggtattgcc cttggtcagc aatccataga caccagaatc tgcgatacag tggataatgc
    10561 cgaatcggaa cagaaattga ctattggcat gactggggtc gttatagaca aagcctgtaa
    10621 actggattgt cttgccctca aattcatctg gatagtcgta gatagcctcc atgacttcca
    10681 tatagttttc attagtgatc tgaatactat cttgggataa gtatttatcc gccgccgttc
    10741 gcatttcctt ttcataggct gattttgaaa aataagaact agtatctggt ttcaaatatt
    10801 ggcttgtcgt cccttcactt gtctgaatag ctagatccgt tccttccgat agggggaaat
    10861 gataaccttt agcagaaacg gtctgagaat ccaagctaac agttgggaaa gttaagccga
    10921 tgacaagtgg aatagccaga agagaaatac tcgtcatctt ggccaatcgg ctgttcagat
    10981 gactgtgggt tttgacttgc ttcatccaga tatacaattg aacaattgcc aagataaagg
    11041 aaagcaccat ggagatatag gccagatagg aatagtgcat gttgatgtac tggtttagtt
    11101 tgcccgacag atggaggtaa atagtcagtt caaaatagcc agctaaaact aaaaatcgaa
    11161 tcataggatg actccaatca agagagaata ggctaaaacg accagagtca caatcgtaat
    11221 gaagtgacta ataaatcgtg ctttcaagta atttttcatc atgagaatat ttttgatatc
    11281 cagcattggg ccaatgacga gaaaggccag aactggtgcc aaaccgaaac tcgagagtag
    11341 agaagcacct ataaaggcat ccgcctcact acagagcgaa agaagaaagg ctaaaagcat
    11401 caagagcagg atagcaaaaa gaggagtcgc actgatagag gtcagaatcc gagtcggaac
    11461 gtagacctgt attatagaag caaagaggca gccaaatacc aaataacgcc ccgtatcaaa
    11521 aaattcatca atggcctgca caaagacttg aaaaactttt tttgcagaac tcaagtaaga
    11581 aaaatcatgc tcatgacaag ccagacgatt ttctttctga atcggttctt gccagaaaaa
    11641 tcctagaaaa attcctagta ttacagccac aagaatggaa cccagagctc gtaatagtgc
    11701 gacatggaag gagttgccaa aggcagaata ggtcgcaaaa agaacaatgg gattgataac
    11761 aggtgctgtc acaagaaaag gaacggccgt gtaacttgga accttttttt ccagaaaacg
    11821 attgatgatg gggacgattc cacattcaca agaagggaaa agtataccga caaaggtccc
    11881 aaaaaagatt ctcccccaac gatttcgagg gagaaaatga taaaccttgt caggtgtgat
    11941 ataaacttca atcagccctg agacaatgct tcctatcaga acgaagggaa gggcttcaat
    12001 gataatggag agaaaaatgg ctccagtttg aagtacacta gatgggagtt gttgaaaagc
    12061 agtcatctag tttttctttt ctgctttgac ttttttctct ttgtcatcag ggaaagtaac
    12121 ttgtttcaaa tcagggagtt tagcaaactc ttcaaacatc ttgtccaagt cgtctcgttt
    12181 tgtaaatttt acagccatag gtgtacctcg attctaaatt ctacctctat tatactatta
    12241 ttttgcggaa aagggagttt tttaatttgt tttcgatagt tgatactctt cgaaaatctc
    12301 ttcaaaccac gtcagcttcg ccttaccgta gta
//
LOCUS       DBERDK010000054        11983 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.054, whole genome shotgun sequence.
ACCESSION   DBERDK010000054 DBERDK010000000
VERSION     DBERDK010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 11983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11983
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.054"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            172..825
                     /locus_tag="ACL73L_001526"
     CDS             172..825
                     /locus_tag="ACL73L_001526"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HGS0772483.1"
                     /translation="MEHSSWHALIKAQLPEGYFGKINQFMEQVYSQGTIYPPKEKVFQ
                     ALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANAFLKETGQEPIDWLR"
     gene            835..1299
                     /locus_tag="ACL73L_001527"
     CDS             835..1299
                     /locus_tag="ACL73L_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HGS0772484.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGE
                     TPQECAVREILEETGLKAKPVLKGVITFPEFTPDLDWYTYVFKVTEFEGDLIDCNEGT
                     LEWVPYDEVLSKPTWEGDHTFVEWLLGDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            1312..2580
                     /locus_tag="ACL73L_001528"
     CDS             1312..2580
                     /locus_tag="ACL73L_001528"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HGS0772485.1"
                     /translation="MLLIKNGRVMDPKSGLDQVCDVLVQDGKIIKIAPEITEEGAETI
                     DATGLVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQAVLQSAAKEKINVKTVATITKNFNGKNLTDFKALLEAGAVGFSDDGIPLESSKIV
                     KEAMEEAKKLNTFISLHEEDPGLNGVLGFNENIAREHFHICGATGVAEYAMMARDVMI
                     AYATKAHVHIQHLSKEESVKVVEFAQGLGAEVTAEVAPQHFSKTEALLLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKAPSGMTGLETSLSLG
                     LTYLVEAGELSLMELLEKMTYNPAKLYNFEAGYLAENGPADITIFDAKADRLVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            2594..2854
                     /locus_tag="ACL73L_001529"
     CDS             2594..2854
                     /locus_tag="ACL73L_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772486.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPAANRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRTCACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            2865..4226
                     /gene="pdrM"
                     /locus_tag="ACL73L_001530"
     CDS             2865..4226
                     /gene="pdrM"
                     /locus_tag="ACL73L_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HGS0772487.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPTILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKLKALHLEKRIPLNMDNIKEGVRLGLPIGGTVFAEVAIFSVVGLIMAKFSSLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASLYGNDPKFIDLTVRFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYL
                     GLLGYWGVAIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAK
                     SKR"
     gene            4380..5348
                     /locus_tag="ACL73L_001531"
     CDS             4380..5348
                     /locus_tag="ACL73L_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HGS0772488.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGPVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIYAD"
     gene            5364..6356
                     /locus_tag="ACL73L_001532"
     CDS             5364..6356
                     /locus_tag="ACL73L_001532"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HGS0772489.1"
                     /translation="METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSV
                     GMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAK
                     TRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
                     KSSIRDNNSVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRR
                     VVQAAEELAEEGISVEIVDPRTLVPLDKDIIINSVKKTGKVVLVNDAHKTSGYIGEIS
                     AIISESEAFDYLDAPIRRCAGEDVPMPYAQNLENAMIPTVESIKDAIRKTYNKE"
     gene            6574..7617
                     /locus_tag="ACL73L_001533"
     CDS             6574..7617
                     /locus_tag="ACL73L_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HGS0772490.1"
                     /translation="MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     DTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQI
                     EKVEEVPDNVTPYGEIERIPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRK
                     RVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPCINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQ
                     SFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            7663..9366
                     /gene="lpdA"
                     /locus_tag="ACL73L_001534"
     CDS             7663..9366
                     /gene="lpdA"
                     /locus_tag="ACL73L_001534"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003001382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HGS0772491.1"
                     /translation="MALEVIMPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKV
                     SMELEAEEDGYLIAILKGDGETVPVTEVIGYLGEERENIPTAGAASPEASPVPVASTS
                     NDDGKSDDAFDIVVIGGGPAGYVAAIKAAQLGGKVALVEKSELGGTCLNRGCIPTKTY
                     LHNAEIIENISHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVTV
                     HKGIGTITKDKNVLVNGSELLETKKIILAGGSKVSKINVPGMESPLVMTSDDILEMNE
                     VPESLVIIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDAEVSKNLRLILERKGM
                     TILTGTKLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCI
                     KVNEYMETSVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIY
                     TLPEVAAVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVIADKKYGEILGV
                     HIIGPAAAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKK
                     K"
     gene            9430..10419
                     /locus_tag="ACL73L_001535"
     CDS             9430..10419
                     /locus_tag="ACL73L_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HGS0772492.1"
                     /translation="MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVG
                     RHQNTIEEINRDYVRENGIEVVRRISGGGAVYHDLNNLNYTIISKEDENKAFDFKSFS
                     TPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYINGRIMHHGCLLFDVDLSVLAN
                     ALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYPEMTEYVFS
                     EEELAEINHIKDTKFGTWDWNYGKSPEFNVRRGTKFTSGKVEVFANVTESKIQDIKIY
                     GDFFGIEDVAAVEDVLRGVKYEREDVLKALKTIDITRYFVGISREEIAEAVVG"
     gene            complement(10492..11562)
                     /gene="xerS"
                     /locus_tag="ACL73L_001536"
     CDS             complement(10492..11562)
                     /gene="xerS"
                     /locus_tag="ACL73L_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HGS0772493.1"
                     /translation="MKREILLERIDKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLESGISNADKISDIPLSVLENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETAGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LRDLNLKMMVIDVTRKGGKRDSVNVAAFAKPYLENYLAIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVSDEQKNALDSL"
BASE COUNT         3558 a         2238 c         2672 g         3515 t
ORIGIN      
        1 gcttaatgat ttaaaaagta attataaaaa atagccagtc tttataaact gtattcacgt
       61 attcaaatcg tatatatagt cgatttatta aatctttaga taaactgtaa aaagtggttt
      121 ccatagccac tttttgctat aatagaggca gtaaaaatga aggagttggc tatggaacac
      181 tcgtcttggc acgctttgat taaggcgcaa ttacctgagg gttatttcgg gaaaatcaat
      241 cagtttatgg agcaggtcta ttctcagggg actatttatc cacccaagga aaaggttttt
      301 caggctctct tgacaacact gcttgaagaa gttaaggtgg taattctagg gcaagacccc
      361 taccacggac caggtcaagc gcagggcttg agtttttctg tacctgactc tatcccagct
      421 ccaccatcct tgcaaaatat cttgaaagaa ttgtcagatg atatcggagt taagaaatct
      481 catgatttga cagcttgggc tgagcaagga gtcttgcttc ttaatgcttg tttgactgtt
      541 cctgctggac aggccaatgg tcatgctggt caaatctggg agccttttac tgatgctgtg
      601 attcaggtgg tcaatcatct agatagacca gtcgtttttg tactctgggg agcttatgca
      661 cgtaagaaga aggcattagt taccaatcct catcacttga ttatcgaatc agcccatcca
      721 agtcctttgt cggtttatag aggattttgg ggttccaagc ccttttccaa ggccaatgca
      781 ttcttaaaag agacaggaca agagccaatc gattggctta gataaggaga aaatatgcct
      841 cagttagcga cgatttgcta cattgataat gggaaagaac tgctcatgct ccatcgtaat
      901 aagaaaccca atgatgtcca tgaagggaaa tggattggtg tgggtggtaa gctagagaga
      961 ggagaaacgc cccaggaatg cgcggtgcgt gaaatccttg aagaaacagg gctcaaagcc
     1021 aagccagttc taaaaggtgt catcactttt cctgaattta caccagattt agactggtac
     1081 acctatgttt ttaaggtgac ggagtttgag ggcgacttga ttgactgcaa tgaggggacg
     1141 ctagaatggg ttccctatga tgaggttttg agcaagccga cttgggaagg tgaccacacc
     1201 tttgttgagt ggcttttagg ggataaaccc ttcttttcag ccaagtttgt ttatgatggg
     1261 gataaattgt tggataccca agttgatttc tatgaataaa ggagaaagca gatgctacta
     1321 atcaaaaatg gtcgtgtaat ggatcctaag tctggtttgg atcaagtttg tgatgtctta
     1381 gttcaagatg ggaaaattat caaaattgcg cctgagatca cggaagaagg agcagaaacg
     1441 attgatgcta ctggtcttgt ggttgctcct ggtttggtcg atattcatgt gcatttccgt
     1501 gaacctggtc aaacacataa agaagacatt catactggtg ccctggcagc cgctgcaggt
     1561 ggttttacta ctgtcgtcat gatggctaat accagtccaa ccatttcaga cgtggagact
     1621 ttgcaagcag ttctccagtc agctgccaaa gagaagatta atgtcaagac agttgcgacc
     1681 attactaaaa actttaatgg taaaaacttg actgacttta aggcgctctt agaagctggt
     1741 gcggttggtt tctctgatga cggtattccg cttgagagca gtaagattgt caaggaagcc
     1801 atggaggaag ccaaaaaact caataccttt atcagccttc atgaggaaga tccaggtttg
     1861 aacggtgttc ttggctttaa tgaaaatatt gctagagaac atttccatat ctgcggtgct
     1921 actggggtgg ctgagtacgc tatgatggcg cgtgatgtca tgattgccta tgcaactaaa
     1981 gcccatgttc acatccagca tttgtctaag gaagaaagtg ttaaagtagt ggagtttgct
     2041 caggggttag gtgcagaagt cacagcagaa gtagcgccac agcatttctc taagacagaa
     2101 gcacttcttt taacacaagg tagcaatgct aagatgaatc caccgcttcg tttggaatca
     2161 gaccgtcgtg ccgttatcga aggtctcaaa tcaggtgtca tcacagttat tgcgactgac
     2221 cacgcgcctc atcatgtaga tgaaaaaaat gttgaggata ttaccaaagc gccatctggt
     2281 atgactggct tagaaacatc cctgtctctc ggcttgacct atttagtaga agctggtgag
     2341 ttgagcttga tggaattact tgaaaaaatg acatacaacc cagccaagct ttacaacttt
     2401 gaagcaggtt acttggctga gaatggtcca gcagatatca ctatttttga tgccaaggct
     2461 gaccgccttg tggactccca ttttgcttcc aaagcagcta attcaccatt catcggtgaa
     2521 accttaaaag ggcaggttaa atataccatc tgtaagggac aaatcgtcta tcaagcttga
     2581 taggagttag aatatgaatt attttcgaaa acgtagggag agacaagcga aatctaatag
     2641 tggaatttac tgtcccgcag ctaatcgttc caaaattcaa tatgaaacta aagaaaaagc
     2701 tgaccatgcg gttcaatatg ataacggagg tttagttaga agctattatt gtcgtacatg
     2761 tgcgtgttgg catactactt caaaatctaa taaacctccg ctaagtttga aaaactatgg
     2821 tctgaaactc tttggtttag ataaatcaga atagaaagag aagtatgtat aagacaaagt
     2881 gtttacgaga gaagttagta ttatttttaa aaattttctt cccaatcctg atctaccaat
     2941 ttgccaatta ttctgcctct tttgttgata ctgcaatgac aggtcaatac aacactatgg
     3001 acttggctgg tgtatctatg gcaaccagta tctggaatcc tttctttaca tttctaacag
     3061 ggattgtgtc agccttggtg cctatcattg gtcaccatct tggtcgaggc aaaaaggaag
     3121 aagttgcgtc tgatttttac caatttattt atttggcctt gggcctatct gtggtcttgc
     3181 tggggatggt acttttcttg gcaccaacaa tcttgaatca tattgggtta gaagcagcag
     3241 tagcggcagt agcggttcgc tatctttggt ttttatctat cgggattatc cccttgttgc
     3301 tctttagcgt cattcgttcc ttgctggatt cgctgggctt gaccaaactg tccatgtacc
     3361 tcatgctttt gttactccct ctcaatagcg gatttaacta tctcttgatt tacggtgcct
     3421 ttggtgttcc agaactggga ggggctggtg ctggtttagg aacatccttg gcctactggg
     3481 tcttgcttgg gatttctgtt ctggttttat ttaaacagga gaagctcaaa gccttacacc
     3541 ttgagaaacg aattccactt aatatggata acattaagga aggagttcgt ttaggtctgc
     3601 ctattggggg aactgttttc gcggaagtgg ctatcttttc agtggttggc ttgattatgg
     3661 ctaagttttc gtccttgatt atagctagtc accagtcagc tatgaacttt tcaagtctta
     3721 tgtacgcctt tcctatgagt atctcatcgg ctatggctat tgtcgtttcc tatgaagtgg
     3781 gagccaagcg atttgatgat gcgaaaacct atattggtct aggaagatgg actgccctca
     3841 tttttgcggc cttcacctta accttccttt acatttttag gggaaatgtg gccagtcttt
     3901 atggtaacga cccaaaattt atcgatttga cagtgcgttt tttaacttat agtcttttct
     3961 tccagttagc agataccttt gcggcgccgc ttcagggaat tttgcggggg tataaggata
     4021 cagttattcc tttttacctt ggtttgcttg gttattgggg cgtagcaatc cctgtggcta
     4081 cgctatttga ttctctaaca gattttggag cttattctta ctggatcggc ttgattatta
     4141 gtttgattgt gagtggggcg ctctaccgtt ggcgtttaac tgtgattatg aagagatttg
     4201 aatctttagc aaaatccaaa cgctaaaaag tttgtgaaaa aatattcttg ataagaaaat
     4261 cccttgtttt cacggggttt tcttttttat tttgtgaaat ttcttttaga aaaactttga
     4321 cagtatttgc caaaaacggt aaaatagtaa ggtaagaaga aagttagaaa ggcaagaaga
     4381 tgtcaacttt agataaaaat cttttgctag aaatgttccg taagatggaa gaaatccgtc
     4441 gcatggactt aaaaattgca caattagtaa agaaagggaa agtgccagga atgacgcact
     4501 tttctgttgg tgaagaggca gctaacgtgg gggctatgtt agctctcaat ccagatgatc
     4561 tgattacctc aaaccaccgt ggacacgggc aagctattgc taaagggatt gacctcaacg
     4621 gaatgatggc tgaaatcctt ggtaaataca ctggaacctg taaagggaaa ggtggatcta
     4681 tgcatatcgc tgaccttgat gctgggaacc ttggtgccaa tggtatcgta ggtggtggta
     4741 tggggatcgc tgtcggtgca gccctcagtc agcaaatgca aaataccggt aaaatcgttg
     4801 tttgcttctt tggagatggt gcgaccaatg aaggtgtttt ccacgaagca gtgaacatgg
     4861 cttctatctg gaacctgcca gtcattttct attgcattaa caacggttac ggtatctctg
     4921 cggatatcaa gaaaatgacc aatatagaac atatccatca acgtagcgcc gcttatggaa
     4981 ttcctggaat gttcatcgaa gacggtaaca atgtcatcga tgtctatgaa ggatttcaga
     5041 aagctgtaga ccatgttcgc agtggcaatg gtccagtctt gattgaaagt gtaacttatc
     5101 gctggctcgg tcactcatca tctgaccctg gtaaatatcg tacgcgtgaa gaagtggaat
     5161 tgtggaaaca aaaagatcca atcgaaaacc tccgcaatta ccttattgaa aataacattg
     5221 caagtgccga agaattggaa gaaatccaag cgcaagtaaa ggaagcagta gaagcttctg
     5281 ttaaatttgc agaggaaagc ccattcccac cgcttgaatc agcctttgaa gatatttacg
     5341 cagactaagg agaaagagat aaaatggaaa caaaaacaat gtccttccgt gacaccatta
     5401 tccttgctat gtctgaggaa atgcgtcgcg atgaaaatgt gttcttgatg ggagaagacg
     5461 tcggtgtctt cggaggagac ttcggtactt ctgttggaat gcttgaagaa tttggtccag
     5521 aacgcgtccg tgactgtccg atttctgaag ctgccatctc tggagcagca gcaggagcag
     5581 ccatgacagg acttcgtcca atcgtcgata tgaccttcat ggacttctct gttattgcca
     5641 tggacaatat cgtcaaccaa gctgctaaaa cacgttacat gtttggtggt aaaggtcagg
     5701 ttccaatgac tgttcgttgt gcagctggta acggagttgg ctctgccgcc cagcactcac
     5761 aatctctaga gtcttggttt actcacattc caggacttaa ggttgtggca cctggaacac
     5821 ctgcggacat gaaaggcctg cttaagtctt ctatccgtga taacaactca gttattatac
     5881 ttgagtacaa gtcagaattt aaccaaaaag gggaagtgcc agttgatcca gactacacaa
     5941 ttccacttgg ggttggtgaa atcaaacgcc aaggaacgga tgtaacagtt gttacttatg
     6001 gaaaaatgct tcgccgtgtg gttcaagctg ctgaagaatt agcagaagaa ggaatttcag
     6061 ttgaaattgt tgacccacgt acccttgttc cgcttgataa ggatatcatc ataaactcag
     6121 tgaagaaaac tggtaaggtt gttctggtca acgatgccca caaaacaagc ggctatatcg
     6181 gtgaaatttc agctattatt tcagaatcag aagcatttga ctatctagat gcaccaatcc
     6241 gccgttgtgc aggagaagat gtgccgatgc cttatgcaca aaacctagaa aatgcaatga
     6301 ttccaacagt tgaaagtatc aaagatgcaa tccgaaaaac ttataacaaa gaatagagtt
     6361 gcataagata agttatgtaa ataaaataaa gctgacgaga aactttgtat cttttaggtg
     6421 cagagctctt cgtcattaat atcttagatt agagcacggg ctaaaagctc ggaaaaaaga
     6481 taaatctcct tggcttcatc gaagccatcg tcgatttcct atttttcagt cgcttttgac
     6541 gccctttgta tcataaatag aattggagag gtcatggctg atgacaagct aagagcgact
     6601 cctgcagcta gaaagttagc agatgattta gggataaacc tctacgacgt ttctggctca
     6661 ggtgcaaacg gtcgtgtcca caaagaagac gtggaaactt ataaagacac aaacgtggtt
     6721 cgcatttcgc cacttgcaaa acgaattgcc ctcgaacata acattgcttg gcaggaaatc
     6781 caaggaaccg gtcatcgtgg taaaatcatg aagaaggatg ttttggccct gcttcctgaa
     6841 aatatcgaaa acgatagcat caaatctcct gctcagattg aaaaagtgga agaagtccct
     6901 gataacgtaa caccatatgg tgaaatcgag cgtattccaa tgacaccaat gcgtaaggtt
     6961 attgcccaac gcatggttga atcttacttg actgcgccaa ccttcaccct caactatgaa
     7021 gttgatatga ctgaaatgtt ggctcttcgt aagagggttc ttgagccaat catggaagca
     7081 actgggaaga agactactgt aacagacctt ctttcacttg cagttgttaa gactcttatg
     7141 aaacatccat gcatcaacgc ttcattgaca gaagatggca agactattat cactcacaac
     7201 tatgtcaatc ttgccatggc agttgggatg gataatggat tgatgacacc tgttgtttac
     7261 aatgctgaga agatgagtct ttcagaactg gttgtagcct ttaaggatgt tattggccgt
     7321 accttggatg gtaaattggc tccaagtgag ttacaaaatt caacattcac aatcagtaat
     7381 ttgggaatgt ttggtgttca gtcctttggt ccgattatta accaacccaa ctcagctatc
     7441 cttggtgtca gttcgacaat cgagaagcca gttgtcgtca atggtgaaat tgtgattcgc
     7501 ccaatcatga gtttaggatt aaccattgac caccgtgtcg tagatggtat ggctggtgct
     7561 aagtttatga aggacttgaa agagttgatt gaaacgccaa tctcaatgtt aatttaagag
     7621 caagagtatt catttttaag atatagataa aggaaggaag acatggcctt agaagtaatt
     7681 atgccaaaag ccggcgtgga tatgacagaa ggacaaatcg tccaatggaa taaaaaagtc
     7741 ggagaatttg taaaagaagg agaaatcctt ttggaaatca tgactgataa agtcagcatg
     7801 gaattggaag ccgaagaaga tgggtacttg attgccattc tcaaaggaga tggtgaaact
     7861 gtccctgtaa cggaagttat cggttacctt ggtgaagaaa gggaaaacat cccaacagct
     7921 ggagcagcat cgccagaagc tagccctgta cctgtagcaa gtacctcaaa cgatgatggt
     7981 aagagcgatg atgcttttga tatcgttgtg attggtggag gtcctgctgg ttatgttgca
     8041 gccattaaag ctgcccaact cggcggtaag gttgcccttg ttgagaaatc tgaacttggt
     8101 ggaacctgct taaaccgtgg atgtattcca accaagacct accttcataa cgctgaaatt
     8161 attgaaaata tcagtcatgc tgcaaatcgt ggtatcgtga ttgaaaatcc taacttcact
     8221 gttgatatgg aaaaactttt agaaactaaa tctaaagttg ttaatactct tgttggtggt
     8281 gttgcaggac ttcttcgtag ctacggagtt actgttcata aaggaattgg tacaatcact
     8341 aaagacaaga acgtcttggt aaatggttct gaattgcttg aaaccaagaa aatcattctt
     8401 gctggtggtt caaaagtcag caagatcaac gtccctggta tggaatctcc acttgtcatg
     8461 actagtgatg acattcttga aatgaacgaa gtgccagaaa gccttgttat catcggtggt
     8521 ggagttgtcg gtatcgaact cggtcaggcc ttcatgacat ttggttcaaa agtgactgtt
     8581 atcgaaatga tggaccgtat agttccagct atggatgcgg aagtttctaa gaaccttcgc
     8641 ttgatccttg agcgtaaagg aatgaccatc ttgactggta ctaaactgca agaaatcatt
     8701 gaggaaaatg gtcaacttcg tatcaaggtt gaaggaaaag acgatatcat cgcaagcaaa
     8761 gctcttcttt caattggtcg tatgccagac cttgaaggta ttggagaggt tgagtttgaa
     8821 ttggatcgtg gttgtatcaa ggtcaacgaa tacatggaaa cttcagttcc aggcatttat
     8881 gcaccaggtg acatcaacgg tactaagatg ttggctcacg cagctttccg catgggtgaa
     8941 gtttccgctg aaaatgccct taaaggaaat catgcagttg ccaaattgaa tttgactcct
     9001 gcagccatct acactctccc tgaagtagca gcagtaggtt tgacagaaga acaagcccgt
     9061 gagaaatacg atgttgccat cggtaagttt aactttgctg ctaacggtcg tgctattgca
     9121 tctgacgcag ctcaaggttt cgtaaaagtt atcgctgata agaaatatgg agaaatcctt
     9181 ggtgtacaca ttattggtcc tgcagccgca gaattaataa acgaagcatc aagcatcatc
     9241 gaaatggaaa tcactgttga ggaaatgctg aagaccatcc acgggcaccc aacttactct
     9301 gaagtgatgt acgaagcgtt tgcagatgtt ctaggaatgg ccatccattc acctaagaaa
     9361 aaataaaacg aagatagatt agactggaag gttatttcca gtctctatca tataaagtaa
     9421 agggatatca tgaaatacat tatcaatcat tcaaatgaca ctgcttttaa tattgccttg
     9481 gaagaatacg cctttaaaca ccttttggat gaggatcaaa tcttcctact ttggattaac
     9541 aagccatcta tcattgttgg tcgtcaccag aacactatcg aagaaatcaa ccgtgattat
     9601 gttcgcgaaa atggtattga ggtagttcgc cgtattagtg gtggtggagc tgtttaccac
     9661 gatttaaata acctcaacta cacgatcatc tccaaagaag atgaaaataa ggcatttgac
     9721 ttcaagagct tctcaactcc ggttatcaat accctggctc aactcggggt taaagctgag
     9781 ttcacaggtc gtaatgacct tgagattgat ggtaagaagt tctgtggcaa tgcccaagcc
     9841 tatatcaacg gccgtatcat gcaccacggt tgcttgctct ttgacgttga tttgtcagtc
     9901 ctcgcaaatg cccttaaggt ttcaaaagat aaatttgaat caaaaggtgt gaaatccgtc
     9961 cgtgcccgtg taactaatat tatcaatgaa ttaccaaaaa aaatcacagt cgaaaaattc
    10021 cgtgatttac tcttggaata catgaaaaaa gagtacccag agatgactga atacgtcttt
    10081 tcagaagaag aattggccga aatcaatcac atcaaggata ctaagtttgg aacttgggac
    10141 tggaactatg gtaaatcacc tgaatttaac gtccgtcgtg gaaccaaatt cactagtggt
    10201 aaggttgaag tctttgctaa cgttactgaa tcaaaaatcc aagacatcaa gatttatggt
    10261 gacttctttg gtattgaaga cgttgctgca gtagaagatg tccttcgtgg ggtgaaatac
    10321 gaacgcgaag atgttcttaa ggcgctaaaa accattgata tcacacgcta cttcgttggt
    10381 attagccgtg aagaaattgc tgaagcggta gttggataaa tcaaaaagaa gttggccaac
    10441 taaccaactt ctttttttga tatttattta cataatttaa aatacgtaaa atcataaact
    10501 atccagagca ttcttttgtt catcactaac aatatgggta tagaggtcag tgacttgtgt
    10561 gctagcatgt cctagttggt gactgactaa aacttgtgat ttagtcgcat catagagcct
    10621 agttgctagt gtatggcgca gtttatgggg tgttacacgc actttaaaat cctctgagta
    10681 tttagcaacc attttctcaa cgctagaagc atcgatacga ttaggaacac ctctgtagag
    10741 agttaaaaaa agggctgtat ctgttttttc cgttttatag cgttgattcc gaatggccag
    10801 ataattctct aaataaggtt tagcaaaagc agcgacattg actgagtcac gtttgccacc
    10861 ttttcgagta acatcaataa ccatcatttt tagattgaga tctcttagat ctagattaac
    10921 agcttcagat aagcgaacac cagatgccaa gagaagggca ataatggcta aatctcgttc
    10981 tttatttttg ttgaatgatg agagagctcg atttgaaagc tgttgtgggt gctcttgatc
    11041 gatataagtt agaaaacctg ctgtttcatc acctagaaag agtttttgct tgatattttc
    11101 agctctggca gcaagggttt ctttcttttt cttggttgaa acttttttca ttacattacg
    11161 atagaaataa ggttccccct gatcgttttc aacctcctcg gttagatact tgtaaagact
    11221 agaaagtgct gataaggttc gattgatagt tgtctgtgaa acaccttgtt ttgttgtatt
    11281 agcattcagc aagggacgtt cacgtagata aaggataaag gattccatgt ctttcttaga
    11341 catattttcc aaaactgata aaggaatatc ggatatttta tcagcgtttg aaataccaga
    11401 ctccaaaacc cagctgaaaa atcggtcata ttccttaaga tattcgtaca gggttgtaaa
    11461 actgtagggc acagccagct tagattggta gtattccaga acataccagg gcatgagttg
    11521 ttttagtttg tcgattcgtt ccagtaaaat ctcacgtttc atgtattttc tccataatta
    11581 agtatactag tagtatagca tagacttgta tgtttttcaa gaaaattata gtttttcaga
    11641 aagatgttat aggagttttt caaagtgata aaaagactta gacctacaaa caaaacagaa
    11701 taaggaaaat aataaaaatc tcacccacta aacttctgtt aatactacat aactgaagag
    11761 ctatataagg agctatgaga tacaaaacac attgtataat agattgaaac tagaatagta
    11821 catctggact tctaaaacat tgctagaaat tgatttgatt gtcctgattg ttttatccta
    11881 tttctatttc atttctctat ataattgcta ttgttttctt tttcatgatt ttaatcttac
    11941 tgtacagatt tgatacagta aactcctttc tagctttata ctt
//
LOCUS       DBERDK010000055        11682 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.055, whole genome shotgun sequence.
ACCESSION   DBERDK010000055 DBERDK010000000
VERSION     DBERDK010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 11682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11682
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.055"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(73..534)
                     /locus_tag="ACL73L_001537"
     CDS             complement(73..534)
                     /locus_tag="ACL73L_001537"
                     /inference="COORDINATES: protein motif:HMM:NF013842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 transposase-related protein"
                     /protein_id="HGS0772494.1"
                     /translation="MGKERREQGNLERKKRVVKKRKIPLEELKAFVEAHPDAFLREIA
                     ARFDCALPSVWAVLKQIKVILKKTTSFREQKPEKVSEFLDILDNLKDLPVLYIDETGI
                     NRYLYRPYAGAPRGEKVYDKISGRRFERTNEVEQKLNGSFLIRYIDSQIRE"
     gene            complement(501..644)
                     /locus_tag="ACL73L_001538"
     CDS             complement(501..644)
                     /locus_tag="ACL73L_001538"
                     /inference="COORDINATES: protein motif:HMM:NF013842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 transposase-related protein"
                     /protein_id="HGS0772495.1"
                     /translation="MAYSTDFKQGALDSIKGGHRHVEAAKVFDVGVRTLFTWEKKDVS
                     KGT"
     gene            912..1292
                     /locus_tag="ACL73L_001539"
     CDS             912..1292
                     /locus_tag="ACL73L_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HGS0772496.1"
                     /translation="MTYLEKWFDFNRRQKEIESLLEETIAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRMCCHQDRRSSFICLTND
                     GQKTLASLQKTVEESLEAGLDFLI"
     gene            1392..1613
                     /locus_tag="ACL73L_001540"
     CDS             1392..1613
                     /locus_tag="ACL73L_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HGS0772497.1"
                     /translation="MIEITYIDASKNERTVTFESYEDFERSQQACLIGVADYYPVQKL
                     TYKGHNLDYHGTYGDIFFYLMKQDLSQYN"
     gene            1630..1944
                     /gene="trxA"
                     /locus_tag="ACL73L_001541"
     CDS             1630..1944
                     /gene="trxA"
                     /locus_tag="ACL73L_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HGS0772498.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTAEQIKAIIAE
                     LS"
     gene            2249..2782
                     /gene="queF"
                     /locus_tag="ACL73L_001542"
     CDS             2249..2782
                     /gene="queF"
                     /locus_tag="ACL73L_001542"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046857 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors, with NAD or NADP
                     as acceptor [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HGS0772499.1"
                     /translation="MKHQSALVVFSGGQDSTTCLFWAMQHYETVEAVTFAYGQRHHLE
                     IQITREIAKEQGIRHHILDMSLLGQITAQPDFATIHISYIPDKLCVESKSLKLYLFSY
                     RNHGDFHENCINTIGKDLVNLLDPRYLEVWGKFTPRGGISIDPYYNYGKQGTKYEGLA
                     EQRLFQHDLYPEKIDNR"
     gene            2794..2967
                     /locus_tag="ACL73L_001543"
     CDS             2794..2967
                     /locus_tag="ACL73L_001543"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772500.1"
                     /translation="MKIKEQTRKLAAGCSKHCFEVADRTDEVSNHTYGKVKLTWFEEI
                     FEEYHTKKPCSSR"
     gene            complement(2992..3660)
                     /locus_tag="ACL73L_001544"
     CDS             complement(2992..3660)
                     /locus_tag="ACL73L_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HGS0772501.1"
                     /translation="MKKFVAELIGTFMLVFVGTGAVVFGNGLDGLGHLGIAFAFGLAI
                     VVAAYSIGTVSGAHLNPTVSIAMFVNKRLSSSELVNYILGQVVGAFIASGAVFFLLAN
                     SGMSTASLGENALANGVTVFGGFLFEVIATFLFVLVIMTVTSESKGNGAIAGLVIGLS
                     LMAMILVGLKITGLSVNPARSLAPAVLVGGAALQQVWIFILAPIAGGVLAALVAKNFL
                     GTEE"
     gene            complement(3771..4562)
                     /locus_tag="ACL73L_001545"
     CDS             complement(3771..4562)
                     /locus_tag="ACL73L_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HGS0772502.1"
                     /translation="MYQDLLRKIAEEKPNYNQEEIQWLFDHLGNPSPEIRDDLVFTSF
                     ARGIQEELFTQEQFHFIAEVVLADGGLDKEIDKVGLSTLERSFRALIYANLLSADANQ
                     QSVFYQELNAGFRNVLLNQGLHYLSKEKDTTGFSSQYGWVHAFAHGADLLTEVVCHPD
                     FPKNRVHEVFDILGQLFKRMSIRFTDDEDWRLARVIYEPILQGKLEQEQVASWIKTVD
                     FPIEEREDFYKFSNFRSCLVEVYVQLDQRNSLQDELKEAIQSFQY"
     gene            complement(4808..6604)
                     /gene="pepF"
                     /locus_tag="ACL73L_001546"
     CDS             complement(4808..6604)
                     /gene="pepF"
                     /locus_tag="ACL73L_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HGS0772503.1"
                     /translation="MEQKHRSEFPEKELWDLTALYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHTFEDFEKAFAELEQIYIQMSHIGNYGFMPQTTDYSNDEFANIAQAGMEFETD
                     ASVALTFFDDALVAADEEVLDRLGKLPHLTAAIRQAKIKKAHYLGADVEKALTNLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFVTYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLADMKGYDSVFDYLLAEQEVDRAMFDRQIDLIMKDFA
                     PVAQRYLKHVAKVNGLEKMTFADWKLDLDSALNPEVTIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEERWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDATLTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLHLKHSETGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIISYSAELGE"
     gene            complement(6615..7358)
                     /locus_tag="ACL73L_001547"
     CDS             complement(6615..7358)
                     /locus_tag="ACL73L_001547"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HGS0772504.1"
                     /translation="MQQYFVKGSAVSPVTIEDRETSKHMFQVMRLKEEDEVTLVFDDG
                     IKRLARVLDMENRQFELVQELNDNVELPVQVTIASGFPKGDKLEFIAQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLAQLDQ
                     FDSIIVAYEESAKEGEAAALLQAVTGLETGAKLLFIFGPEGGLSPAEIESFEAKGAVL
                     AGLGPRILRAETAPLYALSALSVLVELEK"
     gene            complement(7360..8310)
                     /gene="prmA"
                     /locus_tag="ACL73L_001548"
     CDS             complement(7360..8310)
                     /gene="prmA"
                     /locus_tag="ACL73L_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HGS0772505.1"
                     /translation="METWQELKVTVKREGEELVSNLLIELGAQGVAIEDSMDYVGNVD
                     RFGEIFPEVEQQEEIVVTAYYPDTVDVTVVEADLQARLAELTDFMDLGELKIGTTALA
                     EEDWADNWKKYYEPARITHDLTIVPFWTDYEATAGEMIIKLDPGMAFGTGTHPTTKMS
                     LFALEQVLRGGETVLDVGTGSGVLSIASSLLGAKEIFAYDLDDVAVRVAQENIELNTG
                     MENIHVAAGDLLKGVEIEADVIVANILADILIHLTEDAYRLVKDEGYLIMSGIIKDKW
                     DMVRQSAESAGFFLETHMIQGEWNACVFKKTKDISGVIGG"
     gene            complement(8446..8874)
                     /locus_tag="ACL73L_001549"
     CDS             complement(8446..8874)
                     /locus_tag="ACL73L_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HGS0772506.1"
                     /translation="MELEISDFLGCKIALFCGDKLLTILRDDKASIPWANMWELPGGG
                     REGDESPFECVAREVYEELGIHLTEDCLLWSKIYPSMLFADKQSVFLVGQLTQNQFDS
                     IVFGDEGQGYQLMNVEEFLSSSQVVPQLQERLKDYLKVSD"
     gene            complement(8855..9943)
                     /locus_tag="ACL73L_001550"
     CDS             complement(8855..9943)
                     /locus_tag="ACL73L_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HGS0772507.1"
                     /translation="MKKIGTYLVYVLAVVFIMLAFACGTIAFAELGYSAVLAFTFGYA
                     FALLSMYLIFILHELGHAFCGYLTGYRLVAFGLGHFILTKKSGRFHLSRTAILKNVGA
                     QYIGLKEDESDQRIILMLSGGLMVHLGLILLAIVFGFLTRSWYFAGTWIFLNLSFFLN
                     NILPVDITDGAKIWELLQHPENTKYAYLVLRHSAQTLLVPQEYDLKDFVQAVPEDARG
                     SFADGVLGMQGEVSILEGKEEVAKQQFQALLERTTTPMMQTVAQLSLLHIALLEGDFE
                     QAEQYASIRQVKSFLSLKLSNLQVIQAWYQLKVKKDVDRTRKALKIARQKMDSSRMLR
                     DEKRYYENLLAELEKEMLEGTEDGTRNF"
     gene            complement(9962..10432)
                     /locus_tag="ACL73L_001551"
     CDS             complement(9962..10432)
                     /locus_tag="ACL73L_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HGS0772508.1"
                     /translation="MATYGFLDILEEELDKNFPFDFEISWDKRNHAVEVSFLLEAQNA
                     AGVEMVDEDGEVSSDDILFEEAVLFYNPAKSTVNEEDYLTVIPYLPKKGFSREFLAYF
                     ALFLKDTAEVGLDVLMDFLEDPEAEEFVMEWNQEVFEEGKVGLEEGEFYPYPRY"
     gene            complement(10723..11175)
                     /locus_tag="ACL73L_001552"
     CDS             complement(10723..11175)
                     /locus_tag="ACL73L_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HGS0772509.1"
                     /translation="MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAM
                     GSEWLGITVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA"
     gene            complement(11212..11391)
                     /locus_tag="ACL73L_001553"
                     /pseudo
     CDS             complement(11212..11391)
                     /locus_tag="ACL73L_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397531.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
BASE COUNT         3487 a         2717 c         2118 g         3360 t
ORIGIN      
        1 taaagacacg caaatgagta taaatcagct aatttttggt ttacattcct atttttatgc
       61 ataattattc tgtcattctc taatttgtga atcaatatat ctgattagaa aactaccatt
      121 gagtttttgc tccacctcat tagtccgctc aaaacgccgt ccgctaatct tgtcatagac
      181 tttctcccct ctaggagccc ctgcataagg acgatagagg tagcggttga ttcccgtttc
      241 gtcaatatat aggactggta aatcttttag gttatccaaa atatcaagaa actcagaaac
      301 tttctcaggc ttttgttccc taaaactggt cgtctttttt aaaatgacct taatctgctt
      361 taaaactgcc catacggagg gcaaagcaca atcaaaacgg gccgcaattt cccgtaaaaa
      421 agcgtctgga tgagcctcta caaaggcttt caattcttct aaagggatct tacgcttttt
      481 gacgactcgc tttttccgct ctaagttccc ttgctcacgt ctttcttttc ccacgtgaag
      541 agagttctga cgccgacatc aaaaactttg gccgcctcga catgtctgtg ccctcctttg
      601 atggaatcta atgctccttg tttaaaatca gtactatatg ccatagcttt attagaccat
      661 gcttatttta aactaagcaa ctatagatag agaaatgaaa taaaatctga attcgaatgt
      721 gataaggagt tagaaattga aacaatagga ttcccccatt attcacacaa aataaaacca
      781 ctaaaaaaca gagcttgcca gcattcttga aaagtagtat aattattgca tgcgcaatca
      841 tcttgtgacg cagagactgc ccgttaatgg actttcttct atattatgac tctaaaaaga
      901 aaggagacac tatgacctat ttggaaaaat ggtttgactt caatcggcgt cagaaagaaa
      961 ttgaaagtct cttggaagag accattgccc aacagagcga gcaaagtctg accttgaaag
     1021 agttctacct gctctactat ctggacttgg ctgaagaaaa atctctacgc cagattgacc
     1081 tgccagataa gcttcatctg agcccgagcg cagtttctag gatggtggct cgcttggaag
     1141 ctaaaaattg cggtctactc agtcgcatgt gttgccatca agatagacgc tctagtttta
     1201 tctgcctgac aaatgatggg caaaagacac tggcctcgct acaaaaaact gtcgaagaaa
     1261 gcttggaagc aggtttggat ttcttgattt aagatgattt ttaaaaaaag ttgcgtgcgc
     1321 aatcattttt cttgacattc tcttttataa ggagtaaaat aaagttagca ttaaacaaag
     1381 gagttttaaa tatgattgaa attacctata tagatgccag caagaacgaa agaactgtga
     1441 ctttcgagtc ttatgaagac tttgaacgtt cgcaacaagc ttgccttatc ggcgtcgcag
     1501 actactaccc tgtccaaaaa ttaacttaca agggtcataa tttggactac catgggactt
     1561 atggagatat cttcttctat ctcatgaaac aagatttaag ccaatataac taaaaaaagg
     1621 agaaatacaa tggcaaaagc aattacagat gcaacattcg aacaagaaac aaaagatggt
     1681 ttggtcttag tagacttctg ggcaacttgg tgtggtccat gtcgtatgca aggtccaatc
     1741 ttggacaaat tgtctgaaga actttcagaa gatgtcttga aaatcgttaa aatggacgtt
     1801 gatgaaaatc caaacacagc tcgtgctttt ggaatcatgt ctattccaac tcttctcttc
     1861 aaaaaagacg gccaagttgt caaacaagtt gcaggtgttc acacagcaga acaaatcaag
     1921 gccatcattg ctgaattgag ctaatcaaac gagagaccaa gtactttctt tggtctcttt
     1981 tttcttgccc tttgccattt ttcaaaaaat atgctagact atagtagatt gaaactagaa
     2041 tagtacacct ctacttctaa aacattgtta gaaatcgatt tgactgtcct gatcgatttg
     2101 tcctgttctt atttcatttt actatataag tagaatatta cgatgtttgg gatatgccat
     2161 cgctaaaaaa actcgatttg gtatttttta gctccctaat gggagctttt tgcgtgctct
     2221 gaacatttcc cattttggaa ggagtactat gaaacatcaa tcagccttgg tcgtctttag
     2281 tggtggtcaa gattcaacaa cctgcctttt ctgggccatg caacactatg aaacagtcga
     2341 agctgtcacc tttgcctacg gccaacgtca tcacctcgaa attcaaatta ctagagaaat
     2401 cgctaaggaa cagggcattc gtcaccatat cctcgatatg tctctgctgg gacaaatcac
     2461 tgctcagcca gactttgcga cgattcatat ttcctacatt cctgacaagc tctgtgtcga
     2521 gtcaaaatcc ctcaaactat atctatttag ctaccgaaac cacggagatt tccacgaaaa
     2581 ctgtatcaac accatcggga aagacttggt caacttgcta gaccctcgct atttagaagt
     2641 ctggggaaaa ttcactccgc gcggtggcat ttcaatcgac ccctactaca actacggtaa
     2701 gcaaggaact aagtatgagg gcttggcaga acaacgcctc ttccaacacg acctttatcc
     2761 agagaaaatt gacaaccgct aaagtcatac tcaatgaaaa tcaaagagca aactaggaag
     2821 ttagccgcag gttgctcaaa acactgtttt gaggttgcag atagaactga cgaagtcagt
     2881 aaccatacct acggcaaggt gaagctgacg tggtttgaag agattttcga agagtatcat
     2941 acgaaaaagc cctgttcctc aagatgagga gcaaggcttt ttgagtttca attattcttc
     3001 tgttccaagg aaattttttg caacaagggc tgcaagaact ccaccagcga ttggtgcaag
     3061 gatgaaaatc caaacttgtt gaagggctgc gccgcctacc aagacagctg gtgccaagct
     3121 acgagctggg tttactgaaa gtccagtaat cttcaatccg acaagaatca tcgccatcaa
     3181 tgacaaaccg attaccaaac cagcaatcgc gccattgccc ttgctttctg aagtcacagt
     3241 catgataacc aatacaaaca agaaagttgc gatgacttca aacaagaaac caccaaagac
     3301 agtgacaccg tttgccaagg cattttcacc aagactagca gttgacatac ctgagttagc
     3361 caagaggaag aagacagcgc cagaagcgat gaaagctcca acaacctgac caaggatgta
     3421 gtttacaagt tctgaagatg acaaacgttt gtttacaaac atagcaatcg aaacagtcgg
     3481 gttcaagtga gcacctgaaa cagttccgat tgagtaggct gccaccacga ttgccaaacc
     3541 aaaggcaaag gcgattccaa ggtgaccaag gccatcaaga ccatttccaa aaacaacagc
     3601 tcctgtccca acgaacacaa gcatgaacgt accgattaac tcagcaacaa attttttcat
     3661 tttctttctc cttttttcaa aaactagata ctagtctatc aaaagtagga aagggtttca
     3721 agaaaattga ttggaaattt tttgaaaatc atagaactat tagctaatct ctagtattga
     3781 aaagactgga tagcttcttt caactcatct tgtaaactat ttctctggtc aagttggaca
     3841 tagacttcca ccagacagga tctaaagttg gaaaatttgt aaaaatcctc cctttcttct
     3901 atcggaaaat caacagtttt tatccaagaa gctacttgtt cttgctccaa cttcccttgt
     3961 aaaataggtt catagatcac tcttgctaaa cgccaatcct catcatctgt aaagcgaatc
     4021 gacattcttt taaatagttg gccaagtata tcaaatactt catgaactct gtttttagga
     4081 aagtctggat gacaaaccac ctctgtcagt aaatcggctc catgtgcaaa agcgtgaacc
     4141 caaccatact gacttgagaa acctgttgta tccttttctt ttgaaagata gtgcaagcct
     4201 tgatttaaaa ggacattacg aaatcctgca tttaattcct gataaaaaac cgattgctgg
     4261 ttggcatctg cagacaagag atttgcataa ataagtgccc taaaagaacg ttcaagtgtt
     4321 gacaagccta ccttatcaat ctctttatct aaccctccat cagctaagac cacctcagca
     4381 atgaaatgaa attgctcctg tgtaaatagc tcttcctgaa tccctctagc aaagcttgta
     4441 aaaacaaggt catcacgaat ttctggagaa ggatttccca aatgatcaaa caaccactgg
     4501 atttcttcct ggttataatt tggtttttct tctgctattt ttcttagtaa atcttgatac
     4561 atggtcaata cctctacatt tctagcaact gttcaaaaag gcagtcttaa atgactcaat
     4621 attgaattct caattaaata caatctgata taaaatgacg taaataacta tcaataccag
     4681 ttctacagta agttcaaatt taacatcacg accttcaacg acatttttga aaatagctac
     4741 aactaagaca aatagaatga cgcttaacaa gcccataaac atcattctaa aaaatttttc
     4801 tattccccta ctctcccaac tcagcactat aggagataat ctggtcaact gtgtcagaca
     4861 agaattggat ggtatcacgg agtggtttgt ctgttgaaat atcagcaccg ataatcatgg
     4921 ctgactcaag tggtgtcttg ctaccacctg atttgaggag attgagccag tcttcagctc
     4981 cagtttcaga atgttttaga tgaaggtaac cagcagtcga gataactagt cctgctgagt
     5041 aagtgtaact atacaagccc atatagtagt gagcttggcg catccaagtc agagttgcat
     5101 catcgtcaat ttcaatagca tctccccaga aatccgtcaa aacttccttc ataatgctgt
     5161 tgagcttgct tgctccaaag gtctcccctt cttcaatcaa tgtatacacc ttacgctgga
     5221 aggcggcttc caagagatgg gtgataaagt tatggaagta ggtgtctgtc aagcgatgag
     5281 ccagagcgaa gcgtttttga cgtgggtcat tagactggtt ctccaagtaa tcactgagta
     5341 gcaattcatt gaaggttgac ggtgcttcaa catagtaggt cgacatatgg gcattgaagt
     5401 aactttgatg attgtctgaa aagatgaatt gaccagaatg cccgatttca tgaatcaagg
     5461 tatagacatc gctcaaacgg cctgtccagc tcatgagtac ataagggtgt acgcgatatg
     5521 ggtccgccgc ataaccaccg gaatccttgc cactgttagc agcaaagtcc acccagcgct
     5581 cttcttggta acgagcaact tcctgacaat attcttgccc caaaggttct accgacttca
     5641 tgaccaaatc ataggcatcg tcaatagtca cttcaggatt cagggcgctg tccaagtcca
     5701 atttccagtc tgcaaaggtc atcttttcaa gaccatttac cttggcaaca tgcttgaggt
     5761 atctctgagc gactggtgca aagtccttca tgatgaggtc aatctggcgg tcaaacatgg
     5821 cacggtccac ttcttgttca gctagaagat agtcaaagac agagtcgtat cccttcatat
     5881 cagccaagag tttttcagac ttgacctgag ccagataggc tgctgcagcc gtattttggt
     5941 gcttacgaag tccctctgag aaggaacgga aggatttctc acgaacctca gcatcctcat
     6001 ggttttggta gaaattctca taggtcacaa agctgttttt gtaggtcttg ccatgggctt
     6061 caaagtcagc catttcaaaa tccccagctc gcatcttagt ataaatgtcc tgcggactgt
     6121 agaaaacttc accgagattt gtcaaggcct tctccacatc tgcccctaag tagtgggctt
     6181 ttttgatttt agcctgacga atggcagctg ttaaatgtgg caatttaccc aaacggtcca
     6241 agacttcctc atctgctgcc accaaggcat cgtcaaagaa ggtcaaggct acgctggcat
     6301 ctgtttcaaa ttccatccca gcttgggcaa tattggcaaa ttcgtcattg ctatagtccg
     6361 tcgtctgagg cataaaacca tagttgccaa tatggctcat ctgaatgtag atctgttcca
     6421 attccgcaaa ggccttctcg aaatcctcaa aagtgtgaag attgcccttg taatcacggc
     6481 taaactggtt gatgtcttcg cgagctttct cgattgcacg caagaaatcc tcacggtctt
     6541 ggtatagggc tgttaagtcc cagagttcct tctctggaaa ttctgaacgg tgtttttgtt
     6601 ccattttctt cctcttattt ctctaattct actaaaacac taagggctga taaagcgtaa
     6661 agcggtgctg tttctgctcg caaaatacga ggacctaggc ctgccaaaac ggctccttta
     6721 gcttcaaaac tttcgatttc tgcaggtgag agaccgcctt ctggaccaaa gataaagagc
     6781 agtttggctc ctgtttcaag accagtgact gcttgcagaa gcgcagcggc ttctccttct
     6841 ttagctgatt cttcataggc tactatgata gagtcaaact ggtccagctg agctagaaaa
     6901 tctgcttttt tctcgaaaag tttaatactt ggtacaatat tacgcttgct ttgctcggct
     6961 gctccaaggg caattttttc tagtttttca acttttttac ccaatttctt gccatcccac
     7021 ttggcaactg accagtctgc aggaaaggcc cagatttggc tagcccccag ttcggttact
     7081 ttttgagcga tgaactccag cttgtctccc ttgggaaatc cagatgcgat ggtcacttgg
     7141 actggtagtt ccacattgtc atttaattct tggaccaact caaactgacg attttccata
     7201 tccagcacgc gcgccaagcg cttgatgcca tcatcaaaga ctaaggtaac ctcatcctct
     7261 tctttcaagc gcataacctg aaacatatgc ttgctggttt ccctgtcctc gatggtgaca
     7321 ggagagactg cactcccctt gacaaaatac tgttgcatgc tagcctccaa tcacacctga
     7381 aatatccttg gtcttcttga agacgcaggc attccattcc ccttgaatca tatgggtttc
     7441 aaggaaaaat ccagctgact cagccgactg gcgcaccatg tcccacttgt ccttgataat
     7501 gccactcatg atgaggtagc cttcatcctt taccaagcga taagcatcct ccgtcagatg
     7561 aatgagaata tccgccaaga tattagccac gatgacatct gcttcaatct caactccctt
     7621 aagcaagtct cctgctgcta catggatatt ttccatacca gtgttgagct caatattttc
     7681 ctgagcgaca cgaaccgcca catcatccaa gtcataggcg aaaatctcct tggcacctag
     7741 gagtgagctt gcaatagaaa gaacccctga accagtccct acatctagca ctgtttcgcc
     7801 accacgaaga acctgttcca aggcaaagag gctcatcttg gttgttgggt gggtccctgt
     7861 cccaaaggcc ataccaggat ccagcttgat aatcatttcc cccgcagtcg cctcatagtc
     7921 tgtccaaaag ggaacgatgg tcaaatcatg agtgatacga gctggctcat agtatttctt
     7981 ccagttgtct gcccagtctt cctcagccaa ggcagtcgta cctattttta actctcccaa
     8041 atccataaaa tctgtcaatt ctgctagacg agcctgcaaa tccgcctcaa ccactgtcac
     8101 atccaccgtg tcagggtagt aggctgtcac tacgatttct tcttgctgct ccacctctgg
     8161 gaaaatctct ccaaagcggt ccacatttcc cacatagtcc atactgtctt cgattgcgac
     8221 tccttgcgct cccagctcaa tcaagagatt ggaaaccaac tcctctccct cacgcttcac
     8281 tgtaactttt aactcttgcc atgtttccat tattaatacc aagcccgtaa aacacaaaac
     8341 caaaatagga aattctctga agacgcttgt gtctaagaga agtttatctt tttggcacag
     8401 tgtttagggc gggttcagtt tagaaatgta actgaaccat cctttctaat cacttacttt
     8461 taaataatct tttaatctct cttgcaactg aggcacaact tgactggaac taagaaattc
     8521 ctcaacattc atcagctgat agccctgtcc ttcatctcca aatacgatac tgtcaaactg
     8581 gttctgtgtc aactgaccaa ctagaaaaac ggactgtttg tctgcaaaaa gcatacttgg
     8641 gtaaatctta ctccaaagca gacaatcttc agtcagatga atgcccagtt cttcataaac
     8701 ttcacgcgct acacactcaa aaggactttc atcaccttca cgtccaccac ctggcagttc
     8761 ccacatattg gcccagggaa tgcttgcctt atcatcgcgt aagatagtca aaagcttatc
     8821 cccacaaaac aaagcaatct tacagcctag gaaatcagaa atttctagtt ccatcttcag
     8881 ttccttctaa catttccttt tccagctctg ctaacaagtt ttcataatag cgtttctcgt
     8941 ccctcaacat tcgactacta tccattttct gtctagcaat cttgagagcc ttacgagttc
     9001 gatctacatc tttcttcacc tttaattgat accaggcttg tatcacttga agattggaca
     9061 gtttgagaga cagaaacgat ttgacctgtc gaatactagc atattgctcc gcttgctcaa
     9121 aatctccttc caacaaggcg atatgaagca gggatagttg ggcaactgtc tgcatcatcg
     9181 gagtagttgt cctctcaagt aatgcttgaa actgctgttt agctacttct tccttccctt
     9241 ccaaaatgga aacttcacct tgcataccta atacaccatc cgcaaaactc cctcgtgcat
     9301 cctcaggaac tgcttgaaca aagtctttca aatcatattc ttgaggaacc agcaaagttt
     9361 gagcagaatg tctcaatacc aggtaggcgt atttggtatt ttcagggtgt tgtagtaatt
     9421 cccaaatttt tgctccatcg gtgatgtcga ctggcaaaat gttatttagg aagaaagata
     9481 aattaagaaa aatccaagtc cctgcaaaat accagcttct tgtcaaaaat ccaaacacta
     9541 tcgccaataa tatcaagccg agatgaacca tcaagcctcc tgaaagcatc aggatgattc
     9601 tttgatcgct ttcatcctct tttaaaccaa tgtattgagc accaacattt ttcagaatgg
     9661 ctgttctact aagatgaaac ctgcctgact ttttggtcaa aataaaatgt cctaatccaa
     9721 aagccaccag ccgatagcct gtcaagtagc cacaaaaagc atgacccagc tcatgaagaa
     9781 taaagattaa atacatgctt agaagagcga aggcataacc aaaagtaaag gctaaaactg
     9841 cggaataccc caactctgca aatgcgattg ttccacaagc aaaagctagc ataataaaga
     9901 caacagctag cacataaacc aaataagtcc caattttctt cataacacct ccaaccaact
     9961 cctagtatct tggataaggg taaaattctc cctcttccaa gccaactttt ccttcttcaa
    10021 agacttcttg gttccattcc atgacaaatt cctctgcttc tgggtcttcc aaaaagtcca
    10081 tgaggacatc tagcccaacc tcagcagtat ctttaaggaa aagcgcaaaa taagctaaaa
    10141 attcacggga aaatcctttt ttaggcaggt aaggaataac agtcaaatag tcttcctcat
    10201 tgactgttga cttggcagga ttgtagaaaa ggaccgcttc ctcaaaaaga atgtcatctg
    10261 atgaaacctc tccgtcttca tccaccatct ccacacctgc agcattttgc gcttccaata
    10321 gaaaactcac ttctaccgca tggttgcgtt tgtcccaact aatctcaaag tcaaagggaa
    10381 agttcttgtc caactcttcc tctaaaatat ctaaaaatcc gtatgttgcc attttgtcct
    10441 ctttctatgc gactctttaa tcgccccgat tgctcggaaa tatgctaaaa tagatactac
    10501 catcttacca caaaattatt ttatgtccta attataccat attacctcat ttaaaccctt
    10561 ggtatcagtg attttcttaa aagtctgatt tcttcatttc tcataaaaat caatataaaa
    10621 agccctcgaa agggctaata aatctataaa atcaataggc gagtaactag cacaagtgga
    10681 cgtgcttttt tattgactat taccacgata ccacgcttaa tcttaggctt gaactttctt
    10741 atctgcaata gcgtctgtca aagtctgaga aaagttaagt cccatttctc gtcccaactt
    10801 atctgcccat tttggtatgg tcaaagtctt tttaatgggt tcctgacttc ctaggtattc
    10861 tgatacatca acagatacca tagaaataaa agatttatca aggtcatagg ttgacacgaa
    10921 atcttcatca tctttaaaag gatcattatc aattaaagac aagctattga tatctgatgg
    10981 ctgaggtaac tctccatcac tctctatcaa atctgcaaca gttatcccta gccactccga
    11041 ccccatagcc aaagcctcag aaatcccctc tccttgtgta gctgagtatt caaaatctgg
    11101 gaaatggaca aaataagtcg cttctgttcc gtctgtgtcg tcataataaa ataaagctgg
    11161 atacgtaact aacatttcac tacctccata tcaaaaagca ggggctgaat tttacaaccc
    11221 agcttgcttt cttatccctc tttcagtgta cttattcagc ttaccatgaa ggattgtgat
    11281 aggtctttcc ccttactttt ccattttaat atgggagcct ttaccgcctc tagtctttat
    11341 ccaaccatgg gccgtaagga gtttaaccat ctctttttgt gtcataggca tagcgctttt
    11401 acctcctgac aacaccatta taacacgtgt tacacgtatt gtaaaggagt gatacttatt
    11461 attctattat acataaaagc ccctagatgt ggttctaagg gaagccaatt tattcatacc
    11521 tatttttcta atgagtagta aaaactgctt ctttatcgag caattcatca tctgtatagt
    11581 caattgtaaa agtatctcga tctaagacag attgaggcgg agttgaatga atcataggaa
    11641 cactgcgtac tctatatttt ttatctccaa tttttacaaa ct
//
LOCUS       DBERDK010000056        11359 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.056, whole genome shotgun sequence.
ACCESSION   DBERDK010000056 DBERDK010000000
VERSION     DBERDK010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 11359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11359
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.056"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            8..1686
                     /locus_tag="ACL73L_001554"
                     /pseudo
     CDS             8..1686
                     /locus_tag="ACL73L_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031864.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            1954..2520
                     /locus_tag="ACL73L_001555"
     CDS             1954..2520
                     /locus_tag="ACL73L_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772510.1"
                     /translation="MVILELYQNDYSKDLVAFDSIEDGKAFVAQIPGYTLETEDGFEV
                     EYFNPKNIPDYMEIIFNGNIVPLSKFMFDPGENVNIIWKEISNLSLKNDRVIEGYSKI
                     DAYVVNNHEVKAYVETRETNYRKAKDFLESRGYEIDRSFFGSEDGEAILYRKKGIEVW
                     HFLCHLDPMFVEIEDVEGYVKEEVGEIQ"
     gene            2843..3031
                     /locus_tag="ACL73L_001556"
     CDS             2843..3031
                     /locus_tag="ACL73L_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HGS0772511.1"
                     /translation="MATDKNRIMISLDDKNLEKLENLVEDARDRRGMRLTKSQVIELL
                     LNTVDYFDDIMGAIYSKK"
     gene            3093..3851
                     /locus_tag="ACL73L_001557"
     CDS             3093..3851
                     /locus_tag="ACL73L_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HGS0772512.1"
                     /translation="MVVYIRQSKLPSEVSINKYNAQVGAYLQGEEVILYQSFYEIKEL
                     TSEDIVVDYIMETRALLKMMGLNVPVYDYPIELKEFYGRKIYVGILGEIVNIPDNWGK
                     FIKPKAGSKVFTGRVVNGTHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLDVR
                     PYTGDYHAQFDASVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYGLS
                     PLKSINFHRARWKEMVKPYFEKNEIFKIQQDVIF"
     gene            3909..4763
                     /locus_tag="ACL73L_001558"
     CDS             3909..4763
                     /locus_tag="ACL73L_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HGS0772513.1"
                     /translation="MLGAIIGDIVGSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGRMYPNADYGARFNAWLNSDNREPYNSFGNGSAMRISPC
                     AWIMDCGFYAKTGIWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRFYFGGYCR
                     EYEQSINDNPTECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQETVPQAIIAFL
                     ESRDFEDAIRNAISLGGDSDTLAAITCNIAEAAYGIPDWIKDKAYSYLDEPLKDVVRR
                     WENRIKAY"
     gene            4875..5759
                     /locus_tag="ACL73L_001559"
     CDS             4875..5759
                     /locus_tag="ACL73L_001559"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HGS0772514.1"
                     /translation="MKKAMVIINPTSGGEKALDYKEKLENKAKEYFEYVETKITEKAL
                     DATHFAEEASREQYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLE
                     INQDIDGAIDELDFDLTNKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKFGILT
                     YAVNTMKSVMTDQVFNIKVETENGNYVGEASHVLVLLTNYFADKKTFEENKDGYANIL
                     ILKDASIFSKLSVIPDLLKGDVVANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKPKED"
     gene            5921..6654
                     /locus_tag="ACL73L_001560"
                     /pseudo
     CDS             5921..6654
                     /locus_tag="ACL73L_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511339.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase N-terminal ig-like
                     domain-containing protein"
     gene            6631..7056
                     /locus_tag="ACL73L_001561"
                     /pseudo
     CDS             6631..7056
                     /locus_tag="ACL73L_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500472.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7501..7977
                     /gene="pezA"
                     /locus_tag="ACL73L_001562"
     CDS             7501..7977
                     /gene="pezA"
                     /locus_tag="ACL73L_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PezA"
                     /protein_id="HGS0772515.1"
                     /translation="MIGKNIKSLRKTHDLTQDEFARIVGISRNSLSRYENGTSSVSTE
                     LIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIEIVKERGANLLSRLYRYQDSQGI
                     SIDDESNPWILMSDDLSDLIHTNIYLVETFDEIERYSGYLDGIERMLEISEKRMVA"
     gene            7977..8753
                     /gene="pezT"
                     /locus_tag="ACL73L_001563"
     CDS             7977..8753
                     /gene="pezT"
                     /locus_tag="ACL73L_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin PezT"
                     /protein_id="HGS0772516.1"
                     /translation="MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAG
                     KTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQEYGKDSVEYTKDFAGKMVESLV
                     TKFSSLRYNLLIEGTLRTVDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
                     LYIINPNQARATPKEHHDFIVNHLVDNTRQLEELAIFERIQIYQRDRSSVYDSKENTT
                     SAADVLQELLFGEWSQVEKEMLRVVEERHKELEGKNSYGI"
     gene            8743..10500
                     /locus_tag="ACL73L_001564"
     CDS             8743..10500
                     /locus_tag="ACL73L_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGS0772517.1"
                     /translation="MEFKKLVIENFRNFSSIEVALSNKNVIFGMNDSGKTNLLFAIRY
                     LLDRTIRNKGFIKSDYYRHDTSRPIKIQLELDLSDRKMEGDEESLKSSHSRLLISTVA
                     GARNNSSLDTFFISLEAVYNEKELFGNPVLSWGSTLDNLVEVPQYGTRSDIDKIFQIV
                     YVNPAIELDSFFKRNRRILFSDDSKTDEDVKLEEEIENNIEKLNENISNLHLITSVQS
                     QLTEAYKSYRKENLEIKIQSEISISGYLDNLTPYINWNDDTHNYPTSGDGRKKLLSYA
                     INHLISEKQYNKKVIIYLIEEPENSLHRSVQISLSKQLFVQNIYEYFFLTTHSAEVLY
                     EMDNTQLIKITNQQDATGQSVYYTVPNEFSNLKKKLNKSLAQSIFYDRILLVEGPSEY
                     YLFSAIFEYVSPNFEADGKYLLQVDGINFKPYSELYKALGIDFWIKTDNDLKASRGDK
                     HVFSPIGLNRCIDLLDHSATPILDNYRIDFSSKDERKQNIKSKKEELYLTNHNLVLQL
                     NADGIFISEIDLENDLAQVLSDSERNEMSKLDFVNWLQSSKQYNMLEYISNHLTKEIA
                     LKIVESGYFKVLAEFLDYE"
     gene            10493..>11359
                     /locus_tag="ACL73L_001565"
     CDS             10493..>11359
                     /locus_tag="ACL73L_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HGS0772518.1"
                     /translation="MSDIATQDKNVKKELLEYQGNILILANAGSGKTTFLTKKLKSDS
                     KRLNNYQKLAAITFTRNATEEIKQKLVDIPDNVVVSTIDSFLDKEIILPFLNQKYEIQ
                     TSVQFSFRIDHNFSDYSTGIKQILQDGIFGTYDFPTSKHGKNFKCEVALDILKTIDSA
                     SEYIKYKFNTLYIDEFQDCDQSMNDLFLYLKNELGIPLFIVGDDKQSIYQWRGASPKY
                     INNLWKNKNDFRKKRFIGNFRSLPRIVDFSLALTPEVTVDNIREEGSILYLEPNQDYP
                     KEDIIKYLIDNGD"
BASE COUNT         4075 a         1520 c         2283 g         3481 t
ORIGIN      
        1 taatactatg tcaaaagaaa aaataaaagt atacctctat acacgagtat ctacgtcaat
       61 acagatagag gggtattcgt tagaggcaca aaaatcacga atgaaagctt ttgctattta
      121 caatgattat gaaattgttg gagagtatga agatgccggg aagtctggca agtctattga
      181 aggtagaata cagttcaatc gaatgatgga agatataaaa tctggaaaag atggagtatc
      241 ttttgttctt gtgtttaagt tatcaagatt tgcaagaaat gccgctgatg ttctttctac
      301 acttcagata atgcaggatt atggagttaa tcttatctgc gttgaagatg gcattgattc
      361 atctaaagat gctgggaaac taatgatttc tgttttatca gctgtggctg aaatagaaag
      421 agaaaacatc cgtattcaaa caatggaagg tcgcattcaa aagcaaggga aggaaaatgg
      481 aatggtggct ttgctccgta tggatataaa cttgaagacg gcaagctgtt tataaatgag
      541 gaagaggcag ttgcaataag aacgattttc gaccagtatg taaatactac gataggagcc
      601 aatgggatat ctaaatactt agagaatcat ggaattagaa aaatcccaag acagaatggt
      661 aagaatcctt tgtttgatgc aggtcttata agaaagatat taaagaatcc tgtatataat
      721 gggaaaatag tatttggaag aagaacttta gaaaaagttc atggtacaag aaatgaatat
      781 aagcaggttg aacaagatga atatctaata tctgaaggga tacatgaagc tatagtttcc
      841 gatgaagttt ggcaagctgc tcaggttaag ctaaaatctc aagcaaagaa atatgagcat
      901 gtgaataaag gaaaagatac acgcacacac ttgctttctg gaattgtaaa atgccggata
      961 tgtggagtgg gaatgtttgg gaacaagtgt atcaagaaaa agaaagatgg cacaaagtat
     1021 aaagattttt attactatgg ttgtaaacat aggcagatga taagaggtca taagtgtaca
     1081 ttcagtaagc aaattagaga agaattgtta gatgatgctg ttgcagaggt gattgtcaag
     1141 atagtaagta atccgaaatt tgcttctatg atgcaagaaa aaatcaacat gaaggtggat
     1201 acctctgaaa tagaaaaaga aatagataat taccaaaagg aattgagaaa gagtcattct
     1261 acaaagttta agctaattga ggaaatagat aatttagatg ttgaagataa gcactataag
     1321 agaaggaaac aggatttaga tgatagacta tatcgtatgt atgacaaaat agatgaatta
     1381 gaatcatcac taattgatgc gaaagcaaag aaacagacta ttgaagctga aaagcttaca
     1441 ggagataaca tatataaggt tctgatctat tttgataagc tctataaagt catgaatgat
     1501 gtagagcgta ggcagttaat ttcagctttg atttctgaaa ttcaagttta tgaagaaaaa
     1561 caatctaacg gacaatggct aaaatccatt acttttaagc taccaattat agaagaaaat
     1621 ctcaacatag gtttggacaa tgatgaacaa gttgaatgtg taagtttatt agagaaacgt
     1681 agttaatcct caaaaggttc cccgaacctt ttgagtccca ctgacgcatc acgctgaggc
     1741 ggtatcactg cttgtacgag ttgaagcatc agcgaagtaa aagtagatgt tcagccaatg
     1801 caacgaggtg agaccgaagg tcgataaggt gtgttctggg tataggactc gtaaagtaag
     1861 taggaaatat gatgagatga tacagagagc ctgaattggt caggtgacgt gcttatttac
     1921 attagcctga taatgaaaat ggagagatta tcgatggtta tattggaatt gtaccaaaat
     1981 gattattcta aagatttagt agcatttgat tccatagaag atgggaaagc ctttgtagct
     2041 caaatcccgg gatacactct ggaaacagaa gatggttttg aagtggagta ttttaatcct
     2101 aaaaacatac cggattatat ggaaattatt tttaacggaa atattgttcc tttatctaaa
     2161 tttatgttcg atcctgggga aaatgtaaac attatttgga aagagatttc aaatttatcc
     2221 ctcaaaaatg atagagtgat cgaaggatat tcaaaaattg atgcatatgt cgttaacaac
     2281 catgaagtta aagcctatgt cgaaacaaga gaaacgaact atcgtaaagc aaaagacttc
     2341 ttagaaagcc gcggatatga aatcgacaga agctttttcg gaagtgaaga cggtgaagcg
     2401 attctttaca gaaaaaaagg gatcgaagtt tggcatttct tgtgtcactt agacccaatg
     2461 tttgtagaga ttgaagatgt agaaggatat gttaaagaag aagttggaga gattcaatag
     2521 tcaaccaaag ttccttgtga gtatatacct ttgctagtaa aagagtcaaa acgttataac
     2581 gaaggggggg gaagaaaatc cttgagtgta agtctttttc ttgtttagag ggaaagtatt
     2641 agagtagccg ctcataattt atactagatt tgataactga tttgagacag attttatttc
     2701 tataaatatt gtgaaaattt tttaatacca aagatttaaa gttactgtgt aaaaaatcct
     2761 ctccactttt aaactaatgt attataaaag ccagttaaca caagtgttac acctgtgata
     2821 gaattaactt gaggtgatga aaatggcaac cgataaaaat agaataatga ttagcttgga
     2881 tgataaaaat ctagagaaac ttgaaaattt agtagaagat gctagagata gaaggggaat
     2941 gcgactaaca aagtcccagg ttattgaatt actcctaaat accgttgatt atttcgatga
     3001 tattatgggg gctatttact caaaaaaata agagcaaaat tgctattatt ttttacaaat
     3061 tggtgtaaca ctagtgatac aggagtgatg aaatggttgt ttatattaga caaagtaaat
     3121 taccaagtga ggtttcaatt aataaataca atgctcaagt aggtgcctac ttacaaggag
     3181 aagaagttat tttatatcaa tctttttatg aaataaaaga gttaacaagt gaagatatag
     3241 ttgtagatta tataatggag actagagcat tactaaaaat gatgggctta aacgttccgg
     3301 tttatgacta tcctattgag cttaaagagt tttatggtcg aaagatttat gttggtattt
     3361 taggagagat tgtgaatata cctgataatt ggggaaaatt tattaagcct aaagctggtt
     3421 caaaagtctt tactggaaga gttgttaatg gaacccatga tttaataggt attggtctac
     3481 ctttcgacta tcctatatgg attagtgagg ttgtagaatt catagctgaa tggcgttgtt
     3541 ttgtgttaga tggtcgcgta ttagatgttc gaccctatac aggtgattat catgcacaat
     3601 ttgatgcaag tgtaattgat gaagcgatat catgttggaa agatgcgcca atagcttatg
     3661 gactagatat cggtgttact cgcgatggca gaacacttgt tgttgaagta aatgatggtt
     3721 atgcattggg aaattatggc ctatctcctt taaaatcaat caattttcat agagctaggt
     3781 ggaaagaaat ggtaaaaccc tattttgaaa aaaatgaaat ttttaaaatc caacaggatg
     3841 ttattttcta aaataaatat ggtagataac caatataaaa agcatcaaaa tgaacaagga
     3901 ggaatctgat gcttggagca attattggag atattgtcgg ttctgtttac gaatggaaca
     3961 atatcaaaac aaaggacttt cctttatttc ggaaggactg cttttttaca gatgacacgg
     4021 ttatgacttg tgctgttgca gaagcaatta tgaatggtgg acaaaaagac gacttcattg
     4081 acgcgatgaa gaaatatggc agaatgtatc cgaatgctga ttacggtgct cggtttaatg
     4141 catggctaaa cagcgataac cgtgagcctt ataatagctt tggtaatgga tcagctatgc
     4201 gtatttctcc atgtgcttgg atcatggact gtggttttta tgcgaaaact ggtatatggc
     4261 catcatctag aggacttacg agtctttctg cagaggtaac tcataatcat ccagaaggtg
     4321 tcaaaggcgc tatggctaca gctgatgcta tctttctatg tcgtttttac tttggtggtt
     4381 attgtagaga gtacgagcaa tcaattaacg acaatcctac agagtgtaaa agacgaatta
     4441 aggattatat agaaaaagaa tacgattaca atctatctca aactttagat gaaatccgtc
     4501 ctaactatcg ttttaacgaa acatgtcagg aaactgtccc tcaagccatt atcgcctttc
     4561 ttgaaagtag agacttcgaa gatgcgataa gaaatgccat ctcacttggt ggcgacagtg
     4621 atactctcgc agcaatcact tgtaacatag cagaggcagc ttacggtatt cctgattgga
     4681 tcaaggataa ggcctattct tacttggatg aacccttaaa ggatgtagtt aggcgatggg
     4741 aaaatagaat aaaagcgtat taatctccag tacgtcagat ttctcttttc tgtattttca
     4801 ttgtataata ggattacatt tacttgcata cgacgaaatg taaacatttc tgtttaaaat
     4861 taaggataaa atcaatgaaa aaagcaatgg taattatcaa ccctacttct ggtggcgaga
     4921 aggctttgga ttacaaagaa aagctggaga ataaagcaaa agaatacttt gaatatgttg
     4981 aaaccaaaat taccgaaaaa gcgctggatg caacacattt tgctgaagaa gcttctcgtg
     5041 agcagtatga tgcagtggtt gtgtttggtg gagatggaac tgtcaatgaa gtcatttcag
     5101 gtattgatga gagagactac attcctaagt tagggattat cccaggcggt acgggtaacc
     5161 tcattacaaa acttttggaa atcaatcaag acatcgatgg cgcaattgac gaactggatt
     5221 ttgatttaac caataagatt gatatcggta aagcaaatga caactatttt ggttatatct
     5281 ttagtatcgg ttctctgcct gaggcgattc acaatgttga aatagaggac aaaacaaaat
     5341 tcggtatttt aacctatgct gtaaatacca tgaagtctgt catgacagat caggtcttta
     5401 atattaaggt tgagacagaa aatggaaatt atgttggtga agctagccat gttttggtcc
     5461 ttttgacaaa ttacttcgct gataagaaaa cctttgaaga aaacaaggac ggctatgcca
     5521 acattttgat tctgaaagat gcctctatat tctccaaatt atccgtcatt cctgatttat
     5581 taaaagggga tgttgtcgca aatgataata tcgagtatat caaagcgcgt aatattaaaa
     5641 tctcttcaga tagtgaattg gagtcagatg ttgacggaga taaatcagat aacctacctg
     5701 tagaaatcaa agtcctagct cagcgagtag aagtattttc aaaaccgaaa gaggattagt
     5761 atatagagaa agccttttta aggctttttg tatactttaa aagatagttc ctttaacaac
     5821 ggacattcct tgtaaatagt ttacaaaaat agtatactgg atttattgag tttgaaaacg
     5881 tttgcgtaaa atttgaatga atactttagg agacaaattg atggaattga gtgctattta
     5941 ccataggcct gagtcggagt atgcctatct ttataaggat aagaaactcc atattcgaat
     6001 tcgaactaag aaaggggaca ttgaaagcat taacttgcac tatggggacc cttttatctt
     6061 tatggaggag ttttatcagg atacaaaaga aatggtcaag ataacttctg gtaccttatt
     6121 tgaccattgg caggttgaag tgtcagttga ctttgcacgt atccagtatc tctttgagct
     6181 cagagataca gaaggtcaaa atattttgta tggcgataaa gggtgtgtgg aaaattctct
     6241 agaaaatctt catgcaattg ggaatggatt taagttgcct tatcttcatg agattgatgc
     6301 ctgcaaggtt cctgactggg tttcagatac ggtatggtat cagatatttc ctgaaagatt
     6361 tgccaatggc aatgctctat taaacccaga agggacttta gactgggatt catctgtcac
     6421 acctaagagc gatgatttct ttggtggtga tttacagggg attattgatc atatggatta
     6481 cttgcaagac ttgggtatta ctggactata tctttgtccc atctttgaat ctacaagcaa
     6541 tcacaagtac aatacgacag attactttga aattgaccgt cattttggag acagtgttgc
     6601 atgggtgcgg caagggattt tttagaacta attaatcaca aagggctacc tctttttaat
     6661 ccgcttgtag gtgattctca tgtaaataat agacaagaat atgacaatac agggagtgga
     6721 aatttacaac ctgaaaagtg gaatgaaact gcaaagtagc tttataatgc tataatgtgg
     6781 ttgattattt tatggaatgc taagccggat acacctttat ttaattttaa agacgaagta
     6841 attaagtata aaacatatga gccttttgaa agcagtataa aaagagtaaa tactactata
     6901 aagaatggta gtaaagggaa aactctgact gagatgatta atggctacag agcggataac
     6961 gatattagag atgaaatttg taactttaat attctgaaaa ataaaattcg tgatatgaaa
     7021 gaccaacaag gaaatacaat ggaatcttac ttttagttat tgttgaattt tgggtattct
     7081 ataaaatatc ctaattgaga tttaaatagt agactataca atatagttaa aatatcagta
     7141 aaaacaacac tttattgagg tattggatac gctttgctaa tagcctaata atcacatgtg
     7201 gagtgttgct acaacgaaaa aggtgataat ccttgatttc aagctatttt ataagcattt
     7261 tgtctttgta gataaaggca attttgacaa taaaaatcct aaaaggtgaa tcgttataga
     7321 tgtatttgta gatatcgttt gcgcatcgaa aaaattaata caagaataaa tatttatggc
     7381 tctttaggtg acttttatag aagtaaagtt taggatagaa aaacaagaaa taatgcacca
     7441 tttttggtgc attatgcttt tttatgctat aattgaatta taaaaataaa ggagtttgcc
     7501 atgattggaa agaacataaa atccttacgt aaaacacatg acttaacaca agacgaattt
     7561 gcacggattg taggtatttc acgaaatagt ctgagtcgtt atgaaaatgg aacgagttca
     7621 gtctctaccg aattaataga catcatttgt cagaagttta atgtatctta tgtcgatatc
     7681 gtaggagaag ataaaatgct caatcctgtt gaagattatg aattgacttt aaaaattgaa
     7741 attgtgaaag aaagaggtgc taatctatta tctcgactct atcgctatca agatagtcag
     7801 ggaattagca ttgatgatga atctaatcct tggattttaa tgagtgatga tctttctgat
     7861 ttgattcata cgaatatcta tttagtagaa acttttgatg aaatagagag atatagtggc
     7921 tatttggatg gaattgaacg tatgttagag atatctgaaa agcggatggt agcttaatgg
     7981 aaatccaaga ttatactgat agtgaattca aacatgcttt agcaaggaat cttcgttcac
     8041 tgacaagagg aaaaaagtcc agtaagcaac ctatagcgat tttgcttgga ggtcaaagtg
     8101 gtgccggtaa gactacaatt catcgtatta aacagaaaga atttcaagga aatattgtta
     8161 tcatagatgg tgatagtttt cgttctcagc atccacacta tttagaactg cagcaagaat
     8221 atggcaaaga cagtgtagaa tataccaaag attttgcagg aaaaatggta gagtctttag
     8281 taacaaaatt tagtagtttg agatacaatc ttttgataga gggaacttta cgaacagttg
     8341 atgttccaaa gaaaacagca caactcttga aaaataaggg atatgaagta caattggcct
     8401 taattgcgac aaagcctgaa ttgtcgtatc taagtactct tatccgttat gaagaactgt
     8461 acattatcaa tccaaatcaa gcacgcgcaa ctccaaaaga acatcatgat ttcattgtaa
     8521 atcatctagt tgataataca cgacaattgg aagaactggc tatctttgaa agaattcaaa
     8581 tttaccaacg agatagaagt tctgtatatg attcaaaaga aaatacaact tcagcagcag
     8641 atgttcttca agagttactc tttggggagt ggagtcaggt agagaaagag atgttgaggg
     8701 tggtggaaga aagacataaa gagttagaag gaaaaaatag ttatggaatt taaaaaacta
     8761 gtaattgaaa attttcgtaa ttttagttct attgaggttg ctttatcaaa taaaaatgtt
     8821 atatttggaa tgaatgattc agggaaaaca aaccttctat ttgcaataag atacctcttg
     8881 gacagaacta tacgaaataa aggttttatt aaatcggatt attatagaca tgatactagt
     8941 agacctataa aaatacaact tgagcttgat ttgtctgata gaaaaatgga gggggatgaa
     9001 gaatcactaa aatcttcaca ttctcgtctt ctaatttcaa cggtagctgg agcaagaaat
     9061 aactctagct tagatacatt ctttatctca ttagaagcag tttataatga gaaagagtta
     9121 tttggaaatc cggtattaag ctggggctca acactagata atttagtgga agttcctcaa
     9181 tatggtacaa gaagtgacat tgataaaata ttccaaatag tttatgtaaa tcccgctata
     9241 gagttagatt ccttttttaa gcgaaataga aggatattgt tctctgatga ttctaaaact
     9301 gatgaggatg taaagcttga ggaagagatt gagaataaca ttgaaaaatt aaatgaaaat
     9361 atttctaact tacatttgat tactagtgtt caatcacagt tgactgaagc ctataaaagt
     9421 tatcgtaaag aaaatttaga aattaaaatt caatcagaga tatctatttc aggatattta
     9481 gacaatctca caccatacat taactggaat gatgatactc acaattatcc gacgtcagga
     9541 gacggtcgta aaaagctttt atcatatgct attaaccatt taatttcaga gaaacaatac
     9601 aacaagaagg taattattta tttaattgag gagccagaga atagcttaca tcgttccgtt
     9661 caaatctctc tttcaaagca actttttgtt cagaatattt atgaatactt ttttctaacg
     9721 actcattcag cagaagttct ttatgaaatg gataatactc agctcattaa gataaccaat
     9781 caacaagatg caacagggca atcagtctat tacacagttc ctaatgaatt ttcaaactta
     9841 aagaaaaaat taaataaatc gttggctcag tcaatctttt atgataggat tcttttggtc
     9901 gaaggtccat cagaatatta tctattttct gctatttttg aatatgtaag tcccaatttc
     9961 gaggctgatg gaaaatactt attacaagtt gatggaataa attttaaacc ttattcagag
    10021 ctatataagg cattagggat tgatttttgg attaaaactg ataatgattt aaaagctagt
    10081 cgtggagata aacatgtatt ttctcctatt ggcttaaata ggtgtattga cttgcttgat
    10141 catagtgcga ctcctatatt agataattat cgtatagatt tctcttcaaa agatgagaga
    10201 aaacagaata taaagtctaa aaaagaagaa ttatatttga ctaatcataa tctagtatta
    10261 caattaaatg cagatggaat ctttatatcg gaaattgatt tagaaaatga tttagctcag
    10321 gttttatccg atagcgagag aaatgaaatg agtaaattag attttgtgaa ttggttacaa
    10381 agcagtaaac aatacaatat gctagaatat ataagtaatc atcttactaa agagattgcg
    10441 cttaagattg tggaatcagg atattttaaa gttttggcgg agtttttaga ctatgagtga
    10501 tattgctaca caggacaaga atgtcaaaaa agaattgtta gaataccaag gaaatatttt
    10561 aattttagcg aacgcaggtt cgggaaaaac aactttttta actaaaaagc taaaaagtga
    10621 ttctaaacga ttaaataatt atcaaaaatt agcagcaata acttttactc gaaatgcaac
    10681 ggaggaaata aagcaaaaat tagttgatat tccggacaac gttgttgtat cgaccataga
    10741 ttcgttttta gataaagaaa taatccttcc ttttcttaat cagaaatacg aaattcaaac
    10801 atctgtacag tttagctttc ggatagacca taattttagc gactatagca ctggaataaa
    10861 acaaatattg caggatggta ttttcgggac ttacgacttt cctacttcta aacacggcaa
    10921 gaatttcaaa tgtgaagttg ctttagatat tttgaagaca atagattctg cgtcagaata
    10981 tataaaatat aaatttaata ctttgtatat tgatgaattt caagattgtg accagtctat
    11041 gaatgattta tttctgtatc taaaaaatga gttagggatt cctttattta ttgtaggaga
    11101 tgataagcaa tctatttatc agtggagagg agcatcaccc aaatatataa ataatttatg
    11161 gaaaaacaaa aatgatttta gaaaaaagag atttattggg aattttagaa gtttgcctag
    11221 aatagtggat ttctctttag ctctgactcc tgaagtgact gtcgataata ttagagaaga
    11281 gggttcaatt ctatatttag aacctaatca ggattatcca aaagaagata ttattaagta
    11341 ccttattgat aatggtgat
//
LOCUS       DBERDK010000057        11137 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.057, whole genome shotgun sequence.
ACCESSION   DBERDK010000057 DBERDK010000000
VERSION     DBERDK010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 11137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11137
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.057"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            98..2320
                     /gene="recJ"
                     /locus_tag="ACL73L_001566"
     CDS             98..2320
                     /gene="recJ"
                     /locus_tag="ACL73L_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HGS0772519.1"
                     /translation="MITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQNQ
                     ESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEEGENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSMPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEET
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPHRDLFIAFGGHAGAAGMTLEVEQLSDLSQVLEDYVREKGADAGGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GEASFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMVDARVEGVQLFN
                     IRGKNAVLPEGVPVLDFPGELPNLAASEAVVVKNIPEDITQLKTIFQEQHFSAVYFKN
                     DIDKAYYLTGYGTRDQFAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE
                     "
     gene            2495..4156
                     /locus_tag="ACL73L_001567"
     CDS             2495..4156
                     /locus_tag="ACL73L_001567"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HGS0772520.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQIFDAADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEIL
                     LIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMAVGMLPERIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNAIGDVGNV
                     VLRDRKVLSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILRESSELINQT
                     VEEYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            4225..5004
                     /locus_tag="ACL73L_001568"
     CDS             4225..5004
                     /locus_tag="ACL73L_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGS0772521.1"
                     /translation="MAVMKIEYYSQVLDMEWGVNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRGTNLIVVMPNTSNGWYTDTQYGFDYYTALAEELPQVLK
                     RFFPNMTSKREKTFIAGLSMGGYGCFKLALTTNRFSHAASFSGALSFQNFSPESQNLG
                     SPAYWRGVFGEIRDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLAVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT"
     gene            5116..6336
                     /gene="murM"
                     /locus_tag="ACL73L_001569"
     CDS             5116..6336
                     /gene="murM"
                     /locus_tag="ACL73L_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HGS0772522.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFAIQSIKSYARSKRAV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQAIRTARNKGLEIQYGGLELLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFKDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRTSKVEAQKKE
                     KERLLEELTFLQEYIDVGQARVPLAATLSLEFGTTSVNIYAGMDDDFKRYNAPILTWY
                     ETARYAFERGMIWQNLGGVENSLNGGLYHFKEKFNPTIEEYLGEFTMPTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            6341..7573
                     /gene="murN"
                     /locus_tag="ACL73L_001570"
     CDS             6341..7573
                     /gene="murN"
                     /locus_tag="ACL73L_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HGS0772523.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     VKPYETYQTFDSQGNPIDAEKKSIIQDLTDLGYQFDGLTTGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKETSERREYSDKSLEY
                     YEHFYDTFGEQAEFLIASLNFSEYMSKLQGEQSKLEEKLDKLRLDLSKNPHSEKKQNQ
                     LREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYLFSGSYTEFNKF
                     YAPALLQKYVMLESIKRGIPKYNFLGIQGIFDGSDGVLRFKQNFNGYIVRKAGTFRYH
                     PSPLKYKAIQLLKKIVGR"
     gene            7652..8353
                     /locus_tag="ACL73L_001571"
     CDS             7652..8353
                     /locus_tag="ACL73L_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M57 family metalloprotease"
                     /protein_id="HGS0772524.1"
                     /translation="MRWLFRLIGAFFSFVWRLFWRLVWIVVLLCVLAFGLLWYLNGDF
                     QGALKQAERSVKIGQQSIDQWEKTGQLPKLSQTDSHQHSEGRWAQASARIYLDPQMDS
                     RFQEAYLEAIQNWNQTGAFNFELVTESSKADITATEMNDGGTPVAGEAESQTNLLTGQ
                     FLSVTVRLNHYYLSNPYYGYSYERLVHTAEHELGHAIGLDHTDEKSVMQPAGSFYGIQ
                     EEDVANLRKIYETSE"
     gene            8393..10237
                     /gene="uvrC"
                     /locus_tag="ACL73L_001572"
     CDS             8393..10237
                     /gene="uvrC"
                     /locus_tag="ACL73L_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HGS0772525.1"
                     /translation="MNNLIKSKLELLPTSPGCYIHKDKNGTIIYVGKAKNLRNRVRSY
                     FRGSHDTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPFI
                     KITNERYPRLIITRQVKKDGGLYFGPYPDVGAANEIKRLLDRIFPFRKCTNPPSKVCF
                     YYHIGQCMAHTICKKDETYFKSMAQEVSDFLKGQDNKIIDELKGKMAAAAQTMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVLNEVLIPQDIDEEAVKALVDTKILKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVVFVNGKPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQREALTPPDLI
                     VIDGGQGQVNIAKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPLEVVDLSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMKHFKSLTKIKEA
                     SVDEIVEVGVPRVVAEAVQRKLNPQGEALPQVAEERVDYQTEGNHNEP"
     gene            10227..11060
                     /locus_tag="ACL73L_001573"
     CDS             10227..11060
                     /locus_tag="ACL73L_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HGS0772526.1"
                     /translation="MNHKIAILSDVHGNATALEAVIADAKNQGASEYWLLGDIFLPGP
                     GANDLVALLKDLPITASVRGNWDDRVLEALDGQYGLEDPQEVQLLRMTQFLMERMDPA
                     TTVWLRSLPLLEKKEIAGLRFSISHNLPNKNYGGDLLVENDTEKFDQLLDAETDVAVY
                     GHVHKQLLRYGSQGQQIINPGSIGMPYFNWEALKNHRAQYAVIEVEDGELVNIQFRKV
                     AYDYEAELELAKSKGLPFIEMYEELRRDDNYQGHNLELLASLIEKHGYVEDVKNFFDF
                     L"
BASE COUNT         3337 a         1958 c         2672 g         3170 t
ORIGIN      
        1 tactataaaa tgttataaaa aagcaatctg gatagagaaa acgtctaaat catgttataa
       61 tgaagcaata gaattcttag aaagagtgga tgtctttttg ataacaccta cttatgaatg
      121 gcagtttgcc ctgcaggtag aagatgcgga ttttacaaag atagccaaga aggctggact
      181 gggtcctgag gtggctcggt tattgtttga gagagggatt cagaaccaag aaagtctgaa
      241 gaagttttta gaaccttcct tggaggactt acatgatgct tatctgctcc atgatatgga
      301 caaggcagtg gagcggattc gtcaggctat tgaagaaggg gaaaatattc ttgtttatgg
      361 agactatgat gcggatggca tgacttcggc ttctattgtg aaggaaagtt tggaacaact
      421 tggtgctgag tgccgagttt acctgccaaa tcgttttacc gatggctatg gccctaatgc
      481 tagtgtttat aaatacttta tcgagcaaga agggatttcc ttgattgtga cggtggacaa
      541 tggggttgct ggtcatgagg ctattgcatt ggctcagtct atgggagtag atgtcattgt
      601 gacagaccat cattccatgc ctgaaaccct gccagatgct tatgctattg tccatcctga
      661 acatccagat gcggattatc cttttaaata tttggctggt tgtggagttg ctttcaagtt
      721 ggcttgtgcc ctgttagaag aagtgcaagt ggaattgctt gatttggtcg ctattggaac
      781 tattgcagat atggtgagtc tgacggatga aaatcgtatc ttagttcaat atggtctgga
      841 aatgttgggt catacccagc gcattggtct gcaagaaatg ctggacatgg ctgggattgc
      901 tgccaacgaa gtaacagaag aaacggttgg tttccagatt gctcctcgtt tgaatgcctt
      961 gggtcgcttg gatgatccca atcctgccat tgatttgttg actggatttg atgatgagga
     1021 agcgcatgag attgccctta tgattcacca gaaaaacgaa gagcgcaagg aaatcgttca
     1081 gtctatctat gaagaagcca agaccatggt ggatcctgag aagaaggttc aggtcttggc
     1141 caaggaaggc tggaatcctg gggttctagg aatcgtggct ggtcgtttat tggaagaatt
     1201 gggacagaca gtcattgttc ttaatataga agacggtcgt gccaagggca gtgctcgtag
     1261 tgtggaagcg gtcgatattt ttgaagctct ggatccccat cgagacctct tcatcgcctt
     1321 tggaggtcat gcaggtgcag cgggtatgac gctggaagtt gagcaactct cagatttatc
     1381 tcaggttttg gaagattatg ttcgtgaaaa aggtgcagat gctggtggca agaataagtt
     1441 aaacctagat gaagagttgg atttggaggc acttagcttg gaaacggtca aaagttttga
     1501 acgtttagct ccttttggaa tggataatca gaaacctatt ttttatatca agaattttca
     1561 ggtcgaaagt gctcgtacta tgggggcagg taatgcccat ctaaagctga aaatttccaa
     1621 gggtgaggcg agttttgaag tggtagcctt tggtcaaggc agatgggcga cagagttttc
     1681 tcaaaccaag aatctagagt tagcggttaa attgtctgtc aaccaatgga atggccaaac
     1741 tgccctccag ttgatgatgg tggatgcgcg agtggaaggt gttcaacttt ttaacattcg
     1801 tggaaaaaat gcagtcttgc cagaaggtgt tccagtcttg gattttcctg gagaactgcc
     1861 aaatcttgcg gctagtgaag ctgttgtcgt aaaaaacatt ccagaggata ttactcagct
     1921 gaagaccatt tttcaggaac agcatttctc tgctgtctat ttcaaaaatg atattgacaa
     1981 ggcttattat ctgacaggtt atgggactag agatcagttt gccaaattgt acaagactat
     2041 ttaccagttc ccagagtttg atattcgcta caagctgaaa gatttggctg catatcttaa
     2101 tattcaacaa atcttgctgg tcaagatgat tcaagtattt gaagaactag gctttgtgac
     2161 gataaaagat ggtgtgatga cagtcaataa agaggcgcca aagcgggaga taggagaaag
     2221 tcaaatttac caaaatctca aacaaaccgt taaagaccaa gaaatgatgg cgctgggtac
     2281 ggtgcaagaa atttatgatt ttttgatgga aaaagagtag aagttaggaa agagttggga
     2341 aatcaactct tttttgaaaa cagaccttca ttttgaaaat catcaaaaaa atggtataat
     2401 ggtaggaaaa gattcggctg aaagtatcag aacttttaga ataagagggt agaattgccc
     2461 tataatcaag ataaactaag attttggagg aaaaatgagt aatatcagtt taacaacact
     2521 tggtggtgtg cgtgagaatg gaaaaaatat gtacattgct gaaattggag agtccatttt
     2581 tgttttgaat gtagggttaa aatatcctga aaatgaacaa ttaggggtcg atgtggtgat
     2641 tccaaacatg gattaccttt ttgaaaatag cgaccgtatt gctggggttt tcttgaccca
     2701 cgggcatgcg gatgccattg gtgctctacc gtatctcttg gcagaggcta aagttcctgt
     2761 atttgggtct gagttgacca ttgagttggc aaagctcttt gtcaaaggaa atgatgccgt
     2821 taagaaattt aatgatttcc atgtcattga tgagaatacg gagattgatt ttggtgggac
     2881 agtggtttcc ttcttcccta cgacttactc cgttccagag agtctgggaa ttgtcttgaa
     2941 gacatcggaa ggaagcatcg tttatacagg tgacttcaaa tttgaccaaa cggctagtga
     3001 atcttatgca actgattttg ctcgtttggc agagattggt cgtgacggcg tcctggctct
     3061 cctcagtgat tcggccaatg cagacagcaa tattcaggtg gctagtgaaa gtgaagttag
     3121 ggatgaaatt acccaaacta ttgctgactg ggaaggtcgt atcatcgttg cagctgtttc
     3181 cagtaatctt tctcgtattc agcagatttt tgacgctgcg gataaaacag gtcgacgtat
     3241 cgtcttgaca ggatttgata ttgaaaatat cgtccgcaca gcgattcgtc ttaagaagtt
     3301 gtctttagcc aacgaaattc ttttgattaa gcctaaagat atgtctcgct ttgaagacca
     3361 tgagttgatt attcttgaga caggtcgtat gggtgagcct atcaatggac ttcgtaagat
     3421 gtcgattggt cgccatcgtt atgtagaaat caaggatggg gacctagtct atattgctac
     3481 ggctccgtct attgctaaag aagcctttgt tgcgcgtgtg gaaaatatga tttatcaggc
     3541 aggtggggtt gtcaaattga ttacccaaag tttacatgta tcagggcacg gaaatgtgcg
     3601 tgatttgcag ctgatgatca atcttttgca acctaagtac ctcttccctg tccaagggga
     3661 gtatcgtgag ttggatgctc acgctaaggc tgccatggca gttgggatgt tgccagaacg
     3721 catcttcatt cctaaaaagg ggacgaccat ggcttacgag aatggagact ttgttccagc
     3781 tggatcggtt tcagcaggag atatcttgat tgatgggaat gccattggtg atgttggaaa
     3841 tgttgttctt cgtgaccgta aggtcttgtc agaggatgga attttcatcg tggctattac
     3901 agtcaaccgt cgtgagaaga aaattgtggc tagggctcgt gttcacacgc gtggatttgt
     3961 ttatctcaag aagagtcgcg atattctccg tgaaagttca gaattgatta accaaacggt
     4021 agaagagtat cttcaaggag atgactttga ctgggcagat ctcaaaggta aggttcgtga
     4081 caatctgacc aagtacctct ttgatcaaac caagcgtcgc ccagccattt taccagtagt
     4141 catggaagca aaataatcgt tgaaataaac agagagaaag tcgagtttcg gctttttctt
     4201 atagaaaaat agaaggagaa aatcatggca gtaatgaaaa tcgagtatta ctcacaagta
     4261 ttggatatgg agtggggggt gaatgtcctc taccctgatg ccaatcgagt ggaagaacca
     4321 gagtgtgaag atattcccgt cttgtacctt ttgcacggga tgtctggaaa tcataatagt
     4381 tggcttaagc ggaccaatgt agaacgcttg cttcgaggaa ctaatctcat cgttgttatg
     4441 cccaatacca gcaatggttg gtacaccgat acccagtatg gttttgacta ctacacggct
     4501 ctagcagagg aattgccaca ggttctgaaa cgcttcttcc ctaatatgac gagcaagcgt
     4561 gaaaagacct ttatcgctgg tctttctatg ggaggctacg gctgcttcaa actggctctt
     4621 acgacaaatc gtttttctca tgcagctagt ttttcaggtg ccctcagctt tcaaaacttt
     4681 tctcctgaaa gtcaaaatct gggaagtcca gcctactgga gaggtgtttt tggagagatt
     4741 agagactgga caactagtcc ctattctctt gaaagtctgg ctaaaaaatc ggataaaaag
     4801 accaaacttt gggcgtggtg tggcgaacag gatttcttgt acgaagccaa taatctcgca
     4861 gtgaaaaatc tcaaaaaact aggttttgat gtgacctata gccatagcgc tggaactcac
     4921 gagtggtact actgggaaaa acaattggaa gtttttttaa caaccctacc aattgatttc
     4981 aaattagaag agagactgac ttagtttgaa cttcagcata gggggagtag aactaaaata
     5041 aaatatgttt tcactagact tttcaaacga aagtagtaga atagtaataa aatactggag
     5101 gaaagagagt aggaaatgta ccgttatcaa attggcattc ccacattaga atatgatcag
     5161 tttgtcaaag aacatgaatt agccaatgta ttacaaagta gtgcttggga ggaagttaag
     5221 tctaattggc aacatgagaa gtttggtgtt tacagggaag aaaaattact ggcgacagct
     5281 agtattttga ttagaactct tccgctaggc tataaaatgt tttacatccc aagaggacct
     5341 atattggatt atggggataa agaactcttg aattttgcca ttcagtctat taagtcctat
     5401 gctcgcagta agagagcggt ttttgtgact tttgacccaa gtatttgcct atctcaaagt
     5461 ttaatcaatc aggaaaagac agaatttcct gaaaatctgg ctattattga tagtttgcaa
     5521 caaatgggag taaggtggtc aggaaaaacg gaggaaatgg gagacaccat tcaacctcgt
     5581 attcaggcga aaatatacaa ggaaaatttt gaagaagata aactttccaa gtcaacaaaa
     5641 caggctattc gaacagcacg aaacaaaggg cttgagattc aatatggtgg actggaacta
     5701 ttagattcat tttcggagtt gatgaaaaaa actgagaagc gaaaagagat tcatttgagg
     5761 aatgaagcct attataaaaa attgttagat aattttaagg acaaggccta tatcaccttg
     5821 gccaccttgg atgtttctaa acgttcgcaa gagttagaag aacagttagc gaaaaataga
     5881 gccttggaag agacctttac tgagtcgact cgaacttcaa aagtagaagc gcagaagaag
     5941 gaaaaagaac gtttgttaga ggaattgacc ttcttgcagg aatatataga tgtaggtcaa
     6001 gcgagagttc ctttagcggc tactttgagt ttggaatttg gtactacctc tgtcaatata
     6061 tatgctggta tggatgatga ttttaaacgt tacaatgcac caattttaac ttggtatgaa
     6121 acggctcgct atgcctttga acgaggtatg atctggcaaa atttaggtgg tgttgaaaac
     6181 tctctcaatg gtggacttta tcattttaag gaaaaattta atccaacgat tgaagaatac
     6241 ttgggtgaat ttacaatgcc cactcatcct ctctatcctc tgttaagact tgctcttgat
     6301 ttccgtaaaa cattaagaaa aaaacataga aagtaagtat atggcactaa caacactcac
     6361 gaaagaagag tttcagactt attctgatca ggtttcttct cgttccttta tgcaatctgt
     6421 ccagatgggg gatttgctag aaaaaagagg ggctcgaatt gtttatcttg ctttgaaaca
     6481 agaaggagaa attcaagttg cagctctggt ttatagcctg cccatgctgg gtggtctgca
     6541 tatggaactc aattcggggc cgatttatac ccaacaagat gctcttccag ttttttatgc
     6601 agagttaaaa gaatatgcca agcaaaatgg tgtattagag ttgcttgtaa aaccctatga
     6661 aacttatcaa acttttgata gccaaggtaa tccaatagat gctgagaaaa aaagtattat
     6721 tcaagatttg actgatttag gttatcaatt tgatggctta acaacaggtt acccaggtgg
     6781 agaaccagat tggttatact ataaagattt aactgaatta actgaaaaga gtttgcttaa
     6841 aagttttagc aaaaagggta aacccttggt gaaaaaggct gaaacctttg gcattcggtt
     6901 gaaaaagtta aaacgtgaag aactatcgat ttttaagaat ataacaaaag aaacctctga
     6961 acgtagagaa tatagtgata aaagtttaga atattatgag catttttatg atacttttgg
     7021 agaacaagcg gagtttctca tagcaagctt gaatttttcg gagtatatga gcaaattgca
     7081 aggtgaacaa agtaaactag aagaaaaatt ggacaagttg cgacttgatt tgagtaaaaa
     7141 tcctcattct gagaaaaaac aaaatcaact gagagaatat tctagtcaat ttgaaacgtt
     7201 tgaagttcga aaagcagaag cgcgagactt gattgaaaaa tatggagaag aagatattgt
     7261 tttagctggt agtttatttg tttatatgcc tcaggaaacg acttatctct ttagtggttc
     7321 ctacactgag tttaataagt tctatgcccc tgcactgctt caaaaatatg ttatgttgga
     7381 aagcataaaa cgtggaatac ctaaatacaa cttcctaggc attcaaggga tttttgatgg
     7441 aagtgatggt gttttgcgtt ttaaacagaa ttttaatggc tatattgtac gcaaagcggg
     7501 tactttccgt taccatccat cgcctttaaa atacaaagct atccagttac tcaaaaaaat
     7561 agtaggacgt taagatgaaa aagtcagtat ttagatttct tttagcttct tttagtaaaa
     7621 taattcttat ttgctagaaa ggtggagaga catgcgctgg ctttttcgtt tgataggggc
     7681 tttcttttct tttgtgtggc gtttgttttg gcgtctggtt tggatagttg tgctcttatg
     7741 tgtgcttgct ttcggacttc tctggtatct gaacggggat tttcaaggag cgctaaagca
     7801 agcagaacgg tcagtaaaaa ttggtcaaca aagtattgac caatgggaga aaacagggca
     7861 actgcctaag ttaagccaga cagatagtca ccagcattct gaaggaaggt gggcacaggc
     7921 ctctgctcgt atttacctgg atccgcagat ggattcacgc tttcaagagg cttatttaga
     7981 agcaatccag aactggaatc aaacgggtgc ttttaacttt gaactcgtga ctgagtctag
     8041 taaggcggat attacggcta cggagatgaa cgacggaggc actcctgtgg caggagaggc
     8101 ggaaagtcaa actaatctct taacagggca attcttgtcc gtaacggtgc ggttgaatca
     8161 ttattatttg tccaatccat actatggcta ctcctatgaa cgccttgtcc atacggcaga
     8221 acatgagtta ggtcatgcga ttggcttgga ccatacagat gagaagtctg tcatgcaacc
     8281 agcaggttcc ttttatggta tccaggaaga ggatgttgca aacctccgaa aaatatatga
     8341 gactagtgag tagggtacta tctttcccta ctttttttgc tataatggaa ctatgaacaa
     8401 cttgattaaa tcaaaactag agctcttgcc gaccagccct ggttgctaca ttcataagga
     8461 taaaaatggc accattatct atgtaggaaa ggctaaaaat ctgcgtaatc gagtacggtc
     8521 ctattttcgt ggaagtcatg ataccaagac agaggctctg gtgtctgaaa ttgtggattt
     8581 tgaatttatt gttacggagt ctaatattga ggcacttctc ctagaaatca acctgatcaa
     8641 ggaaaacaag cccaagtaca atatcatgct caaggatgac aagtcctatc ctttcatcaa
     8701 aatcaccaat gagcgctatc cacgcttgat tatcactcgt caggtcaaaa aggacggagg
     8761 tctttatttt ggaccctatc ccgatgtggg ggcagccaat gaaatcaagc ggttgctgga
     8821 tcggatattc ccttttcgta agtgtaccaa cccgccctct aaggtctgtt tttattacca
     8881 tatcggtcaa tgcatggccc acaccatctg taagaaggat gagacttatt tcaagtctat
     8941 ggcccaggag gtttctgatt ttctgaaagg gcaggataac aaaatcattg atgagctaaa
     9001 gggcaaaatg gcagcggcag cccagactat ggagtttgaa cgtgcggcgg aataccgtga
     9061 cctgattcag gctattggaa cgcttcgaac caagcaacgg gtcatggcta aggatctcca
     9121 aaatcgcgat gtctttggct actatgtgga taagggctgg atgtgtgtgc aggttttctt
     9181 tgtccgtcag ggtaagctca tcgagcgcga tgtcaatctc ttcccctact tcaatgatcc
     9241 agatgaggat tttttgacct atgtaggaca attctatcaa gaaaaatctc acctagttct
     9301 caatgaggta ctgattccgc aggatattga cgaagaggct gtcaaggctt tggtggatac
     9361 caagattctc aaaccccaac gtggagagaa aaaacagctg gtcaatctag ccataaaaaa
     9421 tgcacgtgtt agtctggagc agaaatttaa cctgctagag aaatctgtcg agaagaccca
     9481 aggagctatt gaaaatctag gacgtttgct ccaaatcccg accccagtac gtatcgagtc
     9541 cttcgataac tctaatatca tgggaactag ccctgtttcg gctatggtgg tctttgtcaa
     9601 cggtaaaccg agtaagaagg attaccgtaa gtacaagata aaaacggttg ttggaccaga
     9661 cgactatgcc agcatgagag aggtcattcg cagacgctat ggtcgagtac agcgtgaggc
     9721 tttgactcct ccagatttga ttgtgattga tggggggcaa ggtcaagtca atatcgctaa
     9781 gcaggttatc caagaggaac tgggcttgga tattccaatt gctgggctgc aaaagaatga
     9841 taagcaccaa acccatgaat tgctctttgg agatccgctt gaggtggtgg acttgtctcg
     9901 caattctcag gaatttttcc tcctccaacg catccaagat gaggtgcacc gctttgccat
     9961 taccttccac cgccaactgc gctccaaaaa ttctttctca tctcaattgg atgggattga
    10021 cggtctggga cctaaacgca agcagaatct tatgaagcat ttcaagtctt tgaccaaaat
    10081 caaggaagcc agtgtggatg agattgtcga agttggggta cctagagtcg ttgcagaggc
    10141 tgtgcaaaga aagttgaacc cgcagggaga agccttgcct caagtagcag aagaaagagt
    10201 agattaccaa acggaaggaa accacaatga accataaaat cgcaatttta tcagatgttc
    10261 atggcaatgc gacggcgcta gaagcagtga ttgcagatgc taaaaatcaa ggggccagtg
    10321 aatattggct tctgggagat atttttcttc ctggtccagg cgcaaatgac ttagtcgccc
    10381 tgctaaagga ccttcctatc acagcaagtg ttcgaggcaa ttgggatgat cgtgtccttg
    10441 aggctttaga tgggcaatat ggcttagaag acccacagga agttcagctc ttgcgtatga
    10501 cccagttttt gatggagcga atggatcctg caacgactgt ctggctacga agcttgcctt
    10561 tgctggaaaa gaaagaaatt gctggactgc gcttttctat ctctcataat ttgccaaata
    10621 agaactatgg tggtgacttg ctagttgaga atgatacaga gaaatttgac caactgctag
    10681 atgcggaaac ggacgtggca gtttatggtc atgttcacaa gcagttgctt cgttatggaa
    10741 gtcaagggca acaaatcatc aatccagggt cgattggcat gccctatttt aattgggagg
    10801 cgttaaaaaa tcaccgtgcc cagtatgccg tgatagaagt tgaagatggg gaattggtca
    10861 atatccaatt tcgtaaagtt gcttatgatt acgaggctga gttagaattg gccaagtcca
    10921 aggggcttcc ctttattgaa atgtatgaag aactgcgtcg tgacgataac tatcaggggc
    10981 acaatctgga acttttagcc agcttaatag aaaagcatgg gtatgtagag gatgtgaaga
    11041 atttttttga ttttttgtaa gagtttccta aaatagccaa tgcaaactaa aaaagcgatt
    11101 ggctggtcca atcgctttta gtatatctta tactcaa
//
LOCUS       DBERDK010000058        11056 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.058, whole genome shotgun sequence.
ACCESSION   DBERDK010000058 DBERDK010000000
VERSION     DBERDK010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 11056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11056
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.058"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            43..765
                     /locus_tag="ACL73L_001574"
     CDS             43..765
                     /locus_tag="ACL73L_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HGS0772527.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKAKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
     gene            762..1610
                     /locus_tag="ACL73L_001575"
     CDS             762..1610
                     /locus_tag="ACL73L_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HGS0772528.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            1642..2571
                     /gene="psaA"
                     /locus_tag="ACL73L_001576"
     CDS             1642..2571
                     /gene="psaA"
                     /locus_tag="ACL73L_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HGS0772529.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVP
                     SAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQ
                     IFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK"
     gene            2694..3185
                     /gene="tpx"
                     /locus_tag="ACL73L_001577"
     CDS             2694..3185
                     /gene="tpx"
                     /locus_tag="ACL73L_001577"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HGS0772530.1"
                     /translation="MVTFLGNPVSFTGKQLQVGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEDLDNAIM
                     LSDYFDHSFGRDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(3281..5989)
                     /locus_tag="ACL73L_001578"
     CDS             complement(3281..5989)
                     /locus_tag="ACL73L_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HGS0772531.1"
                     /translation="MIKRKTYWKDLVQSFTGSKGRFLSILILMMLGSLALVGLKVTSP
                     NMEATANAYLTTAQTLDLAVMSNYGLDQADQEELKQTEGAEVEFGYLTDVTMDNGQDA
                     IRLYSKPERISTFQLRKGRLPQSDKEIALATHLQGQYSVGQEISFKEKEEGHSSLKDH
                     TYTITGFVDSAEILSQRDMGYAGSGSGTLTAYGVILPSQFDQKVYNIARLKYQDLAGL
                     NAFSSAYEEKSKQHQEDLEQTLSDNGKVRLQLLKKEGQESLDKGQETLDKAQTNLQEG
                     KRRLAAAQARIQAQESQLALFPQVQREQASAQLTQAKQELGKEEDKLKQAEQNLAQEK
                     EKLEKHQQVLDDLAEPRYQVYNRQTMPGGQGYLMYSNASSSIRAVGNIFPVVLYAVAS
                     MVTFTTMTRFVDEERTHAGIFKALGYRSKDIITKFLLYGLVAGTVGTALGSILGHYLL
                     ASVISSVITKGMVVGETQIQFYWTYSLLALVLSLLASVLPAYLVAWRELHAEAAQLLL
                     PKPPVKGAKILLERIGFIWRRLSFTHKVTARNIFRYKQRMLMTIFGVAGSVALLFAGL
                     GIQSSVAGVPSKQFQQIQQYQMLVSENPSATNQDKVELAEVLKGQEILAYQKIYSKTL
                     DKDFKGKAGLQNITLMMIEKEDLTPFIHLQHHQQELTLKDGIVITAKLAQLAGVKVGQ
                     TLEIEGKELKVAAITENYVGHFIYMSQASYEQLYGQLPQANTYLVSLRDTSATSIESQ
                     AGLLMNQSAVSSVVQNASAIRLFDSIASSLNQTMTILVIVSVLLAIVILYNLTNINVA
                     ERIRELSTTKVLGFHNNEVTLYIYRETIVLSLVGIVLGLVAGFYLHQFLIQMISPATI
                     LFYPQVGWEVYVIPVAAVSIILTLLGFFVNYYLRKVDMLEALKSVE"
     gene            complement(5991..6692)
                     /locus_tag="ACL73L_001579"
     CDS             complement(5991..6692)
                     /locus_tag="ACL73L_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0772532.1"
                     /translation="MAYIEMKHCYKRYQVGDTEIVANCDVNFEIEKGELVIILGASGA
                     GKSTVLNLLGGMDTNDEGEIWIDGVNIADYSSHQRTNYRRNDVGFVFQFYNLVSNLTA
                     KENVELASEIVTDALNPDQVLTDVGLAHRLNNFPAQLSGGEQQRVSIARAVAKNPKIL
                     LCDEPTGALDYQTGKQVLKILQDMSRQKGATVIIVTHNGALAPIADRVIQMHDASVKD
                     VVLNQHPQDIDSLEY"
     gene            complement(6942..9353)
                     /locus_tag="ACL73L_001580"
     CDS             complement(6942..9353)
                     /locus_tag="ACL73L_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HGS0772533.1"
                     /translation="MIRNKKQDYVLAYKQPVSTTYMGWEEEALPIGNGSLGAKVFGLI
                     GAERIQFNEKSLWSGGPLPDSSDYQGGNLQDQYVFLAEIRQALEKRDYNLAKELAEQH
                     LIGPKTSQYGTYLSFGDIHIEFSQQGTTLSQVTDYQRQLNISKALATTSYVYKGTRFE
                     REAFASFPDDLLVQCFTKEGLETLDFTIELSLTCDLASNGKYEQEKSDYKECKLDITD
                     SHILMKGRVKDNDLRFASYLAWETDGDIRVWSDRVQISGASYANLFLAAKTDFAQNPA
                     SNYRKKLDLEQQVIDLVDTAKEKGYTQLKSRHIEDYQALFQRVQLDLEADVDASTTDD
                     LLKNYKPQEGQALEELFFQYGRYLLISSSRDCPDALPANLQGVWNAVDNPPWNSDYHL
                     NVNLQMNYWPAYVTNLLETVFPVINYVDDLRVYGRLAALKYAGIVSQKGEENGWLVHT
                     QATPFGWTAPGWDYYWGWSPAANAWMMQTVYEAYSFYRDQDYLREKIYPMLRETVRFW
                     NAFLHKDQQAQRWVSSPSYSPEHGPISIGNTYDQSLIWQLFHDFIQAAQELGLDEDLL
                     TEVKEKSDLLNPLQITQSGRIREWYEEEEQYFQNEKVEAQHRHASHLVGLYPGNLFSY
                     KGQEYIEAARASLNDRGDGGTGWSKANKINLWARLGDGNRAHKLLAEQLKTSTLQNLW
                     CSHPPFQIDGNFGATSGMAEMLLQSHAAYLVPLAALPDAWSTGSVSGLMARGHFEVSM
                     SWEDKKLLQLTILSRSGGDLRVSYPDIEKSVIKMNQEKIKAKCMGKDCISVATAEGDL
                     VQFYF"
     gene            complement(9406..10098)
                     /locus_tag="ACL73L_001581"
     CDS             complement(9406..10098)
                     /locus_tag="ACL73L_001581"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HGS0772534.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIEFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            complement(10633..11013)
                     /locus_tag="ACL73L_001582"
     CDS             complement(10633..11013)
                     /locus_tag="ACL73L_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772535.1"
                     /translation="MKLKTLALISGIVGLVGGILLLIGPFVLLGTAVNTAATTLNGGA
                     TAGAFSGVALLLNALKIANLVLGIIAIVYYKGDKRVGAAPSVLMIVSGGVSLIPFLGW
                     VGGILAIIGGSLFLATLKKFKSEE"
BASE COUNT         3231 a         2524 c         2065 g         3236 t
ORIGIN      
        1 ttttttttaa tgtatattaa gagacaggag gaatacaagt ttatgatacg tatcgaaaac
       61 ctcagtgtct cctacaaaga aacgttggca cttaaggata tttcactagt gctccatgga
      121 ccaacaatta ccggcatcat tggtccaaac ggcgctggga aatcaacact attaaaaggt
      181 atgctgggaa ttatcccaca tcaaggtcag gcatttctcg atgacaagga agttaaaaaa
      241 tccttacacc gaattgccta tgtcgaacaa aaaatcaata tcgactacaa ctttcccatc
      301 aaggtcaagg aatgcgtctc gttaggacta tttccctcta ttcctctctt tcgaagttta
      361 aaggctaaac attggaagaa agtgcaagag gcccttgaaa tcgtcggcct agctgactac
      421 gctgaacgtc aaattagtca actgtctgga ggtcaattcc agcgggtctt gattgccaga
      481 tgtttggtgc aggaagccga ctatatcctc ttggatgaac cctttgctgg gattgactct
      541 gtcagtgagg aaatcatcat gaatacgctg agagatttga aaaaagctgg gaagacggtt
      601 ctcatcgttc accacgacct cagcaagatt ccccactact tcgatcaagt cttacttgtc
      661 aatcgagaag tgattgcctt tggtccaaca aaagaaactt ttaccgaaac caatctaaaa
      721 gaagcttacg gtaatcaact ctttttcaat ggaggtgacc tatgattgca gaatttatcg
      781 atggattgca aaaattccat ttcctacaaa atgccttgat aacagctatt gtcgtcggga
      841 ttgtagctgg agctgtggga tgtttcatta ttctacgcgg gatgtcactc atgggagatg
      901 ccatttcaca tgctgtctta ccaggtgtag ccctctcctt catcttgggc cttgacttct
      961 ttatcggagc cattgtcttt ggattgctag ctgccatcat cattacctac atcaaaggaa
     1021 actcgattat caaaagcgat accgccatcg gcattacctt ttcttctttc ttagccctcg
     1081 gtatcatctt gattggtgtc gctaaaagtt caactgacct tttccatatc ctttttggta
     1141 atatcctggc cgtccaagat acggatatgt ttattactat gggtgtgggg gcagccatcc
     1201 tcttgttaat ctggattttc ttcaagcaac tcttgataac ttcctttgat gaactcttgg
     1261 ctaaagccat gggaatgcct gtcaatttct atcactacct tctcatggta ctcctgactc
     1321 tcgtgtctgt gacagccatg caaagtgtcg gaactatcct gattgtagcc atgctgatta
     1381 ccccagctgc aactgcttat ctgtatgcta atagtctgaa aagtatgatt ttcctttcct
     1441 caaccttcgg agctactgct tcagttttgg gactctttat tggctatagt tttaatgttg
     1501 cggcaggttc tagtatcgtg cttacagctg ctagtttctt tctcattagc ttctttatcg
     1561 ctcccaaaca acgatatttg aaactgaaaa ataaacattt gttaaaataa ggggcaaagc
     1621 cctaataaat tggaggatct aatgaaaaaa ttaggtacat tactcgttct ctttctttct
     1681 gcaatcattc ttgtagcatg tgctagcgga aaaaaagata caacttctgg tcaaaaacta
     1741 aaagttgttg ctacaaactc aatcatcgct gatattacta aaaatattgc tggtgacaaa
     1801 attgaccttc atagtatcgt tccgattggg caagacccac acgaatacga accacttcct
     1861 gaagacgtta agaaaacttc tgaggctgat ttgattttct ataacggtat caaccttgaa
     1921 acaggtggca atgcttggtt tacaaaattg gtagaaaatg ccaagaaaac tgaaaacaaa
     1981 gactacttcg cagtcagcga cggcgttgat gttatctacc ttgaaggtca aaatgaaaaa
     2041 ggaaaagaag acccacacgc ttggcttaac cttgaaaacg gtattatttt tgctaaaaat
     2101 atcgccaaac aattgagcgc caaagaccct aacaataaag aattctatga aaaaaatctc
     2161 aaagaatata ctgataagtt agacaaactt gataaagaaa gtaaggataa atttaataag
     2221 atccctgctg aaaagaaact cattgtaacc agcgaaggag cattcaaata cttctctaaa
     2281 gcctatggtg ttccaagtgc ctacatctgg gaaatcaata ctgaagaaga aggaactcct
     2341 gaacaaatca agaccttggt tgaaaaactt cgccaaacaa aagttccatc actctttgta
     2401 gaatcaagtg tggatgaccg tccaatgaaa actgtttctc aagacacaaa catcccaatc
     2461 tacgcacaaa tctttactga ctctatcgca gaacaaggta aagaaggcga cagctactac
     2521 agcatgatga aatacaacct tgacaagatt gctgaaggat tggcaaaata agcctctgaa
     2581 aaacgtcatt ctcatgtgag ctggcgtttt ttctatgccc acatttccgg tcaaatcatt
     2641 ggaaaattct gactgtttca gatacaatgg aagaaaaaag attggagtat cctatggtaa
     2701 cttttctcgg aaatcctgtg agctttacag gtaaacaact acaagtcggc gacaaggcgc
     2761 ttgatttttc tcttactaca acagatcttt ctaaaaaatc tctggctgat tttgatggca
     2821 agaaaaaagt cttgagtgtc gttccttcta tcgatacagg catctgctca actcaaacac
     2881 gtcgttttaa tgaagaattg gctggactgg acaacacggt cgtattgact gtttcaatgg
     2941 acctaccttt tgctcaaaaa cgttggtgcg gtgctgaaga ccttgacaat gccattatgc
     3001 tttcagacta ctttgaccat tctttcgggc gcgattatgc cctcttgatc aacgaatggc
     3061 acctattagc acgcgcagtc tttgtcctcg atactgacaa tacgattcgc tacgttgaat
     3121 acgtggataa tatcaattct gagccaaact tcgaagccgc aattgcagct gctaaagccc
     3181 tatagaaaaa atcctctgtc acaaagagtt ccctgctctt tgaaacggag gatttttgaa
     3241 tattagtaaa tagaagttca atcagctaaa aataactacc ttactctaca gatttcaggg
     3301 cttctaacat atcaaccttt ctcagataat aattgacgaa gaaaccaagc aaggtcaaaa
     3361 tgatgcttac tgctgccact gggattacat agacttccca gcctacctgc ggataaaaga
     3421 gaatagtcgc aggcgaaatc atttgaatca aaaattggtg taaatagaaa ccagctacca
     3481 gaccaagtac gattcccaca agggatagca caatcgtctc acggtaaatg tagagtgtga
     3541 cttcattatt atgaaaaccg agaaccttgg tagtggagag ttcacggatt ctctcagcta
     3601 cgttgatatt ggtcagattg taaaggatga caatagctaa tagaaccgat acgatgacca
     3661 agatggtcat ggtctgattg agtgagctag cgatagagtc gaagagtcga atggctgaag
     3721 cattttggac aacgctggac accgcagatt gattcataag caagcccgcc tgactttcga
     3781 tactagttgc actggtatcc cttaatgaga ccagataagt gttggcttgg ggtagctgtc
     3841 cgtaaagttg ctcatagcta gcctgactca tataaataaa gtgaccaacg tagttctcag
     3901 taatagcagc gacctttagt tccttacctt caatttctaa agtctgccca accttgacac
     3961 ctgccagctg ggcgagttta gctgtaataa cgatgccatc ttttaatgtc agctcctgct
     4021 gatgatgttg aagatggata aagggagtca aatcttcctt ctctatcatc ataagagtaa
     4081 tgttttgaag accagccttg cctttgaaat ccttgtctag cgttttagaa tagattttct
     4141 ggtaggctag tatctcctgc cctttcaata cttctgctag ctctaccttg tcctgattgg
     4201 tcgcactagg attttcagag acaagcatct gatactgttg gatttgttga aactgtttag
     4261 acggaactcc tgctacagaa gattggattc ccaaacctgc aaagagcaga gctacagaac
     4321 ctgccacacc aaagattgtc atcaacattc tctgcttata acgaaagatg ttgcgggctg
     4381 ttaccttatg agtaaaactg agacgacgcc agataaaacc gatacgctcc aataagattt
     4441 tagctccttt gacaggaggt ttaggaagta gaagctgggc tgcttcggca tgaagttccc
     4501 tccaagccac cagataggct ggtaacacac tcgccaacaa gctcaagaca agagctagta
     4561 agctataggt ccaatagaac tgaatctggg tttctcccac caccatgcct tttgtaatga
     4621 cacttgaaat tacactggct agcaaataat gaccaagtat actacctaga gccgttccga
     4681 cagtcccagc tactagtcca taaaggagaa acttggtgat aatatcctta ctacggtaac
     4741 ccaaggcctt aaaaatccct gcatgagttc gctcttcgtc tacaaagcga gtcatggtcg
     4801 taaaggtcac catggatgct acggcataaa gtaccacagg aaagatattg cccactgctc
     4861 gaatactgga tgaagcattg ctatacataa gatagccctg accacctggc atggtctgac
     4921 gattatagac ctgatacctt ggctccgcca aatcgtccaa gacttgctga tgtttttcta
     4981 atttttcctt ttcttgggct agattttgtt cagcttgctt tagtttatcc tcttccttgc
     5041 ccaattcctg cttggcttgg gtaagttgag cactagcctg ctctctctga acttgaggaa
     5101 acaaggctag ttgactttct tgagcctgta tacgagcttg agcagctgct aaacgacgct
     5161 tgccttcctg caaattagtc tgagccttgt caagggtctc ttgccccttg tctagagact
     5221 cttgtccttc ttttttcaaa agttgcagac gtaccttgcc attatctgat aaagtttgtt
     5281 caaggtcttc ctgatgttgc ttggattttt cttcataagc tgatgaaaag gcatttaaac
     5341 ctgctaaatc ttgatatttc aaacgagcta tattgtagac tttctgatca aactgactag
     5401 gtaaaatcac cccataggct gtcagagtcc cacttccact tcctgcgtag cccatatctc
     5461 gctgggagag gatttcagcc gaatccacaa aaccagtaat ggtataagta tggtctttta
     5521 aagaggaatg accctcttct ttttctttaa aactaatctc ctgtcccacg ctgtattggc
     5581 cttgcaaatg agtggccaaa gcgatttcct tgtctgactg aggaagtcgt ccctttctta
     5641 gctgaaaggt tgaaattcgc tctggtttgg agtacagccg aatggcatcc tgcccattat
     5701 ccatagtcac atctgtcaaa tagccaaact cgacctctgc gccctccgtc tgttttagtt
     5761 cttcttggtc tgcttgatcc aagccatagt tagacatgac tgccaaatcc aaggtttgag
     5821 cagttgttaa ataagcatta gctgtcgcct ccatgttggg actggttact ttgaggccta
     5881 ctaaggctag agatcccaac atcatcagga tcaagatgga taaaaaacgc cccttggagc
     5941 ctgtgaagga ctgaactaag tccttccaat aagtttttcg cttgatcatg ctagtactcc
     6001 aaactgtcaa tatcctgagg atgctggttg agcaccacat ccttgacact ggcatcgtgc
     6061 atttgaatca cgcgatcagc aatgggcgcc aaagctccat tatgagtcac gatgatcacc
     6121 gtcgctccct tttgacgaga catgtcttgg agaattttca aaacctgctt gcccgtctga
     6181 taatccaagg ctccagtcgg ttcatcacaa aggagaattt taggattttt ggctaccgcg
     6241 cgtgcaatgg agactcgctg ttgctcccct ccagaaagct gggctggaaa gttattgaga
     6301 cgatgagcca gacctacatc tgtcaagact tgatcaggat tcaaggcatc tgtcacaatt
     6361 tcagaagcca gttccacatt ttccttagct gtcagattag aaactagatt ataaaactga
     6421 aaaacaaacc ccacatcatt tctacggtaa ttggtgcgct ggtgggaact ataatccgca
     6481 atattaacac catcaatcca gatttcccct tcatcattgg tatccattcc cccaagaagg
     6541 ttaagaactg ttgacttgcc tgcacctgaa gcaccaagga taataaccag ctcccccttt
     6601 tcaatctcaa aattcacatc acaattggcc acaatctccg tgtccccaac ctgataacgc
     6661 ttgtaacagt gtttcatctc aatataagcc atcagaccca ccctctatca aacaaattat
     6721 actcctcgaa aatctctctt caaaccacgt cagcttccat ctgcaacctc aaaacactgt
     6781 tttgagcaac ctgcggctag cttcctagtt tgtgctcttt gattttcatt gagtattact
     6841 tcctattatc attataacgt tttttcaact agttgaaaac tgcttatatt cttaaatcct
     6901 tattaaaagg cagtttcaaa ttactgcctt ataacatctt cttaaaaata aaattgaaca
     6961 agatcacctt ctgctgttgc caccgaaata caatctttcc ccatgcattt cgcttttatt
     7021 ttttcttgat tcattttaat cacactcttc tcaatatctg gataagaaac tcgcaaatct
     7081 cctccactcc ttgataaaat ggtcaactgt aagagttttt tatcttccca gctcatgctc
     7141 acttcaaaat gtccacgtgc cattaagcct gaaacagaac ctgttgacca agcatcaggt
     7201 agggcagcta gaggtaccag ataagctgca tgagactgga gtaacatttc tgccatgcca
     7261 ctagtagcac caaaattacc atctatctga aaaggaggat ggctacacca aagattttgc
     7321 aaggtggatg tctttaactg ctctgccaat aatttatggg ctcgattgcc atctcccaaa
     7381 cgcgcccaga gattgatctt attagccttg gaccagcctg tgccgccatc tccacgatca
     7441 ttgaggctag cacgcgccgc ttcaatatac tcttgtccct tgtagctaaa gagattgcca
     7501 ggatagagtc ccactagatg ggaagcgtgc cgatgctggg cctccacttt ctcattttga
     7561 aaatactgct cttcctcctc ataccactcc ctgattcgac cagattgagt gatttgcaaa
     7621 ggatttagta aatcagactt ctccttaacc tcagtcaaca agtcctcatc cagtcccaat
     7681 tcctgagcag cctgaataaa atcatgaaat aactgccaaa tcagagattg gtcataggta
     7741 ttgccaatcg aaatcggccc atgttctggg gaataagacg gagaagacac ccaacgctgc
     7801 gcctgctgat ccttatgtaa aaaggcattc caaaaacgaa ccgtttccct caacatggga
     7861 taaattttct ccctaagata gtcttggtcc ctataaaatg aataggcttc ataaacggtt
     7921 tgcatcatcc acgcattggc agctggtgac caaccccaat agtaatccca accaggtgcc
     7981 gtccaaccaa agggagtcgc ttgagtatga accaaccaac cattttcctc acctttctga
     8041 gagacgattc ctgcatactt taaagccgct agacgaccat agacacgcaa atcatctaca
     8101 tagttgatga ctggaaagac cgtctctaga agattggtaa cataggctgg ccaataattc
     8161 atctgcagat tgacatttaa gtgatagtcc gaattccaag gaggattgtc gaccgcattc
     8221 cagactccct gtaggttagc tggtagagca tctgggcagt ctctggacga actaatcaat
     8281 aaataccgtc catactggaa gaacagctcc tccaaagcct gcccttcttg tggcttataa
     8341 ttttttaaca aatcatctgt agtggatgcg tcaacatcag cttccaaatc caattgaaca
     8401 cgctggaata aggcttggta gtcctcgata tgccttgatt tcaattgggt atagcccttt
     8461 tctttagctg tgtccaccaa gtctatcacc tgttgctcta aatctagttt cttgcgataa
     8521 ttgctagcag gattttgggc aaaatccgtc ttagcggcca agaagagatt ggcataactg
     8581 gctcctgata tctgaaccct atctgaccaa actctaatat ctccatccgt ttcccaagct
     8641 agataactag caaaccgcag atcattatcc ttaactcttc ccttcatcaa gatatgagaa
     8701 tcagtaatat ccaacttaca ctccttgtaa tcagattttt cctgctcata ctttccatta
     8761 gaagccaaat cacaggtcaa ggatagttct atagtaaaat ctagagtttc caacccttcc
     8821 ttagtaaaac attgaaccaa gagatcatct ggaaaactcg caaaagcttc acgttcaaat
     8881 cgcgttccct tatagacata agaagtcgtc gcaagtgcct tactaatatt caactgtctc
     8941 tgatagtccg tcacctgaga caaagtcgta ccttgctggc tgaactcaat gtgaatatcc
     9001 ccaaaagaca gataggtccc atattgactc gtttttggcc caattaggtg ctgctcagcc
     9061 agttccttag ccagattgta atctctcttc tccaaagcct gccgaatctc agctaaaaaa
     9121 acatactgat cctgaagatt tccaccctga taatctgaac tatcaggaag tggacctcca
     9181 gaccagagac ttttttcatt aaattgaatc cgttcagccc ctataaggcc aaatactttt
     9241 gctcctaaag aaccattacc tatcggtaaa gcttcttctt cccaacccat gtaagtggtt
     9301 gaaactggtt gcttgtaggc cagtacataa tcttgttttt tattccttat catacttcca
     9361 tactaacata aaaagcctag aaatcaatcc taggcttaca attttttatt ttccaaggta
     9421 gtattcagaa acaacgttca atttttcgtc gaattcgaat accaatggtg ggaagttagg
     9481 gatttccacg tccatgatct catcatctga caaacctttg atgtgtttta caagggcacg
     9541 gattgagtta ccgtgagctc ctacgaatac gtttttacca tctttaagag ctggagcgat
     9601 tttatcttcc cagaatggaa gagcacgttc caaagtcact ttcaagtttt cagcatctgg
     9661 gataactgag tcgtcaagtg aagcgtaacg acggtctgtg tgagctgagt gctcatcatc
     9721 acggtccatg tttggaggca atacatcgta tgaacgacgc cagatgtgaa cttgctcatc
     9781 accaaattgt tcagcagctt cagctttgtt tttaccagtc aaaccaccgt agtgacgttc
     9841 gttcaagcgc catgattttt caactggaac ccacaattgg tcagaagctt caagagccaa
     9901 gttagttgtt ttgatagcac gtttcaatac tgaagtgtaa gcttggtcaa attcgatacc
     9961 agcttctttg atcaatttac cagcgtcaat cgcttgttgt gtaccttttt cagacaaatc
    10021 aacatcagcc caaccagtga aaaggttagc tttgttccat tcagactcac cgtggcgagc
    10081 aaaaaccaat tttaccatta gatggattct cctttaaatt ttccgaggtt tcccctcgtt
    10141 tatctattct attttacaca atttttcaca aaaaagctag tcaaaaccaa aaaggaaagg
    10201 actgtttgac aatgtttggc aatcacattg tttttggttg tttctattct atcttcatta
    10261 tttaaccttt ttttctgcgc atcgaataat ttttgagttg actcttttag tttagtaaac
    10321 gagtcattat tttccatagc tgtgcctttg aaaccttcct cagctttagt aaaataatca
    10381 tcaaaaatat ttttataaag atctatataa tctgcataag tgcgtattga ttcaatcgca
    10441 ttgtctgtta caaccggtgt atcgaaagtg attgtttctt ttgaactatt ggatgttgct
    10501 actgtcttct catcattact tgttgaagta ttctttgaac tttatgttcc acatgcaact
    10561 agaaaagaaa gagataatag agctactcct ataccgataa acttttttgt tcttttcatg
    10621 ctaaaatacc ttttattctt ctgatttgaa tttcttcaat gttgcaagga atagagatcc
    10681 tccgataata gcaagaatcc ccccaaccca tcctaagaat ggaatgagac taactccacc
    10741 agaaacaatc attagtacag acggagctgc acctacacgc ttatctcctt tatagtaaac
    10801 aatagcaatg ataccaagaa caagatttgc aatcttcaag gcattcaaga gtaaggcaac
    10861 acctgaaaaa gcccctgcag tagctcctcc attaagagtt gtagcagctg tgtttaccgc
    10921 tgttcccaac aagacaaaag gaccaataag aagtaaaatt cctcccacaa gaccgacgat
    10981 accactaatt aaagcaagtg tttttaattt cataataaaa tctccttttt tatttttttc
    11041 aaataaaatt gtatca
//
LOCUS       DBERDK010000059        10949 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.059, whole genome shotgun sequence.
ACCESSION   DBERDK010000059 DBERDK010000000
VERSION     DBERDK010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 10949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10949
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.059"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(103..897)
                     /locus_tag="ACL73L_001583"
     CDS             complement(103..897)
                     /locus_tag="ACL73L_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HGS0772536.1"
                     /translation="MKKFSLLLAILPFLVACENQATPKETSAQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAEKYLYSLPISNNPLVLVSNKKNPLTSLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
                     NPATINFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSADSPSN
                     YIIFSSDQKEFKEQFDKALKELYQDGTLEKLSNTYLGGSYLPDQLQ"
     gene            1068..1673
                     /locus_tag="ACL73L_001584"
     CDS             1068..1673
                     /locus_tag="ACL73L_001584"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HGS0772537.1"
                     /translation="MKFLELNKKRHATKHFTDKLVDPKDVRTAIEIATLAPSAHNSQP
                     WKFVVVREKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYSEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIEDRFRPELLITVGYTDEKLEPSYRLPVDEIIEKR"
     gene            1686..3086
                     /gene="pepV"
                     /locus_tag="ACL73L_001585"
     CDS             1686..3086
                     /gene="pepV"
                     /locus_tag="ACL73L_001585"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HGS0772538.1"
                     /translation="MTAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGP
                     GPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGAARLHSFTGGLREN
                     MVPESATAVVSGDLADLQAKLDAFVAEHKLRGELQEEAGKYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFGFAGPAKDYLDIAGKILLNDHEGENLKIAHVDEKMGALSMNAGV
                     FRFDETSADNTIALNIRYPKGTSPEQIKSILENLPVASVSLSEHGHTPHYVPMEDPLV
                     QTLLNVYEKQTGLQGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            3140..3718
                     /locus_tag="ACL73L_001586"
     CDS             3140..3718
                     /locus_tag="ACL73L_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HGS0772539.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFAAPKTARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPRT
                     GFAEKWHPQLLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKHYKDYLPAYFPLV
                     HPSPRNQIWMAKNPWFEAEVVPDLKKRIKTIL"
     gene            3878..4165
                     /locus_tag="ACL73L_001587"
                     /pseudo
     CDS             3878..4165
                     /locus_tag="ACL73L_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
     gene            4336..5661
                     /gene="brnQ"
                     /locus_tag="ACL73L_001588"
     CDS             4336..5661
                     /gene="brnQ"
                     /locus_tag="ACL73L_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HGS0772540.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRILTPVFAILIVIL
                     VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVVGIVVALAFSALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIASVLGS
                     SFKVEFLANLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            5759..6796
                     /locus_tag="ACL73L_001589"
     CDS             5759..6796
                     /locus_tag="ACL73L_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HGS0772541.1"
                     /translation="MNQTVEYIKELTAIASPTGFTREISNYLVKTLEGFGYQPVRTAK
                     GGVNVTIKGQNDEQHRYVTAHVDTLGAIVRAVKPDGRLKLDRIGGFPWNMIEGENCTV
                     HVASTGEKVSGTILIHQTSCHVYKDAGTAERTQDNMEVRLDAKVTNEKETRALGIEVG
                     DFISFDPRTVVTETGFIKSRHLDDKVSAAILLNLLRIYMEEKIELPVTTHFAFSVFEE
                     VGHGANSNIPAQVVEYLAVDMGAMGDDQQTDEYTVSICVKDASGPYHYDFRQHLVTLA
                     KNQDIPFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVVATERM
                     VDAYLRSNLVD"
     gene            6798..7268
                     /locus_tag="ACL73L_001590"
     CDS             6798..7268
                     /locus_tag="ACL73L_001590"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HGS0772542.1"
                     /translation="MCLICQRIELIKKGENPYFVKELETGYLVIGDHQYFAGYSLFLA
                     KEHVTELHHLKKETRLRFLEEMSLVQEAVAKAFAAEKMNIELLGNGDAHLHWHLFPRR
                     RGDMNGHGLKGCGPVWWVPFEEMTAETCQVKPEEIKRLVECLSSEVDKLLEIKE"
     gene            7271..7987
                     /gene="ldcB"
                     /locus_tag="ACL73L_001591"
     CDS             7271..7987
                     /gene="ldcB"
                     /locus_tag="ACL73L_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase LdcB/DacB"
                     /protein_id="HGS0772543.1"
                     /translation="MKKRYLVLTALLALSLAACSQEKAKNEDGTAKTEQTAKADGTVG
                     SKSQGAAQKKAEVVNKGDYYSIQGKYDEIIVANKHYPLSKDYNPGENPTAKAELVKLI
                     KAMQEAGFPISDHYSGFRSYETQTKLYQDYVNQDGKAAADRYSARPGYSEHQTGLAFD
                     VIGTDGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AASGLSLEEYYGFEGGDYVD"
     gene            8296..8721
                     /gene="rplK"
                     /locus_tag="ACL73L_001592"
     CDS             8296..8721
                     /gene="rplK"
                     /locus_tag="ACL73L_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HGS0772544.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFVTKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAANVESAMRMIEGTARSMGFTVVD"
     gene            8929..9618
                     /gene="rplA"
                     /locus_tag="ACL73L_001593"
     CDS             8929..9618
                     /gene="rplA"
                     /locus_tag="ACL73L_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HGS0772545.1"
                     /translation="MAKKSKQLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRAGNVQAIIGKVSFEAEKLVENFKAFNETIQKAKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
     gene            9891..10049
                     /locus_tag="ACL73L_001594"
                     /pseudo
     CDS             9891..10049
                     /locus_tag="ACL73L_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(10159..10350)
                     /locus_tag="ACL73L_001595"
     CDS             complement(10159..10350)
                     /locus_tag="ACL73L_001595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772546.1"
                     /translation="MKTKEQTRKLAAGCSKHCFEVMDRTDVVSSKHCFEVVDRTDEVS
                     NHTHGKATLTWFEEIFKEY"
     gene            10364..10726
                     /locus_tag="ACL73L_001596"
     CDS             10364..10726
                     /locus_tag="ACL73L_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HGS0772547.1"
                     /translation="MLEVGLNSLISLFTFTFDILYPIVKVGHTDDYSHGAVKLSVSLV
                     DKDAMKKISVTVIGYFEINIDENITDILYVNGTAILYHYLRSIVSIVSAIDSSEAMLL
                     PTINVLELLDKSQPFEEE"
BASE COUNT         3255 a         2116 c         2439 g         3139 t
ORIGIN      
        1 aaaatcaaag agcaaactag gaagctagcc gtaggttgct caaagcacag ctttgaggtt
       61 gcagataaag ctgacgtggt ttgaagagat tttcgaagag tgttattgta attgatctgg
      121 gaggtaagaa ccacctagat aggtattgct gagtttttca agggttccgt cttgatagag
      181 ttctttgagc gctttatcaa attgctcttt aaactctttt tggtcgcttg agaaaatgat
      241 ataattgctg gggctatctg cagaaggtaa atcaacgact gagaggtcta aaccacggtc
      301 cttgataatc ttttgaacgg ataccttgtc aaaaactagg aaatcaaact ctccgttagc
      361 aaggtctagg attcgtttac caatatcctc accagaaaaa ttaattgtag cgggattatc
      421 agtgtgtttc tgattccagt tattgatgaa ttgagcgtta gaagttccgg tatcctcttg
      481 tgttgtttta ccagcgatct ggtcaagaga agtcaaagga tttttcttgt tgctgacaag
      541 gacgagggga ttgttggaaa tcggaagcga gtaaaggtat ttttcagcac gctcttttgt
      601 gtaactcaag ttattggccg cagcctgata gtgaccagaa tcaagtcctg ggaagatgct
      661 ctcccaggcg gttctttgga attgaatctc gtagtcgctg agtttttcat ctactgcctt
      721 taaaacttcg atatcaaagc ctgtcagatt gcccttgtct tcgtagtcaa atggtggcac
      781 gtcgccagct gtagcaagga cgattgtctt ttgagcgcta gtctctttgg gtgtagcttg
      841 attctcacag gcaaccaaaa atggtaggat agctagtaat aggctaaatt ttttcatact
      901 gtctccattc aaatgtaaag tacttgctag tttatcagaa actttcctga taaaccaata
      961 tatatctttt atgtctgtga tagaaaaact taatcatgaa aaatccctgc aagctctttc
     1021 aaattatggt agaatggaag cagtagaatt agaaaaggaa atcgattatg aaatttcttg
     1081 aattaaataa aaaacgtcat gcgactaagc attttactga taagcttgtt gatcccaaag
     1141 atgtgcgtac ggctatcgaa attgcaacct tggcgccaag cgcccacaac agccagcctt
     1201 ggaaatttgt ggtggtacgt gagaaaaatg ctgaactggc aaagttagct tatggttcca
     1261 attttgaaca ggtatcatca gcgcctgtaa ccattgcctt gtttacagat acggacttag
     1321 ccaaacgtgc tcgtaagatt gcccgtgttg gtggtgctaa taacttttct gaagagcaac
     1381 ttcaatattt tatgaaaaat ctgccagctg agtttgcccg ttacagtgag caacaagtca
     1441 gcgactacct agctctcaat gcaggtttgg ttgccatgaa cttggttctt gcattgacag
     1501 accaaggaat tggttctaac attattcttg gttttgacaa atcaaaagtt aatgaagttt
     1561 tggaaatcga agaccgtttc cgcccagaac tcttgatcac agtgggttat acagacgaaa
     1621 aattggaacc aagctaccgc ttgccagtgg atgaaatcat cgagaaaaga tagaaagaag
     1681 aaaaaatgac agcaattgat tttacagcag aagtagaaaa acgcaaagaa gacctcttgg
     1741 ctgacttgtt tagccttttg gaaatcaatt cagaacgtga tgacagcaag gctgatgccc
     1801 agcatccatt tgggcctggt ccagtaaaag ccttggagaa attccttgaa atcgcagacc
     1861 gcgatggcta cccaactaag aatgttgata actatgcagg acattttgag tttggtgatg
     1921 gagaagaagt tctcggaatc tttgcccata tggatgtggt gcctgctggt agcggttggg
     1981 acacagaccc ttacacacca actatcaaag atggtcgcct ttatgcgcgc ggggcttcgg
     2041 acgataaggg tcctacaaca gcttgttact atggtttgaa aatcatcaaa gaattgggtc
     2101 ttccaacttc taagaaagtt cgcttcatcg ttggaacaga cgaagaatca ggctgggcag
     2161 acatggacta ctactttgag cacgtaggac ttgccaaacc agatttcggt ttctcaccag
     2221 atgctgaatt tccaatcatc aatggtgaaa aaggaaatat cacggaatac ctccactttg
     2281 caggagaaaa tacaggtgct gcccgtcttc acagctttac aggtggttta cgtgaaaata
     2341 tggtaccaga atcagcaaca gcagtcgttt caggtgattt ggctgacttg caagctaaac
     2401 tagatgcctt tgttgcagaa cacaaactta gaggagaact ccaagaagaa gctggcaaat
     2461 acaaggtgac gatcattggt aaatcagccc acggtgctat gcctgcttca ggtgtcaatg
     2521 gcgcaactta ccttgccctc ttcctcagcc agtttggctt tgctggtcca gccaaagact
     2581 accttgacat cgcaggtaaa attctcttga acgatcatga gggtgaaaat ctcaagattg
     2641 ctcatgtgga tgaaaagatg ggtgctcttt ctatgaatgc cggagtcttc cgcttcgatg
     2701 aaacaagtgc tgataatacc attgccctta acatccgcta tccaaaagga acaagtccag
     2761 aacaaatcaa atcaatccta gaaaacttgc cagttgcttc tgtcagtcta tcagaacatg
     2821 gtcatacccc tcactatgtg ccaatggaag atccacttgt gcaaaccttg ttgaatgtct
     2881 atgaaaaaca aacaggccta caaggtcatg aacaagtcat cggtggtgga acctttggtc
     2941 gcttgttaga gcgcggagtt gcctatggtg ctatgttccc agactcaatt gataccatgc
     3001 accaagccaa tgaatttatt gccttggacg atctcttccg agcagcagca atttatgccg
     3061 aagctattta cgaattgatc aaataaaacg atagaagtct gagatcttat gcttggactt
     3121 ctttttggag ggaaagtaga tgtctcaaat cgaaagaatc aaacaggcta tcatggcgga
     3181 ttcgcagaat gccagctata cagagcgtgg cattgagcct ctctttgcag cgccaaaaac
     3241 tgctcgcatc aatatcatcg gtcaggctcc gggacttaaa actcaagaag caggccttta
     3301 ctggaaagat aaaagtggtg accgcttgcg ggactggcta ggtgtggatg aagatacctt
     3361 ttacaattca ggttattttg ctgttttgcc tatggatttc tactttccag gacatggcaa
     3421 gtcgggtgat cttccgcctc gtacaggttt tgcagaaaaa tggcatccgc agctcttaca
     3481 ggaattgcct gatattcagt taaccctctt gattgggcaa tatgcccaag cctactattt
     3541 acaggagaaa atcagtggga aggtaacgga gagggtgaaa cactataaag actatctgcc
     3601 agcctatttt ccgctagttc acccatcacc acgaaatcaa atctggatgg ccaaaaatcc
     3661 ttggtttgag gcagaagtag tgccagattt gaaaaaaaga attaaaacca ttttatagtc
     3721 aatgaaaatc aaagagcaaa ctaggaagct agtcgtaggc tgctcaaagt acagctttga
     3781 agttgcagat aaaactgacg aagtcggtaa catacgcacg gtaaggcgac gctgacgtgg
     3841 tttgaagaga ttttcgaaga gtattagaag aaaaagaatg aaagaaatag cctttgacgc
     3901 attttaccag ctttaccaaa acgaccagct ttctttagtg gatgtgagag aagtggatga
     3961 gtttgcagct cttcatttag aaggtgccta caacctaccg cttagtcaat tggctgatag
     4021 ttatgattaa ttggacaaag atcgcttgca ttatattatt tgcaaatctg gaatgagatc
     4081 ggcgcgtgct tgccaattcc tattagaaca aggttataat gttatcaatg tccagggtgg
     4141 catgttagcc tttgaagaac tttaaaattt tgcatttctc ctacttggtg tggactgggt
     4201 aggagagttt tatttttaga taattcttat ttttaagaaa attgaaaaca tttaatattt
     4261 gcctcgtgat gcttttttca gactcctaat cgtggtatac taggtcagta ttttataaat
     4321 atgaaggaga tttttatggc taaaaaaggt accctaacag gtttgctcct gtttggaata
     4381 ttttttggtg cggggaactt gatttttccg ccttctctag gtgctctatc tggagaacat
     4441 tttcttcctg ccatcgcagg ttttgtcttt tcaggcgttg gtatcgccgt cttgaccctt
     4501 attattggaa cgctaaatcc taaaggatat atctacgaga tttcaacgaa gatagcgcct
     4561 tggtttgcga ctctttacct ctcagttctt tacttgtcaa tcggtccatt ctttgctacc
     4621 ccacgtactg ctacaacagc ttacgaagta gggattagcc cccttttgtc ggatgcaaat
     4681 aaaggacttg gcttgattgt atttacggtt ctgtattttg cggcagccta tttgatttcg
     4741 cttaatccat caaaaatctt agaccgcatt ggacgtattt taacgccagt ctttgcaatt
     4801 ttgattgtta tcttggtcgt tctgggagct atcaaatatg gtggaacaag tcctcaagct
     4861 gcttcagctg cttatcaagc ttctgccttt ggtacaggtt tcctagaagg ttataatacc
     4921 ttggacgccc ttgcctcagt ggcctttagc gtaatcgcag ttcaaacctt gaaacaactt
     4981 ggattttcaa gtaagaaaga atacatttca actatttggg ttgttggtat cgttgttgcc
     5041 cttgccttca gcgctcttta catcggttta ggttttcttg gaaatcattt cccagtacca
     5101 gctgaagcga tgaagggtgg aacaccaggt gtttacatct tgtcacaagc cactcaagaa
     5161 atctttggct caacagctca actcttcctt gcagctatgg ttaccgtaac ctgcttcaca
     5221 acgactgttg gtttgattgt gtcaacagct gagttcttta atgagcgctt cccacaaatc
     5281 agctacaagg tttatgcgac agcctttacc ttgattggat ttgctattgc caatttgggt
     5341 cttgatgcga ttatcaagta ctcaattcca gtactggtta tcttgtaccc aatcacgatt
     5401 gctatcgtta tgattgtcat tgtcaacaaa tttgttgccc tttcaaaacc aggtatgcag
     5461 ttgacaattg ctgtggttac agttattgcc attgcaagcg tactaggaag ctcgtttaag
     5521 gttgagtttc ttgcaaatct tgttagcgtt cttccttttg ccaaggcatc tctcccatgg
     5581 ttggtgccag ccattgttgg aatcttgctc tcattggttc taccaaacaa gcaagaaagc
     5641 gatgtttttg aaatggaata atcacttaaa tcacttttgt agccaagtct acaggagtga
     5701 ttttcttttt ttatccgatg ataaatgtgt tataataggt agcgaaagag gtgaagaaat
     5761 gaatcaaaca gtagaatata tcaaagaact gacagccatc gcatcgccaa cgggctttac
     5821 tcgtgaaatt tcgaattatt tagtcaagac tctagaaggt tttggttacc agccagttcg
     5881 cacagctaag ggtggtgtca atgtgaccat caaaggtcaa aatgatgagc aacatcgcta
     5941 tgtgactgct catgtggata cgctgggtgc tattgttcgt gctgtcaaac cagatggtcg
     6001 tcttaaattg gaccgtatcg gtggttttcc ttggaacatg attgaaggtg aaaactgtac
     6061 cgttcatgtg gctagcacag gtgaaaaagt atcaggcacc atcctcatcc accaaacttc
     6121 ttgccatgtc tacaaggatg caggaactgc agaacgtacc caggacaata tggaagtgcg
     6181 tttggacgcc aaagtaacca atgaaaaaga aactcgtgcc ttggggattg aggtcggtga
     6241 ttttatcagt tttgatccac gaactgtcgt gacagagaca ggttttatca agtctcgtca
     6301 tttggatgac aaggtcagcg cagcgatttt gcttaacctg cttcgtattt atatggaaga
     6361 gaagattgag ttgccagtaa caacccattt tgccttttca gtctttgaag aagtggggca
     6421 tggtgctaat tctaatattc ctgctcaagt agtcgaatat ctggctgtgg atatgggagc
     6481 tatgggggat gatcagcaaa cagatgagta cacagtgtcc atctgtgtca aggatgcttc
     6541 aggtccttat cactatgact tccgtcaaca tttggtgacc ttggctaaaa accaagatat
     6601 tccattcaag ctggatatct atccatttta tggttcggac gcttcagcgg ctatgtctgc
     6661 aggtgcagaa gttaaacacg ctcttcttgg tgcgggtatc gagtctagtc attcttatga
     6721 gcgcactcat attgactcgg tggtcgcaac agaacgaatg gtcgatgcct atcttagaag
     6781 caatttagta gattgatatg tgccttattt gccagagaat tgagctcatc aagaagggag
     6841 aaaatcccta ctttgtcaaa gagttggaaa caggctatct tgtgattgga gaccaccagt
     6901 attttgcagg atatagtctt tttctagcta aggaacacgt taccgaattg caccatttga
     6961 aaaaggaaac aagactccgt tttctcgaag aaatgagtct agtccaagag gcagttgcca
     7021 aggcatttgc tgctgagaaa atgaatatcg aactgctagg aaatggcgat gcccatctcc
     7081 actggcacct tttccctaga cgaagaggtg atatgaatgg ccacggtctc aagggctgtg
     7141 ggccagtttg gtgggttccc tttgaagaaa tgacagcaga aacttgccaa gtaaaaccgg
     7201 aagagattaa aagattagtc gaatgtttat cgtcagaagt agataaacta ttagaaataa
     7261 aggagtagaa atgaaaaaaa gataccttgt cttgacagcc ttgctagcct tgagtctagc
     7321 agcttgttca caagaaaaag caaaaaatga agatggcaca gctaagacag aacagacagc
     7381 caaagctgat gggacagtcg gcagtaagtc tcaaggggct gcccagaaaa aagcagaagt
     7441 ggtcaataaa ggtgattact acagcatcca aggaaaatac gacgaaatca tcgtagccaa
     7501 taaacattat cctttgtcga aagactataa tcccggggaa aatccaacag ccaaggctga
     7561 gttggtcaaa ctcatcaaag cgatgcaaga ggcaggtttc cctattagtg atcattacag
     7621 tggttttaga agttatgaaa ctcagaccaa gctctatcaa gattatgtca accaagatgg
     7681 aaaggcagca gctgaccgtt actctgcccg tcctggctat agcgaacacc agacaggctt
     7741 ggcctttgat gtgattggga ctgatggtga tttggtgaca gaagaaaaag cagcccaatg
     7801 gctcttggat catgcagctg attatggctt tgttgtccgt tatctcaaag gcaaggaaaa
     7861 ggaaacaggc tatatggctg aagaatggca cctgcgttat gtaggaaaag aagctaaaga
     7921 aattgctgca agtggtctca gtttggaaga atactatggc tttgaaggcg gagactacgt
     7981 cgattaatac tcttcgaaaa tctcttcaaa ccacgtcagc gtcgccttac ctactgactg
     8041 cgtcggttct attcacaacc tcaaaacagt gttttgagct gacttcgtca gttttatctg
     8101 caacctcaaa gctgtacttt gagcagcctg cggctagctt cctagtttgc tctttgattt
     8161 tcattgagta caaaaaataa acttttctct tgcaattcca gataaatagt gtataatgga
     8221 tgggtatgtg aaaaacatac ttgtgggagg taaaaatctc taattaccgc caaaaccaca
     8281 aaggaggatt taaaaatggc taaaaaagtc gaaaaacttg taaaattgca aatccctgct
     8341 ggtaaagcta caccagctcc accggttgga cctgctcttg gtcaagctgg tatcaacatc
     8401 atgggattca caaaagagtt caacgctcgt acagctgacc aagctggtat gatcattcca
     8461 gttgttatct cagtttacga agataaatca tttactttcg ttacgaaaac accaccagct
     8521 gctgttcttt tgaaaaaagc tgcaggtgtt gaaaaaggat caggtacacc taataaaact
     8581 aaagttgcta cagttactcg tgcacaagta caagaaattg cagaaactaa gatgccagat
     8641 ttgaacgcag caaacgtaga gtctgcaatg cgtatgatcg aaggtactgc tcgttctatg
     8701 ggattcactg ttgttgacta atcaataaca cagtagaaaa tctcacagca gtctattagt
     8761 tgcttttcat actaggcaag tgactgagcc ttgtacttgg gtacagcaag ggagcttaag
     8821 gccgtaggaa gagaaaaata gtagactgaa aacccgcaag acttcatcat ttcgagaagt
     8881 gacgtgggag atgaaaatcg attgaaccac ttacaaggag aatagaaaat ggctaaaaaa
     8941 agcaaacaac ttcgtgctgc tcttgagaaa atcgacagca caaaagcata cagcgtagaa
     9001 gaagctgtag cacttgcaaa agaaactaac tttgcaaaat ttgacgcaac tgtagaagtt
     9061 gcttacaact tgaacatcga cgttaaaaaa gctgaccaac aaatccgtgg agcaatggta
     9121 ttgccaaacg gtactggtaa aacttcacgt gttcttgttt tcgcacgtgg tgcaaaagct
     9181 gaagaagcaa aagctgctgg tgcagacttt gttggtgaag atgaccttgt tgctaaaatc
     9241 aacgacggtt ggttggactt cgacgtagtt atcgctacac ctgatatgat ggctcttgtt
     9301 ggacgtcttg gacgtgtcct tggaccacgt aacttgatgc caaaccctaa aactggtact
     9361 gtaacaatgg atgttgctaa agcagttgaa gagtctaaag gtggtaaaat cacttaccgt
     9421 gctgaccgtg caggtaatgt tcaagcaatc atcggtaaag tatcatttga agctgaaaaa
     9481 ttggttgaaa actttaaagc tttcaacgaa acaatccaaa aagcaaaacc agctacagct
     9541 aaaggaactt acgtaacaaa cttgactatc acaactactc aaggtgttgg tatcaaagtt
     9601 gacgtaaact cactttaatc agtagttaaa gtaatgtaaa aaagttgaag acgctatgtc
     9661 tcaacttttt ttgatgtacg acgggcatgt cgtatatctg aggtgtaagt cctcggtggg
     9721 cacctactac cggtgaaccc aatagcgatt cccaagtctg actatcgtga ggtagcggat
     9781 taaaacggtc tggggataga ccgttttaag tctgacgctg gaaataagaa tcgtcagagg
     9841 aagggatagc gaaatcgtgg ctctacgaac aggaacgtga taataaggcc tatatagcgg
     9901 ataaggtatc tggctggggc gactactatc aattagtagc tcagaagtcg gtacttaaac
     9961 gtgctatatc aaaaccagtc cttgaaaaac gtggactggt ttcgtgtttg gattattacc
    10021 ttgaacgaca tgcgttaaaa gttagttgaa ccgccgtatg ccgaacggca cgtacggtgg
    10081 tgtgagaggg gctagagatt atcccctact cgatttcgaa atctagtgga atgaatctgg
    10141 aatagtccat cgagctttct aatactcttt gaaaatctct tcaaaccacg tcaacgtcgc
    10201 cttgccgtgt gtatggttac tgacttcgtc agttctatcc acaacctcaa aacagtgttt
    10261 tgagctgact acgtcagttc tatccataac ttcaaaacag tgttttgagc aacctgcggc
    10321 tagtttccta gtctgctctt tggttttcat tgagtataac acattgttag aagttggttt
    10381 aaattcccta atcagtttgt tcacatttac cttcgatata ttatatccca tagttaaggt
    10441 tggtcataca gatgattata gtcatggagc cgtaaaactt agtgtttctt tagttgacaa
    10501 agatgccatg aaaaaaatat ctgtaactgt aataggatat tttgaaataa atatagatga
    10561 aaatatcacc gatattctat acgtaaatgg tactgctatt ctttatcatt atttacgttc
    10621 tattgtttca atagtttcgg caattgatag cagtgaagca atgttgctac ctaccattaa
    10681 tgttttagag ttactagata aatctcaacc ttttgaagaa gaataattta ttagctcact
    10741 aaattgaggg taaggaaaag taaaatcagt aagaaaaatg tcttgcatta tacagcaacc
    10801 ttttgggaat gagtagatgg attgaataaa attttattaa gagtggatga tttatctgta
    10861 gattattatt ggacagttag tcttgaagta gtctaagaat taggttataa tcagtagaat
    10921 ccttgctaat aatgaggagg ttagtttat
//
LOCUS       DBERDK010000060        10925 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.060, whole genome shotgun sequence.
ACCESSION   DBERDK010000060 DBERDK010000000
VERSION     DBERDK010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 10925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10925
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.060"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            1..894
                     /locus_tag="ACL73L_001597"
                     /pseudo
     CDS             1..894
                     /locus_tag="ACL73L_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1022..2044)
                     /gene="cbpF"
                     /locus_tag="ACL73L_001598"
     CDS             complement(1022..2044)
                     /gene="cbpF"
                     /locus_tag="ACL73L_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpF"
                     /protein_id="HGS0772548.1"
                     /translation="MKLLKKMMQVVLATFFFGLLGTSTVFADDSVGWQFVQENGRTYY
                     KKGDLKETYWRVIDGKYYYFDPLSGEMVVGWQYIPAPHKGVTIGPSPRIEISLRPDWF
                     YFGQDGVLQEFVGKQVLEAKTATNTNKHHGEEYDSPAEKRVYYFEDQRSYHTLKTGWI
                     YEEGHWYYLQKDGGFDSRINRLTVGELARGWVKDYPLTYDEEKLKAAPWYYLDPATGI
                     MQTGWQYLGNKWYYLHSSGAMATGWYKEGSTWYYLDAENGDMRTGWQNLGNKWYYLRS
                     SGAMATGWYQEGSTWYYLNASNGDMKTGWFQVNGNWYYAYDSGALAVNTTVGGYYLNY
                     NGEWVK"
     gene            complement(2063..2884)
                     /gene="cbpG"
                     /locus_tag="ACL73L_001599"
     CDS             complement(2063..2884)
                     /gene="cbpG"
                     /locus_tag="ACL73L_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpG"
                     /protein_id="HGS0772549.1"
                     /translation="MNVEGRGSANFIKDNVLITAAHNYYRHDYGKEADDIYVLPAVSP
                     SQELFGKIKVKEVRYLKEFRNLNSKNAREYDLALLILEEPIGAKLGTLGLPTSQKNLT
                     GITVTITGYLSYNFKIHQMYTDKKQVLSDDGMFLDYQVDTLEGSSGSAVYDASHRVVG
                     VHTLGDGANQINSAVKLNERNLPFIYSVLKGYSLEGWKKINGSWYYYRQHDKQTGWQE
                     INDTWYYLDSSGKMLTDWQKVNGKWYYLNSNRAMVTGSQTIDGKVYNFASSGEWI"
     gene            complement(2965..3204)
                     /locus_tag="ACL73L_001600"
     CDS             complement(2965..3204)
                     /locus_tag="ACL73L_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772550.1"
                     /translation="MKKTVYKKLGISIIASTLLASQLSTVSALSVISSTGEEYEVSET
                     LEKGPESNDSSLSEISPTYGSYYQKQSEVLSVMMI"
     gene            complement(3354..3899)
                     /locus_tag="ACL73L_001601"
                     /pseudo
     CDS             complement(3354..3899)
                     /locus_tag="ACL73L_001601"
                     /inference="COORDINATES: protein motif:HMM:NF020296.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(3881..4122)
                     /locus_tag="ACL73L_001602"
                     /pseudo
     CDS             complement(3881..4122)
                     /locus_tag="ACL73L_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812384.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(4123..4755)
                     /locus_tag="ACL73L_001603"
     CDS             complement(4123..4755)
                     /locus_tag="ACL73L_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HGS0772551.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYML
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGHEEF"
     gene            complement(4769..5764)
                     /locus_tag="ACL73L_001604"
     CDS             complement(4769..5764)
                     /locus_tag="ACL73L_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGS0772552.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLHDVEKTEK
                     FVFLLLVFSMSMTCLLALFWRGIEELSLRKMQANLKRLLAGQEVVQVVDQDLDASFKS
                     LSGKLNLLTEALQKAENHSLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQ
                     QALKLDREKMQTQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKLPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFQLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            complement(5761..6459)
                     /gene="liaF"
                     /locus_tag="ACL73L_001605"
     CDS             complement(5761..6459)
                     /gene="liaF"
                     /locus_tag="ACL73L_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HGS0772553.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAVTILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILR
                     KMVGTTKIIVPVDVEVSLSVNCLYGDLTFFNQPKRALRNEHYHQETKDYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            complement(6537..7547)
                     /gene="fni"
                     /locus_tag="ACL73L_001606"
     CDS             complement(6537..7547)
                     /gene="fni"
                     /locus_tag="ACL73L_001606"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HGS0772554.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAESCGILFVTGSYSAALKNPTDDS
                     FSVKSSHPNLLLGTNIGLDKPVELGLQTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYSKQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SSQRDYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKAVGLSRT
                     VLELVETYTVEEVIGIVQGWKADLRLIMCSLNCATIADLQKVDYLLYGKLKEANDQMK
                     KA"
     gene            complement(7531..8538)
                     /locus_tag="ACL73L_001607"
     CDS             complement(7531..8538)
                     /locus_tag="ACL73L_001607"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HGS0772555.1"
                     /translation="MIAVKTCGKLYWAGEYAILEPGQLALIKDIPIYMRAEIAFSDSY
                     RIYSDMFDFAVDLRPNPDYSLIQETIALMGDFLAVRGQNLRPFSLKICGKMEREGKKF
                     GLGSSGSVVVLVVKALLALYNLSVDQNLLFKLTSAVLLKRGDNGSMGDLACIVAEDLV
                     LYQSFDRQKAAAWLEEENLATVLERDWGFFISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLSSSKETVVSLVEALEQGKAEKVIEQVEVASKLLEGLSTDIYTPLLRQ
                     LKEASQDLQAVAKSSGAGGGDCGIALSFDAQSTKTLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            complement(8525..9478)
                     /gene="mvaD"
                     /locus_tag="ACL73L_001608"
     CDS             complement(8525..9478)
                     /gene="mvaD"
                     /locus_tag="ACL73L_001608"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HGS0772556.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEVVPATSSISLTLENMYT
                     ETTLSPLPANVTADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YPVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMDFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEHLSEIFGHRYRLIVSKTKDLSQDDCC"
     gene            complement(9460..10338)
                     /gene="mvk"
                     /locus_tag="ACL73L_001609"
     CDS             complement(9460..10338)
                     /gene="mvk"
                     /locus_tag="ACL73L_001609"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HGS0772557.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVP
                     AESPWRLYEEDTLSMAVYASLEYLNITEACIRCEIDSAIPEKRGMGSSAAISIAAIRA
                     VFDYYQADLPHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEGLGQI
                     LSQAHLHLKEIGVSSPEADFLVETTLSHGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
     gene            complement(10460..10789)
                     /locus_tag="ACL73L_001610"
     CDS             complement(10460..10789)
                     /locus_tag="ACL73L_001610"
                     /inference="COORDINATES: protein motif:HMM:NF024736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGS0772558.1"
                     /translation="MTRKVYDTDLNDQEWAKIEPYFSKHRTYKWPKRVLVNETLYVTK
                     TGCQWRMLPHDFPLYLMVWSFFRRSMTTGWFQVNGRWYYAYSSGALAVNTTVDGYSVN
                     YNGEWVQ"
BASE COUNT         3248 a         2510 c         1940 g         3227 t
ORIGIN      
        1 gtctcccaag tcgtacgata cttcttaact ggctagcaca atacaggaaa aacgggtata
       61 ctattgttga gaaaacaaga gggagagtac ctgagagcgg agaatgccat cctaaaaaag
      121 ttaagagaac tccgattgaa ggaggaaaaa gagaaagaag aaagacagaa attattcaag
      181 aattaatgac tgagttttcg ttagatattc ttctaaaagc cattaaacta gctcgttcga
      241 cctactacta tcacttgaaa cagctagaca aaccagataa ggaccaagag cttaaagctg
      301 aaattcaatc catttttatc aaacacaagg gaaattatgc ttatcgccgg gttcatttag
      361 aactaagaaa tcgtggttat ctggtaaatc ataaaagagt tcaaggcttg atgaaagtac
      421 tcaatttaca agctaaaatg cgacagaaac gaaaatattc ttctcataaa ggagacgttg
      481 gcaagaaggc agagaatctc attcaaggcc aatttgaagg ctctaaaaca atggaacagt
      541 gctacacaga tgtgactgaa ttttccattc cagcaagtac tcaaaagctt tacttatcac
      601 cagttttaga tggctttaac agcgaaatta tcgcctataa tctttcaact tcacccaact
      661 tagaacaagt aaaaacaatg ttggaacagg cattcacaga gaagcactat ggtggagtcc
      721 ttctttggga ttctgaaatc ggaaatgttt tatggctatg agaaaacatt taaatcactt
      781 aatcaattgg aacaagccat tatagactat attgattatt acaacaacaa gagaattaag
      841 gtaaaactaa aaggacttag tcctgtgcag tacagaacta aatcctttgg ataaattatt
      901 tgtctaactt ttggggggca gtacagaaaa accttgatac tatgcgtttt ttgattgtga
      961 agacttttat ccacctatta tacaaagtta agtatccatc acagtttaca attagccttc
     1021 attacttaac ccattcacca ttatagttta agtagtaacc acctactgtg gtattaacag
     1081 ctaaagcacc tgaatcatag gcatagtacc agttaccatt gacttggaac cagcctgttt
     1141 tcatatctcc attacttgca tttagatagt accaagtcga accttcctga taccaaccag
     1201 ttgccatagc tcctgatgaa cggagatagt accatttgtt cccaaggttt tgccagccag
     1261 ttctcatatc accattttca gcatctagat agtaccaagt tgagccttcc ttataccagc
     1321 cagttgccat agctcctgac gaatggaggt agtaccactt attacctaga tattgccaac
     1381 ctgtttgcat aatgccagtt gctggatcta ggtagtacca tggagctgct tttagcttct
     1441 cttcatcata ggtaagaggg taatccttaa cccaaccacg tgctagctct cccaccgtca
     1501 atctgttgat gcgcgaatca aagccaccat ccttctgtaa ataataccaa tgaccctctt
     1561 cataaatcca accagttttt aaagtatgat aactacgctg atcttcaaaa taatagactc
     1621 gtttctctgc tgggctatca tattcttccc catgatgttt gttggtattc gtagcagttt
     1681 ttgcttctaa aacttgcttg ccaacaaatt cttgtaatac accatcttga ccaaaataaa
     1741 accaatctgg tctaagagaa atctctattc ttggagaagg accaatcgta acccccttgt
     1801 gtggagcagg tatatattgc cagccgacaa ccatctctcc ggataaagga tcaaaataat
     1861 agtacttacc atcaatcact cgccagtagg tttcttttag atcccccttt ttgtagtagg
     1921 ttctaccatt ttcttggaca aactgccatc ctacagaatc atctgcaaat actgtactgg
     1981 tccctagcaa accgaagaaa aatgtggcta gtacgacttg catcattttt ttcaaaagct
     2041 tcattttata tatcctccaa cattaaatcc actcaccaga tgaagcgaag ttataaactt
     2101 taccatcgat agtttggcta cctgtaacca ttgctctatt tgaattgaga taataccatt
     2161 ttccatttac tttttgccaa tctgtaagca tcttaccgga actgtctaaa taataccaag
     2221 tatcatttat ctcctgccaa cccgtttgtt tatcatgttg tctataatag taccaactac
     2281 catttatttt cttccatcct tcaagagagt aacctttaag aaccgaataa ataaatggca
     2341 aatttcgttc atttaattta actgcactgt taatttgatt agctccatct cctaaagtat
     2401 gcactcctac tacgcggtga ctagcatcat aaactgcaga tccactagac ccctctaaag
     2461 tatcaacttg gtaatccaag aacatgccat catcacttaa aacttgtttt ttatctgtat
     2521 acatttgatg aattttaaaa ttatatgata gatagcctgt gatagtcaca gttattcctg
     2581 tcaaattttt ttgactagta ggaagaccca aagtccctaa ttttgcacca atgggctctt
     2641 ctagaattaa taaagccaag tcatattccc ttgcattctt agaatttaaa tttctaaatt
     2701 ccttcaaata acgaacttcc tttactttga tctttccaaa tagttcttga cttggactaa
     2761 cagccggaag aacataaata tcatccgctt ctttcccata gtcatgtctg tagtagttgt
     2821 gagccgctgt aattaacaca ttatctttaa taaaattcgc acttcccctc ccttctacat
     2881 tcattccatc ggctactcca taatacttag ataaaaccat agctgaagtc gaatacggat
     2941 actgtaaagt attatcaatt ttaatcaaat catcattacc gataatactt ctgattgctt
     3001 ttggtagtat gaaccatacg ttggtgaaat ctcagataat gaagaatcat tagactctgg
     3061 acctttttct agtgtctcac ttacctcata ttcttcacct gtactagaaa taacactcaa
     3121 agcagatact gtcgataact ggctagccaa taaagtactc gcaataattg aaatacccaa
     3181 ttttttataa acagttttct tcattattgt atcctcctaa tgtaattata gcgtactatt
     3241 ctaaatttct taatctacta tagaatcaag aaatctacca ccttctttaa ataccctcca
     3301 ttatcacata aacaggtaaa cttttcaatt aatgactgcg cttttcaatc acgctagagg
     3361 tacttacttg cttctttgat actaagttca gccattcttt ccttgttttt ctcaataaag
     3421 catgttaccc aagtgggatt cgttttggag tagtctcgca gagtccagcc aatggcttta
     3481 ttgataaaaa attctgtttg gttcaagtta tgaagtagaa tcttttccat taattgagta
     3541 ttggtcttct cttttattaa caactggtgg tcaatagcga cacgtctcag ccagatatta
     3601 tctgataggc tccattttat actcaatgaa aatcaaagag caaactagga agctagctgc
     3661 aggttgctca aaacactgtt ttgaggttgc agatagagct gacgtggttt gaagagattt
     3721 tcgaagagta ttaagattat ttcttctagt tcagggtgtt catacaccaa actccctact
     3781 actcgatcta ggatatctac cgtgtcccac caggattttg tcacgactaa ctgctctagc
     3841 ttaggcaaat cggtttcctt tagataagac tgcattgctt tcaaatagtt agcagccaca
     3901 tattggtatt ttctaggctc cttttcccag caagtgtctg caaaatccca atcgataatc
     3961 tttgtttttt tcgcttctgg taaatatttt tatagagttt atttctttca ggcaccgcaa
     4021 tacctagaaa agaaaattga tggcgcatat aggcttccat gggccttgct ttttagagtc
     4081 ttttgctgct tctagctcct aaagtaaatc tgctaaactc atctaaaact cctcatgccc
     4141 caccaaatgg tgctgaaagg cgtagacagc cgcctgggta cgatcgctga cttcaagttt
     4201 ggcaagaatg ttggacacgt gggtcttgac cgtcttgaga gagataaaga ggtcatctgc
     4261 gatgcgctga ttttcgtagc ccttggcgat gagttggaga acatctcgct cacgcgcagt
     4321 caattcttca tgaagttcca tatgattgcg gtggtattca accttcttgc taacctcttg
     4381 ctcaatggcc agctcgccag cagctacctt actgacggca tgaagcaatt catctgcact
     4441 agaagtcttg agcatatagc ctttggcacc agcatctaag actggcatga ttttttcatt
     4501 gtccaaataa gaggtcacaa tcaaaatctt ggcttcaggc cattctttaa ggattgctaa
     4561 ggtcgcgtca attccattca tctcaggcat gacaatatcc atgacaatga catctggacg
     4621 cagttccaag gccaagtcaa tcccttgaga cccgttggac gcctcaccca caacttctac
     4681 atcgtcttgg aggtcaaagt agcttttcaa acccaaacgg accatttcat ggtcatctac
     4741 tagtaaaatt ttcatcttta ctcctttatc attccttatc taacagggga atacggatat
     4801 caactgccag cccttgcttg ggagctgtta ataactgaac cgtccctgcc atatcttcaa
     4861 cccgctcctt gatatttcgc agtccataac tcaagtcgtc taagctccct aactggaaac
     4921 caatcccatt gtccaccacc ttcagttgca attcaacatc tgtctgatag aggtagacat
     4981 ctaggcaaga tgcctgggca tggcggagcg tattgctaat caactcttgc aggatacgga
     5041 agatatgctc ctcgattttc ttaggcaatt tcgtcatatt ctgcttgaga ctaaccctaa
     5101 gatcactctt gtcctcaagc tcttttaaga gaatttgaat cccttctatc aagctcttct
     5161 gctccagttc aactggtcgc aaatgcaaga gcaaaacccg caaatccttc tgggctgttt
     5221 ctaaaatagc tgtgacactc tgcaactggg tctgcatctt ttctctatcc aatttcaaag
     5281 cctgctgact gatacccgat aaaatcatgt gggccgcaaa caactcctga ctgactgtat
     5341 cgtgcaaatc ccgagcaatt cgcttccgtt ccttctcgat gatttcctct tcctgagcaa
     5401 ggctatgatt ttcagctttt tgaagagcct ctgtcaaaag gttaagttta cctgataagg
     5461 acttgaaact ggcatccaaa tcttgatcta caacctgaac tacttcttgc cctgccaata
     5521 aacgcttgag attagcctgc atttttctta gagaaagctc ttcgatccct cgccaaaaca
     5581 gggctaagag acaggtcatg gacatgctga aaaccaacaa taaaaagaca aatttttctg
     5641 ttttttcgac atcgtgcaaa aagatagacc agtcaaaatc aagtatttcc agcaagctgt
     5701 gggagaaaaa aaagacaaat aggaaggagg tgagagcaat aatgacatag gcttgttttt
     5761 tcatcctcta accacctcca catcaccaat catagtggtc aagaaaatct tgacactctt
     5821 gttactcttg agatagtctt ttgtttcttg atgatagtgt tcattgcgga gggctcgctt
     5881 gggctggttg aaaaaagtca aatccccata gagacagtta acgctgagac tgacttccac
     5941 atctacaggt acgatgattt tggtcgttcc taccatcttt ctgaggataa tgacattgtc
     6001 atgattggtt aagatgaccc tctccagatg aatagtgtcc ttgcccatga agcgaaagag
     6061 attgatatca tcgaattggc aagtctggta gcttgaaaaa tgatgaagat ttccaaacca
     6121 acgatttttc tccttcttaa ccgtcacaac ctcttcaaaa accaaattgg tctgctcttt
     6181 ttcctggttc atcatcgggt aaagaagaaa gaggctatag ataaccgcaa caaaaatagc
     6241 tagaatcaca aaaggattga gcataacgat gaaaaagaag agaatggtta ccgctactaa
     6301 aagaagatta tttccctctt taccagtgta gtagcgaatc aaaagcaaaa agaggaatag
     6361 tatcagcaga aaacgcgaaa aatgctctga taccatcaaa atcagagctc ctgtcagaag
     6421 acaggcttcg ataaataaaa agattttaaa ttttctcata ggttcatcct ctcccttcta
     6481 ttttatcaca attcaaaaaa gtcacctcag tctgaggatg gaaaaaaagg cgctggttac
     6541 gcctttttca tctgatcatt tgcttctttt aattttccat aaagaagata gtctactttt
     6601 tgtagatctg ctatggtggc acagttaagg gaacacataa tcaagcgtag atctgctttc
     6661 cagccttgga caatgccaat cacttcttca actgtgtagg tttcaaccaa ttccagaacg
     6721 gttcgtgaca atcccacagc cttagcacca aaaaccaagc acttaatcat atccagcgga
     6781 ttccgaaccc ctccactaac caagagttcg accttatctt tccattcttg ggcattgaga
     6841 agggcctgca tggtagactg accccattga ttgaggtaat cacgctggct actacgacgg
     6901 ttttcgatat aggcaaagct ggtgccacca cgacccgata ggtccactgt acgaacaccg
     6961 aattcatagg ctctttcgat tgtcttggca tccattccaa agcccacttc cttgaggaca
     7021 ataggaacgg gaatttgctt gctataatct gctagatgcg attgccagct tctaaacttc
     7081 ctttctccct cgggcatgag taattcctgc atgacattga catgcacttg caatagcaca
     7141 ggattcatct cttctacagt ctgaagtcct aactcgacag gcttgtccaa tccaatattg
     7201 gttccaagga ggagattggg atgactagac ttgacagaaa aagaatcatc cgttggattt
     7261 ttgagggctg cgctataaga acccgttaca aataaaatac cacaggattc cgccacctga
     7321 gccagctttt gattgatttc tcttccctta ttacttccac cagtcatggc attgatataa
     7381 aaaggaaagt cccactttcg accagcaaac tctgtcgaaa gatcgatttc atccagattg
     7441 taaagaggca aggaagaatg aatcagctcc acctcatcaa agctattata ggaacttttc
     7501 tgctcaaggg catagagaat atgctcgtcc ttacgatttg tcgtcatgtc ctatcctttc
     7561 ttgatataag agctcaatcc ccagatcggc ccaacgattt tttaaggttt tggttgattg
     7621 cgcatcaaaa ctcagggcga tgccacagtc accaccacca gcaccactac tcttggcaac
     7681 ggcctgcaaa tcttgactgg cttctttcaa ctgtctaagc aaaggcgtgt aaatatctgt
     7741 actcaagcct tctaaaagct tgctggctac ttctacttgc tcgataactt tttcggcttt
     7801 cccctgctcc aaggcttcga ccaaagaaac caccgtttct tttgaggaac ttaaaaaatt
     7861 ttgattgata ttttgcttga tttgctggac catgtgactc gatacagcca cttccttggt
     7921 ccatcccact aagaaatcac attctaaagt tggtttcact tgtgagataa aaaatcccca
     7981 atcacgctcc agaactgtcg ccaagttttc ttcttctaac caagcagccg ccttctggcg
     8041 atcaaatgac tggtagagaa ccaaatcctc tgccacaata caggcaaggt cgcccatgga
     8101 accattgtct cctcgcttga gcaagacagc gctagtcagc ttgaacaaga gattctgatc
     8161 aaccgaaaga ttatagagag ccaataaagc cttgacaacc aagacaacga cgctgccact
     8221 agaacctaga ccaaactttt tcccttctcg ttccattttg ccacagattt ttagggaaaa
     8281 aggtcttaaa ttctgaccgc gaacagcgag gaagtctccc atcaaagcaa tcgtttcttg
     8341 aatcaagctg tagtcaggat taggccttaa gtccactgcg aaatcaaaca tatctgaata
     8401 gatacggtag ctgtcagaaa aagcaatctc agccctcata tagatgggaa tatcctttat
     8461 caaagctaac tgccctggct ctaaaatagc atattcacct gcccaataga gttttccgca
     8521 agttttaaca gcaatcatct tgactcaaat cctttgtttt tgacacaatc aagcgataac
     8581 gatgaccgaa aatttctgat aaatgctcca agtctttctc ctgacagaag accttaacat
     8641 tgggaccagc atccatggta aagtagcagg cctctccttt ctcacgaagc tggcgaacaa
     8701 agtccatagc ctcataagag gcatccgtca gataagaaaa ggctggacta gcagtctttg
     8761 tcgtagcatg catagccagg gcatttttct ccgttaattc tccaatcttg gcaaaatcat
     8821 tttccttgag ataaatcagc atatcctgat agtccttctc agactgacga acccagtcgt
     8881 caaaagtcgt cgaggtttcc acacaaagtt tcatcccgtc acggctagag attggttttt
     8941 tcttgtcctc tagcaccaac ataatcatag ctagtttcaa gtctgtctct acagggtaaa
     9001 tttctccact atccttatcc caggctccta gtggtccata aaaactccga gaagaagaac
     9061 ctgaggcaaa tttggcttcc tgtgccaact gacttctatc caatccaagc ttgaaataag
     9121 cattacaagc cttgaccagg gcggacaaac cactagaact tgaggacaga cccgctgccg
     9181 taggcatatt gttttgagta tcgatacgga caaagccctc accagctgga cgataacggt
     9241 caataatctt actcatcttg gcatgctcga cctcattttg tagctgacca ttgatgtaaa
     9301 attcgtcagc tgttacattg gctggtaaag gcgacaaggt cgtctctgta tacatatttt
     9361 ccaaagttag agaaatactg ctagtagcag gcaccacctc tttttctttt ttctttcccc
     9421 aatatttgat aatagcaata tttgcgtagg aacgtactgt tacaggctct ctatccatgt
     9481 ctgaacagct cctttctctt ctaatctttc tgctagttct tgtgcgtgtg tcaaattggt
     9541 taccaaggct atgatacaac ctcctagccc accaccgctc atcttggcac ccagagcacc
     9601 atggctaaga gtcgtttcaa ccaaaaagtc tgcctcaggg ctactgactc caatttcttt
     9661 taaatgtaaa tgcgcttgac tgaggatttg tcccagtcct tcagcatctt tttgtgaaat
     9721 cgcaacttct gcttgctggg ttaattctcc caaggcatgc aaaaacggta gggcatcctt
     9781 gcccttattt tgaaccactt ggatggcttc acgagtatga ccataaacac ccgtatcggc
     9841 aatcaccaaa taggcggata aatccatctc aagttctgta aatcctacgt tcttgataaa
     9901 gcgaataggt tggtcactaa gacaggtctt agcatccaaa ccactaggat tcatatgggc
     9961 aatcatttca gctcgattga ccaagatttc tagtacatca tgaggcagat cagcctgata
    10021 gtagtcaaat actgcacgaa tggccgctat gctgatagcc gctgacgaac ccatcccccg
    10081 tttctcaggg atagccgagt caatctcaca acgaatgcag gcttctgtga tattcaaata
    10141 ctccagtgag gcataaaccg ccatggacaa ggtatcctcc tcataaaggc gccaaggact
    10201 ctctgcagga actaccttac aggtcacctc cacctccaaa agaggcaggg aaatggcagg
    10261 ataaccgtaa acgaccgcat gttcccctat taaaattatc ttactatgtg cctgaccgac
    10321 accaactttt tttgtcattt tttcctttta ctagacgaaa aaacgtctta tttttcatac
    10381 aagtattaat tctttcctat ctattttatt atattttcac aaaaaaagcg attgtttcca
    10441 ttcacaatcg cttctttcat tattgaaccc attcgccatt atagttgaca gaatagccat
    10501 ctacggtcgt attcactgcc aaggcacctg agctataagc gtagtaccat ctgccattga
    10561 cctggaacca acctgtcgtc atagaacgac gaaagaaact ccataccatt aagtaaagag
    10621 gaaagtcgtg agggagcatg cgccattgac aacctgtttt agtgacgtac aaagtctcat
    10681 taacaagtac tcgtttcggc catttatagg tgcggtgttt ggagaaatag ggttcaatct
    10741 tagcccattc ttgatcgttt aaatcagtat catatacttt gcgtgtcata actctagttt
    10801 aacatttttt gtgaatacag gttctaaata atcgaccacg aaaatttctt aagtggaaaa
    10861 cgccttatga agtatgctac gggaaagtta tgcacttaat ttgacaattc aagatgtaaa
    10921 aatat
//
LOCUS       DBERDK010000061        10873 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.061, whole genome shotgun sequence.
ACCESSION   DBERDK010000061 DBERDK010000000
VERSION     DBERDK010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 10873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10873
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.061"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(85..1605)
                     /locus_tag="ACL73L_001611"
     CDS             complement(85..1605)
                     /locus_tag="ACL73L_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HGS0772559.1"
                     /translation="MTNLIATFQDRFSDWLTALSQHLQLSLLTLLLAILLAIPLAVFL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLKGIDPSLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLATLIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALVAL
                     LLGLSYSPALLAQKEKENVIIAGKIGPEPEILANMYKLLIEENTSMTATVKPNFGTTS
                     FLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDGIAKQDHLAYLKP
                     MSYQNTYAVAVPKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVATMQPALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKHPELERVLNTLAGKITESQMSQLNYQVGVEGKSAKQVAKEFLQEQGLLKK"
     gene            complement(1598..2326)
                     /locus_tag="ACL73L_001612"
     CDS             complement(1598..2326)
                     /locus_tag="ACL73L_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGS0772560.1"
                     /translation="MIEYKNVALRYTEKDVLRDVNLQIEDGEFMVLVGPSGSGKTTML
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLSTGYVLQAIALFPNLTVAENIALIPEM
                     KGWSKEEITKKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKIFLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVA
                     NPETILKAPATDFVADLFGGSVHD"
     gene            complement(2341..3105)
                     /locus_tag="ACL73L_001613"
     CDS             complement(2341..3105)
                     /locus_tag="ACL73L_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HGS0772561.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVAEAQAQSVGDTYIQHSTGVPDGK
                     EGFAAFFADFFERHPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFRADENG
                     RIVEHWDVIDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTEIFQNGELEQW
                     SDYVADDLIQHNHDIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     YAQYDIFRLENGKIVEHWDNKEVMPKVEDLTNRGKF"
     gene            complement(3109..3552)
                     /locus_tag="ACL73L_001614"
     CDS             complement(3109..3552)
                     /locus_tag="ACL73L_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HGS0772562.1"
                     /translation="MKDGHLLAHHIRLLNGRIFQKLLSQDPEALYRGEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKKLEEQKLVIVSPCGKDKRKKYLVLTELGKSQ
                     KEVGHRVSQKLDTIFYKGFSEEEIHQFEGFQERILANLKEKGNEV"
     gene            complement(4234..4530)
                     /locus_tag="ACL73L_001615"
     CDS             complement(4234..4530)
                     /locus_tag="ACL73L_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HGS0772563.1"
                     /translation="MAEPNLESLIKDLYNHARHDLSEDLVAALLETTKKLPTTNEQLQ
                     AVRLSGLVNRELLLNPKHPAPELLNLARFIKREEAKYRGTATSALMYEELFKML"
     gene            complement(4552..5616)
                     /gene="pepA"
                     /locus_tag="ACL73L_001616"
     CDS             complement(4552..5616)
                     /gene="pepA"
                     /locus_tag="ACL73L_001616"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HGS0772564.1"
                     /translation="MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFK
                     LLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKAEAESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALVKKLDRSTVDLIKHY"
     gene            6017..6718
                     /locus_tag="ACL73L_001617"
     CDS             6017..6718
                     /locus_tag="ACL73L_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HGS0772565.1"
                     /translation="MTKTILVTGGTSYIGSHTVKALLNAGYQVHVLDNLSTGNRAAVD
                     SHASFKELDVYDASALKAYLEENQIDAVLHCAGEIVVSESIENPSKYFTTNVAGMNQV
                     LKVLSEVGIQKIMFSSTASLCGNNCIDKPVTEDTLLDPVNPYAETKLMGERMIYWMAN
                     RYDWKYVIFRYFNVAGAEMDASNGLRVKNPTHIIPNINKTALGQHDSLKIFGDDYDTR
                     DGSCIRDYIYVLDLA"
     gene            6734..6844
                     /locus_tag="ACL73L_001618"
     CDS             6734..6844
                     /locus_tag="ACL73L_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase"
                     /protein_id="HGS0772566.1"
                     /translation="MNYLFQEDSSSQIFNLGTKKGYTIKEIFKTAEELLN"
     gene            7141..8100
                     /locus_tag="ACL73L_001619"
     CDS             7141..8100
                     /locus_tag="ACL73L_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS0772567.1"
                     /translation="MKLSHYLIGLLLLLVFLSISIGTSDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGMVLSLFVFPSAS
                     LTQKMLFAFVSSIVFTLFFLAFMTIFTVKERWMLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKETSESLGISYS
                     LLEKLALFLVALTTSVTMITVGDLPFLGVIVPNLVRKRYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRPYELSVSLLLGIIGSLVFILLLWRGGRKDAD"
     gene            8090..9046
                     /locus_tag="ACL73L_001620"
     CDS             8090..9046
                     /locus_tag="ACL73L_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HGS0772568.1"
                     /translation="MQTKSKHTKLFWILIILAIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLVAIATGISTISFQTLTENRFLTPSILGIESFYVLLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSLFFLALQGYLKTLMKQDLVFVLLICLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATALVGPMAFFSFMLANLTYLIVKDYQHKLLFIVAILVGF
                     ISLTLGQALIERVFALEIRISMIIESVGGFLFFILLYRRARQ"
     gene            9043..9795
                     /locus_tag="ACL73L_001621"
     CDS             9043..9795
                     /locus_tag="ACL73L_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0772569.1"
                     /translation="MKLENIDKSIQKQDILQGISLEVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIESWNSQELAQELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINMASQYADEIVAFKDGQVFSKGR
                     TDQIMQADLLSQLYEIPITLADINNKKICIYS"
     gene            9891..10856
                     /locus_tag="ACL73L_001622"
     CDS             9891..10856
                     /locus_tag="ACL73L_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HGS0772570.1"
                     /translation="MKTSLKLYFTALVASFLLLLGACSTNSSTSQTETSSSAPTEITI
                     KSSLDEVKLSKVPEKIVTFDLGAADTIRALGFEKNIVGMPTKTVPTYLKDLVGTVKNV
                     GSMKEPDLEAIAALEPDLIIASPRTQKFVDKFKEIAPTVLFQASKDDYWTSTKANIES
                     LASAFGETSTQKAKEELAKLDKSIQEVATKNESSDKKALAILLNEGKMAAFGAKSRFS
                     FLYQTLKFKPTDTKFEDSRHGQEVSFESVKEINPDILFVINRTLAIGGDNSSNDGVLE
                     NALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKALK"
BASE COUNT         3151 a         2543 c         2015 g         3164 t
ORIGIN      
        1 aaagcccaga cgaaattgtc tgagcattct tttatctagt cgtttaagga agttgagttc
       61 agtatgttta aagtctctgt cccatcattt cttcaacaaa ccttgttctt ggagaaactc
      121 cttggctact tgctttgctg acttgccttc aacaccgact tggtagttga gctggctcat
      181 ctggctttct gtaatcttac cagccaatgt attaagaact ctttccaact ctgggtgttt
      241 cttgagaaga gcttctttca tgagtggagc cccttgataa ggtgggaaga gttgcttgtc
      301 atcttccaag acctgtaaat cataacgctc caattccgca tcagtcgaat aggcatccgt
      361 gatttgaata tcccctgact gaattgcctg ataacgaaga gctggctgca tagtcgccac
      421 attgagattg agaccataca ttgattgcaa gcccttattt ccatcttcac ggtcgttaaa
      481 ctcaagcgta aagcctgcct tcagctgccc ttccactttt ttcaaatccg aaatggtctt
      541 caagccatat tcttgagcaa tctttttcgg aacagctaca gcataggtat tttgataaga
      601 cataggtttg agataggcta gatgatcctg cttggcaatg ccatcacgcg ccacttgata
      661 aacctgctct ggctcatgac tcaccttggg tgatggttga agcaaacttt cagtcaccgt
      721 accggtaaat tcaggataaa tgtcaatatc accttttttc agagcttcat aaaggaaact
      781 tgtcgtccca aaattcggtt taacagtcgc agtcatgctg gtattttctt caatcagcaa
      841 tttatacata ttagccaaaa tttctggttc tgggcctatt ttcccagcaa taatcacgtt
      901 ttccttctct ttttgagcca aaagggctgg actataagac agacccagta ataaagccac
      961 caaggcaaaa ccagagaaaa tcgtccgcaa tttcgctttt tccattactt ttagtaggaa
     1021 gttaaaggca atagctagca ctgcagaaga aagggcccca atcaaaatca aactggcatt
     1081 attacggtca attcccaaca ggataaagga acccagtccc cctgccccaa tcaaggtcgc
     1141 caaggtagcc gtaccgataa tcaagaccgc tgccgtccga attccagaca tgataacagg
     1201 catggcaagt ggaatttcaa acttcttgag acgctcccat ctggtcatcc caaaggcaat
     1261 cccagcctct tgaagactcg gatcaattcc cttcagccca gtaatagtat tttgtaaaat
     1321 cggaaaaatc gcataaatca ctagagctgt caatgccggt aaagtcccaa ttcccatcaa
     1381 gggaataaag agtcccaaca aggccagaga cgggatggtc tggaaaatac ctgcaatctg
     1441 caagacccag tcggccagct tctcatgata gcgaagaaaa acagccaagg gaatcgcaag
     1501 caaaatagct agtaacaagg tcaaaagtga caactgcaaa tgttgagata gagctgtcaa
     1561 ccaatcacta aaacgatcct gaaaagttgc aattaaatta gtcatgaaca ctacctccaa
     1621 acaagtctgc tacaaagtct gttgcaggcg cttttaaaat tgtctcggga ttcgctacct
     1681 ggcgaatttc tccatcctgc aagacagcaa tacggtccgc caacttcaag gcttcatccg
     1741 tatcatgggt tacaaaaatc gttgtcatcc caaactcttt atgcaattct tttgtcagaa
     1801 cctgcaactg ttttctcgaa atagcatcca aggccgaaaa gggttcatcc atgaggaaaa
     1861 tcttgggctg accaatcata gctcggacaa taccgacccg ttgctgttct ccaccagata
     1921 attcactagg taagcgatgc ccatactcgg ctactggtaa accaacctta gccaaaagct
     1981 cttctgtttt cttcgtaatt tcttccttgc tccacccctt catttcagga atgagagcaa
     2041 tattttccgc aactgttaga tttggaaaaa gagcaatagc ctgtaaaaca taaccagtag
     2101 aaagacgaag ttcacgctca tcatagtctt tgatgcgctt cccatccata taaatatttc
     2161 catcagttgg ttccaaaaga cggttaatca tcttgagcat ggtcgtctta cctgacccag
     2221 aaggccctac taaaaccata aattccccat cctcaatctg taagttgaca tctctcaaga
     2281 catccttttc tgtgtagcgc agtgctacat ttttgtattc aatcattctt tgtcctcaat
     2341 ttaaaacttc cctcgattgg tcaagtcttc taccttaggc atgacttcct tattatccca
     2401 atgctccaca attttcccgt tctctaaacg gaagatatcg tactgggcat aagcaacgcc
     2461 atcaatctga gtctgaccat agctaaccac atagtttcct tgtcctaaga gttggaaaac
     2521 aaagtcaaaa gtgacactat attcagccac atagttttta taagcagcac ttccttgtcc
     2581 aatatcatga ttatgctgaa tcaaatcgtc tgccacataa tcactccact gctctagctc
     2641 cccattttgg aaaatttctg tcaagaaacg gcgaaccagc tttttatttt ctgctttctt
     2701 gtccaaatcc ttgatttcaa aatctccaaa aatttgatct agttggtcat tttcaggtgt
     2761 tcgatagtag tcaatgacat cccaatgctc aacaatacga ccattctcat ccgcacggaa
     2821 agtatccgtc gtcacccatt gagcttctcc accattcaga tattgatgaa catgaacaaa
     2881 gaccagattg ccatcctcaa tggtgcggac aatcttaatc tgacgctctg gatgacgctc
     2941 aaagaaatct gcaaagaagg ctgcaaatcc ttctttcccg tcaggaacac ctgtcgaatg
     3001 ttggatatag gtatccccta cagactgggc ttgagcctca gcaactcgtc cgtcttgaat
     3061 ggcatggatg tataggttgt gagcattttt cacttgttgt gacatattct aaacctcatt
     3121 tcccttctct ttcagattcg ccaaaattct ttcttgaaaa ccttcaaatt ggtgaatttc
     3181 ttcctctgaa aatcctttgt aaaagatagt atccaatttc tgactgacac gatgccccac
     3241 ttctttctgg gacttgccta actccgttaa aactaaatac ttcttacgct tgtcttttcc
     3301 acacggacta acaattacaa gcttttgttc ctctagcttt tttatcatag tcgtcagcgt
     3361 attattcgca agtccagtcg caagcgcgat atctgtcgca gttgcgcagc cagtttcact
     3421 attccataaa accgctaaaa tcttgccctg ttcaccccta taaagagcct caggatcttg
     3481 actcagtaac ttttgaaaaa tccgcccatt caacaaacga atatgatggg ctagcaaatg
     3541 accatctttc ataacacctc caatttattt cgatatcgaa atgaataaaa caattgtaac
     3601 actcatcgtt ctaactgtca actatttcga tttagaaata atttttgata attatccaca
     3661 ccaccatact ccggctcaac taacttttaa cgagagtttc taaactcctt cgtcctccag
     3721 tctacaaaag ccttccattc gtactatcct atattttatg aggggacaca tttttcctat
     3781 cagaccattt attttaaaga tagaagtaaa tcataattgc ttccatctgt tcttttatag
     3841 tatattgaag ttagactaga gcactgtatc ttctaaaaca ttgatagaaa gcgatttgaa
     3901 tttcccaatc aatttgttcg tatttatagc atttcgaaac tggaatagga caccatgact
     3961 gctaaaagat ttctataaat tcatttaatt tcctcaatca atttgttcat atcttatttc
     4021 attccgctat aatttcacct taccctatct ttttcgtagc acccttcaaa cagcctatcc
     4081 cctaccgttt gacgattcct cacttcgctc cacttccatt acagaagttt cttcactact
     4141 atgggctcgg ctgacttctc atgattcctt gttactacta tttgaacgct cacgagatag
     4201 atcttacaaa aaatgctttg atccacaatg gaatcaaagc attttaaaga gttcctcata
     4261 cataagcgca gaagtcgcag ttcctctgta cttggcttct tctcttttga taaagcgagc
     4321 caagttgagc aactcaggtg ctggatgttt gggatttagg agcaattcac gattgaccag
     4381 gcctgagaga cgaactgcct gcaattgctc atttgtagta ggtagttttt tagtagtctc
     4441 taggagagca gcaactaaat cttcactcaa atcatgtcga gcatgattgt aaagatcttt
     4501 tataaggctt tctaggtttg gttctgccat ccctaccacc tcccttatgg tttaataatg
     4561 tttaatcaaa tcaaccgttg aacgatccaa tttcttcacc aaggcttgta agaaagcttg
     4621 cgcttctagg aagtcatcca ttgcatagag ggtttggtga gaatggatat aacgagcgca
     4681 gacaccgata gttgttgatg ggacaccacc atttttcaga tgagctgcac ctgcatctgt
     4741 tccgccttta ccacagtagt attggtactt gataccagct tcttcagccg ttgtcaaaag
     4801 gaaatccttc atccctggga gaagcaagtg acctggatca tagaaacgaa tcaaggttcc
     4861 atctccaatc ttgccttgac caccgtagac atcacctgct ggtgagcaat caactgcgag
     4921 gaagacttct gggtcaaact tggttgtaga ggtatgagcg ccacgcagac caacttcttc
     4981 ttggacgtta gaacccagat agagttcatt gccgagtttt tgacctgata aagcttcagc
     5041 tagctcgctt accatgagga caccgtagcg gttatcccaa gcttttgaga tgatattttt
     5101 ttcattggct gtcaaaattg cagaactatc tggtacgatg gtatcaccag gacggatgcc
     5161 aaaactttct gcctcagcct tgtccgcaaa accaccatca aaaacgatat cggcaatggc
     5221 tggcatggtt ggtcccccct ttccacgagt caaatgcgga ggaacagaac ctgaaatcac
     5281 aggaatttca tgaccatcac gagtcaagag tttgaaacgt tggctgctaa ccaccatggg
     5341 gttccagcca ccgatttcta cgacacggaa ggtaccatct ggcttgattt cgctgaccat
     5401 aaaaccaact tcgtccatat gagaagcgac caagacgcgc ggtgcatcca cagcttctga
     5461 atgtttgata ccaaaaatac cacccaagcc atctgtcacc acttcatcca catgcggtgt
     5521 caacttttca cgaagataag cacggacagg cgcttcatga cctgagactg cagcaagttc
     5581 tgttacttct ttaatttttg aaaataatgt tgtcatttca gttccttctt tctttcatcc
     5641 attttaccac tttttatagg agaaggatag tgggaaggtg gatttctaag ttagtatctt
     5701 agtcctgctc tatcttagaa aaggatagta ttctcttgca tgtagtgcaa aatctagtaa
     5761 acattccaaa attaactcga atatttattt ccaaacaaaa aaacaataca ccatcaaagt
     5821 tgtttggatt tttcatgaaa tttacagaaa atagttgact tccctttctt ctttctttaa
     5881 atatatagtt ggttgagttt ggaatagtac gctgtagctg ctgaaacatt tctagaaatt
     5941 aatttgactt tcctaataga gttgttcata tcttatttca atttactata gtacaaaact
     6001 agaaaaggaa aaaatcatga ccaaaacaat tcttgttaca ggtgggacta gctacattgg
     6061 ctcccatacc gttaaagctc ttttaaatgc tggctatcag gtgcatgtcc tcgataatct
     6121 ctccacagga aatcgagcgg ctgtggatag tcatgctagc tttaaagaac tggatgttta
     6181 tgatgctagc gccttaaagg cttacttaga agaaaatcag attgatgctg tcctccattg
     6241 tgcaggtgaa attgttgtga gcgaaagtat tgaaaatcca agtaaatact tcactaccaa
     6301 cgttgctggt atgaaccaag ttctcaaagt cttatctgaa gttggcattc aaaaaatcat
     6361 gttctcttcg actgcttctc tctgtggaaa taactgtatt gacaagccgg tgactgaaga
     6421 taccctgctc gaccctgtca acccttatgc agagacaaaa ctgatgggcg aacgaatgat
     6481 ttactggatg gccaatcgct acgactggaa atatgttatt ttccgttact ttaatgttgc
     6541 tggggctgaa atggatgctt caaacggtct gcgtgtgaaa aatccaactc acatcattcc
     6601 aaatatcaac aagaccgcat tgggacaaca tgatagctta aaaatatttg gagatgacta
     6661 cgatacacgt gacggttcat gtattcgaga ttacatttat gtcttggacc ttgcataggc
     6721 tcatgttaaa ggaatgaact acctatttca agaagacagt tcttctcaaa tctttaactt
     6781 aggaactaaa aaaggctata ctatcaaaga aatctttaaa actgctgaag aattactaaa
     6841 ttaaaaaatc ccacacgaga ttgttgctcg acgtgctagt gacccagcca gtgtcctagc
     6901 agatgcttca aaagctaaac aatatcttga ttggacggct agctactccc tcgaagatat
     6961 tattttatca gattaaggta aaaacatttt agaataggat aacaaaggag aaagtcttgt
     7021 tgctctctcc ttgttgtatt tattaaattg tcagaaattt tattgacaga ttagaaaaat
     7081 aatgttacat ttatatccgc agatatcttt cgataccaaa tctacatgaa aggacggggt
     7141 atgaaacttt ctcattattt aattggctta cttctactcc tagtctttct ctctattagc
     7201 attgggacca gtgatttttc atggggaaag ctatttgatt tcgaccagca gacctggctc
     7261 ctctttcaag agtcccgtct cccaagaact atcagtattc tcctgactgc ctctagtatg
     7321 agtatggcag gccttctcat gcagactatt acccaaaatc agtttgctgc accgagtaca
     7381 gttggaacga ctgaagccgc caaactggga atggtgctga gcctttttgt ctttccatcg
     7441 gctagtctga cccaaaagat gctcttcgct tttgtttcat ccatcgtatt caccctcttc
     7501 ttcctagcct ttatgaccat ttttactgta aaggaaaggt ggatgttgcc tctgattggg
     7561 atcatctata gcgggattat cggctcagtc acagaagtta tcgcctatcg tttcaatctg
     7621 gttcagagta tgactgcctg gacccagggc tccttctcca tgattcagac ccatcagtat
     7681 gagtggctct tcttaggcct catcatcctg ataaccgttt ggaaattatc ccaaaccttc
     7741 accatcatga atctagggaa agaaaccagc gagagtttgg ggatttccta ctccctactt
     7801 gaaaaactgg ccctctttct ggtggcgcta acgacaagcg tcaccatgat taccgtgggt
     7861 gacctaccat ttctcggagt tatcgttccc aatcttgttc gcaagcgcta tggagataat
     7921 ctaagtcaaa ccaaactcat ggtcgcactg gttggtgcca atctagttct ggcttgcgat
     7981 atcctatccc gagttctgat taggccctat gagttgtctg tcagtctctt gctaggaatc
     8041 atcggtagtc tcgtctttat cctacttctc tggagagggg gacgaaaaga tgcagactaa
     8101 aagcaaacat accaagctct tctggattct cattattctt gccatcggag cttgtcttct
     8161 ctacttttgg cccatcactc acttgtcagc ctttgcttgg aagttgcgtt cccaaaagat
     8221 catcgtttat ctcttggtag ccatcgcgac tgggatttcg accattagtt ttcaaaccct
     8281 gacggaaaat cgcttcctga cgcctagtat tttgggaatt gaatccttct acgtcctact
     8341 acaaacccta ctactggttt ttgaaagcaa gtttcttcaa cttggcaaat cccctatctt
     8401 agaattccta gtcttacttc ttgtccagtc cctcttcttt ctcgccttac aaggttactt
     8461 gaagacactg atgaagcaag acctggtctt cgtcctgctg atctgtctag cgctcagaag
     8521 tctctttcga aatatcagca ccttccttca agtcctaatg gatccaaacg aatacgataa
     8581 actgcaaaat agtctttttg cctcctttca acatctcaac acttccatcc tagccatcgg
     8641 ttctctgatc atcctcgctt tgacaatctt tttctttcga aaagcagtcg ttctagatgt
     8701 cttgcacctg caaagagaaa cggctcagat attgggactc gatgttgaaa aagaacagaa
     8761 agagttactc tggggaatcg tgcttttgac ctcaacggcc actgccttgg taggacctat
     8821 ggccttcttc agctttatgc tggccaacct cacctacctg attgtcaaag actatcagca
     8881 caagttactc tttatagtgg ccattctggt tggatttatt agcttaacct tggggcaagc
     8941 cttaattgaa cgagtctttg cactggaaat tcgtatcagt atgatcattg agagtgtggg
     9001 tggcttctta ttctttatct tactatatag gagggctcgt cagtgaaact ggaaaacatt
     9061 gacaaatcca ttcaaaaaca ggatattttg caaggcattt cgcttgaagt cagtcctcaa
     9121 aaactgacag cctttattgg tccaaatggt gctggaaaat cgactctcct ctccatcatg
     9181 agcagactaa ccaagaaaga tcagggagtt ctcagtatca aaggacgtga aatcgagagc
     9241 tggaattcgc aagaactggc tcaagaacta accatcctaa aacagaaaat caattaccaa
     9301 gccaaattga ctgttgaaga actggtcagt tttggacgtt ttccctacag ccgaggtcga
     9361 cttagatcag aagactggga aaaaatccga gaaactctga actatttgga actgaccaac
     9421 ttaaaagacc gctacatcaa tagcctgtca ggggggcaac tccagcgcgt ctttatcgct
     9481 atggtactgg cccaggatac ggactttatc ttgctggacg aaccactcaa caatctcgat
     9541 atcaagcaaa gcgtcagcat gatgcagatt cttcgacgac tggtggagga actcggcaag
     9601 accattatca tcgtcctcca cgatatcaac atggccagtc agtatgcaga tgaaattgtc
     9661 gccttcaagg acggccaggt ctttagcaag ggaagaaccg atcaaatcat gcaggctgac
     9721 ctgctcagtc aactttatga gattcccatc acgctagctg atatcaataa caaaaagatc
     9781 tgtatctata gctagtaaca taaaagctca agttagagaa ccttcagtct cttagtcaat
     9841 aagattaaga gactccctaa atcgttatca cattttaaaa aggagaaatt atgaaaacat
     9901 cccttaaact ttatttcact gccctagtgg ccagcttctt gctcctactt ggtgcatgta
     9961 gtacaaactc aagcactagt cagacagaga ccagtagctc tgctccaaca gagataacca
    10021 ttaaaagttc actggacgag gtcaaacttt ccaaagttcc tgaaaagatt gtgacctttg
    10081 acctcggcgc tgcggatact attcgcgctt taggatttga aaaaaatatc gtcggaatgc
    10141 ctacaaaaac tgttccgact tatctaaaag acctagtggg aactgtcaaa aatgttggtt
    10201 ctatgaaaga acctgattta gaagctatcg ccgcccttga gcctgatttg attatcgctt
    10261 caccacgtac acaaaaattc gtagacaaat tcaaagaaat cgccccaacc gttctcttcc
    10321 aagcaagcaa ggatgactac tggacttcta ccaaggctaa tatcgaatcc ttagcaagtg
    10381 ccttcggcga aactagtaca caaaaagcca aggaagaatt ggccaagcta gacaagagca
    10441 tccaagaagt cgctactaaa aatgaaagct ctgacaaaaa agcccttgcg atcctcctta
    10501 atgaaggaaa aatggcagcc tttggtgcca aatctcgttt ctctttcttg taccaaacct
    10561 tgaaattcaa accaactgat acaaaatttg aagactcacg ccacggacaa gaagtcagct
    10621 ttgaaagtgt caaagaaatc aaccctgaca tcctctttgt catcaaccgt acccttgcca
    10681 tcggtgggga caactctagc aacgacggtg tcctagaaaa tgcccttatc gctgaaacac
    10741 ctgctgctaa aaatggtaag attatccaac taacaccaga cctctggtat ctaagcggag
    10801 gcggacttga atcaacaaaa ctcatgattg aagacataca aaaagctttg aaataagcca
    10861 cttcaaactc aat
//
LOCUS       DBERDK010000062        10782 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.062, whole genome shotgun sequence.
ACCESSION   DBERDK010000062 DBERDK010000000
VERSION     DBERDK010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 10782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10782
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.062"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(1..705)
                     /locus_tag="ACL73L_001623"
                     /pseudo
     CDS             complement(1..705)
                     /locus_tag="ACL73L_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(801..1781)
                     /gene="dusB"
                     /locus_tag="ACL73L_001624"
     CDS             complement(801..1781)
                     /gene="dusB"
                     /locus_tag="ACL73L_001624"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HGS0772571.1"
                     /translation="MTNLNTPFMIGNVEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLIN
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEALLQLEKA"
     gene            complement(1768..2640)
                     /gene="hslO"
                     /locus_tag="ACL73L_001625"
     CDS             complement(1768..2640)
                     /gene="hslO"
                     /locus_tag="ACL73L_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HGS0772572.1"
                     /translation="MDKIIKTISESGAFRAFVLDSTETVRTAQEKHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGAIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPFVGNGQFLVITDYGTGNPYNSITPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKVAGGFLVQVLPGAKKEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALASLPSSDLQEMKEEDHGAEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            2805..4232
                     /locus_tag="ACL73L_001626"
     CDS             2805..4232
                     /locus_tag="ACL73L_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGS0772573.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKALDSGLELTIHSEDENLRLSIGAATKGRDIRSLFLESAVKYQILVYLLY
                     HQQFLAHQLAQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIRYFYFCLF
                     RKVWSSQEWEGHMQKPERKQEIANLEEICGASLSVGQKLDLVLWAHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKVPSFFAGQHIPLGVEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLTQLIQEMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFKETAEEIFHALPAFQQGTDLDKKILWEWLQLIEYMAENG
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYDLLVTNNPIHKKE
                     QTPVYYLKNDLDMEDLAGIRQLLFT"
     gene            4391..5899
                     /gene="glpK"
                     /locus_tag="ACL73L_001627"
     CDS             4391..5899
                     /gene="glpK"
                     /locus_tag="ACL73L_001627"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HGS0772574.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEV
                     RSNSEIYGKTAPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDGLRMVENSPESEKYA
                     RDSHNNDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDI
                     IDTMQVDTQTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     SVGYWKDLDELKLLNETGELFEPSMNESRKEQLYKGWKKAVKATQVFAEIDD"
     gene            6046..7872
                     /gene="glpO"
                     /locus_tag="ACL73L_001628"
     CDS             6046..7872
                     /gene="glpO"
                     /locus_tag="ACL73L_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HGS0772575.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKSDP
                     MLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTPTANKVLSKDQVLERQPNLKKEGL
                     VGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLT
                     DQVFEIKARLVINTTGPWSDKVRNLSNKGTQFSQMRPTKGVHLVVDSSKIKVSQPVYF
                     DTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNI
                     TIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVE
                     SAVSKLESSTSEKYLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMAEGAMERVVDI
                     LKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQLGVSRGLDSKEAHYLANLYG
                     SNAPKVFALAHSLEQAPGLSLADTLSLHYAMRNELALSPVDFLLRRTNHMLFMRDSLD
                     SIVEPVLDEMGRFYDWTEEEKATYRADVEAALANNDLAELKN"
     gene            7942..8646
                     /locus_tag="ACL73L_001629"
     CDS             7942..8646
                     /locus_tag="ACL73L_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HGS0772576.1"
                     /translation="MMNELFGEFLGTLILILLGNGVVAGVVLPKTKSNSSGWIVITMG
                     WGIAVAVAVFVSGKLSPAHLNPAVTVGVALKGDLPWASVLPYILAQFAGAMLGQILVW
                     LQFKPHYEAEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
                     IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPIPNKGDGDWSYAWIPVV
                     GPVIGAALAVLVFSLF"
     gene            8943..9146
                     /locus_tag="ACL73L_001630"
     CDS             8943..9146
                     /locus_tag="ACL73L_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772577.1"
                     /translation="MPKWSNPDYVNELDPKIVDMLVEFHKSQGTLETPEAQAEIAQKR
                     EEIEQRRAELEDKKQELLNRLNK"
     gene            complement(9233..9841)
                     /locus_tag="ACL73L_001631"
     CDS             complement(9233..9841)
                     /locus_tag="ACL73L_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772578.1"
                     /translation="MSSISPSLVVFADDSLSANSKVVSGEAQFENGSSVRFGDTQVNI
                     LSDEVLEVVNPDGSVDTIERRADGVYINGAFYMAYQKNEIDLNISFRSYDPNVWNYVN
                     TIHGNKQANTFANFMTGAGISYMIGRIGALLGGPWGAIIGGAYFGIQAYQSYLDSQSP
                     YPYYITSTYIHVAQRKWKFITEYYRNSNYTGYVKTVTTYVNF"
     gene            9973..10164
                     /locus_tag="ACL73L_001632"
     CDS             9973..10164
                     /locus_tag="ACL73L_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000930580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772579.1"
                     /translation="MLNAKIFYIFLCLIVIETTGLLLLKRGLEMGYLDTEAIILAVFS
                     FAFYNLCSFAWVCSTIKNK"
BASE COUNT         3081 a         2129 c         2424 g         3148 t
ORIGIN      
        1 cacgatttct tagttctaaa tgaacccggc gataagcata atttcccttg tgttcgataa
       61 aaatggattg aatttcagct ttaagctctt ggtccttatc tgttttgtct agctgtttca
      121 agtgatagta gtaggtccaa cgagctagtt taatggcttt tagaagaata tctaacgaaa
      181 actcagtcat taattcttga acaatttctg tctttcttct ttctcttttt cctccttcca
      241 tcggagttct cttaactttt ttaggatagc attctccgct ctcaggtact cattttctgc
      301 ttgaagacgt tctaattctg tcctctcttc aggtctcttt tgtggcttac gtcccatttt
      361 aggtactctc cctcttgttt tctcaacaat agtatacccg tttttcttgt attgtgctag
      421 ccaattaaga agtatcgtac gacttgggag gccgtattca agagaaactc tatctttagt
      481 tcagccttca tgtaagactt tattaatcat ttcttgtttt aaatcaggaa aatagtaacg
      541 attttttcct tttttgacga actctattcc gtaacgatca atcaatttaa tcatgtacct
      601 aatattagaa ttatttatcc caaatttatt tgaaagcttc tctaagctat atccttgttt
      661 tctaagttca tagatctgaa ctttatcatc ataagttagt ttcataataa aaacacccca
      721 aaagttagat tttttctgtc taactttttt ggggtagttc attttgagct ttgccgtttt
      781 tctgagttgt agatgtaaga ctatgccttc tccaattgca agagggcttc aatctctgct
      841 agggtgctag cttgcgaaat ggctccacgg agtttggcag cgccagatgt tccacggaga
      901 tagtgaggag cgaggccgcg gaattcacga actgcgacgt tttctccttt gaggttaatc
      961 aatcgtttca agtgttcgta ggcgatcttc atcttgtctt caaaggtcaa atcaggtagg
     1021 atttctcctg tttcaaagta atggttgatt tggttgaaga ggtaaggatt tcccatggca
     1081 gctcggccaa tcatgactgc gtcagcacca acttcttcga tgcgttgctt ggcttcttgg
     1141 acagtacgga tatcaccgtt ggcgatgaat ggaatcttgg ttagagcttg ggcaaccttg
     1201 taaagggtct caaggtctgc gtggccagta tacatttgtt cacgggtacg gccatgcatg
     1261 gcgagggcag aaacacctgc agcctcagca gcgagagcat tttctactgc cagagatggg
     1321 tccgcccagc cggtacgcat tttgacagta agtgggatat caaggacaga ctggaccttg
     1381 ttgatgatag agtagatctt gtcaggatcc ttgagccaca tagcaccagc ttcgttcttc
     1441 acgattttgt tgacagggca gcccatgttg atatcgacga tatcggtctt ggtgttttct
     1501 tggatgaatt ctgctgcgcg tgctaggctg tcttcatcgc taccaaaaag ttggatagag
     1561 acagggtttt cgccctcatc gatatgaagc atgtgcaggg ttttttcgtt gttgtattgg
     1621 attcccttgt cagagaccat ttccattaca acgagtccag ctccgagctc ttttgcgatg
     1681 gtacgaaagg ctgagttggt cacgccagcc ataggcgcta aaacggtacg attgggaatc
     1741 tcaacattgc caatcataaa aggtgtatta agatttgtca cgaatgagtt cctccaggtc
     1801 cttttcatca aagttgtaag tagtttggca gaattgacaa gtgatttctg ccccgtggtc
     1861 ttcctctttc atttcctgta agtctgagct tggaaggctg gcaagagcgt tcataaagcg
     1921 ttcatggcta cagtcacatt ggaaacggat ttcttcttca gaaagacgct tgtaggcttc
     1981 gtccccgtag atagccttga ggagggcttc gatatggtcg tcgctttcga gaagagtaga
     2041 gatagctggc atttcttgga tgcgtttttc aaagcgagca atctcttctt tcttggctcc
     2101 tggcaagact tgaactagga aaccacctgc aaccttgacc ttgtcttcct cgtccaaaag
     2161 gacattgagg ccgaccgctg aaggcgtttg ttggctttca gtaaggtaaa aggcaaggtc
     2221 ttcaccgatt tctccagaga tgaggggagt tatagagttg taaggatttc cagtaccgta
     2281 gtctgtgata acgaggaatt gaccatttcc aacaaaaggt ccgactagga cttcaccagt
     2341 cgcagtcttt ttgatgtcaa caccaggatt ttgaacgtag cctttgacgt tccccttggt
     2401 atcagcgacg gtgataatag cacctagaga gctagatccc aacaccttaa ctgtaagttt
     2461 ggtatttcct ttttcattgg ctgcgagaat ctggctagcg ataagagttc gaccaagcgc
     2521 tacagttgag ctagcttggg tttggtgttt ttcttgagca gtgcggacgg tttccgtgct
     2581 gtcaaggaca aaagcacgaa aggctccgct ttctgatata gttttaataa ttttatccat
     2641 agctactatt ttagcataaa aatgcccaaa gggggagccg tgtgtttact gattttcagg
     2701 ataatggacc aggaaatcag catgaaaata aaaagagaaa cagattattt tagcatttgt
     2761 aagatttatg ctatgcttaa ggtagaaaat gaaaggggta acaaatgtat ttaggagatt
     2821 tgatggagaa agccgagtgt ggtcaatttt caatcctttc ctttctatta caagagtctc
     2881 agacgaccgt caaggctgta atggaagaaa caggattttc aaaagcaacc ctaaccaaat
     2941 atgtcaccct gctcaatgac aaggctttgg atagtggctt agaactgact attcactcag
     3001 aagatgaaaa tctgcgtctg tctatcggtg cagctaccaa ggggagagat attcggagct
     3061 tgtttttgga gagtgctgtt aaataccaga ttttggttta tcttctctac caccaacagt
     3121 ttttagccca tcagctggct caagaattgg tgattagcga ggctacgctt ggtcgtcact
     3181 tggctggttt aaatcagatt ttgtcagaat ttgatttatc catccaaaat ggacgttggc
     3241 gaggtccaga gcatcagatt cgctatttct atttctgtct tttccgaaag gtctggtcga
     3301 gtcaggaatg ggaaggtcac atgcagaaac cagagagaaa acaggagatt gccaatttag
     3361 aggaaatctg cggtgcaagt ttgtctgtgg ggcagaaatt ggacttggtt ctctgggctc
     3421 acatcagtca acaacgtctt cgggtcaatg cttgtcagtt tcaagtcata gaagagaaaa
     3481 tgcgagggta ttttgacaat attttctacc ttcgtttgct gagaaaggtt ccgtcctttt
     3541 ttgctgggca acatattcca ctaggagttg aggatggtga gatgatgata ttcttctctt
     3601 ttctcctatc tcatcgcatt cttcctcttc atactatgga gtatattctt ggttttggag
     3661 ggcagttggc agatttactg acgcaattga ttcaagaaat gaagaaggag gaactattgg
     3721 gggattatac agaggaccat gtcacctatg aactcagtca gctttgtgct caagtctatc
     3781 tctataaggg ctatatttta caggatcgct acaagtacca gttagagaat cgtcatccat
     3841 atttactgat ggaacatgat tttaaagaga cagcagagga gatttttcat gctctacctg
     3901 cttttcaaca ggggacagat ttagataaga agattctctg ggaatggctc cagttaatcg
     3961 aatatatggc tgaaaacggt ggccagcata tgcggattgg tctggatttg acatctggtt
     4021 ttcttgtttt ttcaaggatg gcagccattt tgaaacggta tttggaatac aatcgtttta
     4081 ttaccattga agcttatgac cctagtcggc attatgattt gctggttacc aataacccga
     4141 ttcataagaa ggaacagacc ccagtctatt atttaaaaaa tgacttggat atggaagatt
     4201 tggcagggat tcgtcagtta ttattcactt aaaaggcttg gttaatccag gtcttttttg
     4261 tgaaattcac acaatctcct cacatttttt taaaaattaa aaaaagttga taaacaagaa
     4321 agcgctttat tttgtatact agtaagtgca aagaggaaat acctcaagat ctttatcagg
     4381 aggacagtac atgtcacaag aaaaatacat catggccatt gaccagggaa ctacaagttc
     4441 tcgtgccatc attttcaaca aaaaagggga aaaggttagc tcgagtcaaa aagagtttac
     4501 ccagattttc cctcaggcag gttgggttga gcacaatgcc aatgaaattt ggaactctgt
     4561 tcagtcagtt attgcgggtg ctttcatcga aagtggtgtc aagccaaatc aaatcgaggc
     4621 aatcgggatt accaaccaac gtgaaacaac ggttgtctgg gataagaaaa caggacttcc
     4681 tatctacaat gctatcgttt ggcagtcacg ccagacagca cctttggctg agcaactaaa
     4741 aagccaaggt tatgtggaaa aattccatga aaagactggt ttgattattg atgcttactt
     4801 ctctgctacc aaggttcgtt ggattttgga tcatgtagaa ggtgctcaag agcgagcaga
     4861 aaaaggggaa ttgctctttg gtactatcga tacttggttg gtttggaaat tgactgacgg
     4921 tgcggctcac gtgactgact actcaaatgc agctcgtacc atgctttata acattaaaga
     4981 actcaaatgg gatgatgaga ttttggaaat ccttaacatt ccgaaggcta tacttccaga
     5041 agttcgttct aactccgaaa tctacggcaa gacagctcca ttccatttct acggtggaga
     5101 ggtgccaatc tcaggtatgg ctggggacca acaagcagcc ctctttggac agttggcttt
     5161 tgagccaggt atggttaaga atacttatgg aacaggctct ttcatcatca tgaatactgg
     5221 ggaagagatg cagttgtctg aaaacaacct cttgacaacc attggttacg gaatcaacgg
     5281 taaggtttat tatgccttgg aaggttctat cttcatcgca ggaagtgcta ttcagtggct
     5341 tcgtgacggt cttcgcatgg ttgaaaattc accagaatct gaaaaatacg ctcgtgattc
     5401 tcacaacaac gatgaagttt atgtcgttcc agcctttaca ggtctaggcg ctccatactg
     5461 gaaccaaaat gctcgtggtt ccgtctttgg tttgactcgt ggaacaagca aagaagactt
     5521 tatcaaggcg actttgcaat ctattgctta tcaagtgcgt gatatcatcg acaccatgca
     5581 agtggatact cagaccgcca ttcaagtact gaaggtggat ggtggtgcag ccatgaacaa
     5641 cttcctcatg cagttccagg cggatatttt aggcattgac attgcacgtg ctaaaaacct
     5701 ggaaacaaca gctctaggag cggccttcct agcaggtttg tcagtagggt actggaaaga
     5761 cttggacgag ttgaaactct tgaacgagac aggagaactc tttgagccat ctatgaacga
     5821 atctcgcaag gaacaactct acaagggctg gaagaaggct gtgaaagcaa ctcaagtctt
     5881 tgcggaaatc gatgactaat actcaatgaa aatcaaagag caaactagga agctagccgc
     5941 aagctgtact tgagtacggt aaggcgacgc tgacgtggtt tgaagagatt ttcgaagagt
     6001 ataatactgg cagaataaag cgatttagtt agaaagtgtg taaatatgga attttcaaag
     6061 aaaacacgtg aattgtcaat taaaaaaatg caggaacgca ccctggacct cttgattatc
     6121 ggtggaggaa tcacaggagc tggtgtagcc ttgcaggcgg cagctagcgg tcttgagact
     6181 ggtttgattg aaatgcaaga ctttgcagaa ggaacatcta gtcgttcaac aaaattggtt
     6241 cacggaggac ttcgttacct caaacaattt gacgtagaag tggtctcaga tacggtttct
     6301 gaacgtgcag tggttcaaca aatcgctcca cacattccaa aatcagatcc aatgctctta
     6361 ccagtttacg atgaagatgg agcaaccttt agcctcttcc gtcttaaagt agccatggac
     6421 ttgtacgacc ttttagcagg tgtgagcaac acaccaactg cgaacaaggt tttgagcaag
     6481 gatcaagtct tggaacgcca gccaaacttg aagaaggaag gcttggtagg aggtggagtg
     6541 tatcttgact tccgtaacaa cgatgcgcgt ctcgtgattg aaaacatcaa acgtgccaac
     6601 caagacggtg ccctcattgc caaccacgtg aaggcagaag gcttcctctt tgacgaaagt
     6661 ggcaagatta caggtgttgt agctcgtgat ctcttgacag accaagtatt tgaaatcaag
     6721 gcccgtctgg ttattaatac aacaggtcct tggagtgata aagtacgtaa tttgtctaat
     6781 aagggaacgc aattctcaca aatgcgccca actaagggag ttcacttggt agtggattca
     6841 agcaaaatca aggtttcaca gccagtttac tttgacacag gtttgggtga cggtcgtatg
     6901 gtctttgttc tcccacgtga aaacaagact tactttggta caactgatac agactacaca
     6961 ggtgatttgg agcatccaaa agtaactcaa gaagatgtag attatctact tggcattgtc
     7021 aacaaccgct ttccagaatc caacatcacc attgatgata tcgaaagcag ctgggcaggt
     7081 cttcgtccat tgattgcagg gaacagtgcc tctgactata atggtggaaa taacggtacc
     7141 atcagtgatg aaagctttga caacttgatt gcgactgttg aatcttatct ctccaaagaa
     7201 aaaacacgtg aagatgttga gtctgctgtc agcaagcttg aaagtagcac atctgagaaa
     7261 tatttggatc catctgcagt ttctcgtggg tctagcttgg accgtgatga caatggtctt
     7321 ttgactcttg ctggcggtaa aatcacagac taccgtaaga tggctgaagg agctatggag
     7381 cgcgtggttg acatcctcaa agcagaattt gaccgtagct ttaaattgat caattctaaa
     7441 acttaccctg tttcaggtgg agaattgaac ccagcaaatg tggattcaga aatcgaagcc
     7501 tttgcgcaac ttggagtatc acgtggtttg gatagcaagg aagctcacta tctggcaaat
     7561 ctttacggtt caaatgcacc gaaagtcttt gcacttgctc acagcttgga acaagcgcca
     7621 ggactcagct tggcagatac cttgtccctt cactatgcaa tgcgcaacga gttggctctt
     7681 agcccagttg acttccttct tcgtcgtacc aaccatatgc tctttatgcg tgatagcttg
     7741 gatagcatcg ttgagccagt tttggatgaa atgggacgat tctatgactg gacagaagaa
     7801 gaaaaagcaa cttaccgtgc tgatgtcgaa gcagctctcg ctaacaacga tttagcagaa
     7861 ttaaaaaatt aagaaaaaat aaaagaggtg gagggcagca ttccttgtcg cccgtccctt
     7921 ctttttaatg gagacagaaa gatgatgaat gaattatttg gagaatttct agggacttta
     7981 atcctgattc ttctaggaaa tggtgttgtt gcaggtgtgg ttcttcctaa aaccaagagc
     8041 aatagctcag gttggattgt gattactatg ggttggggga ttgcagttgc ggttgcagtc
     8101 tttgtatctg gcaagctcag tccagctcat ttaaatccag ctgtgacagt tggtgtagct
     8161 ttaaaaggtg acttgccttg ggcttccgtt ttgccttata tcttagccca gttcgcaggg
     8221 gccatgctgg gtcagatttt ggtttggttg caattcaaac ctcactatga ggcagaagaa
     8281 aatgcaggca atatcctggc aaccttcagt actggaccag ccatcaagga tactgtatca
     8341 aacttgatta gcgaaatcct tggaaccttt gttttggtgt tgacaatctt tgctttgggt
     8401 ctttacgatt ttcaggcagg tatcggaacc tttgcagtgg gaactttgat tgtcggtatc
     8461 ggtctatcac taggtgggac aacaggttat gccttgaacc cagctcgtga ccttggacct
     8521 cgtatcatgc acagcatctt gccaattcca aacaagggag acggagactg gtcttacgct
     8581 tggattcctg ttgtaggccc tgttatcgga gcagccttgg cagtgcttgt attctcactt
     8641 ttctagttta tactcttcga aaatcaaatt caaaccacgt cagcgtcgcc ttaccgtact
     8701 caagtacagc ttgcggctag cttcctagtt tgctctttga ttttcattga gtattagaaa
     8761 acaattattt tgatagagct tgggcaagag cccaatttca gcaaaaaatg aagtaaatct
     8821 tctcataata aaacgcatca tatcaagcac gaaaattcca cgaggtcaac tacagtcaga
     8881 aagctgaaca acaagccaaa acgcccaaaa aaggcggcaa aaagcaagca cctgcaagca
     8941 acgtgccgaa atggtcaaat cctgattatg tcaacgaatt agacccaaaa atcgttgata
     9001 tgctagtaga atttcacaag tcacaaggca ctttggaaac tcccgaggcg caagcagaaa
     9061 tcgcccaaaa acgtgaagaa atcgagcaaa ggagagctga gcttgaggat aaaaaacaag
     9121 agcttttgaa ccgcttgaac aaatagagtt tcgcaagtat tatgcttaca aattacttga
     9181 gcaattaact aaaatataaa ccctgccttt atatctaggc agggtttata ttttagaaat
     9241 tcacgtaggt tgttacggtt tttacatacc cagtatagtt tgagtttcta tagtattcag
     9301 tgataaactt ccattttctt tgagcaacat ggatataagt acttgttatg tagtatggat
     9361 atgggctttg tgaatccaag taagactgat aagcttgtat accaaaatat gctccaccaa
     9421 ttattgcacc ccatggaccc cccaataaag cacctatcct accaatcata taactgattc
     9481 cagcaccagt catgaagtta gcgaatgtgt tagcttgttt attcccatgt attgtgttga
     9541 cgtaattcca aacattagga tcgtatgatc taaaagatat atttaggtcg atttcattct
     9601 tttgataagc catataaaat gccccattga tatagacgcc gtcagcacgt cgttcaatag
     9661 tgtctacact tccatctgga ttgacaacct caagaacttc atcgcttaaa atatttactt
     9721 gcgtatctcc gaaccgcact gatgagccat tctcaaactg agcctcacca gatacaactt
     9781 tagagtttgc cgataagcta tcatcagcaa aaacaacaag cgacggggaa atgctagaca
     9841 tacagaaaac agacataact agcaaacaca tgcatttaaa catcttagac ataacggaaa
     9901 ctcttttgta tttttgattt ttttcaactt ttattataca ataaaaccaa ataaaaagaa
     9961 agcggtaaca atatgcttaa tgcgaaaatt ttttatatat ttttatgttt gatcgttatc
    10021 gaaactacag gcttgttgtt gttgaaaaga ggtctcgaaa tgggttattt agacacagaa
    10081 gctattatcc tcgcagtttt ttcatttgct ttttacaacc tatgttcatt cgcttgggtc
    10141 tgctctacaa taaaaaataa atagacgtat tttcaaaaaa aaaaaaatgc atatttatat
    10201 tagcaaaatg acgatttaaa tcgtcgtttt tttgtagtac gacgggcatg tcgtatatct
    10261 gaggtgtaag tcctcggtgg gcacctgcta ccggtgaacc caatagcgat tcccaagcct
    10321 gactatcgtg aggtagcagg gagaggaagg gatagcgaaa tcgtggctct acgaacagga
    10381 acgtgatagt aaggcgtata tagcggataa ggaggcttca aactctaaag tccaaaaagg
    10441 tagtcgtaac ctatatgtgt aaatcacgag agtaattgaa ttcggactaa ggtttgtgtg
    10501 aaaaagataa atctttctag agtctaaaga ctctgcgtca gatttcctat tttcactgta
    10561 accttttaac gtcctcatat cttgtataaa cgaggaaaga tgtacgactt atcccgtgag
    10621 gtttcatgag cgctgaaagc gtagtaacaa cgaatcatga gaagtcagcc gagcccatag
    10681 tagtgaggaa acttctgtag tggaagtgga gcgaaggggt gaatactcaa acagtctggg
    10741 gagagactgt ttgaggtctg tcgctagaaa gagaaaacga ca
//
LOCUS       DBERDK010000063        10684 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.063, whole genome shotgun sequence.
ACCESSION   DBERDK010000063 DBERDK010000000
VERSION     DBERDK010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 10684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10684
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.063"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            52..372
                     /locus_tag="ACL73L_001633"
                     /pseudo
     CDS             52..372
                     /locus_tag="ACL73L_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884744.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
     gene            493..1437
                     /gene="manA"
                     /locus_tag="ACL73L_001634"
     CDS             493..1437
                     /gene="manA"
                     /locus_tag="ACL73L_001634"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HGS0772580.1"
                     /translation="MSEPLFLQSVMQEKIWGGAKLRDEFGYDIPSEKIGEYWAISAHP
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEGELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDALLTKVPVKAG
                     DFFYVPSGTMHAIGAGILILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTIKADDLRSTLLVSNDFFAVYKWEITGKVNFEKTADYSLLSVLAGQGQL
                     TVDGKNYPIQKGRHFILPSDVEAWTLEGQDLELIVSHP"
     gene            complement(1428..2823)
                     /locus_tag="ACL73L_001635"
                     /pseudo
     CDS             complement(1428..2823)
                     /locus_tag="ACL73L_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844893.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
     gene            3029..3774
                     /locus_tag="ACL73L_001636"
                     /pseudo
     CDS             3029..3774
                     /locus_tag="ACL73L_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274781.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            complement(3803..4258)
                     /locus_tag="ACL73L_001637"
     CDS             complement(3803..4258)
                     /locus_tag="ACL73L_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HGS0772581.1"
                     /translation="MNRREAVEFVNMCMIKNGDKVLVQDRVSPDWSGITFPGGHVERG
                     ESFVDAVIREVKEETGLIISKPQLCGIKNWYDDKDYRYVVLFYKTEHFTGELQSSDEG
                     KVWWEDFENLSHLKLATDDMSDMLRVFLEEDLSEFFYYKNGDDWLYDLK"
     gene            complement(4278..5453)
                     /locus_tag="ACL73L_001638"
     CDS             complement(4278..5453)
                     /locus_tag="ACL73L_001638"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HGS0772582.1"
                     /translation="MKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAG
                     TGGGRCNVTNNGSLDNLLAGIPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGR
                     VFPASDKSRTIIEALEKKITELDGQVATQIEIVSVKKVDDQFVLKSADQTFTCEKLIV
                     TTGGKSYPSTGSTGFGHEIARHFKHTITDLEAAESPLLTDFPHKALQGISLDDVTLSY
                     GKHVITHDLLFTHFGLSGPAALRMSSFVKGGEVLSLDVLPQLSEKDLVTFLEENREKS
                     LKNALKTLLPERLAEFFVQGYPEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFV
                     TKGGVSLKEINPKTLESKLVPGLHFAGEVMDINAHTGGFNITSALCTGWVAGSLHYD"
     gene            complement(5512..6357)
                     /locus_tag="ACL73L_001639"
     CDS             complement(5512..6357)
                     /locus_tag="ACL73L_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HGS0772583.1"
                     /translation="MTWKIIADSGCDYRQLPTPAINTTFVSVPLTIQVADQVFVDDAS
                     LDIDQMMETMYATAEASKSACPSPDDYLRAFEGAKNIFLVTITGTLSGSHNSAQLAKN
                     IYLEDHPDTKIHVIDSLSAGGEVDLLVEKLNDLIDQGLSFEEVVEAITAYQEKTKLLF
                     VLAKVDNLVKNGRLSKLIGTVVGLLNIRMVGKASETGTLELLQKARGSKKSVQAAYDE
                     LVKAGYAGGRIVMAQRNNEKCCQQLSERIRETFPQADIKILPTSGLCSFYAEEGGLLM
                     GYEID"
     gene            6482..7039
                     /locus_tag="ACL73L_001640"
     CDS             6482..7039
                     /locus_tag="ACL73L_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGS0772584.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFREARKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
     gene            7058..7525
                     /locus_tag="ACL73L_001641"
     CDS             7058..7525
                     /locus_tag="ACL73L_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HGS0772585.1"
                     /translation="MTEKRLAWDEYFAAQALLIANRSTCKRAKVGAILVKDNKVISTG
                     YNGSVSGTEHCIDHECLVIEGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNC
                     TKQLLQVGCKRVVYINQYRMDDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
     gene            7614..8243
                     /gene="upp"
                     /locus_tag="ACL73L_001642"
     CDS             7614..8243
                     /gene="upp"
                     /locus_tag="ACL73L_001642"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HGS0772586.1"
                     /translation="MGKIEVINHPLIQHKLSILRRTDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRDEETLQPVEYLVKLPEDIDQRQIFVVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKALQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            8417..9007
                     /gene="clpP"
                     /locus_tag="ACL73L_001643"
     CDS             8417..9007
                     /gene="clpP"
                     /locus_tag="ACL73L_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HGS0772587.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SMEKVHADAERDNWMSAQETLEYGFIDEIMANNSLN"
     gene            9086..9334
                     /locus_tag="ACL73L_001644"
     CDS             9086..9334
                     /locus_tag="ACL73L_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HGS0772588.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQELETPFVGNLYR"
     gene            9435..10595
                     /locus_tag="ACL73L_001645"
     CDS             9435..10595
                     /locus_tag="ACL73L_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGS0772589.1"
                     /translation="MKKKFALSFVALASVALLAACGEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVVDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDF
                     QAALTKMKGKDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKDNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP"
BASE COUNT         3377 a         2124 c         2287 g         2896 t
ORIGIN      
        1 gttgatttca tatcactatt atataatgct ttttgatttt agtttagtat tatcgtagaa
       61 aatggactcg gtgctatgga tacaccggat gagaatggtt atgtagcaga taactatcgg
      121 attacttact tagaggccca catcaaggcc atgcgagatg ccatttacca agacggggtt
      181 gacttgcttg gttatacgac ttggggctgt atcgatctgg tttcagctgg cacaggggaa
      241 atgaataagc gctatggctt tatctatgta gatcgagata atgtaggaaa cggagctctc
      301 aaacgtagca agaagaagtc cttctattgg tacaaggatg tcattgatag caatggtgca
      361 agcatcgggt agagcacatt tgtttactat ttttataaaa tcttctccct gctttataag
      421 atgtaaaaaa gagttcaatt cgaactcttt ttttgctata atgaggggag aaaaatcaga
      481 caggagatcg acatgtcaga accattattt ttacaatcag ttatgcaaga aaaaatctgg
      541 ggtggagcca agctacgtga tgagtttggc tacgacatcc caagtgaaaa aatcggagaa
      601 tattgggcca tctcagccca tccaaatgga gtctctaaag ttgccaatgg tcgttacgag
      661 ggaacagatc ttgctacttt gtatgcggaa caccgtgaat tatttggcaa tcgtccagaa
      721 cctgtatttc cacttttgac caagatcctc gatgccaacg actggctcag tgtccaagtt
      781 cacccagacg atgcttatgg actcgagcat gaaggcgaac tcggaaaaac agaatgctgg
      841 tacattatcg cagcggatga aggttcagag attatctacg gtcacaatgc caagtcaaaa
      901 gaagaactcc gccagcaaat cgaggataag aactgggatg cgctgttgac aaaagtgcca
      961 gttaaagcag gagatttctt ctatgtacca agcggtacca tgcacgctat cggggcgggt
     1021 atcttgattc ttgaaaccca gcagtctagc gataccactt accgtgtcta tgactttgac
     1081 cgtaaggatg acaagggcaa tttacgtgaa cttcaccttg aaaaatctat cgatgtcttg
     1141 aacattggtg aacccgcaaa tagccgtcca gtgacaatca aagcagatga tctacgttcc
     1201 actctccttg tatccaatga tttcttcgca gtttacaaat gggaaattac tggaaaagtt
     1261 aactttgaaa agacagctga ctacagcttg ttgagtgtct tggctggtca aggtcaacta
     1321 actgttgacg ggaaaaacta tccaatccaa aaagggagac actttatcct accaagtgat
     1381 gttgaagctt ggactctgga agggcaagat ttagaattaa ttgttagtca tccataaaaa
     1441 agaaagggcc tgagttcatt actcaagtcc ttttgattac ataaattggg cgatgaagac
     1501 cacgatgatg attggaatga cggaacgaag aaacgaagaa ggaagagcca gacttggaac
     1561 agtccttgtt tccatgctct ttcatcgaga tggagttcct ccattgcaag agccttttta
     1621 aagatatagc ctgtaaaaag tgaaaggtag agagctccaa atggcatgag gagattggaa
     1681 accaagaagt ccatagcgtc aaagaaggtc ttaccaaaaa tgtgaacatc cgccatgaca
     1741 ccgtaagata gggctgaagg aatgccaaag acaaaggtca aaattcctaa aataacactc
     1801 catttggcac gtttgctgtt atcctggttg gtgatattgc ccacattgat ctccagcatc
     1861 acgacagaag aagtgacagt cgcaaagagg aagaggacgt agaaaatggt tccaaaaggc
     1921 atcttgtcaa agagttgagg caagacgata aagagcaagc tcggtccccc ttcagactgg
     1981 atattgaagg gggatcgagc ttggaaaatg gctagacctg ccatgatgga tatcgagata
     2041 ttcatggcta cgatggagat tcctgactgg actagattgg ttttcttgtc caagtaagga
     2101 gcataggtca acatgactgt aacccctagt gagagggcaa agaaagattg tcccagagca
     2161 tagaggagac cagcgctagt cagttttgaa aagtctggtt tgaggaagta aagaaccctt
     2221 ccatggcatt tggcaaactg agagagcgac cgatgataac aacaaagatg ataaagagca
     2281 ggggcatcat gactttcgaa gctctttcaa tccctttttg aacaccacgt gatacaataa
     2341 agatatttaa taagataaag gctgcttgag ctactagggc aatggctgga tttgaaatga
     2401 ttgaagtaaa taactgagca taatcacccg ttccaccaag ttggaacaat ttcccaaact
     2461 caatacctag atagactaga atccatcctc cgataacact gtaaaaagat aagaggataa
     2521 agagggcaaa ggcgccaatc caaccgataa agttgtactt gttattcttg cccagttttc
     2581 caaaggtttt gatagcggaa acgccagcca ctacggccaa gggcaaactc agccagcagg
     2641 agagggaaac cgattaaaat agtggaaatg agaaagatta gtaaaaagcc tccaccgcca
     2701 ttagcagcag tcatgtaggg aaacttccaa acggaaccaa gcccgatgac cgagcccgat
     2761 gaccgagcca gcagatgcta gaataaagcc aagtttcgac ccccattgtg atttttctga
     2821 catagttaaa ctctgatctc ctttttcaaa ataagaaaaa gctacttgag ttgtcaaata
     2881 gccttctgtt tgattgatag acttgactat cctattatgt tttctaaggt ctgtcaagaa
     2941 aaccattttc aagttttcac acctttctca aaaacgcttg actctgacct aaggggaggg
     3001 gttatactat cattgtaagg aggaaatcat gtaccatata aaagaagctg cgcagctttc
     3061 aggtgtctct gtcaagaccc tgccctgcat cactatgaca agataggact cttggtcccc
     3121 ttaaagtcgg aaaacggcta tcgaacctat agtcaagagg atttggaacg ccttcaggtc
     3181 attctttact acaaatatct aggcttttct ttagagaaaa tagcagaact gttaaaggaa
     3241 gaaaggacag atttattgcc ccatttgact aggcagttgg actatctaac tcgcgaaagg
     3301 caacatctgg ataccttgat ttccaccttg caaaaaacta ttcaagaaca aaaaggagaa
     3361 agaaaaatga ccattgagga aaaattcacg ggatttagct atcaagacaa tcaaaaatac
     3421 caccaagaag cggtagagaa atatggtcaa gaagtcatgg gacaagcgct cgaacgccaa
     3481 aaaggtcacg aagacgaggc tacggccgcc tttaaccaag tctttcaaac tttggcacaa
     3541 aatcttcaag ttggtttacc tgcaacagca accgaaaacc aggagcaagc agccaagctc
     3601 ttgcaagcca ttcgcactta tggatttgac tgctctattg aggtattcgg tcatatcggt
     3661 aaaggttacg tctacaaccc agagtttaag gaaaacattg acaagtttgg ttctgaaaca
     3721 gcccagtaca cgtcagatgc cattgcggct tacgttcaga caaatgcaga ataaataggc
     3781 taggaatttc ctagcctatt ttttacttca aatcataaag ccagtcgtca ccgtttttgt
     3841 agtaaaagaa ttcactgaga tcttcttcta gaaacacacg aagcatatca gacatatcat
     3901 cggttgcaag ttttagatga gaaagatttt caaagtcctc ccaccaaact ttcccttcgt
     3961 ctgaagactg gagttcacca gtaaagtgtt ctgtcttgta aaaaaggacg acataacgat
     4021 aatccttgtc gtcataccag tttttgatac cacagagttg gggtttggaa atgatcagac
     4081 cagtttcttc tttcacttca cgaatgacag catcgacaaa ggattcgcca cgttcaacat
     4141 gaccaccagg aaaagtaatg ccagaccagt cgggactaac tcggtcttgg accaggacct
     4201 tatctccgtt tttaatcata cacatgttaa caaattcgac tgcctctctt ctgttcattc
     4261 ttcacaacct ttaatcttta atcataatgc agacttcccg ccacccagcc ggtacagagg
     4321 gcagaagtga tgttaaagcc acccgtgtgg gcattgatat ccataacttc gcctgcaaag
     4381 tggaggccag gtaccagctt actttcaagg gttttaggat tgatttcctt gagactgact
     4441 ccacccttgg taacaaagga ctttgcaagg gacatttttc cagttacagg aattttaagt
     4501 tctttaatgg actggacaag ttgttctcgt tccttttcag tcagttgttt gactttttca
     4561 ggatatcctt gtacaaaaaa ttcggccaag cgttctggta acaaggtttt taaagcgttt
     4621 ttcaaggatt tttcccgatt ttcttctaga aatgtaacca agtccttctc agaaagttga
     4681 ggcaaaacat cgagtgagag aacctcccca cctttgacaa agctagacat gcgtagggca
     4741 gcaggacctg acaaaccaaa gtgggtaaag agtaaatcat gagtgatgac atgcttacca
     4801 taacttaggg tcacatcgtc cagagaaatc ccttgtaagg ctttatgtgg aaaatctgtt
     4861 aataaaggac tttcagcagc ctcaagatcg gtgatggtat gcttaaaatg gcgagcaatc
     4921 tcgtgaccaa aaccagtcga accagtcgaa ggataagact taccacctgt tgtgacaatg
     4981 agtttctcac aagtgaaggt ttgatccgct gacttaagga caaactggtc atctactttt
     5041 ttaacagaaa cgatttctat ttgagtagca acttgaccat ctagttcggt gattttcttt
     5101 tccaaagctt cgataatagt ccgagacttg tcactggctg gaaagacgcg tccgtggtct
     5161 tcgaccttaa gtttaacacc attttctgta aaaaagttga tgatgtcatg attatcgaac
     5221 tgggagaaaa cactgtaaag aaagcgtccg tttccaggaa ttccagctag caggttgtct
     5281 aagctaccat tgttggtcac attgcaacgt cccccaccag tcccagctaa tttttttcca
     5341 agtttccgat ttttttcgat gaggagggtt ttctgtccat aaaagctact ggaaatcgta
     5401 gccatcatac cagcaggtcc cccaccgatg acaatagtat caaaatgttt catagctcta
     5461 ttgtaccaca aaaaaacaag agatgatggt cacctcttgt caagaatgca attaatcaat
     5521 ttcatagccc atcagcaaac cgccctcttc tgcatagaaa ctgcagagac cagaggttgg
     5581 tagaatttta atatccgctt gtgggaaggt ttcacggatt cgctctgaga gctgttgaca
     5641 acatttttcg ttattgcgtt gggccatgac aatacggcca ccagcatatc cagcttttac
     5701 taactcatca taggcagctt gaactgattt ctttgatccc cttgcttttt gtagcaattc
     5761 gagagtccca gtttcactag cttttccgac catacgaatg ttgagaaggc caacgaccgt
     5821 accgataagc ttgctcaaac ggccgttctt caccaagtta tcgactttgg ctaggacaaa
     5881 gagcaactta gttttttctt gataggcggt gatagcttca accacttctt caaaagacaa
     5941 gccctggtca atcaagtcat tcaatttttc tacgagtagg tcaacttcac caccagcaga
     6001 taaactatca atcacatgaa tcttagtgtc aggatggtct tccagataaa tattctttgc
     6061 tagttgagca ctattgtgac tgccagaaag ggtacctgtg atggttacta ggaaaatgtt
     6121 tttggcacct tcaaatgctc gcaaatagtc atctgggctt ggacaagctg attttgaagc
     6181 ttctgcagtt gcatacatgg tttccatcat ttggtcaata tcgagactgg cgtcatcaac
     6241 aaagacctga tcagctactt gaatggttaa ggggacactt acaaaggttg tgttaatagc
     6301 tggtgttggc agttgacgat aatcacaacc agagtcagca ataatcttcc aagtcataga
     6361 aattctccat ctttgtcagt tatacattga caaaggttct gtcttttttt acaattatat
     6421 catgaaaact cttgaaacaa aagcatcatc cgtctgttgt gacaagtaga aagaaaatgt
     6481 tatgtctgaa cgtagaatct ctgaaaagtc tcttgaaaat ctcagaaaat caaaccaaga
     6541 atccaattta ttaaccagag aagccattga aacagccctc ttgcaactct tggaaaaaaa
     6601 ggagctgacc aagattagta tttctgaatt ggtcaaacgt gcaggtgttt cgcgtgcggc
     6661 cttttatcgc aattatgact ccaaagagga aattttagag agcgtcttta aacgaactgt
     6721 ccacaatatt atggaacagc tgcatcatta cgatttaaag acagaccttt atttggtttg
     6781 ggttcacctt ttccgggagg ccagaaagga agctagagta attcaattgg ccttggatta
     6841 ccatctggaa aaaatctttg tccaagccat gcaggaattt ctagaaaaat accatgggaa
     6901 atcaaaaggt gtcagctctt atcttcattc cttctggagc tcagccatcg tctctgtcct
     6961 tctaaaatgg atcaaggatg gcatgaaggt accagctgaa aagattgcag atttaggttt
     7021 accatttttt aaaaaataga gaaaaaggag aaaagagatg actgaaaaaa gactggcctg
     7081 ggatgagtat tttgcagccc aagctctact aattgcgaat cgttccactt gtaaacgtgc
     7141 taaagtgggc gcgattctgg taaaagataa taaggttatt tccactggtt acaatggttc
     7201 ggtgtcaggg actgagcatt gtattgatca cgaatgtttg gtcattgaag gccactgtgt
     7261 tcgcaccctt cacgctgagg tcaatgctat ccttcaaggt gctgagcgtg gtgttcccaa
     7321 aggctttaca gcctatgtaa cccattttcc ttgtctcaac tgtacaaaac aattgcttca
     7381 ggtcggctgc aagcgcgtgg tttatatcaa ccagtaccga atggacgact acgcccaata
     7441 cctttatcaa gaaaaaggga cagaattgac ccatttacca cttgagacag tacagacagc
     7501 tcttaaagag gctgatctaa tgtaaaaatt atcaaaaata aatggtttag aaagattttt
     7561 aaaccgtttt ttggtataat aagaagaata aattgaaaga aggaattcca aaaatgggaa
     7621 aaattgaagt tattaatcac ccactgattc aacacaaatt gtcaatcttg cgtcgtacag
     7681 atacttctac aaaagctttt cgtgagctag tagatgagat tgcaatgttg atggggtatg
     7741 aagtacttcg tgatcttcca ctagaagatg tggaaatcga aacaccaatt acaaaaacag
     7801 ttcaaaaaca attggcaggt aagaaattgg ccatcgtccc aatcttgcgt gcaggtatcg
     7861 ggatggttga tggtctcttg agcttggttc cagctgctaa agttggccac atcggtatgt
     7921 accgtgatga agaaacactt caaccagttg aatacttggt gaaattgcct gaggacattg
     7981 accaacgtca aatttttgta gtagacccaa tgttggcaac aggtggctca gcaatcttgg
     8041 ctgttgattc tcttaaaaaa cgtggcgcat caaatatcaa atttgtctgc cttgtatctg
     8101 ctccagaggg tgtaaaagcc cttcaagaag ctcatccaga tgtagaaatc tttacagcag
     8161 ccttggatga acgtttgaac gaacacggtt atatagttcc aggtcttgga gatgctggag
     8221 accgcttgtt cggtacaaaa taagatcgaa aagaagaatg acttggaaaa gtatttctag
     8281 tcacgaaagg aggttgggtt tttgtttctg tctaatgaaa gcagagcaaa aatttgacct
     8341 tttttgacca agatattata atagtcttat cttcagtcat ttgaccaaca aaattaaaac
     8401 tcaaaaggag aaatgaatga ttcctgtagt tattgaacaa acaagccgtg gagaacgttc
     8461 ttacgatatt tactcacgtc ttctcaaaga ccgcatcatt atgctgacag gtccggttga
     8521 agacaatatg gctaactctg ttattgccca attgcttttc ttggatgccc aagatagtac
     8581 gaaagatatt tacctttatg tcaatacacc aggtggttct gtttcagctg gtttggcaat
     8641 cgtagatacc atgaacttta tcaaggcaga tgtccaaacc attgttatgg gaatggctgc
     8701 atctatgggg actgtcatcg catcaagtgg agcaaaaggc aaacgtttca tgcttccaaa
     8761 tgctgaatac atgattcacc aaccaatggg cggtacaggt ggtggtaccc aacaaactga
     8821 tatggctatc gctgcagaac acttgctcaa aactcgtaat accttggaaa aaatcttggc
     8881 tgaaaattca ggtcagtcaa tggaaaaagt ccatgcagat gcagaacgtg ataactggat
     8941 gagcgcccag gaaacacttg aatatggctt tattgatgaa attatggcca acaattcatt
     9001 gaactaatga tgatagaagg caaactcgac tgggtttgct ttttttggta taatagggag
     9061 agatttctta gaaagaggat ttatcatgtt tgaaaaagtc aatcgctctg gcttgattat
     9121 ctatctttac tataatcgtg atgccaaaaa actgcaggat tatggagata ttacctatca
     9181 ttccaagaaa catcgttact tacaactcta tgttccaact caagaagtgg agcaattggt
     9241 cggacgcttg agcaaggaaa aatttattaa aaaagttagg gtttgtcata tccaagagtt
     9301 ggaaacaccc tttgtgggca atctttatcg ataggaaaac gttatcatcg aaaaagttca
     9361 aggaaaacat tgacaatttt ctgataattc ggtatattct taacagacta tttaagaaat
     9421 aaggagacaa aaagatgaag aaaaaatttg ccctatcgtt tgtggcgctt gcaagtgtag
     9481 cacttcttgc agcctgtgga gaagtgaagt ctggagcagt caacactgct ggtaactcag
     9541 tagaggaaaa gacaattaaa atcgggttta actttgaaga atcaggttct ttagctgcat
     9601 acggaacagc tgaacaaaaa ggtgcccaat tggctgttga tgaaatcaat gccgcaggtg
     9661 gtatcgatgg aaaacaaatc gaagtagtcg ataaagataa taagtctgaa acagctgagg
     9721 ctgcttcagt tacaactaac cttgtaaccc aatctaaagt atcagcagtc gtaggacctg
     9781 cgacatctgg tgcgactgca gctgcggtag cgaacgctac aaaagcaggt gttccattga
     9841 tctcaccaag tgcgactcaa gatggattga ctaaaggtca agattacctc tttattggaa
     9901 ctttccaaga tagcttccaa ggaaaaatta tctcaaacta tgtttctgaa aaattaaatg
     9961 ctaagaaagt tgttctttac actgacaatg ccagtgacta tgctaaaggg attgccaaat
    10021 ctttccgcga gtcatacaag ggtgaaatcg ttgcagatga aactttcgta gcaggtgaca
    10081 cagactttca agcagccctt acaaaaatga aagggaaaga ctttgatgct atcgttgttc
    10141 ctggttacta taatgaggct ggtaagattg taaaccaagc gcgtggcatg ggaattgaca
    10201 aaccaatcgt tggtggtgat ggattcaacg gtgaggagtt tgtacaacaa gcaactgctg
    10261 aaaaagcatc aaacatctac tttatctcag gcttctcaac tactgtagaa gtttcagcta
    10321 aagctaaagc cttccttgac gcttaccgtg ctaagtacaa tgaagagcct tcaacatttg
    10381 cagccttggc ttatgattca gttcaccttg tagcaaacgc agcaaaaggt gctaaaaatt
    10441 caggtgaaat caaggataac cttgctaaaa caaaagattt tgaaggtgta actggtcaaa
    10501 caagcttcga tgcagaccac aacacagtca aaactgctta catgatgacc atgaacaatg
    10561 gtaaagttga agcagcagaa gttgtaaaac cataatagaa aaatgttgaa atagggaatg
    10621 agcctttgac tcactccctg tttcgatatt taatactctt cgaaaatctc ttcaaactgc
    10681 gtca
//
LOCUS       DBERDK010000064        10536 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.064, whole genome shotgun sequence.
ACCESSION   DBERDK010000064 DBERDK010000000
VERSION     DBERDK010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 10536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10536
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.064"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(23..1447)
                     /locus_tag="ACL73L_001646"
     CDS             complement(23..1447)
                     /locus_tag="ACL73L_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HGS0772590.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAIERAAYLHKTAAILERDKEKIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAMEGGGFEAASKNKLAVVRREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMFKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIE
                     HKEVNFINFTGSTPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHTAKQIVAGAF
                     SYSGQRCTAIKRVIVLESVADKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIW
                     GLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVAWEEPFGPVLPIIRVASVEE
                     AIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHINNKTQRGPDNFPFLGVKGSG
                     AGVQGIKYSIEAMTNVKSIVFDVK"
     gene            complement(1992..4271)
                     /gene="pulA"
                     /locus_tag="ACL73L_001647"
     CDS             complement(1992..4271)
                     /gene="pulA"
                     /locus_tag="ACL73L_001647"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051060 - pullulanase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HGS0772591.1"
                     /translation="MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKTYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAAFQTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLAPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGSYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSY
                     SSYDEIYHHVFVHSAIEHSGYLIYEVHGKEHLLVVVNAKSEPYQFENAGNLAMLVTNS
                     RSKEDNVLNDISLAVLSVL"
     gene            complement(4383..6341)
                     /gene="ligA"
                     /locus_tag="ACL73L_001648"
     CDS             complement(4383..6341)
                     /gene="ligA"
                     /locus_tag="ACL73L_001648"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HGS0772592.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVADSEYDRLYRELVELET
                     AHPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQVRQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KVSQLLLQYFHSIENLSQADSEEVASIESLGGVIAKSLQTYFATEGSEILLRELKETG
                     VNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSVSKKTDLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
     gene            complement(6434..7612)
                     /locus_tag="ACL73L_001649"
     CDS             complement(6434..7612)
                     /locus_tag="ACL73L_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGS0772593.1"
                     /translation="MNRYAVQLISRGAINKMGNMLYDYGNSVWLASMGTIGQTVLGMY
                     QISELVTSILVNPFGGVISDRFSRRKILMTADLVCGILCLAISFIRNDSWMIGALIVA
                     NIVQAIAFAFSRTANKAIITEVVEKDEIVIYNSRLELVLQVVGVSSPVLSFLVLQFAS
                     LNMTLLLDSLTFFIAFVLVAFLPKEEAKVQEKKAFTGRDIFVDIKDGLHYIWHQQEIF
                     FLLLVASSVNFFFAAFEFLLPFSNQLYGSEGAYASILTMGAIGSIIGALLASKIKANI
                     YNLLILLALTGVGVFMMGLPLPTFLSFSGNLVCELFMTIFNIHFFTQVQTKVESEFLG
                     RVLSTIFTLAILFMPIAKGFMTVLPSVHLSSFLIIGSGVIILSCISFIYVRTHFEKLI
                     "
     gene            complement(7692..8555)
                     /locus_tag="ACL73L_001650"
     CDS             complement(7692..8555)
                     /locus_tag="ACL73L_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HGS0772594.1"
                     /translation="MEHLGKVFREFRTSGNYSLKEAAGESCSTSQLSRFELGESDLAV
                     SRFFEILDNIHVTIENFMDKARNFHNHEHVSMMAQIIPLYYSNDIAGFQKLQREQLEK
                     SKSSTTSLYFELNWILLQGLICQRDATYDMKQDDLDKVADYLFKTEEWTMYELILFGN
                     LYSFYDVDYVTRIGREVMEREEFYQEISRHKRLVLILALNCYQHCLEHSSFYNANYFE
                     AYTEKIIDKGIKLYERNVFHYLKGFALYQKGQCKEGCKQMQEAMHIFDVLGLPEQVAY
                     YQEHYEKFVKS"
     gene            complement(8558..10459)
                     /locus_tag="ACL73L_001651"
     CDS             complement(8558..10459)
                     /locus_tag="ACL73L_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HGS0772595.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVGEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFDDLRRTDKQLR
                     QMELEMGEKSGEDLDKLMSDYDRLSENFRQAGGFTYEADIRAILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETISWLENYLVNYSGALIIVSHD
                     RYFLDKVATITLDLTKHSLDRYVGNYSRFVELKEQKLATEAKNYEKQQKEIAALEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKAANMTFQSEKTSGNVVLTVENTAI
                     GYDGEVLSQPINLDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTPSNTVLDELWNDFKLTPEVEIRNRLGAFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRVAT
                     HVLELSENGSTLYLGDYDYYVEKKATAEMSQTEEASTSNQAKEASPVNDYQAQKESQK
                     EVRKLMRQIESLEAEIEELESQSQAISEQMLETNDADKLMELQAELDKISHRQEEAML
                     EWEELSDQV"
BASE COUNT         3160 a         2334 c         1888 g         3154 t
ORIGIN      
        1 aacaatttcc tggttttaca cgttatttca catcaaaaac aatggatttg acatttgtca
       61 tcgcttcaat gctatattta attccttgca ctccagctcc agaacctttg acaccaagga
      121 atgggaaatt atctggacca cgctgggttt tattattaat gtggactgta cctacttcaa
      181 gtttttcagc aatttcaaag gcttttttga aatcatttgt aaagactgat gattgaaggc
      241 cgaattcaga ttcgttggca aaggcaatag cttcctctac actagccaca cgaatgattg
      301 gtaaaacagg accaaatggc tcttcccatg ccactttcat atcttttgta acttggtcaa
      361 aaagcactgg ccagaggaga ttgccctcac gtttgattgg tgtaagagcc tgagcctctt
      421 tttcttgtgc atcctcaatc aagccccaaa tgaagtcggc tgaagcattg tcaataacag
      481 gtgtaatatc agcattgtca aatggatcac caactgttaa tttagaaact tcttcctgaa
      541 gcaaagtagc taatttatct gctacacttt cgagaacaat gacacgttta atggccgtgc
      601 aacgttgtcc tgagtagcta aaggctcccg caacaatttg cttggcagta tgttccaaat
      661 ctgcatcttc tagtacaaga gctgcatctt tcccaccaag ttccaacatg ataggacgca
      721 taccagctaa acgaccaata cgttctccaa taggagttga acctgtaaag ttgatgaaat
      781 tgacttcttt gtgctcaatg atataatccc caatttctga accacgacct gtaatggtgt
      841 tgaaaacacc tgccggaatc cctgcttctt caaatgcttt agccaacaag agtccagaaa
      901 tggaaccttg tgttggtggc ttaaacatga ccacattccc tgcaatcaag gcaggtgcaa
      961 ttttagaagc agataaatta actggataat taaaaggagc aatcgctagc acgataccaa
     1021 ctggttcacg acggacaaca gccagtttgt ttttacttgc tgcctcaaaa ccaccacctt
     1081 ccattgcttg tccagtgata cggagacctt cctcagcagc ataacgaatc aagtctgctg
     1141 tacgcactac ttctccaatt gctgctttaa tcccttttgc tacttctttg gcaaggatag
     1201 taccaatttt ttccttatcg cgttctaaaa tagctgctgt tttatgcaaa taagccgcac
     1261 gttcaattgc tgataaagct cgccatgctg gcagggctgc acgcgcagct tgcatagcct
     1321 catcagcttc agtctgagtc atggctggaa ctgtacccaa ttcttcttga ttgattggtg
     1381 aataaatcgt aatttcttgt tcagatgatt tccattttcc atttactaaa ttctgatatc
     1441 ttgtcaaatt cttgtcctcc tgaaaatgat taagatatat tttaccaaat tttcagaaaa
     1501 tcacaaccct ttttgcaaaa tttttgagat tattttcaca atcttgattt ttcaaagtat
     1561 actcaataaa aattaaaaaa atccactacg tcaaggcgag gctaatgtgg tttgaagaaa
     1621 ttttcgaaga gcgtgaatga gtatcatcta tagtaaaata aaaaaactga acaatttggt
     1681 tggggacagc caaaccaatt tctcacaatg tttcagaaac aagggtgtgc tattccaatt
     1741 tcagcctact ataactgtca tagattgctg aaacaaagtc tagataaaag tcttcataat
     1801 aaaaagacct cctatcaagt gttcaaaaac tttgatagga ggtcttgttt tgtgaaaata
     1861 tttatcaaat tttctataca agtgagctgt tagccaggtt ctttctattc tttcaatttc
     1921 aatgaatgga ttttttacta atactcataa ctgggaattt gtctgtgtaa aaatagcgag
     1981 atagatggta tttataaaac actcaagaca gctagactaa tatcatttaa aacattatct
     2041 tcttttgagc gactgttggt taccaacata gctaaatttc ctgcattttc aaattgatag
     2101 ggttctgatt tagcattcac aaccaccaag aggtgttctt tgccgtgaac ttcatagata
     2161 aggtagccgc tatgttcaat cgcagaatgc acaaagacat gatggtaaat ttcatcatag
     2221 ctagagtaag aaaaggcacc agtttttgtc ttcaatcgga tgacttgacg gataaactca
     2281 atactgtctt gacgctcatt aatcaagttc cagttcactt ggttcacact gtcaggagca
     2341 ttatagctat tcatcgcacg ctctctatca tcatgggtca actcaccatt ttcaccagtc
     2401 gcaaccagtt tggtacgacc aaattcttga ccgatttcca taaaggccat cccctgcatg
     2461 agcagattca tggctgtggc agtttcgacc ttgcgcatga tttgctctga actttggtct
     2521 ggatgaaggg ttgccaataa atcgtgaaga ttgtaattgt catgggcttc tacatagtta
     2581 agcacctgat ttggatgtgt atagcttcct aattcacgac ttcctaggat tgctttagct
     2641 agaattggct ctgtcgcagc accactgaca aaacctgact tgatagcacc ataaacttct
     2701 ccccctttga cagcatcgcg ctgattgtca ttaaagaaac caatatttgg catctggtag
     2761 gcattgtcct tcttggcctt atcataaggg gcaagacctg ttcccatatc ccatccttct
     2821 ccatagagga taatgttgga gtcgatttca tccaagcttt gacgaatcat ctgcatggtc
     2881 ttgacatcat gaatccccat caagtcaaaa cggaagccgt caatattata ttcctgcacc
     2941 cagtatagaa gagaatcaat catatacttg cgaaacattt cgtgttcact ggctgtttca
     3001 tttccaacac ccgttccatt ctggaaggta ccatctggat tcatacgata atagtaatca
     3061 gggactgttg tttggaatgc tgcatcaaca actgagaagg tatggttata gactacatcc
     3121 ataatgactc caatacccgc atcgtgataa gcttgaacca tcaccttcaa atcacgaatg
     3181 acctgagctg gatcatctgg attagttgaa aaactagttt ctggcgcgtt atagttttgt
     3241 ggatcataac cccagttgta ggttacattt ccatcctcat cgtattcttt atgacggtct
     3301 gcaattggtt gcaattgaac ataattgtag cccagcttct tgatgtaatc aaaagcagtt
     3361 gactggccgt attggttaac tgttccagcc tgagcagcac ccaagaaagt tcctcgaaga
     3421 tgttcatcta cacccgatgt aggtgattta gtcaaatcac gaatatgcat ttcacagata
     3481 actgccttac atggattttc caagcgccaa gtagcctccg aaccgtgctt aacctcgaag
     3541 ttttcaactt gcttttctac atggctcaga atagctgaac gtttgccatc agggctggtc
     3601 gcgattgtat aaggatcacg tgtcagtgtt tggtgatgag ggaattggac ttgatactga
     3661 taagtcttac ctaccaaatc ttcttcaaca tccaaactcc agacaccgat tgtattgtcc
     3721 ttatgattat aagagtagct attgcctctt ttcatctcaa aagtcttcca aacgggtgca
     3781 tcattagcag ctgattcata aacgacaact tgcacttctg tcgctgtagg tgaccagagg
     3841 gaaaaatgag cctgattgtc ctctacacgg caacccaatt ctccttggta accccaatga
     3901 tgatcaaaac tagcactgtt aatggcctta tcaaaggcaa aaggattttg atttttatag
     3961 aaaggactgg caatagcagg attttcagag taataaatcc tatcatcgcc ttccaaaatc
     4021 cagacctctg ttaatagggg atagtgatta aaacggatag aatattcttt actagtttga
     4081 cctgtatgaa ccacaaaatt caagctttct ataacatgtg aacttgggtg ttcaaagcta
     4141 aataaagctc caaaataatc ttctttgtag gttagcaaat caattcgttg atcctgactt
     4201 tttacaaagg agcaagtgtc atattctcca ttcttacgat ggtaatgaat gcgcataggg
     4261 tagttataca ttttttattt ttccttttta ctttgtttct atttcactaa taaatttttg
     4321 tcaatctcgt ctcaattaac agacatagtc atattctcta aactctgttt ttaaacgatc
     4381 cattacaaac tttctagcca tgcctcatct ctgacctgga taccaagttc ttgtgctttt
     4441 tgcagtttac ttccagcgtc tgcacctacc acgacgaggt cggtcttttt agaaacacta
     4501 cctgtcactt tggcacccag actttcgagt ttacttttag cttctgagcg cttgagtcgt
     4561 tccaattttc ctgtcaatac cacggtcaaa cctgacaagg ccgcatccgc tactaccgtc
     4621 tgtcctttat agtccagatt gaccccagtt tctttcaatt ctctgagcag aatttcagag
     4681 ccttctgtcg caaaataagt ctgaagactt ttggcaatca cgccacctag actttcaata
     4741 ctagccactt cctctgaatc tgcctgagac agattttcaa ttgaatggaa atattgaagt
     4801 aaaagctgac taaccttgct tccgacatga cgaattccca aaccaaataa gagcttctcg
     4861 gcagaatttt cctttgatgc ttggatagcc tgatacagtt tagcagcgga cttttcctta
     4921 accccctcta aaaggaggaa atcctcttct tgcaaacgat aaatatccgc cacatccttg
     4981 actaaattag cagcaaaaag cttctcaaca atagatggac caaggcctgt aatattcata
     5041 gcatcacgag aagcaaagtg aatcaagcct tccatgattt gagcagggca acgcggattg
     5101 atacaacgta gggccacttc atcttcaaag tgcaacaagt cagagttaca acttggacag
     5161 tttgtaggga tatctagttt ttcttcagaa acccgtttgg actctaccac acgtaaaacg
     5221 gcagggatga tgtcaccagc cttatataca atgaccgtat cgtcttttcg gatatctttt
     5281 tcagcaatat aatctacatt gtgcagggtc gcacggctaa cagtcgtacc ggcaagttgt
     5341 actggtgtta gattagcagt tggagttaca acaccggtac ggccaactgt ccagtcaact
     5401 gataagagtt gagcttcttt ttcttcggca gggaacttgt aggctactgc ccactttgga
     5461 gccttaactg taaaaccaag ttcttcttga cttgctaggt cgttgacctt gattaccact
     5521 ccatcaatat cgtaaggcag attttcccgt tcctgtccta cttcttggat aaaattccag
     5581 atttcatcta tgttttcagc caagattcgc ttaggattga ccacaaaacc tagttgttct
     5641 aggtacttca aacccttttc ttggctatca cgagttgaag ggctggcttc ttgatagaga
     5701 aacgttgcaa gattacgctt ggcaactact gctgtatcca actgacgcag agttcctgct
     5761 gccgcattac gaggattagc aaattcaggc tctccatttt cttggcgcac ttggttaact
     5821 tggtcaaagg aagcgcgtgg catgtaacat tccccacgaa ctgtgatatc tagttcttct
     5881 ggcaaagtca aagggatgtc cttaacacgc ttgaggtttt ctgtgatatt ttcaccaatt
     5941 gaaccatctc cacgtgttac cccagcaacc aaaatcccct tttcataagt cagcgagata
     6001 gataagccat cgattttcag ctcacaaata taggtcggat gagccacttc cttacgaaca
     6061 cgcgcatcaa aagcatctag ctcctcacgt gaaaaagcat cctgcaaact ataaagagga
     6121 tactgatgac tgtatttttc aaaaccatct aaaaccttgc caccaacacg atgagtcgga
     6181 ctgtctgcta gcacttgctc tggatgagca gtttctaact cgaccaactc acggtaaagg
     6241 cggtcatact cactgtctgc aaccgaggga ttatcgctgg tatagtactc agtcgcatag
     6301 cgattgagca aagcgactaa ctcattcatt cttttattca taagaccatt ttaccataaa
     6361 acaagccctc ctcacaaacg agaagggcgg aaaaaacact tagtttgaaa ttatttttga
     6421 aactcaagca accttatatc aatttttcaa aatgagttcg aacataaata aacgatatac
     6481 aagacaagat gataacacca cttccaatta tcaggaaaga agagagatgt acacttggca
     6541 agactgtcat aaatcctttt gcaataggca taaatagaat agctaaggta aaaattgtac
     6601 tcagtactct tccaagaaat tcgctctcaa ccttggtttg tacttgagta aaaaagtgaa
     6661 tattaaaaat cgtcataaac aattcacaaa ctaaatttcc agaaaaggaa agaaaagttg
     6721 gaagtggtaa tcccatcata aaaactccga cacctgtcaa agccagtaaa atcaaaagat
     6781 tataaatatt agctttaatt ttactagcta gaagagcccc aatgatggaa ccaatagccc
     6841 ccatagttaa aatacttgca taggctcctt ctgacccgta aagctgattc gaaaagggaa
     6901 gtagaaattc aaaagctgca aaaaagaaat taacgctgga agctaccagc aaaaggaaga
     6961 aaatttcttg ctgatgccag atatagtgta acccatcctt gatatctaca aaaatatctc
     7021 tcccagtaaa agcctttttc tcttgaactt ttgcttcctc ttttggaagg aaagccacta
     7081 gaacaaaagc aatgaaaaaa gtcagcgagt ctagcagtag cgtcatattg agacttgcaa
     7141 actgtaaaac aaggaaggaa agaacaggag agctaacacc tacaacctgc aaaaccagct
     7201 ctaagcgaga attatagatc acaatctcat ctttctccac cacttcagtt atgatagctt
     7261 tattggctgt gcgagaaaag gcaaaagcaa tagcctgcac aatgttagca acaatcaaag
     7321 cgccaatcat ccagctatca ttccttatga aagaaatagc cagacaaaga atcccacaaa
     7381 caagatctgc cgtcattaaa atcttacgac gagaaaaacg gtctgaaata actccgccaa
     7441 agggattgac gagaatagat gtgacgagct cagaaatctg atacattcct aaaactgtct
     7501 gtcctatagt ccccatagaa gccaaccaga cactatttcc ataatcatag agcatatttc
     7561 ccattttatt gatagcccca cggctaatca actgcactgc atagcgattc atattaaagc
     7621 tcctctcaaa ttttgaaact attgtatcaa aaccgaaagg agctttttat tttttccctt
     7681 atttgggaaa attaactttt gacaaatttt tcgtagtgtt cctgataata ggctacttgc
     7741 tctggaagac ctaacacatc aaaaatatgc atggcctctt gcatctgctt acagccttct
     7801 ttacactgtc ctttttgata taaggcaaaa ccttttaaat aatggaaaac attacgctca
     7861 taaagcttaa tacctttgtc aataatcttc tctgtataag cctcaaaata gttggcatta
     7921 taaaaagaag aatgctctaa acaatgctgg taacaattga gggccaaaat caaaactaat
     7981 ctcttatggc gactaatctc ttggtaaaat tcctccctct ccataacttc tctaccaatc
     8041 cgagtgacat agtctacatc gtagaaacta tagaggttac cgaaaagaat caactcatac
     8101 atggtccatt cttctgtttt gaagagataa tctgctacct tatccaaatc atcctgcttc
     8161 atatcataag tcgcatctct ttgacaaatc agaccttgta gcaaaatcca gttcagctca
     8221 aaataaaggg aagtcgtcga actcttagac ttttcaagtt gttctctttg aagcttttga
     8281 aaacctgcaa tatcgtttga atagtaaagt gggataatct gtgccatcat agacacatgt
     8341 tcatgattat gaaaattcct tgccttatcc atgaaatttt cgattgttac atgaatgtta
     8401 tccaaaatct caaagaaacg ggagactgcc aggtcagact ccccaagctc aaagcgagat
     8461 aactgagagg tagagcagga ttcgcctgct gcttccttta aagaataatt tccacttgtt
     8521 cgaaattcac gaaatacttt tccaagatgt tccatcttta cacctgatct gataattctt
     8581 cccactcaag catagcttct tcctgacgat ggctgatttt gtccagctca gcctgtaatt
     8641 ccatgagttt gtcggcatcg tttgtttcca acatttgttc agaaatggct tggctttgac
     8701 tttctagctc ttcaatttca gcttctagac tttcgatttg tcgcatgagt ttgcgaactt
     8761 ctttttgact ttctttctgg gcctgatagt cattgactgg acttgcttcc tttgcttgat
     8821 tgctagttga agcttcctca gtctgactca tttctgctgt tgctttcttc tcaacatagt
     8881 agtcgtaatc tccaaggtag agagttgaac cattctcaga caattccaaa acatgagttg
     8941 ccacacgatt gataaagtaa cgatcatgac tgacaaaaag caaggtacca tcaaagtcaa
     9001 tcaaggcatt ttctagcact tccttactat caatatccaa gtggttggtc ggctcatcca
     9061 gaatcaaaaa gttattgttt tccatagaca atttagctaa aagcaaacga gctttttcgc
     9121 caccagatag catgccgact gattttttaa catcatctcc tgagaaaagg aaggccccaa
     9181 gacggttgcg gatttcaact tctggtgtca gtttgaaatc attccagagt tcatccagca
     9241 ccgtattact tggtgtcagc ttgctttggg tttggtcata gtaaccaacc tcaacattag
     9301 cgccaaagcg cttttcacct ttgataaaag gaatctggtc cacaatagac ttgataaagg
     9361 ttgacttgcc gataccattt ggaccaacga tagcgacagc attcatctta cgaagatcta
     9421 ggttgatagg ttgtgacaaa acttccccgt cataaccaat agcagtattt tcaacagtca
     9481 aaacaacatt gcccgacgtt ttttcagact ggaaggtcat gttggctgct ttcttgccag
     9541 cttcaggctt gtccaaacgc tccatttttt ctagttgttt acggcgagat tgagcacgtt
     9601 tggtcgttga agcacgaact agattgcgat tgacaaagtc ttccagagca gcgatttcct
     9661 tctgttgctt ttcatagttt tttgcctcag tagctagctt ttgctccttc aactcaacaa
     9721 aacgagagta attccccaca tagcgatcca aggaatgctt ggtcaaatct agcgtaattg
     9781 tcgcaacctt gtccaagaaa taacggtcgt ggctgacgat aatgagggca ccgctatagt
     9841 ttaccaagta attctctagc caggagatgg tttcaatatc caagtggtta gttggctcgt
     9901 ccaagaccaa gagattgggc ttttcaagga gcattttagc tagagccaaa cgagtatttt
     9961 gaccaccaga aagctcagca attttcatct gccacataga ctcgtcaaac ttgaatccat
    10021 tcaaaatcgc tcgaatatca gcttcatagg taaagccacc tgcttggcga aaattttcag
    10081 ataagcggtc ataatctgac atcagtttat ccaaatcctc accagacttt tcacccatct
    10141 ccagctccat ctgacgcagt tgtttatctg tccgacgtaa gtcatcaaag acatgaagca
    10201 tctcatcata gatggtattt tcagactcaa aacggctatc ttgagctagg taagacagag
    10261 aaatatcttt tttcttattg atttctccac tagttggctc ctcttctcca actaaaatct
    10321 tcaaaagagt agacttacct gcaccatttt tcccaacaag ggcaatccga tctcgttcat
    10381 caacctgcag gttgatatta tcgaaaagaa cctctcctgc aaaagaacgt tcaattttat
    10441 tagcttgtaa aataatcata caagtagtat agcatgtttc cctaaggcat tcaagataat
    10501 cgtaagtctt ttagtacaac ttttataaca taaaat
//
LOCUS       DBERDK010000065        10343 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.065, whole genome shotgun sequence.
ACCESSION   DBERDK010000065 DBERDK010000000
VERSION     DBERDK010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 10343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10343
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.065"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(385..777)
                     /gene="rpsI"
                     /locus_tag="ACL73L_001652"
     CDS             complement(385..777)
                     /gene="rpsI"
                     /locus_tag="ACL73L_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HGS0772596.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVVGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
     gene            complement(796..1242)
                     /gene="rplM"
                     /locus_tag="ACL73L_001653"
     CDS             complement(796..1242)
                     /gene="rplM"
                     /locus_tag="ACL73L_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HGS0772597.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVVNAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISGLI"
     gene            1599..1916
                     /locus_tag="ACL73L_001654"
     CDS             1599..1916
                     /locus_tag="ACL73L_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HGS0772598.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPRYPPRVLVLDMDGRETILLPEEN
                     DLF"
     gene            complement(1961..2773)
                     /gene="folB"
                     /locus_tag="ACL73L_001655"
     CDS             complement(1961..2773)
                     /gene="folB"
                     /locus_tag="ACL73L_001655"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HGS0772599.1"
                     /translation="MDQLQIKDLEMFAYHGLFPSEKELGQKFVVSAILSYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFESYPLVQEMKLELKKPWA
                     PVHLSLDTCSVTIHRRKQRAFIALGSNMGDKQANLKQAIEKMRARGIYILKESSVLAT
                     EPWGGVEQDSFANQVVEVETWLPAQDLLETLLAIESELGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPILKQPIRNLYDALKK"
     gene            complement(2816..3370)
                     /gene="folE"
                     /locus_tag="ACL73L_001656"
     CDS             complement(2816..3370)
                     /gene="folE"
                     /locus_tag="ACL73L_001656"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HGS0772600.1"
                     /translation="MDTQKIEAAVKMIIEAVGENANREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            complement(3351..4673)
                     /locus_tag="ACL73L_001657"
     CDS             complement(3351..4673)
                     /locus_tag="ACL73L_001657"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HGS0772601.1"
                     /translation="MKEIENNQWIANYRTDQPHFGLERMVELLALRGNPHLKLKVLHI
                     GGTNGKGSTIAFLKKMLEKLGLRVGVFNSPYLIHYTDQISINGESISEARLEALMADY
                     QSLMGGEAVANLQGTTEFEIITALAYDYFASEQVDVAIMEVGMGGLLDSTNVCQPILT
                     GITTIGLDHVALLGDTLEAIAEQKAGIIKQGMPLVTGRIAPEALAVIDRIAEGKDAPR
                     LAYGTDYQVRHQESVVTGEVFDYTSAVRQGRFQTSLLGLYQIENAGMAIALLDTFCQE
                     DGRELASNDFLGQALEETSWPGRLEIVSRDPLMILDGAHNPHAIKALLVTLQERFADY
                     HKEILFTCIKTKALEDMLDLLGAMPDTELTLTHFADSRATDESVLKEAAKSRNLSYQD
                     WHDFLEQNLTDKKEEKQTVRIVTGSLYFLSQVRAYLMERKNENGYTKD"
     gene            complement(4675..5649)
                     /gene="folP"
                     /locus_tag="ACL73L_001658"
     CDS             complement(4675..5649)
                     /gene="folP"
                     /locus_tag="ACL73L_001658"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HGS0772602.1"
                     /translation="MMKILEGVTDMSSKANHAKTVICGIINVTPDSFSDGGQFFALEQ
                     ALQQARKLIAEGASMLDIGGESTRPGSSYVEIEEEIQRVVPVIKAIRKESDVLISIDT
                     WKSQVAEAALAAGADLVNDITGLMGDEKMAHVVAEARAQVVIMFNPVMARPQHPSSLI
                     FPHFGFNQTFTVEELADFEILPIEDLMVAFFERALARAAEAGIAPENILLDPGIGFGL
                     TKKENLLLLRNLDKLHQKGYPIFLGVSRKRFVINILEENGFEVNPETELGFRNRDTAS
                     AHVTSIAARQGVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK"
     gene            complement(5744..6451)
                     /locus_tag="ACL73L_001659"
     CDS             complement(5744..6451)
                     /locus_tag="ACL73L_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HGS0772603.1"
                     /translation="MKEKNMWKELLNRAGWIMVFLLAVLLYQVPLVVTSILTLKEVAL
                     LQSGLIVAGLSIVVLALFIIGARKTQLASFNFSFFRAKDLARLGLSYLVIVGSNILGS
                     ILLQLSNETTTANQSQINDMVQNSSLISSFFLLALLAPICEEILCRGIVPKKIFRGKE
                     NLGFVVGTIVFALLHQPSNLPSLLIYGGMSTVLSWTAYKTQRLEMSILLHMIVNGIAF
                     CLLALVVIMSRTLGISV"
     gene            complement(6504..7922)
                     /locus_tag="ACL73L_001660"
     CDS             complement(6504..7922)
                     /locus_tag="ACL73L_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HGS0772604.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFAMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTLMMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGIISLIITLTNVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYTVVGEGADKAQATIAANSSAVPGLVSFNNPAVLVALAGLAITIFFVIKGIKGGIIL
                     SILTTTVLAIAVGLVDLSSIDFANNHVGAAFEDLKTIFGAALGSEGLGALVSDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNGENHQSAKLDKALYSDLIGTTVGA
                     IAGTSNVTTYVESAAGIGAGGRTGLTALVVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLGSLKNIHWDDMSEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQVK
                     DVHAMIWILDALFILNYISMAL"
     gene            8112..8924
                     /locus_tag="ACL73L_001661"
     CDS             8112..8924
                     /locus_tag="ACL73L_001661"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HGS0772605.1"
                     /translation="MTKKIIAVDLDGTLLNSDSQISDFTKRTIKKVAEKGHQVIITTG
                     RPYRMSKDFYRELGLDTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVQRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPEDQFQPELVTKDPNCILLQTRASD
                     KYALAKEMNAFYQHQLSINTWGGPLNILECTPKGVNKSFALDYLLKIMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLPYADEQISLTNDQDGVAKTLQDLFL"
     gene            9263..10282
                     /gene="adhP"
                     /locus_tag="ACL73L_001662"
     CDS             9263..10282
                     /gene="adhP"
                     /locus_tag="ACL73L_001662"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HGS0772606.1"
                     /translation="MKAVVVNPESTGVAIEEKVLRPLETGEALVEVEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGIGIVKEIAPDVKSLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKV
                     EPGQWVVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLALAKEVGADIVINGLEVE
                     DVAGLIKEKTDGGAHSAVVTAVSKVAFNQAVDSIRAGGRVVAVGLPSEMMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVVPVVQKRPVEDAVAIFDEMEKGQIQGRMV
                     LDFTH"
BASE COUNT         3051 a         2337 c         1998 g         2957 t
ORIGIN      
        1 cttccccatt ctatggaatc ttgcattatc cataatatag tagattgaaa ctagaatagt
       61 acacctctac ttctaaaaca ttgttagaat tcgatttgac tatcctcgat ttgtcctatt
      121 cttatttcac attactataa aaataaggta aacttttcaa aaaagcacga aaaaagcacc
      181 cataaggtaa acttttgagt gctttgaaac attgatataa tcgtattgat taacgttttg
      241 aggcaactga ccttgtcagg ttgcagccac atttgtaagc gactaaagtc gcaacaactg
      301 tgtcaattgc accaatttag taagaaagta taaaaaaaga acaccccgaa aggtgctctg
      361 tataagtata gtaattcttt cgaattaacg tttactaaat tgtgatgctt tacgagcttt
      421 cttaagacct ggtttcttac gttcaacttt acgtgagtca cgtgtaagaa gtcctgcgcg
      481 tttcaatgaa tcgcggaagt ctgggtctac ttgaagaagg gcacgagcga taccgtgacg
      541 gatagctcct gattgaccag cgtatccacc acctacaacg ttaacgaaaa cgtcgtatga
      601 acctacagtt gaagtaactg cgaatggttg gttgatgaca agacgaaggt cagcgtgtgg
      661 gatgtactct tcaacatctt ttttgttaac agtgatttta ccagttcctg gaacaaggcg
      721 aacgcgtgca acagcgtttt tacgacgtcc agtacctgca tattgtgctt gtgacatact
      781 ttattgttcc tttccttaga taagtcctga aatgtcaaga acttctggtt gttgtgcagc
      841 gtgagtgtgc tcagctccaa caaatacttt caacttcata ccttgagcgc gtccaagtgt
      901 attgtgtgga agcatacctt taactgattt ctcaatcaaa cgtactgcat ttttagaacg
      961 aagttcacct gcagagattt gtttcaatcc acctgggtgg tttgagtgag tgtagtagat
     1021 tttatcagtt gcttttttac cagtcaattt aactttttca gcattgacaa caatcacgaa
     1081 gtcacctgta tcagtgtgtg gtgtaaatgt tggtttgttt tttccgcgaa gtacgctagc
     1141 aactactgca gaaagacgtc caagtggtac atcagttgcg tcaactacgt accatttacg
     1201 ttcaacttgg cctggtttag ccataaatgt tgttttgttc atgatttctc ctatatatag
     1261 ttcgattttt gtttacggtg atgggtgttc cttcccatcg aaaagtattt ggaaggttcc
     1321 ggggcctttc aaatggggta aacaataccg cctactatca taccaaattt cacccctaaa
     1381 agtcaataga tatgaaaaat atttttcggt aacttctcaa agtaactgaa agttatataa
     1441 tcaagttaat ttgggaattt acctattaac ttcggtagat ttttgggaat ttttttcctt
     1501 ctttttcctt gtagttaaaa tccttttcaa aaaatcacct tttcttgatt tttaatccta
     1561 tttgctgtat gataagggaa aagaaagggg acagagatat ggcttttacc aatacccaca
     1621 tgcggtctgc tagttttggc attgttacca gcttgcccga tgacatcatt gactcttttt
     1681 ggtatatcat cgatcatttc ttaaaaaatg tctttgaatt ggaagaagaa ctcgagtttc
     1741 aattgcttaa taaccaagga aagattacct tccacttttc aagtcaacac ctccctacag
     1801 ccattgattt tgactttaac caccctttcg accctcgtta tcccccaaga gtactggttt
     1861 tagacatgga cggtagagaa actatcctcc tcccagaaga aaatgaccta ttttaaaaac
     1921 tctagccttc agttgcaagt aactgaaaac tagagttttt ctattttttc aaagcatcat
     1981 acaagttgcg gatcggttgt tttaatatcg gatggataaa atgaggcgca atttcctgta
     2041 aggactcaag gacaaaaagg cgttccgcta tgtaaggatg aggcaatatg aggtcgtctg
     2101 tataaaggat ctggtcctcc acaaagagca agtccaaatc aatcaaacga ggtccccaat
     2161 gcacttctct cacccgtccc agctctgact caatggctaa caaggtttct aacaagtctt
     2221 gtgctggtag ccaggtttcc acctcaacca cttgattggc aaagctatcc tgctccactc
     2281 caccccaagg ctccgtcgct aagacactgg actctttgag aatatagatg ccacgagctc
     2341 gcattttctc aatggcttgt ttcaagtttg cttgtttatc tcccatattg cttcctaggg
     2401 cgataaaggc tcgttgcttg cggcgatgaa tggttaccga gcaagtatct agtgacaaat
     2461 gcaccggtgc ccaaggtttt ttcagttcca acttcatttc ttggacaaga ggataagact
     2521 caaaggtacg ttccaccagt ttataggcta ccgtttcaat caaatcttca ctcgtttcct
     2581 gaaaccaagt cgtccactgc tgacacaatt ctccgtaatg gacagaggct gttaaatcca
     2641 agtctgtagc tgccttggtc atatcatagg ataggatggc tgaaacgaca aatttctgcc
     2701 ccaattcttt ctcactagga aaaagaccat gataggcaaa catttccaaa tccttaatct
     2761 gcagttgatc cataatgatt cctttctaga aaaatccgct taaagcggat tctttttata
     2821 gccccattaa acgataagct tgatcgcgga ggtccttatc tgtttcaaat agaccacgag
     2881 ctactgtcgt caaggttgca gtacctggtt ttctaacacc acgcatactc atacacatat
     2941 gttccgcctc aatgacaaca aaggctcctt tagcacctag atagtccatc aaggcatcgg
     3001 ccacttcgat attcaaacgt tcttgaattt gtggtttttt cgaataaact tcaaccgtac
     3061 gggctagctt agacaagcct gccacacgac catctggaat gtaggcaatg tgcgctctac
     3121 cataaaatgg caagaagtgg tgttcacaca tggtatggaa aaagatatcc ttttctacca
     3181 ccatattatc gtcaataatt tcaaaggatt ttgacaaatg ttcctctgct gtttgaccaa
     3241 gacctgaaaa aatctcttga tacatacggg ctacacgagc aggtgtttcc tgcaagccct
     3301 cgcgattagc gttctctcct acagcctcga taatcatttt tacagctgct tcaatctttt
     3361 gtgtatccat tctcgttctt cctctccatc agataggccc tcacttggct caagaaatac
     3421 aaggaacctg tgacaatcct aactgtttgt ttctcttctt ttttatctgt caaattctgc
     3481 tctagaaaat catgccaatc ttggtagctg agatttctag acttagctgc ctctttcagc
     3541 acgctttcat ccgtcgcccg actatccgca aaatgtgtta gagtaagctc ggtatctggc
     3601 atggctccca gcaagtccaa catatcctcc aaggctttgg ttttgataca agtgaagagg
     3661 atttccttat gataatccgc aaaacgttct tgcaaggtta ccaacaaggc cttgatagca
     3721 tggggattat gggctccatc caaaatcatc aagggatctc ttgacacgat ttccaaacgc
     3781 cctggccaac ttgtttcttc caaggcttga ccaagaaagt cattgcttgc tagctctcga
     3841 ccatcttctt gacaaaaagt atcaagtaaa gctatggcca tcccagcatt ctctatttgg
     3901 tacaaaccaa gcaggctagt ctggaagcga ccttgtctga cagcacttgt atagtcaaag
     3961 acttcccctg tcaccacact ttcttgatga cgaacctgat aatctgtccc gtaggcaagt
     4021 ctcggcgcat ctttcccttc cgcaatgcgg tcaatcacag ccaaggcttc tggagcaata
     4081 cgccctgtta ccaagggcat cccttgtttg ataatacctg ccttctgctc tgctatggcc
     4141 tccaaggtgt caccaagtag ggccacatga tccaagccaa tagttgtaat tcctgtcaaa
     4201 atgggctgac agacattggt actatccaaa agtccaccca tgccaacttc catgatggcc
     4261 acatctactt gctctgaggc aaagtagtca taggccaggg ctgtgataat ctcaaactcg
     4321 gttgtgccct gtaaattggc gaccgcttct ccccccatca aagactgata gtctgccatg
     4381 agagcttcta gcctcgcttc tgagatcgat tccccattga tgctaatctg gtctgtgtaa
     4441 tgaatgagat agggcgagtt aaacacgcca actctcagcc ctagcttttc tagcatcttt
     4501 ttcaaaaaag caatagtcga gcccttgccg ttagtccctc cgatatggag gaccttgagt
     4561 ttgagatggg gattgccacg caaagctaac agttccacca ttcgttccaa accaaaatgc
     4621 ggttgatccg tccggtagtt agcaatccac tgattgtttt caatttcttt catcttattt
     4681 atattgtttt aaatctaaat tttccgcttc atcagccaga cgaatggcag aggcaatttc
     4741 aactgccatc ctgtgactag ctacgtcatg cacgcgcacc acttctacac cctgtctcgc
     4801 agcgatacta gttacatgag ccgaagccgt gtcccgattt cggaaaccaa gctctgtctc
     4861 aggattgact tcaaaaccat tctcctctag gatattgatg acaaatcgct tgcgcgaaac
     4921 tccgagaaag attggatagc ctttctgatg tagtttatcc aggttccgta aaagaagcag
     4981 attttctttc ttggtcagac caaagccaat tcctggatcc aacaggatat tttctggtgc
     5041 aataccagct tctgccgctc tcgctagtgc tcgttcaaag aaagccacca tcaagtcttc
     5101 gattggcaat atttcaaagt cagctaactc ttctactgta aaagtttgat taaatccaaa
     5161 atgagggaat atgagcgagc tagggtgttg aggtcgcgcc ataactggat taaacatgat
     5221 gaccacttgc gctctcgctt cagctaccac atgagccatt ttttcatcgc ccatgagacc
     5281 agtgatatca ttgactagat cggcaccagc agccaaagca gcctctgcta cttgactctt
     5341 ccaagtatca atagagatga ggacatcact ttccttgcga atcgctttga tcactggaac
     5401 aacacgctgg atttcctctt ctatctcaac atagctactg cccggccgag tcgattctcc
     5461 gccgatatcg agcatactgg ctccttctgc tatcaattta cgagcctgct ggagtgcctg
     5521 ctcaagagca aaaaattgac caccgtccga aaaggagtct ggggttacat tgataattcc
     5581 gcaaataact gtctttgcat gattggcttt acttgacata tcggtcactc cctcaaggat
     5641 tttcatcata ttatttttct attttaccat aaaaagaaaa agatggacac gattgcattc
     5701 atctttccct agtagaaaga ggtcggttcc tacataaaaa cttttacaca gaaattccta
     5761 atgtccgact cataatcacc acaagagcca acaaacagaa agcaatccca ttaacaatca
     5821 tgtgaagcaa gatcgacatt tccaaacgtt gggtcttgta ggccgtccaa gatagaactg
     5881 tcgacatacc tccataaatc aataaagaag gtaaattact tggttgatgc aataaagcaa
     5941 acacaatcgt accgactaca aatcccaagt tctccttgcc tcggaaaatc tttttaggaa
     6001 caatcccacg gcacaagatt tcctcacaaa tcggagcaag caaggctagc aagaagaaac
     6061 tggaaatcaa cgaactattt tgaaccatat cgttaatctg agactggtta gctgttgtcg
     6121 tctcatttga cagttgcaac aagatagaac caagtatatt tgacccgaca ataactaaat
     6181 aactcaagcc caaacgtgcc aaatctttag ctctaaaaaa agaaaaatta aaacttgcca
     6241 attgcgtttt acgagctcca ataataaata gagccagaac cacaattgaa aggccagcaa
     6301 ctatcagccc tgactgtagc agggctactt cttttaaagt caaaatagag gtaaccacta
     6361 ggggaacctg atataaaagg acggcaagta aaaagaccat aatccagcct gcacgattca
     6421 acaattcttt ccacatattt ttctctttca tcacctatct cctttgtatt aaaaaagggg
     6481 ggaaatcccc ctgggtcatt ctattataag gccatgctga tgtagttaag gataaacaag
     6541 gcatccaaaa tccaaatcat ggcatgaaca tctttaactt gacctttaac aagcttagtc
     6601 aaagtgtaag tcaagaaacc aactgcaatc ccttgagtga tagagtagct gaatcccata
     6661 aagatagatg tgaagaaggc aggaactgct tcagacatat catcccaatg gatatttttc
     6721 aagctaccaa gcatcataat cccaacgata atcaagattg gagctgtagc cgctgttggt
     6781 acgatcgcta gaagtgggct aaagaagctt gaaatcgcaa aacagatagc tacaaccaag
     6841 gctgtcaaac cagtacgtcc acctgcaccg ataccagcag cagactcaac ataagtcgtt
     6901 acgtttgaag tacctgcaat agcaccgact gtcgttccaa tcaaatcaga gtaaagagcc
     6961 ttatccaatt tggctgattg gtgattttca ccatttgtcg ctacgatacc aactttttca
     7021 cctgtaccga tcaaggtacc aattgtgtca aaaatatctg tcaatgagaa ggcaagaatg
     7081 gccatcagag tttcaggcaa gcgagctgta tctgaaacca aagctcccaa accttctgaa
     7141 ccaagagctg caccaaagat tgtcttcaaa tcttcaaaag ctgcaccaac atggttatta
     7201 gcaaaatcga tactagacaa atcaaccaaa ccaactgcaa tagcaagaac agttgttgtc
     7261 aagatagaga gaataattcc ccctttaatc cctttgatga caaagaagat agtaatggca
     7321 agtcctgcaa gagccactaa aacagctgga ttattaaagc tgaccaatcc tggaactgct
     7381 gaagagtttg ctgcaatcgt tgcttgagct ttgtcagccc cttctcctac aacagtatag
     7441 ttgcctggat caatcgtgaa tttcaaaagt ccagcattct taatccctac ataggcaagg
     7501 aagacaccga taccagctga aatagctgag cgaagagcat tgggaatcga ttcaatgatc
     7561 attttacgaa catttgtcaa ggtaataatc aatgaaataa tcccacagat gaagaccata
     7621 gctagggctt cttgccaaga ataaccaagc ccgaatacaa ctgtaaaggt aaagaaggca
     7681 ttgagtccca tacctggcgc ttgggcataa ggtaagttag cataaaaagc catcatcaag
     7741 gtacccgcta ctgcaccaat aatcgtcgct aggaagacgc cctgagcagg cattcctgtt
     7801 tgtgaaagta tttgtgggtt tacaaagaga atatagctca ttgcaaagaa agttgttaaa
     7861 ccagcgagaa cctctgtacg aacgtctgta ccgttctctt ttagtttaaa taatttgtcc
     7921 atcatcaatc tccttttaat ttttacgaac aataatagaa ttatatcatt tatcattcac
     7981 tttttcaatg tctttgtttg atttgtttaa gaaattaatc caatttcatt ttttgtttgg
     8041 aatctgcttt tctgcctgct ttctgttata atagaagaca tcttatctga aaacacacta
     8101 gaaaggtcca tatgacgaaa aaaattattg cagttgacct ggatggaacc ctgctcaact
     8161 cagacagtca aatttctgac tttaccaaaa gaaccatcaa aaaagttgct gaaaaaggcc
     8221 atcaggttat tattacgaca ggtcgccctt accgtatgtc aaaagatttt taccgtgaac
     8281 tgggcttaga cactcctatg attaacttca acggatccct tactcattta ccagaccaag
     8341 tttgggattt tgaaaagtgt ttgactgtag acaaaaaata tctgctagat atggttcaac
     8401 gttcagagga cattcaagcc gattttatcg ctggagaata tcgtaaaaaa ttctacatta
     8461 caaatcccaa tgaagaaatt gccaatccca aactatttgg tgtagaagct ttccagcctg
     8521 aagatcaatt ccagcctgaa ttggtgacca aggaccctaa ctgtatcctc ttgcagacca
     8581 gagccagtga caaatatgcc ttggcaaaag aaatgaacgc cttctaccag catcaacttt
     8641 ctatcaatac ctggggaggt ccgctcaata tccttgaatg taccccaaaa ggtgtcaaca
     8701 agtcctttgc tttggactac ttgctcaaga taatgaatcg tgacaaaaaa gatttgattg
     8761 cctttggaga tgaacacaat gataccgaaa tgctcgcttt tgctgggaag ggttatgcca
     8821 tgaaaaatgc caatccagag ctactccctt atgcagatga gcaaatttcc cttaccaacg
     8881 accaagatgg ggttgccaaa accctacaag acttattctt ataacctata ctgatactca
     8941 atgagaatca aagagcaaac taggaagcta gccgtaggtt gctcaaagca ctgctttgag
     9001 gttgtagatg aagctgacgt ggtttgaaga gattttcgaa gagtatgacc agcttgcaag
     9061 ctggtctttg tgctcttttc acagtctcat caaccagtta atcgattgtt ctactttttg
     9121 tctccaaaac cttggaaaag gctttctttt gtgatatact tcacatataa atacaagaga
     9181 gctcgtcaca ctcgactctg ttgacaaaaa atcaatccag tcccgttgtc cctgttctcg
     9241 gtcaatatca aggaggatag ctatgaaagc tgttgttgta aatccagaaa gcactggtgt
     9301 tgctattgaa gaaaaagtac tccgtccact tgaaactggg gaagcacttg tagaagttga
     9361 atactgtggt gtttgccaca ccgacctcca cgttgctcat ggtgactttg gtcaagtccc
     9421 aggacgtgtt cttgggcacg aaggtatcgg tatcgttaaa gagattgctc cagatgtgaa
     9481 aagccttaaa gtcggtgacc gcgtcagcgt tgcttggttc tttgaaggat gtggcacttg
     9541 cgaatactgt acaactggtc gcgaaaccct ttgccgtaca gtgaaaaatg ctggctactc
     9601 agtagacggt ggtatggctg aacaatgtat cgtaactgct gactatgctg tcaaagttcc
     9661 tgacggactt gatccagccc aagcttcttc tatcacatgt gctggagtaa caacctataa
     9721 agctatcaaa gaagcaaaag ttgaaccagg ccaatgggtt gttctttacg gtgctggtgg
     9781 acttggtaac ctcgctgttc aatacgctaa aaaagtattc aatgctcatg ttatcgcagt
     9841 cgatatcaac aatgacaaac ttgcccttgc aaaagaagta ggcgctgaca ttgtgattaa
     9901 cggcctcgaa gttgaagatg tagctggact cattaaagaa aaaactgatg gaggagctca
     9961 ttcagctgtc gtaactgctg tgtctaaagt tgccttcaac caggctgttg actccattcg
    10021 tgctggtggt cgcgtcgtcg ctgttggtct tccttctgaa atgatggaac tcagcatcgt
    10081 taaaacagtc ctcgatggaa tccaagtcat cggttctctt gtcggaactc gtaaagactt
    10141 agaagaagcc ttccaatttg gtgcagaagg tctggtagtc ccagttgttc aaaaacgtcc
    10201 agtagaagat gctgttgcca ttttcgacga aatggaaaaa ggccaaatcc aaggacgtat
    10261 ggtactcgac ttcacccact aatctaatag aaacctattt taaaagttgg aagcttcttc
    10321 caactttttt atattaaatt tta
//
LOCUS       DBERDK010000066        10286 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.066, whole genome shotgun sequence.
ACCESSION   DBERDK010000066 DBERDK010000000
VERSION     DBERDK010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 10286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10286
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.066"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            85..552
                     /gene="tadA"
                     /locus_tag="ACL73L_001663"
     CDS             85..552
                     /gene="tadA"
                     /locus_tag="ACL73L_001663"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HGS0772607.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSEESWRLLDCTLFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGATGSLYDILTDERLNHRVEVETGILEDECAAIMQDFFRNRRKK"
     gene            585..682
                     /gene="ffs"
                     /locus_tag="ACL73L_001664"
     ncRNA           585..682
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACL73L_001664"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            739..1182
                     /locus_tag="ACL73L_001665"
     CDS             739..1182
                     /locus_tag="ACL73L_001665"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HGS0772608.1"
                     /translation="MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH"
     gene            1184..1699
                     /locus_tag="ACL73L_001666"
     CDS             1184..1699
                     /locus_tag="ACL73L_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HGS0772609.1"
                     /translation="MKIIFVRHGEPDYSELEERSYIGFGINLAPLSEMGRQQVQKLSK
                     NPLLSSAEIIVSSAVTRALETASYVVCATGLPLRVEPLLHEWQVYKTGIENFETARRL
                     FLENKGELLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMLMRQFVPNEKID
                     FCQVIECELEI"
     gene            1713..3074
                     /gene="radA"
                     /locus_tag="ACL73L_001667"
     CDS             1713..3074
                     /gene="radA"
                     /locus_tag="ACL73L_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016893792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HGS0772610.1"
                     /translation="MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKN
                     ARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
                     RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLI
                     MAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGE
                     LGLTGEIRRVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKK
                     VFA"
     gene            3147..3644
                     /locus_tag="ACL73L_001668"
     CDS             3147..3644
                     /locus_tag="ACL73L_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class carbonic anhydrase"
                     /protein_id="HGS0772611.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENEPF
                     QEYLKEELGVDVSDQDFLPFQDIEESVREDMQLLIESPLIPDDVIISGAIYNVDTGSM
                     TVVEL"
     gene            3669..4338
                     /locus_tag="ACL73L_001669"
                     /pseudo
     CDS             3669..4338
                     /locus_tag="ACL73L_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
     gene            4483..5451
                     /locus_tag="ACL73L_001670"
     CDS             4483..5451
                     /locus_tag="ACL73L_001670"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HGS0772612.1"
                     /translation="MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALAEAGAVEVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
     gene            5569..6514
                     /locus_tag="ACL73L_001671"
                     /pseudo
     CDS             5569..6514
                     /locus_tag="ACL73L_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175244.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
     gene            7012..9645
                     /gene="polA"
                     /locus_tag="ACL73L_001672"
     CDS             7012..9645
                     /gene="polA"
                     /locus_tag="ACL73L_001672"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HGS0772613.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNAAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELLDY
                     MGIRHYELAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEHGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGK
                     FYDEMGFKQLKQALNVSSADVSESLDFTIVDQISQDMLSEESIFHFELFGENYHTDNL
                     VGFAWSCGDKLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     AVLVETEPILLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIVVKKETLLEMQAENEL
                     VIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYMQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIH
                     TSTAMRVFGIERPDNVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPGIKNYMDEVVREARDKGYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEMKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
     gene            9730..10167
                     /locus_tag="ACL73L_001673"
     CDS             9730..10167
                     /locus_tag="ACL73L_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HGS0772614.1"
                     /translation="MSQEVINPSDGVIRQYLATSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKAAGGEILGEKAYASLAEIPFPVDIVNVYRRSEFLPDVARDFLKADA
                     KIFWAQLGLESLEAKEILRDGGCDDIVMNRCIKREHTRLIEEA"
BASE COUNT         2854 a         1780 c         2563 g         3089 t
ORIGIN      
        1 tagagtcacc gcattcggtg gctttttttg tgttgggatt catgatataa taataaaatc
       61 gataagtagg aaaagagaaa agagatgtat tatacgcttg aagaaaaaga agtctttatg
      121 agggaggctt tgagagaggc tgagattgct cttgaacacg atgaaattcc aattggttgt
      181 gtgattgtca aagatgggga aatcattggt cgtgggcata atgcgcgtga ggaattacag
      241 cgagcggtta tgcatgcgga aattatggct atagaggatg cgaacttgag tgaggagagc
      301 tggcgcttgc tggattgcac actttttgtg accattgaac cttgtgtcat gtgtagtggg
      361 gcgattgggc ttgcccgcat tccaaatgtg gtctatgggg ctaaaaacca gaaatttggc
      421 gctactggaa gtttgtacga tatcttgaca gatgagcgtc ttaaccatcg tgtagaggtt
      481 gaaacgggaa ttttggaaga tgaatgtgca gctatcatgc aggacttttt tagaaataga
      541 cggaaaaaat aattttgctt ttaaaatgaa taggaatgtg atataataaa tagtggagca
      601 acagttctgc gtgaagcggg tcaggggagg aatccagcag ccctaagcga tttgaattgt
      661 gtgctctttt tttcgtgctt tttccgaata aataagatag aataatctag aataaatgat
      721 aatagaaaag agaaaattat gaaaattcgt ggttttgaat tggtttcgag ttttacagat
      781 gaaaatttat tgcccaagcg tgagacagcg catgcggctg gttacgactt aaaggttgct
      841 gtgcgtacag ttgttgcgcc aggagagatt gtcttggttc cgacaggggt taaggcttat
      901 atgcagccga ctgaggttct ctacctctat gatcgttctt caaatcctcg taagaagggc
      961 ttggttttaa ttaactcagt tggggtcatt gatggggatt attatggaaa tcctggaaat
     1021 gaagggcata tttttgcgca gatgaagaat atcacagacc aagaggttgt tcttgaagtt
     1081 ggggagcgta ttgtccaggc tgtttttgct actttcttaa ttgcagatgg agatgcagct
     1141 gatggcgttc gaactggtgg atttggatcg acagggcact agaatgaaga ttatctttgt
     1201 acgtcatggg gagccggatt acagtgagtt agaggagcgt tcttatatag gatttgggat
     1261 aaatttggca cccttgtctg agatgggacg gcagcaagtc cagaaattga gcaaaaatcc
     1321 tttactctcg tcagctgaaa taatcgtatc ttctgcagtc acaagagctt tagaaacggc
     1381 ttcgtatgtg gtctgtgcta cgggtcttcc tttaagagta gagcctttat tacatgaatg
     1441 gcaggtctat aaaacaggaa tagaaaactt tgaaacagct agaagactgt ttttagaaaa
     1501 caagggggag ttgcttccta atagtcctat tcaatatgag acagctacgg aaatgaagtc
     1561 tcggtttcta gaatgtatgt ctaagtatcg agaacatcag actgtggtag ttgttgctca
     1621 tcgaatgctc atgcgccagt ttgtgccaaa tgagaagatt gatttttgcc aagtgattga
     1681 gtgtgagtta gagatataga aagaggtttg tcatcgcaaa gaaaaaagcg acatttgtat
     1741 gtcaaaattg tgggtataat tcccctaaat atctgggacg ttgccccaac tgtgggtctt
     1801 ggtcttcttt tgtggaagag gttgaggttg ccgaggtcaa gaatgcgcgt gtgtccttga
     1861 caggtgagaa aaccaagccc atgaaactag ctgaggtgac ttccatcaat gtcaatcgaa
     1921 ccaagacgga gatggaggaa ttcaaccgtg tgcttggagg cggagtggta ccaggaagtc
     1981 tcgtcctcat cggtggggat cctgggattg ggaaatcaac tcttctccta caagtctcaa
     2041 cccagttgtc ccaagtgggg acagttctct atgtcagtgg ggaggagtct gcccagcaga
     2101 ttaaactacg tgcagagcgc ttaggtgata ttgatagtga gttttatctc tatgcagaga
     2161 ccaatatgca gagtgttcgt gcagaagtgg agcgtatcca gccagacttt ctcattattg
     2221 attccatcca gaccatcatg tctcctgaga tttcaggggt gcaggggtct gtttctcagg
     2281 tgcgtgaagt gaccgctgaa ctcatgcagt tggccaagac caataacatt gccatcttta
     2341 tcgtaggtca tgtgaccaaa gaaggaacct tggctgggcc tcgtatgttg gagcatatgg
     2401 tggatacggt gctttacttt gaaggggagc gtcaccacac ctttcgtatt ttgagagcgg
     2461 tcaaaaatcg ttttggttcc actaatgaga ttgggatttt tgagatgcag tcgggcggct
     2521 tggttgaggt actcaatccg agtcaagttt tcctagaaga gcgtttggat ggggcgactg
     2581 gttcctccat cgttgtaacc atggaaggga cgcgtccgat tttggcggag gttcaggctt
     2641 tggtaacacc gaccatgttt ggaaatgcca agcgtactac gacaggactt gattttaacc
     2701 gtgctagctt gattatggct gttttggaaa aacgggcagg gcttctcttg caaaatcagg
     2761 atgcctatct caaatctgct ggtggtgtta aattggatga acctgcgatt gacttggctg
     2821 ttgcagttgc tattgcttcg agctacaaag acaagccaac taatcctcag gaatgttttg
     2881 tcggagaact gggcttgaca ggagagattc ggcgcgtgaa tcgtattgag caacgcatca
     2941 acgaagctgc taaactgggc tttactaaga tttatgtacc taagaattcc ttgacaggaa
     3001 tcactctgcc taaggaaatt caggtcattg gcgtgacaac gattcaggaa gtcttgaaaa
     3061 aggtctttgc ataatccgtg acaaattctc ttaaaaatga taagatagga gaaatatttg
     3121 actatcaaat tttcaaggag ggaatcgtgt cgtattttga acagtttatg caagccaatc
     3181 aggcttatgt tgccctacat gggcagttaa atctgccact taaacccaaa acaagagtag
     3241 ctattgtgac ctgtatggac tctcgtctgc acgttgcgca agctctgggc ttggcacttg
     3301 gggatgctca tatcttgcgg aatgcaggtg gtcgagtgac tgaagacatg attcgttcgc
     3361 tagttatttc ccagcaacaa atggggacaa gagagattgt ggtattgcac catacagact
     3421 gtggtgctca gacctttgaa aatgaacctt ttcaggagta tttaaaagag gaattaggtg
     3481 tagatgtgtc agaccaggac ttcttgccct tccaagatat agaagagagt gtacgcgagg
     3541 atatgcaact gcttatcgag tctcccctaa taccagacga tgtcattatc tctggtgcca
     3601 tttacaatgt tgatacagga agtatgacag tcgtagaatt ataaatactt catttagaaa
     3661 gaaagtgtat gaagaaaagc agtattttat ttatttttat tttattgcta tgtattggtt
     3721 tacagtatga aaccatctac tatacggacg gttcgaggtc aggtgcggaa tatggactaa
     3781 tgggagtttc tatctttcta gctctctttt acatgattcc ggctctttat tttctcttcc
     3841 gtattgggaa aaaatgggaa ttgccaaaga aggttttgat tctgtcttta ttgggaggga
     3901 tgttcctttc aggctggttg tctagttttg ctaatactta tatccatgat ttactggggg
     3961 ttcttttccc agatagtaca tttttaaatg cctttgaaag tgctattgtg gctcctttgg
     4021 tagaagaacc cttgaaatta ttgccacttg tttttgtttt ggctttgatt cctgtgcgaa
     4081 aattaaaatt tttgttttta cttggaattg cctctcactg gactttttca ggtctagctg
     4141 tagtaggtgt ttacttgctt tacagagcct ataaaggaca gaaggttggc aagaaacagg
     4201 gtcttattgt ttaactctcc ttttgtggag ttggaaacag agttgccttt agcgattcca
     4261 gtggttacgg ctattgctct ctatggttat tatcatgctt attgctttgt tgagaaacac
     4321 aatgagttga tgacctagaa tatttttcaa aagaatgatg caagggtaca aatatggtgc
     4381 ccttctttta tttttgattg aaaaatagtg caaaaagcgc tacaatggta gatggaaaat
     4441 cttgtgaaaa gcacaagcga tacatatata ccggaggaaa tcatgtcttt ttctgattta
     4501 aagctgtttg ccctttcttc taataaagaa ttggcagaac gtgtggcgca ggagattggg
     4561 atagagttgg ggaaatcaag tgttcgccaa ttttcagatg gagagattca ggtcaacatt
     4621 gaagaatcaa tccgtgggaa acacgtcttt atcctacaat caactagttc gcctgtaaat
     4681 gacaatctgc ttgaaatttt gattatggta gatgctttga agcgtgcgag tgcagaatct
     4741 gtcaatgttg tcatgcctta ctatgggtat gcacgtcagg atagaaaggc gagagcgcgt
     4801 gagccaatca cttcaaaact tgtcgcaaat atgcttgaag tagctggagt ggatcgttta
     4861 ttgaccatcg acttgcatgc tgcgcaaatt caaggattct tcgatattcc tgtggatcat
     4921 ttgatgggtg ctcctctaat tgcagattat tttgagcgtc gtggtatggt tggttctgac
     4981 tatgtggttg tcagcccaga ccatggaggg gtgactcgtg ctcgtaagtt ggcagaattt
     5041 ttgaaaacat ctatcgctat tattgataaa cgtcgtagcg ttgataagat gaatactagt
     5101 gaagttatga acatcatcgg taaggttgaa ggcaagactt gtatcttgat tgatgatatg
     5161 attgataccg ctggaacgat ttgtcatgcg gcagatgctc ttgcggaagc tggtgctgtt
     5221 gaagtctatg caagctgtac gcacccagtt ctttctggtc ctgctacgga caatatccaa
     5281 aaatcagcta ttaagaaatt ggttgttttg gataccatct atctgccaga agagcgtttg
     5341 attgataaga ttgagcagat ttcaatcgct catctcctag gggatgctat cgtacgtatt
     5401 catgaaaaac gaccactttc tccacttttc gatattgaga aaaaaattta atgaccaagc
     5461 ctgagatgat tctcaggttt tttcgtcttt tttccgaata aatagatagt agcggtgaat
     5521 ctagtaaagc tagatttaaa actgtgatat aataaaaaga gaagaaatat gactgtacgt
     5581 catccgggca ttagcccaac caatgacttg gtagctaaga aaatctttag caatccagaa
     5641 atcacttgtc aatttattcg cgatatgctg gatttgccag ccaaaaatgt gaccattttg
     5701 gagggtagca atattcacgt attactctcc atgccttact cggtgcagga tttttatacc
     5761 agtatagacg tcttggggga gttggataac agtactcaag taattattga gattcaagtc
     5821 catcatcaga atttttcatc aatcgcttgt gggcttacct gtgcagtcag gttaatcaaa
     5881 atcttgaaaa aattcgtcag cgagaaggtg atactcacta gagctacaaa cacatcgctc
     5941 ctgtttacgc cattgctatc gtggatagta attatttctc agatgacctg gcttttcata
     6001 gctttagtat gcgcgaagac acaacaggtg aggtattggc gattaccaac aatggacagg
     6061 aaaaccatct ggttaagatg gcattcttgg aattaaaaaa tacagagaaa ccagcaaaga
     6121 caaggttcgc aagccatggt tggagttttt cggcaacaag ccctttaccc agcaaccgca
     6181 acgagccatt acccaagcaa atcaactgct ggactacaag agctggtccg aagaggacag
     6241 gaaaatgttt agtcaactac atatgcgaga agaacaagtc ttgttagcac aggactatgc
     6301 cttggaaact gctagggctg aaggccttga acaaggactg gagcgtggta ttgaacgtgg
     6361 tcgtgcagag ggtcgtgaac aaggacgaga agaaggcctt gaagagggat taaaagtagg
     6421 tttagtaaat ctagtacgtc aaggtcttct aacacctgag gttgcgagtg agcaattagg
     6481 catgaccgtg gctgagtttg agtccttgct atgagttcat actttatata gtagaaagtt
     6541 gatgggatgg caagtttatt atcaaaatac ttgtcatttt tgcttgacat ttctatcttt
     6601 ttataagata gtattagcga agtaagttat gtctcgctat tcatacagtt tggatagtcg
     6661 caagattgtc tttgagattt cctgttttaa agagaaaaaa gcctctttga cgctattttt
     6721 tcatcttttt gaaagttcta taatgaagtt agccacccag ccctcctttt cctccttcta
     6781 tagtgagttg aaataagatg tgaataactc tatcaggaaa gtcaaattaa tttatagaaa
     6841 tattttagca gtcaaggtgt actattatag attcaatata ttatagcaca tttttaaatc
     6901 taggtttact aaattctctg gctctatcta tttattcgga aaaagtttga aaaatacttg
     6961 ctctagctct tcccaatggt attttttgat tctttccttt ataatgggtg tatggataag
     7021 aaaaaattat tattgattga tgggtcttct gtagcttttc gggcgttttt tgcgctgtat
     7081 cagcagttgg accgttttaa gaatgcggct ggtttgcata ccaatgcgat ttatggtttt
     7141 cagttgatgt tgagtcattt gttggagcgg gttgagccga gtcatatttt ggtggctttt
     7201 gatgcgggaa agacgacctt ccggacagag atgtatgcgg actataaggg tggtcgggcc
     7261 aagactcctg atgagtttcg tgagcaattt cctttcattc gtgagttgct ggattatatg
     7321 gggattcgtc actatgagct ggctcagtat gaggcggatg acatcattgg gacgctggat
     7381 aagctagcag agcaggatgg ttttgatatt accattgtca gtggggacaa ggatttgatt
     7441 cagctgacgg atgagcatac ggtggttgaa atttccaaga aaggtgtggc tgagtttgag
     7501 gcctttacgc cagattacct catggaagaa atgggcctca caccagctca gtttatcgat
     7561 ctcaaggcgc tcatgggtga taagtcggat aatatccctg gggtgaccaa agtcggtgaa
     7621 aagacgggta ttaagctctt gctggagcat ggttcgcttg aggggattta tgaaaatatt
     7681 gatggaatga agacttctaa gatgaaggaa aatctcatca atgacaagga acaggccttt
     7741 ttgtctaaaa cactagcgac cattgatacc aaggcaccga ttgcgattgg tttagaggac
     7801 ttggtctata gtggtccaga tgttgaaaat cttgggaaat tttacgatga gatgggcttc
     7861 aaacagctca agcaggcttt aaatgtgtcg tcagctgatg tgtctgagag tttggatttt
     7921 actattgttg accaaatcag tcaagatatg ctgagtgaag agtctatctt ccactttgag
     7981 ctttttggtg agaattacca tacggataat ttggttggat ttgcctggtc ttgtggggat
     8041 aagctctatg ccacagacaa gcttgagctg ttgcaagacc cgattttcaa ggatttctta
     8101 gaaaaaacat ctctgagagt ttatgacttt aagaaggtta aagttctttt gcaacgtttt
     8161 ggtgtggatt tgcaggcgcc tgcttttgac atccgtttgg ctaaatacct cctttcgact
     8221 gtggaggaca atgaaattgc gaccatcgct agtctttatg gtcagactta cttggttgat
     8281 gatgaaactt tctacggtaa gggtgttaaa aaggccattc ctgaacgtga gaaattcttg
     8341 gaacatttag cttgtaaact tgctgttttg gtagaaacag agcctatttt acttgaaaaa
     8401 ctcagcgaaa atgggcaatt agagcttctt tatgatatgg agcaacctct ggcttttgtt
     8461 cttgccaaga tggaaattgc tgggattgtg gtcaagaaag agaccttgct tgagatgcag
     8521 gctgaaaatg agcttgtcat tgaaaaactg actcaagaga tttacgagct ggctggtgag
     8581 gagtttaatg tcaactcgcc taagcagttg ggcgtgcttc tctttgagaa attgggactt
     8641 cctctagaat acactaagaa aaccaagaca ggttattcga cagcagtgga tgtgttggag
     8701 cgtctcgctc ctattgctcc gattgttaag aaaatcctgg attaccgtca aattgctaag
     8761 attcaatcta cttatgtaat tggcttgcag gactggattt tggctgatgg aaagattcat
     8821 actcgctata tgcaggattt gacccagacc gggcgtttgt ctagtgtgga tccaaacttg
     8881 caaaatattc ctgcccgatt ggaacaaggg cgcttgattc ggaaggcttt tgtgccagag
     8941 tgggaggata gtgtgctact cagctctgac tattcacaga ttgaattgcg cgttttggcg
     9001 catatttcta aggatgagca cttgattaag gccttccaag agggggcaga tatccatact
     9061 tcgacagcca tgcgggtctt tggcattgag cgtcctgata atgtgactgc aaatgaccgt
     9121 cgcaatgcca aggcagttaa ctttggagtg gtttatggga tttcagactt tggcttgtct
     9181 aataatttgg gaattagtcg taaggaagcc aaagcctaca ttgataccta ctttgaacgt
     9241 tttccaggta ttaaaaacta catggatgaa gtggtgcggg aggcgcgtga taagggctat
     9301 gtagagaccc tctttaagcg tcgccgtgag ttgccagata tcaattcgcg caacttcaat
     9361 attcgtggtt ttgcggagcg aactgctatc aactcaccta tccagggttc ggcagcagat
     9421 attctcaaga ttgccatgat tcagctggat aaagccttgg ttgcaggtgg ttatcagact
     9481 aagatgctgt tacaagtgca cgatgaaatc gtccttgaag tgcctaaatc tgaattggta
     9541 gagatgaaaa aattggtgaa acaaaccatg gaagaagcca ttcaactcag tgttcctctt
     9601 atcgcagatg agaatgaagg ggcaacctgg tacgaggcta aataaaaagg gggctagtcc
     9661 tccttttttg tagtagaatt ctgcaaacct tttcaaaatg tgctatactg atgaaaaagg
     9721 aggatttcta tgagtcaaga agttatcaat ccaagtgatg gcgtgattcg tcagtatctc
     9781 gcaacgagta aaacccttgc tgtggtgggt ttgtctgacc gtgaagaaac aaccagcaat
     9841 cgagtgacaa aggaaatgca ggctcggggc tataaaatca tcccagtcaa tcccaaggcg
     9901 gcaggtggcg aaatcttggg tgaaaaggct tatgctagcc tcgctgaaat tccttttcct
     9961 gtagatattg tcaatgttta tcgtcgcagt gaatttctgc ccgatgtggc gcgtgatttt
    10021 ctcaaagctg atgccaagat tttttgggca cagctaggac ttgaaagttt agaagcgaaa
    10081 gagattttgc gtgatggtgg atgtgatgat atcgtgatga atcgctgtat caagagagaa
    10141 catactcgct tgattgagga agcataagaa aaaggtagct gatgggctac cttttgtgtt
    10201 atactcaatg aaaatcaaag agcacactag caaactagga aactagccgc gggctgctca
    10261 aaacaccgtt ttgaggttgt agatac
//
LOCUS       DBERDK010000067        10103 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.067, whole genome shotgun sequence.
ACCESSION   DBERDK010000067 DBERDK010000000
VERSION     DBERDK010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 10103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10103
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.067"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            443..1804
                     /gene="trpE"
                     /locus_tag="ACL73L_001674"
     CDS             443..1804
                     /gene="trpE"
                     /locus_tag="ACL73L_001674"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HGS0772615.1"
                     /translation="MERIIHGDVLSPILAYMRLKGQHKVILESISRDKETARFSILAY
                     NPVFEIKFENGVLYQNGQVIDRDPLDFLYEVIHKSQHHSELPFGGGAIGFVGYDMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYMVFDHKKEKIHVIEDALYSERSQEALEKSLNQV
                     LEELRIPAPNEFEDLDLSPLDFKPHIAPHKFEGMVETARDLIRNGDMFQCVLSQRFSA
                     EVTGNPFDFYRNLRVTNPSNYLYFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGATDEEDKTLATDLLSDEKETAEHRMLVDLGRNDIGRISETASVQVTKYMKVELFRY
                     VMHLTSVVKGRLLPELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRGVYAGAI
                     GYLSATGDMDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGE
                     LRP"
     gene            1801..2367
                     /locus_tag="ACL73L_001675"
     CDS             1801..2367
                     /locus_tag="ACL73L_001675"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HGS0772616.1"
                     /translation="MILLIDNYDSFTYNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMIRDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEEGRAVMRYHSILIEEMPEDFEVTARSTDDQAIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEKVVK"
     gene            2378..3382
                     /gene="trpD"
                     /locus_tag="ACL73L_001676"
     CDS             2378..3382
                     /gene="trpD"
                     /locus_tag="ACL73L_001676"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HGS0772617.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIVTGRVTEAQIASLLLALK
                     MKGETPEERTAIAQVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNMGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLSSFTPEDLGMEGYAMEDIRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVALARQVIARGKALEKLRLLQEYQK
                     "
     gene            3379..4146
                     /gene="trpC"
                     /locus_tag="ACL73L_001677"
     CDS             3379..4146
                     /gene="trpC"
                     /locus_tag="ACL73L_001677"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HGS0772618.1"
                     /translation="MSQEFLARILEQKVREVEQMKLEQIQPLRQTYRLAEFLKNHQDR
                     LQVIAEVKKASPSLGDINHDVDIVQQAQTYEENGAVMISVLTDEVFFKGHLDYLREIS
                     SQVEIPTLNKDFIIDEKQIIRARNAGATVILLIVAALSEERLKELYDYATELGLEVLV
                     ETHNLAELEVAHRLGAEIIGVNNRNLTTFEVDLQTSVDLAPYFEEGRYYISESAIFTG
                     QDAERLAPYFNGILVGTALMQAENVAQRIKELQIDKG"
     gene            4133..4732
                     /locus_tag="ACL73L_001678"
     CDS             4133..4732
                     /locus_tag="ACL73L_001678"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HGS0772619.1"
                     /translation="MTKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEAAE
                     LAKLIPADVKKVGVFVSPSRAELLEAIDKVGLDLVQVHGQVADDLFENLPCASIQAVQ
                     VDGEGHVPNSQADYLLFDAPVAGSGQPFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQ
                     HFTPYAVDVSSGVETDGQKDHEKIRRFIERVKNGISRTK"
     gene            4710..5933
                     /gene="trpB"
                     /locus_tag="ACL73L_001679"
     CDS             4710..5933
                     /gene="trpB"
                     /locus_tag="ACL73L_001679"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HGS0772620.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLRQYVGRETPLYYAKNLTQHIDGAKIYLKREDLNHTGAHKINNALGQVWLA
                     KRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
                     EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQ
                     QYRDLTGRDLPDALVACVGGGSNAIGLFHPFVEDESVAMYGTEAAGLGVDTEHHAATL
                     TKGSPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTD
                     EEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVK
                     DRLEADAAKKGEAHA"
     gene            5926..6702
                     /gene="trpA"
                     /locus_tag="ACL73L_001680"
     CDS             5926..6702
                     /gene="trpA"
                     /locus_tag="ACL73L_001680"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HGS0772621.1"
                     /translation="MPKTLTEKLNAIKATGKGIFVPYIMAGDHEKGLDGLAETIHFLE
                     DLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIETEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLADTDIALIPLVSLTTGI
                     ERQKELIEGAEGFIYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQA
                     DLERFNAVSDGVIVGSKIVKALHQGEPIQDFIRQAVAYQK"
     gene            complement(6674..6775)
                     /locus_tag="ACL73L_001681"
                     /pseudo
     CDS             complement(6674..6775)
                     /locus_tag="ACL73L_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            7004..7735
                     /locus_tag="ACL73L_001682"
     CDS             7004..7735
                     /locus_tag="ACL73L_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772622.1"
                     /translation="MLNQDLFDSLEAQKIVDTLMKGQKDYVDERLEKRETMIVSNGYA
                     WTRPNHIDTAFASADLFEYKLQLAGQTWGYLEFETNTEKYGKVLLIIKGKKRLTNQFP
                     LVQKNKSGYLFEYAQMNTLYLNQHSSYKNDENSHSFPIQMELVSDEMIQEIEQATKNS
                     NIEKFMILTYEADSENNIISVDVVMPDARTGQLHLIQDLSEYIQSSSYHFEEAKYQDI
                     PNFSELSETEDFEIIPRIEKQEGQK"
     gene            7750..8901
                     /locus_tag="ACL73L_001683"
     CDS             7750..8901
                     /locus_tag="ACL73L_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGS0772623.1"
                     /translation="MFNGRVLKELRLLNGLSRAELAQRINLTEQAIWQFESNETKPKL
                     STKMHLANQFHVDLTYFEQEEESIRFDSSVIAFRNADLATRKTIDIQTMYLHKVDSLI
                     DYFESFVIIPNIIIHDLSNVVSESYHKGESIEELALYAREKLGISKDNHDLLYKLERS
                     GIYIVERLINGQADAYSAWSKLGRPYIVLGTNKSSVRRNFDLAHELGHILLHKYKDMN
                     EDGDRLEQEANYFASCFLLPKKEFLVKFEERVGKRVSNPDSYILLKSDLNVSIQALEY
                     RAFKLGLLTPKQHSYFYRQIAQKGYKMIEPLDDQIFVKKPSKVKSILDVVLSNHLVSL
                     ATIMSKQSIRLQFISEIFSVEMKFFDQYQEDRRTDRFDNIIPLYKRNNL"
     gene            complement(8953..9612)
                     /locus_tag="ACL73L_001684"
     CDS             complement(8953..9612)
                     /locus_tag="ACL73L_001684"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HGS0772624.1"
                     /translation="MIDFYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTPLR
                     PLDLIPILSQVFNRFRCRYCKVRYPVWYALFELGLGLLFLLYSWELLSLGQVVLITAG
                     LTLSIYDFRHQEYPLLVWMTFHLILIASSGWNLVMVSFLALGILAHFIDIRMGAGDFL
                     FLASCALVFSITELLILIQFASATGILAFLLQKKKERLPFVPFLLLATCVIIFGKLLL
                     V"
     gene            9682..>10103
                     /locus_tag="ACL73L_001685"
     CDS             9682..>10103
                     /locus_tag="ACL73L_001685"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS0772625.1"
                     /translation="MTIRFEEKVSIENAQFVCQWSNSLGKSFQEQWMGSRIPFLLTLQ
                     ALEEVFSIFDEQEFVGLIQKIRLEDSNLHIGRFFINPQKQGQGLGSQALRKFVSLAFE
                     NEDIDSISLNVFEANQRAQNLYQKEGFEIVQMVEAPVR"
BASE COUNT         3155 a         1842 c         2379 g         2727 t
ORIGIN      
        1 atacctacga taaggcgacg ttgacgcggt ttgaagagat tttcgaagag tataaaggtg
       61 taccgagatg aacatctctt catcaatgtc tctggtcgtg gaggcaagga cctagctgcg
      121 attgcagatt actagaagct aaaaaataat agatggaaaa attacagttc tttctctcac
      181 agagagcttg tggttgctga aaacaagcag agaaagctgt aaagttgggt tcatctgaaa
      241 cgagagaaga aaacatcatt ctcaagggag tgccctttat cgcacagcct gttacaggag
      301 atatctgaga caggaatgag agataggaga aatcctataa ttgaggtggc accgcgaatt
      361 tcgtcctcac gcaagttatt ttgcgtgggg atttttcata cagtcaccac ggactagaag
      421 tagaactgaa agggaaatta caatggaacg aatcattcat ggagatgtct tatcaccaat
      481 cttggcttat atgcgcttaa aggggcaaca taaggttatt ttagagagta tttcgagaga
      541 caaggaaaca gctcgttttt ctatcctagc ctataatcca gtttttgaga ttaagtttga
      601 aaatggggtt ctctatcaaa atggtcaagt gattgatcgg gatcccttgg atttccttta
      661 tgaagtgatt cataagagtc agcaccactc agagctacct tttggtggtg gagccattgg
      721 ttttgtgggt tacgatatga tttcgcttta tgaagaaatt ggtcaaatcc ctgaggatac
      781 aattgggacg ccagacatgc atttctttgt ctatgagagc tatatggtct ttgaccacaa
      841 gaaggagaag atacatgtca tagaggatgc actttatagt gagcgcagtc aagaggcctt
      901 ggaaaaatcc ttgaaccaag tgcttgagga attacgcatt cctgctccaa atgaatttga
      961 agatttggat ctatctccgt tagacttcaa accgcatatc gctccacata agtttgaggg
     1021 aatggtagaa acagctcgtg acttgattcg taacggagat atgttccaat gtgtactcag
     1081 ccagcgtttt tcagcagaag ttactggaaa tccatttgac ttctacagaa atctccgcgt
     1141 gactaatcct tctaattacc tttatttcta tgattttggg gattatcaaa tcatcggagc
     1201 tagtccagaa agtttagttt ctgtcaaaaa tggcatcgtg acaaccaatc cgattgcagg
     1261 gacgcgacca agaggggcta cggatgaaga agacaagacc ttggcgactg acctcctctc
     1321 tgatgagaag gaaacagcag aacatcgaat gttggtagac ttggggcgta acgatattgg
     1381 ccgcatctct gaaacggcca gtgtccaagt tactaagtat atgaaggtgg agctcttccg
     1441 ctacgtcatg catttgacca gcgtggtcaa ggggcgtttg cttccagaac tcacggccat
     1501 ggatgccttg aaagctacac ttcctgctgg aaccgtttct ggagcaccaa agattcgggc
     1561 gatgagacgc atctatgaac tggaaacgga aaaacggggc gtatatgcag gagcaatcgg
     1621 ctacttgtct gcgacgggtg atatggattt ggccattgcc atccgaacca tgattctcaa
     1681 aaatcaaaga gcctatgtgc aggctggggc agggattgtc tacgactcta tcgcccaaaa
     1741 cgaataccaa gaaaccatta acaaagcaaa atctatgact agaattggag aactaagacc
     1801 atgattttat tgattgacaa ctatgattct tttacctata acttggccca atacattggg
     1861 aattttgcag aagttcaggt tctgagaaat gatgattcca agctgtatga agaagctgaa
     1921 aaagcagatg gtctggtctt ttctcctggt cctggttggc cagttgatgc tggaaagatg
     1981 gaagacatga ttcgtgattt tgcaggcaag aagccgattc ttgggatttg tttgggccac
     2041 caagccattg cagaagtctt tggtggtaag ctaggtttgg ctccaaaagt catgcatggg
     2101 aaacagagca atatcaactt tgaagcgcca tctgttttgt atcaaggtat tgaggaagga
     2161 cgtgcggtca tgcgttatca cagtattttg attgaggaaa tgccagaaga ctttgaagtg
     2221 acagctcgtt cgactgatga ccaagccatc atggggattc aacataaaaa cctaccgatt
     2281 tatggcttcc agtaccatcc agagagcatt ggaacgccag acggcttgtc ttctattcgg
     2341 aattttatcg agaaggttgt aaagtgagga aactaggatg aaagagatta ttgaaaaact
     2401 agcaaaattt gaaaatttat caggtgtgga aatgacggat gtcattgagc gtatcgtaac
     2461 tgggcgtgta actgaagcgc agattgcttc tctcctctta gctcttaaga tgaaggggga
     2521 aacacctgaa gaacgcacag ccattgccca agtcatgaga ggacatgccc agcatattcc
     2581 aactgagatt catgatgcca tggacaactg tggtacaggt ggggacaagt ctttcagttt
     2641 taatatttcc acaactgcag cctttgtctt ggctggtggc ggtattcaca tggccaagca
     2701 tggtaaccgc tcgatttctt ctaaatctgg ttccgcagat gtcctcgaag ccttgggaat
     2761 caatcttgac ctcaaaccag ctgaactagg taaggtcttt gataaaactg gaatcgtctt
     2821 tctcttcgct aaaaatatgc acccagctat gaaatacatc atgccagctc gtttggaatt
     2881 gggaattcca acgattatga acttgactgg tccactgatt catccaatgg ctttagaaac
     2941 acagcttctt ggaattagtc gtccagaact cctagaaagt acagctcagg ttttgaaaaa
     3001 tatgggtcgc aaacgtgcca tcgtggttgc tggaccagaa gggttggatg aagctggctt
     3061 gaacggaaca accaagattg cacttcttga aaatggcgaa atcagcttgt caagctttac
     3121 tccagaggat ttgggaatgg aaggctatgc tatggaagat attcgtggtg ggaatgctca
     3181 ggaaaatgca gaaattttgc ttagcgttct gaaaaacgaa gcaagtccat tcttggaaac
     3241 gacagtcttg aatgctggtc ttggtttcta tgctaatggt aagattgata gcatcaagga
     3301 aggagttgcc ttggcccgtc aagtgattgc tagaggcaag gcccttgaaa aactcagact
     3361 gttacaggag taccaaaaat gagtcaggaa tttttagcac gaatcttaga gcagaaggtg
     3421 cgtgaggtgg agcagatgaa gctggagcaa atccagcctc tgcgccagac ctatcgcttg
     3481 gcagagtttt tgaagaatca tcaggaccgc ttgcaggtaa tcgctgaggt caagaaagct
     3541 agccctagtt tgggagatat caatcacgat gtggatattg tgcaacaggc ccagacttat
     3601 gaagaaaacg gagcagtgat gatttcggtg ttgacagatg aggttttctt taaagggcat
     3661 ttggattatc tacgggaaat ttccagtcag gtagagattc cgacgctcaa caaagacttt
     3721 atcatcgatg aaaagcaaat catccgcgct cgcaatgcag gtgcgacagt tatcttgctc
     3781 attgtggcag ccttgtccga agaacgcctc aaggaactgt atgactacgc gacagagctt
     3841 ggtctggaag tcttagtgga gactcacaat ctagctgaac tagaggtagc ccacagactt
     3901 ggtgctgaga ttatcggggt caacaaccgc aacttgacta cctttgaggt cgacttgcag
     3961 accagtgtag atttagcccc ttactttgag gaaggtcgct attacatttc tgaatctgcc
     4021 attttcacag ggcaggatgc ggaacgacta gccccatact ttaacggaat tttggtaggg
     4081 acagctctta tgcaggcaga aaatgtggcc cagagaatca aggagttgca gattgacaaa
     4141 ggttaaaatt tgtggactat cgaccaaaga agcggtggaa acagccgttt cagcaggagc
     4201 cgactatatc ggttttgtct ttgcacctag taaaagacag gtgactttag aagaggcagc
     4261 tgagttggca aagcttattc ctgcagatgt caaaaaggtt ggtgtatttg tttcaccaag
     4321 tcgggcagaa ctgctggaag cgattgacaa agttggcttg gacttggttc aagttcacgg
     4381 tcaggtggca gatgatttat ttgagaattt gccttgtgct agcattcagg ctgtgcaggt
     4441 agatggagag ggtcatgtgc ccaattctca ggcagactat ctactctttg atgcccctgt
     4501 ggctgggagt ggccagccct ttgattgggg tcaactggat acgactggac tagcacagcc
     4561 cttctttatc gcaggtggcc ttaatgaaga taatgtagta aaagcaattc aacactttac
     4621 tccctatgca gtagatgtat caagcggagt ggagacagat ggacaaaaag atcatgaaaa
     4681 gattagaaga tttatagaga gggtaaagaa tggcatatca agaaccaaat aaagatggat
     4741 tttacggaaa atttggcgga cgttttgtcc cagaaacatt gatgacagca gttttggagt
     4801 tggagaaggc ctaccgtgaa agtcaggcag acccaagttt ccaagaggaa ttaaaccagc
     4861 ttttgcgcca gtatgtggga cgtgaaactc ccctttacta cgcaaaaaac ttgacccagc
     4921 atatcgacgg agccaagatt tatctcaaac gggaagacct taaccataca ggagcccaca
     4981 agattaacaa tgccttagga caagtttggc ttgccaaacg catgggtaaa aagaaaatta
     5041 tcgcagaaac tggtgctggt cagcacggtg tggcaactgc aactgctgcg gccctcttta
     5101 acatggaatg taccatctac atgggtgagg aagatgtcaa acgccaagcc ctcaatgtct
     5161 tccgtatgga gcttttggga gctaaggtcg aggccgtgac agatggttcg cgcgtgctca
     5221 aggatgcggt caatgcagcc cttcgttcat gggtggctaa tatcgacgat acccactata
     5281 tccttggttc tgccttgggg cctcatccat ttccagaaat cgttcgtgac ttccaaagtg
     5341 tcatcggtcg agaggctaaa caacagtacc gtgacttgac aggtcgagat ctgccagatg
     5401 ccctagtagc ctgtgttggt ggtgggtcga atgctatcgg gctcttccat ccctttgtag
     5461 aagatgagtc agtagctatg tatggaactg aagcagcagg gcttggtgtg gatacagagc
     5521 accacgctgc taccttgacc aagggtagtc cgggtgttct tcacggttct ctcatggatg
     5581 tgctccaaga tgcccatggt caaattcttg aagccttctc tatctcagca ggtttggact
     5641 atcctggtat cggtccagag cattctcact accacgatat caaacgtgcc agctatgtcc
     5701 ctgtgacaga cgaagaagct ttggaaggat ttcaactctt gtctcgtgtg gaagggatta
     5761 tcccagcctt ggaatctagc catgctatcg cttttgcggt gaaattggcc aaagaacttg
     5821 gaccagaaaa gtctatgatt gtatgcctat caggtcgtgg ggacaaggat gtggttcaag
     5881 tcaaagaccg cttggaagca gatgcagcaa agaagggaga agctcatgcc taagacacta
     5941 acagaaaaat tgaatgctat aaaagcaact ggaaaaggaa tttttgttcc ctatatcatg
     6001 gctggtgacc acgagaaagg tttggacggt cttgctgaaa caattcactt tttagaagat
     6061 ttgggtgtct ctgcaattga agtgggtatt cccttttcag accctgttgc agatggacct
     6121 gttatcgaag aagctggctt gcgcagttta gcccacggga cctctaccca ggctttagtt
     6181 gaaaccttga aaaccattga aacagagatt ccactggtca tcatgaccta cttcaacccc
     6241 ctctttcagt acggtgtgga gaactttgtc aaagatctgg ctgatacagc tgtgaagggc
     6301 ttgattatcc cagacctgcc tcatgaacat gccaactttg tagaaccatt tttggcagat
     6361 acagatatcg ccttgattcc tttagtaagc ttgaccacag gaattgagcg tcagaaagag
     6421 ttgattgaag gggcggaggg attcatctat gccgttgcca tcaatggagt gacagggaaa
     6481 tcaggcaatt accgtgcaga tttggacaag cacttggcgc aacttcatca agtggccgac
     6541 atcccagtct tgacaggttt tggtgtatct agtcaagccg atctggaacg cttcaatgcg
     6601 gtgtcagatg gtgttatcgt cggttcaaaa atagtaaaag ctctccacca aggagagccg
     6661 attcaggatt ttatcagaca agcagtagct taccaaaaat aatcaaacaa gtagcaagta
     6721 agaggaaagg cacgaaagga agtctttcct ttttcttttg caggagaaag gtcagtttta
     6781 tagtgaattg aatctggaat attacactac ggttcctaaa atattttcag aaagcgattt
     6841 gaatttccca ataaatttgt tcagttttta attgatttta ctatagaaga tataattttc
     6901 taaattactc tgacacagaa ctttaagaaa aagataatta ttgtagtaat ttctttaaga
     6961 aaatggtata attcattaaa aagtatttgg aggaggaaga attatgttga atcaggatct
     7021 ctttgattcg cttgaggcac aaaaaattgt agatactttg atgaaagggc aaaaagatta
     7081 tgtagatgaa cgtctagaaa aaagagagac aatgatagtg tcgaatggtt atgcatggac
     7141 gcgacctaat catattgata ctgcatttgc atcagcagat ttgtttgagt ataaattaca
     7201 attagcagga cagacttggg gatatttaga atttgaaaca aatacagaaa aatatgggaa
     7261 agtattgtta attataaagg gtaagaagcg acttacgaac caatttcctt tggtacaaaa
     7321 aaataagagt ggctacttat ttgaatatgc tcagatgaat acactttatc ttaatcaaca
     7381 ttcttcctac aaaaatgatg aaaatagtca ttcctttcca attcagatgg agttagtttc
     7441 tgatgaaatg attcaagaaa ttgaacaagc tactaaaaat tcgaatatcg aaaaatttat
     7501 gattttaact tatgaggcgg actcagaaaa caatattata tctgtagatg ttgttatgcc
     7561 tgatgcacga actggtcagt tacacttgat tcaagatttg tctgagtata ttcaatcaag
     7621 ttcgtaccat ttcgaagaag ctaaatatca agatattcct aatttttcag aattatcgga
     7681 aacagaagat tttgaaatta ttccaagaat agaaaaacaa gaaggtcaaa agtagtaagg
     7741 agtatgtaaa tgtttaatgg tcgggtattg aaagaattac ggctgttaaa tggtttaagt
     7801 agagcagaat tagctcagag aattaattta acggaacaag ccatttggca gtttgagtcc
     7861 aacgaaacga aacctaaatt atcaaccaaa atgcatttgg ccaaccaatt tcatgttgat
     7921 ttaacttatt ttgaacagga agaagagagc attcgatttg attcttctgt aattgccttt
     7981 agaaatgcag acctagcaac acggaaaaca atagatattc aaactatgta tttacataag
     8041 gtagatagtt tgattgatta ttttgaaagt tttgtaatta tacctaatat tataattcat
     8101 gacctaagta atgtagtgag tgaatcttat cataagggag aatccattga ggaattggct
     8161 ctttatgcca gggaaaaatt aggtatttca aaagataatc atgatttgct ttataaatta
     8221 gaacgttcag gcatctatat cgtggaacga ttaattaatg gccaagctga tgcttatagc
     8281 gcatggtcaa aattgggaag accttatatt gtgttaggaa cgaataaatc atctgtacgt
     8341 cgaaattttg acttagctca tgagctagga catattcttt tacataaata taaagatatg
     8401 aatgaagatg gcgatcgttt ggagcaagaa gcaaattatt ttgcatcatg ttttttattg
     8461 ccaaaaaaag agtttttagt caaatttgaa gagagggttg gcaagcgtgt cagcaatcct
     8521 gatagttata ttttattgaa gtcggatttg aatgtttcga tacaggcttt agagtatcga
     8581 gcttttaagt taggattatt gactccaaag caacattctt acttttatcg tcaaattgcg
     8641 caaaaaggtt acaaaatgat tgaacctttg gatgatcaaa tttttgttaa aaaaccaagc
     8701 aaagtaaaga gtattctgga cgtcgttttg agtaatcatc tagtcagtct agcgactata
     8761 atgtctaaac aaagtattcg tttacagttt ataagcgaaa tattttcagt cgaaatgaaa
     8821 ttttttgatc agtatcaaga agatagaaga acagatcgat ttgataacat catccctttg
     8881 tacaaaagaa ataatttata atttaactgt gaaataattt catgaaggac atatggcaga
     8941 aattggattt tatcagacaa gcagtagctt accaaaaata atcacacaag tagcaagtaa
     9001 gaggaaaggt acgaaaggaa gtctttcctt tttcttttgc aggagaaagg ctaggatacc
     9061 cgtcgcagaa gcaaactgaa tcaagatcag taactccgtt atgctaaaga cgagagcaca
     9121 agaagctaaa aagaggaaat cccctgcgcc catgcggata tcgataaaat gagccaaaat
     9181 tccaagagca aggaaggaga ccatgaccag attccagcca gaggaagcta ttaggattag
     9241 gtggaaagtc atccagacca gtaagggata ttcctgatgg cgaaagtcat agatgctcaa
     9301 ggtcaaacca gcggtgatta ggacgacttg ccccaaggaa agcaattccc aagagtaaag
     9361 cagaaagagg agtcctaagc ctagttcaaa gagggcatac cagacaggat agcggacctt
     9421 gcagtagcga cagcgaaagc gattgaagac ctgtgagaga atcggaatca aatctaaggg
     9481 acgcaaggga gtctgacagg aatcgcagtg actggctgaa ctgataatgg attgctctgg
     9541 aaaacggtca atgaccaaac caagaaagga agcgagaatg ctcccgacga gaaaaaaata
     9601 aaaatcaatc atacttatct attcgtaaaa aatgggagaa atagtataat ggagaaacat
     9661 agataagatg gtgaggaaaa gatgacaatt cgttttgaag aaaaggtgag tatagaaaac
     9721 gctcaatttg tatgccaatg gtccaactcc ctaggcaaat cctttcaaga acaatggatg
     9781 ggatcaagga ttccttttct actgaccctt caagccttgg aagaagtctt ttcaatcttt
     9841 gatgaacaag agtttgtggg acttatccag aaaatcaggc tagaagacag caatcttcat
     9901 atcgggagat tttttatcaa cccccagaaa caggggcaag gcttaggtag ccaggcttta
     9961 aggaaatttg ttagtttggc ctttgaaaat gaagatatag atagtatttc tctaaatgtc
    10021 ttcgaggcaa atcaaagagc tcagaatctt taccaaaaag aaggatttga aatcgttcaa
    10081 atggttgaag cacctgtacg aaa
//
LOCUS       DBERDK010000068         9940 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.068, whole genome shotgun sequence.
ACCESSION   DBERDK010000068 DBERDK010000000
VERSION     DBERDK010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 9940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9940
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.068"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            480..1523
                     /locus_tag="ACL73L_001686"
     CDS             480..1523
                     /locus_tag="ACL73L_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HGS0772626.1"
                     /translation="MTNNKLYSLIEFRNKIYEELELSRSDLAILLCAMLIASIGLNMD
                     STPVIIGAMLISPLMTPILGVGLSLAIFDFKLLRKSFKILAIQILASLIASTLYFYLS
                     PISYASSEIVARTSPTIWDVLIAFVGGIAGIIGARKKETNNIVPGVAIATALMPPLCT
                     VGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRLMMVKKHYFKDNEEDSKMRRIL
                     LLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQGYNILKKTYSKKTHTLKLTIS
                     GNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQLSKEELVEYFFQYIKDKEAKEK
                     EKANKFDTESEEQ"
     gene            1690..3777
                     /gene="topA"
                     /locus_tag="ACL73L_001687"
     CDS             1690..3777
                     /gene="topA"
                     /locus_tag="ACL73L_001687"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HGS0772627.1"
                     /translation="MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKMKLTSNDEVKEVLSRLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQNEAASFITDRFGS
                     KYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQNGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMVVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEEKIK"
     gene            3998..4357
                     /locus_tag="ACL73L_001688"
     CDS             3998..4357
                     /locus_tag="ACL73L_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HGS0772628.1"
                     /translation="MRLIYLIIGFLSLALAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKLYQAYVADFRETKSIARERKKKIIVSIYVLMGISIYFAPLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
     gene            4450..5082
                     /locus_tag="ACL73L_001689"
     CDS             4450..5082
                     /locus_tag="ACL73L_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HGS0772629.1"
                     /translation="MIYEFCAENVTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPRGGDFVYNDLEIAIMLEDIRLTAQAGSQGVVFGALTADKK
                     LDKPNLEKLIAASKGMEIVFHMAFDELSDEDQPEAIDWLSQAGVTRILTRAGVSGDSL
                     EKRFVHYHRILKYAKGKIEILPGGGIDLENRQTFIDQVGVTQLHGTKVVF"
     gene            5126..5782
                     /locus_tag="ACL73L_001690"
     CDS             5126..5782
                     /locus_tag="ACL73L_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HGS0772630.1"
                     /translation="MFEIFKSYQFNQEKAHDYGFIENSEVWTYSCQILQGDFVMTVSI
                     TADNVNFQVFDQETGDLYPHVYMESMRGSFVGNVREACLEILYQIRKACFDVQDFICH
                     QTKRIMTQVQEKYGNQLEYLWEKSPDTAVLRHEGNQKWYAVLMKISWNKLEKGREGQV
                     EAVNLKHDQVANLLSQKGIYPAFHMSKRYWISVSLDDTLSDEEVLELIEKSWNLTSKK
                     "
     gene            5852..6895
                     /locus_tag="ACL73L_001691"
                     /pseudo
     CDS             5852..6895
                     /locus_tag="ACL73L_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261078.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
     gene            6907..7944
                     /gene="leuB"
                     /locus_tag="ACL73L_001692"
     CDS             6907..7944
                     /gene="leuB"
                     /locus_tag="ACL73L_001692"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HGS0772631.1"
                     /translation="MAKKIVALAGDGIGPEIMEAGLEVLEALAEKTGFDYEIDRRPFG
                     GADIDAAGPPLPDETLKASREADAILLAAIGSPQYDGAVVRPEQGLMALRKELNLYAN
                     IRPVKIFDSLKHLSPLKLERIAGVDFVVVRELTGGIYFGDYILEERNARDINDYSYEE
                     VERIIRKAFEIARNRRKIVTSIDKQNVLATSKLWRKVAEEVAQDFPDVTLEHQLVDSA
                     AMLMITNPAKFDVIVTENLFGDILSDESSVLSGTLEVMPSASHSENGPSLYEPIHGSA
                     PDIAGQGIANPTSMILSVAMMLRDSFGRYEDAERIKRAVETSLAAGILTRDIGGQAST
                     KEMTEAIIARL"
     gene            7941..8210
                     /locus_tag="ACL73L_001693"
     CDS             7941..8210
                     /locus_tag="ACL73L_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HGS0772632.1"
                     /translation="MKLDEKIALVLLIWNVIIFLIYGIDKFKARRRAWRIQEKILLIL
                     ALICGGFGAWLAGITFHHKTRKWYFKTVWFLGMVTTLVALYFIWR"
     gene            8214..8573
                     /locus_tag="ACL73L_001694"
     CDS             8214..8573
                     /locus_tag="ACL73L_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HGS0772633.1"
                     /translation="MAGSSREHAAWVLADYGFKVVIAGSFGDIHYNNELNNGLLPIVQ
                     PREVREKLAQLKPIDQATVDLEQQKIISPVEEFTFEIDSEWKHKLLNTLDDIGITLQY
                     EELIAAYEKQRPAYWQD"
     gene            8735..9305
                     /locus_tag="ACL73L_001695"
                     /pseudo
     CDS             8735..9305
                     /locus_tag="ACL73L_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004915491.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
     gene            9399..9464
                     /locus_tag="ACL73L_001696"
                     /pseudo
     CDS             9399..9464
                     /locus_tag="ACL73L_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849983.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor (SUA5)"
     gene            9627..>9940
                     /locus_tag="ACL73L_001697"
     CDS             9627..>9940
                     /locus_tag="ACL73L_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772634.1"
                     /translation="MNKRKVSLEDFYKWYSLNKEELLNKATVGEKFNDKLKEEFLQEW
                     PLDRILTMSIDEYVIGKGQQNKSLCYALEKGKYKNLFLGISGGSASKFGIYWNKKTNK
                     YK"
BASE COUNT         3122 a         1708 c         2202 g         2908 t
ORIGIN      
        1 actgttatag attcaataca caataattta gtgtaattga gaaaggagaa atgattgtga
       61 ttgatgttgg ctaggttatg ttcaatgatt cctaccgtct caaatcttgt cagtaaggaa
      121 aaataaattc ttcaaaagta gagattacaa ggcttgttta agaaagaatt caaagacctt
      181 gacaaataaa aataaaatgg ttattataaa aaatggtctg aaatagatga tgatactttt
      241 cgaaaatctt tttacgtcag ctcagctttg ccttgctgtg ttttgagcaa gctacggtta
      301 gcttccgagt ttgattttca tttactagaa atgaaactga tgagagatat cagtagacat
      361 ctgagttagg atattatgga aaatgataaa aagagctcgt gagattggca tatcagacta
      421 ctaaagtatt gagtttgtta ggattttagc gactagttag ctgggaaagg aagatatttg
      481 tgacaaataa taaactgtat tcgttgatag aatttagaaa taaaatatat gaagaattag
      541 aactttccag aagtgattta gcgattttac tatgtgccat gcttatcgcc tctatcggat
      601 taaatatgga ttcgactccc gtgattattg gagccatgtt aatctctcct ttgatgacac
      661 ctattctggg agtggggctc tctctagcta tatttgattt taaattgtta agaaaatctt
      721 ttaaaatatt agctattcaa attcttgcca gtctaatagc ttcaacactt tatttttatc
      781 tttctcccat ttcgtatgct agttcggaga ttgttgctag aacctctccg actatttggg
      841 atgttctcat tgcttttgta ggagggatag caggtattat tggtgctagg aaaaaagaga
      901 ccaataatat tgttcctggt gttgctattg caaccgcctt gatgcctcct ctttgtacag
      961 taggttatgc tattgcttct gctaatctaa aatttatcat aggctcctct tacctattcc
     1021 tcatcaattg tagctttatt gtcattgcga cttatatagg tgttaggttg atgatggtta
     1081 agaaacatta ttttaaagat aatgaagaag actctaaaat gcgtaggatt ttgcttctag
     1141 ttgctgtttt gctgatgatt ccgagtttca tctctgcaac gactttagtg agagaaacgt
     1201 tgaaaaaaga gtcccttaag aaatttatat cagagcagtt tcaggggtat aatattttga
     1261 aaaaaaccta ttctaaaaag actcataccc taaagctaac catttcagga aattatttga
     1321 cagaagaaga actcgatatg atttcaagta agagaggtga ctatggttta agtgatgttt
     1381 ctgttcaagt ttcacaattg tctgattcag aacaacttag taaggaagag ctggtggagt
     1441 atttcttcca gtatatcaag gataaggaag caaaagaaaa ggagaaagct aataaatttg
     1501 atacagagtc tgaggagcaa taatttcttg agaatagctg gtttctctcg tgagtcttct
     1561 atgtatatca aaggaagact gaggttttaa gtataaaact tttcttctat tatagtagct
     1621 aagtcttgac agggttcaaa aaatggttta cactttataa agtttattac tttgaaaagg
     1681 tgtgatactg tggctacggc aacaaaaaag aaaaaatcaa cagttaaaaa aaatctagtc
     1741 atcgtggagt cgcctgctaa ggccaagacg attgaaaaat atctaggcag aaactacaag
     1801 gttttagcca gtgtcgggca tatccgtgat ttgaagaaat ccagtatgtc cgtcgatatt
     1861 gaaaataatt atgaaccgca atatatcaat atccgaggaa aaggccctct tatcaatgac
     1921 ttgaaaaaag aagctaaaaa agctaataaa gtttttctcg cgagtgaccc ggaccgtgaa
     1981 ggagaagcga tttcttggca tttggcccat attctcaact tggatgaaaa tgatgccaac
     2041 cgtgtggtct tcaatgaaat caccaaggat gcagtcaaaa atgcttttaa agaacctcgt
     2101 aagatcgata tggacttggt cgatgcccaa caagctcgtc ggatcttgga tcgcttggta
     2161 gggtattcga tttcgcctat tttgtggaag aaggtcaaga agggcttgtc agcaggtcgc
     2221 gttcagtcca ttgcccttaa actcatcatt gaccgtgaaa atgaaatcaa tgccttccag
     2281 ccagaagaat actggacagt tgatgctgtc tttaaaaagg gaaccaaaca atttcatgct
     2341 tccttctatg gagtagatgg taaaaagatg aaactgacca gcaatgacga agtcaaggaa
     2401 gtcttgtctc gtctgacgag taaagacttt tcagtagatc aggtggataa gaaagagcgc
     2461 aagcgcaatg ctcctttacc ctataccact tcatctatgc agatggatgc tgccaataaa
     2521 atcaatttcc gtactcgaaa aaccatgatg gttgcccaac agctctatga aggaattaat
     2581 atcggttctg gtgttcaagg tttgattacc tatatgcgta ccgattcgac tcgtatcagt
     2641 cctgtagcgc aaaatgaggc ggcaagcttc attacggatc gttttggtag taagtattct
     2701 aagcacggta gcaaggtcaa aaatgcatca ggtgctcagg atgcccatga ggctattcgt
     2761 ccgtcaagtg tctttaatac accagaaagc atcgctaagt atctggacaa ggatcagctc
     2821 aagctatata cccttatctg gaatcgtttt gtggctagcc agatgacagc ggccgttttt
     2881 gataccatgg ctgttaaatt gtctcaaaat ggagttcaat ttgctgccaa tggtagtcag
     2941 gttaagtttg atggttatct tgccatttat aatgattctg acaagaataa gatgttaccg
     3001 gacatggttg ttggagatgt ggtcaaacag gtcaatagca aaccagagca acatttcacc
     3061 caaccgcctg cccgttattc tgaagcaaca ctgattaaaa ccttagagga aaatggggtt
     3121 ggacgtccat caacctatgc gccaaccatt gaaaccattc agaaacgtta ttatgttcgc
     3181 ctggcagcca aacgttttga accgacagag ttgggagaaa ttgtcaataa gctcatcgtt
     3241 gaatatttcc cagatatcgt aaacgtgacc ttcacagctg aaatggaagg taaactggat
     3301 gatgtcgaag ttggaaaaga gcagtggcga cgggtcattg atgcctttta caaaccattc
     3361 tctaaagaag ttgccaaggc tgaagaagaa atggaaaaaa tccagattaa ggatgaacca
     3421 gctggatttg actgtgaagt gtgtggcagt ccaatggtca ttaaacttgg tcgttttggt
     3481 aaattctacg cttgtagcaa tttcccagat tgccgtcata cccaagcaat cgtgaaagag
     3541 attggtgttg agtgtccaag ctgtcatcag ggacaaatta ttgagcgaaa aaccaagcgt
     3601 aatcgcctct tctatggttg caatcgctat ccagaatgtg aatttacctc ttgggacaag
     3661 cctgttggtc gtgactgtcc aaaatgtggc aacttcctca tggagaaaaa agtccgtggt
     3721 ggtggcaagc aggttgtttg tagcaaaggc gactacgagg aagaaaagat taaataagag
     3781 gaggggcccg aaagtaaatt tcaggctcct tttggttaga acttgacaaa atacatcatt
     3841 ttatgcgaaa ctagaagaag attattttat actcaatgag aatcaaagag caaattagaa
     3901 agctagtcgc aggttgctca aagcactact ttgaggttga agatggaagc tgacctggtt
     3961 tgaagagatt ttcgaagagt attaactagg agaagttatg cgtcttatct atctgataat
     4021 tggtttttta tcactggcct tggctattgt tggggttgtt ttacccttgt tgccgacaac
     4081 acctttcctt ttgttgtcta ttgcttgttt ctccagaagt tccaagcgat tcgaagattg
     4141 gctttatcat accaagctct atcaagcata tgtagctgat tttcgtgaga ccaagtctat
     4201 tgcgcgtgaa cgaaagaaaa aaatcatcgt ctctatctac gtcttgatgg gaatttctat
     4261 ttattttgca cctcttttac cagtcaaaat cggtctgggt gctttgacca tctttattac
     4321 ttattatctc ttcaaggtca ttccagacaa agaatagtta aaacagtagt tatttgcctt
     4381 gataaaattg aaagcatatt cataacaata tgatataata aaattgaagt aatattcaag
     4441 gagaatcaaa tgatttacga attttgtgct gaaaatgtga ctttacttga aaaagcgatg
     4501 caggctggag ctcgtcggat tgaactctgt gataatctag cagttggtgg gacaacaccc
     4561 agctatggag tgactaaggc agcggttgaa ctggcagcta actacgatac aaccatcatg
     4621 accatgattc ggccacgtgg tggtgacttt gtctataatg acctagaaat tgctatcatg
     4681 ctagaagaca ttcgtttgac tgctcaggct ggaagtcaag gggttgtatt tggagcttta
     4741 actgctgata aaaagttgga taagcctaat ctggaaaagt taattgctgc atcaaaagga
     4801 atggaaattg tctttcacat ggcctttgat gaactaagtg atgaagatca accggaagct
     4861 attgactggc tcagtcaagc cggtgtcact cgtatcctaa ctcgtgctgg tgtgtctggc
     4921 gactccttag aaaaacgttt tgttcactat cacagaattt tgaagtacgc taaaggtaaa
     4981 attgaaattc taccaggtgg ggggattgac cttgaaaacc gtcaaacctt tatcgaccag
     5041 gtgggggtaa cacaattgca tggtactaag gttgtttttt aaaaaataga aaggaactgc
     5101 tagctttggg tagcagtttt cacttatgtt tgaaattttt aaatcctatc aatttaatca
     5161 agaaaaggct catgattatg gttttataga aaatagcgaa gtctggacat atagttgcca
     5221 gattttgcaa ggtgactttg tcatgactgt gtccatcact gctgataatg tgaactttca
     5281 agtctttgac caagagactg gtgacctcta tcctcacgtt tatatggaaa gcatgagggg
     5341 aagttttgtc ggaaatgtcc gtgaggcttg tctggagatt ctttaccaga ttcggaaggc
     5401 ttgttttgat gtgcaagatt ttatctgtca tcagactaag cgtatcatga ctcaagttca
     5461 ggaaaagtat ggaaaccagt tggagtatct gtgggaaaaa tcgcctgata cagctgtatt
     5521 gcgccatgaa ggcaatcaaa agtggtatgc cgtcttgatg aaaatctctt ggaataagct
     5581 ggaaaagggc agagaaggac aagtggaagc agtcaacctc aagcatgacc aagtagctaa
     5641 tttgctttca caaaagggga tttatccagc cttccatatg agcaagcgct actggattag
     5701 tgtgtccctt gatgatactt tatcagatga agaagtactg gaattgatag aaaaaagttg
     5761 gaacttaacc tctaaaaaat gaaatatttt aataattttc atgaactttc aattagctaa
     5821 atattcttta ctgaagaaat tttcagaaaa tataggattt accacaccag aggaatgtgg
     5881 tgccatcttc aaatacctga ttgagaatgt caagacggat cgtcagatca tctattcgcc
     5941 tcactgtcat gatgacctcg gaatggcagt ggcaaatagc cttgctgctg tcaagaatgg
     6001 tgcaggacgt gttgaaggga ctatcaatgg tattagggag cgagctgaaa atgctgcttt
     6061 ggaagaaatt gcagtggctc tcaatattcg ccaagattac taccaagtag aaaccagtat
     6121 tgtcctaaat gagaccatca atacgtcaga aatggtttct cgcttctctg gtattccagt
     6181 tcctaaaaac aaagccgtcg ttggtggcaa taccttctcc cacgaatctg gtattcacca
     6241 agatggagtc cttaaaaatc ctctcactta tgagatcatc acacctgaat tggttggtgt
     6301 taagattccg cttggaaaat tatctggtcg ccatgctttt gttgagaaac tgagagaatt
     6361 ggccctagat tttacagaag aggatatcaa accactcttt gctaagttca aggcactggc
     6421 cgataagaag taagaaatca cagatgcaga tactcgagct ttggtagctg gaaccatggt
     6481 tgaaaatcca gaaggcttcc actttgatga tttacaactt caaactcatg cagataatga
     6541 cattgaagcg ctcgttagcc tagccaatat ggatggtgag aaagtcgaat ttaatgcgac
     6601 agggcaaggt tccgttgaag caatctttaa cgctatcgat aagttcttta accaatctgt
     6661 tcgtttggtg tcctacacta tcaatgcggt aacagatgga atcgatgccc aggatcgggt
     6721 tttggtcact gttgaaaaca gagatacaga aaccatcttt aatgcagcag ggcttgattt
     6781 tgatgtgttg aaggcttctg ctattgtcta tataaacgct aatacctttg ttcaaaaaga
     6841 gaatgcaggt gagatgggac gcagtgtttc ttaccacgat atgcctagtg tgtaaaggag
     6901 aaggctatgg caaagaaaat agtagctcta gcaggagacg gaattggccc agaaatcatg
     6961 gaggctggtt tagaagttct ggaggctcta gctgaaaaaa caggttttga ctatgagatt
     7021 gacagacgac cgttcggagg tgcagatatt gatgcagcag ggcctccctt acctgatgaa
     7081 acccttaagg caagtaggga agcagatgct atcctactag cagctatcgg tagtcctcag
     7141 tatgatggag cagtggttcg ccctgaacaa ggcctgatgg ctctccgtaa ggaactcaat
     7201 ctttacgcta atattcgtcc tgtaaaaatc tttgacagtc tcaagcattt gtcaccactc
     7261 aaactggaac gaattgctgg tgtagacttt gtcgtggtgc gtgaattgac aggcgggatt
     7321 tactttggag attatattct tgaagagcgc aatgcgcgtg atatcaacga ctatagctat
     7381 gaggaagtgg agcggattat tcgcaaagcc tttgaaattg caagaaatcg cagaaaaatc
     7441 gttactagta tcgataagca aaatgttcta gcgacctcaa aactctggcg gaaagtagct
     7501 gaggaagtcg cacaggattt cccagatgta accttggaac accagctggt agactcagct
     7561 gctatgctta tgattaccaa tcctgctaag tttgatgtta ttgtaacgga gaatcttttt
     7621 ggagatattt tatctgatga atcaagcgtc ttatctggta cacttgaggt tatgccatca
     7681 gccagtcatt ctgaaaatgg accaagtctc tatgaaccta ttcacggttc agcacctgat
     7741 attgcaggtc aaggaattgc caatcctact tccatgattt tatcagttgc catgatgttg
     7801 agagatagtt tcggacgtta tgaggatgca gagcgtatca aacgtgctgt tgagacaagt
     7861 ctggcggcag gaattttaac gagagatata ggaggtcagg cttcaacaaa ggaaatgacg
     7921 gaagctatta ttgcaaggtt atgaagttag acgaaaaaat tgctctagtc cttttgattt
     7981 ggaatgtcat cattttcttg atttatggca ttgacaaatt caaggcaagg agaagagctt
     8041 ggcgcatcca agagaaaatc ttactcattt tagcccttat ttgtggtggt tttggggcct
     8101 ggttagctgg aatcactttt caccacaaga ctcgaaaatg gtactttaaa acagtttggt
     8161 ttcttgggat ggtgaccaca ctagtagcct tatattttat ttggaggtaa tggatggcag
     8221 ggtcttcgag ggaacacgct gcttgggttc tagcggacta tggttttaag gtcgtgattg
     8281 caggatcttt cggtgacatt cattacaata atgaactcaa taatggcctg ttgccaatcg
     8341 ttcagcctag agaggttaga gagaaattag cccagctaaa accaatcgac caggcaactg
     8401 tggacttgga acaacaaaaa atcatctcac cagttgaaga attcaccttc gagatagata
     8461 gcgagtggaa acataaactc ctaaatactt tggatgatat cggtattacc ttgcagtatg
     8521 aagagttgat tgctgcttat gaaaaacaac gaccagccta ctggcaggat tagaaaaaat
     8581 agaaaaggaa atatagtaaa ctgaaataag atgtaaacaa atgaattaga gcttaacatc
     8641 catttccagc aattttttag aaactacagt ggactattct ggattcaaca cattataaaa
     8701 ttatgacaaa acacattcac aagaaggcta cgacatttta aaaggtgagg gcggatgtat
     8761 cgtttgccct actaaagttg gttacattat catgaccagt gacaaggcag gacttgagcg
     8821 taagttcgca gccaaagaac gtaagcgtaa caaaccaggt gttgttctct gcggtagcat
     8881 ggatgaactt cgcgctttag cgcaactcaa cccagaaatt gaagcattct actaaaaaca
     8941 ttgggatgaa gatattcttc ttggttgtat ccttccttgg aaaccagaag cctctgaaaa
     9001 actcaaagca tacggggatg gccgtgaaga acttatgact gatgtacgtg gtactagctg
     9061 ttttgttatc aagtttggaa aagcaggtga acaattggct gccaagcttt gggaagaagg
     9121 taaaatggtc tacgcctcat ctgcttcaat gacaaaacga ttgaaactcg ctatgagagc
     9181 aaggtgtaat ggtgtctatg gtcgataagg acggcaaact catcccagaa caaggaggag
     9241 cacgttcaac ttcaccagca ccagttgtga tccgtaaagg gcttgacatt gatatagtaa
     9301 aatgaagtaa gagcaggaca aattaatcag gacagtcaaa tcgatttcta acaatgtttt
     9361 agaagtagag gtgtactatt ctagtttcaa tccactataa aatcatgatg cacctgtcag
     9421 atacttttaa ctcatgggac taccgtcagg ttgagtatta ttaggataga gaagaagtct
     9481 agtgttaagg acattacagc tcataatact aggctttttt atagaaattc ttttcttttt
     9541 ctataggata tggtattcta tgtagaaaat atatgttaat aagtaatgcc aatatttaaa
     9601 catcattagt aaaaggagtt agattgatga ataaaagaaa agttagttta gaagattttt
     9661 ataaatggta tagtctaaat aaagaagagt tattaaataa ggcaactgtt ggtgaaaagt
     9721 ttaatgataa attaaaagaa gagtttctcc aggaatggcc tttggatagg attttaacaa
     9781 tgtcaatcga tgaatatgta ataggaaagg gacagcaaaa taagtcttta tgctacgctc
     9841 ttgagaaggg aaaatacaaa aatctatttc ttggaatttc tggtggctca gcttcaaaat
     9901 ttggtattta ttggaataaa aaaacaaaca aatataaaga
//
LOCUS       DBERDK010000069         9768 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.069, whole genome shotgun sequence.
ACCESSION   DBERDK010000069 DBERDK010000000
VERSION     DBERDK010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 9768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9768
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.069"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            76..1335
                     /locus_tag="ACL73L_001698"
     CDS             76..1335
                     /locus_tag="ACL73L_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HGS0772635.1"
                     /translation="MKVFQHVNIVTCDQNFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIIDYQGAWIMPGLVNCHTHSAMTGLRGIRDDSNLHEWLNDYIWPAESEFTPDMTT
                     NAVKEALTEMLQSGTTTFNDMYNPSGVDIQQIYQVVKTSKMRCYFSPTLFSSETETTA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHVHVAE
                     TKEESGIILKRYGKRPLAFLEELGYLDHPSVFAHGVELNEREIERLASSQVAIAHNPI
                     SNLKLASGIAPIIQLQKAGVAVGIATDSVASNNNLDMFEEGRTAALLQKMKSGDASQF
                     PIETALKVLTIEGAKVLGMENQIGSLEVGKQADFLVIQPQGKIHLQPQENMLSHLVYA
                     VKSSDVDDVYIAGEQVVKQGQVLTVEL"
     misc_feature    1411..1546
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            1585..2085
                     /gene="rplJ"
                     /locus_tag="ACL73L_001699"
     CDS             1585..2085
                     /gene="rplJ"
                     /locus_tag="ACL73L_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HGS0772636.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNDFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     gene            2161..2529
                     /gene="rplL"
                     /locus_tag="ACL73L_001700"
     CDS             2161..2529
                     /gene="rplL"
                     /locus_tag="ACL73L_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HGS0772637.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DAADAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKAKLEEAGASVTLK"
     gene            2716..2886
                     /locus_tag="ACL73L_001701"
     CDS             2716..2886
                     /locus_tag="ACL73L_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772638.1"
                     /translation="MQKEGQSSRQFVKSHLMTIMGVDIVKATQLVDEMEQVGLIRFDE
                     FGNVGILVLEGQ"
     gene            2886..3134
                     /locus_tag="ACL73L_001702"
                     /pseudo
     CDS             2886..3134
                     /locus_tag="ACL73L_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            3148..3488
                     /locus_tag="ACL73L_001703"
                     /pseudo
     CDS             3148..3488
                     /locus_tag="ACL73L_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3481..3861
                     /locus_tag="ACL73L_001704"
     CDS             3481..3861
                     /locus_tag="ACL73L_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HGS0772639.1"
                     /translation="MIDLYLSKNRQRNQLLLDFFQNYGIEVSCHSVSEMTKDKLIEMM
                     SYSSDCFEFLSPNLLRFKNRDNLRLTDFVEIILKNPELTIRLPLAVSNKRVYPSLNLE
                     EARALLPRDTKQLIYMAQTHYLSN"
     gene            3874..4107
                     /locus_tag="ACL73L_001705"
                     /pseudo
     CDS             3874..4107
                     /locus_tag="ACL73L_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840775.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            4110..4697
                     /locus_tag="ACL73L_001706"
     CDS             4110..4697
                     /locus_tag="ACL73L_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HGS0772640.1"
                     /translation="MKRIIPVYIFQQVNVLLVSLYLLKFLCIGELTILQILYGSSLIS
                     FLWMYGQRKQAHKVNMKSRMKWLGVEFVSLLIISLCFSLIHAQGSTNQANLIGLQHQI
                     PWFSFLLFLINASMVEEFLYREILWNLVRKLDIRVALTSVLFALAHHTGTILAWCLYV
                     SLGMFLGMVRYKSDLWGSMGLHLVWNLLVYSLLLF"
     gene            4899..5036
                     /locus_tag="ACL73L_001707"
     CDS             4899..5036
                     /locus_tag="ACL73L_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapB/AmfS family lantipeptide"
                     /protein_id="HGS0772641.1"
                     /translation="MAEEVLNLQLVSVQVDETDEVDGMRFSTFSTNRCGNWSAFSWEN
                     C"
     gene            5215..7824
                     /locus_tag="ACL73L_001708"
     CDS             5215..7824
                     /locus_tag="ACL73L_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase LanC family protein"
                     /protein_id="HGS0772642.1"
                     /translation="MDYNFNLEHPFFFTNNDYSTDTSIKYQVSLPFNWHEVMNNDEWV
                     YQYPIGKFVERQGWKIHISSEYNSSHELLQDVAKICHEMRIPFKHLSTEDKFIMRNGK
                     LVSRGFSGKFITCYPNQNELESVLQRLESALKQYNGPYILSDKRWDEAPIYLRYGVFR
                     PSRDDEKKVAIDELIVGDEVVKDERLPVFKIPKGIVPPDFLNKWLDKKDKKQGDFPFI
                     IDNAIRFSNSGGIYNARLKEDGKKIILKEARPYTGLGFDGTYSSEKLASECKALKILN
                     EWSETPKIYWHGKIWEHTFLGIEHMKGVPLNRWVTNNFPLYEVVDKTKDYLLRVSKIV
                     EKLIDLTNKFHSENVYHQDLHLGNILVKDEDEISIIDWEQAVFSNDEKVVHKVAAPGF
                     RAWRETLPSEIDWYGIRQIAHYLYMPLVTTSDLTYNYVSQTRIEGKKLFESLGYTREH
                     IDYVESLLSYLDSKCPQIENISRKKVLKPMHEIRTIESEQDIQDFIIKLLRGFTLTYG
                     QWRKEFQSRFFPVHYYGLNFNQGIAFSDLAILWSYQQLAKKVKNFKFDDYYEIRTQVI
                     NEAVNNFKKSSLSGLFDGKIGTIWLIYEFGEIDRAVELFTTHFIEIFENSQNKNLYSG
                     QAGILLVGLYFLSKGEIDNKLGEEILIRLREYTLNYIENPETFCKVGASDVQSNDPYE
                     NFGGLLYGHAGVAWLFGEAYKLTGESIYKNGLELAVDKELVAYKVDSNNSLQYSQGHR
                     LLPYLATGSAGLLLLINRNKEILSSKYLKYLTSLERATDVVFCVLPGLFNGFCGLEVA
                     NNIYSDIDDNFSGQKKLIEQLYRYLCVIEEGFVIAGDNGLKITTDIASGFAGVAIGLV
                     SIMDNKLTILPQI"
     gene            7857..>9768
                     /locus_tag="ACL73L_001709"
     CDS             7857..>9768
                     /locus_tag="ACL73L_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HGS0772643.1"
                     /translation="MNQSNEIFLNTIQEKTHYKRSISSSFQELKKWNSLIFRRGRYWY
                     QFDNWSIPNTGVIKEWYVEDNDWIPKVSSSSLDNSNSLKIVFDVEKYTLPDDSILQDV
                     RISSDESYIVLVVSSLRSTNLIGIKKYTMEELFIIEDISVESSFYLGKFGVMYTRSKE
                     YGRPSKLFYKSFDSFTEEELFEENECSFRLKIVHIDSNNCFVKSVDFQKGRIFLYSFD
                     RTGFVRHSYTETVAPTPRDIALFSTEKAQYFLGLSSTEEKKDQLILRETSSGDRVSIT
                     IPYQDRARRVHCIGRYILLDCSNAANSVFYLATFKNNSLRELSVNKITFDEKTTLYEN
                     SFSDRVLLFLERRTFYEKILSFDLIENTLKTEFERPIIKSNNTKYFSKVIWTKKDSYD
                     VSIPISLFWKSEDTDELPRRKKCILSVYGAYGKNDNSDLDEIMLSIIDAGFIYAIVHV
                     RGGGYLGGEWYRSGKALNKWNSIRDFIEGVNYLRENDVIDSKRLGLITSSAGGIIAGA
                     VLNEEKNLLQSILLFSPFINPYDTLQNPNDPLSKTEIAEWGDIRDPEVKAYIKSYSPM
                     QNIEKARDSNTVIVNILGEKDPYINNNEVIEWSKKLNSIGVKSLLYLNKAAGHGGFTP
                     SDVLLMIDTLNYF"
BASE COUNT         3243 a         1434 c         2021 g         3070 t
ORIGIN      
        1 aaccacacct acggcaaagt gaatctgaag tggtttgaag agagtacaac ttgtctttta
       61 gaaaaggagc ctacaatgaa agtctttcag catgtaaata tcgtgacttg tgatcaaaat
      121 ttccatgttt atcttgatgg aatcttagca gtcaaggatt ctcaaatcgt ctatgtcggt
      181 caagataagc cagcgttttt agaacaagct gagcagatta tagactatca gggagcttgg
      241 attatgcctg gtttggtcaa ttgtcacacc cattctgcaa tgacaggtct gagagggatc
      301 cgagatgaca gcaatctcca tgaatggctc aatgactata tctggccagc agaatctgag
      361 tttactcccg acatgactac caatgcggtc aaagaagcct tgacagagat gctccagtca
      421 ggaacaacaa cctttaacga tatgtataat cccagtggtg tggatatcca gcaaatttat
      481 caggtggtga aaacttccaa gatgcgttgt tatttttctc cgactctctt ttcttcagag
      541 acagaaacaa ctgctgagac tataagcaga actcgatcca tcatagatga aatcttaaaa
      601 tataaaaatc caaatttcaa ggttatggta gcacctcatt ctccgtatag ctgcagtaga
      661 gacttgctgg aagcgagttt ggaaatggca aaagagctaa atattccgct ccatgtccat
      721 gtggcggaga ccaaggagga gtcaggaatt atcctcaaac gatacggcaa acgccccctt
      781 gcttttcttg aagaactggg ttatttagat catccgtccg tatttgctca cggggtcgaa
      841 ttaaacgaga gagaaattga acgcttggca tcttctcaag tggctatcgc ccacaatcct
      901 atcagtaacc tcaaactggc atcaggaatt gctccaatta tccagctcca aaaagcggga
      961 gtagcagtcg gaattgcgac tgactcggtt gcttccaata acaatctaga tatgtttgag
     1021 gaaggaagga ctgcagctct tcttcagaag atgaaaagtg gggatgccag ccagtttcca
     1081 atcgaaacag ctctcaaggt actgacaatc gaaggggcta aggtccttgg aatggaaaat
     1141 cagataggaa gtctggaagt cggcaagcaa gcagattttc tggtcattca accacaaggg
     1201 aaaattcatc tccaacctca ggaaaatatg ctgtctcacc tggtttatgc agttaaatct
     1261 agtgatgtag atgatgttta tatcgccgga gaacaggttg ttaagcaagg tcaagtcctg
     1321 acagtagaac tttaaaagaa aaattacgaa aaattttaaa aaaagtttgc aaaaatcttg
     1381 cattcttttt ttgactatgc tatacttata tacggtttaa aaaaactgcc taagacagta
     1441 ggggagctcg actcataaat atcctaccga ggacaaaacg tatcatgtaa aaagaagcgt
     1501 attgtacttt cgtgtctagg tttgggcgcg tttttctttt tgaaaaattc cccaagcaaa
     1561 ataattacgg aggtgaacac actaatgagt gaagcaatta ttgctaaaaa agcggaacta
     1621 gttgacgtag tagctgaaaa aatgaaagct gctgcatcta tcgtcgttgt agacgctcgt
     1681 ggtttgacag ttgagcaaga tacagttctt cgtcgtgagc ttcgtggaag cgaagttgag
     1741 tataaagtta ttaaaaactc aatcttgcgt cgtgcagctg aaaaagctgg tcttgaagat
     1801 cttgcatctg tatttgttgg accatctgca gtagcatttt ctaatgaaga tgttatcgca
     1861 ccagcgaaaa tcttgaacga cttttctaaa aacgctgaag cacttgaaat taaaggtggt
     1921 gcaatcgaag gcgctgtcgc atctaaagaa gagattcttg cacttgcaac tcttccaaac
     1981 cgcgaaggac ttctttctat gctcctttct gtacttcaag cgccagtgcg caacgttgct
     2041 cttgcagtca aagcggttgc agaaagcaaa gaagacgcgg cttaatctta agctacacag
     2101 cgtagcctag ctacgaaaaa aactattata aaatttaaaa cttatttgga ggaaataaca
     2161 atggcattga acattgaaaa cattattgct gaaattaaag aagcttcaat ccttgaattg
     2221 aacgaccttg taaaagctat cgaagaagaa tttggtgtaa ctgcagctgc tcctgtagct
     2281 gttgctgcag ctgatgcagc tgatgctggt gctgctaaag attcattcga cgttgaattg
     2341 acatctgcag gcgacaaaaa agttggcgtt atcaaagttg tacgtgaaat cactggtctt
     2401 ggtcttaaag aagctaaaga acttgttgac ggtgcaccag cacttgttaa agaaggcgtt
     2461 gcaactgcag aagctgaaga aatcaaagct aaattggaag aagctggagc ttcagttact
     2521 cttaaataat agagtatcgc aatttgattg cggaatccct atttaccagt ctttgagagc
     2581 gataagcgat tgatagaaac tgataaattg atttggtttc ctgccaaaaa ttctttggca
     2641 ggatttttgt tttaataaac tttcgggggt agacactaga ttgtctaccc ctttttttgt
     2701 gtttcggagg taattatgca aaaggaagga cagtcatcac gtcaattcgt gaagagtcat
     2761 ctcatgacta tcatgggagt tgatattgta aaagcaactc agttagttga tgaaatggaa
     2821 caagtaggtt taatccgatt tgatgaattt ggaaatgttg gaattttagt cttggaggga
     2881 caataatgaa gcgtattacc gctaatcaat atcagacatc agagcgttat tataagctcc
     2941 ccaaactttt atttgagagt gaacggtata agaatatgaa actggaggtt aaggtggttt
     3001 actcagtttt aaaagatcgg ttggagttgt ctttgagcaa aggttggatt gatgaggatg
     3061 gggctattta tttgatttat tccaattcaa atttgatggc acttttaggc tgttcaaagt
     3121 caaaattact ctccatgtga gtttgaagtg acatttttag atgattacca taaaaaacat
     3181 aactacccac tattttacga atcctatctt caaaacgtta tggaattcct tgaaagtcaa
     3241 gacataaaga atggggttga tgcctttgta gatgatcatc aaaatctcgt ttttgtttta
     3301 tatggacaag gctatcgagc cgagggaaaa gagggaatac ttacaaccca agtaactgta
     3361 aaagcttatg atgaagacaa gaaaccgatt aacttcgcaa atttattaga ttccttaatt
     3421 tattagattc cttaatcgtg tcagaatatc aaatggaacc gaatctttgg gaggtctccc
     3481 atgattgatc tctatctaag taaaaataga caaagaaatc aacttctttt agatttcttc
     3541 caaaactatg gcatagaggt atcttgtcat tcagtttctg aaatgacaaa ggacaaatta
     3601 attgagatga tgagctattc ttcagattgt tttgagtttt tatctcccaa tttattacga
     3661 tttaagaacc gtgacaatct aagattaacg gatttcgttg agataatttt aaaaaatcct
     3721 gaactaacca tcagacttcc tcttgcggtt tcaaataagc gagtttatcc aagtctgaat
     3781 ttggaagagg ctagagctct attgccaaga gatacgaaac aattgattta catggcgcaa
     3841 acacattatt tatctaacta aaggagaagc aatatggctg aacgattttg ggagaacttg
     3901 tccattattt tggctgaaag aaatatcagc tggattgagt taaccagaaa aatgtttgcg
     3961 ggagagtttc actatccaag tgaattgaat cgtttgtatc aaaagattcg ccactagaag
     4021 atggaacaat gaatgcctca aagtccatgg gtagaaagga ttgtgcaggt attagattta
     4081 gattatgaag atttatttcg gaggtaacta tgaaaagaat aattccagtt tatatattcc
     4141 aacaagtaaa tgtcctattg gtatctctat acttattgaa atttctttgt atcggtgagt
     4201 taactatact acagattctc tatggttcgt cactcatttc ttttttatgg atgtatggcc
     4261 aacgcaaaca agcgcataag gtcaatatga aatctaggat gaaatggctt ggtgttgaat
     4321 ttgttagcct actgattata agtctatgtt ttagtctgat tcatgctcaa ggaagcacga
     4381 atcaagcaaa cttaattggc cttcaacatc aaattccttg gttttcattt ttattgttct
     4441 taatcaatgc gagtatggtt gaagaatttc tgtatcgaga aattttatgg aatttggtca
     4501 gaaaattaga tatcagagtt gctttgacaa gtgttttatt tgccttagca catcatacag
     4561 gaaccattct agcttggtgt ttgtatgttt cacttgggat gtttttaggt atggtgcgct
     4621 ataaatctga cttatggggc agtatgggac tacatttggt gtggaaccta ctagtttata
     4681 gtttgctgct tttttaaaat aggtgacctg atttttaggt aacttctaaa actaacttcc
     4741 agttttggga gagagatgga agttactttg aaaagttacg gaaacattat tttctatgtg
     4801 acaacattgt ataattgctt gcattttact ggtggagaga gtataatgag ggtggataaa
     4861 gtacttaatc ccaaaaattt aaataggagg agctagctat ggcagaagaa gtattaaatc
     4921 ttcaattagt atctgttcaa gttgacgaaa cagatgaagt tgatggaatg cgatttagta
     4981 cattcagtac aaatcgttgc ggtaactgga gtgcttttag ttgggaaaat tgttaattca
     5041 tttaaattgt aaactttaga gattctctgg agttatggat gtcgttccaa tatgtgcacg
     5101 ttggaatgtt agtgcttata tggggagaat ccaaaggatt ctcccttttt atttaaataa
     5161 gtctgtctag ctatagatat attaagtttg ataaaatttc ttggagattt tgttatggat
     5221 tataatttca atttggagca tcctttcttt ttcactaata atgattatag tacagatact
     5281 agcataaagt accaagtaag tctgcctttt aattggcacg aagtaatgaa taatgatgag
     5341 tgggtttatc aataccctat tggaaaattt gttgaaagac aagggtggaa aatacacatt
     5401 agttcagagt ataacagttc acatgaattg ttacaagatg tagcaaaaat ttgccatgaa
     5461 atgcgaattc catttaaaca tcttagtaca gaagacaaat ttatcatgcg aaacgggaaa
     5521 ttggttagtc gaggattttc tggtaaattt attacatgtt atccaaatca aaatgaatta
     5581 gagtcggttt tgcaaaggtt agaaagtgcc ttaaagcaat ataatgggcc atatatttta
     5641 agcgataaac gttgggatga agcaccaata tatctgaggt atggtgtgtt taggccgtca
     5701 agggatgatg aaaagaaggt tgctattgat gaattaattg ttggcgatga agttgttaaa
     5761 gatgaacgtt taccagtatt taaaattcca aaaggtatag tgccccctga ctttttgaat
     5821 aaatggcttg acaagaaaga taaaaaacag ggagactttc cctttattat agataatgca
     5881 attagatttt caaacagtgg tgggatatat aatgctaggc taaaagaaga tgggaaaaaa
     5941 ataattttaa aagaggctag accgtataca ggtctaggat ttgatggtac ttattcttca
     6001 gagaaattag cttcggagtg caaagcacta aaaattctaa atgaatggag tgagacgcct
     6061 aaaatatact ggcacggtaa gatctgggag cacaccttct taggaattga acatatgaag
     6121 ggcgttccac taaatagatg ggttaccaat aattttcctt tatatgaagt tgttgataag
     6181 accaaagatt atctattgag agtttcaaag atagtagaaa aactcatcga tttaacgaat
     6241 aagtttcatt ctgaaaatgt atatcatcaa gatttacatt taggaaatat cttggttaag
     6301 gatgaagatg agattagtat aatagactgg gaacaggctg tcttttcaaa tgatgaaaaa
     6361 gttgtacata aagttgcagc ccctggtttt agagcatggc gagaaacact tcccagtgag
     6421 atagattggt atggtattag acaaatagct cattatttat acatgccact agtaactaca
     6481 tcggatttga catacaacta tgtaagtcaa accaggatag aaggaaaaaa actatttgag
     6541 tctttgggtt atacaagaga acatatagat tatgtagaaa gtttattgtc atatctagat
     6601 tctaaatgtc cacagattga gaatatttcc aggaaaaaag ttttaaaacc catgcatgag
     6661 attagaacta tcgagagtga gcaagatata caagatttta ttataaaatt attgagaggg
     6721 tttacgctaa cttatggaca gtggcgtaaa gaatttcaat caagattctt cccagttcat
     6781 tattatggtc tgaattttaa tcaaggaatt gcgttttcag atttagctat cctttggagt
     6841 tatcaacaac tagctaagaa agtaaaaaat ttcaagtttg atgactatta tgagattcga
     6901 acacaggtta ttaatgaggc agtaaataat tttaagaaat cgtccctttc tggtcttttt
     6961 gatggtaaaa ttggtacaat ctggctcatt tatgaatttg gggagataga tcgggcagtt
     7021 gaacttttta caactcattt tatagagata tttgagaatt cacaaaataa aaatttatat
     7081 agtgggcagg ctggaatttt actggttgga ctgtattttc ttagtaaagg ggaaattgat
     7141 aataaactag gagaagaaat actaattagg cttagagaat atactttaaa ttatatagaa
     7201 aatccagaaa ctttttgtaa agttggcgct agtgatgttc agagtaatga tccttatgag
     7261 aattttggtg gattactata tggtcacgca ggggtagctt ggctcttcgg tgaagcttat
     7321 aaattaactg gtgaaagtat ctataaaaat ggtctagaat tggctgtaga taaagaatta
     7381 gttgcttata aagtagatag taataattca ttgcagtaca gccaaggtca tagattacta
     7441 ccatatctag caactggaag tgctggcctg cttttactta ttaatagaaa taaagaaatc
     7501 ttgtcaagca aatacttaaa atatttaact tctttagaga gggcaactga tgtagttttt
     7561 tgtgtattac caggtttatt taacggattt tgtggtcttg aagttgctaa taatatatat
     7621 agtgatatcg atgataactt ttcaggacaa aagaaattaa ttgaacaact ttatagatat
     7681 ttgtgtgtaa ttgaagaagg atttgttatt gcgggagata atggtttaaa gataacaaca
     7741 gatattgctt cgggatttgc gggtgttgca attggattgg tatcaataat ggataataaa
     7801 ttgacgattc ttcctcaaat ataatattta gataccatta ttaaggggtc atacttgtga
     7861 atcagagtaa tgaaatattt ttaaatacaa tacaggaaaa aacacactat aagaggtcta
     7921 tctcttcatc atttcaggaa ttaaaaaaat ggaattcttt gatatttagg agagggcgat
     7981 attggtacca atttgataat tggagtatac ccaatactgg tgtaataaag gaatggtatg
     8041 tcgaagataa tgactggatt cctaaggtta gttcttccag tttagacaat tcaaattcac
     8101 ttaaaattgt ctttgatgtg gaaaaatata ctttgccaga tgatagtatc ttacaggatg
     8161 ttaggatttc atctgatgag agttatatag ttttggttgt atcctcactg cgtagcacta
     8221 atttaattgg aataaaaaaa tatactatgg aagagttatt tatcattgaa gatatttcag
     8281 tagaaagttc gttctatttg ggtaaattcg gtgttatgta tactcgctcc aaagagtacg
     8341 ggaggccaag taagttattc tataaatctt ttgatagctt tacagaagag gaactgtttg
     8401 aagaaaacga atgctctttt cgattaaaaa tagtccacat tgatagcaat aattgttttg
     8461 taaagtcagt agactttcag aaaggcagga tttttctata cagtttcgac cggacgggtt
     8521 ttgttagaca ctcttacaca gaaacggtag ccccaacacc tagagatatc gctctattta
     8581 gtacagaaaa agcacagtac tttcttgggc tctcttcaac tgaagaaaag aaagatcaac
     8641 taattctaag ggaaacttct tcgggagaca gagtttctat tactatacca tatcaagata
     8701 gggctagaag agttcactgt ataggacgtt atatcctttt agactgttca aacgcagcga
     8761 attccgtctt ttatcttgct acatttaaaa ataatagttt gagggagttg agtgttaata
     8821 agataacatt tgatgaaaag acaacacttt atgaaaattc attttctgat cgtgttcttc
     8881 tgtttttaga gaggagaact ttttacgaaa aaatacttag ttttgattta atagaaaata
     8941 cattaaaaac tgaatttgaa agacccatca taaaatcgaa taatactaaa tatttttcta
     9001 aggtgatttg gactaaaaaa gacagttatg atgtaagcat accgatttct ttattttgga
     9061 aaagtgaaga tacggatgag ctcccaagaa gaaaaaaatg tattctaagt gtctatgggg
     9121 cttatggtaa gaatgataat tctgatttag atgaaattat gttatctata atagatgcag
     9181 gttttattta tgctatagtt catgtaagag gtggaggcta tttgggtggt gaatggtatc
     9241 gctcaggaaa ggcgttaaac aaatggaatt ctatcagaga ttttattgag ggagtaaatt
     9301 atttaaggga aaatgatgta attgacagta agcgattagg tttaataact tctagtgcag
     9361 gtggaataat tgctggtgcg gtgttgaacg aggaaaaaaa tttattgcaa agcattctct
     9421 tattttcgcc atttataaat ccttatgata cacttcagaa tccaaatgat cctctttcta
     9481 agaccgaaat agcagagtgg ggagatatta gagaccctga agtaaaggct tatataaagt
     9541 catattcgcc tatgcaaaat attgaaaagg cacgagactc aaatactgtt atagttaata
     9601 ttttgggtga gaaagaccca tatattaata ataacgaagt gatagagtgg tcaaagaaat
     9661 taaattctat tggagttaaa agtttgttgt atttaaacaa agcagctggt catggaggtt
     9721 ttactccatc agatgttctc ttaatgattg atactttaaa ttatttct
//
LOCUS       DBERDK010000070         9753 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.070, whole genome shotgun sequence.
ACCESSION   DBERDK010000070 DBERDK010000000
VERSION     DBERDK010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 9753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9753
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.070"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            32..6046
                     /gene="iga"
                     /locus_tag="ACL73L_001710"
     CDS             32..6046
                     /gene="iga"
                     /locus_tag="ACL73L_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin A1 protease"
                     /protein_id="HGS0772644.1"
                     /translation="MEKYFGEKQERFSFRKLSVGLVSATISSLFFMSVLASSSVDAQE
                     TAGVHYKYVADSELSSEEKKQLVYDIPTYVENDDETYYLVYKLNSQNQLAELPNTGSK
                     NERQALVAGASLAAMGILIFAVSKKKVKNKTVLHLVLVAGIGNGVLVSVHALENHLLL
                     NYNTDYELTSGEKLPLPKEISGYTYIGYIKEGKTTSESEVSNQKSSVATPTKQQKVDY
                     NVTPNFVDHPSTVQAIQEQTPVSSTKPTEVQVVEKPFSTELINPRKEEKQSSDSQEQL
                     AEHKNLETKKEEKISPKEKTGVNTLNPQDEVLSGQLNKPELLYREETIETKIDFQEEI
                     QENPDLAEGTVRVKQEGKLGKKVEIVRIFSVNKEEVSREIVSTSTTAPSPRIVEKGTK
                     KTQVIKEQPETGVEHKDVQSGAIVEPAIQPELPEAVVSDKGEPEVQPTLPEAVVTDKG
                     ETEVQPESPDTVVSDKGEPEQVAPLPEYKGNIEQVKPETPVEKTKEQGPEKTEEVPVK
                     PTEETPVNPNEGTTEGTSIQEAENPVQPAEESTTNSEKVSPDTSSKNTGEVSSNPSDS
                     TTSVGESNKPEHNDSKNENSEKTVEEVPVNPNEGTVEGTSNQETEKPVQPAEETQTNS
                     GKIANENTGEVSNKPSDSKPPVEESNQPEKNGTATKPENSGNTTSENGQTEPEPSNGN
                     STEDVSTESNTSNSNGNEEIKQENELDPDKKVEEPEKTLELRNVSDLELYSLSNGTYK
                     QHISLEQVPSNPNSYFVKVKSSSFKDVYLPVASISEERKNDKILYKITAKVEKLQQEI
                     ESRYKDNFTFYLAKKGTEETTNFTSFSNLVKAINQNPSGTYHLAASLNANEVELGPDE
                     RSYIKDTFTGRLIGEKDGKNYAIYNLKKPLFENLSGATVEKLSLKNVAISGKDDIGSL
                     ANEAQNNTKIKQVHVDGVLAGERGIGGLLAKAEQSSITESSFKGRIINTYETTAAYNI
                     GGMVGHLTGDKALLTKSKATVAISSNTNTSDQTVGGLAGLVDRDAQIQDSYAEGDINN
                     VKHFGRVAGVAGNLWDRTSGDVRHAGSLTNVLSDVNVTNGNAITGYHYNEMKVKDTFS
                     SKANRVYNVTLVKDEVVSKESFEERGTMLDASQIASKKAEINPLILPTVEPLSTSGKK
                     DSDFSKVAYYQAKRNLTYKNIEKLLPFYNKATIVKYGNLVNENSLLYQKELLSAVMMK
                     DNQVITDIVSNKQTANKLLLHYKDDLSEKLDLKYQNDFAKLAEYSLGNTGLLYTPNQF
                     LYDQTSIIKQVLPDLQKVDYHSEAIRKTLGISPNVKQTELYLEDQFAKTKQQLEDSLK
                     KLLSADAGLASANPVTEGYLVDKIKRNKEALLLGLTYLERWYNFSYGQVNVKDLVLYH
                     LDFFGKGNASPLDTLIELGKSGFNNLLAKNNVDTYGISLASQHGTTDLFSTLEHYRKV
                     FLPNTSNNDWFKSETKAYIVEEKSTIEEVKTKQGLAGTKYSIGVYDRITSATWKYRNM
                     VLPLLTLPERSVFVISTMSSLGFGAYDRYRSSDHKAGKALNDFVEENARETAKRQRDH
                     YDYWYRILDDNAREKLYRNILLYDAYKFGDDNTVGKATEVADFDNPNPAMQHFFGPVG
                     NKVGHNQHGAYATGDAVYYMGYRMLDKDGAITYTHEMTHDSDQDIYLGGYGRRSGLGP
                     EFFAKGLLQAPDHPDDATITINSILKHSKSDSTESRRLQVLDPTTRFNNADDLRQYVH
                     NMFDVVYMLEYLEGNSILKLDTNQKQQLLRKVTNEYHPDPDGNKVYATNVVRNLTVEE
                     VERLRSFNDLIDNNILSSREYASGKYERNGYFTIKLFAPIYAALSNDIGTPGDLMGRR
                     IAYELLAAKGFKDGMVPYISNQYEEEAKQKGKTINLYGKTRGLVTDDLVLEKVFNNQY
                     HTWSEFKKAMYQERQDQFDRLNKVTFNDTTQPWQTFAKKTTSSVDELQKLMDVAVRKD
                     AEHNYYHWNNYNPDIDSEVHKLKRAIFKAYLDQTNDFRSSIFENKK"
     gene            6143..6274
                     /locus_tag="ACL73L_001711"
     CDS             6143..6274
                     /locus_tag="ACL73L_001711"
                     /inference="COORDINATES: protein motif:HMM:NF012948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HGS0772645.1"
                     /translation="MYQQETLPATGSSTSELTALGLLAVGSLVLLVHNMTGTVFCSL"
     gene            complement(6238..6423)
                     /locus_tag="ACL73L_001712"
     CDS             complement(6238..6423)
                     /locus_tag="ACL73L_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772646.1"
                     /translation="MMSKQFLFYDIEIYFILYHAFFILSDDSLLPYLLPYLLPYIEKS
                     HQMMTFQREQKTVPVIL"
     gene            6505..7356
                     /gene="ylqF"
                     /locus_tag="ACL73L_001713"
     CDS             6505..7356
                     /gene="ylqF"
                     /locus_tag="ACL73L_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HGS0772647.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLLILNKADLADPAMTKEWRQYFESQGIQTLAINSKEQVTVKVVTDAAK
                     KLMADKIARQKERGIQIETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGINYFKEHY
                     PEKLAERFKQMKIEEEAPVIIMDMTRALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EDLDGND"
     gene            7343..8122
                     /locus_tag="ACL73L_001714"
     CDS             7343..8122
                     /locus_tag="ACL73L_001714"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HGS0772648.1"
                     /translation="MATIKEIKELLVTVKELESPIFLELEKDNRSGVQKEISKRKRAI
                     QAELDENLRLESMLSYEKELYKQGLTLIAGIDEVGRGPLAGPVVAAAVILPKNCKIKG
                     LNDSKKIPKKKHLEIFQAVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
                     QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFSGYDF
                     ATNAGYGTAKHLEGLTKLGVTPIHRTSFEPVKSLVLGKKES"
     gene            8138..9688
                     /locus_tag="ACL73L_001715"
     CDS             8138..9688
                     /locus_tag="ACL73L_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HGS0772649.1"
                     /translation="MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRF
                     LIEKGFHLIQGVYQDQGYLVRNLFVLVLFYILICWLSAKLTRSEKDIKGSGIPQVEAE
                     LKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGIAKWLKSSPVEE
                     RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFG
                     LTPVLDMPDNIPPMTLDQYWIYLVMGIFLGFSGFLYEKAVLNVGRVYDLIGQKIHLDR
                     AYYPILAFILIIPVGIFLPQIIGGGNQLVLSLTEQNFSFQVLLAYFLIRFIWSMISYG
                     SGLPGGIFLPILALGSLLGALVGVICVNLGLVSQEQFPIFVILGMSGYFGAISKAPLT
                     AMILVTEMVGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSSEGEVTL
                     IEIPVSDKIAGKQVHELNLPHNVLITTQVHNGKSQTVNGSTRMYLGDMIHLVIPKSEI
                     GKVKDLLL"
BASE COUNT         3345 a         1800 c         1983 g         2625 t
ORIGIN      
        1 acatatataa attttatttc aaggaggaat aatggaaaag tattttggtg aaaaacaaga
       61 gcgtttttca tttagaaaat tatcagtagg acttgtatct gcaacgattt caagtttatt
      121 ttttatgtct gtattagcta gttcatctgt ggatgctcaa gaaactgcgg gagttcacta
      181 taaatatgtg gcagattcag agctatcatc agaagaaaag aagcagcttg tctatgatat
      241 tccgacatac gtggagaatg atgatgaaac ttattatctt gtttataagt taaattctca
      301 aaatcaactg gcggaattgc caaatactgg aagcaagaat gagaggcaag ccctagttgc
      361 tggtgctagc ttagctgcta tgggaatttt aatttttgct gtttccaaga aaaaggttaa
      421 gaataaaacg gtattacatt tagtattggt tgcagggata ggaaatggtg tcttagtttc
      481 agtccatgct ttagaaaatc atcttttgct aaattacaat acggactatg aattgacctc
      541 tggagaaaaa ttacctcttc ctaaagagat ttcaggttac acttatattg gatatatcaa
      601 agagggaaaa acgacttctg agtctgaagt aagtaatcaa aagagttcag ttgccactcc
      661 tacaaaacaa caaaaggtgg attataatgt tacaccgaat tttgtagacc atccatcaac
      721 agtacaagct attcaggaac aaacacctgt ttcttcaact aagccgacag aagttcaagt
      781 agttgaaaaa cctttctcta ctgaattaat caatccaaga aaagaagaga aacaatcttc
      841 agattctcaa gaacaattag ccgaacataa gaatctagaa acgaagaaag aggagaagat
      901 ttctccaaaa gaaaagactg gggtaaatac attaaatcca caggatgaag ttttatcagg
      961 tcaattgaac aaacctgaac tcttatatcg tgaggaaact atagagacaa aaatagattt
     1021 tcaagaagaa attcaagaaa atcctgattt agctgaagga actgtaagag taaaacaaga
     1081 aggtaaatta ggtaagaaag ttgaaatcgt cagaatattc tctgtaaaca aggaagaagt
     1141 ttcgcgagaa attgtttcaa cttcaacgac tgcgcctagt ccaagaatag tcgaaaaagg
     1201 tactaaaaaa actcaagtta taaaggaaca acctgagact ggtgtagaac ataaggacgt
     1261 acagtctgga gctattgttg aacccgcaat tcagcctgag ttgcccgaag ctgtagtaag
     1321 tgacaaaggc gaaccagaag ttcaacctac attacccgaa gcagttgtga ccgacaaagg
     1381 tgagactgag gttcaaccag agtcgccaga tactgtggta agtgataaag gtgaaccaga
     1441 gcaggtagca ccgcttccag aatataaggg taatattgag caagtaaaac ctgaaactcc
     1501 ggttgagaag accaaagaac aaggtccaga aaaaactgaa gaagttccag taaaaccaac
     1561 agaagaaaca ccagtaaatc caaatgaagg tactacagaa ggaacctcaa ttcaagaagc
     1621 agaaaatcca gttcaacctg cagaagaatc aacaacgaat tcagagaaag tatcaccaga
     1681 tacatctagc aaaaatactg gggaagtgtc cagtaatcct agtgattcga caacctcagt
     1741 tggagaatca aataaaccag aacataatga ctctaaaaat gaaaattcag aaaaaactgt
     1801 agaagaagtt ccagtaaatc caaatgaagg cacagtagaa ggtacctcaa atcaagaaac
     1861 agaaaaacca gttcaacctg cagaagaaac acaaacaaac tctgggaaaa tagctaacga
     1921 aaatactgga gaagtatcca ataaacctag tgattcaaaa ccaccagttg aagaatcaaa
     1981 tcaaccagaa aaaaacggaa ctgcaacaaa accagaaaat tcaggtaata caacatcaga
     2041 gaatggacaa acagaaccag aaccatcaaa cggaaattca actgaggatg tttcaaccga
     2101 atcaaacaca tccaattcaa atggaaacga agaaattaaa caagaaaatg aactagaccc
     2161 tgataaaaag gtagaagaac cagagaaaac acttgaatta agaaatgttt ccgacctaga
     2221 gttatacagt ttgtcaaatg gtacttataa acaacacatt tcgttagagc aagttccaag
     2281 caatccaaat agctactttg ttaaagtgaa atcttcttca ttcaaagatg tatacctacc
     2341 agtagcatca atatcagagg aaagaaaaaa tgataaaatc ctttataaaa tcacagcaaa
     2401 agtagagaag cttcagcagg agatagaaag cagatataaa gataatttta ccttctatct
     2461 agctaagaag ggaacagaag aaacaacaaa ctttacttcc tttagtaatc tggtcaaagc
     2521 tataaaccaa aatccctctg gaacctatca tttagcggcc agcctgaatg ctaacgaagt
     2581 ggagcttggt cctgatgaaa gatcctatat caaggacacc tttactggtc gtttaatcgg
     2641 tgaaaaagat ggcaagaatt atgctatcta taatttgaaa aaacctctgt ttgaaaactt
     2701 gagtggtgct acagtagaaa aactgagtct aaaaaatgtt gctatttcag ggaaagatga
     2761 tatcggttca ctggcaaatg aagctcagaa taacacaaaa attaagcaag ttcacgtcga
     2821 tggtgttctg gctggtgaac gtggtatcgg tggtttgctg gctaaggctg agcaatcaag
     2881 catcacagag agcagtttca agggaagaat tatcaacact tatgaaacga ctgctgccta
     2941 caatatcggt ggtatggtcg gtcatttgac aggtgacaag gctttactta ctaagtcaaa
     3001 agcgacagta gccatttcat ctaacacaaa tacttcagat cagactgtgg gtggacttgc
     3061 aggcctagta gaccgagatg cacagatcca agatagctat gctgaaggtg atatcaacaa
     3121 tgtcaagcac tttggtagag tcgctggagt ggcaggcaat ttgtgggatc gaacttctgg
     3181 tgatgttagg catgctggaa gtttgaccaa tgttctcagc gatgttaatg taaccaacgg
     3241 aaatgccatc actggttacc actataacga aatgaaggta aaggacacat tcagcagcaa
     3301 ggccaacaga gtctacaatg tcaccttggt caaggatgag gtcgtcagca aggaatcctt
     3361 tgaagaaaga ggaacaatgc tagatgcttc tcaaattgca agcaaaaaag cagaaatcaa
     3421 tcctctcatt ctaccaacag tggagccact ttcaacaagt ggcaaaaaag acagtgattt
     3481 ttctaaggtg gcctattatc aagctaagcg caacttgact tataaaaaca ttgaaaaatt
     3541 gctacctttc tacaacaagg caaccatcgt caaatacgga aacctggtca atgagaacag
     3601 tcttttatat caaaaagaac tcttgtcagc agtcatgatg aaggacaacc aagtcatcac
     3661 agacattgtt tctaacaaac agactgcaaa caaactcttg cttcactaca aggatgattt
     3721 atctgagaag ctggatctca aataccagaa tgatttcgcc aaattagcag aatatagtct
     3781 gggcaatact ggacttctct atacgccaaa ccaattcctg tatgaccaaa cctctatcat
     3841 caagcaagtc ttacctgact tacaaaaggt tgactatcat tcagaagcca tcagaaagac
     3901 gctgggtatt tctccaaacg tcaagcaaac tgagctctat ctagaagacc agttcgccaa
     3961 aacaaaacaa caactggaag acagtttgaa aaaactcttg tcagcggatg ctggactggc
     4021 tagtgctaac cccgtcactg aaggttatct tgtagataaa atcaaacgca acaaggaagc
     4081 cttgctactt ggcttgacct atctggaacg gtggtataac tttagctatg gtcaggtgaa
     4141 tgtcaaagac ctagttctgt accatttgga cttctttggt aaggggaatg cttcaccatt
     4201 agatactctg attgagttgg gtaaatctgg ctttaacaat cttctagcta agaataatgt
     4261 cgatacttat ggtatcagtc ttgccagtca acatggaacg acagatttgt ttagcacgct
     4321 ggaacattac cgaaaagtct ttttaccaaa tacaagcaat aatgactggt ttaaatcaga
     4381 gactaaggct tacattgtcg aagaaaaatc cactatcgaa gaggtgaaaa cgaagcaagg
     4441 gttagctggc accaagtatt ctatcggtgt ttatgatcgt atcacgagtg ccacatggaa
     4501 ataccgcaat atggtcttgc ctctcctgac cttgccagag agatccgtat ttgtcatctc
     4561 gaccatgtct agtctaggat ttggagctta tgatcgctac cgcagtagtg accataaagc
     4621 gggcaaggct ctcaatgatt ttgttgaaga aaatgcgcgt gaaacagcca aacgtcagcg
     4681 agatcactac gattattggt atcgtatttt agacgacaat gcacgtgaaa aactttatag
     4741 aaatattttg ctttacgatg cttataaatt tggcgatgat aataccgtag ggaaagctac
     4801 agaagtggca gattttgata atccaaatcc tgcaatgcaa catttctttg gacctgttgg
     4861 aaataaagtt gggcataatc aacacggtgc ttatgctaca ggtgatgcag tttattatat
     4921 gggttatcga atgttggata aggatggagc tattacttat acgcatgaga tgacacatga
     4981 ctcagatcag gacatttatc ttggaggata tggtcgaaga agtggcttgg gaccagagtt
     5041 ctttgctaaa ggattattac aagcaccaga ccatccagat gatgcgacca ttaccatcaa
     5101 ctccatcttg aaacattcaa aatctgatag tacagaaagt cgacgattac aagtacttga
     5161 tccaactaca agatttaata atgcagatga tttgaggcaa tatgtccaca acatgtttga
     5221 cgttgtttat atgttggaat atctcgaagg aaattcaatt cttaaattgg atacgaatca
     5281 aaaacaacaa cttcttagaa aagttacaaa tgagtaccat cctgatcctg atggaaataa
     5341 ggtctatgca acaaatgttg tcagaaatct aacagtagaa gaagttgaaa gactacgttc
     5401 attcaatgat ttgattgata ataatattct ttcgtctagg gaatatgcct caggtaaata
     5461 cgaaagaaat ggctacttca ctattaagtt atttgcaccg atttatgctg cattaagtaa
     5521 tgatatagga acaccaggtg acctgatggg acgtcgtata gcctatgaac tactagctgc
     5581 taaaggcttt aaagatggta tggtaccata tatctcaaac caatacgaag aagaagccaa
     5641 acaaaagggc aagacaatca atctctacgg taaaacaaga ggtttggtta cagatgactt
     5701 ggttttggaa aaggtattta ataaccaata tcatacttgg agtgagttta agaaagctat
     5761 gtatcaagaa cgacaagatc agtttgatag attgaacaaa gttactttta atgatacaac
     5821 acagccttgg caaacatttg ccaagaaaac tacaagcagt gtagatgaat tacagaaatt
     5881 aatggacgtt gctgttcgta aggatgcaga acacaattac taccattgga ataactacaa
     5941 tccagacata gatagtgaag tccacaagct caagagagca atctttaaag cctatcttga
     6001 ccaaacaaat gattttagaa gttcaatttt tgagaataaa aaatagtgtc tactattagg
     6061 aaataaagtt taaaaaggtg atgaagaaca aaccaagatt caagcaggaa ttcctactga
     6121 taatgaagta agttatgatc ttatttatca gcaggaaact cttcctgcaa caggttcatc
     6181 aacttctgag cttacagctt taggcctatt agctgttggt agtttagttc ttttggttca
     6241 taatatgacg ggaacagttt tttgctccct ctgaaaagtc atcatttgat ggcttttttc
     6301 tatatagggt aaaagatagg gtaaaagata gggtaaaagg ctatcatcgg acaaaataaa
     6361 gaaggcatga tataatataa agtagatttc tatgtcataa aacaagaact gtttggacat
     6421 cattcatttg aaaactctct atgttcaaac aatagtaaaa taaaataggg gatctaaatc
     6481 cttgctatga aaggaaaaaa ctcaatggct actattcaat ggtttcctgg tcacatgtct
     6541 aaagctcgtc gacaggtgca ggagaattta aaatttgttg attttgtgac gattttagta
     6601 gatgcacgct tgcctctatc tagtcaaaat cctatgttga ccaagattgt tggtgataaa
     6661 ccaaaactct tgattttaaa caaggccgac ttggctgatc cagcaatgac caaggaatgg
     6721 cgtcagtatt ttgaatcaca aggaatccag acgctagcta tcaactccaa agagcaagtg
     6781 actgtaaaag ttgtaacaga tgcggccaag aagctcatgg ctgataagat tgctcgccag
     6841 aaagaacgtg ggattcagat tgaaaccttg cgtaccatga ttattgggat tccaaacgct
     6901 ggtaaatcaa ctctgatgaa ccgtttggct ggtaaaaaga ttgctgttgt tggaaacaag
     6961 ccaggggtca caaaaggtca acaatggctt aaaaccaata aagatctgga aatcttggat
     7021 acaccgggga ttctctggcc taagtttgag gatgaaactg ttgcacttaa gttggcattg
     7081 actggagcta tcaaggatca gttgcttcct atggatgagg ttaccatttt tggtatcaat
     7141 tatttcaaag aacattatcc agaaaagctg gctgaacgct tcaaacaaat gaaaattgaa
     7201 gaagaagcgc ctgtgattat tatggatatg acccgcgccc tcggtttccg tgatgactat
     7261 gaccgttttt acagtctctt cgtgaaggaa gtccgtgatg gcaaactcgg taactatacc
     7321 ttagatacat tggaagacct cgatggcaac gattaaagaa atcaaagaac tccttgtgac
     7381 agtcaaggag ttagaaagcc ctattttttt agagcttgaa aaggataatc gctcaggagt
     7441 tcaaaaggaa atcagcaagc gtaaaagagc cattcaagct gaattagatg aaaatttgcg
     7501 cttggaatcc atgctttctt atgaaaaaga actttataag caaggattga ccttaattgc
     7561 aggtattgat gaggttggtc gtggtcctct tgctggtcct gtagtcgctg cggccgttat
     7621 tttacctaaa aattgtaaga ttaaaggtct caacgacagc aagaaaattc ctaaaaagaa
     7681 acatctggag attttccaag ccgttcaaga ccaagccttg tcgattggaa ttggtatcat
     7741 agataatcag gtcatcgacc aagtcaacat ctatgaagca accaaactag ccatgcaaga
     7801 agcaatctcc cagctcagcc ctcaaccaga gcaccttttg attgatgcca tgaaactgga
     7861 cttgcccatt tcacaaacct ccattatcaa aggagatgcc aactccctct ctatcgcagc
     7921 agcatctata gtagccaagg taacacgtga tgaattgatg aaagaatacg atcagcagtt
     7981 ctctggctat gatttcgcta ctaatgcagg atatggcaca gctaaacatc tggaaggcct
     8041 cacaaaacta ggagttaccc caattcaccg aaccagcttt gaacccgtta aatcactggt
     8101 tttaggtaaa aaagaaagtt aattgaaagg aaataccatg gaggaacagt cggaaatagt
     8161 ccgttctaag aaagaattcg cctttgcatc cagcactata ctatcccaag ttggtcgagg
     8221 aatcattgtc ggcctcatcg ttggaattat cgtcggatcc tttcgtttct taattgaaaa
     8281 gggcttccac ctgatacaag gagtttatca agatcaaggg tacttagtgc gcaatctttt
     8341 tgtactggtt ttgttttata tactcatctg ttggctcagt gccaaactaa cacggtcaga
     8401 aaaagatatt aaaggctcag gaattcctca agtcgaagcc gaactgaaag gcctcatgtc
     8461 cctcaactgg tggggcattc tttggaaaaa atatgtgcta ggtattcttg ctattgccag
     8521 tggactcatg ctgggtcgag agggacccag cattcaactt ggagcagttg gtggtaaagg
     8581 aattgccaag tggctcaaat ccagtccagt agaggaacgt tccttgattg ccagtggagc
     8641 tgcagcaggt ttagccgcag cctttaatgc tcctattgca gcacttctct ttgttgtaga
     8701 agaagtctat caccattttt cgcgcttttt ctgggtctca actctagcag ccagcatcgt
     8761 agcaaacttt gtgtctctac tcatgttcgg tttgacacca gtattggata tgccagataa
     8821 cattcctccc atgaccctag accagtattg gatatatctc gtcatgggaa ttttccttgg
     8881 attttcaggt tttctctatg agaaagctgt attaaacgtt ggaagagttt atgacttgat
     8941 tggtcaaaaa atccatttgg atagggctta ttatcccatc ttggctttta tccttatcat
     9001 accagtcgga atcttcttac ctcaaatcat tggtggcgga aatcagcttg tcctttcttt
     9061 aactgaacaa aattttagtt tccaagtttt attagcttac tttttaatcc gctttatttg
     9121 gagtatgatt agctatggaa gtggactgcc aggaggaatt ttcctcccca ttttagctct
     9181 tggttctttg cttggtgcct tagttggtgt tatctgtgtc aatcttggac ttgtcagtca
     9241 agagcaattc cctatatttg tcattctagg aatgagtggc tattttggag ccatatcaaa
     9301 agctccctta accgctatga tcctcgtaac tgagatggta ggagatattc gcaaccttat
     9361 gccacttggt cttgtcactc ttgtttctta tattatcatg gatttgctca aaggtacgcc
     9421 agtctatgaa gccatgctgg aaaaaatgct tccagaagaa gtatctagcg aaggagaagt
     9481 tacacttatc gaaataccag tttctgataa aattgctggg aaacaagttc atgaactcaa
     9541 cttaccacac aacgtcctca tcacaactca agtccataat ggcaagagcc aaacagttaa
     9601 cggctcaacc agaatgtatc tgggtgatat gattcacctg gttattccaa aaagtgaaat
     9661 tggaaaagtc aaagatttgt tgttgtagta tgatcattta cataatttat gttatgtaaa
     9721 tgatcagttt gatttattta gaaaaccgat tct
//
LOCUS       DBERDK010000071         9590 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.071, whole genome shotgun sequence.
ACCESSION   DBERDK010000071 DBERDK010000000
VERSION     DBERDK010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 9590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9590
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.071"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..600
                     /locus_tag="ACL73L_001716"
     CDS             <1..600
                     /locus_tag="ACL73L_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HGS0772650.1"
                     /translation="EKNTNTNVEKKDATVVAHEIKGELTYEDKVIQKIIGLSLENVSG
                     LLGIDGGFFSNLKEKIVNSDDVTSGVNVEVGKTQVAVDLNVIVEYQKNVPALYSEIRE
                     IVSSEVAKMTDLEIVEINVNVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQFEK
                     AKSGLGSGFSTVQEKVSEGVEAVKGAANGVVSHENTRVN"
     gene            631..834
                     /locus_tag="ACL73L_001717"
     CDS             631..834
                     /locus_tag="ACL73L_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HGS0772651.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKAIGDEKMEKEGAAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLSGDDK"
     gene            complement(929..1168)
                     /locus_tag="ACL73L_001718"
     CDS             complement(929..1168)
                     /locus_tag="ACL73L_001718"
                     /inference="COORDINATES: protein motif:HMM:NF025126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGS0772652.1"
                     /translation="MNERYQCLKTKEYQALLSSKGRQIFAKRKIDMKSVFGQIKVCLG
                     YKRCHLRGKRQVRIDMGFILMANNLLKYNKRKRQN"
     gene            1447..1692
                     /locus_tag="ACL73L_001719"
     CDS             1447..1692
                     /locus_tag="ACL73L_001719"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HGS0772653.1"
                     /translation="MAESIQGWLAQFLVNLFKSITFDCGKEFSKWKDISNHHDSESFF
                     ANLGCPRQRCLNEHSNRLLRCHDLPKQTDFNEVSQEF"
     gene            complement(1970..2740)
                     /locus_tag="ACL73L_001720"
     CDS             complement(1970..2740)
                     /locus_tag="ACL73L_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HGS0772654.1"
                     /translation="MVEIIYFLIIIIASGLGSISGMGGGIIIKPLMDSFGYHSVSDIA
                     FYSSFSVFIMAIISTIKRFSQSKEIKWRLIFTVSFSSVLGGFLGHLIFQVLLSQLSVR
                     LVSIVQMILLFVMLLVSFVLTDFKKTYQFDKIGFYMICGLLLGLISSFLGIGGGPLNV
                     SLLMVFFSISIKEATMYSLAIIFFSQLSHLATIVVVTGLNQYHLAPVPVIFLASICGG
                     VLGTVVSKVLPENWVRYCFKGMLFFVMGMTLYNLFHIL"
     gene            complement(2755..3603)
                     /locus_tag="ACL73L_001721"
     CDS             complement(2755..3603)
                     /locus_tag="ACL73L_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HGS0772655.1"
                     /translation="MKMKKIKGGTFMMGTNSEEGFLDDFEGPQVAVSVKDFSIADTPV
                     TNQEFAQFVKETGYKTLAERQEWSFVFILFVPEAEREGYPHPAGAPWWLQVSNACWKH
                     PYGENSNLVGLEDHPVVHVALEDALAFCNWSGMSLPTEAQWEYAARGGRQSEYPWGDT
                     LLEGGYYHANTWQGRFPYENTALDGFIGTAPVYEFLPNDFGLYQMIGNVWEWCRNPRY
                     TLLASFNEDDYELPKYGIQDEEYAIRGGSFLCHCSYCNRYRVAARNGCISTSTSSHLG
                     FRCLKE"
     gene            complement(3600..5075)
                     /locus_tag="ACL73L_001722"
     CDS             complement(3600..5075)
                     /locus_tag="ACL73L_001722"
                     /EC_number="3.1.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HGS0772656.1"
                     /translation="MVQTKQPNIILIVVDQMRADALSLNSKDKLVSTPTLDMMASVGY
                     NFENAYSPVPSCVPARAALLTGLDQDKSGRVGYQDEVPWNFTNTLPKVFKDMGYQTEC
                     IGKMHVFPSRQRLGFDHVLLHDGYLHVDRKYDKAYGSQFDYASDYLAFLKGKVGYDVD
                     LIDDGMDCNSWEARPWDKDEKLHPTNWVVSESISFLQRRDPTVPFFLKMSFEKPHAPL
                     NPPKYYFDMYMERLPQFLDLHIGNWEVLEKQIPSIYALRGKLKEDDQRRMVAAYFGLI
                     THIDHQISRFLTALKEFRHDKDTIIWFVSDHGDQLGEHYLFRKGYPYQGSIHIPSFIY
                     DPAGLIAGNRGTIKQLVKIQDIFPSLVDLAGGTTTDELDGRSVKNLLFGQYEGWRTEF
                     HGEHALGKDSSQYILTDQWKFIWFPVLNHYQLFDMKKDPHEMNDLYPSEKYQPIVRQM
                     KKKLVDFLRYREEGFVVDEELVPVELSKITPTLTKTGDSQS"
     gene            complement(5399..6754)
                     /locus_tag="ACL73L_001723"
     CDS             complement(5399..6754)
                     /locus_tag="ACL73L_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HGS0772657.1"
                     /translation="MNTMLDKMQEKLSPIAMKVGNQKFLVALRDSFVGTMPVIMTGSI
                     ALLLNAFLVDLPQQFHLESITKTFQWLVDINNLVFKGSIPIVSLLFIYCLGVNIAKIY
                     KVDTVSAGLVSLASFVISIGSTVTKSFPLANVGDVKLDQILQGIDNLAFDGKNLMVTI
                     GNVIPGNHINARGYFTAMMIGFLASIIFCKVMKKNWVIKLPDSVPPAIAKPFTSIIPG
                     FMAMYIVAILTYVFHLLSNDLLIDWVYKVLQTPLLGLSQSFFAVILMIFLNKLFWFFG
                     LHGGNVLAPIMEGLFGVAMLANLDAFQKGEPIPYIWTSGSFGAFVWFGGLGLVLAILI
                     FSRNSHYRKVAKLGLAPVLFNIGEPVNYGLPVVLNPLLFIPFVLSPVFMATVAYWATS
                     WGLVSPVTQNVTWVMPPILYGCFSTAFDWRAIILSVVCLIISVLTYFPFVKMADKTEL
                     S"
     gene            complement(6764..7081)
                     /locus_tag="ACL73L_001724"
     CDS             complement(6764..7081)
                     /locus_tag="ACL73L_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HGS0772658.1"
                     /translation="MNESNLESAMGLIMYGGEAKSNAMEAIQAAKKGDFSKANRRLAD
                     ANAALLQAHKAQTEMLTREAQGEETSISLLMVHAQDHLMTSLTFVDLAKEVVEVYERF
                     EKN"
     gene            complement(7098..7415)
                     /locus_tag="ACL73L_001725"
     CDS             complement(7098..7415)
                     /locus_tag="ACL73L_001725"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGS0772659.1"
                     /translation="MAKVTIMLACAAGMSTSLLVTKMQKAAEDKGLDAEIFAVPAPEA
                     EEIVATKEVNVLLLGPQVRYLLGDFQEKLKDRQIPVAVIPMTDYGMMNGSKVLDLAES
                     LLD"
     gene            complement(7430..8326)
                     /locus_tag="ACL73L_001726"
     CDS             complement(7430..8326)
                     /locus_tag="ACL73L_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HGS0772660.1"
                     /translation="MKRLISANPSEILQMNAEELKQSILASEGRVVLSENVVTRETFV
                     GDITNSEIARAFGADMILLNCVDVFEPKIYALDSSGDDVIHRLHQLVACPIGVNLEPI
                     DPSAKMLEETQEIVAGRVASVETLKRIEELGFDFVCLTGNPGTGVSNQEIIKAVQSAK
                     ENFSGLIIAGKMHGAGVNEPVAELSVAEQLLEAGADVILVPAVGTVPAFHDQELREVV
                     DLVHSKGGLVLSAIGTSQETSDTDTIKEIALRNKICGVDIQHIGDAGYGGLATVDNIY
                     ALSKAIRGVRHTVSRLARSVNR"
     gene            complement(8329..>9590)
                     /locus_tag="ACL73L_001727"
     CDS             complement(8329..>9590)
                     /locus_tag="ACL73L_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HGS0772661.1"
                     /translation="EKLLQEKLLPVAARLGNNKALVSIRDGITLTIPLLLIGSLLMVI
                     ASFPIPGWEKYLGDIGVADYLWKGVDSSFGLLGLVASFGIAYFMARQYKVDGIPAGIV
                     SLSSFITVTPFITGEAGAGMPTAFMASKGLFVAMILGLINGYIYQWFINHNIQIKMPD
                     GVPPAVSKSFSAIIPGAVTIVGWLIVYATLDKLSLPNLHEIAQVALGGPLGLLGNNVI
                     GLLILIFLNSSFWFVGLHGGNVVNAVMKPLWLANLDANKVAYQTGETLPNIFTSVFID
                     NFVFIGGGGATIGLVLALGYLAHKKKASKQLKTLAPITVIPGLFNINEPAMFGVPIVL
                     NILLLVPFILAPMFNLLVAWGAMASGLVPLTYTDPGWTMPPVISGLLATGSISGSLLQ
                     IVLIVLDVLLYLPFVIAIEKRFKLLED"
BASE COUNT         3309 a         2020 c         1444 g         2817 t
ORIGIN      
        1 gaaaaaaaca caaacactaa cgtagaaaag aaagatgcta ctgttgtagc tcacgaaatc
       61 aaaggggaac ttacttacga agataaagtt atccaaaaaa tcattggtct ttcactagaa
      121 aacgtttcag gtcttttggg aatcgatggt ggtttcttct caaatcttaa agaaaaaatc
      181 gttaacagcg atgacgtaac aagtggtgtt aacgtagaag ttggtaaaac acaagttgca
      241 gttgacttaa acgttattgt tgagtaccaa aaaaatgttc cagctttata ttcagaaatc
      301 agagaaatcg tatcttcaga agttgctaaa atgactgact tggaaattgt tgaaatcaac
      361 gtaaacgttg tcgacatcaa aactaaagaa cagcatgaag cagactcagt aagccttcaa
      421 gatcgcgtat ctgacgttgc tgaatcaaca ggagaattca cttcagaaca attcgaaaaa
      481 gctaaatctg gtcttggatc tggtttctca actgttcaag aaaaagttag cgaaggtgta
      541 gaagctgtta aaggtgcagc aaatggtgta gtatctcacg aaaacactcg tgtaaactaa
      601 gataaaataa atataacagg agaaattatc atgtcagtag aagaaaaatt aaatcaagct
      661 aaaggttcta ttaaagaagg tgttgggaaa gccatcggtg atgaaaaaat ggaaaaagaa
      721 ggagcggctg aaaaagttgt ttctaaagta aaagaagtcg ccgaagatgc caaagacgct
      781 gtagaaggtg ctgtagaagg tgttaaaaac atgttgagtg gcgacgataa ataaggttaa
      841 aagttacttt atctttttgg taacattagt caagatgatt ctcagaaaac aaaaagctag
      901 agattcccaa ttgcggaact ctagcttttt aattttgcct ctttctctta ttatatttca
      961 gcaggttgtt ggccatgagt atgaatccca tgtcaattct cacttgacgc ttccctctca
     1021 gatgacatct cttataaccc aaacaaacct ttatctgccc aaagacagat ttcatatcaa
     1081 tcttacgttt agcgaaaatt tgtctaccct tggaagataa aagtgcctga tattctttag
     1141 tttttaaaca ctggtaacgt tcattcatat acagtctctt ttgaggggct gattcaggtt
     1201 cataatcgca gtcaacattg atttcaaggc tgtttgcttt ctatctcccc tttttcatga
     1261 tatattttcg tacaggtgct tcaatagttt ctatagtaaa atgaaccaaa aatagtacac
     1321 gttgcggtat aatgttcttg tggcatattc attagatttt cgtaaacagt tctctcctat
     1381 ttgtgagcga acaggtatag tcaattgatt gtcagtttga agaccaagag tcgtaaggca
     1441 aaggatatgg cagaatccat ccaagggtgg ctggcacaat ttctagtgaa cctctttaaa
     1501 tctattacgt ttgactgtgg aaaagagttt tcaaaatgga aagatatttc caatcatcac
     1561 gatagtgaga gcttttttgc gaatctagga tgtccaagac agcggtgtct caacgaacat
     1621 tcaaatagac ttttaaggtg tcatgacttg ccaaagcaga cagatttcaa cgaagtcagt
     1681 caagaatttt aggctgggta aaaactcaat ttcaggagaa aatgaaataa atattcccac
     1741 aataaaacgc atagtatcaa agtttttcca acacctgata ttatacattt tttaatttta
     1801 aagacttttt cccagcctca agaaatcaca tcccacctaa atcctttgga taccaaatac
     1861 ctcatcaggt attttctgag ttggtaagac tgtctagctt aatttgacaa atggagggcg
     1921 ttaaatccag tttttacagg aagtctattt aaatattttc acactgacct tacaaaatat
     1981 gaaataaatt atataaagtc attcccatga caaagaataa catcccttta aaacagtatc
     2041 ttacccaatt ttcaggtaag acttttgata caaccgtccc caatacccct ccgcatatag
     2101 aagcaagaaa aataactgga acaggtgcta aatgatactg atttaaacct gtaaccacaa
     2161 caattgtagc caaatgagac aactgggaaa agaaaataat tgcaagagaa tacatcgttg
     2221 cttcttttat cgaaatagaa aaaaacacca tcaataaaga tacattgagc ggaccaccac
     2281 caatccctaa aaaactagaa atcaagccca atagaagacc acaaatcata tagaatccta
     2341 tcttatcaaa ttgataagtt tttttaaaat ctgttaaaac aaatgataca agtaacatca
     2401 caaatagtaa aatcatttga acaatagata ctagccgtac tgataattga gataacaaaa
     2461 cttgaaaaat aagatgacct agaaaccctc ctaaaacaga agaaaaggat accgtaaaaa
     2521 tcaatctcca ttttatctct ttactttgtg aaaatctttt gattgttgaa ataatcgcca
     2581 ttataaaaac agagaaacta gaataaaaag caatatcact gaccgaatgg tatccaaaac
     2641 tatccataag tggcttaatt ataattccac cacccatgcc tgatatagaa cccaatccac
     2701 tagctattat aatgattaga aaataaataa tctcaaccac tttttaagtc catcctattc
     2761 tttcaaacat ctaaaaccta gatggctact agtggaagta gaaatacatc catttctagc
     2821 agctactcgg tatcgattac aataagaaca atgacataaa aaggaacctc ctctgatagc
     2881 atactcctca tcttgtatcc catattttgg taactcatag tcatcttcat taaaactagc
     2941 taataaggta tatctcggat tgcgacacca ttcccaaaca ttcccaatca tttgatatag
     3001 accaaaatca ttaggtaaaa attcatagac aggagccgtt cctataaatc catctaaggc
     3061 agtattttca taaggaaatc ttccttgcca agtattggca tgataatatc caccttctaa
     3121 aagagtatct ccccaaggat attcagattg tcttcctcct ctagcagcat attcccactg
     3181 tgcttcagtc ggtaaagaca ttccgctcca attacagaaa gccaatgcat cttctaaagc
     3241 aacatgaact actggatggt cttctaaacc cactagatta ctgttctcac cgtagggatg
     3301 tttccagcaa gcgttcgaaa cttgtaacca ccacggagcc ccagcaggat gaggatatcc
     3361 ctccctttct gcctccggaa cgaacaatat aaaaacaaaa gaccactctt gtcgctcagc
     3421 tagtgtttta tagcctgttt ctttcacaaa ttgagcaaat tcttgattcg taactggagt
     3481 atccgcaatg gaaaaatcct ttacactaac agcgacttga ggaccttcaa aatcatctaa
     3541 aaagccctct tcagaatttg ttcccatcat aaatgtccca cccttgattt ttttcatttt
     3601 catgattgac tatcccccgt tttcgttaga gttggcgtta ttttagaaag ttcaacagga
     3661 actaactctt catccacaac aaaaccttct tctctatatc taagaaagtc tactaatttt
     3721 ttcttcatct gacgaacaat aggttggtat ttttcacttg gatacaaatc attcatttca
     3781 tgcggatctt ttttcatatc aaataactga taatgattta aaacaggaaa ccagataaat
     3841 ttccactggt ctgtcaagat atattgacta gaatcttttc ctaaagcatg ttcaccatga
     3901 aattctgttc gccacccctc gtactgtcca aataataaat tcttaacact ccgtccatct
     3961 agttcatctg tagtagttcc tcctgccaaa tccacgagtg aaggaaaaat atcttgaatt
     4021 ttcactaatt gcttgattgt tcccctatta ccagctatca atccagccgg atcgtaaata
     4081 aacgaaggaa tgtggatact tccctgataa ggatatccct ttctaaacag ataatgttca
     4141 ccaagctgat ctccatgatc cgaaacaaac cagataatcg tatctttatc atgacgaaat
     4201 tcttttaggg cagtcaaaaa gcgactaatt tgatggtcta tatgagtaat caatccaaaa
     4261 taagcagcaa ccattcttct ttgatcatct tcctttaatt tccctcttaa agcataaata
     4321 gacggaattt gcttttctaa tacttcccaa ttcccgatat gcaaatcgag aaattgaggc
     4381 aagcgttcca tatacatatc aaaatagtat tttgggggat ttaatggagc atgtggtttt
     4441 tcaaaagaca ttttgagaaa aaaaggaacc gttggatctc ttctttgtaa aaatgaaatg
     4501 gactcactca caacccagtt tgttggatgt aacttctcat ctttatccca aggtctagct
     4561 tcccaagaat tacaatccat tccgtcatca atcaaatcaa catcataacc aactttaccc
     4621 tttaaaaagg ctagatagtc actagcataa tcaaattgac ttccataagc cttatcatac
     4681 tttctatcaa catgtagata cccatcgtga agtaagacat gatcaaaccc aagtctctgt
     4741 ctggaaggaa aaacatgcat cttaccaata cactctgttt gataacccat atctttaaag
     4801 acttttggaa gagtattagt aaaattccaa ggcacctcat cttgataacc aactcgtcca
     4861 cttttatctt ggtctaagcc agtcaataaa gctgccctag caggaacgca agaaggaact
     4921 ggagaatagg cattttcaaa attataacct acactagcca tcatatctaa tgttggagtg
     4981 ctaactagct tatccttact attcaaggat aaggcgtctg ctctcatttg atctacaaca
     5041 atcaaaataa tatttggctg ttttgtctga accataaaat ctcctttcta atatggcaaa
     5101 agaggcacaa gaagatatct acctttactg cacccctttc tatatcaatc tctctatata
     5161 aaacaataac attcttgtta tgttttatag aaaaatgaac taaaatatga ctaaatcgat
     5221 taggaaattc aaatcatttt ctagtactat tttagaagtt acagtgtact attccaactt
     5281 caataaacta taaacctttg tctaataaca attttagtgg agataagaaa tcctacacct
     5341 aactcaatct tacacgtaat ctatttctat tttatcacaa aaaacgcaag taagaccatt
     5401 aactcaattc agttttatct gccattttca caaatgggaa ataagtcaag acactaataa
     5461 tcaaacaaac aactgataag atgatggcac gccaatcaaa tgctgtagag aagcaaccat
     5521 ataaaattgg aggcattacc caagtaacat tttgtgtaac aggtgaaaca agaccccagc
     5581 ttgttgccca gtaagctacc gttgccatga aaaccgggct aagtacaaat ggtataaata
     5641 gcaaaggatt caagacaact ggtaaaccat aatttaccgg ctcaccaata ttaaacagaa
     5701 ctggtgctag accaagttta gcaacttttc gataatgact gtttcttgaa aaaattaaaa
     5761 tagcaagtac caatcctaat cctccaaacc agacaaacgc cccaaaagac ccacttgtcc
     5821 atatataagg aatcggttca cctttttgga aagcatccag attcgctaac atagcaactc
     5881 caaacagccc ttccatgatg ggagccaata catttcctcc atggagacca aaaaaccaga
     5941 ataacttatt taaaaagatc atcagaataa ctgcaaagaa actttgagac aaacctagta
     6001 atggcgtttg taacaccttg taaacccaat caatcaataa gtcattgcta agtaaatgga
     6061 aaacataagt caagatggct actatataca tcgccataaa tcctggaatg atagaagtga
     6121 acggcttagc aatcgcaggg ggaactgaat ctggtaactt gattacccag ttctttttca
     6181 ttactttaca gaaaataata gaggctaaaa atccaatcat catggctgta aagtagcctc
     6241 tggcattaat atggtttcct ggaatcacat tcccaatagt taccatcaga tttttaccat
     6301 caaatgctag attatcaatt ccttgtaaga tctgatctaa tttcacatct cctacatttg
     6361 ccaaagggaa actctttgta actgtacttc caatcgaaat gacaaacgaa gcaagtgata
     6421 ccaaaccagc agaaactgta tcaaccttgt aaatcttagc gatattcact cccaagcaat
     6481 agatgaacaa caaggaaaca attggtatac ttcccttgaa taccaaatta ttgatgtcaa
     6541 caagccactg aaaggttttc gtaatacttt ctaggtgaaa ttgttgtggt aaatccacta
     6601 gaaaagcatt taataacaaa gcaatggaac ctgtcataat aacaggcata gtccccacaa
     6661 atgaatcacg taaagcaact aaaaacttct gattcccaac tttcattgca attggagaaa
     6721 gtttttcttg cattttatcc aacattgtat tcatactaaa ctcctaattt ttttcaaatc
     6781 gttcgtatac ttctaccact tctttcgcca agtcaacaaa agtcagactc gtcattaaat
     6841 gatcttgcgc gtgtaccatc aagaggctaa ttgatgtttc ttccccctgt gcctctcttg
     6901 tcaacatttc tgtttgagcc ttatgcgcct gtaataaggc agcattcgca tcagctaatc
     6961 ttcgattggc tttagagaaa tccccttttt ttgctgcctg aatagcctcc atagcattac
     7021 ttttggcttc cccaccatac ataattagcc ccattgcaga ttctaaatta ctttcattca
     7081 tagcattctc ctcaatctta gtctaataaa ctttcagcta aatctaagac tttagaacca
     7141 ttcatcattc cataatctgt catcggaata accgccacag gaatctgtct atcttttagt
     7201 ttttcttgaa agtcccctag taaatagcga acttgagggc ctaaaagcaa cacatttact
     7261 tcctttgttg ctacaatttc ttctgcttca ggagctggaa ctgcaaaaat ttctgcatcc
     7321 aaccccttat cttctgctgc cttttgcatc tttgtcacta gcagacttgt actcatacct
     7381 gctgcacatg ctaacataat tgttacttta gccatagctt actcctttat cacctattta
     7441 ctgacctagc caagcgagat actgtatgtc tcactcctct aattgccttg ctcaatgcat
     7501 aaatattatc gactgttgcc agtcccccat atcctgcgtc acctatatgt tgaatatcaa
     7561 ctccacaaat tttatttcta agtgcaattt ccttgatagt atctgtatca gatgtttctt
     7621 ggctagtacc aatagcactc aatacaagac cacccttact atgaacgaga tcaacgactt
     7681 cacgcaactc ttggtcatga aaggctggaa cggttccaac tgctggaaca agtatcacat
     7741 ccgcacctgc ttccaacaat tgctctgcga cagaaagctc tgccacaggc tcattcactc
     7801 ctgccccgtg cattttacca gctataatca aacctgagaa attctcttta gcagattgaa
     7861 ctgctttgat aatctcttga ttgctaactc ctgttccagg atttccagtc aaacagacaa
     7921 agtcaaaacc taactcctct attcgcttca atgtttcaac actagcaaca cgacctgcaa
     7981 caatttcctg tgtttcctct agcatctttg cagatgggtc aatcggttcc aaatttacac
     8041 caattggaca agcaacaagc tggtgtaagc gatgaataac atcatcacct gaactatcca
     8101 aagcataaat tttaggctca aaaacatcaa cacaattcaa taaaatcata tcagctccaa
     8161 aagctctagc aatttcagaa ttagttatat ccccaacaaa tgtctcacga gtaactacat
     8221 tttcagatag aacaactcta ccttcacttg ctaaaatact ttgttttaat tcttcagcat
     8281 tcatctgtaa tatctcagat ggatttgcac taattaaacg tttcattctc aatcctccaa
     8341 taacttaaac cgtttttcaa tagctattac aaatggtaaa taaagtaaga catccaaaac
     8401 aatcaataca atttgtaata atgaaccaga aatacttcct gtagcaagta aaccacttat
     8461 aacaggtggc atagtccaac ctggatctgt ataagttagc ggaaccaaac ctgatgccat
     8521 tgctccccac gctactagta agttaaacat tggagctaag ataaatggaa ctagtaacaa
     8581 aatgttcaaa acaataggaa cgccaaacat tgctggttca tttatattaa ataatccagg
     8641 tataacagtt ataggtgcca aagtttttag ttgtttacta gctttcttct tatgtgctaa
     8701 atatcctaac gcaagaacta aacctatggt tgcaccaccg ccacctatga atacaaaatt
     8761 atcgataaat acgctagtaa aaatatttgg aagagtttct ccagtttgat aagctacttt
     8821 atttgcatct agattagcta accataacgg tttcataact gcattaacaa catttcctcc
     8881 gtgtaatcct acaaaccaaa aactactatt aaggaaaatt aagataagaa gaccaataac
     8941 attatttcct aaaagtccaa gtggacctcc caaagctact tgagcaattt catgaagatt
     9001 aggtaaactt aatttatcta aagttgcata aacaatcaac caaccaacaa tagtcactgc
     9061 accaggaata atggcactaa agcttttaga tactgctggt ggaacaccat ccggcatttt
     9121 tatctgtata ttatgattta taaaccattg gtagatatag ccattaatca atcctaaaat
     9181 catagcaaca aataaacctt ttgatgccat gaaagcggta ggcattcctg ctccagcctc
     9241 tcctgtaatg aaaggcgtaa ctgtaataaa agatgataat gacacaatac cagcaggaat
     9301 accatcaact ttgtattgtc tagccataaa gtaagcgata ccaaaactag ctacaagacc
     9361 aagtaatcca aaactactat caacaccctt ccataaataa tcagcaacac caatatcacc
     9421 cagatatttt tcccatcctg gaataggaaa acttgcaata accattagta atgaacctat
     9481 caacaataaa ggtatagtta gagtaatacc gtctcgaata gaaaccaacg ctttattatt
     9541 acccaatcta gccgctactg gaagtaactt ttcttgtaat aatttttcca
//
LOCUS       DBERDK010000072         9298 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.072, whole genome shotgun sequence.
ACCESSION   DBERDK010000072 DBERDK010000000
VERSION     DBERDK010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 9298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9298
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.072"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(27..1706)
                     /locus_tag="ACL73L_001728"
     CDS             complement(27..1706)
                     /locus_tag="ACL73L_001728"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HGS0772662.1"
                     /translation="MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAP
                     KGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLCLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRDLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
     gene            complement(1708..1941)
                     /locus_tag="ACL73L_001729"
     CDS             complement(1708..1941)
                     /locus_tag="ACL73L_001729"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002947111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase subunit epsilon"
                     /protein_id="HGS0772663.1"
                     /translation="MIYKVFYQETKERSPRRETTRALYLDIDASSELEGRITARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGAFEITEF"
     gene            2221..2436
                     /locus_tag="ACL73L_001730"
                     /pseudo
     CDS             2221..2436
                     /locus_tag="ACL73L_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282487.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2759..2911)
                     /gene="cibB"
                     /locus_tag="ACL73L_001731"
     CDS             complement(2759..2911)
                     /gene="cibB"
                     /locus_tag="ACL73L_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HGS0772664.1"
                     /translation="MMKNLNNYREISNKELQEIKGGFGVGVGIALFMAGYTIGKDLRK
                     KFGKSC"
     gene            complement(2913..3098)
                     /gene="cibA"
                     /locus_tag="ACL73L_001732"
     CDS             complement(2913..3098)
                     /gene="cibA"
                     /locus_tag="ACL73L_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
                     /protein_id="HGS0772665.1"
                     /translation="MTNFDILDNQFLSLSENELSDIDGGLAPLVIFGVAVSWKAIAGG
                     TALIGSGLAAGYFLGGD"
     gene            3276..3959
                     /gene="tsaB"
                     /locus_tag="ACL73L_001733"
     CDS             3276..3959
                     /gene="tsaB"
                     /locus_tag="ACL73L_001733"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HGS0772666.1"
                     /translation="MKVLAFDTSSKALSLAILEDKQVLAETTINIKKNHSITLMPAID
                     FLMASLDWTPKDLDRIVVAEGPGSYTGLRIAVATAKTLAYTLNIELVGMSSLLALVPH
                     QQEGLFVPLMDARRNNVYAGFYENAKPVMAEAHLSFEEVLEKVKGTSQVTFVGEVGPF
                     VEQIQKHLPRTDYKETLPNAANLALLAWDKEADSLHDFVPNYLKRVEAEENWLKNHTE
                     SGESYIKRL"
     gene            3956..4393
                     /gene="rimI"
                     /locus_tag="ACL73L_001734"
     CDS             3956..4393
                     /gene="rimI"
                     /locus_tag="ACL73L_001734"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HGS0772667.1"
                     /translation="MIEIKRIQQQPDLAKAIYAVMIAVYPVSPWTLEQIQADLFQDQT
                     WYALAYDGAEVIGFLAVQETLFEAEVLQIAVKGAYQGKGIASALFAQLPTDKEIFLEV
                     RKSNQRAQAFYKKEKMAVIADRKAYYHDPVEDAIIMKREIDEG"
     gene            4383..5393
                     /gene="tsaD"
                     /locus_tag="ACL73L_001735"
     CDS             4383..5393
                     /gene="tsaD"
                     /locus_tag="ACL73L_001735"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HGS0772668.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKAFA
                     WAHGLPLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLHHNAEQKGESLSTEDLCASFQAAVMDILMAKTKKALEKYPVKTLVVAGGVAANK
                     GLRERLAAEITDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFAGWDLNAKPSLAFDT
                     ME"
     gene            complement(5433..6739)
                     /locus_tag="ACL73L_001736"
                     /pseudo
     CDS             complement(5433..6739)
                     /locus_tag="ACL73L_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011680908.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(7075..7766)
                     /locus_tag="ACL73L_001737"
                     /pseudo
     CDS             complement(7075..7766)
                     /locus_tag="ACL73L_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001839528.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            8009..8563
                     /locus_tag="ACL73L_001738"
     CDS             8009..8563
                     /locus_tag="ACL73L_001738"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HGS0772669.1"
                     /translation="MQYSCGKININIPDGYGDIKDIVFSAHIIVRYNNGHCGGIDPHI
                     IGLCKKQIRRMSLYPILIIVSRDSKVIDDYKNLDIAYVDCTQCSNNFETALHVKNILK
                     LLKIRLIHCHGYSTNYFLYMLKKLDKNGFGKVKTVITCHGWVEYNLKKKFLTYFDFWT
                     YSMGDAFICVSETMKKRLESIIKK"
     gene            8691..9152
                     /locus_tag="ACL73L_001739"
     CDS             8691..9152
                     /locus_tag="ACL73L_001739"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HGS0772670.1"
                     /translation="MDPEKRPDRFLEFAEKLFLVREDVIFIMAGNGSMWAALKEKICH
                     LKCRDNFRLLGEIYPATIVYQISDLLYIPSDTEGIPMCVLESMSQGTPVLASNVGGLS
                     EIIEHRVDGFLFEKEDVEGVCACANFLLNDSEYLKYIGENSKSKIRKHESI"
     gene            9176..>9298
                     /locus_tag="ACL73L_001740"
     CDS             9176..>9298
                     /locus_tag="ACL73L_001740"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGS0772671.1"
                     /translation="MDRNIDQELVSIIIPTHNRYESLIRAVKSCLHQSYKNIEVI"
BASE COUNT         2724 a         1694 c         2080 g         2800 t
ORIGIN      
        1 gcgaagctga cgtggtttga agagtattat tcttcatctg gtgtgaggat catcgggatg
       61 atgatcggtt cacgttcggt attttcatag aggaaggggc gaatagcgtt gacaatggca
      121 ccattgacag attgcacgct agcatcctta tttttcagtg cgatacgaat ggcattgaag
      181 aggatacgct ggctttggcg aatcaagtcg ccagactctc tcatgtagac aaagcctcgg
      241 ctgaggatgt ctggaccaga tagaatcatc tgcgatttga agtcaacagt tgcgactgcc
      301 agaacgacac cgtcttcaga tagatcgcga cgatctttga ggacagctgc gccaatttca
      361 ccgatacgat ttccatcgac atagatatct tgggcgttga aatggcctgc gatacgagct
      421 gagtcagcag taagggcaag cacatcgcca ttgctcatga taaagatatt gtccttctca
      481 acaccagtat ccactgctag tccagcgtgg actttttgca tgcggtattc accgtggaca
      541 ggcatgaagt attttggctt aatcaagcag agcatgagtt tttgctcttg ctgaccaccg
      601 tgtccagatg tatggatatt gttcacttta ccgtggataa cttcgacacc agcttcagaa
      661 atgatgttaa tcagcttgtt gacgctagta gtgtttccag ggatgggact agaagagaag
      721 ataacggtat cacctggttg taattgtact tgacggtggg ttccgttggc gatacgagag
      781 agggctgcca taggctcacc ctgactacct gtacagagga taagaacttc tcctgcagga
      841 taatctttga tttcatttgg ctcgataaag gttcccttag gagctttgat gtagccaaga
      901 tcgattccgt tgacaatggc cttttccata gaacgaccaa agaccgcaat cttgcgtcca
      961 gtcttaacag cagcttctgt tgcctgctgg agacggaaga tatttgaggc aaaggatgca
     1021 aagatgatac gtccttcaat accttggata atcttcataa tggactgacc aacgactttt
     1081 tcagagttgg taaaggttgg tacttccgca tttgtcgagt cagacaggag acagagcacg
     1141 ccttcttcac caagcgcagc catacgatgc aagtccgcag gttctccaac tggagtaaag
     1201 tcgaacttaa agtcacccgt acagacgatt ttcccttgag gagtatgaat gacaatcccc
     1261 aaaggctctg gaatagagtg agtcgttcta aagaaagttg ccttgagatt tttaaaggtc
     1321 aactcggtgt tgtggttgat ttcgtaaagt ttggcgttgc gcaagaggcc gtgttcttcg
     1381 agtttcccac ggatcaaagc caaggcaagc ggtccagcat aaatagggac atttgcttgc
     1441 ttgagtagga acggaatccc accaatgtgg tcctcgtgtc cgtgtgtgat taaaacagcc
     1501 ttgacgcggt cgatattgtc tacgatgtaa gagtagtcag gaatgacata gtcgatacca
     1561 agcaagtcat cttctgggaa tttaatccca gcatcgacga tgataatctc gtcttggtat
     1621 tcaattccgt aagtgttttt cccgatttct cctagaccac cgatggcaaa aacgccgact
     1681 tcttcaggtt taagagtgta ggccatatta gaactccgta atttcgaagg cgccagtttc
     1741 tttttcgtaa tcgagcaatt tgtcagacaa gagttcgata tattcgatat tgtactctgg
     1801 gcgattttct tcgacaagtt ggcgagcagt gatacggccc tcaagttctg agctggcatc
     1861 gatgtctagg taaagcgcgc gtgttgtttc acggcgtggg ctacgttctt ttgtttcttg
     1921 ataaaaaact ttgtaaatca tatagttcct ttctatttac aggtcagctt attccaaact
     1981 tttagcagat atttgcttga tttcattcca ttttgtgtct agattgacct tgattcgtta
     2041 caattatttc aattatacca caaaatgaaa aattagtaaa agacggaaac gagaaagtta
     2101 gggggcaatt tctttggtaa gcgtttacaa aaataaagaa aatgtattat gttgataacg
     2161 gaagtgaaat ttattgcttg tcataattcc atatagattt taagtcaagg aggttgccct
     2221 atgtataact tatttgcttt tgctatcgga gttcgtttgt tagcttttat cgtttttgtt
     2281 gcttgcgttt atggaggaaa tcttccattt gccaaactgg aacgatgaca aaccaagttc
     2341 ttgtggaaaa ttacctttgt gactctctac tcgctttttc tcctcctcgt gccctatgtc
     2401 gtttggtttg tattttactt tggattaaat gcttagatgc cctgcttatg ctggggtttt
     2461 ttgtttgggg atgtagagat agtagattta cgacacgaaa attccacccc atctatcaag
     2521 atgaggtggt ctttgcattt ttctaacatg acatgacaat ggaattttca aagcttatta
     2581 tttttatggg agtcattgtt ctgctcaaaa atagagttga agcataaaaa taaaacaata
     2641 caaacagata aatcgaatgg taaagggata gtagtaatgt tcaaatcatt gagaatgctg
     2701 agcaaactgt aagagatgaa gacaataaaa tttatccttc taaaaatgtg tttcttatct
     2761 agcatgactt accaaacttt ttacgaaggt cttttccaat ggtataacct gccataaata
     2821 aagcgatacc aacacctaca ccaaagccac ccttgatttc ttgcaattcc ttattagaaa
     2881 tttcacgata gttgttcaaa tttttcatca tattaatctc ctcctaaaaa ataaccagct
     2941 gccaaaccag aacctataag tgctgttcca cctgcaatag ccttccaaga tactgctact
     3001 ccaaagataa ccaagggagc gagaccgcca tcaatatctg ataattcatt ttcagataag
     3061 gataaaaatt gattgtcaag aatgtcaaaa tttgtcattg aactttcctc cttttttctt
     3121 gttcactttt atattcggaa aagaaaagga aaagtgagca tttttttaaa ttatttttag
     3181 ttaagtgagg gcactcttgt ctgggggcaa agaaaattcc catgtgccag cttttgtagt
     3241 ataatagtaa gcagacaaaa agataggaat gtgttatgaa agtattagct tttgatacgt
     3301 ccagcaaggc tctttctctg gctattttag aggataagca ggttcttgcc gagacgacga
     3361 ttaatattaa gaaaaatcac agtattactc ttatgcctgc catcgatttt ttgatggcaa
     3421 gtttggattg gacacccaag gatttggacc gaatcgtggt agctgaaggg ccgggtagct
     3481 atacaggctt gcgaattgcg gtagcaactg ctaagacctt agcttacacc ctgaacatcg
     3541 agttggttgg tatgtcgagt ctcttggctc tggtgcccca ccaacaagaa ggtttgtttg
     3601 tccccttgat ggatgcgcgt cgcaataatg tttatgcagg attttatgaa aatgctaaac
     3661 ctgtcatggc agaagcgcac ctatcttttg aagaggtgct agaaaaagtc aagggtacta
     3721 gtcaggtaac ctttgtcgga gaagttggcc cctttgttga gcagattcaa aaacacttgc
     3781 caaggactga ttacaaagaa acattgccca atgcagctaa tctagctctt ttggcctggg
     3841 acaaggaagc agactccttg catgattttg tgccgaatta cctcaaacga gtcgaggctg
     3901 aggaaaactg gctcaagaac cataccgagt ctggtgagtc ctacattaaa cgcctatgat
     3961 agaaatcaaa cgaatccaac agcagcctga tctagctaaa gccatctacg ctgttatgat
     4021 agctgtttac ccagtcagtc cttggacgct ggaacaaatc caagctgacc tgttccaaga
     4081 ccagacttgg tatgctctgg cttatgatgg ggcagaagtg attggctttc tagctgttca
     4141 ggagactctc tttgaagcag aagtcctgca aatcgctgtc aaaggagcct atcagggtaa
     4201 gggcattgcg tcagccttgt ttgctcaatt gccgacagat aaggagattt tcctcgaagt
     4261 cagaaagtca aatcaacgag cgcaagcatt ttacaagaaa gaaaaaatgg cggtcatcgc
     4321 tgatcgaaag gcctactacc atgacccagt cgaggacgcc attatcatga agagagaaat
     4381 agatgaagga tagatatatt ttagcatttg agacatcctg tgatgagacc agtgtcgccg
     4441 tcttgaaaaa cgacgatgag ctcttgtcca atgtcattgc tagtcaaatt gagagtcaca
     4501 aacgttttgg tggcgtagtg cccgaagtag ccagtcgtca ccatgtcgag gtcattacag
     4561 cctgtatcga ggaggcattg gcagaagcag ggattaccga agaggatgtg acagctgttg
     4621 cggttaccta cggaccaggc ttggtcggag ccttgctagt tggtttgtca gctgccaagg
     4681 cctttgcttg ggctcacgga cttccactga ttcctgttaa tcacatggct gggcacctca
     4741 tggcagctca gagtgtggag cctttggagt ttcccttgct agccctcttg gttagcggtg
     4801 ggcacacaga gttggtctat gtttcggagg caggagatta caagattgtt ggggagacac
     4861 gagacgatgc agttggggag gcttatgaca aggtcggtcg tgtcatgggc ttgacctatc
     4921 ctgcaggtcg tgagattgac gagctggctc atcaggggca ggatatttat gatttccccc
     4981 gtgccatgat taaggaagat aatctggagt tctccttctc aggtttgaaa tctgccttta
     5041 tcaatcttca tcacaatgcc gagcaaaagg gagaaagcct gtctacagaa gatttgtgtg
     5101 cttccttcca agcagcagtt atggacattc tcatggcaaa aaccaagaag gctttggaga
     5161 aatatcctgt taaaacccta gttgtggcag gtggtgtggc agccaataaa ggtctcagag
     5221 aacgcctagc agccgaaatc acagatgtca aggttatcat cccccctctg cgactctgcg
     5281 gagacaatgc aggtatgatt gcctatgcca gcgtcagcga gtggaacaaa gaaaacttcg
     5341 caggctggga cctcaatgcc aaaccaagtc ttgcctttga taccatggaa taaagagtgg
     5401 ctctttgtca agtgtagtgg gtaggcgaaa agctacaatc tggagagtac gaaattcgta
     5461 ctctcttttt tgatgttcaa agtcatcaaa atccgttttt taaaattgtt aaagttccgg
     5521 aaaccaaagg ctttgcgctt gataacctta atcaaattat tggtcgcttc aagtttagcg
     5581 ttggaataag gcagcttaag tgcgttgata atcttatctc tatcccttaa aaaagtccaa
     5641 aagaccgttt gaaaaagcgg atgaaccgtt ggcagttctt gctcaatcaa tccaaagaaa
     5701 tggtcggcat tcttctcttg aaaatgaaag agcaggagtt gatagagttc gtagtgaacc
     5761 tgtaatccct cagagtaaga aagcagctta tctcgtactt cccgatgggt taagtgcatt
     5821 cgaaacgtgg gctgatagaa tcgtttctga cagagcttga agctatcctg gtgtagtaaa
     5881 tcagtccagt ggactgattt agcccgagcc tagaaacgaa aaagcgaggg ttccaaaagc
     5941 gcttgagcgc ccgatactcc aaggattttc ggtcaaattg gttcatgatt tggattcgta
     6001 ctcggttcat agctcggctc agatgttgga caatgtggaa gcggtcaaga acaatcttcg
     6061 cctttggaaa gagttgctta gcgagccgat aataagggct gtacatgtcc atggtgatga
     6121 cctcgaccag ctcccgaacc tctctctgat agcgttggaa atggtttcga atcaccgcat
     6181 gagttcgccc gtctaaaatt gcgaggatgg atttggactc aaaatcttgg gcaatgaagc
     6241 tcattttgct ctttttgaag ctatactcat cccaactcat aacttttggc aacttggtcc
     6301 aatcggtttc aaacttaaat tccctcagtt ttcggatgac ggtggaagtt gagaccgcca
     6361 atctgtgggc aatctcagtc atagactgct tttcaaggag gagctgagcg attttttgtc
     6421 tcaccatgtt ggaaatctgg caatttttct tgacaatgga tgtttgagaa acgaccacct
     6481 taaggcaatt cttgcactga aagcggcgct ttttgagatg tagtaccgtg ggtaaaccct
     6541 gacagtcgag aagcggaatt ttagaggctt tctggaagtc gtatttgatc atcttccctt
     6601 gacaatgagg acaaggagga gcgggggaat ccaactttgc ctgaacgact agatgggttt
     6661 ggtatttcag aacagacaag attttgatat ttttgtcttt caatccgagc aaatctgtgg
     6721 tattcttaag ttgttccata tgaacccttt ctaatgagtt ggttagacgc ttttcattat
     6781 aggtcatatg gggctttttt tctacaagaa acgaccctat aattcctggg gtgggattac
     6841 ccactacaga aattatagag ccaaaacatt ccaaagtctt gtctgactct ggaatgtttt
     6901 ctgttcacag ttgttttgta actgcctaat aagtaaccgc gctataacga ggtatctacc
     6961 gagattgtca attctccagt atactgttat aaaaaacaaa actggaggat ttttttatgt
     7021 ccccccatcg tcccatcgac tgttccgtag ctgcccaata acggagtata gtcggctgaa
     7081 acaagaatag gacaaaagtg cctcaagaaa agcatcgtaa tttgacaata ttattctgag
     7141 atgtttttga tgtaagccca tattttctca atgggattat actcgggtga ataggtagga
     7201 agtggtaaca gtctatgccc tgctccttac acatgttcat tctgtgaaac cttgcattgt
     7261 ccataatgat aagggatggt gtatctaaag taggtagtaa gaatttttgg aagcaagctt
     7321 cgaaaaagtc actcgtcatc gtatctttat aggtcatcgg agccatagta tattgaaaca
     7381 aaaaagggca ttagtacttc tataacagta tcgcaatctc tggtgtgcgt tgtctcgatc
     7441 ttaattcaaa taattatatc ttcttccaga gagactaact gcctgctcaa ggctcgatca
     7501 tattcgagat aaaaataggt ctcaacccct gtctcataaa tataaatagg agtcaggtag
     7561 cttaagtgat tcaattcttt aaggaaccga tttacttttt ctgggtcttg ttcgcagtag
     7621 gtacagctct tttttagact atatcccata gcctttagag cgtaatgaat agttgttgga
     7681 ggacagttga attcagcagc tatttcagtc aaataagcat cgggatgagt ttcaatatag
     7741 atctttaatt tatctctatc aactttttca attaatagtg gaagttttct taaagttagt
     7801 gtgttgaaac gtttttatac agtgctaaaa tttgaagtgt agtgcagatg tatttgtaat
     7861 agtattatag caattctttg gagaaggtcg agacaagaga aaataggtat ctttgagata
     7921 taagcatgat ttactataaa attattgagt tattcataaa aatttctcta tcaaatatca
     7981 taatgcgtcg tttaaggaga tggttacaat gcaatatagt tgtggaaaaa taaatattaa
     8041 tatccctgat ggatatggag acattaagga tatagtgttt tcggctcata ttatagttag
     8101 gtataataat ggacattgtg gaggtataga tccgcatata attggacttt gtaaaaagca
     8161 gatcagaagg atgtctctat atcctatttt gataatcgta tctagagatt caaaggttat
     8221 tgatgattat aaaaatttag atattgccta tgttgattgt actcaatgtt caaataattt
     8281 tgaaactgcc ttacacgtta aaaatatttt aaaattatta aaaatccgac ttatacactg
     8341 tcatggctac tcaactaatt attttttata tatgttaaaa aaacttgaca agaatggatt
     8401 tggaaaggtt aagacagtga taacatgcca tggatgggtt gaatataact taaaaaagaa
     8461 attcttgact tattttgatt tttggacata ttctatggga gatgctttta tttgtgtatc
     8521 tgaaactatg aaaaaaagat tggagagtat aataaaaaaa taagaagata gttgcaataa
     8581 ataacggaat taatgtatca aatagcgatt tagatgtagt aggtgttcaa gactttaaaa
     8641 aagaattttg tattccaaat aacaaaaaaa tcatttgtta tgttgaaagg ttggatccag
     8701 aaaaaaggcc ggatagattc cttgaatttg cagaaaaatt atttttagtc agagaagatg
     8761 tgatttttat tatggctgga aatggtagta tgtgggctgc tttgaaagaa aaaatttgcc
     8821 atttgaaatg tagagataat ttcaggttac ttggtgaaat ctatccagct actatagttt
     8881 accaaatatc ggatttattg tatattcctt ctgatacaga aggaatacct atgtgtgttt
     8941 tagagtcaat gtcacaagga actccggttt tagctagtaa tgttggcggg ttaagtgaaa
     9001 ttattgaaca tagggttgat ggatttttat ttgagaagga agatgttgag ggagtgtgtg
     9061 cttgtgctaa ttttttactc aatgattctg agtatttgaa atatataggt gagaatagta
     9121 aatcaaaaat aagaaaacat gagagtatat gatgaattat tagagaagag tagtcatgga
     9181 taggaatata gatcaagaat tagtaagtat tataatacca acacataata ggtatgaatc
     9241 tctgattaga gctgtgaaaa gttgtttaca tcaaagttat aaaaatatcg aagttata
//
LOCUS       DBERDK010000073         9077 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.073, whole genome shotgun sequence.
ACCESSION   DBERDK010000073 DBERDK010000000
VERSION     DBERDK010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 9077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9077
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.073"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..311
                     /locus_tag="ACL73L_001741"
     CDS             <1..311
                     /locus_tag="ACL73L_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772672.1"
                     /translation="QVKVVDKLTDSESKKYGEYIDLYESCRQNNLSVLLNFLQNSDDK
                     RGVIASNSVRIMFESLDKADQIAALNICRNYVARENVLNKEDAEKLLNEIDKKLEISR
                     "
     gene            367..1062
                     /locus_tag="ACL73L_001742"
     CDS             367..1062
                     /locus_tag="ACL73L_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HGS0772673.1"
                     /translation="MPVNEYGQMIGESMEGYTPGALPSIDFLEGRYARIEALSVEKHA
                     EDLLAVYGPDTPREMWTYLFQEPVADMGELVSLLNQMLARKDRFYYAIIDKATGKALG
                     TFSLMRIDQNNRVIEVGAVTFSPELRGTRIGTEAQYLLARYIFEELNYRRYEWKCDAL
                     NLPSRRVAERLGFVYEGTFRQAVVYKGRTRDTDWLSMIDMDWPQVKDRLETWLRPENF
                     DKNGQQYKSLREL"
     gene            1074..1490
                     /locus_tag="ACL73L_001743"
     CDS             1074..1490
                     /locus_tag="ACL73L_001743"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS0772674.1"
                     /translation="MITIKKQEIVKLEDVLHLYQAVGWTNYTHQPEMLEHALSHSLVI
                     YLALDGDAVVGLIRLVGDGFSSVFVQDLIVLPIYQRQGIGSALMKEALKDYKDAYQVQ
                     LVTEETEKNVGFYRSMGFEILSTYNCIGMTWANREK"
     gene            complement(2305..2397)
                     /locus_tag="ACL73L_001744"
     CDS             complement(2305..2397)
                     /locus_tag="ACL73L_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HGS0772675.1"
                     /translation="MMELVLKTIIGPIVVGVVLRIVDKWLNKDK"
     gene            2531..3529
                     /gene="ruvB"
                     /locus_tag="ACL73L_001745"
     CDS             2531..3529
                     /gene="ruvB"
                     /locus_tag="ACL73L_001745"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HGS0772676.1"
                     /translation="MSRILDNEIMGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEA
                     AKMRDEALDHVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNELE
                     PGDVLFIDEIHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTR
                     AGMLSNPLRARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPR
                     IANRLLKRVRDFAQIMGNGVIDDVITDKALTMLDVDHEGLDYVDQKILRTMIEMYGGG
                     PVGLGTLSVNIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAKAYEHLGYEYSEK"
     gene            3510..4082
                     /locus_tag="ACL73L_001746"
     CDS             3510..4082
                     /locus_tag="ACL73L_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HGS0772677.1"
                     /translation="MNTVKNKQEILEAFRENPDMMAILTIIRDLGLKDSWLAAGSVRN
                     FIWNLLSDKSPFDHETDIDVIFFDPDFSYEETLLLEKKLREDFPQYQWELKNQVYMHQ
                     HSPHTASYTSSRDAMSKYPERCTTVGLRLNEESDFELYAPYGLEDILNFQVRPTPHFL
                     ENEDRMELYQTRLSKKNWQEKWKNLIFKNT"
     gene            4313..5071
                     /locus_tag="ACL73L_001747"
     CDS             4313..5071
                     /locus_tag="ACL73L_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HGS0772678.1"
                     /translation="MFGFFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKVITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDRLPKQTFEALTKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKYLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEVALQEAILAYNRRHRRFGGV"
     gene            5080..5883
                     /locus_tag="ACL73L_001748"
     CDS             5080..5883
                     /locus_tag="ACL73L_001748"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HGS0772679.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLLQIAIGIIAMLAMHE
                     LLKMRGLETMTMEGLLTLFATFALTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKALLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIIFMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLFGLF"
     gene            5905..7164
                     /gene="rseP"
                     /locus_tag="ACL73L_001749"
     CDS             5905..7164
                     /gene="rseP"
                     /locus_tag="ACL73L_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HGS0772680.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKRINLSGK
                     KLDQTALPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PETAQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPEDSQG
                     RYLLGVQPGVKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAAKNGIENILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRRKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            7177..9030
                     /locus_tag="ACL73L_001750"
     CDS             7177..9030
                     /locus_tag="ACL73L_001750"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HGS0772681.1"
                     /translation="MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPALLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     NDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVR
                     NAVVGANEDGYHLTGVNPGRDFTAEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALTEKLEASLMGAGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK"
BASE COUNT         2619 a         1614 c         2124 g         2720 t
ORIGIN      
        1 cgcaggtaaa agttgtagat aaacttactg attctgaatc gaaaaaatac ggtgaatata
       61 ttgatttgta cgaatcgtgt cgtcagaaca atttaagtgt tctacttaac tttcttcaaa
      121 attcggatga taaacgagga gttattgcta gtaactcagt gagaattatg tttgaatctt
      181 tggataaagc agaccaaatt gcggccctga atatttgtag aaactatgta gcacgagaaa
      241 atgttttaaa taaagaagat gcagaaaaat tgttaaatga aattgataaa aaacttgaaa
      301 ttagcagata ggacaattca aatagagttg agtaaaaagt ttgaaaagtg aaacaaggag
      361 aaagctatgc cagtaaatga atatggtcag atgattggtg agtcaatgga aggttataca
      421 cccggtgcac tgccttctat tgatttctta gaagggcgtt atgctaggat agaggctctc
      481 tcggtagaaa aacatgcgga ggatttgcta gctgtttatg gcccggatac tcctcgggag
      541 atgtggacct atctttttca ggagccagta gcagatatgg gggagctggt tagcctttta
      601 aatcagatgt tggctcgtaa ggaccgtttt tactatgcaa tcatagacaa ggcgactggt
      661 aaggctttgg gaactttttc tctcatgcgc attgaccaga ataaccgagt aatagaagtc
      721 ggagcagtca ctttttctcc agagctaagg ggtacacgga taggaactga ggctcagtat
      781 ctcctagctc gctatatttt tgaggagcta aactatcgtc gctacgagtg gaaatgcgat
      841 gccttaaatc tgccatccag acgagttgcg gagcgtttgg gatttgtcta tgaaggaacc
      901 ttccgccagg cggtggttta taaggggcgt acgagagata cggattggtt gtctatgatt
      961 gatatggact ggcctcaagt caaagatcgt ttggaaacat ggctgcgtcc tgaaaatttt
     1021 gataaaaatg gacagcagta caagagcttg agagaacttt aagaggtgtt gagatgatta
     1081 ctattaaaaa gcaagaaatt gtcaagctag aggatgtttt gcatctctat caggctgtcg
     1141 gttggacaaa ttatacccat caaccagaga tgctggagca tgccttatcg cattcattag
     1201 taatttatct ggcacttgat ggtgatgctg tggtgggctt gattcgtttg gttggagatg
     1261 gattctcatc agtttttgta caggatttga ttgttttacc aatctatcag cgtcaaggga
     1321 ttggtagtgc cctaatgaaa gaggccttaa aagattacaa agatgcttat caagtccagc
     1381 tagtgacaga agagacagaa aaaaacgtgg gattttatcg ttctatgggc tttgaaatct
     1441 tatccaccta taattgtata gggatgactt gggcgaatcg agaaaaataa caaaacttgt
     1501 tttttcttaa gcaaaattta aggatggtct agtattatat aatcattaaa taaagacctc
     1561 ctaactttat ttaataaaat cctaaacttt tttcatcaca atctcctaat gaagccaccc
     1621 aatcaggtgg cttttttgcg tggtgaggtg ctggtgatag aaattttttg caaaatagta
     1681 aaattttaga aaagttaagg tagttttaag tttcgtcttg tatcatgtag ttattaaata
     1741 aagacctcct aactttattt aataaaatcc taaacttttc tttttcataa taatctccct
     1801 taactccacc caatcaggtg gagtttttta gctctatttc aggcttttgg ggactattct
     1861 aaaaataatt tttcgatatt tttcggtatt tttcggattt tggtcgggga attggcgggg
     1921 acttttttag cgaatataac taagaaatag gtctgtggtc gcttcagcta gttcaaactc
     1981 aatttgattg taagtgactg ttaattgaag aactggaact actgtaataa tgattttgct
     2041 tgtctagtag tggcgtatta aatgagaagt gacatgtaag attgtctctt cgtttacgaa
     2101 atcaaaattt ctatgaaatt gatagaatcc ctaacgttct cttaatctta accgtcttgt
     2161 cgtacagcgg ttgaagaaaa tgtaaacctt tgacaaaatt cgtgaactat gggataataa
     2221 aaaggaattg agtactagtt ctatatcata ggctagaaaa gaccccaagc tcacgaccaa
     2281 ataagcttga ggtctttttt gacactattt gtccttgttt agccatttat cgactatacg
     2341 aagaacgaca ccgaccacaa ttggtccgat gatagttttg agtactaatt ccatcatggg
     2401 ctatctcacc tcctttcgta ggcggtgtag cagtgtcgta gaatattata ccacataagt
     2461 gctaaactta ggagtcaccc aatcaggtgg cttttttgtt tgtggcttgg ttttttgata
     2521 taatagagcc atgagtagaa ttttagataa tgagataatg ggggatgagg agttagtaga
     2581 acgcacgctc cgtcctcagt atttacgtga atatatcgga caggataagg tcaaggacca
     2641 gctacaaatc tttattgaag ctgccaaaat gcgggatgaa gcgctggatc atgtgctctt
     2701 atttgggcct ccaggtctcg ggaaaacgac catggccttt gttattgcca acgaactggg
     2761 agtcaatctt aagcagacgt cgggtccagt cattgaaaaa gccggagatc tggtagctat
     2821 tttgaatgag ttagagcctg gggatgtact ttttattgat gagatccatc gtttgccaat
     2881 gtcagtggaa gaggtgcttt atagtgctat ggaggacttc tacatcgata ttatgattgg
     2941 ggctggtgag ggtagtcgta gtgttcattt ggagttacca ccttttacct tgattggtgc
     3001 gacgactcgg gctggtatgc tctccaatcc gctacgggca cgttttggga ttacaggtca
     3061 tatggagtat tatgctcatg ctgatttgac agaaattgtc gagcggacgg cagatatttt
     3121 tgagatggaa atcacccatg aggcagcatc tgagttggcc ctacgtagtc gtgggacccc
     3181 tcgtattgcc aatcgtctcc tcaagcgcgt gcgcgatttt gcccagataa tggggaatgg
     3241 ggttatcgat gatgttatta ccgataaggc tttgactatg ctggatgttg accatgaagg
     3301 tttggactat gtggatcaaa aaatccttcg caccatgatt gagatgtacg gtggtggccc
     3361 tgtcggatta ggaactcttt ctgtgaatat tgccgaggag cgtgagactg tagaagacat
     3421 gtatgagcct tacttgattc aaaaaggttt tatcatgcgg acacggtctg gacgggtggc
     3481 gactgctaag gcatatgagc acttaggtta tgaatacagt gaaaaataag caagaaatct
     3541 tagaggcttt tagagaaaat ccagatatga tggccattct gacgatcatc cgagaccttg
     3601 gtctgaaaga ctcgtggttg gcagcaggtt ctgtcagaaa tttcatttgg aatcttttgt
     3661 cagacaaatc cccttttgat catgaaacag atatagatgt gattttcttt gatccagatt
     3721 tttcttatga ggaaacctta ttactggaga aaaagctgcg agaggatttt cctcagtacc
     3781 agtgggaatt gaaaaatcag gtctatatgc atcagcacag tcctcacact gcttcctata
     3841 ccagttctcg tgatgctatg agtaagtatc cagaacggtg tacgacagtt ggactgcgct
     3901 tgaatgaaga atcagatttt gaactctatg caccttatgg tttggaggat attttgaatt
     3961 ttcaagttcg tccaactcct catttcttag aaaatgaaga ccgaatggaa ctctatcaaa
     4021 cacgtttatc caagaaaaat tggcaggaga aatggaaaaa tttgattttt aaaaatactt
     4081 aaggaaactt taagctaggg agtgtacact aagttcataa gttaagaaga ccttaactta
     4141 aactcctaaa actttttcat aataatctcc ctataaaaat aaagtcgccc aatcaggcgg
     4201 cttatttttt tgaaaaatgg gcttggtgcc tgagaataaa tagcttagtg atagaagaaa
     4261 atggggaaat atggtataat gaaacgatag atttttgaat aggaataaga tcatgtttgg
     4321 attttttaag aaagataagg ctgtggaagt agaggttccg acacaggttc ctgctcatat
     4381 cggcatcatc atggatggca atggccgttg ggctaaaaaa cgtatgcaac cgcgagtttt
     4441 tggacataag gcgggcatgg aagcattgca aaccgtgacc aaggcagcca acaaactggg
     4501 cgtcaaggtt attacggtct atgctttttc tacggaaaac tggacccgtc cagatcagga
     4561 agtcaagttt atcatgaact tgccagtaga gttttatgat aattatgtcc cggaactaca
     4621 tgcgaataat gttaagattc aaatgattgg ggagacagac cgcctgccta agcaaacctt
     4681 tgaagcttta accaaggctg aggaattgac taagaacaac acaggattga ttcttaattt
     4741 tgctcttaac tatggtggac gtgctgagat tacacaggcg cttaagttga tttcccagga
     4801 tgttttagat gccaaaatca acccaggtga catcacagag gaattgattg gcaactatct
     4861 ctttacccag catttgccta agtacttacg agacccagac ttgattatcc gtactagtgg
     4921 agaattacgt ttgagcaatt tccttccatg gcagggagcc tatagtgagc tttattttac
     4981 ggacacctta tggcctgatt ttgacgaagt ggccttgcag gaagctattc ttgcctataa
     5041 tcgtcgtcat cgccgatttg gaggagttta ggaggaaata tgacccagga tttacagaaa
     5101 agaaccttgt ttgcagggat tgccctggct attttcctac caattttaat gattgggggc
     5161 ctcttgcttc agatagcaat cggaatcata gccatgctag ccatgcatga acttttgaag
     5221 atgagaggtc tagagaccat gacgatggag ggcctcttga ccctctttgc aacctttgca
     5281 ttgaccattc ccttggagaa ttacctgact tttttgccag ttgatgggaa tgtggttgcc
     5341 tatagtgttt tgatttcaat catgttagga acgaccgttt ttagcaagtc ttatacgatt
     5401 gaggatgcgg ttttccctct tgctatgagc ttctacgtgg gctttggatt taatgcttta
     5461 ctagatgctc gtgttgcagg tttggacaag gctctcttag ccttgtgtat cgtctgggcg
     5521 acagacagtg gtgcctatct tgttgggatg aactatggga aacgaaagtt agcaccaagg
     5581 gtatcgccta ataaaaccct tgagggtgcc ttgggtggta ttttaggagc aattttagta
     5641 accattatct ttatgatagt tgacagtaca gttgctcttc catatggaat ttacaagatg
     5701 tcagtctttg ctattttctt tagcattgct ggacaatttg gtgatttact agaaagttcg
     5761 atcaaacgtc attttggtgt taaggattct gggaaattta tccctggaca tggtggtgtt
     5821 ttggatcgtt tcgatagtat gttgcttgta tttccaatca tgcacttatt tggactcttt
     5881 taatcaaaag acggaggaaa cgctatgctc ggaattttaa cctttattct ggttttcggg
     5941 attattgtag tggtgcacga gttcgggcac ttctactttg ccaagaaatc agggatttta
     6001 gtacgtgaat ttgccatcgg tatgggacct aaaatttttg ctcacattgg caaggatgga
     6061 acggcctata ccattcgaat cttgcctctg ggtggctatg tccgcatggc cggttggggt
     6121 gatgatacaa ctgaaatcaa gacaggaacg cctgttagtt tgacacttgc tgatgatggt
     6181 aaggttaaac gcatcaatct ctcaggtaaa aaattggatc aaacagccct ccctatgcag
     6241 gtgacccagt ttgattttga agacaagctc tttatcaaag gattggttct ggaagaagaa
     6301 aaaacatttg cagtggatca cgatgcaacg gttgtggaag cagatggtac tgaggttcgg
     6361 attgcacctt tagatgttca atatcaaaat gcgactatct ggggcaaact gattaccaat
     6421 tttgcaggtc ctatgaacaa ttttatctta ggtgtcgttg ttttttgggt tttaatcttt
     6481 atgcagggtg gtgtcagaga tgttgatacc aatcagttcc atatcatgcc ccaaggtgcc
     6541 ttggccaagg taggagtacc agaaacggca caaattacca agattggctc acatgaggtt
     6601 agcaactggg aaagcttgat ccaagctgtg gaaacagaaa ccaaagataa gacggcaccg
     6661 actttggatg tgactatttc tgaaaagggg agtgacaaac aagtcactgt tacacccgaa
     6721 gatagtcaag gtcgttacct tctaggtgtt caaccggggg ttaagtcaga ttttctatcc
     6781 atgtttgtag gtggttttac aactgctgct gactcagctc tccgaattct ctcagctctg
     6841 aaaaatctga ttttccaacc ggatttgaac aagttgggtg gacctgttgc tatctttaag
     6901 gcaagtagtg atgctgctaa aaatggaatt gagaatatct tgtacttctt ggcaatgatt
     6961 tccatcaata ttgggatttt taatcttatt ccgattccag ccttggatgg tggtaagatt
     7021 gtgctcaata tcctagaagc catccgccgc aaaccattga aacaagaaat tgaaacctat
     7081 gtcaccttgg ccggagtggt catcatggtt gtcttgatga ttgctgtgac ttggaatgac
     7141 attatgcgac tcttttttag ataatcgagg aatattatga aacaaagtaa aatgcctatc
     7201 ccaacgcttc gcgaaatgcc aagcgatgct caagttatca gccatgctct tatgttgcgt
     7261 gctggttatg ttcgccaagt ttcagcaggt gtttattctt atctaccact tgccaaccgt
     7321 gtgattgaaa aagctaaaaa catcatgcgc caagaattcg aaaagattgg tgctgttgag
     7381 atgttggctc cagcccttct tagtgcagaa ttgtggcgtg aatcaggtcg ttacgaaacc
     7441 tatggtgaag acctttacaa actaaaaaac cgtgaaaaat cagactttat cttaggtcca
     7501 actcacgaag aaacctttac agctattgtc cgtgattctg ttaaatctta caagcaattg
     7561 ccactcaacc tttatcaaat tcagcccaag tatcgtgatg aaaaacgccc gcgtaatgga
     7621 cttcttcgta cacgtgagtt tatcatgaag gatgcttata gtttccacgc taactatgat
     7681 agtttggata gtgtttatga tgagtacaaa gcagcttatg agcgtatttt cactcgtagt
     7741 ggtttagact tcaaggctat tattggtgac ggtggagcca tgggtggtaa ggatagccaa
     7801 gaatttatgg ccattacatc tgctcgtaca gaccttgacc gctgggttgt cttggacaag
     7861 tcagttgcct catttgacga aattcctgca gaagtgcaag aagaaatcaa ggcagaattg
     7921 ctcaaatgga tagtctctgg tgaagatacc attgcttact caagtgagtc tagctatgca
     7981 gctaacttag aaatggcaac aaacgagtac aaaccaagca accgtgttgt cgctgaagaa
     8041 gaagttactc gtgttgcaac gccagatgtt aaatcaattg atgaagttgc agccttcctc
     8101 aatgttccag aagaacaaac gattaaaacc ctcttctaca ttgcagatgg tgagcttgtt
     8161 gcagcccttc tagttggaaa tgaccaactc aacgaagtca agttgaaaaa ccacttagga
     8221 gcagatttct ttgacgttgc tagcgaagaa gaagtggcga atgttgttca agcaggattt
     8281 ggttcacttg gaccagttgg tttgccagag aatattaaaa ttattgcaga tcgtaaggtg
     8341 caagatgttc gcaatgcagt tgtcggtgct aacgaagatg gctaccactt gactggtgtg
     8401 aacccaggcc gtgattttac tgcagaatat gtggatatcc gtgaagttcg tgagggtgaa
     8461 atttccccag atggacaagg tgtccttaac tttgcgcgtg gtattgagat cggtcatatt
     8521 ttcaaactcg gaactcgcta ttcagcaagc atgggagcag atgtcttgga tgaaaatggt
     8581 cgtgctgtgc caatcatcat gggatgttac ggtatcggtg tcagccgtct tctttcagca
     8641 gtgatggagc aacacgctcg cctctttgtt aacaaaacgc caaaaggtga ataccgttac
     8701 gcttggggaa tcaatttccc taaagaattg gcaccatttg atgtgcattt gattactgtc
     8761 aatgtcaagg atgaagaagc gcaagccttg acagaaaaac ttgaagcaag cttgatggga
     8821 gctggttatg aagtcttgac agatgaccgt aacgaacgtg tcggtgttaa atttagcgat
     8881 agtgacttga ttggtcttcc aatccgtatc actgttggta aaaaagcggc cgatggcatt
     8941 gtagaagtta agatcaaggc aactggtgac accattgagg ttcatgcaga taacgtgctt
     9001 gaaacgcttg aaatcctaag caagaaataa aaactataat cagaagaaaa acaaggaaaa
     9061 aatgtaacta gttttta
//
LOCUS       DBERDK010000074         9027 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.074, whole genome shotgun sequence.
ACCESSION   DBERDK010000074 DBERDK010000000
VERSION     DBERDK010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 9027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9027
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.074"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            180..1625
                     /gene="cps4A"
                     /locus_tag="ACL73L_001751"
     CDS             180..1625
                     /gene="cps4A"
                     /locus_tag="ACL73L_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4A"
                     /protein_id="HGS0772682.1"
                     /translation="MSRRFKKSGSQKVKRSVNIVLLTIYLLLVCFLLFLIFKYNILAF
                     RYLNLVVTALVLLVALVGLLLIIYKKAEKFTIFLLVFSILVSSVSLFAVQQFVGLTNR
                     LNATSNYSEYSISVAVLADSEIENVTQLTSVAAPTGTDNENIQKLLADIKSSQNTDLT
                     VNQSSSYLAAYKSLIAGETKAIVLNSVFENIIESEYPDYASKIKKIYTKGFTKKVEAP
                     KTSKNQSFNIYVSGIDTYGPISSVSRSDVNILMTVNRDTKKILLTTTPRDAYVPIADG
                     GNNQKDKLTHAGIYGVDSSIHTLENLYGVDINYYVRLNFTSFLKMIDLLGGIDVHNDQ
                     EFSALHGKFHFPVGNVHLDSEQALGFVRERYSLADGDRDRGRNQQKVIVAILQKLTST
                     EALKNYSTIIDSLQDSIQTNMPLETMINLVNAQLESGGTYKVNSQDLKGTGRMDLPSY
                     AMPDSNLYVMEIDDSSLAVVKAAIQDVMEGR"
     gene            1627..2358
                     /gene="cps4B"
                     /locus_tag="ACL73L_001752"
     CDS             1627..2358
                     /gene="cps4B"
                     /locus_tag="ACL73L_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4B"
                     /protein_id="HGS0772683.1"
                     /translation="MIDIHSHIVFDVDDGPKSREESKALLAEAYRQGVRTIVSTSHRR
                     KGMFETPEEKIAENFLQVREIAKEVADDLVIAYGAEIYYTLDALEKLEKKEIPTLNDS
                     RYALIEFSMHTSYRQIHTGLSNILMLGITPVIAHIERYNALENNEKRVRELIDMGCYT
                     QINSYHVSKPKFFGEKYKFMKKRARYFLERDLVHVVASDMHNLDSRPPYMQQAYDIIA
                     KKYGAKKAKELFVDNPRKIIMDQLI"
     gene            2367..3059
                     /gene="cpsC"
                     /locus_tag="ACL73L_001753"
     CDS             2367..3059
                     /gene="cpsC"
                     /locus_tag="ACL73L_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CpsC"
                     /protein_id="HGS0772684.1"
                     /translation="MKEQNTLEIDVLQLFRVLWKRKLVILLVAIITSSVAFAYSTFVI
                     KPEFTSTTRIYVVNRNQGEKSGLTNQDLQAGSYLVKDYREIILSQDVLEEVVSDLKLD
                     LTPKGLANKIKVTVPADTRIVSVSVNDRVPEEASRIANSLREVAVKKIISITRVSDVT
                     TLEEARPAISPSSPNLKRNTLIGFLAGVIGTSVIVLLLELLDTRVKRPEDIEDTLQMT
                     LLGVVPNLNKLK"
     gene            3069..3752
                     /gene="cpsD"
                     /locus_tag="ACL73L_001754"
     CDS             3069..3752
                     /gene="cpsD"
                     /locus_tag="ACL73L_001754"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase
                     CpsD"
                     /protein_id="HGS0772685.1"
                     /translation="MPTLEIAQKKLEFIKKAEEYYNALCTNIQLSGDKLKVISVTSVS
                     PGEGKTTTSVNIAWSFARAGYKTLLIDGDIRNSVISGFFKSREKITGLTEFLSGTADL
                     SHGLCDTNIENLFVVQSGSVSPNPTALLQSKNFNDMIETLRKYFDYIIVDTAPIGIVI
                     DAAIITQKCDASILVTATGEANKRDVQKAQQQLKQTGKLFLGVVFNKLDISVDKYGVY
                     GFYGNYGKK"
     gene            3768..5135
                     /locus_tag="ACL73L_001755"
     CDS             3768..5135
                     /locus_tag="ACL73L_001755"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HGS0772686.1"
                     /translation="MDEKGLKIFLAVLQSIIVILLVYFLSFVRETELERSSMVILYLL
                     HFFVFYFSSYGNNFFKRGYLVEFNSTIRYIFFFAIAISVLNFFIAERFSISRRGMVYF
                     LTLEGISLYLLNFLVKKYWKHVFFNLKNSKKILLLTVTKNMEKVLDKLLESDELSWKL
                     VAVSVLDKSDFQHDKIPVIEKEKIIEFATHEVVDEVFVNLPGESYDIGEIISRFEKMG
                     MDVTVNLKAFDKNLGRNKQIHEMVGLNVVTFSTNFYKTSHVISKRILDICGATIGLIL
                     FAIVSLVLVPLIRKDGGPAIFAQTRIGKNGRHFTFYKFRSMQIDAEAIKEQLMDQNTM
                     QGGMFKIDNDPRVTKIGRFIRKTSLDELPQFWNVFIGDMSLVGTRPPTVDEYDQYTPE
                     QKRRLSFKPGITGLWQVSGRSKITDFDDVVKLDVAYIDNWTIWKDIEILLKTVKVVFM
                     RDGAK"
     gene            5166..6338
                     /gene="cps2T"
                     /locus_tag="ACL73L_001756"
                     /gene_synonym="wchF"
     CDS             5166..6338
                     /gene="cps2T"
                     /locus_tag="ACL73L_001756"
                     /gene_synonym="wchF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta 1-4 rhamnosyltransferase Cps2T"
                     /protein_id="HGS0772687.1"
                     /translation="MKKSVYIIGSKGIPAKYGGFETFVEKLTAFQQDKTIQYYVACMR
                     ENSAKSGTTEDVFEHNGAICYNVDVPNIGPARAIVYDIAAINRAIKIAKENKDEDPIF
                     YILACRIGPFIHGIKKKIQEIGGTLLVNPDGHEWLRAKWSAPVRRYWKISEGLMVKHA
                     DLLVCDSKNIEKYIQEDYIQYQPKTTYIAYGTDTTRSTLNSSDEKVRAWFKEKNVSEN
                     EYYLVVGRFVPENNYESMIRGFLASSSKKDFVLITNVEQNKFYNQLLASTGFDKDPRV
                     KFVGTVYDQELLKYIRENAFAYFHGHEVGGTNPSLLEALASTKLNLLLDVGFNREVGE
                     QSAIYWKKDELSQVIEKVEQFDAKIIDELDRQSNQRIVDFFTWEKIVTDYEKLFKK"
     gene            6335..7606
                     /locus_tag="ACL73L_001757"
     CDS             6335..7606
                     /locus_tag="ACL73L_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HGS0772688.1"
                     /translation="MKILHYTLGFQPQRTGGLVKYAEDLMLEQIAQGYQVAALYPGRI
                     KFFSKKIEIIKETSRQFECYELLNSLPMALFGGISDPTAFLTPCDKNVYRTFLEKVQP
                     DIIHIHSFMGLHKEFLEIAKSLNIRVVFTSHDYYGLAPVPHFYFNGVDYSDKSTNLTW
                     NIMSSNALSVKKLRLFQVSFYPTIRKLLKLLGKNPKSKKNLVIRDVIEEQDYSELRYY
                     YNEMFHLIDGYLFNSRLAKKVYEINEIKSANSIVLSITNSSINHHQRLTTTNNKIRVA
                     YIGPDEEYKGYFDFVDFVETLDRESYEVATYGHLPNEECPSFIEQKGYFTKEMIDSVY
                     ENIDILIVPSKWKETFGLITVEALSYGVNVFVSENVGSKDLLPESHVFKNQNDLIVKF
                     LKNDIENTKLKTLDEHSIEVIQYYERVINDS"
     gene            7596..8282
                     /locus_tag="ACL73L_001758"
     CDS             7596..8282
                     /locus_tag="ACL73L_001758"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HGS0772689.1"
                     /translation="MIVKSFIKKITGKTVDIHPDVPLSYILQRGINYSFGLFRGVVRG
                     IGFGQNDKRLFIGQGVSILAKRKLFVGKNVRIGKKVSIDALSKEGIHFADNVKIGDYS
                     QIIGTGSIKNMGIGLKIGKNSSFSEYSLFGSAGGITIGDNVIAGQNVRFHAENHNYSD
                     LDKLIVEQGVSRKGISVGDNCWIGAGSVFLDGSSIGSGCVVAANSVITKNFPDNVIVA
                     GVPAKIIKMK"
     gene            8294..>9027
                     /locus_tag="ACL73L_001759"
     CDS             8294..>9027
                     /locus_tag="ACL73L_001759"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGS0772690.1"
                     /translation="MRKEIKAVYAILNYNTWEDTARLAQKVATFQHIQSVIIVDNLST
                     DDSYHYLKRLEGEKISVYQTQRNGGYSVGNNFAARKAYNMGVDILFISNPDVDIDEKD
                     SLMIAQNLYKNSSYALLSGIEYNAMKEIDLPIVWHENSYYDDLLDCLFLTRKWRRKKE
                     DINLSMIKSSIIDVDLVKGSFFAVRLSDFHDVGYFDESVFLFCEERILAKKLQKVNKK
                     IGILPEAKYYHNHSTSINERYKKKKE"
BASE COUNT         3022 a         1292 c         1834 g         2879 t
ORIGIN      
        1 ttagaaatcg atttgactgt cctgatcgat ttgtcatgtt cttatttcat tttactatat
       61 ttttggttcg cgggaagtct actaagatac ttaaagatgc agatagtgaa aataaaggtg
      121 tagacattac cgtaaaaaag tgatataatc gtatgatgtt caatgtatag gtgttaatca
      181 tgagtagacg ttttaaaaaa tcaggttcac agaaagtgaa gcgaagtgtt aatatagttt
      241 tgttgactat ttatttattg ttagtttgtt ttttattgtt cttaatcttt aagtacaata
      301 tccttgcttt tagatatctt aatctagtgg taactgcgtt agtcctacta gttgccttgg
      361 tagggctact cttgattatc tataaaaaag ctgaaaaatt tactattttt ctgttggtgt
      421 tctctatcct tgtcagctct gtgtcgctct ttgcagtaca gcagtttgtt ggactgacca
      481 atcgtttaaa tgcgacttct aattactcag aatattcaat cagtgtcgct gttttagcag
      541 atagtgagat cgaaaatgtt acgcaactga cgagtgtggc agcaccgact gggactgata
      601 atgaaaatat tcaaaaacta ctagctgata tcaagtcaag tcagaatacc gatttgacgg
      661 tcaaccagag ttcgtcttac ttggcagctt acaagagttt gattgcaggg gagactaagg
      721 ccattgtcct aaatagtgtc tttgaaaata tcatcgagtc agagtatcca gactacgcat
      781 cgaagataaa gaagatttat accaagggat tcactaaaaa agtagaagct cctaagacgt
      841 ctaagaatca gtctttcaat atctatgtta gtggaattga cacctatggt cctattagtt
      901 cggtgtcgcg atcagatgtc aatatcctga tgactgtcaa tcgagatacc aagaaaatcc
      961 tcttgaccac aacaccacgt gatgcctatg taccaatcgc agatggtgga aataatcaaa
     1021 aagataaatt aacccatgcg ggcatttatg gagttgattc gtccattcac accttagaaa
     1081 atctctatgg agtggatatc aattactatg tgcgattgaa cttcacttct ttcttgaaaa
     1141 tgattgactt attgggaggg atagatgttc ataatgatca agagttttca gctctacatg
     1201 ggaagttcca tttcccagta gggaatgtcc atctagactc tgagcaagct ttaggttttg
     1261 ttcgtgagcg ctactcccta gcagatggcg atcgtgaccg tggtcgcaac caacaaaagg
     1321 tgattgtggc tatccttcaa aaattaacgt caaccgaagc actgaaaaat tatagtacga
     1381 tcattgatag cttgcaagat tctatccaaa caaatatgcc acttgagacc atgataaatt
     1441 tggtcaatgc tcagttagaa agtggtggaa cttacaaagt gaattcgcaa gacttgaaag
     1501 gtacaggtcg gatggatctt ccttcttatg caatgccaga cagtaacctc tatgtgatgg
     1561 aaatagatga tagtagttta gctgtagtta aagcagctat acaggatgtg atggagggta
     1621 gatgaaatga tagacatcca ttcgcatatc gtttttgatg tagatgacgg tcccaaatca
     1681 agagaggaaa gcaaggctct cttggcagaa gcctacagac agggggtgcg aaccattgtc
     1741 tctacctctc accgtcgcaa gggcatgttt gaaactccgg aagagaagat agcagaaaac
     1801 tttcttcagg ttcgggaaat tgcaaaagaa gtagcagatg atttagtcat tgcttatggc
     1861 gcagagatat actatactct ggatgctcta gaaaagctag aaaaaaaaga aattcctacc
     1921 cttaatgata gtcgttatgc cttgattgag tttagcatgc atacttccta tcgtcagatt
     1981 catacgggat tgagcaatat tttgatgttg ggaatcacgc cagtaattgc tcatattgaa
     2041 cgttataatg ctttagagaa taacgaaaaa cgtgttcgtg aactgattga tatggggtgc
     2101 tatactcaga taaatagtta tcatgtttca aaacctaagt tctttggtga aaaatataaa
     2161 ttcatgaaaa agagagctcg gtattttttg gaacgtgatt tagttcatgt agttgcaagt
     2221 gacatgcaca atttagacag tagacctcca tatatgcaac aggcatatga tatcattgct
     2281 aagaaatatg gagcgaaaaa agcgaaagaa ctttttgtag ataatcccag aaaaattata
     2341 atggatcaat taatttagga gaaaatatga aggaacaaaa cactttggaa atcgatgtat
     2401 tgcaactatt cagagtttta tggaaaagga agttggtcat tttattagtg gcaattataa
     2461 cttcttcagt tgcttttgcc tacagtactt ttgttatcaa acctgagttt actagtacga
     2521 ctcggattta tgtagttaac cgtaatcagg gagagaagtc tggtttaacc aatcaagact
     2581 tgcaggcagg atcgtacttg gttaaagact atcgtgaaat tatcctatcg caggatgttt
     2641 tggaggaagt tgtttctgat ttgaaactag atttgacgcc aaaaggtttg gctaataaaa
     2701 tcaaagtaac agtaccagct gatacccgta ttgtctctgt ttcagttaat gatcgagttc
     2761 ctgaagaggc aagccgtatc gctaactctt tgagagaagt agctgttaaa aaaattatca
     2821 gtattactcg tgtttctgat gtgacaacac tggaggaggc aagaccggcg atatcaccgt
     2881 cttcgccaaa tcttaaacgc aatacactaa ttggtttttt ggcaggggtg attggaacta
     2941 gtgttatagt tcttcttctt gaacttttgg acactcgtgt gaaacgtccg gaagatatcg
     3001 aagatacact acagatgaca cttttgggag ttgtaccaaa cttgaataag ttgaaatagg
     3061 agagagagat gccgacatta gaaatagcac aaaaaaaact ggagttcatt aagaaggcag
     3121 aagaatatta caatgccttg tgtacaaata tacagttgag cggagataaa ctaaaagtaa
     3181 tttccgttac ttctgttagc ccgggggaag gaaaaacaac tacttccgta aatatagcat
     3241 ggtcgtttgc gcgtgcaggc tataaaactc ttttgatcga tggcgatatt cgaaattcag
     3301 tcatatcagg attttttaaa tctcgtgaaa aaattacagg gctaacagaa tttttatctg
     3361 ggacagctga tttatctcac ggtttatgtg atacaaatat tgaaaattta tttgtagttc
     3421 aatcgggctc tgtatcacca aaccctacag ccttgttaca aagtaaaaat tttaatgata
     3481 tgattgaaac attgcgtaaa tattttgatt atatcattgt tgatacagca cctattggaa
     3541 ttgttattga tgcggcaatt atcactcaaa agtgtgatgc gtccatcttg gtaacagcaa
     3601 caggtgaggc gaataaacgt gatgtccaaa aagcgcaaca acaattaaaa caaacaggga
     3661 aactgttcct aggagttgtt tttaataaat tagatatctc ggttgataag tatggagttt
     3721 acggtttcta tggaaattat ggtaaaaaat aacttaggaa agattttatg gatgaaaaag
     3781 gattgaaaat ttttctggca gtattacaga gtattattgt cattttattg gtttattttc
     3841 ttagctttgt tagagagaca gaacttgaac gttcttcgat ggttatacta taccttctcc
     3901 acttttttgt attctatttt agttcctatg gtaacaattt ttttaaaaga gggtacctag
     3961 ttgagtttaa tagtactata agatatattt ttttctttgc aatagctata agtgtattaa
     4021 acttttttat agcggaacgg tttagcatct ctagaagagg aatggtatac ttcttaactt
     4081 tagaaggaat atccttatac ttgttaaatt tcttagtaaa gaaatattgg aagcatgtgt
     4141 tttttaatct aaaaaatagc aagaaaattt tactgttaac agtaacgaaa aatatggaaa
     4201 aagttcttga taaattgcta gaatctgatg aactttcatg gaaattggta gcagtaagtg
     4261 ttttggataa atctgatttt caacatgata aaatacctgt aattgaaaag gaaaaaatta
     4321 ttgaatttgc aacgcatgaa gttgtggatg aggtgtttgt caatcttcca ggagagagct
     4381 acgatattgg agaaattatc tctaggtttg agaaaatggg gatggatgta actgtaaatc
     4441 ttaaagcatt tgataagaat ttgggtcgca ataaacaaat tcatgagatg gtaggattga
     4501 atgtagtcac tttctctaca aatttttata aaactagtca tgtgatttca aagagaattc
     4561 tcgatatttg tggtgccact attggcctta ttctttttgc tatagttagt ctagttttag
     4621 ttccattgat tcgtaaagat ggcggaccag ctatttttgc tcaaactcgt atagggaaaa
     4681 atggtcgaca ttttaccttt tataaattcc gttcgatgca gatcgatgct gaagctatca
     4741 aagaacagtt gatggatcaa aatacgatgc aaggtggtat gtttaagata gacaatgatc
     4801 ctcgtgttac aaaaattggt cgctttattc gtaaaaccag tttagatgag ttaccccagt
     4861 tttggaatgt ctttatagga gatatgagtt tggtgggaac acgtccacct acagtagacg
     4921 agtatgatca gtatactcca gaacagaaac gtcgactcag ctttaaaccc ggtattacag
     4981 gtttatggca ggttagcggc cgtagtaaaa taaccgattt tgacgatgtt gtaaaattag
     5041 atgtggctta tattgataat tggacaatct ggaaagatat tgaaattttg cttaaaactg
     5101 ttaaagttgt atttatgaga gatggagcga agtaatttct gtatatccat catattagga
     5161 gagaaatgaa aaagtcagtt tatatcattg gttcaaaagg aattcctgct aagtatggag
     5221 gatttgaaac ttttgttgaa aaattaacag ccttccaaca agataagact atccaatatt
     5281 atgtggcttg tatgcgtgaa aactctgcaa aatcagggac tactgaggat gtttttgaac
     5341 ataatggtgc tatctgttat aacgtcgatg ttcctaatat tggtccagcg cgagccatag
     5401 tatatgatat cgcggcaatt aacagagcta ttaaaattgc caaagaaaat aaggatgaag
     5461 atccaatctt ctatattcta gcttgtcgaa tcggtccgtt catccatgga attaagaaaa
     5521 aaatccagga gataggtggg actcttctag ttaatccaga tggtcacgag tggttacgag
     5581 ctaagtggag cgctccagtt cgtcgttatt ggaaaatttc cgaaggtctt atggtcaaac
     5641 atgcagatct tttagtgtgt gatagtaaga atattgaaaa atatatccaa gaagattata
     5701 tacagtatca acctaagaca acctatatcg cttatgggac agatacaaca cgctccacat
     5761 taaatagcag tgatgaaaaa gtgcgtgctt ggttcaagga gaaaaatgtt tctgaaaacg
     5821 agtattacct agttgtagga cgttttgttc cagaaaataa ttatgagtct atgatacgtg
     5881 gtttcttggc gtctagctct aagaaggatt ttgtcctaat cacaaatgta gaacagaata
     5941 aattttataa tcagctatta gcaagtactg gatttgataa agatccacga gtgaaatttg
     6001 ttggaacagt ctacgaccaa gaacttctga agtatattcg agaaaatgct tttgcttact
     6061 tccacggaca tgaagttggt ggaacaaatc cttcgttact tgaagcatta gcatccacaa
     6121 agttaaactt actactcgat gttggtttta accgcgaagt tggggagcaa agtgcgatct
     6181 attggaaaaa agatgagctt tcccaagtaa tcgagaaagt tgaacaattt gatgcaaaaa
     6241 ttattgatga gttagataga caatcaaatc agagaattgt ggattttttc acttgggaaa
     6301 agattgtcac agactacgag aaattattta aaaaatgaaa atattacatt atactttagg
     6361 atttcaaccc cagcgaacag gtggtttggt taaatatgct gaggatttaa tgctagagca
     6421 aattgctcaa ggttatcaag tggctgccct atatccagga agaattaagt tcttttcaaa
     6481 aaaaatagaa ataattaaag agacgtcaag acagtttgag tgttatgagc tgcttaatag
     6541 cttacctatg gctttatttg gagggatatc cgatccaact gctttcctga caccttgtga
     6601 caaaaatgtt tatcgtactt ttttagaaaa agtacaaccc gacattatac atattcactc
     6661 gtttatggga ttgcataaag aattcctcga aattgctaaa agcttgaata ttagagtggt
     6721 ttttacgagc catgattatt atggattagc acccgtacct catttctatt ttaatggggt
     6781 ggattatagt gataaaagta caaacttaac atggaatatt atgtcttcca atgctttaag
     6841 tgtgaaaaaa ctaagacttt ttcaggtttc attttatcct actattcgta aacttttgaa
     6901 actattaggg aaaaatccaa aatctaaaaa gaatttagtt attcgagatg ttattgagga
     6961 gcaagattat agtgagcttc gatactatta taatgagatg tttcacttaa tagatggtta
     7021 tttgtttaat agtagacttg caaagaaggt atatgagata aatgagatta agtcagctaa
     7081 tagtatagta ttatctataa caaatagttc aattaatcat catcagagat tgacaactac
     7141 aaataataaa attagagttg cttatattgg tccagatgaa gaatataaag gatattttga
     7201 ttttgttgac tttgttgaaa ctttagatcg agagtcctat gaagtggcaa cttatggcca
     7261 tttaccaaat gaagagtgtc cttcattcat tgaacaaaaa ggatatttta ctaaggaaat
     7321 gattgacagt gtctatgaaa atattgatat tcttattgtt ccaagtaagt ggaaggaaac
     7381 atttggattg ataacagtag aagcattatc ctatggggta aatgttttcg tgagtgaaaa
     7441 tgttgggtca aaagacttac ttccagaatc gcatgttttt aaaaatcaga atgatttaat
     7501 agttaaattt ttaaaaaatg atatagaaaa tacaaaattg aaaactttag atgaacactc
     7561 gatagaagtg attcagtatt atgaaagagt gataaatgat agttaagagt ttcataaaaa
     7621 agattactgg aaaaacagta gacattcatc cagatgtacc tttgtcgtat atactacaaa
     7681 gaggtataaa ctatagtttc ggactctttc gtggggttgt acgtggaatc ggatttggtc
     7741 aaaacgacaa acgtttattc attggacaag gagtttctat cttagcaaag cgtaaattat
     7801 ttgttggaaa aaatgttcga attggtaaaa aggtgtctat agatgcactg tcaaaagaag
     7861 gaatccattt tgcagataat gtaaagattg gagattattc tcaaattatt ggcacaggtt
     7921 ctataaaaaa tatggggatt ggcttgaaaa tcggtaagaa ctcatctttt tcagaatata
     7981 gtttatttgg ttctgctgga ggtatcacaa ttggtgataa tgttatcgca ggtcagaatg
     8041 ttcgttttca tgctgaaaat cataattata gtgatttaga taaactcatt gttgaacaag
     8101 gggtatctcg aaagggaatt tcagtaggtg ataattgttg gattggtgct ggatctgttt
     8161 ttttggatgg gtccagcatt ggttcaggtt gtgttgtagc tgcaaattct gtaattacga
     8221 aaaactttcc agataacgtt attgttgctg gagtaccagc taaaattata aaaatgaaat
     8281 gataggatgt taaatgagga aagaaattaa agctgtttat gcgatattaa attacaatac
     8341 ttgggaagat actgcgcgtt tagcacagaa agttgcaaca tttcagcata ttcaatctgt
     8401 gataatagtg gataatctat caacagatga ttcataccat tatttaaaaa gacttgaggg
     8461 agagaaaatt tcagtttatc aaacacaaag aaatggtgga tattctgttg gaaataattt
     8521 tgcagcacga aaagcttata atatgggagt tgatattctt ttcatttcaa atccagacgt
     8581 tgatattgat gaaaaagatt ccttgatgat tgcacaaaat ttgtataaaa atagttcata
     8641 tgcactgcta tcaggtatag aatataatgc tatgaaggag atagatttgc caattgtctg
     8701 gcatgaaaat tcctattacg atgacttact tgattgtttg tttttaacca gaaaatggcg
     8761 aagaaaaaaa gaagatatca atttgtcaat gattaaatca tctataatag atgtagatct
     8821 tgttaaagga tcgttttttg ctgtaagact gtctgatttt catgatgttg gttattttga
     8881 tgaatctgta tttctttttt gtgaagagag aatattagct aaaaagttac aaaaagtaaa
     8941 taaaaaaata ggtattctgc ctgaagcgaa atattaccac aatcattcaa cttctataaa
     9001 tgagagatat aaaaagaaaa aagaaca
//
LOCUS       DBERDK010000075         9007 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.075, whole genome shotgun sequence.
ACCESSION   DBERDK010000075 DBERDK010000000
VERSION     DBERDK010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 9007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9007
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.075"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            86..538
                     /locus_tag="ACL73L_001760"
     CDS             86..538
                     /locus_tag="ACL73L_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HGS0772691.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVHSALRNDAKLTIAHVIDT
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            complement(573..1961)
                     /locus_tag="ACL73L_001761"
     CDS             complement(573..1961)
                     /locus_tag="ACL73L_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HGS0772692.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKIVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFEDLKLTRSNPFASDVINQGNSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRAGFKIEELVEFVR
                     AASPSEEDFGQAVSQLHQALDKAADKVAKKTPAQQDLIGQVDALIDMLYFTYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELQRQL
                     KAYERHKERNKS"
     gene            2027..2989
                     /locus_tag="ACL73L_001762"
     CDS             2027..2989
                     /locus_tag="ACL73L_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HGS0772693.1"
                     /translation="MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HALDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
                     TTNVNTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQALKDYMEG"
     gene            complement(3018..3221)
                     /locus_tag="ACL73L_001763"
     CDS             complement(3018..3221)
                     /locus_tag="ACL73L_001763"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772694.1"
                     /translation="MNTIERTRRLVKGCATHCFEVVDRTDEVSSKHCFEVADRTDEVS
                     SKHVFEVVDRTDEVSNHTYGKAT"
     gene            complement(3285..4295)
                     /gene="ccpA"
                     /locus_tag="ACL73L_001764"
     CDS             complement(3285..4295)
                     /gene="ccpA"
                     /locus_tag="ACL73L_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HGS0772695.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLAKENERIAFVSGPLVDDINGKVRLVGYKETLKKAGIAYSEGL
                     VFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
     gene            complement(4720..5319)
                     /locus_tag="ACL73L_001765"
     CDS             complement(4720..5319)
                     /locus_tag="ACL73L_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGS0772696.1"
                     /translation="MKVLVAEDQSMLRDAMCQLLTLQPDVESVLQAKNGQEAIQLLEK
                     ESVDIAILDVEMPVKTGLEVLEWIRSERLETKVVVVTTFKRAGYFERAVKAGVDAYVL
                     KERSVADLMQTLHTVLEGRKEYSPELMEVVMTRPNPLTEQEIAVLKGIARGLSNQEIA
                     DQLYLSNGTIRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            complement(5321..6418)
                     /locus_tag="ACL73L_001766"
     CDS             complement(5321..6418)
                     /locus_tag="ACL73L_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGS0772697.1"
                     /translation="MLERLKSIHYMFWASLIFMIFPILPVVTGELPAWHLLVDILFVV
                     TYLGVLITKNQRISWLFWGIMLVYVAGNTAFVAGNYIWFFFFLSNLLIYHFGVRSLKS
                     LHVWTFLLAQVLVVGRLLIFQRIEVEFLVYMLVILTFVDLMTLGSVRIRLVEDLKEAQ
                     VKQNTQINLLLAENERNRIGQDLHDSLGHTFAMLSVKTDLALQLFQMQAYPQVEKELR
                     EIQQISKESMREVRTIVENLKSRTLTSELETVKKMLEIAGIEVETDNQLDTASLTQEL
                     ESMASMILLELVTNIIKHAKASKAYLKLERTEKELILTVSDDGCGFAFLKGDELHTIR
                     DRVFPFSGEVSVISQKHPTEVQVRLPYKERN"
     gene            complement(6418..7155)
                     /locus_tag="ACL73L_001767"
     CDS             complement(6418..7155)
                     /locus_tag="ACL73L_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS0772698.1"
                     /translation="MKRWIALSKIEFLLTKRQLIYYLLSVGMPTAFYLFFSGIYQDTP
                     GELANFMRDYLISMTAFSMMSTTILSFPAVLHTDKMNNWQKTLRHSPVNMVEYYLSKI
                     TSMLVDYLVSILVVFTVGHFVRGVDMPLVKWVGAAILLIVGSIAFVALGLTLTLLPTS
                     QLMSVVGNLLYLGLAVLGGLWMPISLFPDWMQAVGKCLPTYQLMELLKTFLNEGGINL
                     SATVYLLVFSAVLFGLTIYLQGHKENA"
     gene            complement(7157..8041)
                     /locus_tag="ACL73L_001768"
     CDS             complement(7157..8041)
                     /locus_tag="ACL73L_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0772699.1"
                     /translation="MTVVKVEKLSKKIKDKEILRNISFEINDGECVALIGPNGAGKTT
                     LLDCLLGDKLVTSGQVSIQGLPVTSSKLDYTRAYLPQENVIVQKLKVKELIAFFQRIY
                     PNPLSNQEIDQLLQFVKQQKEQLAEKLSGGQKRLFSFVLTLIGRPKIVFLDEPTASMD
                     TSTRQRFWEIVQELKAQGVTILYSSHYIEEVEHTADRILLLNKGELIRDTTPLAMRSE
                     EIEKHFILPIAYKEVVEQSNLVENWTLKQDSLQVVTREADAFWELLAQAGCRMQEIEV
                     NNRSLLNTIFEETQKGDN"
     gene            complement(8028..8222)
                     /locus_tag="ACL73L_001769"
     CDS             complement(8028..8222)
                     /locus_tag="ACL73L_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772700.1"
                     /translation="MKRGIIYFFIGLSLLVWLVEMFTDWFDQALLRQFIRGALGFGFM
                     IFVVFLMGMEWLKGESHDGG"
     gene            complement(8219..8425)
                     /locus_tag="ACL73L_001770"
     CDS             complement(8219..8425)
                     /locus_tag="ACL73L_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772701.1"
                     /translation="MGEEEMRNKMIIAVSLVVAGVMTYLMFSGLDEDFYHFPWKVFAG
                     FGIMSWLVREGLKLVRDVKKEFEE"
BASE COUNT         2753 a         2055 c         1582 g         2617 t
ORIGIN      
        1 attcttttga aaaatacttt ttttgcttta cttctatagt gaaaaggttt acaattatag
       61 taagaaaatt tcaggaggtt tcattatggc acaacgttac caaaatatca tggtcgcaat
      121 cgatggttct aaggaagcgg acttggcttt tgtcaaggga gttcattctg ctctacgaaa
      181 cgacgctaaa ctcaccatcg cacatgtcat tgacacacgc gctctccaaa gcgtatccac
      241 ctttgatgct gaagtttacg aagaactcca agtcgacgct gaaagtctga tgaaagagta
      301 cgaaaaacgt gctaaagatg ctggggtagc agatgttcat atcgtcattg aaatgggaaa
      361 tccaaagacc ctgctagcac gtactattcc agatgccgag gaagtggacc tcatcctcgt
      421 tggcgcaact ggtctcaacg cctttgaacg cctcttggtc ggctcttcat ctgaatacat
      481 actccgccat gctaaggtcg atttgctggt tgtaagagaa caagaaaaaa ccttataatc
      541 acaaagaaaa ggagccccta gctccttttt gtttacgatt tatttctctc tttatggcgt
      601 tcgtaagcct tgagctggcg ctgcagttcc tttttaatag caggttctgg agcatatttt
      661 tcttcccaat tatctggttt taagatttta tgggtcactg gatcaaaatg agccttacca
      721 tctggaaaaa ttttccccat attggcctga tggacaatat caaaaatacg ttctgggtcc
      781 acccccatca agacaaaact gccgtaggtg aagtaaagca tgtcaatcaa ggcatccact
      841 tgccctatca aatcttgctg agcaggtgtc ttcttggcta ctttatctgc tgccttatca
      901 agggcctgat gaagttgcga tacagcttga ccaaaatctt cttcagaagg actggctgct
      961 cgaacaaact ccaccaattc ttctatttta aaaccagccc tatgggttgc accctctaaa
     1021 tcccaagctc gaggttcttc ttgggttcgt tcatccatca tgtggtggaa ggtcttgacc
     1081 ttattgaaat gatagtcacg gctgacaaag actttttctg aagacaaaac accaaaatgt
     1141 tccaaggcct tgtggatccc atcttgttga ttgctagtgg taatgtgctt ggcagcttcc
     1201 ttgacactgc tactaccatt tcccatagcg accgacatac caacgccagc cagcatttca
     1261 aggtcattat ctgagtcacc aaaagccatg acttggttga ggtcaaagcc atattctttc
     1321 ccaactcggc gaatgccttc taatttagaa tttccctgat tgatgacatc cgatgcaaaa
     1381 ggattgctac gtgtcaattt caagtcttca aaatcagctg ccgccttctc agattcttct
     1441 ggtgtcatca gcatcaaaac ttggtagata ggctgattca tcaggtgaag caggtcttct
     1501 tccttttggg gaacaatctt gctgaccatg cgattaaaag actgactcac cgtccgagtt
     1561 aaaacagagg gaacgaagcg actaattcgt tgagaaaaag aacccagacc aaaggacatg
     1621 attttagaac ccaacatggc atccttggtc cctagggcaa tctccgtgcc ctctttttta
     1681 gcatagctaa ttagatggcg caaatgtaac ttagaaatag ggctcgtgaa caagactctg
     1741 tctttactaa agatatactg gccattatag gttaccgcaa aatccagatc caaatcgtcc
     1801 atcaattcct taacaaaaaa aggtcctcgc cctgtcgcta cgccaactag taccccttgt
     1861 tctttgacaa tcttaatcgc gtccttagtg gatttcaaaa cactcttgcg attgttgacc
     1921 aaggttccat cgatatcaaa aaaaacagct ttgacttcca tcctatccca atctcccctt
     1981 ttgtgataca atgattatac cacatttcag aaagagtgag taaatcatgc ctaagaaaat
     2041 ccttgtttta catacgggtg gaactatttc catgcaggcc gatgcttctg gcgctgttgt
     2101 gacgagttca gataatccca tgaaccatgt gtccaaccca cttgaaggaa tccaagtcca
     2161 cgccttggac ttttttaacc ttccaagtcc ccatatcaaa cccaaacata tgctggtcct
     2221 ctaccagaaa attaaagagg aagcagataa ctacgatgga gtggtgatca cacacggaac
     2281 cgatacttta gaggaaacag cctatttcct tgataccatg gaagttcccc atatgcctat
     2341 cgttctaaca ggagccatgc gtagctccaa tgagctcggt agtgatggtg tttataatta
     2401 cctaagtgct ttacgagtgg ccagtgatga cagggctgct gacaaaggag ttttggtcgt
     2461 tatgaacgat gaaatccacg ctgccaagta tgtcaccaaa acacatacga ctaatgtcaa
     2521 caccttccag actccaacac atggccccct cggtctcatc atgaaacagg aaatcctcta
     2581 cttcaaaaca gctgaacctc gtgttcgctt tgaccttgat cacatacaag gtttagtccc
     2641 tatcatctcg gcttatgctg gtatgacaga tgagctgatt gatatgctgg atttagaaca
     2701 cttggacggt ttgattatcc aagccttcgg agctggtaat attcccaaag aaacggctca
     2761 aaaattagaa agccttctgc aaaaaggaat cccagtcgct ctggtatcac gatgctttaa
     2821 cggtattgcc gagcctgttt atgcctacca gggtgggggc gtacagttgc aaaaagcagg
     2881 cgttttcttt gttaaagaac tcaacgccca aaaagcccgc ttgaaactcc tcatcgccct
     2941 caatgccgga ctaacaggac aggctttgaa agactatatg gaaggctaat actcttcgaa
     3001 aatctctgca aaccacgtca cgtcgcctta ccgtatgtat ggttactgac ttcgtcagtt
     3061 ctatctacaa cctcaaaaac atgttttgag ctgacttcgt cagttctatc tgcaacctca
     3121 aagcagtgct ttgagctgac ttcgtcagtt ctatctacaa cctcaaaaca gtgtgttgcg
     3181 cagcctttaa ccagccgcct agtccgctct atggtattca ttaagtcaac atctcttgtt
     3241 taagagcaca aaatcaggaa atcttctcga ttccctgatt ttttctattt acgttttcgt
     3301 gttgagctac gttctgtcaa accatgaggt aagagaactt cacgttcttc caactcttcc
     3361 ttatgcataa tcttggtcaa catacgcata ctaatggcac caaggtcata aagaggttgg
     3421 gcaatcgttg tcaagtttgg acgggtaaag cgtgagattt gtgaatcatc actagtaata
     3481 atttcaaaat cttctggcac agaaacaccc ttatcagcca aaccgttcaa gactcctgct
     3541 gccaactcat cacctgtcac aactgctgca gttgcatttg atgaaatcaa acgctctgct
     3601 aaggcgtaac catcatcata gctatattta gattcaaata ccaaaccctc actataagcg
     3661 attcctgctt ttttcaaggt ttccttgtag ccaactaaac gaaccttacc attgatgtca
     3721 tccactagcg gaccgctaac gaaagcaata cgctcatttt ctttagcaag gtaactcact
     3781 gcatcaattg ttgcttgctt atagtcaata ttgacacttg gcaactggtg ctcaacatcg
     3841 acagttcctg cgagaacaat cggagtacgt gaacgcgaaa attctgagcg aattttatct
     3901 gtcaagtgat aacccatata gataatgcca tctacctgct ttgaaaagag ggtattgaca
     3961 acagaaactt ctttctcgtt atcttcatcg ctattagcta ggacaatatt gtacttgtac
     4021 atttctgcaa tatcatcaat ccccttagcc aaactcgaaa aataaccatt ggtaatattt
     4081 ggaatcacga caccgacagt ggttgtcttt ttacttgcaa gaccacgcgc aactgcattt
     4141 ggacgataat ccaagcgatc aattacctct agcacttttt tacgggtatt ctcttttaca
     4201 tttttattgc cattgaccac acggctgacc gtcgccatgg aaacacctgc ttcacgagcg
     4261 acatcataaa tggttactgt atcatctgca ttcattcctt ttcctgtcct ttctatctca
     4321 cacattcttt tacaagtaga ggtactgatt gaagctctat atctacttac aaaagtgaag
     4381 atgtgaaaat ttcgttttca tatttctact tattccattc tatcactaat tgtaaacact
     4441 ttcaagtgtt ttttgaagat tgattgaaaa aatttcatag aaaacctagg tttagctcct
     4501 tgctaccacc ttagactaaa caaaaaggag gaaactaagc cctcctaaag ttatagtaaa
     4561 atgaaataag aacaggataa atcgatcagg acagtcaaat cgatttctaa caatgtttta
     4621 gaagtagagg tgtactattc tagtttcaat ctactatagg tattgttcca ttcactaccg
     4681 tcaattttag cacatagtct tcatgaaaat attatatcat cataaccaac cagattcttt
     4741 tgcgatatta gctgcctctg ttcgattacc agcatccaat tttgaaagaa tattggtgac
     4801 atagtttcga atagttccgt ttgagaggta aagctgatct gcgatttctt ggttggataa
     4861 gccccgggcg atccccttta agacagcaat ctcttgttct gtcaacggat tgggacgggt
     4921 catcaccact tccatcaatt caggcgaata ctccttgcgt ccttcgagga cggtgtgcaa
     4981 ggtttgcatg aggtctgcaa cgcttcgttc ctttaataca taagcatcca ctccagcctt
     5041 gaccgcacgt tcaaaatacc cagcacgctt gaaggtcgtc accacaacca cctttgtttc
     5101 aagcctttct gatcgtatcc actccaagac ttcaagacct gtcttaacag gcatttctac
     5161 gtcaaggatg gcgatatcta cagactcctt ttctaatagt tggattgctt cttgcccatt
     5221 cttggcttga aggacagact ccacatctgg ttgaagcgtg agcaattgac acatggcatc
     5281 tcgcaacata ctttgatctt ctgcgactaa tactttcatc ttagtttctc tccttataag
     5341 gtagtcgaac ttgcacttcc gttggatgtt tctgactgat tacacttact tctcctgaaa
     5401 atggaaaaac acgatctcgg attgtatgga gctcatctcc ttttagaaaa gcaaagccgc
     5461 agccatcatc acttactgtt aaaatgagtt ccttctctgt ccgttctaat tttaagtaag
     5521 ctttagacgc tttggcatgt ttgatgatat tggtcaccaa ctcaagcaaa atcatagaag
     5581 ccattgactc caattcttga gtaaggctag cagtatctag ttggttatcc gtttccacct
     5641 caattccagc aatttctaac atctttttca cagtctctag ttcggatgtc aaagttctag
     5701 acttaagatt ttccacaatg gttcgaactt cacgcattga ttctttgcta atttgctgta
     5761 tttctcttaa ttccttttcc acttgtggat aagcctgcat ttgaaatagc tgcaaggcta
     5821 gatctgtctt gacactaagc atagcaaagg tatgtcccag actatcatgc aaatcctgac
     5881 cgatacgatt gcgctcattt tctgctagca atagatttat ctgggtattt tgcttgacct
     5941 gagcttcttt caaatcctcg acaaggcgaa ttcgaaccga gcccaaagtc atcaaatcga
     6001 caaaagtaag aattacaagc atatagacta gaaactcaac ttcgattctc tgaaaaatca
     6061 aaagtcgccc cacaacaagg acttgagcaa ggagaaatgt ccatacatgt aaagacttta
     6121 aactacgtac gccaaaatga taaattaaga gattggaaag gaaaaagaaa aaccagatat
     6181 aattcccagc aacaaaggcg gtattcccag ctacatagac cagcatgatc ccccagaaaa
     6241 gccatgatat gcgctggttc ttagttatta aaacacccaa atacgtcact acaaatagaa
     6301 tatcaaccaa taaatgccaa gcaggaagct ccccagtcac tacaggtagg atggggaaaa
     6361 tcataaaaat taaacttgcc caaaacatat agtgtatgct tttcagtctt tcaagcatta
     6421 agcattctcc ttatgacctt gaaggtaaat ggtcaaacca aacaaaactg ctgaaaaaac
     6481 aagtagataa actgtggctg atagattgat gccaccctcg tttaagaagg tcttgagcaa
     6541 ctccatcaat tgataggtcg ggagacactt accaactgct tgcatccagt ctggaaataa
     6601 agaaatgggc atccagagtc cgcctaaaac agccaatcct agataaagaa gattgcccac
     6661 aacagacatc agctgactag ttggtaagag agtcaaggtc aagccaagtg ctacaaaagc
     6721 aatactcccc acaatcagca aaatcgcagc tccaacccat tttactagag gcatgtctac
     6781 acctcttaca aaatgtccaa ctgtaaaaac aaccagaatt gagaccaaat aatcaaccag
     6841 catacttgtt atctttgata gataatattc taccatattt acagggctat gacgcaatgt
     6901 tttctgccag ttgttcatct tatcggtatg taaaacagct ggaaatgaga ggatagttgt
     6961 cgacatcatg gaaaaggcag tcatggagat gaggtaatca cgcataaaat tcgcgagttc
     7021 acctggtgtg tcctgataga taccagaaaa aaataaatag aaagccgtcg gcatccctac
     7081 cgacaataga taatagatta attgtcgttt ggtcaataaa aattctatct tactaagtgc
     7141 tatccatcgt ttcatcttag ttatctccct tctgcgtctc ttcaaagatt gtattcaaca
     7201 aactacgatt attaacttcg atttcttgca tcctacatcc tgcttgggct aatagttccc
     7261 agaaagcatc tgcttctcga gtgactactt gtaaagaatc ttgctttagg gtccagtttt
     7321 caaccaaatt tgactgctct acgacttcct tgtaagctat aggaaggata aagtgctttt
     7381 ctatttcctc gctacgcata gctagaggcg tcgtatctcg aatcaactct cccttattta
     7441 agagcaaaat gcggtctgca gtgtgttcca cttcctcaat ataatgggac gaatagagaa
     7501 tggtgactcc ttgcgctttt aactcctgga caatttccca aaaacgttga cgagttgagg
     7561 tatccatgga cgcagtaggc tcatctaaaa aaacaatctt tggtcgccca attaaggtca
     7621 agacgaaaga aaagagacgc ttttgaccgc ctgataattt ttctgccaac tgttcttttt
     7681 gttgcttgac aaactgcaat agttgatcaa tttcctgatt gctcaaagga tttggataga
     7741 tacgttgaaa gaaagcaatc aactctttaa cttttaattt ctgaacgatt acattttctt
     7801 gagggaggta agccctagta tagtctaact tagaactcgt cactggtaaa ccttgaatag
     7861 atacttgacc gcttgtgacc agtttatctc caagcaaaca atctaagagt gttgtctttc
     7921 cggcaccatt tggaccaatt aaggcaacac attcaccatc gttgatttca aaggagatat
     7981 tccgcaaaat ttccttgtct tttattttct tactcaattt ctcaacttta accaccgtca
     8041 tgagactctc ctttcaacca ctccattccc ataaggaaaa cgacgaaaat cataaatcca
     8101 aaccccaaag caccacgaat gaattggcga agcaaggctt gatcaaacca atcagtaaac
     8161 atttccacta accataccaa gagtgacagg ccgataaaga aatagatgat ccctctcttc
     8221 attcctcaaa ctcctttttc acatctctga ctaatttcaa accttctcta acaagccaag
     8281 acatgattcc aaagccagca aagaccttcc aaggaaaatg gtagaaatcc tcatccaatc
     8341 ccgaaaacat gagataggtc ataactcctg ctactactaa acttactgcg ataatcattt
     8401 tatttctcat ctcttcttcc cccatttcat actacaatta tagtctttta aaatcagagg
     8461 agacagaagc ttctgtcact aaaaaatatg acaaatgtca taaaaaattc tgttcaaaac
     8521 aagcaagata cactatacaa taaaacacaa tcagaaaaat ctaaggcaac ttcctcaaaa
     8581 gagatatcaa acccaattca caccataatg taaactaata cttatttaaa atcaaaaaga
     8641 gtaaaaattt ttatcagaca aacacatata tagtgtattg aatctataac agtaggcctt
     8701 aaatactaaa atatttctat aaattaattt aactttcctg atagaactgt tcatatctta
     8761 tttcaattct ctatattata cgttgaacca aacccttcta ttgctttctt aaaaatttat
     8821 aagagttata aaatctgtta aatttcaatg tgtatatcta aactacggta tttattgaaa
     8881 agactggaga caaaaagtat acgctgccaa aatgaattac tgaaaatcaa aaaagagaga
     8941 accaaactga ttccctctta atgtatataa tatctagttt taaaaataca cactcacata
     9001 tctctgt
//
LOCUS       DBERDK010000076         8973 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.076, whole genome shotgun sequence.
ACCESSION   DBERDK010000076 DBERDK010000000
VERSION     DBERDK010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8973
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.076"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(286..1134)
                     /gene="dprA"
                     /locus_tag="ACL73L_001771"
     CDS             complement(286..1134)
                     /gene="dprA"
                     /locus_tag="ACL73L_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HGS0772702.1"
                     /translation="MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDVFAIPASILDGLSDGCHHLIQEGAKLVTSGQDV
                     LAEFEF"
     gene            complement(1230..1919)
                     /locus_tag="ACL73L_001772"
     CDS             complement(1230..1919)
                     /locus_tag="ACL73L_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HGS0772703.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVRLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNMFRNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQDIIVDSKAGRILSGVSFW
                     DAPTAEKIVSFIDKAYASGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     RKLEEILKNEN"
     gene            complement(1931..2809)
                     /locus_tag="ACL73L_001773"
     CDS             complement(1931..2809)
                     /locus_tag="ACL73L_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGS0772704.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAAAHD
                     FLSIFILLAFLLVKEGKVRLSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFTAVANGQL
                     LGLMIVFAAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAVVFLGAPLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            complement(2799..3668)
                     /gene="licA"
                     /locus_tag="ACL73L_001774"
     CDS             complement(2799..3668)
                     /gene="licA"
                     /locus_tag="ACL73L_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HGS0772705.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDATSI
                     KTKFDKIVPILQTIHASGKELRGEFAPFEEIKKYESLIEEKIPYANYEAVREEVFSLE
                     KRLADLGVYRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGADFGYYGVNRYQRA
                     VKGLASYGGSDEK"
     gene            complement(3685..4707)
                     /locus_tag="ACL73L_001775"
     CDS             complement(3685..4707)
                     /locus_tag="ACL73L_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribitol-5-phosphate dehydrogenase"
                     /protein_id="HGS0772706.1"
                     /translation="MINQIYQLTKPKFINVKYQEEAIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPTGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPESEIVVIGRHWEKLELFSFAKECYITDNI
                     PEDLAFDHAFECCGGDGTGPAINDLIRYIRPQGTILMMGVSEYKVNLNTRDALEKGLI
                     LVGSSRSGRIDFENAIQMMEVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            complement(4712..5419)
                     /locus_tag="ACL73L_001776"
     CDS             complement(4712..5419)
                     /locus_tag="ACL73L_001776"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HGS0772707.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITKGGADRNTSIENIIEAIDAYRPL
                     TSEDIVVTHDSVRPFITLRMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITGIPN
                     RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
     gene            5755..7242
                     /gene="tacF"
                     /locus_tag="ACL73L_001777"
     CDS             5755..7242
                     /gene="tacF"
                     /locus_tag="ACL73L_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HGS0772708.1"
                     /translation="MKSIKLNALSYMGIRVLNIIFPILTGTYVARVLDRTDYGYFNSV
                     DTILSFFLPFATYGVYSYGLRAISNVKDNKKDLNRTFSSLFYLCIACTILTTAVYILA
                     YPLFFTDNPIVKKVYLVMGIQLIAQIFSIEWVNEALENYSFLFYKTAFIRILMLVSIF
                     LFVKNEHDIVVYTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVF
                     ANANMLFTFLDRLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGK
                     GDKEAYVSLVNRGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGGILTSLFAFRT
                     IILALDTILGSQILFTNGYEKRITVYTVFAGLLNLGLNSLLFFNRIVAPEYYLLTTML
                     SEASLLVFYIIFIHRKQLIHLGHIFSYTVRYSLFSLSFVAIYFLINFVYPVDMVINLP
                     FLINTGLIILLSAISYISLLVFTKDSIFYEFLNHVLALKNKFKKS"
     gene            7252..8055
                     /locus_tag="ACL73L_001778"
     CDS             7252..8055
                     /locus_tag="ACL73L_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HGS0772709.1"
                     /translation="MKQLTVEDAKQIELEILDYIDTLCKKHNINYIINYGTLIGAVRH
                     EGFIPWDDDIDLSMPREDYQRFINIFQKEKSKYKLLSLETDKNYFNNFIKITDSTTKI
                     IDTRNTKTYESGIFIDIFPIDRFDDPKVIDTCYKLESFKLLSFSKHKNIVYKDSLLKD
                     WIRTAFWLLLRPVSPRYFANKIEKEIQKYSRENGQYMAFIPSKFKEKEVFPSGTFDKT
                     IDLPFENLSLPAPEKFDTILTQFYGDYMTLPPEEKRFYSHEFHAYKLED"
     gene            8057..8866
                     /locus_tag="ACL73L_001779"
     CDS             8057..8866
                     /locus_tag="ACL73L_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HGS0772710.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKHDIPYFLSYGTMLGAIRH
                     KGMIPWDDDIDISLYREDYERLLKIIEEENHPRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDRFTDLSIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNTPQYEGGVGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYITPPSKEMQEWYSHSIKAYRKN"
BASE COUNT         2821 a         1816 c         1511 g         2825 t
ORIGIN      
        1 tcactatact ttcccttata ctcaatgaaa atcaaagcgc aaactaggaa gcgagccaca
       61 ggttgctcaa aatactgttt tgaggttgca gatggaagct gacgtagttt gaagagattt
      121 tcgaagagta ttatatgaga aaaatcctct actctactaa tccacttaga aaaaacgcat
      181 attatcaagt gttaaaaact tgctaatatg cgttttatta tggaaatgtt gggttcattt
      241 tctcttaaaa ttgatttttt cttagaattc tagcttaggt catttttaaa attcaaattc
      301 cgcaagaaca tcttgcccac tggtgaccaa ttttgctcct tcttgaatca aatgatggca
      361 accgtctgat agtccatcta aaatgctagc aggaatagca aagacatcgc gtccttcttc
      421 cattgctcgc tcacacgtaa tgagactacc tgaacgcatc ttagcctctg ctacaatcac
      481 accacgacaa agtccagcaa tgatgcgatt acgggcagga aaatgaaatt tcagaggttg
      541 ttcgccaggt ccatattcac ttaaaaccag atggtcattg ccgatgtagt cttgcaagcg
      601 tttattggct ttaggataaa acacatccag tcctgttcca atcactgcaa tggtttttcc
      661 gccattctga agagctgcca tatgagctgc tgtgtcaatg cccttggcca gaccactgac
      721 aataaccagt tcattttcca agccttgaat gactttttca actgatttag ctccctgttt
      781 gctacaagca cgactgccca cgaccgctac cttcgggaat ttcaggaggt caagatttcc
      841 cttgtaaaat aaaagtacag gcgcatcata tatttcactc aaatcccaag gataacagtc
      901 atctaaaata gagaaagatg gaaatttttg aaactctttc gacaaatgcg catcgtctat
      961 ctgaaaataa cgttccataa aaacggctgg attacggcaa cctgatatat ctgcaatatc
     1021 acccaacaaa agctcctgat caacattttc accgtattct agcactttca aaatctgttg
     1081 attggtcaaa cctgattttt ttaacttata gatttcatag tttgtgattt tcataaataa
     1141 ctccatttct ttttctactc atctatctat tcgtaaaagt tcgtaaaaaa tcaaaaaaca
     1201 tccgactatt ttgtcagatg ttggaatctt taattttcgt ttttaagaat ttcttctaat
     1261 ttacgatagt cttggacact atcgatctca taaatgctat tgccttctaa ttcttcaaca
     1321 tagacatcta gctctttgat attatcctta accatattgt cccagtagag atcaacaaat
     1381 tcgccacttg cataagcctt atcgataaag ctgacaatct tttctgcagt tggagcatcc
     1441 cagaaggata caccactaag gatgcgacct gccttgctat caacaataat gtcttgaacc
     1501 ttgtagtcat ctccatagac caagaaccat tcgttggtac aatcttcacg ataaacacta
     1561 aaataagtcg aacgagtcaa atcattgcgg aacatatttt taaagagata gttatctgca
     1621 tcaataacat agctgttggc caattcttct tttacaagat agagagagta aaagttattg
     1681 tagtcagcgt atttatcatt gaaaacgaga cgaacaccgt atttctcttt caagtaatcg
     1741 aattgttctt taagataacc aacaatgatg atgatgtcat tgattccttt ttctttgaga
     1801 aactcaattt ggtactcaat taagggtttt tgattaacct gaaccaaggc tttaggggta
     1861 ttttcagtca taggacgcaa gcgagttccc aatcccgctg ctaagataat ggctttcaca
     1921 cgaatctcct ttattcttta ataataatat aaactccagc aatgacgaca agtgacgtca
     1981 taatggtcag catatctagc ggtgcaccca agaaaacaac tgcaaacaag accgtccata
     2041 ctacatagct cacgttcaag cctgtagcct tggctggttg caagcgattg atagcgatat
     2101 aataagccaa gtaggaaatc atatcaaagg ctgcaaaaac aatcatgaga cctagcaatt
     2161 gtccattggc tactgcagta aatgactgat gagagaagag cacaatcaca agataggaca
     2221 agaacgaagt tacttgacgg attaagaggg cttcgatttc actcaattca ctttccatgg
     2281 caaaagagct aagaacactc tcacttcccc atgcaatagc acaaaccaaa gcacaaagaa
     2341 tcccaatgta gaaagaatta acctgttcaa ccttataggt ctgggcaata atccctccaa
     2401 taatcaagac aatcccaaat acagtatttt tcgaaatctt gtgcttcaaa aagaagaaag
     2461 ccaatagaac tgaaatcgca gggtaaatag ccgatacaga tgaagctaaa gaacttccga
     2521 tatacttaac tgcataaaga ttggcctgca taccgatagg gcctgctagc aaggctccga
     2581 tgataacact gacattgcga atatttaaga aaattgagag gcgaactttc ccttctttta
     2641 ccaagagaaa agctagtaag ataaagatgc tcaaaaaatc atgagctgca gccaccacaa
     2701 agggtgacaa atctgtaaaa atcgaaaaga tataagcact aaccgttaga cccaagcccc
     2761 agaaaatacc tgagagaagg ccaaaaggaa ctccgttttt acttttcatc tgaacctcca
     2821 taagaagcca aacctttaac agctctttgg taacgattca caccatagta accaaaatct
     2881 gcaccttgct cttccttata gacagtccat agactccaaa tagtatcttg taaaatttta
     2941 taaatagcaa tcttttcatg agaaaccggt gtttggtcac tctcatagtg agataagaaa
     3001 gtttcttcct cttgggaagt gaattcagac tctaaaaaga gggcagccaa atcccacatt
     3061 ggatcattca ttgatgaata ttcccagtca atcaaataaa gtcgtccttg aggtgattcg
     3121 ataaagtttt caggcaccaa atcgatatga caagattttc tgtaaacacc taagtcagcc
     3181 agtcttttct ctaaggagaa gacttcttct cgaacagctt catagttggc ataagggatt
     3241 ttctcttcaa ttaaggattc atattttttg atttcttcaa aaggagcaaa ttctcctctt
     3301 aattccttgc cagaagcatg aatagtttgt aatattggaa caattttatc aaatttggtc
     3361 ttaattgaag tcgcatcaag cgtaatagca gattcgatat actcatttac tttgatacca
     3421 gcttcaatat caaaaagata attttttaca tctaagccta aatcctttag tagttcaaga
     3481 ttgtactttt catcttgtcg attgataagc ttttctgtcc ctttaccaaa gaatttaaca
     3541 atgtattgct tatttgttgt tttggccaaa tagttttgat tggtcattcc acccagttgt
     3601 tcaacactga ggacttcctc ttcttgacta agtaaggaag aaattttttc tttaatgatt
     3661 ttctccacaa ttaacctcca gtacttatac ttcccactta aacactgttt taaaggctgt
     3721 gtttaaatcg gttgcaaaga cacgatgaat atctttaatt tctcttacag gttcttctag
     3781 ataaaggata tttttaagac gattggcaaa tttcttgact tccatcattt ggatagcatt
     3841 ttcaaaatca atgcgaccag aacgagatga cccaaccaaa atcaagccct tttctaaggc
     3901 atcgcgagta ttgagattga ctttatattc gctaactccc atcatgagaa tcgttccctg
     3961 aggacgaatg tagcgaatca agtcattaat agctggtcca gtaccatcac caccacaaca
     4021 ttcaaaagca tggtcaaagg ccaaatcttc aggaatatta tccgtaatat agcattcttt
     4081 ggcaaatgag aagagttcca acttttccca atgacgacca ataaccacaa tctctgattc
     4141 tggcaaagta tagttgataa tattggcaac cacaaaagct aaacttccat ctccaataac
     4201 ggcgatccgc tcccgcttgc tatgagcaag agtcaataga cgattcatag cgtgcatgcc
     4261 cacactgaca aactctgtaa tggctgcaac cgtatcttca atagcatcat aagccaccac
     4321 acgatcttta gggagagaaa caaactctct cataaagcca tcaaatccac tagacaagaa
     4381 atgggtccct gtcatgtagt tttcatagaa ttcttcatca ctctgcatag gagactgatt
     4441 gggaatcatg acaacttttt gaccaacctc gtaggttccg gtcgggtcag aaatgacggt
     4501 tccacatgac tcgtgaatca ttgccattgg aagcttttta ttcaaaatct tgggatcacg
     4561 ttttccctga tagtaacgct gatccgcatg acagacagcc atgtagttgg gacggataag
     4621 gatatgattc tcttggtcaa tagcctcttc ctgatatttg acattgataa acttaggctt
     4681 agttagttga taaatttgat taatcatttt actagtcttt ctcaatcata ctttttgcaa
     4741 tcttcaaatc tgttacggtt gtaatcttca gatttgagta ttcacctttg gccaaggcca
     4801 catcttttcc tttgatcaca aagattttac atgcatctgt caagatttcc ttctcttcat
     4861 cagaaagaga tccataaagg tccatgaagt ccttgcaacg gaatgtttga ggtgtttgtc
     4921 cctgatagag gtgagcacga tttggaatac ctgtaatgaa ttgcccattg gtactttcaa
     4981 cgatagtatc cactgcttct accactgtgt ccactgcgtc atgattttgg gcaagttgga
     5041 tattgtcctg aatcatgcga agtgtaataa atggacgaac agaatcgtgg gtaacaacga
     5101 tatcctctga agtaagcgga cgataagcat caatggcttc aatgatgttc tcaatacttg
     5161 tattgcggtc agcaccaccc tttgtaatga tgatacgttc cttataaaga ggaagatatt
     5221 tatctacaag atcttctgca tgagaaaccc agtctccatg aacaccaact acaatttttt
     5281 caatacttgg ctccaagaca aatttttcaa ttgtatgaat caaaataggt cgatcaccta
     5341 gctctaaaaa ttgttttggc aagttactga tccccatgcg tgtgccagtt ccaccggcaa
     5401 gaattcctgc ataaatcatc tattttctcc tttgtcttac taaaaaaact tattctctct
     5461 attataccac aatctagtcc tatcataaaa gaaatactgg tcctcccctt ctagaatagg
     5521 agaattatgc agttctatta ttcaaagaaa ctagtccatt tctagtaatg actaggaatt
     5581 ctgtagcaat tactaataaa tgactgcgaa attttagagt ccttcagaat aatatacttt
     5641 ccttaaagaa aacttaaaat ttaagatttg aaacttcaaa gtgattgctt ggcttactgc
     5701 ccttcatttc ttattagcta actttatgat ataatgaaaa acgaggtaaa ttgaatgaaa
     5761 agtataaaat taaatgctct atcttacatg ggaattcgtg tcttgaatat tatttttccc
     5821 atcctaactg gaacctatgt cgcgcgtgtc ttggaccgaa ctgactatgg ttacttcaac
     5881 tcagtcgaca ctattttgtc atttttcttg ccctttgcaa cttatggtgt ctatagctac
     5941 ggtttaaggg ctatcagtaa tgtcaaggat aacaaaaaag atcttaacag aaccttttct
     6001 agtctttttt atttgtgcat cgcttgtacg attttgacca ctgctgtcta tatcctagcc
     6061 tatcctctct tctttactga taatccaatc gtcaaaaagg tctaccttgt tatggggatt
     6121 caactcattg cccagatttt ttcaatcgaa tgggtcaatg aagctctgga aaattacagt
     6181 tttctctttt acaaaactgc cttcatccgt atcctgatgc tggtctctat tttcttattt
     6241 gttaaaaatg aacacgatat tgttgtctat acacttgtga tgagtttatc gacgctgatt
     6301 aactacctga ttagttattt ttggattaaa agagacatca aacttgttaa gattcaccta
     6361 agtgatttta aaccactctt tctccctctg acagccatgt tagtctttgc caatgccaat
     6421 atgctcttca cttttttaga tcgcctcttc ctcgttaaaa cagggattga tgtcaacgtt
     6481 agttactata ccatagctca gcgaattgtg accgttatag ctggggttgt aacaggtgca
     6541 attggagtga gtgtgcctcg tctcagttac tatctgggga aaggagacaa agaagcctat
     6601 gtttctctgg ttaatagagg tagtcgaatc tttaacttct ttatcattcc actgagtttt
     6661 ggactcatgg ttttaggacc aaatgccatc ctactttacg gtagtgaaaa atatatcgga
     6721 ggcggcatct tgacctctct cttcgctttt cgtacgatta tcctggcctt agataccatt
     6781 cttggttccc aaattctctt taccaatggc tatgaaaaac gtatcacagt ttatacagtc
     6841 tttgctgggc tactcaattt gggcttgaat agtctcctct ttttcaaccg tatcgtggct
     6901 cccgaatact acttactgac aactatgtta tcagaggctt ctctacttgt tttctatatc
     6961 attttcatcc atagaaaaca actcatccac ttgggacata tctttagcta tactgttcga
     7021 tactctctct tttcactttc ctttgtagca atttatttcc tgattaattt cgtgtatcct
     7081 gtggatatgg tcattaattt gccatttttg attaatactg gtttgattat cttgctatca
     7141 gctatctctt atattagtct acttgtcttc acaaaagata gcattttcta tgaattttta
     7201 aaccatgttc tagccttaaa aaataaattt aaaaaatcat aggagtttaa aatgaaacaa
     7261 ctaaccgttg aagatgccaa acaaattgaa ttagaaattt tggattatat tgatactctc
     7321 tgtaaaaagc acaatatcaa ctatattatt aactacggta ctctgattgg ggcggttcga
     7381 catgagggct ttatcccttg ggacgacgat attgatttat ccatgcctag agaagactac
     7441 caacgattta ttaacatttt tcaaaaggaa aaaagcaagt ataagctcct atccttagaa
     7501 actgataaga actactttaa caactttatc aagataaccg acagtacgac taaaattatt
     7561 gatactcgaa atacaaaaac ctatgagtct ggtatcttta tcgatatttt ccctatagat
     7621 cgctttgatg atcctaaggt cattgatact tgttataaac tggaaagctt caaactgctg
     7681 tctttcagta aacataaaaa tattgtctat aaggatagcc ttttaaaaga ttggatacga
     7741 acagcctttt ggttgctcct tcgaccggtt tctcctcgtt attttgcaaa taaaatcgag
     7801 aaagaaattc aaaaatatag tcgtgaaaat gggcagtata tggcttttat tccttcaaaa
     7861 tttaaggaaa aggaagtctt cccaagtggt acctttgata aaacaatcga tttacccttt
     7921 gagaatttaa gccttcctgc acctgaaaaa tttgatacta ttttgacaca attttatgga
     7981 gattatatga ccctaccacc agaagaaaaa cgcttctaca gtcatgaatt tcacgcttat
     8041 aaattggagg attaggatgc aatatttaga aaaaaaagaa attaaagaaa ttcaactagc
     8101 cctgctggac tatattgatg agacttgtaa gaaacatgat attccttatt ttctcagtta
     8161 tggaaccatg cttggagcca tccgccacaa aggtatgatt ccttgggatg atgatattga
     8221 tatttccctt tatcgtgagg attatgagcg tttactgaag attattgaag aagaaaatca
     8281 ccctcgctac aaggttcttt cctacgatac atcttcttgg tacttccata atttcgcatc
     8341 gattttggac acttctactg ttatagaaga ccatgttaag tacaagcgtc atgataccag
     8401 ccttttcatc gatgtcttcc caattgatcg atttacagac ttgagcattg tcgacaagag
     8461 ctataagtat gtagcccttc gtcaactagc ttatatcaaa aaatcacgag cagttcacgg
     8521 tgatagcaaa ctaaaagatt ttcttagatt atgtagctgg tacgctctcc ggtttgtcaa
     8581 tcctcgctac ttttacaaga aaattgatca actagtcaaa aatgctgtaa ccaacactcc
     8641 tcaatatgaa ggaggagttg ggatcggtaa ggaagggatg aaagaaatct tcccagttga
     8701 tacctttaaa gaactgattt taactgagtt tgagggccgt atgttgcctg ttcccaaaaa
     8761 atatgaccaa tttttaaccc agatgtatgg cgattatata acaccaccat caaaagaaat
     8821 gcaagagtgg tatagtcata gcattaaagc ttatcgcaaa aactgattga gggggattat
     8881 acaaactact aagatagagg ttattcaaaa acataatttt agtagaaaat gaaatacata
     8941 ttcccacaat aaaacgcatc atatcaaggt ttt
//
LOCUS       DBERDK010000077         8959 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.077, whole genome shotgun sequence.
ACCESSION   DBERDK010000077 DBERDK010000000
VERSION     DBERDK010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8959
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.077"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..728
                     /locus_tag="ACL73L_001780"
     CDS             <1..728
                     /locus_tag="ACL73L_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat-containing
                     protein"
                     /protein_id="HGS0772711.1"
                     /translation="IDVPAAYLEKAKGEGPFTAGVNHVIPYELFAGDGMLTRLLLKAS
                     DKAPWSDNGDAKNPALSPLGENVKTKGQYFYQVALDGNVAGKEKQALIDQFRANGTQT
                     YSATVNVYGNKDGKPDLDNIVATKKVTIKINVKETSDTANGSLSPSNSGSGVTPMNHN
                     HATGTTDSMPADTMTSSTNTMAGENMAASANKMSDTMMSEDKAMLPNTGETQTSMASI
                     GFLGLALAGLLGGLGLKNKKEEN"
     gene            895..1593
                     /locus_tag="ACL73L_001781"
     CDS             895..1593
                     /locus_tag="ACL73L_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGS0772712.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELFKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGGETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQT"
     gene            1590..2642
                     /locus_tag="ACL73L_001782"
     CDS             1590..2642
                     /locus_tag="ACL73L_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGS0772713.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVAKLEKDQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            2837..3448
                     /gene="rpsD"
                     /locus_tag="ACL73L_001783"
     CDS             2837..3448
                     /gene="rpsD"
                     /locus_tag="ACL73L_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HGS0772714.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(3542..4708)
                     /locus_tag="ACL73L_001784"
     CDS             complement(3542..4708)
                     /locus_tag="ACL73L_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGS0772715.1"
                     /translation="MQIKQITKKDGSIVYRANVYLGVDKVTGKDVKTSITGRTKKEVK
                     QKTKEAEIAFLQNGSTRFQASNITTYKELASLWWESYKHTVKPNTQLNVRRLLDNHIL
                     PLFGSYKLDKLTTPLIQNIVNKLADKTNKGEEGAFLYYDSLHALNKRILQYGVVMQAI
                     PFNSAREVILPRNTQKAKREKVKHFDNDELKSFLKYLDTLDLSRYRYYYEITLYKFLL
                     ATGCRINEALALSWSDIDLDNAIVHITKTLNYNQELNSPKSKSSYRDIDIDQATVSML
                     KQYKHRQVQEAWQLGRSETVVFSDFINEYPNNRTLQTRLRTHFKRADVPNIDFHDFRH
                     THASLLLNSGIPYKELQHRLGHSQLSMTMDIYSHLSKENAKKAVSFYETALKSL"
     gene            complement(4870..4974)
                     /locus_tag="ACL73L_001785"
                     /pseudo
     CDS             complement(4870..4974)
                     /locus_tag="ACL73L_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505376.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
     gene            4977..5159
                     /locus_tag="ACL73L_001786"
     CDS             4977..5159
                     /locus_tag="ACL73L_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000896637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772716.1"
                     /translation="MDPDKRQKIFSQCKKEGDSLEQRKQEILEKYANKQDKPKSRKND
                     SKGSESHKRKTKSKEF"
     gene            5209..6069
                     /locus_tag="ACL73L_001787"
     CDS             5209..6069
                     /locus_tag="ACL73L_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HGS0772717.1"
                     /translation="MTTNLVKQKENLEAYIRSTGYNTRGMNVENNHVLIEKPILDSYE
                     DEHQRKELVDLVNVIETRTRGGKYEVTDFESDSLQEISENFVERTESDKKKTISVDYL
                     VKLFSGKLDFSQEQLDDGQYNLTDFLGKKIIKLKRRTRNREIGKILQTAKVQTATSLD
                     DLKSIVSLINPERNVSIVISQSLFSVLEKMKDTSGNYLLKVDKETGTSETFFVDNFLI
                     VDDTTLGNEGDKKGFIGDLENFVTLFDRKKDTLSWVNANDYFRKRLILHTRFDVKKVE
                     EDCGYFIQWN"
     gene            6079..6627
                     /locus_tag="ACL73L_001788"
     CDS             6079..6627
                     /locus_tag="ACL73L_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772718.1"
                     /translation="MDINQVFDTLDDLDNKKSKIYSAREQLSEKRKSLLGIQTVSFEN
                     INSFLSNNLESLEKLEKMEKAINSLQEKYNSDFSEAKAVIFEYIFKETKQRMETKKIY
                     KQYRKKLRRILDAYDEIQELKKDVEEIHTGVVREISQKHSLSLYRTEVSPLTVLPFLN
                     PDISGWMDFSKEYRDIKEYLEK"
     gene            7005..7415
                     /locus_tag="ACL73L_001789"
     CDS             7005..7415
                     /locus_tag="ACL73L_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF722 domain-containing protein"
                     /protein_id="HGS0772719.1"
                     /translation="MYELSNRDLDGIDIELGRYRTLASKIYLRRQELIHNKKHSTEDY
                     TGGKGKTVYSPTEATIIRIEEDQTLRYLEGFKLIVDTLMENLIDTDLVIFKMRFLEAG
                     VTWEDVAEKLNKTTRYINSRRKVIAKRFIELKGY"
     gene            7791..>8959
                     /locus_tag="ACL73L_001790"
     CDS             7791..>8959
                     /locus_tag="ACL73L_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772720.1"
                     /translation="MTAEIGILNKNGVVLAADSAVTLSDGINSKVFNSARKLFTLSRV
                     HSVGIMIYGDASFMGVPWEVIISEYKKSIGNEVLADTAMYINNFVDFLLQFMPIQSND
                     ALVNYIIRQTRSYLKIIHESTQEIADHRSDQGDVITLEIFKNILWEVIEEYSNDISKS
                     ISETEFEYLDGELDLIREQLDEYFNNNEYSDEQLNFLSKTIYKAMLVGFDRNSVTGLV
                     IAGYGVEEIFPSLRQIELSGVFAKRLVWRVITEKEISQNVACHIIPFAQSEMVDTIMN
                     GIDPMLNAFIGQQINEILESNNISDAQEELFETIARVQQQYYINPIFELIGMQPIDEM
                     ASTAKTFIELTSFKRKIVNTLETVGGPVDVLAISKGEGPIWIERKHYFNIENNID"
BASE COUNT         3030 a         1418 c         1855 g         2656 t
ORIGIN      
        1 gtatcgatgt tccagcagcc tacctagaaa aggccaaggg tgaaggtcca ttcacagcag
       61 gtgtcaacca tgtgattcca tacgaactct tcgcaggtga tggtatgttg actcgtctct
      121 tgctcaaggc atctgacaag gcaccatggt cagataacgg tgacgctaaa aacccagccc
      181 tatctccact aggtgaaaac gtgaagacca aaggtcaata cttctatcaa gtagccttgg
      241 acggaaatgt agctggcaaa gaaaaacaag cgctcattga ccagttccga gcaaacggta
      301 ctcaaactta cagcgctaca gtcaatgtct atggtaacaa agacggtaaa ccagacttgg
      361 acaacatcgt agcaactaaa aaagtcacta ttaagataaa tgttaaagaa acatcagaca
      421 cagcaaatgg ttcattatca ccttctaact ctggttctgg cgtgactccg atgaatcaca
      481 atcatgctac aggtactaca gatagcatgc ctgctgacac catgacaagt tctaccaaca
      541 cgatggcagg tgaaaacatg gctgcttctg ctaacaagat gtctgatacg atgatgtcag
      601 aggataaagc tatgctacca aatactggtg agactcaaac atcaatggca agtattggtt
      661 tccttgggct tgcgcttgca ggtttactcg gtggtctagg tttgaaaaac aaaaaagaag
      721 aaaactaatc agctaaggaa ataaatgatg gatagtgggc tgactaagat tagtttaaca
      781 actcaatcag caatcaggac tttctttcaa tagcagatta aaatcatcgt aaaacaataa
      841 aaatagtgtt atacttaaag cagtatagca ctgtttttat caaaggagag acagatggga
      901 aagacaattt tactcgttga cgacgaggta gaaatcacag atattcatca gagatactta
      961 attcaggcag gttatcaggt cttggtagcc catgatggac tggaagcgct agagctgttc
     1021 aagaaaaaac cgattgattt gattatcaca gatgtcatga tgcctcggat ggatggttat
     1081 gatttaatca gtgaggttca atacttatca ccagagcagc ctttcctatt tattactgct
     1141 aagaccagtg aacaggacaa gatttacggc ctgagcttgg gagcagatga ttttattgct
     1201 aagcctttta gcccacgtga gctggttttg cgtgtccaca atattttgcg ccgccttcat
     1261 cgtgggggcg aaacagagct gatttccctt ggcaatctaa aaatgaatca tagtagtcat
     1321 gaagttcaaa taggagaaga aatgctggat ttaactgtta aatcatttga attgctgtgg
     1381 attttagcta gcaatccaga gcgagttttc tccaagacag acctctatga aaagatctgg
     1441 aaagaagact acgtggatga caccaatacc ttgaatgtgc atatccatgc tcttcgacag
     1501 gagctggcaa aatatagtag tgaccaaact cccactatta agacagtttg ggggttggga
     1561 tataagatag agaaaccgag aggacaaaca tgaaactaaa aagttatatt ttggttggat
     1621 atattatttc aaccctctta accattttgg ttgttttttg ggctgttcaa aaaatgctga
     1681 ttgcgaaagg cgagatttac tttttgcttg ggatgaccat cgttgccagc cttgtcggtg
     1741 ctgggattag tctctttctc ctattgccag tctttacgtc gttgggcaaa ctcaaggagc
     1801 atgccaagcg ggtagcggcc aaggattttc cttcaaattt ggaggttcaa ggtcctgtag
     1861 aatttcagca attagggcaa acttttaatg agatgtccca tgatttgcag gtaagctttg
     1921 attccttgga agaaagcgaa cgagaaaagg gcttgatgat tgcccagttg tcgcatgata
     1981 ttaagactcc tatcacttcg atccaagcga cggtagaagg gattttggat gggattatca
     2041 aggagtcgga gcaagctcat tatctagcaa ccattggacg ccagacggag aggctcaata
     2101 aactggttga ggagttgaat tttttgaccc taaacacagc tagaaatcag gtggaaacta
     2161 ccagtaaaga cagtattttt ctggacaagc tcttaattga gtgcatgagt gaatttcagt
     2221 ttttgattga gcaggagaga agagatgtcc acttgcaggt aatcccagag tctgcccgga
     2281 ttgagggaga ttatgctaag ctttctcgta tcttggtgaa tctggtcgat aacgctttta
     2341 aatattctgc tccaggaacc aagctggaag tggtggctaa gctggagaag gaccagcttt
     2401 caatcagtgt gaccgatgaa gggcagggta ttgccccaga ggatttggaa aatattttca
     2461 aacgccttta tcgtgtcgaa acttcgcgta acatgaagac aggtggtcat ggattaggac
     2521 ttgcgattgc gcgtgaattg gcccatcaat tgggtgggga aatcacagtc agcagccagt
     2581 acggtctagg aagtaccttt accctcgttc tcaacctctc tggtagtgaa aataaagcct
     2641 aaaacccctt tacaaatcca gctattcatg gtagaataga ttttgtgtga aatatcagca
     2701 ggaaagcatg aagctcgtca acaggtgtct tatgacaagt aaccttggct gtttaggcga
     2761 agggcatctg cacgaatcag ggctttctaa gtgactattt ccaccgaaat attatttata
     2821 tcaggaggac attcatatgt cacgttatac aggaccatct tggaaacaag ctcgtcgcct
     2881 tggcctttca cttacaggta caggtaaaga attggcacgt cgtaactacg taccaggaca
     2941 acacggacca aacaaccgtt ctaaattgtc agaatacggt ttgcaattgg ctgaaaaaca
     3001 aaaacttcgt ttcacttacg gtgtaggtga aaaacaattc cgtaacttgt tcgtacaagc
     3061 tacaaaaatc aaaggcggaa tcctaggttt caactttatg cttcttttgg aacgtcgttt
     3121 ggataacgtt gtttaccgtc ttggtctcgc gactactcgt cgtcaagctc gtcaattcgt
     3181 aaaccacggt cacatccttg ttgacgggaa acgcgttgat atcccatcat accgcgtaac
     3241 tccaggtcaa gtgatctcag ttcgtgaaaa atcattgaaa gttccagcaa tccttgaagc
     3301 agtagaagct actcttggac gtccagcatt cgtatcattc gacgctgaaa aattggaagg
     3361 ttcattgact cgcttgccag aacgcgacga aatcaaccca gaaatcaacg aagcacttgt
     3421 cgttgaattc tacaacaaaa tgctttaatt ttaagaaata tcttacagaa agcctacaac
     3481 agtgggcttt ttgctttgtc ttaaaatgtt gatttctggg tttgttcagt tatttgttca
     3541 gttataaact ttttagtgca gtttcataga acgaaactgc tttttttgca ttctcttttg
     3601 agaggtgact gtatatatcc atggtcatag ataattggga atgtcctaga cggtgctgaa
     3661 gttccttgta aggtattcca gagttaagca agagactagc gtgagtatgg cgaaaatcat
     3721 gaaaatcaat gttaggtacg tcagcacgtt taaagtgagt tcttaacctg gtttgtagag
     3781 ttcgattatt ggggtactca tttatgaagt cagaaaagac tacagtttca gagcgcccaa
     3841 gttgccaagc ttcttgtact tgtctatgtt tgtactgctt cagcatacta acagtcgctt
     3901 gatctatatc gatatcacga tagctagatt ttgatttagg actattgagt tcttgattat
     3961 agtttagtgt ttttgtgata tgcacaatag cattatctaa gtcaatatca gaccatgata
     4021 aggctaaagc ttcattgata cgacaaccag tagcaagtag aaacttataa agtgtaatct
     4081 cataataata gcgataccta cttagatcca gagtatctaa gtatttaaga aagcttttta
     4141 attcgtcatt atcaaagtgc tttacttttt ctcgctttgc tttttgtgtg ttgcgaggta
     4201 atattacctc acgagcagaa ttaaacggta tagcttgcat aactacacca tactgtaaaa
     4261 tacgcttatt taaagcgtgt aagctatcgt agtatagaaa agcgccttct tcccctttat
     4321 tggttttgtc agcaagttta ttgactatat tttgaataag tggagtagtt agtttatcca
     4381 acttataaga gccaaataaa ggtaagatat ggttatctag cagtcttcta acgtttaatt
     4441 gtgtgttagg ttttactgta tgcttgtaac tttcccacca taaggaagct aattccttgt
     4501 aagtagtaat atttgaagcc tggaatctag tagatccatt ttgtaaaaaa gcaatttcag
     4561 cttcttttgt cttttgctta acttcttttt ttgtccttcc tgttatgctt gtctttacat
     4621 cttttccagt gactttatca acaccaagat agacattagc acgatatact attgaaccgt
     4681 ctttttttgt gatttgtttt atttgcatag tattcttttc catcagcagg caagcaatta
     4741 gaaaaggttt tgagtttata ccatgctagg agctacgaga acccctctat tttcgatttt
     4801 aagagaacga acggtaaaat gtaccaggta agaaaatgaa gcgattatgg aacttataaa
     4861 gggtattaat tagataatct tctcaaatac cattgtggct tgaatgcggt caccaccgcc
     4921 taaacctttg cttccaccat tggcggttgt gatggtatgc aggtgataag ttttctatgg
     4981 atccagataa aagacagaaa atcttttctc agtgtaaaaa agaaggtgat agcttagaac
     5041 aacggaaaca agaaatacta gagaaatatg ctaacaaaca agacaaacca aaatctagaa
     5101 aaaatgattc taaaggctcg gagagtcata aaagaaaaac taagagcaaa gaattttaga
     5161 aaaaattata aacaaaaatc agatattaaa agatgaagga gcaaaaaaat gacaactaac
     5221 ttagttaaac aaaaagaaaa tttagaagct tatatccgaa gtacaggtta taacactaga
     5281 gggatgaatg tagaaaataa tcatgtactc attgaaaaac caatccttga tagttacgaa
     5341 gatgaacatc aacgtaaaga actggttgat ctagtaaatg ttattgagac tcgtacccgt
     5401 ggcgggaagt atgaggtaac tgactttgaa tctgattcat tacaagaaat tagtgaaaat
     5461 tttgttgaga gaacagaatc agataaaaag aaaactatca gcgttgatta cttagttaaa
     5521 ttattcagtg gaaaacttga tttttcacag gaacaattag atgatggcca atataattta
     5581 acggattttc ttggtaagaa gattattaaa ttaaaacgta gaacacgaaa tagagagatt
     5641 gggaaaattc tccaaactgc gaaagtacag accgctacaa gtctggacga cttgaaatct
     5701 attgtttctt taatcaatcc agagcgcaat gtatctatag ttattagtca atcactattt
     5761 agtgtcttag aaaaaatgaa agacacttca ggaaattatc ttcttaaagt tgataaagag
     5821 acaggaacga gtgaaacatt ctttgtagat aactttttaa ttgtagatga tacgacatta
     5881 gggaacgaag gcgacaaaaa aggatttatc ggagatctag aaaactttgt tactttgttt
     5941 gatcgaaaga aagatacact tagttgggtg aatgcgaatg actattttag aaaacggttg
     6001 attttacata cccgatttga tgtaaaaaaa gttgaagaag attgtggtta ctttattcaa
     6061 tggaactagg aggaagaaat ggatattaat caagtatttg atacactgga tgatttagat
     6121 aataaaaaaa gtaagattta ttcagcacga gaacagttaa gcgaaaaaag gaagagtctt
     6181 ttaggcattc aaacagtttc atttgagaat ataaattctt ttttgtcaaa taacttagaa
     6241 tctttagaaa agctggaaaa gatggaaaaa gctattaatt ctcttcagga aaaatataat
     6301 agtgattttt cagaagctaa agcagtcatc tttgaataca tttttaaaga aactaagcaa
     6361 cggatggaaa ctaagaagat ctataaacaa taccgaaaga aacttagacg aattctggac
     6421 gcatatgatg aaattcaaga actaaagaag gatgtagaag aaattcatac aggtgtagtc
     6481 agggaaataa gtcagaaaca ttctctatcg ctatatcgaa cggaagtaag tccgcttact
     6541 gtcctaccat tcttaaaccc tgatattagc gggtggatgg atttttctaa ggaatatcgg
     6601 gacattaaag agtatttaga aaaataggga acaaattaag taaggctagt gatatatggc
     6661 tgaaacagaa gagatatcgc tagtcctact tttatgcttt actaagtttc acataacaaa
     6721 gtaagcataa actaaaatag agtataggct tggaagtcat atatatcagt aagttacaga
     6781 atggagtgag tttcacagaa tgtaagatat gagaaactta ggggataaat taaagaaatt
     6841 tcccttgaac ttgtcatatt gaagagttgt caaacttaaa acaatgatac ctggtaagtg
     6901 gagtgttgga aggcttttag cgctttttgt cagtttgaca gaatttacaa tttgacaaat
     6961 tgaaagataa aaaaattttt aaatttaagt ggaggtactt gcctatgtac gagttgagta
     7021 acagagacct ggacgggata gatattgagt taggacgata tagaacgctt gctagtaaaa
     7081 tttatttaag aagacaggaa ctgatacata ataagaaaca tagcactgaa gattatactg
     7141 gtggaaaagg caagacagta tatagtccta ccgaagcaac tattattaga attgaagaag
     7201 accaaacact aagatattta gaaggtttca aactaattgt agataccttg atggaaaatc
     7261 tgattgatac tgatctagtc atctttaaaa tgagattttt agaagctggt gtgacttggg
     7321 aagatgtggc agagaaacta aataaaacta ctcgttatat aaatagtcga agaaaagtaa
     7381 tcgctaaaag atttatagaa ctgaaaggat attgactccc cccacgttga aaaaaatttt
     7441 ttgaatactt tgagaaccgg tgaagggaac tttttctaag tcggagacct ccagacaaaa
     7501 agggggtaaa aacttgttga tttataaggt gaaggctagt tttttgaaat taaacatatg
     7561 cattcaaaag tcattgaaat aaatgaggag gttaaactga agataatttt tctagattaa
     7621 agtgaagcta tgatatattg ttagaaattg gagatgtgtt tgcatagtta aaagaatgta
     7681 cttttagtat gaattgtatt ataattagtt aaagggaatt gttgtataaa ttgaaaaata
     7741 tttaatgatt tttaactgtt aagttatgta aaataattgg aggctcaatt ttgactgcag
     7801 aaattggaat actaaataag aatggagttg ttttagctgc agatagcgcg gtgactttat
     7861 ctgacgggat taactccaaa gtgtttaata gtgctcggaa attatttact ttatctagag
     7921 tgcattcggt tggaattatg atttatggag atgcctcctt tatgggtgta ccgtgggaag
     7981 taatcataag tgagtataaa aagtcaattg gcaacgaagt actagctgac acagcaatgt
     8041 acattaataa ttttgttgat tttttattgc aatttatgcc aatacaaagt aatgatgcct
     8101 tggttaatta tataattaga cagactagaa gttatttaaa aataatacac gagagcaccc
     8161 aagaaatagc tgatcataga tcagatcagg gagatgtaat aactctagaa atttttaaaa
     8221 atattttatg ggaagttata gaagaatatt caaatgatat ctccaaatca atatctgaaa
     8281 ctgaatttga atatttagat ggcgaacttg atttaatacg ggaacaacta gatgagtact
     8341 ttaacaataa tgaatactcc gatgagcaac taaacttttt atctaaaaca atatacaaag
     8401 caatgttagt tggttttgat agaaattctg taacagggtt ggtaattgct gggtatggtg
     8461 ttgaagaaat ttttccatcc cttagacaaa tagagttaag tggtgtattt gctaaacgcc
     8521 ttgtctggcg tgtaattacc gagaaagaaa ttagccaaaa tgttgcttgt catattattc
     8581 cctttgctca atccgaaatg gtagatacta taatgaatgg catagatcca atgttaaacg
     8641 catttatagg acaacaaatt aatgaaattt tggaaagtaa taatatatcg gatgcacagg
     8701 aagagttgtt tgaaactatc gctagagtcc aacaacaata ttatataaat ccaatttttg
     8761 agttgattgg gatgcaacct attgatgaga tggcttctac tgcaaaaaca ttcattgagt
     8821 tgacttcttt taagagaaag attgtgaata cacttgaaac agttggagga ccagtggatg
     8881 ttttagctat ttctaaaggg gaaggtccta tctggattga aagaaaacac tactttaata
     8941 ttgaaaataa tattgatta
//
LOCUS       DBERDK010000078         8710 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.078, whole genome shotgun sequence.
ACCESSION   DBERDK010000078 DBERDK010000000
VERSION     DBERDK010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8710
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.078"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(394..1590)
                     /gene="tuf"
                     /locus_tag="ACL73L_001791"
     CDS             complement(394..1590)
                     /gene="tuf"
                     /locus_tag="ACL73L_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HGS0772721.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
     gene            complement(2512..3381)
                     /gene="gla"
                     /locus_tag="ACL73L_001792"
     CDS             complement(2512..3381)
                     /gene="gla"
                     /locus_tag="ACL73L_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaglyceroporin Gla"
                     /protein_id="HGS0772722.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTENPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQFMKQKAIEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIAA
                     AIAAVAVFKFLYL"
     gene            complement(3605..4126)
                     /locus_tag="ACL73L_001793"
                     /pseudo
     CDS             complement(3605..4126)
                     /locus_tag="ACL73L_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862277.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(4084..5190)
                     /locus_tag="ACL73L_001794"
     CDS             complement(4084..5190)
                     /locus_tag="ACL73L_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HGS0772723.1"
                     /translation="MNIILIAKLLRENTNTKANALNNGWARSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVGSGDSKLGKGGYFSTGDVLLGKDVVSYTVQVFSENNE
                     RVGVNTQSHRVQYNLPILADFSVIQDTVEPSRTVVEKIIPKLNIPEEEKGKITEEIKK
                     KKKTSELAELISENVKVRYVDEQGRLLSLKNDTGIGEKESDGTYITNKKQLIGTSYNV
                     TDKKLSSMTTTDGKYYTFKEVDTNSASLTGNIVSEGRTVTLVYRESEAPTTVTVTANY
                     YKEGSQEKLAESVIKADLAIGSEYTTESKTIEGKTTTEDKEDRVITRKTTYTLVATPA
                     NAYQKTVQQLTITTVRMLRKQWFPKQQPLLRRRL"
     gene            complement(5301..5555)
                     /locus_tag="ACL73L_001795"
     CDS             complement(5301..5555)
                     /locus_tag="ACL73L_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772724.1"
                     /translation="MLNVDQDFMSISKSNKSGSDWKKTFTVRTTNRLANDLNNVLKQA
                     DKDTPNTPTWLNSAASKAKDDDRAYKLLKTLIPGENYLSC"
     gene            complement(5549..5791)
                     /locus_tag="ACL73L_001796"
     CDS             complement(5549..5791)
                     /locus_tag="ACL73L_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772725.1"
                     /translation="MVQNEDLYKKGLNVELAHQQIKGFFEAEFKNRINGVLNTKIKNS
                     TLNRVNKKTIHQSNKNSMINLKQKQRKMLKNKAILC"
     gene            complement(5895..6739)
                     /locus_tag="ACL73L_001797"
                     /pseudo
     CDS             complement(5895..6739)
                     /locus_tag="ACL73L_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078372828.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            6973..8691
                     /locus_tag="ACL73L_001798"
     CDS             6973..8691
                     /locus_tag="ACL73L_001798"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HGS0772726.1"
                     /translation="MSYQENYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGT
                     AGMRGLVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFE
                     SAAVLAKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDG
                     GQMPPHDADALTTYIRAIENPFAVEVADVETEKASGLIEVIGEAVDIEYLKEVKDINI
                     NPALIEEFGKDMKIVYTPLHGTGEMLARRALAQAGFDSVQVVEAQATADPDFSTVTSP
                     NPESQAAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAK
                     YILEAHKNAGTLPENAALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMMGFEESFGYLIKPFVRDKDAIQAVLVVAELAAYYRSRGLTLADGIEEIYKE
                     YGYYAEKTISVTLSGVDGAEQIKAIMAKFRNNAPTEWNATAITVVEDFKAQTATVADG
                     TVTNLTTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKIANIEAE
                     INAFVK"
BASE COUNT         2483 a         1786 c         1637 g         2804 t
ORIGIN      
        1 ttgatttcat attactattg tataatgctt tttgatttta gtctagtatt atactcttcg
       61 aaaatctctt caaaccgcgt cagcttcgcc ttgtcgtata gatgttactg acttcgtcag
      121 ttctatctac aacctaaaaa cagtgttttg agcagtctgt agctaacttt ctatagtaaa
      181 atgaaataag aacaagacaa atcgatcagg acagtcaaat cgatttctaa caatgtttta
      241 gaagtagatg tgtactattc tagtttcaat atattatagt ttgctctttg attttcattg
      301 agtattagtt caatatctaa aaaaagaacc ttgccaagca agattctttt agtgtctgac
      361 ttaaataatt gttcttctgg gaactaaatc gaattaagct tcgatttctg taaccatacc
      421 tgaaccaaca gtacgtccac cctcacggat agagaatgta gtaccttgtt ctacggcgat
      481 tgggtgaatc aactcaacgt cgattgtcac gttatcacca ggcattacca tttcagtacc
      541 tgctggaagt tcgattgaac ctgtaacgtc agtagtacgg aagtagaatt gtggacggta
      601 gttgttgaag aatggagtgt gacgtccacc ttcttcttta gtaaggatgt agacttcacc
      661 tttgaattta gtgtgtgggt tgattgaacc tggtttagcg ataacttgtc cacgttcgat
      721 ttcatcacgt tgaacaccac gaagaaggac acctacgtta tctccagcaa gaccttcgtc
      781 aagttgttta cggaacattt caacaccagt aacaactgct ttttgagttt cttctttgat
      841 accaacgatt tcgatttcgt cgttgacttt aacgatacca cggtcgatac gtcctgaagc
      901 aactgtacca cgtccagtga ttgagaatac gtcctcgact ggaagaagca atggtttgtc
      961 agtgtcacgt tctggttctg ggatatactc atcaactgtg ttcatcaatt ccataacgat
     1021 gtcttcgtat ttagagtcac cttcaagagc tttaagtgct gaaccttgga taactggaag
     1081 atcgtcacct gggaagtcgt attctgacaa taggtcacgg atttccattt caaccaattc
     1141 aagcaattct tcgtcgtcaa ccaagtcaac tttgttcatg aagacgataa ggtgtttaac
     1201 accaacctga cgtgaaagaa ggatgtgctc acgagtttgt ggcattggtc cgtcagttga
     1261 agctactaca aggatagctc cgtccatttg agcagcacca gtgatcatgt ttttaacgta
     1321 gtccgcgtgt cctggagcgt cgatgtgagc gtagtgacgt ttttcagttt cgtactcaac
     1381 gtgcgcagtg ttgatagtga taccgcgttc gcgttcttct ggagcagcat cgatagacgc
     1441 atagtcttta ggttggttaa ctgatgaagg caagcgacgt gccaaaacag ttgtgatagc
     1501 tgcagttagg gtagttttac cgtggtcaac gtgtccgata gtaccaatgt taacgtgtgg
     1561 tttactacga tcgtattttt cttttgccat ttgagtaaaa gcctccaata aaatatattt
     1621 tatagataga cagtaggcaa tacagtctaa ctttccttac tattttatca aatttcagct
     1681 aaattgcaag tgttttacaa cgtttttgtg aattattctt ggtaaaaatt taactaatta
     1741 atagaaagta ctaaatagac ttatccttca aggagttttc agcattatcc atccactcac
     1801 ttgctgaaaa tgtaaacagt ctctggttta tatagtaaaa tgaaacaaga atagggcaaa
     1861 tcgatcagga cagtcaaatc gatttctaac aatgttttag aagtagatgt gtactattct
     1921 agtttcaatc tactatacaa cctcaaaact gtgttttgaa caacctgcgg ctagcttctt
     1981 agtttgccct ttgattttca ttgagtataa aattgcagat aaaacgataa caatcatagg
     2041 actcagcaga gtcaatgtag gtcgaagaat agccccccta atcgcttatt ttatagcaaa
     2101 atgaaataga aactgaacat tttgttgagg aaggtaaatc aattcctcac aatgtttgag
     2161 aaagaaggga ggactattcc aacttcaacc cactataaag cagacttaca agatagggaa
     2221 cccatctccg ataatatata gtagaatgaa aactgcacaa tttgattgga gaattcaaac
     2281 caatttctaa caatgtttta gaaacaaggg tctattattt caactgcaac ctactatagc
     2341 ccattgaagc tggaatagcc cactgagact tctaaaccac tgctggagag gagtttgttt
     2401 agagtttgtt tagagtttct ttcatttccc taaaaaatca aaaagagaga accagtctga
     2461 tcctctctta cttatagatg gtgctcactc aaatgttaaa ggagctattt cttagagata
     2521 aaggaatttg aatacagcta ctgccgcaat tgctgctgcg ataggtgcta ctactggtac
     2581 ccaagaatac caccattttg aatcgccttt atgctcacca agaactgatt ttggaaggaa
     2641 agcatgaagg agacgtggtc ccaagtcacg ggctgggttc aaggcaggtc ctgtaggtcc
     2701 tccaagtgat gttaccaaag ccataacgag gaatccaagt gccaagtgag ccacagaaag
     2761 tcctgaagca gtgtgtggag ccacctgtgc tttaatagcc aagtcagaaa aatcaactgt
     2821 ttgtcctgct tctattgcct tttgtttcat gaattgaagc acttcagcac caaagaagtt
     2881 tttagtcaaa ccaagagctg caaagaacaa aacaaatgaa ccaacaaact cattaatcaa
     2941 accattgaca gttgctgcat agcgactttc ttttgtacca tggtcaatac ttgaaatagt
     3001 tgagaaagtt cccaagatgt tatttgggtt ttcagttttc aagtagaatg gacggtatgt
     3061 tgccacaact aaggcttggc caaagatagc ccccaagact tgcgcgataa tgtaaggtac
     3121 cacttgtgcc caagggaaaa gaccgctaac tgcaagccct agagtgaaag cagggttgat
     3181 gtgattccca gatacgttac caaacatcaa ggctgggatc ataaccccca taccataacc
     3241 aacagcgatg acgatccagc cactttggtg acctttcgta cctttaagtt caacgttggc
     3301 aactgcacca ttcccaagaa tgatcaaaat ggcagttccc aaaaattcag tggcatactt
     3361 cagtgcccat gtgaaatcca tttgctagat tctccttaaa attttttaga tattccttat
     3421 tttatcaatt tttaccctat ttagcaactg atttctggaa atatttttaa aaaaatagga
     3481 ttcatagttt cccagttcaa cggacttgta agcgttttta ttcttcacga aagacctcgt
     3541 tctcaaaacc aggaaaaaag cccacatctg tgagcttttc cttgttccta gattctaaaa
     3601 tctattattt ttcatcttct tttttcttga agaatcctaa tcctagacca gctaacaagg
     3661 tcatgatacc agcactagcc aaggtagcat tagcttctgt acctgtgttt ggcaattcct
     3721 ctctcttacc tgtctcataa gtcggaactt ctgggtctgg attcactgga gtttcagttt
     3781 ttggagtacc tggttctgga gttggtttat ctggtgttga taaacggtca taccttaccg
     3841 ttatttcttt atcactagag tctgacgtaa cttcttgtga ttaaactgtt ggaatatctg
     3901 gagctttgta cttgtcaatc ttaccagata taacctcgtc ccagtttcct gttgtccatt
     3961 caccgtaggt tacaactccc gtgaccttgt tctcagtttt tgtacggctt aaggttacag
     4021 gttgaacaac atcttctttt acattttggt tcgtaacttt atcaacgtaa tgaatgatac
     4081 gcgttatagt cttcgtctca gtagaggttg ctgttttggg aaccactgtt tcctcaacat
     4141 tctcacggta gtaatagtca actgttgcac cgtcttctgg tacgcatttg caggagttgc
     4201 taccaaggtg tatgttgttt tccttgtgat aactcggtct tctttgtcct cagttgttgt
     4261 tttcccttca atagtttttg attctgtggt atactcagaa cctatcgcta aatcagcttt
     4321 tataacagac tctgccaact tctcttggct accttcttta tagtaattgg ctgttactgt
     4381 aacagtggtt ggcgcttcgc tttctctata aactaaggtc actgttctac cttcgcttac
     4441 aatattccca gttaaacttg cagaatttgt atctacttct ttaaaagtat aatattttcc
     4501 gtcagtagta gtcatgctac tgagtttttt atctgtgaca ttgtagctgg taccaatcag
     4561 ttgtttttta ttggtaatgt aggttccgtc actttctttt tctccaattc cagtatcatt
     4621 tttcaatgat agcaaacgcc cttgttcatc aacatagcga actttcacat tttctgagat
     4681 tagttctgcc aattctgaag tttttttctt tttcttgatt tcttcggtta ttttcccttt
     4741 ctcttcttcg ggaatattta gttttggaat gattttttca acaacggttc gtgatggttc
     4801 cacagtatct tggatgactg aaaagtcagc tagaattggg agattatact gaacacggtg
     4861 actttgagtg tttactccta ctctttcatt attctctgaa aatacttgta cggtataaga
     4921 aacaacatct tttcctaata gaacatcccc agtagagaaa tagccgcctt ttcctagttt
     4981 gctatctcca gagcccactt ctttcctaat cttatcagat agttttttac cagtcagtac
     5041 attcgttcgc acaatccctt tgtctacccc tacaaagtgg gagaactttt tgaactcttc
     5101 agaaccagat ctagcccaac cattattaag ggcatttgct tttgtatttg tattctctct
     5161 caaaagtttg gcgattagaa ttatattcat cggcacttag agttgctgct atatctgact
     5221 cttgaatacc aacttcctta ctaccatttc tagcggcagt atatgtgtat ttatctgttt
     5281 ctacttctag ctgattatcc ttaacatgat aggtaatttt ctcctggtat aagagtcttc
     5341 agtagtttat atgctctgtc atcatcttta gctttagaag cagctgagtt tagccaagtt
     5401 ggggtattag gagtatcttt atcagcctgt ttcaagacat tattcaagtc atttgctagc
     5461 ctattggttg tcctcactgt gaaagttttc ttccagtctg aaccactttt attagactta
     5521 gatatgctca tgaaatcctg gtcaacattc aacacaatat cgccttgttt tttagcatct
     5581 tccgttgctt ctgcttcaaa ttgatcatgg agtttttgtt gctctggtgt atagtttttt
     5641 tatttacacg atttaatgta ctatttttta ttttagtatt aagaactcca ttaatacgat
     5701 ttttaaactc tgcttcaaaa aatcccttaa tttgttggtg cgcaagttca acattcaaac
     5761 cttttttata caagtcttca ttctgaacga tttcggctac acggtgaatg gtttgattgt
     5821 ataccgtctt ctcttaacca gcagtatcct tagttttaat gactactcga taggttcctt
     5881 tagaaccgtt atagtcatct agttttaaaa aagcattcca aacattcaaa aatagttgga
     5941 acttccctta ataaatccgt cactttcttt ttcaagatag cccaagcttg ctcaatagga
     6001 ttcaaatccg gtgaataagg tggtagaggt aagaaaaagt gtttatcttg gatgcagagt
     6061 tcatccaaaa tgttcttggg atgaaaacca gcattgccca tgacaataac atgaggtgtc
     6121 ttcaaagcag gtaggagttg cgttttgaac cactccacaa agaaatcgct tgtcatgctt
     6181 ttcttgtaaa tcatgggagc tataaactct ccgtctactt gtcctgcaac aattgaagtt
     6241 cgctcaaaac gacgtccgct aatcttttca tagactttct cccctctagg agcccctgca
     6301 taaggacgat agagatagcg gtcgattcct gtttcatcaa tataaacagg tgctaggtgc
     6361 tttaaactat taaaattctt aagaaataag gctacttttc tgggtcttgt tccttaaagc
     6421 tcgtcatctt tttttaaagt gaccttaatc tgctttaaag ctgcccatac tgaaggaaca
     6481 gcacaatcaa aatgtgccgc aatttcccgt aaaaaagcat ctggatgagc ctctacaaag
     6541 gctttcaatt cctctaaagg aatctttcgg tttttgacga ctcgcttttt cctctctatg
     6601 tgttcttgtt cacgtctttc ttttcccacg tgaagagagt tctgacgcca acaccaaaaa
     6661 acttggctgc ctcgacatgg ctgtgcccct ctttgatgta atctaatgct cgctgtttaa
     6721 aatcagtact atatgccata gctttattat aatatgcttg tttaaaacta agtaactata
     6781 tcaatgtttt gttatgagaa gacttacttt ttttactgaa attgaatttt tcctagcctc
     6841 aacatcatat aaccccaaaa ttcaatctga cctggtttga agagattttc gaagcagata
     6901 aggttttact aaaactaaaa aaaatgatat aatcaaactg attaaatgtt ttaaaaataa
     6961 aggagaattt gaatgtctta ccaagaaaat taccagaaat gggttgattt tgtggagctt
     7021 cctgactacc ttcgtcaaga tttggaaaat atggacgaaa aaactaagga agatgccttc
     7081 tatacaaatc ttgaatttgg tactgcaggt atgcgtggct tggttggtgc tggtacaaac
     7141 cgtatcaaca tctacgttgt tcgccaagct actgaaggat tggctcgttt gattgagtca
     7201 aaaggtggaa acgagaaaga acgcggtgta gcaattgcct acgatagccg tcacttctca
     7261 cctgagtttg cctttgaatc tgcggcagtt cttgctaaac acggcatcaa atcttacgta
     7321 tttgaaagcc ttcgtccaac tccagaacta tcatttgcag ttcgtcatct caactgtttc
     7381 gcaggtatca tggtcacagc cagccacaac cctgctccat ttaacggtta caaggtttac
     7441 ggtgaagacg gtggacaaat gcctccacac gatgcggacg ctttgactac ttatatccgt
     7501 gcaatcgaaa acccatttgc agttgaagtt gctgatgtgg aaactgaaaa agcttctggc
     7561 ttgattgaag ttatcggcga agctgttgac atagaatacc ttaaagaggt taaggacata
     7621 aacatcaacc cagccttgat tgaagaattt ggtaaagaca tgaagattgt ctacacacca
     7681 cttcatggta ctggtgaaat gttggctcgt cgtgctcttg cccaagcagg atttgactct
     7741 gttcaagttg ttgaagcgca agcaactgct gacccagact tctcaactgt aacatctcca
     7801 aacccagaaa gccaagcagc ctttgccctt gctgaagaac ttggtcgtca agttggtgca
     7861 gatgttcttg tcgcaactga cccagacgct gaccgtgttg gtgttgaagt tcttcaaaaa
     7921 gatggtagct acctcaacct ttcaggtaac caaatcggtg ctatcatggc taaatacatc
     7981 ttggaagctc acaaaaacgc tggaactctt cctgaaaatg ccgctctctg caaatctatc
     8041 gtttcaactg acttggtaac gaagattgct gaaagctacg gcgcaactat gttcaacgtc
     8101 ttgacaggtt tcaaatttat cgctgaaaaa atccaagaat tcgaagaaaa acacaaccac
     8161 acttacatga tgggatttga agaaagcttc ggttacttga ttaaaccatt tgtacgtgat
     8221 aaagatgcca tccaagccgt tcttgtcgtt gctgaacttg ctgcctacta ccgttctcgt
     8281 ggtttgacac ttgctgacgg tatcgaagaa atctataaag aatatggcta ctacgcagaa
     8341 aaaacaatct ctgttactct ttcaggtgtc gatggtgctg aacaaatcaa agcgattatg
     8401 gctaaattcc gcaacaatgc tccaacagaa tggaacgcaa cagctatcac tgtcgtagaa
     8461 gacttcaagg cacaaactgc tactgttgct gacggtactg ttacaaactt gacaactcct
     8521 ccaagtgatg tgttgaaata cacacttgct gacggttcat ggattgccgt tcgcccttca
     8581 ggtacagaac caaaaatcaa gttctacatt gcagttgtag gggaaaccaa cgaagaatca
     8641 caagctaaga ttgctaacat cgaagcagaa atcaatgcat ttgtaaaata aagacctata
     8701 aaagatggct
//
LOCUS       DBERDK010000079         8665 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.079, whole genome shotgun sequence.
ACCESSION   DBERDK010000079 DBERDK010000000
VERSION     DBERDK010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8665
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.079"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(8..1172)
                     /locus_tag="ACL73L_001799"
                     /pseudo
     CDS             complement(8..1172)
                     /locus_tag="ACL73L_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251597.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1269..2120)
                     /gene="rfbD"
                     /locus_tag="ACL73L_001800"
     CDS             complement(1269..2120)
                     /gene="rfbD"
                     /locus_tag="ACL73L_001800"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HGS0772727.1"
                     /translation="MILITGANGQLGTELRYLLDERNEEYVAVDVAEMDITNEEMVEK
                     VFEEVKPTLVYHCAAYTAVDAAEDEGKELDFAINVTGTKNVAKASEKHGATLVYISTD
                     YVFDGKKPVGQEWEVDDRPDPQTEYGRTKRMGEELVEKHVSNFYIIRTAWVFGNYGKN
                     FVFTMQNLAKTHKTLTVVNDQYGRPTWTRTLAEFMTYLAENRKEFGYYHLSNDATEDT
                     TWYDFAVEILKDTDVEVKPVDSSQFPAKAKRPLNSTMSLAKAKATGFVIPTWQDALQE
                     FYKQEVR"
     gene            complement(2186..3235)
                     /gene="rfbB"
                     /locus_tag="ACL73L_001801"
     CDS             complement(2186..3235)
                     /gene="rfbB"
                     /locus_tag="ACL73L_001801"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HGS0772728.1"
                     /translation="MTEYKNIIVTGGAGFIGSNFVHYVYENFPDVHVTVLDKLTYAGN
                     RANIEEILGNRVELVVGDIADAELVDKLAAQADAIVHYAAESHNDNSLNDPSPFIHTN
                     FIGTYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGHGEGPGEKFTAETKYNPSSP
                     YSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILSGIKPKLY
                     GEGKNVRDWIHTNDHSSGVWTILTKGQIGETYLIGADGEKNNKEVLELILKEMGQATD
                     AYDHVTDRAGHDLRYAIDASKLRDELGWKPEFTNFEAGLKATIKWYTDNQEWWKAEKE
                     AVEANYAKTQEIITV"
     gene            complement(3245..3841)
                     /locus_tag="ACL73L_001802"
     CDS             complement(3245..3841)
                     /locus_tag="ACL73L_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HGS0772729.1"
                     /translation="MTDNFFGKTLAARKVEAIPGMLEFDIPVHGDNRGWFKENFQKEK
                     MLPLGFPESFFAEGKLQNNVSFSRKNVLRGLHAEPWDKYISVADGGKVLGSWVDLREG
                     ETFGNTYQTVIDASKGIFVPRGVANGFQVLSDTVSYSYLVNDYWALELKPKYAFVNYA
                     DPSLGIEWENIAEAEVSEADKHHPLLKDVKPLKKEDLE"
     gene            complement(3842..4711)
                     /gene="rfbA"
                     /locus_tag="ACL73L_001803"
     CDS             complement(3842..4711)
                     /gene="rfbA"
                     /locus_tag="ACL73L_001803"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HGS0772730.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPVYDKPMIYYPLSTLMLA
                     GIRDILIISTPQDLHRFQELLQDGSEFGLKLSYAEQPSPDGLAQAFIIGEEFISDDSV
                     ALILGDNIYHGSGLSKMLQKAASKESGATVFGYHVKDPERFGVVEFDQDMKAISIEEK
                     PEQPRSNYAVTGLYFYDNDVVEIAKSIKPSPRGELEITDVNKAYLDRGDLSVELMGRG
                     FAWLDTGTHESLLEASQYIETVQRMQNVQVANLEEIAYRMGYISREDVLALAQPLKKN
                     EYGQYLLRLIGEA"
     gene            complement(4868..5260)
                     /gene="tagD"
                     /locus_tag="ACL73L_001804"
     CDS             complement(4868..5260)
                     /gene="tagD"
                     /locus_tag="ACL73L_001804"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047348 - glycerol-3-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HGS0772731.1"
                     /translation="MKRVITYGTFDLLHHGHINLLRRAKELGDYLVVVVSSDEFNLIE
                     KNKVCYFNYEHRKSLVEAIRYVDLVIPETSWEQKRSDVKEYHIDTFVMGDDWIGEFDY
                     LKEEGVEVVYLPRTKEISTTKIKKDLSM"
     gene            complement(5288..6103)
                     /locus_tag="ACL73L_001805"
     CDS             complement(5288..6103)
                     /locus_tag="ACL73L_001805"
                     /inference="COORDINATES: protein motif:HMM:NF016852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HGS0772732.1"
                     /translation="MTETNKQLHETLIEILDFVKEICEKHELTYFLVFGTALGAKRHC
                     GFIPWDDDVDIALPREHYNIFIDALSETDQSIFSLQNEDNEPNYFLPFAKLRKNNTIF
                     IEKILDVEYENNGIYIDIFPLDFVENPDSFNFKIRRTTFNYIKHILKFSSCRSFYKNK
                     YSNVRYLIENIMSIPTLFFSNRRLLFLANSLISSTTKADFIGQYDQKSNKRAIMPSNY
                     YFPPRSAVFEGKTYSVPAKLEDYLKCFYGSDYMELPPIEKRVTHQPITLRFEK"
     gene            complement(6113..7552)
                     /locus_tag="ACL73L_001806"
     CDS             complement(6113..7552)
                     /locus_tag="ACL73L_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HGS0772733.1"
                     /translation="MKKILNKYYSLSNPVKASIWFTICNVLQKGISMITVPVFTRVLT
                     TEQYGVYSVYQSWYSIIGVFATLNLYYGVFNNGMIKYEKDKNVFTSSMQGLTTTVTAI
                     FLLIYLIGIDFWNSLLGLPTLLILVMFFDLFFTPAYSFWVARQRFEYKYRNLVFITFI
                     IAIGSPIIGISAVVLSTYKAEARVISFVLVQSCIGLYFYILNLYRGKHFFCKKYWLYA
                     LNFNLPLIPHYLSQSVLNQSDRIMINSMVGMGEAAIYSVAYSISILMLLVTSAINSSF
                     IPYTYKCIRDKKYTELGKSANLLITLVGIGSILTISLGPEIIQLFAPKQYYEAIWIIP
                     PVALSVYFMFLYPIFGNIEFYFEANHFVMWASIGGAIMNIFLNFIFLKHFGYIAAGYT
                     TLFCYILFALGHYIFMRRVLYQNLSGIQIYNSRYLLFFSILLIILMLLIVIIYPFILI
                     RYLTISLILGGCFLKRKKIMSSIAIIKQQ"
     gene            complement(7570..>8665)
                     /locus_tag="ACL73L_001807"
     CDS             complement(7570..>8665)
                     /locus_tag="ACL73L_001807"
                     /inference="COORDINATES: protein motif:HMM:NF016357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HGS0772734.1"
                     /translation="NKLLIFGSWLGEKYADNPRYLFEYVIKNRPDLKAIWITSNQDVF
                     KELQKKQYLVMMAEAPETRKIVKKAKYIFTATGIFDIGEQNANFVGGAYLINLWHGIP
                     LKKIMYDDKHSALHKRSKLVTWVEKIPLRNYFVISTSTAITQIYQSAFRVKKSNILEL
                     GQPRNDYFYDKSNPVSSLIQELKSKNIILYMPTHRNEGKKQIDLDKLMDLEHLNNWCE
                     ETKSIFVIKKHFYHSKEKTIDKKYSSIIDVTNEKVDVQELLKCSNVLITDYSSCYIDY
                     LLLNRPIIFFNYDYDDYLRVDRSLYFPYENVTPGEKCQNFDELLVTLQNLYVGKDDYR
                     EERENIKTFFYSSETQKSVSEKIINHVLNL"
BASE COUNT         2724 a         1758 c         1369 g         2814 t
ORIGIN      
        1 agatttctta tttgcacttt tcttgtacaa ctttagtcca tggtaaataa gcctctaaaa
       61 cctctttgtt tacgagagtt tcctcgtttg gaagacattc tagaagatag aatagatatt
      121 tctctctatt taattgatga cgtttagctg tttccaacaa actcataata atagctcttg
      181 ctttagctcc ttcaaaactt tgagaaaata accaccctcg cttttttaaa ctgagcttag
      241 gctaaaagag tccactggac tcttttactc cgtcccataa ccaatgattt aatggagcat
      301 tcagctagat tattggaaag gaccagatgt ccgtctttca aaatagtctt aaaggtttct
      361 tcatacttga ggctgtattc aattgccctt cctagttttg aacctgctaa aactgactga
      421 cggcggcacc aagcaaagaa gtcttccttt aagggttgga gctcttcttg acgtttctgt
      481 agtcgttcat cagctggcaa agcctcccag tctctttcca aggaaaataa ctgattacaa
      541 taagctaaac ctttagctcc taaggatgat ttatccgctt gcttgggggt cgcttcaaaa
      601 aacttccttc tcacatgtgc ccaacatcca acaagtgcag cttcttccag ttgacgataa
      661 gcttctagcg gttcgcagtt taccaagctt agcgtcgcct tgctcctaaa ccttggactg
      721 ctatcgcata ggcagaacta gaggactaaa gtcctaagtt actgccgcaa catatcacaa
      781 tgcacatagc cagaataatc tcctaggaat tcttgtacta ctaaaccact tcgacactga
      841 ccatgatggt acagcgtgat tgcttgattc tcagctttcc cagacaaaaa ggtccaatag
      901 taggtcagat ggctatcact ctctagaacc cgataagagg tttcatccgc aggaagaaga
      961 ggttgttcta acaacttttc tcgtaaaagc ttataaaggg actctaaata gtattgactt
     1021 gccttgatat gccaattaga gatttcctta cgtgtgattg gtaaacccat cctagcccaa
     1081 tcttcttctt ggcgataatt gggtaccttc agattaaact tctgatggat ggtgtgagcg
     1141 ataatagaaa ctgagccaaa gctatgcgcc aaagagacca gcaccaacaa caagataagc
     1201 gtacatctta agctcctttg gagttcatta tctgcctctt ttattagact agaaaatcat
     1261 tctactactt atctcacttc ttgtttgtaa aattcttgca atgcatcttg ccaagttgga
     1321 ataacaaatc cagtagcttt ggctttggcc aggctcatcg ttgagtttag cggacgttta
     1381 gctttggctg gaaattgact ggaatctact ggcttgactt cgacatctgt atctttcaaa
     1441 atttcaactg caaaatcata ccatgttgtg tcttctgtcg catcatttga caaatgataa
     1501 taaccaaatt ccttacgatt ttcagctagg taggtcatga actcagccaa ggtacgagtc
     1561 caagtcggac gaccgtactg gtcatttaca actgttaaag tcttatgagt tttcgcaaga
     1621 ttttgcatgg taaaaacgaa gtttttgcca taatttccaa atacccaggc agtacggata
     1681 atatagaaat tagacacatg cttctcaact aactcttccc ccatacgctt agtgcgtcca
     1741 tattctgtct gtggatctgg tcggtcatca acttcccact cttgtccaac tggtttctta
     1801 ccgtcaaaga catagtccgt agaaatataa actagagttg caccatgctt ttcagatgct
     1861 tttgcgacat tttttgtccc cgtcacattg atggcgaagt ccaactcttt tccttcatcc
     1921 tctgctgcat caacagcggt gtaggctgca caatggtaga ctaaagtcgg tttcacctct
     1981 tcaaaaactt tctcaaccat ttcttcattg gtaatgtcca tctcagccac atctactgcc
     2041 acgtattctt cattacgttc atccaataaa tagcgaagtt ccgttcctaa ttggccattt
     2101 gcccctgtaa ttaaaatcat ctctagacct ttctaatatg taactcttcc caatatagca
     2161 ataaaaagca gctatttcct gctttttata ctgtaataat ctcctgagtc ttagcataat
     2221 tggcttcaac agcttctttt tctgctttcc accattcttg gttatctgta taccacttga
     2281 ttgttgcctt gagcccagct tcaaagttgg taaattcagg tttccacccc aactcatcac
     2341 ggagcttgct ggcatcaatc gcatagcgaa ggtcatgtcc tgcacggtca gtcacatgat
     2401 cataggcatc cgtagcttgt cccatttcct taaggataag ttccaaaact tccttattgt
     2461 tcttctcacc atcagcccca atcaagtagg tttcaccgat ttgccctttt gtcaagattg
     2521 tccaaactcc tgaagaatgg tcattggtat gaatccagtc acgaacattc ttaccttcac
     2581 cgtaaagttt tggcttaatt ccacttagga tgttagtaat ctgacgtggg atgaattttt
     2641 cgatatgttg ataaggaccg tagttatttg aacagttgga aatcgttgcc ttgactccaa
     2701 aagaacgcac ccaggctttg acaatcaaat ctgaggctgc cttggttgat gagtacggag
     2761 agcttgggtt gtattttgtt tcagcagtaa atttctcacc tggtccttca ccatgacctg
     2821 gcaaatcttc gcgtaaaggg agatccccat aaacttcatc tgtcgataca tggtggaagc
     2881 gaatatcata cttacgagca gcttctaaaa gagtataggt tccgatgaag ttagtatgaa
     2941 taaatggcga tggatcattg agcgaattat cattgtggct ttccgctgca taatgaacga
     3001 tagcatctgc ttgagcagcc aacttgtcta ccaactccgc atcagcaatg tcaccaacaa
     3061 ctaactcaac acgattacct aaaatttcct caatattcgc gcggtttcca gcataagtca
     3121 acttatctag gactgtcaca tgaacatctg gaaagttctc gtaaacataa tggacaaagt
     3181 tagaaccgat aaagccagct ccacctgtca cgataatatt tttgtattca gtcatattct
     3241 ttccttattc caaatcttct tttttcaaag gttttacatc cttaagtagt ggatgatgtt
     3301 tgtctgcttc tgaaacctct gcttctgcaa tattttccca ttcaatacca aggcttggat
     3361 cagcgtagtt cacaaaggca tacttgggtt tgagttcaag agcccagtaa tcattgacca
     3421 gatagctata tgacactgta tctgataaaa cttggaagcc attagctacg cctcgaggaa
     3481 caaagattcc cttactcgca tcaatcactg tctgataggt attcccaaag gtttcaccct
     3541 cgcgtagatc aacccaagaa cccagaactt tccctccatc tgctacagag atgtacttat
     3601 cccaaggctc tgcgtggaga cctcgaagga catttttacg tgagaaggat acattgtttt
     3661 gcaattttcc ttctgcaaag aaagactctg gaaatccaag tggaagcatt ttttcctttt
     3721 ggaaattttc tttaaaccag ccacgattat ctccatgaac ggggatatca aactccaaca
     3781 tgcctggaat agcttcaacc ttgcgtgccg caagcgtctt accgaaaaaa ttatctgtca
     3841 tctatgcttc tccaatcaaa cggagcagat actgtccgta ttcatttttc ttaagtggtt
     3901 gggctaaggc caatacatct tctcgactga tatagcccat acggtaagca atttcttcta
     3961 agtttgctac ctgaacattt tgcatccgtt gcactgtttc gatgtactgt gaagcctcta
     4021 gtaaactttc atgagttcca gtatccagcc aagcaaagcc acgtcccata agctcaacag
     4081 acaaatcacc acgatctagg taagccttgt ttacatctgt aatttccagt tcaccacgag
     4141 gacttggttt aatactcttg gcaatctcta ctacatcatt atcatagaaa tagagacctg
     4201 taactgcata gtttgaacga ggttgctctg gcttttcttc aatagaaata gccttcatat
     4261 cctgatcaaa ctcaaccaca ccaaagcgct ctggatcctt gacatggtag ccaaaaacag
     4321 ttgctcccga ctccttactc gctgcctttt gtagcatctt ggaaagccca gaaccgtgat
     4381 agatattgtc acctaagatc agcgcaacgc tatcatcaga aataaactct tccccaatga
     4441 taaaggcttg tgccaaacca tctggacttg gttgctctgc ataagaaagt ttgagcccaa
     4501 actcagatcc gtcttgaaga agctcttgga atcgatgtaa atcctgtgga gtggaaataa
     4561 tcaaaatatc cctaatccca gccaacatca atgttgaaag tgggtagtaa atcatcggtt
     4621 tatcataaac cggcataagt tgttttgatg cagcgcgagt caaaggatat aaacgtgtcc
     4681 ccgaaccacc tgctagaata atacctttca tagtagggta cctttcttat ttatctaatt
     4741 acgatatttt ttacctatat tggtagtgtc ctatcatttt tctcttttct atcgatttta
     4801 gcatttctac ttaaaaatac agtggtctat tcgagaacaa taacagaaca atgcttattt
     4861 acgaatatta cattgataaa tcttttttta ttttagttgt cgaaatttct ttcgtccgag
     4921 gtaagtaaac gacttcaact ccttcttctt ttagataatc aaattctcca atccaatcat
     4981 cccccatcac aaaagtatca atatgatact ctttgacatc actgcgtttt tgttcccaac
     5041 tcgtttcagg aataacaagg tcaacatatc taatagcttc aactagactt tttctatgct
     5101 cataattgaa ataacaaact ttattttttt caattaggtt gaattcatca cttgaaacaa
     5161 ctaccactag atagtcacct aattcttttg cacgtcgcaa taaattgata tgtccatgat
     5221 gaagtaaatc aaacgttcca tatgtaatta ctcttttcat tatattctcc ttctacagaa
     5281 tagcaaatta tttttcgaat ctaagtgtta ttggttgatg agtgactctt ttttctattg
     5341 gtggtaattc catataatcc gagccataaa aacattttaa ataatcttct aatttcgcag
     5401 gaacactata ggtctttcct tcaaacacag cggacctagg tggaaaataa tagtttgaag
     5461 gcataatagc tcttttgtta cttttctgat catattgacc aataaaatca gctttagtcg
     5521 ttgatgaaat taaactattc gctaagaata ataatctcct atttgaaaaa aacagggtag
     5581 gaatactcat aatattttca atcaaataac gcacattact gtatttattt ttataaaaac
     5641 ttctgcaact tgaaaatttt aaaatatgct ttatataatt aaatgtagtt ctcctaatct
     5701 taaaattgaa agagtccgga ttctctacga aatctagtgg aaaaatatca atgtaaatgc
     5761 cgttattctc atattcaaca tcaagaatct tctcaataaa aatcgtattg ttttttctta
     5821 atttagcaaa tggtaaaaaa taattcggct cgttatcttc attttgaaga ctaaatatag
     5881 attgatccgt ctcagataat gcatcgataa atatattgta gtgctctcgt ggcaaagcaa
     5941 tatcaacatc atcatcccat ggaataaatc cacaatggcg ttttgctcca agagctgtcc
     6001 caaagaccaa aaaataagtt aattcatgtt tttcacaaat ttcttttaca aagtctaaaa
     6061 tttcgattaa tgtttcatgt aactgtttat ttgtttctgt cattcttgta cctcactgtt
     6121 gtttaatgat agctatcgaa ctcattatct ttttcctctt taaaaaacat cctcctagga
     6181 tgagtgagat agtaagataa cgaatcagta taaatggata aataatcacg attagtagca
     6241 ttagaataat aagaagaatc gaaaaaaata aaagatatct ggaattataa atttggatac
     6301 ccgaaagatt ctgatacagt actctcctca taaaaatgta atgtcccaac gcaaataaaa
     6361 tataacagaa taaagttgta tagcctgccg caatatatcc aaagtgtttt aaaaaaataa
     6421 aattcaaaaa aatattcata attgctccac cgattgaagc ccacattaca aaatgatttg
     6481 cttcaaagta aaactcaatg ttaccaaaaa tgggatataa gaacatgaaa tatactgaca
     6541 gagcaacagg aggaataatc catattgctt cgtaatattg ttttggtgca aataattgaa
     6601 tgatttctgg tccaagagaa atagttaaaa tcgaaccaat gcctaccaga gtaattaaaa
     6661 gatttgcact tttccctagt tcagtatatt tcttatctcg aatacactta tatgtgtaag
     6721 gaataaaaga gctgtttata gcacttgtca ctaaaagcat caaaattgat atgctgtaag
     6781 caacactata tattgctgct tctcccattc ctaccattga attaatcatt attctatccg
     6841 attggtttaa aactgattgc gataaataat gaggaataag aggtaaatta aaattcaaag
     6901 catagagcca atatttttta caaaaaaaat gtttacctcg gtaaaggttc aaaatataga
     6961 aatataaccc tatacaagat tgaactaaca caaatgagat aactctagcc tccgctttat
     7021 aagtcgataa aacaactgca gaaataccaa taataggact tccaatcgct ataataaatg
     7081 tgataaatac taagtttcta tatttatatt caaatctctg acgagcaacc caaaaagaat
     7141 aagcaggggt aaaaaacaaa tcaaagaaca tcactaagat tagtagtgta ggtaagccta
     7201 acaacgagtt ccaaaaatca atccctatta aatatatcag taaaaatata gctgtaactg
     7261 ttgttgttag tccctgcata gaggaagtaa agacattttt atctttctcg tatttaatca
     7321 ttccattatt aaagactcca taatataggt ttagtgttgc aaaaactcca attattgaat
     7381 accatgattg atatacagag tatacaccat attgttccgt tgtcaatact ctagtaaaaa
     7441 ctggaacagt aatcattgaa attccttttt gaagaacatt gcaaatagta aaccaaattg
     7501 aagcttttac aggattagat aaagaataat acttatttaa tatttttttc atgacagatt
     7561 ccaataaagt tatagattta gtacgtgatt aattattttt tcagaaactg acttctgagt
     7621 ttcactagaa taaaagaaag ttttgatatt ttcacgttct tctctataat catcttttcc
     7681 tacatataga ttttgcaaag tcactaataa ttcatcaaag ttttgacatt tctcccctgg
     7741 agtaacattc tcatatggaa aatatagcga acgatccacc cgcaagtaat catcataatc
     7801 ataattaaaa aatataattg gacgattcaa caataagtag tcaatatagc agcttgagta
     7861 gtcagttatt aaaacatttg aacatttgag taattcttga acatccacct tctcatttgt
     7921 cacgtcaatt atcgaagaat atttcttatc gatagttttt tcttttgaat gatagaaatg
     7981 ctttttaatt acaaaaattg actttgtttc ttcacaccaa ttatttaagt gttctaaatc
     8041 cattaactta tctagatcta tttgtttctt tccttcattg cgatgtgtcg gcatatataa
     8101 gataatattt ttacttttta attcctgtat taatgaactc actggattag acttatcata
     8161 aaaatagtca ttcctaggtt gaccaagttc taatatatta gatttcttta cgcgaaaagc
     8221 agactgataa atttgagtta ttgccgtact agtagaaata acaaaatagt ttcgaagagg
     8281 aattttttct acccaagtca ctagtttact acgtttgtga agtgctgaat gtttatcatc
     8341 atacatgatt tttttcaaag gaatgccatg ccatagatta attagatagg cacctccaac
     8401 gaagttagca ttttgttctc caatatcaaa aataccagtt gctgtaaaaa tatactttgc
     8461 ttttttaaca attttccgag tttcaggtgc ctctgccatc atcacgagat attgtttctt
     8521 ctgtaattct ttaaaaacat cttgattact tgtaatccat atagctttta aatcgggacg
     8581 attcttaatc acatactcaa acaaatatct tggattgtct gcatattttt cacctaacca
     8641 tgatccaaaa attaacaatt tattt
//
LOCUS       DBERDK010000080         8494 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.080, whole genome shotgun sequence.
ACCESSION   DBERDK010000080 DBERDK010000000
VERSION     DBERDK010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.080"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..450
                     /locus_tag="ACL73L_001808"
     CDS             <1..450
                     /locus_tag="ACL73L_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate uptake regulator PhoU"
                     /protein_id="HGS0772735.1"
                     /translation="ICLQHPVSYDLRRIISVIKISTDIERIGDRIVEILKNLQILQNN
                     EILKKIISEIKILHEVIGLHMNRAISCYREEQSGCLDMVVIQKQNEIEELSTNIEKKI
                     MNYIFEDDGNVSEVIGALDIIHHLDKIAHTTQSIYKWIMYRKYGNIN"
     gene            463..1938
                     /gene="galT"
                     /locus_tag="ACL73L_001809"
     CDS             463..1938
                     /gene="galT"
                     /locus_tag="ACL73L_001809"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HGS0772736.1"
                     /translation="MKTIIDNFVDRVIEYGMYNEIDKIYVKNRVLALIGEEGTDRISD
                     ENDLKQIKDYLVEIALKNGKIKDLIEEKECLGAELMNFIVPLPSRLNDIFWSSYDISP
                     QEAVEEFYKLSKDSDYIKTSAIAKNIEFRASTKYGELEITINLSKPEKDPKTIAAEKL
                     VKATNYPKCLLCMENEGYQGRINYPARSNHRIIRLKLGDEVWGFQYSPYLYFNEHAIF
                     LNSQHVPMAITSKTFEQLLEIVDILPGYFAGSNSDLPISGGSILSHNHYQGGKHIFPM
                     EKAKFESEFRFKDFEDVNAGIVKWPMSVIRLQSENKNRLLDLATKILNKWREYSDLEV
                     DIIAMTEDVPHHTVTPIARKVDGRYELDIVLRDNHTTEQYPDGVFHPHQDVQHIKKEN
                     IGLIEVMGLAILPPRLKPELEEVGKYLLGEDNAIADYHLEWADQLKEKYPRINKEEVN
                     SVVQHEAGQVFARVLEDAGVYKNTPSGHEAFMRFVKSVGIN"
     gene            1951..2930
                     /gene="galE"
                     /locus_tag="ACL73L_001810"
                     /pseudo
     CDS             1951..2930
                     /gene="galE"
                     /locus_tag="ACL73L_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996558.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
     gene            3140..3799
                     /locus_tag="ACL73L_001811"
     CDS             3140..3799
                     /locus_tag="ACL73L_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HGS0772737.1"
                     /translation="MKNLTKIKFKENGEFNHFPGNTVVANLYTKQDLMEVVDIIQSRY
                     RELPFIDKFTLTPRNSIHMTVIELLCHENRETEFWSSNLPLDTPLQEIHDYFAKQLEI
                     FPLLDEEIHMRITEMGKQNILVEPADEASAKRLEEIRTYVSEKAGVCFPNHDRYQFHI
                     SIGYLRIPLTEEEEEEFTKVRAELTEILLEKIPTITVNRIDYTVFEDMRQFVPYHEKF
                     K"
     gene            3824..4891
                     /locus_tag="ACL73L_001812"
     CDS             3824..4891
                     /locus_tag="ACL73L_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HGS0772738.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIAGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDVKEMPKDWT
                     ELGSKYKGKYSIYGLQGGTGRAILASILVRYLDEKGDLGVSEKGWEVAKEYFANAYTL
                     QKGESSVVKALDKESPIQYGMIWGSGALIGQKEQNVEFKVMTPEIGVPFVTEQTMVLS
                     TSKKQALAKEFIDWFGQSEIQVEYSKKFGSIPANKDALKELPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWVEKAELEYVK"
     gene            4994..6004
                     /locus_tag="ACL73L_001813"
     CDS             4994..6004
                     /locus_tag="ACL73L_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0772739.1"
                     /translation="MIKFDNIQIKYGDFVAIDNLNLDIHEGEFFTFLGPSGCGKSTTL
                     RALVGFLDPSSGSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VAPDVIKAKVSAVAAKIKISDQQLQRNVSELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVDLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPV
                     EIYHNSQTEFVCDFIGDINVLTDETVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETEQ
                     DFILKGTIIDVEFSGVTIHYTIKVSESQILNVTSIDSQAAIRSVGESVELFITPSDVL
                     QF"
     gene            6016..7707
                     /locus_tag="ACL73L_001814"
     CDS             6016..7707
                     /locus_tag="ACL73L_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS0772740.1"
                     /translation="MRHKLNLKDWLIRLGLIWFLVTFIIYPNFDLVVNVFVKGGEFSL
                     DAVHRVLKSQRALQSIMNSFKLAFSLIITVNVVGILCVLFTEYFDIKGAKILKLGYMT
                     SLIYGGVVLATGYKFVYGPYGLITKFLQNVIPSLDPNWFIGYGAVLFIMTFSGTANHT
                     LFLTNTIRSVDYHTIEAARNMGAKPFTVFRKVVLPTLIPTLFALTIMVFLSGLSAVAA
                     PMIVGGKEFQTINPMIITFAGMGNSRDLAALLAIILGIATTILLTIMNKIEKGGNYIS
                     ISKTKAPLKKQKIASKPWNIIAHIVAYGLFTVFMLPLIFIVLYSFTDPVAIQTGNLTL
                     SNFTLENYRLFFSNSAAFSPFLVSFIYSIIAATTATILAVVFARVVRKHKSRFDFLFE
                     YGALLPWLLPSTLLAVSLLFTFNQPQFLVLNQILVGSLVILLIAYIVVKIPFSYRMVR
                     AILFSVDDEMEDAARSMGASPFYTMMKVIIPFILPVVLSVIALNFNSLLTDFDLSVFL
                     YHPLAQPLGITIRSAGDETATSNAQALVFVYTIVLMIISGTVLYFTQRPGRKVRK"
     gene            7710..8420
                     /locus_tag="ACL73L_001815"
     CDS             7710..8420
                     /locus_tag="ACL73L_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HGS0772741.1"
                     /translation="MTATSLGLSNIEIVVRIVLSVVIGSIIGLERGSKSQPAGIRTYS
                     IVCLAACLIMMTNEYVSYKFGTGDPTRLGAQVISGVGFLGAGTILITDKKKITGLTTA
                     AGIWASAGIGLAIGVGFYEGALLVAISVWGVISMFQPLKKYLQNRSKMIELYIVVKST
                     EAYNRVLVYCAENGIRMTDSRTAFGDVNSDRIEYFDVPDKKIASFITLELSGRFEHLR
                     LMEEIANIVGVIYVEEIS"
BASE COUNT         2900 a         1173 c         1660 g         2761 t
ORIGIN      
        1 atttgtttac aacatcccgt ttcatatgac ttaagaagaa ttattagtgt aataaaaata
       61 tcaactgaca ttgaacgtat tggggataga attgttgaaa ttttaaaaaa tctacagatt
      121 cttcaaaata atgaaatttt gaagaaaatt atttcagaaa taaaaatact tcatgaagtt
      181 atcggactgc atatgaatag ggctatatct tgttatagag aggaacaatc tggatgttta
      241 gatatggttg ttatacaaaa acagaatgag atagaagagc ttagtacaaa tattgaaaag
      301 aaaataatga attacatatt tgaagatgat gggaatgttt ctgaagtaat tggtgcttta
      361 gatattattc atcaccttga taaaattgct catactacgc aatcaattta taaatggata
      421 atgtatagaa aatatgggaa cataaattag aaagaaaaat atatgaaaac aattattgat
      481 aattttgttg atagagtaat tgaatacgga atgtataatg aaattgacaa aatatatgtg
      541 aaaaatagag tactagcgtt aataggtgag gagggaactg atcgcatttc cgatgaaaat
      601 gacctgaaac aaataaaaga ttacttagtg gaaatcgcct taaaaaacgg taaaattaaa
      661 gatttaatcg aggaaaagga atgtttagga gcagagttaa tgaatttcat agttccttta
      721 cctagtcgat taaatgatat tttttggagt tcatatgata tatcacctca agaagcagtt
      781 gaagagtttt ataagttaag taaagatagt gattatatta aaacttctgc tattgctaag
      841 aatattgagt ttcgagcatc aactaaatac ggagagctag agattacgat aaatttgtca
      901 aaacccgaaa aagatccaaa aacaattgct gctgaaaaat tagtaaaagc cactaattat
      961 cccaaatgtt tactatgtat ggaaaatgaa ggatatcagg ggcgaataaa ttatccagca
     1021 cgttcgaatc atagaattat tcgattgaaa cttggtgacg aagtatgggg attccaatat
     1081 tctccctact tatactttaa tgagcatgcg atatttttaa atagtcaaca tgtaccaatg
     1141 gctattacct ctaaaacatt tgaacaatta ttggagattg ttgatatttt gccaggatat
     1201 tttgctggtt caaattctga tcttcctatc tcaggaggct ccattcttag tcataaccac
     1261 taccagggag ggaaacatat tttcccaatg gaaaaagcaa aatttgagag tgaatttcgt
     1321 tttaaagact ttgaggatgt taatgctggt attgtaaaat ggccaatgtc agtaattagg
     1381 ttacaaagtg aaaataaaaa tcgcttgtta gatttagcta ctaaaattct aaataagtgg
     1441 agagaatatt cagatttaga agtagacatc attgctatga ctgaagatgt cccacaccat
     1501 acggtgactc ctattgctcg taaagtggac gggagatatg aattagatat tgttctaaga
     1561 gataatcata ctactgaaca gtacccagat ggtgttttcc atcctcatca agatgtacaa
     1621 catattaaaa aggaaaatat cgggttaatc gaagtgatgg gacttgcgat attaccacct
     1681 cgtttgaaac cagagttaga agaagttgga aaatatcttt taggagagga taacgcaatt
     1741 gcggattatc atttagaatg ggccgaccaa ttgaaagaaa aatatcctcg cattaataaa
     1801 gaagaggtta atagcgtagt tcaacacgaa gcaggacaag tgtttgcgag agttctggaa
     1861 gatgcaggag tttataagaa tacaccgtct gggcatgaag cgtttatgag atttgttaag
     1921 tcggttggaa ttaattagga ggaaacaaaa atggcaatat tggtaacagg cggagctggt
     1981 tatattggta gccataccgt agtagaatta ctaaatttag gaaaggaagt catcattgtc
     2041 gacaaccttt cgaactctag catcttggtg ctagaccgta ttgaagcaat tacaggaata
     2101 cgtcccgtgt tttacgaatt agatgtttgt gataaaccag cattgagaaa ggtatttgaa
     2161 caagaatcga ttgatgctgc aattcatttt gcaggttata aagctgtcgg agaatccgtg
     2221 caaaagcctg tgatgtacta caaaataata ttatgagtac attggcactt gttgaagtga
     2281 tgtcagaatt taatgttaaa aagattgtct tttcttcaag tgcgactgta tatggaatta
     2341 acaatcagtc acctctaatt gagactatgc aaacaagtgc gacaaatcct tatgggtata
     2401 cgaaagtgat gcttgagcaa attttaaaag atgttcatgt ggcagattca gaatggagta
     2461 ttgcgttgct tcgttatttc aatccaattg gtgctcatga gtctggtttg attggggaag
     2521 atccctcagg aattcctaac aacttgatgc cctatattgc acaagtagcg gtaggtaagc
     2581 tgtcagaact aagtgtgttc ggaaatgatt atgatacgct tgacggtact ggtgtgcgcg
     2641 attatatcca tgtagtagat ttagcaatag gacatataaa agctttagaa aaagtatctg
     2701 aaaaaacaga tgtttatatc tataacctag gctcgggaga aggcactagt gtattacaac
     2761 ttgtaaatac atttgaaagt gttaataaga tccctattcc ttataaaata gttccaagac
     2821 gttcaggaga cgttgcaact tgttatgcaa atgcagacaa agcatataag gaattaaatt
     2881 ggaggacaac aaaatcgatt gaagacatgt gtagagatac atggaattag caatcaaaaa
     2941 atcccaatgg ctataattga tgatactgtc tatatgagtg ctgtattttg gaaaaattac
     3001 ttaacctttt aatgaaatgc atatttaatg aaagataaat taaaaaaaat gtttgacagc
     3061 gcttgacaaa ggaaacggtt tcattgtata atgtaaacat agtgacaccg atgtcaaata
     3121 taaaaaagaa ggtctttata tgaaaaactt aacaaaaatt aagttcaaag agaatgggga
     3181 atttaatcat ttccctggga atacagttgt agcaaatctt tatactaaac aagatttgat
     3241 ggaagttgtt gatattattc aatcacgtta tagagaatta ccatttatcg ataagtttac
     3301 tttaactcca aggaattcta ttcacatgac tgtaattgaa ttgttgtgcc atgaaaatcg
     3361 tgaaacggag ttttggagca gtaatcttcc tctagataca cctttacagg aaatacatga
     3421 ttactttgct aaacaacttg aaatttttcc attgttggat gaagaaattc atatgcgtat
     3481 aactgaaatg ggaaaacaaa acatactagt tgaacctgca gatgaagctt ctgcaaagag
     3541 attagaagaa attcgtactt atgtctcaga aaaagcaggt gtttgtttcc ctaatcatga
     3601 tagatatcaa ttccatattt caattgggta tcttcggatt cctctaaccg aagaggagga
     3661 agaagagttt actaaagtca gagcagaatt aactgaaatt ttattagaga agattccaac
     3721 tattactgta aaccgtattg actatactgt atttgaagat atgagacaat ttgttccata
     3781 tcacgaaaaa tttaaataaa ataataaaaa aggagaactt atcatgaaaa aaatgaaagt
     3841 ttggtctact gtacttgcaa cgggagttgc tcttactaca cttgctgctt gctctggagg
     3901 ttcaaattct acgactgctt cttcatctga agaaaaagct gataaaagtc aagaattagt
     3961 aatctattcg aactcagtct caaatggtcg tggtgattgg ttaactgcta aagcaaaaga
     4021 agctggtttt aatataaaaa tggttgatat tgctggcgct caattagcag accgtgttat
     4081 tgctgagaag aataatgcag ttgcagatat ggtatttgga attggtgctg ttgactcaaa
     4141 taaaattaga gatcaaaaat tactagtaca gtacaagcct aaatggttag ataaaattga
     4201 tcaatcttta tcagataaag ataattatta taatcctgtg attgttcaac cattagtttt
     4261 aattggggcg cctgatgtaa aagaaatgcc taaagattgg actgaattag gtagtaagta
     4321 taaaggtaaa tattcaattt atggtcttca aggtggtaca ggacgggcaa ttctagcaag
     4381 tatcttagtt cgttaccttg atgaaaaagg agatttaggc gtatcagaaa aaggatggga
     4441 agttgcaaaa gaatactttg caaatgccta tactcttcaa aaaggtgaaa gctcagttgt
     4501 taaagcgtta gataaagaaa gcccaattca atacggtatg atctggggtt ctggagcgtt
     4561 aatcggacaa aaagaacaaa atgttgaatt caaagttatg actcctgaga ttggtgtacc
     4621 atttgtaact gaacaaacta tggttttaag cactagtaaa aaacaagcgt tagctaaaga
     4681 atttattgat tggtttggtc aatcagaaat tcaagtagaa tatagtaaaa aatttggatc
     4741 tattcctgca aataaagatg ccctaaaaga gttacctgaa gatacgaaaa aatttgttga
     4801 tcaagtgaaa ccacaaaata ttgactggga agctgttgga aagcatttgg atgaatgggt
     4861 agaaaaagct gaattagaat acgtaaaata agaaataaga gttatttttt atgtccaagt
     4921 ctctctatta tttctaaatt taaggaagaa ataaagagag gcttttggtt tagtaataga
     4981 gggagttagt gagatgatta aatttgataa tattcaaatt aaatatggtg attttgttgc
     5041 aattgataat ctgaatttag atatacatga aggggaattc tttacatttc ttgggccttc
     5101 aggatgtggt aaatcaacta ctttgagagc attggtaggt tttttagatc catcatcagg
     5161 aagtattgaa gttaatggaa cagatgtcac tcatttggaa cctgaaaagc gtggaattgg
     5221 tattgtattt caatcttatg cgctatttcc aactatgact gtttttgata atattgcatt
     5281 tggtttaaaa gttaagaagg tagctccaga tgttattaaa gctaaagtat cagcagtggc
     5341 agcaaaaatt aagatctctg atcaacagtt acagcgtaat gtatcagaat tatctggggg
     5401 tcaacaacaa agggtagcat tggctcgtgc tctggttctt gaacctaaaa ttctttgtct
     5461 agatgaacca ttgtcaaacc ttgacgcaaa attacgtgta gatttgagaa aagagttgaa
     5521 aagacttcaa aaagagttag gtattactac tttatatgtt actcatgatc aagaggaagc
     5581 cttgacttta tctgatagaa ttgcagtctt taacaatgga tacatcgaac aggtcggtac
     5641 accagtagag atttatcata attctcaaac tgaatttgta tgtgatttta ttggagatat
     5701 taatgttttg accgatgaaa cagtccacga agtattactg aaaaatacaa gcgttttctt
     5761 agaagataaa aaaggataca ttcgattaga gaaagttcga ttcaatcgtg aaactgaaca
     5821 agatttcatt ctaaaaggga caattattga tgttgagttt tctggagtta caattcacta
     5881 tacaataaaa gtttctgaaa gtcagattct taatgtaaca agtattgata gtcaggctgc
     5941 tattagatct gtcggagaaa gtgtggaatt atttatcaca ccatcagacg ttctgcaatt
     6001 ttaaggaggt gcagtatgcg tcataaatta aatttaaaag attggcttat tcgtttaggg
     6061 ttaatctggt tcttagtaac atttattatt tatccaaact ttgatctagt agtgaatgta
     6121 tttgtaaaag gaggagaatt ttcccttgat gctgtacatc gtgttctaaa atctcagagg
     6181 gcacttcaga gtattatgaa cagttttaag ttagcatttt cactcattat tacagttaat
     6241 gtcgtaggta ttctttgtgt tctatttaca gagtactttg atattaaagg tgctaaaatt
     6301 ttaaaattag gttatatgac ctctttaatt tatggaggag tggttttagc gactggatat
     6361 aaatttgtct atggtccata tggattgatt acaaaatttt tacaaaatgt tatcccttct
     6421 ttagacccta actggtttat tgggtatggt gcagtcttat tcattatgac attttcagga
     6481 actgctaatc atacattgtt tttaacaaat acaattcgaa gcgttgacta tcacactatt
     6541 gaggctgctc gaaatatggg agcaaaacca tttactgttt tccgaaaagt agtgttacca
     6601 accttaattc caactctatt tgcacttact attatggttt ttcttagtgg tttatctgca
     6661 gtagcagcac ccatgattgt tggtggtaaa gaatttcaaa ctataaatcc aatgattatt
     6721 acatttgcag ggatggggaa ttctcgtgat ttagctgcct tacttgcaat tattttaggt
     6781 attgcaacta caattttgct tactatcatg aataagatag aaaaaggtgg aaattatatt
     6841 tctatctcta agactaaagc gcctcttaaa aaacaaaaaa ttgcgtctaa gccttggaat
     6901 atcattgctc acattgtagc atatggattg ttcacagttt tcatgcttcc actaattttt
     6961 atagtattat actcatttac agatccagtt gcaattcaaa caggtaactt aacattatca
     7021 aactttactt tagaaaatta tcgcttattc tttagtaata gtgcggcatt ctctccattc
     7081 ttggtcagct ttatttattc tattattgct gcgacaacag caacaattct cgcagttgta
     7141 tttgctcgtg ttgtcagaaa acataaatct cgttttgatt tcttatttga atatggtgct
     7201 ctacttcctt ggttattacc aagtacgctt ttagcagtaa gtttattatt tacttttaat
     7261 cagccacaat ttcttgtctt gaatcagatt ttggtaggta gtttggtaat tctacttatt
     7321 gcatatatag ttgtaaaaat cccattttct tatagaatgg tacgtgctat tttatttagt
     7381 gttgatgatg agatggaaga tgcagcaaga agtatgggtg cttcaccttt ttatactatg
     7441 atgaaggtta tcattccatt tattttaccg gttgttctct ctgttattgc tttaaacttt
     7501 aactctttat taactgactt cgacttatct gtattccttt accatcccct agctcaacca
     7561 ttaggtatta cgattcgatc tgcaggtgat gaaacagcaa catctaatgc acaagctctg
     7621 gtatttgttt atacaattgt tctgatgatt atttctggaa cggtattata cttcacacaa
     7681 agaccggggc gtaaagtaag gaaataatca tgacagccac tagtcttggg ttatcaaata
     7741 ttgaaatagt tgtcaggatt gttttatcag tagtcattgg tagtataatt ggtttagaga
     7801 gagggagcaa atcccagcct gcaggcatcc gaacttatag tattgtttgt ctagctgcat
     7861 gtttgattat gatgacgaat gaatacgtat cttataaatt tgggacagga gatcctacac
     7921 gattaggagc tcaagttata tcaggtgtgg gttttctagg cgctggaacg attcttatta
     7981 cagataaaaa gaaaattaca ggtctgacaa ctgcagcagg catttgggct tcggcaggaa
     8041 ttggattagc tattggagta ggtttttatg agggagctct tttagtagcc atttctgttt
     8101 ggggtgtgat atccatgttc caaccactaa aaaaatatct acaaaatcgt tctaaaatga
     8161 ttgaattgta tatagtggtt aaatctacag aagcctataa tcgtgtattg gtttattgtg
     8221 cagaaaatgg tatcagaatg accgattcaa gaacagcgtt tggagatgtt aattctgaca
     8281 gaattgaata ctttgatgtt ccagataaaa aaatagcgtc atttattact ctggaattat
     8341 caggtagatt tgaacatctt cgactgatgg aagaaattgc taatattgta ggtgttattt
     8401 atgtcgaaga aattagctga aatttagtaa acaaagggac agatgttttt gtccttttgt
     8461 tttttagatg ttttaatcat ctaatttttg agta
//
LOCUS       DBERDK010000081         8188 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.081, whole genome shotgun sequence.
ACCESSION   DBERDK010000081 DBERDK010000000
VERSION     DBERDK010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8188
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.081"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            82..315
                     /locus_tag="ACL73L_001816"
     CDS             82..315
                     /locus_tag="ACL73L_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HGS0772742.1"
                     /translation="MDNIIDVSIPVAEVVDKHPEVLEILVELGFKPLANPLMRNTVGR
                     KVSLKQGSKLAGTPMDKIVRTLEANGYEVIGLD"
     gene            315..1649
                     /locus_tag="ACL73L_001817"
     CDS             315..1649
                     /locus_tag="ACL73L_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HGS0772743.1"
                     /translation="MTDERIHILRDILLELHNGASPESVQDRFDATFTGVSAIEISLM
                     EHELMNSDSGVTFEDVMELCDVHANLFKNAIKGVEVSDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYESMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTTAKSLPEVSISSVKEAFEAFATEFESMIFKEESIL
                     LMILLESFTQDDWLQIAEESDAYGYAIIRPSEKWVPERQIFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKEDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKAIMKGLREGTKDKY
                     EMWFKSESRGKFVHITYAAVHDENGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            1659..1913
                     /locus_tag="ACL73L_001818"
     CDS             1659..1913
                     /locus_tag="ACL73L_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HGS0772744.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKVGKGFHDLPEGLFGERNY"
     gene            2009..2404
                     /locus_tag="ACL73L_001819"
     CDS             2009..2404
                     /locus_tag="ACL73L_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HGS0772745.1"
                     /translation="MSKKLNRKKQLRNGLRRAGAFSSTVTKVVDETKKVVKRAEQSAS
                     AAGKAVSKKVEQAVEATKEQAQKVANSVEDFAANLGGLPLDRAKTFYDEGIKSASDFK
                     NWTEKELLDLKGIGPATIKKLKENGIKFK"
     gene            2687..3264
                     /locus_tag="ACL73L_001820"
                     /pseudo
     CDS             2687..3264
                     /locus_tag="ACL73L_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813803.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
     gene            3251..3811
                     /locus_tag="ACL73L_001821"
     CDS             3251..3811
                     /locus_tag="ACL73L_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HGS0772746.1"
                     /translation="MARTELPDKIETERLVLRVRTVADAEDIHAYASLPEVAYPAGFL
                     PVKTLEDEIYYLVHILPNRNQKENFPAGYGIVVKGTDKVIGSVDFNHRHEDDVLEIDY
                     TLHPDYWGRGYVPEAARTLIDLAFKDLGLHKIELTCFGYNLQSKRVAEKLGFTLEARI
                     RDRKDAQGNCCDDLRYALLKSEWEVI"
     gene            3811..4389
                     /locus_tag="ACL73L_001822"
     CDS             3811..4389
                     /locus_tag="ACL73L_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGS0772747.1"
                     /translation="MHLFIIGAPASGKMTIGQELSRLTDATLFYNHQAIDFALEIYQD
                     YTEEMWEFVRGITFSFLGASARNQRSVILTDVIDFSNQYHLIYLKQIQSLLDDYHQEI
                     LFVELETSLEECIRRNRTENRLKYKPLKRHIEVSEREIIETAETLQLNSQHQPNELNH
                     YFKINNTNLSAEEAAKQIQNKINKIEKGHTHV"
     gene            4382..7033
                     /locus_tag="ACL73L_001823"
     CDS             4382..7033
                     /locus_tag="ACL73L_001823"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HGS0772748.1"
                     /translation="MSKELSPKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGEG
                     ISRYDLGRESFLTKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VNLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGKNVILPIANKLIPIVGDEHADPEFGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAFEFSGMDRFEARKAVVAKLEEIGALV
                     KIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNADGEMYVGEEAPEGDGWTQDEDVLDTWFSS
                     ALWPFSTMGWPEVDSEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVL
                     IHGLIRDEQGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNEGLTLDVAHDNVTKVATGEAGNVTDRWILHNLNETIAKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEDDKVITRSVLLYTLDKI
                     LRLLHPIMPFVTEEIFGQISEGSIVTAAYPTVNLAFEDLAAHTGVESLKDLIRAVRNA
                     RAEVNVAQSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASTIPAPELAMSS
                     VITGAEIYLPLADLLNVEEELARLEKELAKWQKELDMVGKKLFNERFVVNAKPEVVQK
                     ERDKQADYQAKYDATVARIDEMKKLVK"
     gene            7114..>8188
                     /locus_tag="ACL73L_001824"
     CDS             7114..>8188
                     /locus_tag="ACL73L_001824"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HGS0772749.1"
                     /translation="MGKIVAIDLFSGAGGTTSGLKKSGIDVQVAVEIDSVAVKTYKLN
                     NPEVSVIDKDINVVSGDEVKEYLKIDTDDKLMLVACPPCQGFSTIGTNNENDERNQLV
                     FQFLRLINELNPDFLLMENVVGMTRTRNEKIFKKFLTSIDNNYSVIYDILNTADYGVP
                     QSRKRLVLHGIRKEILDNTNLTLNLPSKTHSNIPELDLLSWINADIIMDLPPIEGGEE
                     YIGEGIYNHVANKLSNLNIERITHIRNNGGSRTSLPKSLELNCHKNRLGHSDVYGILD
                     INKPSITITGGCMSYSKGRFGHPTQNRALSAREAARLQSFDDDYIFVGNRGQLAKQIG
                     NAVPVKLAQASGNYFMSVYREIAN"
BASE COUNT         2487 a         1579 c         1851 g         2271 t
ORIGIN      
        1 ctgttttgag caacctgcgg ctagcttcct agtttgctct ttgattttca ttgagtatta
       61 gaacatacaa tggaggtcgt catggacaat atcatcgatg tgtcaattcc tgttgcagaa
      121 gtggtggaca agcatccaga agtcttggaa attctagtgg agttgggttt taaacccctt
      181 gccaatccct taatgcgcaa tacagttggt cgtaaagtat cacttaaaca gggttctaag
      241 ctagcaggaa ctcctatgga caagattgta cgcacactgg aagcgaatgg ctacgaagtg
      301 attggattag actaatgaca gatgaacgga ttcatatcct acgggatatt ttgttagaat
      361 tgcacaatgg cgcctctcct gagtcggttc aagatcgctt tgatgcgacc tttacgggcg
      421 tgtcagccat cgagatttcc cttatggagc acgagctgat gaactcggat tcgggcgtca
      481 cttttgaaga tgttatggaa ctctgtgatg tccatgccaa tctttttaaa aatgctatca
      541 aaggtgtcga agtttcagat actgagcatc caggtcaccc agttcgtgtc ttcaaagaag
      601 aaaatctggc tctccgtgcg gccttgattc gcattcgtag attgttagat acctatgagt
      661 ctatggaaga cgaggaaatg ctggcggaga tgcgtaaggg tttggtgcgt cagatgggac
      721 ttgtgggtca atttgacatc cattaccaac gtaaggaaga actcttcttt cctatcatgg
      781 agcgctatgg acacgattca cctcccaaag ttatgtgggg agtggatgat cagattaggg
      841 aactctttca aacagctcta acgacagcca agtcactacc agaagtgtca attagcagtg
      901 taaaggaagc ttttgaagct tttgcgacag agtttgaaag tatgattttc aaggaagagt
      961 ccatcctgct catgattctc cttgagtctt ttactcagga tgactggctt cagattgcgg
     1021 aggagagtga tgcctatggc tatgccatca tccgtccgtc agagaaatgg gtgccagaac
     1081 gacagatctt tattgaggaa aagattgcag aggagcctgt acaactagat acggcagaag
     1141 gtcaagttca acaagtcata gatacgccag aaggccattt taccattacc tttaccccta
     1201 aggaaaagga agctgtgctg gaccgccata gtcaacaggc ttttggtaat ggctatcttt
     1261 cagtcgagca ggccaatctc atcctcaatc atctccctat ggagattacc tttgtcaata
     1321 aagaagatat tttccagtat tacaatgaca atacgccagc tgatgagatg attttcaaac
     1381 ggacgccgtc ccaagtcggg cgcaatgtcg aactctgtca tccgcctaag tatttggaca
     1441 aggtcaaggc tatcatgaag ggacttcgtg aggggaccaa ggacaagtat gaaatgtggt
     1501 tcaagtctga gtcgcgagga aagtttgtcc acatcaccta tgctgcagta cacgatgaaa
     1561 acggagaatt ccaaggtgta ctggaatacg ttcaggatat ccagccctac cgtgagattg
     1621 atacggacta tttccgtgga ttagaataag gagaaaaaat gagttacgaa caagaattta
     1681 tgaaggaatt tgaagcttgg gtcaataccc aaatcatgat taacgacatg gcgcacaagg
     1741 aaagtcaaaa agtttacgaa gaagaccagg acgagcgtgc caaagatgcc atgattcgct
     1801 acgagagtcg tttggatgct tatcagttct tgcttggtaa gtttgaaaac ttcaaagtag
     1861 gcaagggatt ccatgatttg ccagaaggct tgtttggtga gcgaaattat taaacgagaa
     1921 agattcttga tttttcacta aaatcttgat agaatgttta tgttaaatcc ttgtcagagc
     1981 agggattttt tattgaaagg attttatcat gtcaaagaaa ctcaatcgta aaaaacaatt
     2041 acgaaatggc ctccgtcgcg caggtgcctt ttcaagtact gtgactaagg ttgtagatga
     2101 gacaaaaaaa gtcgtgaagc gtgcagaaca gtcagcaagc gcagctggta aggctgtttc
     2161 taaaaaagtt gaacaagcag tagaagctac caaagagcaa gctcaaaaag tagctaattc
     2221 tgtagaagat tttgcagcaa acttgggtgg acttccactt gatcgtgcca agactttcta
     2281 tgatgaagga atcaagtctg cttcagattt caaaaactgg actgaaaaag aactccttga
     2341 cttgaaagga atcggcccag ctaccatcaa gaaattgaaa gaaaatggca tcaagttcaa
     2401 gtaatttttc ttgagccttg catttccgag aaaaacttgc tacaatagag ccattagagg
     2461 tgttttgaat cccacatttt acagaaagtg gcagcgctga gaagtccaca aatgtgtcaa
     2521 aactggttgc taatgactga aaaaatgaaa tatttctgtc tttttatttt aaagactaaa
     2581 gatgcgggct tggcccgaaa ttgggtggta ccgcggataa acacattcgt ccctgtcatg
     2641 tagatggcag ggcttttctt ttgtgtctta gtcaaaggag gttgttataa aacaatcatt
     2701 taaaactaat aaactatatt atggttttcc tgtttttatt ttaggatatc aagatcagaa
     2761 ttttagctat aacatcatga cctgtagttc ctcttatagt ctaggagatt gggttgtgat
     2821 tggagtcgtt gcgagagaga atgccgcaga gcagatcaaa caatatcaaa aatttactgt
     2881 gaatatttct gatgaaactt ctatgcttgc gatggagcag gctggtttta tcagtcatca
     2941 ggagaaattg gaacgtttgg gagtgcatta tgaaatttct gaacgaactc agactcctat
     3001 tttagacgcc tgtccacttg ttttagattg tcgggtagat aggattgttg aggaagacgg
     3061 tatttgccac atctttgcca agattcttga gcgacttgtt gccccagaac tcctggatga
     3121 aaagggacat tttaaaaatc aactgtttgc ccaacctatt tcatgggaga tggatatcag
     3181 cgcgtttatt gctatctgga caagcgtgta gatatgaagg gcagtttcat caaaaaagcg
     3241 agaaagaagg atggcaagaa ctgagctgcc agataaaatc gaaacagaac gtctcgtttt
     3301 acgagtccgc acagtggcgg atgctgagga tatccatgcc tatgctagtt tgccagaggt
     3361 cgcctaccca gcaggttttc tgcccgtcaa gaccttggaa gatgagattt actatctggt
     3421 gcatatcctt cccaatcgca atcaaaagga aaatttcccg gctggctacg gaattgtcgt
     3481 caaaggaacc gataaagtca ttggttctgt tgatttcaac catcgccatg aagatgatgt
     3541 gctggaaatc gactatacct tacacccaga ctattggggg cgaggttatg tgccagaagc
     3601 tgcgcgtacc ttgattgact tagcctttaa agatttgggt cttcacaaga ttgaactaac
     3661 ttgctttgga tataaccttc aaagtaaacg agtcgcggaa aagcttggct ttaccctcga
     3721 agctcgcata agagaccgca aagatgctca aggcaactgc tgtgatgatt taagatatgc
     3781 cttgctgaag agtgaatggg aggtgatttg atgcatcttt tcatcattgg tgctccagca
     3841 tcaggaaaaa tgaccattgg tcaagaatta tctcgactga cggatgctac cctattttat
     3901 aaccatcaag ccatcgattt tgcactagaa atctatcagg actatacaga ggaaatgtgg
     3961 gaatttgttc gtggaatcac tttttcattc cttggagcga gtgcaagaaa tcagcgatca
     4021 gtaattttaa cagatgtaat tgatttttca aatcagtatc acctgatata tttaaagcaa
     4081 attcagagtt tgttggatga ttatcatcaa gagattcttt ttgttgagtt ggaaacaagt
     4141 cttgaagagt gcatacgtcg aaatcgaacg gagaatcgat taaagtacaa acccttaaaa
     4201 cgacatattg aggtatctga aagagaaatt atagagaccg ctgaaacact tcaattaaat
     4261 tcccaacatc aaccgaatga gttgaaccac tactttaaaa taaataatac gaatttgtct
     4321 gcagaagaag ctgctaagca gattcaaaat aaaataaaca aaatagagaa aggacacaca
     4381 catgtctaaa gaactttcac ctaaatacaa tccagccgag gttgaggctg gtcgttacca
     4441 aaaatggctt gatgccgatg ttttcaagcc ttcaggcgat caaaaggcta agccttattc
     4501 aatcgtgatt ccaccaccaa acgtaactgg gaaactccac cttggtcacg cttgggatac
     4561 aactttgcaa gatattatca tccgtcaaaa acgcatgcaa ggttttgata ccctttggct
     4621 tcctgggatg gaccacgcag ggattgccac tcaggctaag gttgaggagc gcttgcgtgg
     4681 tgagggcatt tcccgctatg accttggtcg tgagtctttc ttgacgaaag tctgggaatg
     4741 gaaagacgaa tatgccacta ccatcaagga acaatggggc aagatggggc tctctgtaga
     4801 ctattctcgt gagcgtttca ctcttgacga aggtttgtca aaagctgttc gtaaggtctt
     4861 tgtgaacctt tacaagaaag gctggatcta ccgtggtgag tttatcatca actgggaccc
     4921 agcagctcgc acagcccttt ctgatattga ggtgattcac aaggatgtgg aaggtgcctt
     4981 ctaccacatg aattacatgc tggaagatgg ttcacgcgcc cttgaagttg ctacaactcg
     5041 tcctgagact atgtttgggg acgttgcggt tgcggttaat ccagaagacc cgcgctacaa
     5101 ggacttgatt ggtaaaaacg tcatccttcc aatcgctaat aaactcatcc caatcgttgg
     5161 agatgagcac gcagatcctg agtttggtac tggtgtcgtg aaaatcacac ctgcccacga
     5221 tccaaatgac ttcttggttg gccaacgtca taacttgcca caagtcaacg tcatgaacga
     5281 cgacggaact atgaatgagc ttgcctttga attttcaggc atggatcgtt ttgaagctcg
     5341 taaggcagtc gttgctaagt tggaagaaat cggtgccctc gtcaaaatcg aaaaacgtgt
     5401 ccacagtgtt ggtcactcag agcgtacagg tgttgtggtt gaacctcgct tgtctactca
     5461 atggttcgtc aagatggacc aattggctaa gaacgccatt gccaaccaag acacagagga
     5521 caaggtcgaa ttctacccac ctcgtttcaa cgataccttc cttcaatgga tggaaaatgt
     5581 ccacgactgg gttatctctc gtcagctctg gtggggtcac caaatccctg cctggtacaa
     5641 tgctgatggt gaaatgtatg tcggcgaaga agctccagaa ggtgacggat ggactcagga
     5701 cgaagacgtc ttggatactt ggttcagttc tgccctctgg ccattttcaa ccatgggctg
     5761 gcctgaagtc gactcagaag actttaaacg ttatttccca acttcaacct tggtaacagg
     5821 ttacgacatc atcttcttct gggtgtctcg tatgatcttc cagtccttgg aatttactgg
     5881 tcgtcagcca ttccaaaacg tgcttatcca cggtctcatc cgtgacgagc aaggacgcaa
     5941 gatgtctaaa tctctcggta acgggattga cccaatggat gtcatcgaga aatacggtgc
     6001 tgatgccctt cgttggttcc tttcaaacgg ttccgcacca ggtcaagacg ttcgtttctc
     6061 ttacgagaaa atggatgctt catggaactt tattaacaaa atttggaaca tttctcgcta
     6121 tatcctcatg aacaatgaag gtttgacgct ggatgtggcg catgacaatg tcacaaaagt
     6181 tgcaacaggt gaggctggta atgtgacgga ccgctggatt ctccacaatc tcaacgaaac
     6241 cattgcaaaa gttactgaaa actttgataa gttcgagttt ggtgtggctg gtcatatcct
     6301 ttacaacttc atctgggaag aatttgccaa ctggtatgtt gagttgacca aggaagttct
     6361 atacagcgac aatgaagacg ataaggtcat tactcgatct gttctcctct atacgctgga
     6421 caagatcctt cgtctccttc acccaatcat gccattcgtg acagaggaaa tctttggaca
     6481 aatctcagaa ggttctatcg tgacagctgc atacccaact gttaatctag cctttgaaga
     6541 ccttgcggct cacactggtg tggaaagtct caaagacttg attcgtgctg ttcggaatgc
     6601 gcgtgcggaa gtaaacgtag cacaaagcaa acctatcacc atccttgtta agacaagcga
     6661 tagcgacttg gaagccttct ttaacagcaa tgtcaactat atcaaacgct ttacaaatcc
     6721 agaacacttg gaaatcgcat caaccatccc tgcacctgaa ctggctatgt caagtgtcat
     6781 cacaggagca gaaatctacc tgccactggc cgatctcctc aatgtcgaag aagaattggc
     6841 acgtctggaa aaagaacttg ccaaatggca gaaagaactg gacatggttg gtaagaaact
     6901 ctttaacgaa cgcttcgtag tcaacgccaa accagaagtt gtccaaaaag aacgcgacaa
     6961 acaagctgac taccaagcca agtatgatgc gaccgtagca cgtattgatg agatgaagaa
     7021 gttggtgaaa tgatgttaaa aacctaaccc tatcgggttg ggttttacat ttgataatgt
     7081 tataataaaa aggagaaact ttaggagaat actatgggga aaattgttgc tattgattta
     7141 tttagcggtg caggtggcac tacaagtggc cttaaaaaat caggcataga tgttcaagtt
     7201 gctgttgaaa ttgattccgt agctgttaaa acttataagt taaataatcc tgaggtgtct
     7261 gtaatagata aagatataaa tgtagtatca ggagatgaag taaaagagta tcttaaaatt
     7321 gatacagacg ataaactaat gctcgtggca tgcccaccat gtcaaggttt ttcaactatt
     7381 ggtactaata atgaaaatga cgagaggaat caacttgttt ttcaatttct tcgtttgatt
     7441 aatgaattga atcctgattt tctattaatg gaaaatgttg tgggtatgac tagaacaaga
     7501 aatgaaaaaa tctttaaaaa atttttgaca tcaatcgata ataattatag tgttatttat
     7561 gacattttaa atacagctga ttatggagtt cctcaatcta gaaaaagatt ggtgttacac
     7621 ggaattagaa aagaaattct tgataacacg aatttaactt tgaatttgcc ttccaaaact
     7681 cattcaaata taccagaatt ggaccttctt tcatggatta atgcggatat aataatggac
     7741 ttgcctccta ttgaaggtgg tgaagaatat attggagaag gtatctacaa tcatgttgca
     7801 aacaaactat ctaatttaaa tattgagaga attactcata ttagaaataa tgggggaagt
     7861 agaacctctt tgccaaaatc tttagaatta aattgtcata aaaatcgttt aggtcatagt
     7921 gacgtatatg gaatattaga tatcaacaaa ccttccatta ctattacagg agggtgtatg
     7981 tcatattcaa aaggtagatt tgggcaccca actcaaaata gagctttaag tgcaagagaa
     8041 gctgcaagat tacagtcttt tgatgatgac tatatttttg ttggaaatag aggtcaatta
     8101 gctaagcaaa ttggtaatgc agttccagta aaattagctc aagctagtgg aaattatttt
     8161 atgtcagttt atagggagat agcaaatg
//
LOCUS       DBERDK010000082         7732 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.082, whole genome shotgun sequence.
ACCESSION   DBERDK010000082 DBERDK010000000
VERSION     DBERDK010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 7732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7732
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.082"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..281
                     /locus_tag="ACL73L_001825"
     CDS             <1..281
                     /locus_tag="ACL73L_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGS0772750.1"
                     /translation="EFNRLSNVFSEEHIYYAHSYASWERGTNENHNRLIRRWLPKGTK
                     KMTPKEVAFIEKWINNYPKKYLDYKSPREDFWMANLNLKFSVRNKSRN"
     gene            277..951
                     /locus_tag="ACL73L_001826"
     CDS             277..951
                     /locus_tag="ACL73L_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HGS0772751.1"
                     /translation="MDSNFAGRLNILRAGVLDANDGIISIAGVVIGVASATTNIWIIF
                     LSGFAAILAGAFSMAGGEYVSVSTPKDTEEAAVSREKLLLDQDRELAKKSLYAAYIQN
                     GECKTSAQLLTNKIFLKNPLKALVEEKYGIEYEEFTNPWHAAISSFVAFFLRSLPPML
                     SVTIFPSEYRIPATVLIVGVALLLTGYTSARLGKDPTRTAMIRNLAIGLLTMGVTFLL
                     EQLFNI"
     gene            complement(993..2981)
                     /locus_tag="ACL73L_001827"
     CDS             complement(993..2981)
                     /locus_tag="ACL73L_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS0772752.1"
                     /translation="MFRLTNKLAVSNLIKNRKLYYPFALAVLLAVTITYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKELGIYGMLGLEKRHL
                     ISMTFKELVLFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQMKVVITVLL
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKKGRFLPLQTILGSISLGIGYYLAL
                     TVKDPLTALTTFFIAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSAESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDKGYKIKEKEVLRYTYFAVANQEGTKLTIFEKGQNRVQPKTVFMVFDQK
                     DYENMTGQKLSLSGNEVGLFVKNDGLKGQKTLTLNDHQFSVKEEFNKDFIVNHVPNKF
                     NILTTDYNYLVVPDLQAFLDQFPDSAIYNQFYGGMNVDASEEEQLKVAEEYENYLNQF
                     NAQLDTEGSYVYGSNLADASSQMSALFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQTINKQVLTVFFLPLLFAFIHLAFAYHMLSLILKVIGV
                     LDTTMMLIVTLSICAIFLIAYVLIFMITSRSYRKIVQM"
     gene            complement(2983..3741)
                     /locus_tag="ACL73L_001828"
     CDS             complement(2983..3741)
                     /locus_tag="ACL73L_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0772753.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPSRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVMTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSAALLDVFNEINERGQTILMVTHSTAAASRAKRVLFIK
                     DGILYNQIYRGEKTERQMFQEISDTLTVMASEVN"
     gene            4169..4360
                     /locus_tag="ACL73L_001829"
     CDS             4169..4360
                     /locus_tag="ACL73L_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772754.1"
                     /translation="MKHKEHILIGLLYLLSPFIGQLLVEHTHFISTEFTGTAYVICWL
                     SVVISIHHFSKNVLSQQQK"
     gene            complement(4714..4986)
                     /locus_tag="ACL73L_001830"
     CDS             complement(4714..4986)
                     /locus_tag="ACL73L_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HGS0772755.1"
                     /translation="MNSKFYKMRRNRMISGVLAGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVIIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGWFW"
     gene            complement(5044..5493)
                     /locus_tag="ACL73L_001831"
     CDS             complement(5044..5493)
                     /locus_tag="ACL73L_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HGS0772756.1"
                     /translation="MKLTDYVKQVSLEDFGRPFIHHVQWNRRLRSTGGRFFPKDGHLD
                     FNPKVYQELGLDVFRKIVRHELCHYHLYFQGKGYQHKDRDFKELLKAVDGLRFVPSLP
                     NSNSKPIKLYRCQSCQQTYQRKRRIDTKRYRCGLCRGKLLLVNQPED"
     gene            complement(5480..7609)
                     /locus_tag="ACL73L_001832"
     CDS             complement(5480..7609)
                     /locus_tag="ACL73L_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HGS0772757.1"
                     /translation="MDKKYEKISQDLGVTLKQIDTVLSLTAEGATIPFIARYRKDMTG
                     SLDEVAIKAIIDLDKSLTNLNDRKEAVLAKIQEQGKLTKELEEAILVAEKLADVEELY
                     LPYKEKRRTKATIAREAGLFPLARLILQNIVDLEKEAEKFVCEGFATGKEALTGAVDI
                     LVEALSEDVTLRAMTYQEVLRHSKLTSQAKDESLDEKQVFQIYYDFSETVGTMQGYRT
                     LALNRGEKLGVLKIGFEHATDRILAFFATRFKVKNAYIDEVVQQSVKKKVLPAIERRI
                     RTELTEKAEEGAIQLFSDNLRNLLLVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLT
                     TQVIYPVKPASARQIEEAKKDLADLIGQYGVEIIAIGNGTASRESEAFVAEVLKDFPE
                     VSYVIVNESGASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVSQKKLSESLDFVVDTVVNQVGVNVNTASPALLSHVAGLNKTISENIVKYRE
                     EEGKITSRSQIKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYAAVKELFKCL
                     DIKDLNEEAQSKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDSFDAPVLRQD
                     VLDIKDLVVGQKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVAVGD
                     LVSVWVKKIDTEREKVNLSLLAPNETD"
BASE COUNT         2307 a         1624 c         1509 g         2292 t
ORIGIN      
        1 cggaattcaa tcgcttgtct aatgtatttt ctgaggagca catttattat gcacattcct
       61 atgcctcttg ggaaagggga actaatgaga atcacaacag gctcattcgt agatggttac
      121 ctaagggaac caagaaaatg actcccaaag aagtcgcatt catcgaaaag tggatcaaca
      181 actatcctaa aaaatacttg gactacaagt cacccagaga agacttctgg atggctaact
      241 tgaacttgaa atttagcgtc agaaacaaat cacgaaattg attcaaattt tgcaggtcgt
      301 ttaaatatcc tgcgtgcggg tgttcttgat gctaacgatg gaattatttc cattgctggt
      361 gtggttatcg gagttgccag tgccacgacc aatatctgga ttatcttttt atcaggtttt
      421 gcggctatct tagctggtgc cttttcaatg gctggtggag aatatgtatc cgtttcaact
      481 ccaaaagata ccgaggaagc tgccgtttcg cgagaaaaac tcttgttaga ccaagatagg
      541 gaactagcca aaaaatccct ctatgctgct tatatccaaa atggagaatg caaaacttct
      601 gcccaactct tgaccaataa gatctttctt aaaaatccac tcaaggctct ggtagaggaa
      661 aaatatggga ttgagtatga agaatttacc aatccttggc acgctgccat ttctagcttc
      721 gttgcctttt tccttagaag tttgcctcca atgctgtcag tgaccatatt cccaagtgaa
      781 tatcgcatcc ctgctactgt ccttattgtc ggtgtggccc ttcttctcac tggttacact
      841 agtgccagac ttggaaagga tccgactaga acagctatga ttcggaacct tgctattggt
      901 ctcttgacca tgggagttac cttcctgctc gaacaacttt tcaacattta gaatacaaga
      961 aatacctcga ttttgaagtc gaggtatctt ttttacattt gcacaatctt gcgataactt
     1021 cttgaagtaa tcatgaaaat cagcacatag gcgatgagga agatagcgca gatagacaag
     1081 gtcacaatca acatcatagt cgtatccagt acaccaatca cttttaaaat caggctaagc
     1141 atatggtagg caaaggcgag atgtatgaag gcaaagagca aaggaaggaa gaaaacagtt
     1201 aaaacctgtt tgttgatggt ttgcttgatt tgcttttggt ccaaaccgac tttctgcaag
     1261 ataataaagc gttcacggtc ttcgtagcct tcagaaattt gtttgtagta gatgaccaga
     1321 acagttccga ccataaagat aatggatagg aaaataccga taaagaagac accaccaaag
     1381 agggcactca tctgagaact agcatctgct agattgctac cataaacata gctaccttct
     1441 gtgtctaatt gagcattaaa ttgattgagg tagttttcat actcctcagc gaccttgagt
     1501 tgttcttctt cgctggcatc tacattcata ccaccgtaaa actgattata gatagccgaa
     1561 tctgggaatt gatccaaaaa ggcttgtaaa tcaggaacaa caaggtaatt gtaatcagta
     1621 gtcaagatat taaacttatt tggaacatgg ttgacaatga aatctttatt aaattcttct
     1681 ttgacagaaa attgatgatc atttagagtt agagttttct gtcctttcag tccgtcattt
     1741 ttgacaaaga gaccgacctc atttcctgat agagacagtt tttgaccagt catattttca
     1801 taatcttttt ggtcaaatac catgaaaact gtttttggtt ggacacggtt ttgtcctttt
     1861 tcaaaaatag ttaacttggt tccttcttga ttcgcaacag caaagtaagt gtaacgaagc
     1921 acttctttct ctttaatctt ataacctttg tcacttgcaa actggctcaa gagtttgtcc
     1981 aaatcttctt tttcaacatt ttgccctgaa accccaaaat catgaggatt tagaactttt
     2041 ttaaaggatt ctgcggaatt gaaaatgctt gtcgctgctg acatggttac caaaaccatt
     2101 gttgacaaaa tagcgatagt tgctagtcca actgcatttt tcttcatacg gaaaatcagg
     2161 ttggaaacag agatgaggtt attaggttgg taatagtatt tcttgttttt cttcaagatt
     2221 tgaagaaaaa cggtaatccc tgcattgaac aaaagataag taccaaagat aaccagtaaa
     2281 acagctatga agaaggttgt taaggctgta agaggatctt ttaccgtaag ggcaagataa
     2341 tatccaatcc ctaaacttat ggaaccaaga atagtttgga gaggaaggaa gcgacctttt
     2401 ttctctccgc tagctttctc acgtgagagc tggagggcat tcatacgggc gattcgaagg
     2461 gcattcagga acatgaggcc taggaaaatc aaaccgaaga caagaagtac tgtaatgaca
     2521 actttcatct ggaaggtagc gaccagttcc accttcaatt tcatcagttt gagtaggaaa
     2581 gcgaaaatca acttgtcaaa caaggctcca ataccgattc ctgctccaac agttagaatc
     2641 ccaaatagca ctaactcctt aaaggtcata ctgattagat ggcgcttctc caagcctaac
     2701 atgccatata tacccagttc cttggaacgg tttttcatga caaaactatt ggcatagagg
     2761 acgataatgg ctgacgcaag ggtaacgaca aacataccaa atccaagtgt tgcttgaatg
     2821 gtggttcctc cacggatttc cgcaatcttg ggattgaagg ttagggagta aaagagatag
     2881 gtgatggtga ctgccaagag aacagccagt gcaaagggat agtagagctt gcggttttta
     2941 atcaagttcg ataccgctaa cttattggtt aatcgaaaca tactaattca cctcgcttgc
     3001 catgacagtc aaggtatcag agatttcttg gaacatctga cgctctgtct tctctccacg
     3061 gtagatttgg ttgtaaagaa tgccgtcttt gataaagaga acacgcttgg ccctgctagc
     3121 agctgctgtt gagtgggtta ccatgaggat ggtttgccca cgctcattga tttcattaaa
     3181 gacatcaagt aaggctgcag atgacttgga atcaagggct cctgttggct cgtccgcaag
     3241 gagaatttca ggttctgtga tgatggcgcg ggctactgct acacgctgtt tctgaccacc
     3301 agaaatctcg taagggtact tctcttgcaa ttggttgata cccagatttt cagctgtcat
     3361 caccaatttc ttcatcatct ccgttatagg tcttcttgac aagacaagcg gaagcaagat
     3421 attgtcctta acagacagag tatctagcaa gttaaagtct tggaagacaa atcctagctt
     3481 ttcacgccgg aaactagaag cctgtgaatt tttaatagtt gctgtatcag ttccatttaa
     3541 gtaaacctga ccacgacttg gtttgtccag catagctagg atattgagaa gagtggattt
     3601 accagaacca gactcaccca tgatggcaac atagtcaccc ttttctacgg taaagtgaat
     3661 atccttgagg gcctctactt ggttgccctg aaaacgtgtt ttataaattt tttgaacgtg
     3721 ttttacatct aaaagtgtca tgagaatctc ctttcttaat atcccatcaa atgaaatgta
     3781 gcttgcatca tagtgcatcc cagctgaacg cgctcaattt ctttgtgact tggttttcta
     3841 gttttcttat gtaagatttg tttcatgatg ttactccttt gttctttatg agtctagttt
     3901 acatcaaaaa aagaccgctt gccataacct aaccttacaa aagagacttt aatcttacat
     3961 ttttgtaaga ttgggtgaaa tataagcaaa attacttaga aaatgatttg atttcctatt
     4021 tttaaagttg taaaatataa gtgtgaaaag gcttactttg aaaaggagga agcgggaact
     4081 aacattttag taaggttacc tagataacat accattcaat ttaacacttg caagtcgttc
     4141 aacctataag gaaagaaggt gcgctttaat gaaacacaaa gaacatatac tcatcggact
     4201 tctctatctg ctctctccat tcatcggtca gctcttagtt gaacacacac actttatcag
     4261 tacagaattt accggcactg cttatgttat ttgctggtta tcagttgtta tcagcatcca
     4321 ccatttttcc aaaaatgtat tgtctcaaca gcaaaaatag attaaaaaac tgcgtttaaa
     4381 ttactgcttt tatagtaaaa tgaaataaga acaggacaaa tcgatcagga cagtcaaatc
     4441 gatttctaac aattttttag aagtataggt gtcctattct agttccaatc cactatagtt
     4501 tgtggatgaa gctggcgaaa tattattaat actattcaaa aatcaaagtg caaactatat
     4561 caacttcaca ttgccgacta aattacagcc tgcggctggc ttcctagttt gcactttgat
     4621 ttttattgag tataaaagta aaaaagcttt aaatcaagca gtttagacta ttgatttaaa
     4681 gctttttgcg tattccaaat cttttgtaaa acctcaccaa aaccagcctt cattatcgtc
     4741 aatggcttga gcttctttgc gtttacgtgg gcctgttccg tacatttctg cttcgatttc
     4801 ttccttggtt ggcatgatag aagctaggat gatatagata atcaccccaa gtccaaagtt
     4861 tgcgactgta aaaatggcaa acagaaaacg tacaagggta acatcaaagt tccacttgtc
     4921 tgacagacct gctaaaactc ctgaaatcat gcgatttcgt ctcattttat aaaacttact
     4981 gttcatgata tttcctcttt ctgctaggtt agactagtat atcacggata ggctcggctt
     5041 tcatcagtcc tcaggctgat ttactaatag caactttcct cgacaaagtc cacagcgata
     5101 gcgtttggta tcaatcctac gcttacgctg ataagtttgc tggcaggatt ggcaacgata
     5161 gagcttgatt ggcttggagt tactattggg caaggatggt acaaaccgta atccatccac
     5221 tgctttcaac agttccttaa aatcccgatc cttgtgttga tagcctttcc cttgaaaata
     5281 gaggtgataa tgacagagtt catgtcggac aattttccta aaaacgtcca accccagttc
     5341 ctgataaacc ttgggattaa aatccaaatg cccatctttg gggaaaaatc gcccacctgt
     5401 cgaacgtaga cgcctattcc actggacatg atggataaaa ggtctgccga agtcttctag
     5461 tgaaacctgc ttgacgtaat cagtcagttt catttggagc taggagagac agattaactt
     5521 tttcacgttc agtatcaatt ttcttaaccc aaacgctcac caaatctcca actgccacca
     5581 cttgactagg gtgtttgata aacttgcgac tcatatggga aatatggatg agaccgtcct
     5641 catgaattcc gatatcaaca aaagcaccga aatcaacaac gttacgcacc actccttcta
     5701 gcttttgtcc aaccactaag tccttgatat ctaggacatc ttgacggaga actggagcat
     5761 caaaggaatc acggaaatct cgacctggtt tgagaagatc tgcaatgata tctttaagag
     5821 tttctggacc aaggtctaac tcttgcgcca tttccttgac tgaaagcgac ttgagtttgc
     5881 tttgggcctc ttcattcaga tccttaatgt ccaagcattt gaagagttcc ttaacggcag
     5941 cgtaattttc tgggtgaact cctgtattat caaggatatt gctactttca gggatacgga
     6001 ggaaaccagc agcctgttca aaagccttgg cccccaaacg aggaactttc ttgatttggg
     6061 agcgtgaagt gatttttccc tcttcctcac ggtatttgac gatattttca gaaatggttt
     6121 tattgagtcc agctacgtgt gaaagaagag ctgggctagc tgtattgaca ttgacaccaa
     6181 cttggttaac cactgtatcg acaacaaagt ccagactctc agatagtttc ttctgactga
     6241 catcgtgttg gtattgaccg acaccaattg acttaggatc gattttgacc aattccgcaa
     6301 gaggatcttg caaacgacgg gcgatagaaa tggcagagcg tttttcaacg gtcaagtctg
     6361 gaaactcctg acgagcaagt tcgctggcag aatagacaga agcaccactt tcattaacga
     6421 taacatagct gacttcaggg aaatctttca gaacttccgc tacaaaagct tcactttcac
     6481 gactggccgt tccatttcca atggcaataa tctctacacc gtattgacca attaaatctg
     6541 ctaaatcttt cttggcttct tcgatttgac gagctgatgc tggtttaaca ggataaataa
     6601 cctgagttgt cagcattttt cctgttgcat ccacgacagc tagcttggca cctgtacgaa
     6661 aggctgggtc aaacccaaga accacgcgcc ctttcagtgg agcaaccaag aggagattgc
     6721 gcagattgtc agaaaaaagt tggatagctc cctcttcagc tttctcagtt aattctgtcc
     6781 gaatacgacg ctcaatagca ggcaagacct ttttcttaac ggattgctga acaacttcat
     6841 caatataagc atttttcacc ttgaaacgag tagcaaagaa ggcaagaata cggtccgtcg
     6901 catgttcaaa accgatcttc aagacaccaa gtttctcccc acgattgaga gccaaggtac
     6961 gatagccttg catagttcca actgtctctg aaaaatcata ataaatctga aaaacctgct
     7021 tttcatcaag actttcatcc ttggcttgag aagtgagttt agagtgtctc agcacttcct
     7081 gataagtcat agcacgcaat gtcacatctt ccgataaggc ttcgaccaaa atatcaactg
     7141 caccggtcaa ggcttccttg ccagtcgcaa atccttcaca gacgaacttt tcagcttctt
     7201 tctctaagtc aactatattc tgcaaaatca agcgagcaag aggaaagagt ccagcttcac
     7261 gggcaatggt tgccttggta cgacgctttt ccttataagg aagatagagt tcttcaacgt
     7321 ctgctaattt ttcggcaact aagatagctt cttccaattc cttggtcaac ttaccttgtt
     7381 cttgaatctt agctaagaca gcttccttac ggtcattgag atttgtcaga cttttatcca
     7441 aatcaataat agccttaatc gccacctcat ccagactacc agtcatgtcc ttgcgataac
     7501 gcgcgataaa gggaatagtc gccccttcag ctgtcaaact tagaacggta tcaatttgct
     7561 ttaacgtcac tcccaaatcc tgagagattt tttcatattt tttatccata aatctattat
     7621 accacaagct ggaaggcttt ctccaatcct tttcatactc aatgaaaatc aaaaagcaaa
     7681 ctaggaagct agccgcaggc tgctcaaaat actgttttga ggttgtggat aa
//
LOCUS       DBERDK010000083         7693 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.083, whole genome shotgun sequence.
ACCESSION   DBERDK010000083 DBERDK010000000
VERSION     DBERDK010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 7693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7693
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.083"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..674
                     /locus_tag="ACL73L_001833"
     CDS             <1..674
                     /locus_tag="ACL73L_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HGS0772758.1"
                     /translation="NEIEYIEMLKQNKVDGIIGITYSDIDKYILSDLPFVSIDRHFSE
                     NVCYVTSANYKGGQTAAEELVRRDNKHLAFIGSINMYENETILRSKGFREKAKELGVD
                     VYNFEMKEPLINPEDQIRIFLQSHKEIDGIFTINDMMALRVISVMKELGKIAPDDYQI
                     IGFDGLKTSVDQSYLVSTIVQDIGMMAKESVAMILKMIKREEILEKRIVIPTYFWGDK
                     TTKKI"
     gene            801..2111
                     /locus_tag="ACL73L_001834"
     CDS             801..2111
                     /locus_tag="ACL73L_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HGS0772759.1"
                     /translation="MRKGTMLSVVAGLSLAILAGCSGGTNSKQASSSNDIKVWVQFSD
                     ETNEGKAWQKVVDNFNQSGKSKYKVVTEYIPRSGSGGGYEDKVNAAITTNSLPDVITL
                     DGPNTAAYAKSKVITPLDDYLKDNNMDDVLDSIKQQGTYNGKFYAFGFSESNVGVYYN
                     KKMFKEAGIAESELPTLEKPWTWDEFNTIAKKLKDHYNKPAIDFRINSNDEMLPYAYM
                     PLIWSNNGSVVNEDGTKAEGYFNSKESVEAVQFIQNLVKEGYTTVSPVEKGFETGEYP
                     MLLSGSWTIADMNTNYKDIDFGILPYPVSSKTKKLVSPSGSWQLAVTTKSDKKEAASE
                     FVKFATNTESSEIISLGNSVLPIRKSTIENIKDKVSEPMRFLMEQNSKTAHARPVVVA
                     YPQVSRAFQQAMQDISYYEENPNVQKVLDTRTKEMQTAIDQSLK"
     gene            2207..3109
                     /locus_tag="ACL73L_001835"
     CDS             2207..3109
                     /locus_tag="ACL73L_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGS0772760.1"
                     /translation="MSKETVVDTKRREKIKDNILGYSFLAPALILLGIFLVIPVGMVI
                     YYAFTDYYLLTPDERKFVGLENFIRLTKDPIFLKSFLNTLKFVVWIIPVQLGAALGMA
                     LIVNKKRKGNMFFKVAFFAPVVMSLVVISILWLYLLNPNSGLLNAILNKVGIASQPFL
                     TSPKQAMYAIVFVSAWQGAGYQMLLFLGGMQNIPQDVYEAAELDGFSKWAQFRYITMP
                     LLKPTALFVLLTTLISAFKLIVQPMVMTQGGPLNSTITMVYYIYQQGFTDRLVGYSSS
                     IALVFTTLIGMISLVQRRVLKEDD"
     gene            3111..3956
                     /locus_tag="ACL73L_001836"
     CDS             3111..3956
                     /locus_tag="ACL73L_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGS0772761.1"
                     /translation="MKRFKPTTILEYILLTLLAGLFLFPMVWMVVSAMKPEADVYKNL
                     TSWQAFLPSLNPADWFKPYQEVFERFSIFTYLGNSIFYAGTFAIGSIIVNALAGFAFA
                     KVNFSGKKILFGFLLALLIIPVETVLIPQFTIINSLGLVNNRLAVIIPGLASVFNIYL
                     FRNFFIAIPEEILESAKMDGASIVRIFFRIMLPMSKPAVATVGVLSFISSWNDYIWPL
                     MVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTISTIPLILVYVVAQKYIVQGLGGS
                     GTGIK"
     gene            3966..5285
                     /locus_tag="ACL73L_001837"
     CDS             3966..5285
                     /locus_tag="ACL73L_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HGS0772762.1"
                     /translation="MINKTFTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNG
                     FVYFRGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVTWEHLPVALAPDQDYDRNGCFSG
                     SAIVKDDRLWLMYTGHIEEKTGVRQVQNMAFSDDGIHFEKIEQNPVATGADLPDELIA
                     ADFRDPKLFEKDGRYYSVVAAKHKDNVGCIVLLGSDNLVEWQFESIFLKGGEHQGFMW
                     ECPDYFELDGKDCLIMSPMRYQREGDSYHNINSSLLFTGKVDWGEKRFIPESVQEIDH
                     GQDFYAPQTLLDDQNRRILIAWMQTWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQ
                     FPVKKGQYQIQIDKDCHYHLGNDTDYLEFGYDSNVQQVYIDRSHLIQKILGEEEQDTS
                     RRYVDIEAKELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID"
     gene            complement(5495..5567)
                     /locus_tag="ACL73L_001838"
     tRNA            complement(5495..5567)
                     /locus_tag="ACL73L_001838"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5532..5534),aa:Lys,seq:ctt)
     gene            complement(5832..7103)
                     /locus_tag="ACL73L_001839"
     CDS             complement(5832..7103)
                     /locus_tag="ACL73L_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HGS0772763.1"
                     /translation="MPDNLALRMRPKTIDQVIGQEHLVGPGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     IALSNPERGFDFPIELDEDALDFIATSTNGDLRSAFNSLDLAVLSTPENDEGIRHITL
                     DIMENSLQRSYITMDKDGDGHYDVLSALQKSIRGSDVDASLHYTARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQKIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALADLKTSGHLPIPRHLRDGHYSGSKELGNAQDYLYPHNYPGNWIKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDRLRKI"
     gene            7373..7612
                     /locus_tag="ACL73L_001840"
     CDS             7373..7612
                     /locus_tag="ACL73L_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772764.1"
                     /translation="MKREVISNGNDGPSQEILIFTKQIRHWILSDQVISGKRKLFFRE
                     DTPKEILDLYENIKSKLDFAYQEVHSNNGLKKYEK"
BASE COUNT         2454 a         1250 c         1601 g         2388 t
ORIGIN      
        1 aaaatgaaat cgaatatatc gaaatgttga aacaaaataa agtggatgga attattggta
       61 ttacatattc tgatattgac aaatatattt tatctgattt accgtttgta agtatagata
      121 gacatttttc ggaaaatgta tgctatgtta cttcagcaaa ttacaaagga ggacaaactg
      181 cagcagagga actggttaga agggataata agcaccttgc ttttattggt tcaattaata
      241 tgtatgagaa tgagactata ctcagaagta aaggattcag agaaaaagct aaggaattag
      301 gagtagatgt ctataatttt gaaatgaaag aacctttaat aaatcctgaa gatcagataa
      361 gaattttttt gcaatctcat aaagaaattg atggtatatt tacaataaat gatatgatgg
      421 ctctgagagt tattagtgtt atgaaagaac tggggaaaat agcacctgat gattaccaga
      481 taattgggtt tgatggttta aaaacttctg tcgatcaatc ttatctagtg tctacaatag
      541 ttcaagatat tggtatgatg gcaaaagaat ctgttgcaat gatactgaaa atgattaagc
      601 gagaagagat tctagaaaaa cgaatcgtaa ttcccacata tttttgggga gataaaacaa
      661 caaaaaaaat ttaaaaagcg cttgacaatt aatgaaagcg ctgttataat ataatcaaca
      721 aatgaaacgt ttcaaaaata ggctagaagc ttgtttaaaa aataaataaa aaatgaaacg
      781 tttcaaaaag gaggaaaatg atgcgtaaag gtacaatgtt atctgttgtt gcggggctat
      841 cgttagctat acttgcaggt tgtagtggag gaactaactc aaaacaagcg agttcttcaa
      901 atgatattaa agtctgggta caattctctg acgaaacaaa tgaaggaaaa gcttggcaaa
      961 aagttgttga taactttaat caatcaggaa aaagtaagta caaggttgta actgagtata
     1021 ttcctcgtag tggtagtggt ggaggatatg aagacaaggt taatgctgct attacgacta
     1081 atagtcttcc agatgttatt acccttgatg gaccaaatac ggcagcttat gcaaaatcta
     1141 aagtaattac tccgctagat gattatctaa aagataataa tatggatgat gttttggaca
     1201 gtattaaaca acagggaact tataatggga aattctatgc atttggattt tctgaatcga
     1261 atgttggtgt ttattacaat aaaaaaatgt tcaaagaggc cggaattgcg gaatcagagc
     1321 ttccaacgct agaaaagcca tggacatggg acgaatttaa tacgattgct aaaaaattga
     1381 aagatcatta taacaaacca gcaattgatt ttcgtattaa ttcaaacgat gaaatgttgc
     1441 catatgctta tatgccgttg atttggtcaa ataatggttc tgtcgtaaac gaagatggaa
     1501 caaaggcaga aggatacttc aactccaaag agagtgtaga agcagttcaa ttcattcaaa
     1561 atcttgtaaa agaaggctat acaactgtta gtccagtcga aaaaggtttt gaaacaggag
     1621 agtatccaat gcttttgagt ggttcttgga cgattgctga catgaataca aactacaaag
     1681 atattgattt tggtatcctt ccttacccag tatctagtaa gacgaaaaaa ttagtatccc
     1741 cttcaggtag ttggcaatta gctgtgacga caaaatctga taaaaaagag gccgcatctg
     1801 aatttgttaa atttgctaca aatacagaat caagtgaaat tattagcttg gggaattcgg
     1861 tccttccaat tcgtaaatct acaattgaaa atatcaaaga taaagtatcc gaaccaatgc
     1921 gtttcttgat ggaacaaaat tcaaaaacag ctcatgctcg cccagttgtt gtagcatatc
     1981 ctcaggtttc tcgagctttt caacaagcaa tgcaagatat tagctattat gaagaaaatc
     2041 caaatgtgca aaaagtcttg gatactcgta caaaagaaat gcaaactgct attgatcagt
     2101 cactcaaata atctgtaaaa aagaaggtgg aaggaaaatg tttggaacta ccttcccctt
     2161 tcttgttaat agcctagttt gatatatatg attaaaggag atggatatga gtaaagaaac
     2221 tgttgtggat acaaaaagac gtgaaaaaat aaaggataac atattaggtt atagtttttt
     2281 agctccagct ttaattttat taggtatctt tttagtaatt ccagttggaa tggtcattta
     2341 ttacgctttt acagattatt atttgttgac accagatgaa agaaaatttg ttggacttga
     2401 aaattttatt cgattaacaa aagatccgat tttcttaaag agcttcttga atactttgaa
     2461 atttgttgtt tggattattc cagttcaatt gggagcggcc ttaggaatgg ctttgattgt
     2521 caataaaaag cgaaaaggaa atatgttttt taaagtggct ttttttgctc cagttgtcat
     2581 gtccttggta gttatttcta ttctttggct ttatctttta aatccaaata gtggcttatt
     2641 aaatgctatt ttaaataagg taggtattgc ttctcaacct ttcttgacta gtcctaaaca
     2701 agctatgtat gcaattgttt ttgtttctgc ttggcagggt gcaggttatc agatgctttt
     2761 atttttagga ggaatgcaga atattccaca agatgtctat gaagctgctg aattagatgg
     2821 attctcaaaa tgggctcagt ttcgttatat cacgatgccc ctactaaaac caacagccct
     2881 gtttgtattg ttaacaactt taatttctgc ttttaagctg attgtacaac caatggttat
     2941 gacgcaaggt gggccattaa actcaacaat tactatggtg tactatattt atcaacaagg
     3001 ttttactgat cgtcttgtag gatattctag ctcaatcgct ttagtattta caacgttaat
     3061 tggtatgatt agcttagtac aacgtcgcgt tctgaaggag gatgactaag atgaaacgat
     3121 ttaaaccaac aacaatctta gaatatattc tgttaacttt attggcagga ctcttccttt
     3181 ttcctatggt ttggatggta gtttcagcta tgaagccaga agcagatgtt tataaaaatt
     3241 tgactagttg gcaagccttc ttaccaagtt taaatcccgc tgattggttt aaaccttatc
     3301 aggaagtatt tgagcgcttt agtattttta catatctagg gaacagcatt ttttatgctg
     3361 gaacgtttgc tattggttct attattgtca atgcactggc cggtttcgct tttgcaaaag
     3421 tcaatttttc aggtaagaaa attttatttg gtttcttact tgctttactt attattccag
     3481 tggaaactgt gttaattcca caatttacca ttatcaattc cttaggtttg gtcaataatc
     3541 gtcttgcagt tattattcca ggattggcga gtgtatttaa tatttactta tttaggaatt
     3601 tctttattgc aattccagaa gagattcttg agtctgcaaa gatggacgga gcaagtatcg
     3661 ttagaatttt ctttagaatt atgcttccaa tgtctaaacc tgcagttgct acagttggtg
     3721 ttttatcatt tatttcaagc tggaatgatt atatctggcc attgatggtt ttgacagatt
     3781 cttctaaatt ttcaatgcag gttgcaatta caactattaa tacaactcaa ccagtttata
     3841 tcaatcaggt aatggctgta ttaactattt caacgattcc gttgattttg gtctatgtag
     3901 tcgctcagaa atatattgtt caaggtctgg gtggttccgg aactggaata aaataggata
     3961 tacttatgat aaataaaaca tttacagtag aaaaagccaa tcgttttata gcagaaaata
     4021 aacatctcgt taatactcaa tataagcctg aagaacattt ttcagctgag attggttgga
     4081 tcaatgatcc aaatggattt gtttattttc gtggagaata ccatctcttt tatcaattct
     4141 atccatatga tagtgtttgg ggacctatgc actggggaca tgctaaaagt aaggacttgg
     4201 tgacttggga gcacttgcca gtggcacttg ctcctgatca agattatgac cgaaatggtt
     4261 gtttctcagg ctctgccatt gtcaaggatg atcgcctctg gctcatgtac actgggcata
     4321 tcgaagaaaa aaccggtgtc cgccaagtgc aaaatatggc attttcagac gacgggattc
     4381 actttgaaaa gattgaacaa aatccagttg caacaggagc agacttgcca gatgagttga
     4441 ttgctgctga tttccgtgat ccaaaactct ttgaaaaaga tggacgctat tactccgtag
     4501 tagctgccaa acacaaggat aatgtgggct gtatcgttct actagggtcc gataacctag
     4561 tagaatggca gttcgaatcc atctttttaa aagggggaga acaccaaggc tttatgtggg
     4621 aatgcccaga ttacttcgag ttagatggga aagattgcct tattatgtca cccatgcgtt
     4681 atcagcgtga gggagactca tatcataaca tcaactcatc gcttttgttc acgggtaagg
     4741 tagattgggg agaaaaacgt tttatcccag aatcagttca agaaattgat catggccaag
     4801 acttctatgc gcctcaaaca ttgttggacg atcaaaatcg tcgtatcctg attgcttgga
     4861 tgcagacatg ggggcgtacc cttccaactc atgaccaaga acacaagtgg gcatgtgcca
     4921 tgactttacc tagaattcta agattggaag atggcaaact aagacaattc cctgttaaaa
     4981 aaggccaata tcaaatccaa atagataaag attgtcatta ccacttagga aatgatacag
     5041 attatcttga atttggttat gacagtaatg tgcagcaagt ttacattgat cgtagccatc
     5101 ttattcaaaa aattctaggt gaagaagaac aggacactag tcgacggtat gtagatattg
     5161 aagctaaaga attggaagtt gttctagata aaaattccat cgagattttt gtcaatcaag
     5221 gtgaagcaag cttgactgca acttattact taacggtgcc agctgagcta tcacgaattg
     5281 attaaaaatt aagttatttc tcctaaagaa aaagttctct ttctaaaata gtggaaagag
     5341 gactttttgt gttttgggta tataagctta gtttatggta tttgtaaaat tggtgttgga
     5401 ttatgattta aactagtttt ctaaagaatt tgaaaaaaat tttatttaag caaaaaaagc
     5461 cctatattat aagacttttc ctgttgtatt tatatgcccc ctacagggat cgaacctgtg
     5521 acccacggat taagagtccg ctgctctgcc agctgagcta aggaggcaaa gaaaaaagct
     5581 gtattggtgc cgaaacttca cggtttgtat tgaacccgcg caattaagca ggtgggcaac
     5641 tcgctctaac tgaagctgtt tccgtgtgag acggcctaca tgctgttaga agacttttgt
     5701 ttccctaata atacaaaaaa tagtcggtca acacttaagt gtgaagtcgt acaccacagc
     5761 gtttctatac ttatatgata ccactttttc aaaaaaaatc aagaggaaag tgcaattttt
     5821 tgaaaaggat ttcagatttt tcgcaaacga tcgatagctt cctttctttg agccaaagcc
     5881 cgttcatatt taccagtatc ttctgcttgg aaatagtgat gattacgaat tttttctggc
     5941 agatagtctt gcttgatcca atttccagga tagttgtgtg gatagagata gtcttgggca
     6001 ttccccagtt ccttgcttcc actgtagtgc ccatcacgca ggtgtcgcgg aataggcaag
     6061 tgccctgatg ttttgaggtc agcaagtgcc ttatccatag ctacataggc tgagttggat
     6121 tttggagaaa gggccaaatc aatcacgaca ttggcaatga gaatgcgggc ttctgggaac
     6181 ccaatcttct gggcagcatc cagagcagtc acggtatgaa tctgggcctc aggattggct
     6241 aagccgatat cttcataggc gataacagtc aagcgacgag cgagactagg cagatcccca
     6301 gcctcaatca agcgggcagt atagtggaga ctggcatcca catctgagcc acgaatagac
     6361 ttttgcaggg cagatagaac atcatagtga ccgtctccat ccttgtccat agtgatgtag
     6421 cttctctgaa gactattttc catgatgtct aaggtgatgt ggcgaatgcc ctcgtcattc
     6481 tcaggggtag agagaacagc caagtccagt gagttaaagg cagagcgaag gtctccgttt
     6541 gtagaggttg cgatgaaatc cagcgcatcc tcatctagtt ctattggaaa atcaaaacca
     6601 cgttcagggt tacttagagc tatctgcagg gcctctttga cgtcttggtt agacagaggt
     6661 tccaactcga aaatttgaac acggctacga atggcaggag tgacagagaa gaaaggattt
     6721 tcagtcgttg ctccaatcat gatgaccagt ccactttcca agagaggcaa gaggaagtct
     6781 tgcttggtct tatctagtcg atgaatttcg tctagcaata ggacgagacc accagaaaat
     6841 ttagcttctt ccgagatttc ttgcagtcgc tttttactat caactgtcgc attgaaagtt
     6901 cgaaaggcat acttggtcgt tccagcgatg gcagaggcaa tactggtttt gccgattccc
     6961 ggagggccat atagaatcat ggaggacagg cggttggctt ccaccatgcg gcggatgatt
     7021 tttccaggtc cgaccagatg ctcctgaccg atgacctggt cgatggtttt agggcgcatg
     7081 cgaagcgcga gattgtctgg catagcagtc ctttctaacg tggatttgag tagaatgact
     7141 catctactat atcttactat gtccattcta gcctattagt tgtgatattt caatggtttt
     7201 gtaataatca gggagtgaga tagaatcttt tttactattt aatttaatga ggagaggtat
     7261 cagcatgaga gatttgattt tccttaaagc tcaaaatagg atagttgagc ctaaagaagt
     7321 ggaagtgctg acttgagaaa taatattgct gaatgtcatt gtatgaacta taatgaaaag
     7381 agaggtgata agcaatggaa atgatggacc aagtcaggaa attttgattt ttacaaaaca
     7441 aattcgtcat tggattctta gtgatcaagt tatttcaggg aaaagaaaac ttttcttccg
     7501 ggaagatact ccaaaagaga ttttagattt gtacgaaaac attaaatcta aacttgattt
     7561 tgcttaccaa gaagttcatt ctaataatgg gttgaaaaaa tatgaaaaat agaataattg
     7621 atgttttgaa gtagtaaatc gcctccttgt actaactgtt gaaaatcctg atttcgaaga
     7681 cttgagagtt aat
//
LOCUS       DBERDK010000084         7374 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.084, whole genome shotgun sequence.
ACCESSION   DBERDK010000084 DBERDK010000000
VERSION     DBERDK010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 7374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7374
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.084"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..4221
                     /locus_tag="ACL73L_001841"
     CDS             <1..4221
                     /locus_tag="ACL73L_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HGS0772765.1"
                     /translation="RYVVHIPKGWTNESKQTTNEWLFNFNQEGTVWIWMPKFEISDVD
                     TSYSEAPEDIEGQISTVESTFKQRANSLEAGVNRLTEGLRTKVDISALNVTAENIRQS
                     VKSLETDTQNKLNQKLSQAEFEVRAGSIRQEILNATKDKASKSELTQTAEELSSKIAS
                     VQVGGINLLRNTASLLIGDRSKGCWMSASGGNGRAISVEVLDPPKKMIKNMIRVIENT
                     NGGNKDLTQLVRLRIGEKYTISCYARIASDSPNANVNLLFRSWANNTDLNRKFQKSIS
                     HKNWQKYSFTFTADAIENSIQFGQSGAGIIEICAPKIESGTLATDYSEAPEDIEGQIS
                     TVESTFKQRANSLDAGVSRLTEGLRTKVDISALNVTAENIRQSVKSLETDTQNKLNQK
                     LSQAEFEVRAGSIRQEILNATKDKADKTLVVSEAGKLREEFSKMKVGGRNLWIKSKTV
                     GAVIEKLPENHVTGQKECYRLENNSTLMFNLEPDFSSRLYRKVTFSAWIKYENVVQGR
                     NFWNVFNCFKHYLFRKNSETGVQSGPDYATLGMYKGSADWKYITFTYDYSEKTNFDQL
                     KTSLRFNLEGATSGTAWVTGIKVEIGSVATDWSPAPEDADGLITEAKATFERTAQGLR
                     TDLSAIQEYVNKDGQRQEALQRYTREESTRQAIAVRELVNRDFVGKATYQEDVKGINQ
                     RIEAVKTSANKDIASQIASYRQSVDGKFTDISSQITTYKQDVGGQISGLSNRLTSSEQ
                     GTTTQISNLSNRINSNKQGADNQISNLKTQVATNKDNAERQMGRISDQVSANKANADS
                     QFANVTNQLVRKVETTDFQRVKETSKLYERILGNTENGIADKVARMALTNQLFQVEVG
                     KVAKGGRNYIRNGQFKNGSKNWLEYQSVNFGLNFNYQHSQNPNNRNRPGLHFYHDSQD
                     VANFFGIQQSFAFDGIRGEKVSVSLLVSKDGSDSYSGLKVALHYIKNKNIIGQEWQNI
                     PSPQITSKYKRFTFTFTLSDDVENLNLMLFGEKGKTINLYVTDVQLERGSVATDYKEA
                     PEDTDEAIRSVQSQLTGSWAVQNINSAGDIISGINLGANGHNRFVGKLTHITGETLID
                     RAVIKSAMVDKLKTANFEAGSVTTTILEAEAVTAEKLKVDDALIRKLTAKDAFIDRLT
                     SKRIFSTKVESVISSSTFLEAYQGRIGGFTLGQFDQGGGRWISGVNQFSVGMGNGAGY
                     GVRTAFWANWGNNWNYAGPKAWNVNTDGKMYCRNEVGFYDQVDFSNSSRANFYGNTTF
                     SRSPVFSNGIELGSKDVLGDGWNPKGGRNAVVWWNQVGSGSVKYWMEQKSDRRLKENI
                     TDTAVKALDKINRLRMVAFDFIENKKHEEIGLIAQEAETIVPRIVSRDPENPDGYLHI
                     DYTALVPYLIKAIQELNQKIEKMEKTIA"
     gene            4218..4335
                     /locus_tag="ACL73L_001842"
                     /pseudo
     CDS             4218..4335
                     /locus_tag="ACL73L_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063632.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase"
     gene            4316..4519
                     /locus_tag="ACL73L_001843"
     CDS             4316..4519
                     /locus_tag="ACL73L_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772766.1"
                     /translation="MNTEQLNQALQMTISEMSTTSTNSMITSNILSIQLNEQREENQR
                     LQARVDELEALLDEQTKPADKGE"
     gene            4522..4872
                     /locus_tag="ACL73L_001844"
     CDS             4522..4872
                     /locus_tag="ACL73L_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772767.1"
                     /translation="MAETIQNTDNLLDLTKITEPFDLASALRYMKENGEFIRCKNVSD
                     DFYMYRDVQKRPVIVNGRRQFKDVETVWAFNQWGGTIATINVAVLLNHEFYIMKFDAE
                     GNPDWTDPTGEPKE"
     gene            4882..5298
                     /locus_tag="ACL73L_001845"
     CDS             4882..5298
                     /locus_tag="ACL73L_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HGS0772768.1"
                     /translation="MQIEFFNFLRSVVQTEDGLVLYALALIVSMEIIDFVTGTIAAII
                     NPDIEYKSKIGINGLLRKISGVLLLMILIPASVLLPEKTGFAFLYSIYLGYIAFTFQS
                     LIENYRKLKGNVTLFQPIVKVFQRLLEKDDDTKKGE"
     gene            5302..5634
                     /locus_tag="ACL73L_001846"
     CDS             5302..5634
                     /locus_tag="ACL73L_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HGS0772769.1"
                     /translation="MQQITEIITNGAISILVILAGVVVRAVKDYLVQKGGEKTIKIVE
                     ILAKNAVNAVEQVAAETGYKGDEKLAQARAKVRAELTKYNISMTDKDLDTFVESAVKQ
                     MNDAWKGR"
     gene            5638..6594
                     /gene="lytA"
                     /locus_tag="ACL73L_001847"
     CDS             5638..6594
                     /gene="lytA"
                     /locus_tag="ACL73L_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HGS0772770.1"
                     /translation="MDIDRNRLRTGLPQVGVQPYRQVHAHSTGNRNSTVQNEADYHYR
                     KDPELGFFSHVVGNGRVMQVGPVNNGSWDVGGGWNAESYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKQDIENGLSAATGWQKNGTGYWYVHSDGSYPKDKFEKINGTWYYFDGSGYMLSD
                     RWKKHTDGNWYYFDQSGEMATGWKKIAEKWYYFDVEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPEFTVEPDGLITVK"
     gene            complement(6732..6911)
                     /locus_tag="ACL73L_001848"
     CDS             complement(6732..6911)
                     /locus_tag="ACL73L_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772771.1"
                     /translation="MREVIQELLDSSMSTSAISQGAGVPWTTVSDLRKGKTSMDKMAL
                     LTAEKLYEFATTDKQ"
     gene            complement(7053..7202)
                     /locus_tag="ACL73L_001849"
     CDS             complement(7053..7202)
                     /locus_tag="ACL73L_001849"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772772.1"
                     /translation="MNKEDWLRLLEKAIDNIPETVTAIASLVTAITVARQNKKRKPKS
                     RQRKR"
BASE COUNT         2365 a         1369 c         1687 g         1953 t
ORIGIN      
        1 cgatatgttg tccatattcc aaaaggttgg acaaacgaat ccaagcagac cacaaatgaa
       61 tggttgttca atttcaacca ggaaggaacc gtttggattt ggatgccgaa gtttgaaata
      121 agcgatgtag atacttctta ttcagaagct cctgaagata tagaaggtca gatttcaaca
      181 gttgaatcga cctttaaaca acgagccaac tcgctcgaag ctggtgtgaa tcgtctgact
      241 gaaggcctta gaaccaaagt ggatatcagc gcactcaacg tgactgctga aaatatccgg
      301 caatctgtga agagtcttga gacagacacg cagaacaagc taaatcagaa gttgagtcag
      361 gctgaatttg aggtgcgagc cggctctatc cgtcaggaaa tcctgaacgc aaccaaggat
      421 aaagccagca agtcagaact tacgcagaca gctgaggagc tctctagtaa gatagcgagt
      481 gtgcaggtcg gaggaatcaa cctcttgcgt aatactgcga gtttgttgat tggcgatcgt
      541 tcaaaaggat gttggatgag tgcaagcgga ggaaatgggc gagcgattag cgtagaagtt
      601 ttggatcctc ccaaaaaaat gataaaaaac atgattcgtg ttattgaaaa tacgaatggt
      661 ggaaataaag atttaactca acttgttaga ttgcgaattg gtgaaaagta cacgatttct
      721 tgttatgcaa ggattgctag cgatagccca aatgcaaacg tgaacttgct atttcgttcg
      781 tgggcaaata ataccgattt aaatcgcaaa tttcagaaat ccatctctca taaaaattgg
      841 caaaaatatt catttacatt tactgctgat gcaattgaaa attcaattca atttggacaa
      901 tctggcgcag gaattatcga aatatgcgct ccgaaaatcg aatcaggaac gttagcgact
      961 gattacagcg aagctcctga agatatagaa ggtcagattt caacagttga atcgaccttc
     1021 aaacaacgag ccaactcact cgacgctggt gtaagccgtc tgactgaagg ccttagaact
     1081 aaagtggata tcagcgcact caacgtgact gctgagaata ttaggcagtc ggtgaagagt
     1141 cttgagacag acacgcagaa caagctaaat cagaagttga gtcaggctga atttgaggtg
     1201 cgagctggct ctatccgtca ggaaatcctg aacgcaacca aggataaagc agataagact
     1261 ttagttgtat ctgaagccgg gaaattgcgt gaagaatttt caaaaatgaa ggtgggaggc
     1321 cggaatctat ggataaaatc caagacggtt ggagctgtaa ttgaaaaatt acctgaaaac
     1381 cacgtcacag gtcaaaaaga atgctatagg ctagagaaca actccacttt aatgttcaac
     1441 cttgaaccag atttcagctc aaggttgtat cgaaaagtta cttttagcgc ttggatcaag
     1501 tacgaaaatg tagtccaagg tcgaaatttt tggaatgtat ttaattgctt caaacattat
     1561 ctttttagaa aaaatagtga gaccggagta cagagtggtc cagattatgc tacgcttggt
     1621 atgtataaag gttcggcaga ttggaaatat attacattca cttatgacta ctctgaaaaa
     1681 acaaattttg atcaattgaa gacatcattg cgattcaatc ttgaaggtgc tacaagcggt
     1741 acagcttggg taacaggaat caaggttgaa atcggtagtg tggcgacgga ctggagtcct
     1801 gcgcctgagg acgctgatgg tctcatcact gaagctaagg ctacctttga gcggacagct
     1861 cagggcttgc gaaccgattt atcagctatt caggaatatg taaataaaga cggtcagcga
     1921 caggaagccc tacagcgcta tactcgtgag gagagcacga gacaagcgat agcagtccgt
     1981 gagctggtca atcgtgattt cgttggtaag gctacttatc aagaagatgt taagggtatc
     2041 aatcaaagga ttgaagctgt taaaactagt gcgaataaag acatcgctag tcaaatcgct
     2101 agctatcgtc aatctgtaga tggtaagttc acggatattt caagtcagat aactacttat
     2161 aagcaagatg tgggcggtca aatcagtggc ctttcaaata gacttacaag cagtgagcaa
     2221 ggaaccacta ctcagatttc aaatctttca aatcggataa acagtaataa acaaggcgca
     2281 gataatcaga tttcaaattt aaagactcag gtcgctacaa acaaggataa tgctgaacga
     2341 caaatgggta gaatatctga tcaggtttct gcaaacaaag cgaatgctga tagtcaattt
     2401 gcgaatgtga ccaatcaact agtgcgaaaa gtagagacta ctgacttcca gcgtgttaag
     2461 gaaaccagta aactttacga gcggattttg ggcaatactg aaaatggaat tgcggataag
     2521 gttgctcgca tggctctgac caatcaactg tttcaggttg aggttgggaa ggttgccaaa
     2581 ggtggtcgaa attatatcag aaatggtcaa tttaagaacg gttcgaaaaa ctggcttgaa
     2641 tatcaatctg ttaattttgg tttgaatttc aactatcaac actctcaaaa tcctaataat
     2701 cgaaatcgtc caggactaca cttctatcac gattctcaag atgttgctaa tttttttgga
     2761 attcagcaat cttttgcatt tgatggtatt cgaggggaaa aggtgagtgt atctcttctt
     2821 gtttcaaagg atggcagtga tagctacagc ggtttgaaag ttgcattgca ctacatcaaa
     2881 aacaaaaaca ttattgggca agagtggcaa aatatcccaa gtccacaaat aacatcgaag
     2941 tataagcgtt tcacatttac gtttacttta tcagacgatg tagagaatct gaatttgatg
     3001 ttgtttggtg aaaaagggaa gaccatcaac ctctatgtta cagatgttca actcgaaaga
     3061 ggttctgtcg cgacggatta caaagaagct cctgaagaca cggacgaagc gattcgctct
     3121 gttcaaagcc aactaactgg ctcatgggca gttcaaaaca tcaactcggc tggagatatc
     3181 atctctggaa tcaatcttgg cgccaatgga cataaccgct ttgttgggaa attgacccac
     3241 atcactggag agaccctgat tgacagagca gtcatcaagt ctgccatggt tgataagctc
     3301 aaaacggcca attttgaagc tggttcggtc acgactacga tattagaagc tgaagcggta
     3361 actgctgaga agttgaaagt tgacgatgcg cttattagaa aattaactgc aaaagatgct
     3421 tttattgacc gactgacatc taaacgtatc ttctctacta aggttgagtc cgtcatttct
     3481 agctcaacct tcctagaagc ctatcaaggc cgaatcggtg gattcacact tggtcaattt
     3541 gaccagggtg gcggtcgctg gatttcaggt gtcaatcagt tctctgttgg tatggggaat
     3601 ggtgccgggt atggagtccg gacagccttc tgggcgaact ggggaaataa ttggaactat
     3661 gccggaccta aagcatggaa cgtcaatact gatgggaaaa tgtactgtag gaatgaagtc
     3721 ggtttttatg atcaagtgga tttttcgaat tcatcgagag caaacttcta tgggaatact
     3781 actttttctc gttctcctgt gttttcaaat ggtatcgaac ttggaagtaa agatgtgctt
     3841 ggtgatggtt ggaatcccaa aggcggaagg aatgcggttg tttggtggaa tcaggtcggt
     3901 agcggtagcg tgaagtattg gatggaacaa aaatcagaca gacgcttaaa agagaacatc
     3961 acagatacag ctgtgaaagc cttggataaa atcaacagat taagaatggt tgcatttgat
     4021 ttcatcgaaa ataagaaaca tgaggagatt ggtctaatag ctcaagaggc tgaaaccatc
     4081 gttccaagaa ttgtctcacg agatcctgag aatccagatg gctatctgca tattgactat
     4141 accgctttag ttccttactt aatcaaggct attcaagaat taaatcaaaa aatagaaaaa
     4201 atggagaaaa caatagcatg aataacaaca tgttgaccaa tatcgcactt taaagcaatt
     4261 caggagcttg ctcttgaaaa tagaaaacga acacacagat tggagaactt agaaaatgaa
     4321 cacagaacag cttaaccaag ccttacaaat gacaattagt gaaatgtcaa caacttcaac
     4381 aaattcgatg attacaagta atatcttgag tattcagttg aatgagcaaa gggaagagaa
     4441 tcaaagactt caagcacgag tggatgagct ggaagctctg cttgatgaac aaactaaacc
     4501 agcagacaaa ggagaataga catggcagaa acaattcaaa acacagataa cttactagac
     4561 cttacaaaaa tcacagaacc atttgatctt gcgagtgctt tgcgctacat gaaagaaaat
     4621 ggagagttca ttcgttgcaa gaatgtaagc gatgacttct atatgtatcg tgacgttcaa
     4681 aaacgtcctg tgatcgtaaa tggccgtcgc caattcaagg atgttgaaac cgtttgggcg
     4741 ttcaatcagt ggggtggtac aatcgcaaca atcaacgtag ccgttctgtt gaatcatgaa
     4801 ttctatatca tgaaatttga tgcagagggc aatcctgact ggacggatcc aacgggagaa
     4861 cctaaagaat aggagatgtg tatgcaaatt gaatttttca attttctaag aagtgtcgta
     4921 cagactgaag atggtttggt cttatacgct ctagcactga ttgtctcaat ggaaatcatt
     4981 gattttgtga cagggacgat tgcggcgatt atcaatcctg acatcgagta caagagcaaa
     5041 atcggcatta acgggctcct tcgtaagatt tcaggggttc tcttactgat gatcctcatt
     5101 ccggcgtccg ttttgttgcc tgaaaagaca ggttttgcat tcttgtactc aatctatctc
     5161 gggtacatcg catttacttt tcaatcactc attgaaaatt accgcaaatt aaaaggaaat
     5221 gttactcttt ttcagccgat tgtaaaagta tttcagcgat tacttgaaaa agatgatgat
     5281 acgaaaaaag gagaataaca aatgcaacaa attactgaaa tcattactaa tggagcaatc
     5341 agcatcctag tcattttagc aggggtggta gttagggcag tcaaggacta cctggttcaa
     5401 aaaggtggag aaaagaccat caaaatcgtt gaaatcttgg ccaagaacgc agttaatgcc
     5461 gtggagcagg tagctgctga aactggctac aagggagatg aaaaactggc acaggctcgc
     5521 gctaaagtcc gtgctgagct tacaaaatac aatattagca tgactgacaa agacttagac
     5581 accttcgtag agtcagcagt gaagcagatg aatgacgcat ggaaaggacg atagggaatg
     5641 gatatcgata gaaacagact acgtacaggc ttgccacagg ttggggtgca gccttatcga
     5701 caagtacatg ctcactcaac aggtaaccgc aactcaaccg tacagaatga agcggattat
     5761 cactatagaa aggaccctga actagggttc ttttcacatg ttgtcggaaa cggccgtgtc
     5821 atgcaagtag gacctgtgaa caacggaagt tgggatgttg ggggcggttg gaatgctgag
     5881 agttacgcag cggttgaact gattgaaagc cattcaacta aggaagagtt tatgacggac
     5941 taccgcctct atatcgaatt gctacgcaat ctagcggacg aagcaggttt gccgaaaacg
     6001 cttgatacag ggagtttagc tggaattaaa acgcacgagt attgcacgaa taaccaacca
     6061 aacaaccact cagaccatgt ggatccatat ccatatcttg ctaaatgggg cattagccgt
     6121 gaacagttta agcaagacat cgaaaacggc ttgagcgctg caacaggctg gcagaaaaat
     6181 ggcactggct actggtacgt acactcagac ggctcttatc caaaagataa gtttgagaaa
     6241 atcaacggta cctggtatta tttcgatggc tcaggctata tgctttcaga ccgctggaag
     6301 aagcacacag acggtaattg gtactacttt gaccaatcag gcgaaatggc tacaggctgg
     6361 aagaaaatcg ctgagaagtg gtactatttt gatgtagaag gtgccatgaa gacaggctgg
     6421 gtcaagtaca aggacacttg gtactactta gacgctaaag aaggcgccat ggtatcaaat
     6481 gcctttatcc agtcagcgga cggaacaggc tggtactacc tcaaaccaga cggaacactg
     6541 gcagacaagc cagagttcac agtagagcca gatggcttga ttacagttaa ataaatagaa
     6601 aggaaacttt ctaaattgtt ctttcaccgc aggcttaggc ttgcggtttt ttatttgctc
     6661 tgattcttta aaaaagcgtt ttcttgaaga atagagaggt gttttgtcaa aaataaaaac
     6721 agtgaccgaa atcactgctt atcagttgta gcaaattcat aaagtttttc tgctgtgaga
     6781 agtgccattt tgtccatgct tgtttttcct tttctgaggt cagaaacagt agtccatgga
     6841 actccagcac cttgggaaat agcagatgta gacatcgaac tgtctaacaa ttcttgaata
     6901 acttctctca tcttatttgt cctttttatt ttttagataa atatatacat tgattgcaat
     6961 tataaaaata gctattgcac taaccattgc atttcctctt tccatttgat aaaatagagg
     7021 tgtaaggggc tttcgcccca acctcttagc gtttaccttt ttctttgacg ggatttcggt
     7081 ttacgctttt tgttttgcct tgcgaccgtt attgcggtca ctagacttgc gatagcagtt
     7141 accgtttcag gaatattgtc tattgccttt tcaagtaacc tgagccaatc ttctttgttc
     7201 aacttcatca ccccctttcc ttatcttgat tatattatat cacggtacac cgagaaagtc
     7261 aagcgttttg atgaagtttt tttaattttt tcaaaaaaaa tagaccttgt ccagaggtcg
     7321 gggagttgga ggggacaccc tccaagagtg ttgatttaat aagattttat ttta
//
LOCUS       DBERDK010000085         7070 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.085, whole genome shotgun sequence.
ACCESSION   DBERDK010000085 DBERDK010000000
VERSION     DBERDK010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 7070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7070
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.085"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..709
                     /locus_tag="ACL73L_001850"
     CDS             <1..709
                     /locus_tag="ACL73L_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772773.1"
                     /translation="FLKENIRNLYSSGVIYLSLLISFIPPILLTFFILKTQETSLGIK
                     HISNFYAMLGMLMAVIHANRVISRDFSHNTVSLFYNQQKNRMIYVLSNFLYAISVSII
                     YALNGIVLLVIVSKLGIPGDLGLDFIVAIVVNTILLVLFYFLLSYIFYLYKLKSGLVF
                     GILVALLLFIPNILNTIMMNTSNDLFIKAIELLPFYSLPLFVVSNTMSISQYLVLITT
                     IILLYFFTLKKSKKYSF"
     gene            1095..1304
                     /locus_tag="ACL73L_001851"
     CDS             1095..1304
                     /locus_tag="ACL73L_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGS0772774.1"
                     /translation="MAKNLKLKLARVERDLTQGQLAEAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
     gene            1294..1782
                     /locus_tag="ACL73L_001852"
     CDS             1294..1782
                     /locus_tag="ACL73L_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6773 family protein"
                     /protein_id="HGS0772775.1"
                     /translation="MKNRFYYYQLLDEREEQLFNKAGSESFYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGVTYYSRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAWVITYVIYLPWVFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            1898..3358
                     /locus_tag="ACL73L_001853"
     CDS             1898..3358
                     /locus_tag="ACL73L_001853"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HGS0772776.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIHNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEDLRFSDSDIAYLESLGYHGAFLDYLCNFKLELTVRSAQEGD
                     LVFANEPIVQVEGPLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWGTRAAVIGGANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYA
                     ATHKNCVFLVDTYDTLRIGVPAAIQVARELGDQINFMGVRIDSGDIAYISKKVRQQLD
                     EAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIE
                     DETGQMRNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISDMTEIKM
                     FHPTYTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRV
                     LNPQHYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            3355..4179
                     /gene="nadE"
                     /locus_tag="ACL73L_001854"
     CDS             3355..4179
                     /gene="nadE"
                     /locus_tag="ACL73L_001854"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HGS0772777.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKNFV
                     LGISGGQDSTLAGRLAQLAIEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            4477..5028
                     /locus_tag="ACL73L_001855"
     CDS             4477..5028
                     /locus_tag="ACL73L_001855"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS0772778.1"
                     /translation="MKSIGTQILQTERLILRRFVESDAEAMFQNWASSAENLTYVTWD
                     PHPDVEITRNSIRNWVASYTNLNYYKWAICLKENPEQVIGDISIVKIDEADLSCEIGY
                     VLGKVYWGHGMMTEALKAVLDFCFTQAGFQKVRARYASLNPASGRVMEKAGMSYLQTI
                     VNGVERKGYLADLIYYGISREEC"
     gene            6072..6434
                     /locus_tag="ACL73L_001856"
     CDS             6072..6434
                     /locus_tag="ACL73L_001856"
                     /inference="COORDINATES: protein motif:HMM:NF019142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HGS0772779.1"
                     /translation="MKVPAKSKVEEVLVPFATKYEADNDLSAGQEQEITLGKNGKTVT
                     TITYDVDGKSGQVTESTLSQKEDSQTRVVKKGTKPQVLVQEISIETEYLDGPTLDKSQ
                     EVEEVGEIGKLLLLQSIL"
     gene            6488..6877
                     /locus_tag="ACL73L_001857"
     CDS             6488..6877
                     /locus_tag="ACL73L_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HGS0772780.1"
                     /translation="MVKRRIRRGTREPEKVVVPEQSSIPSYPVSVTSNQGTDVAVEPA
                     KAVAPTTGWKQENGMWYFYNTDGSMATGWVQVNGSWYYLNSNGSMKVNQWFQVGGKWY
                     YVNTSGELAVNTSIDGYRVNDNGEWVR"
BASE COUNT         2229 a         1194 c         1517 g         2130 t
ORIGIN      
        1 ttttcttaag gaaaatataa gaaatttata ttcatcaggc gtaatatatc tcagtttgct
       61 tatctcgttt ataccgccga tattgcttac attctttatt ctaaagactc aagagacatc
      121 gcttggtatt aagcatattt caaactttta tgctatgctc ggtatgttaa tggctgttat
      181 acatgctaat cgagttatta gtagagattt ttcccacaat acggtaagtt tgttttataa
      241 tcaacagaag aatcggatga tttacgtctt gtctaatttt ctatatgcca tctcagtttc
      301 cattatttat gctttgaatg gcattgtgtt actagtcata gtaagtaagt tgggtattcc
      361 aggtgattta ggattagatt ttatagtagc tattgtagtc aatacaattt tgttagtcct
      421 gttttatttt ctattatctt acattttcta tttatacaaa ttgaaaagtg gcttggtatt
      481 tggtatttta gtagctttac tactctttat ccctaatata ttaaatacga ttatgatgaa
      541 tactagtaat gatttgttta tcaaagcaat tgaacttctt cctttttatt ctttacctct
      601 atttgtggtt tcaaatacga tgtctattag tcagtatctt gtgctaatca ctacaatcat
      661 tttattgtac tttttcactc tcaagaaaag caagaagtat tcattttagt ttcatttagt
      721 attattttgc atacctaaaa tacagtaaaa aaatcagtca tcttggtatg ctcctgcttt
      781 cactattcaa cacgtttttg acttatacta ggctcatttc caaaagcatt atataatagt
      841 gatatgaaac caactaaact aaacaagaaa tataagcaat aaaaactcgt ttaaaagatt
      901 ctactaaagc taatactaaa taaaaataaa agagtaaact aggaaattta tttcaaacaa
      961 cctaaaatac tgattttcgt ctgaagataa tactggagtg caaattaatg gggttataat
     1021 aaatagctga tagcttgtgt tggttttgga ttttttaaga gtagatgagt attaaaacta
     1081 taaggaggac gaaggtggct aaaaatttaa aattaaaatt agctcgggta gagcgtgatt
     1141 taacacaagg tcaactggca gaggctgtcg gggtgacacg ccagactatt ggtttgatag
     1201 aggcgggaaa atacaatccc agtctctcgc tctgccagtc tatttgcaga tgtttaggga
     1261 aaaccctaga ccaactattt tgggaggaag aagatgaaaa atagatttta ttattatcaa
     1321 ttactagacg aaagagaaga acaactgttc aataaagcgg gatctgaaag tttctatatc
     1381 tgcattgctt tgtcgctcct atcttatatc atttcagtat tagcaccaag cctttttaat
     1441 tctaatatgc tgctaatcgt tatcatcata gggacatttt actttttcaa tcgcgcccgt
     1501 tatctgggag tgacctacta tagtcgtttt cattttacga ttttgggttg ttttttccta
     1561 accttggcta ttacggctct tttgatgttg cagaattatc aattcaacat agaaatttat
     1621 cagcacaatc ctttgaattt taaatacctg tctgcttggg tcattactta tgtcatttac
     1681 cttccgtggg tctttattgg caatcttggt cttaagagct atggcgaatg ggctcagaaa
     1741 aaatttgaac aagatatgga tgaattggag agtggagaat agcttgttac tcttttctca
     1801 atccagctaa aatgtgatat aatagtacta atttattgga atacatgaaa gttcttgaaa
     1861 attttcatgg gtttctagct aaggaagtag gaaaagtatg tatccagatg atagtttgac
     1921 attgcacacg gacttgtacc agatcaacat gatgcaggtt tactttgacc aagggattca
     1981 caataagaag gcggtctttg aggtgtattt ccgccaacag ccttttaaga acggctatgc
     2041 ggtttttgca ggtttggaaa gaattgtgaa ctatcttgaa gacttgcgtt tttcagatag
     2101 tgatatagcc tatttggagt cgcttggtta tcatggggcg ttcttggatt acctttgcaa
     2161 tttcaagttg gagttgaccg ttcgttctgc ccaagaaggg gatttggttt ttgctaatga
     2221 accgattgtg caggtggaag gacctctagc ccaatgtcag ttggtcgaaa cggctctttt
     2281 gaacatcgtc aactaccaga ccttggtggc gacgaaggca gctcgtattc gttcggttat
     2341 cgaagatgaa cccttgatgg agtttgggac acgtcgggct caagaaatgg atgcggccat
     2401 ctggggaaca cgtgcagcgg tgattggtgg cgccaatgga accagcaacg tgcgtgcggg
     2461 taaactcttt gacattcctg ttttgggaac ccatgcccat gccttggtgc aggtttatgg
     2521 caatgactat gaggctttca aggcttacgc tgcgacccac aaaaattgtg tctttcttgt
     2581 ggatacctat gatacccttc gcatcggtgt accagctgcc attcaggtgg cgcgtgaact
     2641 gggtgatcag attaacttta tgggtgtgcg gattgactct ggggatattg cttacatttc
     2701 taagaaagtc cgtcagcaac tggacgaggc tggatttaca gaggctaaga tttatgcttc
     2761 taatgatcta gatgaaaata ccatccttaa cctcaagatg caaaaggcca agattgatgt
     2821 ctggggtgtg ggtaccaagc tgattacagc ctatgaccag ccggctcttg gggcggttta
     2881 caagattgtt gcaattgaag atgaaactgg tcagatgcgc aatacgatta agctgtctaa
     2941 taatgcggaa aaagtgtcga cgccaggtaa gaagcaggtg tggcgcatta ccagtcgtga
     3001 aaaaggcaag tcagaaggtg actacatcac ttatgatggt gtggatatta gcgacatgac
     3061 agaaatcaag atgttccatc cgacctatac atacatcaag aagacggttc gtaattttga
     3121 tgccgttcct ctcttggtgg atatcttcaa agaaggaata ttagtttaca acttgcctag
     3181 tttgactgac attcaggatt atgcccgtaa agaatttgac aagttgtggg atgagtataa
     3241 gcgtgtgctc aatccgcagc actatccagt ggatttggca cgtgatgtat ggcaagataa
     3301 gatggacttg attgataaga tgcgcaagga agcccttggt gaaggagaag aagaatgagt
     3361 ttgcaagaaa cgattatcca agagctgggt gtcaaaccag tgattgatgc ccaggaagaa
     3421 atccgtcgtt ctattgattt cttaaaaaga tatctgaaaa aacatccctt cctaaaaaac
     3481 tttgtactag ggatttctgg gggacaagac tcaaccttgg caggacgttt ggcgcaatta
     3541 gctatagaag aactgcgagc tgaaacggga gacgacagct acaaatttat cgctgtccgc
     3601 ctgccatacg gagtgcaagc tgatgaagca gatgctcaaa aagccctagc cttcatccag
     3661 ccagatgtca gcttggttgt gaatatcaag gagtcagctg atgctatgac agctgcagtt
     3721 gaagcgacag gtagtcctgt ttcagacttc aacaagggga atatcaaggc acgttgccgt
     3781 atgattgctc agtatgccct tgctggttcc catagcggag cggtcattgg aacagaccac
     3841 gccgcggaaa atatcacagg tttctttacc aagtttggtg acggcggtgc ggatattctc
     3901 cctctttacc gcctcaataa acgccaagga aaacagctct tgcagaaact tggcgcagag
     3961 ccagcccttt atgaaaaaat cccaacagca gacctagaag aagataaacc aggcctagct
     4021 gacgaagtcg cacttggagt cacctacgca gagattgacg actacctaga aggcaaaaca
     4081 atcagcccag aagctcaagc gaccattgaa aactggtggc acaaaggcca acacaaacgc
     4141 cacttaccca tcaccgtatt tgatgacttt tgggagtaaa aacctccatt ggaggttttc
     4201 agcctctcat cttgaaataa gaaagtgaga gaaggtctgg gggatcttga accccgagtt
     4261 tagaaataag aaaatgaggc agattcggta actcgaagag ttcgatttca tcgtcttacc
     4321 cctgcaacga tgactaggtt tgaaaaagct tgctagagcg catttcaaac caggcagcga
     4381 ctgcgtcaag aaattagaag acaaactcgt tttctagctg ttactgagtt gagccttttt
     4441 actacgagca tagaaataag gaagtgaggt agcatcatga aatctatcgg tacgcaaata
     4501 ttacagacag aacgtttgat tttaagaaga tttgtggaga gtgatgcaga agctatgttt
     4561 caaaattggg cttcatccgc tgagaatttg acctatgtta cctgggatcc ccatcctgat
     4621 gtcgaaatca ctcgaaactc gattcgcaat tgggttgctt cctatactaa tctcaactat
     4681 tacaaatggg ccatttgtct aaaagaaaac ccagagcaag tgataggaga tatcagcatc
     4741 gttaagatag acgaggctga tttaagctgt gaaatcggct atgtgctagg caaggtttac
     4801 tggggacatg gtatgatgac agaggccttg aaagctgtct tggacttttg ttttactcaa
     4861 gcaggttttc aaaaggtcag agcacgttat gccagtctca acccagcttc aggtcgtgtc
     4921 atggaaaagg ctggaatgtc ctatctacaa accattgtta atggtgtaga gagaaaaggc
     4981 tatcttgcgg atcttattta ttatggtata agtagggaag aatgttgaat tctattttct
     5041 gtttctatcg aagtcaacta tttattgtaa atataataat tagcattcca agtttatttg
     5101 caattttaaa atagcatatt gattattaca agacagatgc tctagttcct tctttaatct
     5161 ggtttagtat tagttaaaaa atcgcttgaa gcttgtaact aagagggagc taatcgacta
     5221 ggttctccag ccgaacaggt ggtaatgtac tttttatagt gtaatcctag ctgttgttaa
     5281 atttaaaata gaatcctcta tcgagttagg gaattaaatt caaccaattt tatttatgtt
     5341 tttctatcaa attatctaat attaaaatag tctcattctg atgagaaaac tattctattt
     5401 atactgcatc gttgtgtaag gaatgatgaa agatgagtga aaattatcaa gttggaatgt
     5461 ttgtatctaa atatattagc atgtatttag atcagatgtc tgcaatcctt tatatatgag
     5521 attgattacg atatagttaa agtgttagtt caatagtaaa tatatcattc taagtctatt
     5581 ttatatataa agtatattgt ttaacgtttg atagaaaaga tgctatacta gaagtaggat
     5641 gtaatggcaa aatctaatac taataagcgc tatgcaatac gtaaactcgg aaagattttt
     5701 ggttctgcgg taatcgggct aactttattt ttaggaggaa tctgaataca agttttgctc
     5761 atgctgctga tgggattcaa tatgtcagag atgatactag agataaagaa gagggaatag
     5821 agtatgatga cgctgacaat ggggatatta ttgtaaaagt agcgactaaa cctaaggtag
     5881 taaccaagaa aatttcaagt acgcgaattc gttttgaaaa agatgaaaca aaagaccgta
     5941 gtgaaaatca tgttacaatt gacggagagg atggctatgt aaccacgaca aggacctacg
     6001 atgttaatcc agagactggt catgttaccg aaaaggttac tgttgataga aaagaagcca
     6061 cggatacagt tatcaaagtt ccagctaaaa gcaaggttga agaagttctt gttccatttg
     6121 ctactaaata tgaagcagac aatgaccttt ctgcaggaca ggagcaagag attactctag
     6181 gaaagaatgg gaaaacagtt acaacgataa cttatgatgt agatggaaag agtggacaag
     6241 taactgagag tactttaagt caaaaagaag actctcaaac aagagttgtt aaaaaaggaa
     6301 ccaagcccca agttcttgtc caagaaattt caatcgaaac agaatatctc gatggcccaa
     6361 ctcttgataa aagtcaagaa gtagaagaag taggagaaat tggtaaatta ctcttactac
     6421 aatctatact gtagatgaac gtgatggaac aattgaagaa actacttctc gtcaaattac
     6481 taaagagatg gtaaaaagac gtataaggag agggacgaga gaacctgaaa aagttgttgt
     6541 tcctgagcaa tcatctattc cttcgtatcc tgtatctgtt acatctaacc aaggaacaga
     6601 tgtagcagta gaaccagcta aagcagttgc tccaacaaca ggctggaaac aagaaaatgg
     6661 tatgtggtat ttttataata ctgatggttc catggcaaca ggttgggtac aagttaatgg
     6721 ttcatggtac tacctcaaca gcaacggttc tatgaaagtc aatcaatggt tccaagttgg
     6781 tggtaaatgg tattatgtaa atacatcggg tgagttagcg gtcaatacaa gtatagatgg
     6841 ctatagagtc aatgataatg gtgaatgggt gcgttaacag attcctccct gcgacaaaat
     6901 tgataatagt atgtctgaat atataattat atataaaaag ctatagataa ggtcatctta
     6961 tagcgactat aaaaaagtga acaagcctag tcttctgtac attagaattc agatttgttc
     7021 actttttgtt taatactcaa tgaaaatcaa agagcaaact agaaaactag
//
LOCUS       DBERDK010000086         6980 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.086, whole genome shotgun sequence.
ACCESSION   DBERDK010000086 DBERDK010000000
VERSION     DBERDK010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6980
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.086"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(121..396)
                     /locus_tag="ACL73L_001858"
     CDS             complement(121..396)
                     /locus_tag="ACL73L_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HGS0772781.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVEAVFAAVADYLAAGEKVQ
                     LIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAFKAGKALKDAVK"
     gene            complement(503..1342)
                     /locus_tag="ACL73L_001859"
     CDS             complement(503..1342)
                     /locus_tag="ACL73L_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HGS0772782.1"
                     /translation="MTKIKIVTDSSVTIEPELVKQLDITIVPLSVMIDNVVYSDADLK
                     EEGKFLQLMQESKNLPKTSQPPVGVFAEIFEDLCKDGGQILAIHMSHALSGTVEAARQ
                     GASLSTADVTVVDSSFTDQALKFQVVEAAKLAQEGKDMEAILSHVEEVKNHTELYIGV
                     STLENLVKGGRISRVTGLLSSLLNIRVVMQMKDHELQPMVKGRGTKTFKKWLDELITS
                     LSERAVAEIGISYSGSDDWAKEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIR
                     YHS"
     gene            1484..2332
                     /locus_tag="ACL73L_001860"
     CDS             1484..2332
                     /locus_tag="ACL73L_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HGS0772783.1"
                     /translation="MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITF
                     YLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSAWLAFFEHIPLHIDLQ
                     GDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCIL
                     MLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDL
                     GRGVTFISGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEG
                     FTFEKE"
     gene            complement(2425..3342)
                     /locus_tag="ACL73L_001861"
     CDS             complement(2425..3342)
                     /locus_tag="ACL73L_001861"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HGS0772784.1"
                     /translation="MIITIPIKNQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAA
                     RKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQF
                     ASLTAQEFFATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKDYFDGEVIIVPPVEDEKG
                     KISSTRIRQAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRTIGYPTANLVLLDRTYM
                     PADGVYVVDVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLD
                     RIRDMTKFDSVDQLVDQLKADEEVTRNWS"
     gene            3821..3969
                     /locus_tag="ACL73L_001862"
                     /pseudo
     CDS             3821..3969
                     /locus_tag="ACL73L_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
     gene            complement(4295..4588)
                     /gene="rpmA"
                     /locus_tag="ACL73L_001863"
     CDS             complement(4295..4588)
                     /gene="rpmA"
                     /locus_tag="ACL73L_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HGS0772785.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(4605..4949)
                     /locus_tag="ACL73L_001864"
     CDS             complement(4605..4949)
                     /locus_tag="ACL73L_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HGS0772786.1"
                     /translation="MIQAVFERAEDGELRSAEITGHAESGEYGLDVVCASVSTLAINF
                     INSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENS
                     SEFVQTRVITEN"
     gene            complement(4965..5279)
                     /gene="rplU"
                     /locus_tag="ACL73L_001865"
     CDS             complement(4965..5279)
                     /gene="rplU"
                     /locus_tag="ACL73L_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HGS0772787.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAVYVEKLNVEAGQEVTFNEVVLVG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     misc_feature    complement(5315..5402)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            complement(5461..6165)
                     /locus_tag="ACL73L_001866"
     CDS             complement(5461..6165)
                     /locus_tag="ACL73L_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004241810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HGS0772788.1"
                     /translation="MGGKMRLLPIRKISRQSKRLALFLTFCAGYVDAYTFIVRGNTLV
                     AGQTGNVVFLSVELIKNNVSDVRDKVLTLLAFMMGVFLLTIYKEKLRIVKKPILSLIP
                     LAILSIIIAFVPQTVDNIYLVPPLAFCMGLVTTAFGEVSGIAYNNAFMTGNIKRTMLA
                     FGDYFRTKHTPFLREGFIFVSLLSSFVLGVVFSAYLTIFYHEKTILGVPIMMSVFYLS
                     MLFASWQKKVKEKASF"
     gene            6567..6866
                     /locus_tag="ACL73L_001867"
     CDS             6567..6866
                     /locus_tag="ACL73L_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HGS0772789.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQSQAELAAMQFVGKSAQDLVQATLT
                     GDKKVVSIDFNPAVVDPEDLETLSDMTVQAINSALEQIDETTKKKLGAFAGKLPF"
BASE COUNT         2198 a         1413 c         1162 g         2207 t
ORIGIN      
        1 tatatataca atattttcgg aacattcaac tttttaactc tatttattac tagatttcat
       61 aattaaaaag cctactgacc aagctagaaa gcttgataca ataggctttt taaagactga
      121 ttatttaaca gcgtctttaa gagctttacc agctttgaat gctggcactt tagaagctgc
      181 aattgtcatt tctttaccag tttgtgggtt gcgaccttta cgttctgcgc gctcacgaac
      241 ttcaaagtta ccaaaaccga tcaactgaac tttttcacca gctgcaagat agtcagctac
      301 tgctgcaaat acagcttcaa ctgctgctgc tgagtctttc ttagtcaatt ctgtagcttc
      361 tgctacttta gcgatcaaat cttgtttgtt tgccatgtta atgattcctc caaataattt
      421 ctaattaaca aatataatca tatcctaaaa tcccttttag gtcaagttaa aaacgctatt
      481 tcttcccatt ttatttattt ttttaggagt ggtaacgtat caaaatagcc caagcgttct
      541 cacccgtgtg agtttgaata atggaacccg tttccaaaac agaaattggc ttttcaacat
      601 aagcttgtaa gctttctttc atctcttttg cccaatcatc actaccagaa tatgaaattc
      661 caatctctgc tacagcacgt tcagaaagcg atgttatcaa ctcatctaac cattttttaa
      721 atgttttagt tccacgacct ttaaccattg gctgcaattc atggtctttc atttgcatga
      781 caacacggat attgagaaga gagctcaaca agccagttac acggctaatt cgtccacctt
      841 tgacaagatt ttccaaagtt gaaacaccaa tataaagctc tgtatggttt ttaacctctt
      901 ctacatgaga taaaattgcc tccatatctt taccttcttg agctaacttc gcagcctcaa
      961 caacttggaa tttcagggct tggtcagtga aggaactatc aacaacagtc acatctgcag
     1021 tagataggct agcaccttgg cgtgctgctt ctaccgtacc cgaaagagca tgggacatat
     1081 gaatagcaag aatctggcca ccatctttgc ataggtcttc aaaaatctca gcaaagacac
     1141 ctacaggtgg ctgacttgtt ttcggaagat tcttactttc ttgcatcaac tgaagaaatt
     1201 taccttcttc tttcaaatcc gcatcagaat aaacaacatt atcaatcatt acagataatg
     1261 gaacaattgt aatatctaat tgctttacta gttcaggttc aatagtaaca gatgaatcgg
     1321 ttacaatctt aatttttgtc atagtatcaa tctttctatt ttaggattca gattggtttc
     1381 cttacttcta attatatcaa aaaaaagatt aaaaatccta atggagtcaa tcaaattttc
     1441 cgtaaaattt gatataatca acttataaga aaagaggtgt cctatgatta aaaaaattta
     1501 ccccattttt accattttac taggtgctgc tatttatgct tttggactga cttattttgt
     1561 agttccccat catctctttg aaggaggggc gacaggcatt accctcatca ccttttatct
     1621 ttttaaaatc cctgtttccc tcatgaacct gctgattaat attccccttt tcatcctagc
     1681 ttggaagatc tttggagcca aatccctcta ttctagttta ctaggaacct tagctttgtc
     1741 cgcctggtta gctttttttg agcatattcc ccttcatatt gatcttcaag gtgatttact
     1801 aatcacagcc cttatagcgg gaatcctatt gggaattggc cttggaatta tttttaatgc
     1861 tggaggtaca actggcggaa ctgatattct agctcgtatt ctcaacaaat acactcatat
     1921 atccatagga aaactgctct ttatcttaga tttttgtatt ctcatgttga ttctcctaat
     1981 cttcaaggat ttgagattgg tttcctacac gcttttgttt gattttattg tttctcgtgt
     2041 tattgatttg attggtgaag gaggatatgc cggcaaaggc tttatgatta tcacaaaacg
     2101 tcctgaccaa cttgctaagg cgattaatga tgacctcgga agaggtgtta cttttatttc
     2161 tggtcaaggc tactatagta aagaaaattt gaaaatcatc tactgtattg tcggaagaaa
     2221 tgaaattgtg aaaacgaagg aaatgattca tcgaatcgat cctcaagcct ttataactat
     2281 tacagaagcc catgaaatcc taggagaagg cttcaccttt gaaaaagaat aaaaagaggt
     2341 aatgtcgtga cctcaaaagt tagactaaat catctatctt ttgggttaca gacaacctct
     2401 tttttgtttt atttactcaa gctcttaaga ccaattccga gttacttctt catcagcctt
     2461 taactgatcc actaattggt caactgagtc aaatttggtc atatcacgaa tgcgatcaag
     2521 ccaataaacc atgacggttt ccccataaat atcttgatta aaatcaaaaa tattgacttc
     2581 aaaacgtgct tcttctccat caaaggtcac atttttcccg acactagcca tagcacgata
     2641 cttctgtctt tgaatctcaa catcaacgac ataaacgcca tctgctggca tataagtacg
     2701 gtctaaaagc actaaattcg ctgtcggata accaattgta cgaccacgag cattaccatg
     2761 aaccaccata cctcttgatg gaagcggtgc ccccaaaagt tttcctgctt ctttcacatt
     2821 tccatctaaa atagcttgac ggatacgagt tgaactaatc tttcctttct catcttctac
     2881 aggtggaaca atgataactt ctccatcaaa gtaatccttt aaatcttctg ctgttttttt
     2941 gtcagaacca aatgtataat caaaacctgc aacaataatt ttggcattca tagccttgat
     3001 ataagttgca aagaattctt gtgcagtgag actagcgaat tgactactaa aatcaaggag
     3061 atataattct tctacacctt cgcgctttaa ttttctttca cgttcagcag ggttcaaaat
     3121 atgcaaaaac aaatctggat gataaggctc taaagcgatc tttggagatt cattaaaggt
     3181 cataacgacg ataggcaata aatcctttct cgcagccttg ttggcaacac gaaataattc
     3241 ttgatgcccc ttatgtatgc catcaaaata gccgagaaca acgactgaat cagatggtgt
     3301 gccaatatct ttttggtttt ttataggaat agtaataatc ataaaataat tatatcatag
     3361 cgatagctat ttctggaaca gaaaatctga aatgttgttt ttttcacatg aagtgtacct
     3421 gttttcaaaa agcactttat tctatcgttg cttaactatg aactttgcaa tattcttctc
     3481 aaaaacttgt aggacatctt caaaattttg caaggagtga ttagacttgt tcggtaacca
     3541 taaagtgtca tactatgtta tgtatgaaaa agcaatgcaa ctaactcctg agaactttaa
     3601 attactaatt ggtgccgaaa aggtagaatt tagaatcgag gtacacctat ggctgtaaaa
     3661 tttacaaaat gagacaactt gggcaagatg tttgaagaat ttcctaaact ccctgatttg
     3721 aagcaagtca ctttccctga tgacaaagaa aaaagccaaa acagcaaaga aaaactagat
     3781 gactgctttc caacaactcc catctagtgt gcttcagact gggctatttt tctctccatc
     3841 tgttagcttg gattctcaga ccgtttcagc taaagaatat cttttccctt atcagaagga
     3901 acggctcaaa ccattcagac aagtgaaggg atgacaagcc aatatttgaa accagatagc
     3961 agttcttata gtcaattgaa ataaaatctg aagaaatcga gtaggaaact catatcaatt
     4021 tttaacagtg ttctattcca gattcatact caatgaaaat taaagtgcaa actaggaagt
     4081 tagccgcagg ctgtacttga gtacggcaag gtgaaattga cgtggtttga atttgatttt
     4141 cgaagagtat caatccacta tacatattaa aaagtatggg tatcaaaata aaaaaacagg
     4201 ctctccgaaa actcggaaag ccttatttta agctgtttct agcttcctca cctttacatt
     4261 tcaaggttcg ggataaaagg ttcaatgaac ctttttattt tgcgattggg taaacagaaa
     4321 cttgtttttt atcgcgtcct ttacgttcaa agcgtactac gccttcaact ttagcgaaca
     4381 aagtatcgtc tccaccacga ccaacgttta cacctggata gatgtgtgta ccacgttgac
     4441 ggtaaaggat tgatccacct gttacagttt gtccgtcagc tgctttagct ccaagacgtt
     4501 ttgcttgtga atcacgtccg tttgatgtag aacctccacc ttttttgtgg gcgaaaagtt
     4561 gcaagttgtt aagagtcatt tttaacataa tgttttcctc cgtgttagtt ttctgtgata
     4621 actctggttt ggacgaactc agaagagttc tccgataagt ttgccatacc taagaaaaat
     4681 gattcaaaga ataactgggt catttctctc tggtgtgaag gaagatcttt tggtatttca
     4741 accatcagat agccaccttc atcttcgttt aattctagga ttggttcata gcctgcaaat
     4801 ttctcaatag aattgataaa gttaatggca agcgtagaaa ccgatgcaca cacgacatct
     4861 aagccgtatt cgccactctc ggcgtgtcca gtaatttccg cactcctcag ctcgccatct
     4921 tcggctctct caaagactgc ctgtatcatg tgttctcctt aaaattaagc gttgattgcg
     4981 ttgatgacaa cttttgtata tggttgacgg tgaccttgtt tacggtggct acctttttta
     5041 ggtttgtact tgtaagtaac cactttcttt tgttttcctt gtttttcaac agttccaact
     5101 acagtagctc cagcaacaag tggagttccg acaacagtgt tttcaccacc aacaagaaca
     5161 acttcgttaa aagtaacttc ttgaccagct tcaacgttca atttttcaac gtaaactgct
     5221 tgaccaactt caactttaac ttgttttccg ccagttttga taattgcgta tgtgctcatt
     5281 atgcacctcc tatgattttt atgggtttcc ccgatttttt gtgaagactc gcctagcatc
     5341 gtgggacgaa ccacttaaac ttgaataatc aagcaacgat gttcgtgcgg ttgcacagga
     5401 tcgtgcatag tcaactcttc aagtatagca tatctcctat tttcttacaa gtaataacac
     5461 ctaaaatgaa gctttttctt ttactttttt ctgccaagag gcaaaaagca tgctgaggta
     5521 aaaaacgctc atcataatag gaacaccaag aatggtcttt tcatgataga aaatcgtcaa
     5581 ataggctgaa aagacaacgc caaggacaaa actactaagc aggctaacaa atatgaatcc
     5641 ttcacgcaaa aaaggagtgt gcttggttcg gaaataatct ccaaaagcca gcatggtccg
     5701 tttgatattc cctgtcataa aagcgttatt ataggcaata cccgacactt ctccaaaagc
     5761 agttgtcacc agtcccatac agaaggccaa gggcggcact agatagatat tatccacagt
     5821 ttgcggcaca aaagcaataa tgattgataa gattgccaag ggaatcaagg acagaatagg
     5881 ttttttcaca attctcaatt tttccttata aatcgttaat aaaaagactc ccatcataaa
     5941 cgctagcaag gtgagaacct tgtccctaac atccgaaaca ttatttttaa ttaattctac
     6001 tgaaagaaag acaacatttc cagtttgtcc agctacaagg gtattcccgc gaacaataaa
     6061 agtgtaagca tccacatatc cagcacaaaa cgtcaaaaaa agtgctaacc ttttagactg
     6121 acgtgatatt tttcttatag gtaataacct cattttacct cccattgtat tttctcttag
     6181 aaatattgta ccattttctt tctaaaaaat cgtaggctac catttagatt ttactattag
     6241 cataaaaata ataatagaca actatttatc caaaaataga tagatgtaac atgtttgcaa
     6301 acaaagcata cgaaccttta gtaaaatcat ttccatgaaa ctagaataga gccctcttag
     6361 caaaaatcat tattttaatt tatttctaat cactccttga cataaataac tctcaccaat
     6421 aaaagactat gtcttaaaaa aatggtataa taaaatcaat acttgggctt gatggctatg
     6481 ctactaataa caattaggag agaaaatcag gcacttgtta acaacaagga ttatcccctt
     6541 gagatgaaag gaactttaga aatcttatga tgaacatgca aaacatgatg cgtcaagcac
     6601 aaaaacttca aaaacaaatg gaacaaagcc aagctgaact tgctgctatg caatttgttg
     6661 gcaaatctgc tcaagatctt gtccaagcga ccttaactgg cgataagaaa gttgtcagca
     6721 ttgatttcaa tccagctgtc gttgacccag aggaccttga gactctttct gatatgaccg
     6781 ttcaagccat caactctgct cttgaacaaa tcgatgaaac taccaagaaa aaactgggtg
     6841 ctttcgctgg gaaattacct ttctaaaaac aaggagctag aacaatgctt gtcgataaca
     6901 aaggctaaga aaggtgcaaa aatgactcta taatatttgt agtgggtaaa tccactatag
     6961 atattatgga gcctatttta
//
LOCUS       DBERDK010000087         6883 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.087, whole genome shotgun sequence.
ACCESSION   DBERDK010000087 DBERDK010000000
VERSION     DBERDK010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6883
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.087"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            87..3437
                     /locus_tag="ACL73L_001868"
     CDS             87..3437
                     /locus_tag="ACL73L_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HGS0772790.1"
                     /translation="MGNFSFLLKNDEYESFSKPCIEAENMIATSTVATAFMARRALEQ
                     AVHWIYSHDSYLEAPYRATLSSLVWDDDFRDIVDSELHKQIVLLIRWGNHAAHGGEIK
                     EREAILALHHLYQFVNFIDYCYSNEFVERYFDEKCLPLSANIKYRETPQSMIKLQDSL
                     PELPDFHEQMAAQSVEVQETYTEKRETAAQRQDVPFHIDQLSEAETRKLFIDIDLRLA
                     GWIFEENCRVEIAVDGLKHGSGIGYCDYVLYGKNGKILAIVEAKKASVNPEVGEVQVK
                     EYAEALEKHIGYQPICFITNGLKHYILDGPNRRQIAGFYSQEELQLVMDRRHLQKPLE
                     DISSKIRDDISGRHYQKHAIASVCEAFSNHRRQALLVMATGAGKTRTAVSLVDILSRH
                     NWVKNVLFLADRTSLVKQAYDSFRKLLPDLSVCNFLEDQEGAQSSRMVFSTYPTMIGA
                     ISGQEEVNQRPFTVGHFDLIIIDESHRSIYQKYKSIFDYFDARIVGLTATPRQDLDKN
                     TYGFFNLENGVPTYAYDLEEAVKDGYLVAYHSIETKLKLPTDGLHYDDLSEEEKEHFD
                     SKFEDNSCEKDIDGSVFNSFIFNKSTVEIVLNELMTRGIQTASGDEIGKTIIFAKNHD
                     HAEYIRGIFNNRYPEKGSDYAQVIDYSIKHYQTLIDDFKIKEKYPQIAISVDMLDTGI
                     DVPEVVNLVFFKKVRSKTKFWQMIGRGTRLCKDLFGPEQDKENFLVFDYGDNFDYFRA
                     DPRDGEGRHIVSLTQRLFNIKVDLIRELQGLQYQEDQFARAYRQQLVSELQGRIESLN
                     ELDFRVRMVLDTVYSYRKLESWQNLTAVTSETIQKNLSPLLFDEDKEDEMARRFDLWL
                     LHIQLGQLTAKSSTVHISQVMKTARALSAIGNIPQVFEQAEIIRKVQEPEFWKEVNLS
                     DLEKIRLAIRDLLQFLDKTDRKPYYVNFEDRILSTVHETTAFLQVNDLRSYNEKVEHY
                     LKTHLDEESISKLYHNKKLTSDDMLALEKLLWEKLGSKADYQSHYENKAIPRLVREII
                     GLDRESANRIFSKFLSDENLNARQISFVKLIVDYIVENGFLETKVLTQEPFKSYGSVQ
                     LLFQHQLPVLRNIVQIIELINNRAGEAA"
     gene            complement(3942..4412)
                     /locus_tag="ACL73L_001869"
     CDS             complement(3942..4412)
                     /locus_tag="ACL73L_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HGS0772791.1"
                     /translation="MNKSEHRHQLIRALITKNKIHTQAELQALLAKNDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFAPPFFFEE"
     gene            complement(4429..6702)
                     /locus_tag="ACL73L_001870"
     CDS             complement(4429..6702)
                     /locus_tag="ACL73L_001870"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HGS0772792.1"
                     /translation="MRFNQYSYINFPKENVLSELKKCGFDLQNTANHKDSLETFLRRF
                     FFTYQDTNYPLSILAADKKTDLLTFFQSEDELTADIFYTVAFQLLGFSYLVDFEDSDV
                     FRKETGFPIIYGDLIENLYQLLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLAT
                     FSNQDVIREVVYVESRVDTDQKGLSDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKA
                     SDKALYKMEVELEVKLPHKIELEEPQLNLVQPQGQAELVAEAEEKLTHINSSYTLNDY
                     FLPRGFANLYVSGVGTKDSTGFMTNGDYQQIEAYKNVIDWLNGRCRAFTDHTRQRQVK
                     ADWSNGKVATTGLSYLGTMSNGLATTGVDGLEVIIAEAGISSWYNYYRENGLVTSPGG
                     YPGEDFDSLAELTYSRNLLAGDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWHDRNYL
                     LNAHKVKAEVVFTHGSQDWNVKPLHVYQMFHALPTHIHKHLFFHNGAHVYMNNWQSID
                     FRESINALLTKKLLGQETDFQLPTVIWQDNTAPQTWLSLDNFGGQENCKTFSLGQEEQ
                     AIQNQYPDKDFERYSKTYQTFNTELYQGKANQITINLPVTKDLHLNGRAQLNLRIKSS
                     TNKGLLSAQLLEFGQKKYLQPYPAILSARTIDNGRYHMLENLCELPFRPEAQRVVTKG
                     YLNLQNRNDLLLVEDITADEWMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITIRDNT
                     DYHLTVDLAQSMLTLPC"
BASE COUNT         2117 a         1151 c         1488 g         2127 t
ORIGIN      
        1 gcttgtgaga tagctataaa agtgtggaga aatagcttga aatttagtat aatatagcta
       61 aactatttgt ttaaagtgag aaaaaaatgg gaaattttag ctttctttta aaaaatgacg
      121 aatatgaatc tttttcaaaa ccttgcattg aagctgagaa tatgattgct acatcaactg
      181 tggctactgc ctttatggcg cgtcgtgctt tagagcaggc tgtccattgg atatatagtc
      241 acgattcata tttagaagct ccctatcgtg ctactctatc ttctttagta tgggatgatg
      301 attttaggga tatcgtagat tctgaactcc acaagcagat agttctgttg attcggtggg
      361 gaaaccatgc tgctcatggt ggtgaaatta aggaacgaga agcgatttta gctttgcatc
      421 atttgtatca gtttgttaat tttatcgatt attgttacag caatgagttt gtggagcgtt
      481 attttgatga gaagtgctta ccactttcag caaacatcaa ataccgagaa actccacaat
      541 ctatgataaa gttacaagac agtttaccag aactgcctga ttttcatgaa cagatggctg
      601 ctcagtccgt agaagttcaa gagacttata ctgaaaaacg tgagactgca gcgcaacggc
      661 aagatgtgcc tttccatatt gatcaattat ctgaggcaga gacaagaaag ctctttattg
      721 atatcgatct ccgtttagca ggatggatat ttgaagaaaa ctgtcgtgtt gagatagccg
      781 ttgatggtct caagcacggt tcaggaattg gttactgtga ctatgtactt tatggtaaaa
      841 atgggaaaat tttagcgatt gtggaggcta aaaaagcctc tgtcaatcca gaagtagggg
      901 aagtacaggt caaagaatat gctgaagctt tggagaaaca tatcggctat cagccaattt
      961 gctttattac aaatgggttg aagcactata tacttgatgg tccgaaccgc cgccagattg
     1021 caggctttta ctctcaagaa gaattgcaat tagtgatgga tagacgtcat cttcaaaaac
     1081 cgcttgagga tatttctagt aaaattaggg acgatatttc cgggcgtcac taccaaaaac
     1141 atgccattgc aagcgtttgt gaagctttct ctaatcatcg tagacaggca cttttggtta
     1201 tggcaactgg ggcggggaaa actcgtacag cagtttctct agttgatatc ttatcacgtc
     1261 ataactgggt aaaaaacgtt ctcttcttag ccgatagaac ttccttggtt aagcaagcct
     1321 atgattcgtt tagaaaatta ctcccagatc tttccgtttg taacttctta gaagatcaag
     1381 aaggagctca atcaagtcgt atggtctttt caacttatcc gaccatgatt ggagcgatta
     1441 gtggtcaaga agaagtaaat caacgccctt ttactgttgg gcattttgac cttatcataa
     1501 ttgacgaatc tcaccgttct atttatcaga aatacaagtc catttttgat tattttgatg
     1561 caagaattgt aggcttaaca gctactccgc gtcaagattt agataaaaac acctatggat
     1621 tctttaattt ggagaatggg gttccaacat atgcatatga tttggaagag gctgttaaag
     1681 acggatattt agtagcctat cattctatcg aaaccaaact gaaactacct acggatggtc
     1741 tacattatga tgatttgtcc gaagaagaaa aggaacattt tgatagcaaa tttgaagaca
     1801 atagctgtga aaaagatatt gatgggagtg tatttaattc ctttattttc aataaaagta
     1861 cagtagaaat tgttttaaat gaactcatga caagaggaat tcagacagcc tcgggtgatg
     1921 aaattggtaa aactattatt tttgctaaaa atcatgacca tgcggaatat atcagaggta
     1981 tttttaacaa ccgctatcct gaaaaaggga gcgactatgc tcaggtgatt gattatagta
     2041 ttaagcatta tcagaccttg attgatgatt ttaaaattaa ggagaagtat cctcaaattg
     2101 cgatttctgt cgatatgtta gatacaggta ttgatgtacc agaggttgtt aatttagtct
     2161 tcttcaagaa agtacgctct aaaactaagt tttggcagat gattggtcga ggaacccgtc
     2221 tatgtaaaga tttatttgga cctgagcagg ataaggaaaa cttcttggta tttgattatg
     2281 gggacaattt tgattatttt cgtgcagatc caagagatgg agagggtcgt cacattgttt
     2341 cgctgactca gcgtttattt aatatcaaag tggacttgat tcgagaactt cagggactcc
     2401 aataccaaga agatcagttt gcgagagcat accgtcagca gcttgtctcg gaacttcaag
     2461 gtcgtataga gagcttaaat gagttggact tcagggttcg tatggtttta gatacagttt
     2521 atagctatag gaaattggaa agttggcaga atctaactgc tgttacaagt gaaaccattc
     2581 aaaaaaatct ctctccgctt ttatttgatg aagataaaga agatgagatg gcgaggagat
     2641 ttgatttgtg gttgcttcat attcagttgg ggcaactgac agctaaatct tccactgttc
     2701 atatttccca agtgatgaag acggctagag ctctttctgc tattggcaat atcccgcagg
     2761 tttttgagca ggctgaaatt atcaggaaag tacaggagcc tgaattttgg aaagaagtta
     2821 acttgtctga tttggaaaaa attcgtcttg ctattcgaga tttattacag tttttggata
     2881 aaacagaccg taaaccctac tatgttaact ttgaagatcg tatactctcc actgttcacg
     2941 agaccacagc atttttgcag gtcaacgatc ttcggtctta caatgaaaaa gttgagcatt
     3001 atttgaaaac tcatctggat gaggagtcca tttctaagct ataccataat aaaaagttga
     3061 catctgatga tatgcttgca cttgaaaaat tgctctggga aaaattaggt agtaaagcag
     3121 actaccaaag tcattatgaa aataaggcaa ttccgagatt ggttcgtgag attattggct
     3181 tagatagaga gtctgccaat cgtatttttt ctaaattttt gtcggatgag aatcttaatg
     3241 ccaggcagat ttcatttgta aaattgattg tagactacat tgtagaaaat ggttttttag
     3301 agacgaaagt gttaacgcaa gagccgttta aatcttatgg ttctgttcaa ctactcttcc
     3361 aacaccaact accagtactt cgtaatattg ttcaaatcat tgaacttatc aataatcgag
     3421 ctggagaagc ggcttaaatt ctaaagtgat tgccatgctg agactcattt aaaattaaaa
     3481 agagtagaaa tttatgctat atatgagaag ttttattagg aagaatgtca tcgttttcct
     3541 agaatacagt atcagttgtt aagtggttga taaatttcaa agtagatact tgtaccacga
     3601 tgtttgttga tcgagttatt aacaaaagag ctactttgat tttaaagaaa tagaaaacaa
     3661 aaagctgagc aagaattcaa ttgcaggaga aaatgaaata atactcaatg aaaatcaaag
     3721 agcaaactag gaaactagct gcaggctgct caaaacactg ttttgaggtt gcagatggaa
     3781 gctgacgcgg attgaagaga ttttcgaaga gtataaatct tcctaggata aagcaaaacg
     3841 catagtatca agggttttca acacttgata ctatgcgttt tctgatgtta aagactttct
     3901 accagttttt tttaagcata attgttagtt gtagtcattt attattcttc aaagaaaaat
     3961 ggtggggcga attttttcag ttcttcaaag cacttttgag cagtatctgc atcttcacag
     4021 atgataagac agacatcatt accacaaagg gtagcgatag cgtcagggaa gctcaaagta
     4081 tcaatgatag aaccaaagga ttgagccagt ccaggaaggg tttttagtag gacttggtgt
     4141 tgaactgggc gcatccagac aagggcgtct tccatgtaga gttcgagacg tttttcccat
     4201 tttgagatgg aaccattgtt aagaacataa taagcgctat cttcttcgcg gacttttgat
     4261 aggttcatat ttttgatgtc gcgtgagagg gttgcctggg ttacttgaat gtcgttctta
     4321 gcaagaaggg cttgcaactc agcctgtgta tgaatcttgt tttttgtgat aagagcgcgt
     4381 ataagttggt ggcggtgttc tgatttattc ataataatgt aactcctttt agcaaggtaa
     4441 ggtaagcatg gactgagcga ggtcgacagt caagtggtag tctgtattgt cacggatggt
     4501 gatttcaaag tcagtagtat agaggactaa acggagagtg tctccttctt ttagcttgta
     4561 aatagttggc tgcagttcaa attgaacatc catccattca tctgcagtaa tatcctctac
     4621 taacagtaaa tcatttctat tttgtaaatt aaggtaacct tttgtcacga ctcgttgtgc
     4681 ctctggtcta aatggcaatt cacagagatt ttccaacatg tgatagcgac cgttgtcaat
     4741 ggttctagca cttaaaatag ctggataagg ttgtaggtat ttcttttgcc caaattctag
     4801 cagttgggca gataagagcc ccttgtttgt actggatttg atacgaagat tgagctgagc
     4861 gcgaccgttt aggtggagat ctttagtcac aggaaggtta atagtaatct gattagcttt
     4921 cccttgatag agctctgtat tgaaggtttg gtatgtctta ctatagcgct caaaatcctt
     4981 atctgggtac tggttttgaa tagcttgctc ttcttgacca agtgagaagg ttttacagtt
     5041 ttcttgccca ccgaagttat caagtgataa ccaagtctgt ggagctgtat tgtcctgcca
     5101 gataacagta ggaagttgaa agtctgtttc ctgtcctagt aatttcttgg ttaataaggc
     5161 atttatggac tcacggaagt caattgattg ccaattgttc atgtaaacat gggcaccatt
     5221 atggaaaaag agatgcttgt gtatatgagt aggaagagca tggaacatct ggtaaacatg
     5281 aagtggtttg acattccaat cctgagaacc atgagtaaag acaacctctg cctttacttt
     5341 atgggcattg agcagataat tgcggtcatg ccaaaactga ttgtagtccc cagtttttcg
     5401 gtctagctga gctttcactt tttctaagtc agcttggtga gcttcattgc cacggatata
     5461 gtcgccagct aagagattac gagaataggt taactcagca agggagtcaa agtcctcacc
     5521 tggataacca cctgggctag tcaccagacc gttttcacgg tagtagttgt accatgatga
     5581 aattcctgcc tcggcaatga taacttctaa accatcgact cctgtagtcg caagaccatt
     5641 ggacatggta cctagatagg aaagtcccgt tgtagcaact tttccgtttg accaatcagc
     5701 cttgacttga cgctggcgcg tgtgatcagt aaaggcacgg caacggccgt taagccaatc
     5761 gatgacattt ttataagcct cgatttgctg gtagtctcca ttagtcatga aacctgtcga
     5821 gtctttggta ccaacacctg agacatagag attggcaaag cctctcggaa ggaagtagtc
     5881 gtttagtgta tagctagagt tgatgtgagt tagcttttcc tcagcctctg ctacaagctc
     5941 agcttgacct tggggttgga cgagatttag ttgaggttcc tctagctcaa tcttgtgagg
     6001 aagcttaacc tcaagctcaa cctccatctt gtagagagcc ttgtcactag ccttgtcatt
     6061 ggttccctga tgataagggc tggctgtcat gatggcaggg atttttccat caaaacgagg
     6121 gcgaataatg ctaaccttta ctaggtctga tagccctttt tggtcagtat cgacacgaga
     6181 ctcaacgtaa acgacttcac gaatgacatc ctggttagaa aaagtagcca aactcttgcc
     6241 gttaaagtag tggtagtcat tatcctccgg aataagacca tcactaacaa gttggtcgat
     6301 aagagtattt ccttttttgg tgcgagtatt gagtaactga tagagatttt caatcaagtc
     6361 accatatata atgggaaatc cagtttcttt acgaaaaacg tcactatctt cgaagtcaac
     6421 caaataagaa aagcctaaaa gttgaaaagc aacagtataa aaaatatctg ctgtcagttc
     6481 atcttctgat tgaaaaaatg tcagcaggtc tgttttttta tcagctgcta ggatagaaag
     6541 tgggtagttg gtgtcttgat aagtgaaaaa gaaacgacgt aaaaaggttt caagtgagtc
     6601 tttgtgattg gctgtatttt gtaaatcaaa gccacatttt tttagctcag ataagacatt
     6661 ttcttttgga aaattgatat aactatattg attaaaacgc atagaacctc catatagaat
     6721 gacagttaag gttattatat caaaaaaaaa gcagaaaggg aattgttaac ttcaaaagga
     6781 aataatccaa taaaaatgaa taaagtacta aattcaatat agagaacaga gtaacaataa
     6841 gaataataga tagggtataa aagttctagg agatttatat tat
//
LOCUS       DBERDK010000088         6871 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.088, whole genome shotgun sequence.
ACCESSION   DBERDK010000088 DBERDK010000000
VERSION     DBERDK010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6871
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.088"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(39..380)
                     /locus_tag="ACL73L_001871"
                     /pseudo
     CDS             complement(39..380)
                     /locus_tag="ACL73L_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844241.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            578..2344
                     /gene="patB"
                     /locus_tag="ACL73L_001872"
     CDS             578..2344
                     /gene="patB"
                     /locus_tag="ACL73L_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
                     /protein_id="HGS0772793.1"
                     /translation="MKTVQFFWHYFKVYKFSFVVVILMIVLATFAQALFPVFSGQAVT
                     QLANLVQAYQNGNPELVWQSLSGIMVNLGLLVLVLFISSVIYMCLMTRVIAESTNEMR
                     KGLFGKLAQLTVSFFDRRQDGDILSHFTSDLDNILQAFNESLIQVMSNIVLYIGLILV
                     MFSRNVTLALITIASTPLAFLMLIFIVKMARKYTNLQQKEVGKLNAYMDESISGQKAV
                     IVQGIQEDMMAGFLEQNERVRKATFKGRMFSGILFPVMNGMSLINTAIVIFAGSAVLL
                     NDKSIETSTALGLIVMFAQFSQQYYQPIIQVAASWGSLQLAFTGAERIQEMFDAEEEI
                     RPEKAPTFTKLQESVEISHIDFSYLPDKPILKDVSISAPKGQMTAVVGPTGSGKTTIM
                     NLINRFYDVDAGGIYFDGKDIRGYDLDSLRSKVGIVLQDSVLFSGTIRDNIRFGVPDA
                     SQEMVEVAAKATHIHDYIESLPDKYDTLIDDDQSIFSTGQKQLISIARTLMIDPEVLI
                     LDEATSNVDTVTESKIQHAMEVVVAGRTSFVIAHRLKTILNADQIIVLKDGEVIERGN
                     HHELLKLGGFYSELYHNQFVFE"
     gene            2483..3172
                     /locus_tag="ACL73L_001873"
     CDS             2483..3172
                     /locus_tag="ACL73L_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HGS0772794.1"
                     /translation="MARTVVGVAANLCPVDAEGKIIHSSVSCRFAEIIRQVGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTSHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLNEL"
     gene            3176..3355
                     /locus_tag="ACL73L_001874"
     CDS             3176..3355
                     /locus_tag="ACL73L_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772795.1"
                     /translation="MEHLVVLSFIFSKFLECGILRKRLNYDKNWRNSTKKLEKNEGKR
                     YDRKEEILEEEHVTY"
     gene            3342..4691
                     /locus_tag="ACL73L_001875"
     CDS             3342..4691
                     /locus_tag="ACL73L_001875"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HGS0772796.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEQIKSDSDVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADLVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANGWGTFVVPDDIGGRFSVLTAVGLL
                     PIAASGADIKALMEGANAARKDYTSDKISENEAYQYAAVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVLIPTLEEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
     gene            4815..6275
                     /gene="gltX"
                     /locus_tag="ACL73L_001876"
     CDS             4815..6275
                     /gene="gltX"
                     /locus_tag="ACL73L_001876"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HGS0772797.1"
                     /translation="MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIE
                     DTDRKRHVEDGEHSQLENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWIIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     ADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEA
                     EREVMTGETVPTVLEAFKAKLEAMTDDKFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIFLLGREKSIQHIENMLKEISK"
     gene            6289..6723
                     /locus_tag="ACL73L_001877"
     CDS             6289..6723
                     /locus_tag="ACL73L_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HGS0772798.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYANHVVAAVEAEDGQIFTGF
                     CMEGTCGVFHLCAERAALFNMYQFSGQTKVKKVLAFRDKPPYGGSSAMPCGACREFLL
                     ELNAENKDAEFMMDYNIRKTVKVAELIPYWWGEERASKFNER"
BASE COUNT         2163 a         1309 c         1483 g         1916 t
ORIGIN      
        1 tttgcaaggt ggatgatggc tacattgtaa tgttttcctt attctaactt agtcttaaga
       61 taggccttaa aagcaggtga aaagcgaggg catgctttgg cagcttgtat gagtgcctac
      121 cgcagatgag gggaaccccg tttgatcatt cttccagcta aatcaatctg atctgactga
      181 taaatagaag aatccagtcc agcgaaagct tgtaattgag caagattatc aaaggcatga
      241 atatttcgaa tctcggctaa aatgaccgcc cctaaacgat tcccaatccc agtaaccgtc
      301 gtgatgatcg agttgaactc agccatcaag tcattgacac atgtttccgc cttgtcaatg
      361 agcctcttgt aatgtttgat gttttcatta cacgagataa aacgtttatg cgttatcaaa
      421 ctcatgacca attgaaacaa aaagctgtgg ttagatcctt tcggaaattg tcaagcgatt
      481 ggaggaaatg aactaatcca cagcggctta ttccaagtat accacttggg ctttggcagt
      541 agctaactgc gctaaatata atataaggag gagtaaaatg aagacagttc aatttttttg
      601 gcattatttt aaggtctaca agttctcatt tgtagttgtc atcctgatga ttgttctggc
      661 gacttttgcc caagccctct ttccagtctt ttctggacaa gcggtgacgc agctagccaa
      721 tttagttcaa gcttatcaaa atggcaatcc agaacttgta tggcaaagcc tatcaggaat
      781 catggtcaat cttggcctgc tggttttggt tctatttatc tctagtgtaa tatacatgtg
      841 tctcatgacg cgcgtgattg cagaatcgac caacgagatg cgcaaaggcc tctttggtaa
      901 gcttgctcag ttgacggttt ctttctttga ccgtcgacaa gatggcgata tcctgtctca
      961 ttttaccagt gatttggata atatcctcca agcctttaac gaaagcttga ttcaggtcat
     1021 gagcaatatt gttttataca ttggtctgat tcttgtcatg ttttcgagaa atgtgacgct
     1081 ggctctcatc accattgcca gcaccccatt ggctttcctt atgctgattt tcatcgtgaa
     1141 aatggcacgc aaatacacca acctccagca gaaagaggta gggaagctca acgcctatat
     1201 ggatgagagc atctcaggcc aaaaagccgt gattgtgcaa ggaattcaag aggatatgat
     1261 ggcaggattt cttgaacaaa atgagcgcgt gcgcaaggca acctttaaag gaagaatgtt
     1321 ctcaggaatt cttttccctg tcatgaatgg gatgagcctg attaatacag ccatcgtcat
     1381 ctttgctggt tcggctgtac ttttgaatga taagtctatt gaaacaagta cagccctagg
     1441 tttgattgtt atgtttgcac aattttcaca gcagtactac cagcctatta tccaagttgc
     1501 agcgagttgg ggaagccttc agttggcctt tactggagct gaacgaattc aggaaatgtt
     1561 tgatgcagag gaggaaatcc gacctgaaaa ggctccaacc ttcactaagt tgcaagaaag
     1621 tgttgaaatc agtcatatcg atttttcata cttgcctgat aaacctattt tgaaagatgt
     1681 cagcatttct gcccctaaag gccagatgac agcagttgtt gggccgacag gttcaggaaa
     1741 aacgactatt atgaacctca tcaatcgctt ttatgatgtt gatgctggtg gtatttattt
     1801 tgatggtaaa gacattcgtg gctatgactt agatagtctt agaagcaagg tgggaattgt
     1861 attgcaagat tcggtcttgt ttagcggaac gattagagac aatatccgat ttggtgtgcc
     1921 agatgctagt caggaaatgg ttgaggtagc agcaaaagca acccacattc acgactatat
     1981 cgaaagtttg cctgataagt acgatactct tattgatgat gaccagagca tcttttcaac
     2041 agggcagaag caattgattt caatcgctcg aaccctgatg atagatccag aagttctcat
     2101 tctcgatgaa gcaacttcaa acgtagatac ggtgacagaa agcaagattc agcatgccat
     2161 ggaggtggtt gtagcaggta gaactagttt cgtcattgcc caccgcttga aaaccattct
     2221 caatgcagat cagattattg tccttaaaga tggagaagtc attgaacgtg gtaaccacca
     2281 tgaacttttg aagctaggtg gcttttattc agaactctat cacaatcaat ttgttttcga
     2341 ataagaaaga agttgtccta tgtgggcagc tttttcttgt ccataaaaaa tgtttatcac
     2401 agccttaaaa aaaacatatt agacgaaagt cattttgagt gatatgatag gactatcgtt
     2461 agcattcgaa aggagaggca tcatggctag aacggttgta ggagttgctg caaatctatg
     2521 tcccgtagac gcagaaggca aaatcattca ttcatctgta tcttgtagat tcgcagagat
     2581 cattcgtcaa gtcggtggtc tccctttagt cattcctgtt ggtgatgagt cagttgtacg
     2641 tgattatgtg gaaatgattg acaaactcat tttgacagga ggccaaaatg ttcatcctca
     2701 gttttatgga gagaaaaaga ccgtcgagag cgatgattac aatctggtcc gtgacgaatt
     2761 tgaattggca ctcttgaagg aagcgcttcg tcagaataaa ccaattatgg caatctgtcg
     2821 cggtgtccaa cttgtcaatg ttgcctttgg tggaaccctc aatcaagaaa tcgaaggtca
     2881 ctggcaagga ctacctttcg ggacatctca ctctattgag acagtagaag gaagcgtggt
     2941 ggctaagcta tttgggaaag aaagtcaggt caactcagtc catcgtcaaa gcattaaaga
     3001 tttggcacct aatttccgtg taactgctat tgattcaaga gaccaaacta ttgaagcgat
     3061 tgagtctatc gacgagcacc gcattatcgg tttacagtgg catccagagt ttctggttaa
     3121 tgaagaagat ggcaatttag aattatttga gtatttattg aatgaactgt aacgattgga
     3181 acatctggtc gttctttctt ttattttttc aaaattttta gaatgtggca ttttacgcaa
     3241 acgtttgaat tatgataaaa attggagaaa ttcgacaaaa aaacttgaaa aaaacgaagg
     3301 taagcgttat gatagaaaag aagaaatatt ggaggaagaa catgtcacat attaaatttg
     3361 attattcaaa agttttagac aaatttgttg caccacatga agtggaatac atgcaatcac
     3421 aagtaacagc agcagatgaa ttgatccgta aaggaactgg tgctggtagc gacttcttgg
     3481 gatggttgga ccttcctgaa aaatacgatc gcgaagaatt tgaccgcatc ttgaaagctg
     3541 ctgagcaaat caagtcagac agcgatgttt tggttgtgat cggtattggt ggatcttacc
     3601 ttggtgcaaa agcagcaatc gacttcttga atcaccattt tgctaacttg caaacaaaag
     3661 aagaacgcaa agctccacaa atcctttacg caggaaactc aatctcatct acttaccttg
     3721 ctgacttggt agagtatgtt gcagacaaag acttctcagt aaacgtaatt tctaaatcag
     3781 gtacaacaac tgaaccagcg attgctttcc gtgtctttaa agaactcttg gttaagaaat
     3841 acggtcaaga agaagctaac aaacgtatct atgcaacaac tgaccgccaa aagggtgctg
     3901 ttaaggttga agcagacgct aacggttggg gaacatttgt tgttccagat gatatcggtg
     3961 gacgcttctc agtattgaca gccgttggtt tgcttccaat cgcagcatca ggagctgaca
     4021 tcaaagctct tatggaaggt gcgaatgcag ctcgcaaaga ctacacttca gacaaaatct
     4081 ctgaaaacga agcttaccaa tacgcagctg ttcgtaacat cctttatcgt aaaggctatg
     4141 caactgagat cttggtaaac tatgagccat cacttcaata cttctcagaa tggtggaaac
     4201 aattggctgg tgaatcagaa ggaaaagacc aaaaaggtat ctacccaact tcagccaact
     4261 tctcaactga cttgcactca cttggtcaat ttatccaaga aggaactcgt atcatgtttg
     4321 aaacagttgt ccgtgttgac aaacctcgta aaaacgtgct tattcctact ttggaagaag
     4381 accttgacgg acttggttac cttcaaggaa aagacgttga ctttgtaaac aaaaaagcaa
     4441 ctgacggtgt tcttcttgcc cacacagatg gtgatgtacc aaacatgtat gtgactcttc
     4501 cagagcaaga cgctttcact cttggttaca ctatctactt cttcgaattg gcaattgccc
     4561 tttcaggtta cttgaatgct atcaacccat ttgaccaacc aggtgttgaa gcttataaac
     4621 gtaacatgtt tgcccttctt ggaaaaccag gatttgaaga attgagcaaa gaacttaacg
     4681 cacgtctata atagaagaaa agagtggttt gcccactctt tttactctct ttatccatag
     4741 aaattggact cagccaagac ttgtgatata atatagaaag caaaaaggca gacgcctaga
     4801 taataggaga aactatgtca aaagatatcc gcgtacgtta cgcaccaagt ccaacaggac
     4861 tactacacat cggaaatgct cgtacagcat tgtttaatta cttgtatgcg cgccatcatg
     4921 gtggaacatt tctcatccgt atcgaagata ctgaccgtaa acgccatgtc gaggatggtg
     4981 aacattcaca acttgaaaac cttcgctggt taggcatgga ttgggatgaa agtccagaat
     5041 cacatgagaa ttatcgccag tctgagcgtt tggacttgta tcaaaaatac attgaccaac
     5101 tattagctga aggaaaagcc tataaatctt acgttacaga agaagagttg gcagctgaac
     5161 gcgaacgcca agaagtagct ggcgaaacac cacgctacat caatgaatac cttggtatga
     5221 gtgaagaaga aaaagcagct tacatcgcag aacgtgaagc agcagggatc atcccaactg
     5281 ttcgtttggc tgtcaatgag tcaggtatct acaagtggca tgatatggtc aaaggcgata
     5341 tcgaatttga aggtggcaat atcggtggtg actggattat ccaaaagaaa gacggttacc
     5401 caacttacaa ctttgccgtt gttatcgatg accacgatat gcaaatctct catgttatcc
     5461 gtggagatga ccatattgct aatacaccaa aacagcttat ggtctatgaa gctcttggtt
     5521 gggaagctcc agagttcggt cacatgacct tgattatcaa ctctgagact ggtaagaagt
     5581 tatctaaacg tgatactaat acacttcaat ttatcgaaga ttaccgaaag aaaggttacc
     5641 ttccagaagc agtctttaat tttattgctc ttcttggttg gaacccaggt ggtgaagatg
     5701 agattttctc tcgtgaagaa ctcattaaac tttttgatga aaaccgcctc agcaagtcac
     5761 cagcagcctt tgatcagaaa aaactagact ggatgagcaa tgattatatc aagaatgcag
     5821 accttgaaac catctttgaa atggcaaaac cattcttaga ggaagcaggc cgtttgactg
     5881 acaaggctga aaaattagtt gagctctata aaccacaaat gaaatcagta gatgagatta
     5941 tcccattgac agatcttttc ttctcagatt tcccagaatt gacagaagca gagcgcgaag
     6001 tcatgacggg tgaaacagtt ccaacagttc ttgaagcatt caaagcaaaa cttgaagcga
     6061 tgacagatga taaatttgtg acagaaaata tcttcccaca aattaaagca gttcaaaaag
     6121 aaacaggtat taaagggaaa aatcttttca tgcctattcg tatcgcagtt tcaggcgaaa
     6181 tgcatgggcc agaattacca gatacaattt tcttgcttgg acgtgaaaaa tcaattcagc
     6241 atatcgaaaa catgctaaaa gaaatctcta aataaagagg atacaataat ggacatctgg
     6301 gaaaagatgt acgaagaagc acagaaacta tacaatccac atgaagtatc agactttgtt
     6361 tatgccaatc atgttgtagc tgcagtagaa gcagaagatg gacaaatatt tacaggattc
     6421 tgtatggagg gaacctgtgg tgttttccat ctctgcgcag aacgggcggc cctcttcaat
     6481 atgtaccaat tttcaggaca aactaaggtt aagaaagtat tagcctttcg agacaaacca
     6541 ccttatggtg gaagttcagc catgccttgc ggtgcttgta gagaattcct tttagagctt
     6601 aatgctgaaa ataaagatgc agaattcatg atggactata atataagaaa aacagttaaa
     6661 gtcgcagaac taatccctta ttggtgggga gaagaacgtg cttctaagtt taatgagcga
     6721 tagaagagtc agtataattc tggctttttt tactataagt tgaaaaaata aaaaaaaatt
     6781 agaaaaagta gttgacaaag tttgaaaaga ctgtataata gtaagagttg aaaataacaa
     6841 ctcaggtccg ttggtcaagg ggttaagaca c
//
LOCUS       DBERDK010000089         6841 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.089, whole genome shotgun sequence.
ACCESSION   DBERDK010000089 DBERDK010000000
VERSION     DBERDK010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6841
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.089"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(1..711)
                     /locus_tag="ACL73L_001878"
                     /pseudo
     CDS             complement(1..711)
                     /locus_tag="ACL73L_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            920..2689
                     /locus_tag="ACL73L_001879"
     CDS             920..2689
                     /locus_tag="ACL73L_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HGS0772799.1"
                     /translation="MEEKVSLKVRVQKLGTSLSNMVMPNIGAFIAWGVLTALFIADGY
                     LPNEQLATVVGPMLTYLLPILIGYTGGYMIHGQRGAVVGAIATVGAITGSSVPMFIGA
                     MVMGPLGGWTIKKFDEKFQEKIRPGFEMLVNNFSAGLVGFALLLLAFYAIGPVVSTLT
                     GAVGNGVEAIVNARLLPMANIIIEPAKVLFLNNALNHGIFTPLGVEQVAQAGKSILFL
                     LEANPGPGLGILLAYAVFGKGSAKSSSWGAMVIHFFGGIHEIYFPYVMMKPTLFLAAM
                     AGGISGTFTFQLLDAGLKSPASPGSIIAIMATAPKGVWPHLNVLLGVLVAAVVSFLVA
                     ALILHADKSTEDSLEAAQAATQAAKAQSKGQLVSTSVDAVVSTDSVEKIIFACDAGMG
                     SSAMGASILRDKVKKAGLEIPVSNQAISNLLDTPKTLIVTQEELTPRAKDKSPSAIHV
                     SVDNFLGSPRYDEIVASLTGASPIAEIEGDIPTSAPVDSQEIDLNHIDAVVVAYGKAQ
                     GTATMGCETIRAIFRNKNIRIPVSTAKISELGEFNSKNIMIVTTISLQAEVQQAAPNS
                     QFLIVDSLVTTPEYDKMAARMYK"
     gene            2713..4668
                     /locus_tag="ACL73L_001880"
     CDS             2713..4668
                     /locus_tag="ACL73L_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HGS0772800.1"
                     /translation="MLLTKREEQLLKAFLHVGKLSMQDMTEILQVSSRTIYRTLSDLT
                     DSMEQYGIEITKHGKYYILTGELDDLPTELEVLVEYSPQERQELITYRLLTESGFVTN
                     EALQECTKVSNVTIIQDISDIDKRLLDFDLKIERQKGYRISGDSVGKRRFLAILLTNC
                     ISVADFSTGNFGSFDILEADRTRLASQIVNKQLSGFPDMDARMKMFFAILLSLIGQEQ
                     NIENSPNTSKQALEISQKIFQAYSKQTAQFYSIQEIIYFASILDELIIKRQDNPLFTE
                     KFDGEFFYNISNLIDTVSMYTKIDFFKDKVLFNFLFHHIRLSLGVPILFQGENLPESV
                     QILVERNKFLYTVISLLVNDIFPKYLHTEYEYGMIALHFISSLGRSPEIYPVRVLLLT
                     DERRVTRDLLVSKIKSVAPFVELIDIQSLVDYHSIDLSQYDYILSTKPLTNQEIDVIS
                     SFPTVKELLELQERLQYVQAHRAIVARDAIAPEKSYDLQDYLISSSQLLSQFELVQLE
                     NNQSFEHTVEQIIQYQKNVSDRDYLTRKLLSHFQNSPMAIPNTGLVLLHSQSSKVTTN
                     SFTMFELKLPISALSMKREEEEVKRCLLMLMSKEASEEARDLMTAISQSIIENHLYTE
                     IYKTGNQSIIYQMLNTIFNEKIKKLEN"
     gene            4670..5107
                     /locus_tag="ACL73L_001881"
     CDS             4670..5107
                     /locus_tag="ACL73L_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGS0772801.1"
                     /translation="MKLEKHLIKLNKQFSNKEEAICYCGQVLYEGGYVNEDYIEAMIE
                     RDKELSVYMGNFIAIPHGTDAAKKDVLKSGITVVQVPIGVDFGNVSNPQVATVLFGIA
                     GIGNEHLEIIQKISIFCADVDNVLKLADAQSKEEVLRLFDAVE"
     gene            5169..6305
                     /locus_tag="ACL73L_001882"
     CDS             5169..6305
                     /locus_tag="ACL73L_001882"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HGS0772802.1"
                     /translation="MKHSVHFGAGNIGRGFIGEILFKNGFHIDFVDVNNQIIHALNEK
                     GKYEIEIAQKGQSRIEVTNVAGINSKEHPEQVIEAIQKTDIITTAIGPNILPFIAELL
                     AKGIEARRVAGNTQALDVMACENMIGGSQFLYQEVKKYLSPEGLTFADNYIGFPNAAV
                     DRIVPAQSHEDSLFVVVEPFNEWVVETKRLKNPDLRLKDVHYEEDLEPFIERKLFSVN
                     SGHATSAYIGAHYGAKTILEALQNPNIKSRIESVLAEIRSLLIAKWNFDKKELENYHK
                     VIIERFENPFIADEVSRVARTPIRKLGYNERFIRPIRELKELSLSYKNLLKTVGYAFD
                     YRDVNDEESIRLGELLAKQSVKDVVIQVTGLDDQELIEQIVEYI"
BASE COUNT         2059 a         1172 c         1363 g         2247 t
ORIGIN      
        1 cacgatttct tagttctaaa taaatccgac gataagcata atttcccttg tgttcgataa
       61 aaatggattg aatttcagct ttaagctctt ggtccttatc tgttttgtct agctgtttca
      121 agtgatagta gtaggtcgaa cgagctagtt taatggattt tagaagaaga tctaacgaaa
      181 actcagtcat taattcttga acaatttctg tctttcttct ttctcttttt cctccttcaa
      241 tcggagttct cttaactttt ttaggatggc attctccgct ctcaggtact cattttctgc
      301 ttgaagacgt tctaattctg tcctctcttc aggtctcgtt tttggattac gtcccatttt
      361 aggtactctc cctcttgttt tctcaacaat agtatacccg tttttcctgt attgtgctag
      421 ccagttaaga agtatcgtac gacttgggag gccgtattca agagaaactc tatctttagt
      481 ccagccttca tgtcagactt tatgaatcat ttcttgtttt aaatcaggag aatagtaacg
      541 attttttcct tttttgacga actctattcc gtaacgatca atcaatttaa tcatgtacct
      601 aagattagaa ttgtttatcc caaatttatt tgaaagcttc tctaagctta agctatatcc
      661 ttgttttcta agttcataga tctgaacttt atcatcataa gttaatttca taataaaaac
      721 accccaaaag ttagattttt tctgtctaac ttttggggtg cagttcatgt acacctgata
      781 tgatgcgttt tataattttt aagccttttt gcccagcctc gtcaaaagta atgttttgac
      841 acaaaatctg tgacaaaact ttagttttaa aggtttttaa ctttgtatat actagtttta
      901 agaaaaggag gatgatctaa tggaagaaaa agtatcattg aaagtcaggg ttcaaaaact
      961 agggacatcg ctttcaaata tggttatgcc caatattgga gcatttattg cttggggagt
     1021 attgactgcc ctctttatcg ctgatggcta tctgccaaat gaacagttag ctactgttgt
     1081 tggtcctatg ttaacgtatt tattgccaat cctgattggt tacacaggtg gatatatgat
     1141 ccatggccaa cgtggtgccg ttgtaggagc tattgctact gttggtgcaa ttacaggttc
     1201 tagtgttcct atgtttatcg gagctatggt aatgggccca ctgggaggat ggactatcaa
     1261 gaaatttgat gagaagttcc aagaaaaaat tcgtcccgga tttgaaatgt tagttaataa
     1321 cttctcagct ggtctcgttg gttttgcatt attgcttttg gctttctacg caatcggtcc
     1381 agtcgtatcg actcttactg gagctgttgg gaatggtgtt gaggctattg tcaatgctcg
     1441 cctccttcct atggctaata ttatcatcga accggctaaa gtccttttcc tcaataatgc
     1501 cctcaatcat ggcattttta ctcctctggg agtagaacag gtagctcaag ctggtaagtc
     1561 aattctcttc ctattggaag ctaatcctgg accaggcctg ggaattctat tagcttatgc
     1621 tgtattcggt aaaggttctg ctaaatcttc ttcttggggg gcaatggtta ttcatttctt
     1681 cggagggatt catgaaattt actttcctta tgttatgatg aagcctactc tatttttagc
     1741 tgctatggca ggaggtatct ctggaacttt tacttttcaa ctcttagacg ctggtcttaa
     1801 atctccagct tcaccaggtt ctattattgc gattatggct acggcgccaa aaggtgtttg
     1861 gccccatcta aatgttcttt taggtgtttt agtggcagca gttgtttctt tccttgtagc
     1921 agcccttatt cttcatgcag acaagtcaac tgaggattcg ctcgaagctg ctcaggcggc
     1981 tacccaagca gctaaggctc agtctaaagg tcagttagta tcaacttctg ttgatgcagt
     2041 tgtttcgaca gactcagtgg aaaaaatcat tttcgcctgc gatgctggta tgggaagctc
     2101 tgctatggga gctagtattc ttcgagataa ggttaaaaaa gcaggtctag agattccagt
     2161 atctaatcag gcaatctcaa atttgcttga tacaccaaaa acattaattg ttactcagga
     2221 agaactgaca ccaagagcta aagacaagag tccaagtgct attcatgttt ctgttgataa
     2281 tttcttaggg tcccctcgtt atgatgaaat tgtagcttca ttaacaggag cttctccaat
     2341 agcagaaatt gaaggagata taccaacttc agcaccagta gatagtcagg aaattgacct
     2401 taaccatatt gatgctgtag tagttgctta tggtaaagca cagggaactg caactatggg
     2461 ctgtgaaacg attcgggcta tctttagaaa caagaatatt cgtattccag tttctactgc
     2521 caaaatttca gaattaggtg aatttaattc taaaaacata atgattgtaa caactatttc
     2581 tttacaggca gaagtgcagc aagcagcacc gaattctcaa tttcttattg tggatagttt
     2641 agtaacaaca ccagaatatg acaaaatggc tgctagaatg tacaaataga actagaggtt
     2701 tctaaattac gaatgctatt aaccaaacga gaagaacaat tattgaaggc tttcctacat
     2761 gtagggaagc tttcaatgca agatatgact gaaatcttac aggtttcatc tagaacaatt
     2821 tatcgaactt tatcagattt gacagatagc atggagcaat atggaatcga aataacgaag
     2881 catgggaaat actatatttt gactggagag ttggatgatt tgccgacaga acttgaagtg
     2941 ttagttgagt atagtcccca agaaagacaa gagttgatta cctatcgcct tctgactgag
     3001 agtggttttg tcaccaatga agcattgcaa gagtgcacga aagtcagtaa tgtaactatt
     3061 attcaggata tttcagatat tgataagcgt cttttagact ttgatctgaa aattgaacga
     3121 caaaaaggtt atcggatttc tggtgattca gttggtaaga gaagattttt ggctatttta
     3181 ctgacaaact gtatctcagt agcagatttt tcaaccggta attttgggag ctttgatatt
     3241 ttagaagcag atagaactag gctggccagt cagattgtta ataagcaact gtcaggtttt
     3301 ccagatatgg atgctaggat gaagatgttt tttgcgatct tgttatctct tataggtcag
     3361 gagcaaaaca ttgaaaattc acctaatact agtaagcagg ctttggaaat ttctcaaaaa
     3421 atttttcaag cttactctaa gcagactgca caattttata gtattcagga aattatctat
     3481 tttgcgagca tcttggatga attaatcatt aaacgtcagg acaatccgct ctttacggag
     3541 aaatttgatg gtgaattttt ctacaatatt tcaaatctga ttgatacggt ttccatgtat
     3601 accaagattg acttttttaa ggacaaggtt ttattcaatt ttcttttcca tcatattcgg
     3661 ctcagtttag gcgtccctat cctttttcag ggtgaaaatt tgccagaatc tgtccagatt
     3721 ttagttgaaa ggaataaatt tctttataca gtcatcagtc ttttagtgaa tgatattttt
     3781 ccgaaatatc ttcatacaga gtatgagtat ggcatgattg ccctacattt tatctctagc
     3841 ttaggccgta gtccagagat ttatccagtc cgtgttttgc ttttaacgga tgaacgtcgg
     3901 gtcactagag atttattagt cagtaaaatt aagagtgttg ctccttttgt agagttgata
     3961 gatattcagt ctctagtaga ttaccacagt attgatctca gtcagtatga ttatatttta
     4021 tctaccaagc cgctgactaa tcaggaaatc gatgtaattt ctagttttcc aaccgtcaaa
     4081 gaattgcttg aattacagga acgacttcag tatgtacagg cacatcgtgc aattgtcgcg
     4141 cgtgatgcta tcgctccaga gaaaagttat gacttgcaag attatttaat atctagtagt
     4201 cagcttttga gtcaattcga gttggttcaa ttggagaata atcaatcatt tgagcacacg
     4261 gtagaacaaa tcatccaata tcagaagaat gtgagtgaca gagattacct aacaagaaaa
     4321 ttgttatctc acttccagaa tagtcctatg gctattccta atactggtct ggtgctttta
     4381 catagtcagt ctagcaaagt aacaacaaat agttttacta tgtttgaact caaactacct
     4441 atctccgcat tgtcaatgaa acgagaggaa gaagaggtca aaaggtgtct gctaatgcta
     4501 atgtctaaag aagctagcga ggaagcgaga gatttaatga cagctattag tcagtcgatt
     4561 attgaaaatc atctttatac agagatttac aagacgggaa atcaatccat tatttatcag
     4621 atgctaaata ctatttttaa cgaaaaaatt aagaaattgg agaactaata tgaaacttga
     4681 aaaacatttg attaagctta ataaacaatt ttctaacaag gaggaagcta tttgttattg
     4741 tgggcaagtt ctttatgagg gtggatatgt taatgaagac tatattgaag ccatgattga
     4801 gcgagataaa gagctatctg tttacatggg taactttatc gccataccgc atggaacaga
     4861 tgcagcaaaa aaggatgtcc tcaagtctgg tattacagtc gttcaagtcc ctataggggt
     4921 tgattttggg aatgtatcta accctcaagt ggcaacggtt ctttttggta ttgctggtat
     4981 tggtaatgaa cacttagaaa ttattcagaa aatttctatc ttctgtgcag atgtagataa
     5041 tgttcttaaa ctagcagatg ctcagtcaaa agaggaagta ttgcgcttat ttgatgctgt
     5101 tgaataattg aatttcgtca tttgtcatct agtatatatg tccctcaaat aggaaaagga
     5161 gaaattgaat gaaacattct gttcattttg gtgccggtaa tatcggtcgt ggttttatag
     5221 gtgaaattct atttaaaaat ggtttccata ttgattttgt ggatgtcaat aatcagataa
     5281 ttcatgctct aaatgaaaag ggcaagtatg aaattgaaat tgcacagaaa ggacagtctc
     5341 gtatagaagt aactaatgtg gctggcatta atagcaaaga acatcctgag caagtcattg
     5401 aagcgattca aaagacggat attattacta ctgcaatcgg acctaatata ctccctttta
     5461 tcgccgaact tctagccaaa ggaatcgaag ctcgccgagt tgcaggaaat acacaggcat
     5521 tggatgttat ggcctgtgaa aatatgattg gcgggtctca atttctttat caagaagtca
     5581 agaaatattt aagtccggaa ggtttgacat ttgctgataa ctacataggt tttccaaatg
     5641 ctgcagtaga caggattgtt ccagcacaaa gtcacgaaga ttcccttttt gttgtggtcg
     5701 agccctttaa tgaatgggtc gtggaaacca agcgtcttaa aaatccagat ttacgtctaa
     5761 aagatgtgca ttatgaagaa gatttagaac cctttattga gcgaaaactt ttttcagtca
     5821 attctggaca tgcaacttca gcttacattg gtgcgcatta tggtgccaag acaattttgg
     5881 aagctcttca aaatcctaat atcaaatctc ggattgaatc tgtattagct gaaattcgga
     5941 gtctcttgat tgccaaatgg aactttgata aaaaagaatt ggagaattat cacaaagtca
     6001 ttatagaacg gtttgaaaac cctttcatag cggacgaggt tagtcgcgta gctcgcactc
     6061 caatccgaaa attaggctat aatgaacgat tcatccggcc gatacgtgaa ttgaaagaac
     6121 tcagtttgtc atataaaaac ctacttaaaa cagttggcta tgcctttgac tatcgcgatg
     6181 taaatgatga agaaagtatt cgattaggtg aattgttggc taaacaatca gtcaaagatg
     6241 ttgttataca agttacaggt ttagacgacc aagaattgat tgagcaaatt gtagagtata
     6301 tttaatcttt ttcgaaaatc tcttcaaatc aggttagcat cgctttgtct taggcatatg
     6361 ttactgactt cgtcagttct atctacaacc tcaaagcagt gctttgagct gactccgtca
     6421 gtcttatctg caacctcaaa acactgtttg agttatctgc ggtaatcttt ctagcttgtc
     6481 tttgattttt gttgttattt ataaggtaaa agaagctgga caaaaagtct tcaaaatcgg
     6541 aaaaaggcag cctatcgggt gttcaaaaat cttgatagga tgtcctttat tatggaaagc
     6601 cttattggat tttctcctca gattgagttt ttgatcagct ttatgagata ggttttgcta
     6661 gagatgtagc ccatcatgtt atttttatgg acagtgggaa aattgttgaa aaaaataatg
     6721 cccatcaatt ctttagtcgt ccaagagaag aacgaaccaa gcaattttgg aacgaattct
     6781 ttcgaatgcg atctatatag taaaatgaaa caagaacagg acaaatcgat cgggacagtc
     6841 a
//
LOCUS       DBERDK010000090         6806 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.090, whole genome shotgun sequence.
ACCESSION   DBERDK010000090 DBERDK010000000
VERSION     DBERDK010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6806
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.090"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..315
                     /locus_tag="ACL73L_001883"
     CDS             <1..315
                     /locus_tag="ACL73L_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGS0772803.1"
                     /translation="ELIAPMTYEETMTSDFFEVWFQKFFLPTLTTPSVIIMDNARFHR
                     MGKLELLCEEFGHKLLPLPPYSPEYNPIEKTWAHIKKHLKKVLPSCNTFYEAFLSCSC
                     FN"
     gene            376..1338
                     /locus_tag="ACL73L_001884"
     CDS             376..1338
                     /locus_tag="ACL73L_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HGS0772804.1"
                     /translation="MNMSNITIDGQEMDLSQTMPSYAQVVSLYDDTSISVSNNETDKV
                     LAGSLYTDTNEQGLTIPSSLLKNWNEQTGKNLTANDLIGKSVSASIVESAAETSKIAQ
                     FQTKIVRVINDEDDMEDSNSFMLSHQMETILKEAGFTKAVSYFILELKDPSQTKVVTE
                     ELQKNKKYTVLSQQRVLDIVITFIRVIQGLLIVLSSQAIVVAAVMIGIIIYINIMQRS
                     KEIGVMKAVGYQNRDVKGIFIYEAIWIVGIALLLAFLVAQGVGSLANAIVSHFYPSIT
                     KVFELNLLSVLGTLVFALLLGYVSAYFPARKISKMDPIESLCYE"
     gene            1515..2522
                     /gene="gap"
                     /locus_tag="ACL73L_001885"
     CDS             1515..2522
                     /gene="gap"
                     /locus_tag="ACL73L_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HGS0772805.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFIKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHDVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
     gene            complement(2590..3465)
                     /locus_tag="ACL73L_001886"
     CDS             complement(2590..3465)
                     /locus_tag="ACL73L_001886"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HGS0772806.1"
                     /translation="MQFTFTLPASLPQMTVKQLLEEQLLIPRKIRHFLRIKKHILINQ
                     EEVHWNEIVNPGDVCQLTFDEEDYPQKTIPWGNPDLVQEVYQDQHLIIVNKPEGMKTH
                     GNQPNEIALLNHVSTYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISR
                     EYWALVDGNINKKELVFRDKIGRDRHDRRKRIVDAKNGQYAETHVSRLKQFSNKTSLA
                     HCKLKTGRTHQIRVHLLHHNLPILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLERLTF
                     TTLSNTFEKELKKNG"
     gene            3552..5747
                     /gene="pbp2a"
                     /locus_tag="ACL73L_001887"
     CDS             3552..5747
                     /gene="pbp2a"
                     /locus_tag="ACL73L_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP2A"
                     /protein_id="HGS0772807.1"
                     /translation="MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGP
                     IRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLQNMVAAGYI
                     DKNQETEAAEVDMTSQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQIVYENTSLFPRAEDGTSAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEAGWALNKQLDNHTMQYDSYTVDNYAGI
                     KTSREVPMYQALAESLNLPAVATVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIANHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYVMAGKTGTTEAVFNPEYTSDQWVIGYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTPGSTFTVENAYKQNGIAPANTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
     gene            5800..5952
                     /gene="rpmG"
                     /locus_tag="ACL73L_001888"
     CDS             5800..5952
                     /gene="rpmG"
                     /locus_tag="ACL73L_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HGS0772808.1"
                     /translation="MALKKASLACAVCGSRNYSIKISGNPKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            5962..6138
                     /gene="secE"
                     /locus_tag="ACL73L_001889"
     CDS             5962..6138
                     /gene="secE"
                     /locus_tag="ACL73L_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HGS0772809.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            6193..6729
                     /gene="nusG"
                     /locus_tag="ACL73L_001890"
     CDS             6193..6729
                     /gene="nusG"
                     /locus_tag="ACL73L_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HGS0772810.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTVQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAEL"
BASE COUNT         2120 a         1191 c         1471 g         2024 t
ORIGIN      
        1 gagttaatcg ctccaatgac ttacgaagag acgatgacga gcgacttttt tgaagtatgg
       61 tttcagaagt ttttcttacc aacattaacc acaccatcgg ttattattat ggataatgca
      121 agattccata gaatggggaa gctagaactc ttgtgtgaag agtttgggca taaactttta
      181 cctcttcctc cctactcacc tgagtacaat cctattgaga aaacatgggc tcatatcaaa
      241 aagcacctca aaaaggtatt accaagttgc aatacctttt acgaggcttt tttatcctgc
      301 tcttgtttca attgactata tatcacggat acagttggga aagaagctat taaatatttg
      361 gagagtcctt ttgggatgaa catgtcaaat attactatag atgggcaaga aatggatctg
      421 agtcaaacga tgccttctta tgctcaagta gtgagtcttt atgatgatac aagtatctct
      481 gttagtaata atgagacgga caaagtgttg gctggatccc tctatactga tacaaatgaa
      541 cagggattaa cgattccaag cagtttacta aaaaattgga atgaacagac aggaaaaaat
      601 ttgacagcta atgatcttat tggcaaatca gtctcagcca gcattgtaga aagtgctgcc
      661 gaaactagta agattgctca atttcaaacg aagattgtac gtgtaatcaa tgatgaagat
      721 gacatggagg acagcaacag tttcatgctg tctcatcaaa tggaaacgat tttgaaagag
      781 gctggattta cgaaagctgt atcttatttt atcttggaac tcaaagatcc atcacagaca
      841 aaagtagtaa cagaagaatt acagaaaaat aagaagtata ctgtgctttc tcaacagaga
      901 gttcttgata ttgtgattac ctttattcgt gttattcagg gattattgat tgtgctttca
      961 tcacaagcta ttgtggtagc agcggttatg attggtatca ttatttacat caatatcatg
     1021 caacgttcca aggaaatagg tgtcatgaaa gcagttggtt atcagaatcg tgatgtcaaa
     1081 ggaattttta tttacgaggc tatctggatt gtaggcatcg ccttgctgct ggcatttttg
     1141 gttgcacaag gggtgggaag tttggcgaat gcgattgtaa gtcactttta cccatccatc
     1201 actaaggttt ttgaattaaa tcttttatct gttttaggaa ctctagtttt cgctctatta
     1261 cttggttatg tctcagccta cttcccggcg cgtaagatta gtaaaatgga tcctatagaa
     1321 tcgttatgct atgaatagta agggattcct tggtttacag tcttaaaaag aaactaacat
     1381 agaaaaacaa tggccatact tgcaattcaa ctcaaatagt tgtataatag gtttgttgaa
     1441 aggtgatttg tagtgattca agttactctt ttcacaataa aaatttcatg attttcataa
     1501 ggaggaaatc actaatggta gttaaagttg gtattaacgg tttcggacgt atcggtcgtc
     1561 ttgctttccg tcgtatccaa aacgtagaag gtgttgaagt tacacgcatc aacgacctta
     1621 cagatccagt tatgcttgca cacttgttga aatacgacac aactcaaggt cgtttcgacg
     1681 gtactgttga agttaaagaa ggtggatttg aagttaacgg taaattcatc aaagtttctg
     1741 ctgaacgtga tccagaacaa atcgactggg ctactgacgg tgtagaaatc gttcttgaag
     1801 ctactggttt ctttgctaag aaagaagcag ctgaaaaaca ccttaaaggt ggagctaaaa
     1861 aagttgttat cactgctcct ggtggaaacg acgttaaaac agttgtattc aacactaacc
     1921 acgacgttct tgacggtact gaaacagtta tctcaggtgc ttcatgtact acaaactgct
     1981 tggctccaat ggctaaagct cttcaagaca actttggtgt tgttgaagga ttgatgacta
     2041 ctatccacgc ttacactggt gaccaaatga tccttgacgg accacaccgt ggtggtgacc
     2101 ttcgccgtgc tcgcgctggt gctgcaaaca tcgttcctaa ctcaactggt gctgcaaaag
     2161 ctatcggtct tgtaatccca gaattgaatg gtaaacttga tggatctgca caacgcgttc
     2221 caactccaac tggatcagtt actgaattgg tagcagttct tgaaaagaac gttactgttg
     2281 atgaagtgaa cgcagctatg aaagcagctt caaacgaatc atacggttac acagaagatc
     2341 caatcgtatc ttcagatatc gtaggtatgt cttacggttc attgtttgac gcaactcaaa
     2401 ctaaagttct tgacgttgac ggtaaacaat tggttaaagt tgtatcatgg tacgacaacg
     2461 aaatgtcata cactgcacaa cttgttcgta ctcttgaata cttcgcaaag attgctaaat
     2521 aattcttgag ttgatagaaa gcaaggcttt gtggtcttgc ttttttatat ggaaaaatgg
     2581 atgacacgat catccattct tttttaattc tttttcaaat gtatttgaaa gggtagtgaa
     2641 agttagcctc tctaaagtaa gtgggtgggt aaaggaaagt cggaaggcat gaagcataag
     2701 ccggcttgtc tttgatttac tattatagag agggtctccc aggataggaa gattatgatg
     2761 caaaaggtgc acacgaatct gatgggttcg ccctgtcttt agcttgcaat gagccaagga
     2821 agtcttgttt gagaattgct ttaatctgct tacatgcgtt tcagcatatt gcccattttt
     2881 tgcatcaact attctttttc tacgatcatg gcgatcacgt ccaattttgt ctctgaaaac
     2941 aagttctttt ttgttgatat ttccatcaac tagagcccaa tattctctag aaatctcttt
     3001 tttctccaat aagcgattga gaatgggcag gataaaagga tttttggcaa agagaactaa
     3061 gccactggtt tccatgtcca gacgatgaac gacatagcag gtttggccaa cataggtact
     3121 aacatggtta agaagggcaa tttcgtttgg ttgattacca tgcgttttca taccctctgg
     3181 tttgtttaca ataatcaagt gttgatcttg ataaacttcc tgcactaagt ctgggttgcc
     3241 ccaagggatc gtcttttggg gataatcttc ctcgtcaaaa gtcaactggc aaacatctcc
     3301 aggatttacg atttcgttcc agtggacttc ttcttgattt atcaaaatat gtttcttgat
     3361 tctcaaaaaa tgacggattt ttctagggat gaggagttgt tcctcaagta attgctttac
     3421 cgtcatttga ggtagagagg cgggtaatgt aaatgtgaat tgcatacaga tattgtaaca
     3481 aaaaaagccc tatttggata ggaaatagct aaattcttgt cttcctatga tgaagatgat
     3541 aaaataaacg catgaaatta gataaattat ttgagaaatt tctttctctt tttaaaaaag
     3601 aaacaagtga actagaggac tctgattcta ctatcttacg tcgctctcgt agtgatcgaa
     3661 aaaaattagc ccaagtaggt ccgattcgaa aattctggcg tcgttatcat ctaacaaaga
     3721 ttatccttat actaggtttg agtgcaggct tgctagttgg aatctatttg tttgctgtag
     3781 ccaagtcgac caatgtcaat gatttgcaaa atgccttgaa aactcggact cttatttttg
     3841 atcgtgaaga aaaagaggct ggtgccttgt ctggtcaaaa gggaacctat gttgagctga
     3901 ctgatatcag taaaaacttg cagaatgctg ttattgcgac agaagaccgt tctttctata
     3961 aaaatgacgg gattaactat ggccgtttct tcttggctat tgtcactgct ggacgttcag
     4021 gtggtggctc taccattacc caacagctgg ctaaaaacgc ctatttatca caagatcaaa
     4081 ctgttgagag aaaagcgaaa gaatttttcc ttgccttaga attaagcaaa aaatatagta
     4141 aggagcaaat tctaaccatg taccttaaca acgcttattt tggaaatggt gtgtggggtg
     4201 tagaagatgc gagtaagaaa tactttggag tttctgcatc agaagtgagt ctggatcaag
     4261 ctgcgactct ggcagggatg ctcaaggggc cggaactgta taatcccttg aattccgtag
     4321 aagattctac taatcggcgc gatactgtct tgcagaatat ggttgcagca ggatatattg
     4381 ataaaaacca agaaaccgaa gctgctgaag ttgatatgac ttcgcaattg cacgataagt
     4441 atgaaggaaa aatctcagat taccgttacc cctcttattt tgatgcggtg gttaatgaag
     4501 ctgtttccaa gtataatcta acagaggaag agattgtcaa taatggctac cgcatttaca
     4561 cagagctgga ccaaaactac caagcaaata tgcagattgt ttatgaaaac acatcgctat
     4621 ttccgagggc agaggatgga acgtctgctc aatcaggaag tgtagctctc gaaccgaaaa
     4681 cagggggagt tcgtggagtt gtcggtcaag ttgctgacaa tgataaaact ggattccgga
     4741 atttcaacta tgcaacccaa tcaaagcgta gtcctggttc tacaattaag cctttagttg
     4801 tttatacgcc agcagttgaa gcaggctggg ctttgaataa gcagttggat aaccatacca
     4861 tgcagtatga cagctatacg gttgataact atgcagggat caaaacgagt cgagaagttc
     4921 ctatgtatca agccttggca gaatcgctta atctacctgc tgttgccact gttaatgatt
     4981 tgggtgtcga caaggctttt gaggcaggcg aaaaattcgg actcaacatg gaaaaggtcg
     5041 accgtgttct tggtgtcgcc ttgggaagcg gtgttgaaac caaccctctt caaatggctc
     5101 aagcatacgc tgcctttgca aatgaaggtt taatgcctga agctcatttt attagtagaa
     5161 ttgaaaatgc tagtggacaa gttattgcga atcataaaaa ttcacaaaaa cgggtgattg
     5221 ataagtctgt agctgacaag atgaccagta tgatgttggg gactttcacc aacggtaccg
     5281 gtattagttc atcgcctgca gactatgtca tggcagggaa aactggaaca actgaagcag
     5341 ttttcaatcc ggagtacaca agtgaccagt gggtaattgg ttatactccg gatgtagtga
     5401 ttagccactg gcttggcttt ccgaccactg atgaaaatca ctatctagct ggctctactt
     5461 caaacggtgc agctcatgtc tttagaaaca ttgccaatac cattttacct tatacgccag
     5521 gaagtacctt tacggttgaa aatgcttata agcaaaatgg aattgcacca gccaatacaa
     5581 aaagacaagt acaaaccaat gataatagcc agacagatga taatttgtct gatattcgag
     5641 ggcgtgcgca aagtctagta gatgaggcta gccgggctat ctcagatgcg aagattaagg
     5701 aaaaggctca aacaatatgg gattcgatag tcaatctatt tcgctaagat gcttgtcaaa
     5761 gcctagcttt cttgttataa tggataagat ggaggcgtta tggcactaaa aaaagcaagc
     5821 ctagcttgtg cggtttgtgg ttcgagaaac tattcaatca agatcagcgg aaaccccaag
     5881 cctacacgac tagaagtaaa taaattttgt aagcattgtg gcaagtacac tacacacaga
     5941 gaaacgagat aggagagagc gatgcgtttt attggagata tttttagact tcttaaagac
     6001 acaacatggc caactcgcaa ggaaagctgg agagattttc gttctatcat ggaatacaca
     6061 gctttctttg tagtaattat ttacattttt gaccagttga ttgtttcagg tttgattcga
     6121 tttattaaca ttttttagaa gattagtgga gttaattaca ctagaaatct tctatttatg
     6181 aaaggaaata tcatggatag ttttgataaa ggatggtttg ttttacaaac ttattctggt
     6241 tatgaaaata aggtgaaaga aaatctatta caacgtgcac aaacctacaa tatgttggat
     6301 aatattctac gcgttgaaat tccaacacaa acagtgcaag ttgaaaaaaa tggaaagaga
     6361 aaagaagtag aagaaaatcg ctttccaggt tatgttcttg tagaaatggt catgacagat
     6421 gaagcttggt ttgttgttcg aaacacacca aatgttacag gatttgtcgg atcacacggg
     6481 aacagatcaa aaccaactcc attattggaa caagaaattc gtgacatttt ggtatctatg
     6541 ggacaaactg ttcaagaatt tgatttcgat gttgagattg gtcaaactgt acgtattatt
     6601 gatggtgctt ttgcagacta cactggtaag attacagaaa ttgataataa caaagtgaaa
     6661 atgattatct ctatgtttgg taatgacaca gttgcagaag taaacctaaa ccaaatcgca
     6721 gaattataat cctgagagag gctggagcct ctcttttttg tgcagtttag gagctaaagg
     6781 gaacagaatg gagaaaatgg aacaaa
//
LOCUS       DBERDK010000091         6755 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.091, whole genome shotgun sequence.
ACCESSION   DBERDK010000091 DBERDK010000000
VERSION     DBERDK010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6755
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.091"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(373..1365)
                     /locus_tag="ACL73L_001891"
     CDS             complement(373..1365)
                     /locus_tag="ACL73L_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGS0772811.1"
                     /translation="MSYYDFVKDYMKVDECKNNEKYSSAGHTFCWKKENSTYAEGLYW
                     FYEGGSFIIDIHDFYIKEEVVQNSTYSMSDYVSIYSSYIVSANGEKFNPYQTITANSL
                     CTFDFDNIKDDFVFLLHENCCYLAVSIGFKKELIEKHLASININPESFYSTLLQPNQI
                     ILTKALERVAMEILNCKMDAPAADFFFKAKANEWISIVIDTYLNRKKYKIEIDDDKAL
                     EDVARFLDDHFATNVNQGTLEKISKMSGTKLKNLFKEKYCQSITEYTQRKRMNVAETL
                     LLNTELPIKEIAESVGYSSASKFSIYYKRYKGKLPSEVRNLACKHHNFKCDYCD"
     gene            1525..3279
                     /locus_tag="ACL73L_001892"
     CDS             1525..3279
                     /locus_tag="ACL73L_001892"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0772812.1"
                     /translation="MKIYKKLFAYVQDKKYLGVLAIIFSAISALLTVYGYYLIYKFLD
                     KLIINSNLSGAESIALKSVITLTSGAIFYFVSGMFSHILGFRLETNLRKRGIDGLEKA
                     SFRFFDLNPSGQIRKIIDDNAAQTHQVVAHMIPDSSQAIITPVLVLALGFIVSIRVGI
                     ILLALTIIGGLILGAMMGEQEFMKIYQESLSKLSAETVEYVRGMQVVKIFKANVESFK
                     SFYKAIKDYSKYAYDYSLSCKRPYVLYQWLFFGLIAILIIPIVYFMTSLASAKVILLE
                     LIMILFLSGVLFVSFMRMMWYSMYISQGNYAVDTLEALYEDMQKDKLVHGNINNFKNY
                     NIEFENVSFAYNDKAVIENLSFNLEEGKSYALVGSSGSGKSTVAKLISGFYNVNKGSI
                     KIGGIAISEYSDEALIKAISFVFQDSKLFKKSIYDNVALANKDATKDDVMRALKLAGC
                     DLILDKFPERENTIIGSKGVYLSGGEKQRIAIARAILKDSKIIIMDEASASIDPDNEF
                     ELQKAFKNLMKDKTVIMIAHRLSTIKDLDEIIVMDSGKIIERGSDKELMSKDTRYKSL
                     QEMFNSANEWRVSNERVL"
     gene            3263..4984
                     /locus_tag="ACL73L_001893"
     CDS             3263..4984
                     /locus_tag="ACL73L_001893"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0772813.1"
                     /translation="MKEFYKKRFALTDGGARNLSKATLASFFVYCINMLPAILLMIFA
                     QEVLENMGKSNGFYIVFSVLTLIAMYILLSIEYDKLYNTTYQESADLRIRTAENLSKL
                     PLSYFSKHDISDISQTIMADIEGIEHAMSHSIPKVGGMVLFFPLISVMMLAGNVKMGL
                     AVIIPSILSFIFIPLSKKYQVNGQNRYYDVLRKNSESFQENIEMQMEIKAYNLSKDIK
                     DDLYKKMEDSERVHLKAEVTTILTLSISSIFSFISLAVVIFVGVNLIINKEINSLYLI
                     GYLLAAMKIKDSLDASKEGLMEIFYLSPKIERLKEIQNQDLQEGDDYSLKKFDIDLKD
                     VEFAYNKDAKVLNGVSFKAKQGEVTALVGASGCGKTTILKLISRLYDYDKGQILIDGK
                     DIKEISTESLFDKVSIVFQDVVLFNQSVMENIRIGKQDASDEEVKRAAKLANCTDFIE
                     KMDKGFDTVIGENGAELSGGERQRLSIARAFLKDAPILILDEITASLDVNNEKKIQES
                     LNNLVKDKTVVIISHRMKSIENADKIVVLQNGRVESEGKHEELLQKSKIYKNLIEKTK
                     MAEEFIY"
     gene            4995..5624
                     /locus_tag="ACL73L_001894"
     CDS             4995..5624
                     /locus_tag="ACL73L_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HGS0772814.1"
                     /translation="MDNKKLKVKDLVSIGVFGVIYFAFMFGVGMMGLIPILFLIYPTV
                     LAIVAGTVVMLFMAKVQKPWALFIFGMISPLVMFAAGHTYVVVVLSLIVMIIAELIRK
                     IGNYNSFKYNMLSYAIFSTWICSSLMQMLLAKEKYMEWSLMTMGKDYVDVLEKLITYP
                     HMALVALGAFLGGILGAYIGKALLKKHFSNGLYCVGYFTPCLILWCYLN"
     gene            5525..6082
                     /locus_tag="ACL73L_001895"
     CDS             5525..6082
                     /locus_tag="ACL73L_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001851583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HGS0772815.1"
                     /translation="MYRQGSIEKTLFKWIILCGILYSLPNFMVLSELNPIVKMFLSIP
                     IVIRMFILPFMAASFMIKTSDVGAIISSMDKLKISKNVSIPIAVMFRFFPSFKEEKKN
                     IKMAMRVRGINFKNPVKYLEYVSVPLLIISSNISDDIAKAAETKAIENPIAKTRYIRV
                     KIQLIDFVYVLAVAGLIVGGLIWLK"
     gene            6070..>6755
                     /locus_tag="ACL73L_001896"
     CDS             6070..>6755
                     /locus_tag="ACL73L_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0772816.1"
                     /translation="MVEIKNLSLDYGEEHILDDISLSIAEGECVLFTGKSGSGKSSLI
                     NSINGLAVRYDNAKTKGEIIIDGKNIKNLELYQISMLVSTVFQNPKTYFFNVNTTLEL
                     LFYLENIGLAREEMDRRLKDMLEIFPIKNLLNRNIFNLSGGEKQILCIAASYIAGTKI
                     IVMDESSSNLDIKSISVLAKMLKILKEKGISIIVAEHRIYYLMDIVDRVFLIDKGKLK
                     KTYTRSEFLKL"
BASE COUNT         2413 a          920 c         1188 g         2234 t
ORIGIN      
        1 tttataatat cactaaatcc acctaattat aggacgtttt cagattttta gtcccgtccc
       61 agtcccagta ccggagaaat attgttttaa tataatatct ctttttgtct tctaagctct
      121 taaaagcaaa agaacaagta aagagtcaag acaaggataa aaagtccata ttagggcaaa
      181 taaaaagctt taagacagat gacaaatcta agtcaaataa gaaagaccat agcaaaggtg
      241 cagagagata aatattggcg gtcttcggac tgcctttatt tttttatcca tttttcaaat
      301 caaatttatt cagactatat atgcacatat acacttaaat tcatataaaa acatggcttg
      361 taaaaaatta ctttaatcac aataatcgca tttaaaattg tgatgtttgc aagctaaatt
      421 acggacttca cttggaagtt ttcccttgta tcttttataa tagatagaaa atttgctggc
      481 agatgaatat ccaacagatt ctgctatctc ttttataggt agttcagtgt ttaaaagaag
      541 agtttcagct acattcattc tttttctttg agtgtactct gtaatgcttt gacaatattt
      601 ttccttaaat aaatttttta atttagtacc actcatttta gatatttttt caagcgtgcc
      661 ttgatttaca ttcgttgcaa aatgatcatc taagaatctt gctacatctt caagtgcttt
      721 atcatcatca atttcaattt tatatttttt tctatttaag tatgtgtcaa ttactatact
      781 tatccattca tttgcctttg ctttaaagaa aaaatcagcg gcaggagcgt ccatcttaca
      841 atttaatatt tccattgcca ctctttctaa ggcctttgta agtattattt gattcggttg
      901 aagcaaggtt gaataaaaag attctggatt aatgttaata gatgctaaat gtttttctat
      961 tagctctttt ttaaaaccaa tggaaacagc aagataacaa caattctcgt gtaataaaaa
     1021 aacaaaatca tcttttatat tatcaaaatc aaaagtacat aaagagtttg cggtaatagt
     1081 ttgatacgga ttaaactttt ctccgtttgc actgacaatg taacttgaat aaattgaaac
     1141 atagtctgac atactataag tgctattttg aactacttcc tctttgatat aaaaatcatg
     1201 tatatcgata atgaagctgc ctccttcata aaaccaatat aggccttctg cataagttga
     1261 attctctttt ttccaacaaa atgtatgacc tgcacttgaa tacttctcat tgtttttaca
     1321 ttcatctact ttcatataat cttttacaaa atcataatat gacataacac actatccctt
     1381 ttagacaata ttccaattag ccttattaat tcaaaactat tgtattagta attataacag
     1441 atgtataata gaaaagcaat gatagatatt atcaattaag cgaatttata tctaaaaggg
     1501 atattaaaga aaggagatat gcttatgaag atttacaaaa aactatttgc ttatgtccaa
     1561 gataagaaat atcttggggt tttggccata attttttctg ctatatctgc tctacttaca
     1621 gtatatggat attatttaat ctacaaattt ctagataagt taataattaa ttcaaattta
     1681 tccggtgcag agagtatagc attaaaatct gttattacac taacaagtgg agcgatattt
     1741 tattttgtct caggaatgtt ttcacacatc ttgggattta ggcttgaaac aaatttaaga
     1801 aaaaggggaa ttgatggctt agagaaagca agttttaggt tctttgactt aaatccatct
     1861 ggtcaaataa gaaagatcat agatgataat gcagcacaaa ctcatcaggt ggtagcacac
     1921 atgattcccg atagttctca ggcaataatc acacccgtac ttgtacttgc acttggcttt
     1981 atagtaagta taagagttgg cataattttg cttgctctta ctataattgg cggcttaatt
     2041 ttaggggcaa tgatgggcga gcaagaattt atgaagatat accaagaatc cctatctaaa
     2101 ctaagtgctg aaactgttga gtacgtgaga ggaatgcaag ttgtaaaaat atttaaagca
     2161 aatgtagagt cttttaaaag cttttataag gcgataaaag attactcaaa gtatgcttat
     2221 gattattccc tatcttgtaa aaggccttat gttttgtatc aatggttatt ttttggactg
     2281 attgcaattt taattattcc tatagtttat tttatgacta gcttagctag cgcaaaggtg
     2341 attttacttg agcttatcat gattttattt ttatcaggag ttctctttgt ttcattcatg
     2401 agaatgatgt ggtactccat gtatatttct caaggaaatt atgcagtaga tactttagag
     2461 gcgctttacg aagatatgca aaaagacaaa ttagtgcatg gtaatatcaa taattttaaa
     2521 aactataata tagaatttga gaatgttagc tttgcttata atgataaagc tgtcattgaa
     2581 aatttatcct ttaatttaga agaaggaaag tcctacgcac ttgtcggttc atctggatca
     2641 ggcaaatcaa cagtagcaaa acttatatca ggtttttata atgttaataa aggaagcata
     2701 aagataggcg ggatagcaat aagtgaatat tctgacgaag ccttaattaa agccatttcc
     2761 tttgtttttc aagattcaaa attattcaag aagagcattt atgataatgt agctttagct
     2821 aataaagatg cgacgaaaga tgacgttatg agagccttaa aattagcagg atgcgattta
     2881 atattagaca aattcccaga aagagaaaat acaatcatag gctcaaaagg tgtttattta
     2941 tccggtggag aaaaacaaag aattgcaatt gctagagcaa ttttaaagga ttccaaaatt
     3001 attattatgg atgaagcatc agcatctatt gacccagata acgagtttga attgcaaaaa
     3061 gcttttaaaa atcttatgaa ggataaaaca gttatcatga ttgcacacag gctatctaca
     3121 attaaagacc ttgatgaaat tattgtcatg gatagtggaa aaattataga aagagggtct
     3181 gacaaagaat taatgtcaaa agatacaagg tataagagcc tgcaagagat gtttaacagt
     3241 gcgaatgaat ggagggtttc aaatgaaaga gttttataaa aaaagatttg ctcttacaga
     3301 tggaggagca agaaatttaa gtaaagcaac actggcttca tttttcgttt attgtataaa
     3361 catgcttcct gccatattac ttatgatttt tgctcaggaa gttttggaaa atatgggcaa
     3421 aagcaatggc ttttatatag tattctcagt tttgactttg atagcaatgt atattttgct
     3481 ttctatcgaa tacgataaat tatataacac aacctatcaa gaaagtgcag atttaagaat
     3541 aaggacagcg gagaatttat caaaattacc tctatcttac ttttctaaac atgacatttc
     3601 cgacatttca caaacaatca tggctgatat tgaaggcata gagcatgcaa tgagccactc
     3661 aataccaaag gtgggcggca tggtactgtt tttcccatta atatctgtaa tgatgctagc
     3721 gggcaatgtc aagatgggtt tagctgtaat tatcccatct attttaagct ttatatttat
     3781 acctttatct aaaaaatatc aggttaatgg acagaataga tattatgatg tcttaagaaa
     3841 aaactcagaa agctttcaag aaaatatcga aatgcaaatg gagattaaag catataattt
     3901 atcgaaggat attaaagatg acttatataa aaaaatggaa gatagtgaga gagtacactt
     3961 aaaggcggaa gtaactacaa ttttaacttt gtctatatct tcaatattta gctttatatc
     4021 tcttgctgtt gtgatatttg tcggcgtaaa tctaattatt aataaagaga taaattctct
     4081 ctaccttata ggatatttac tagctgctat gaagataaaa gactctttag atgcatctaa
     4141 agagggcttg atggaaatat tttatttatc gcccaaaata gaaagattaa aagaaattca
     4201 aaatcaagat ttacaagaag gcgatgacta tagcttaaaa aaatttgata ttgatctaaa
     4261 agatgttgag tttgcctaca ataaagacgc aaaagtttta aatggtgtaa gttttaaagc
     4321 taagcaagga gaggtcactg ctttggtagg tgcaagtggc tgcggtaaaa caactatctt
     4381 gaaacttata tcaagacttt atgattatga caagggacaa atcttaatcg atggcaaaga
     4441 tataaaagaa atatcaacag aatctctttt tgataaggtc tctatagttt tccaagatgt
     4501 ggttctcttt aatcaaagcg ttatggaaaa tattagaatc ggaaagcaag atgcaagtga
     4561 cgaagaggtt aaaagagcag caaaacttgc aaattgcaca gattttatag aaaaaatgga
     4621 taaaggtttc gatacagtta ttggtgagaa cggagctgag ctatcaggag gagaaagaca
     4681 aagattatca atagccagag ccttcttaaa agatgcgccg atattgatct tagatgagat
     4741 aacagcaagc cttgatgtta acaacgagaa aaagattcaa gagtctttaa ataatttagt
     4801 taaagataaa actgttgtaa tcatttcaca tagaatgaaa tccatagaaa atgcagacaa
     4861 gatagtagtt cttcaaaacg gaagagtaga aagcgaaggt aagcatgaag agcttttaca
     4921 aaaatcaaaa atttacaaaa atttaataga aaagacaaaa atggcagaag aatttattta
     4981 ttaggaggac tacaatggat aataaaaaat taaaagtaaa agatttagta agcatcggtg
     5041 tttttggcgt aatttatttt gccttcatgt ttggagttgg tatgatgggc ttgattccaa
     5101 tattgttctt aatatacccg acagtattag ccatagttgc aggaactgtt gttatgttat
     5161 ttatggctaa ggttcaaaag ccatgggcac tatttatatt tggtatgata tcaccacttg
     5221 tgatgtttgc agctggtcat acctacgtag ttgtggtttt atcacttata gtaatgataa
     5281 tagcagaatt aattagaaag attggtaatt ataattcatt taaatacaat atgctttctt
     5341 atgcaatctt cagcacatgg atatgtagct ctttaatgca aatgctttta gcaaaagaaa
     5401 aatatatgga gtggtctttg atgacgatgg gaaaagatta tgttgatgtg ttagaaaagt
     5461 taataactta tcctcatatg gctttagtag ccttaggtgc tttcttagga ggaattcttg
     5521 gagcatatat aggcaaggct ctattgaaaa aacacttttc aaatggatta tattgtgtgg
     5581 gatactttac tccttgccta attttatggt gctatctgaa ttaaacccta tagttaagat
     5641 gtttttgagt atacctattg ttattagaat gtttatttta ccatttatgg cagcaagctt
     5701 tatgataaag acctcggatg taggcgcaat aatttcatcg atggataagc ttaagatttc
     5761 aaagaatgta tccataccta ttgcggttat gtttagattc ttcccatctt ttaaggagga
     5821 gaagaaaaac atcaaaatgg ctatgagagt aagagggata aattttaaaa acccagtcaa
     5881 atatcttgaa tatgtttctg tgccactact cattatatca tctaatatat cagatgacat
     5941 tgcaaaagcg gcagaaacaa aggcaataga aaatccaatt gccaagacca gatacattcg
     6001 cgtaaagata cagctaattg attttgttta tgttttagcg gttgctggac ttattgtggg
     6061 aggcttaata tggttgaaat aaaaaattta agtcttgatt atggtgaaga gcatatatta
     6121 gatgatatat cactatccat agccgaggga gagtgcgtgc tatttacagg aaaaagtgga
     6181 agtggtaagt catctttaat aaattcaatc aatggactag ctgtaaggta tgataacgca
     6241 aagacaaagg gcgaaataat tattgatggt aagaatataa aaaatttgga actttatcaa
     6301 atctcaatgc ttgtttcaac tgtttttcaa aatcctaaga catatttttt taatgtcaat
     6361 acgacattag aattattatt ttatttggaa aatatcggtc ttgcaagaga agagatggac
     6421 aggcgtttga aggatatgct tgagatattc ccgataaaaa atcttttgaa cagaaatata
     6481 tttaatctat ccggcggtga aaaacaaatt ctttgcattg cagcttctta tatagcaggt
     6541 acaaagatta tagttatgga tgagtcttca tcgaatttag atattaaaag cataagtgtt
     6601 ttggcaaaga tgctaaagat attaaaagag aaaggcataa gcataattgt tgcagagcat
     6661 agaatttatt atttgatgga catagttgac cgtgtatttt taatagataa aggaaagctt
     6721 aaaaaaactt atactagaag tgaattttta aagct
//
LOCUS       DBERDK010000092         6248 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.092, whole genome shotgun sequence.
ACCESSION   DBERDK010000092 DBERDK010000000
VERSION     DBERDK010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6248
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.092"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            1..125
                     /locus_tag="ACL73L_001897"
                     /pseudo
     CDS             1..125
                     /locus_tag="ACL73L_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            278..478
                     /locus_tag="ACL73L_001898"
     CDS             278..478
                     /locus_tag="ACL73L_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772817.1"
                     /translation="MVVSASASSTSTQAQEQVDKSELRALSQELDQRLKALATVSDPK
                     IDATKAVLLDAQKAPEDSALTE"
     gene            503..920
                     /locus_tag="ACL73L_001899"
                     /pseudo
     CDS             503..920
                     /locus_tag="ACL73L_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642281.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transmembrane domain-containing protein"
     gene            1151..1858
                     /gene="ccdA2"
                     /locus_tag="ACL73L_001900"
     CDS             1151..1858
                     /gene="ccdA2"
                     /locus_tag="ACL73L_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HGS0772818.1"
                     /translation="METIVFLISVFLAGVLSFFSPCIFPLLPVYAGILLDDQERAKSF
                     SLFGRKVLWSGLIRTLCFIAGISLIFFILGFGAGYFGHILYANWFRYVMGAIIIILGL
                     HQMEIFHLKKLEVQKSFTFKKSDSNRYWSAFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMALPFLVLALASGLVMPYFSKIKRHMMLLKKIGGFLIVLM
                     GILLLLGQVNVLAGIFE"
     gene            1869..2426
                     /gene="sdbB"
                     /locus_tag="ACL73L_001901"
     CDS             1869..2426
                     /gene="sdbB"
                     /locus_tag="ACL73L_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
                     /protein_id="HGS0772819.1"
                     /translation="MKKVMFAGLSLLSLVVLMACGEEETKKTQAAQQPKQQTTVQQIS
                     VGKDVPDFTLQSMDGKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDF
                     EILTVIAPGIQGEKTVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDS
                     QGKIGKIQFGAISNADAEAAFKEMN"
     gene            2800..4191
                     /locus_tag="ACL73L_001902"
     CDS             2800..4191
                     /locus_tag="ACL73L_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HGS0772820.1"
                     /translation="MNIFRTKNVSLDKTEMHRHLKLWDLILLGIGAMVGTGVFTITGT
                     AAATLAGPALVISIVISALCVGLSALFFAEFASRVPATGGAYSYLYAILGEFPAWLAG
                     WLTMMEFMTAISGVASGWAAYFKGLLSQYGIAFPQALNGTFNPQAGTFVDLLPILVLV
                     LVTSLVLLNAKAALRFNSILVILKFSALALFVLVGIWNIKFDNWSNFAPYGFGQIYGA
                     STGIMAGASLMFFGFLGFESISMAVDEVKTPQKNIPRGIVLSLSIVTILYALVTLVLT
                     GVVHYSHLNVDDAVAFALRSVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLAR
                     DGLVPAAFKELTKTSKIPKNATILTGLASAVAAGMFPLASIAAFLNICTLAYLIMLAY
                     GLIRLRKEKGMPKAGEFKTPLVPLLPILSIIICLSFMLQYNMNTWIAFLVALLVGSII
                     YFTYGYKHSTIEE"
     gene            4334..5251
                     /gene="adcAII"
                     /locus_tag="ACL73L_001903"
     CDS             4334..5251
                     /gene="adcAII"
                     /locus_tag="ACL73L_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HGS0772821.1"
                     /translation="MKKQNLFLVLLSVFLLCLGACGQKESQTGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDADVFVYHSHTLESWAGSLDPNLK
                     KSKVKVLEASEGMTLERVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDSEHKETYQKNAQAFIKKAQELTKKFQPKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLTEIQEFVKTYKVKTIFTESNASSKVAETLVKSTGVGLKTL
                     NPLESDPQNDKTYLENLEENMSILAEELK"
     gene            5259..>6248
                     /gene="phtB"
                     /locus_tag="ACL73L_001904"
     CDS             5259..>6248
                     /gene="phtB"
                     /locus_tag="ACL73L_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtB"
                     /protein_id="HGS0772822.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRHQAGQVKKESNRVSYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVIKVNGKYYVYLKDAAHADNIRTKEEIKRQKQER
                     SHNHNSRADNAVAAARAQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKNEL
                     SASELAAAEAYWNGKQGSRPSSSSSYNANTAQPRLSENHNLTVTPTYHQNQGENISSL
                     LRELYAKPLSERHVESDGLIFDPAQITSRTARGVAVPHGNHYHFIPYEQMSELE"
BASE COUNT         1908 a         1135 c         1316 g         1889 t
ORIGIN      
        1 gtctaacaaa tggtgagtta atcgttccaa tgacttacga agagacgatg acgagcgact
       61 tttttgaagc ttggtttcag aagtttttct taccaacatt aaacacacca tcggttatta
      121 tagtaaaatg aaataagaac aggacaatca aatcaatttc tagcaatgtt ttagaagtcc
      181 atatgtacta ttctagtttc aatataccat aactgctcct agtcaacctg caagtcctgt
      241 cccagctcca agctcagctc cgactcaaga tcctagagtg gtagtaagcg cttcggctag
      301 ctcaacgagc acacaagctc aagagcaagt tgacaagtct gaacttcgtg ccttgagtca
      361 agagttagac caacgcttga aagctttggc aacagtatct gatccgaaaa tcgatgcaac
      421 caaggctgtc ctcctagatg ctcaaaaagc tccggaagat agtgccttga cagagtaagg
      481 tctgcatact gcagtagagt ctgtgaagac tgccttggac tctctcaaag atgtgatggc
      541 aaatgctagc gacagcaagc ctacacagga caagaaggat gcaaaacaag gaacggaaga
      601 tagtaaggat tcagataaga tgactgaaac aaactcagtt ccagcaggag tgattgtggt
      661 cagtctactt gccctcctag gcgtgattgc cttctggcta attcgccgta agaaagagtc
      721 agaaatccag caattaagca cggaattgac caaggttcta ggacagctag atgcagaaaa
      781 aagcggataa aaaagtcctt gccaaagccc aaaaccttct ccaagaaacc cttgatttcg
      841 tgaaataaga aaatggctca gcagagacag aaactaaact agtagaggag cttaaagcaa
      901 tccttgacaa actcaagtaa aacatgattt atgaaatgtc agacctaaaa gacattttcg
      961 ttcacaaaag cagcgacgag taatccttgt cgttgctttt tggctatttt gggggataag
     1021 atccgccttg actagcaaaa gaaaagattg gtccaatagt gcccgatacg attttgcgta
     1081 gcagaaagct tacaaataaa attgaatata gtaaaatagg attagaatta ttaagaaagt
     1141 tggttcttta ttggaaacga tagtattttt aatctctgtt tttctagcag gtgttttatc
     1201 ctttttttct ccttgtattt ttcctcttct gccagtctat gctgggattt tattggatga
     1261 tcaagaaagg gcaaaaagct tttctttgtt tgggagaaag gttctctggt caggcttgat
     1321 tcgaacactt tgctttatcg ctggtatctc tctcattttc tttattctag gctttggtgc
     1381 tggttacttt ggtcatattc tctatgcaaa ttggtttcga tatgtcatgg gagctattat
     1441 tatcattttg ggtcttcacc agatggaaat ttttcatttg aagaaattag aagttcaaaa
     1501 aagttttacc tttaaaaaat cagattctaa tcgttattgg tcagcttttt tacttggtat
     1561 tacctttagc tttggttgga caccttgtat tggtccagtt ttaagttctg ttttagcact
     1621 tgcggcttct ggaggcaatg gtgcttggca aggagcgatt tatactctca tttacactct
     1681 gggcatggcc cttcctttct tggtcttggc actagcttca ggtctagtca tgccatattt
     1741 tagtaaaatc aagcgtcata tgatgctact aaagaaaatt ggtggtttcc tcattgtttt
     1801 aatgggaatt ttgttactat taggacaagt aaatgttcta gctggaattt ttgaataaag
     1861 gaggacaaat gaaaaaagta atgtttgctg gcttaagtct cttgtcatta gttgtattga
     1921 tggcctgtgg tgaggaagaa actaaaaaga ctcaagcagc acaacagcca aaacaacaaa
     1981 cgactgtaca acaaatttct gttggaaaag atgttccaga ctttacattg caatccatgg
     2041 atggcaaaga agttaagtta tctgatttta agggtaaaaa ggtttacttg aagttttggg
     2101 cttcatggtg tggtccatgc aagaaaagta tgccagagtt gatggaacta gcggcgaaac
     2161 cagatcgtga tttcgaaatt cttactgtca ttgcaccagg aattcaaggt gaaaaaactg
     2221 ttgagcaatt cccacaatgg ttccaagaac aaggatataa ggatatccca gttctttatg
     2281 ataccaaagc aaccaccttc caagcttatc aaattcgaag cattcctaca gaatatttaa
     2341 ttgatagcca aggaaagatt ggaaagattc aatttggtgc tatcagtaat gcggatgcag
     2401 aagcagcatt taaagaaatg aactagaatc gataaaatct gattacaaga tgtagtcaga
     2461 ttttggtaac ttctaaaact aacttccagt ttcccacaac ctagctttta gtatgagaaa
     2521 aacgcgtgac atcagtggaa atccgtctta gactaacttc cactggtttt ctttttcttg
     2581 atatataagg gttcaaaagc gaaaagactc agaaaaagtg cacgacaaat agccctaaaa
     2641 cagagtcgtc ttagagtaac ttccagtttt gggagagaga tggaagttac tttgagaagt
     2701 tacggtagtc agattttttt agaaaaacat tttataaata ttcacatatc tcctatattt
     2761 atggtaaaat agaattatca gtttattttg gagtcaaaga tgaatatatt tagaacaaag
     2821 aatgttagtt tagataaaac agagatgcat aggcatttga agttatggga tttgattttg
     2881 ctgggtatcg gagccatggt agggacaggc gtctttacaa tcacaggtac tgcagctgca
     2941 acacttgctg gcccagccct agtgatttca atcgttattt ctgccttgtg tgtgggatta
     3001 tcagccctct tttttgcaga atttgcctcg cgagtacccg ctacaggagg tgcctatagt
     3061 tacctctatg ctatcttagg agaattccct gcctggttgg ctggttggtt aaccatgatg
     3121 gagttcatga cagccatatc aggcgtagct tcgggttggg cagcttattt taaaggcttg
     3181 ctctctcaat acgggatagc ctttcctcag gctttaaatg gtacctttaa tcctcaagca
     3241 gggacatttg ttgatttatt gcctattctt gtcttggtct tggtgacttc gcttgtttta
     3301 ctaaatgcca aggcagcctt acgctttaat tcgattctag ttattttgaa attttccgct
     3361 ttagctctct ttgttttagt aggaatttgg aatattaagt ttgataattg gagcaatttt
     3421 gctccttacg gttttggaca aatctatgga gctagtactg gtattatggc tggtgcatcc
     3481 ttgatgttct tcggtttttt gggatttgaa tccatctcta tggctgtaga tgaagtcaag
     3541 actcctcaaa aaaatattcc tagagggatt gtcttatcgc tttctatcgt aaccattctc
     3601 tatgccttgg tgacccttgt tttgactggt gtggttcact atagtcattt aaatgtcgat
     3661 gatgccgttg cctttgccct tcgtagtgtt gggattagtt gggcagccaa ctatgtgtca
     3721 ttagtggcta tcttgacctt gattacagtt tgtatttcga tgacctatgc cctatcgcgt
     3781 atgatttaca gtttagcacg tgacggctta gtgcctgctg cctttaagga actgacgaag
     3841 actagcaaga taccaaagaa tgctactatc ttgacaggtc tagcttcagc agtagcagca
     3901 ggaatgttcc cactagccag tattgcagcc tttttaaata tttgtacctt agcctacttg
     3961 atcatgttgg cttatggtct gattcgctta cggaaagaaa aaggaatgcc caaagctggg
     4021 gaatttaaaa caccattggt acctctgtta ccaattttat cgatcatcat ttgtttatcc
     4081 ttcatgttac agtataatat gaatacctgg attgctttcc tagttgcgtt gttagtagga
     4141 agtattattt acttcactta tggctataag cattctacca tagaagaata aagtgaaagt
     4201 ctattgagtt gttgacactc agcagatttt tatatgtgaa agaaatttca aattttttct
     4261 aaaaatatct tgacagatta aattttcagg agtagaatat ttactagtta attaaatggt
     4321 taaggagttg ttcatgaaga aacaaaattt atttttagtc ctgttaagtg tctttctttt
     4381 gtgcttgggg gcttgtggtc aaaaggaaag tcagacagga aaggggatga aaattgtgac
     4441 cagtttttat cctatctacg ctatggttaa ggaagtatct ggtgacttga atgatgttcg
     4501 gatgattcag tcaagtagtg gtattcactc ctttgaacct tcggcaaatg atatcgcagc
     4561 catctatgat gcagatgtct ttgtttacca ttctcataca ctcgaatctt gggcaggaag
     4621 tctggatcca aatctaaaaa aatccaaagt gaaggtctta gaggcttctg agggaatgac
     4681 cttggaacgt gtccctggac tagaggatgt ggaagcaggg gatggagttg atgaaaaaac
     4741 gctctatgac cctcacacat ggctagatcc tgaaaaagct ggagaagaag cccaaattat
     4801 cgctgataaa ctttcagagg tggatagtga gcataaagag acttatcaaa aaaatgcgca
     4861 agcctttatc aaaaaagctc aggaattgac taagaaattc caaccaaaat ttgaaaaagc
     4921 gactcagaaa acatttgtaa cacaacatac agccttttct tatctagcga agagatttgg
     4981 gcttaatcaa cttggtattg caggtatctc tcctgaacaa gaaccaagtc cacgacaact
     5041 aacagaaatt caggaatttg ttaagaccta taaggttaaa acgattttta cagaaagtaa
     5101 cgcttcttca aaagtagctg aaactcttgt caaatcaaca ggtgtgggtc ttaaaactct
     5161 gaatccttta gagtcagacc cacaaaatga caagacctat ttagaaaatc ttgaagaaaa
     5221 tatgagtatt ctagcagaag aattaaagtg aggaaagaat gaaaatcaat aaaaaatatc
     5281 tagcaggttc agtggcagtc cttgccctaa gtgtttgttc ctatgaactt ggtcgtcacc
     5341 aagctggtca ggttaagaaa gagtctaatc gagtttctta tatagatggt gatcaggctg
     5401 gtcaaaaggc agaaaatttg acaccagatg aagtcagtaa gagggagggg atcaacgccg
     5461 aacaaatcgt catcaagatt acggatcaag gttatgtgac ctctcatgga gaccattatc
     5521 attactataa tggcaaggtc ccttatgatg ccatcatcag tgaagagctc ctcatgaaag
     5581 atccgaatta tcagttgaag gattcagaca ttgtcaatga aatcaagggt ggttatgtca
     5641 ttaaggtaaa cggtaaatac tatgtttacc ttaaggatgc agctcatgcg gataatattc
     5701 ggacaaaaga agagattaaa cgtcagaagc aggaacgcag tcataatcat aactcaagag
     5761 cagataatgc tgttgctgca gccagagccc aaggacgtta tacaacggat gatgggtata
     5821 tcttcaatgc atctgatatc attgaggaca cgggtgatgc ttatatcgtt cctcacggcg
     5881 accattacca ttacattcct aagaatgagt tatcagctag cgagttagct gctgcagaag
     5941 cctattggaa tgggaagcag ggatctcgtc cttcttcaag ttctagttat aatgcaaata
     6001 cagctcaacc aagattgtca gagaaccaca atctgactgt cactccaact tatcatcaaa
     6061 atcaagggga aaacatttca agccttttac gtgaattgta tgctaaaccc ttatcagaac
     6121 gccatgtgga atctgatggc cttattttcg acccagcgca aatcacaagt cgaaccgcca
     6181 gaggtgtagc tgtccctcat ggtaaccatt accactttat cccttatgaa caaatgtctg
     6241 aattggaa
//
LOCUS       DBERDK010000093         6164 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.093, whole genome shotgun sequence.
ACCESSION   DBERDK010000093 DBERDK010000000
VERSION     DBERDK010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6164
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.093"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            95..781
                     /locus_tag="ACL73L_001905"
     CDS             95..781
                     /locus_tag="ACL73L_001905"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HGS0772823.1"
                     /translation="METKKIKNLKGQIIVSCQALEGEPLYTPNGGVMPLLAKAAFQAG
                     AKGIRANSVRDISEIKEEVDLPIIGIIKRDYDGFEPFISATMKEIDELVSEGVDILAL
                     DCTNRSRPGYDNITDFIHDIKVKYPNQLLMADISTFEEGKVAAESGVDFVGTTLSGYT
                     PYSPKKDNPDFELVERLVKELDVPVIAEGRISTPEQARKMLDLGAYAVVVGGAITRPL
                     EIAKKFIEVV"
     gene            782..1714
                     /locus_tag="ACL73L_001906"
     CDS             782..1714
                     /locus_tag="ACL73L_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS0772824.1"
                     /translation="MNQEILKKSKIQLTVEHIQKYWILYLMMIPATVLLILFTYGPMY
                     GIIMAFQDFTVFKGYTGSPFVGLKHFQRLFSDPLFYRLFKNTFMVGVLDFLFSFPAPL
                     IFALILNEVRKVRFKSVVQSISYLPHFIPLVVMVGIIFELFGSYGIINSLLSSLGMEP
                     ISFFTKSEWFLPLYIGSGVWKTIGWGSIIYMGALTNIDSTLYEAADMDGANRWHKMWH
                     VTLPSLRPTVVTLFILNAGGIMQVGFEKVFLMSSPATYEVSDVLSTYVYRQGILNSDF
                     SYSAAVGLFNNIVALLFVLLANKIAKKLGEEGIV"
     gene            1725..2654
                     /locus_tag="ACL73L_001907"
     CDS             1725..2654
                     /locus_tag="ACL73L_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGS0772825.1"
                     /translation="MIFSKNTKKSNRDKETFVDYLFIFILIIITLLMLYPLWWVVMTS
                     LSEPVWLSKNVVKLFPLKIDITAYKFIFGDSKLWQAYGNSVLYAALSTIVSVAVCSMY
                     AYPLTIPEIRGKKFWNTLILIPMFFSGGMIPLYIQVANLGLLNTAWALVLPGAVSTYY
                     IILFRTQFKSIPMELRESARIDGASEFRIFWNLVLPLSKVIFMIIALYCIVGSWNSFT
                     PPLLYLNDDTKMPLSIYLKNLVVTNTTDTAELSLSAGNTYYQQIAAQGGGIGMRIAIK
                     MGTIVVSVIPIMMIYPFIQKHFVKGILLGSVKG"
     gene            2668..4107
                     /locus_tag="ACL73L_001908"
     CDS             2668..4107
                     /locus_tag="ACL73L_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772826.1"
                     /translation="MQNLKKYLLLDSGLVVGYTLENAQLKLNKLKKDTENNPLFGEEY
                     FAENPKLWEVRFDNSYPNVIYDKELKLYRCWYQTFVSDEASEETPLAERGEKEYIVKS
                     SRMTALCYAESKDGVKWEKPNLNLVKFKGSKDNNIVLMHAHGAGIMLDEQELDPKKRY
                     KLVSKLEFSPNLHYMVVVFSEDGIHFDEPIPWKKWNPAADSHNFPFRDPKTGKYAVIT
                     RTWANNVRLSAITFSDDFINWSEPKEILRGDGFEDQIYSMPVISYADIYLGFASMYHE
                     GDTLNENYDKVDLELKFSANLEQWDSVCKGDYLIERGGGVYPQNADFDASCIFTAAPI
                     IEDDKIWFYYMGGNGNHTGFRESSLARGYIKKDQFAYYTNKREGIPAKISTVSFQTYD
                     DEIYILADIPENGYIKVAVGTKYGNAYSDFGQEDISLVQVSDGKYKLEFNKKSILDLG
                     NSPVAFKFEIENAKLYGIEGSIQQHYLKY"
     gene            4176..5753
                     /locus_tag="ACL73L_001909"
     CDS             4176..5753
                     /locus_tag="ACL73L_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HGS0772827.1"
                     /translation="MVKLNKVLKGSVAFLSASLLLAACGNNANSKPAEKTADGKTKIS
                     FLSTWQINDVRPPKDETLVGQWLEEQLGIDIDLVTIPGEGVSSKVNSLITSNQLPTVL
                     LTTGDKSDIAGINKLGKQGAFLDLSQHMDKLPNYKKYLEKNNALAQIEDDEKHIYAFT
                     KFFTDENIMYTTPILRKDLLVGSEFEDLSKIKTVDDYTKVLKYLTEKQGSPAFIQRNG
                     YEGFMKRVTPLWNLSHRTYYDYESDSYKHPVEQPQLKQFVEWLKELRKDNVLHPDWAV
                     MKDETWEGLLASNKGSFTVDRMNIIGDNNFDKPFDWQPLVYPEINGKRYLQPKTPYIS
                     DSGWVINANAPKEEIDKALEFFNFLYADENQEKLAIGFEGKTFTRENPNTQAGIKWLI
                     QIYGENAGNDKAELLFKHGNQAFTRVQTKLDIEAQPGTFPKVMYDQVDKIKKDLGGFR
                     PDTPVLSFTTEELDVLTKSEAGLNTFFDENIIKFIEGQRDMSEWDSFINEAKSKDLDK
                     VTKVYNDAYKRVKDRMK"
     gene            5813..>6164
                     /locus_tag="ACL73L_001910"
     CDS             5813..>6164
                     /locus_tag="ACL73L_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGS0772828.1"
                     /translation="MYDVVIIGAGSSGALAAIASGRLGLKTCLIEKGSRIGGVPINTL
                     MGSFANLYFDDSGRVNASKIVGELIERIIKKGGTVFNSMEELMNVNDFYQITIPYQPE
                     VYESVLTDMLIESKV"
BASE COUNT         2087 a          848 c         1189 g         2040 t
ORIGIN      
        1 gctgttcata tcttatttca attcactata taaataaaat tgtagtagat gttattgaat
       61 tagtaaaaaa aataaaaaaa cggaggtttt ctgaatggaa actaaaaaaa ttaaaaattt
      121 aaaaggtcaa ataattgttt cctgccaagc gttagaagga gagccacttt atactccaaa
      181 tggaggagtg atgccgttat tagcaaaagc cgcctttcaa gcaggcgcta aaggtattcg
      241 tgctaattct gtccgcgata tttcagaaat aaaagaagaa gttgacttac caattatagg
      301 tatcataaaa agagattacg atggctttga accttttatt tcagcaacta tgaaagagat
      361 tgatgaatta gttagtgaag gagtagacat tttagctttg gattgtacta atcgttcaag
      421 accaggttat gataatatta ctgactttat tcatgatatt aaagtcaagt atccaaacca
      481 attattaatg gctgatattt ctacttttga agaagggaaa gtagctgctg aatcgggagt
      541 agattttgtg ggtacgacat tatctggtta tacaccatat agccctaaaa aagataatcc
      601 tgattttgag ttagttgaaa gattggtaaa agagcttgac gtaccagtta ttgctgaagg
      661 aagaataagt actccggaac aagctagaaa aatgttagat ttaggtgctt acgcagtggt
      721 tgttggagga gctatcactc gtcctttgga aatagcgaag aagtttattg aggtagttta
      781 gatgaatcaa gaaatattaa aaaagagtaa gattcaattg acagttgaac atattcaaaa
      841 atattggatt ttatatttaa tgatgattcc agcaactgtt ctactaattt tatttacgta
      901 tggaccaatg tacggcatta ttatggcctt tcaagacttt actgtcttca agggatatac
      961 tggaagtcca tttgtcggtt taaaacattt tcaaagattg ttctcagatc ctttgtttta
     1021 tagattgttc aagaatacgt tcatggtagg ggttttagac tttttattct ctttcccagc
     1081 tccgcttatt tttgcactca tattaaacga agtcagaaaa gtaagattta agagtgtagt
     1141 tcaatctatt agttatcttc ctcattttat tcccctagtg gttatggttg gtattatttt
     1201 tgaactattt ggttcatatg gtattattaa cagtttactt tcatcacttg gaatggagcc
     1261 aataagtttc tttacaaaat ctgaatggtt cttgcctctc tacattggtt caggagtatg
     1321 gaaaacaata ggttggggtt ctatcattta tatgggagca ttaaccaata tagattcaac
     1381 tttatatgaa gcagcagata tggacggggc taatagatgg cacaaaatgt ggcatgtaac
     1441 tctaccaagt ttaagaccta cagtagttac tttgtttata ctaaatgctg gtggtatcat
     1501 gcaagtagga tttgaaaagg tattcttgat gtctagtcct gcaacatatg aagtatctga
     1561 tgtattatct acttatgttt atagacaagg tattttaaat tctgatttca gttactcagc
     1621 tgcagtagga ttatttaata acatagtagc tttgttattt gttttattag ctaataagat
     1681 tgctaaaaaa cttggcgaag aaggaattgt ataagggggg taaaatgatt ttttcaaaaa
     1741 atacaaaaaa gagtaataga gacaaagaaa ccttcgttga ttatctcttt atattcattt
     1801 taataataat aaccttactt atgttgtatc cattatggtg ggtagtgatg acgtcacttt
     1861 cagaaccagt ttggctatct aaaaacgttg ttaaattatt tcccttaaaa atagatatta
     1921 ctgcatataa gtttattttt ggggattcta aactatggca agcatatggt aatagtgtat
     1981 tgtatgctgc actatctaca attgttagtg ttgcggtatg tagtatgtat gcctatccac
     2041 ttacaattcc tgaaattagg ggtaaaaaat tctggaatac tcttattttg attccaatgt
     2101 tctttagtgg aggaatgatc ccgctttata tacaagttgc aaacctcggt ttattgaata
     2161 ctgcttgggc tcttgtttta cctggtgctg tttctacata ttatataatt ttgtttagaa
     2221 cacagtttaa atcaatccca atggagcttc gtgagtctgc tagaattgat ggggcaagtg
     2281 aatttagaat tttttggaat ttagtattac cattatcaaa agttattttc atgattatag
     2341 cactttattg catagttggt tcatggaata gttttactcc tccattactt tatttgaatg
     2401 acgacactaa gatgccttta tctatttatt tgaaaaattt agttgttaca aatacaactg
     2461 atactgctga attatcactt tctgcaggta atacatatta ccaacaaatt gctgctcaag
     2521 gtggaggtat tggtatgcgt attgctatta agatgggtac tattgttgtt agtgttattc
     2581 caattatgat gatttatcca tttattcaga aacattttgt taagggaatc ctgctaggtt
     2641 ctgtgaaagg ttaataaggt aattattttg caaaatttaa aaaaatatct tttattagat
     2701 agcggtttag tagttgggta tacattagaa aatgctcaat taaaattaaa taagctaaaa
     2761 aaagatactg aaaacaatcc actatttggt gaagagtatt ttgccgaaaa tcctaaatta
     2821 tgggaagttc gttttgataa ttcatatcca aacgttatct atgataaaga attgaaatta
     2881 tatagatgtt ggtatcaaac atttgttagt gatgaagcaa gcgaagagac tcctttagca
     2941 gaacgtggag agaaggaata cattgtcaag tcatctagaa tgacagcact ttgctatgct
     3001 gaaagtaaag atggagtaaa atgggaaaaa ccaaatctca atctagtaaa atttaaaggt
     3061 tcaaaagata ataatattgt tctgatgcat gcacacggtg caggtatcat gctagatgaa
     3121 caggagttgg atcctaagaa acgatataaa ttagtttcaa aattagaatt ttctccgaat
     3181 cttcattaca tggttgttgt tttttcagaa gacggtattc attttgacga acctattcca
     3241 tggaaaaaat ggaatcctgc agcagatagt cataattttc catttagaga tccaaaaact
     3301 ggtaaatacg ctgttattac aagaacctgg gcaaataacg tacgtctaag tgcaattact
     3361 ttcagtgatg attttattaa ttggtcagaa cctaaagaaa tccttagggg agatggattt
     3421 gaagatcaaa tttattctat gccagtaatt tcatacgcgg atatctattt aggttttgct
     3481 tcaatgtacc acgaaggcga cactttaaat gaaaactatg ataaagtaga cttagaattg
     3541 aaattctctg ctaatttgga acaatgggat agtgtatgta agggcgacta tctcattgaa
     3601 aggggaggtg gagtataccc gcagaatgct gattttgatg ctagttgtat tttcacggct
     3661 gctccaataa ttgaagatga taagatttgg ttctactata tgggtggaaa tggtaatcac
     3721 actggcttcc gagaaagttc attagctaga ggatatatta agaaagacca atttgcttat
     3781 tatactaata agagagaagg aattccagct aaaatatcta cagttagctt ccaaacttac
     3841 gatgatgaaa tttatatctt agctgatatt cctgaaaatg gttatattaa agttgcggta
     3901 ggtactaaat acggtaatgc ttattcagat tttggtcagg aagatatctc tcttgttcaa
     3961 gtttccgatg gtaagtataa gttggaattt aataaaaaat ctatcttaga tttaggtaat
     4021 tctccagttg cgttcaaatt tgaaatcgag aatgcaaaat tgtatggtat tgaaggtagt
     4081 attcagcaac actatctcaa atattaacca gaataattcg ttaacaaata atctctatat
     4141 tagtggagat agatatataa aatggaggtc ttattatggt aaagcttaat aaggtattaa
     4201 aagggagtgt tgcattcctg agtgcaagtt tattgctagc agcttgtggc aataacgcta
     4261 attcaaagcc cgctgaaaaa acagctgatg gtaaaacgaa aatttcgttt ctatctacat
     4321 ggcaaattaa tgatgttcgt ccgcctaaag atgaaacact tgttggtcaa tggttagaag
     4381 aacagctagg tattgatatt gatcttgtaa ctattcctgg cgagggagta agttctaaag
     4441 taaattctct tattactagt aaccagttac cgactgtttt gttgacaaca ggagataaga
     4501 gtgatatcgc tggtatcaac aaacttggta aacagggtgc attcttagat ttgtcacaac
     4561 acatggacaa actaccaaac tataagaagt accttgaaaa aaataatgca ttagctcaaa
     4621 tagaagatga tgaaaaacat atttatgctt tcacaaaatt ctttactgat gagaatatca
     4681 tgtatactac accaattctg agaaaagact tacttgttgg ttcagaattt gaagatcttt
     4741 caaaaattaa aactgttgat gactatacaa aagttttgaa atatttgact gaaaaacaag
     4801 gttctccagc atttattcaa cgtaatggct atgaaggctt catgaagcga gttacaccat
     4861 tgtggaattt aagccatcgt acatactatg actatgaatc agattcatat aaacatccag
     4921 ttgaacaacc tcaattgaaa caattcgttg aatggttgaa agaattacgt aaagataacg
     4981 tacttcaccc tgactgggca gttatgaaag atgaaacttg ggaaggtcta ttggcttcta
     5041 acaagggttc attcactgtt gaccgtatga atatcattgg agataataac tttgataaac
     5101 catttgattg gcagccactt gtttatccag aaattaatgg aaaacgttat ttgcaaccga
     5161 aaacaccgta catttctgat tcaggttggg taatcaatgc caatgcacca aaagaagaaa
     5221 ttgataaagc gttagaattc tttaatttct tatacgctga tgaaaatcaa gaaaaacttg
     5281 ctattggatt tgaaggtaaa acatttacac gtgaaaaccc taatactcaa gcaggtatta
     5341 aatggttgat tcaaatttac ggtgaaaatg ctggaaatga taaagcagaa ctgctgttta
     5401 aacatggtaa ccaagcattt actcgcgttc aaactaaatt agatattgag gcacagccag
     5461 gaacattccc taaagttatg tatgatcaag tagataaaat taagaaagat ttaggtggtt
     5521 ttagaccaga tactccggtt ctttccttta caactgaaga attagatgtt ttaactaaat
     5581 ctgaagcagg attaaatact ttctttgatg aaaatattat taaattcatt gaaggacaaa
     5641 gagatatgag tgagtgggat tcatttatca atgaagctaa atctaaagac ttggataaag
     5701 ttacaaaagt atataatgat gcctacaaac gtgtcaaaga tagaatgaaa tagactaaat
     5761 ggtagctctc tggtttttca gagagctact actgcctgag ggggagtaag ctttgtatga
     5821 tgtagtaatt ataggtgcag gaagttcggg cgcattggct gcaattgctt cggggcgtct
     5881 agggttaaaa acttgtctaa tagagaaggg aagtagaatt ggtggagttc ctattaatac
     5941 actaatgggg tcatttgcta atctatattt tgatgattct ggtagagtaa atgcaagtaa
     6001 aattgttggg gaattaatag aacgtattat caaaaaaggt ggtactgtct ttaactctat
     6061 ggaagaattg atgaatgtaa atgattttta tcagattaca attccctatc aaccagaagt
     6121 ttatgaatcg gttttaactg atatgttgat tgagtctaaa gttg
//
LOCUS       DBERDK010000094         6074 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.094, whole genome shotgun sequence.
ACCESSION   DBERDK010000094 DBERDK010000000
VERSION     DBERDK010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6074
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.094"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..651
                     /locus_tag="ACL73L_001911"
     CDS             <1..651
                     /locus_tag="ACL73L_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGS0772829.1"
                     /translation="INNVNIDNLDIPFEFKLTKEAQNLIIEHSDYIKAYYDKDITMED
                     YCKEKNNISFKATFGDIKWSLLNKQAHRNTVSGDFGCLSNTRKAQGRHLEQEGNIKHA
                     LIYYIESLIITISGLENNFSATDYPVYYPDSIPDYSLKHIQTLMESLSDDDYDFAFDE
                     ALFRFSILNANHFLSKEDIDYLRVNLPRSTAEEINNYLKKYECYSPLNNLELDDFE"
     gene            complement(818..889)
                     /locus_tag="ACL73L_001912"
     tRNA            complement(818..889)
                     /locus_tag="ACL73L_001912"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(854..856),aa:Arg,seq:cct)
     gene            complement(931..1278)
                     /gene="rplS"
                     /locus_tag="ACL73L_001913"
     CDS             complement(931..1278)
                     /gene="rplS"
                     /locus_tag="ACL73L_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HGS0772830.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVERIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            complement(1397..1726)
                     /gene="crcB"
                     /locus_tag="ACL73L_001914"
     CDS             complement(1397..1726)
                     /gene="crcB"
                     /locus_tag="ACL73L_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HGS0772831.1"
                     /translation="MVIVYLAIACGFGALVRYFFSRYNQASKLPLGTLIANLLGCFLI
                     GVFYNHVESKEVYAILATGFCGGLTTFSTLNDELQRLLSDKKVFYSYLTLTYIGGLVA
                     IFLGILL"
     gene            complement(1720..2094)
                     /gene="crcB"
                     /locus_tag="ACL73L_001915"
     CDS             complement(1720..2094)
                     /gene="crcB"
                     /locus_tag="ACL73L_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HGS0772832.1"
                     /translation="MKKEQFYPLGIFLAAMLGGLVRYLVSTWLPASPDFPWGTLFVNY
                     LGIFCLIFVVKGYLVYKGTSKGLILALGTGFCGGLTTFSSLMLDTVKLLDTGRYFSLV
                     LYLLLSIGGGLLLAYFLGRKKW"
     gene            complement(2091..2357)
                     /locus_tag="ACL73L_001916"
     CDS             complement(2091..2357)
                     /locus_tag="ACL73L_001916"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HGS0772833.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMHLVEGVVAYKKASGKPILD
                     TKREEVIFEKVRSRVEDKRYQETIVATFSDILKRSRDYQDQNIK"
     gene            complement(2473..2916)
                     /locus_tag="ACL73L_001917"
     CDS             complement(2473..2916)
                     /locus_tag="ACL73L_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HGS0772834.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRIFVSTGAEKGSECVKVDLSAEEEDIERLEQFAEELVAKVV"
     gene            3020..3955
                     /locus_tag="ACL73L_001918"
     CDS             3020..3955
                     /locus_tag="ACL73L_001918"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HGS0772835.1"
                     /translation="MEICQQILEKIKEYDTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIK
                     IDHHPNDDVYGDLSWVDTSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFL
                     YPSTTARTLRLAAYLREHNFDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVI
                     LSQEVLKQYNVTDAETAAIVGAPGRIDRVSLWGIFVEQADGHYRVRLRSKIHPINEIA
                     KEHDGGGHPLASGANSYSLEENEIIYQKLKNLLKN"
     gene            4051..4293
                     /locus_tag="ACL73L_001919"
     CDS             4051..4293
                     /locus_tag="ACL73L_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HGS0772836.1"
                     /translation="MKKDIHPEYRPVVFMDTTTGYQFLSGSTKRSNETVEFEGETYPL
                     IRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(4350..4928)
                     /locus_tag="ACL73L_001920"
     CDS             complement(4350..4928)
                     /locus_tag="ACL73L_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron permease"
                     /protein_id="HGS0772837.1"
                     /translation="MVRNYWKAKSWLLVLALSFLILSPAGAQESLSFFFVKITDASKA
                     VKNGGQTETQKLVTKMASDFERVENKDSEVGKIVKEKLALSGDITEAKLTEISSALLA
                     FEKEQNPVDLDAEKEKLVNRLSPRFETLEQAIASKDLEKVREAFKKMNSTWTINESVV
                     RDNSTAHYGRVETAISFLPSSMETEPTDESGT"
     gene            complement(4903..5914)
                     /locus_tag="ACL73L_001921"
                     /pseudo
     CDS             complement(4903..5914)
                     /locus_tag="ACL73L_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259617.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
     gene            complement(5917..6074)
                     /locus_tag="ACL73L_001922"
                     /pseudo
     CDS             complement(5917..6074)
                     /locus_tag="ACL73L_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         1922 a         1317 c         1055 g         1780 t
ORIGIN      
        1 attaataatg ttaacattga caatttagat attccgttcg aatttaaact aacaaaagaa
       61 gcacaaaatc ttattatcga acatagtgac tatatcaaag catactatga taaagacata
      121 actatggaag attattgtaa agaaaaaaac aatatctctt ttaaagcaac ttttggtgat
      181 ataaaatgga gtctcttaaa taaacaagct cataggaata ctgtatcagg agattttgga
      241 tgcttatcta acacacgaaa ggctcaggga agacatttgg aacaagaagg taatattaaa
      301 catgctttaa tatattacat agaatctttg ataattacta tttcaggatt agaaaacaat
      361 ttttcagcca ctgattatcc agtatattat cccgattcga tacctgacta ctcactaaaa
      421 catattcaaa cattaatgga atcattatct gatgacgatt atgattttgc ttttgatgaa
      481 gcattatttc gcttctcaat tttgaatgca aatcattttt tatctaagga agatattgac
      541 tatttaagag ttaatttacc tcgttccact gctgaagaaa taaacaatta cttaaagaaa
      601 tatgaatgtt atagtccttt aaataattta gaacttgacg attttgaata aattgactat
      661 acaaacattt atatactcga tatagtctca attttatctg atgattgccc aaatttttca
      721 ataataaaac gcataatatt atggagacaa tcccctatat tatgcgttct tttaatatca
      781 aagacttttt gacaaacttc tttgatatct aattacatgc cccctgcagg aatcgaacct
      841 gcaactactc cttaggaggg agttgttata tccattgaac taagggagct agataaaaac
      901 tctgctgaaa agcagagttt tttagtcgaa ttaacgacgg atttctttga tacgagctgc
      961 tttaccttga agagcacgca agtagtacaa tttcgcacga cgtactttac cgtaacgaac
     1021 aacttcgatt ttttcaacac gtggagtgtg gattgggaag atacgctcaa cacctacacc
     1081 gttagagatt ttacgaactg tgtagttttc tgagattcca gcacctttac gtgcgataac
     1141 aacaccttca aaaatctgga tacgttcacg gttaccttcg acaactttcg cgtgtacacg
     1201 aacagtgtca ccaggacgga atgatgggat atctgtacga agttgacctt cagtcaagct
     1261 ttggattaat ggattcattt tattctccta tctttgtcaa tcttgaggaa acttcctcag
     1321 cggataaact gtattcttgt gcgtccatta cacacaagat acagtttacc aaattttcac
     1381 aaaaaagtaa agaaatttat agcaaaattc ctaaaaaaat cgcaaccaaa ccacctatgt
     1441 aagtcaaagt caggtaagaa taaaagacct tcttatcact tagcagtctt tgaagttcgt
     1501 cattcaaggt cgaaaaagtt gttaaaccgc cacaaaatcc tgttgctaga atagcatata
     1561 cttccttaga ctctacatga ttgtagaata ctccaattaa aaaacaacct aaaagattag
     1621 ctatgagcgt tccgagtggc aatttagaag cttgattata gcgagaaaag aaataccgta
     1681 cgagtgctcc gaaaccacaa gcaattgcaa gatagacaat taccatttct tcctccctag
     1741 aaaataagct aagagtaggc ctccaccgat gctcaaaagc aaatacagga ctaaactaaa
     1801 ataacgccct gtatcaagca gcttcacagt atcaagcatt aggctggaaa aagttgttaa
     1861 acctccgcaa aaacccgtcc ccagtgctaa aatcaagccc ttactagtcc ccttatagac
     1921 cagatagccc ttgacaacaa aaatcaagca gaaaattccc agatagttga caaagagagt
     1981 gccccaagga aagtctggac tggctggtaa ccaggtggaa actagatatc ggacaagtcc
     2041 gcccaacata gcagctagaa aaattcctag cggataaaat tgttcttttt tcatttgata
     2101 ttttgatcct gataatcacg cgaacgtttg agtatgtccg aaaaagtcgc tacaatagtc
     2161 tcctgatagc gcttgtcctc tacacgactc ctaacctttt caaaaataac ttcttctctc
     2221 ttagtatcta aaatcggttt acctgaagct ttcttataag caaccacccc ctcaactaaa
     2281 tgcattcgtt cttctaacag tttgacaatt tggtcatcga tttgatcgat ttcttgacga
     2341 ataatatcta aatccataca gtctcctcct ttatttgaat tattgtatca aaaagccatc
     2401 aattaggcta gtaaaatccc aactctcgat aagaaaaatg cactgattga ttacatcagc
     2461 gcacttttat gcttatacta ctttagcaac caattcttct gcgaattgtt ccaagcgttc
     2521 aatatcttct tcctcggcag aaagatcaac tttgacacac tcagaacctt tttctgctcc
     2581 tgttgacaca aagatacggt caaagtcatc aacagcctta cagaattcat cgtagaaggt
     2641 atctcctgag ccgaccactc cgtagatttt gccattcaag ttgagatctg ctaggtcttc
     2701 gtagaagtcc atcatctcat ctggcaattc accatcacca taagtatagg tcgcaacgat
     2761 agcgatgtct gcttccaaga agtctgaagc gtcaacagtt gtacattcat caacatcaac
     2821 atccaagccc aagtcacgta atttgtctgc tacaatatct gcaatttctt cggtattacc
     2881 ggtcatactg gcaaatacaa tttttgctaa tgccatatcg tcctcctcaa tttatctttc
     2941 tccattatat cacatctttt tcattttaaa aataaaaatt cttgtgctac aatgaaatga
     3001 gaaagaattg aggttattta tggagatttg ccaacaaatt ttagaaaaaa ttaaagaata
     3061 cgacaccatt atcattcatc gtcatatgaa accagaccct gatgccttgg gaagtcaggt
     3121 gggattgaaa gccttgctgg aacatcattt cccagaaaaa accatcaaag ccgtcggttt
     3181 tgatgaacca actcttactt ggatggctga gatggatctt gttgaagata gagcctacca
     3241 aggcgcactt gtcatcgtct gtgatacagc taatactgct cgtatcgatg ataagcgcta
     3301 tagtcaaggt gattttctca ttaagattga ccaccatcca aatgatgatg tatacggtga
     3361 cctgtcttgg gtcgatacta gttcaagtag cgctagcgag atgattaccc tatttgccca
     3421 aacaacccaa ctagccttgg cagatcgcga tgctgagttg ctctttgcag gaattgtcgg
     3481 tgatacaggt cgcttcctct acccttctac cactgcacgg actcttcgcc tggctgctta
     3541 tttgagagaa cataactttg actttgcggc tctcactcgc aaaatggaca ctatgagcta
     3601 caaaattgct aaactgcaag gctacatcta cgaccatctg gaagtggatg aaaatggtgc
     3661 tgctcgcgtt atcctgagtc aagaagtctt gaaacaatac aatgtaaccg atgctgaaac
     3721 tgcagccatt gtcggtgcac ctggacgcat tgacagagtg agtctctggg gaatttttgt
     3781 cgaacaggct gatggccact accgagttcg cttacgcagt aaaatccatc ctatcaatga
     3841 aatcgccaag gagcatgatg gtggaggcca ccctctagca agtggtgcta attcctatag
     3901 cctagaagaa aacgaaatca tctaccaaaa gttaaaaaac ttgcttaaaa actgataaaa
     3961 tacttgccaa acttttcaga atctgataga ctagtatggt aacaatctat ggctcgcaaa
     4021 gagaccatgg cagaaaggaa atattgcaaa atgaaaaaag atatccatcc agaatatcgc
     4081 ccagttgtct tcatggacac aactactggt taccaattcc ttagcggttc aacaaaacgc
     4141 tctaacgaaa cagttgagtt cgaaggcgaa acttacccat tgatccgtgt ggaaatttca
     4201 tcagactcac acccattcta cactggacgt caaaagttca ctcaagcaga tggacgcgtg
     4261 gatcgtttca acaaaaaata cggtctcaaa taatgataaa agaacagttt cggctgttct
     4321 tttttgtttc ttgaaatcaa ctgctgtttt catgttccag actcatctgt aggttcggtt
     4381 tccatgctac taggcaggaa ggaaatagct gtttcaacac gtccataatg agctgtacta
     4441 ttgtcacgaa ccacactttc attgatggtc caagtggaat tcattttctt aaaagcttct
     4501 cggacttttt ccaaatcttt ggaggcaatg gcctgctcta aggtttcaaa acgaggactt
     4561 agacggttta ccaatttttc cttttccgca tctaggtcaa caggattttg ttctttttca
     4621 aaagctaata gagctgagga aatttcggtg agtttggctt cagtaatatc tccagataga
     4681 gctaactttt ccttaacaat cttaccaacc tcagaatcct tattttctac tctttcgaag
     4741 tctgatgcca tcttcgtaac gagtttctgg gtctcagtct gaccaccatt tttaaccgct
     4801 ttggatgcat ctgtgatttt cacaaagaaa aaactcaagc tttcttgggc acctgctggg
     4861 gaaagaatta aaaatgacaa agctaaaact agtaaccagc ttttagcctt ccaataattt
     4921 ctgaccaatg tagcctcctt tctccacacc accaaagcaa gtaaaaagtc cactacctat
     4981 gtgagtgatg tattcattca tcttatcagt agcacctagg ttagtttgaa ccttaacaaa
     5041 gttatcagga tccttctgga aagaaatgaa gagcaatccc gtatcaaact gaccagtctt
     5101 ttcatcaatg ccatctgagt aagagtaaga acaacgcaaa agtggcttgt ccacttactt
     5161 agccaaacga acatgcgaat catctagcaa aaaactcaaa ttcacttcat caaactcatt
     5221 tttttaccga aaggggcact actttccttg taacgaccaa aggtattttc ttgttcttcg
     5281 agaccagtgc ggtcccaggt ctctaaaaac atctgaatcc gacgaactgc catataagaa
     5341 ccattttcca tccagtcctt actatcagcc cagataacgc ggtcaaagtc ttgctccttg
     5401 gttgaatttg ctgttccatc cttaaaacca aagaggttcc acggagtctc cattcgtccc
     5461 cgatagctgc aaatccagac tgactccaac gaaggggcac cgcatttctc cccttacgta
     5521 tgagattgcg aataacgtga aaggcaattt gctcatcatc tgcacaagca tgaatcacaa
     5581 tatctccacc agtatatttt tcacgtaatt gctccttagg aaatagaggt aaatccctga
     5641 acagtcgagg gcgcttgttt tccaagttca tctttttcaa gaaacttgca gatacaccaa
     5701 aagtcagcgt caaacgatga ggattgagcc caactgtctc accagtatcg ctcggaggaa
     5761 agagagcatt ctgtccatct tttttaacca attccccctc gaccaacttg gcactataat
     5821 ccgtccaatc cttgaatagc tggataattt tatctctatc cgtcgtatgc aggtccaaga
     5881 caacaaaata gatattcttt tgcatgggcg tactatttgg tacatcaagc gaaccttacg
     5941 aaaacgtcta ctatatttaa accgctttct tgtcttcttg aattgagatt ttatttttag
     6001 acgcgacaat ggtttgagcg tctgctttct taggatgttc tggacgtggc ataatatttc
     6061 gccaaccatg gcgc
//
LOCUS       DBERDK010000095         6049 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.095, whole genome shotgun sequence.
ACCESSION   DBERDK010000095 DBERDK010000000
VERSION     DBERDK010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6049
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.095"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(89..838)
                     /locus_tag="ACL73L_001923"
     CDS             complement(89..838)
                     /locus_tag="ACL73L_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HGS0772838.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTDKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPIDRENPSASAIKYPINV
                     LKKSDRSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGPMTLKGLISRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQRLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            971..3307
                     /locus_tag="ACL73L_001924"
     CDS             971..3307
                     /locus_tag="ACL73L_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HGS0772839.1"
                     /translation="MDKNKIMGLTQREVKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNFAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DGLLLSGSFLASGSVLSQVHHVGADNYAAKLMLEAKTVKPINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALLLKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVETLARVDMLCLDKTGTITQGKMQVEAVLPLTETYGEEAIASILTSYMA
                     HSEDKNPTAQAIRQRFQGQVVYPMLSNLPFSSDRKWGAMELEGLGIVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKSSDIQALALLEILDPIREGAAETLNY
                     LRSQEVGLKIISGDNPVTVSSIAQKAGFADYHSYVDCSKITDEELIALAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICIASALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEPNFLRKSMLRALPSALMVVFSVLFVKIF
                     GSSQGWSELEISTLLYYLLGSIGFLSVFRACMPFTLWRVLLIVWSVGGFLATALFPRI
                     QKLLEIATLTGQTLPVYGVMMLVFTVIFILTSRYQARK"
     gene            3409..4086
                     /locus_tag="ACL73L_001925"
     CDS             3409..4086
                     /locus_tag="ACL73L_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HGS0772840.1"
                     /translation="MISKRLELVASFVSQGAILLDVGSDHAYLPIELVERGQIKSAIA
                     GEVVEGPYQSAVKNVEAHGLKEKIQVRLANGLAAFEETDQVSVITIAGMGGRLIARIL
                     EEGLGKLANVERLILQPNNREDDLRIWLQDHGFQIVAESILEEAGKFYEILVVEAGQM
                     KLSASDVRFGPFLSKEVSPVFVQKWQKEAEKLEFALGQIPEKNLEERQVLVDKIQAIK
                     EVLHVSK"
     gene            4073..4870
                     /locus_tag="ACL73L_001926"
     CDS             4073..4870
                     /locus_tag="ACL73L_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HGS0772841.1"
                     /translation="MLASEVIQAYEAFCPQEFSMEGDSRGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLGIEETTYLQETGPERGIGRIGNIQPQTFWELAQQVKQVFDLDS
                     LRMVHYQEDDLQKPISRVAICGGSGQSFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVLFVEKIATLLTQWKEKKGWELEILPSQASTNPFRHI"
     gene            4887..5990
                     /locus_tag="ACL73L_001927"
     CDS             4887..5990
                     /locus_tag="ACL73L_001927"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HGS0772842.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSRESDLEVTVFDHEQGQATKAAAG
                     IISPWFSKRRNKAWYKMARLGADFYVDLLADLEKSGQKIDFYQRSGVFLLKKDESKLE
                     ELYQLALQRREESPLIGQLAILDQASANELFPGLQGFDRLLYASGGARVDGHLLVTRL
                     LEASQVKLVKEKVTLTPLASGYQIGEEEFEQVILATGAWLGDMLEPLGYEVDVRPQKG
                     QLRDYQLVQDMEAYPVVMPEGEWDLIPFAGGKLSLGATHENDMGFDLTVDETLLQQME
                     EATLTHYLILAEATSKSERVGIRAYTSDFSPFFGQVPELAGVYAASGLGSSGLTTGPI
                     IGYHLAQLIQDKELTLDPLNYPIENYVKRVKSE"
BASE COUNT         1645 a         1216 c         1480 g         1708 t
ORIGIN      
        1 aaatgtttta gaagtagagg tgtactattc tagttccaat ctactatata aatgaaaacc
       61 ttagctgttt ggccaaggtt catttcttct atgcaagttc ttctcttttc ttatctgggt
      121 tccagatgaa gcttgcgata aagctaaaga tgatggttag gatagcgagg ataatagcaa
      181 ggatgagggc agggatgcgg ataaaccacc agagtggatt tggtttttta tcattgtgcc
      241 attgtttcgt ttcttcgtcc agacgttgga attctgtttg aatacgattg gcgactgttt
      301 caatgccttc atcattcatt ttcttgatat ctgagatatc gattggattt ccaaagttca
      361 tatcgacacg ttcacggcta atcaagccct tcaaagtcat gggaccggtg taggtaaccg
      421 gcatgatacg gaccttggcc attttggcaa tcagtgctgc gccccccttg acatcgtttg
      481 agtggcggct accacttgga aacatgatga gagagcggtc actttttttg agaacgttga
      541 taggatattt gatggctgag gcgctgggat tttcacggtc gatgggaaag gcgccacaca
      601 tacgaatcca ccaaccaaag atacggttgg taaagagttc tttttttgcc ataaagatga
      661 actgttttgg cttggtcgca aaggccatat aaacaggatc ccaccaggta cggtgaggcg
      721 caactaaaat ataattttca tcttgattag gaattttatc agtattatga tagtgagcat
      781 tgccattgat ggaccataag agcaatacaa ctaatccacg caaataagta taaaacatgc
      841 gatctccttc gattgttttc ttgttattat tataccttat caaaggaggg ctggcaaact
      901 tttcccttga ctagatacat atttaggatg aaattagaat tctgttaaaa aaaatgatat
      961 aatagaattt atggataaaa ataagattat gggattaacc caaagagaag tcaaggaaag
     1021 acaggctgag ggtttggtca atgactttac cgcatcagcc agtaccagca cttggcaaat
     1081 cgttaaacga aatgtcttta ccctttttaa cgctttgaac tttgccattg ctttggctct
     1141 tgcctttgtg caggcttgga gcaatctggt cttctttgct gttatctgct ttaacgcttt
     1201 ttctgggatt gtgaccgagc tacgagccaa acacatggtg gacaagctca atctcatgac
     1261 caaggaaaag gtcaaaacca tccgtgatgg tcaggaagtt gctcttaatc ctgaagaatt
     1321 agtgctagga gatgtcattc gtttgtctgc aggagagcag attcctagtg atgccttggt
     1381 tttggaaggc tttgcggaag tcaatgaagc catgttaacg ggagaaagtg atttggtgca
     1441 aaaggaagtt gacggcttac ttttgtcagg aagtttccta gccagtgggt cagttttatc
     1501 tcaagttcac catgtcggag cagacaacta tgctgccaaa ctcatgcttg aggctaagac
     1561 cgttaaaccc atcaactccc gtatcatgaa atcgctggac aagttggctg gttttactgg
     1621 gaagattatc attccctttg gtctggctct cttgctggaa gccttgcttt taaaaggcct
     1681 gcctctcaag tcatccgttg taaactcgtc gacagctctt ttgggaatgt tgcctaaggg
     1741 aattgccctt ttgaccatta cttcgctttt gactgcagtg attaagctag gtttgaaaaa
     1801 ggtcttggtg caggagatgt actctgttga gaccttggcg cgcgtggata tgctctgtct
     1861 ggacaagacg ggcaccatca cccaaggaaa gatgcaggtg gaggctgttc ttccactgac
     1921 ggaaacttac ggtgaagagg ctattgccag catcttgact agctacatgg cccatagtga
     1981 ggataaaaat ccaacagctc aagccattcg ccagagattc caaggtcaag tagtctatcc
     2041 tatgctctct aatcttccct tctccagcga ccgcaagtgg ggagccatgg agttagaagg
     2101 tttgggaata gttttcttag gggcacctga gatgttgctg gattctgagg tcccagaagc
     2161 cagagaggcc ttggagagag gttcacgtgt cttggtctta gctctcagtc aggagaaatt
     2221 agaccatcac aaaccacaga aatcatctga tattcaggct ctagccttgc tggaaatctt
     2281 ggatcctatt cgagagggag cggcagagac gctgaactat ctccgttctc aggaagtggg
     2341 gctcaagatt atctctggtg acaatccagt tacggtatcc agcattgccc agaaggctgg
     2401 ttttgcggac tatcacagct atgtcgattg ttcaaaaatc acggatgagg aattgattgc
     2461 tctggcggag gagacagcga ttttcggacg tgtttcccct catcaaaaga aactcattat
     2521 ccaaacgctg aaaaaagcgg gtcatacaac ggctatgaca ggggacgggg ttaatgatat
     2581 cttggccctt cgtgaggcgg attgttctat cgtaatggcg gagggagacc cggcaacccg
     2641 tcagattgcc aacctggttc tcttgaactc agactttaat gatgttcctg agattctttt
     2701 tgaaggtcgt cgtgtggtca ataacattgc ccacatcgct ccgattttct tgataaagac
     2761 catctactca ttcttgctcg cagttatctg tatcgccagt gctttactag gtcggtctga
     2821 atggatcttg attttcccct tcattccaat ccagattacc atgattgacc agtttgtgga
     2881 aggtttccca ccattcgttc tgacttttga gcgaaatatc aaacctgttg agccaaattt
     2941 cctcagaaaa tccatgcttc gtgccctacc aagcgctctc atggtcgtgt ttagcgtcct
     3001 gtttgtgaaa atatttggga gtagccaagg ttggtctgag ttagaaatct caactctcct
     3061 ctattatctc ttggggtcaa ttggtttctt atccgtattt agagcctgca tgccatttac
     3121 cctatggcga gtcctattga ttgtctggtc agtaggaggc ttcctagcca cagctctctt
     3181 cccaagaatt caaaaactgc ttgaaattgc aaccttaaca ggacaaacat tacctgttta
     3241 tggtgtcatg atgttggtct ttaccgtgat tttcatcctg accagtcgct atcaagccag
     3301 aaaataaaga aaggctgcaa tctgtggatt gcagtttttt taggtacaag attgccagtc
     3361 gaaatgtggt ataataagag gtaatagagt tttggaaagt gagagaagat gatttcaaag
     3421 agattagaat tggtagcttc ctttgtgtca cagggggcta ttttactaga tgtgggaagt
     3481 gaccatgctt atctgcctat cgagttggtt gagagaggcc aaatcaaaag cgctattgca
     3541 ggtgaggtgg tggaaggtcc ctatcagtct gcggttaaaa atgttgaggc tcacggccta
     3601 aaggagaaaa tccaagtccg tttagccaat ggcttggcag cttttgaaga gactgaccaa
     3661 gtgtctgtca ttaccattgc tggcatgggt ggtcgtttga ttgctaggat tttagaagaa
     3721 ggtttgggga agttagctaa tgtagagcgt ttgatcctcc agcccaataa tcgtgaagac
     3781 gacttgcgta tctggctaca ggatcatgga ttccagattg tagcagaaag catcttagaa
     3841 gaagctggaa agttttatga gattttggtg gtggaagcag gacaaatgaa gctatcagcc
     3901 agtgatgttc gctttggtcc cttcttgtcc aaagaagtca gtccagtatt tgtccaaaaa
     3961 tggcaaaaag aagctgagaa gctagagttc gccctcggac aaatcccaga aaaaaatctg
     4021 gaagaacgtc aagttctagt agataagatt caagctatca aggaggtgct ccatgttagc
     4081 aagtgaagtg atccaagctt atgaagcctt ttgccctcag gaattttcta tggagggaga
     4141 cagccgtggt ctgcaaattg gcactttaga caagggtatc caaagggtca tggttgccct
     4201 cgatattcgt gaagagacag tggcagaggc cattgaaaag ggtgtggact tgattatcgt
     4261 caagcacgcg cctatctttc gtcctatcaa ggacttgctt gccagccgtc cgcaaaatca
     4321 gatttacatc gacctgatta agcatgatat cgcagtttat gtcagccata ccaatattga
     4381 tatcgttgaa aatgggctca atgactggtt ttgtcagatg ctaggaatcg aggagacgac
     4441 ttatctgcag gaaacaggtc cagaacgtgg aattggacgt attgggaata ttcagcctca
     4501 gacattttgg gaattggccc aacaggtcaa gcaagtcttt gacctagata gccttcgaat
     4561 ggtgcattat caagaggatg atttgcagaa gcctatttca agagtagcaa tttgtggtgg
     4621 aagcggacag tctttctata aggatgcttt ggcaaagggg gcagatgtct atatcactgg
     4681 tgatatctac taccatactg ctcaggatat gctgtctgat ggcttgttag cattggatcc
     4741 aggtcactat atcgaagtgc tttttgtgga aaaaatcgca acactcctta ctcaatggaa
     4801 agagaaaaaa ggctgggaac tagagatttt acctagtcaa gcatcgacca atcctttccg
     4861 tcatatctag ttagaaagtg aagacaatga aaaaagttgc cattattgga gcaggaattg
     4921 tgggagcaac agctgcctac tacctctcga gagaaagtga cctagaggtg accgtttttg
     4981 accatgagca aggtcaagcc accaaggctg cagcaggaat tatcagtcct tggttttcca
     5041 aacgccgtaa taaagcctgg tacaagatgg cgcgcttggg ggctgatttt tatgtggatt
     5101 tattagctga tttagagaaa tcaggacaaa aaatcgactt ttaccagcgt tcgggagtct
     5161 ttctcttgaa aaaggatgaa tccaagttgg aagaacttta tcaactggcc ctccagcgca
     5221 gagaagaatc tcccttgata gggcaattag ccattctgga ccaagcctca gctaatgaat
     5281 tattccctgg tttgcaggga tttgaccgcc tgctctatgc ttctggtgga gcgagagtag
     5341 atggccacct cttagtgact cgtttgctgg aagccagtca ggtcaagctg gtcaaagaaa
     5401 aagtgactct gacaccgtta gcatcaggct accagattgg tgaagaggag tttgagcagg
     5461 ttattttggc gacgggagct tggttggggg acatgttaga acccttgggt tatgaagtag
     5521 atgttcgtcc tcaaaaagga caactacgag attatcagct ggtccaagac atggaagctt
     5581 accctgttgt catgccagaa ggggagtggg atttgattcc ttttgcaggt gggaaattgt
     5641 ccttaggcgc tacccacgaa aatgacatgg gatttgattt gacggtagat gaaaccttgc
     5701 tccaacaaat ggaggaggcc accttgactc actatctgat tttggctgaa gctacttcaa
     5761 aatctgagcg tgtgggaatc cgtgcctata ccagtgattt ctctcccttc tttgggcagg
     5821 tgccagaatt ggcaggtgtc tatgcagcca gtggactagg ttcatcaggc ctcacaactg
     5881 gtcctatcat tggttaccat ctagcccaac tgatccaaga caaggagttg accttggacc
     5941 ctctaaatta cccaattgaa aactatgtca aacgagtaaa aagcgaataa tactcaatga
     6001 aaatcaaaga acaaactagg taactagccg cgggttactc aaagcactg
//
LOCUS       DBERDK010000096         5781 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.096, whole genome shotgun sequence.
ACCESSION   DBERDK010000096 DBERDK010000000
VERSION     DBERDK010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5781
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.096"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            17..970
                     /locus_tag="ACL73L_001928"
     CDS             17..970
                     /locus_tag="ACL73L_001928"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HGS0772843.1"
                     /translation="MKNKRIFKDFQASKMSLNIYTSPLLAFVFVFIGEFVAFTLYGIG
                     LLALIGLARNFGEAGQNLATYLQTLHQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENFLSNLLKGFSLGLALFLLTLLGLVVLGQYRLESIHLNPYSLAF
                     VVFTIPFWILQGTAEEVVARAWLLPQLASRTNLKLAILISSLFFTLLHMGNSGLTPLS
                     LVNLFLFGVAMALYLLKTDTVWGVAGIHGAWNFAQGNLFGILVSGQPSGTSLMTFLPQ
                     GNQDWLSGGSFGIEGSIMTSLVLLLLIVYLANKLKKENERM"
     gene            1047..3035
                     /gene="uvrB"
                     /locus_tag="ACL73L_001929"
     CDS             1047..3035
                     /gene="uvrB"
                     /locus_tag="ACL73L_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HGS0772844.1"
                     /translation="MINHITDNQFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREVEALTGQVLGEVDHLAIFPATHFVTNDDHMEVAVAKIQAELEEQL
                     AVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PDDFLIMIDESHMTMGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARVEKN
                     ERTFITTLTKKMAEDLTDYFKEMGIKVKYMHSDIKTLERTEIIRDLRLGVFDVLVGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADTVTQSMQ
                     RAIDETARRRKIQMAYNEEHGIVPQTIKKEIRDLIAVTKAVAKEEDKEVDINSLNKQE
                     RKELVKKLEKQMQEAVEVLDFELAAQIRDMMLEVKALD"
     gene            3045..3608
                     /locus_tag="ACL73L_001930"
     CDS             3045..3608
                     /locus_tag="ACL73L_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HGS0772845.1"
                     /translation="MIYLRKLKKEDLMSLWEMAYSQLNPVWKQYDAPYYDDYQYFSNF
                     KEFELQKSESILSNSNRLGIFVDDKLVGTVSRYWVCKETRWMELGIGIYDKKFWNTGI
                     GKVAMLQWIDRTFQDYLELEHLGLTTWSGNLGMMKLAEKLRMKKEAHIPKVRYYQGKY
                     FDSIKYGILREDWEKINDGYYQINGNS"
     gene            3574..4062
                     /locus_tag="ACL73L_001931"
     CDS             3574..4062
                     /locus_tag="ACL73L_001931"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS0772846.1"
                     /translation="MTVIIKSMETPEEIEGKSLVHWQTWREAYDDLLPAEFQETMTLE
                     RCRFFSQKYPENTLIAIGDLKVVGFISYGNFRDETIQAGEIIALYVLKDYYGKGIAQK
                     LMKAALTALNHFSEIFLWVLKDNKRAIAFYQKMGFTFDGQEKILELGKPIKEKRMVFY
                     SK"
     gene            4136..4603
                     /locus_tag="ACL73L_001932"
     CDS             4136..4603
                     /locus_tag="ACL73L_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HGS0772847.1"
                     /translation="MFRSQLTILTNICLIEDLETQRVVMQYRAPENNRWSGYAFPGGH
                     VENDEAFAESVIREIYEETGLTIQNPQLVGIKNWPLDTGGRYIVICYKATEFSGTLQS
                     SEEGEVSWVQKDQIPNLNLAYDMLPLMEMMEAPDKSEFFYPRRTEDDWEKKIF"
     gene            complement(4600..5115)
                     /locus_tag="ACL73L_001933"
     CDS             complement(4600..5115)
                     /locus_tag="ACL73L_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HGS0772848.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQSLAEHFHVTRQVIVQDIAILR
                     ADGAPILSTNRGYIYKQIDTNPYVHKLFKVKHEVEEIGQELLAIVDNGGHVQNTLIDH
                     PVYGEIETLLKLSCRRDVQHFLEQVEHSDFRPLSELTDGIHYHLVEAETQQDLHYIEE
                     ALDQLGYLVKD"
     gene            complement(5127..5654)
                     /locus_tag="ACL73L_001934"
     CDS             complement(5127..5654)
                     /locus_tag="ACL73L_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HGS0772849.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAVFVILASSFGFLMAGYPMVIVFRAFSHISFGALGALYLQKFPDTL
                     DKPKSSWVFNFVLAVVHALAEVLACVLFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
BASE COUNT         1744 a         1025 c         1304 g         1708 t
ORIGIN      
        1 aaaagaggac ggggatatga agaacaaaag aatatttaaa gacttccaag cttcaaaaat
       61 gagtttaaac atttacacaa gccccttgtt agcctttgtt tttgtcttca taggagagtt
      121 tgtggctttt actttgtatg gtattggctt gttagctctc atcggacttg ctagaaattt
      181 tggagaggct ggtcaaaatc ttgcaaccta cttgcagacc ttgcatcaga gcttgacgga
      241 taaaacaagt gactttcgtt taattttagg attactggcc tttggtttta ttcttaacac
      301 tgtgttcaga tggacaagaa aagttgagaa aagacctatt cgaaccttgg gattttatag
      361 agagaatttc ctcagcaatc ttctgaaagg atttagtcta ggcttggcac tttttcttct
      421 gaccttgtta ggtttagtgg tcttaggtca atatcgtttg gaatccattc acttgaatcc
      481 ttattctctt gcctttgtcg tctttactat cccattttgg attttacagg ggacagcaga
      541 agaagtggtg gcccgtgctt ggctacttcc tcaattggcc tcaagaacca atctaaaact
      601 agctattctt atatctagcc tgttctttac cctgcttcat atgggcaatt ctggcctcac
      661 ccctctatct ctagtaaatc tctttttatt cggagttgcc atggctcttt atcttctcaa
      721 aactgataca gtttggggtg ttgcaggtat tcatggtgct tggaattttg ctcagggtaa
      781 tctctttggg attttagtta gtggtcaacc gtcaggaacg tctctgatga cctttttacc
      841 acaaggcaat caagattggc tatcaggtgg ttcttttggc atagaaggtt ccattatgac
      901 aagtctggta ttactactgc tgattgtcta tcttgctaat aaattaaaga aagaaaatga
      961 aaggatgtga cttcggtccg tccttttctt cgtgaaaata ctataagtat gctaaaatag
     1021 gaatagcaca tggagagagg attcttatga tcaatcacat tacagataat caatttaaac
     1081 tagtatcaaa atatcaacca tcaggagatc aaccccaagc tatcgagcag ttggtggata
     1141 acattgaggg gggagaaaaa gctcagattc tgatgggggc gactggaaca gggaagacct
     1201 atactatgag tcaggtcatt tctaaagtca ataaaccaac tctggttatt gcccacaata
     1261 aaactctggc tggtcagctc tatggggagt ttaaggaatt tttccctgaa aatgcagttg
     1321 agtatttcgt atcctactat gattattacc agccagaggc ctatgtccct tctagcgata
     1381 cctatattga gaaggatagt tctgtcaatg acgagattga caagcttcgc cactcagcta
     1441 cctcagccct tttggagcgt aatgatgtta ttgtcgtggc ctcagtctct tgtatctatg
     1501 gtttgggttc gcccaaggaa tacgctgata gtgtcgttag tctccgtcct ggtctagaga
     1561 tttctcgtga taaactcttg aatgacttgg tcgatattca gtttgaacgt aatgatattg
     1621 atttccaacg cggaagattt cgcgttcgtg gggatgtggt agagattttc ccagcttccc
     1681 gagatgaaca tgcctttcga gtagaatttt ttggagacga aattgaccgt atccgtgagg
     1741 ttgaggctct gacaggtcag gtattgggag aggtggatca tttggcgatt ttcccagcta
     1801 ctcactttgt gaccaatgac gaccatatgg aagttgccgt tgctaagatt caagcagaat
     1861 tagaagagca gttagctgtc tttgagaagg aaggtaaatt gcttgaagcc cagcgcttga
     1921 aacaacggac agagtacgat atcgaaatgc tgcgcgagat gggctataca aatggggttg
     1981 aaaattattc tcgccacatg gatggacgta gtgaaggtga gcctccttat acgcttctcg
     2041 actttttccc agatgatttt ttgattatga ttgacgagag tcatatgact atgggacaaa
     2101 taaaaggcat gtacaatgga gaccgttcgc gtaaggaaat gctagtaaat tatggtttcc
     2161 gtttaccgtc tgccttggac aatcgtcctc tccgtcgtga ggagtttgaa agccatgtgc
     2221 atcagattgt ttacgtatca gcgacacctg gtgactatga aaatgaacag actgagacgg
     2281 tgattgagca aatcattcga ccgacagggc ttttggatcc tgaggtggaa gtccgtccga
     2341 ctatgggaca gattgatgat ctcttaggtg aaatcaatgc ccgtgttgaa aagaatgaac
     2401 gaacctttat caccaccttg accaagaaaa tggcagagga cttgaccgac tatttcaagg
     2461 aaatgggcat caaggtcaag tacatgcact cggatattaa gaccttggaa cggacggaga
     2521 ttatccgtga tctccgcttg ggtgtctttg atgtcttggt tggaatcaat ctccttcgtg
     2581 aagggattga cgttcctgag gttagcttgg ttgccattct cgatgctgac aaggaaggtt
     2641 tccttcgtaa cgagcgtgga ctcatccaga ccattgggcg tgccgcccgt aacagtgaag
     2701 gtcatgtcat catgtatgca gatacggtta cccagtctat gcaacgtgct atcgatgaaa
     2761 ctgcccgccg tcggaaaatt cagatggcct ataatgaaga gcatggtatc gtaccacaga
     2821 caatcaagaa agaaatccgt gacctgattg ccgtgaccaa ggcagttgct aaggaagagg
     2881 acaaggaagt cgacatcaat agcctcaaca aacaagagcg caaagaactc gtcaagaaac
     2941 tagaaaaaca aatgcaagaa gccgtcgaag tgcttgactt tgaactggcg gctcagatcc
     3001 gtgatatgat gttggaagtc aaggccttgg attaggggaa tagtatgatt tatttaagaa
     3061 agttaaagaa agaagatttg atgtctttat gggaaatggc ttattcacag cttaatccag
     3121 tttggaaaca gtatgatgct ccctattatg atgattatca gtatttttca aattttaaag
     3181 aattcgaact acaaaaatca gaatccattt taagcaactc aaatcgcctt ggtatttttg
     3241 ttgatgataa actagttggg actgtttcgc gttattgggt atgtaaagaa acaagatgga
     3301 tggaattggg aattggtatt tatgataaaa aattctggaa cactggtatt gggaaagttg
     3361 ctatgttgca gtggatagat aggacgtttc aggattactt ggagttggag catctgggtt
     3421 tgacaacttg gtcaggaaat cttggtatga tgaaacttgc tgaaaaatta agaatgaaaa
     3481 aagaagctca tattccaaaa gttcgttatt atcaaggtaa atattttgat agtattaaat
     3541 atggtatttt gagagaagac tgggagaaaa taaatgacgg ttattatcaa atcaatggaa
     3601 actcctgaag agatagaagg taaatccctc gttcactggc aaacgtggag agaggcttat
     3661 gatgaccttt tgcctgcaga atttcaggag acaatgacat tagaaagatg tcgattcttt
     3721 agtcaaaagt atccagaaaa tacattgatt gcgataggtg atctgaaggt agttggtttt
     3781 atcagttatg gtaactttcg tgatgagact attcaagctg gtgaaattat tgctttatat
     3841 gttttaaaag actattatgg aaaaggtatc gcacaaaagt taatgaaagc agctttgact
     3901 gctcttaatc atttttctga aattttctta tgggtattga aagataacaa gcgcgccatt
     3961 gctttctatc aaaaaatggg ttttactttt gatggacaag aaaaaatact tgaacttgga
     4021 aagcctataa aggaaaaacg aatggtgttc tattctaaat aattttaaaa aagtaaaaga
     4081 taatatctct ggtagtaagt ctgaaaattt aataaattag aaagcgagta aatttatgtt
     4141 ccgttcccaa ttaacaattt taacaaatat ctgtctgatt gaagacctcg aaactcagcg
     4201 cgtggtgatg cagtatcgcg cccctgaaaa caatcgctgg tctggttatg cctttcctgg
     4261 aggtcatgta gaaaatgatg aggcttttgc ggagtctgtc attcgtgaaa tctacgaaga
     4321 aacagggttg actatccaaa atcctcaact tgtcggcatt aaaaattggc cactagatac
     4381 aggtgggcgc tatattgtca tttgttataa ggcgactgag ttctctggta cccttcaatc
     4441 ttcagaagag ggagaagttt cttgggtgca aaaagaccag attccaaact taaatctggc
     4501 ctatgatatg ctaccattga tggaaatgat ggaagctccc gacaagtcag agtttttcta
     4561 tcctcgccgt acagaagacg attgggaaaa gaaaatcttc tagtctttta ctaaataacc
     4621 tagctgatcc aaggcctcct cgatatagtg gaggtcttgt tgtgtttcgg cttcgactag
     4681 gtggtaatgg atgccatctg tcaattcaga tagaggtcta aagtcagaat gctcgacttg
     4741 ttctagaaaa tgttgcacat ctctgcgaca agacagttta agcaaggttt caatttctcc
     4801 ataaacggga tggtcaatca aggtattctg gacatgccca ccattatcga caatagcaag
     4861 gagttcctga ccgatttctt caacttcatg tttcactttg aaaagtttgt gaacataagg
     4921 attggtatca atttgcttat agatgtagcc acgattggtg gataggatag gagcgccatc
     4981 ggctctcaaa attgcaatgt cctgcacaat gacctgacgt gtgacatgaa aatgttccgc
     5041 caaactttgg ccattaaggg ctttaggagc ttctttcaag agttggagca gggcttgttt
     5101 gcgatctttt gtcatagctt ttccttttaa cggcgttttc gaagcacttt atagacagct
     5161 agtgctaatg tatagtctac catactatgg ataattgtgc caaatccaac tagtacaaat
     5221 agaacataga acatgttttc tacattggta cccgaagttg cgtaaaaaag gacacaggct
     5281 aatacttcag caagggcatg aacaacagcc aaaacaaaat tgaaaaccca ggaagatttt
     5341 ggtttatcta gggtatcggg gaatttttgt aggtaaagag ctcctaaagc accaaaagat
     5401 atatgggaaa aagcccgaaa aacgataacc atgggatagc cagccatcaa aaatccaaaa
     5461 ctagaggcta ggatgacaaa aactgccatc aagggcgaca agaacatggc tataaaaata
     5521 gcgatgtggc tccccaaagt ataggaagca ggtggaatga caatcttgaa aggcataaca
     5581 attggaatca aaatcgcaat agccgttaaa agggctgtca ttgtcataaa ttgtgtcttt
     5641 ttccgtgtat tcacaagaat ctccttttta actgcatata cactagtatg gtacaataaa
     5701 ccagacaata aagcaagaat ttacttgggt ttatagatca ttttttagtt aaaagttata
     5761 gtagattgaa actagaatag t
//
LOCUS       DBERDK010000097         5464 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.097, whole genome shotgun sequence.
ACCESSION   DBERDK010000097 DBERDK010000000
VERSION     DBERDK010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5464
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.097"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..407
                     /locus_tag="ACL73L_001935"
     CDS             <1..407
                     /locus_tag="ACL73L_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGS0772850.1"
                     /translation="PESDNKNLDDIIKLITGCPIKFNVSGNKGQKNNIELIAKQRSFI
                     DYWAKYVSYLPFPTPEFFLANLCNSVDREGYDFSKDGNGKEYFRKKTQVALGIENITS
                     EDIFQEQRRAVSKIQPESSMFQCIKEKLEALF"
     gene            428..569
                     /locus_tag="ACL73L_001936"
                     /pseudo
     CDS             428..569
                     /locus_tag="ACL73L_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155197.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            787..1590
                     /locus_tag="ACL73L_001937"
     CDS             787..1590
                     /locus_tag="ACL73L_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HGS0772851.1"
                     /translation="MQSTYNIDNPNLSYEAKRDLWRIGFGLQKVDNLVPSAYMESLAE
                     KQSRGELTYEQVYEDATVYHHTVDASTEEADLVSLRIVELLSRRGFRFSPATLLAIHK
                     ELFQDIFEPSIPVGQFRQTNITKNEPVLNGESVVYSDYSMIQMTLDYDFNQEKQVAYA
                     TLTQADVVKQIQHFISGIWQIHPFREGNTRTVTVFLIQYLREFGFDIDNTPFQQHSKY
                     FRDALVLDNAKILQRRSEFLTAFFENLLLGGQNDLSSEKMYLDLDLDFS"
     gene            1862..3232
                     /locus_tag="ACL73L_001938"
                     /pseudo
     CDS             1862..3232
                     /locus_tag="ACL73L_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397831.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
     gene            3244..4644
                     /locus_tag="ACL73L_001939"
     CDS             3244..4644
                     /locus_tag="ACL73L_001939"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HGS0772852.1"
                     /translation="MSERKTKTIILVHNKQLLDQWLDRLNCFLTFEEEEAIRYTASGR
                     EKVIGYVGQYGGTKKWLSKLVDVVMIQSLFKLENSQSLLDEYEMMIVDECHHVSALMF
                     EKVVAQFRGKYLYGLTATPERKNGHEPIVFQRIGEILHTADKRETDFKRQLQLRFTSF
                     AHLEIEKTKASNFIQLSDWIATDSARNQLILKDILAQVAEGRNILVLVNRIQQIDVFE
                     KLLKEKEVDGCYIISGKTKVRERTSLLEILEQLDKGFVLLSTGKYIGEGFDLPQLDTL
                     ILAAPFSWKNNLIQYAGRIHRNYKDKSLVRIFDYVDIHVPYLEKMFQKRQVAYRKMDY
                     RAIEGEEKQFVYVDSRYENVLRKDLAGERQECLLVLPYVHQTKLMNFLKEFRISQIEI
                     CIPETVANKAWLDQLKSKKIKVSFTQSKIVTPILLVNKTIVWYGAMPLLGKVDEMTIL
                     RLESASIVSELVAGLR"
     gene            4944..>5464
                     /locus_tag="ACL73L_001940"
     CDS             4944..>5464
                     /locus_tag="ACL73L_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HGS0772853.1"
                     /translation="MIIKRILNHNAVIAQSKKDIDILLFGRGIAFGRKTGDKVNPIDI
                     EKSFFLKNRDNMTRFTEMFINVPLELVYITEKIINLGKITLGNNFDEIIYINLTDHIS
                     SSIERYKEGIIISNPLRWEISKYYKEEFELGKRALQIIKKELGIELPIDEAAFIALHF
                     VNANLENNFQESY"
BASE COUNT         1754 a          806 c         1130 g         1774 t
ORIGIN      
        1 ttcctgaatc agataataaa aatcttgatg atattataaa attgataacg ggatgtccaa
       61 ttaaatttaa tgtttcaggt aataaagggc aaaaaaataa tattgaatta attgcgaaac
      121 aaagaagctt tatagattat tgggctaaat acgtttctta tcttcctttc ccaaccccag
      181 agtttttttt agctaatttg tgtaacagcg tagataggga aggttatgat ttttctaaag
      241 atggcaacgg aaaagaatat tttagaaaaa aaactcaagt agcgttagga atagagaata
      301 taacttctga agatattttc caggagcaaa gaagagctgt ttcaaaaata cagccagaat
      361 catcaatgtt tcagtgtatt aaagaaaaat tagaggcatt gttctaattc cttagattag
      421 gtactacata acaaattttt taaattcaaa cccgcaccaa aaacaacatt gcaactttct
      481 tctcaggtaa tgtgttggga tttgaacaga ccaacgagtt catagtggtg tatgccaatg
      541 actttgataa gtctacgcgt cagacttatt agttcattta tccagatata tctcgataat
      601 catattattt agtcaaagaa catcccatat gagggcatga aatgctaagt ttagcttgct
      661 cactattaag atgaagtata tatatttcat agaacctatt gtgaaggatt tttataattt
      721 tcgccaattc catacctatt tcacaattta ccgatgacac agaaaaacaa ttgaaaggat
      781 atttctatgc aatcaactta caacattgac aatccaaact tgtcttatga agcgaagcgt
      841 gatttatggc ggataggttt tggtctgcag aaagttgaca atctagtgcc atcagcgtat
      901 atggaatctt tggctgagaa acagtcccgg ggtgaactga cttatgagca ggtttatgag
      961 gatgcaacgg tttatcatca taccgttgat gcgagtacgg aggaggcaga cttggtttct
     1021 ttgcgtatcg tagaactctt gtctcgaaga ggttttagat tcagtcctgc gactttactt
     1081 gctattcata aggagttgtt tcaagatata tttgaaccct ctattccggt ggggcaattt
     1141 cgtcagacca atatcacaaa gaatgaacct gttttgaatg gtgaaagtgt tgtgtactct
     1201 gattactcca tgattcaaat gaccttagat tatgatttta atcaggaaaa acaagttgca
     1261 tatgcgacac taactcaggc ggatgtggtt aaacaaatcc agcattttat ttcaggaatc
     1321 tggcagattc atccatttcg cgaaggaaac actcggacag taacggtctt tttgattcag
     1381 tatcttcgtg agtttggttt tgatattgat aatacaccat ttcagcaaca ttccaaatat
     1441 tttcgtgatg ccttggtatt agataatgca aagattttac agcgacgttc tgagttttta
     1501 acagcttttt ttgaaaatct cttgctcggt ggtcaaaatg atttgtcttc agaaaaaatg
     1561 tatctagatt tagacctcga tttttcataa tccaaatact gagtaaacat tgaattttag
     1621 gcaaaaatga agtaaatatt ctcacaagaa aacgtatatc atcaaagttt taggtcgctt
     1681 gataatatgc gttttttgaa ttttatagac tgctcgttta aactctattt acctcgtaca
     1741 ttccggagcg agatggacta ttggtcgcat acaaaatgag tgttattcgg attttatttt
     1801 cgtgtacaat ttcagccatt cttgttataa tcagcctata ggaatcaagg aggtgattgt
     1861 tatggctgtt tttgtgtctt tggatggaat tgtggtagaa gtacttgatg tcttttcttc
     1921 ttttaatggg gatagtgagt ttttcttgtg taaacgtctt aaagacaaga gtcagtttgt
     1981 gatggaacgc tctcggttcg aggagatgtt ccaacttcaa agtagtcact tgacgacgca
     2041 agaaaaatta caattgttta cctccgtgtt tgctggacgt tatgatgttt atgctaagaa
     2101 ttttatcaat gaacaaggga aaattcagta ttttccttcc tatgattatg gttggaagca
     2161 gttgccacct gaaaaacgga gtttccagac attgacgaac tccgttttga aatctcattt
     2221 tcgtggggag gcagctatcg gtatctttcc tatgcactta gatgatagct gttatttttt
     2281 ggtactggat ttggatgaag gagattggaa agaagctggt ttaaccattc gaagaatagc
     2341 cagggaacgc cagatggaag cccatttaga gatttctcgt tcgggttacg gactccatat
     2401 ttggttcttc tttgaggaag cgattctgag tcgaaaggct cgcctgtttg gaaagaaact
     2461 attagaattg gctatgcagg aaagtatgca actgtccttt gattcttttg atcgcatgtt
     2521 tccaaatcag gatgttcttc ctaaaggtgg atttgataat ttaatttccc ttccttttca
     2581 aggagaagct tatcatcaag ggcgaacggt ctttgtggat gaacagtttc agccttatga
     2641 agatcaatgg aggtatctac aagaaattca gaggatttca actgctaaag tggcactgtt
     2701 aatccaagag gagttaggca agcaagaatt ggataaggag ttgaaggtcg ttttatccaa
     2761 tatgatccaa cttgaaaaat cgtctgtgac atccaaggca ctttttttct tgaaaaatat
     2821 ggcttccttt tctaatcccg aattttatag tagattgaaa ctagaatagt acacctctgc
     2881 ttctaaaaca ttgttagaaa tcgatttgac tttcctgatc gatttgtcct gttattattt
     2941 cattttacta tatttaaagc aggctatgcg acagccaatc tatcaaattc ctgagcgaat
     3001 gtatttattt ggagaatccg attattattt atggctgcca agaggcttgt tagatccatt
     3061 gcaagataaa tttaagcagg tagttgtgga ggataggaga aaagtacaaa ggtctattag
     3121 agtagcattt aagggagagt ttactttgga gcaagaatta gccctgtcag atatgaattc
     3181 taaagaaaat ggtttacttc atgcgggaca ggttttggga agaccgtttt aggtgctgct
     3241 cttatctctg aacggaaaac aaaaacaatt attctagtcc ataataagca actcttagac
     3301 caatggctag atcgcttaaa ctgctttttg actttcgaag aggaggaggc tatccgttat
     3361 acggcatcag gtcgtgaaaa ggtaatcggc tatgttggac agtacggtgg gactaagaaa
     3421 tggctgagta aactggttga tgtcgttatg attcaatctc tatttaagtt ggaaaatagt
     3481 caaagtcttt tggatgagta tgagatgatg attgtggatg agtgtcatca tgtctctgcc
     3541 ttgatgtttg aaaaagttgt tgctcagttt agagggaagt atctttacgg tttgacggct
     3601 acgcctgagc gtaagaatgg tcatgagcct attgtttttc agagaattgg tgagatactc
     3661 catactgctg ataagaggga aacggatttt aaacggcaat tgcaattaag attcacttct
     3721 tttgctcatt tggaaattga aaagaccaaa gcaagtaatt ttatacagct cagtgattgg
     3781 attgctacag actcagcgag gaatcagttg attctcaagg atattctagc ccaagtggca
     3841 gaaggtcgga atatcttggt tctagttaat cgaattcaac agatagatgt ttttgaaaag
     3901 ttattgaaag agaaagaggt tgatggctgt tacattatta gcggaaaaac caaagtccga
     3961 gagagaacaa gtttactgga gatattagaa cagctagata aagggtttgt attgttgtca
     4021 actggaaaat acattggcga aggttttgac ttacctcagt tggacacgct gatcttggca
     4081 gcgccctttt cttggaaaaa taatttgatt cagtacgccg gtcggattca tagaaactac
     4141 aaagataagt ctttggtacg tattttcgat tatgtggata ttcatgttcc ttatttagaa
     4201 aagatgtttc agaaacgaca agtagcttat cgaaagatgg attatcgtgc cattgagggt
     4261 gaggagaaac aattcgttta tgttgatagt agatatgaga atgtgttgag gaaggactta
     4321 gcaggggaaa gacaggaatg tctgcttgtt ttaccttatg tgcaccagac aaaactgatg
     4381 aattttctaa aagaatttag gattagtcaa attgagatat gtataccaga gacggttgca
     4441 aataaagcat ggctagacca gttgaagagc aagaaaatta aagtgtcttt tactcaatca
     4501 aaaatagtaa cgcctattct tttggtgaat aagactattg tttggtatgg tgcaatgcca
     4561 ttattaggga aggtagatga gatgaccata ttacgtttgg aatcagctag tatagtttct
     4621 gaactagtgg caggtttacg atagagaaaa tttttaaaaa tttctatgta tgattttcat
     4681 ttttttagtg agactgttgc cattatcaca ttcgaatcac acaaaataaa aaaatttcta
     4741 caagtacttg acaaatagat tgaaatatct taaaatgaaa atggttacag agttattaat
     4801 tatttaagct tcatgtcacc attaaaaatt gaaataaaag gatgttatca ctaatacaag
     4861 tgagcaggaa cctatttaat cacatcggaa gaagtttctt gatgttttta agtaggttcc
     4921 ttttatttta aaagggaaat tttatgatca taaaacgaat actaaaccac aatgccgtaa
     4981 ttgcgcaaag taaaaaagat atcgatattc ttctttttgg aaggggaata gcttttggaa
     5041 gaaaaactgg agataaagta aatccaattg atattgagaa aagttttttt ctcaaaaata
     5101 gagataatat gacccgtttt acagagatgt ttattaacgt tcctttggag ttggtgtaca
     5161 tcaccgaaaa aataattaac ctaggtaaaa taacattggg taataatttt gatgaaatta
     5221 tctatattaa tttaacggat catatttctt cgagcataga acgttataaa gaagggatta
     5281 ttatttcgaa tcccctacgc tgggaaatat cgaaatatta taaagaagaa tttgaacttg
     5341 ggaaaagggc tttacaaata ataaaaaaag agttaggtat tgaacttcca attgatgaag
     5401 ctgcattcat agcgctacat tttgttaatg ctaatttaga aaataatttt caagagtcgt
     5461 ataa
//
LOCUS       DBERDK010000098         5254 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.098, whole genome shotgun sequence.
ACCESSION   DBERDK010000098 DBERDK010000000
VERSION     DBERDK010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5254
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.098"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(617..1295)
                     /locus_tag="ACL73L_001941"
                     /pseudo
     CDS             complement(617..1295)
                     /locus_tag="ACL73L_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537001.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(1363..3185)
                     /locus_tag="ACL73L_001942"
                     /pseudo
     CDS             complement(1363..3185)
                     /locus_tag="ACL73L_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002917262.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1250 family conjugative relaxase"
     gene            complement(3172..3537)
                     /locus_tag="ACL73L_001943"
     CDS             complement(3172..3537)
                     /locus_tag="ACL73L_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HGS0772854.1"
                     /translation="MENRYRTNLKKVFLSDSELGQLKHCIDQSGCQSFSEYARRTLLD
                     PGMNFITIDTTGYQDLVFELKRIGNNINQIARSVNQSQLISSEELQDLKKGIADLIRE
                     VEKEFSIQAKKLREFHGHH"
     gene            complement(3542..3898)
                     /locus_tag="ACL73L_001944"
     CDS             complement(3542..3898)
                     /locus_tag="ACL73L_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1252 family conjugative relaxosome accessory
                     protein"
                     /protein_id="HGS0772855.1"
                     /translation="MPEQYRDIRKEVNLTANELEMIGVMMKNKGFEHFSSFARNKLLE
                     NELEMTAEKWFTFWRAQKLEQISRDVYEILILSRAEHQVTQEHVSILLTCVQELIQEI
                     GNSIPLSQDFRKKYMR"
     gene            complement(4792..>5254)
                     /locus_tag="ACL73L_001945"
     CDS             complement(4792..>5254)
                     /locus_tag="ACL73L_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HGS0772856.1"
                     /translation="FPEYNDEFRKELLKKLKRLRQRPTQSLIEEIASYLDVSITNYED
                     KLESQILLEILSNLDNKKMFDTNLMSNNLLMTTHASKGLAADTVVIFVEYLIDWRTGL
                     KFEDHYVAITRAKSKVIIIDNRTIYTSEINRLLSNNNGNFSFEDFIERRYI"
BASE COUNT         1576 a         1064 c          751 g         1863 t
ORIGIN      
        1 agttgttcac cccttcgctc cacttccact acagaagttt cctcactact atgggctcgg
       61 ctgacttctc atgattcgtt gttactacgc tttcggcgct catgagacct cacgggataa
      121 gccgtacatc tttcctcgtt tatacaagat atgaggacgt taaaaggtta caatgaaaat
      181 aggaaatctg acgcagagtc tttagactct aggaagattt atctttttca catagacctt
      241 agtccgaatt caattactct cgtgatttac gcatataggt tacgactacc tttttggact
      301 ttagagtttt aagccccctt atccgctgta tacgccttac tatcacgttc ctgttcgtag
      361 agcctcgatt tcgctatccc ttcctctgac gattcttatt tcctgcgtca gacttaaaac
      421 ggtctatccc cagaccgttt taatccgcta cctcacgata gtcaggcttg ggaatcgcta
      481 ttgggttcac cggtagcggg tgcccacgga ggacttacac ctcagatgca cgacatgccc
      541 gtcgtacctt aaaaagcctt ggaaaaaact gtttccaagg ctttctctat atttgtcctt
      601 attgaccaaa ccagttacat tgctttatcg tatctgtgat tttagtaata gcctcttcag
      661 tattattctg caaccataga tagcgacaaa cattataatt aaatttaata gaaaggttta
      721 attcttcgat tgtgtttaac ttgagttgat ttacttgata ctctaacttt tctctaattt
      781 cattaaaact ctctaaatct tcaatatcat aataaaaatt gaatagagta tttgcaactt
      841 gagggtaggt caagtcagtt acacttaact gagatagtac cttctccaat attgccacag
      901 cctcctcttt ttgcccatag aaataaaaat ctataagaga ttttatccac tccatataaa
      961 atttatttac cagagacaag cgatgtacct ttacactctc taattcatat atatttcaaa
     1021 gattcataat ttcgatttgt gataaatgtc tgggctaact ttttaaactc tgataactca
     1081 tcaatctctt ctacaatctg ctcattaaaa aaataatcta cactaacttt taacttctta
     1141 gacagagcat ataaaaagtc tgctcctgga gtaaattctc cattctctaa tcgactaatc
     1201 tgtccctgtt tacatatccc ctcagtcaat tctcgctgag acatttttaa ttcttttcgt
     1261 ctatttttta atctggttgc taatagtgtg cccaagtttg cattttcctt ttctattcga
     1321 tttcaaaaga tacaaattat atataagcaa tgactagata ggttattttt gaaaacctat
     1381 cccttcttct tttgaaattt tcttactcaa acgattgata aacttattca atttaaatac
     1441 gatgttctca tatctatcgc tctctaattc tatttggata cctaactcat tcaactcctt
     1501 ctcaacaaca tcaatgccaa ttttatcagt tatattcaac tttgaaagat tcattttcat
     1561 ttcttgctta ttttcaactg aagccagtaa atattctgct acttgattta aggttgtcac
     1621 tcgttcttga aaagcattga ttctcaattc agaagctgct aattcatgaa ctagttcatc
     1681 tttaattgta atataagagt gcttatccgt atccagctca agtaaagtat acacttcctc
     1741 aatcttctcc tttatcatct ccacagttgt tttcctgcgg aacggaattg tttgattttc
     1801 agagctgaat tggcgaatta atgttctacc tttcatatag caatttttct cggcatcatc
     1861 tttgtaatag acatagtagg agctcgtttc tctgataaaa accttcacct tatcctcctc
     1921 catatcgagc tccatgttag gcaaaaagat aagcccttcc tgacgaatac caaacttgac
     1981 cttgatgtaa ataccatcat ccactacttt ttcaatttga ttgagtgaca actctacctc
     2041 aaattcatga atagcatctc tatttctcct gaactcttga taggactccc aaaacttctc
     2101 ctcgcttggg agtctttttc ttttgagaat ctttcttctt gataaacttg aactaaatcc
     2161 tgaacttctg gagctttcaa atcttttctc ttctcaaaat aatctttgaa aaactctaca
     2221 tcatataagt ttttcttgtc tagttctgtt tctttcactt ctactcctgc aaattgaaaa
     2281 gaaacatgct tttgcttagg attgatattt aatccaaata gttttgcttt tttaatcaga
     2341 tcttctaagt tatcaacctt gggtaaaata aattccataa gctcttccat tttccttttt
     2401 gcgaagtaat tcttgaaaaa ttcttctgtg taagcttgct tgtgattgag tttattacca
     2461 cgcaccactt gtctcatagt tgagtccgtc ataaaaaagg tagcatgctt gtgactgaaa
     2521 tccatcttca catgtagtaa tggagcgttc tttttgaaat cctcaaaatc ggtagaattt
     2581 tccatcaaaa aatagagtcg ttgcttaagt tcatacttgt gattggtctt acgataaact
     2641 ttatactgat ggtaagaata gcgattctca ataatttttg cacctgcaat tttagaaaat
     2701 ctatctgaaa tcatgcgaag atttcgttcg actttataat tccactttag ttttttgtcc
     2761 gaattgttat caaccgaatt aacaatgata tgattatgga aatgattttt atcaacgtga
     2821 gtcgcaacga taaaacgaaa tttaccacca gttaactcct tcattgtctc atagccaatc
     2881 tgattgattt gtttaggagt gagatgatct tcgggtgaaa aggactgaat gatatgatga
     2941 gcatgaatta gtcgttgatt ttcctcatgc ctgtcgtggc gaaaatcgta aagcgtatca
     3001 ttactgataa aattttcatg atacatctgc accatctcct cataactagg aaaatccaga
     3061 aaattcctca tgccatagtg cgataccaat gcaagatttt cggtcttctc aggattgaga
     3121 atatacttga taattttttt gcgataacac tttccgtgaa cggcaaaatg cttagtgatg
     3181 accatgaaac tccctcaact ttttagcttg aatactaaac tctttctcta cctctcttat
     3241 caagtcagcg attccctttt tcaagtcttg caattcttca cttgaaatca actgagattg
     3301 attgacactt cgggcaatct ggttgatgtt attaccaatc ctcttcaact caaatactaa
     3361 atcttgataa ccagttgtat caatggtgat gaaattcata ccagggtcaa gtagagttcg
     3421 tcgagcatat tctgaaaaag attgacaacc gctttggtcg atacaatgtt tcaactgacc
     3481 caattcgcta tcagacagga aaactttctt tagattggtt cggtaacgat tttccatgtg
     3541 cctacctcat gtatttttta cggaagtctt gactaagagg aatggaattc cctatttctt
     3601 gaatcaattc ctgaacgcag gttaagagaa tggatacgtg ttcttgggtg acttgatgtt
     3661 ctgcccttga taagattaaa atctcataaa catcacgact gatttgttcc aacttttgag
     3721 ctcgccagaa ggtgaaccac ttttcagcag tcatttctaa ctcgttctcc agtaacttgt
     3781 ttctggcaaa tgaagaaaag tgttcaaatc ctttattttt catcattacg ccaatcattt
     3841 ctaattcatt tgcggtcaaa ttaacctctt tgcgaatgtc tctgtattgt tctggcatac
     3901 tacttcctca cttttatcta ttgtcggata attcccgacc ccgtgtcaac cacgggaaac
     3961 ttataaaaac aaaatctatc atcttgtaag gctttgcgag ctcagatatt gtgtccacaa
     4021 tatcccaaaa atcatatcgc cagctaatca aaacctacca gttttgacag ctaacgataa
     4081 gataacttgg tggctccgcc cccaaacccc cagtgaagat ttgaggtttt attttcttaa
     4141 gaatgatata agaatagcag agaaaatttg gaaaagatat cacaggagaa tgagaggatg
     4201 aaaaaaagag agcaaatgct ctcaatgaac tcatataaat atatatcaaa atactcttca
     4261 aaaacataag gttctaaaga ttatgtagaa aaagctttag caagtcttaa acactctgtt
     4321 gctctaactt atactggtcg atgctaaaat aatttaattc cttgaagtaa ttttccagct
     4381 tttaaatgat actcttgatt tttcatctcc tctaaacgac tatctacagc taaggcaaat
     4441 atattccata ttacattcca tcttcgtatc ttgttcttgt catataatct taaatgtcat
     4501 agagcatgga tttcatattt taataattag gtattcctac caatgctcga ctagctctat
     4561 ttatcccttc aacataaccc tttgagtcat tcaatatacc aaaattccag aaccttttag
     4621 aattattcgg tgtctagttg taaccgtaat actcgtaaaa tggctaatgt agtacaatca
     4681 tcttcatttt ttatttttca actcctgcac tattaaaatc aatgtgccaa ttaattgcag
     4741 aacctaaaac acctgtaata tcaaattgca atctatcttg atttagacta gtcaaatata
     4801 tcttctttct ataaaatctt caaaagaaaa attcccattg ttgttactta ataatctatt
     4861 gatttcacta gtataaattg ttcgattatc aataattatg acttttgatt ttgctctagt
     4921 aattgcaaca taatgatctt caaatttcaa tcctgtgcgc caatcaatta gatattctac
     4981 aaaaattact acagtgtctg cagccaaacc ttttgatgca tgagtagtca ttaataaatt
     5041 attactcatt aaatttgtat caaacatttt tttattatcc aaattactca aaatttccaa
     5101 cagtatttga ctttctaact tatcttcata atttgtaatt gaaacatcta aatacgaagc
     5161 tatttcctca ataagtgact gtgtaggtcg ttgacgaagc ctctttaatt tttttaaaag
     5221 ttctttccta aattcatcgt tatattcagg aaaa
//
LOCUS       DBERDK010000099         5222 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.099, whole genome shotgun sequence.
ACCESSION   DBERDK010000099 DBERDK010000000
VERSION     DBERDK010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5222
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.099"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(370..1098)
                     /locus_tag="ACL73L_001946"
     CDS             complement(370..1098)
                     /locus_tag="ACL73L_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGS0772857.1"
                     /translation="MSTKYLFIYNEIREKILCNKYTMNEQLPDEMTLAKQFACSRMTI
                     KKALDLLVSEGLIFRKRGQGTFVLSRGSSKRKLIVPERDIRGLTKISEDAHSTIDSRI
                     IHFKLEFANEFLAEKLQVALQSPVYNIYRLRIIDGKPYVLEQTYMSTDVIPGITEDIL
                     QKSIYNYIEGKLGLHIASATKILRASSSSENEQHYLQLLPTEPVFEVEQVAYLDNGTP
                     FEYSISRHRYDLFEFNSFALRHSS"
     gene            1256..2665
                     /locus_tag="ACL73L_001947"
     CDS             1256..2665
                     /locus_tag="ACL73L_001947"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HGS0772858.1"
                     /translation="MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPED
                     FFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFY
                     NAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
                     HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLELDG
                     KIGIILNLTPAYPRSNSPEDLEASRFTEDFFNKVFLNPAVKGTFPERLVKQLERDGVL
                     WSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMPDQYFKEYEWPERRM
                     NPYRGWEIFPKAIYDIAMIVKEEYDNIPWFISENGMGVENEARFIDENGVIDDVYRIE
                     FYEEHLRWLHKAIEEGSHCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKSS
                     GRWYRKVSDNNGFEVEIEE"
     gene            2683..3978
                     /locus_tag="ACL73L_001948"
     CDS             2683..3978
                     /locus_tag="ACL73L_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HGS0772859.1"
                     /translation="MKNSKFIDQFATFAGKLGNQIHLKTLRDAFVTVMPLYILAGLIV
                     LLNNTVFKWIFQGDTLTRFQYWGITIANGTLSISGMIIAVMVGYFLAKNRDFENPLAA
                     SMLSLVSLIVMMPNTVSVVPDGAKDAVNISGVLSFNNTGTGAMFAGVIVAIIATELFI
                     ELSNVKALQMNLGENIPPAVSRSFSVLLPVMTVISLFGVVSALLFNITGMNLISIITI
                     FIQEPIRHIGTSLIGVIIIYSLGNMLWLFGIHQAVIYSAILEPLLLINITENITAANN
                     GQAIPHIINLSQIQTFALMGGSGSTLCLLIATFLVSRNAVSKNVAKLSFGPGIFNINE
                     PVLFGYPIVYNISLAIPFITVPVLGILISYLATVTGFMSPAFIQVPWTTPVFLNAWLA
                     TAGDVRAVLVQFIIFALGVLLYIPFIKVNDKVVEQEMEG"
     gene            3983..4291
                     /locus_tag="ACL73L_001949"
     CDS             3983..4291
                     /locus_tag="ACL73L_001949"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGS0772860.1"
                     /translation="MKQILLVCNAGMSTSMLVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNIPVDTIDMRDYGMMNGAKVLEQALAWIGEIR
                     "
     gene            4288..4596
                     /locus_tag="ACL73L_001950"
     CDS             4288..4596
                     /locus_tag="ACL73L_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HGS0772861.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKVEE
                     AEDSLVEAHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIDLYQNK
                     "
BASE COUNT         1762 a          880 c          950 g         1630 t
ORIGIN      
        1 gcaaataaat tgggagattg aaattcattt ctagcaattt gttagaagtc acagtctact
       61 attctaacca cagtatacta tatgtgttac aagaaaagac tcaaaaactt ctcgtcagtg
      121 taagaatgtt tgaaacacta tttaaaacta aataactata agaagtagat caggtgaaca
      181 atttcagtac ctactctata agctatacga agatattttt caacatgatg gataaaacga
      241 tcactctttt aaagccatct tatttagaaa agagaaacca tttaagcata gatgggacct
      301 tcttgccctg ttttaaattt ttcttaaact agagtctgta aaagattggc ttcattttca
      361 cattttctcc taggaggaat gtcgtaatgc aaaagaatta aattcaaata aatcatagcg
      421 atgacgacta atcgagtact caaacggagt tccgttatcc aaataagcca cttgttctac
      481 ttcaaatacc ggttccgttg gaaggagctg caagtaatgt tgctcatttt ctgaactaga
      541 agaagctcgc aagatttttg tagcactggc aatatgcaat cctaactttc cttcaatgta
      601 attgtaaatc gatttttgta aaatatcttc agtaatacct ggaataacat cggtactcat
      661 ataagtttgt tccagaacat aaggtttacc gtcaataata cgcagacggt aaatattata
      721 aactggactc tgcaaagcga cctgtagttt ttctgctaaa aattcatttg caaattctaa
      781 tttgaagtga ataatcctcg agtcaattgt agaatgagca tcttcagata tttttgtcag
      841 tccccggata tctctttctg gaacgattaa ttttcttttt gagctgccac gagagagaac
      901 aaaggttccc tgcccacgtt ttctaaaaat taagccctca gaaactaaca agtctaaagc
      961 ttttttgatc gtcattcgac tacaggcaaa ctgtttagct aatgtcattt catcaggcaa
     1021 ttgttcgttc atggtatatt tattacaaag aatcttttca cgaatctcat tgtaaataaa
     1081 taaatatttt gtactcattt aatctggttc tctctataat attctaatag tagtataaca
     1141 aagttcaaga aatgaggcaa acacaagata tgtatcaaca atttttaaaa atgtctagac
     1201 aaattttaaa attatgctat actttatgta tagaataata gaaaggaggt tgagcatgaa
     1261 aaaaaagtta gtatttccta atctgttttg gtggggagct gcttctagcg gacctcagac
     1321 agaaggtcaa tatggaaaag tacatgaaaa tgtgatggac tactggttca aaacgcatcc
     1381 agaagatttt ttcgataatg tcggacctct tgtagccagt aacttttttc atacttacac
     1441 cgaagatttc cacttgatga aggaaattgg agttaattct ttccgcactt ccatccaatg
     1501 gagtcgactc atcaagaatt tagagacagg tgagcctgat ccaaaaggta ttgctttcta
     1561 caatgccatc attgaagaag ctaaaaagaa ccagatggat cttgtgatga atttacatca
     1621 ttttgattta ccagtggaac ttcttcaaaa atacggtggt tgggaaagca aacatgtagt
     1681 ggagttattc gtgaagtttg ccaagactgc tttcacatgc tttggagata aggttcatta
     1741 ctggacaact ttcaatgagc caatggtcat tccagaagca ggatacttat atgctttcca
     1801 ttatccaaat ctaaaaggaa agggaaaaga ggccgtacaa gtcatctata atctaaacct
     1861 tgctagtgca aaagtgattc aactatatcg ctcattagaa cttgatggaa agattgggat
     1921 tattttaaac ttgacacctg cttatccaag aagtaattct ccagaagact tagaagcaag
     1981 tcgatttaca gaagacttct ttaacaaagt cttcttgaat ccagctgtta aaggaacttt
     2041 cccagaaaga ttggtaaaac agctagagag agatggcgtg ttatggagtc ataccgaaaa
     2101 agagcttcaa ctgatgaaat caaatacggt tgattttctt ggagtaaact actaccatcc
     2161 aaaacgtgtt caagcacaag caaatcctga ggaatatcag acgccctgga tgccagacca
     2221 atacttcaaa gagtatgaat ggccggagcg tcgcatgaat ccatatcgtg gttgggaaat
     2281 ttttccgaaa gccatttatg atattgctat gattgtgaag gaagaatatg ataatattcc
     2341 atggtttatc agtgaaaacg gaatgggtgt tgaaaacgaa gcacggttta tcgatgaaaa
     2401 tggagttatc gatgacgtgt atcgtattga attttatgaa gaacatttaa gatggctaca
     2461 taaagccatt gaagagggaa gtcactgttt tggataccac gcttggaccg catttgattg
     2521 ctggtcttgg aataatgcat ataagaatcg ttacggattt atctccgttg atttagaaac
     2581 gcaaaagaga accatcaaga gctcaggaag atggtatcgc aaagtaagtg acaataacgg
     2641 ttttgaagta gaaattgagg agtaaagaga ggagatattc agatgaaaaa ctcaaaattt
     2701 atagatcaat ttgccacctt tgctggtaaa ctagggaacc aaattcattt aaaaacccta
     2761 agagatgcat tcgtaacagt aatgccatta tatattttgg caggtttaat cgttcttttg
     2821 aacaacacgg tatttaagtg gattttccaa ggggatacat taacaagatt ccaatattgg
     2881 ggaataacaa ttgcaaacgg tactttaagt atttcaggta tgattattgc tgtaatggtt
     2941 ggttatttct tagctaaaaa cagagatttc gaaaacccgt tagcagcatc aatgctatca
     3001 ttagtttctt taattgtgat gatgccaaat acagtttctg tagttcctga cggagcaaaa
     3061 gatgcggtaa acatttcagg tgttctttca ttcaacaaca caggtacagg cgcaatgttc
     3121 gccggggtta tcgtagcgat tattgcaaca gaattattca ttgaattatc aaacgttaaa
     3181 gctttacaaa tgaaccttgg tgaaaatatt ccaccagctg ttagtagatc atttagcgta
     3241 ttacttccag tcatgaccgt catctcctta tttggggttg tttcagcatt attattcaat
     3301 ataactggaa tgaacttaat ctcaatcatt acaatcttta ttcaagaacc aattcgtcat
     3361 attggtacaa gcttaatcgg ggtcattatt atttactctt taggaaatat gttatggcta
     3421 tttggtattc accaagcagt tatttacagt gccatcctag aaccattact attaattaac
     3481 attactgaaa acatcactgc agcaaataat ggacaagcca ttccacacat catcaaccta
     3541 tcacaaatac aaacattcgc tttaatgggt ggtagtggat ctacattatg tttattaata
     3601 gcaacattct tagtgagtcg caatgctgtc tctaaaaacg tggctaaatt atcttttgga
     3661 cctggtatct tcaatatcaa tgaaccagta ttattcggtt acccaatcgt ttataacatt
     3721 tcattagcta ttccatttat cacagttcca gtccttggta ttttaatcag ctacttagca
     3781 acagttacag gattcatgag tcctgcattt atacaagttc cttggactac accagtattc
     3841 ttaaatgcat ggttagcaac agcaggggac gtgagagcag ttctagttca attcatcatc
     3901 tttgcacttg gagttcttct atacattcca tttatcaaag ttaatgacaa agttgttgaa
     3961 caagaaatgg aaggttaaga ttatgaaaca aattttatta gtatgtaatg cgggaatgtc
     4021 gacaagtatg cttgttaaaa aaatgcaaca aagtgcgact gaacgtggga ttgaaatctc
     4081 tatccaagca aaatcaatga cagaagctaa aaagaatatc catgaagcag atgtcattct
     4141 aattgggcca caaattcgtt atgagttact tgctgtgaaa gaaattgcag gaaatatccc
     4201 tgtagatacc attgatatgc gggattacgg aatgatgaat ggagcaaaag ttctggaaca
     4261 agcacttgca tggataggag aaataagatg acagaagaaa tggaatatat ctgttttcag
     4321 ctcattgcca atagtggagc agccaaatca tcctttattg aagccatcca actagctaag
     4381 gcaggaaatt taaaagaagc gaaaattaaa gttgaagaag cggaagactc cttagtagaa
     4441 gcgcacaaga ttcactctaa tctcatacaa aaagaagcta ctggagaaaa aataggattc
     4501 tccttgctat ttatgcatgc agaagaccaa atggcttcaa cagagattat tcagttgcta
     4561 tctaaagaat tcatcgatct ctatcaaaat aaataggaac actgtcattc ccatctgtct
     4621 ttattaatga cgactacatt gtaggaacct ccaaaagttg gacaaaatga ttgaaaggat
     4681 tggatcctgc attacacaag atccaatcct tttcttttac tctagttttt ataatatgtt
     4741 gaatctagaa tagtccactg tagtttctaa aaaattgctg gaaatggatg ttaagctcca
     4801 atttatttgt ttatatctta tttcagttta ctatactagc ttattattct agtgaaaaat
     4861 tgaatgagcc actatttatt taatatgtat gatgagggaa tgactcaaaa acttcttgtc
     4921 gatataagaa tatctgagat agtattttaa aacgaaagga ctataaaagc tcatttcctt
     4981 cgcacttatt ttatagagat ctatcattat actctttaga aatcaaaaag caacacacct
     5041 tgtcatcgtt ttatcgtaaa tctgttaatg agggggctag tcttatctgc aatctcaaat
     5101 cagtgttttg agctgaatgg cactcgtttt ctagtttgct ctttgatttt aattgggcct
     5161 tagtaaatac tactcttaaa ttaaactata gtgaattgaa ataagatgtg aacaactcta
     5221 tc
//
LOCUS       DBERDK010000100         4761 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.100, whole genome shotgun sequence.
ACCESSION   DBERDK010000100 DBERDK010000000
VERSION     DBERDK010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4761
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.100"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            51..1596
                     /locus_tag="ACL73L_001951"
     rRNA            51..1596
                     /locus_tag="ACL73L_001951"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1648..1720
                     /locus_tag="ACL73L_001952"
     tRNA            1648..1720
                     /locus_tag="ACL73L_001952"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1681..1683,aa:Ala,seq:tgc)
     gene            1845..4746
                     /locus_tag="ACL73L_001953"
     rRNA            1845..4746
                     /locus_tag="ACL73L_001953"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         1326 a          977 c         1412 g         1046 t
ORIGIN      
        1 gaaaaaaaca ataaatctgt cagtgacaga aatgagtgag aactcaaact tttaatgaga
       61 gtttgatcct ggctcaggac gaacgctggc ggcgtgccta atacatgcaa gtagaacgct
      121 gaaggaggag cttgcttctc tggatgagtt gcgaacgggt gagtaacgcg taggtaacct
      181 gcctggtagc gggggataac tattggaaac gatagctaat accgcataag agtagatgtt
      241 gcatgacatt tgcttaaaag gtgcacttgc atcactacca gatggacctg cgttgtatta
      301 gctagttggt ggggtaacgg ctcaccaagg cgacgataca tagccgacct gagagggtga
      361 tcggccacac tgggactgag acacggccca gactcctacg ggaggcagca gtagggaatc
      421 ttcggcaatg gacggaagtc tgaccgagca acgccgcgtg agtgaagaag gttttcggat
      481 cgtaaagctc tgttgtaaga gaagaacgag tgtgagagtg gaaagttcac actgtgacgg
      541 tatcttacca gaaagggacg gctaactacg tgccagcagc cgcggtaata cgtaggtccc
      601 gagcgttgtc cggatttatt gggcgtaaag cgagcgcagg cggttagata agtctgaagt
      661 taaaggctgt ggcttaacca tagtaggctt tggaaactgt ttaacttgag tgcaagaggg
      721 gagagtggaa ttccatgtgt agcggtgaaa tgcgtagata tatggaggaa caccggtggc
      781 gaaagcggct ctctggcttg taactgacgc tgaggctcga aagcgtgggg agcaaacagg
      841 attagatacc ctggtagtcc acgctgtaaa cgatgagtgc taggtgttag accctttccg
      901 gggtttagtg ccgtagctaa cgcattaagc actccgcctg gggagtacga ccgcaaggtt
      961 gaaactcaaa ggaattgacg ggggcccgca caagcggtgg agcatgtggt ttaattcgaa
     1021 gcaacgcgaa gaaccttacc aggtcttgac atccctctga ccgctctaga gatagagttt
     1081 tccttcggga cagaggtgac aggtggtgca tggttgtcgt cagctcgtgt cgtgagatgt
     1141 tgggttaagt cccgcaacga gcgcaacccc tattgttagt tgccatcatt cagttgggca
     1201 ctctagcgag actgccggta ataaaccgga ggaaggtggg gatgacgtca aatcatcatg
     1261 ccccttatga cctgggctac acacgtgcta caatggctgg tacaacgagt cgcaagccgg
     1321 tgacggcaag ctaatctctt aaagccagtc tcagttcgga ttgtaggctg caactcgcct
     1381 acatgaagtc ggaatcgcta gtaatcgcgg atcagcacgc cgcggtgaat acgttcccgg
     1441 gccttgtaca caccgcccgt cacaccacga gagtttgtaa cacccgaagt cggtgaggta
     1501 accgtaagga gccagccgcc taaggtggga tagatgattg gggtgaagtc gtaacaaggt
     1561 agccgtatcg gaaggtgcgg ctggatcacc tcctttctaa ggataaggaa ctgcgcattg
     1621 gtcttgttta gtcttgagag gtcttgtggg gccttagctc agctgggaga gcgcctgctt
     1681 tgcacgcagg aggtcagcgg ttcgatcccg ctaggctcca ttggtgagag atcaccaagt
     1741 aatgcacatt gaaaattgaa tatctatatc aaatagtaac aagaaaataa accgaaaacg
     1801 ctgtagtatt aataagagtt tatgactgaa aggtcagaaa ataaggttaa gttaataagg
     1861 gcgcacggtg gatgccttgg cactaggagc cgacgaagga cgtgacaaac gacgatatgc
     1921 cttgggtagc tgtaagtaag cgatgatcca gggatttccg aatgggggaa cccaacaggt
     1981 aatacctgtt acccacatct gttaaggatg tgaggaggaa gacgcagtga actgaaacat
     2041 ctaagtagct gcaggaagag aaagcaaaag cgattgcctt agtagcggcg agcgaaacgg
     2101 cagaagggca aaccgaagag tttactcttc ggggttgtag gactgcaatg tggactcaaa
     2161 gattatagaa gaatgatttg ggaagatcag ccaaagagag taatagcctc gtatttaaaa
     2221 tagtctttgt acctagcagt atcctgagta cggcgggaca cgtgaaatcc cgtcggaatc
     2281 tgggaggacc atctcccaac cctaaatact ccctagtgac cgatagtgaa ccagtaccgt
     2341 gagggaaagg tgaaaagcac cccgggaggg gagtgaaata gaacctgaaa ccgtgtgcct
     2401 acaacaagtt cgagcccgtt aatgggtgag agcgtgcctt ttgtagaatg aaccggcgag
     2461 ttacgttatg atgcgaggtt aagttgaaga gacggagccg tagggaaacc gagtctgaat
     2521 agggcgcctt agtatcatga cgtagacccg aaaccatgtg acctacccat gagcaggttg
     2581 aaggtgcggt aagacgcact ggaggaccga accagggcac gttgaaaagt gcttggatga
     2641 cttgtgggta gcggagaaat tccaaacgaa cttggagata gctggttctc tccgaaatag
     2701 ctttagggct agcgtcgaca ttagagattc ttggaggtag agcactgttt gggtgagggg
     2761 tccatcccgg attaccaatc tcagataaac tccgaatgcc aatgaattat ggtcggcagt
     2821 cagactgcga gtgctaagat ccgtagtcga aagggaaaca gcccagacca ccagctaagg
     2881 tcccaaaata attgttaagt ggaaaaggat gtggggttgc acagacaact aggatgttag
     2941 cttagaagca gctattcatt caaagagtgc gtaatagctc actagtcgag tgaccctgcg
     3001 ccgaaaatgt accggggcta aaacaattta ccgaagctgt ggataccttt ataggtatgg
     3061 taggagagcg ttctatgtgt gatgaaggta taccgtgagg agtgctggaa cgcatagaag
     3121 tgagaatgcc ggtatgagta gcgaaagaca ggtgagaatc ctgtccaccg taagactaag
     3181 gtttccaggg gaaggctcgt ccgccctggg ttagtcggga cctaaggaga gaccgaaagg
     3241 tgtatccgat ggacaacagg ttgatattcc tgtactagag tatgtagtga tggagggacg
     3301 cagtaggcta actaaagcag acgattggaa gagtctgtct aagcagtgag gtgtgaattg
     3361 agtcaaatgc ttaattctat aacattgagc tgtgatgggg agcgaagttt agtagcgaag
     3421 ttagtgacgt cacactgcca agaaaagctt ctagcgttta aacatactct acccgtaccg
     3481 caaaccgaca caggtagtcg aggcgagtag cctcaggtga gcgagagaac tctcgttaag
     3541 gaactcggca aaatgacccc gtaacttcgg gagaaggggt gctgacttta agtcagccgc
     3601 agtgaatagg cccaagcaac tgtttatcaa aaacacagct ctctgctaaa tcgtaagatg
     3661 atgtataggg ggtgacgcct gcccggtgct ggaaggttaa gaggagtgct tagcgtaagc
     3721 gaaggtatga attgaagccc cagtaaacgg cggccgtaac tataacggtc ctaaggtagc
     3781 gaaattcctt gtcgggtaag ttccgacccg cacgaaaggc gtaatgattt gggcactgtc
     3841 tcaacgagag actcggtgaa attttagtac ctgtgaagat gcaggttacc cgcgacagga
     3901 cggaaagacc ccatggagct ttactgcagt ttgatattga gtgtctgtac cacatgtaca
     3961 ggataggtag gagtctaaga gatcgggacg ccagtttcga aggagacgct gttgggatac
     4021 tacccttgtg ttatggccac tctaacccag ataggtgatc cctatcggag acagtgtctg
     4081 acgggcagtt tgactggggc ggtcgcctcc taaaaggtaa cggaggcgcc caaaggttcc
     4141 ctcagaatgg ttggaaatca ttcgcagagt gtaaaggtat aagggagctt gactgcgaga
     4201 gctacaactc gagcagggac gaaagtcggg cttagtgatc cggtggttcc gtatggaagg
     4261 gccatcgctc aacggataaa agctaccctg gggataacag gcttatctcc cccaagagtt
     4321 cacatcgacg gggaggtttg gcacctcgat gtcggctcgt cgcatcctgg ggctgtagtc
     4381 ggtcccaagg gttgggctgt tcgcccatta aagcggcacg cgagctgggt tcagaacgtc
     4441 gtgagacagt tcggtcccta tccgtcgcgg gcgtaggaaa tttgagagga tctgctccta
     4501 gtacgagagg accagagtgg acttaccgct ggtgtaccag ttgtcttgcc aaaggcatcg
     4561 ctgggtagct atgtagggaa gggataaacg ctgaaagcat ctaagtgtga aacccacctc
     4621 aagatgagat ttcccatgat tatatatcag taagagccct gagagatgat caggtagata
     4681 ggttagaagt ggaagtgtgg cgacacatgt agcggactaa tactaatagc tcgaggactt
     4741 atccaaagta actgagaata t
//
LOCUS       DBERDK010000101         4679 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.101, whole genome shotgun sequence.
ACCESSION   DBERDK010000101 DBERDK010000000
VERSION     DBERDK010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4679
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.101"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..370
                     /locus_tag="ACL73L_001954"
     CDS             <1..370
                     /locus_tag="ACL73L_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HGS0772862.1"
                     /translation="GAMATGWAKVNGSWYYLNANGSMATGWLQYNGSWYYLNANGAMA
                     TGWAKVNGSWYYLNANGSMATGWVKDGDTWYYLEASGAMKASQWFKVSDKWYYVNGLG
                     ALAVNTTVDGYKVNANGEWV"
     gene            771..1892
                     /gene="mnmA"
                     /locus_tag="ACL73L_001955"
     CDS             771..1892
                     /gene="mnmA"
                     /locus_tag="ACL73L_001955"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HGS0772863.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAITLGADYVATGHYARVARDEDGTVHMLRGVDNGKDQTYFLSQLS
                     QEQLQKTMFPLGHLEKPEVRKLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKALSKNILYVGQ
                     GFYHDSLMSTSLEASQVHFTCDMPEEFTLECTAKFRYRQPDSKVTVHVKGDKAEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
     gene            2033..2488
                     /locus_tag="ACL73L_001956"
     CDS             2033..2488
                     /locus_tag="ACL73L_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HGS0772864.1"
                     /translation="MTQQDFRTKVDNTVFGVRATALIVQNHKLLVTKDKGKYYTIGGA
                     IQVNESTEDAAVREVKEELGVKAQAGQLAFVVENRFEVDGVSYHNIEFHYLVDLLEDA
                     PLTMQEDEKRQPCEWIDLDKLEDIQLVPAFLKTALPDWEGQLRHIHLEE"
     gene            2498..4411
                     /gene="mnmG"
                     /locus_tag="ACL73L_001957"
     CDS             2498..4411
                     /gene="mnmG"
                     /locus_tag="ACL73L_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HGS0772865.1"
                     /translation="MTYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLTLRQTMIDEILVEDGKVVGVRTATHQEYAAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDEVPNHFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQGKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEMGREIGLVDDERWARFEIKKNQFDNEMKRLDSIKLKPVKETNAKVEEMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEDLDDKIIELIETEIKYEGYISKAMDQVAKMK
                     RMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
BASE COUNT         1345 a          951 c         1153 g         1230 t
ORIGIN      
        1 cggcgctatg gcaacaggtt gggctaaagt caacggttca tggtactacc tcaacgctaa
       61 cggttcaatg gcaacaggtt ggctccaata caacggttca tggtactacc tcaacgctaa
      121 cggtgctatg gcaacaggtt gggctaaagt caacggttca tggtactacc taaacgctaa
      181 cggttcaatg gcaacaggtt gggtgaaaga tggagatacc tggtactatc ttgaagcatc
      241 aggtgctatg aaagcaagcc aatggttcaa agtatcagat aaatggtact atgtcaatgg
      301 cttaggtgcc cttgcagtca acacaactgt agatggctat aaagtcaatg ccaatggtga
      361 atgggtttaa gccgattaaa ttaaatcatg ttaagaacat ttgacatttt aattttgaaa
      421 caaagataag gttcgattga atagatttat gttcgtattc tttaggtacc tatcttatga
      481 tttcaggaaa tgtcattaaa aaaacgactc cttttctcta acttgaaaaa tagattagag
      541 aaaatgggtt gttttattta ttatagttat ttgaatgaag ataagaataa ggtatactca
      601 catcatttac ataatctgta tattgactat aagttttaaa aaacaatttt taagctcttc
      661 cttgtcttct ctaaccaagc gtgttataat gaatactgct caagcgacct tcaatcgtga
      721 agcacacacg accttcaatc gtgaataaac gaatagatgg gagacttacc atgagtgata
      781 actctaaaac acgtgttgtc gtggggatga gtggtggtgt tgattcgtcg gtgacggctc
      841 ttttgctcaa ggagcagggc tacgatgtga tcggtatctt catgaagaac tgggatgaca
      901 cagatgaaaa cggcgtctgt acggcgaccg aagattacaa ggatgtggtt gcggtggcag
      961 accagattgg cattccctac tactctgtca attttgaaaa agagtactgg gaccgcgttt
     1021 ttgagtattt cctagcggaa taccgtgcag ggcgcacgcc aaatccggac gttatgtgca
     1081 acaaggaaat caagttcaag gcctttttgg actatgccat aaccttgggg gcagactatg
     1141 tagcgactgg gcattatgct cgagtggcgc gtgatgagga tggtaccgtt cacatgcttc
     1201 gtggcgtgga caatggcaag gatcagacct atttcctcag ccaactttcg caagaacaac
     1261 ttcaaaaaac catgttccca ctaggacatt tggaaaagcc tgaagttcga aaactagcag
     1321 aagaagcagg tctttcgact gctaagaaga aagactcgac agggatttgc tttatcggag
     1381 aaaagaactt taaaaacttt ctcagcaact acctgccagc tcagcctggt cgcatgatga
     1441 ctgtggatgg tcgcgatatg ggcgagcatg caggtctgat gtactatacg attggtcagc
     1501 gtggcgggct tggtatcggt gggcaacacg gtggtgacaa tgccccttgg ttcgttgtcg
     1561 gaaaagctct aagcaagaat attctctatg taggacaagg attctaccat gattcgctca
     1621 tgtcaaccag cctagaggct agccaagtcc actttacttg tgatatgccc gaggagttta
     1681 cgctagaatg tacggctaaa ttccgttacc gtcagcctga ctctaaggtg accgtccatg
     1741 tcaaaggaga caaggcagag gtcatctttg cggaaccaca acgcgccatt acaccaggac
     1801 aggcagttgt cttttacgat ggcgaagagt gtctaggtgg cggtttgatt gacaatgctt
     1861 accgcgatgg acaagtttgt cagtacattt agattgacaa attttctcaa tttgctacaa
     1921 taataacagc aatagaaatg atggtcaaag ctcatggatg ttgcaggctt ttttgtcctg
     1981 cacttctttg tagttttgac tgtttttgtg tcgtttaagg gaaaggacaa gaatgactca
     2041 acaagacttt cggacaaaag tagacaatac ggtttttggt gttcgggcga cagccttgat
     2101 tgtccaaaat cacaagcttc tagttaccaa agacaagggc aagtattaca ctattggagg
     2161 tgcgattcaa gtcaatgaaa gcacggaaga cgcggcagtc cgtgaagtga aggaagaact
     2221 gggtgtcaaa gctcaagctg ggcagctagc ttttgtggtt gaaaatcgtt ttgaagtgga
     2281 cggcgtttcc tatcacaata tcgagtttca ttatctggtg gatttgcttg aagatgcccc
     2341 gttgaccatg caggaagatg agaaaaggca gccctgtgag tggattgact tggataagct
     2401 tgaggatata cagctagttc cagccttttt aaaaacggcc ctaccagatt gggaaggcca
     2461 actaagacac attcatcttg aggaatagga gagaaacatg acttatcatt ttactgaaga
     2521 atacgatatt attgtaattg gtgcgggaca cgctggggtt gaggcttcct tggccgctag
     2581 ccgtatgggc tgtaaggtcc tgcttgcgac catcaatatt gaaatgctgg ctttcatgcc
     2641 ttgtaatccc tctatcggtg gttctgccaa ggggattgtc gtgcgtgaag tcgatgccct
     2701 cggtggcgag atggccaaaa ccattgacaa gacttacatc cagatgaaga tgctaaacac
     2761 agggaagggg ccagctgtcc gtgcccttcg tgcgcaggct gacaaggaac tttactctaa
     2821 ggagatgcgc aagacggttg aaaaccaaga aaatctgacc cttcgtcaaa ccatgattga
     2881 tgagattttg gtggaagatg gcaaggttgt cggtgtgcgt acagccaccc atcaagaata
     2941 tgctgctaag gctgttattg tgacgacagg gactgctctc cgtggggaaa ttatcatcgg
     3001 agacctcaag tactcatcag gtcctaacca cagcttggct tctattaacc tagctgataa
     3061 tctcaaggaa ttaggactcg aaatcggtcg tttcaagaca ggaacccctc cacgtgtcaa
     3121 ggcttcttct atcaactacg acgtgacaga aattcagcca ggagacgaag tgcctaatca
     3181 tttctcatac acttcacgtg atgaggatta tgtcaaagat caagtgccat gctggttgac
     3241 ctacaccaat ggtaccagtc atgagattat ccaaaacaac ctccaccgtg cgcctatgtt
     3301 tacaggtgtg gtcaagggag tggggcctcg ttactgtccg tcgattgaag acaagattgt
     3361 gcgctttgcg gacaaggaac gtcaccaact cttccttgag ccagaagggc gcaatactga
     3421 ggaagtctat gttcaaggac tttcaaccag tctgcctgag gatgtccagc gtgacttggt
     3481 tcattccatc aaaggtttgg aaaatgcaga gatgatgcgg acaggttatg ctattgagta
     3541 tgatatggtc ttgcctcatc agttgcgtgc gactctggaa accaagaaaa tctcaggtct
     3601 cttcactgct ggtcagacaa atggaacgtc aggttacgaa gaggcagcag gccaagggat
     3661 tatcgcgggt atcaatgcgg ctctgaaaat ccaaggtaag cctgagttga ttttgaagcg
     3721 aagtgacggt tatatcgggg tcatgatcga cgacttggtg accaagggaa ccattgaacc
     3781 ttaccgtctc ttgaccagtc gtgctgaata tcgtctcatt cttcgccatg acaatgctga
     3841 tatgcgcttg actgagatgg gacgcgagat tggccttgtg gatgatgaac gctgggctcg
     3901 ttttgaaatc aagaaaaatc aatttgataa tgagatgaaa cgcctagaca gtatcaaact
     3961 caagccagtc aaggaaacca atgctaaggt tgaggaaatg ggcttcaagc cgttgacaga
     4021 tgcggtgaca gccaaggaat tccttcgccg tccagaagtt tcttaccaag atgtggtggc
     4081 cttcatcgga ccagctgcag aagacttgga tgacaagatt atcgaattga ttgaaacaga
     4141 aatcaagtat gaaggctata tttccaaagc catggaccag gttgccaaga tgaaacgcat
     4201 ggaagaaaaa cgcattccgg ccaatatcga ctgggatgac attgattcta tcgcaaccga
     4261 agcccgtcag aagttcaaac tcatcaatcc agaaaccatc ggccaagcca gccgtatttc
     4321 gggagtaaac ccagcagata tttctatttt gatggtgtat ctggaaggta aaaatcgtag
     4381 tatttctaaa actcttcaaa aatcaaaatg atacgtcgta ggcttcttac gaatgagttc
     4441 aaagcttggc tttgattcat ctccaacctc ccatagttcc ccgaactatg ggagctaact
     4501 caagttatgc attttcctag ccttctggtc tgattttcat tgagttttaa tctaaaaaga
     4561 aagcatagag aaaaacagct ccgaagacgg ggctgttttg ttgatttgta ctcaatgaaa
     4621 atcaaagagc aaactaggaa gctagccgta ggttgctcaa agcactgctt tgaggttgt
//
LOCUS       DBERDK010000102         4505 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.102, whole genome shotgun sequence.
ACCESSION   DBERDK010000102 DBERDK010000000
VERSION     DBERDK010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4505
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.102"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            163..816
                     /gene="rr06"
                     /locus_tag="ACL73L_001958"
     CDS             163..816
                     /gene="rr06"
                     /locus_tag="ACL73L_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RR06"
                     /protein_id="HGS0772866.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSALGDETTQSQVFDLYADDHVTKPF
                     SLVLLVKRIKALIRRYYVIEDLWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
                     QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL"
     gene            813..2153
                     /gene="hk06"
                     /locus_tag="ACL73L_001959"
     CDS             813..2153
                     /gene="hk06"
                     /locus_tag="ACL73L_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HK06"
                     /protein_id="HGS0772867.1"
                     /translation="MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGE
                     KFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDG
                     KLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILLLYLPYTFLVTIAFSFVFS
                     YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGEVGKQINGMYEHLLKV
                     IHELESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIA
                     KSINKIDQMSHLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIEN
                     QLTDATRVVMSLRALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCAPLSDQE
                     LEHLFDIFYHSQIVTDKDESSGLGLYIVNNILESYQMDYSFLPYEHGMEFKISL"
     gene            2236..2763
                     /locus_tag="ACL73L_001960"
     CDS             2236..2763
                     /locus_tag="ACL73L_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HGS0772868.1"
                     /translation="MVLAIILVTFLIRLIFLKRSIENEKRILSNGGKEFGVENTKRLT
                     MAHIVFYLSCLVEAMVHKTIFDGMGMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKYVDHILFRTVKHPNYFLNILPELIGLTLLSHAYVTFVLVFPVYAVILYRRIAEEE
                     KLLHEVIIPNGSIKR"
     gene            2842..>4505
                     /gene="pspC"
                     /locus_tag="ACL73L_001961"
     CDS             2842..>4505
                     /gene="pspC"
                     /locus_tag="ACL73L_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein PspC,
                     choline-binding form"
                     /protein_id="HGS0772869.1"
                     /translation="MFASKSERKVHYSIRKFSIGVASVAVASLFLGGVVHAEGVRSEN
                     TPKVTSSGDEVDEYIKKMLSEIQLDKRKHTHNFALNLKLSRIKTEYLYKLKVNVLEEK
                     SKAELTSKTKKEVDAAFEKFKKDTLKLGEKVAEAQKKVEEAKKKAKDQKEEDHRNYPT
                     NTYKTLELEIAESDVKVKEAELELLKEEAKTRNEDTINQAKAKVKSEQAEATRLKKIK
                     TDREQAEATRLENIKTDREKAEEAKRKAEAEEVKDKLKRRTKRAVPGEPATPDKKEND
                     AKSSDSSVGEETLPSPSLKSGKKVAEAQKKVAEAEKKAKDQKEEDRRNYPTNTYKTLD
                     LEIAESDVKVKEAELELVKEEAKESRNEEKVKQAKAKVESKKAEATRLENIKTDRKKA
                     EEAKRRAAEEDKVKEKPAEQPQPAPAPQPEKPTEEPENPAPAPKPENPAEQPKAEKPA
                     DQQAEEDYARRSEEEYNRLTQQQPPKTEKPAQPSTPKTGWKQENGMWYFYNTDGSMAT
                     GWLQNNGSWYYLNSNGAMATGWLQNNGSWYYLNANGDMATGWLQYNG"
BASE COUNT         1623 a          676 c          961 g         1245 t
ORIGIN      
        1 agctgcaaga tgaatgattt tcttgtattg acgttgttgt tgacaaaaag tagcggataa
       61 atgaaatcca ttccattatc atagatgata ggctggtagg aaattttcaa atagcataca
      121 ggaaatagat gtatggagtt ctggtagtag aaagggagag agatgaacat tttagttgca
      181 gatgacgagg aaatgattag agaaggaatt gcagcatttc tgacagaaga gggttatcat
      241 gtcattatgg ctaaggatgg acaagaggtc ttggaaaaat ttcaagatct ccctatccat
      301 ctcatggtac tggatttaat gatgcctagg aagagtggtt ttgaagtgtt aaaagaaatc
      361 aatcaaaagc acgatattcc tgtcatcgtc ttgagtgctc tgggagatga aactactcag
      421 tcacaggtat ttgatctcta tgctgatgat catgtgacaa aacctttttc tttggtactg
      481 cttgtcaagc gtattaaggc gcttatcaga cgttactacg tcatagagga tctttggcga
      541 tatcaggatg taacagtgga ttttacctct tacaaagcac attataaaaa tgaagaaatt
      601 gatctcaaac caaaggaatt actggtacta aagtgtttga ttcagcataa aaatcaagtt
      661 ttaagtagag agcagatatt ggaagaaatt tcaaaagatg tagctgattt accttgtgat
      721 agggtcgttg atgtctatat tcgtactctt cgcaaaaaat tagctttaga ttgtatcgtg
      781 actgtgaaaa atgttgggta taagattagc ttatgataaa aaatcctaaa ttattaacca
      841 agtctttttt aagaagtttt gcaattctag gtggtgttgg tctagtcatt catatagcta
      901 tttatttgac ctttcctttt tattatattc aactggaggg ggaaaagttt aatgagagcg
      961 caagagtgtt tacggagtat ttaaagacta agacatctga tgaaattcca agcttactcc
     1021 agtcttattc aaagtccttg accatatctg ctcaccttaa aagagatatt gtagataagc
     1081 ggctccctct tgtgcatgac ttggatatta aagatggaaa gctatcaaat tatatcgtga
     1141 tgttagatat gtctgttagt acagcagatg gtaaacaggt aaccgtgcaa tttgttcacg
     1201 gggtggatgt ctacaaagaa gcaaagaata ttttgctttt gtatctccca tatacatttt
     1261 tggttacaat tgctttttcc tttgtttttt cttattttta tactaaacgc ttgctcaatc
     1321 ctctttttta catttcagaa gtgactagta aaatgcaaga tttggatgac aatattcgtt
     1381 ttgatgaaag taggaaagat gaagttggtg aagttggtga agttggaaaa cagattaatg
     1441 gtatgtatga gcacttgttg aaggttattc atgagttgga aagtcgtaat gagcaaattg
     1501 taaaattgca aaatcaaaag gtttcctttg tccgcggagc atcacatgag ttgaaaaccc
     1561 ctttagccag tcttagaatt atcctagaga atatgcagca taatattgga gattacaaag
     1621 atcatccaaa atatattgca aagagtataa ataagattga ccagatgagc cacttattgg
     1681 aagaagtact ggagtcttct aaattccaag agtggacaga gtgtcgtgag accttgactg
     1741 ttaagccagt tttagtagat attttatcac gttatcaaga attagctcat tcaataggtg
     1801 ttacaattga aaatcaattg acagatgcta ccagggtcgt catgagtctt agggcattgg
     1861 ataaggtttt gacaaacctg attagtaatg caattaaata ttcagataaa aatgggcgcg
     1921 taatcatatc cgagcaagat ggctatctct ctatcaaaaa tacatgtgcg cctctaagtg
     1981 accaagaact agaacattta tttgatatat tctatcattc tcaaatcgtg acagataagg
     2041 atgaaagttc cggtttgggt ctttacattg tgaataatat tttagaaagc tatcaaatgg
     2101 actatagttt tctcccttat gaacacggta tggaatttaa gattagcttg tagacagatt
     2161 agttttttat taaagttcat atagggttaa cataagtgtg ttattctttg tgtagataaa
     2221 agaaaggata ctaatatggt attagcgatt attttagtaa cattccttat tcgattgatt
     2281 tttttaaagc gttcgataga gaatgagaaa cgaatcctta gcaatggcgg gaaagaattt
     2341 ggagttgaga atacaaaacg attaactatg gctcatattg ttttttatct ctcttgcttg
     2401 gttgaggcaa tggtgcacaa gacaattttt gatggcatgg gcatggttgg tttagtcttg
     2461 cttatttttt ctatgctgat gttgatgttg gtgattcact tgttgggaga tatttggaca
     2521 gtgaagctta tgcttgtcaa taatcacaaa tatgtagatc atatcttgtt taggacagta
     2581 aaacacccta attacttttt aaatatttta cctgagttga ttggcttgac cttgttgagt
     2641 catgcttatg tgacttttgt tttagttttt ccagtttatg cagttatttt gtatcgacga
     2701 atagctgaag aggaaaagct attacatgaa gttataatcc caaatggaag cataaagaga
     2761 taaatacaaa attcgattta tatacagttc atattgaagt gatatagtaa ggttaaagaa
     2821 aaaatataga aggaaataat tatgtttgca tcaaaaagcg aaagaaaagt acattattca
     2881 attcgtaaat ttagtattgg agtagctagt gtagctgttg ctagcttgtt cttaggagga
     2941 gtagtccatg cagaaggggt tagaagtgag aataccccca aggttacatc tagtggggat
     3001 gaagtcgatg aatatataaa aaaaatgttg agtgagatcc aattagataa aagaaaacat
     3061 acccacaatt tcgccttaaa cctaaagttg agcagaatta aaacggagta tttgtataaa
     3121 ttaaaagtta atgttttaga agaaaagtca aaagctgagt tgacgtcaaa aacaaaaaaa
     3181 gaggtagacg cagcttttga gaagtttaaa aaagatacat tgaaactagg agaaaaggta
     3241 gcagaagctc agaagaaggt tgaagaagct aagaaaaaag ccaaggatca aaaagaagaa
     3301 gatcaccgta actacccaac caatacttac aaaacgcttg aacttgaaat tgctgagtcc
     3361 gatgtgaaag ttaaagaagc ggagcttgaa ctattgaaag aggaagctaa aactcgaaac
     3421 gaggacacaa ttaaccaagc aaaagcgaaa gttaagagtg aacaagctga ggctacaagg
     3481 ttaaaaaaaa tcaagacaga tcgtgaacaa gctgaggcta caaggttaga aaacatcaag
     3541 acagatcgtg aaaaagcaga agaagctaaa cgaaaagcag aagcagaaga agttaaagat
     3601 aaactaaaga ggcggacaaa acgagcagtt cctggagagc cagcaacacc tgataaaaaa
     3661 gaaaatgatg cgaagtcttc agattctagc gtaggtgaag aaactcttcc aagcccatcc
     3721 ctgaaatcag gaaaaaaggt agcagaagct cagaagaagg tagcagaagc tgagaaaaaa
     3781 gccaaggatc aaaaagaaga agatcgccgt aactacccaa ccaatactta caaaacgctt
     3841 gaccttgaaa ttgctgagtc cgatgtgaaa gttaaagaag cggagcttga actagtaaaa
     3901 gaggaagcta aggaatctcg aaacgaggaa aaagttaagc aagcaaaagc gaaagttgag
     3961 agtaaaaaag ctgaggctac aaggttagaa aacatcaaga cagatcgtaa aaaagcagaa
     4021 gaagctaaac gaagagcagc agaagaagat aaagttaaag aaaaaccagc tgaacaacca
     4081 caaccagcgc cggctcctca accagaaaaa ccaactgaag agcctgagaa tccagctcca
     4141 gctccaaaac ctgagaatcc agctgaacaa ccaaaagcag aaaaaccagc tgatcaacaa
     4201 gctgaagaag actatgctcg tagatcagaa gaagaatata atcgcttgac tcaacagcaa
     4261 ccgccaaaaa ctgaaaaacc agcacaacca tctactccaa aaacaggctg gaaacaagaa
     4321 aacggtatgt ggtacttcta caatactgat ggttcaatgg cgacaggatg gctccaaaac
     4381 aatggctcat ggtactacct aaacagcaat ggcgctatgg cgacaggatg gctccaaaac
     4441 aatggttcat ggtactatct aaacgctaat ggtgatatgg caacaggatg gctccaatac
     4501 aatgg
//
LOCUS       DBERDK010000103         4455 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.103, whole genome shotgun sequence.
ACCESSION   DBERDK010000103 DBERDK010000000
VERSION     DBERDK010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.103"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(56..1066)
                     /locus_tag="ACL73L_001962"
     CDS             complement(56..1066)
                     /locus_tag="ACL73L_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HGS0772870.1"
                     /translation="MSFLSKNGAGILACLLISILSWYLGGFFPVIGAPVFAIFIGMLL
                     HPFLSSYKQLDAGLTFSSKKLLQYAVVLLGFGLNISQVFAVGQSSLPVILSTISIALI
                     IAYLFQRFFALDTKLATLVGVGSSICGGSAIAATAPVIHAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSNEGFALFAGTAVNDTSSVTAAASAWDSLYQSNTLESATIVKLT
                     RTLAIIPITLFLSYWQSRQQENKQSLQLKKVFPLFILYFILASLLTTLLTSLGVSSSF
                     FTPLKQLSKFLIVMAMSAIGLKTNLVAMVKSSGKSILLGAICWIAIILTTLGMQTLIG
                     IF"
     gene            1215..2384
                     /locus_tag="ACL73L_001963"
     CDS             1215..2384
                     /locus_tag="ACL73L_001963"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HGS0772871.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQVISEIPGVLRLTLGEPDFTT
                     PDHVKEAAKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKAVILNYPANPTGITYSREQLEALAAVLRKYEIFVVCDEVYSELTYTGEAH
                     VSLGTMLRDQAIIINGLSKSHAMTGWRLGLIFAPATFTAQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGRYGEGYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            2381..3151
                     /gene="recO"
                     /locus_tag="ACL73L_001964"
     CDS             2381..3151
                     /gene="recO"
                     /locus_tag="ACL73L_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HGS0772872.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKRMFFVKHAGQS
                     KLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDAPLFAFLQKTLELMEAGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGACLCPEHYHEDKRRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            3148..4140
                     /gene="plsX"
                     /locus_tag="ACL73L_001965"
     CDS             3148..4140
                     /gene="plsX"
                     /locus_tag="ACL73L_001965"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HGS0772873.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIK
                     QYLTATERVSIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFIVGRIKNIDRPGLMSTLPTVDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLVADESLNFIGNVEARDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIMGLLKTAITGGGLRAKLGALLLKDSLRGLKKQLN
                     YSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETDVVAQTAREFSGE"
     gene            4146..4379
                     /locus_tag="ACL73L_001966"
     CDS             4146..4379
                     /locus_tag="ACL73L_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HGS0772874.1"
                     /translation="MTEKEIFDRIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQNVGDVVKIIQGK"
BASE COUNT         1297 a          840 c         1150 g         1168 t
ORIGIN      
        1 aacatatata cggcaaggcg acgttgacgt ggtttgaaga gattttcgaa gagtgttaga
       61 aaatgccgat aagggtctgc ataccaagag tggtgaggat gatggcaatc cagcagatgg
      121 ctccaagaag aatggatttt ccactggatt tgaccatagc gaccagattg gttttgagac
      181 cgatagcact catggccatg acaataagga atttagagag ttgtttgaga ggggtaaaga
      241 aactactgga cacacctaga gaggtgagta gtgtagtgag gagagaggca aggataaagt
      301 aaaggataaa aagtgggaag acttttttca gttgtaggct ttgcttgttt tcttgttggc
      361 gactttgcca gtaggataga aagagcgtga tagggataat ggccaaagta cgtgtgagtt
      421 taacaatggt tgcagactcg agggtattgc tttggtaaag actgtcccaa gcgctggcgg
      481 cagccgttac agaggaagtg tcgttgaccg cagtccctgc aaagagggcg aagccttcat
      541 tggatagatg aagccaggtg ccgagggttg gaaagatgag cgcagccaag acattgaaga
      601 aaaagataac ggaaatggct tgggctactt ccttttcctt agcatggata acaggcgctg
      661 tcgctgcaat ggcagaaccc ccacagatag aagaacctac tccaaccaag gtagccagtt
      721 ttgtatccag tgcaaagaaa cgctggaaga ggtaggcaat aatcagagct attgagatag
      781 tggacaggat gacagggagt gaagattggc caactgcgaa gacctgcgag atattgagac
      841 caaaaccaag caagacaacg gcatattgga gcaacttctt ggaactaaag gtcaaaccag
      901 catccagttg tttataggac gagagaaagg gatgtaggag catgcctatg aaaatggcaa
      961 aaacgggcgc gccaatcaca gggaagaatc ctcctaagta ccaagatagg atggaaatga
     1021 gaaggcaggc caagatacct gctccatttt ttgatagaaa tgacataaaa acctccaaat
     1081 aaaatctatt accattatag acctataaac aggaaaagta aaatagaaag tggaaagcta
     1141 ttctctaatg tatttttgcg gtcggggggc ttttgtagta taatagagat acgttttgaa
     1201 agtaggaggt atctatggac ttaactaagc gctttaataa acagttagat aaaattcaag
     1261 tttcgttgat tcgtcagttt gaccaggtta tttcggagat tcctggggtc ttgcgtttga
     1321 ccttggggga acctgatttt acaacgccag accatgtcaa ggaggcggcc aagcgagcga
     1381 ttgatcagaa ccaatcctac tatacaggga tgagtggtct gctgactcta cgtcaggcag
     1441 ccagtgactt tgttaaggaa aagtaccaac tggactatgc tcctgaaaat gaaatcttgg
     1501 ttacaattgg ggcgacagag gctttatctg cgactttgac ggctattttg gaagagggag
     1561 acaaggtact tttgccagct cctgcttatc caggctatga accgattgtt aacttagttg
     1621 gggcagaaat tgttgagatt gatacgactg aaaatggttt tgtcttgact cctgagatgt
     1681 tggagaaggc cattttggag cagggtgata agctcaaggc ggttattctc aactatccag
     1741 ccaatccgac aggaattacc tacagtcgag agcagttaga ggccttggca gctgttttac
     1801 gcaagtacga aatttttgtt gtctgtgatg aggtttactc agaattgacc tacacaggcg
     1861 aagcccatgt gtctctagga acgatgttga gagaccaggc tattattatc aatggtttgt
     1921 ctaaatcgca tgccatgaca ggttggcgtt tggggctgat tttcgctcct gcgaccttca
     1981 cagcccagtt aatcaagagt caccagtact tggtcactgc cgcaaatacc atggcgcaac
     2041 atgctgcggt agaagccttg acggctggta aaaacgatgc ggagcccatg aagaaggaat
     2101 atatccaacg tcgggactat atcatcgaaa aaatgactgc tcttggtttt gagattatca
     2161 aaccagacgg tgccttctat atttttgcta aaattccagc gggctacaat caagactcct
     2221 ttgcttttct gaaggatttt gctcagaaga aggccgttgc ctttatccct ggtgcagcct
     2281 ttggacgtta cggggaaggc tacgtccgcc tatcttatgc agccagcatg gagactatca
     2341 aagaagccat gaaacgactt gaggagtaca tgagagaagc atgattcagt ctatcacgag
     2401 tcaaggcttg gtgctttaca atcgcaattt tcgtgaggat gacaagctcg tcaaaatttt
     2461 tacagagcag gttggcaaac gcatgttttt tgtcaaacac gctggtcagt ctaagctggc
     2521 gcctgttatt cagcccttgg tgctggcacg atttctcttg cgaatcaatg atgacggact
     2581 cagttacatc gaagactatc atgaggtcat gacttttccc aagattaata gtgacctctt
     2641 tgtcatggcc tatgcgacct atgtggcagc tcttgcagat gctagtttgc aggacaatca
     2701 gcaggatgct cccttgtttg cttttttgca aaagactttg gagttgatgg aagcaggctt
     2761 ggattatcag gttttgacca atatttttga aattcaaatt ttgactcgat ttggaatcag
     2821 cctcaatttt aatgagtgtg tcttctgcca tcgggttggt caggcttttg acttttcttt
     2881 caaatatgga gcctgcctct gtccagagca ttatcatgag gataagagac gttgtcatct
     2941 caatcccaat atcccctatc tgctcaatca atttcaagct attgattttg agactttgga
     3001 gaccatttcg ctcaagcctg gaatcaagca agagctacgc caatttatgg atcaattata
     3061 tgaagagtac gttgggattc acctaaaatc aaagaaattt attgattccc tagcagactg
     3121 gggacaatta ctaaaagagg aaaagaaatg aaaaaaatcg cagtagatgc catggggggc
     3181 gattacgcac ctcaggccat tgttgagggt gtcaatcaag ccctatctga cttttcagat
     3241 atcgaggttc aactttacgg agatgaagct aaaatcaagc aatatctgac agcgacagag
     3301 cgcgtcagca ttatccatac ggatgagaag attgattcgg atgatgaacc tacgagagct
     3361 attaggaata agaaaaatgc cagtatggta ttggcagcca aggctgtcaa agatggtgaa
     3421 gcagacgctg tcctttcggc tgggaataca ggtgccttgt tggctgctgg attcttcatc
     3481 gtgggtcgta tcaagaatat cgaccgtcct gggcttatgt cgactttgcc gactgtagat
     3541 gggaagggat ttgacatgct agacctcggt gccaatgcgg aaaatacagc ccagcacctc
     3601 catcaatacg cggttctagg atccttctat gctaaaaatg ttcgtggcat tgcgcaacca
     3661 cgtgttggct tgctcaataa cggaacagag agtagcaagg gcgatccgct tcgtaaggaa
     3721 acttatgaat tactagtggc tgatgaaagt ttgaacttta tcggaaatgt ggaagcgcgt
     3781 gatttgatga atggcgttgc agatgttgtt gtggcagatg gtttcacggg aaacgctgtg
     3841 ctcaaatcca tcgaagggac agctatggga atcatgggct tgctcaagac agctattaca
     3901 ggtggtggtc ttcgagcgaa actaggtgcc ctccttctca aggacagcct cagaggtttg
     3961 aaaaaacagc tcaattattc agatgttggt ggagcggtct tgtttggtgt taaggcacct
     4021 gttgtcaaga ctcatggctc aagcgatgcc aaggctgttt atagtacgat tcgccagatt
     4081 cgtaccatgc tagaaacaga cgtagttgcc cagactgcgc gtgaattttc aggagaataa
     4141 aagagatgac agaaaaagaa atttttgacc gtattgtgac cattatccaa gagcgacagg
     4201 gagaggactt tgtcgtgaca gaatccttga gtctgaaaga cgatttggat gcggattctg
     4261 ttgacttgat ggagtttatc ttgactctgg aagatgaatt tagtatcgaa atcagcgatg
     4321 aagaaattga ccaactccaa aacgtaggag atgtggttaa aatcattcaa ggaaaatagc
     4381 aatcggagtt ccaagtcaac ggaagtagat ggtttttaga aatgagaaat atcggacaag
     4441 ctggtaaaat cttgg
//
LOCUS       DBERDK010000104         4418 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.104, whole genome shotgun sequence.
ACCESSION   DBERDK010000104 DBERDK010000000
VERSION     DBERDK010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.104"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(272..1498)
                     /locus_tag="ACL73L_001967"
     CDS             complement(272..1498)
                     /locus_tag="ACL73L_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGS0772875.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLA
                     ILLITISETLPQILQIFLGVLADFQHHRVLKYTVISFAKFLLYSIVSLSLSGQSFSLL
                     LVAFICLLNLLSDTLSYFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTINYQEGLSVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPVSTLFLRNQPFLNLQTGQSLALLSTFEL
                     SALIVGSLVSGYLQHTISIKTALYASLVIQLLLLVGFATVRFDWILIFSTLDAFFAGV
                     LSPRLQELVFKQIPEESMGAVQSSIGAITVVLPSLFTIALVTIATSFGTLAVSFVLLL
                     FLLVAFVMLLNIRESI"
     gene            complement(1570..2235)
                     /locus_tag="ACL73L_001968"
     CDS             complement(1570..2235)
                     /locus_tag="ACL73L_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HGS0772876.1"
                     /translation="MKKIISHRYFIIVFLLVIADQKFSVLVLRSDLVAGLSDFAYYLS
                     DMMLNFLVVLFALIAMIWSGKWQKINSRKFKGSYLFYSFLALLAFVAWNFVTFFLFPP
                     TRNEISYQHAAPTFTGATAFLMYFFYPVIAGPIFEDMIYRGLVMTALEKGKKWGLDVL
                     GSAVLFGVSHISNHGWVLTDFVFYMGGGLIFAVLFRMTKSIYWPIGLHIVYNGIGQLL
                     MLL"
     gene            complement(2303..2500)
                     /locus_tag="ACL73L_001969"
     CDS             complement(2303..2500)
                     /locus_tag="ACL73L_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone VP1"
                     /protein_id="HGS0772877.1"
                     /translation="MLNLQFAETMELTEAELETVYGGEFGNNAVIPAGAWGGLGTSWS
                     ITNFWKKYFNHDSSTVNRRHY"
     gene            complement(2791..3654)
                     /locus_tag="ACL73L_001970"
     CDS             complement(2791..3654)
                     /locus_tag="ACL73L_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HGS0772878.1"
                     /translation="MIERMELGEFYKELRLARKLKQTDVACEGLTASQLSKFELGQSM
                     LSADKLILAIQGINVTFDEFGHKLNNYQESPHMRIGRKVVNRFAHQDIAALEQLLEEV
                     DQEQMAQTYRRLNAIVIKDAIHSLNKSYPLAEEDSEFLTTYLYAIESWTWFELYLFCN
                     TMPFLSNQDLIFLSTSLLEKSKEFKELVHNRLYMKQGLLNILSELMERKLFSYIPIFE
                     AELERMLRPYDVFEKVSWQFLKKMSVFLQTKGSNQKEIERFIQSLQVLENPQLTSLFE
                     LRFQQYKELID"
     gene            complement(4049..>4418)
                     /locus_tag="ACL73L_001971"
     CDS             complement(4049..>4418)
                     /locus_tag="ACL73L_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HGS0772879.1"
                     /translation="IEINAPVIEAIDISNTNRKQFIVKQILERKPKIVGIYKLGMKYN
                     SDNYKESAILSIINELLIVGIKILVYEPNLNVSIDNVIIEKNFELFTKQSDLIVANRW
                     DRGLEAYKDKVYTRGIWIRD"
BASE COUNT         1530 a          830 c          758 g         1300 t
ORIGIN      
        1 gacgtggttt gaagagagtt tcgaagagta taagtaggct gaaaagaatc cagccacagc
       61 atggactatt atatagcaga ttgaaataag atgagaacaa atcgattggg aaagtaaaat
      121 taatttctat aaatgtttta gcaattgttt cgtactattt tagattcagt ctattataac
      181 acattcagaa aagagaaaaa agtctgttga ttttgaccat cataaaaaga ctggcaatcc
      241 agtctcaaac atatattata gaaattctcc actaaatact ttcacgaata ttcagaagca
      301 taacaaaggc aactagaaga aatagcaata aaacaaagct aactgccaga gttccaaagc
      361 tagtagcaat ggttaccaaa gctattgtaa ataagctagg taaaacaacc gtaatggcac
      421 cgatagagga ttgaactgct cccattgact cctcaggtat ttgtttaaaa acgagttctt
      481 gcaatctagg agagagaaca cctgcgaaaa aggcatccaa ggtactaaag atgagaatcc
      541 agtcaaaacg aactgtggca aatcctacta gaagaagcaa ctggatgaca agtgaggcat
      601 agagagctgt ttttatggaa atggtatgtt gcagatagcc acttacaagg cttccgacaa
      661 tcagggctga cagttcaaaa gtggatagca aggcaagaga ttgacctgtt tgtaaattca
      721 aaaagggttg attcctcaaa aatagagtag agacaggaac tgtaacattt atcactgctt
      781 gactagtaga gataataaac aaaaccaaga gcaccttatt catattccat atcaatttcg
      841 atgattggag caaatgctgg caaaaggatt ttacagagag tccttcttga tagttaatcg
      901 ttttttctac tttcaagagg tcagttttta tgaagaggat acctaaaaat gcgattaaaa
      961 aggtaagagc gttcagcaag gaaatgaact ggatggagag aatccctagc aagactcctc
     1021 ctagaatatt actgattgtt ttcactaaac taacagttga ctgtttaaat ccaatagctt
     1081 ctgccagatg gtcttgccca ataattctaa tgaaaatcgg agtgagcatg gctcctgaaa
     1141 agtaactcaa tgtgtcagac aagaggttaa gcagacaaat aaatgctact agcaacaagg
     1201 agaaagactg ccctgaaagt gataaagaaa cgatagagta aagcaaaaat tttgcaaaac
     1261 taatgactgt gtatttcaag acacgatgat gttgaaaatc tgccaaaact cccagaaaga
     1321 tttgtaaaat ttggggaagg gtttctgaaa tcgtaatgag taaaatcgcc aaaggtgcaa
     1381 aagacgcatc tgccacataa ttcagaaagg ccagataaaa aatcgtatcc ccaagcgttg
     1441 aaatccactg gttgatagtt aattgcctaa aatctctatt ttgaagaaat actttcatca
     1501 caactccttc ttaagttcaa atgggaatct ttccccaagg atagaccgcg atactactaa
     1561 caaccaaaat tacagtaaca tcaaaagctg accaatgcca ttgtagacta tatgcagtcc
     1621 aataggccaa taaattgact ttgtcattct aaataagact gcaaatataa gacctccacc
     1681 catatagaag acaaagtctg tcaagaccca accgtgatta ctaatgtgcg agaccccaaa
     1741 taaaacagcg gaaccaagta catctagccc ccatttcttt cctttttcca gagcagtcat
     1801 cactaatcca cgataaatca tgtcttcaaa aatgggacct gcaatcacag gataaaaaaa
     1861 atacatcaaa aatgccgtag ctcctgtaaa agtaggagca gcatgttgat aagaaatttc
     1921 atttcgagta ggtgggaaaa gaaaaaaggt gacaaaattc caagcaacaa aagcaagcag
     1981 agctaggaag gaatagaaaa gataggatcc tttaaacttt ctactattga ttttctgcca
     2041 tttccccgac caaatcatag caataagagc aaataaaacc acaagaaaat tcaacatcat
     2101 atccgacaga taataggcaa agtcagatag cccagcaaca aggtcgctgc gtaaaactaa
     2161 aacactgaac ttctggtcag caataactag tagaaaaact ataataaagt agcggtgtga
     2221 gattatcttt ttcattctat tttctcctat actatgaaat aaagacagtc cctacccaag
     2281 aaaaaggcaa ggactctgaa tatcaataat ggcgacgatt aacagtggaa gaatcatggt
     2341 taaaatattt cttccagaaa ttagtgattg accaagacgt tcctaaacct ccccaagcac
     2401 cggctgggat aacagcatta ttcccaaatt cccctccata aactgtctcc aactcagctt
     2461 ctgtcaattc cattgtttct gcaaattgta aatttaacat cttttacact ccttcaatta
     2521 tcttcatttg taaaccactt ctgcgaccta ggatttgctt caagtgcttt acaagtacag
     2581 tataacacga aaattggctt attttaaaaa atcgcatatt tgatattttt tcttatagaa
     2641 atttcttatt tgcgattttg atagatttga ttatttccct ggtataataa agttattact
     2701 aacaaggagg aatataatag atgaagaaaa gaaaaatcca actgatactc ctacttattt
     2761 cggagtgggt tattgtcatt ccatttttaa ctaatctata agttctttat attgctgaaa
     2821 acgcaattca aaaagggatg ttaattgtgg attttctaat acctgcagag attgaataaa
     2881 gcgttcaatc tctttttgat tgcttccctt tgtttgaaga aagacactca tcttctttaa
     2941 aaattgccac gatacttttt caaaaacatc atacggtcgt aacatcctct ccaactcggc
     3001 ttcgaagatt gggatgtagg agaaaagttt tcgctccatg agttctgata agatatttaa
     3061 gagtccttgc ttcatataca atcgattgtg tactaactct ttaaattctt tggatttttc
     3121 gagtaaggag gttgataaaa aaatcagatc ttgattgctc aagaagggca tggtattgca
     3181 aaagagatag agttcaaacc aggtccaaga ctcgatagca tagagatagg tggtcaaaaa
     3241 ctcgctatcc tcctctgcta gtgggtagct tttatttagt gaatggatgg catctttaat
     3301 cacgatggca ttcaaacgac gataggtctg cgccatctgt tcttgatcga cttcctccaa
     3361 tagctgctct aaagcagcta tatcctgatg ggcaaagcga ttcacaacct ttcgaccgat
     3421 tcgcatatgt ggagattctt gatagttgtt gagcttgtgc ccaaactcat caaaggtcac
     3481 atttatacct tggatagcta gaatcaactt atccgcagac agcatagact gccctagttc
     3541 aaacttggac aactgagaag ctgttagacc ctcacaagcc acatctgtct gcttaagctt
     3601 tctcgccaaa cgtaattcct tgtaaaattc ccccagttcc attctctcaa tcatctgacc
     3661 acctcctagc ttttgcatat tatatcatta tttacaatca tttgtcaaaa tattctgata
     3721 tttaagaggg caaaaaagcc agccttaagc tgactctaaa aggaccgagc cttgtacaat
     3781 acagaactca atcctcgttc taaatagaat gtctaacttt tttgggcagt acacgagctt
     3841 ggggaacagc cactggaacg atgaggtgtg agctcaaaat atcctccagt tatgtttttc
     3901 ctaatagtat accggaagag tgaaaggatt ttataatgga gcggttacaa agaacctact
     3961 ttctattaaa cagtatacta tgaaaatgtg aaaatttaac atttttttgt acaaatttta
     4021 taaattattg cctttttaat atcaatagtt aatctcttat ccagatcccc cttgtgtaaa
     4081 ctttatcttt ataagcttca aggcccctat cccatctatt tgcaacaatt agatcacttt
     4141 gttttgtaaa tagttcaaaa ttcttttcaa taattacgtt atctatacta acgtttaaat
     4201 ttggttcata tactaaaatt tttataccga caatcaatag ttcattaatt atacttaaaa
     4261 tagctgactc tttgtaatta tctgaattat atttcatccc caatttatat attcctacta
     4321 tctttggctt tcgttccaat atttgtttaa ctatgaactg ttttctattt gtgtttgaaa
     4381 tatcaatcgc ttctatcact ggggcattta tttctata
//
LOCUS       DBERDK010000105         4382 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.105, whole genome shotgun sequence.
ACCESSION   DBERDK010000105 DBERDK010000000
VERSION     DBERDK010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4382
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.105"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            123..1457
                     /gene="pepC"
                     /locus_tag="ACL73L_001972"
     CDS             123..1457
                     /gene="pepC"
                     /locus_tag="ACL73L_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HGS0772880.1"
                     /translation="MNAIQESFTDKLFANYEANVKYQAIENAASHNGIFAALERRQSH
                     VDNTPVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAILNKLLRQDAQILRDLLVSGADQATVQAKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSTKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAEPIVLAPWDPMGALAE"
     gene            complement(1513..2424)
                     /locus_tag="ACL73L_001973"
     CDS             complement(1513..2424)
                     /locus_tag="ACL73L_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HGS0772881.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(2448..3251)
                     /locus_tag="ACL73L_001974"
     CDS             complement(2448..3251)
                     /locus_tag="ACL73L_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HGS0772882.1"
                     /translation="MSIISMVLVVVVAFFAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAAIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPAALL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            complement(3279..4268)
                     /locus_tag="ACL73L_001975"
     CDS             complement(3279..4268)
                     /locus_tag="ACL73L_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGS0772883.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQTAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNVKANVVPIQKLIEISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
BASE COUNT         1333 a          922 c          934 g         1193 t
ORIGIN      
        1 agagattttc gaagagtata aattcgaaaa aggtgaggga caatgtcctc gccttttatg
       61 ttttttagtt gcttcctttg tgaaaagagt tataatagac tgtagaataa aaggaggaat
      121 ctatgaacgc gattcaagaa tcatttactg ataaactatt tgccaactat gaagcaaatg
      181 taaaatacca agcgattgaa aatgctgcca gccacaacgg aatttttgca gctctggaac
      241 gtcgccaaag ccatgtagac aacacacctg ttttctcatt ggatttaacc aaggacaagg
      301 tcactaacca gaaagcgtct ggtcgttgct ggatgtttgc ggctctcaac accttccgcc
      361 acaaactcat ctcgcaatac aaattggaga actttgagtt gtcacaagcc cacactttct
      421 tctgggacaa gtatgagaaa tcaaactggt tcttggagca agtcattgcg acttcagacc
      481 aagaattgac tagccgcaag gttagcttct tactccaaac acctcaacaa gatggcggtc
      541 aatgggatat ggtcgtttcc ctctttgaaa aatacggtgt cgtgcctaag tcagtttatc
      601 ctgagtctgt ttcatctagc agcagtcgtg agctaaatgc gatccttaat aaattgcttc
      661 gtcaagatgc tcaaatcttg cgtgatttgc ttgtttctgg tgcagatcaa gcgactgttc
      721 aagctaagaa agaagacctc ttgcaagaaa tctttaactt tcttgctatg tcattaggac
      781 ttccaccacg caagtttgac tttgcttatc gcgataaaga taacaactac aaaagtgaaa
      841 aaggaatcac accacaagag ttttacaaga aatatgtcaa tcttccttta gaagactacg
      901 tttctgttat caatgctcca actgctgata aaccttacgg aaaatcttac acagttgaga
      961 tgttggggaa tgtggttggt agccgtgcag ttcgctacat caacgttcca atggagcgct
     1021 tgaaagaatt ggcgattgcc caaatgcaag caggtgagac tgtttggttt ggttctgatg
     1081 tcggccagct cagcaaccgt aaggctggca tccttgcgac agatgtttat gactttgaat
     1141 caagcatgga cattaaactt actcaagaca aggctggacg tttggactat agtgaaagct
     1201 tgatgaccca cgccatggtc ttgacaggtg ttgacttgga cgaaaatggt aaatcaacca
     1261 agtggaaggt tgaaaactca tggggagaca aggttggtac agatggttac tttgttgcct
     1321 cagacgcttg gatggacgaa tacacatacc aaatcgttgt tcgtaaggaa ttgctgacag
     1381 cagaagaaca agctgcctat ggagcagaac caatcgtact tgcaccatgg gatccaatgg
     1441 gagccttggc tgaataaaag catagaaaaa aggaatcaga ttttagaacc tggttccttt
     1501 ttagttgctt gattacatga tgtgaagaac atgtgccaca atacccactg cgaagagggc
     1561 aaggataata gtgattggag atactttttt cttaagtaag tacatgcaaa gtaaagtaag
     1621 gagtagtcct gataatccag gaatcaacat atccaagttt tgttggaaag tagtaacttt
     1681 ttcaggagtt tgagacaatc cttgtcctac ttgtgcgaat gcttcttgga tacctttaga
     1741 cccttctggc aatttatccc aatggatata agccttttca tctagttgaa ctttagaaac
     1801 atcgaaagca aatttaatat ttacccaacg ttgaacaagg acagcaagaa tgaacatccc
     1861 aaggatagaa gctcctttag tgatatcttg aaggatacca ccagacatat ctttagtgat
     1921 ttctgatcca gccttgtatc caatctcttg aacataccac aagaatgaca tacgaatcaa
     1981 gttccatgca acaaagaaga ggagtggccc caagatattg ccagtaaggg caagtgaagc
     2041 accgagagat ccaaggattg ggcgtactgt aaaccagaat actgggtcac cgataccagc
     2101 aagaggtccc atcataccga ttttaacccc ttggatagca gcgtcatcga tttccacacc
     2161 gttagcacgt tcttcttcaa gcgcaagagt aacccccatg actggagcag ctacgtatgg
     2221 atgagtgttg aagaactcaa ggtgacgctc aagagcagcg atttgatctt cttttttagt
     2281 atagagtttt ttgatagctg gaatgagtgt ataagcccag cccaagtttt gcatccgttc
     2341 aaagttccaa gacccttgta agaaggttga acgccaccaa acttttttac gatctgattt
     2401 agttaattga agtttttcag tcatgatgtt ttcagtcctt tcttatctta gtagtcttct
     2461 aggatatcgc cgattgggtc gttagaagtt gcggctcctc cgccaccatt tccaccagtt
     2521 ttagaaaggt gaaggtagat aagagcgata gcaacaccga tagcaccgaa tccgattaga
     2581 gtaatatctg acacagcagc gagaacgaaa ccaagagcga agaatggcca tacttcacga
     2641 gttgccatca tgttgataac catggcgtaa ccaacggcaa cgaccatacc accaccgata
     2701 gccataccat ctttgagcca gtctggcatg gcactaagga tactttgtac agtttcagtt
     2761 ggtaccataa ggagaagagc tgcaggaagc gcgatacgaa gtccttggaa aagtagcgca
     2821 atgaaatgag cacgctccac agcgccgaag tcaccttttt tagcggcagc atcagcagtg
     2881 tgaaccaatc caactgaaat tgtacgaaca atcatagtta agaatagacc agctacggca
     2941 agagggatag caaccgcttg ggcaacaccg ataccagtct tggtaaagtc accaccaaga
     3001 accatgataa tggcagcagc gacagaagca agtgcagcat caggagcgat agcagcaccg
     3061 atatttgacc aaccaagggc aatcatttga agcgatccac cgaggataat ccctgcttcc
     3121 aagtgacctg ttacaagccc aataagggta caggctacaa gtggttggtg aaattggaac
     3181 tggtcgagga tgccttcaag acctgcaaag aaggctacaa cgactactaa aaccatagaa
     3241 ataatagaca tgtttaaaat cctttcataa ataatggctt atttgacatt ggctttgtta
     3301 atcaagtcaa acaaatcttt tttagaatca tttggtactt tacggacatc aaattcaaca
     3361 cccaagtcac gcattttttc aaatgtagca acatcttctt tgtccataga caaaacggta
     3421 ttgaccaatg ttttacctgt tgagtgagcc atagaaccaa cgttaagagt cttgattggc
     3481 acgccgcctt cgatggcacg aagggcatct tgaggtgttt caaacaagat aagggcatgt
     3541 gtttctccaa aacgtgggtc ttttgaaatc tcaatcagtt tttgaattgg aaccacgtta
     3601 gccttgacat tacctggagc tgcttgttta atcaattctt tacgaaggtc gtctttagcc
     3661 acgttatctg aagcaacgat gatacgattt gcttttgaat ctggagtcca agcagttgca
     3721 acctgaccgt gaagtagacg tgtgtcaaga cgggcaagat tgattttcaa tttaccgtct
     3781 ccgataacag ttccttctgg gatagcagtt tgggcaactg gagcagctgc agcgcttgca
     3841 acttcttcaa ctggatttag ctcttctgga agagctttga tgccatcttt ggcttcttta
     3901 atgatattag cagcgacttt ttctacacct gcagcagcgt ccatgaggcg ctctgtgtag
     3961 gcttgaatca acatcggtaa gttaagtcct gtgatgatgg caaacttacg ctcaggattt
     4021 tctcccatca cgcgactagc ttggttaaat ggagaaccac tccaaaggtc agccaaaact
     4081 agaacctcat cttctgcgtc aaatgcagca acagcgttat taaacttagc gtatagatca
     4141 tcaggacctt catttggcat aaaggtcaca acttgaacct tttcttgttc accaaagatc
     4201 atagatcctg actgatgaat acccgcagca aattcgccgt ggctcgcaat aatgattccg
     4261 atactcatta ttgtcattcc tccttttttg ttttagtttt catttaagaa aactttaaga
     4321 ctgtttaagt ataaaccgtt ttcataaaaa aagcaactat ttatcataaa aaattaaaaa
     4381 at
//
LOCUS       DBERDK010000106         4108 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.106, whole genome shotgun sequence.
ACCESSION   DBERDK010000106 DBERDK010000000
VERSION     DBERDK010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4108
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.106"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            74..319
                     /locus_tag="ACL73L_001976"
     CDS             74..319
                     /locus_tag="ACL73L_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772884.1"
                     /translation="MKAFYITIEKFIRDDESLKCYFIKVISSRSKVTSLDQIEADKTI
                     QRKYSSELKKFIGFYNEIICEENSFLHVRKRWSSWFR"
     gene            720..1712
                     /locus_tag="ACL73L_001977"
     CDS             720..1712
                     /locus_tag="ACL73L_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGS0772885.1"
                     /translation="MAMIEVEHLQKNFVKTVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQDNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPNSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQEIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYEGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
     gene            1714..2532
                     /locus_tag="ACL73L_001978"
     CDS             1714..2532
                     /locus_tag="ACL73L_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS0772886.1"
                     /translation="MIKLWRRYKPFINAGVQELITYRVNFILYRIGDVMGAFVAFYLW
                     KAVFDSSQESLIQGFSMADITLYIIMSFVTNLLTRSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTELGSKWLIFISVGLPFLSVIVLMKIISGQGIVEVLGLTVLYLFSLTLA
                     YLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAFMSGSLIPLAFFPKVVSDILSFLPF
                     SSLIYTPVMIIVGKYDASQILQALLLQFFWLLVMVGLSQLIWKRVQSFITIQGG"
     gene            2534..3319
                     /locus_tag="ACL73L_001979"
     CDS             2534..3319
                     /locus_tag="ACL73L_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS0772887.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLAQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLMLISLVFFVISLKLWDKGLDSYESAGS"
     gene            3358..3636
                     /locus_tag="ACL73L_001980"
     CDS             3358..3636
                     /locus_tag="ACL73L_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HGS0772888.1"
                     /translation="MFVIEEVKDENQKKAVVAEVLKDLPEWFGIPESTQAYIEGTTTL
                     QVWTAYQESDLTGFVSLSYSSEDCAEIDCLGVKKLIKVEKLGANCLLL"
     gene            3621..3818
                     /locus_tag="ACL73L_001981"
     CDS             3621..3818
                     /locus_tag="ACL73L_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS0772889.1"
                     /translation="MLATLESEARKKVGYLQVKTVAEGSNKDYDRTNDFYRGLGFKKL
                     EIFLQLWNPQNPCQILIKKLE"
BASE COUNT         1147 a          697 c          888 g         1376 t
ORIGIN      
        1 gctatatatt atggtatcga atcttcatca gaatgataaa attaatcaat tgatatctga
       61 ttacaaacag aatatgaaag ctttttatat cactattgaa aaatttatac gagatgatga
      121 aagccttaag tgttatttta taaaggttat ttcaagtcgt tccaaggtaa caagtctaga
      181 tcagattgaa gctgataaaa cgatacaaag aaaatattca agtgagctaa aaaaatttat
      241 tggattttat aatgagatta tttgtgagga aaatagtttc ctacatgtac gaaagaggtg
      301 gtcgagttgg tttaggtagt cgatgcgtga gttgataatt ctcagggtat ggacttcttt
      361 ttcatgaatg aggtaaaaga gcaggtattg tttagagaca atcattctga gcatattttc
      421 tggatagagg gagtatccga ttttatgatc aaagttaata ccgccctctg gtgagaagat
      481 aagtaggttg gtaatttaaa ctattaaaca gaatttttga ttaaaagtat tattttatga
      541 gagaaatcct aatttcacaa tccataggca aacgcttgca tttcgttttt tattggacta
      601 taataggttg gtataaagcc ttttgtagta ataaaatgta gaaggtgtag aaagcaagga
      661 tttagaatat ttgtagttaa aaacacaatg ttgctattcc ttacgatagg gagatagata
      721 tggcaatgat agaagtggaa catcttcaga aaaattttgt gaagactgtt aaggaaccgg
      781 gcttgaaggg ggctttgcgc tcctttattc atcctgaaaa gcagaccttt gaagcggtca
      841 aggatttgac ctttgaggtt ccaaaagggc agattttagg atttatcggg gcaaatggtg
      901 ctgggaagtc gacaaccatt aaaatgctga caggaatttt gaaaccaaca tctggttttt
      961 gtcggattaa cggcaagatt ccccaggaca atcggcaaga ttatgtcaaa gatattggcg
     1021 tagtctttgg acaacgcacc cagctatggt gggatttggc tctgcaagag acctacactg
     1081 tcttaaaaga gatttatgat gtgccaaact cgctctttca taagcgcatg gactttttga
     1141 atgaagtctt ggatttgaag gactttatca aggatcccgt gcggactctt tcactgggac
     1201 aacggatgcg ggcggatatt gcggcctcct tgctccacaa tcccaaggtt ctttttttag
     1261 atgagccgac cattggtttg gacgtttcgg ttaaggataa tattcgtcgg gcaattactc
     1321 agatcaatca agaggaagaa actaccattc ttttgaccac tcacgatttg agtgatattg
     1381 agcaactttg tgatcggatt ttcatgattg acaaggggca agagattttt gatggaacgg
     1441 tgagccaact caaggagacc tttggtaaga tgaagactct ctcttttgaa ctgctaccag
     1501 gtcaaagtca tctcgtctct cactatgaag gtctgtctga tatgaccatt gatagacaag
     1561 gaaacagcct caacattgaa tttgatagtt ctcgctacca gtcagctgac attatcaagc
     1621 aaaccctgtc tgattttgaa atccgcgatt tgaagatggt ggatacggat attgaggata
     1681 ttatccgtcg cttctaccga aaggagctct aggatgatca aattgtggag acgttataaa
     1741 ccctttatca atgcaggggt tcaggagttg attacttacc gagtcaactt tattctctat
     1801 cggattggcg atgtcatggg ggcttttgtg gccttttatc tctggaaggc tgtctttgat
     1861 tcttcgcaag agtctttgat tcagggcttc agtatggcgg atatcaccct ctacatcatc
     1921 atgagttttg tgaccaatct tctgactaga tccgattcgt cctttatgat tggggaggag
     1981 gtcaaggatg gctccattat catgcgtttg ttgcgaccag tgcattttgc ggcctcctat
     2041 cttttcaccg agcttggttc caagtggttg atttttatca gcgttggcct tccattttta
     2101 agtgtcattg tcttgatgaa aatcatatcg ggtcaaggta ttgtagaggt gctaggatta
     2161 actgtccttt atctttttag cttaacgctc gcctatctga ttaacttttt ctttaatatt
     2221 tgctttggat tttcagcctt tgtgtttaaa aatctttggg gttccaacct acttaagact
     2281 tccatagtgg cttttatgtc ggggagtttg attcccttgg cattctttcc aaaggttgtt
     2341 tcagatattc tttccttttt gcctttttca tccttgattt atactccagt tatgatcatt
     2401 gttggaaaat acgatgccag tcagattctt caggcactcc ttttgcagtt cttctggctc
     2461 ttagtgatgg tgggattgtc tcagttgatt tggaaacggg tccagtcctt tatcaccatt
     2521 caaggaggtt agtatgaaaa aatatcaacg aatgcatctg atttttatca gacaatacat
     2581 caaacaaatc atggaatata aggtggattt tgtggttggt gtcttgggag tctttctggc
     2641 tcaaggcttg aatctcttgt ttctcaatgt catctttcaa catattccat tcctagaagg
     2701 ctggaccttt caagagatag ctttcattta tggattttcc ttgattccca agggaatgga
     2761 ccatctcttt tttgacaatc tctgggcact agggcaacgc ctagtccgaa aaggagagtt
     2821 tgacaagtat ctgactcgtc ccatcaatcc tctctttcac atcctagttg aaacctttca
     2881 gattgatgcc ttgggtgaac tcttagtcgg tggtatttta ttgggaacaa cagtgaccag
     2941 cattgtttgg actcttccaa aattcctgct tttcctagtt tgtattcctt ttgcgacctt
     3001 gatttatact tctcttaaaa tcgcaacagc cagtatcgcc ttttggacta agcagtcagg
     3061 cgccatgatt tacatcttct atatgttcaa tgactttgct aagtatccga tttctattta
     3121 caattctctt cttcgttggt tgattagctt tatcgtgcct ttcgccttta cagcctatta
     3181 tccagctagc tatttcttac aggaaaagga tgtgttcttt aacgtaggag gtttgatgtt
     3241 gatttctctg gttttctttg ttatttccct taaactttgg gataagggct tagattccta
     3301 cgaaagtgcg ggttcgtaaa agctaaagta agactaaaat caagaaagaa acttatgatg
     3361 tttgtaattg aagaagtcaa ggatgaaaat caaaaaaagg cagttgtagc tgaggttttg
     3421 aaggatttgc cagaatggtt tggaatccca gaaagcacac aagcctatat agaaggaacc
     3481 acgacactgc aagtttggac cgcctatcag gagagtgatt tgactggatt tgtaagctta
     3541 tcctattcga gtgaagattg tgcagagatt gattgtctcg gcgtaaaaaa gcttatcaag
     3601 gtagaaaaat tgggagccaa ttgcttgcta ctttagagag tgaagctcgt aaaaaagttg
     3661 gttatctgca ggtcaaaaca gtggcagaag gttctaataa agattatgat cgaacaaatg
     3721 acttttatcg aggtcttggc tttaaaaagt tagagatttt tcttcaacta tggaatccgc
     3781 aaaatccttg tcagattttg attaaaaagc ttgaataata ttacttgaca tctattctca
     3841 gagtgctata ctgtaagtgt aatcgccgat ttagcttagt tggtagagca aggcactcgt
     3901 aaagcctagg ttataggtag ataaacgact gaggatttga aaaaatagat aggtagaaga
     3961 taaccgttaa gccttattct tagcggttat ttatattgtt taatagcgct gatatttcat
     4021 caattatgcc tgttttcgtg tttctggtag ttgttcaagt ttattgctac tatttttgat
     4081 ggtatgaatg tgcttataat gtatccct
//
LOCUS       DBERDK010000107         3880 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.107, whole genome shotgun sequence.
ACCESSION   DBERDK010000107 DBERDK010000000
VERSION     DBERDK010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3880
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.107"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            331..1677
                     /gene="gdhA"
                     /locus_tag="ACL73L_001982"
     CDS             331..1677
                     /gene="gdhA"
                     /locus_tag="ACL73L_001982"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HGS0772890.1"
                     /translation="MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVVSFRVPWVDRDGKIQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKEKRRARLTEYAAEKATATYHGGSVWTYAGNYDIALPCATQNEINGEAAKR
                     LVDQGVICVSEGANMPSDLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDGRLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGIV"
     gene            complement(2183..2353)
                     /locus_tag="ACL73L_001983"
     CDS             complement(2183..2353)
                     /locus_tag="ACL73L_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772891.1"
                     /translation="MKKENEYVILTIASLGVMIGIVFAIFLDFPVEYGISLGLLNGIV
                     LGSLIVYKNNKN"
     gene            complement(2366..2938)
                     /locus_tag="ACL73L_001984"
                     /pseudo
     CDS             complement(2366..2938)
                     /locus_tag="ACL73L_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082046.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(2961..3766)
                     /locus_tag="ACL73L_001985"
                     /pseudo
     CDS             complement(2961..3766)
                     /locus_tag="ACL73L_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT         1192 a          734 c          653 g         1301 t
ORIGIN      
        1 tataatgctt tttgatttta gtctggtata aatattgctt tcctccaaaa tggtcatagt
       61 tttactggca aatctaacat atcacggata aattaacaag tgatttctga attactaaac
      121 attttctttt cttatagcat accatacttt aagattttgt ctttaagaaa gatatttcca
      181 agaaaaacgt tcgttttttt atctattttc aaaaaaacca aaaactgaat tgaaagaatt
      241 tttagaaaat ttctgttttt tctttacagt gaaaaaaaat tctgctataa tgtcttatgt
      301 aataaaatat gacaataaaa ggagaacgat atgacatctg ctaaagaata tatccaaagc
      361 gtgtttgaaa ctgtgaaagc tcgtaacggg cacgaggctg aattcctcca agctgttgaa
      421 gaatttttca acactttaga acctgtattt gaaaaacacc ctgagtatat cgaagaaaat
      481 atcttggcac gtattactga gcctgagcgc gtggtttctt tccgtgttcc ttgggttgac
      541 cgtgatggaa aaattcaagt aaaccgtggt taccgtgttc aattcaactc agctgttgga
      601 ccatacaaag gtggacttcg tttccaccca actgtaaacc aagggatctt gaaattcctc
      661 ggatttgaac aaatctttaa aaatgtcttg actggacttc ctatcggtgg aggtaaaggt
      721 ggatcagact tcgatcctaa aggtaaaaca gatgctgaag tgatgcgctt ctgccaaagc
      781 ttcatgactg aattgcaaaa acacatcgga ccatcacttg acgtacctgc tggtgatatc
      841 ggtgttggtg gacgtgagat tggttacctt tacggtcaat acaaacgtct taaccaattt
      901 gatgctggtg tcttgactgg taaacctctt ggatttggtg gtagcttgat tcgtccagaa
      961 gcaactggtt acggtttggt ttactatact gaagaaatgc tcaaagctaa cggtaacagc
     1021 tttgctggta agaaagtcgt tatttcaggt tctggtaacg ttgctcaata tgctcttcaa
     1081 aaagcaactg aactcggtgc aactgttatt tctgtttctg actcaaatgg ttatgtcatc
     1141 gatgaaaatg gtatcgactt cgatcttttg gttgatgtta aagaaaaacg tcgtgctcgt
     1201 ttgactgagt atgcagctga aaaagcaact gctacttatc atggaggttc tgtatggact
     1261 tacgctggta actatgatat cgctcttcca tgtgcgactc aaaacgaaat caacggtgaa
     1321 gcagctaaac gtttggttga tcaaggcgtt atctgtgtat ctgaaggtgc caacatgccg
     1381 agcgaccttg atgccatcaa agtttacaaa gaaaatggta tcttctacgg acctgccaaa
     1441 gctgccaacg ctggtggtgt agccgtttca gcccttgaaa tgagccaaaa tagtcttcgc
     1501 ctctcatgga ctcgtgaaga agttgatggc cgtctcaaag acatcatgac aaacatcttc
     1561 aacacagcta aaacgacttc agaaacatac ggtcttgata aagactacct tgcaggagca
     1621 aacattgctg cctttgaaaa tgtagcaaat gccatgattg cacaaggtat tgtttaagat
     1681 ttatttactc aatcctcaat cactacgatt ggggattttt agcttgaatt aaaaaattgg
     1741 aaaatctaat taaataaaat gaaatgaaat aaaaactgaa caaattgatt gcggaattca
     1801 aaccaatttc taacatactt ctagaaatta atttgacttt tctaatcgat ttgttcatat
     1861 cttatttcaa tttactatac ttacttaacc ctttttatac tcaatgaaaa tcaaagagca
     1921 aactaggaag cgagccgcag gctgtacttg agtacggcaa ggcaaagctg acgtggtttg
     1981 aagagatttt caaagagtat taaacaaaaa gaaaaaccgc tactcctaag aatagcaatt
     2041 taatcatgtt tttaagctac tactgtagtt gctctattat ttaagtgtta tgaataactt
     2101 caatatcttt aaagcttttt gttagattta taatttcaac tatagccatc tctactcctt
     2161 agtttacagc aaaaaattat gcttaattct tattgttttt gtaaacaatc agcgaaccca
     2221 atactattcc attcaacaag cctaaagaaa taccatattc aactggaaaa tctaaaaaaa
     2281 ttgcaaacac tattccaatc atcaccccta gtgaggctat tgttaaaatt acatattcat
     2341 tttctttctt cattttcttt acacctcatt tcctttttgt atataagctg tagtactctc
     2401 caccaagagt tattaagtac ttatgatttc ctgactcaac aatcttacca cctttcatta
     2461 caaaaataat atccgcatcc ttaatcgttg acaatctatg agctataatt atttgagtac
     2521 aaccctgact ttgtatatac tttgttattc tttcctcatt aatagtgtct aatgcactag
     2581 ttgcttcatc taaaattaca atactaggat tatttattaa tgcacgtgcc agtgctatcc
     2641 gttgcctttg cccacctgaa atatttgacc ccatctctga gatgatagta ttaaatttca
     2701 tcggcatagc catgatttca tcatagattt gaactgcttt acaaacttcc tctatctttt
     2761 gtgaagtaac ttcgtgcttt aaagttatat tatcaagaat acttcggttc aataaaaatg
     2821 aatcctgtgg aactactcct aaattttgac ttaatatttt tttatctatt tgattaatat
     2881 ttataccata aaataaaact tttcctgtat caatcttata taatcctgat ggtatttttg
     2941 ataatagact tcctgcaaaa ctagaatcct agttcatgat tgataatacc aacaatcaaa
     3001 ttcatccgta atccgaagcg tttacgatga tttcgatagg ttgttgaaaa cattttaagc
     3061 gtttttactt tggcaaagat gttctcaacc ttgcttctct ccttagatag cacatggtta
     3121 caggctttat cttcagctgt tagcggcttg agtttgctgg atttacgtgg agtttgtgct
     3181 tgaagatata tcttcatgag cccttgataa ccactgtcag ccaagatttt accagcttgt
     3241 ccgatatttc tgcgactcat tttgaacaac ttcatatcat gacaatagtt cacagcgata
     3301 tccaaagaaa taattctccc ttgacttgtg acaatcgctt gagtcttcat agcgtgaaat
     3361 ttctttttac cagaataatt cgctaattct ttttttaggt cgattgattt ttacttccgt
     3421 cgcatcaatc attaccgtgt cctcagaact gagaggagtt cttgaaatcg caacaccacc
     3481 tttgaacaag agttacttca acccattggc tccgacggat taagttgctt tcgtgaatac
     3541 caagatcagc cgaaatttct tcataagttc gatattctcg cacatattga agagtggcca
     3601 taagaaggtc ttctaggctt aatttaggtt ttcgcccacc ttttgcgtgt ttaagttgat
     3661 aagctgtttt taatacagct aacatctctt caaaagtcgt gcgttgaaca ccaacaagac
     3721 gcttaaatcg tgcatcggtt agttgtttac ttgcttcata attcatagaa ctattatacc
     3781 atattttgtt tcgcaggaag tctattgaaa aatctccaga ctagagaact cacggatagt
     3841 tcctaatctg gagatttctt atttgcactt ttcttgtaca
//
LOCUS       DBERDK010000108         3533 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.108, whole genome shotgun sequence.
ACCESSION   DBERDK010000108 DBERDK010000000
VERSION     DBERDK010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.108"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..300
                     /locus_tag="ACL73L_001986"
     CDS             <1..300
                     /locus_tag="ACL73L_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772892.1"
                     /translation="QNRLHNIYKGFTHYKYYRAENSHLIYADYFEMKLKKLLKDDTKV
                     FEKSTFKFVEGYKIYLTESKESGIKQMDNVIKYFEFIESKSIALYFQKRLNELID"
     gene            complement(654..1187)
                     /locus_tag="ACL73L_001987"
     CDS             complement(654..1187)
                     /locus_tag="ACL73L_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HGS0772893.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENAIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
     gene            complement(1276..2052)
                     /gene="recX"
                     /locus_tag="ACL73L_001988"
     CDS             complement(1276..2052)
                     /gene="recX"
                     /locus_tag="ACL73L_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HGS0772894.1"
                     /translation="MKITKLEKKKRLYLMELDNGDKCYITEDTIVRFMLSRDKVISEE
                     ELKEIQDFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDINENIVSQVIANLKEEKW
                     INDSQYAYAIINANQLSGDKGPYVLTQKLAQKGISKSTIEEILNDFDFSEVAQRVANK
                     LLKKYEGKLPARALQDKIIQNLTNKGFSYFDAKIAFDELDSQVDEETTQELIFKELDK
                     QYTKYARKYEGYELKQRLTQVLARKGYDFSDIASALREYL"
     gene            2090..3457
                     /gene="rlmD"
                     /locus_tag="ACL73L_001989"
     CDS             2090..3457
                     /gene="rlmD"
                     /locus_tag="ACL73L_001989"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HGS0772895.1"
                     /translation="MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYC
                     QITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQ
                     ALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
                     DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNR
                     QLNLTQLVKELVKDFPEVVTVAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSL
                     SPRAFYQLNPEQTEVLYNEAVKALDVNKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDI
                     IPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLL
                     DTILTYVPEKMVYISCNVSTLARDLVRLVEVYDIPYIQSVDMFPHTARTEAVVKLIKK
                     IRKSS"
BASE COUNT         1173 a          599 c          619 g         1142 t
ORIGIN      
        1 caaaataggc tccataatat ctataaggga tttacccact acaaatatta tagagccgaa
       61 aattcacatc taatatatgc agactacttt gaaatgaaat taaaaaaatt attaaaggat
      121 gacacaaaag tttttgaaaa atctacattc aaatttgtag aaggatataa aatatacctg
      181 acagaatcta aagaatctgg aattaaacaa atggacaatg tcataaaata ttttgagttt
      241 attgaatcta aaagtattgc tttatatttt caaaaacgat taaatgagct gatagattaa
      301 atagcatttt ctctgttgag atattgtttt taaaatattg tactaaatga ttgatgctat
      361 gtggaaatac aaaaaaatgt ttttgatacg aagttgacct gtatttttta tactaatcat
      421 tttcgtattt tttgtattaa acgatataag tttgttgtaa acttacaagg aataaagaca
      481 ttaaaaaata acagtatatc tatttgtttt atatatttta cgaattctgc ataaatctct
      541 ttctagtaat gtgttgtaac tctgctataa tagatttatt cctttttgtg tttacacaat
      601 ttattttata ctaccaaaaa aggtcaggat tttgttcctg acctttgaca actttaccga
      661 ttctttagtt ctacatagcg cttgtaccaa atgtttacat aggcttctga gaaaggacca
      721 cgtccattgt taatccaatc aacaagaatt ttgacatgtt cttttaaaat atagtccaag
      781 tcatcagaat aattcatttt gcgtttgtga cgctcgtact cttcaacgtc caagagacgt
      841 ttttccccat ctgtaaaaat tttaacatcc aaatcgtaat caatatactt cagtgcttct
      901 tcatccagat agtaggggct agccatattg caatagtaag aagttccatt atcacgaatc
      961 atggcaatga tattaaacca atatttcttg tgaaagtaaa caatagccgg ttctcgagtg
     1021 acccaacgac gaccatcact ttcggtaaca agtgtatgat cgttgacacc aataatggcg
     1081 ttttctgttg tttttagtac catggtgtcc cgccaagttc ggtggagact cccatcatgc
     1141 ttataacttt gaattgtaat aaagtcgcct tcttttggaa gcttcataac taaccaactt
     1201 tctacaattt ataagtttat catttactat tgtaccataa aattacccaa aatctgtgaa
     1261 tttcacttgg aaatattaaa gatattctct aagagcgctt gctatatccg aaaaatcgta
     1321 gccctttcgt gctaaaactt gagttaaacg ctgcttcagt tcgtatcctt catactttcg
     1381 ggcatactta gtatattgct tatcaagttc cttgaagatg agttcctgag tcgtttcttc
     1441 atcaacttga ctatccaatt cgtcaaaggc aattttagca tcaaaataag agaagccctt
     1501 gttagtcaag ttctggataa tcttatcttg cagggcacga gctggaagtt ttccctcata
     1561 ttttttcaat agtttattgg ctacacgttg agcaacttcc gaaaaatcaa aatcattcaa
     1621 gatttcttct atagtagatt ttgaaattcc tttttgtgct aatttctgag tcagtacata
     1681 aggtcccttg tctcctgaaa gttgattggc attgatgata gcataagcgt actggctatc
     1741 attaatccac ttctcttctt taagattagc aatgacttga gaaacgatgt tttcattaat
     1801 atcatatttt ttcagatatt ctctgacctc tttttcagta cgtgctttaa aggataagtg
     1861 gtagagggcc agattcttac cataagaaaa ttgagcaaag tcttgaatct ctttcaattc
     1921 ctcttcgctt atcaccttat ctctcgataa cataaaacga acaattgtgt cttcggtgat
     1981 atagcatttg tcgccattat caagctccat cagatagagt ctttttttct tttcaagttt
     2041 tgtgattttc atagctctat tataactcaa aatgtgataa gataggggta tgaatctgaa
     2101 agtgaaacaa aaaataccat taaaaatcaa gcgcatggga attaacggtg agggaatcgg
     2161 cttttaccaa aaaacattag tctttgtacc aggagctctc aaaggcgaag atatctattg
     2221 tcagattact tctattagac gcaactttgt tgaagcaaaa ttactgaagg tcaacaagaa
     2281 gtctaaattt cgaattgtgc catcttgtac tatttataat gaatgcggag gctgccaaat
     2341 catgcacctg cattatgata agcagctgga gttcaagacg gacttacttc atcaagcgct
     2401 gaaaaaattt gctcctgcag gatatgaaaa ttatgaaatt cgtccaacta ttggaatgca
     2461 ggaaccaaaa tattacagag ctaagttaca atttcagact cgaaaattta aaaatcaggt
     2521 caaggcgggc ttatatgcac aaaactctca ctatttagta gagttgaaag actgcctggt
     2581 acaagataag gaaacccaag tgattgctaa tcgcttagca gaattactta cttatcacca
     2641 gattccaatc acggatgaga gaaaagttct aggtgtccgt actattatgg tccgacgcgc
     2701 gagaaagacc ggacaggttc agattattat tgttacaaac cgccagctta atttaactca
     2761 attggtaaaa gagttggtta aagatttccc agaagttgtg acagtagctg ttaatacaaa
     2821 tacagctaaa accagtgaga tatatggtga aaagacagag attatctggg ggcaagagag
     2881 tattcaagaa ggtgtactca attatgaatt ttcactatcc cctcgagctt tttatcaact
     2941 aaatcctgaa caaacagaag ttctttataa cgaggcggta aaagccttgg atgttaataa
     3001 agaagaccat ttgattgacg cttattgtgg agttggaacg attggttttg cctttgcaaa
     3061 gaaagttaaa acacttagag gtatggatat tattccagaa gctattgaag atgctaagcg
     3121 aaatgctaaa agaatgggat ttgacaacac acattacgaa gcaggaacag cggaagagat
     3181 tattcctcgc tggtataagg aaggctaccg agcagatgct ctgattgtgg acccaccacg
     3241 tacaggtctg gatgataagt tattagatac tattcttacc tatgtaccag aaaaaatggt
     3301 ctatatttct tgcaatgttt caactttggc aagggatttg gtaaggttag tagaagtcta
     3361 tgatatccct tatattcagt cggtcgatat gtttccacac actgctcgaa ctgaagctgt
     3421 tgtaaaatta attaaaaaaa ttagaaaaag tagttgacaa aacataaaaa ggctgtataa
     3481 tagtaagagt tgaaaataac aactcaggtc cgttggtcaa ggggttaaga cac
//
LOCUS       DBERDK010000109         3532 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.109, whole genome shotgun sequence.
ACCESSION   DBERDK010000109 DBERDK010000000
VERSION     DBERDK010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.109"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            387..3506
                     /gene="phtE"
                     /locus_tag="ACL73L_001990"
     CDS             387..3506
                     /gene="phtE"
                     /locus_tag="ACL73L_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HGS0772896.1"
                     /translation="MKFSKKYIAAGSAVIVSLSLCAYALNQHRSQENKDNNRVSYVDG
                     SQSSQKSENLTPDQVSQKEGIQAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDALFSE
                     ELLMKDPNYQLKDADIVNEVKGGYIIKVDGKYYVYLKDAAHADNVRTKDEINRQKQEH
                     VKDNEKVNSNVAVARSQGRYTTNDGYVFNPADIIEDTGNAYIVPHGGHYHYIPKSDLS
                     ASELAAAKAHLAGKNMQPSQLSYSSTASDNNTQSVAKGSTSKPANKSENLQSLLKELY
                     DSPSAQRYSESDGLVFDPAKIISRTPNGVAIPHGDHYHFIPYSKLSALEEKIARMVPI
                     SGTGSTVSTNAKPNEVVSSLGSLSSNPSSLTTSKELSSASDGYIFNPKDIVEETATAY
                     IVRHGDHFHYIPKSNQIGQPTLPNNSLATPSPSLPINPGTSHEKHEEDGYGFDANRII
                     AEDESGFVMSHGDHNHYFFKKDLTEEQIKAAQKHLEEVKTSHNGLDSLSSHEQDYPSN
                     AKEMKDLDKKIEEKIAGIMKQYGVKRESIVVNKEKNAIIYPHGDHHHADPIDEHKPVG
                     IGHSHSNYELFKPEEGVAKKEGNKVYTGEELTNVVNLLKNSTFNNQNFTLANGQKRVS
                     FSFPPELEKKLGINMLVKLITPDGKVLEKVSGKVFGEGVGNIANFELDQPYLPGQTFK
                     YTIASKDYPEVSYDGTFTVPTSLAYKMASQTIFYPFHAGDTYLRVNPQFAVPKGTDAL
                     VRVFDEFHGNAYLENNYKVGEIKLPIPKLNQGTTRTAGNKIPVTFMANAYLDNQSTYI
                     VEVPILEKENQTDKPSILPQFKRNKAQENSKLDEKVEEPKTSEKVEKEKLSETGNSTS
                     NSTLEEVPTVDPVQEKVAKFAESYGMKLENVLFNMDGTIELYLPSGEVIKKNMADFTG
                     EAPQGNGENKPSENGKVSTGTVENQPTENKPADSLPEAPNKKPVKPENSTDNGMLNPE
                     GNVGSDPMLDPALEEAPAVDPVQEKLEKFTASYGLGLDSVIFNMDGTIELRLPSGEVI
                     KKNLSDLIA"
BASE COUNT         1314 a          575 c          689 g          954 t
ORIGIN      
        1 gcggaggcct tgctagaaaa agtaacagat tctagtatta gacaaaatgc tatggagaca
       61 ttgactggtc taaaaagtag tcttcttctc ggaacgaaag ataataacac tatttcagca
      121 gaagtagata gtctcttggc tttgttaaaa aaaagtcaac cggctccgat acagtagtaa
      181 aatgaatgga gcatatttta tggagaagta acctttcgtg ttacttctct tttttagaaa
      241 aacgtaacag aaacttgaca aaagtaattt taaatagtaa actatttact ggttaattaa
      301 atggttaaat aaccggttta gaaaactatt taataaagta aaagaagttg agaaaaaact
      361 tcatcattta ttgaaatgag ggatttatga aatttagtaa aaaatatata gcagctggat
      421 cagctgttat cgtatccttg agtctatgtg cctatgcact aaaccagcat cgttcgcagg
      481 aaaataagga caataatcgt gtctcttatg tggatggcag ccagtcaagt cagaaaagtg
      541 aaaacttgac accagaccag gttagccaga aagaaggaat tcaggctgag caaattgtaa
      601 tcaaaattac agatcagggc tatgtaacgt cacacggtga ccactatcat tactataatg
      661 ggaaagttcc ttatgatgcc ctctttagtg aagaactctt gatgaaggat ccaaactatc
      721 aacttaaaga cgctgatatt gtcaatgaag tcaagggtgg ttatatcatc aaggtcgatg
      781 gaaaatatta tgtctacctg aaagatgcag ctcatgctga taatgttcga actaaagatg
      841 aaatcaatcg tcaaaaacaa gaacatgtca aagataatga gaaggttaac tctaatgttg
      901 ctgtagcaag gtctcaggga cgatatacga caaatgatgg ttatgtcttt aatccagctg
      961 atattatcga agatacgggt aatgcttata tcgttcctca tggaggtcac tatcactaca
     1021 ttcccaaaag cgatttatct gctagtgaat tagcagcagc taaagcacat ctggctggaa
     1081 aaaatatgca accgagtcag ttaagctatt cttcaacagc tagtgacaat aacacgcaat
     1141 ctgtagcaaa aggatcaact agcaagccag caaataaatc tgaaaatctc cagagtcttt
     1201 tgaaggaact ctatgattca cctagcgccc aacgttacag tgaatcagat ggcctggtct
     1261 ttgaccctgc taagattatc agtcgtacac caaatggagt tgcgattccg catggcgacc
     1321 attaccactt tattccttac agcaagcttt ctgccttaga agaaaagatt gccagaatgg
     1381 tgcctatcag tggaactggt tctacagttt ctacaaatgc aaaacctaat gaagtagtgt
     1441 ctagtctagg cagtctttca agcaatcctt cttctttaac gacaagtaag gagctctctt
     1501 cagcatctga tggttatatt tttaatccaa aagatatcgt tgaagaaacg gctacagctt
     1561 atattgtaag acatggtgat catttccatt acattccaaa atcaaatcaa attgggcaac
     1621 cgactcttcc aaacaatagt ctagcaacac cttctccatc tcttccaatc aatccaggaa
     1681 cttcacatga gaaacatgaa gaagatggat acggatttga tgctaatcgt attatcgctg
     1741 aagatgaatc aggttttgtc atgagtcacg gagaccacaa tcattatttc ttcaagaagg
     1801 acttgacaga agagcaaatt aaggctgcgc aaaaacattt agaggaagtt aaaactagtc
     1861 ataatggatt agattctttg tcatctcatg aacaggatta tccaagtaat gccaaagaaa
     1921 tgaaagattt agataaaaaa atcgaagaaa aaattgctgg cattatgaaa caatatggtg
     1981 tcaaacgtga aagtattgtc gtgaataaag aaaaaaatgc gattatttat ccgcatggag
     2041 atcaccatca tgcagatccg attgatgaac ataaaccggt tggaattggt cattctcaca
     2101 gtaactatga actgtttaaa cccgaagaag gagttgctaa aaaagaaggg aataaagttt
     2161 atactggaga agaattaacg aatgttgtta atttgttaaa aaatagtacg tttaataatc
     2221 aaaactttac tctagccaat ggtcaaaaac gcgtttcttt tagttttccg cctgaattgg
     2281 agaaaaaatt aggtatcaat atgctagtaa aattaataac accagatgga aaagtattgg
     2341 agaaagtatc tggtaaagta tttggagaag gagtagggaa tattgcaaac tttgaattag
     2401 atcaacctta tttaccagga caaacattta agtatactat cgcttcaaaa gattatccag
     2461 aagtaagtta tgatggtaca tttacagttc caacctcttt agcttacaaa atggccagtc
     2521 aaacgatttt ctatcctttc catgcagggg atacttattt aagagtgaac cctcaatttg
     2581 cagtgcctaa aggaactgat gctttagtca gagtgtttga tgaatttcat ggaaatgctt
     2641 atttagaaaa taactataaa gttggtgaaa ttaaattacc gattccgaaa ttaaaccaag
     2701 gaacaaccag aacggccgga aataaaattc ctgtaacctt catggcaaat gcttatttgg
     2761 acaatcaatc gacttatatt gtggaagtac ctatcttgga aaaagaaaat caaactgata
     2821 aaccaagtat tctaccacaa tttaaaagga ataaagcaca agaaaactca aaacttgatg
     2881 aaaaggtaga agaaccaaag actagtgaga aggtagaaaa agaaaaactt tctgaaactg
     2941 ggaatagtac tagtaattca acgttagaag aagttcctac agtggatcct gtacaagaaa
     3001 aagtagcaaa atttgctgaa agttatggga tgaagctaga aaatgtcttg tttaatatgg
     3061 acggaacaat tgaattatat ttaccatcgg gagaagtcat taaaaagaat atggcagatt
     3121 ttacaggaga agcacctcaa ggaaatggtg aaaataaacc atctgaaaat ggaaaagtat
     3181 ctactggaac agttgagaac caaccaacag aaaataaacc agcagattct ttaccagagg
     3241 caccaaacaa aaaacctgta aaaccagaaa actcaacgga taatggaatg ttgaatccag
     3301 aagggaatgt ggggagtgac cctatgttag atccagcatt agaggaagct ccagcagtag
     3361 atcctgtaca agaaaaatta gaaaaattta cagctagtta tggattaggt ttagatagtg
     3421 ttatattcaa tatggatgga acgattgaat taagattgcc aagtggagaa gtgataaaaa
     3481 agaatttatc tgatctcata gcgtaaggaa tagcagtaga aaaagtctga at
//
LOCUS       DBERDK010000110         3447 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.110, whole genome shotgun sequence.
ACCESSION   DBERDK010000110 DBERDK010000000
VERSION     DBERDK010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.110"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(170..424)
                     /locus_tag="ACL73L_001991"
     CDS             complement(170..424)
                     /locus_tag="ACL73L_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HGS0772897.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLILAYFSVRDITLMPVKKSKRR"
     gene            complement(444..2285)
                     /gene="typA"
                     /locus_tag="ACL73L_001992"
     CDS             complement(444..2285)
                     /gene="typA"
                     /locus_tag="ACL73L_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HGS0772898.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPAEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSR
                     KVEERLQAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IVKEIDGVKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMS
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            2447..3172
                     /locus_tag="ACL73L_001993"
     CDS             2447..3172
                     /locus_tag="ACL73L_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HGS0772899.1"
                     /translation="MRLDNLLAQEKVSRKAMKQALLRGEILVDGCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLD
                     RDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVQVNGLLTPDHIQAFQKGIVFLDGTVC
                     KPAKLEILSASPSLSQASITISEGKFHQIKKMFLSVGVKVTSLKRIQFGDFTLNPDLA
                     EGNYRPLNQKELQIIKNYLEMSR"
     gene            complement(3212..3439)
                     /locus_tag="ACL73L_001994"
     CDS             complement(3212..3439)
                     /locus_tag="ACL73L_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772900.1"
                     /translation="MKEKKKNPLFVGILSIILGLLFPIVGLILGIIGLVLAISYQKES
                     QLDYKIEKILNILGIVISVVNWIVAIALIFR"
BASE COUNT         1034 a          756 c          643 g         1014 t
ORIGIN      
        1 tcaaaataat atgcaaccag tactaaccaa atataaaata gatgccatta acgaatttta
       61 ttcaagtttt tcccattcat actatacaag caaaagagat ggtgttaact aaaaagcaat
      121 tcaaactatt ataaaattcc tagcaaaaag agagccgaaa ctctcttttt tatcttcttt
      181 tacttttttt gactggcatg agtgtgatgt ctctaacact aaagtaagct aggatcaaca
      241 tggctattgc taggaatatt tctgttggta attgaaaaat tttcagaaaa gatagaacca
      301 ataaaatcaa gagtgccact aaaatacata ccatagcgac gatattgact gtccctttaa
      361 tgctttctgg tgtcgcaaat acatagagta ggagcagtaa aattcctagg actaaataga
      421 ccatctttct ctctttctag ctcttattca gctgattttt tcttcttatt agctttctca
      481 cgctctgctt tgttaaggat ttgtttacgc aaacggatag actcaggcgt tacttccatg
      541 tactcatcgt cgttcaagaa ctcaagagac tcttcaagtg tcaagatacg aggcgtcttg
      601 ataacagctg tttggtcctt agtagctgaa cgaacgttgg tcatttgttt tgccttcgtg
      661 atgttaactg tcaagtcatt ttcacgagag ttttcgccga tgatcattcc ttcataaacc
      721 tcagtacctg ggttgacaaa gatcgtacca cgttcttcga tagacatgat tgagtaagtt
      781 gtagccttac cagcatcgat agaaacaagg gcaccacggt gacgtccacc aatttcccct
      841 ggaatcaatg gcaagtattg gtcgaaggta tggttcatga taccgtaacc acgagtcatt
      901 gacaagaact cagttgagta tccaatcaaa ccacgcgctg gaacaaggaa gaccaaacga
      961 gtttgaccat taccagttga aatcatatcc aacatttcac ctttacgttc agaaaggctt
     1021 tggataacag acccttggta ttcttctgga gtgtcgattt gtacacgttc aaatggttca
     1081 catttaacac cgtcgatttc ttttacgata acttctggac gagatacttg aagttcatag
     1141 ccctcacgac gcattgtttc gataaggatt gacaagtgca attctccacg tcctgaaaca
     1201 gtccatttat ctggtgaatc agttgggtca acacgaaggg aaacgtctgt ttgcaattct
     1261 gcctgcaagc gttcttccac cttacgagaa gttacccatt taccttcttt accagcaaat
     1321 ggtgagttgt tgaccaagaa agtcatttga agagttggct catcgatgtg taggattgga
     1381 agagcttcta ctgcatctgt cggagtgatg gtttcaccga caaagatgtc ttccatacct
     1441 gaaacggcaa tcaagtcacc cgctttggct tcttggattt cacgacgttc caaaccaaag
     1501 aaaccgaaga gttttgtaac acggaagttt ttagttgtac cgtcaagttt agaaagggta
     1561 acttggtccc caaccttaac tgtaccacgg aagacacgac cgataccgat acgtccaacg
     1621 aagtcattgt agtccaaaag tgacacttgg aactgcaaag gctcatctga gttatctact
     1681 ggagctggga tatggtcgat aatcgtgtca aagattggtg ccatagtcgc ttcttggtca
     1741 gctggatcat ctgacaatga agaagttccg ttgatcgctg aagcataaac cactgggaaa
     1801 tcaagctggt cgtcatctgc accaagctcg atgaaaagtt ccaagacttc atccactact
     1861 tctgctggac gagctgatgg cttatcgatt ttgttaacaa ccacgattgg gacaaggtct
     1921 tgttccaagg cttttttcaa tacgaaacga gtttgtggca tggttccttc ataggcatct
     1981 acgaccaaga caacaccgtc aaccattttc atgatacgct caacttctcc accaaagtcc
     2041 gcgtgtcctg gtgtgtccat aatgttgata cgagttccgt tgtaagcaac ggcagtattt
     2101 ttagcaagga tggtgattcc acgctctttt tcgatatcgt ttgagtccat agcacgctct
     2161 gccaattcag tacgtgcatc aagcgtttct gattgtttca ataattcgtc aaccagggtt
     2221 gttttaccgt ggtcaacgtg ggcgataatc gcaatgttac ggatatcttc tcttaatttt
     2281 gtcatgattt cctctataat attcaaaatt tattttctaa ctgaacgatt ataccataat
     2341 ttcaaataaa taacataact caagcaagtg taaatgtttt cactccgctt ttcttttcac
     2401 gtcaagcctt ttcaaagcaa gcgacttatg ataagatagg cacagtatgc gtttagataa
     2461 tttattagcc caagaaaaag ttagccgaaa ggccatgaag caagcactcc tcagagggga
     2521 aattctagtc gatggttgcc cagcccgatc cctagctcaa aatatcgata caggactaca
     2581 agaactcctt tttcaggatc gaatcattca aggctatgaa cacacctatc ttatgcttca
     2641 taaacctgct ggtgtcgtta cagccaacaa agacaaggaa cttccgaccg tcatggacct
     2701 gcttccatct gacatccagt ctgacaagct ctatgccgtt ggccgactgg accgagatac
     2761 aacgggactc ctcctcttga ccgataacgg tcccttgggc tttcagctcc tccatcccca
     2821 atatcatgtc gataagactt accaagtcca ggttaatgga cttctaacac ctgaccatat
     2881 ccaagccttt caaaagggaa ttgtcttttt agatggtact gtctgtaaac ccgcaaaact
     2941 agagattcta tctgcaagtc cctccctcag tcaagcctct atcaccattt cagaaggaaa
     3001 atttcatcaa atcaagaaaa tgttcctctc ggttggtgtt aaggtgacta gcctcaaaag
     3061 aatccaattt ggggacttca cattgaaccc agatttagca gaaggtaact accgcccttt
     3121 gaaccaaaaa gagttacaaa tcattaaaaa ctatttagag atgagtcgat aaaacaaaaa
     3181 aagctttaaa actaaagctt ttttctttta tttaccgaaa aattaaggcg attgctacaa
     3241 tccagttaac tacagaaatc acaattccta agatattaag aatcttttct attttatagt
     3301 ctaattgtga ctctttttgg tatgaaatag ccaagaccaa tcctatgata cccaaaatca
     3361 ggcctacaat tggaaataac aaaccaagaa taatcgacaa gatacccaca aaaagtggat
     3421 ttttcttctt ttctttcatg ttctaag
//
LOCUS       DBERDK010000111         3149 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.111, whole genome shotgun sequence.
ACCESSION   DBERDK010000111 DBERDK010000000
VERSION     DBERDK010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.111"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     regulatory      complement(95..183)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(392..1021)
                     /gene="thiE"
                     /locus_tag="ACL73L_001995"
     CDS             complement(392..1021)
                     /gene="thiE"
                     /locus_tag="ACL73L_001995"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HGS0772901.1"
                     /translation="MFHKELLKLYFICGTTTCQGKNLYTVVEEALRGGITLFQFREKG
                     EGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIKNEEEFQQSKVEYVDYVGVGPVFDTQSKDDAGGAIGYEGVKLMRKLL
                     PQMPLVAIGGIQTKHIKDIMKTNVDGVSIISAISYAKNIEKTVREMSEQ"
     gene            complement(1023..1805)
                     /gene="thiM"
                     /locus_tag="ACL73L_001996"
     CDS             complement(1023..1805)
                     /gene="thiM"
                     /locus_tag="ACL73L_001996"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HGS0772902.1"
                     /translation="MTSLKLLKEKAPLVICITNDVVKNFTANGLVALGASPAMSEFPA
                     DLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPVVLDPVACGAGEYRKKVA
                     DDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
                     GEVDAIAVNGEVVTIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVY
                     NIAGEMAEKRPNGHLPGTFKVEFINALYEITDEDVKEFKRVK"
     gene            1907..2089
                     /locus_tag="ACL73L_001997"
     CDS             1907..2089
                     /locus_tag="ACL73L_001997"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772903.1"
                     /translation="MKIKEQTRKLTADCSKHCFEVVDRTDEVSNHTYGKVKLTWFEEI
                     FEESYLIKFVNIMSLL"
     gene            complement(2086..2754)
                     /locus_tag="ACL73L_001998"
     CDS             complement(2086..2754)
                     /locus_tag="ACL73L_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HGS0772904.1"
                     /translation="METQDYAFQPGLTVGELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKE
                     LKVAENDYLEVTLHPVTKAFQDLMYWAVASSDYAHLLVMLVIAEGLYLDWGSKDLALP
                     EVYIHSEWINLHRGPFFAEWVQFLVDELNRVGKNREDLTELQQRWNQAVALELAFFDI
                     GYDV"
     regulatory      complement(2969..3057)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT          985 a          682 c          441 g         1041 t
ORIGIN      
        1 catcttcatc cagtttcgta aaagaaaaaa ctctaattac agatacgaat tagagttcag
       61 cttacaagat tagacagttc ttttcgacat acgaaaaaaa catttcacat ttcccttcgc
      121 cagtcttaac tgtatcaggt tcaatgggta tcatctcagc ctaaagcacc ccaaatgcct
      181 ttattattta attatgtgat tattataaca cacattttat actagttcaa gaaattgaac
      241 tggaaataca gccttgcact cacaaagaca gcagatcttt cttttgcaaa aaacaaatga
      301 cctgtttgat gaattagcta ttcaagctga atctagacat agttttttaa aaaaggaaaa
      361 tcctacttac ttagaatcca aggatagata tctattgttc actcatttcc cgaacagttt
      421 tttctatatt ttttgcatac gatattgccg aaatgattga aacgccgtca acattggtct
      481 tcataatgtc tttaatatgt ttcgtctgta tcccaccaat tgcaactaag ggcatttgtg
      541 gcaatagttt tctcatcaat ttaacacctt cataacctat agcaccacca gcatcatcct
      601 ttgactgggt atcaaataca ggaccaacac caacataatc tacatattca acttttgatt
      661 gttgaaattc ttcctcgttt tttatagaaa gaccaattat tttatctggc atcaattttc
      721 taatttcatc aacaccaata tcatcttgac ctacatgtac gccatcagcg tcaatttcca
      781 ttgccaaatc gatatcatca ttaacgataa acggcacatt gtatttttta caaagttctt
      841 taatttggat agctagctca agtttttcta agccttctaa agcaccctca cctttttctc
      901 gaaattgaaa taaggttata ccacctctta aggcttcctc aacgactgta tatagatttt
      961 ttccttggca agtagtcgtt ccacaaataa aatatagttt tagtaattct ttatgaaaca
     1021 tcttacttca ctcttttgaa ttcctttaca tcttcatctg taatctcgta taaggcattt
     1081 ataaattcaa ctttaaatgt cccaggaaga tgtccatttg gacgtttttc tgccatttct
     1141 ccagcgatat tgtaaaccaa cattgctgtt tctaatgatt tcaattcttg acctttttct
     1201 agtccgataa agcttgctac cactgcccct aataaacatc cagttccaat aacttttggc
     1261 atcatggcac taccattatg aatcgttacc acttctccat ttaccgcaat agcatccact
     1321 tcacctgtta ctactattgg aatattgaac ttctcatttg ctgctagagc aatctcgtca
     1381 atattatcta ctcccgcact atctactcct ttagatgcca catctattcc tactaaagag
     1441 gcaatctctc cagcattccc cctaatcgca gctagtttgt aattgttgat taaatcatct
     1501 gctacttttt ttctatattc tcctgctcca caggctacag gatctaaaac tactgggaca
     1561 ttatatttct ctgcaatttt cagagcagct tggtataatt tccaattttc atctgtcaat
     1621 gttcctatgt ttattaataa accaccagca tactttaaca aatcctctaa atctgctgga
     1681 aactcactca tggctggtga ggcgcccagt gctactaatc catttgctgt gaaatttttt
     1741 actacatcat tggtgataca aatgaccaat ggtgcttttt cttttaataa ttttaaactt
     1801 gtcatattga aatccttcct tttcacttta tacgatctac tgatttcgat ttatttctat
     1861 ctttcgttaa gaattttttt catttacatt gaatgattta tactcaatga aaatcaaaga
     1921 gcaaactagg aagctaaccg cagattgctc aaaacactgt tttgaggttg tggatagaac
     1981 tgacgaagtc agtaaccata cctacggcaa ggtgaaactg acgtggtttg aagagatttt
     2041 cgaagagtct tacctcatca aatttgtaaa tatcatgagc cttctctaga catcgtaacc
     2101 aatatcaaaa aaagctaatt ctaaagcgac tgcttgattc catcgttgct gaagttctgt
     2161 caaatcttct cgatttttac cgacacgatt gagttcgtca accagaaatt gaacccactc
     2221 tgcaaagaaa ggacctctgt ggagattgat ccattccgaa tgaatataga cttcaggtag
     2281 agccaaatct ttagaacccc agtctaaata gagaccttct gcaatgacca gcataaccaa
     2341 aagatgggca tagtctgatg aagccactgc ccaatacatt agatcctgaa aggcttttgt
     2401 tacagggtgc aaagtcactt ctagatagtc attctctgct acttttaact ctttaaaagc
     2461 cttttggaaa taaccatctt catctgcttc aagaaagcct agttgcttgg caaaacgaag
     2521 cttggattca agtttatctg cgtgagctac gcaggcaccc agcatggata agaaggcatc
     2581 aaagaagtga taatcttgaa tcagatagtc ctttaagacc ttattctcaa ttgtccccgc
     2641 aaaaagttcc ttaacaaaac gatgattgat tgcagcctgc caatccttct gactgctttt
     2701 taataattct ccaacagtca aacctggctg aaatgcatag tcttgtgttt ccatatttac
     2761 ttctcctctc tttacttgtt agtaattaat aaaacaccaa gaaatatcaa gcaaaatcgt
     2821 aattccactt gatcctttta aaacacatcg agagtatttg cagagagcta actaaacaag
     2881 cctatccagt ttatataaac aaaaaactcc aattacaatc aagaattaga gttgacttac
     2941 aagattagac cgttcatttc accatacgaa aaaactgttc acatttccct tcgccagtct
     3001 taactgtatc aggttcaatg ggtattatct cagcctaaag caccccaaat gtctctatta
     3061 tttaattact gaaccagtat agcaaaaaat gaaagcccta gcaagatatt tgaccgaaaa
     3121 atatctttat atataatata ttgaaactg
//
LOCUS       DBERDK010000112         3072 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.112, whole genome shotgun sequence.
ACCESSION   DBERDK010000112 DBERDK010000000
VERSION     DBERDK010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.112"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..812
                     /locus_tag="ACL73L_001999"
     CDS             <1..812
                     /locus_tag="ACL73L_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HGS0772905.1"
                     /translation="AILAGSSLAISGVLMQSVTRNPIADPGILGINTGASLSVVIGLS
                     FLGISSSISHISFAIIGGLVSAIFVYAIAVSGKAGLTPIKLALSGTCVSMALSSFVSF
                     LILPNNNVLDKFRFWQIGSLGAATLSSISTLLPFIILGHLIAIFISSDLNALAMGDEM
                     AVGLGVNVNRIRSLAIIASVLLCSSITAIGGPIGFVGLIVPHFCGLFISKDIRTMTIS
                     SSFIGAELLLICDIIGRMLGKPGEIEVGIITAIIGGPVLIYVTMKNRGGNN"
     gene            812..1819
                     /locus_tag="ACL73L_002000"
     CDS             812..1819
                     /locus_tag="ACL73L_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HGS0772906.1"
                     /translation="MQNLIIGIQKRKNRITLFSSLFLLIIISLSFFILLIGDESYSFS
                     TLIKVLNSETVPGASFSIMEIRLPKLLAGIIAGWSFGLAGFIFQTMLRNPLASPDIIG
                     VTSSSSIAAVFCILVLKTNSLTTGIISITCGLTSSLILFLLAKKDGFSAARLIILGIG
                     FQAVTRAGTSFLLLKVARYELQEVMRWLSGSLSFTKLDDIPLVLIVSIIATILVLFFN
                     KRLEIIELGEEIAIGLGANPELSRLVLIFCAVSLTAFSTSITGPIACISFLAGPIALN
                     IGKKRSPILAGLVGILLVLLSDIFSQNILPARYPVGVVTGLLGSPYLIYLLIKMNRRN
                     I"
     gene            1819..2613
                     /locus_tag="ACL73L_002001"
     CDS             1819..2613
                     /locus_tag="ACL73L_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0772907.1"
                     /translation="MKGLWSNNLTCGYDEKIILENINIKIPEEKISVIIGSNGCGKST
                     LIKTLSRLIKPLEGEVLLDNKSINSYKEKDLAKHIAILPQSPIIPESITVADLVSRGR
                     FPYRKPFKSLGKDDLEIINRSMVKANVEDLANNLVEELSGGQRQRVWIALALAQDTSI
                     LLLDEPTTYLDISYQIELLDLLTDLNQKYKTTICMILHDINLTARYADYLFAIKEGKL
                     VAEGKPEDILNDKLVKDIFNLEAKIIRDPISNSPLMIPIGKHHINS"
     gene            2764..2948
                     /locus_tag="ACL73L_002002"
                     /pseudo
     CDS             2764..2948
                     /locus_tag="ACL73L_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612340.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1079 a          465 c          527 g         1001 t
ORIGIN      
        1 ttgcaatttt agcaggttct agtcttgcca taagcggtgt attgatgcaa tcagttacta
       61 gaaacccaat agctgatcca ggtatactcg gtataaacac aggagcaagt cttagtgtag
      121 taattggtct ttctttttta ggaatttcat caagcataag ccatataagt tttgcaatca
      181 ttggtggctt agtaagtgca atttttgtat acgcgattgc tgtaagcgga aaagcaggcc
      241 ttacccctat aaaacttgcc ttatcaggaa cttgtgttag tatggcttta agcagttttg
      301 taagtttttt aattttaccg aataataacg tcttagacaa atttagattt tggcaaatag
      361 gtagccttgg agcagctaca ttatcttcta tatctacact actacctttt ataattttag
      421 gtcacttgat agctatattt atttcatcag atttaaacgc tttagctatg ggtgatgaaa
      481 tggctgttgg tcttggagtt aatgttaata ggataagatc acttgcaata attgcaagtg
      541 tgcttttatg ttcaagtatt actgcaattg gtggacctat tggcttcgta ggtcttatag
      601 ttcctcactt ttgtggctta tttataagca aagatatacg cacaatgacc atttcttcat
      661 cttttatagg tgcagagctc ttgcttatat gtgatataat cggccgtatg ttaggtaaac
      721 caggtgaaat tgaagtaggg ataattactg caataatcgg gggtccagta cttatttatg
      781 taactatgaa aaatagaggg ggtaataact aatgcaaaat ttaattatag gtattcaaaa
      841 aagaaaaaat agaataacac tattttcctc actattttta ttaataataa tcagtctatc
      901 atttttcatt ttacttatcg gagatgaaag ttattctttt tcaactttga ttaaagtctt
      961 aaatagtgaa actgttcctg gagctagttt ttcgattatg gaaattagat taccaaaatt
     1021 attagcagga attatagctg gctggtcttt tggattggca ggatttatct ttcaaactat
     1081 gttaagaaat cctcttgcaa gtcctgatat aatcggtgtc acaagttctt catctattgc
     1141 agcggtcttt tgcatattgg tattaaaaac aaatagttta actactggaa ttatttcaat
     1201 aacttgtgga ctaacatcat ctttaatatt atttttacta gctaaaaaag atggtttttc
     1261 agcagcaaga ctgataatat taggtattgg ttttcaagct gtcacaagag caggcacctc
     1321 atttttattg ttgaaagtag caagatatga attacaagaa gttatgagat ggctcagtgg
     1381 ctctttatct tttacaaagt tagatgacat accccttgtt ctaatagtaa gtattattgc
     1441 tactatatta gttttatttt ttaataaaag actagaaatt attgaacttg gtgaagaaat
     1501 agcaatcgga cttggagcaa atcccgagct ttcaaggctt gttttaattt tttgcgctgt
     1561 atctttaact gctttttcta cttcaattac aggaccaata gcttgtatat cttttttagc
     1621 tggtcccata gccttaaata ttggcaagaa aagaagccca atattagctg gattggttgg
     1681 aattttacta gttttgttat cagacatatt ctctcaaaat attttaccag ctagatatcc
     1741 agtaggtgtt gtaactggct tgttaggttc accatattta atatacttac taataaaaat
     1801 gaacaggagg aatatataat gaaaggtctg tggtcaaata atcttacctg cggttatgat
     1861 gaaaaaataa tcttggaaaa tataaatata aaaatacctg aagaaaaaat atcagttatt
     1921 attgggtcaa atggttgtgg gaaatcaaca ctcattaaaa ccttgtctcg acttataaag
     1981 ccattagagg gagaagtatt gcttgataat aaatcaatta attcttataa agaaaaagat
     2041 ttagcaaaac acatagctat attacctcaa tctccaataa tccctgaatc aataacagta
     2101 gctgatcttg taagccgtgg tcgtttcccc tacagaaagc cttttaagag tcttggaaaa
     2161 gatgaccttg aaataataaa cagatcaatg gttaaggcca atgttgaaga tctagcaaat
     2221 aacctagttg aagaactttc tgggggtcaa aggcaaagag tatggatagc tctagcccta
     2281 gcccaagata caagtatcct acttttagat gagccaacta cttacttgga tatctcatat
     2341 caaatagaac tattagacct cttgactgat ctaaaccaaa aatataagac aaccatttgc
     2401 atgattttgc acgatataaa tctaacagca agatacgctg attacctatt tgcaattaaa
     2461 gaaggtaaac ttgttgcaga gggaaagcct gaagatatac taaatgataa actagttaaa
     2521 gatatcttta atcttgaagc aaaaattata cgtgacccta tttccaattc gcctctaatg
     2581 attcctattg gcaagcacca tattaactct taataattga atattggaat tcatttaaaa
     2641 gtcgcaaatt ttgaactgac cctcaaaagt taggccaaaa aactaactaa aggaggtcag
     2701 ttttttaatg gcaaatacag cacagaattt aagataaaat tagtaaaaga atatcatgaa
     2761 tgaaaaatag gatatagaga ataacagcaa tattaaaaag atcaggactg ataataaaca
     2821 aaaagaaagt acaacgacta gttcaaaagc taaaacttca agtaaaaagt tattcaagaa
     2881 aatctagaaa atactcatcc tacaaaggac aggtaggaaa aatatcagac aacaaaataa
     2941 aaagaaatat ttattaccga aaaaaagggg aaatcgagtt aagtaccaac aggctcatct
     3001 attccaaaaa gatatttact cgatggaaaa actcctagaa ttagtgctac aaatattgac
     3061 aatggtatct ta
//
LOCUS       DBERDK010000113         3025 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.113, whole genome shotgun sequence.
ACCESSION   DBERDK010000113 DBERDK010000000
VERSION     DBERDK010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.113"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            29..834
                     /locus_tag="ACL73L_002003"
                     /pseudo
     CDS             29..834
                     /locus_tag="ACL73L_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076725.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            856..1595
                     /locus_tag="ACL73L_002004"
                     /pseudo
     CDS             856..1595
                     /locus_tag="ACL73L_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546805.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
     gene            1608..2774
                     /locus_tag="ACL73L_002005"
     CDS             1608..2774
                     /locus_tag="ACL73L_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HGS0772908.1"
                     /translation="MNYLVISPYYPQNFQQFTIELANKGITVLGIGQESYEQLDEPLR
                     NSLTEYFRVDNLENIDEVKRAVAFLFYKHGPIGRIESHNEYWLELDATLREQFNVFGA
                     KPEDLKKTKYKSEMKKLFKKAGVPVVPGAVIKTEADVDQAVKEIGLPMIAKPDNGVGA
                     AATFKLETEDDINHFKQEWDHSTLYFFEKFVTSSEICTFDGLVDKDGKIVFSTTFDYV
                     YTPLDLMIYKMDNSYYVLKDMDPKLRKYGEAIVKEFGMKERFFHIEFFREGDDYITIE
                     YNNRPAGGFTIDVYNFAHSLDLYRGYAAIVAGEEFPASDFETQYCLATSRRANAHYVY
                     SEEDLLAKYSQQFKVKKVMPAAFAELQGDYLYMLTTPSRQEMEQMIADFGQRQE"
BASE COUNT          884 a          592 c          669 g          880 t
ORIGIN      
        1 aattttgaat atagagagtt ttctgacaat gaatcaatcc tacttttatc taaaaatgaa
       61 agaacacaaa ctcaaggttc cttatacagg taaggagcgc cgtgtacgta ttcttcttcc
      121 taaagattat gagaaagata cagaccgttc ttatcctgtt gtatactttc atgacgggca
      181 aaatgttttt aatagcaaag agtctttcat tggacattca tggaagatta tcccagctat
      241 caaacgaaat ccggatatca gtcgcatgat tgtcgttgct attgacaatg atggtatggg
      301 gcggatgaat gagtatgcgg cttggaagtt ccaagaatct cctatcccag ggcagcagtt
      361 tggtggtaag ggtgtggagt atgctgagtt tgtcatggag gtggtcaagc cttttatcga
      421 tgagacctat cgtacaaaag cagactgcca gcatacggct atgattggtt cctcactagg
      481 aggcaatatt acccagttta tcggtttgga ataccaagac caaattggtt gcttgggcgt
      541 tttttcatct gcaaactggc tccaccaaga agcctttaac cgctatatcg agtgccagaa
      601 actatcgcct gaccagcgca tcttcatcta tgtaggaaca gaagaagcag atgatacaga
      661 caagaccttg atggatggca atatcaaaca agcctatatc gactcgtcgc tttgctatta
      721 ccatgatttg atagcagggg gagtacatct ggataatctt gtgccatcca tagtgaaatc
      781 ccttggtcag aaaatctacc agattgtctg agattttttg cagaaaaatg gtaagttaag
      841 aaaggaaaaa acgaaatgca tattgaacat cttagccact ggagtggtca tcctaaccgt
      901 gaaatgtacc ttaaccgtta tggacatggt gggattccag ttgtggtctt tgcttcatca
      961 ggtggtagtc acaacgaata ctatgatttt ggcatgattg atgcctgtgc ttcctttatc
     1021 gaggaaggcc gtgtccagtt ctttacccta tctagtgtgg atagtgagag ctggttggct
     1081 acttggaaaa atgctcatga ccaagcggaa atgcaccgtg cctacgaacg ttatgtgatt
     1141 gaggaggcca ttcttttatc aagcacaaga caggttggtt tgatggcatg atgacgacag
     1201 gttgctctat gggagcctat catgcactta atttcttcct ccagcatcca gatgtcttta
     1261 ccaaagtgat tgctctcagt ggtgtttacg acgcacgttt ctttgtcggt gattactaca
     1321 acgatgatgc tatttaccaa aactcgccag tagattatat ttggaaccaa aacgacggct
     1381 ggtttattga ccgttaccgt caggcagaga ttgtgctgtg tacggggctt ggagcctggg
     1441 aacaagatgg tttgccatcc ttttacaagc tcaaagaagc ctttgacaag aaacaaattc
     1501 cagcctggtt tgctgaatgg ggacatgatg tcgcccatga ctgggaatgg tggcgtaaac
     1561 aaatgcctta tttcctcggt aatctctatt tataaaagga gttacctatg aattaccttg
     1621 ttatttctcc ctactatcca caaaactttc aacagtttac catcgaacta gctaataaag
     1681 gcatcacagt cttgggaatt ggtcaagagt cttacgagca attggatgag cccttgcgca
     1741 atagcttgac cgagtatttt cgtgttgata atcttgagaa catagatgaa gtcaaacgtg
     1801 cagttgcttt tctcttttat aaacatggtc caattggccg catcgagtct cacaatgaat
     1861 actggcttga gctagacgca acactcagag aacaattcaa tgtttttggt gccaaaccag
     1921 aggatctcaa aaagacgaaa tataagtctg aaatgaagaa acttttcaaa aaagcaggtg
     1981 ttcctgtggt acctggagct gttatcaaga cggaagcaga tgttgatcaa gcagtgaaag
     2041 aaatcggtct tccaatgatt gccaaacctg ataatggagt gggagcagcc gcaaccttta
     2101 aacttgagac agaagacgat atcaatcact tcaagcaaga atgggaccat tcaacccttt
     2161 atttctttga aaaatttgtc acttccagcg aaatctgtac ctttgacggg ctcgtggaca
     2221 aggatggaaa gattgtcttc tcaacaacct ttgactacgt ctatacaccg cttgacctca
     2281 tgatttataa gatggacaat tcttattatg tgctcaagga tatggatcct aaactgcgca
     2341 agtatgggga agcaattgtc aaagaatttg gtatgaaaga acggtttttc catattgagt
     2401 tcttccgtga gggggacgat tatattacca tcgagtacaa taaccgccct gcaggtggtt
     2461 ttaccattga tgtttataac tttgctcatt ccttggacct ttatcgtggc tatgcagcta
     2521 ttgtcgcagg agaggagttc ccggcgtcag actttgaaac tcagtattgt ttggctactt
     2581 ctcgccgtgc aaatgctcac tatgtttatt cagaagagga tttgcttgcc aaatatagcc
     2641 agcagttcaa ggttaaaaaa gtcatgccag ctgccttcgc ggaacttcaa ggagattacc
     2701 tgtatatgct gaccactccg agtcgacaag aaatggagca gatgattgca gatttcggac
     2761 aacgtcaaga ataagaacta tcggattaag gaaattcact cccttaatcc ttttgttttg
     2821 tctggtaaaa aataagagca tcctaacaag gtagctatca taaaacttgt tcgataacta
     2881 tttgaagcag gattaggtgg ctagaaattg aattttaaca tttcaattga gtcatagtat
     2941 tgtgttttcg tatccttaaa tcagctaaaa ggatccatga cgacacctat acgatatagt
     3001 tttcaggata ccaaacaagt ctatt
//
LOCUS       DBERDK010000114         2957 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.114, whole genome shotgun sequence.
ACCESSION   DBERDK010000114 DBERDK010000000
VERSION     DBERDK010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.114"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(94..1380)
                     /locus_tag="ACL73L_002006"
     CDS             complement(94..1380)
                     /locus_tag="ACL73L_002006"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000205044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HGS0772909.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGIGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGARNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
     gene            complement(1611..1847)
                     /gene="comW"
                     /locus_tag="ACL73L_002007"
     CDS             complement(1611..1847)
                     /gene="comW"
                     /locus_tag="ACL73L_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma(X)-activator ComW"
                     /protein_id="HGS0772910.1"
                     /translation="MLQKIYEQMANFYDSIEEEYGPTFGDNFDWEHVHFKFLIYYLVR
                     YGIGCRRDFIVYHYRVAYRLYLEKLVMNRGFISC"
     gene            2107..2922
                     /locus_tag="ACL73L_002008"
                     /pseudo
     CDS             2107..2922
                     /locus_tag="ACL73L_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259226.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1021 a          649 c          418 g          869 t
ORIGIN      
        1 aaagtttagg atatttgtta caacaagtta ggaggtcttc ttgtaactgt ctatagtata
       61 cccgacctat cttaaacaaa tcttaaaaat ctcttaggac caaacacttt ctaaaatatt
      121 tgtttgttca cgaccaggac ctactgagaa agtagaaata cgaacgccaa ccaattcact
      181 cacacgacga acatagttac gcgcattctc aggaagatct tccaaattgc gagctccggt
      241 aatatcttct gaccaacctg gcaactcttc atagataggc ttgcaacgtt tcaattgctc
      301 aagactagct ggatagtagt caatacgttg accgtcaaga tcataggcca cacaaatttt
      361 cacagtatcc aagccgctca aaacatcaat agagttcaaa gaaaggttag taataccaga
      421 aacacgacgg ctatgacgca tcacaactga gtcaaaccaa cctacacgac gtggacgacc
      481 agttgttgta ccatattcat gacccacttc acggatacgt tctcccactt catcaaacaa
      541 ctcagttggg aaaggaccat ctcctacacg actcgtataa gctttacata cacctacaac
      601 cttgtcaatc ttgcttggac cgataccaga accaattgtc acaccaccag ctacagggtt
      661 tgatgacgta acaaatggat aagtaccttg atcgatatct agcataacac cttgtgcacc
      721 ttcaaaaagc acacgtttgc cattatcaag cgcatcattc aagataacag atgtatctat
      781 cacgtatttc ttgatttgtt gaccatattc gtaatattct tcaaaaatat catcgaaaac
      841 aatcgcttta ctgtcataca atttttcaaa aagacgattc ttttcagcaa ggttacgttc
      901 taaacgctca cggaaaatat ctttatctaa aagatctgca atacgaattc caacacgagc
      961 agccttgtcc atataagctg gaccaattcc cttaattgtc gtaccaatct tattgtcgcc
     1021 cttagcttct tcttgcaagc gatccaactc gatatgataa ggcaaaataa catgcgcacg
     1081 atcagaaata cgcaagttat cagttgttac accttcctca tgaagatagc tcaactcttt
     1141 tacaagagat ttaggattta caaccatacc attcccaatg acagatattt tttcagggaa
     1201 gaaaatccca gatggaatca agtgcaactt aaatttctta ccgtcaatca caatcgtgtg
     1261 accagcatta tcaccacctt ggtaacgtgc aattacttct gcattcgctg aaaggaagtc
     1321 tgtaatcttc cctttacctt catcacccca ttgggtacct acaacaacaa ctgaagtcat
     1381 aattttgtct gagccctcag gctcttcctt tctcacatac atggcaggac tctcacctgc
     1441 aattatatct tacaatttat tataataaaa aatcgccttt ttatcaagaa gaaacaatag
     1501 aaagatttgc tatttccaac tattaaaaaa tgatttagaa aaattactag ctatttacta
     1561 ttatctttcc ataaaagagt aaattagttc ggaaatttac taaaattacc tcaacaagaa
     1621 ataaaacccc gattcattac caatttttca agatacaaac gataagcaac acgataatgg
     1681 taaacgataa aatccctacg acaaccaatg ccatatctca ctaaataata aattaaaaat
     1741 ttaaaatgaa catgttccca gtcaaaatta tcaccaaatg taggaccata ctcttcttca
     1801 atactatcat agaaattagc catctgctca taaatttttt gtaacataat caaatactcc
     1861 ttttcttttt tataaactta ttctaacaaa aaattttaca cattcactat caattcctga
     1921 attgttaaaa taccagcctc aacaagataa aaataggaaa agttgacaga atagaaacaa
     1981 atttgctacc taaatttcaa acgattaaag ttctaaaaag accgccttaa aacttttggg
     2041 taaatcctat tagaactata atatttttat ataggtatta tggttctgat tttctagtat
     2101 aaaaaaattc aaactagaat tcttcttata aaagtaaaag gccgttttaa gtgctatcat
     2161 tgctcaaaaa tggaggtcgc tgagacttct atcgtcaaga agaatcatca aattccttgt
     2221 atcatcaacc aaaagattgc tcagaagcta attgaaaaga cttctatgac cgacattgat
     2281 catcagttgt ctatttcaat ttcaacttca actgtcattc gcaagatcaa tgattttcac
     2341 tttgagcatg atttttcgcg tcttcctgag attatgtcct aggacgttga aacagtccgg
     2401 ggagtgactg tttcaatcgg gagatggaga tgagctttat tgctcaagac tttgataagc
     2461 tcaatatcat aactgttctt gagagcagaa cacaagccat catccgaaat cccatgaata
     2521 caaggctatc aagcgatact gaaagctcat tcaacaagat agtcagaaac tgagtgataa
     2581 acgattttat cgccctactt ttcgtatgca cttgactaat aaagaaattc tagataaact
     2641 tttgagctat tccgaagact tgaaacacca ctataatctc tatcagctct tgcttttcca
     2701 cctctttttc agactctgtt taaaaccttc ctcaaagata aagagaaaat cgtcaacgcc
     2761 cttcaattac cttattccaa cgccaaactg gaagccacca ataatctcat caaacttatc
     2821 aagcacaatg cctttggttt taggaacttt gaaaacttca aaaaagaaag gacgaaattt
     2881 gtcctttcta gattttcgct ttcttcaacc cactacagtt gacaaagaac cgaaaaatgt
     2941 ctcgcctcta atatggt
//
LOCUS       DBERDK010000115         2920 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.115, whole genome shotgun sequence.
ACCESSION   DBERDK010000115 DBERDK010000000
VERSION     DBERDK010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.115"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(134..592)
                     /locus_tag="ACL73L_002009"
     CDS             complement(134..592)
                     /locus_tag="ACL73L_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HGS0772911.1"
                     /translation="MKKQVFHDAAAGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWAAIGMLFNFGNRLFSRDWSMLRATLTHFFLMLAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFAIVYLIIWAILYKREAKKVDHINQLLEHRK"
     gene            complement(589..1029)
                     /locus_tag="ACL73L_002010"
     CDS             complement(589..1029)
                     /locus_tag="ACL73L_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HGS0772912.1"
                     /translation="MKVELQISETYEEEKLIVQAPQPTDKVQKVIEFAENLDQREKIK
                     GKIDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRLYQVLKLLPTNFIQISQSE
                     IINIDSISHLKLTPNGLVEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            1613..2479
                     /locus_tag="ACL73L_002011"
     CDS             1613..2479
                     /locus_tag="ACL73L_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGS0772913.1"
                     /translation="MGTLLATRLKNRRKELKMSQRELAEGICKQGQISRLESGEFTPG
                     ADFLHALAKKLKVSMDYFFDEQVVEKVEELSEFKKLAQTFITNRNYESLKYIYELENV
                     KVHRLSLADKIYMEWIKSLIDFYFYGQKEDAIVRLEGVLSQLSVSDLNYLQVSNTLFN
                     FYYDIGDLGRFNKIREKLEYRVNQLNLSTIEELELSIKFNYNVCRYLWLQKNIEEAIT
                     KITATIKQCKAYRTNYLLADLYLLMGNVSENFSSKSSVKEYFETAHFLYKLEENMSMA
                     LKVEHYIADITE"
     gene            2629..2763
                     /locus_tag="ACL73L_002012"
     CDS             2629..2763
                     /locus_tag="ACL73L_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772914.1"
                     /translation="MRSLFKKIVAVLVIGLILLGIARTPQVHKIARGIDPGPANGQAL
                     "
BASE COUNT          961 a          440 c          615 g          904 t
ORIGIN      
        1 aaacattgtt agaaatcgat ttgactgtcc tgatcgattt atcctgttct tatttcattt
       61 cactatagta aggtgcagca ggataaagaa aaaccgagaa gtttctttct cggatttgct
      121 tattatgagg ttcctatttt ctatgctcca agagttgatt gatgtgatct acttttttag
      181 cctctctttt atagagaata gcccagatga tgagatagac aatcgcaaac tcgataatga
      241 gctggaggta gaaaatccag tggaagggga accaaccagc aagagttgct agtgggacaa
      301 agccagccag cataaggaag aaatgagtca gagtggcacg aagcatgctc cagtcacggc
      361 tgaataagcg gttgccaaag ttgaagagca taccgatggc tgcccagata agtgtgcagt
      421 agagcaagac cagggcaccg tgaacctgat gctgatccat cacttggccg atgagagagt
      481 agggatttag tggtgcgtag gtatttggtg cataaatgag tgaaaagagg atggagagga
      541 tgaggccgat aagaacaccg gcagctgcat cgtgaaatac ttgttttttc atagttctaa
      601 tttctccttg atggttttta gataacggcg tgaagagtag gtgaagcttt cgtttttcaa
      661 gaaaatttct accagaccgt ttggcgtgag cttgaggtga gagatggaat cgatattgat
      721 gatttctgat tgggaaattt ggataaaatt ggttggcaag agtttaagaa cctgatagag
      781 tcgcaaatca atgctgtagg tctgactcgc ggtttctgct agaaccttcc gattctcgat
      841 atagaagcgc tgaatcttgc caatctcaac tagatagacc tgatcatcga ttttcccttt
      901 gattttttct ctttggtcca gattttctgc gaactcgatg actttctgaa ctttatctgt
      961 cggctgaggt gcttggacaa tcagcttttc ctcctcgtaa gtctcactaa tctgtaattc
     1021 tactttcata aatttctctc cttgtttttc atacaaaatt aatctcactt cttgtacacc
     1081 tttattaata ctcttcaaaa atctcttcaa accacgtcag cgtcgtcttg ccgtaggata
     1141 gttgttgact ttgtcagttt tatctacaac ctcaaaacag tgttttgagc tgtctgcgcc
     1201 tagctttcta gtttgctctt tgatttttat tgagtatacc actattttac tccctctggc
     1261 aaggtacttt gtctatgtgg agggattggg ctcctatgtg gtggagcttt tctgttcttt
     1321 ctgaaatatg gtataatagc actaatcaat ttctaggaaa atagatacag aaaggggctg
     1381 aaagaactag gggaacagtt gtaattagaa ggtgataggt atgaaaagtg gcgttgaagc
     1441 tggataagtt tataaagagt ttgaatgaag aaatgaaaag taaataagtt gatgatgaat
     1501 tgcaaaaata gattttatat acaatttgta tctttcgaat cattgatttg aattttagat
     1561 ttaatcatgt tacaattaaa actgaaatca aatagaagag gaaaatgcaa acgtgggcac
     1621 actattagca acaagattaa aaaatagacg gaaagaatta aaaatgtctc agcgagaact
     1681 agctgaaggt atctgtaaac aggggcaaat cagtcgatta gagagcggag agtttacccc
     1741 tggagcggat ttcctgcatg cccttgctaa gaagttaaaa gtcagtatgg attatttttt
     1801 tgatgagcag gttgtagaga aagttgagga gttatcggaa tttaagaaat tagcacagac
     1861 atttattaca aatcgcaatt atgagtcttt gaaatatata tatgaattgg agaatgtaaa
     1921 agttcaccgc ctatctttag cagataaaat ctacatggaa tggataaaat ctctgataga
     1981 tttttatttt tacggacaga aagaagatgc gattgtacga ttagaggggg tattatcaca
     2041 gttaagtgta tctgatttga actatctcca agtttcaaac accctattca atttttacta
     2101 tgatattgga gatttaggtc gtttcaataa aattcgtgaa aaactagagt atcgagtaaa
     2161 tcaactcaat ttgagtacaa tcgaagagtt agagctatct attaagttta actacaacgt
     2221 ttgtcgttat ctatggttac agaaaaatat tgaagaagct attactaaaa ttacagctac
     2281 tataaaacaa tgtaaggcat acagaacgaa ttatcttttg gctgatttgt acttattgat
     2341 gggaaacgta agtgaaaatt tctcttctaa aagttcagtg aaagaatatt ttgagacagc
     2401 tcatttcctt tacaaattag aggaaaacat gtcaatggct ttgaaagttg agcattacat
     2461 tgcagatata acagaataat gaatacggtg ttcaagtgag catcgttttt caaacccagt
     2521 ttagatacaa aaaagtatca aacaaaaaaa aataaaaaaa cattataaaa atacttgaca
     2581 agctaatttt gaagtgataa aataaatgca agaaaaaagg agaggtatat gagaagttta
     2641 tttaagaaaa tagttgctgt attagttatt ggattaattc tgttagggat tgcgagaaca
     2701 ccccaagttc ataagatagc tagagggatt gatccaggtc cagcgaatgg gcaagctctc
     2761 taactgttca gactaatcta taacgacatt atgaggtttg ataagatttc tcctcaccgg
     2821 ataaaaatct taaattttat aatagaggga gaaattaaat tgaagtttaa gaagaagagc
     2881 ttaaagccta agaaaattcg ttttgtacta ggttacaatg
//
LOCUS       DBERDK010000116         2909 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.116, whole genome shotgun sequence.
ACCESSION   DBERDK010000116 DBERDK010000000
VERSION     DBERDK010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.116"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..1574
                     /locus_tag="ACL73L_002013"
     CDS             <1..1574
                     /locus_tag="ACL73L_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HGS0772915.1"
                     /translation="LKSDLYEIIKEGIRFNFENPIFDMKRSTNEFIGRSAMVTKLLCI
                     YSEGDPFFGVNINSQKEFWNHFVSQTNQGGPYLQNHKIIELVSKTYPELEPSKLGTML
                     FEYSKLFMENKEDNSTMDLSNNFRHQLTQSLLKSPNLILRGAPGTGKTYLAKEIAKEL
                     TDGNEDQIGFVQFHPSYDYTDFVEGLRPVSNGDGAIEFRLQDGIFKDFCQKAKETQLI
                     GGQDNFDEAWDSYLEYINVAEEKEYITKTSYLSVNSRQNLSVNYDSGVPGWSLPSKYV
                     YELYKDKNYNKQEYYKSGGKTVLETLRKRFGLKDYVSPTEIDTDKKFVFIIDEINRGE
                     ISKIFGELFFSIDPDYRGEKGSVSTQYANLHETDEKFYIPENVYIIGTMNDIDRSVDT
                     FDFAMRRRFRFVEVTAEGQVGMLDKELNIHAEEAKIRLRNLNAAIENVQELNSHYHIG
                     PSYFLKLKDVDFDYELLWSDYIKPLLEDYLRGSYDEVETLETLKKAFELTNNEQKDQA
                     VADDNEGDENDDADY"
     gene            1561..2877
                     /locus_tag="ACL73L_002014"
     CDS             1561..2877
                     /locus_tag="ACL73L_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HGS0772916.1"
                     /translation="MRITDNQHKIIKEKFVEEYPKLSNLLLDRTLESLSQDERIFIFP
                     NDLTHTPDLDKDQKIFETVNQKIKTGNVIGFLGYGQERLTISSRFSDESNDHFLHYLL
                     NKVLHINLTSLDVALSREERLYQLLMYLFPKYLQAAIRKGLYKEYQRFSHNDSHVKGV
                     IDVRNHLKKNLPFTGNIAYTTREFTYDNPLMQLVRHTIEYIKNQKSIGQGVLDNLSTS
                     RENVSEIVRVTPSYKLADRAKITRRNQSKPIRHAYFHEYRNLQELCLMILNQEKHGLG
                     YQDQKIYGILFDVAWLWEEYVYTLLPKGFVHPRNKDKTDGISVFSVGKRKVYPDFYDR
                     ERKIVLDAKYKKLELTEKGINREDLFQLISYSYILKAEKAGLVFPSKDKVIDNEMGNL
                     AGYGAQLKKWSIRIPQNASFYSAFCKMMENSEENFKAIIDEEVGRK"
BASE COUNT          993 a          454 c          560 g          902 t
ORIGIN      
        1 aattaaaatc agatttgtat gaaattatca aagaaggtat tcgttttaac tttgaaaatc
       61 ctatttttga tatgaaaaga tcaacaaatg aatttattgg tcgttctgct atggtgacaa
      121 aattactttg tatctattct gagggagatc ctttctttgg tgtaaatatt aatagtcaga
      181 aagaattttg gaaccacttt gtttctcaga caaatcaagg tggaccttat ctgcaaaatc
      241 ataaaataat tgaactggtg tccaaaactt atcctgagtt ggagccatcg aaattaggaa
      301 ctatgctttt tgagtattct aagcttttta tggaaaataa ggaagacaat agtacaatgg
      361 atttatcaaa caattttcgt catcaattaa ctcaatctct attaaagtct ccaaacctta
      421 tccttcgtgg tgctcctggc acaggaaaga cttatcttgc taaagaaatt gccaaagaat
      481 taacggatgg caacgaagac caaatcggat ttgtacaatt tcacccatca tatgattata
      541 cggattttgt agaaggttta agaccagtat caaatgggga tggagctatt gagtttaggc
      601 tacaggacgg tatttttaaa gatttttgtc agaaagcaaa agaaacccaa ttgattggag
      661 gacaagataa ttttgatgag gcttgggatt cttacttaga atatataaat gttgctgaag
      721 aaaaagaata tataacaaaa acatcttact tatctgttaa tagtagacaa aatttgtcag
      781 taaattatga tagtggtgtt ccaggatggt cactacctag caaatatgtt tacgagttgt
      841 ataaagataa aaattataat aagcaagaat actacaaaag tggtggaaaa actgtcctag
      901 aaacattgag aaagagattt ggtttgaaag actatgtttc cccaacagaa attgatactg
      961 ataagaaatt cgtcttcatc atcgatgaaa tcaatcgtgg ggagatttct aagatttttg
     1021 gcgaactctt tttctctatc gaccctgact atcgtggtga aaaaggaagt gtttctaccc
     1081 aatatgcaaa tctacacgaa actgatgaaa agttctatat ccccgaaaat gtttacatca
     1141 tcggaactat gaatgatatt gatcgttcag tggatacctt tgattttgct atgcgtcgtc
     1201 gttttcgttt tgttgaagtt actgccgagg gtcaagttgg catgttggat aaagagctga
     1261 atatccatgc agaagaagca aaaattcgtc taagaaactt gaacgctgct atcgaaaatg
     1321 ttcaggaatt aaacagtcat tatcatattg gaccaagtta ttttcttaag ttgaaggatg
     1381 tagattttga ctatgaatta ctctggtctg attatattaa gcctctccta gaagactact
     1441 tgcgaggttc ttatgatgag gttgaaactt tggaaacttt gaaaaaagca tttgaactga
     1501 caaataatga gcaaaaagat caggcagtag ctgatgacaa tgaaggcgat gaaaacgatg
     1561 atgcggatta ctgataatca acacaagatt attaaagaaa aatttgttga agaatatcct
     1621 aaactaagca atcttctttt agacagaacc ttggaaagtc tatcccaaga tgaacgtatt
     1681 ttcatttttc caaatgattt gactcatact cctgatttgg ataaggacca aaagattttt
     1741 gaaacagtca atcagaaaat caagacaggg aacgtgattg gttttcttgg atatggtcag
     1801 gaaagattaa cgatttcctc acgattttct gatgagagta atgaccattt tttgcattat
     1861 ctcttaaaca aggttcttca tatcaatctc actagtttag atgttgcttt gtctcgtgaa
     1921 gagaggcttt atcaactttt gatgtatctc ttccccaagt atctacaagc tgctattcga
     1981 aaaggtcttt ataaggaata ccagagattt tctcataacg acagtcatgt taagggagtg
     2041 attgatgtaa gaaaccatct caagaaaaat cttcctttca cgggaaatat tgcctacaca
     2101 acgagagagt tcacctatga taatcccctc atgcagttgg tccgtcacac tattgaatac
     2161 attaagaatc agaaaagcat tggtcaaggg gtactagata atctctcaac tagtcgtgaa
     2221 aacgtatctg aaatcgtgcg tgtaacgccc tcttataaac tagctgatcg tgctaagatt
     2281 actcggagaa atcaatctaa acctatacgt catgcatact ttcacgagta cagaaactta
     2341 caagaacttt gtctgatgat cctaaaccaa gaaaagcacg gtttagggta tcaagatcaa
     2401 aaaatctatg gtattctctt tgatgttgcc tggctttggg aagagtatgt ttacaccttg
     2461 ttgccaaaag gttttgtaca tcccagaaat aaggataaga cggatggaat ttcagtattt
     2521 tctgttggga aacgaaaagt atatccagat ttttatgaca gagaacgaaa gattgttcta
     2581 gatgcaaaat ataaaaaact ggaattgact gaaaaaggaa tcaaccgtga ggacttattc
     2641 cagctgattt cctattctta tattttaaaa gctgagaagg ctggacttgt ttttccgagc
     2701 aaggacaagg ttattgataa tgagatggga aacctagcag gttatggagc tcaattgaag
     2761 aagtggtcta ttcgaatccc tcagaatgcc tcattctata gtgcattttg taaaatgatg
     2821 gaaaattcag aagaaaattt taaagcgatt attgatgaag aagtggggag aaaataattt
     2881 cccctctttc ttttattttt aacacaagc
//
LOCUS       DBERDK010000117         2896 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.117, whole genome shotgun sequence.
ACCESSION   DBERDK010000117 DBERDK010000000
VERSION     DBERDK010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.117"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(43..212)
                     /locus_tag="ACL73L_002015"
                     /pseudo
     CDS             complement(43..212)
                     /locus_tag="ACL73L_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558122.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(290..1600)
                     /locus_tag="ACL73L_002016"
     CDS             complement(290..1600)
                     /locus_tag="ACL73L_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HGS0772917.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNVATTNGTLDVLAQHILYRTRTHPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGRTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYQKTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTTIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
     gene            1837..2709
                     /gene="nadC"
                     /locus_tag="ACL73L_002017"
     CDS             1837..2709
                     /gene="nadC"
                     /locus_tag="ACL73L_002017"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HGS0772918.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAILLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
BASE COUNT          877 a          496 c          671 g          852 t
ORIGIN      
        1 cctgttctta tttcatttta ctacatctta aattgagtat gaagaaagtg cgctcccatg
       61 tcctgggaac gcactttctt catatttttc atattcttga atccatcgat aaagactatt
      121 gggatgaatt tttaaagttg aactaatcat ttttacagga tgaggtttac agcagagagt
      181 ttgaaggctt tatcaaaggt ttttcttggc ataatgactt ttcctcgttt ccacttaatt
      241 ttgtgtctac tttattatac caagtccacc cttaagttag ataataaatc taacttaagg
      301 aagctaaaag gatgagaatc caggtggtca agagtcccaa acttaagctg atggggacac
      361 ccagaataat ttgctttttg aaggcaaggc cgcgttcctc tatattggga agtgagagtt
      421 gaatgagaga accagctgat gaaaagggtg agatattagt agatagagcg ccaataacgg
      481 tggctgttgt gagtaagtga atatcaatct gaggattttg agcactgatg atagcaatga
      541 tgggaaagag ggctggagct acaacggata gggtggaact aaagagtgac atcactccgg
      601 ctatcacaca aaagaacaga ggtaaccaga aatgaggaat ggttgttgtc atgaggtgcc
      661 ctatcagtgt gactaaacct gacttgaccg ctagagacat tagtaagctc atgccgcaga
      721 gcatgataat tgtagcccag ggaaccttag ctaaaatggc ttcttgcttc cctaatttga
      781 gccttaaggc gaggcagacc atgagtattg agacaaagcc aatatcaaat gttttttgat
      841 aagtagctat ccaggcgatg tttgggaaaa tgagatgcaa caagggaaaa agccaaacca
      901 aaaccatgct gctgatcatg agcaaggtgg tttgtctttg aaccttgctg aggagtggtg
      961 gttggtcaat agtcaaggat gagtttgttc ttcccttact atagtgactg taacaggata
     1021 ataaaagcaa gacgatgagt gggtagataa tgctgacgat aaagatatga ttgccaagtg
     1081 aaaaagcttg ctcttcccat cccatttgct taaacaggcc ttgaaagaca atgcctgagc
     1141 tactggttat caaattagcc cctcctgaag ctccccaatt gacggcttga gctccaatca
     1201 aagggtgttt gtccgctttt tgacagaggg taatcgctag aggacagcaa acggccatag
     1261 tagtgaaaaa tccagcacct aaagcagaca aaagggttgc catcaggtat aaaatcatgt
     1321 agagggcgtt agggtgggtg cgtgtgcggt agagaatgtg ttgagccaaa acatcaagag
     1381 taccgttagt tgttgcaacg ttataaaaga gagagacgct aaaaatggta aaaaagagtg
     1441 aggttggcca aaaatgaaga agttctttgg ggcttaatcc catgagagtg gttgcgatga
     1501 ggtaagaaaa agcaatagcc agcaggccaa tattgatttt ggtgcggtaa ccaattccaa
     1561 tggctagagc aatggcgcta atcattatta aatgaatcat tgaattgtcc tttcagttag
     1621 aatatggaaa taggttggca attttggcgc tcaaaagacc acctgtttcc tattggtact
     1681 tgactattat acacaaaaaa aatacaattg tcttgacaat tgtattgaca cttgttagaa
     1741 ttaaaaatga ggtattcagg cttgtgattt cctatggaag ttaatagtga ttgcctctaa
     1801 tgcttacaag tgatattaaa aatagaggac ctagtgatgt caatcatttc aactgattta
     1861 accccttttc aaatagatga tacattgaaa gcagccttgc gagaagatgt tcattccgaa
     1921 gattacagta ccaatgccat ttttgatcat catggccaag ccaaggtgtc gctttttgcc
     1981 aaggaagctg gtgttttagc ggggctaacc gtttttcaaa gggtttttac cctatttgat
     2041 gccgaggtga ccttccagaa tcctcatcaa tttaaggatg gggatcgttt gactagtggc
     2101 gatttggttt tagaaatcat aggctcggtg agaagtctct taacatgtga acgcgttgcc
     2161 ttgaattttt tacaacattt atcagggatt gcttcgatga cagctgctta tgtagaagcc
     2221 ttaggcgatg attgcattaa ggtatttgat actcgaaaaa ctactcctaa tttacgtctt
     2281 tttgagaaat atgccgtgag agttggcggt ggctataatc atcgctttaa tttatcagat
     2341 gctatcctgc taaaagacaa tcacattgcg gcagtaggta gtgttcaaaa ggcaattgct
     2401 caagcgcgtg cctatgcccc ttttgtgaaa atggtcgagg tggaagtgga aagccttgct
     2461 gctgccgaag aagctgcggc ggcgggtgct gatattatca tgttggataa tatgtcattg
     2521 gaacagattg aacaggccat taccctaatt gcaggacgtt ctcggattga atgttctgga
     2581 aatattgata tgaccactat tagccgtttt cgtggtttag cgattgatta cgtctccagt
     2641 ggtagtttaa cccatagtgc taagagtctt gatttttcca tgaaggggtt aacctacctt
     2701 gatgtctaag ttgtaaaata aactaacttt ttaaaggatg tctttcctct agaacgagtt
     2761 ttatgtcaga tagtttaaac gcctcttcaa atatagtaaa atgaaccaaa aatagtacac
     2821 aatgtggtat aatcttttta tggcatattc aatagatttt cgtaaaaaag ttctctctta
     2881 ttgtgagcga acaggt
//
LOCUS       DBERDK010000118         2806 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.118, whole genome shotgun sequence.
ACCESSION   DBERDK010000118 DBERDK010000000
VERSION     DBERDK010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.118"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(1..673)
                     /locus_tag="ACL73L_002018"
                     /pseudo
     CDS             complement(1..673)
                     /locus_tag="ACL73L_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase C-terminal
                     domain-containing protein"
     gene            complement(919..1230)
                     /locus_tag="ACL73L_002019"
     CDS             complement(919..1230)
                     /locus_tag="ACL73L_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHY zinc finger protein"
                     /protein_id="HGS0772919.1"
                     /translation="MIEAQGFLVDKQTRCIHYHSKLDIIALQCYDCKKYYACYRCHDS
                     LENHPFEPYPLSLIQDKPILCGVCLKLLTYKQYKESLSCPFCFSRFNPGCQNHKERYF
                     K"
     gene            complement(1245..2531)
                     /locus_tag="ACL73L_002020"
     CDS             complement(1245..2531)
                     /locus_tag="ACL73L_002020"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HGS0772920.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFAAKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     ALIMIAATEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMEELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYDLYDMPFGKERKSL
                     QGEIPEEFSMSAVDMSMIDHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     LESFEKFEAIKQDLVDEMWKVAQRELATGFYYGIPSENEQLFGARRKIPEYKFVAEVV
                     SYDDAAQTATIRQRNVINEGDQVEFYGPGFRHFETYIEDLHDAKGNKIDRAPNPMELL
                     TIKVPQPVQSGDMVRALKEGLINLYKEDGTSVTVRA"
BASE COUNT          833 a          623 c          494 g          856 t
ORIGIN      
        1 cttttaatgc acgaaccatg tctcctgatt gaacgggttg aggcacctta atagtcaata
       61 gttccattgg atttggagcg cggtcgattt tattgccttt ggcatcatgc aaatcttcaa
      121 tataggtttc aaaatgacgg aaacctggac cataaaactc aacttggtcc ccttcgttaa
      181 tgacatttcg ttgacgaata gttgctgttt gtgccgcatc atcataagaa accacttcag
      241 cgacaaactt gtactcagga attttacgac gagcaccaaa caactgctca ttttcagatg
      301 gtgtaccata gtaaaatcct gtagccagtt cacgttgggc aaccttccac atctcgtcca
      361 ccaagtcttg tttgatagct tcaaactttt caggactttc aagataggca tccacagccg
      421 ccttgtagca gttggttact gttaatacat agtgaataga ctccatacgt ccttcgattt
      481 ttagactgtc cacaccattt tcaatcatat ctggaatgtg gtcaatcata gacatgtcaa
      541 cggctgacat tgaaaattct tctggaatct caccctgcaa actcttacgt tctttcccaa
      601 atggcatatc gtaaaggtcg tacttccaac ggcatgactg agaacatcca ccacggttgg
      661 catcacgcat actctagacg gattgttttg acaattctaa tcttttagac tttgcataat
      721 aactgttaaa cagttgaaaa ttgacccagt tttaacaagt agtcaaaata ccagataaag
      781 gaaaaaagtc tttaaaatct taaaaacgca tagtatcagg tattgaacaa tcttgatatt
      841 atgcgtttta ttgtgggaag atttacttca ttttctcttg gaattgagtt ttctcctact
      901 tcaaaactag atgatttgct atttaaaata gcgttcctta tgattttggc aacctggatt
      961 aaagcgagaa aaacaaaagg ggcaacttaa gctttcttta tattgcttat atgttagtag
     1021 ttttagacaa acaccacata aaataggctt atcctgtatc aaagataagg gatacggctc
     1081 aaaagggtga ttttctaatg aatcatgaca ccgataacaa gcataatact ttttacaatc
     1141 atagcattgt aaagcaataa tatccagctt gctatggtaa tgaatgcatc ttgtttgctt
     1201 atccactaag aaaccctgtg cctctatcat ttccttttcc tttcttaagc tcgaactgtg
     1261 acgctggttc catcttcctt ataaagattg atgagtcctt cttttaatgc acgaaccatg
     1321 tctcctgatt gaacgggttg aggcacctta atagtcaata gttccattgg atttggagcg
     1381 cggtcgattt tattgccttt ggcatcatgc aaatcttcaa tataggtttc aaaatgacgg
     1441 aaacctggac cataaaactc aacttggtcc ccttcgttaa tgacatttcg ttgacgaatt
     1501 gttgctgttt gtgccgcatc atcataagaa accacttcag cgacaaactt gtactcaggg
     1561 attttacggc gagcaccaaa caactgctca ttttcagatg gtataccgta gtagaaacct
     1621 gttgccaatt cacgttgggc aaccttccac atctcgtcca ctaagtcttg tttgatagct
     1681 tcaaactttt caaaactttc aagataggca tccacagccg ccttgtagca gttggttact
     1741 gttgatacgt agtgaataga cttcatacgt ccttcgattt ttagactgtc cacaccattt
     1801 tcaatcatat ctggaatgtg gtcaatcata gacatgtcaa cggctgacat tgaaaattct
     1861 tctggaatct caccctgcaa actcttacgt tctttcccaa atggcatatc gtaaaggtcg
     1921 tatttccaac ggcatgactg agaacatcca ccacggttgg catcacgcat actcatgtgg
     1981 tttgaaagag tacaacgtcc agagtatgaa atacacatag ctccatggac aaaggcttca
     2041 atttcaacat ctgtacgttt gcggatttca gctaattctt ccattgaaac ctcacgcgct
     2101 aaaacgacac gagtcaagcc tagctctttc cagaactcaa gggtttcata gttagtggca
     2161 ctggcttggg tagaaaggtg gatttcaagg cctggtgctt cagtcgctgc aatcataatc
     2221 aaggctgggt cagatacgat aactgctgca atcccgatat cacgcagttt acggaaccac
     2281 tcaccagcac cagcttcatt tccttcgtgc ataaccatat tagccgctac atagaccttg
     2341 gcaccatact tggccgcaaa ctgcacgcct tcttccatct gttcgaaagt aaagtttccc
     2401 gcacggctac gaagaccata ggcctgacca ccgataaaga cagcatctgc tccatactga
     2461 acagctacct ttagcttctc taaagtccct gcaggtgata aaacctcagg acgttttaat
     2521 gtttttgtca taatttctcc tatttgcaat ataaaagttt gacatttttc cttctcattt
     2581 tatagtaaat aaaggataaa atcaagccta gacggattgt tttgacaatt ctaatctttt
     2641 agactttgca taataactat taaacagtcg aaaattaccc cagttttaac aagtagtcaa
     2701 aaagtctaaa aaagtaaaaa ccagtatccc aagatactga ctcgtaaaaa tatataaatt
     2761 agtccttaga tgactactcc cactctgttg ctcctataat ggaaaa
//
LOCUS       DBERDK010000119         2764 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.119, whole genome shotgun sequence.
ACCESSION   DBERDK010000119 DBERDK010000000
VERSION     DBERDK010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.119"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(158..2242)
                     /locus_tag="ACL73L_002021"
     CDS             complement(158..2242)
                     /locus_tag="ACL73L_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000341103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGS0772921.1"
                     /translation="MDDKITVIVPVYNVENYLRKCLDSIISQTYKNIEIVVVNDGSTD
                     ASGEICKEFSEMDHRILYIEQENAGLSAARNTGLNNMSGNYVTFVDSDDWIEQDYVET
                     LYKKIVEYQADIAVGNYYSFNESEGMFYFHISGDYYYEKVYDNVSIFENLYETQEMKS
                     FALISACGKLYKARLFEQLRFDMGKLVEDGYLNQKVYLLSEKVIYLNKGLYAYRIRKG
                     SLSRVWTEKWMHALVDAMSERITLLANMGYPLEKHLAVYRQMLEVSLANGQASGLSDT
                     AIYKEFEMKKRLLNQLTVQESSEKKAIVLAANYGYVDQVLTTIKSICYHNRSIRFYLI
                     HSDFPNEWIKQLNKRLEKFDSEIINCRVTSEQISCYKTDISYTVFLRYFIADFVQEDK
                     ALYLDCDLLVTKNLDDLFATDLQDYPLAAVRDFGGRAYFGQEIFNAGVLLVNNAFWKK
                     ENMTQKLIDVTNEWHDKVDQADQSILNMLFEHKWLELDFDYNHIVIHKQFADYQLPEG
                     QDYPAIIHYLSHRKPWKDLAAQTYREVWWYYHGLEWTELGQNHHLHPLQRSHIYPIKE
                     PFTCLIYTASDHIEQIETLVQSLPDIQFKIAARVIVSDRLAQMTIYPNVTIFNGIHYL
                     VDVDNELVETSQVLLDINHGEKTEEIINQFARLGKTILSFENTKTYEAGQEVYAVDQV
                     QAMIEKLREISK"
     gene            complement(2303..2380)
                     /locus_tag="ACL73L_002022"
     CDS             complement(2303..2380)
                     /locus_tag="ACL73L_002022"
                     /inference="COORDINATES: protein motif:HMM:TIGR01167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HGS0772922.1"
                     /translation="MLLGVLAAVTGIGLVAKRRKRDEEE"
BASE COUNT          841 a          567 c          452 g          904 t
ORIGIN      
        1 taaatatctt taggtctatt tatattttat aaagcactct gatgtttgaa caactttcat
       61 atatgatgaa cggagaaatt ttataaaaaa taatccaact cttcaatctg atcactaacc
      121 gtcaaaatca gagcatctcc tactaaatga tttttcttca tttgcttatt tctctcaatt
      181 tctcaatcat tgcttgaact tggtcaacag catatacctc ctgacctgct tcataggttt
      241 tggtattttc aaaggacaaa atagtttttc caagtcttgc aaattgattg ataatttcct
      301 ctgttttttc accatgatta atatctaaaa gtacttgact ggtttctacc aattcattat
      361 cgacatctac caaatagtga attccgttaa atatagtcac gtttggataa attgtcatct
      421 gagccaatcg atcactaact attactctag ctgctatctt aaactgaata tcaggcaagg
      481 attgaaccaa tgtctcaatt tgttcaatat ggtctgaggc agtatagatt agacaagtga
      541 aaggttcctt tattggatag atgtgagatc tttgtaatgg atgtaaatga tggttttgtc
      601 ccaattctgt ccattcaagc ccatgatagt accaccaaac ttcacgatag gtttgggccg
      661 ccaaatcttt ccacggtttc cgatgagaaa gatagtgaat aatagcagga taatcctgac
      721 cctcaggcaa ttgataatca gcaaactgtt tatgaatgac aatatgatta taatcaaagt
      781 ccaattccaa ccatttatgt tcaaaaagca tattcaagat gctctgatct gcctgatcca
      841 ccttatcatg ccattcattg gttacatcaa ttaatttttg ggtcatattc tcttttttcc
      901 aaaaagcatt gtttaccaag agaacaccgg cattaaagat ttcttgacca aaataagctc
      961 tgcccccaaa atctctaaca gcagccaaag gataatcttg taagtctgta gcaaacaaat
     1021 catcaagatt tttcgttaca agtaaatcac agtccaagta gagggcctta tcttcttgca
     1081 cgaaatcagc tatgaaatag cgtaaaaaaa ctgtgtaact aatatccgtt ttataacatg
     1141 aaatttgctc agaagttacc cgacaattaa taatttctga gtcaaacttc tctaagcgct
     1201 tatttaattg cttaatccat tcatttggaa aatcgctatg aatcagataa aaacgaatcg
     1261 aacgattatg ataacaaata gacttgattg tcgttaaaac ttggtctaca tagccatagt
     1321 ttgctgctag gacaatggct ttcttttcac ttgattcttg taccgttaac tgatttaaaa
     1381 gtcttttttt catttcaaac tctttatata ttgctgtgtc agataaacca ctagcttgac
     1441 cgttggcgag actgacttcc aacatctgac gataaactgc caagtgtttc tctagaggat
     1501 atcccatatt agctagtagc gtaatacgtt cagacatagc atcaactaag gcgtgcatcc
     1561 acttttctgt ccaaactctt gatagactac cttttctaat ccgataagca taaagacctt
     1621 tatttaaata aattaccttt tccgataata aatatacctt ttgattgagg taaccatctt
     1681 ctactaattt acccatgtca aagcgcaact gctcaaacaa tcttgcctta tagagtttac
     1741 cacaagcaga tatcaaagca aaactcttca tttcttgagt ttcatacaag ttctcaaaga
     1801 tggaaacatt gtcatatacc ttctcataat agtagtctcc tgatatatga aagtagaaca
     1861 ttccttcact ttcgttgaaa gaatagtaat tcccaactgc aatatcagcc tgatactcta
     1921 ctattttttt atatagagtt tctacataat cttgctcaat ccaatcatcc gagtccacaa
     1981 aggtcacata atttccggac atattattca gaccggtgtt tcgtgcggca gaaagaccag
     2041 cattttcttg ttctatatag agaattcggt gatccatttc tgaaaattct ttacaaattt
     2101 cacctgaagc atccgtagaa ccatcattaa cgacaacaat ctcaatattt ttatatgttt
     2161 gagaaataat actatctaga catttcctca gatagttttc tacattgtat actggtacaa
     2221 tgaccgtaat tttatcatcc actagcaatt ttctccttga tttatgtgcg cgagttagtt
     2281 tagcctaact ttacaggttg tcttactctt cttcatcacg tttacggcgt ttcgcaacca
     2341 atccaatacc tgtaacagct gctagaactc caagtaacac agatccaatt gacgactcag
     2401 tacctgtatt aggcaattct ttttgggatt tacctgtcga tccagaagta tttccaactt
     2461 gtgagttcga atggtttgct gaatttgaaa ctgatgtact tgctgaagct gacgcactgg
     2521 tacttgctga agccgacgca ctcgttgatg ctgaagccga agcacttgtc gacgctgaag
     2581 ccgacgcact ggttgacgct gaagctgacg cactggtact tgctgaagcc gaagcactgg
     2641 ttgacgctga ttcagatgct gatgtgcttg ctgaagccga ggcacttgtc gatgctgagg
     2701 ctgaagcact tgttgatgct gagactgaag cactggtact tgctgaagct gacgcactgg
     2761 ttga
//
LOCUS       DBERDK010000120         2749 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.120, whole genome shotgun sequence.
ACCESSION   DBERDK010000120 DBERDK010000000
VERSION     DBERDK010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.120"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            200..373
                     /locus_tag="ACL73L_002023"
     CDS             200..373
                     /locus_tag="ACL73L_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019118552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772923.1"
                     /translation="MNKRQELIDELIKANEDGTYKTYKSTEEIKAMNNEEIQIIYSNM
                     KNYLSDKRTHINY"
     gene            559..1086
                     /locus_tag="ACL73L_002024"
     CDS             559..1086
                     /locus_tag="ACL73L_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HGS0772924.1"
                     /translation="MINEEISKEAGQAAQTIISYTIKATKESINLEKEIRKKMNETLE
                     KANGNLKSLMGDEMKIKDLYKKGQLENISIDQIDLKDLKKELNKLGVSFSVMKNKESK
                     NYEIFFQAKDIKVMEYAFKQVIAKENKKEKESILKQIKKYKDLSKNKDKTKEKGKRKV
                     KEKVKPNKKDMTREI"
     gene            1213..1917
                     /locus_tag="ACL73L_002025"
     CDS             1213..1917
                     /locus_tag="ACL73L_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HGS0772925.1"
                     /translation="MATNKRYYWIKLKEEFFTDKRIKRLRRISGGDTYTIIYLKLLLL
                     SLKDEGKLYYDGVESDFIKELALTIDETDDDVMVTVNYLINKGLLEVVTENDEYYLTE
                     IPNLIGSETAWAEKKRRYRQNKQRTLSLMSPTHVRQEIDKEREGEIDKKSTPIFYGEF
                     KNVRLSKEEYKNLKEKLNSHTDIMINKLSRYMESSGKTYQNHYVTILKWYEEDKDKLR
                     QKGLNKKMNYDVGESL"
BASE COUNT         1299 a          370 c          471 g          609 t
ORIGIN      
        1 aagctaataa aacagggaga aaaaccaagt gtaatagcac agattaaaaa gttccaagac
       61 caagtcaata aagataatga atacagagaa atgatgaaaa acaagaaaat ggatcaagaa
      121 agataagacc tggtaaaaat atcttatcca agctataata tgaccaaaca actaaagtca
      181 gatctaggag gtaatagaga tgaataaaag gcaagagcta atagatgaac tcataaaagc
      241 aaatgaagat ggaacataca aaacctataa aagcacagaa gaaataaaag caatgaacaa
      301 tgaagaaata cagattatat attccaacat gaaaaactat ctatcagaca aaagaacaca
      361 cataaactat taaaggtgga aaggtagtgt aagctatttt taaaagtata aaaggtacaa
      421 agacctaaga aatatataaa aacatcaaaa taaagtattc caccaccaag aacaataaaa
      481 taaaaacaag caaagggaag tatagaaaaa ttaaaaccta tacttccctt ttttaattca
      541 aacaaaggag agaaaagcat gataaacgaa gaaataagca aggaagcagg tcaagcagca
      601 caaaccataa tatcatacac aataaaggca acaaaagaat caatcaattt agaaaaagaa
      661 ataagaaaaa agatgaatga aactttagaa aaagcaaatg gaaacttaaa aagtcttatg
      721 ggcgatgaaa tgaaaataaa agatctctac aagaaaggac aactagaaaa tataagcata
      781 gatcaaatcg acctcaaaga cttaaaaaaa gaactaaaca aacttggagt aagtttctca
      841 gtaatgaaaa acaaagaaag caaaaactat gaaatattct tccaagccaa agacataaaa
      901 gtaatggaat atgcctttaa gcaagtcata gccaaggaaa ataaaaaaga aaaagaaagt
      961 atcctaaaac aaataaagaa atacaaagac ctatccaaaa acaaagataa gacaaaagaa
     1021 aaaggaaaaa ggaaagtaaa agaaaaagta aaaccaaaca aaaaagatat gaccagagaa
     1081 atctaaaggg agtaacgaaa agttacaccc ttaaataacc ataataacaa gacttccgaa
     1141 aaacagaagt ccagaattcc gaaaatcgga aatcaagaat ttcgattatc gaaaatcaag
     1201 aaaggagcag atatggcaac aaacaaaaga tactattgga taaaattaaa agaagaattt
     1261 ttcacagaca aaaggataaa aaggttaagg agaatatctg gaggagatac ctatacgata
     1321 atctacctaa aacttctact actaagccta aaagatgagg gcaaactcta ttatgacgga
     1381 gtagaaagtg attttataaa agagttagca ctaaccatag atgaaacaga cgatgatgta
     1441 atggtaacag taaattattt aatcaataaa ggcttattag aagttgtaac agaaaatgat
     1501 gaatactacc taactgaaat accaaactta atcggatcag aaacagcctg ggcagagaaa
     1561 aaaagaagat acagacaaaa taaacagaga acattgtcct taatgagtcc aacccatgtc
     1621 cgacaagaga tagataaaga gagagaggga gagatagata aaaagtccac ccccattttt
     1681 tatggagaat tcaaaaatgt aaggttaagc aaagaagaat ataaaaatct aaaagaaaaa
     1741 ttaaactcac acacagatat aatgataaat aaactatcca gatacatgga aagcagtggt
     1801 aagacctatc aaaaccacta tgtgacaatc ttaaaatggt atgaagaaga taaagacaaa
     1861 ctaagacaga aaggtttaaa taaaaaaatg aattatgatg taggagaatc tttatagata
     1921 aaaagaggtg gaaaggcact attaaagact ttaagtctaa aaaggtatca aagtatgcca
     1981 gagataaaaa aatataaata tgacacttta aaggcgatta gaaaattaaa gaaatgctag
     2041 aagactttta gatgacagtt accaagaggg tctaattttc actgctactt atgcagtagg
     2101 aattggacct ttttatataa gcaaattcta tttcaaaatt ggttaccaag aaagacttct
     2161 tttcactgtt aagtataaga gggtaattta tagaatttta ttacatcaag ataaatattt
     2221 tctgatggtc aaattttaaa aagattaata actctcgata aattttgaac cttaacaatt
     2281 gaatagacca agacaagacg ttaattattt ttggagcgta ataacctatc cgtcaagatc
     2341 tttcggctga ataattcggc aaaaaaagtt ctgctgagca aaaagaaagt gagcgataag
     2401 taagagtttg aatataggga gggataccct atccgctatg aagaaaatga ggataatgat
     2461 acttctaagg ttgaagccgg ctcatccgga atagaggcgg aggtgagatt cctatgttgc
     2521 taatccaaag cacttgtcaa tgtcaaaaaa cttaattaaa caaataatta ttagatataa
     2581 aactttagag gaggtatata ttggacaatg actggaatgg attggctgat ttaatagcaa
     2641 atctgattgc aaaatatgct ggtgctttag atttagataa tctacccgat ccaacgccag
     2701 caaaaaatca agagatgaaa aatagttttg acatggcaaa aacacaaat
//
LOCUS       DBERDK010000121         2679 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.121, whole genome shotgun sequence.
ACCESSION   DBERDK010000121 DBERDK010000000
VERSION     DBERDK010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.121"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            129..1622
                     /locus_tag="ACL73L_002026"
     CDS             129..1622
                     /locus_tag="ACL73L_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HGS0772926.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSV
                     ELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
                     LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPD
                     GVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKV
                     WFYDMKADGLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKEND
                     YDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK"
     gene            1635..2162
                     /locus_tag="ACL73L_002027"
                     /pseudo
     CDS             1635..2162
                     /locus_tag="ACL73L_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
     gene            2174..>2679
                     /locus_tag="ACL73L_002028"
     CDS             2174..>2679
                     /locus_tag="ACL73L_002028"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGS0772927.1"
                     /translation="MKKVKLGEVATFINGYAFKPQDWSSEGKEIIRIQNLTKTSTEIN
                     YYSGTIDKKYIVEAGDILISWSGTLGVFQWRGRSAVLNQHIFKVVFDKIDIDKSYFKY
                     VVEKGLQDAVKHTHGSTMKHLTKKYFDNIMVSYTNLGEQQRIASELDLLSKLILRRQE
                     QLEELNLLV"
BASE COUNT          945 a          386 c          537 g          811 t
ORIGIN      
        1 gtttgaagag attttcgaag agtataaaat attcaggtga cgcatagata tagttaattg
       61 aagctttgtt tgaaatctga taaaataatg atattactaa gttctaaaaa ctaaagaaaa
      121 gggaagatat gattacaggc gaattaaaaa ataaaatcga tcagctgtgg gaaattcttt
      181 ggacagaagg aaacgcaaat cctttaacaa atattgaaca gttgacttat ctcttattta
      241 tgaaagattt ggatagtgtc gagcttggac gtgaaagtga tgctgaattt ctagggattc
      301 cttatgaggg agtttttcca aaagataaac ctgaataccg ttggtcaact tttaaaaata
      361 taggagatgc tcaggaagtt tatcgtttaa tgactcagga gatttttccg tttattaaaa
      421 atctcaaggg ggatacagat gatacagcct tttcacgata tatgcgagaa gctatttttc
      481 aaataaataa acctgctacg cttcaaaagg caatttctat cttagatgtt tttccaacta
      541 ggggattaga tgtagatttt gataatgaca aacaaagtat tactgatatc ggagatatct
      601 atgaatatct gttatcaaaa ttgtcgaccg caggtaaaaa tggacagttc cgtacacctc
      661 gtcacatcat cgatatgatg gttgagttga tgcaaccgac tatcaaagat atcatctcag
      721 atcccgctat gggttctgct ggcttcttag tatctgctag ccgttactta aagcgtaaga
      781 aagatgaatg ggaaaccaat acagataata tcaatcattt tcataatcag atgtttcatg
      841 gaaatgatac ggatacgact atgttgagac ttggggcgat gaacatgatg ctacatggag
      901 tagaaaatcc acaaatcagt taccttgact cgctgtctca agataatgaa gaagccgata
      961 aatatacttt ggttttagca aatcctcctt ttaagggctc acttgactac aattcaacct
     1021 ctaatgacct tcttgcaacc gtaaaaacca aaaaaacaga attactcttt ctttctcttt
     1081 tcttgcgaac tttaaaacca ggtggacgag cagcagttat cgtacctgat ggtgtccttt
     1141 ttggttcgtc taaagctcat aaaggaattc gtcaggaaat tgtagagaat cataagcttg
     1201 atgctgtaat ctcaatgcct agtggtgtgt tcaagcctta tgctggagtt tcaactgcta
     1261 ttctcatctt tacaaaaact ggtaatggtg gtactgacaa agtctggttt tacgatatga
     1321 aagcggatgg tttaagtttg gatgataagc gacaaccgat tagcgacaat gatattccag
     1381 atattatcga acgctttcat catcttgaaa aagaagcaga acgtcagaga acggatcaat
     1441 ctttctttgt tccagttgct gagataaagg aaaatgatta tgatttgtct atcaataaat
     1501 ataaagagat tgagtatgaa aaagttgagt atgaaccaac agaggtcatc ttaaagaaaa
     1561 tcaatgattt agaaaaagaa attcaagctg gcttggctga attagaaaaa ttactcaagt
     1621 agggaggtgg ctgtatgaaa aaagtgaagt tgggggaaat cttatctcta aaaaaaggaa
     1681 agaaagccac tgtacttgct gaacaaacaa ctctaagcca acgttatatt caaatagatg
     1741 atttaagaaa taataataat ttaaaattca ctgaaagttt aaatatgact gaagcactcc
     1801 cagatgatat tctaatagca tgggatggtg ctaatgcagg aacagttggt tatggattat
     1861 cgggagctgt tggtagtaca attacggtct taaaaaagaa tgagcgatac aaagaaaaaa
     1921 ttatatcaga ttacttggga gtctttttgg aaagtaaatc gcagtattta cgagatcatt
     1981 caacaggtgc aacaattcct catttaaaca agaatatatt acttgattta caattagaat
     2041 tgctaggtat cgaagaacaa gagaacatta tctgtattct taatacgatt aaaaggctta
     2101 ttactaaaag aaaattgcag ttagatgaac taaacttgct cgtcaaatcc cgatattccg
     2161 aggaggtggt tgcatgaaga aagtgaaatt aggtgaagtg gctactttta tcaatggcta
     2221 tgcttttaaa cctcaagatt ggtcatctga agggaaagag attatcagaa tacagaatct
     2281 gactaaaact tcaacagaga taaattatta ttcgggtacg attgataaaa aatacattgt
     2341 tgaagctggt gatattctta tttcgtggtc gggtacatta ggggtttttc agtggcgtgg
     2401 aagatccgca gtgttaaatc agcacatttt taaagtggtt tttgataaga tagatataga
     2461 taaatcgtat tttaaatatg ttgttgaaaa aggtttacaa gatgctgtaa aacatactca
     2521 tggttccaca atgaaacatt taactaaaaa atattttgat aatattatgg tttcatatac
     2581 aaatttggga gagcaacaaa gaattgcaag tgagctggat ttgttaagca agctgatttt
     2641 aaggagacaa gaacaacttg aagagcttaa tctacttgt
//
LOCUS       DBERDK010000122         2640 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.122, whole genome shotgun sequence.
ACCESSION   DBERDK010000122 DBERDK010000000
VERSION     DBERDK010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.122"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            584..958
                     /gene="dhaM"
                     /locus_tag="ACL73L_002029"
     CDS             584..958
                     /gene="dhaM"
                     /locus_tag="ACL73L_002029"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002960428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HGS0772928.1"
                     /translation="MESIGLVIVSHSKHIAEGVVELISKVAKDVPITYVGGTEGGGIG
                     TSFDQVDRVVSENPADTLLAFFDLGSAKMNLKMVTDFSDKSIIINRVPIVEGAYNAAA
                     LLQAGAELSVIQTQLAELEINK"
     gene            1416..2129
                     /locus_tag="ACL73L_002030"
     CDS             1416..2129
                     /locus_tag="ACL73L_002030"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HGS0772929.1"
                     /translation="MFYKFLLFDLDHTLLDFDAAEDVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFSHFGQEKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIERDYDLYAATNGITAIQTGRLAQSGLVPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVY
                     SYQDLLDCLDKNILEKITF"
     gene            2163..>2640
                     /locus_tag="ACL73L_002031"
     CDS             2163..>2640
                     /locus_tag="ACL73L_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772930.1"
                     /translation="MTIEEYNEAVNYEFTSDTCQLANSIYQSLFKFFDKKNFSGDLIF
                     TWKSPSLVKEGDYIGRRDSQVDNLRVIGNIFPNYLTNRKYSLNMNRNGCMGDFPHDFF
                     DIYLDHVAKYAYEQKVNNIKEYYPLKRAILHQENALYFRLFSNFDDFLEKNYLKTIW"
BASE COUNT          869 a          398 c          508 g          865 t
ORIGIN      
        1 gcggaggcct tgctagaaaa agtaacagat cctagtatta gacaaaatgc tatggagaca
       61 ttgactggtc taaaaagtag tcttcttctc ggaacgaaag ataataacac tatttcagca
      121 gaagtagata gtctcttggc tttattaaag gagagtaagt aaaggtagca gcattttcta
      181 actcctaaaa acaggatagg agaacgggaa aacgaaaaat gagagcagaa tgtgagttct
      241 agttctcatt tttttcatga aaatgtgcaa aatatagtag attgaaacta gaatagtata
      301 cctctacttc taaaacattg ttagaaatcg atttgattgt cctgttctta tttcatttta
      361 ctatatctta acagatagtg taaataaaga taaactattt actggctaat taatcagtta
      421 aacactagtt aaggagtaat gatgaaaaaa agaacaatac tattattgat ggccagtctg
      481 ttagctcttg tcttaggagc atgtggtttc ttggacatat tgttcctgga tcattctcat
      541 caggattact atttactgct attttagaaa ctggggtggt ttgatggaaa gtattggtct
      601 tgttatcgtt tcacattcca aacatattgc agaaggtgtt gttgaactaa ttagtaaagt
      661 agctaaagat gttccgatta cttatgtagg aggaaccgag ggcggaggaa ttggaacgag
      721 ttttgatcaa gtagataggg ttgtttccga aaatccagca gatactttac ttgccttttt
      781 tgacctaggt tctgctaaaa tgaacttaaa aatggtgact gatttcagtg ataaaagtat
      841 catcatcaac agggttccaa ttgtagaagg tgcctataat gcagctgctc ttcttcaggc
      901 tggtgcagaa ctgtcagtta ttcaaacaca gttagcggag cttgaaatca ataaataagg
      961 aattttacta taactctttt tataggtaag ctattgatta tctcaactat aaaatgttaa
     1021 gtaaatgatt tcaaaatcag aaagggattg ctttatgcag ttccttttta ttttaacagg
     1081 agtgaaacta tagtgtttct aaattgtgaa tcaatcaaaa ctgattgtga tggggctatt
     1141 ctagctttag aatccttcaa aaattaaaat ttaaggcaat caattacttg gaagagtatg
     1201 aaagcattta gtttatagga attctaggtc tagaattaca tatgtattta tgaagacggg
     1261 gtgttcgata gttagtattg ttctattctg aaagatttga gctgtcagtt gtatagaaag
     1321 tgttcgaatt tttttaagtg attaaattag ttaattgtat gaggtgcttt atgatataat
     1381 gttcttaatg aattttcaga aaggaaaacc tcaaattgtt ctacaaattt ctactcttcg
     1441 acctcgacca cactcttctt gattttgatg ctgctgagga tgtggctttg acccaacttc
     1501 taaaagaaga aggagttgcg gatattcagg cttataaaga ttattacgtt cctatgaaca
     1561 aggctctctg gaaagacttg gagctgaaga aaatcagtaa acaagagttg gttaacacgc
     1621 gcttttctcg tttattttct cattttggac aggaaaaaga cggtagtttt cttgcccagc
     1681 gttaccaatt ttatctcgct cagcagggac aaacactatc gggcgctcat gatctcttgg
     1741 acagcctcat tgagcgtgat tatgacttgt atgctgcgac aaatggcatt actgccattc
     1801 agacaggacg tttggctcaa tctggtctag taccttattt caatcaagtc tttatctcag
     1861 aacagttgca aactcaaaag ccggatgctc tcttttatga aaagattggc cagcaaattg
     1921 ctggatttag taaagaaaag acgctgatga ttggagattc tctaaccgcc gacattcaag
     1981 gtggcaataa tgcggggatt gacactatct ggtataatcc tcatcacctc gaaaatcaca
     2041 cacaagccca gccgacttac gaagtctatt cttaccaaga cttgctggat tgtttagata
     2101 aaaatattct tgaaaagatc acattttaaa ggagacgagc taatgactac aaaaaagcta
     2161 atattactat tgaagagtac aatgaagctg ttaattatga atttacatct gacacttgtc
     2221 aattagcaaa tagtatttat caatctcttt ttaagttttt tgataagaaa aatttctctg
     2281 gcgatttaat ttttacttgg aaatctccat cattagtcaa agaaggggat tatattggga
     2341 gaagggattc acaagtagat aatcttagag taataggaaa tatatttccg aattatctta
     2401 ctaatcgaaa atatagcctc aatatgaatc gtaatggctg tatgggagat tttcctcatg
     2461 acttttttga tatataccta gatcatgtag caaaatatgc ctacgaacaa aaagttaata
     2521 atattaaaga gtattatcct ttaaaaagag cgattttaca ccaagagaat gcattgtatt
     2581 ttcgattgtt ttctaatttt gacgactttt tagaaaaaaa ttatttaaag actatatggc
//
LOCUS       DBERDK010000123         2537 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.123, whole genome shotgun sequence.
ACCESSION   DBERDK010000123 DBERDK010000000
VERSION     DBERDK010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.123"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            1..64
                     /locus_tag="ACL73L_002032"
                     /pseudo
     CDS             1..64
                     /locus_tag="ACL73L_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844241.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            44..169
                     /locus_tag="ACL73L_002033"
                     /pseudo
     CDS             44..169
                     /locus_tag="ACL73L_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(141..1616)
                     /locus_tag="ACL73L_002034"
     CDS             complement(141..1616)
                     /locus_tag="ACL73L_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HGS0772931.1"
                     /translation="MKELDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILA
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENDRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTDKLPISAMDAG
                     ADMAAVSMHKSGGSLTQSSILLIGEQMNSEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEKVIELSEYARREINAIGGYYAYSKELIDGVSVCDFDVTKLSVYTQGIG
                     LTGIEVYDLLRDEYDIQIEFGDIGNILAYISIGDRIQDIERLVGALADIKRLYSRDGK
                     DLIAGEYIQPELVLSPQEAFYSERKSLTLDDSVGQVCGEFVMCYPPGIPILAPGERIT
                     REIVDYIQFAKERGCSLQGTEDPEVNHINVIKRKTNYKKSQ"
     gene            2323..>2537
                     /locus_tag="ACL73L_002035"
     CDS             2323..>2537
                     /locus_tag="ACL73L_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGS0772932.1"
                     /translation="MQDNYTTKAKHLTIDSRRLIERWKKEGKSNREIASLLGKAPQTI
                     HTEIKRGTVRQCLGKGRFKEVYSADYA"
BASE COUNT          774 a          553 c          468 g          742 t
ORIGIN      
        1 tcctcagaaa aggcttgagg caggatttga taagctgata gaaattaagc gattgaccgc
       61 ttctaattca agggcttttt gccgtgctct aaaatcatcg ccaaatcatc aaattgaaat
      121 aagaaatagt ttagataaaa ctattgactt ttcttatagt ttgtctttct cttaataacg
      181 ttgatatgat tgacctctgg atcttccgtc ccttggaggg agcaaccacg ttccttggcg
      241 aattggatat agtcgacaat ttctcgtgta atgcgttcac caggagccaa gataggaata
      301 cctggagggt aacacataac aaattctcca cagacctgtc caacagaatc atccaaagtt
      361 aaactttttc tttctgaata gaaggcttct tgcggagaca gcactaactc gggctgaata
      421 tattctcctg ctatcaaatc ttttccatct cttgaataga gtctcttaat atcagccaga
      481 gcaccaacca agcgctcgat gtcttggatg cggtcgccga tggaaatata ggccaagata
      541 ttgccgatat caccaaactc gatctgaatg tcgtattcgt ctcgcaagag gtcataaacc
      601 tcgatacctg ttaagccaat accctgagtg taaactgaca gcttagttac gtcaaaatcg
      661 caaaccgaaa caccgtctat taactctttt gagtaggcat agtagccacc gatagcattg
      721 atttcacggc gggcatactc agatagctca atgactttct caaacgactc tttaccacga
      781 agggccaagt tgcgacgtga aatatccaaa ctagccatca acaagtaaga ggcagatgta
      841 gactgggtca ggttaattat ctgacgaacg tattcagaat tcatctgctc cccgataagt
      901 aaaatggagc tttgggtcaa actcccacca gacttatgca tggaaactgc tgccatatca
      961 gcccctgcat ccatagcaga aattggaagt ttatcagtga aatgcaaatg cgctccgtgg
     1021 gcttcatcta ctaaaaccat catgccagct tcatgagcca tttctgtcaa cccctttagg
     1081 tctgaacaga tgccgtagta agtaggattg ttgattagga tagccttagc atctggatgg
     1141 tcctttatgg cctgtgctac tcggtcattt tcaagaccta aagcgatacc aatcttagga
     1201 tctacactca tctcgatata gatgggaatg gcaccacata gaaccaacgc attgatagca
     1261 gatttatgga catttcgtgg cagaataatc ttatctcctg ccttgcaggt tgccagaatc
     1321 atagtctgca ccgatgaagt tgttccacca atcattagaa aggcatggct agctccaaaa
     1381 gcatctgcag ccagctcctc tgcatcacga ataatcgaaa taggatggcc taaattatcc
     1441 aagggtttca tcgaattgac atcaatgcct acacattttt ctcctaagag ttcgacaagt
     1501 tctggatttc cccgtccacg cttgtgacct ggaacatcaa agggaacaat ccttttcttg
     1561 cgtaacttca ccaaggcctc ataaattggg gcttggtttt gatctaactc tttcaaaaca
     1621 tactcctttc aatattttta ggaccttatg aaaactaagg aactaaaggt tgactcatga
     1681 aaaaagagca ggttttcacc tgctctgcaa tcttctgacg tgtttatttt ggtttctgtt
     1741 gagtatgtct gttcccgatg tttcctaact ctctagtagc aaatattacg tactttattg
     1801 atcgtttcac aagatgacgt gtgctttcac cacactaaaa atttatgaat tagaacaagt
     1861 gtcctaacat caacttataa aagtaggctt ttaaccctat caataatgtg gcttttcaac
     1921 cacgcttcgg cattcaaccc tgcctgtccg taggcatttt ttactcgggt ttatatttgc
     1981 tagaaaacat catctcattt tctaagcttt attactatat catgttttta agaacttgta
     2041 aagtattttt tgataaaaaa taaaaatata gtagattgaa actagaatag tacacctctg
     2101 cttctaaaac attgttagaa atagatttta ctgtcctgat cgatttgttc tgttcttgtt
     2161 tcattttact atatgttcgt aaatgaaagt tctataatga agttagccac ccagccctcc
     2221 taaaaattag atggttcaac taaccgatag ctcttgtagg cataagaaaa cacctctgta
     2281 ttatacttgt tgttcaacca caaacacaag aaaggcacag agatgcaaga caattatact
     2341 acaaaagcca aacatttgac aatcgatagc cgtcgcttaa tcgaaagatg gaaaaaagaa
     2401 gggaaatcaa atagagaaat tgcctctcta cttggaaaag ctcctcaaac tatccacact
     2461 gaaatcaagc gcgggacagt ccgacaatgt cttggaaaag ggcgcttcaa agaagtttat
     2521 tctgccgact atgctca
//
LOCUS       DBERDK010000124         2437 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.124, whole genome shotgun sequence.
ACCESSION   DBERDK010000124 DBERDK010000000
VERSION     DBERDK010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.124"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(377..1270)
                     /locus_tag="ACL73L_002036"
     CDS             complement(377..1270)
                     /locus_tag="ACL73L_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGS0772933.1"
                     /translation="MDELKREIGYKIKTIRRSCGKTQIDICGDESELTIRQLARIENG
                     QALATLPKLLLIADKLGVSLQNIVDVKVIEIPKGFLKLKDELIHSQTYADKGRIERKE
                     AILEEIYENYYENLPEEEQLIVDVTQARFDIYGSSDVTYGLGLVEEYFQQLLKKKYFS
                     VNDLLIIELYFFCCAMGLEDKEHFEELAQKVLLCSEYEDKASLVQMEKVLLSLFIQIQ
                     TEDSLIYIQTFEKIIAKTRHVFYRPHLFLLKAKYALFVDKNILEAESFYEKAISLAEL
                     LDDQVLVQRILAEKQIDFPTT"
     gene            complement(1291..1485)
                     /locus_tag="ACL73L_002037"
     CDS             complement(1291..1485)
                     /locus_tag="ACL73L_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772934.1"
                     /translation="MKKVLKYFLVFVFLFLMNIFIFKILATLGFQLIMSEKSYIVLPL
                     FSLIVLYMIDKRIRKKKKSL"
     gene            complement(1896..2021)
                     /locus_tag="ACL73L_002038"
     CDS             complement(1896..2021)
                     /locus_tag="ACL73L_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PncF family bacteriocin immunity protein"
                     /protein_id="HGS0772935.1"
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKKFLKSSEKKTEKSVKK"
     gene            complement(2024..2254)
                     /gene="blpU"
                     /locus_tag="ACL73L_002039"
     CDS             complement(2024..2254)
                     /gene="blpU"
                     /locus_tag="ACL73L_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpU"
                     /protein_id="HGS0772936.1"
                     /translation="MNTKTMSQFEIMDTEMLACVEGGGCNWGDFAKAGVGGGAARGLQ
                     LGIKTRTWQGAATGAVGGAILGGVAYAATCWW"
BASE COUNT          808 a          505 c          263 g          861 t
ORIGIN      
        1 atcaaattac ttttatatca ctaagactca ataaaagata atgtctattt aacttttata
       61 cgattactac cttcttttta gtttacactt cctacatcat aaataaacat actcaaccct
      121 cacaatctcc aaataactct tctatatcat tgaaaattga gtttcatcct ctatactaat
      181 gtaggacatt cctttaatcc aatataagaa taccttgttc cattaatctc tacccgatat
      241 tatagataga accaaattcc tatacttaaa aggacaaata acgttccaac cataataaac
      301 ctcctttctt tttgagaacg ttttctttat aggtttagta tagcaaaaac atgaaaaaaa
      361 caacatgact tagaagtcag gttgttggga aatctatctg tttttctgct aaaattctct
      421 gaactaatac ctgatcgtct agcaattctg ctagagaaat cgccttttca tagaaacttt
      481 ctgcctctaa aatattcttg tctacaaata aagcatattt tgcctttagc aaaaatagat
      541 gaggtcggta aaaaacatgt cttgttttag caataatttt ctcaaatgtt tgaatatata
      601 tcagactatc ttctgtctga atttggataa ataggctcag aagaactttt tccatttgaa
      661 ctaggctagc cttatcttca tattctgaac atagcaatac tttttgagct aactcctcaa
      721 aatgttcttt atcttctaat cccatagcac aacaaaagaa atacaattca ataatcaaca
      781 aatcatttac agaaaaatat ttctttttta ataactgctg gaaatattct tccactaacc
      841 ctaaaccgta ggtcacatct gaactaccat aaatatcaaa acgagcttgt gtcacatcca
      901 caattaactg ctcttcctca ggcaaattct cataatagtt ttcatatatt tcctcaagta
      961 tagcttcttt tcgttcgatt cttcccttat cagcgtaggt ttgagaatga atcagctcat
     1021 ctttgagttt taggaatcct tttggaatct ctataacctt aacatccaca atattctgta
     1081 atgatactcc aagtttatcc gcaatcaata ataatttagg aagtgttgct aaagcttgcc
     1141 cattttcaat acgtgctagc tgacgaatcg tcaactctga ttcatcacca caaatatcta
     1201 tctgagtttt tccacaagat cgcctaatag ttttgatttt ataaccaatt tcacgtttca
     1261 actcatccat agtaatactc cttaagttga ttacaaagat ttctttttct tcctaattct
     1321 tttgtcaatc atatataaaa cgataagcga aaacagcggt agaacaatat aactcttttc
     1381 actcattatc agctgaaatc ccagggttgc tagtatctta aagatgaaaa tattcatcaa
     1441 aaatagaaaa acaaatacta aaaaatattt taaaaccttc ttcatttctt ttcttcaact
     1501 ttcctaattt ctacacctat tttatcgaaa atatatataa aactccaaat aaacacttca
     1561 actgcacttt ttttggcaaa aaatagacag aacaaacatc ctacctacta aaaatatgat
     1621 atgaaaggaa aatgattttt tactgacacc ctaggcaagc ttgggacaaa taaatcattg
     1681 atagcaacat ttacgacatc ctatttaaag ttccttcaat ttctaatgtc cccgctattt
     1741 ctaagccacc tctggtatga tctattccta gtcagaatag ataacaccgt tctaaattac
     1801 gattcatagt tactcctttt ttgactcgtt actataagac tcctatttac atgcgcaaaa
     1861 taattttgat tcagattcat catttatagt tttgattatt ttttaacaga tttttctgtt
     1921 ttcttctccg aagactttaa aaattttttt ctaattaaat catccatata aggaccaaat
     1981 ataccaacta ctaaaccaat aataaaactt ttaaaatcca taattaccac caacatgtcg
     2041 ctgcataggc cacacctcca agtatagctc ctcccacagc accagttgct gcaccttgcc
     2101 atgttcttgt tttaattcct agctgaagac ctcgtgctgc tcctcctcca acacctgctt
     2161 tggcaaaatc tccccaattg catccgccac cttcaacgca agcaagcatc tcagtatcca
     2221 taatttcaaa ttgtgacatc gtttttgtat tcataacgaa tacctctttt ttatttttaa
     2281 tatttgtctt gttacaaact tgacaagttt agtataacag tatctattaa tttttttcat
     2341 ccaaatcttg aattggcatc gaaacgtctt gaattagctt ttttgtttca aaatcatctc
     2401 tatttttaaa aaagatgttt tctaatcact tttttac
//
LOCUS       DBERDK010000125         2416 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.125, whole genome shotgun sequence.
ACCESSION   DBERDK010000125 DBERDK010000000
VERSION     DBERDK010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.125"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..2290)
                     /locus_tag="ACL73L_002040"
     CDS             complement(<1..2290)
                     /locus_tag="ACL73L_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078495769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec-dependent serine-rich glycoprotein
                     adhesin"
                     /protein_id="HGS0772937.1"
                     /translation="MFFRRQKGEYRETDRVTRFKLIKSGKHWLRASTSLFGLFKVMRG
                     GVDTTQVMTETVEDKVSHSITGLDILKGIVAAGAVISGTVATQTKVFTNESAVLEKTV
                     EKTDALATNDTVVLGTISTSNSASSTSLSASESASTSASESASTSASTSASTSASESA
                     STSASISISASSTVVGSQTAAATEATAKKVEEDRKKPASDYVASVTNVNLQSYAKRRK
                     RSVDSIEQLLASIKAAVFSGNTTVNGAPAINASLNIAKSETKVYTGKGVDSVYHVPIY
                     YELTVINDGSKLTFTYTVTYVNPTTKTLGNISNMSNGYSIYNSGTSIQTMLTLGSDLG
                     IPSGVKNSITDKNGRQVRSYNTSTMTTQGSGYTWGNGAQMNGFFAKQGYGLTSSWTVP
                     INGTDTSFTFTPYAARTDRIGINYFNGGGKVVESSTTSQSLSQSKSLSVSASQSASAS
                     ASTSASASASTSASASASTSASESASTSASASASTSASASASTSASSSASTSASASAS
                     TSASASASTSASSSASTSASASASTSASVSASTSASASASTSASVSASTSASASASTS
                     ASESASTSASASASTSASESASTSASASASTSASASASTSASESASTSASASASTSAS
                     ASASTSASASASTSASESASTSASASASTSASASASASTSASASASTSASASASTSAS
                     ASASISASESASTSASASASTSASASASTSASASASTSASASASTSASASASTSASAS
                     ASTSASASASTSASASASTSASA"
BASE COUNT          604 a          521 c          602 g          689 t
ORIGIN      
        1 aagctgaggc gcttgtcgat gctgaggctg acgcactggt acttgctgaa gctgaggcgc
       61 ttgtcgatgc tgaggctgac gcactggttg acgctgaggc tgaggcgcta gtacttgctg
      121 aagcggacgc actcgttgac gctgaagccg acgcactggt tgacgctgag gctgaggcgc
      181 tagtacttgc tgaagcggac gcactcgttg atgccgattc agacgctgag atacttgctg
      241 aggctgacgc acttgtgctt gctgaagccg aagcactggt tgaggcggaa gctgacgctg
      301 aggtacttgc gcttgctgag gccgacgcac tcgttgatgc tgaggctgag gcgctggttg
      361 aggccgattc agaagctgag gtgcttgctg aggccgaggc gctggttgat gctgaagctg
      421 aagcactggt tgacgctgaa gccgaagcac tggttgacgc tgattcagat gctgatgtgc
      481 ttgctgaagc cgaggcactt gtcgatgctg aggctgaagc acttgttgat gctgattcag
      541 atgctgaggt acttgctgaa gctgaggcac tggttgatgc ggattcagac gcgctggtgc
      601 ttgctgaggc cgacgcactg gtgcttgctg agactgatgc actcgttgat gctgaagccg
      661 aggcgctggt gcttgctgag actgatgcac tcgttgatgc tgaagccgag gcgctggtgc
      721 ttgctgaaga cgacgcactg gtacttgctg aagctgacgc actggttgac gctgaagctg
      781 aggcactggt tgatgctgaa gacgacgcac tcgttgatgc tgaagccgac gcactcgttg
      841 atgctgaggc tgaggcagac gttgatgcgg attcagacgc acttgttgat gctgaagctg
      901 aggcactcgt tgatgctgaa gccgacgcac ttgttgatgc tgaagctgag gcgctttgac
      961 tagcacttac tgagagtgac ttagactgtg aaagtgactg actggtcgtg ctagattcaa
     1021 ctacctttcc tccaccattg aagtagttaa ttccaattct atctgttcta gcagcgtaag
     1081 gggtaaatgt aaaggatgta tccgttccat taattggtac agtccaagat gatgttaatc
     1141 catatccttg cttagcaaag aaaccattca tttgggcacc atttccccaa gtatacccac
     1201 taccctgcgt cgtcattgta gatgtattat aggatcgaac ctgtctacca tttttgtcag
     1261 taatggagtt ctttacacct gaagggatac caagatcact gccaagggtt aacattgttt
     1321 gtattgaagt acctgaatta tagatagaat atccattact catatttgat atattaccaa
     1381 gagtctttgt tgtaggattc acatacgtaa ccgtataggt aaaggtcaat tttgaaccat
     1441 catttatcac tgtcaattca tagtaaattg gaacatgata taccgaatct acacctttac
     1501 ctgtataaac ttttgtctca cttttagcaa tgtttagact tgcattaatt gcaggggcgc
     1561 catttacagt cgtattgcca gaaaaaacag cagcttttat agaagccagc aattgctcga
     1621 tggaatccac tgaacgcttg cgtcgcttag cataagattg gagattgaca tttgtaactg
     1681 atgctacata atcactagct ggtttcttac gatcttcttc gaccttctta gcagttgctt
     1741 ctgtagcggc agctgtttgt gatcctacca cagtagatga tgcagagata cttatcgaag
     1801 ccgatgtact tgctgattct gatgcacttg tactcgctga ggtcgaagcg ctggttgagg
     1861 ctgactcaga tgcagatgta cttgccgact ctgaagctga caaactagtt gaactcgctg
     1921 aattacttgt agatatcgta cctagaacta ctgtatcatt tgttgccaaa gcatccgttt
     1981 tctctacagt tttttcaagt actgccgact catttgtaaa taccttcgtt tgagttgcaa
     2041 cggttccact tatgacagct cccgcagcaa ctatcccctt gaggatatca agcccagtaa
     2101 ttgaatgact tactttatct tctaccgttt cggtcatgac ctgagtagta tcaacaccac
     2161 cacgcatgac cttaaacaag ccaaaaagag aagtcgaggc ccgtagccaa tgcttccctg
     2221 atttgattaa tttgaaccga gttactcggt ctgtttctct atattcaccc ttctgacgtc
     2281 taaaaaatat tttgaaattt cctttcgagt attattttca tgtatggata taaataaatt
     2341 accatactat aattatagaa tagggaaccc aaaatagcaa gaaacttgag gtatttttgc
     2401 taaaaaaaaa aagcat
//
LOCUS       DBERDK010000126         2405 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.126, whole genome shotgun sequence.
ACCESSION   DBERDK010000126 DBERDK010000000
VERSION     DBERDK010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.126"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..154
                     /locus_tag="ACL73L_002041"
     CDS             <1..154
                     /locus_tag="ACL73L_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGS0772938.1"
                     /translation="KKILLPLPPLALQNEFADFVALVDKSQLAIQKSLEELETLKKSL
                     MQEYFG"
     gene            214..450
                     /locus_tag="ACL73L_002042"
     CDS             214..450
                     /locus_tag="ACL73L_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HGS0772939.1"
                     /translation="MVVKTRKQGNSITITIPSEFNIPSGVKYEAKLLPSGEIIFTPEE
                     LGQQVSYVSDDAFDLNLDKIFDEYDDVFKALVEK"
     gene            447..860
                     /locus_tag="ACL73L_002043"
     CDS             447..860
                     /locus_tag="ACL73L_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HGS0772940.1"
                     /translation="MTIYLTEKQIEKINALAIQRYSPNEKIQTVSSSALNMIVNLPEQ
                     FVFGKSLYPTIFDKATILFVQLIKKHVFANANKRTAFFVLVKFLQLNGYRFSVTVEEA
                     VKMCVTIAVEALTDEKMTSYSKWVSEHSVREKVKK"
     gene            978..1943
                     /gene="xerA"
                     /locus_tag="ACL73L_002044"
     CDS             978..1943
                     /gene="xerA"
                     /locus_tag="ACL73L_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase/integron
                     integrase"
                     /protein_id="HGS0772941.1"
                     /translation="MESKVTIIMQEMLPLLNNEQLLALRESLEHHLVDGKKQQKYSNN
                     NLLQLFITAKQVEGCSSKTIRYYQRTIENLFNAIKESVTQLTTDDLRSYLANYQSEKD
                     CSKANLDNIRRILSSFFAWLEQEEYIIKNPIRRIKKIKTEQNVKETYTDEHLEIMRDN
                     CENLRDLAIIDLLASTGMRVGELVQLNRSDIDFENRECVVFGKGKKERPVYFDARTKI
                     HLRNYLNDRKDSHPALFVTLVGKAQRLGIAGVEIRLRKLGDKLGIQKVHPHKFRRTLA
                     TKAIDKGMPIEQVQKLLGHSKIDTTLAYAMVNQNNVKHSHQKFIS"
     gene            1948..2405
                     /locus_tag="ACL73L_002045"
                     /pseudo
     CDS             1948..2405
                     /locus_tag="ACL73L_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT          885 a          362 c          454 g          704 t
ORIGIN      
        1 aaaaaaaata cttctccctc tcccccctct agccctccaa aatgagttcg cagactttgt
       61 agccctggtc gacaaatcac aattggcaat ccaaaaatct ctggaagaac ttgaaacttt
      121 gaagaaatct ctgatgcagg agtattttgg ctgatattct gccattgtaa ttacggtaat
      181 gatttgttat aatacttcaa aggaggaaat cagatggtag taaaaacaag aaaacaagga
      241 aattcaatca ccattacgat tccaagtgaa tttaatattc caagtggtgt taaatacgaa
      301 gcgaaattgt taccaagtgg tgagattatc tttactcctg aagaattggg gcagcaggtt
      361 tcttatgtat ctgatgatgc ctttgactta aatttagata aaatatttga cgaatacgac
      421 gatgttttca aagctttggt ggaaaaatga caatctatct gacagaaaag caaattgaaa
      481 aaataaatgc tttagcaatt caacggtatt ctccaaatga gaaaattcaa acagttagtt
      541 cttctgcctt aaatatgatt gtgaacttac cagaacaatt tgtctttggg aagtctcttt
      601 atccaacaat ttttgataaa gcaacgatac tatttgtcca attgataaag aagcatgttt
      661 ttgctaatgc taataaaaga actgctttct tcgttttggt caaattttta caattaaacg
      721 gctatcgttt ttctgtaacg gtagaagaag cagtaaaaat gtgtgtaacc atcgcagtag
      781 aagctttaac tgatgaaaaa atgacaagct actccaaatg ggtttctgaa cattctgtta
      841 gagaaaaggt caaaaagtaa cctagtatgc tggatttgaa tgagcacaag aaaataaatg
      901 aacagacaat attagaattc tgtaatgcag aaactgatat tgtctctttt tattgatgaa
      961 taagaaagtg agaaattatg gaatcaaaag ttacaattat catgcaagaa atgttacctc
     1021 ttttaaataa tgaacaatta ctagcgttga gagagagttt agaacatcat ctagtagacg
     1081 gaaaaaagca gcagaagtat tcgaataata acctgttgca actatttatt accgccaagc
     1141 aggtagaggg ctgtagctca aaaacaattc gttattacca gaggacgatt gaaaacttgt
     1201 ttaatgctat taaagagtct gtgacacaac tcacaacaga tgatttaagg agttatttag
     1261 caaattacca gtctgaaaag gattgtagta aggcaaattt agacaatatt aggcgtatat
     1321 tgtcttcttt ttttgcttgg cttgagcaag aggaatatat cattaaaaat cccattcgac
     1381 ggataaagaa aattaagact gagcaaaatg tgaaggaaac ttatactgat gaacatttgg
     1441 aaattatgcg tgataactgt gaaaatttga gagatttggc aataatagac ctactagcat
     1501 cgacaggtat gcgtgtaggg gagcttgtac agttgaatcg ttcagatatt gattttgaaa
     1561 acagagagtg tgttgtcttt ggtaaaggaa agaaggagag accagtatat tttgacgctc
     1621 ggacgaaaat tcatttaaga aattatctta acgacagaaa agatagtcac cctgctcttt
     1681 ttgtaacgct agttggaaaa gcccagagac ttggaattgc tggtgtagag attcgcttaa
     1741 gaaagttagg agacaaactc ggcatacaaa aggttcaccc acataagttc agaagaactt
     1801 tagcgactaa ggcaattgat aaaggtatgc ctatcgaaca agtccaaaaa ctgctaggtc
     1861 atagcaagat tgacacaacc ctggcctatg ccatggtcaa tcaaaataat gtcaagcatt
     1921 cacaccaaaa attcatctct taaaagcaaa tcccgattta acgagatgtt tggggaaaat
     1981 aaaatatttg aaagcattga taacttattt gatattatag atggtgatag gggcaaaaat
     2041 tatcctaaat cagatgagtt gtttagtgag gagtactgtt tatttttaaa tacaaagaat
     2101 gttactaaaa acggattttc attcgataca aagcaattta tcactaaaac aaaggataaa
     2161 ttacttcgaa aaggcaaact tgagcgttat gatatagtct tgacaacaag aggtactgtt
     2221 ggaaatgtag cgtactacga tgaattaata aaatataaac atttacgtat aaattcaggt
     2281 atggtaatat tacgtcccaa gacaccaaat ctaaatcaga aatttattat ccatgtttta
     2341 aggaataata attatagtcg agtgatatca ggaagtgctc agcctcagtt accaattaca
     2401 aaatt
//
LOCUS       DBERDK010000127         2377 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.127, whole genome shotgun sequence.
ACCESSION   DBERDK010000127 DBERDK010000000
VERSION     DBERDK010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.127"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..188)
                     /locus_tag="ACL73L_002046"
     CDS             complement(<1..188)
                     /locus_tag="ACL73L_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HGS0772942.1"
                     /translation="MIGSIAAILTTFAFLPQVFRVVKTKDTGSIALGMYVMQVIGIAL
                     WLAHGIRIGDLPLILANSV"
     gene            complement(240..1676)
                     /locus_tag="ACL73L_002047"
     CDS             complement(240..1676)
                     /locus_tag="ACL73L_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HGS0772943.1"
                     /translation="MTTFKDGFLWGGAVAAHQLEGGWQEGGKGISVADVMTAGRHGVA
                     REITLGVLEGKYYPNHEAIDFYHRYKEDIALFAEMGFKCFRTSLAWTRIFPKGDELEP
                     NEEGLQFYDNLFDECLKNGIEPVITLSHFEMPYHLVTEYGGWKNRKLIDFFARFAEVV
                     FKRYKDKVKYWMTFNEINNQANYQEDFAPFTNSGIVYEEGDNREAIMYQAAHYELVAS
                     ARAVKIGHEINPDFQIGCMIAMCPIYPVTCNPKDILMAMKAMQKRYYFADVHVLGKYP
                     EHIFKYWERKGISVDFTDQDKEDLLGGTVDYIGFSYYMSFAIDSHRENNPYFDYLETE
                     DLVKNNYVKASEWKWQIDPEGLRYALNWFTDHYHLPLFIVENGFGAIDQVAADGMIHD
                     DYRIEYLGAHIREMKKAVVEDGVDLMGYTPWGCIDLVSAGTGEMRKRYGFIYVDKDDN
                     GKGSYNRSPKKSFGWYKEVISSNGESVE"
     gene            complement(1866..2204)
                     /locus_tag="ACL73L_002048"
     CDS             complement(1866..2204)
                     /locus_tag="ACL73L_002048"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HGS0772944.1"
                     /translation="MTLTIHVYYKGDGDSAIKFAREMLDSGLVEEIRNQKGNLKYEYF
                     LPIEKEGTILLIDQWINQEALDEHHQSKTMQKILDLRKKYHLQMQVERYIEDDSGMPE
                     SDKSFIDTGN"
BASE COUNT          797 a          510 c          288 g          782 t
ORIGIN      
        1 acactatttg ctaaaatcaa aggcaagtct cctatacgaa taccatgagc aagccacaat
       61 gcaataccaa taacttgcat aacatacata cctagagcaa tagatcctgt gtcctttgtc
      121 ttaactacac gaaaaacttg tggtaaaaat gcaaatgttg ttaaaattgc tgcaatactt
      181 ccaatcatat gtcaccccaa tatgctaaac aaactgagaa taatctcagt ttgtttatac
      241 tattctactg attcaccgtt agatgaaata acttccttat accagccaaa agattttttc
      301 ggggaacgat tataacttcc cttcccatta tcatctttat ctacataaat aaagccataa
      361 cgtttccgca tttcaccggt accagctgaa accaaatcaa tacatcccca tggagtataa
      421 cccattaaat caacaccatc ttcaactaca gcctttttca tttcacgaat atgggcacct
      481 agatattcaa ttctataatc atcatgtatc ataccatctg ctgcaacttg atctatagct
      541 ccaaaaccat tttcaacaat aaagagtggt aagtgatagt ggtctgtaaa ccaatttaac
      601 gcataacgca aaccttctgg atcaatttgc cacttccatt cagaagcctt aacataatta
      661 tttttcacta aatcttctgt ttcaagataa tcaaaataag gattattttc acgatgagag
      721 tctatagcaa aggacatata gtaactgaaa ccaatgtaat ctacagtccc accaagtaaa
      781 tcttctttat cctggtcagt aaaatcaact gaaatacctt ttcgttccca atacttgaaa
      841 atatgctcag gatatttacc taaaacatgc acatcagcaa aataataacg cttctgcata
      901 gctttcattg ccattaagat atccttagga ttgcaagtaa ctggataaat tggacacatc
      961 gcaatcatac aacctatttg aaaatctgga ttaatctcat gaccaatttt tacagctcgt
     1021 gcagaagcaa ctaattcgta atgtgctgct tgatacataa ttgcctctct attatcacct
     1081 tcctcatata caatacctga gttagtaaat ggtgcaaaat cttcctgata attcgcttga
     1141 ttattgattt cattgaaagt catccaatat ttaaccttat ctttgtaacg tttaaatacg
     1201 acttctgcaa aacgagcaaa gaaatcaatc aatttcctat ttttccaacc accatattcg
     1261 gtcactaagt gataaggcat ttcaaaatga gatagagtga tgacaggttc aataccattc
     1321 tttaagcatt catcaaaaag attatcataa aactgtaatc cttcttcatt cggctctaac
     1381 tcatcacctt ttggaaagat acgtgtccat gcaagagagg tacggaagca cttgaatccc
     1441 atttcagcaa aaagtgctat gtcttcttta taacgatgat aaaaatctat cgcctcatga
     1501 tttggataat atttaccctc taaaactccc aaagtaattt cacgagctac tccatgacga
     1561 ccagcagtca taacatcagc aacactaatt cccttgccac cttcttgcca tccaccttca
     1621 agttgatgag cagcaacagc accaccccat aaaaatccat ctttaaaagt agtcatcttt
     1681 tttcctcctg actttgatac tcttattata aacctcaaat caaaagatga aaacgcattc
     1741 tttttcctta ttgttaagga aagaagtaat ttttaatgga aatagaacaa tatcttcttg
     1801 tattctcgta atgatatctt tacgattttc aatactttca aactacaaaa actctcacaa
     1861 taattctaat tccctgtgtc tataaacgac ttatcgcttt ctggcatccc agaatcatct
     1921 tctatataac gttcaacttg catctgcaag tgatattttt ttcttaaatc taagattttc
     1981 tgcattgtct ttgattgatg atgttcatct aaagcttctt gatttatcca ctgatcaata
     2041 aggagaatag ttccctcttt ttcaattggt aaaaaatatt cgtatttcaa gttacctttt
     2101 tgatttctaa tttcttcaac aaggccacta tcaagcattt ctcttgcaaa ctttattgca
     2161 ctatctccat cacctttata atatacatga atagtcaatg tcatcttata tcctccaaaa
     2221 tcatccttca attttaaaaa aacaagttta gatgaggatc taaacttgtt ttttatgaac
     2281 taattatcta acgtttcgcc attactttca atcacttctt tataccaata aaatgatttt
     2341 ttcttatagc gatttatagt caattgaaac aagagca
//
LOCUS       DBERDK010000128         2358 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.128, whole genome shotgun sequence.
ACCESSION   DBERDK010000128 DBERDK010000000
VERSION     DBERDK010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.128"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(179..2161)
                     /locus_tag="ACL73L_002049"
     CDS             complement(179..2161)
                     /locus_tag="ACL73L_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HGS0772945.1"
                     /translation="MKSSKLLALAGVTLLAATTLAACSGSGSSAKGEKTFSYIYETDP
                     DNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYTIRKDA
                     KWYTSEGEEYAAVKAQDFVTGLKYAADKKSDALYLVQESIKGLDAYVKGEIKDFSQVG
                     IKALDEQTVQYTLNKPESFWNSKTTMGVLAPVNEEFLNSKGDDFAKATDPSSLLYNGP
                     YLLKSIVTKSSVEFAKNPNYWDKDNVHIDKVKLSFWDGQDTSKPAENFKDGSLTAARL
                     YPTSASFAELEKSMKDNIVYTQQDSITYLVGTNIDRQSYKYTSKTSDEQKASTKKALL
                     NKDFRQAIAFGFDRTAYASQLNGQTGASKILRNLFVPPTFVQADGKNFGDMVKEKLVT
                     YGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVTFPIHLDMPVDQTATTKVQ
                     RVQSMKQSLEATLGADNVIIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFA
                     DPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVGLYDYEKLVTEAGDEATDVAKRY
                     DKYAAAQAWLTDSALIIPTTSRTGRPILSKMVPFTIPFALSGNKGTSEPILYKYLELQ
                     DKAVTVDEYQKAQEKWMKEKEEFNKKAQEDLAKHVK"
BASE COUNT          672 a          486 c          414 g          786 t
ORIGIN      
        1 caaataatag ctgagaaagc aataggaaac aagtaaaatt aacaaaaatt ttctctattc
       61 tgacaaaaag aggctccaac ttttgggggt cagtacattt agaatctttc tttacaaatg
      121 taaaaaagga ttcagcattc aagagaaata ctaaatcctt tcttatattt tgcaacagtt
      181 atttcacatg ttttgcgaga tcttcttgag cctttttatt aaactcttct ttttctttca
      241 tccatttttc ctgagctttt tggtattcat ctacagtgac tgccttgtct tgaagttcca
      301 agtatttata taagattggt tcacttgtac ctttatttcc tgacaatgca aatggtattg
      361 taaatggtac catcttagac aagattggac gccctgtacg agatgtagtt ggaataatca
      421 aagcactatc tgtcaaccaa gcttgggctg cagcgtattt atcatagcgt ttagcaacat
      481 ctgtagcctc atcaccagcc tcagtaacca atttttcgta gtcatataga cctacttttt
      541 tagcagctac attatcttcc cctgagtcaa accctaaata tgttttagta ctttctccta
      601 cagatggttt gatgatatca aggtaggttg atggatcggc aaagtctgga ccccaaccga
      661 cattatctga taaatcccag tcttcgccag cagcattttc agcaaaatat gtaatattgt
      721 ttacttcgtc tttttgtagt tgttggatat caataatgac attatcagct cctaaagttg
      781 cttccaagga ttgtttcata gattggacgc gctgaacttt tgtagttgct gtttggtcaa
      841 ctggcatatc caaatgaatt gggaatgtca caccttctgc ttgtaaggct gatttagctt
      901 tagcaaattc agccttggct ttttctggat tgtaaagacc atcctgagaa tctgcaagat
      961 taacatcctt ccattcatcc ccataagtga ccaatttctc tttgaccata tcgccaaagt
     1021 ttttaccatc tgcttgaaca aatgttggtg gcacaaagag attacgcaag attttgcttg
     1081 ctccagtttg tccattcaac tgagaggcat aggctgtacg atcaaaacca aaggcaatag
     1141 cctgacggaa atccttgttt aagagagcct ttttagtcga tgccttttgt tcgtcgctgg
     1201 tcttagatgt gtatttatag gactgacggt caatatttgt accaactaga tacgtaatag
     1261 agtcttgttg agtatagaca atattgtcct tcatactctt ctcaagctct gcgaaacttg
     1321 cacttgttgg atagagacga gctgctgtaa ggctaccatc tttaaagttt tctgcaggtt
     1381 tgctggtatc ttgaccatcc cagaatgaca atttaacttt gtcaatatgc acattgtcct
     1441 tatcccagta gttcggattt ttcgcaaatt caacagaaga tttggtcaca atggatttca
     1501 acaaataagg accgttatac aagagactac ttggatccgt agctttggca aaatcatccc
     1561 cttttgaatt caaaaactct tcattaactg gcgcaagcac acccatggtt gtcttagaat
     1621 tccagaagct ttctggtttg ttcaaagtgt actgaactgt ctgttcatcc agagccttaa
     1681 ttcctacttg tgagaaatct ttgatttccc cttttacata ggcatccaac cctttgattg
     1741 attcttgaac aaggtaaaga gcatctgatt ttttatcagc agcatatttt agtcctgtta
     1801 caaagtcttg agctttgact gccgcgtatt cttcaccttc agaagtatac cattttgcat
     1861 ccttacggat agtataagtg taagtcaatc catccttgga tacagaccaa tcctcagcca
     1921 tagacggcac aaagttcccg tagcgatcat tttctagcaa accatcaacc acgttactgg
     1981 taatatttgc tgtcgcagcc ttagcagttg tcaaatagtt gaggttatca gggtctgtct
     2041 cataaatgta tgagaatgtc ttctcacctt tagcgcttga acctgatcca gagcatgcag
     2101 ctaaagtagt cgccgccaat aatgtcacgc ccgcaagggc aagtagtttt gaacttttca
     2161 tcattttctc ctttaaaact ttctctccat tatagactct tttcagaaag ttgtcaacag
     2221 aattttcaga atttttgaaa attatttttc aaacaacatc tttgcaaaaa atatgaatat
     2281 cgtaaccgtc caataacgaa gtatattgaa aaatctccag actagagaac tcacggatag
     2341 ttcctaatct ggagattt
//
LOCUS       DBERDK010000129         2245 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.129, whole genome shotgun sequence.
ACCESSION   DBERDK010000129 DBERDK010000000
VERSION     DBERDK010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.129"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            145..1635
                     /gene="mgaSpn"
                     /locus_tag="ACL73L_002050"
                     /gene_synonym="mgrA"
     CDS             145..1635
                     /gene="mgaSpn"
                     /locus_tag="ACL73L_002050"
                     /gene_synonym="mgrA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor transcriptional regulator
                     MgaSpn"
                     /protein_id="HGS0772946.1"
                     /translation="MRDLLSKKSHRQLELLELLELLFEHKRWFHRSELAELLNCTERA
                     VKDDLSHVKSAFPDLIFHSSTNGIRIINTDDSDIEMVYHHFFKHSTHFSILEFIFFNE
                     GCQAESICKEFYISSSSLYRIISQINKVIKRQFQFEVSLTPVQIIGNERDIRYFFAQY
                     FSEKYYFLEWPFENFSSEPLSQLLELVYKETSFPMNLSTHRMLKLLLVTNLYRIKFGH
                     FMEIDKDSFNDQSLDFLMQAEGIEGVAQSFESEYNISLDEEVVCQLFVSYFQKMFFID
                     ESLFMKCVKKDSYVEKSYHLLSDFIDQISVKYQIEMENKDNLIWHLHNTAHLYRQELF
                     TEFILFDQKGNTIRNFQNIFPKFVSDIKKELSHYLETLEVCSSSMMVNHLSYTFITHT
                     KHLVINLLQNQPKLKVLVMSNFDQYHAKFVAETLSYYCSNNFELEVWTELELSKESLE
                     DSSYDIIISNFIIPPIENKRLIYSNNINTVSLIYLLNAMMFIRLDE"
     gene            1997..>2245
                     /locus_tag="ACL73L_002051"
     CDS             1997..>2245
                     /locus_tag="ACL73L_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGS0772947.1"
                     /translation="MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLRKVQR
                     AIKTLDYIPDNYARGMKKNRTETIALIIPSIWHPFFSEF"
BASE COUNT          786 a          299 c          369 g          791 t
ORIGIN      
        1 aaaattgttt atatattgtc tccgtagtgt tattatacga aataaaagat tttaggaaaa
       61 gtcgttatat catgctatac ctattctttg tggtataatt gcaagaggtt taatccgata
      121 atttataaag agagaaagat attcatgaga gatttattat ctaaaaaaag tcataggcaa
      181 ttagaattat tagaattatt agaattatta tttgaacata aacgttggtt tcatcgttct
      241 gaactagcag agttattaaa ttgtacagaa cgtgcagtca aagatgatct atcccatgtt
      301 aaatctgctt ttcctgactt gatttttcat tcttctacta atggtatacg cattattaat
      361 accgatgata gtgatattga aatggtttac catcatttct ttaaacattc aactcatttt
      421 tcgattttag aattcatctt ctttaatgaa ggttgtcaag ctgagagtat ttgtaaagaa
      481 ttttatatca gttcatcttc gctctatcgt attattagcc aaatcaataa agtgattaaa
      541 aggcaatttc aatttgaagt cagtctgacc cctgttcaaa tcattggaaa tgagagagac
      601 attcgttact tttttgcaca atatttttca gaaaaatatt atttcctaga atggccattt
      661 gaaaattttt catcagagcc actatctcaa ttgttagaat tggtttataa ggaaacaagc
      721 tttccaatga atttgtcaac gcatagaatg ctaaagttgc tcctagttac gaacctatat
      781 agaataaagt ttggtcattt tatggaaata gataaagatt cttttaacga ccaaagtttg
      841 gattttttaa tgcaggcaga aggaatagaa ggtgttgctc agagttttga atcagaatac
      901 aatatctctt tagatgaaga agttgtttgc caattatttg tgtcttactt tcaaaaaatg
      961 tttttcatag atgaaagtct ctttatgaaa tgcgtaaaaa aggatagtta tgttgaaaaa
     1021 tcttaccatc tattgagtga ttttattgat cagatttcag tcaagtatca gattgaaatg
     1081 gagaataagg ataatctgat ttggcatctg cataataccg cacatctgta tcgtcaagag
     1141 ttgtttactg agtttatttt atttgatcaa aaagggaata caatcaggaa ctttcaaaat
     1201 atttttccaa aatttgtttc agatataaaa aaagagcttt ctcactattt agagactctt
     1261 gaggtttgtt ctagttcaat gatggtaaat catctatctt ataccttcat cactcatacc
     1321 aaacatttgg tgattaattt gctacaaaat cagcctaaac taaaggtttt agttatgagt
     1381 aattttgatc agtatcatgc aaaattcgtt gcagagacac tttcttatta ctgtagcaat
     1441 aattttgaac tggaagtttg gactgaatta gaattatcaa aggaatcttt agaagattca
     1501 tcttatgata tcattatttc taattttatt attcctccta ttgaaaataa gagactcatc
     1561 tattcaaata atataaacac ggtctcactc atatatttgt taaatgccat gatgtttatt
     1621 cgattagatg agtaaccaaa aataacaaaa aaacgctatt tcgcgtttga actgtacctc
     1681 aaaagttaga cagaaaaaat ctaatttttg gggtgttttt gttaagaaaa gaccttcaat
     1741 catttaagca aaatgtacat ctagatttat agccctctaa aactcttgct ttttttgtag
     1801 aagatacgaa aaaagccata aaaccaaggc ttttcctgtt ttatgtagat gtttacacat
     1861 gaaaattgaa gtattttcaa aagctaattg gagtgatttt attcatgatg ggataaatga
     1921 tctggccaaa aatgattgag aaatagttgc ttaaaaagca tttgcatgat actatatatt
     1981 gaaaggaggt gataatatgg tagaacaaag aaaatcaatt accatgaaag atgttgcttt
     2041 agaagcagga gttagtgttg gaactgtttc acgtgtaatt aataaagaaa aaggcattaa
     2101 agaagtaact ttaaggaaag ttcaaagggc tataaaaaca ttggactata tccctgataa
     2161 ttatgctcga ggtatgaaaa agaatagaac agaaactatc gctttgatta ttccgagcat
     2221 atggcatcca tttttttcgg agttt
//
LOCUS       DBERDK010000130         2116 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.130, whole genome shotgun sequence.
ACCESSION   DBERDK010000130 DBERDK010000000
VERSION     DBERDK010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.130"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(93..945)
                     /locus_tag="ACL73L_002052"
                     /pseudo
     CDS             complement(93..945)
                     /locus_tag="ACL73L_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182753.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rgg/GadR/MutR family transcriptional regulator"
     gene            936..1001
                     /locus_tag="ACL73L_002053"
     CDS             936..1001
                     /locus_tag="ACL73L_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHP2/SHP3 family peptide pheromone"
                     /protein_id="HGS0772948.1"
                     /translation="MISKFFPILMLVMDIIIIVGG"
     gene            1105..1974
                     /locus_tag="ACL73L_002054"
     CDS             1105..1974
                     /locus_tag="ACL73L_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HGS0772949.1"
                     /translation="MKHKKVLIIGYGIMTRGILKILKQSKKYNIYLYSRHLNSTINGI
                     IFIREDELPDAINQVDFIITCFSNHIETDNFINEFLLNIQSLRKTIVIDFTTSRIDCV
                     EKFKKTVEIKGGYYIEAPFTGGKLGSNSGQLSIFLHIDEGVNYNKKELMELLKLISKK
                     IYFFEKSTEPTKFKLIYNSWGAYLLYSIKLFNPLQYDFSPKSLEVANTIVCNDGWMSL
                     VCNSKLEQINDQDFDDIHFRVEYMVKDLEYANFSVFHDEVKEINYLLQQYGDCLVTDS
                     GKDYTVIGKYDNL"
BASE COUNT          754 a          257 c          356 g          749 t
ORIGIN      
        1 atctgtctat agttaaaact acaagttcat cggagttgat aaggttttat tgagactctt
       61 gattataaaa cgttagatgg taaggaatca tttcatttat tgtttacaag tttatcaaaa
      121 tggctagtat agtgtaattt taacttgtct tctcctaaaa tatcgagaac ttgtatagct
      181 tgtttcattg tttcaatacc actacttaat tgacgtttaa attcatagta accggttgca
      241 tagagaaaaa ctgtctgttc ataaaagttt aattcattct ctgaacaatc cctttatctc
      301 attaattaaa taagagcaat tagaaaattc ttctttatct atgctgagaa tgaaacaatt
      361 tatagctagc tgagtgacaa tccgcttatt tgttttattt agagatgagt aaatataatt
      421 atttaacatt tctttagtta ataagaagta ggagttgtag ttaattgttc gtacacagtt
      481 acctaataaa ataatctcat aataacccca tttttcaact ttaaagagat aatctgctag
      541 ttgaagtaat tcatcatctg atggaatgat tcttgaatcc attgtgtgta aaatggattt
      601 taccattgtt ttttcaaaag aatctaaagt gtaatttgag gaatccgaaa gtagagattg
      661 tatattattg atgttttgta atgaatattg tttacggata tactgtatta agttggcaaa
      721 tgactcagtt tttgtatagt cttcatcgtg taaaataaga aactcatcca aagtaatgtg
      781 taatttgtct aaaatattga tcagtcggat acatgatatc tctgactctc cacgttcaaa
      841 tcgggatatt tgcgattttg aaagatgctc atcagcaaca gagcatagac tgatttgttt
      901 gcctttacga acttttctaa gtgtgatgcc aagttttgat ttcaaaattt ttcccaattt
      961 tgatgctagt gatggacatt atcattattg ttggtggtta aatcacttag gtgtacacct
     1021 aagtctttag attatctaaa gactatactt actagctaaa acatatttaa gttaaatcgt
     1081 aaaattttag agagtggtga gactttgaaa cataaaaaag tattaattat agggtatggt
     1141 ataatgactc ggggtatatt aaaaatcctg aaacagtcga aaaaatataa tatataccta
     1201 tatagtaggc atctaaattc aacaataaat ggaataatat ttattagaga agatgaatta
     1261 cctgatgcta taaatcaggt tgattttata atcacttgtt ttagtaatca tattgaaacg
     1321 gataatttca ttaatgagtt tttattaaat atacaatcac ttcgtaaaac catagtaatt
     1381 gattttacaa cctccaggat tgattgcgtt gaaaaattta aaaaaactgt tgaaattaaa
     1441 gggggatact atatagaggc tcctttcaca ggaggtaagt taggaagtaa tagtggtcaa
     1501 ctatcaattt ttttacatat tgatgaaggt gtaaactata ataaaaagga gttaatggaa
     1561 ttattaaaat taatttcaaa aaaaatatac ttttttgaaa agagtacgga accaacaaaa
     1621 ttcaagttaa tttataactc gtggggagcg tatttgcttt actctattaa gctattcaac
     1681 cctttacagt atgatttttc accaaagtct ctagaggtag cgaatacaat cgtatgtaat
     1741 gatggttgga tgagtttagt atgcaactcc aaattagaac agatcaatga tcaagatttt
     1801 gatgacatac actttagagt agaatatatg gtaaaagatt tagaatatgc taatttttca
     1861 gtctttcatg atgaagtgaa agaaataaat tatttattac agcaatatgg ggattgctta
     1921 gtaactgata gtggaaaaga ttatacggta ataggaaagt atgataactt ataggaatga
     1981 cttgctgtct aaagaattag atatactaat ttcatttttt aaaaaatgcg aagtaggaaa
     2041 aatttcactc gttattactg gttccttggc aagaggtaat ccaagaatca aggatgggaa
     2101 attagaatcg gatatt
//
LOCUS       DBERDK010000131         2114 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.131, whole genome shotgun sequence.
ACCESSION   DBERDK010000131 DBERDK010000000
VERSION     DBERDK010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.131"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..>2114)
                     /locus_tag="ACL73L_002055"
     CDS             complement(<1..>2114)
                     /locus_tag="ACL73L_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S74"
                     /protein_id="HGS0772950.1"
                     /translation="RYVVHIPKGWTNESKQTTNEWLFNFNQEGTVWIWMPKFEISDVD
                     TSYSEAPEDIEGQISTVESTFKQRANSLEAGVNRLTEGLRTKADISSLNVTAENIRQS
                     VKSLETDTQNKLNQKLSQAEFEVRAGSIRQEILNATKDKASKSELTQTAEELASRIAS
                     VHLGRRNLLKGTKELARYKPVSEYNGFKVIRTVAGATRYQDSYMERTVIPTAGTEYIA
                     IFYARASENDYPVRCHFYNPNTVVSSENSSGYKSRSSDGLSIIRLSTDWQLCWVKWTQ
                     TATDQAKTVIIGRHGPQVGGKEGVWVEICAPAIFEGNLAGDWSPAYEDQDERVSVVES
                     NFKQRADSLEAGVSRLTEGLRTKADISSLNVTAENIRQSVKSLETDTQNKLNQKLSQA
                     EFEVRAGSIRQEILNATKDKANKSELTQTAEELASKIASVQVGGRNYIRGTKRMMLAR
                     GLWASGTFRPSGAGTAKTIDVSDSPATGFDKAIRLTSSNARDQIGIAQDGFYISQGTY
                     TMSCWVKGRRGQKVKLQTYWQVNDNSGISPIFTLKDENWTKLSFTSARNRAGVASIGY
                     VYLVNAEVGEYLDVLAPQLEDGSLATSSKEAPEDIEGQISTVESTFKQRADSLAAGVN
                     RLTEGLRTKADISALNVTAENIRQSVKSLETDTQNKLNQKLSQAEFEVRAGSIRQEIL
                     NATKDKASKSELTQTAEELASR"
BASE COUNT          473 a          541 c          442 g          658 t
ORIGIN      
        1 atccgactag ccaactcctc agctgtctgc gtgagttctg acttgctggc tttatccttg
       61 gttgcgttca ggatttcctg acggatagag ccagctcgca cctcaaattc agcctgactc
      121 aacttctgat ttagtttgtt ctgcgtgtct gtctcaaggc tcttcacaga ttgccggata
      181 ttttcagcag tcacattgag tgcgctgata tcggctttag ttctaaggcc ttcagtcaga
      241 cgattcacac cagcggcgag tgaatcagca cgctgtttga aggtcgattc aactgttgaa
      301 atctgacctt ctatatcttc aggagcttct tttgagcttg ttgccaaact tccgtcttcc
      361 agctggggcg caagaacatc taaatattct ccgacctcag catttacgag atacacatag
      421 ccaattgatg cgactccagc cctatttcta gcgctagtaa acgatagctt tgtccaattt
      481 tcatccttta atgtaaagat gggcgaaata cccgaattat cattgacttg ccaataagtt
      541 tgtagcttga ccttttgacc tcttctgcct ttgacccaac aagacatcgt gtatgtgcct
      601 tgcgagatat aaaatccatc ttgagcaata cctatttggt ctctagcatt gcttgaggtc
      661 aatcgtatcg ctttatcaaa gccagttgct ggactgtctg atacatcaat cgtctttgcc
      721 gtcccagcgc ctgacggtct aaaagtacct gatgcccaca atcctctggc tagcatcatg
      781 cgctttgtac cccggatata attcctaccc ccgacctgca cactcgctat cttactagct
      841 agctcctcag ctgtctgcgt gagttctgac ttgttggctt tatccttggt tgcgttcagg
      901 atttcctgac ggatagagcc agctcgcacc tcaaattcag cctgactcaa cttctgattt
      961 agcttgttct gcgtgtctgt ctcaaggctc ttcacagatt gccggatatt ttcagcagtc
     1021 acgttgagtg agctgatatc cgctttggtt ctaagccctt cagtcagacg gcttacacca
     1081 gcttcgagtg aatcagcacg ctgcttaaag ttggattcga ctactgagac acgttcgtct
     1141 tggtcttcgt atgctggtga ccagtcacct gcaagatttc cctcaaaaat ggcaggggcg
     1201 cagatttcaa cccatacccc ctctttaccg cctacttgag ggccatggcg gccaatgatg
     1261 accgtcttgg cttgatctgt tgcggtttgg gtccatttaa cccagcacaa ctgccagtct
     1321 gtcgagagac ggataataga caagccatct gacgaccttg acttatatcc gctgctgttt
     1381 tctgatgata caaccgtgtt aggattgtaa aaatgacagc gcacaggata gtcattttca
     1441 ctggctcgtg cataaaagat agctatatac tctgtcccag ccgttggtat aacggttctt
     1501 tccatatagc tatcctgata tctagttgct cctgcgactg ttctgataac tttaaaacca
     1561 ttatattcac taaccggctt gtatctcgca agctcttttg tgcctttcag cagatttctg
     1621 cgccctaagt gcacactcgc aatccgacta gctagctcct cagctgtctg cgtgagttct
     1681 gacttgctgg ctttatcctt ggttgcgttc aggatttcct gacggataga gccagctcgc
     1741 acctcaaatt cagcctgact caacttctga tttagtttgt tctgcgtgtc tgtctcaagg
     1801 ctcttcacag attgccggat attctcagca gtcacattga gtgagctgat atcggctttg
     1861 gttctaaggc cttcagtcag acgattcaca ccagcttcga gcgagttggc tcgttgtttg
     1921 aaggtcgatt ctactgttga aatctgacct tctatatctt caggagcttc tgaataagaa
     1981 gtatctacat cgcttatttc aaacttcggc atccaaatcc aaacggttcc ttcttggttg
     2041 aaattgaaca accattcatt tgtggtctgc ttggattcgt ttgtccaacc ttttggaata
     2101 tggacaacat atcg
//
LOCUS       DBERDK010000132         2084 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.132, whole genome shotgun sequence.
ACCESSION   DBERDK010000132 DBERDK010000000
VERSION     DBERDK010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.132"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..1014
                     /locus_tag="ACL73L_002056"
     CDS             <1..1014
                     /locus_tag="ACL73L_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGS0772951.1"
                     /translation="DSKVFIDATGNATIASASGADVYEKNSSYGCLMRVGGVDISKII
                     EHIEKTKQWNYIEGYNQWLEEVLANRKYRATGAVLKDPVSYDHAPMLSKDDHFMNDEK
                     WNYIKERWEKFRFIYTLEVSLFRNELKKAVESGDFNFEIYHDDKSGVTMNGDGISYGS
                     WGQNVALINVAKAFGFNPKYMEDESHANILSRKYNLTFLSFLKKYVPGFENSFILDQG
                     TRATNRSCRHIDNSSMMDEILDFPMYTFKTMYSYQTPKEITYKSIVGGKLNNLFVIGK
                     GAYQSESFRSQISCMLMGISSAAAAKTIIEDKSNTLNINRDLFKSNLDILFTWEFSNW
                     ITD"
     gene            1036..1491
                     /locus_tag="ACL73L_002057"
     CDS             1036..1491
                     /locus_tag="ACL73L_002057"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HGS0772952.1"
                     /translation="MNDTNKNQYFVYEFDTSFVEMSYSQFFEGYCILFSKEPYPSLSD
                     MPMEFRKEFLMEMSILGDAMLEVLNAYRINYDILGNSTPVLHAHLFPRYMWEEEERRK
                     TVVHRYDSSVFSNPEFEFTIEKHGPIIKKLRSAIDRHYREYQQLLDDSN"
BASE COUNT          752 a          242 c          372 g          718 t
ORIGIN      
        1 gattctaaag tttttattga tgctacaggg aatgcaacaa ttgcttctgc tagtggagct
       61 gatgtttatg aaaaaaattc ttcttacggt tgtttaatga gagttggagg agttgatatc
      121 tcaaagatta tagaacatat tgaaaaaaca aagcagtgga actatataga aggatataat
      181 cagtggttag aagaagtatt agcaaatcga aagtacagag ccactggagc agttttaaaa
      241 gatccagttt cttatgatca tgcccctatg ctttcaaaag acgaccattt tatgaatgat
      301 gaaaaatgga attacattaa agaaagatgg gaaaaattta gatttattta cactttagaa
      361 gtatcactat ttaggaatga attaaaaaaa gctgtagaat ctggggattt taattttgaa
      421 atttatcatg atgataaatc tggagtcact atgaatggcg acggtataag ctatggttca
      481 tggggtcaaa atgtagcatt aattaatgtt gcaaaagcat ttggattcaa tcccaaatat
      541 atggaagatg aatcacatgc taatattctt tctagaaaat ataatttaac atttttatct
      601 tttcttaaaa aatatgtacc aggttttgaa aattctttta tccttgatca gggaactaga
      661 gctactaaca gatcatgtag gcacattgat aattcatcta tgatggacga aattcttgat
      721 tttccaatgt atacatttaa gactatgtat agctatcaaa ctcctaaaga aataacgtat
      781 aaatcaattg taggtggaaa attgaataat ttgtttgtaa taggtaaagg tgcttaccaa
      841 tccgaaagtt ttagatcaca aattagttgt atgttaatgg ggatatcatc agcagcagcg
      901 gcaaaaacta ttattgaaga taaaagcaat actctaaaca ttaatagaga tttgtttaaa
      961 tcgaacttag atatattatt tacatgggag ttttctaatt ggattactga ttaacaaata
     1021 ataagggaat attaaatgaa tgatactaat aaaaatcaat actttgttta tgaatttgat
     1081 actagctttg tagaaatgtc ttattctcag ttttttgaag gatattgtat tcttttttca
     1141 aaagaaccct atccttcttt atctgatatg ccaatggaat ttagaaaaga atttttaatg
     1201 gaaatgagta tattaggtga tgcgatgctt gaagtgttaa atgcctatag aattaattat
     1261 gatatcttag gaaattcaac acctgtactt catgctcatt tatttccgag atacatgtgg
     1321 gaagaggaag agagaagaaa aactgtagtt catagatatg attcttcagt ttttagcaat
     1381 cctgagtttg aatttactat agaaaagcat ggaccaatta ttaaaaaact gaggtctgct
     1441 attgatagac attatagaga gtatcaacaa ttattagatg attcaaactg aactccacat
     1501 tttcgattag atttaatacc ttgtatatta caactaggtt gtggatgata taatttttcg
     1561 aaaaatatat tttaggaatg acttataaag taattatatc agaggttatt aaatcaatgg
     1621 tccaagctta tgctccttat tcttatttta aggttggagc tgcgttagaa actgaagata
     1681 aaaatttttt tctggatgta atatagaaaa tgttgctttt ctcctacttg ctgtgcagaa
     1741 cgagtatatt tttacaaagc tgtatttaaa ggagaacgtt cgttttcaaa aatatgtatt
     1801 gtaggcggaa aaaaaggtat tttatcatct tttatagcat cttgcagggt gtcttgtcag
     1861 gtaatgatag aattttgtaa tcttgattca tttgtagtta tattggctag tgatgttgat
     1921 acttataaag tatataaact taaagaacta ctaccagtta gttttaatca agaaaattta
     1981 taacaggagg gaactatgga attagttgaa tttaaaaatg gacatgttaa tacggttcat
     2041 gagcatatcg caataacgaa agtaaaagag tttctaaata atca
//
LOCUS       DBERDK010000133         1908 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.133, whole genome shotgun sequence.
ACCESSION   DBERDK010000133 DBERDK010000000
VERSION     DBERDK010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.133"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..1107)
                     /locus_tag="ACL73L_002058"
     CDS             complement(<1..1107)
                     /locus_tag="ACL73L_002058"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772953.1"
                     /translation="MSKLILNLKLNSFLFVIILLLVVVTPTHTLIFDALIPTKIDNLW
                     REFLLVISFLCILKINAGKIKIGKLGGPIIVMGGIGLVYTICSDRPFTALNLFRIYMF
                     PAVIYFIIINCNFRKERLLILKQAHVYTACILALWGIFQAWVLKDQFLIKIGYPSQGN
                     FLKSTAFYIGGFFGQQRVTSTFSAPNLAGVYFGISLIILLSIFDTIKSNRLVLFSIVA
                     AFVLTFSRSAIISTLVGIVFFQRKKLFSTTKINVMTLVIFPLIFLIVLVIFYLYPENV
                     IINMLYSSYSSTLNLTDSSAVKHLEDLWLPLLKVIDYPLGLGFGNNGPIVLSLYHSAN
                     LVESSIYLLAYDFGILGMFIYLFPYFYTIFVYKKY"
     gene            1334..1696
                     /locus_tag="ACL73L_002059"
                     /pseudo
     CDS             1334..1696
                     /locus_tag="ACL73L_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001857010.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          720 a          327 c          275 g          586 t
ORIGIN      
        1 atatttttta tatacaaaaa tcgtataaaa ataagggaac aaatatatga acattcctaa
       61 aataccaaaa tcatatgcaa gaagataaat agaagattct actaaatttg ctgagtgata
      121 gagtgacaga acaattgggc cattattacc aaatccaagt cccaaaggat aatcaataac
      181 ttttagaaga ggaagccata agtcttccaa atgcttaacc gctgaagaat ctgtcaaatt
      241 aagtgttgaa gaataactgc tatagagcat atttataata acattctcag gataaagata
      301 aaatataact aaaacaatca aaaaaattaa ggggaagatt accaaggtca taacattaat
      361 cttcgtcgtt gaaaacaatt tcttacgttg aaagaatacg attcctacaa gagtacttat
      421 aatagcacta cggctaaatg taagaacaaa agctgctaca attgaaaaca ataccaatct
      481 attactcttt atagtatcaa atatagagag taaaatgatc aaagatattc caaaataaac
      541 tccagctaaa ttaggagcag aaaaagtgct agtaactcgt tgttgaccga aaaaaccacc
      601 aatataaaaa gctgtactct ttagaaaatt tccttgggaa ggatatccta ttttaatcaa
      661 aaactgatct tttaacaccc acgcctgaaa aattccccat aaagctaata tgcaagcagt
      721 atatacatgc gcttgcttca atatcagtaa tctctccttg cgaaaattac aatttattat
      781 tataaaatat attacagcag gaaacatata aattctaaaa agatttaaag ctgtaaatgg
      841 cctatctgaa cagatagtat aaactaaacc aattcctccc ataacaatta ttgggccccc
      901 tagctttcca attttaattt ttcctgcatt tatctttaga atacatagaa aagaaattac
      961 tagaagaaac tctctccata aattatcaat tttcgttgga attagtgcat caaaaattaa
     1021 ggtatgagtc ggagtaacaa caactaaaag taaaattata acaaataaaa aagaatttaa
     1081 tttcaagttc aatattaatt tactcaccta tttctcctta tattatattt agtgtagttc
     1141 gctactgcta aagtccaagt ggtacacttg gaataagcca ctgtggatta gttcatttcc
     1201 tccaatcgct tgacgatttc tgaaaggctc taaccacagc tttttgtttc aattggtaat
     1261 gagtttgata gcgcataaac gttttatatt gtgtaaggta acaaggcaca aagttcgtgg
     1321 ataaccaagt gagcttggga atcgtttagg tgacgttatc ttagttgaga ttcgaagtat
     1381 tcatatcttc gataagcctc atcaattaca agcttttgct ggaccgaatc cttctattta
     1441 ccagtcaggt caaatcgatt tagcagggaa aatggtcaaa ctgggttccc ctcatctacg
     1501 ttgggcactc atacaagcta ccaaagcata tgctcgcttt tcgcctgttt ttaaagccta
     1561 tcttaggact aagttgtaac aaagaaagca ttacaatgta gccatcattc accttgccaa
     1621 aaaacttatt agaaatctgt tttatctcct aaaaaacaag ctagcatttg acgaaaaaaa
     1681 gtgttctaac tcctgaattt gccacaagat aacctttgaa aaaataaact ttttcaagga
     1741 cattttgccg tgctctaaaa tcatccactt tgaaacaaga agtatttcag attaaactat
     1801 tgaattttct tatagttagt ctttgtatat aataaattca tcatatctaa gattatatat
     1861 tctaaaatgg atagcatcat gacagatttt aaacatagtt ttttgaca
//
LOCUS       DBERDK010000134         1806 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.134, whole genome shotgun sequence.
ACCESSION   DBERDK010000134 DBERDK010000000
VERSION     DBERDK010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.134"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            461..1806
                     /gene="mobQ"
                     /locus_tag="ACL73L_002060"
                     /pseudo
     CDS             461..1806
                     /gene="mobQ"
                     /locus_tag="ACL73L_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019213953.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
BASE COUNT          870 a          223 c          294 g          419 t
ORIGIN      
        1 gaagaaagta ttgatttaac aaaagatgaa ttttataagt taataaaaac gttaaatgat
       61 gaagaaataa gattaaacat aatggaaatt ttagaagaaa gaatagatga taatgtagaa
      121 aaatcatcta aagatgaaat aacataattg tcccttggtt acaagggcgc aattatacac
      181 cctaaagggt acttgcgtcc tgcggagtca aacccttgca gggagcaaca accattcgct
      241 ttttaaaagt caagggtaaa taaactcgct aaggctcgcc cttgactttt aaaatttgaa
      301 atgaaagtaa caattaagcc gacatactga aacaacaaaa attaattcag tagtcggctt
      361 ttttaattct atcatttcaa aacgctcatt cacaaagagg atataaaaaa actattaagc
      421 ctccacctaa aacaacaaag aaagaaagga agtgataaaa atggcagaca gttttcattt
      481 ttcagtaaac ataatatcaa gaggaaaagg caaaagtgca gtagcaagtg cagcatatat
      541 aagtggagaa aaaataaaaa atgaatggga cggagtaacc catgactaca caagaaaaga
      601 aaaaatatta gtaaaaaata taatattgcc tgatcatata ccaaaagaat ttaatgatag
      661 gtctatctta tggaataaag tagaaatggc agaaaaaaat tctaatgcac aactagcaag
      721 acaattcata ataggactac caaaagagtt atctttaagt gaaaataaaa acctggttga
      781 aagatatata aaagaaaatt taacatcaca aggaatgata gtagattatg caattcatga
      841 tgagagtcaa gataaaaatg gaaatatcca ttgtcatata atgaccataa tgcgaccaat
      901 aaacgaaaaa ggagagttct tagccaagtc aaaaaaagaa tatatcctag atgaaaaagg
      961 agaaaaagta ttaaacaaaa atggaaaacc aaagacaaga aaagtagaac taacaacctg
     1021 gaacgataca ggcaatgtag aaaaatggag agaaaatttt tcagatcttt gtaacaaata
     1081 tttagaaaga gcaggagcag aaaaaagagt agaccatagg agttttaaaa gataaaattc
     1141 agattatcta ccgacaatcc atttaggatc agcagcaagt gcaatggaaa gaaagggaat
     1201 agaaactgac aagggaaact ataatagaga aataagaaaa tataatcaac ttgtaaaaac
     1261 aataaaagaa gagataaaaa cattaaaggg ttggatagga aatttattag ataacttatc
     1321 tactgcttat gaaaaattta aggatatcga aagagataag gtaatagaca accctaaact
     1381 ttttaatcta acaaattatc tattaactta ttcagaaatt caaaaagaaa aaagtaaata
     1441 tctaaaagga tatgcaaaaa ctaataaaga aaaatatgac ttcaaaaagc taacaagtgc
     1501 atatagttat ttaagaaaaa acaacataga aaccataggt cagttacaaa caaaaataga
     1561 aactttaaag tctaatagtt ataggctaaa taaaaaagca aagacaatcc ataaagaaat
     1621 ggaagatgta gaaaagaaaa ttctatacta tgaaatctac aaagccaaaa aagaagttta
     1681 tgaagaatat caaaagaaaa atatattcac aaaagaagca ttttataaca aacataagaa
     1741 agatatagac caatataaag tagtaagcgg gaaattaaaa aaactcctat cagataagga
     1801 aaaact
//
LOCUS       DBERDK010000135         1711 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.135, whole genome shotgun sequence.
ACCESSION   DBERDK010000135 DBERDK010000000
VERSION     DBERDK010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.135"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            79..>1711
                     /gene="pspC"
                     /locus_tag="ACL73L_002061"
     CDS             79..>1711
                     /gene="pspC"
                     /locus_tag="ACL73L_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein PspC,
                     LPXTG-anchored form"
                     /protein_id="HGS0772954.1"
                     /translation="MFKSNHERRMRYSIRKFSVGVASVAVASLVMGSVVHATENEGTT
                     QAPTSSNRGNESQAEQRRELDLERDKVKKEVREYKEKKVKELYSKSTKSRHKKTVDIV
                     NKLQNINNEYLNKIIQSTSTYEELQKLMMESQSEVDKAVSEFEKDLSSSSSSGSSTEP
                     EASDTAKPNKPTELEKKVAEAQQKVEEAEKKAKDQKEEDHRNYPTITYKTLELEIAEF
                     DVKVKEAELELLKVKAKESRDEKKIKQAEAEVESKQAEATRLKKIKTDRKKAEEEAKL
                     KEAVEKNAATSEQGKPKRRVKRGALGEQATPDKKDYFEKDFRPAFNKNQQMVAIQESL
                     NKLDDETKTVPDGAKLTGEAGNAYNEVRDYAIKVVSENKKLLSQTAVTMDELAMQLTK
                     LNDAMSKLKEAKAKLVPEVKPQPENPEHQKPTTPAPDTKPIPQPEGKKPSVPDINQEK
                     EKAKLAVATYMSKILDDIQKHHLQKEKHRQIVALIKELDEFKKQALSEIDNVNTKVEI
                     ENTVHKIFADMDAVVTKFKKGLTQDTPKEPDNKKPS"
BASE COUNT          708 a          285 c          358 g          360 t
ORIGIN      
        1 aataatgtag caaatttcat atacaattca tattagcagg atatactgta gttaattaaa
       61 attatggaga atatagacat gtttaaatca aatcatgagc gaagaatgcg ttattccatt
      121 cgtaaattta gtgtaggagt agctagtgta gctgttgcca gtcttgttat gggaagtgtg
      181 gttcatgcga cagagaacga gggaactacc caagcaccca cttcttctaa taggggaaat
      241 gaaagtcagg cagaacaacg tagagaactc gatttagaac gagataaggt aaagaaagag
      301 gtcagggaat ataaagaaaa aaaagtgaaa gagctctatt caaaatcaac taaaagtcga
      361 cataagaaga ctgtagatat agttaacaag ttgcaaaaca ttaataacga gtatttgaat
      421 aaaataattc aatcaacctc aacatacgaa gaactacaga aactgatgat ggagagtcaa
      481 tcagaagtag ataaagctgt gtctgaattt gaaaaggact tatcttcttc gtcaagttca
      541 ggctcttcca cggaaccgga agcttcagat acagcgaagc caaacaagcc gacagaacta
      601 gaaaaaaagg tagcagaagc tcagcagaag gttgaagaag ctgagaaaaa agccaaggat
      661 caaaaagaag aagatcaccg taactaccca accattactt acaaaacgct tgaacttgaa
      721 attgctgagt tcgatgtgaa agttaaagaa gcggagcttg aactattaaa agtgaaagct
      781 aaggaatctc gagacgagaa aaaaattaag caagcagaag cggaagttga gagtaaacaa
      841 gctgaggcta caaggttaaa aaaaatcaag acagatcgta aaaaagcaga agaagaagct
      901 aagttgaagg aagctgttga aaagaatgca gcgacttcag agcaaggtaa accaaagagg
      961 cgggtaaaac gaggagctct tggagagcaa gcaacacctg ataaaaaaga ttattttgaa
     1021 aaagacttcc gtccagcttt caataaaaac cagcagatgg tagccattca agaatccttg
     1081 aacaaactag atgatgaaac aaaaactgtt ccagatgggg ctaaactcac aggagaagct
     1141 ggaaatgcct ataatgaggt cagagattat gcaataaaag ttgtttctga aaacaagaaa
     1201 cttctatcac agacagcagt gacaatggat gaactggcaa tgcaattaac caaattgaac
     1261 gatgccatgt ctaaattgaa agaagctaaa gcgaaattgg taccagaggt taaaccgcag
     1321 ccggaaaatc cagagcatca aaaaccaaca actccagctc cggataccaa accaatccct
     1381 caaccagagg gtaagaaacc aagcgtacca gatattaatc aggaaaaaga aaaagctaag
     1441 cttgctgtag caacctacat gagcaagatt ttagatgata tacaaaaaca tcatctgcag
     1501 aaagaaaaac atcgtcagat tgttgctctt attaaggagc ttgatgagtt taaaaagcaa
     1561 gctctttctg aaattgataa tgtaaatacc aaagtagaaa ttgaaaatac agtccacaag
     1621 atatttgcag acatggatgc agttgtgact aaattcaaaa aaggcttaac tcaggacaca
     1681 ccaaaagaac cagataacaa aaagccatct g
//
LOCUS       DBERDK010000136         1666 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.136, whole genome shotgun sequence.
ACCESSION   DBERDK010000136 DBERDK010000000
VERSION     DBERDK010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.136"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            10..1011
                     /locus_tag="ACL73L_002062"
     CDS             10..1011
                     /locus_tag="ACL73L_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGS0772955.1"
                     /translation="MKNINGKKVTIYDIARLSGFSPKTVSRVINGGVNVKEETYQAIQ
                     KVIEELSYIPNAYAKNLTKKEAINILISVKKIDSFPLIWFHTLLDKVLRTCKEFGVNA
                     IVEYFGEEDTISNSIISSTGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSG
                     VVYVSNNNFQATYDMMKAVTEEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNYFMDL
                     KVIYGLSTIDSVYSYAMQHLTTQNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISII
                     GFDNIPISQYYTPALSTIAPNYVKLAKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF"
     misc_binding    1223..1452
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT          558 a          227 c          334 g          547 t
ORIGIN      
        1 aggactgaaa tgaaaaatat aaatgggaaa aaggtaacta tttatgatat tgcacgttta
       61 tcaggttttt ctccaaaaac agtttcacga gttattaatg gtggagtaaa tgttaaagaa
      121 gaaacttatc aagcgattca aaaagttata gaggaattgt cgtatattcc aaatgcgtat
      181 gcaaaaaatt taactaaaaa agaagctata aatatattga tttctgtaaa aaaaatcgac
      241 tcttttcctt taatttggtt tcatacgctg ttagataaag tgttacggac gtgtaaagaa
      301 tttggtgtta atgctatagt tgagtacttt ggagaggagg atacaattag taattcaatt
      361 atatcaagca caggtagcct agtagatggt gttattgttt tttatgaaag tgtagatgat
      421 atcagaattc agtatttaaa gaagaaccat atgccttttc ttgttttcgg tgaatctcaa
      481 acgtctggag tagtctatgt atctaataat aattttcaag ctacttatga tatgatgaaa
      541 gctgtaactg aagaaaaatt taaaaatatg tggttgctta tgggaggaga atcccatgtt
      601 aataaggatc gtgaaagagg tgtgcgttct tttctgaatg ataaaaatta ctttatggat
      661 ttaaaagtca tttatggctt atctacaatt gattcagttt attcttatgc aatgcagcac
      721 ttgactaccc aaaattatcc ggacattatt tttgtttcag gagatgaaaa ggttcagggc
      781 ttgattcgtg cgtgctatga gaaaggaatt cttattccag atgatatttc tatcattgga
      841 tttgataata ttcctatttc tcagtattat actccagctt tgtctacaat tgctcccaat
      901 tatgttaaat tggctaaaga aatgatagaa ggtgttttag caattattaa aggggaaagt
      961 gtcacatctg ttgaagtttc tcctaaattt gttaggagac agagtttcta gtattgttta
     1021 attcttgaaa atcctagtgg aaaagatgga gtgttctaaa ataagttctg tttagcctcc
     1081 attcagtttc tattgagttg gatccaattt ctaacaagtg ggacatgttc atgttcgatc
     1141 taaggaagac atggtcacat tcgaatgata ttttcaaaaa aatcgtaaaa aagtcttgac
     1201 aaagaaaaaa caaagtatta aaataagttc aacaaatcag aaaagtaact atttttgatc
     1261 ttcagggagc ctgtggtggt tgtgaatagg tggttggaag tagtgaaagt gggctgattt
     1321 tgaaaatgaa tttgaaacaa tgaaaattcg gtgcgcacac cttacagtgc agcttgttgt
     1381 taaacaaggc agagatgtaa agggatagtc cctttataat tgaggtggca ccgcgttacc
     1441 aacgccctca cacggaagta tattctgtgt gtgggctttt ttctatccgt cgtttggttt
     1501 atcttttatt agggccttaa gtcgctttga tgaacttgag ttctatctac agctccttgc
     1561 ctactactaa aagcaaacaa aaaggctgga ctaatacgaa aaaagctagt aatactcaat
     1621 gaaaatcaaa gagcaaacta ggaaactagc cgcaggctgc tcaaaa
//
LOCUS       DBERDK010000137         1648 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.137, whole genome shotgun sequence.
ACCESSION   DBERDK010000137 DBERDK010000000
VERSION     DBERDK010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.137"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            74..506
                     /locus_tag="ACL73L_002063"
                     /pseudo
     CDS             74..506
                     /locus_tag="ACL73L_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811998.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
     gene            695..994
                     /locus_tag="ACL73L_002064"
     CDS             695..994
                     /locus_tag="ACL73L_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HGS0772956.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKASVDEANIKTLYSEKLIGISIPIK"
     gene            1255..1578
                     /gene="tnpB"
                     /locus_tag="ACL73L_002065"
     CDS             1255..1578
                     /gene="tnpB"
                     /locus_tag="ACL73L_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGS0772957.1"
                     /translation="MMIQLSDLGQVHLVCGKTDMIQGIDSLAYLVKTHFELDPFSGQV
                     FLFCGGRKDRFKALYWDGQGFWILYKRFENGKLTWPSTEKDVKALAPEQVDWLMKGFS
                     ITPQI"
BASE COUNT          563 a          327 c          286 g          472 t
ORIGIN      
        1 aagattatac cacattgtgt actatttttg gttcatttca ctataataat aacgatacta
       61 gataatgagg aaaatgcaat ggcaaaccca actttcggag aaaaaaagcg aatacagact
      121 atattgctcg ctatggcgta tatgcagtta tccctaatcc tgaacaaaaa caaattgttc
      181 ttgttcaaga accaaatggt gcttggttcc taccatgtgg agaaattgaa gcaggtgaaa
      241 atcatcagga agccctaaag cgtgagttga ttgaagagct tggtttcaca gcagaaattg
      301 gtacctatta cggacaagct gacgaatatt tctattctcg tcatcgtgat acctactact
      361 acaatcctgc ctacctctat gaagcaactc ctttcaaaga agtacaaaag ccactagaaa
      421 actttaatca tattgcctgg ttccctattg acgaggctat caaaaacctt aaacgtggta
      481 gccataaatg ggccattgaa tcttgaaaaa aacagcataa gattgactaa tactcttcga
      541 aaataaaatt caaaccacat cagcgtcgcc ttaccatact caagtatagc ctgcggctag
      601 cttcctagtt tgctctttta ttttcattga gtataaatca agcgacttta ttcaaaaagt
      661 gctataatag aattagaaaa tcggaggaaa tattatgaag cttatcaata cgacaaactc
      721 acactcgcaa cttgtaaaaa gtcagctaga aagtaccgat gcaacccttg ttgaggttta
      781 ttctgcaggg aatacagatg ttatttttac ccaagctccg cttcactatg aaatcctcat
      841 ctcaaacaaa caccgtgcta ttcgtgagcc tgaaatcgaa accattcaag aatttttctt
      901 gaaacgtaaa attgataagg cctctgttga tgaagccaat atcaagacac tctactcaga
      961 gaaattaatt ggaatttcaa tcccaatcaa gtaagaaaat tgagtaaaaa ctcgatttga
     1021 gagaaagtcc aataagtctt tccataataa aacgcatagt agcaagagtt tctacaccca
     1081 ctattatacg tttttatcat tttgaaatct gctgctaatc aatgaagaag caaagatcaa
     1141 actaggcagc tagttacaag ttacgtaagc gcgtcataac aaggtatcta tcctaattcc
     1201 ccaacttcca gtatactaga taaaaaataa ttatcggagg aaaagtattg tactatgatg
     1261 attcaactaa gtgatttagg tcaagttcac cttgtttgtg gcaagacaga tatgatacaa
     1321 ggaatcgatt cactggctta tctcgttaaa acccactttg aattggatcc tttctccggt
     1381 caagtctttc tcttttgtgg tggacgtaaa gaccgcttta aagcccttta ctgggatggt
     1441 caaggatttt ggatactata taaacgcttt gagaacggaa aactgacttg gcccagtaca
     1501 gaaaaggatg tcaaagctct cgcacctgaa caagtagact ggcttatgaa gggcttttct
     1561 atcactcccc aaatatagtg gatttaaact agaatagtac acccctactt ctaaaacatt
     1621 gttagaaatc gatttgactg tcctgatc
//
LOCUS       DBERDK010000138         1614 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.138, whole genome shotgun sequence.
ACCESSION   DBERDK010000138 DBERDK010000000
VERSION     DBERDK010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.138"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..1238
                     /locus_tag="ACL73L_002066"
     CDS             <1..1238
                     /locus_tag="ACL73L_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HGS0772958.1"
                     /translation="LDIKDPNIQILDIINKDTHKEIIAKLDYDAPSCPECGSQMKKYD
                     FQKPSKIPYLETTGMPTRILLRKRRFKCYQCSKIAVAETSLVKKNHQIPRIINQKIAQ
                     KLIEKISMTDIAHQLSISTSTVIRKLNDFHFKHDFSRLPEIMSWDEYAFTKGKMSFIA
                     QDFNNLNIITVLEGRTQAIIRNHFLRYDRVVRCRVKIITMDMFSPYYDLARQLFPCAK
                     IVLDRFHIIQHLSRAMSRVRVQIMNQLDRKSHEYKAIKRYWKLIQQDSRKLSDKRFYR
                     PTFRIHLTNKEILNKLLSYSEDLKHHYQLYQLYQLLLFHFQNKEPEKFFGLIEDNLKQ
                     VHPIFQTVFKTFLKDKEKIVNALQLHYSNAKLEATNNLIKLIKRNAFGFRNFENFKKR
                     IFIALNIKKERTKFVLSRA"
     gene            complement(1441..>1614)
                     /locus_tag="ACL73L_002067"
     CDS             complement(1441..>1614)
                     /locus_tag="ACL73L_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein A"
                     /protein_id="HGS0772959.1"
                     /translation="WVKDGDTWYYLEASGAMKASQWFKASDKWYYVNGSGALAVNTTV
                     DGYGVNANGEWVN"
BASE COUNT          508 a          347 c          267 g          492 t
ORIGIN      
        1 tactagacat taaagaccct aatatccaga ttttagacat catcaataaa gatacacaca
       61 aggaaatcat cgccaaactg gactacgacg ccccatcttg ccctgagtgc ggaagtcaaa
      121 tgaagaaata tgactttcaa aaaccttcta aaattcctta tcttgaaacg actggtatgc
      181 ctactagaat tctccttaga aagcgtcgat tcaagtgcta tcagtgctca aaaatagcgg
      241 tcgctgagac ttccctcgtc aagaaaaatc accaaatccc tcgtatcatc aaccaaaaga
      301 ttgctcaaaa gttgattgaa aagatttcta tgactgatat tgcccatcag ctttccatct
      361 caacttcaac tgttattcgt aagctcaatg attttcactt taaacatgat ttttcgcgtc
      421 ttcctgagat tatgtcttgg gatgagtatg cttttacaaa gggaaagatg agcttcattg
      481 cgcaagattt taacaatctc aatatcatca ctgttcttga gggaagaaca caagctatca
      541 ttcgaaatca ctttcttcgc tacgatagag ttgtccgatg tcgcgtcaaa attattacta
      601 tggatatgtt tagcccttac tatgacttag ctagacagct tttcccgtgt gctaaaatcg
      661 ttctagatcg tttccatatt atccaacatc tcagccgtgc catgagtcgt gtgcgtgttc
      721 aaatcatgaa tcaattggat cgaaagtctc atgaatacaa ggctatcaag cgctactgga
      781 aactcattca acaggatagc cgtaaactga gtgataagcg attttatcgt cctacttttc
      841 gtatacattt aaccaataaa gagattttaa acaagctttt gagttattcg gaagacttga
      901 aacaccacta tcagctctat cagctctatc agctcttgct ttttcacttc cagaataagg
      961 aaccagagaa atttttcggg cttattgagg acaatcttaa gcaggttcat cctatttttc
     1021 agactgtctt taaaaccttc ctcaaagata aagaaaagat tgtcaacgcc cttcaactac
     1081 actattctaa tgccaaactg gaagcgacca ataatctcat caaacttatc aagcgcaatg
     1141 cctttggttt tcgaaacttt gaaaacttca aaaaacggat ttttatcgct ttgaatatca
     1201 aaaaagaaag gacaaaattt gtcctttctc gagcttagct gacttcaacc cactacagtt
     1261 gacaaagagc caaaaaaact ccgtagtctc tagcgctata aatccggcta gagattacag
     1321 agttattttt taatctaaac ttcctacaag ggtatattat ctactgaaag gatatggtat
     1381 ttgtagggaa tactttaaag agttcttaca ggaagtcagt attaactagt tatattaggt
     1441 ttagtttacc cattcaccat tggcattgac tccatagcca tctacagttg tgttgactgc
     1501 aagggcacct gagccattga catagtacca tttatctgat gctttgaacc attggcttgc
     1561 tttcatagca cctgatgctt caagatagta ccaggtatct ccatctttca ccca
//
LOCUS       DBERDK010000139         1435 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.139, whole genome shotgun sequence.
ACCESSION   DBERDK010000139 DBERDK010000000
VERSION     DBERDK010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.139"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            77..1255
                     /locus_tag="ACL73L_002068"
     CDS             77..1255
                     /locus_tag="ACL73L_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HGS0772960.1"
                     /translation="MTLSKKQLQLRAKILETVYTLGPISRIEIATKTGITPATTSSIT
                     NELIKENILLELGEDEHDTSVGRKKILLDIQAKRFYYIGCELSEKHFTFALGDNLGNI
                     LKEEKEIVTKQLIQEKGNQLINQTLKQFLNNCSDYEIEAIGIALPGRYLDDYKITTNN
                     PLWQHIDLEMIQSHFDKPLFFSNNVNCMAIGKRLFSRQQNDPNFAYFHFARGMHCSYI
                     YDGNIYGKGNLMIGEIGHTVVSSEGEECSCGRKGCLQTFAGESWLIKKSKILYHQSPY
                     SLLPSLVKNADDIDIQVILTAYQLGDTGIITLIHQALLYLSQTILNISMMIDSQKIYL
                     HSPLLTNQHIIQKLYSEMNYKPKLLYNRLPEVIIEPYNDFTAAHSAIALCLYHTILHS
                     "
BASE COUNT          527 a          263 c          211 g          434 t
ORIGIN      
        1 aagattaaca tctacattta tccatagcaa aaaatcatag gattttaaga atctgacaac
       61 taggaagaaa actaatatga ccttatctaa gaaacaatta caactacgag ctaaaatact
      121 ggaaacagtt tacacattag gtcccatttc acgaattgaa atcgctacta aaacaggaat
      181 aacacctgca acaactagca gtatcacaaa tgagctgatt aaagaaaaca ttctcttaga
      241 acttggtgaa gacgaacacg atacaagcgt agggagaaaa aaaatcctgc tagacattca
      301 agctaaacga ttttactaca ttggttgtga actatctgaa aaacatttca cttttgcttt
      361 aggagataat ctaggaaata tcttaaagga agagaaagaa attgttacta aacagttaat
      421 ccaagaaaag ggaaatcaac tcattaacca gacactaaag caattcttaa ataactgctc
      481 tgattatgaa atagaagcaa ttggaatcgc gctacctggt cgctatttag atgattataa
      541 aataacaaca aacaatcctt tatggcaaca tatcgactta gaaatgattc aatcccactt
      601 tgataagcct ttgtttttct ctaacaatgt taactgtatg gctataggta aacgcctatt
      661 tagtcgccaa cagaatgacc ctaattttgc ttattttcat tttgctagag gtatgcactg
      721 ttcttatatc tacgacggaa acatttatgg gaaaggaaat ctaatgattg gagaaatagg
      781 gcatacagtt gtttcatctg agggagaaga atgtagctgc gggcgcaagg gatgcttaca
      841 aacttttgct ggagaatctt ggttaatcaa aaaatcaaaa attctatatc atcaatctcc
      901 ctattctcta cttcctagtt tagtcaaaaa tgccgatgat attgatatcc aagttatttt
      961 aaccgcttat caattaggtg acactggaat aatcacactt atccatcaag ccttactcta
     1021 tcttagtcaa accattttaa atatcagtat gatgattgac tctcaaaaaa tctacctaca
     1081 tagtccttta ctaacaaatc aacacatcat ccaaaaacta tatagtgaaa tgaactacaa
     1141 acccaaactg ctctataatc gcctacctga agtcattatt gaaccttaca atgattttac
     1201 agccgcacac tcagctattg cactttgttt gtatcataca attctacatt cttaattttc
     1261 tattacattt aataaatagg ctaaaaagag ggagtatatc ataaaaagcg aataaacctg
     1321 ttttctgaaa atcagaattc tgttttgttc gctttcttta tatttggaat tatactccta
     1381 tctccccttt ttcataatgt atagtgaaat gaaataataa caggacgaat cgatc
//
LOCUS       DBERDK010000140         1372 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.140, whole genome shotgun sequence.
ACCESSION   DBERDK010000140 DBERDK010000000
VERSION     DBERDK010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.140"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(171..311)
                     /locus_tag="ACL73L_002069"
     CDS             complement(171..311)
                     /locus_tag="ACL73L_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772961.1"
                     /translation="MKFFWGLLAIIFIKPIIGIVKFFWMIISFAVQLLFYKILDWFFK
                     LI"
     gene            complement(308..490)
                     /locus_tag="ACL73L_002070"
     CDS             complement(308..490)
                     /locus_tag="ACL73L_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772962.1"
                     /translation="MGLIKTLAKIYGNYFLTVQGVKVMKTIKKDDNAVVGLGKLFIAD
                     KLMDTARWLIKPEERE"
     gene            complement(492..1271)
                     /locus_tag="ACL73L_002071"
     CDS             complement(492..1271)
                     /locus_tag="ACL73L_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772963.1"
                     /translation="MAEQDLAMQVLQQVVKLPVVKVDRSKFLVDKFSKELGPQDIPTL
                     LEQGPTSLLSQEILDRVANACIRDNVLLASGTSVLAGLPGGLAMAITIPADVAQFYGF
                     SLKLAQELGYIYGYEDLWASRDELSEDAQNTLLLYLGVMLGVNGTAALLRAGGITIAK
                     QVMKTVPNKALTKMLWYPILKKVLRIFGVNLTKGGLAKGMGKFIPILGGIISGGLTFA
                     TMKPMGESLQKELSKLVNYSEVQYQEDVETIRKEAEIIEGE"
BASE COUNT          449 a          299 c          206 g          418 t
ORIGIN      
        1 atagaactga cgaagtcagt aacatatata ctgcaaggtg aagctgacgt ggtttgaaga
       61 gattttcgaa gagtataaaa tgattttact gcttatatta taccatatgg agagagatta
      121 aaaaactagc agtggagttc ctgctagttt tctgcgactt ggattaccat ctagataagt
      181 ttaaagaacc aatccaatat cttgtaaaac agcaattgaa ctgcaaaaga aatgatcatc
      241 caaaagaatt tcacaatccc aataatcggt ttgataaaaa taatggcaag aagtccccag
      301 aaaaatttca ttctctctcc tctggcttaa tgagccaccg agccgtatcc attaacttgt
      361 cggcaataaa aagtttcccc agaccgacaa cggcattatc atctttcttt atcgttttca
      421 ttacttttac accttgcacg gtcaaaaagt aattaccata aattttagct agagttttta
      481 tgagtcccat attactctcc ttcgatgatt tcagcctctt ttcggattgt ttcaacatct
      541 tcttgatatt gaacttcact atagttaact agcttggaca attctttctg caagctttca
      601 cccatcggtt tcatagttgc aaaggttaaa ccacctgaaa tgataccacc caagatagga
      661 ataaatttcc ccattccttt ggccagccct cccttggtaa gattcacacc aaatattctt
      721 aagacttttt tcaaaatagg ataccaaagc atctttgtta aagctttatt aggtactgtt
      781 ttcattacct gtttggcaat tgttatacca ccagcacgta gcaaagcagc agttccattc
      841 acccccaaca tgacgcctag ataaagcaaa agggtatttt gagcatcttc actcaactca
      901 tcgcgtgaag cccaaagatc ctcataacca taaatataac ctaattcttg ggccaatttc
      961 agagagaaac cataaaattg agccacatca gctggaatgg taattgccat agcaagccct
     1021 ccaggtaatc ctgccaaaac agaagtccca ctcgctaata aaacattatc cctaatacag
     1081 gcattagcca ctcgatctaa gatttcttga gataagagag acgttggacc ttgttccaat
     1141 aaggtaggaa tgtcctgtgg acccaattct ttggaaaact tatccactaa aaatttcgaa
     1201 cgatcaacct taacaactgg tagttttacc acttgttgta atacttgcat agctaagtct
     1261 tgttcagcca tatacaaact ccttatttct tttttacacc ttatcatatt atatttccta
     1321 caaaaaaagc attttctaat tagataacat taaaaaatac agaacccctt tt
//
LOCUS       DBERDK010000141         1372 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.141, whole genome shotgun sequence.
ACCESSION   DBERDK010000141 DBERDK010000000
VERSION     DBERDK010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.141"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..>1372
                     /gene="pcpA"
                     /locus_tag="ACL73L_002072"
     CDS             <1..>1372
                     /gene="pcpA"
                     /locus_tag="ACL73L_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein PcpA"
                     /protein_id="HGS0772964.1"
                     /translation="ILSLTTAAVILAAYVPNEPILADTPSSEVIKETKVGSIIQQNNI
                     KYKVLTVEGNIGTVQVGNGVTPVEFEAGQDGKPFTIPTKITVGDKVFTVTEVASQAFS
                     YYPDETGRIVYYPSSITIPSSIKKIQKKGFHGSKAKTIIFDKGSQLEKIEDRAFDFSE
                     LEEIELPASLEYIGTSAFSFSQKLKKLTFSSSSKLELISHEAFANLSNLEKLTLPKSV
                     KTLGSNLFRLTTSLKHVDVEEGNESFASVDGVLFSKDKTQLIYYPSQKNDESYKTPKE
                     TKELASYSFNKNSYLKKLELNEGLEKIGTFAFADAIKLEEISLPNSLETIERLAFYGN
                     LELKELILPDNVKNFGKHVMNGLPKLKSLTIGNNINSLPSFFLSGVLDSLKEIHIKNK
                     STEFSVKKDTFAIPETVKFYVTSEHIKDVLKSNLSTSNDIIVEKVDNIKQETDVAKPK
                     KNSNQGV"
BASE COUNT          533 a          194 c          221 g          424 t
ORIGIN      
        1 atattatcat taactacagc tgcggttatt ttagcagcat atgtccctaa tgaaccaatc
       61 ctagcagata ctcctagttc ggaagtaatc aaagagacta aagttggaag tattattcaa
      121 caaaataata tcaaatataa ggttctaact gtagaaggta acataggaac tgttcaagtg
      181 ggtaatggag ttactcctgt agagtttgaa gctggtcaag atggaaaacc attcacgatt
      241 cctacaaaaa tcacagtagg tgataaagta tttaccgtta ctgaagtagc tagtcaagct
      301 tttagttatt atccagatga aacaggtaga attgtctact atcctagctc tattactatc
      361 ccatcaagca taaaaaaaat acaaaaaaaa ggcttccatg gaagtaaagc taaaactatt
      421 atttttgaca aaggcagtca gctggagaaa attgaagata gagcttttga tttttctgaa
      481 ttagaagaga ttgaattgcc tgcatctcta gaatatattg gaacaagtgc attttctttt
      541 agtcaaaaat tgaaaaagct aaccttttcc tcaagttcaa aattagaatt aatatcacat
      601 gaggcttttg ctaatttatc aaatttagag aaactaacat taccaaaatc ggttaaaaca
      661 ttaggaagta atctatttag actcactact agcttaaaac atgttgatgt tgaagaagga
      721 aatgaatcgt ttgcctcagt tgatggtgtt ttgttttcaa aagataaaac ccaattaatt
      781 tattatccaa gtcaaaaaaa tgacgaaagt tataaaacgc ctaaggagac aaaagaactt
      841 gcatcatatt cgtttaataa aaattcttac ttgaaaaaac tcgaattgaa tgaaggttta
      901 gaaaaaatcg gtacttttgc atttgcagat gcgattaaac ttgaagaaat tagcttacca
      961 aatagtttag aaactattga acgtttagcc ttttacggta atttagaatt aaaagaactt
     1021 atattaccag ataatgttaa aaattttggt aaacacgtta tgaacggttt accaaaatta
     1081 aaaagtttaa caattggtaa taatatcaac tcattgccgt ccttcttcct aagtggcgtc
     1141 ttagattcat taaaggaaat tcatattaag aataaaagta cagagttttc tgtgaaaaaa
     1201 gatacatttg caattcctga aactgttaag ttctatgtaa catcagaaca tataaaagat
     1261 gttcttaaat caaatttatc tactagtaat gatatcattg ttgaaaaagt agataatata
     1321 aaacaagaaa ctgatgtagc taaacctaaa aagaattcta atcagggagt ag
//
LOCUS       DBERDK010000142         1349 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.142, whole genome shotgun sequence.
ACCESSION   DBERDK010000142 DBERDK010000000
VERSION     DBERDK010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.142"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..607
                     /locus_tag="ACL73L_002073"
     CDS             <1..607
                     /locus_tag="ACL73L_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS0772965.1"
                     /translation="SGSGKSTLLKCISSLLEPTSGEVILNGINPYKIRNAKLSSIRRN
                     EVSFIFQAYNLIPSLPVIENIALPLRLSQKKLTIKNVENLLKRMKFNAGLNDFVGTLS
                     GGEQQKVAIARAVIADSDIIFADEPTGALDSVSREVIFELLRELVGAGKCVIMVTHDI
                     ELASKTDRALILKDGKIFKELHRPSGEELYKILEVQSTTEE"
     gene            610..>1349
                     /locus_tag="ACL73L_002074"
     CDS             610..>1349
                     /locus_tag="ACL73L_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772966.1"
                     /translation="MLRLVYYQFLHNKKQWLGVSPVIFVSSLVMGLAVNGVINVENNS
                     QVFVGLPDPKPIFMFPIVFGGVTLFFVLSNIINMLVEIFRDDYELLEVLGASLLQLSF
                     LVGGQIFIISSIISFIAYLCSIFVTSNYYYFLQYFFGENILPDIQFQTSAVGCIITVV
                     LISFLAFLSGCFYTFKKIRNRKSSKIRHVLSIVKRILLLAGFSVIWLLSLQQIFQDST
                     ILAKAQIIFNIVILDIVIIYQLSPFIQS"
BASE COUNT          428 a          182 c          262 g          477 t
ORIGIN      
        1 tagtggttct ggaaagtcaa ctttattaaa atgtatttct agtttgcttg aaccaacaag
       61 tggggaagta attttaaatg gaatcaaccc ctataaaatc agaaatgcaa aattgtcaag
      121 tataagacgt aacgaagtat cttttatatt tcaagcatac aatttaatac cttccctgcc
      181 ggtaatagaa aatatagcac ttcctttgcg attatcacaa aaaaaattaa ctattaaaaa
      241 tgtagaaaac ttactcaaaa gaatgaagtt taatgctggc ttaaacgatt ttgttggaac
      301 tctgtctggt ggagaacaac agaaggttgc tatagctaga gcggttattg ctgatagtga
      361 tataatattt gctgatgagc caactggggc tttagacagc gtttctcgtg aagtaatttt
      421 tgaattattg agagagttag taggggcggg taagtgtgta attatggtaa cgcacgatat
      481 agaattggcc tcgaaaactg atcgtgcatt aatattgaaa gatggaaaaa ttttcaaaga
      541 acttcataga cctagcgggg aagagttgta taaaatctta gaggtacaat caactacgga
      601 ggaatagaga tgctcagatt agtttactat caatttttac ataataagaa acaatggtta
      661 ggcgtttcac cagtaatttt tgtttcgagt ttagttatgg gattggctgt taatggggta
      721 attaatgttg agaataattc gcaagtattt gtcggacttc cagatcctaa accaattttt
      781 atgtttccga tagtattcgg aggagtaaca ttattctttg tgctctctaa cataataaat
      841 atgttggttg aaatttttag agatgattac gaacttctgg aggtgttggg agcaagtctc
      901 cttcaactat catttcttgt ggggggacag atttttatca tttcctcgat tattagtttt
      961 attgcatatc tatgctctat atttgttacg tcaaattatt attatttttt acaatacttt
     1021 ttcggggaaa atatattacc tgatatccaa tttcaaacga gtgctgttgg atgtatcatt
     1081 acagtagtgt tgatttcatt tttagccttt ctaagtggtt gcttttatac gttcaagaag
     1141 attagaaata ggaagagttc taaaataagg catgttcttt caatagtaaa aagaatactg
     1201 ctattggctg gttttagtgt tatctggttg ctttctttgc aacaaatttt ccaagatagc
     1261 acaattttag cgaaggccca gataatattc aatattgtaa tcttagatat tgtgattatt
     1321 tatcaattat ctccttttat acagtcatg
//
LOCUS       DBERDK010000143         1306 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.143, whole genome shotgun sequence.
ACCESSION   DBERDK010000143 DBERDK010000000
VERSION     DBERDK010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.143"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..>1306
                     /locus_tag="ACL73L_002075"
     CDS             <1..>1306
                     /locus_tag="ACL73L_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HGS0772967.1"
                     /translation="LRYRSNHWVPDSRPEEPSPQLTPEPSPSPQPAPNPQPAPSNPID
                     EKLVKEAVRKVGDGYVFEENGVSRYIPAKDLSAETAAGIDSKLAKQESLSHKLGTKKT
                     DLPSSDREFYNKAYDLLARIHQDLLDNKGRQVDFEALDNLLERLKDVSSDKVKLVEDI
                     LAFLAPIRHPERLGKPNAQITYTDDEIQVAKLAGKYTTEDGYIFDPRDITSDEGDAYV
                     TPHMTHSHWIKKDSLSEAERAAAQAYAKEKGLTPPSTDHQDSGNTEAKGAEAIYNRVK
                     AAKKVPLDRMPYNLQYTVEVKNGSLIIPHYDHYHNIKFEWFDEGLYEAPKGYTLEDLL
                     ATVKYYVEHPNERPHSDNGFGNASDHVRKNKADQDSKPDEDKEHDEVSEPTHPESDEK
                     ENHAGLNPSADNLYKPSTDTEETEEEAEDTTDEAEIPQVEHSV"
BASE COUNT          440 a          270 c          282 g          314 t
ORIGIN      
        1 cccttcgtta tcgttcaaac cattgggtac cagattcaag accagaagaa ccaagtccac
       61 aactgactcc agaacctagt ccaagtccgc aacctgcacc aaatcctcaa ccagctccaa
      121 gcaatccaat tgatgagaaa ttggtcaaag aagctgttcg aaaagtaggc gatggttatg
      181 tctttgagga gaatggagtt tctcgttata tcccagccaa ggatctttca gcagaaacag
      241 cagcaggcat tgatagcaaa ctggccaagc aggaaagttt atctcataag ctaggaacta
      301 agaaaactga cctcccatct agtgatcgag aattttacaa taaggcttat gacttactag
      361 caagaattca ccaagattta cttgataata aaggtcgaca agttgatttt gaggctttgg
      421 ataacctgtt ggaacgactc aaggatgtct caagtgataa agtcaagtta gtggaagata
      481 ttcttgcctt cttagctccg attcgtcatc cagaacgttt aggaaaacca aatgcgcaaa
      541 ttacctacac tgatgatgag attcaagtag ccaagttggc aggcaagtac acaacagaag
      601 acggttatat ctttgatcct cgtgatataa ccagtgatga gggggatgcc tatgtaactc
      661 cacatatgac ccatagccac tggattaaaa aagatagttt gtctgaagct gagagagcgg
      721 cagcccaggc ttatgctaaa gagaaaggtt tgacccctcc ttcgacagac catcaggatt
      781 caggaaatac tgaggcaaaa ggagcagaag ctatctacaa ccgcgtgaaa gcagctaaga
      841 aggtgccact tgatcgtatg ccttacaatc ttcaatatac tgtagaagtc aaaaacggta
      901 gtttaatcat acctcattat gaccattacc ataacatcaa atttgagtgg tttgacgaag
      961 gcctttatga ggcacctaag gggtatactc ttgaggatct tttggcgact gtcaagtact
     1021 atgtcgaaca tccaaacgaa cgtccgcatt cagataatgg ttttggtaac gctagcgacc
     1081 atgttcgtaa aaataaggca gaccaagaca gtaaacctga tgaagataag gaacatgatg
     1141 aagtaagtga gccaactcac cctgaatctg atgaaaaaga gaatcacgct ggtttaaatc
     1201 cttcagcaga taatctttat aaaccaagca ctgatacgga agagacagag gaagaagctg
     1261 aagataccac agatgaggct gaaattcctc aagtagagca ttctgt
//
LOCUS       DBERDK010000144         1259 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.144, whole genome shotgun sequence.
ACCESSION   DBERDK010000144 DBERDK010000000
VERSION     DBERDK010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.144"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..>1259)
                     /locus_tag="ACL73L_002076"
     CDS             complement(<1..>1259)
                     /locus_tag="ACL73L_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S74"
                     /protein_id="HGS0772968.1"
                     /translation="TALSGDLDVLKRTIANDIRPKQAQAEAEIAKQVEALSRTKNELA
                     GASTLLAQEAKRIELDSVARLEAFKSQTTSAQTALSGDLDALKRTIANDIRPKQAQAE
                     TEIAKQVEALSRTKNELAGVKSAQATYEETTTRRLSELTNLANGKASKSELTQTAEEL
                     ASKIASVQAGSSRNYFRNSRSRTFTTGGQAVYDYRTFIVPDFWKNSDRFKRDYVRISF
                     DVTFPVALVNDMPAMVHFSAHPWYAYRNLIFKGGTVERQHFEFTIDLSSSSEDYQTNN
                     VFIRFGTNYGFPAGLQVVIENAMLSVGNYFPAYQPAYEDQEDRVSVVESNFKQRADSL
                     EAGVSRLTEGLRTKADISSLNVTAENIRQSVKSLETDTQNKLNQKLSQAEFEVRAGSI
                     RQEILNATKDKASKSELTQTAEELSSK"
BASE COUNT          305 a          317 c          271 g          366 t
ORIGIN      
        1 atcttactag agagctcctc agctgtctgc gtgagttctg acttgctggc tttatccttg
       61 gttgcgttca ggatttcctg acggatagag ccagctcgca cctcaaattc agcctgactc
      121 aacttctgat ttagcttgtt ctgcgtgtct gtctcaaggc tcttcacaga ttgccggata
      181 ttttcagcag tcacattgag tgagctgata tccgctttgg ttctaaggcc ttcagtcaga
      241 cggcttacac cagcttcgag tgaatcagca cgctgcttaa agttggattc gactactgag
      301 acacggtctt cttggtcttc atacgctggt tgataggctg gaaaataatt accaaccgat
      361 aacatagcgt tctcaatgac gacctgcaga ccagcaggaa atccataatt agtaccaaaa
      421 cgaatgaaca cattattagt ctgatagtcc tcagaagaac tagacaagtc aatcgtaaac
      481 tcaaaatgtt ggcgttcgac agttccacct ttaaaaatta agtttctgta ggcataccat
      541 ggatgagcac taaaatgcac catagcaggc atgtcattta ctagggcgac agggaaagtc
      601 acatcaaaag atatgcgaac ataatcacgc ttgaacctgt cactgttctt ccagaaatca
      661 ggaactatga atgttcgata gtcgtatacc gcttgacctc ctgttgtgaa cgttcttgaa
      721 cgtgaattcc tgaagtaatt ccgtgaacta cctgcctgca cactcgctat cttactagcc
      781 agctcctcag ctgtttgcgt gagttctgac ttgctggctt taccattggc caagttggtc
      841 agttctgaca gtctacgagt cgtcgtctcc tcatacgtcg cttgcgctga cttcacgcca
      901 gccagttcat ttttagtccg gctaagtgct tcaacttgct tggcaatctc agtttcagcc
      961 tgtgcttgct tcggtcgaat atcattcgcg atagtccgtt tcagggcatc caagtcaccc
     1021 gacagagccg tttgtgcgct cgtagtctgc gacttaaacg cttcaagtct agcaacagaa
     1081 tccagctcaa tccgcttagc ttcctgtgca agcagggtac ttgcgccagc cagttcattt
     1141 ttagtccggc taagtgcttc aacttgcttg gcaatctcag cttcagcctg tgcttgcttc
     1201 ggtcgaatat cgtttgcgat agttcgtttt agaacatcca agtcacctga caaagccgt
//
LOCUS       DBERDK010000145         1238 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.146, whole genome shotgun sequence.
ACCESSION   DBERDK010000145 DBERDK010000000
VERSION     DBERDK010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.146"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..>1238
                     /locus_tag="ACL73L_002077"
     CDS             join(<1..332,332..>1238)
                     /locus_tag="ACL73L_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGS0772969.1"
                     /translation="MVKLIDRYGIEIVKKGKNRHYSSKLKQEMMDKALLEGCSQRSIS
                     LDYALPNQGMLSFWPAQYKKNGYTIVEKTRGRPAKMGRKRKKTWEEMTELERLQEENE
                     RLRTEVAYPKKVKRVRGKGRSPRARKAETVREMVSGGFRLDFLLETARLARSTYYYQL
                     KQLDGVDKDKEIKTEIQGIDNEHKGNYGYRRIHLELRNRGFVVNHKKVQRLMRILGLT
                     ARIRRKRKYSSYQGEIGKKAENLIQRQFEASRPMEKCYTDVTEFAIPNSTQKLYLSPV
                     LDGFNSEIIAYHLSTSPNLEQVKSMLEQAFTEKYYENTILHSDQGWQYQHDSYHRFLE
                     SKEIQASMSRKGNSPDNGMMESFFGILKSEMFYGYEKNFRSLENLEQAIVDYIDYYNN
                     KRIKVKLKGFSSVQYRTKSFG"
BASE COUNT          441 a          213 c          273 g          311 t
ORIGIN      
        1 catggtgaaa ttaattgacc gttatggcat agaaatcgtt aaaaaaggga agaatcgtca
       61 ctattcttct aaattgaaac aagaaatgat ggataaagcc ttactggaag gttgttcaca
      121 aagaagtatt agtcttgatt atgccctccc aaaccaagga atgctttcat tttggccggc
      181 acaatacaag aaaaacgggt atactattgt tgagaaaaca agagggagac cagctaaaat
      241 gggacgtaaa cggaagaaga cttgggaaga aatgacagaa ctagaacgac tccaggagga
      301 gaatgaacgc ttacggactg aggtggccta cctaaaaaag ttaaaagagt tagaggaaag
      361 ggacgaagcc ctagagcgag aaaggcagag acagttagag aaatggtttc aggaggattt
      421 cgactagatt ttcttcttga aacagcacgt ttagctcgct cgacttacta ttatcagttg
      481 aaacaactgg atggggttga taaagataaa gagattaaaa ccgaaattca gggcattgat
      541 aatgaacata aaggcaacta tggctatcgt cgaatccatt tagaactaag gaatcgtggt
      601 tttgtggtca atcataagaa agttcaacgg ttgatgagaa ttcttggttt aacggctcga
      661 attcgccgta aacggaagta ttcttcctac caaggagaga ttggcaagaa agcagagaat
      721 ctcattcaac gccagttcga agcatcgagg ccaatggaaa agtgctatac agatgtgaca
      781 gagtttgcca ttccaaatag cacacagaaa ctctatttat caccagtttt agatggtttt
      841 aacagtgaaa tcattgctta tcatctttct acttcgccaa acttagaaca agtgaagagt
      901 atgttagaac aggcctttac agagaaatac tatgagaata cgattctcca tagtgaccaa
      961 ggctggcaat accaacacga ttcttatcat cggttcctag agagtaagga aattcaagca
     1021 tccatgtcac gcaagggcaa cagcccagac aacggcatga tggaatcttt ctttggcatt
     1081 ttaaaatccg aaatgtttta tggctatgag aagaacttta gatctttaga aaaccttgaa
     1141 caagctattg tggactacat tgattactac aacaacaaac gaattaaggt aaaactaaaa
     1201 ggatttagtt ctgtgcagta cagaactaaa tcctttgg
//
LOCUS       DBERDK010000146         1201 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.147, whole genome shotgun sequence.
ACCESSION   DBERDK010000146 DBERDK010000000
VERSION     DBERDK010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.147"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            362..1201
                     /locus_tag="ACL73L_002078"
                     /pseudo
     CDS             362..1201
                     /locus_tag="ACL73L_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220488.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
BASE COUNT          453 a          130 c          216 g          402 t
ORIGIN      
        1 gtttaatgta gttgtttagt tttaaaaaat tatgcaaagc tccatataca acacttaaac
       61 gagattaaag aatggcgaaa tcccttgatg cgcaagagat tagctgtcct tttttatttt
      121 ttaagtgata aagtcggagc ttaggtatca aagcctatca aattaaacaa agaagtgatg
      181 tttagatatt ttgaaaaaaa ttaataagca gaaaactctc tattattttg ttgtagagag
      241 ttttttgtta ataaaatttt acaaaatgac atttatatat tgcattaagt tagatatatg
      301 atataatgtt gttaaaaaga ggcgcaactt tttaaactca ataagaatca aagagcaaac
      361 catgaatatt aatggaggaa taaaaaatgt aagttataaa tgtaagtaag cattatggtc
      421 attcaatcat tctcaaagat ataaattttg cacttaacaa gggtgaaatt gttggtctag
      481 tagggagaaa tggagttggt aagagtacgt tgatgaaaat tcttgttcag aataatcaac
      541 cgacttcagg taatattata agcagtgata atattgggta tttaatcgaa gaaccaaaat
      601 tatttttatc taaaacaggt ttagagaatt taaaatattt gtcaaattta tatggtgttg
      661 actacaatca agaaagattt agaagtttga tccaagagtt agatttgact cagtctatta
      721 ataaaaaagt aaagacctat tctttgggta caaaacaaaa attagctttg cttctaactc
      781 tcgttacgaa acctgatata ttgattttag atgaaccgac taatggttta gatattgaat
      841 catcacaaat agttttagcg gttctaaaaa aattagcttt aaatgaaaac gtgggaattt
      901 taatatcgag tcataaatta gaagacattg aagaaatttg tgagagggtt cttttcttgg
      961 agagcgggct tttgacattt caaaaagtag gaaaagatag tcataatttc ttgtttgaga
     1021 tagctttttc atcagctaca gatagagaca ttttcattac caaacaagaa tttggggata
     1081 ttgttcagga agagggactg agaattacta tgtctgggaa tattcaaagt agtgagcttt
     1141 ttaaattttt taacgaaaac tctattaaag tagttgattt tgaaactaaa aaagagacgc
     1201 t
//
LOCUS       DBERDK010000147         1186 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.148, whole genome shotgun sequence.
ACCESSION   DBERDK010000147 DBERDK010000000
VERSION     DBERDK010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.148"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            40..1065
                     /locus_tag="ACL73L_002079"
     CDS             40..1065
                     /locus_tag="ACL73L_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HGS0772970.1"
                     /translation="MKNKFFLIAILAMCIVFSACSSNSVKNEENTSKEHAPDKIVLDH
                     AFGQTILDKKPERVATIAWGNHDVALALGIVPVGFSKANYGVSADKGVLPWTEEKIKE
                     LNGKANLFDDLDGLNFEAISNSKPDVILAGYSGITKEDYDTLSKIAPVAAYKSKPWQT
                     LWRDMIKIDSKALGMEKEGDELIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAAD
                     TSKFWIYTSKDPRANYLTDLGLVFPESLKEFESEDSFAKEISAEEANKINDADVIITY
                     GDDKTLEALQKDPLLGKINAIKNGAVAVIPDNTPLAASCTPTPLSINYTIEEYLNLLG
                     NACKNAK"
     gene            1055..>1186
                     /locus_tag="ACL73L_002080"
     CDS             1055..>1186
                     /locus_tag="ACL73L_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HGS0772971.1"
                     /translation="MRNKKQINLGIIFVICLGLLITIFLSLKLGTKEINIRDFLAAFG
                     "
BASE COUNT          460 a          163 c          199 g          364 t
ORIGIN      
        1 atcgatacaa tagacttgaa atatgtttaa ggagttttta tgaaaaacaa attttttcta
       61 atagctattt tagctatgtg tatagttttt agcgcttgtt cttctaattc tgttaaaaat
      121 gaagaaaata cttctaaaga gcatgcgcct gataaaatag ttttagatca tgctttcggt
      181 caaactatat tagataaaaa acctgaaaga gttgcaacta ttgcttgggg aaatcatgat
      241 gtagcattag ctttaggaat agttcctgtt ggattttcaa aagcaaatta cggtgtaagt
      301 gctgataaag gagttttacc atggacagaa gaaaaaatca aagaactaaa tggtaaagct
      361 aacctatttg acgatttgga tggacttaac tttgaagcaa tatcaaattc taaaccagat
      421 gttatcttag caggttattc tggtataact aaagaagatt atgacactct atcaaaaatt
      481 gctcctgtag cagcatacaa atctaaacct tggcaaactt tatggagaga tatgattaaa
      541 attgattcaa aagccttagg tatggaaaaa gaaggtgatg agttaatcaa aaatactgaa
      601 gctcgtatat ccaaagaatt agaaaaacat ccagaaatca aaggaaaaat caaaggaaaa
      661 aaagtattat ttactatgat taatgctgca gatacatcaa aattctggat ttatactagc
      721 aaagatccaa gagcaaatta tttaacagat ttaggtctag ttttccctga atcattaaaa
      781 gaatttgaga gtgaagatag ttttgcaaag gaaatttctg cagaagaagc aaataagata
      841 aatgatgctg atgtaatcat aacttatggt gatgataaaa ctcttgaagc tttacaaaaa
      901 gatcctcttt taggtaaaat aaatgcaatt aaaaatggtg ccgttgctgt aattccagat
      961 aatacaccgt tagcagcctc atgcactcca acaccacttt caataaacta tactattgaa
     1021 gaatacctaa atcttttagg aaatgcatgc aaaaatgcga aataaaaaac aaataaacct
     1081 aggcataatt tttgtaatct gcctaggtct tcttattaca atatttttgt cattaaagct
     1141 tggaacaaaa gaaattaata tcagagattt tttagcagct tttgga
//
LOCUS       DBERDK010000148         1114 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.149, whole genome shotgun sequence.
ACCESSION   DBERDK010000148 DBERDK010000000
VERSION     DBERDK010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.149"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(8..81)
                     /locus_tag="ACL73L_002081"
     tRNA            complement(8..81)
                     /locus_tag="ACL73L_002081"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(45..47),aa:Ile,seq:cat)
     gene            complement(95..168)
                     /locus_tag="ACL73L_002082"
     tRNA            complement(95..168)
                     /locus_tag="ACL73L_002082"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(132..134),aa:Met,seq:cat)
     gene            complement(174..247)
                     /locus_tag="ACL73L_002083"
     tRNA            complement(174..247)
                     /locus_tag="ACL73L_002083"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(211..213),aa:Pro,seq:tgg)
     gene            complement(263..336)
                     /locus_tag="ACL73L_002084"
     tRNA            complement(263..336)
                     /locus_tag="ACL73L_002084"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(300..302),aa:Arg,seq:acg)
     gene            complement(346..431)
                     /locus_tag="ACL73L_002085"
     tRNA            complement(346..431)
                     /locus_tag="ACL73L_002085"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(395..397),aa:Leu,seq:taa)
     gene            complement(439..510)
                     /locus_tag="ACL73L_002086"
     tRNA            complement(439..510)
                     /locus_tag="ACL73L_002086"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(476..478),aa:Gly,seq:gcc)
     gene            complement(533..605)
                     /locus_tag="ACL73L_002087"
     tRNA            complement(533..605)
                     /locus_tag="ACL73L_002087"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(570..572),aa:Thr,seq:tgt)
     gene            complement(624..705)
                     /locus_tag="ACL73L_002088"
     tRNA            complement(624..705)
                     /locus_tag="ACL73L_002088"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(669..671),aa:Leu,seq:tag)
     gene            complement(711..783)
                     /locus_tag="ACL73L_002089"
     tRNA            complement(711..783)
                     /locus_tag="ACL73L_002089"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(748..750),aa:Lys,seq:ttt)
     gene            complement(808..880)
                     /locus_tag="ACL73L_002090"
     tRNA            complement(808..880)
                     /locus_tag="ACL73L_002090"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(845..847),aa:Asp,seq:gtc)
     gene            complement(883..955)
                     /locus_tag="ACL73L_002091"
     tRNA            complement(883..955)
                     /locus_tag="ACL73L_002091"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(920..922),aa:Val,seq:tac)
     gene            complement(960..1075)
                     /gene="rrf"
                     /locus_tag="ACL73L_002092"
     rRNA            complement(960..1075)
                     /gene="rrf"
                     /locus_tag="ACL73L_002092"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          288 a          320 c          249 g          257 t
ORIGIN      
        1 attcaaatgg accttgtagg acttgaacct acgaccactc ggttatgagc cgagagctct
       61 aaccagctga gctaaaggtc cgacaagatc attatagcgg cgaaggggat cgaacccccg
      121 acctcccggg tatgaaccgg acgctctagc cagctgagct acaccgccat gaatcgggaa
      181 gacaggattc gaacctgcga caccttggtc ccaaaccaag tactctacca agctgagcta
      241 cttcccgagt taaatagaaa aatgcaccct agaggagtcg aacctctaac cgcctgattc
      301 gtagtcaggt actctatcca gttgagctaa gggtgctcca tattatgccg aggaccggaa
      361 tcgaaccggt acgatcgtta ccaatcgcag gattttaagt cctgtgcgtc tgccagttcc
      421 gccaccccgg cctctctaag cgaacgacgg gattcgaacc cgcgaccccc accttggcaa
      481 ggtggtgttc taccactgaa ctacgttcgc actgttttct tctatctaaa aatgccggct
      541 acatgacttg aacacgcgac cctctgatta caaatcagat gctctaccaa ctgagctaag
      601 ccggctcatt tgttatatct taatgcgggt taagggactt gaacccccac gccgttaagc
      661 gccagatcct aaatctggtg cgtctgccaa ttccgccaaa cccgcatata tgacccgtac
      721 tgggctcgaa ccagtgaccc attgattaaa agtcaattgc tctaccaact gagctaacga
      781 gtctaaaata acttgcgtta tcttaaacgg tcccgacggg aatcgaaccc gcgatcttcg
      841 ccgtgacagg gcgacgtgat aaccgctaca ctacgggacc tttgggagtt aacgggatcg
      901 aaccgctgac cctctgcttg taaggcagat gctctcccag ctgagctaaa ctccctagag
      961 ctaagcgact tccctatctc acagggggca acccccaact acttccggcg ttctagggct
     1021 taacttctgt gttcggcatg ggtacaggtg tatctcctag gctatcgtca cttaactctg
     1081 agtaatacct actcaaaatt gaatatctat tcaa
//
LOCUS       DBERDK010000149         1077 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.150, whole genome shotgun sequence.
ACCESSION   DBERDK010000149 DBERDK010000000
VERSION     DBERDK010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.150"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            187..756
                     /locus_tag="ACL73L_002093"
                     /pseudo
     CDS             187..756
                     /locus_tag="ACL73L_002093"
                     /inference="COORDINATES: protein motif:HMM:NF021048.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
     gene            764..>1077
                     /locus_tag="ACL73L_002094"
     CDS             764..>1077
                     /locus_tag="ACL73L_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HGS0772972.1"
                     /translation="MTVLKGDNLEILKTIESSSIDLIYMDPPFFTQKTQKLSNNKNIM
                     YSFEDTWTSIEDYKEFLSVRLEECKRVLKNSGSIFVHCDKIANHHIRLILDNIFGADM
                     FQ"
BASE COUNT          452 a          130 c          173 g          322 t
ORIGIN      
        1 gaaatagcaa tattactaga acttttagaa acaatcaata tttaaaaaga aaaaagctca
       61 aagaatttat taaaccatgt tataatagat atagctataa ttttatttat aacaagagga
      121 tagaaatgac cgaattagaa agaaaaaatc gaaaaattag ctaagaaata ttctgataac
      181 ttaaacatca aagttcaaga gagagttcgt gaaatggcaa atgataataa gagccattat
      241 ttgatataca gagttttagg tatttcattt gaagaaggag aaaatatcga tttgtatcaa
      301 aataaaggtc gttttttata caaatatgct ggttcatttt tagaagaagc tgcagtacta
      361 tcctttaacg aaaaatttgg tacagaaaat acttaaaaag ttaacattcc taattctgaa
      421 agtacaaaac ctaagacttt tgaaattgat tgtttagtcg gagaaaaaca cgcatacgaa
      481 ataaaatggt gggatgcaac tacagatgga gaccatataa ctaaagaaca cactagaata
      541 aaagttattc ataacaaagg atatatacca attcggttaa tgttctacta tccaaataga
      601 actcaagcta taaaaattca gcaaacttta gaaacattgt ataacggtat tggagggaaa
      661 tattatggag attctgcctg ggaacattta agagcagtga ccggtattga tttacttagt
      721 attctaacag atattgcaaa taaaaaacag gggtaaaatc aaaatgacag tattaaaagg
      781 agataactta gaaatattaa aaactattga atcctcaagt attgatttaa tctatatgga
      841 ccctcctttc tttacacaga aaacccaaaa attatctaat aacaaaaata ttatgtattc
      901 attcgaagat acgtggactt cgattgagga ttacaaagaa tttttgtctg taagattaga
      961 agaatgcaaa agagtgctaa aaaatagtgg cagtattttc gttcattgtg ataaaattgc
     1021 aaatcatcat attagattaa ttttagataa tatctttgga gcagatatgt ttcaaag
//
LOCUS       DBERDK010000150         1073 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.151, whole genome shotgun sequence.
ACCESSION   DBERDK010000150 DBERDK010000000
VERSION     DBERDK010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.151"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            55..279
                     /locus_tag="ACL73L_002095"
     CDS             55..279
                     /locus_tag="ACL73L_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000269692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HGS0772973.1"
                     /translation="MIAGGLIGLLAGKITKKSSSMGIIANVFAGLVGASAGQSLLGSW
                     GSSIAGMALLPSIVGAAIVITVVSFFTGRK"
     gene            363..929
                     /gene="amaP"
                     /locus_tag="ACL73L_002096"
     CDS             363..929
                     /gene="amaP"
                     /locus_tag="ACL73L_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HGS0772974.1"
                     /translation="MSKAKKICFIIFCILILTIFLPVLIDYHQVSDLGIHLLSWRQNS
                     VVEFYLARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFV
                     RSLVSDHRLIKNPTVHVNLRKNKCFVHVEGKILPSDNIADRCQIIQNEITNGLKQFFG
                     IERQVKLEVAVKNYQPKPQNKKTVSRVK"
     gene            941..>1073
                     /locus_tag="ACL73L_002097"
     CDS             941..>1073
                     /locus_tag="ACL73L_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HGS0772975.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     "
BASE COUNT          345 a          151 c          213 g          364 t
ORIGIN      
        1 aaattgattt tttgatatta aaataaaaaa ggagggtagg cagtgttgtg atcaattatt
       61 gctggaggtc ttattggtct cttggcaggt aaaatcacta aaaaaagtag ttctatggga
      121 atcatcgcaa atgtattcgc tggtttagtc ggggcatctg caggacaatc tcttttaggt
      181 agttggggtt catcaatcgc tggaatggct ttgctcccat ctattgtagg tgcagcgatt
      241 gtgattactg tagtgtcatt ctttacaggt agaaagtaaa cttttcgcca gtaaagttag
      301 caaactattt ttaaatcaat gacgggaaaa atagtttaaa tgttaaatcg aaaggattgt
      361 atatgtcaaa agcaaagaaa atatgtttca ttattttctg tattttaatc ttgacaattt
      421 tccttcctgt tttgatagat tatcatcaag ttagtgatct aggtattcat ctacttagct
      481 ggagacagaa ctccgtagtt gaattctatc ttgctagata tgtcttttgg gggacagtgg
      541 ttctatcaac tttagtttta ttatccattt tagttgtgat gttttatcct aaacgttact
      601 tggaaatcca acttgaaact aaaaacgata cattaaaatt aaagaattcg gcaatcgaag
      661 gttttgttag aagtttggtg agtgatcata gattgatcaa gaacccaact gttcatgtaa
      721 atttacgaaa aaataaatgt ttcgttcatg tagaaggtaa aattcttcct tcagacaaca
      781 tcgctgacag atgccaaata attcaaaatg aaataactaa tggattgaag cagttttttg
      841 gtattgagcg tcaagtaaaa cttgaagttg cagtaaaaaa ttaccaacca aaacctcaaa
      901 acaaaaagac tgttagtcgt gtgaagtaag gaagtaaaaa atggaatggc ttaaacaata
      961 tcgatatcca attatcgctg gtctcatagg cgtatttctg gcttgtttga ttgtctcctt
     1021 tggcttcttc aaaacaatat ttgtattgat tttaggagca ctgggagttg cag
//
LOCUS       DBERDK010000151         1063 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.152, whole genome shotgun sequence.
ACCESSION   DBERDK010000151 DBERDK010000000
VERSION     DBERDK010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.152"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..619
                     /locus_tag="ACL73L_002098"
     CDS             <1..619
                     /locus_tag="ACL73L_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGS0772976.1"
                     /translation="CYKYGDKEILKNLNFCFQRNEKYLILGESGSGKSTLLKLLNGFL
                     RDYSGELRFCGDDIKKTSYLNMVSNVLYVDQKAYLFEGTIRDNILLEENYTDEEILQS
                     LEQVGLSVKDFPNNILDYYVGDDGRLLSGGQKQKITLARGLIRNKKIVLIDEGTSAID
                     RRTSLAIERKILDREDLTVIIVTHAPHPELKQYFTKIYQFPKDFI"
BASE COUNT          390 a          111 c          203 g          359 t
ORIGIN      
        1 ttgctataag tatggagata aagaaatatt aaaaaactta aatttttgtt ttcaacgtaa
       61 tgaaaagtat ttaattttag gtgaaagtgg aagcgggaaa tctacattat taaaattatt
      121 gaatggcttt ttgagagatt atagtggaga attgcgattc tgtggggatg atataaaaaa
      181 aacctcctat ttaaatatgg tttcgaatgt tctatatgta gatcaaaaag cttatttgtt
      241 tgaaggtacg attagagata atattttatt ggaagaaaat tatactgatg aagaaatact
      301 acagtcttta gaacaagttg gtttgagtgt aaaagatttt cctaataaca ttttagatta
      361 ttatgttggt gatgatggga gattactgtc aggagggcag aaacaaaaaa ttactttagc
      421 tagaggccta attagaaata agaaaatagt attaattgac gagggaactt ctgctatcga
      481 taggagaact tcgttagcga ttgaacgtaa gatattagat agagaggatt tgactgtcat
      541 tattgttacc catgctccgc atccggaact taaacaatat tttactaaga tatatcaatt
      601 tccaaaggat tttatttaag aatatagggg agggaaacat gagattctat tgtgaagctt
      661 atgaggtaat tactgaagag aaaaaagtat actttaatga tattgagtta gaagttaagt
      721 attcctctgt cgaagaattg tttataatat gtgagaaatt attagataag aaaaaagtat
      781 cattttttat gtagatgaga aacccttaag gtatctgtta tttgactata tatttctact
      841 agttctagct aaaaaaatat cccaatctta tgaactgcac cccaaaagtt agatttattc
      901 tgtctaactt ttggggtgca gttcattttg catagttttg gcattcatcc tagatatcca
      961 tataaaatgt ctgagattgt tgaaatgtct aaaaaaatat cagatctaaa cgataaaaaa
     1021 gaatttcaca aaaagaactg tgagttgagg tttcaagata gtg
//
LOCUS       DBERDK010000152         1053 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.153, whole genome shotgun sequence.
ACCESSION   DBERDK010000152 DBERDK010000000
VERSION     DBERDK010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.153"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            63..227
                     /locus_tag="ACL73L_002099"
     CDS             63..227
                     /locus_tag="ACL73L_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HGS0772977.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKEKKDKFLSILTSLAGIALV
                     LAAVWLGWPK"
     gene            306..1034
                     /gene="yaaA"
                     /locus_tag="ACL73L_002100"
     CDS             306..1034
                     /gene="yaaA"
                     /locus_tag="ACL73L_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HGS0772978.1"
                     /translation="MKILIPTAKEMNTDLPSIEAIPLKPESQTVLDALALYSASQLES
                     FYKVSAEKAAEEFQNIQALKRQTAQHYPALKLFDGLMYRNIKRDKLTEAEQDYLENHV
                     FITSALYGVVPALSPMAPHRLDFLMKLKVAGKTLKSHWKAVYDEALKKEEVIFSLLSS
                     EFETVFSKEIRAKMVTFKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
BASE COUNT          334 a          186 c          230 g          303 t
ORIGIN      
        1 aaggtttatt taataaatat atgttataat aaggtaaatt aagcctaatg gaggacaaag
       61 aaatgggttt ttatttgatg gttgcttcaa tgctactcgg actgctcgct ttgaagatag
      121 gtttttctca gttcaaggag aaaaaagata aattcttatc tatcttaaca agtttagctg
      181 gcatagccct tgttctcgcg gctgtctggt taggatggcc caaataaata gaaaaacctc
      241 tgtcaaaccg aggtttttca aatgaatttc ttggttgtgg ctaaaaaata tgctacacta
      301 tcaatatgaa aattttaatc ccaacagcaa aagaaatgaa cacagacttg ccaagtatcg
      361 aggcaattcc tttaaaacca gaaagtcaga ccgtgcttga tgccttggct ctctattctg
      421 ccagtcaatt ggagagtttc tacaaggtat cagctgagaa agcggcggaa gaatttcaaa
      481 atatccaagc tttgaaaagg caaactgctc aacactatcc agccttgaaa ctttttgatg
      541 ggcttatgta ccgcaacatt aagagagata agctgaccga ggcggaacaa gattatcttg
      601 aaaatcatgt cttcattacc tcggctttgt acggtgttgt tccagccttg tcacccatgg
      661 ctcctcaccg tttggatttt ttgatgaaat taaaagtcgc tggtaagact ttgaagagcc
      721 attggaaggc agtctatgat gaagctctga agaaggaaga agtgattttc tctctcttgt
      781 catcagagtt tgagactgta ttttctaagg aaatcagagc aaagatggtg accttcaaat
      841 tcatggagga tagaggcggt cagctgaaga ttcactcaac tatctccaag aaagcgcgcg
      901 gggcctttct aacagcttta atagaaaatc aagtacaaac tgtgggggaa gcacgtcgct
      961 tgaactttgc tggatttgtt taccgagaag atttgtcaca accacaggga ttggtttttg
     1021 taaaggaagt atagaaatca gataagagaa aag
//
LOCUS       DBERDK010000153         1026 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.154, whole genome shotgun sequence.
ACCESSION   DBERDK010000153 DBERDK010000000
VERSION     DBERDK010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.154"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..145)
                     /locus_tag="ACL73L_002101"
     CDS             complement(<1..145)
                     /locus_tag="ACL73L_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HGS0772979.1"
                     /translation="MNIAVIGLGHVGLAYALLFASKYKVVAYDIDSVKINNLKKGILP
                     SKNE"
     gene            complement(158..907)
                     /locus_tag="ACL73L_002102"
     CDS             complement(158..907)
                     /locus_tag="ACL73L_002102"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HGS0772980.1"
                     /translation="MSNNTKYIFLSPHLDDAIFSCGDYISKLTSEGEIVLVITIFSGY
                     PLSQQLQPSAKQFHKLCNLGKYPIEERKKEDRLACERLQCDFRHLSYYECLYRKDRNG
                     NFLYRHIYSELKNEDTLKNDIIKELLMHLDDKCVVYCPLSLGDHVDHVFVNSIGRALE
                     FMRYKVIYYEDFPYVSDSSMVSYMGKTKELKMYQEELDEKHYIDRISSILCYKSQILI
                     IWKSVEKLLNNIKELYLRNGAAYSIIFWIKK"
BASE COUNT          360 a          194 c          112 g          360 t
ORIGIN      
        1 cttcattttt agatggaaga atgccctttt ttaaattatt tatttttaca gaatctatat
       61 catatgcaac aactttatat ttagatgcaa atagtaacgc gtaggccagc ccaacatgcc
      121 ccaaaccaat tactgctata ttcataaaac tacttcctta tttcttaatc caaaatataa
      181 tagaataagc tgccccattc cttaaataca actctttaat attgtttaaa agtttttcaa
      241 ctgatttcca gattatcaaa atctgagatt tatagcacaa tattgatgat attctatcaa
      301 tataatgttt ttcatcgagt tcctcttgat acatttttaa ttctttagtt tttcccatat
      361 aactaaccat actactatca cttacatatg ggaaatcctc ataatatatt actttataac
      421 gcataaattc aagcgccctt ccaatactat tcacaaaaac atgatcaaca tggtcaccaa
      481 gtgaaagcgg acaatatacg acacatttgt cgtctaaatg cattaacagc tcttttatga
      541 tatcattctt taatgtgtcc tcatttttta attcactata gatatgacgg tatagaaaat
      601 tgccatttct atctttccta tagagacatt catagtacga taagtgtcta aaatcacatt
      661 gtagacgttc acaagctaac ctatcttctt tcttcctttc ttcaatcgga tatttcccaa
      721 ggttacacaa cttatgaaat tgcttagcag agggctgtag ctgttgactc aaagggtaac
      781 cagaaaatat agtaataaca agtacaattt ctccttctga agttaatttt gaaatataat
      841 caccacagga aaaaattgcg tcatctaaat gtggagataa aaagatatac ttagtattgt
      901 tactcataac cattccctct acaatttatc taaaaactca ctaagtgtct gattaaattc
      961 cacatcatca aaaaaattca ccttattctt aataatgaat atttcgttaa ataaacatat
     1021 atataa
//
LOCUS       DBERDK010000154         1018 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.155, whole genome shotgun sequence.
ACCESSION   DBERDK010000154 DBERDK010000000
VERSION     DBERDK010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.155"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..356)
                     /locus_tag="ACL73L_002103"
     CDS             complement(<1..356)
                     /locus_tag="ACL73L_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772981.1"
                     /translation="MRKSGFLIYYVLFCIVCFLLGLLLSFILKNETFATIPPKLELQF
                     TLTVLQANSTNFLLYLFCFPISPLLQLFDLLSLGIQLGISGQMIGIVATIKGILPHAL
                     IEIPNFIWYQGMSQYL"
     gene            complement(353..>1018)
                     /locus_tag="ACL73L_002104"
     CDS             complement(353..>1018)
                     /locus_tag="ACL73L_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772982.1"
                     /translation="GQVVIYVILLNYFILVMGILDNHASNFYFKSSRMSQKRFRSSLF
                     ILLRFCWLNKGLTFSFILISILLSYLFWYMMTMLSIDDTKIPILFLYTSIYITLMEAI
                     IGNHKSSIGVDLALLQLSALNHSKMNFFFKNAQLMMLTHLISFLQFSLLLCLIPVTSY
                     SNDIKSILLNLLLIPFNYFIAYVYYKKALFLIKGTVSLLRWSFLVLYCFLIIFVILVG
                     GIR"
BASE COUNT          433 a          158 c          124 g          303 t
ORIGIN      
        1 aaaagatact gactcatacc ttgataccaa ataaaattcg gtatttcaat aagagcatga
       61 ggtaatattc cttttattgt agctactatt ccaatcatct gaccagatat acccaattgt
      121 attcctaaag atagcaaatc aaaaagttgc aataagggcg aaatcggaaa acagaataga
      181 tatagcaaaa aatttgtaga atttgcctgt aaaactgtca atgtaaattg taattccagt
      241 ttcggaggta tagtagcaaa agtctcattt tttaatataa agcttaaaag taaacctagt
      301 aaaaaacaga ctatacaaaa taacacatag taaatcagaa aaccactttt tctcatctaa
      361 tgccccctac taaaattaca aatattatca gaaaacaata taaaaccaga aaactccatc
      421 tcaataaact aactgtacct ttaattaaaa atagagcttt cttataatag acataggcta
      481 taaaataatt gaatggaatc aataacaaat tcaacaaaat actttttata tcattactat
      541 aacttgtcac cggaatcaaa cataacaaca atgaaaattg taagaaagag attaaatgcg
      601 tcaacatcat tagttgtgca ttcttaaaaa agaaattcat tttagaatga ttaagagcag
      661 ataattgaag taaagctaga tctaccccaa ttgaggattt atgattccct attattgcct
      721 ccattaatgt aatatatatt gaggtataaa gaaataatat tggtattttt gtgtcatcaa
      781 ttgataacat cgtcatcata taccaaaata gataggaaag aagaatacta atcaaaataa
      841 aagaaaaagt taacccttta tttaaccaac aaaaacgaag caatataaat agactgctac
      901 gaaatctttt ttgactcatt cgactgcttt taaaatagaa attactcgca tgattatcta
      961 atatccccat gactaaaata aaataattga gtagtatgac ataaattaca acttgtcc
//
LOCUS       DBERDK010000155         1003 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.156, whole genome shotgun sequence.
ACCESSION   DBERDK010000155 DBERDK010000000
VERSION     DBERDK010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.156"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(47..1003)
                     /gene="ltrA"
                     /locus_tag="ACL73L_002105"
                     /pseudo
     CDS             complement(47..1003)
                     /gene="ltrA"
                     /locus_tag="ACL73L_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT          267 a          233 c          165 g          338 t
ORIGIN      
        1 tttgactatc ctgatcgatt tgttctattc ttatttcatt ttactataat tgatagtggt
       61 cgccccagcc agatacctta tctgctatcc atttaggaac ccctaactta agcaatcccc
      121 ataatcgtct cgatttcttc ttccattgct tccagataat cactcgtagg cgagtatgca
      181 aacgctcatc tatgctggtg actatacttt tcatatttcc caatgagaaa tagtttatcc
      241 atcctcgaat agacaaattc agttgctcaa tacgtcttgt taggtctata ctccatttcc
      301 tctgtgttag tttcttcaat ttaagcttaa atctccaaac actatcttga tgtggacgac
      361 ttttccaacc atctgatgat ttccagaacc cgaaacctag atatttcaac tctcttggtc
      421 tggtaatctt agtcttggtc atgtttactt tcagacctag ccgtttctca ataaaacgac
      481 tgactgaata catcacacgc ttagcggctg cctcgcttcc gaccgtaatc acacaatcgt
      541 ctgcgtagcg aacaaatcga agccctctct tttctaattc cttgtccaat tcattaagca
      601 tgatattgga taagagagga gataaatttc ctccctgtgg cgtgccaact agcgttttat
      661 aacgttgacc gttaatgata acacctgaat gaagatattt acgaatcaag gattccgtat
      721 ctccgtcttc gataatgtta tgtactaagg acatcaatct atcttgagga actgtatcga
      781 aaaatttctc taggtctatg tccactatcc actcatagcc gtcatttaag tactctaaga
      841 gcttcatgat ggctttttca catgacctat ttggtctgaa tccatagctc gtatctgaga
      901 aatggggttc acaaataggg ctaataactt gaacaatggc ctgttgaatc attctatcca
      961 taactgttgg aattcctagt tgacggatgc ctccgtctgg ttt
//
LOCUS       DBERDK010000156          932 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.157, whole genome shotgun sequence.
ACCESSION   DBERDK010000156 DBERDK010000000
VERSION     DBERDK010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.157"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..>932
                     /locus_tag="ACL73L_002106"
     CDS             <1..>932
                     /locus_tag="ACL73L_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HGS0772983.1"
                     /translation="DFNFSKMGSEEAKFLIFPELPRGGELSLTARQSYDSQLRYSMLP
                     RVEDIMKKIVNQLREHLKILDIELSDLSYLSEGDQFILKELSSHVKINIKITPIKKIR
                     YVYEDIFLNQSAEELHELGLKYLSAGDLWASKKIFTYLVNKYDNRRDVFKLATTYNML
                     LETEKAEYYYNLYKHDGKVDHIVAANYMLAMLYARHHPKYLQDDQKAIVLLNEAYDLI
                     KDKVTLESIFNRNGYALLLFKMGKVEETIILLKRCLNQIKQLDNSSSSVVLHESVLWF
                     NLYQCYVTIGDNTNSRKTFYILKNMDPLFIEYYL"
BASE COUNT          354 a          117 c          152 g          309 t
ORIGIN      
        1 tggattttaa tttctcaaaa atgggtagcg aagaagctaa atttttaatt tttccagagt
       61 tacctagagg aggtgagcta agtttaactg ctcgtcaaag ttatgatagc cagttaaggt
      121 attcgatgtt acctagagtt gaggacatca tgaagaaaat tgtaaatcaa cttagagaac
      181 atttaaaaat tttagacatt gagctaagtg atttaagtta tttatcagag ggtgatcaat
      241 tcatattaaa ggaactatca agtcatgtaa aaataaatat taaaattact ccaataaaaa
      301 aaatacgtta tgtatatgaa gatatctttc tcaatcaatc agcagaggaa ttacatgaat
      361 taggattaaa atatttatca gcaggtgatt tatgggcttc taaaaaaata tttacttact
      421 tagtaaataa atatgataat agacgagatg ttttcaaatt agctacaact tataatatgt
      481 tattggaaac agaaaaagca gaatactact acaacctata caaacatgat ggaaaagttg
      541 accatattgt ggcagcaaac tatatgcttg caatgctcta tgcacgccat catcctaaat
      601 accttcaaga tgatcaaaaa gctattgtat tattgaatga agcgtatgat ttaattaaag
      661 ataaagtgac attagagagt atttttaatc gaaatgggta tgccctacta ttatttaaaa
      721 tgggcaaagt agaggaaaca atcatattat taaaacgttg tttaaatcaa ataaaacaat
      781 tagataactc tagttcttcg gttgttcttc atgaaagtgt actttggttt aatttatatc
      841 agtgctatgt cactataggt gacaatacta attctcggaa aactttttat attttgaaaa
      901 atatggatcc gctttttatt gagtactatc ta
//
LOCUS       DBERDK010000157          919 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.158, whole genome shotgun sequence.
ACCESSION   DBERDK010000157 DBERDK010000000
VERSION     DBERDK010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..919
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.158"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..>919)
                     /locus_tag="ACL73L_002107"
     CDS             complement(<1..>919)
                     /locus_tag="ACL73L_002107"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772984.1"
                     /translation="ADSDALVEADALVDAEAEALVLAEAEALVDAEADALVDADSEAD
                     VLAEADVLVDADSEADVLAEADVLVDADSDALVDAEAEALVDADAEALVLAEADALVD
                     ADSDAEILAEADALVLAEADALVEAEADAEVLALAEADALVDAEAEALVDAEADALVD
                     AEADALVDADSEADVLAEADALVDADSDALVDAEAEALVDAEAEVLVLAEADALVLAE
                     ADALVDAEADALVLAEAEALVDAEAEALVLAEAEALVDADSEALVDAETEALVDAEAD
                     ALVLAEAEALVLAEAEALVDADSDADVLAE"
BASE COUNT          221 a          294 c          215 g          189 t
ORIGIN      
        1 cttcagcaag cacatcagca tctgaatcag cgtcaaccag tgcttcggct tcagcaagta
       61 ccagtgcttc agcttcagca agtaccagtg cgtcggcttc agcatcaacg agtgcttcag
      121 tctcagcgtc aaccagtgcc tctgaatccg catcaacaag tgcctcggct tcagcaagta
      181 ccagtgcctc ggcttcggca tcaacaagtg cctcagcctc agcaagtaca agtgcgtcag
      241 cttccgcatc aacgagtgcg tccgcttcag caagtactag tgcatcagct tcagcaagta
      301 ctagcacctc agcctcagcg tcaacaagtg cttcggcttc agcgtcaacg agtgcgtctg
      361 agtcagcatc aacgagtgcg tcagcctcag caagcacatc agcttctgaa tctgcatcaa
      421 ccagtgcgtc agcttcagcg tcaaccagtg cgtcggcttc agcgtcgaca agtgcttcgg
      481 cttcagcatc aacgagtgcg tcggcctcag caagcgcaag tacctcagcg tcagcttccg
      541 cctcaaccag tgcgtcggct tcagcaagca caagtgcgtc agcctcagca agtatctcag
      601 cgtctgaatc ggcatcaacg agtgcgtccg cttcagcaag tactagcgcc tcagcatcag
      661 cgtcaacaag tgcttcggct tcagcgtcaa cgagtgcgtc tgagtcagca tcaacgagta
      721 cgtcagcctc agcaagcaca tcagcttctg aatctgcatc aacgagtacg tcagcctcag
      781 caagcacatc agcttctgaa tctgcatcaa ccagtgcgtc agcctcagca tcgacaagcg
      841 cctcagcttc agcaagtacc agtgcttcag cctcagcgtc gacaagtgcg tcggcctcaa
      901 ccagtgcatc tgaatcggc
//
LOCUS       DBERDK010000158          886 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.159, whole genome shotgun sequence.
ACCESSION   DBERDK010000158 DBERDK010000000
VERSION     DBERDK010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..886
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.159"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..>886)
                     /locus_tag="ACL73L_002108"
     CDS             complement(<1..>886)
                     /locus_tag="ACL73L_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HGS0772985.1"
                     /translation="LLLIYAATINALVLNELIAMNLERFLKLSIYQMIVWCGIIFLDW
                     VVKNYQVEVIQEFNLEIRNRVATDISNSTYQEFHSKSSGTYLSWLNNDVQTLNDQAFK
                     QLFLVIKGISGTIFAVVTLNHYHWSLTVATLFSLMIMLLVPKIFASKMREVSLNLTSQ
                     NEAFLKSSETILNGFDVLASLNLLYVLPKKIKEAGILLKMVIQRKTTVETLAGAISFF
                     LNIFFQISLVFLTGYLAIKGIVKIGTIEAIGALTGVIFTALGELGGQLSSIIGTKPIF
                     LKLYSINPIESNKMNDIEP"
BASE COUNT          313 a          157 c          123 g          293 t
ORIGIN      
        1 ttggttcgat atcattcatt ttatttgact caattggatt aattgaatac aattttaaaa
       61 aaataggctt cgtaccaata atagaggata attgacctcc taattcacct agcgctgtaa
      121 aaataacacc tgttagtgct cctattgctt caatagtacc aattttcact attcctttta
      181 ttgcaagata gcctgttaaa aaaacgagag atatctgaaa aaaaatattg agaaagaagc
      241 taatagcgcc tgctaacgtt tctacagttg tctttctttg tataaccatc tttaataaaa
      301 ttcctgcttc tttaattttc ttaggcaata catataaaag attcaaggac gctaacacat
      361 caaatccatt caatatagtc tcactagatt ttaaaaaagc ttcattttgg ctagttaaat
      421 ttagactaac ttctcgcatt ttcgatgcaa agatttttgg tacaagtagc ataatcatta
      481 atgaaaacaa ggtggctaca gtcaatgacc aatgatagtg attaagagtc acaactgcaa
      541 atatagtacc agaaattcct tttattacta aaaaaagttg tttaaaagcc tgatcattta
      601 aagtctgaac atcattattt agccacgaaa gatatgttcc tgatgattta ctatgaaatt
      661 cttgataggt agagttagag atgtctgtgg caactctatt tcgaatctct agattaaact
      721 cttggatcac ttcaacctga taatttttca ctacccagtc aaggaatatt atcccacacc
      781 agacaatcat ttggtagatt gacaatttca aaaaccgctc taaattcatc gcaattaatt
      841 cattcaacac cagagcatta atagttgctg cataaattag caataa
//
LOCUS       DBERDK010000159          867 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.160, whole genome shotgun sequence.
ACCESSION   DBERDK010000159 DBERDK010000000
VERSION     DBERDK010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.160"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            51..698
                     /locus_tag="ACL73L_002109"
     CDS             51..698
                     /locus_tag="ACL73L_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HGS0772986.1"
                     /translation="MDFEYFYNREAERFNFLKVPEILVDREEFRGLSAEAIILYSILL
                     KRTGMSFKNNWIDKEGRVFIYFTVEEIMKRRNISKPTAIKTLDELDVKKGIGLIERVR
                     LGLGKPNIIYVKDFMSIFQVKENDLQKSKNLTSEVKDFNLRSKENELQEVKNLDSNYI
                     ENNKSKYSKREYSFGENGLGTFQNVFLAAEDISDLQIIMNSQLENYIRLPAKLES"
     gene            complement(687..>867)
                     /locus_tag="ACL73L_002110"
     CDS             complement(687..>867)
                     /locus_tag="ACL73L_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HGS0772987.1"
                     /translation="TVEDKACNHALSKERSKVENIFAKVKTFKMFSTTYRNHRKRFGL
                     RMNLSAGIINHELGF"
BASE COUNT          321 a          123 c          160 g          263 t
ORIGIN      
        1 aaaactatcg tcttttcttt gcaaattttt aagaagggag gtgatcttgc atggactttg
       61 aatattttta taacagagaa gcggaaagat ttaacttctt aaaagtaccg gagatattag
      121 ttgatagaga agaatttcgg ggcttatcag cagaagcaat tatcctttat tccatacttc
      181 ttaaacggac aggaatgtca tttaagaata actggataga caaggaaggc agagtattta
      241 tctattttac tgtcgaagaa attatgaaaa gaagaaatat ctcaaagcca actgccataa
      301 aaacattaga tgagcttgat gtaaaaaaag gaataggact gatcgaaaga gtaaggcttg
      361 gacttggtaa gccgaacatc atttatgtta aagactttat gagtatattt caggtaaaag
      421 aaaatgactt acagaagtca aaaaacttaa cttcagaagt aaaagatttt aacctcagaa
      481 gtaaagaaaa tgaacttcaa gaggttaaga accttgactc taactatata gagaataata
      541 agagtaagta tagtaagaga gaatatagtt ttggtgaaaa cggacttgga acatttcaaa
      601 atgtgttttt agctgctgaa gatatatcgg atttacaaat cataatgaac tcacagcttg
      661 agaattacat tagacttcct gcaaaactag aatcctagtt catgattgat aataccagca
      721 ctcaaattca ttcgtaatcc gaagcgttta cgatgatttc gataggttgt tgaaaacatt
      781 ttaaacgttt ttactttggc aaagatattc tcaaccttgc ttctctcctt agatagcgca
      841 tgattacagg ctttatcttc aactgtt
//
LOCUS       DBERDK010000160          839 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.161, whole genome shotgun sequence.
ACCESSION   DBERDK010000160 DBERDK010000000
VERSION     DBERDK010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.161"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..381
                     /locus_tag="ACL73L_002111"
     CDS             <1..381
                     /locus_tag="ACL73L_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGS0772988.1"
                     /translation="NLSTSPNLEQVQTMLEQSFTEKHYENTILHSDQGWQYQHDSYHQ
                     FLEGKGIQASISRKGNSPDNGMMESFFGILKSEMFYGYEKTFKSLNRLEQAIVDYIDY
                     YNNKRIKVKLKGLSPVQYRTKSFG"
     gene            complement(560..>839)
                     /locus_tag="ACL73L_002112"
     CDS             complement(560..>839)
                     /locus_tag="ACL73L_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HGS0772989.1"
                     /translation="PQPEGKKPSVPAQPGTEDKKLSAPKPDMQPSPQPEGKKPSVPEI
                     NQEKEKAKLAVATEKKLPSTGVASNLVLEIIGLLGLIGTSFIAMKRRK"
BASE COUNT          257 a          157 c          149 g          276 t
ORIGIN      
        1 aatctttcaa cttcacccaa tttagaacaa gtacaaacaa tgttggaaca gtcattcaca
       61 gagaagcact acgagaatac gattctccat agtgaccaag gctggcaata tcaacacgat
      121 tcttatcatc agtttcttga gggtaaggga attcaagcat ccatttcacg taagggtaac
      181 agcccagaca atggtatgat ggagtccttt tttgggattc tgaaatcgga aatgttttat
      241 ggctatgaga aaacatttaa atcacttaat cgattggaac aagctattgt agactatatt
      301 gattactaca acaataaacg aattaaggta aaactaaaag gacttagccc tgtgcaatac
      361 agaactaaat ccttcggata aattaattgt ctaacttttg cggttcacta gccttctctt
      421 tttatatctt agattcatct ctatatgaaa tgacttataa ttattagata ccaattataa
      481 gtaaaatctt tgggatcttc aatggttaac caatcaaatg ctaaatatcg aaagtggata
      541 tttttaaaaa actgaatcat tattttcttc ttttcatcgc gatgaatgaa gttccaatca
      601 aaccaaggag accaatgatc tcaagaacta gattagacgc cactcctgta gatggcaatt
      661 tcttctctgt tgctacagca agcttagctt tttctttctc ctgattaatc tctggtacac
      721 ttggtttctt accctctggt tgagggcttg gttgcatatc tggttttgga gcagatagct
      781 ttttatcctc agtaccgggt tgtgccggta cacttggttt cttgccttct ggttgagga
//
LOCUS       DBERDK010000161          807 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.162, whole genome shotgun sequence.
ACCESSION   DBERDK010000161 DBERDK010000000
VERSION     DBERDK010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..807
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.162"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(138..>807)
                     /gene="galE"
                     /locus_tag="ACL73L_002113"
     CDS             complement(138..>807)
                     /gene="galE"
                     /locus_tag="ACL73L_002113"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HGS0772990.1"
                     /translation="NFVFSSSATVYESTPIMPFYETNPLKASNPYGRTKQYIEVLLND
                     LFISNSNWKIVCLRYFNPLGAHESGDLGEDPNGIPNNLVPYITQVAIGKLPYLNIFGV
                     DYSTPDGTCIRDYVHVNDLAYGHRKALEYIFNTDEGLYEVINLGSGVGFSVFEILHSL
                     ESVIGSYIPYKITSRRAGDMDVSIADISKAEELLGWKPRYDIMKMCQDTWKWQQKHPN
                     GYDD"
BASE COUNT          254 a          164 c          103 g          286 t
ORIGIN      
        1 ataatcttca tatttttttc tttgaacctc aggtgcaata attactacat ttctgttata
       61 cagatatttt agtttaacaa cccgtgcaga atatctaaat aatactatat gcatgttaat
      121 cccaatcctt tcaattatta gtcatcatat ccatttggat gtttctgttg ccatttccac
      181 gtatcttggc acattttcat aatatcatac cttggcttcc atcccagtag ctcttctgct
      241 tttgaaatat ctgcaattga aacgtccata tctcctgctc tccttgaagt tatcttatat
      301 ggaatatacg agcctatgac actctctaag ctgtgtagga tttcaaatac actaaaacca
      361 acaccacttc ctaaattgat tacttcatat aaaccttcat ctgtattaaa aatatactct
      421 aatgcttttc tgtgtccgta tgctaaatcg ttaacgtgaa catagtccct tatacaagtc
      481 ccatcaggag tactgtaatc aactccgaat atatttaaat atggtaattt tccaattgca
      541 acttgtgtaa tgtaaggaac aagattattc ggaattccat ttggatcctc tcctaaatca
      601 ccactttcat gtgcacccaa tggattaaaa tacctcagac aaacaatttt ccaatttgaa
      661 ttcgaaataa acaaatcatt taaaagtact tctatatact gttttgttct cccatacggg
      721 tttgaagctt ttaatggatt agtctcatag aatggcatga taggagtact ttcgtataca
      781 gtagctgatg aactaaacac aaaatta
//
LOCUS       DBERDK010000162          782 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.163, whole genome shotgun sequence.
ACCESSION   DBERDK010000162 DBERDK010000000
VERSION     DBERDK010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..782
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.163"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            63..593
                     /locus_tag="ACL73L_002114"
     CDS             63..593
                     /locus_tag="ACL73L_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772991.1"
                     /translation="MIINTLGPTATDCFYAINRIKLKVDVIVLYDSFDSIVNCFDKLK
                     GQYILLPVAFESSIKNYSWKDFNFEYHKRIEIVDSFHSFTKGMVLIENCNYKINRAII
                     QPATEILMLNYLKKKSMDLRIDFTHSKVMANKKFLEEGYRFSIVSQETIINNDDIIIC
                     ETFSPEMIWCLYRVKG"
     gene            597..>782
                     /locus_tag="ACL73L_002115"
     CDS             597..>782
                     /locus_tag="ACL73L_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000490588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772992.1"
                     /translation="MFSEIIKKYDIVTLKDSGFLRGDWFIGSALMAHDYESLKEGIYF
                     YPQETIWTRILDLEYINP"
BASE COUNT          283 a           77 c          152 g          270 t
ORIGIN      
        1 ttagtatctt tggaaaatca aggattagac tttgaacatt gtattttgag cttgggggaa
       61 agatgataat aaatacatta ggaccaactg caacagattg tttttatgca ataaatcgta
      121 taaaattaaa agtagatgtg attgtactgt atgatagttt tgatagtata gttaattgct
      181 ttgataaatt aaaagggcaa tatatattat tacctgtagc tttcgagagt agtattaaaa
      241 attatagttg gaaagatttc aactttgaat atcataagcg gattgaaatt gtggattctt
      301 ttcactcttt tactaaggga atggttctaa tcgagaattg taattataaa attaataggg
      361 cgattatcca acctgcaact gagattttga tgttaaacta tttaaagaaa aaaagtatgg
      421 atctgaggat agattttacc catagtaaag ttatggcaaa taaaaaattt ctggaagaag
      481 gttatcgttt ttcaatagta agccaagaaa cgataattaa taatgatgat attattattt
      541 gtgaaacttt ctctccagaa atgatttggt gcttatatag agtgaaagga tagaaaatgt
      601 tttctgaaat tattaagaaa tatgatattg ttacactgaa agatagtggt tttttgagag
      661 gtgattggtt cataggttca gcgttaatgg ctcatgacta tgaaagcctt aaggaaggga
      721 tatatttcta tccccaggaa acaatttgga caagaatctt ggatttagaa tatataaatc
      781 cg
//
LOCUS       DBERDK010000163          707 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.165, whole genome shotgun sequence.
ACCESSION   DBERDK010000163 DBERDK010000000
VERSION     DBERDK010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.165"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            1..707
                     /locus_tag="ACL73L_002116"
                     /pseudo
     CDS             1..707
                     /locus_tag="ACL73L_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436408.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          205 a          153 c          135 g          214 t
ORIGIN      
        1 ctagaattct ccttagaaag cgtcgtttca agtgctatca ttgttctaaa atgatggtcg
       61 ctgaaacttc tatcgtcaag aagaatcatc aaattcctcg tattatcaac caaaaaattg
      121 cgcaaaagtt gattgagaag atttctatga ccgatattgc ccatcagctg gccatttcaa
      181 cttcaactgt cattcgaaag ctcaatgatt ctcactttga gcatgatttt tcgcgtcttc
      241 ctgagattat gtcctgggac gttgaaacag tccggggagt gactgtttca atcgggagat
      301 ggagatgagc tttattgcgc aagattttaa caatctcaat atcatcactg ttcttgaagg
      361 tagaacacaa gctgtcatcc gagatcactt tcttaaatat gatagagccg tccgatgtcg
      421 cgtcaaaatc attactatgg atatgtttag cccttactat gacttagcta aacagcttcg
      481 ctttcaaatt tctaggctca ggctgaaaca gtctcccagg ctgtttcact cccgaatgct
      541 aaaatcgttc ttgatcgctt tcacattgta caacatctta gccgtgctat gagtcgtgtg
      601 catgtccaaa tcatgaatca gtttcatcga aaatcccatg aatacaaggc tatcaagcgt
      661 tactggaaac tcattcaaca ggatagccgt aaactgagtg ataagcg
//
LOCUS       DBERDK010000164          703 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.166, whole genome shotgun sequence.
ACCESSION   DBERDK010000164 DBERDK010000000
VERSION     DBERDK010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.166"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..>703)
                     /locus_tag="ACL73L_002117"
     CDS             complement(<1..>703)
                     /locus_tag="ACL73L_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGS0772993.1"
                     /translation="WFDLNGSLLNYSISYSAMAFSGMFAGILGRRINYFNLRNLIRIG
                     VILYSIGLLLRLFPNDWLISLISGLISGIGAATIILLLRTWAVGIGKEKDRVVTISLK
                     RFSSSLGTSIGGILSGILLYCLIYFFHSMAYPIVLASASMLCLVTFFCIPKIDSFVDK
                     SERAERILESSNYMKKRIIFFGIFSGLIASIFSPYLPVILRRQEISEVFIGPVLTILS
                     LLTIYFSSIFSSRKVD"
BASE COUNT          277 a          126 c           85 g          215 t
ORIGIN      
        1 atctactttt ctacttgaaa atatggaaga gaaataaatt gttaatagac ttaaaatagt
       61 tagaacaggc ccaataaaaa cttctgatat ttcttgacgt cttaaaataa cgggtagata
      121 aggagaaaat atactagcaa ttaaacctga gaagatacca aaaaagatta ttctcttttt
      181 catgtaatta ctagattcta agattctttc tgctctttca ctcttatcaa caaaactgtc
      241 aattttagga atacaaaaga aagttactaa acatagcata cttgccgaag ctaaaacaat
      301 aggatacgcc atactatgaa aaaaatatat taagcaatat agcaaaatgc ctgacaatat
      361 ccctccaata gaagttccta aactgctgga aaatcttttt aaggaaatcg taactactct
      421 gtctttttct tttcctattc cgacagccca tgttcgcaat aaaagaatta ttgttgccgc
      481 tccaatacct gagataagac cagagattaa tgatatcaac caatcattag gaaataatct
      541 caataataaa ccgatagaat atagtattac tcctattctt atcagatttc ttaaattaaa
      601 ataattaatt cttctgccta gaattccagc aaacattcct gaaaaagcca ttgctgaata
      661 tgaaatacta taattcagta aacttccatt caaatcaaac caa
//
LOCUS       DBERDK010000165          691 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.167, whole genome shotgun sequence.
ACCESSION   DBERDK010000165 DBERDK010000000
VERSION     DBERDK010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.167"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            1..691
                     /locus_tag="ACL73L_002118"
                     /pseudo
     CDS             1..691
                     /locus_tag="ACL73L_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397244.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
BASE COUNT          265 a           70 c          132 g          224 t
ORIGIN      
        1 ttaattttaa gtaatgaaga tttaggtgca actaatgcca gaaacatagg tatcaaaaat
       61 tctagaggta agtatatatc atttttagac gatgatgatg aatatatgcc agatcgaatt
      121 ttaaagttga tggcttgttt taaaaagagt agaatgaaga atttagcttt agtttatagt
      181 tatggaataa taatttatcc aaatggtaca cgagaagagg agaagaccga ttttgttgga
      241 aatcccttgt ttgttcaaat ggttcacaat atagcaggta cgtcattttg gttgtgtaaa
      301 aaagaggtgc tagaattaat taatggtttt gagaaaatag attcacatca ggacggtgtt
      361 gttttattaa aactacttgc tcaaggatac caaattgata tagtgcgaga attcttggtg
      421 aattactacg ctcacagtaa agaaaacggt atcactggag tgacacaaaa aacaattaat
      481 gcagatgaag aatattataa ttactgtagg aaatatttta atttattgag tttcaacgag
      541 agaatattgg ttacaaagaa atattattct ttaaacataa agcggttact attaatagga
      601 gacaaatgca aggctttaaa agtaatcaag aaggcaagag aagaaaaaat ttttaacgaa
      661 tttctttttt gaaatatatg ttattatatc a
//
LOCUS       DBERDK010000166          689 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.168, whole genome shotgun sequence.
ACCESSION   DBERDK010000166 DBERDK010000000
VERSION     DBERDK010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.168"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..561)
                     /locus_tag="ACL73L_002119"
     CDS             complement(<1..561)
                     /locus_tag="ACL73L_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HGS0772994.1"
                     /translation="MQIKFIDKVSNLIMLNLLYVASVVTVIAIGSGESALIATLIKIV
                     RHEESYPYRDFANSFFKDYWKNLGAALISNLPILILLFSLFFLPYIPLPIYIISILRH
                     IGVIYIILHLIATTFLIPLIGRYNNTLKNSLHNSIMLAYKHFFIAVLIRIIEIIPVLL
                     FFILQNQLLVWITLMIFILPSITKYAN"
BASE COUNT          267 a           75 c          108 g          239 t
ORIGIN      
        1 attagcatat tttgttattg agggaagtat aaaaatcatt aaagttatcc aaactagtaa
       61 ttgattctgt aatataaaga agagtagaac tgggataatt tcaataattc taataagaac
      121 ggcaataaaa aaatgtttat aggctaacat gattgaattg tgtagagaat tttttaaagt
      181 gttattatat ctaccgatta aaggaattag aaatgttgtt gctattaaat gaagtatgat
      241 atatattact ccaatatgtc gtaagataga aatgatatat ataggtaatg gtatgtaggg
      301 taggaagaac aaactgaata ataagattaa aattggtaaa ttagatatta atgctgctcc
      361 taaatttttc caataatctt taaaaaaact gtttgcgaag tctctatagg gataggattc
      421 ctcgtgtcgt acaattttta ttagagttgc tattagggca ctttcacctg atccgatagc
      481 aataactgtt actactgacg ctacatagag tagatttaac attataagat ttgaaacttt
      541 atcaataaat tttatctgca tctttaactc ctgctgtttt tatacttata atatcacatc
      601 atttgagttt catcaacttt gaatacatta agaaagaata taactatgag gaaaggaaaa
      661 aattattatg aataaatcca aaaaatata
//
LOCUS       DBERDK010000167          667 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.169, whole genome shotgun sequence.
ACCESSION   DBERDK010000167 DBERDK010000000
VERSION     DBERDK010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.169"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            1..667
                     /locus_tag="ACL73L_002120"
                     /pseudo
     CDS             1..667
                     /locus_tag="ACL73L_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          201 a          144 c          105 g          217 t
ORIGIN      
        1 gactttcaaa aaccttctaa aattccttat cttgaaacaa ctggtatgcc tactagaatt
       61 ctccttagaa agcgtcgttt caagtgctat cattgttcta aaatgatggt tgctgaaact
      121 tctatcgtca agaagaatca ccaaatccct cgtattatca accaaaaaat tgcgcaaaag
      181 ttaattgaaa agatttctat gaccgatatt gcccatcagc tttccatctc aacttcaact
      241 gttattcgta agctcaatga ttttcacttt aaacatgatt tttcttgtct tcctgagatt
      301 atgtcttggg atgagtatgc ttttacaaaa ggaaagatga gcttcattgc gcaagatttt
      361 aacaatctca atatcatcac tgttcttaag ggaagaacac aagctgtcat ccgaaatcac
      421 tttcttaaat atgatagagc cgtccgatgt cgcgtcaaaa tcattactat ggatatgttt
      481 agtccttact atgacttagc tagacagctt cgctttcaaa tttctaggct caggctgaaa
      541 cagtctccca gactgtttca ctcccgaatg ctaaaatcgt tctagatcgt ttccatatta
      601 tccaacatct cagccgtgct atgagtcgtg tgcgtgtcca aatcatgaat cagtttcatc
      661 gaaaatc
//
LOCUS       DBERDK010000168          644 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.171, whole genome shotgun sequence.
ACCESSION   DBERDK010000168 DBERDK010000000
VERSION     DBERDK010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.171"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
BASE COUNT          262 a           92 c           97 g          193 t
ORIGIN      
        1 ttttactata tttgattaat tattaaagaa gaaggaaaaa cacagtttta aaatactaaa
       61 cttacaatca aaaaagaatc taagctaaac tttgaagatt aaagaacagg tacatataga
      121 gttccaatat aacagaacag atacgaaatt aaatttatgc tattgttaga ttttagatta
      181 gaagatgaag agtatttaga atgcttcatg caaactaata aaacaaatat taaaagatgt
      241 gcaatagctt cctattaaat agaaacaaaa aagcactttt aaatagtgct agtttcttgc
      301 ctgctgaact cgtttatcat gttcagtgat ttgtggagta agggagaaac tccaaaacgt
      361 tcaaacgaaa agtgaaaact ccccatcacc ctattatcaa gcattaagaa gtaataaagc
      421 tacttgctga atacttaaaa aagcgataca acaaaatgtt gtaatatttt attaaatatg
      481 ataggctgta aagccttgaa actaagcact ttaggcctct atcctagagt gcttttttga
      541 tttctcttag tatccagcta taatcgttga gacataacta gaccgatata gtccaaagtg
      601 atatagtaaa atgaaccaaa aatagtacac aatgtggtat aatc
//
LOCUS       DBERDK010000169          642 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.172, whole genome shotgun sequence.
ACCESSION   DBERDK010000169 DBERDK010000000
VERSION     DBERDK010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.172"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            81..377
                     /locus_tag="ACL73L_002121"
     CDS             81..377
                     /locus_tag="ACL73L_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uberolysin/carnocyclin family circular
                     bacteriocin"
                     /protein_id="HGS0772995.1"
                     /translation="MKSKRMEFHNKFLIVSAMLAVISWLSLGVVSFPMLAGTLGISTK
                     AAATVVNLISAYSTVTAVISIVGAITGVGSIGSGIAATVLYILKKKGAAKAALW"
     gene            384..>642
                     /locus_tag="ACL73L_002122"
     CDS             384..>642
                     /locus_tag="ACL73L_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS0772996.1"
                     /translation="MKILSKKECESILGYLNFIGITLLFFVIISLSIYFKSYLPFQGK
                     LFLLFLVVDYATLNRYNMKLPIQSKKINTFYIRKNYIFLYLL"
BASE COUNT          214 a           82 c          110 g          236 t
ORIGIN      
        1 tttgaagaga gttaggaata caacatgtgc gaattaactc tcaataaaac atacataata
       61 tacaaaagga gtacactaaa atgaaatcta aaagaatgga atttcataat aaatttctta
      121 ttgtttcagc aatgttagca gtcattagtt ggttaagctt aggtgttgtt tcttttccta
      181 tgttggctgg gacattagga atttcgacaa aagctgctgc tactgtagtc aatttgatta
      241 gtgcttatag tacagttaca gcagtaattt caattgtagg tgcaatcaca ggagtaggtt
      301 ctattggttc gggtattgca gcaacagttc tttatatatt gaaaaagaaa ggtgctgcta
      361 aagcagctct atggtagaac tagatgaaaa tactttctaa aaaagaatgt gaaagtattt
      421 taggttatct taattttatt gggataaccc ttttgttttt tgttatcata agtttgtcaa
      481 tctattttaa aagctatctt ccttttcaag ggaaattatt tttacttttt ttggtagtag
      541 attacgctac tttgaatcgt tataatatga aactgcctat ccaaagtaaa aaaataaata
      601 ctttctatat aaggaaaaac tatatttttc tctatcttct tg
//
LOCUS       DBERDK010000170          622 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.173, whole genome shotgun sequence.
ACCESSION   DBERDK010000170 DBERDK010000000
VERSION     DBERDK010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..622
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.173"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            1..622
                     /locus_tag="ACL73L_002123"
                     /pseudo
     CDS             1..622
                     /locus_tag="ACL73L_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844241.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          182 a          135 c          126 g          179 t
ORIGIN      
        1 atattcatcg tctcaagaaa aaacagtctg attggaaagt acaatacact cgttgtcttg
       61 atatcatctt tcctgagttg gataaaatcg ttggaaagca ttcagaatat acctaccaac
      121 tcttgacacg ctaccctaat cctcagaaaa ggattgaggc aggatttgat aagctgatag
      181 aaattaagca ttgaccgctt ctaaaattca ggatatcctc tcagtcgcac ctcgttctat
      241 cggaacaaca tctcctgctc gtgaattcga aatcatcgaa atcatcaaac attacaagag
      301 actcattgac aaggcggaaa catgtgtcaa tgacttgatg gctgagttca actcggtcat
      361 cacgacggtt actgggattg ggaatcgttt aggggcggtc attttagccg agattcgaaa
      421 tattcatgcc tttgataatc ctgctcaatt acaagctttc gctggactgg attcttctat
      481 ttatcagtca ggtcagattg atttagctgg aagaatgatc aaacggggtt cccctcatct
      541 gcggtgggca ctcatacaag ctgccaaagc atgcgctcgc ttttcacctg cttttaaggc
      601 ctatcttaag actaagttag aa
//
LOCUS       DBERDK010000171          597 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.174, whole genome shotgun sequence.
ACCESSION   DBERDK010000171 DBERDK010000000
VERSION     DBERDK010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.174"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..>597
                     /locus_tag="ACL73L_002124"
     CDS             <1..>597
                     /locus_tag="ACL73L_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGS0772997.1"
                     /translation="EYQIKNLSYKFTDDECLSLKDISFKLGKIYGIIGSNGRGKSTLL
                     RCLIGLEKKSKEEIYFKGEKLSKKERLKNSSLVMQDVNHQLFTDEVFNELRLGVKNFD
                     EEKAKIILKDLGLDEFIERHPMSLSGGQKQRLAIASVMCKNSPFVFFDEPSSGMDYSN
                     MIKISELINKYKTMDKIIFIVSHDIEFLNEVADEIFEL"
BASE COUNT          232 a           70 c          112 g          183 t
ORIGIN      
        1 agagtatcag ataaaaaatc ttagctacaa atttactgat gatgagtgct taagcttaaa
       61 agatatttcg ttcaagcttg ggaaaattta tggcataata ggatccaacg gacgaggaaa
      121 atcaacgctt ttaagatgtt taataggtct tgagaaaaaa tcaaaagaag aaatttattt
      181 taagggagag aagctatcta aaaaagaaag actcaaaaac tcttcacttg ttatgcaaga
      241 tgtaaatcat caattattca cagatgaagt attcaacgag cttagattag gagtaaagaa
      301 ttttgatgaa gaaaaggcga aaatcatttt aaaagattta ggcctggacg aatttattga
      361 aaggcatccg atgagtttat caggagggca aaagcaaagg cttgcaatag catctgttat
      421 gtgcaagaat tctccatttg tcttttttga cgaaccttca agtggtatgg attattccaa
      481 tatgataaaa atatctgaac tgattaataa gtataaaacc atggataaaa taatttttat
      541 tgtttcccat gatatagaat ttttaaatga agtggcagat gaaatttttg aattata
//
LOCUS       DBERDK010000172          593 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.175, whole genome shotgun sequence.
ACCESSION   DBERDK010000172 DBERDK010000000
VERSION     DBERDK010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.175"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..591)
                     /locus_tag="ACL73L_002125"
     CDS             complement(<1..591)
                     /locus_tag="ACL73L_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGS0772998.1"
                     /translation="MKIENESKSYDKCILKNISLEIDKGIYRVEGENGSGKSTLLQLL
                     AGLETFDSGLKNCVSKDVLYLDTNQLGVVPFTIEENLQLLCDSFQIHLSFEDKEQINT
                     FFDNKIGDNYMTASTGTKFKLGLSLIFAKNWDYIFIDETLSTVDARSIDMIAKRLISM
                     KESSTIIYVSHNLLNQELISKSQRILIEEGRVYEISN"
BASE COUNT          201 a          106 c           70 g          216 t
ORIGIN      
        1 gtttgaaatt tcataaaccc gtccttcctc tattaaaatc ctttgagatt tgctaatcaa
       61 ttcttgattc aataaattat gagatacata tataatagta gagctttctt tcatggaaat
      121 taagcgttta gcaatcatat caatacttct tgcatccact gttgataaag tctcatcaat
      181 aaaaatataa tcccaatttt tagcaaaaat taaggataag cctaatttga attttgttcc
      241 agtagatgct gtcatatagt tatctccgat tttattatca aaaaaagtat taatttgttc
      301 tttatcctca aatgatagat ggatttgaaa agaatcacac aacaactgca aattttcttc
      361 tatagtaaat ggaacaaccc caagctgatt ggtatcaagg tatagtacat ctttggagac
      421 acaattcttt aaaccactat caaaagtctc tagcccagct aaaagttgta gaagggtgct
      481 ctttcctgaa ccattctctc cttcaactct gtaaattccc ttatcaatct ctagtgatat
      541 atttttcaat atacatttat cataactttt agattcattt tcaattttca taa
//
LOCUS       DBERDK010000173          510 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.177, whole genome shotgun sequence.
ACCESSION   DBERDK010000173 DBERDK010000000
VERSION     DBERDK010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.177"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..401
                     /locus_tag="ACL73L_002126"
     CDS             <1..401
                     /locus_tag="ACL73L_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HGS0772999.1"
                     /translation="YDEKIGSFNTYEIEQTVSKILRVKPNGKIILKSTVPIGFSNKLK
                     RLFDTKNIIFVPEFLREGCSIYDNLYPSRIVVGDETVEGRKIAELFLSISTHSTANIK
                     NVMLVSPTEAEAIKLFSNTFLALRVAFLMN"
     gene            443..>510
                     /locus_tag="ACL73L_002127"
     CDS             443..>510
                     /locus_tag="ACL73L_002127"
                     /inference="COORDINATES: protein motif:HMM:NF013175.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HGS0773000.1"
                     /translation="MKGVCLDPRIGNFYNNPSFGFGG"
BASE COUNT          169 a           63 c           98 g          180 t
ORIGIN      
        1 attatgatga gaaaattggt agttttaata catatgaaat cgaacaaacg gtatcgaaga
       61 ttctgagggt aaaacctaat ggaaagatta ttttaaagtc aacagttccg atcggctttt
      121 caaacaaatt aaaaaggctg tttgatacaa aaaatatcat ttttgtccct gaatttttga
      181 gagaaggttg ttctatatat gataatttat atccaagtcg catagttgtt ggagatgaga
      241 cagttgaagg aagaaaaatt gcagagttgt tcctttcgat tagtactcat agtactgcca
      301 atattaaaaa tgttatgtta gtttctccta ctgaagcaga agcaattaag cttttttcta
      361 acacattctt agctctccgt gttgcttttt taatgaacta gattcttttg ctgagaggag
      421 aagcttaaat gctgaagttg taataaaagg tgtttgttta gatccaagaa ttggaaattt
      481 ttataataat ccttcttttg gatttggagg
//
LOCUS       DBERDK010000174          372 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 465385
            SAMN38107269-rid24759253.denovo.182, whole genome shotgun sequence.
ACCESSION   DBERDK010000174 DBERDK010000000
VERSION     DBERDK010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107269
            Sequence Read Archive: SRR26673591
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570813.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570813.1
            Genome Coverage       :: 163x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 08:36:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,128
            CDSs (total)                      :: 2,091
            Genes (coding)                    :: 1,940
            CDSs (with protein)               :: 1,940
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 82 of 151
            Pseudo Genes (incomplete)         :: 71 of 151
            Pseudo Genes (internal stop)      :: 48 of 151
            Pseudo Genes (multiple problems)  :: 42 of 151
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107269-rid24759253.denovo.182"
                     /strain="GPSC46"
                     /isolate="465385"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            85..>372
                     /locus_tag="ACL73L_002128"
     CDS             85..>372
                     /locus_tag="ACL73L_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGS0773001.1"
                     /translation="MSEYWFSTNVDQIDEVDGKQCLIYSYYNIKASRNVEVLKGRSGT
                     KKGLDYWEPYAPQKQYEMERLPKNKYIGSSSTDRWDGIEKNVVFCDCKEYVS"
BASE COUNT          139 a           43 c           70 g          120 t
ORIGIN      
        1 cctattactt tattagttgg tagaaatgga gtaaataaaa cttcaatttt acaagcttta
       61 tatggttctc cagccaataa aagtgtgtct gaatattggt ttagtacaaa tgttgatcaa
      121 atagatgaag tggatgggaa acaatgttta atctattcat attataacat taaagcttct
      181 agaaatgtag aagttcttaa aggccgttct ggaacaaaaa aaggattaga ttattgggaa
      241 ccatatgctc ctcaaaaaca atatgagatg gaaagattac ccaaaaataa atatattgga
      301 agttcttcga cagacagatg ggatggcatt gaaaaaaatg ttgtcttttg tgattgtaaa
      361 gaatatgttt ct
//
